<?xml version="1.0" encoding="UTF-8"?>
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 xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance"
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<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YH34</accession>
    <name>A0AA38YH34_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_026712</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YH34"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009941">
        <property type="term" value="C:chloroplast envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040320">
        <property type="entry name" value="At4g37920-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31755">
        <property type="entry name" value="FOLATE RECEPTOR-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31755:SF2">
        <property type="entry name" value="OS08G0320800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9670333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="431" mass="49278" checksum="9C7A28B254657846" modified="2024-01-24" version="1">MSNLLGFKLLLCNTTKPSVLNQNLFSASILLPSISSPPLFLPSKQSNCLTPNSRTRKGRATSHTVLSNFRCNSTANSIGAAEVAEQVEVEVAEGYTITQFCDKIIDVFMNEKPKSKEWRKYLVFRDEWNKYREAFYNRCQTRAYAETDPVMKKKLIELGRKVKKIDDEMERHTELLKEVQSSPMDVNAIVVRRRKDFTGEFFRHLSLLSETYDSLEDRDAMARLGARCLSAVSAYDNTLEIVETLDVAQAKFDDILNSPSIDVACEKIESLAKAKELDSSLILLINSAWSAAKESTTMKNEVKDIMYHLYKATKSSLRSIAPKEIKLLKHLLNITDPEERFSALASAFSPGDDHEAKDPNALYTTPKELHKWIKIMLDAYHLNKEETDIREARQMTEPVVIQRLFILKETIEEEYLERGIKTEQETKSEEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YKA7</accession>
    <name>A0AA38YKA7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_025583</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YKA7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22157">
        <property type="entry name" value="F-box_AtFBW1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013187">
        <property type="entry name" value="F-box-assoc_dom_typ3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017451">
        <property type="entry name" value="F-box-assoc_interact_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01640">
        <property type="entry name" value="F_box_assoc_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31111">
        <property type="entry name" value="BNAA05G37150D PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31111:SF136">
        <property type="entry name" value="F-BOX ASSOCIATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08268">
        <property type="entry name" value="FBA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="20"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="F-box associated beta-propeller type 3" evidence="2">
        <location>
            <begin position="105"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9672002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="375" mass="43718" checksum="EFF902198F4352FB" modified="2024-01-24" version="1">MDDLCNKLICTRNENLFDVEDIVINILSRLPVKSLMICKSVSKQWWRLISSPNFMRLQLIRSQENPIYVFYPYSCRCHSEHFLTKIDGETTETLPGCNRHYFRGMICSFNGLICCINTEDIRGMIRCRRIRRLDIHICNPATREVLLLPQSRGSKESPKIGVAFYPRTNEYKVFRFFNPAGKSHDKHSECEVYSSITGCWKGIGCVAHSPWSSNHICINGTVYWFIRSEKDGLIIVSILAVDMEENFSVIGIPEEATLRTFLVSFEGCLSLVAFHVFDKNRFHMWILQDSKKSTWVRKCSDYMSILNIKQVQHVAVQKNEILFASVQQYFFYNMCSRTWREFNWVHDFRKRLSIPLAYTESLLPLCLISHCPLGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YLA1</accession>
    <name>A0AA38YLA1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cytochrome c oxidase assembly protein COX15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_025927</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>Fe(II)-heme o + 2 A + H2O = Fe(II)-heme a + 2 AH2</text>
            <dbReference type="Rhea" id="RHEA:63388"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:60530"/>
            <dbReference type="ChEBI" id="CHEBI:61715"/>
            <dbReference type="EC" id="1.17.99.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:63389"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="10">Porphyrin-containing compound metabolism; heme A biosynthesis; heme A from heme O: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YLA1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120547">
        <property type="term" value="F:heme A synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016653">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, heme protein as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006784">
        <property type="term" value="P:heme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01665">
        <property type="entry name" value="HemeA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003780">
        <property type="entry name" value="COX15/CtaA_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023754">
        <property type="entry name" value="HemeA_Synthase_type2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23289">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23289:SF2">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX15 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02628">
        <property type="entry name" value="COX15-CtaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="180"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="210"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="248"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="292"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="355"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="384"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="414"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9672512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="452" mass="50295" checksum="B687B423977E4E68" modified="2024-01-24" version="1">MFGRQLVSILRRNKLQNCFFNESSSRVLSKRPRLFSSELGGSSGAQRNALYGFKSLIKDFHKPWLRNMSTTATATVENKEGLKLLVTAGARAQKMVGIWLFGSAAWVFSMVVLGGVTRLTRSGLSMTDWKFTGNLPPLSDEDWLAEFEKYKQSPEYKRVNKGMSIEDFKFIYWMEYAHRMWGRALGIMFALPFSYFLHKGYITLRLGLRLSALFGLGAGQGLIGWWMVKSGLEEPASEYAQPRVSPYRLTAHLTSAFVIYCGLLWTALSVVMPEPPAESVAWVRGAAKVKRLALPISILVGITAVSGAFVAGNDAGHAYNTFPKMGDNWIPNDIFSMEPFIRNFFENTSTVQLDHRILATTTLMSIGILWWSTRKLDIHPAVRFLIGNTIGMAALQVTLGISTLLSYVPVSLGTAHQAGALTLLTLMVLLNHTVRRPSMSLLKSLPPVTRTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YS85</accession>
    <name>A0AA38YS85_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Light-harvesting complex-like protein OHP2, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_024449</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YS85"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053091">
        <property type="entry name" value="PSII_Assembly/Photoprotect-Rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37752">
        <property type="entry name" value="OS02G0610700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37752:SF1">
        <property type="entry name" value="OS02G0610700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103511">
        <property type="entry name" value="Chlorophyll a-b binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="105"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9675524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="217" mass="23701" checksum="CF7367234F4FEBDA" modified="2024-01-24" version="1">MATSHPHTHSSILITRTTRRLQSTPFCSSHSLSFWILSISKSEKKSKKMSVASSIPCIKIPTSSSSSSSLSSSYYRFSATKPGIFTIRSSQTEGPLRRPVAPLREPAPPPLPPSPSPSAPPPPSPPPLPSASVDKNAVTLEFQRQVAKELQDYFKQKKLEEANQGPFFGFIGKNEISNGRWAMFGFAVGMLTEYATGSDFVDQVKILLSNFGIIDLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YVU5</accession>
    <name>A0AA38YVU5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_022561</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Functions as an E3 ubiquitin ligase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YVU5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000578">
        <property type="entry name" value="RING-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000565">
        <property type="entry name" value="RING-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23315">
        <property type="entry name" value="U BOX DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23315:SF276">
        <property type="entry name" value="U-BOX DOMAIN-CONTAINING PROTEIN 38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04564">
        <property type="entry name" value="U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51698">
        <property type="entry name" value="U_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="U-box" evidence="10">
        <location>
            <begin position="22"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="8">
        <location>
            <begin position="287"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="8">
        <location>
            <begin position="328"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9677643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="523" mass="56710" checksum="6DAE4A30C993D1B9" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YX87</accession>
    <name>A0AA38YX87_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 13">Phenylalanine ammonia-lyase</fullName>
            <ecNumber evidence="6 13">4.3.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_020524</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This is a key enzyme of plant metabolism catalyzing the first reaction in the biosynthesis from L-phenylalanine of a wide variety of natural products based on the phenylpropane skeleton.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-phenylalanine = (E)-cinnamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:21384"/>
            <dbReference type="ChEBI" id="CHEBI:15669"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="EC" id="4.3.1.24"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 13">Phenylpropanoid metabolism; trans-cinnamate biosynthesis; trans-cinnamate from L-phenylalanine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the PAL/histidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.1.24" evidence="6 13"/>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YX87"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045548">
        <property type="term" value="F:phenylalanine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006559">
        <property type="term" value="P:L-phenylalanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009698">
        <property type="term" value="P:phenylpropanoid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00332">
        <property type="entry name" value="PAL-HAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.274.20:FF:000001">
        <property type="entry name" value="Phenylalanine ammonia-lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.275.10:FF:000009">
        <property type="entry name" value="Phenylalanine ammonia-lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.200.10:FF:000009">
        <property type="entry name" value="Phenylalanine ammonia-lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.200.10">
        <property type="entry name" value="Fumarase/aspartase (Central domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="entry name" value="Fumarase/aspartase (N-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.274.20">
        <property type="entry name" value="Phenylalanine ammonia-lyase 1, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001106">
        <property type="entry name" value="Aromatic_Lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022313">
        <property type="entry name" value="Phe/His_NH3-lyase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005922">
        <property type="entry name" value="Phe_NH3-lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023144">
        <property type="entry name" value="Phe_NH3-lyase_shielding_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01226">
        <property type="entry name" value="phe_am_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10362">
        <property type="entry name" value="HISTIDINE AMMONIA-LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00221">
        <property type="entry name" value="Lyase_aromatic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="L-aspartase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00488">
        <property type="entry name" value="PAL_HISTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-0585">Phenylalanine catabolism</keyword>
    <keyword evidence="8 13" id="KW-0587">Phenylpropanoid metabolism</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9678375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="710" mass="77281" checksum="EF5E820998A53098" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YXX1</accession>
    <name>A0AA38YXX1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_020576</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YXX1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9678434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="141" mass="16368" checksum="76146187F55CE725" modified="2024-01-24" version="1">MEYHSTNVTPLQDSREIPTVGSSLITRQNTSQMTNQPFPSLDQSFMIKLDCNNFLIYGFDDILDGTLPWPPHFLLDPNSTFATTIKPIVVNPKYITWQYQNRLVMSWIYFLLTEPMMTQIMSYNTTHEIRESLRCSFASTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YY85</accession>
    <name>A0AA38YY85_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_020866</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YY85"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9678800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="172" mass="18568" checksum="FB16740BA29B48AD" modified="2024-01-24" version="1">MAPKRTQVVHVNKGNDKIVITQSTHDTATGPMTRSKAKSTSSISTKQISESACLLKQVRTHDEHQPFITLVSWGAKSQSPRSREKLPSTLQDFGDKSPCSVTDANSSTGSHSGSPAGMSKEENYSNHSDSFTSHFSVTMPVMATGTTSVEEQLAEMAHAIAKLTKTVEDKDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YZE7</accession>
    <name>A0AA38YZE7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_021228</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the AP2/ERF transcription factor family. ERF subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YZE7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045277">
        <property type="entry name" value="DRE1A-I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31839:SF85">
        <property type="entry name" value="AP2_ERF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31839">
        <property type="entry name" value="DEHYDRATION-RESPONSIVE ELEMENT-BINDING PROTEIN 1D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="9">
        <location>
            <begin position="72"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="145"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="36"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="147"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9679229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="237" mass="25666" checksum="C1F7C6E0DBE7C88A" modified="2024-01-24" version="1">MDERRANVRQSDQPPPTPKIEVPDSSLSIVPPQDLASPHSHSSSTPTSAHPSPKNLPSPTPFSGQTAGKHPFYRGIRCRGNKWVSEIREPRKTTRIWLGTYPTPEMAATAYDVAALALKGTDATLNFPNSILSYPIPKSTSASDIRAAAARAAEALQAKPESSESPNPTQPKTEETTESSSLAAGEDQFIDEEELLNMPNLLKDMAEGMLVSPPRLKSQPSDDSPENSDSDSLWSYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z0G8</accession>
    <name>A0AA38Z0G8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UFSP1/2/DUB catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_021692</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z0G8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012462">
        <property type="entry name" value="UFSP1/2_DUB_cat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07910">
        <property type="entry name" value="Peptidase_C78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UFSP1/2/DUB catalytic" evidence="2">
        <location>
            <begin position="128"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9679857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="339" mass="38478" checksum="F25FA87184EDA9E0" modified="2024-01-24" version="1">MHSSPCPFCHLTFPASQLQRHANDHLEEEELRRDMELARQIAIAPTSPPKTLNNPVENGMLVEISSGKTYERSSSRSDESSNCSGEMSIDEKVFHLARLQIRSTFYRVEDGLMVLLRNCLESELGNSTCILSGSVIHFQSIESEDVGWGCGWRNIQMLSSHLLMQRQEARQVLFGGSGFIPDIPSLQRWLEIAWERDFDTVGSEHFSHKIYGTTNWIGTTECAALFRSFGLRARIVDFGPKEIKSPYPQKNSQGKRKAHEVCGPIDKFLLQRESRDQDISQPGSSRHDSCNLADITESDKCFTRNDKGRQVLVDWVWNYFSDNKFTKSGSHDVIVTDKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z169</accession>
    <name>A0AA38Z169_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_019491</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z169"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9680203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="41" mass="4471" checksum="8B8BF54AADF40097" modified="2024-01-24" version="1">MLLDLIEFKFQNKPNSALQAHPITTKVAAASLLVFVLAFGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z1T2</accession>
    <name>A0AA38Z1T2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NAD-dependent epimerase/dehydratase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_019952</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z1T2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08958">
        <property type="entry name" value="FR_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000085">
        <property type="entry name" value="Dihydroflavonol reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF563">
        <property type="entry name" value="CINNAMOYL-COA REDUCTASE 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="4">
        <location>
            <begin position="8"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9680790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="327" mass="36208" checksum="A7250B00A84B4E9E" modified="2024-01-24" version="1">MEGDKGRVCVTGGTGFIASWLVMKLLQHGYSVNATIRSHPQSKKDVSYLTNLPGASEKLRIYHADLSDPSSFEAAIEGCIGVFHVAHPIDFEDTEPEETVTKRSVEGTLGILKACLNSKTVKRVVYTSSTAAVEYNDKGGDIKDESSWSDVDFLKALNYWGQSYMISKTITERAALDFAQEHGLDLVTVIPSFVVGPFICPRFPGSVNTSLALVLGDQQQYHILMSIPMVHVDDVCSAHIFLLEYPDAKGRYICSSDRLTLNEMSEFLSAKYPQLPIPTIDSLKEIQGYRTPGVSSKKLLDTGFKYKYGLDEMFDDAIQCCKEKGFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z229</accession>
    <name>A0AA38Z229_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">LOB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_019940</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LOB domain-containing protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z229"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009755">
        <property type="term" value="P:hormone-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004883">
        <property type="entry name" value="LOB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31529">
        <property type="entry name" value="LOB DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31529:SF28">
        <property type="entry name" value="LOB DOMAIN-CONTAINING PROTEIN 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03195">
        <property type="entry name" value="LOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50891">
        <property type="entry name" value="LOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LOB" evidence="3">
        <location>
            <begin position="10"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="91"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9680777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="194" mass="21003" checksum="C3D8D468E2880853" modified="2024-01-24" version="1">MTGSKGGSGGPCGACKFLRRKCVKGCVFAPYFDSDQGTAHFAAVHKVFGASNASKLLTGIPAHKRLDAVVTLCYEALARVRDPVYGCVAHVFTLQQQVMNLQAELAFIQARLSTLQRIPSLALPPLVPESAPQPNLQSDFFNVPLQSHEASMEAADFCNNSMDRELEDGDLQTLAQEFVSRFLPGVKVRDSSSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z264</accession>
    <name>A0AA38Z264_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein ARV</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_020078</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Mediator of sterol homeostasis involved in sterol uptake, trafficking and distribution into membranes.</text>
    </comment>
    <comment type="function">
        <text evidence="10">Regulates also the sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Endoplasmic reticulum membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ARV1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z264"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032366">
        <property type="term" value="P:intracellular sterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097036">
        <property type="term" value="P:regulation of plasma membrane sterol distribution"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006665">
        <property type="term" value="P:sphingolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016125">
        <property type="term" value="P:sterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007290">
        <property type="entry name" value="Arv1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14467">
        <property type="entry name" value="ARV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14467:SF0">
        <property type="entry name" value="PROTEIN ARV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04161">
        <property type="entry name" value="Arv1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 10" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="119"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU368065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9680986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="248" mass="28292" checksum="240A2CFBFFA025EF" modified="2024-01-24" version="1">MELRCIHCGFPIKTLFLQYSPGNIRLMKCENCKAVADEYIECESMIVLIDLILHKPKAYRHLLFNLLNPQTLNFQGLLWKSALGFLLLDAYRIMVLTRSEEEWGLSMSFSSLFWRFSKILMDVLFGNLAFLSIFLLATRNLLYTVGGASRYKNLLLAILISSYFKIFLIAMMVWELPSSVIFIIDVFVLSSNTVALKVVTGSGMSRCAETCFVAHAVKLFVGQLTVFHSSRFLQDSKILTCEMPIYSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z293</accession>
    <name>A0AA38Z293_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Methyltransferase</fullName>
            <ecNumber evidence="8">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_020050</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endomembrane system</location>
            <topology evidence="7">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
            <topology evidence="1 8">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the methyltransferase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="8"/>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z293"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000165">
        <property type="entry name" value="probable methyltransferase PMT13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004159">
        <property type="entry name" value="Put_SAM_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10108:SF763">
        <property type="entry name" value="PECTIN METHYLTRANSFERASE QUA3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10108">
        <property type="entry name" value="SAM-DEPENDENT METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03141">
        <property type="entry name" value="Methyltransf_29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9680930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="597" mass="67157" checksum="B79F2EFCE4F70C87" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z2P3</accession>
    <name>A0AA38Z2P3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Plant bHLH transcription factor ACT-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_020093</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9681012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z2P3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010052">
        <property type="term" value="P:guard cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054502">
        <property type="entry name" value="bHLH-TF_ACT-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044283">
        <property type="entry name" value="FAMA/SPEECHLESS/MUTE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46684:SF16">
        <property type="entry name" value="TRANSCRIPTION FACTOR BHLH67-LIKE ISOFORM X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46684">
        <property type="entry name" value="TRANSCRIPTION FACTOR FAMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22754">
        <property type="entry name" value="bHLH-TF_ACT-like_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Plant bHLH transcription factor ACT-like" evidence="5">
        <location>
            <begin position="59"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF22754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9681012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="143" mass="15368" checksum="EAFB174837BAAD4C" modified="2024-01-24" version="1">MRSEEGGDASTNSSSSSPKIASKGLCTQHRFAPDESNSAEGGRREEFTFTADNKSAAADIEVTVIQTHVNLKIQCPRRPGQLLKAIVALEDLSLTVLHLNITSLQSSVLYSFNLKIEDDCKLVSADEVAAAVHQVFSFINGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z5M3</accession>
    <name>A0AA38Z5M3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Peroxisome biogenesis protein 12</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Peroxin-12</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_018435</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Component of a retrotranslocation channel required for peroxisome organization by mediating export of the PEX5 receptor from peroxisomes to the cytosol, thereby promoting PEX5 recycling. The retrotranslocation channel is composed of PEX2, PEX10 and PEX12; each subunit contributing transmembrane segments that coassemble into an open channel that specifically allows the passage of PEX5 through the peroxisomal membrane. PEX12 also regulates PEX5 recycling by activating the E3 ubiquitin-protein ligase activity of PEX10. When PEX5 recycling is compromised, PEX12 stimulates PEX10-mediated polyubiquitination of PEX5, leading to its subsequent degradation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the pex2/pex10/pex12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z5M3"/>
    <dbReference type="SMR" id="A0AA38Z5M3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990429">
        <property type="term" value="C:peroxisomal importomer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016558">
        <property type="term" value="P:protein import into peroxisome matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006513">
        <property type="term" value="P:protein monoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16451">
        <property type="entry name" value="mRING_PEX12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000357">
        <property type="entry name" value="Peroxisome biogenesis protein 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017375">
        <property type="entry name" value="PEX12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006845">
        <property type="entry name" value="Pex_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12888:SF0">
        <property type="entry name" value="PEROXISOME ASSEMBLY PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12888">
        <property type="entry name" value="PEROXISOME ASSEMBLY PROTEIN 12 PEROXIN-12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04757">
        <property type="entry name" value="Pex2_Pex12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038074">
        <property type="entry name" value="Peroxisome_assembly_p12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 15" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Pex N-terminal" evidence="16">
        <location>
            <begin position="21"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR038074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF04757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9682513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="393" mass="44763" checksum="36D4373774975F09" modified="2024-01-24" version="1">MLFQVGGQGARPTFFEMSAAQQLPASLRAALTYSIGVLALRRPFLHRVLDYEDEFFALLMLVLESHSLRTTDASFSESLYGLRRRAVRIRVKKDNTLSDSSDVIHHSGLEKYQKRLSVLFLVVLPYFKSKLHSVYNKEREATLQASLWGHGDERFDDADYFSEERGSLIPTRASDVEVSVRARLTKRFQKFIGIFYPWLHAGNEGLSFAYQLLYLLDATGFYSLGLHALGIHVCRATGQELMDTSSRISKIRSRERDRLRGPPWLKALQGALLSCTYTMLDYAQTGLIAAVFFFKMMEWWYQSAEERMSAPTVYPPPPPPPPPKVAKEGIPLPSDRTICPLCSQKRTNPSVVAVSGFVFCYACIFKYVSQYKRCPITLMLANVDQIRRLFHDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z5S5</accession>
    <name>A0AA38Z5S5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HEAT repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_018771</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046837">
        <property type="entry name" value="Laa1/Sip1/HEATR5-like_HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044218">
        <property type="entry name" value="SWEETIE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46975">
        <property type="entry name" value="PROTEIN SWEETIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46975:SF2">
        <property type="entry name" value="PROTEIN SWEETIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20210">
        <property type="entry name" value="Laa1_Sip1_HTR5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1085"/>
            <end position="1129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1187"/>
            <end position="1293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1320"/>
            <end position="1368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1187"/>
            <end position="1202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1212"/>
            <end position="1226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1237"/>
            <end position="1253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1263"/>
            <end position="1272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1340"/>
            <end position="1352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9682911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1368" mass="152207" checksum="13BF07E4DBF1FBE8" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z8Z2</accession>
    <name>A0AA38Z8Z2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)</fullName>
            <ecNumber evidence="5">5.4.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_016869</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>O-phospho-L-seryl-[protein] + alpha-D-glucose 1-phosphate = alpha-D-glucose 1,6-bisphosphate + L-seryl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:68748"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:58392"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>alpha-D-glucose 1,6-bisphosphate + L-seryl-[protein] = O-phospho-L-seryl-[protein] + alpha-D-glucose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:68752"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:58225"/>
            <dbReference type="ChEBI" id="CHEBI:58392"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>alpha-D-glucose 1-phosphate = alpha-D-glucose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:23536"/>
            <dbReference type="ChEBI" id="CHEBI:58225"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="EC" id="5.4.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.2" evidence="5"/>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z8Z2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009570">
        <property type="term" value="C:chloroplast stroma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004614">
        <property type="term" value="F:phosphoglucomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004615">
        <property type="term" value="F:phosphomannomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03089">
        <property type="entry name" value="PMM_PGM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.120.10:FF:000014">
        <property type="entry name" value="Phosphomannomutase/phosphoglucomutase isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.120.10:FF:000010">
        <property type="entry name" value="phosphomannomutase/phosphoglucomutase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.120.10:FF:000022">
        <property type="entry name" value="phosphomannomutase/phosphoglucomutase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.120.10">
        <property type="entry name" value="Alpha-D-Glucose-1,6-Bisphosphate, subunit A, domain 3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005845">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005846">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005841">
        <property type="entry name" value="Alpha-D-phosphohexomutase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050060">
        <property type="entry name" value="Phosphoglucosamine_mutase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42946:SF1">
        <property type="entry name" value="PHOSPHOGLUCOMUTASE (ALPHA-D-GLUCOSE-1,6-BISPHOSPHATE-DEPENDENT)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42946">
        <property type="entry name" value="PHOSPHOHEXOSE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02879">
        <property type="entry name" value="PGM_PMM_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02880">
        <property type="entry name" value="PGM_PMM_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00509">
        <property type="entry name" value="PGMPMM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="Phosphoglucomutase, first 3 domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6" id="KW-0313">Glucose metabolism</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="11">
        <location>
            <begin position="99"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="12">
        <location>
            <begin position="275"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="13">
        <location>
            <begin position="382"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9684615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="619" mass="66801" checksum="F7A5C057D354C82F" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z926</accession>
    <name>A0AA38Z926_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">LOB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_016824</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LOB domain-containing protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z926"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004883">
        <property type="entry name" value="LOB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31301:SF188">
        <property type="entry name" value="LOB DOMAIN-CONTAINING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31301">
        <property type="entry name" value="LOB DOMAIN-CONTAINING PROTEIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03195">
        <property type="entry name" value="LOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50891">
        <property type="entry name" value="LOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LOB" evidence="2">
        <location>
            <begin position="62"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9684544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="186" mass="20586" checksum="E9B0776F42FF8CBA" modified="2024-01-24" version="1">MPSASVFNHKRLIVSLQLSLSLRMEYADIDKTTRPLAVSCIPFSSSSPSSSRFSPPPTAFQGPCAACRFLRRRCDESCILAPYFPPNEPLKFINAHKVFGASNIVKALQELPQPTRADAVSSMVYEANARIRDPVYGCAGTICQLHKQLNDLKAELAMAQAELCIMQCQQQQQNANTSFFLDTNLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZA97</accession>
    <name>A0AA38ZA97_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein MIZU-KUSSEI 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_017356</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZA97"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010274">
        <property type="term" value="P:hydrotropism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006460">
        <property type="entry name" value="MIZ1-like_pln"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01570">
        <property type="entry name" value="A_thal_3588"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31696:SF73">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31696">
        <property type="entry name" value="PROTEIN MIZU-KUSSEI 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04759">
        <property type="entry name" value="DUF617"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9685296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="252" mass="28120" checksum="C1301FF6FB0C5858" modified="2024-01-24" version="1">MPAPRVPPSAPPPPPPRSPTRQGPAALDPEKTPSHPKVALQQPSHKKASLKSTKLFRHFRSVFRSFPIISPACKIPVLLHNSRLNDVHIHGGTRMTGTLFGHRKGRVSLAIQESPRCFPIFLLELAIPTSKLLQELGLGLVRIALECEKRPEEKIKLMDEPIWNLYCNGRKMGYAVKREANAEDLNVMQMLHAVSMGAGVLPSEAIDLPDGELTYMRAHFQRVIGSKDSETYYMMNPDSNTGTELSIFFVRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZAE0</accession>
    <name>A0AA38ZAE0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mediator complex subunit 15 KIX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_017283</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZAE0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.246.20:FF:000003">
        <property type="entry name" value="Mediator of RNA polymerase II transcription subunit 15a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.20">
        <property type="entry name" value="Coactivator CBP, KIX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036529">
        <property type="entry name" value="KIX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036546">
        <property type="entry name" value="MED15_KIX"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16987">
        <property type="entry name" value="KIX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mediator complex subunit 15 KIX" evidence="3">
        <location>
            <begin position="2"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9685190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="91" mass="10770" checksum="DD9D54B8DA0A1D5D" modified="2024-01-24" version="1">MYMLKRHLPISRPKELCEIRKISERFEEKIYSAATSESDYLRKIPLKVLAMETKCFNHATNSLPFNSFAHRSRDRVVCQHIDLYHAAPRPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZAV9</accession>
    <name>A0AA38ZAV9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Xanthoxin dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_017505</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZAV9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010301">
        <property type="term" value="F:xanthoxin dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009688">
        <property type="term" value="P:abscisic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05326">
        <property type="entry name" value="secoisolariciresinol-DH_like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045309">
        <property type="entry name" value="ABA2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42820">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42820:SF1">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE_REDUCTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9685487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="265" mass="28080" checksum="EEEAC9AD42EDC5C0" modified="2024-01-24" version="1">MDWKVALVTGGATGIGESIVRLFLKQGAKVCIVDVQDDFGQKLCDTLGGDPNVCFFHCDVTIEDDVCHAVDFTVTKFGTLDIMVNNAGMAGPPCSDIRNVDVSMFEKIFDVNVKGVFLGMKHAARIMIPLKKGTIISLCSVSSAIAGVGPHAYTSFKHAVAGLTQSVAAEMGGHGIRVNCISPYAIATGLALAHLPEDERTEDAMAGFRAFVGKNANLQGVELTVDDIAHAAVFLASDEARYISGLNLVLDGGFSCANHSLRVFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZBS6</accession>
    <name>A0AA38ZBS6_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_015006</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9685910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZBS6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048316">
        <property type="term" value="P:seed development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00265">
        <property type="entry name" value="MADS_MEF2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1810.10:FF:000009">
        <property type="entry name" value="agamous-like MADS-box protein AGL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050142">
        <property type="entry name" value="MADS-box/MEF2_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033896">
        <property type="entry name" value="MEF2-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002487">
        <property type="entry name" value="TF_Kbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48019">
        <property type="entry name" value="SERUM RESPONSE FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01486">
        <property type="entry name" value="K-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00404">
        <property type="entry name" value="MADSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51297">
        <property type="entry name" value="K_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00350">
        <property type="entry name" value="MADS_BOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="7">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="K-box" evidence="8">
        <location>
            <begin position="87"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="87"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9685910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="225" mass="26059" checksum="52EA2405F92A5503" modified="2024-01-24" version="1">MGRGKIEIKRIENTTNRQVTFCKRRNGLLKKAYELSVLCDAEVALIVFSSRGRLYEYANNSVRTTIERYKKVCSDSSNTGSVSEANAQFYQQEASKLRRQIRDIQNLNRHILGEALSSLNFKELKNLETRLEKGISRIRSKKNELLFAEIEYMQKREIELQNSNLFLRAQIAENERAQQQMNLMPGSQYESVPQQPYDSQNLLPVNLLGPNHHYAHHDQTALQLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZBW4</accession>
    <name>A0AA38ZBW4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glutamyl-tRNA(Gln) amidotransferase subunit A, chloroplastic/mitochondrial</fullName>
            <shortName evidence="7">Glu-AdT subunit A</shortName>
            <ecNumber evidence="7">6.3.5.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">GATA</name>
        <name evidence="9" type="ORF">PVL29_015093</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in chloroplasts and mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>L-glutamyl-tRNA(Gln) + L-glutamine + ATP + H2O = L-glutaminyl-tRNA(Gln) + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.7"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Subunit of the heterotrimeric GatCAB amidotransferase (AdT) complex, composed of A, B and C subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Plastid</location>
            <location evidence="7">Chloroplast stroma</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="7">This protein may be expected to contain an N-terminal transit peptide but none has been predicted.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the amidase family. GatA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.7" evidence="7"/>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZBW4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009570">
        <property type="term" value="C:chloroplast stroma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030956">
        <property type="term" value="C:glutamyl-tRNA(Gln) amidotransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070681">
        <property type="term" value="P:glutaminyl-tRNAGln biosynthesis via transamidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00120">
        <property type="entry name" value="GatA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000120">
        <property type="entry name" value="Amidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020556">
        <property type="entry name" value="Amidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004412">
        <property type="entry name" value="GatA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00132">
        <property type="entry name" value="gatA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895:SF7">
        <property type="entry name" value="GLUTAMYL-TRNA(GLN) AMIDOTRANSFERASE SUBUNIT A, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895">
        <property type="entry name" value="TRANSAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00571">
        <property type="entry name" value="AMIDASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="2 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0934">Plastid</keyword>
    <keyword evidence="5 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="8">
        <location>
            <begin position="70"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="123"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="198"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="7">
        <location>
            <position position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9686042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="546" mass="58242" checksum="F2D950606E69F46D" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZD60</accession>
    <name>A0AA38ZD60_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Wound-responsive family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_015615</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZD60"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022251">
        <property type="entry name" value="DUF3774_wound-induced"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33090">
        <property type="entry name" value="DUF3774 DOMAIN PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12609">
        <property type="entry name" value="DUF3774"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9686841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="149" mass="16651" checksum="2AD658980786B136" modified="2024-01-24" version="1">MSHMGKAWMVAASVGAVEALKDQGFCRWNYTMRSFSRAKKLSSSSSAMVSSRVRDEKAKQSEESLRTVMYLSSSVGAVEALKDQGFCRWNYTMRSIHQHARNSLGSFSQVKKLSSSSAAMVSSRVRDEKAKQSEESLRTVMYLSCWGPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZFJ0</accession>
    <name>A0AA38ZFJ0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF3511 domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_014042</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZFJ0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021899">
        <property type="entry name" value="DUF3511"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33193">
        <property type="entry name" value="DOMAIN PROTEIN, PUTATIVE (DUF3511)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33193:SF71">
        <property type="entry name" value="OS02G0223700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12023">
        <property type="entry name" value="DUF3511"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9688096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="126" mass="14258" checksum="C8728322D3FFE2ED" modified="2024-01-24" version="1">MEDFRSKSCGNGRMQMESYHGGGGGPTSTGMQDLRCYSASYAHPPQTQMGNDPKFKKGKSTNGSLSKAWSFNDPELQRKKRVASYKVYAVEGKMKGSLKKSFRWLKDRYTRVIHGLLWLPLIFISL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZGN6</accession>
    <name>A0AA38ZGN6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BSD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_014399</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZGN6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005607">
        <property type="entry name" value="BSD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035925">
        <property type="entry name" value="BSD_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31923">
        <property type="entry name" value="BSD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31923:SF9">
        <property type="entry name" value="BSD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03909">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00751">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140383">
        <property type="entry name" value="BSD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50858">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BSD" evidence="2">
        <location>
            <begin position="148"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="355"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="58"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="232"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="258"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="271"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9688728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="390" mass="42893" checksum="51DF7F5DFFC8C856" modified="2024-01-24" version="1">MSWLPNPFESPQYDAVSNPSHVAGVREDLSVLSQTIGRQLRGVAAFLAPPPSPPSSPSPVAAPSDSSSSASSSPTLRGIRNDLAAIGGSFKSGLKTFSNLFQSQPGDADDECLEDYDGVAGITEEVLHFVTQLSTRPECWTDFPSALDDDFFMSDIQREHASTIERLAPSFADLRLQLCSYMSDGQFWMIYFILLLPRLNEHDSKLLSTPKIVEARDVLLLKLQNKRDAKVSSENSMVDLSEGSSKVSRTLGEDSPSQEKQVLSESVSVAHEMDKGEQESTERLLEEEDADTGNSVGAVKRLENEDDVSFSDLEDDDNNLSRRLSGLRLKHDIRVSSPNGSNEWVKLNENCEIHGQQRTGQSTSRERDSEGEESNDWLTVDFDSDSLGAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZI77</accession>
    <name>A0AA38ZI77_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Core Histone H2A/H2B/H3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_014791</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the histone H3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9689284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZI77"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22911">
        <property type="entry name" value="HFD_H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000085">
        <property type="entry name" value="Histone H3.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000164">
        <property type="entry name" value="Histone_H3/CENP-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11426">
        <property type="entry name" value="HISTONE H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00622">
        <property type="entry name" value="HISTONEH3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00428">
        <property type="entry name" value="H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="9">
        <location>
            <begin position="38"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9689284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="112" mass="12486" checksum="CB54F93B36FC1C62" modified="2024-01-24" version="1">MEPLIHAASDIQILNSAKAAKKSAPTIGGVKKPHRYSPGTVTLREIRKYQKSIELLICKLPFQRLVLEIAQDFKTGLRFQNHVVLALQEAAEAYLVGLFEDTNLCAIHAHTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZIY5</accession>
    <name>A0AA38ZIY5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Subtilisin-like protease SBT2.4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_012459</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9 10">Belongs to the peptidase S8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9689792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZIY5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02120">
        <property type="entry name" value="PA_subtilisin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04852">
        <property type="entry name" value="Peptidases_S8_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034197">
        <property type="entry name" value="Peptidases_S8_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010259">
        <property type="entry name" value="S8pro/Inhibitor_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045051">
        <property type="entry name" value="SBT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041469">
        <property type="entry name" value="Subtilisin-like_FN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10795">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17766">
        <property type="entry name" value="fn3_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05922">
        <property type="entry name" value="Inhibitor_I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 9 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 9 10" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9 10" id="KW-0720">Serine protease</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041323870" description="Subtilisin-like protease SBT2.4" evidence="11">
        <location>
            <begin position="29"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="domain" description="Inhibitor I9" evidence="13">
        <location>
            <begin position="69"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="12">
        <location>
            <begin position="157"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="domain" description="Subtilisin-like protease fibronectin type-III" evidence="14">
        <location>
            <begin position="723"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8 9">
        <location>
            <position position="166"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8 9">
        <location>
            <position position="241"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8 9">
        <location>
            <position position="606"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF05922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF17766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9689792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="822" mass="87194" checksum="A6A7E17131A1A586" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZJ13</accession>
    <name>A0AA38ZJ13_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein PYRICULARIA ORYZAE RESISTANCE 21-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_012576</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9689976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZJ13"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47005">
        <property type="entry name" value="HEAVY METAL TRANSPORT/DETOXIFICATION SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47005:SF5">
        <property type="entry name" value="HEAVY METAL TRANSPORT_DETOXIFICATION SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9689976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="184" mass="20200" checksum="FCBDD20511B546DF" modified="2024-01-24" version="1">MEEVTEMKLTVNLGCKRCYKKIKKLLCKFPEIRDQTFNEKEDTVIIKVVCCYPEKIRTKLICKGGDCIKCIEIIPPPKPKPPPTPPPKQQPPPPPPPPPPPPPPPPPPPPPPPPPPVTGYPIFIDLTGVCCKSCYGGHGGCTCHRGYVKLVPSYDGWASGCRCDRSYGCRCGLVIEENPTCSIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZJR5</accession>
    <name>A0AA38ZJR5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PVL29_012664</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17 23">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 23">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZJR5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23509">
        <property type="entry name" value="Gnk2-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000195">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001019">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase B120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.430.20">
        <property type="entry name" value="Gnk2 domain, C-X8-C-X2-C motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002902">
        <property type="entry name" value="GNK2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038408">
        <property type="entry name" value="GNK2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002:SF804">
        <property type="entry name" value="OS02G0710500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002">
        <property type="entry name" value="RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD1-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01657">
        <property type="entry name" value="Stress-antifung"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51473">
        <property type="entry name" value="GNK2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 18" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041453840" description="non-specific serine/threonine protein kinase" evidence="18">
        <location>
            <begin position="28"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="20">
        <location>
            <begin position="30"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="20">
        <location>
            <begin position="134"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="326"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAJ9690124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="492" mass="55419" checksum="53117E4534DAAF46" modified="2024-01-24" version="1">MFDTQDTRAFLLLLISTFLLTISSINGQPSYNHCDCSGPDNDSDSANYIDNLSSLLRSLSSKAFTNSFYNDTFNQIYSLYLCRGDVDATTCQNCVKTASQEIQKQCQSNKTAIIWYDECIVRYSNLNFFGIGQTTPMVLMWNAQNTSSREEPPWEAVGMISELLANAPYAENMFNTSEKKLDSGYRYGLVQCSRDMGRDECRNCLTQLRENISQCCNGRRGWQIMCPSCYLRYEDHQFYNHKTLAPSPPPPPPPPSLPPSLVVTALQGPPEDDQEEGTGVEILLNDLEGTTGTCCMEAHMHAKDQDHSREMHYFNFTAILAATNSFSDENKLGEGGFGPVYKGKLLNGKEVAVKRFWAKSGQGHGEFENEVMLLVKLQHKNLVRLLGYCTEEDEKLLVYEYMANTSLDSFLFDPTKSRQLDWAKRTAIVGGIARGLLYLHEDSRLKIIHRDLKASNILLDEEMNPKISDFGTARIFGRNQIDANTSRVVGTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZNY7</accession>
    <name>A0AA38ZNY7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_011507</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Probable 2-oxoglutarate-dependent dioxygenase that may be involved in glucosinolates biosynthesis. May play a role in the production of aliphatic glucosinolates.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZNY7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.330:FF:000022">
        <property type="entry name" value="Probable 2-oxoglutarate-dependent dioxygenase AOP1.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050231">
        <property type="entry name" value="Iron_ascorbate_oxido_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47990">
        <property type="entry name" value="2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00682">
        <property type="entry name" value="IPNSYNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="5 7" id="KW-0408">Iron</keyword>
    <keyword evidence="2 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="8">
        <location>
            <begin position="162"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00057022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9692477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="314" mass="35869" checksum="0A264328C01086FF" modified="2024-01-24" version="1">MGSETPLRLPVIDFSDPELKPGTLQWNLVKAQVQKALEEYGCFEAFFNKVPLELRKAIFGALEELFDLPLQTKLRNSSKKPFHGYVGQYPMVPLYESMGIDDALVQEKAEHFTKLLWPEGNASFCKTIQSFSEQLSELDQTIRRMVLESLGMEKYLDEHMDSTNYLLRVMKYKGPQTTETKLGLNAHTDKNIVTILYQNQVDGLGVQTKDGQWINIKCSPDSFIIMIGDSFLAWTNGRLHSPYHRVMMTGNESRYSTGLFSIPKAGYVIKAPEELVDEEHPLLFKPFDHVEFLHFYYSEAGQRAESALKTYCGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZQ07</accession>
    <name>A0AA38ZQ07_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_011648</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZQ07"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33564:SF15">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33564">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9692667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="132" mass="15083" checksum="CA13488B938F3640" modified="2024-01-24" version="1">MSSILCSQGVVLATAMAVSGILFLAFSRQKSLPSPEFSGIPNSQAPKQILRSCLSNDEKKRERKKKRVQFAENVKEPSGNGKEFRKEQRKKFSRVQRPCRNEIPQIQKMPENQAVLYNGILRDRVHRMACSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZQ60</accession>
    <name>A0AA38ZQ60_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_011866</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZQ60"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9692944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="146" mass="16482" checksum="2A4FC3063C606FB5" modified="2024-01-24" version="1">MVCCLFQNVSPICWRLGEKVITNCAAKLRPYLMEVAKRFSEDAVCPGEVGAFGDESLKSTICNDASETKDDTLTAGNPLNRLDQHSTKKLLVETTDKPVKIPQKRSWKPYFLVNPEEGYDYCWIGRRKRGNPRKIITEASSIQTNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZUN6</accession>
    <name>A0AA38ZUN6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_010234</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZUN6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16454">
        <property type="entry name" value="RING-H2_PA-TM-RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15710">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE PRAJA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15710:SF77">
        <property type="entry name" value="RING-H2 FINGER PROTEIN ATL21B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="185"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9694664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="235" mass="27337" checksum="8B7A9945E4C24F57" modified="2024-01-24" version="1">MSWLRDGYRICKIFLGDNEEAVHWRVIPVHVWRMETSRQMVQAQGNDDGLIASIINKEQHLLRKHTVNVRHQELILDDPHDSIIYQMLRLQRIPHAYSQLLICKVASLARSMIMPNVRPIFIVIMNIVTYVSRPLMELIIEEADNEILATAVTTSMEDETIEIVPATKSSIDKLEKVKMKLDDRCSICLEEFASGMEEVARMPCSHVYHTHCIVKWLEYNHLCPLCRFEMPKLVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZVD3</accession>
    <name>A0AA38ZVD3_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_008324</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZVD3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9696006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="110" mass="12928" checksum="22BCD81B895FCA93" modified="2024-01-24" version="1">MVTLRLTFCGFFFFFFFVFCVLCLCVFLDININMLVWLGVQIREADISRLSFAVELAEEFYGRLLNFNYVTVFCRLVKLFSNIASLCTIFFIAITNLFHIVSSNVMWQIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZVW3</accession>
    <name>A0AA38ZVW3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Time for coffee</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_008441</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042752">
        <property type="term" value="P:regulation of circadian rhythm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039317">
        <property type="entry name" value="TIC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34798">
        <property type="entry name" value="PROTEIN TIME FOR COFFEE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34798:SF1">
        <property type="entry name" value="TIC-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="284"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="382"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="481"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="542"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="718"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="789"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="888"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="943"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1006"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1098"/>
            <end position="1123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1156"/>
            <end position="1177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1190"/>
            <end position="1218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1238"/>
            <end position="1364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="146"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="190"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="255"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="284"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="325"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="390"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="418"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="542"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="569"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="718"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="797"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="888"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="943"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1156"/>
            <end position="1171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1200"/>
            <end position="1218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1238"/>
            <end position="1258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1262"/>
            <end position="1274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1275"/>
            <end position="1303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1326"/>
            <end position="1342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9696196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1364" mass="147032" checksum="BC1D0337DC951C06" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZX06</accession>
    <name>A0AA38ZX06_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_008871</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZX06"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33592:SF17">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING N-TERMINAL PLANT-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33592">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041408458" evidence="1">
        <location>
            <begin position="23"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9696870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="117" mass="12611" checksum="7AAD51EB87B61281" modified="2024-01-24" version="1">MGFTSRKLGLFVVLFSFTLLSAWSFRELEAMRLLGGGPWAKRNSLVLQSLQKGPVLPSGPNPCTHIPEQRAGRCTLNEKNIAGDIATAPPAFSAYMLAQDAAMASLAKDGYDQDHSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZXG0</accession>
    <name>A0AA38ZXG0_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_008682</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZXG0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051724">
        <property type="term" value="F:NAD transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000081">
        <property type="entry name" value="NAD+ transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000105">
        <property type="entry name" value="Nicotinamide adenine dinucleotide transporter 1, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044712">
        <property type="entry name" value="SLC25A32-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45683">
        <property type="entry name" value="MITOCHONDRIAL NICOTINAMIDE ADENINE DINUCLEOTIDE TRANSPORTER 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="8">
        <location>
            <begin position="11"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="8">
        <location>
            <begin position="113"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="8">
        <location>
            <begin position="213"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9696582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="315" mass="34004" checksum="8E987EBC9D64A9B5" modified="2024-01-24" version="1">MPAESHAPSPKVLLCNAGAGAAAGVIAATFVCPLDVIKTRFQVHGLPQLGNGNIKGSLIVGSLEQIFRKEGLRGMYRGLSPTVLALLPNWAVYFTIYEQLKSFLCSNDENHQLSIGANMIAACGAGAATTIATNPLWVVKTRLQTQGMRAGVVPYSSTLSALRRIAYEEGIRGLYSGLVPALAGISHVAIQFPTYEKIKMYLASRENTTMDKLGAPAVAVASSVSKIFASTLTYPHEVVRSRLQEQGHHSEKRYSGVVDCIKKVLQQEGLAGFYRGCATNLLRTTPAAVITFTSFEMIHRFLVNLLPPDPHPHTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZYP7</accession>
    <name>A0AA38ZYP7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Acyl-activating enzyme 18, peroxisomal</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_007066</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9697770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZYP7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44378">
        <property type="entry name" value="ACYL-ACTIVATING ENZYME 17, PEROXISOMAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44378:SF1">
        <property type="entry name" value="ACYL-ACTIVATING ENZYME 18, PEROXISOMAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="242"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="2">
        <location>
            <begin position="189"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="3">
        <location>
            <begin position="638"/>
            <end position="707"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9697770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="727" mass="80586" checksum="DCF66C84EC6F2F81" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZYX0</accession>
    <name>A0AA38ZYX0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pectin acetylesterase</fullName>
            <ecNumber evidence="6">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_008824</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 6">Secreted</location>
            <location evidence="2 6">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the pectinacetylesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="6"/>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZYX0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009505">
        <property type="term" value="C:plant-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052793">
        <property type="term" value="F:pectin acetylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004963">
        <property type="entry name" value="PAE/NOTUM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21562">
        <property type="entry name" value="NOTUM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21562:SF98">
        <property type="entry name" value="PECTIN ACETYLESTERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03283">
        <property type="entry name" value="PAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0134">Cell wall</keyword>
    <keyword evidence="5 6" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041482895" description="Pectin acetylesterase" evidence="6">
        <location>
            <begin position="25"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9696809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="408" mass="44848" checksum="BA5B8D98C85AE34D" modified="2024-01-24" version="1">MSVMAVGVVAVAWCLFFVNGHVLGEDGFQGLLDLEDIPSPNLLMVGLTLIPGAAAKGAVCLDGTLPGYHFHPGFGSGANSWLIQLEGGGWCNSIRSCVFRKTTRRGSSKYMEKTLAFTGILSNKAEENPDFFNWNRVKLRYCDGASFSGEGQNKANGLYFRGQRIFEAGMEELMSKGMKNADQALLSGCSAGGLASIVHCDEFGGLFPKTTKVKCLSDAGLFLDATDVAGGHTMRNLYAGVVSLQGVEKNLPSTCTNRLDPTSCFFPENLIDNIKTPLFLLNAAYDAWQFQESLVPSSVDPHGEWTACKLNHSNCNSTQIQFLQDFRMEMLDDVKGFARYDQNGLFINSCFAHCQTERQDTWFADDSPLIGKKKIAESVGDWYFDRRPVKAIDCPYPCDTTCHNLVFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A3X9</accession>
    <name>A0AA39A3X9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RCK N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_006032</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the castor/pollux (TC 1.A.1.23) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A3X9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044849">
        <property type="entry name" value="CASTOR/POLLUX/SYM8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010420">
        <property type="entry name" value="CASTOR/POLLUX/SYM8_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036721">
        <property type="entry name" value="RCK_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31563:SF13">
        <property type="entry name" value="ION CHANNEL POLLUX-LIKE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31563">
        <property type="entry name" value="ION CHANNEL POLLUX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06241">
        <property type="entry name" value="Castor_Poll_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116726">
        <property type="entry name" value="TrkA C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="181"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="237"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="8">
        <location>
            <begin position="282"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="8">
        <location>
            <begin position="586"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9700521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="848" mass="95348" checksum="0B652792D44ECABE" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A509</accession>
    <name>A0AA39A509_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_006273</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A509"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9700864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="114" mass="11715" checksum="25F30B6A836AF4A5" modified="2024-01-24" version="1">MSNPQQQWTPAQAPIQAQAGAEGVTQSSVGSGNSAQSIRESSQQGDQPQGFLEQTAGQVMDLARGAGKTVMNVAEGAGETVMNVAQGTGQTVMSAAHGTRETVKKSLGADDRRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A5W5</accession>
    <name>A0AA39A5W5_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_006679</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A5W5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012881">
        <property type="entry name" value="DUF1685"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31865:SF0">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31865">
        <property type="entry name" value="OSJNBA0071G03.3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07939">
        <property type="entry name" value="DUF1685"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="137"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9701431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="185" mass="20511" checksum="D166EEA4CD5CF64A" modified="2024-01-24" version="1">MGRLSSETEPMVSPSVFAIETADPDAKTLEKRLETLEIEEQGQWPCGLSTTNSGSKKKKKQKKKKQVLLEGYVETADEDDLMRTKSLTDEDLDELKGCLDLGFGFSYEEIPELCNTLPALELCYSMSQKFMDDQQKAPESSPAASESPGPIANWKISSPGDHPEEVKARLKFWAQAVACTVRLCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A6K4</accession>
    <name>A0AA39A6K4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RBR-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="6">2.3.2.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_006681</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Might act as an E3 ubiquitin-protein ligase, or as part of E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the RBR family. Ariadne subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="6"/>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A6K4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20346">
        <property type="entry name" value="BRcat_RBR_ANKIB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22583">
        <property type="entry name" value="Rcat_RBR_ARI7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1750:FF:000005">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000019">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045840">
        <property type="entry name" value="Ariadne"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048962">
        <property type="entry name" value="ARIH1-like_UBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19422">
        <property type="entry name" value="Ariadne"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22191">
        <property type="entry name" value="IBR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21235">
        <property type="entry name" value="UBA_ARI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="131"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="15">
        <location>
            <begin position="135"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="522"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="26"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="524"/>
            <end position="538"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9701435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="587" mass="66642" checksum="D919F49696E47AE5" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A779</accession>
    <name>A0AA39A779_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">PGG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_006494</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A779"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026961">
        <property type="entry name" value="PGG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24186:SF53">
        <property type="entry name" value="PGG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24186">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13962">
        <property type="entry name" value="PGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="541"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="577"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="609"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="647"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="158"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="241"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="312"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="PGG" evidence="10">
        <location>
            <begin position="496"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="475"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9701173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="671" mass="75018" checksum="0F1F7A44E19E0E77" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ABZ0</accession>
    <name>A0AA39ABZ0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Oxidoreductase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_005089</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ABZ0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019180">
        <property type="entry name" value="Oxidoreductase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039251">
        <property type="entry name" value="OXLD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21193">
        <property type="entry name" value="OXIDOREDUCTASE-LIKE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21193:SF3">
        <property type="entry name" value="OXIDOREDUCTASE-LIKE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09791">
        <property type="entry name" value="Oxidored-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxidoreductase-like" evidence="2">
        <location>
            <begin position="103"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9703618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="146" mass="16891" checksum="13A60BBF67768A33" modified="2024-01-24" version="1">METALPHHPHLLRRRPGVYISTLPNSQFQNIRNDVVLSMRDLALSRISARRFFAHRFRDPKALCIRIPDQMADELSKNQPLEYMENSNKKGEQQPEKSSSPPPPLPEKPLPGDCCGSGCVRCVWDVYYEELEAYNEYYKNESKPKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ADB9</accession>
    <name>A0AA39ADB9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SWIM-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_003456</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ADB9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018289">
        <property type="entry name" value="MULE_transposase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004332">
        <property type="entry name" value="Transposase_MuDR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006564">
        <property type="entry name" value="Znf_PMZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31973">
        <property type="entry name" value="POLYPROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31973:SF129">
        <property type="entry name" value="SWIM-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03108">
        <property type="entry name" value="DBD_Tnp_Mut"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10551">
        <property type="entry name" value="MULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04434">
        <property type="entry name" value="SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00575">
        <property type="entry name" value="ZnF_PMZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="5">
        <location>
            <begin position="439"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9705421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="583" mass="66473" checksum="56ACEEFECBBDA10A" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ADY7</accession>
    <name>A0AA39ADY7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cyclin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_003367</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclin family. Cyclin D subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ADY7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20543">
        <property type="entry name" value="CYCLIN_AtCycD-like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000034">
        <property type="entry name" value="D2/4-type cyclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000040">
        <property type="entry name" value="D6-type cyclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004367">
        <property type="entry name" value="Cyclin_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048258">
        <property type="entry name" value="Cyclins_cyclin-box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02984">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01332">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00292">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3 5" id="KW-0195">Cyclin</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="7">
        <location>
            <begin position="91"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin C-terminal" evidence="8">
        <location>
            <begin position="188"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="317"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9705275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="354" mass="39392" checksum="28F89FE0A9D22701" modified="2024-01-24" version="1">MAQSPDHFSAATAAASLYCAEDAEDVVSWDSDTWISDPGSSPLVYAHSPPFDESTIERLFGSEPDHRPMEDYLCQCRDRSVDVTSRQDSINWILKVHAYYHFRPVTAILSVNYLDRFLSRHALPQGNGWPFQLLSVACLSLAAKMEEPRVPLLLDLQMFQTKFVFEPKTIQRMELWVMANLNWRLRSVTPFDFIDYFASQLPCSSASRHDLLTRVFSVSADLILNTTRVVDFLGFPPSVIAAAAVISASGKRVDFPAGGDWTPESFYERVDREVVRSCHQLMEEYLIDTCPSAPLKDLRSEPPAPASPVGVLDAAACGSCDTRSENPGLSEANPPQPPPPNKRPRFSAPGVQQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AEU2</accession>
    <name>A0AA39AEU2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Molybdate-anion transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_001710</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AEU2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015098">
        <property type="term" value="F:molybdate ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17487">
        <property type="entry name" value="MFS_MFSD5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000478">
        <property type="entry name" value="Molybdate-anion transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008509">
        <property type="entry name" value="MOT2/MFSD5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23516:SF16">
        <property type="entry name" value="MOLYBDATE-ANION TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23516">
        <property type="entry name" value="SAM (S-ADENOSYL METHIONINE) TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05631">
        <property type="entry name" value="MFS_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="129"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="170"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="201"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="285"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="318"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="348"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="413"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9706320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="459" mass="50581" checksum="079AC99BC8D9C1A7" modified="2024-01-24" version="1">MEVFYFVVFGGLAAVVAALELSKSSKDRINTSTAFTSFKNNYLLVYSLMMAGDWLQGPYVYYLYSQYGFGKGDIGQLFIAGFGSSMLFGTIVGSLADKQGRKRACVTYCITYILSCITKHSPQYKILMLGRILGGIATSLLFSAFESWLVAEHNKRGFDQQWLSLTFSKAIFLGNGLIAILSGLFGNVLVDTLGLGPVAPFDAAACFLAIGMAIILSSWTENYGDPSESKDLLTQFKGAAVAIASDEKIALLGAIQSLFEGSMYTFVFLWTPALSPNEEEIPHGFIFATFMLASMLGSSIASRLMARSSTRVESYMQIVFTISSASLLLPIVTNFLVAPSDVKGGSISFKGCIQLLGFCTFEACVGIFWPSIMKMRSQYIPEEARSTIMNFFRIPLNIFVCIVLYNVNAFPITVMFGMCSIFLFVATLLQRRLMVISESQRSRPQDWKERDPEAESLNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AFL7</accession>
    <name>A0AA39AFL7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">rRNA biogenesis protein RRP36</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_001899</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the 90S pre-ribosome involved in the maturation of rRNAs. Required for early cleavages of the pre-RNAs in the 40S ribosomal subunit maturation pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Associates with 90S and pre-40S pre-ribosomal particles.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
            <location evidence="1 6">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the RRP36 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AFL7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009292">
        <property type="entry name" value="RRP36"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21738">
        <property type="entry name" value="RIBOSOMAL RNA PROCESSING PROTEIN 36 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21738:SF0">
        <property type="entry name" value="RIBOSOMAL RNA PROCESSING PROTEIN 36 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06102">
        <property type="entry name" value="RRP36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 6" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4 6" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="213"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="167"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="220"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9706652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="236" mass="27622" checksum="83C40793A6F04DFD" modified="2024-01-24" version="1">MKDAKAAVDHLEQADSSSSEEEEEIERELADVTFEELQKARSDGSHSMHRMPKSEKKGGRKNKNRPMEVSSKKPVSRFRETIQVPKRVVRDPRFESLCGTLDADGFRKRYNFLYETELPAEREGLKKELKKAKDPKVIEELKNQISWIDKQLKSGSVKHTDSKILAEHKKKEREAAKQGKQPFYLKKSEIRKQRNIEKYNSLKASGKLESFIEKRRRRNASKDHRYMPYRRSGDSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AH10</accession>
    <name>A0AA39AH10_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NmrA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_002451</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NmrA-type oxidoreductase family. Isoflavone reductase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AH10"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010283">
        <property type="term" value="F:pinoresinol reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044550">
        <property type="term" value="P:secondary metabolite biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008030">
        <property type="entry name" value="NmrA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050608">
        <property type="entry name" value="NmrA-type/Isoflavone_red_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43349:SF4">
        <property type="entry name" value="PINORESINOL REDUCTASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43349">
        <property type="entry name" value="PINORESINOL REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05368">
        <property type="entry name" value="NmrA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NmrA-like" evidence="2">
        <location>
            <begin position="2"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="NmrA-like" evidence="2">
        <location>
            <begin position="142"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9707432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="275" mass="31190" checksum="B62F16CA6373747B" modified="2024-01-24" version="1">MVKASLDQGRPTFVLQRPEIGLDIEKLQMLLSFKAKGATLVEGSFADHKSLVEAVKKVDVVICTMSGVHFRSHNILLQLKLVEAIKEAGNIHIRFLPSEFGMDPARMGDALEPGRVTFDEKMIVRKAIEEVNIPHTYLAALTPPKEKVCLYGDGNVKAVFVDEYDIATYTIKTIDDPRTLNKTVYMRPPENILSQRQIVEIWEKLTGKKLDKFSISKEDFLASMEGKDFTFQVGVGHFYHIYYDGCLTKFKIGEEGEEAATLYPEVNYKRMDDYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AHZ1</accession>
    <name>A0AA39AHZ1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_002422</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AHZ1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010019">
        <property type="term" value="P:chloroplast-nucleus signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031930">
        <property type="term" value="P:mitochondria-nucleus signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47936:SF1">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN GUN1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47936">
        <property type="entry name" value="PPR_LONG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12854">
        <property type="entry name" value="PPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="9"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9707397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="100" mass="11669" checksum="C4B6E7269B7DEB54" modified="2024-01-24" version="1">MDRRGGCKSLCSYSIYMDVQCKGGRPWRSVKLYKEMKKYGIRLDVGVDFSIRVYREMKELGCEPNVVTYDTIIKLLCEHGRLEKPKGCSIKCVRRIVPRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AIA9</accession>
    <name>A0AA39AIA9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_000286</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AIA9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36367">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36367:SF2">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="180"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="218"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="269"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="301"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9708150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="327" mass="37495" checksum="5E2FE690FC548512" modified="2024-01-24" version="1">MTMASSALLCNNVCTRFWVSSLSQTRYQMQLHQRRRPSVNFHTQHNASLRFAPLVCRASPREEKEVVVEGDDGMRRLLQILLWGAEGIYIFWLFLLPYAPGDPVWAISSYTINSLIGLSLNFFFILPLMNSVGIHLIESPVLHPMSEGLFNFVIGWTFMFAPLLFTDYKRDRYKGSLDVLWGFQMFLTNTFLIPYMAIRLNDEDAEYIPRKPSQLGSVMIHGAPIVGLIGGAICLISALWAFFGRQDSNFGSIADRLEFLVSYLGSERLAYAFIWDICLYIIFQPWLIGDNLQNVQKSKVSFVRYLRFVPVIGLIAYLLFLNLDEEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AIF3</accession>
    <name>A0AA39AIF3_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_000296</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AIF3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34539">
        <property type="entry name" value="T6J4.11 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34539:SF19">
        <property type="entry name" value="T6J4.11 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9708167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="192" mass="21301" checksum="D79FCA22368B14F5" modified="2024-01-24" version="1">MEDYVSKKRVRDDSDEFESDSPEVKRLRDDLLCILDDSDPLADKDPATHDLASVMKIFEEQISASPPRPVPVVDLTSDSGESQPDISFLLEASDDELGLPPSWSSSSEELKSEDAKFVQISTESSGLGEFWGFGDEIPSYDSFEFGFGETHTVANNNGEYVALEGLFDHSDISFGSTDFSEFSWRHETLPAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AK69</accession>
    <name>A0AA39AK69_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">S-adenosylmethionine-dependent methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_000542</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AK69"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000103">
        <property type="entry name" value="SABATH methyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.270">
        <property type="entry name" value="Methyltransferase, alpha-helical capping domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005299">
        <property type="entry name" value="MeTrfase_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042086">
        <property type="entry name" value="MeTrfase_capping"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31009">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE:CARBOXYL METHYLTRANSFERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03492">
        <property type="entry name" value="Methyltransf_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9708552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="350" mass="39294" checksum="6F9DB3EFCAD769FA" modified="2024-01-24" version="1">MQQSFPMNGGDGPHSYLNNSHFQRQAINVSRTMIEEAIAKKLDVKCFSSNPFRLADLGCSVGPNTFIAMQHIVEAVERKYLAQGLKSEMPEFQVFFNDHVANDFNTLFASLPTERRYFACGVPGSFHGRLFPESSIHFMFSSHALHWLSKVPEELLDKNSPAWNRGRIHYTSGPEKVSHAYAAQFEHDMEIFLSARAKELVVGGMIVLLIPALPNGIPISQSPFGAMFDLLGASLMDMAKEGLISEAEVDSFNLPIHIASPEQMTELVERNECLIIERMEFVDPRSNLFGLINGKECAMFLRASLEGVFAQYFESGIIDQLFDRLSKKIMESSHQLEPVGTLLFVVLRLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D5C8</accession>
    <name>A0AA39D5C8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Enhancer of mRNA-decapping protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_025931</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">P-body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat EDC4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031087">
        <property type="term" value="P:deadenylation-independent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.100:FF:000001">
        <property type="entry name" value="Enhancer of mRNA-decapping protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000232">
        <property type="entry name" value="enhancer of mRNA-decapping protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.100">
        <property type="entry name" value="conserved c-terminal region of ge- 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045152">
        <property type="entry name" value="EDC4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049404">
        <property type="entry name" value="EDC4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044938">
        <property type="entry name" value="EDC4_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032401">
        <property type="entry name" value="EDC4_WD40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15598">
        <property type="entry name" value="ENHANCER OF MRNA-DECAPPING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15598:SF5">
        <property type="entry name" value="ENHANCER OF MRNA-DECAPPING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21289">
        <property type="entry name" value="EDC4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16529">
        <property type="entry name" value="Ge1_WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 9" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Enhancer of mRNA-decapping protein 4 WD40 repeat region" evidence="11">
        <location>
            <begin position="214"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="261"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="380"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="Enhancer of mRNA-decapping protein 4 C-terminal" evidence="12">
        <location>
            <begin position="1271"/>
            <end position="1377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="626"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="700"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="740"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="916"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="37"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="97"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="626"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="747"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="920"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="942"/>
            <end position="968"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF21289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9672518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1402" mass="151555" checksum="6CA873B03B891FF8" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D796</accession>
    <name>A0AA39D796_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Receptor-like serine/threonine-protein kinase</fullName>
            <ecNumber evidence="17">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">PVL29_025502</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="23">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16 25">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 25">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="17"/>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D796"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048544">
        <property type="term" value="P:recognition of pollen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00028">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01098">
        <property type="entry name" value="PAN_AP_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000060">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.90.10.10:FF:000004">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000195">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.4.10:FF:000002">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.10">
        <property type="entry name" value="Bulb-type lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.4.10">
        <property type="entry name" value="Hepatocyte Growth Factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021820">
        <property type="entry name" value="S-locus_recpt_kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000858">
        <property type="entry name" value="S_locus_glycoprot_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024171">
        <property type="entry name" value="SRK-like_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32444">
        <property type="entry name" value="BULB-TYPE LECTIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32444:SF98">
        <property type="entry name" value="RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11883">
        <property type="entry name" value="DUF3403"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08276">
        <property type="entry name" value="PAN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00954">
        <property type="entry name" value="S_locus_glycop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000641">
        <property type="entry name" value="SRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00473">
        <property type="entry name" value="PAN_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 17" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 19" id="KW-0732">Signal</keyword>
    <keyword evidence="4 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041273963" description="Receptor-like serine/threonine-protein kinase" evidence="19">
        <location>
            <begin position="23"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="438"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="21">
        <location>
            <begin position="23"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="22">
        <location>
            <begin position="338"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
            <begin position="509"/>
            <end position="786"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRNR" id="PIRNR000641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAJ9671827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="827" mass="92907" checksum="4E52168CEC242964" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D808</accession>
    <name>A0AA39D808_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Amino-acid permease BAT1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_023231</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D808"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004840">
        <property type="entry name" value="Amino_acid_permease_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649:SF17">
        <property type="entry name" value="AMINO ACID PERMEASE_ SLC12A DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649">
        <property type="entry name" value="AMINO-ACID PERMEASE BAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00218">
        <property type="entry name" value="AMINO_ACID_PERMEASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="68"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="113"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="155"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="185"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="238"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="276"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="335"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="385"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="410"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="455"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="482"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9673540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="522" mass="57557" checksum="E123A44EC0545D39" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D8L2</accession>
    <name>A0AA39D8L2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AT-hook motif nuclear-localized protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_023058</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Transcription factor that specifically binds AT-rich DNA sequences related to the nuclear matrix attachment regions (MARs).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D8L2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003680">
        <property type="term" value="F:minor groove of adenine-thymine-rich DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010228">
        <property type="term" value="P:vegetative to reproductive phase transition of meristem"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11378">
        <property type="entry name" value="DUF296"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.80:FF:000002">
        <property type="entry name" value="AT-hook motif nuclear-localized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.80">
        <property type="entry name" value="Hypothetical protein, similar to alpha- acetolactate decarboxylase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014476">
        <property type="entry name" value="AHL15-29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005175">
        <property type="entry name" value="PPC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31100">
        <property type="entry name" value="AT-HOOK MOTIF NUCLEAR-LOCALIZED PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31100:SF51">
        <property type="entry name" value="AT-HOOK MOTIF NUCLEAR-LOCALIZED PROTEIN 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03479">
        <property type="entry name" value="PCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016021">
        <property type="entry name" value="ESCAROLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117856">
        <property type="entry name" value="AF0104/ALDC/Ptd012-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51742">
        <property type="entry name" value="PPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="PPC" evidence="8">
        <location>
            <begin position="96"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="227"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="25"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="45"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="58"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="241"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR016021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9673222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="292" mass="29741" checksum="C1FDF3D3F37BE879" modified="2024-01-24" version="1">MAGYEPGSGSRYVHQLLGPELHLQRPSSLPQHQATQQPSDSKDESPDDQEQREDTEEAAAASSGGATTSSNRRPRGRPPGSRNKPKPPIIVTRDSPNALRSHVLEVAAGADIMESVLNYARRRGRGVCVLSGGGTVMNVTLRQPASPAGSIVTLHGRFEILSLSGTVLPPPAPPSAGGLSIFLSGGQGQVVGGNVVGPLMASGPVVLMAASFANAVFERLPLEEEEGAVQVQPTASQSSGVTGGGGGGQLGDGGGSSGGGGVPIYNMGANMGNFPFPGDLLRWGGSAPRPPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D959</accession>
    <name>A0AA39D959_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_023986</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D959"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11393">
        <property type="entry name" value="bHLH_AtbHLH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000032">
        <property type="entry name" value="Transcription factor bHLH123 family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045239">
        <property type="entry name" value="bHLH95_bHLH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045843">
        <property type="entry name" value="IND-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16223:SF56">
        <property type="entry name" value="TRANSCRIPTION FACTOR BHLH110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16223">
        <property type="entry name" value="TRANSCRIPTION FACTOR BHLH83-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="8">
        <location>
            <begin position="303"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="284"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="370"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9674785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="430" mass="46493" checksum="1C03E95BD262B243" modified="2024-01-24" version="1">MESANRHHQHQLQDQLVVSSPLLAANPSCYAPAPSNHAWTPNIILNSGSFNPNFNGILFNPRDSRQKNDSILHPLNSSVVQDLGFHWASNAGSFTSQSAHDLHPKIKEELSESFPKFTEMINSSSSAVEDLHLPPTSYIKSKDLNDLSEKLLLKSFSSGCQINGLQLSAGEFYANAQSCNTGFGGVAIPSRGHFSQIFPTINISNLSQSSSTISSSLDMNLQALDLLTSARFSGTFSQPSHNNLGLFKDSLSFGLDHLQQSTNRPSNSSSKISPFTNGVAEVKRPSSFLEPKATQATPKKSRLESRASCPPIKVRKEKLGDRIAALQQLVAPFGKTDTASVLMEAIGYIKFLQNQVETLSVPYMKSSRNRSSISMQGVRKGSADGEGSEEPRRDLRSRGLCLVPLSCMSYVTTDCGGGGVWPPPSFGGGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DA47</accession>
    <name>A0AA39DA47_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RecQ-mediated genome instability protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">BLM-associated protein of 75 kDa homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_021881</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RMI1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9676586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DA47"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016604">
        <property type="term" value="C:nuclear body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031422">
        <property type="term" value="C:RecQ family helicase-topoisomerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.770:FF:000004">
        <property type="entry name" value="RecQ-mediated instability protein (DUF1767)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.770">
        <property type="entry name" value="RecQ-mediated genome instability protein Rmi1, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032199">
        <property type="entry name" value="RMI1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049363">
        <property type="entry name" value="RMI1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042470">
        <property type="entry name" value="RMI1_N_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013894">
        <property type="entry name" value="RMI1_OB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14790:SF15">
        <property type="entry name" value="RECQ-MEDIATED GENOME INSTABILITY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14790">
        <property type="entry name" value="RECQ-MEDIATED GENOME INSTABILITY PROTEIN 1 RMI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16099">
        <property type="entry name" value="RMI1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08585">
        <property type="entry name" value="RMI1_N_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21000">
        <property type="entry name" value="RMI1_N_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01161">
        <property type="entry name" value="DUF1767"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RMI1 N-terminal" evidence="7">
        <location>
            <begin position="108"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="RecQ mediated genome instability protein 1 OB-fold" evidence="5">
        <location>
            <begin position="158"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="RecQ-mediated genome instability protein 1 C-terminal OB-fold" evidence="6">
        <location>
            <begin position="464"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="22"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="35"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="72"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00077519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF16099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF21000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9676586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="627" mass="67623" checksum="C7EE52898A6E90E7" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DB62</accession>
    <name>A0AA39DB62_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CTLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_024695</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DB62"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd12885">
        <property type="entry name" value="SPRY_RanBP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013144">
        <property type="entry name" value="CRA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024964">
        <property type="entry name" value="CTLH/CRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006595">
        <property type="entry name" value="CTLH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044736">
        <property type="entry name" value="Gid1/RanBPM/SPLA_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050618">
        <property type="entry name" value="Ubq-SigPath_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12864">
        <property type="entry name" value="RAN BINDING PROTEIN 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10607">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00757">
        <property type="entry name" value="CRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00668">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50897">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="165"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="CTLH" evidence="3">
        <location>
            <begin position="232"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9675862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="403" mass="45518" checksum="EACEBFDBF5F4C01D" modified="2024-01-24" version="1">MLNDNSQDLATHFLKLSRLGSTRSAMDVDDDDDDQEEPPTELNTINSSGGFLLVSPDKLSVKYPTVHMHGHDVGVVQGNRPAPVKRLVYYFEIHVKSAGVKGQIAIGFTCEGFKMRRQPGKNGAVVGTTRKDVKGRLYPTVAVHSQNEEVTVNFGADPFCFDLKVFIYIYIYMFFFFTLFLFSLFFRVVRSYLLHYGYEDTLNSFDLAGKSNVPPISLDQGSGSNEEGRMYALSQRKVLRQLIRKGKIDAALGKLGEWYPDIVQDDKSATCFLLYCQKFIELVRVGELEEAVSYGRTKLAKFFELPGFEELVQDCVALLAYEQPHKSVVGYLLEDSQREVVADTVNAMILLRNPKVTDTQVCLRSDLEKLLRQLTACCLMKRQLEGDQGEAFHLHRVLNSSDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DB79</accession>
    <name>A0AA39DB79_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NmrA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_022197</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DB79"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05259">
        <property type="entry name" value="PCBER_SDR_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008030">
        <property type="entry name" value="NmrA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050608">
        <property type="entry name" value="NmrA-type/Isoflavone_red_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045312">
        <property type="entry name" value="PCBER-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43349:SF89">
        <property type="entry name" value="LEUCANTHOCYANIDIN REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43349">
        <property type="entry name" value="PINORESINOL REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05368">
        <property type="entry name" value="NmrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NmrA-like" evidence="4">
        <location>
            <begin position="24"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9677080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="362" mass="39039" checksum="21AE441CC007B2FD" modified="2024-01-24" version="1">MTVLSASTAPPPQAPPAATGPRTLVVGASGFIGRFVAEASLSSGHPTYVLVRSSATTSPSKANTIKSLEDQGAILVTGSIGDKEVMIEAMRKYEIEVVISAVGGATILDQLTLAEAIKAVGSIKRFLPSEFGHDIDRAEPVEPGLTMYMEKRKVRRFIEEAAIPYTYICCNSIAAWPYHDNTHPADVLPPLDRFHIYGDGSVKAYFVAGTDIGKFTIKTINDDRTVNKSVHFRPPNNRVSINELASLWEKKIGRTLPRVTVEEDDLLAAAAEMCIPESVVASFTHDIFIKGCQVNFSLDKPTDLEATSLYPEMQFRTIDECFDEFVEKIMGGQAAAEKAAGKEGIGVPTSAPDALVITATCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DBI9</accession>
    <name>A0AA39DBI9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">xyloglucan 6-xylosyltransferase</fullName>
            <ecNumber evidence="12">2.4.2.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_022453</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>Transfers an alpha-D-xylosyl residue from UDP-D-xylose to a glucose residue in xyloglucan, forming an alpha-(1-&gt;6)-D-xylosyl-D-glucose linkage.</text>
            <dbReference type="EC" id="2.4.2.39"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 34 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.39" evidence="12"/>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DBI9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033843">
        <property type="term" value="F:xyloglucan 6-xylosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009969">
        <property type="term" value="P:xyloglucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000032">
        <property type="entry name" value="xyloglucan 6-xylosyltransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008630">
        <property type="entry name" value="Glyco_trans_34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31311:SF13">
        <property type="entry name" value="XYLOGLUCAN 6-XYLOSYLTRANSFERASE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31311">
        <property type="entry name" value="XYLOGLUCAN 6-XYLOSYLTRANSFERASE 5-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05637">
        <property type="entry name" value="Glyco_transf_34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="95"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00051628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00066326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9677470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="450" mass="51243" checksum="1761B7C23A78ED01" modified="2024-01-24" version="1">MGLDNLTTQKRTGAGIPTTNGRPAGGRLSGLPRGRQIHKTFNNIKITILCGFVTILVLRGTIGVGNLGGSGGEVENQNLIEETNRILAEIRSDGDPSDPDDPAESEINPNVTYTLGPKITNWNEERKVWLDRNPEFPNFVNGKARILLVTGSPPNPCDNPIGDHYLLKSTKNKIDYCRIHGIEIVYNMAHLDKELAGYWAKLPLIRRLMLSHPEVEWIWWMDSDALFTDMVFEIPLSKYDNYNLVVHGYPDLMFNQKSWIALNTGSFLFRNCQWSLDLLDAWAPMGPKGPIRDEAGKILTANLKGRPAFEADDQSALIYLLISKKDEWMDKVFLENSYYLHGYWAGLVDRYEEMIEKYHPGLGDERWPFVTHFVGCKPCGSYGDYPVERCLRSMERAFNFADNQVLKLYGFRHRGLLSPKIKRIRNETATPLEFVDQFDIRRPEHGGHSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DBS9</accession>
    <name>A0AA39DBS9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein bL12 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_022628</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DBS9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00387">
        <property type="entry name" value="Ribosomal_L7_L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1390.10:FF:000001">
        <property type="entry name" value="50S ribosomal protein L7/L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000206">
        <property type="entry name" value="Ribosomal_bL12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013823">
        <property type="entry name" value="Ribosomal_bL12_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014719">
        <property type="entry name" value="Ribosomal_bL12_C/ClpS-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45987">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45987:SF6">
        <property type="entry name" value="RIBOSOMAL PROTEIN L12_ ATP-DEPENDENT CLP PROTEASE ADAPTOR PROTEIN CLPS FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00542">
        <property type="entry name" value="Ribosomal_L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54736">
        <property type="entry name" value="ClpS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein bL12 C-terminal" evidence="4">
        <location>
            <begin position="126"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9677755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="193" mass="21364" checksum="3385AE2800C85CE0" modified="2024-01-24" version="1">MKFTALVKTARVHPTLLKIIGPLQYRFFQHDFVPREPNAKPKRYKYPPFYDPYGPKPPPSEKVVELAERIAALAPEERRQIGPMLKDRLSLPKTQVISTEGMELGSQGGAAAGAGKVEEKVEKTAFDVKLEKFEAAAKIKVIKEVRAFTSLGLKEAKDLVEKVPVLIKQGITKEEANDIIEKIKAAGGVAVME</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DC93</accession>
    <name>A0AA39DC93_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">beta-aspartyl-peptidase</fullName>
            <ecNumber evidence="4">3.4.19.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_020859</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of a beta-linked Asp residue from the N-terminus of a polypeptide.</text>
            <dbReference type="EC" id="3.4.19.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterotetramer of two alpha and two beta chains arranged as a dimer of alpha/beta heterodimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Ntn-hydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.5" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DC93"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008798">
        <property type="term" value="F:beta-aspartyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003948">
        <property type="term" value="F:N4-(beta-N-acetylglucosaminyl)-L-asparaginase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04513">
        <property type="entry name" value="Glycosylasparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.30:FF:000003">
        <property type="entry name" value="N(4)-(Beta-N-acetylglucosaminyl)-L-asparaginase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.30">
        <property type="entry name" value="(Glycosyl)asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000246">
        <property type="entry name" value="Peptidase_T2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10188">
        <property type="entry name" value="L-ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10188:SF6">
        <property type="entry name" value="N(4)-(BETA-N-ACETYLGLUCOSAMINYL)-L-ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01112">
        <property type="entry name" value="Asparaginase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041373686" description="beta-aspartyl-peptidase" evidence="11">
        <location>
            <begin position="22"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="220"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="248"/>
            <end position="251"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="271"/>
            <end position="274"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Cleavage; by autolysis" evidence="10">
        <location>
            <begin position="219"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR600246-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR600246-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR600246-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9678791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="355" mass="37682" checksum="17252FC2F605C628" modified="2024-01-24" version="1">MAVRNLLITLLLFTLFSMALGHEVGNLGHYPVVVSTWPFVEAVRAAWRAIDSGFSAVDAVVEGCSACEELRCDGTVGPGGSPDENGETTLDALVMNGATMEVGAVAAMRYVKDGIKAARLVMQHTGHTLLVGEQASAFAISMGLPGPTNLSSSESVQKWSKWKENRCQPNFWKNIVPVDSCGPYHPKGIAELGKGTCSSANLMAAAESRSSHIGLHNHDTISMAVIDKEGHIAVGTSTNGATYKIPGRVGDGPIAGSSSYADDEVGACGATGDGDIMMRFLPCYQVVESMRLGTEPKLAAQDAIKRIARKFPDFVGAVFAINKNGLHAGACHGWTFQYSVRSPEMEDVEVFTVFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DD95</accession>
    <name>A0AA39DD95_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GDSL esterase/lipase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_022786</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9678007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DD95"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22835:SF557">
        <property type="entry name" value="LIPASE_HYDROLASE FAMILY PROTEIN, PUTATIVE, EXPRESSED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22835">
        <property type="entry name" value="ZINC FINGER FYVE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041348616" description="GDSL esterase/lipase" evidence="5">
        <location>
            <begin position="30"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9678007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="384" mass="41738" checksum="4AC4E8A7FD5C6642" modified="2024-01-24" version="1">MASSFKLFSSLSSLTTILILILLSSLCTASTAPTGTNSPPFKRIYAFGDSYTDTGNTESATGPNAFTYVSHLPYGSTFFHHPTNRYSDGRLVIDFVAQALSLPFLPPYRNQKANTSTGVNFAVAGSTAIPHEFFVKNNLTLDITPQSIQTQLVWFNKFLEKQGCGGATINSGCTFDDTLFWVGEIGANDYAYTVGSSVPGSTIQELGIKSITSFLQALLKKGVKYLVVQGLPPTGCLTLALTLAPDDDRDATGCVGSVNKQSYSHNTILQAKLHDLRVQFPHAVIVYADYWNAYHTIMKNGDRYGFKEPFKTCCGSGGDPYNFDVFATCGSSSASACPNPSQYINWDGVHLTEAMYKVVANSFLHGGFCHPSFDYLLSRKQHLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DEX8</accession>
    <name>A0AA39DEX8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ADF-H domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_020409</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the actin-binding proteins ADF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9681521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DEX8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030042">
        <property type="term" value="P:actin filament depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11286">
        <property type="entry name" value="ADF_cofilin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000025">
        <property type="entry name" value="Actin-depolymerizing factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002108">
        <property type="entry name" value="ADF-H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017904">
        <property type="entry name" value="ADF/Cofilin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11913">
        <property type="entry name" value="COFILIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00241">
        <property type="entry name" value="Cofilin_ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00102">
        <property type="entry name" value="ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51263">
        <property type="entry name" value="ADF_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ADF-H" evidence="3">
        <location>
            <begin position="5"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9681521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="139" mass="16105" checksum="5C33D3B2F4A0F755" modified="2024-01-24" version="1">MANSASGMAVHDECKLKFLELKAKRNHRFIVFKIDERIQQVMVEKLGSPDETYEDFTNSLPANECRYAVYDFDFTTDENCQKSKIFFIAWSPDTSRVRSKMLYASSKDRFKRELDGIQVELQATDPSEMSMDIIKGRAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DGL6</accession>
    <name>A0AA39DGL6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">L-3-cyanoalanine synthase</fullName>
            <ecNumber evidence="3">4.4.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_020344</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>hydrogen cyanide + L-cysteine = 3-cyano-L-alanine + hydrogen sulfide + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17821"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18407"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:77860"/>
            <dbReference type="EC" id="4.4.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cysteine synthase/cystathionine beta-synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.4.1.9" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9681427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DGL6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004124">
        <property type="term" value="F:cysteine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050017">
        <property type="term" value="F:L-3-cyanoalanine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019499">
        <property type="term" value="P:cyanide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01561">
        <property type="entry name" value="CBS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000002">
        <property type="entry name" value="Cysteine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000006">
        <property type="entry name" value="Cysteine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050214">
        <property type="entry name" value="Cys_Synth/Cystath_Beta-Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001216">
        <property type="entry name" value="P-phosphate_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10314">
        <property type="entry name" value="CYSTATHIONINE BETA-SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00901">
        <property type="entry name" value="CYS_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0198">Cysteine biosynthesis</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="9">
        <location>
            <begin position="56"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00050896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9681427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="389" mass="42569" checksum="D3781E8AAAEE1C5B" modified="2024-01-24" version="1">MAALSSFFKRKSSIPFTSLAIRRWFSTQPILDSPSFSQRIRDLPKNLPGTNIKKEVSQLIGKTPLVFLNKVTEGCGAYVAVKQEMMQPTASIKDRPALAMITDAENKQLITPGKTVLIEPTSGNMGISMAFMAAMKGYKMVLTMPSYTSLERRVTMRAFGADLILTDPTKGMGGTVKKAYELLESTPDAFMLQQFSNPANTQVHFETTGPEIWEDTHGQVDIFIMGIGSGGTVSGVGRYLKSQNPNVKIYGLEPTESNVLNGGKPGPHHITGNGVGFKPDILDMDVMEEVLMLQQSFPISLDRVSNFMIQVSSEDAVNMARQLALKEGLMVGISSGANTVAALRLARRPENKGKLIVTVHPSFGERYLSSVLFQELRKEAENMQPVSVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DHA3</accession>
    <name>A0AA39DHA3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">PGG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_017032</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DHA3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025314">
        <property type="entry name" value="DUF4219"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026961">
        <property type="entry name" value="PGG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24177:SF329">
        <property type="entry name" value="ANKYRIN REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24177">
        <property type="entry name" value="CASKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13961">
        <property type="entry name" value="DUF4219"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13962">
        <property type="entry name" value="PGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="592"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="624"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="668"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="701"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4219" evidence="4">
        <location>
            <begin position="19"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="167"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase Ty1/copia-type" evidence="3">
        <location>
            <begin position="363"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="PGG" evidence="5">
        <location>
            <begin position="580"/>
            <end position="691"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9684848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="746" mass="83497" checksum="7430BB5C2ED75DC4" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DHF2</accession>
    <name>A0AA39DHF2_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_017145</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DHF2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34213">
        <property type="entry name" value="NUCLEAR TRANSPORT FACTOR 2 (NTF2) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34213:SF2">
        <property type="entry name" value="NUCLEAR TRANSPORT FACTOR 2 (NTF2) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9684998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="187" mass="21594" checksum="0C455B45F1D79BC3" modified="2024-01-24" version="1">MEKNYQDKQEMDQQREQLGKEASEVNALRRVSDDIIPHILNLYGACAAPRDFEIYAPDATFEDPLMCAHGVKQIKSAFYSLSKVFNESKIVEYSVKENLISPEKTEILMDNKQHYKFLGRGIDMISLIKLHVEGGKVVRHEDWWDKKPLLNRETAKLPLVGRIVEMTRRGSMLVTHVMMRFGKDPTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DHL1</accession>
    <name>A0AA39DHL1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cucumisin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_016734</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the peptidase S8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DHL1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02120">
        <property type="entry name" value="PA_subtilisin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04852">
        <property type="entry name" value="Peptidases_S8_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000006">
        <property type="entry name" value="Subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2310">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034197">
        <property type="entry name" value="Peptidases_S8_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010259">
        <property type="entry name" value="S8pro/Inhibitor_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045051">
        <property type="entry name" value="SBT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041469">
        <property type="entry name" value="Subtilisin-like_FN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10795">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17766">
        <property type="entry name" value="fn3_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05922">
        <property type="entry name" value="Inhibitor_I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 7" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Peptidase S8/S53" evidence="8">
        <location>
            <begin position="134"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="Subtilisin-like protease fibronectin type-III" evidence="10">
        <location>
            <begin position="279"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="Inhibitor I9" evidence="9">
        <location>
            <begin position="420"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="8">
        <location>
            <begin position="516"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="domain" description="Subtilisin-like protease fibronectin type-III" evidence="10">
        <location>
            <begin position="1021"/>
            <end position="1117"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6 7">
        <location>
            <position position="524"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6 7">
        <location>
            <position position="588"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6 7">
        <location>
            <position position="915"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9684416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1125" mass="118325" checksum="4DFB4BB578F3DD74" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DLR4</accession>
    <name>A0AA39DLR4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Bicarbonate transporter-like transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_014294</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the anion exchanger (TC 2.A.31.3) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DLR4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005452">
        <property type="term" value="F:solute:inorganic anion antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006820">
        <property type="term" value="P:monoatomic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050801">
        <property type="term" value="P:monoatomic ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.570:FF:000004">
        <property type="entry name" value="probable boron transporter 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.570">
        <property type="entry name" value="Helical hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011531">
        <property type="entry name" value="HCO3_transpt-like_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003020">
        <property type="entry name" value="HCO3_transpt_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453">
        <property type="entry name" value="ANION EXCHANGE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453:SF131">
        <property type="entry name" value="BORON TRANSPORTER 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00955">
        <property type="entry name" value="HCO3_cotransp"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0039">Anion exchange</keyword>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="90"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="121"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="196"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="229"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="292"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="331"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="542"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="domain" description="Bicarbonate transporter-like transmembrane" evidence="9">
        <location>
            <begin position="7"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="Bicarbonate transporter-like transmembrane" evidence="9">
        <location>
            <begin position="200"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="Bicarbonate transporter-like transmembrane" evidence="9">
        <location>
            <begin position="449"/>
            <end position="591"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9688569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="667" mass="75470" checksum="4AB69F05065ADA95" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DLT1</accession>
    <name>A0AA39DLT1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA/RNA-binding protein Alba-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_014325</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DLT1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.110.20:FF:000005">
        <property type="entry name" value="Uncharacterized protein At2g34160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.20">
        <property type="entry name" value="Alba-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036882">
        <property type="entry name" value="Alba-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002775">
        <property type="entry name" value="DNA/RNA-bd_Alba-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014560">
        <property type="entry name" value="UCP030333_Alba"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31947">
        <property type="entry name" value="DNA/RNA-BINDING PROTEIN ALBA 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31947:SF36">
        <property type="entry name" value="DNA_RNA-BINDING PROTEIN ALBA-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01918">
        <property type="entry name" value="Alba"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF030333">
        <property type="entry name" value="UCP030333_Alba"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82704">
        <property type="entry name" value="AlbA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA/RNA-binding protein Alba-like" evidence="1">
        <location>
            <begin position="20"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9688614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="130" mass="14638" checksum="9D3660D6A186756C" modified="2024-01-24" version="1">MEEITEGVNNMNIADSHKKNRIQVSNTKKPLFFYVNLAKRYMQQHNEVELSALGMAIATVVTIAEILKNNGLAVEKKIMTSTVDMKDESRGRPIQKAKIEILLGKTENFDELMAAAAEEREVAEVEEQQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DPA4</accession>
    <name>A0AA39DPA4_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="25">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">PVL29_013411</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="25">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Serine/threonine-protein kinase that may function as a signaling receptor of extracellular matrix component. Binding to pectin may have significance in the control of cell expansion, morphogenesis and development.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="19">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="25">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DPA4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000038">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000084">
        <property type="entry name" value="Wall-associated receptor kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000628">
        <property type="entry name" value="Wall-associated receptor kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000043">
        <property type="entry name" value="Wall-associated receptor kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045274">
        <property type="entry name" value="WAK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025287">
        <property type="entry name" value="WAK_GUB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27005">
        <property type="entry name" value="WALL-ASSOCIATED RECEPTOR KINASE-LIKE 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27005:SF353">
        <property type="entry name" value="WALL-ASSOCIATED RECEPTOR KINASE-LIKE 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13947">
        <property type="entry name" value="GUB_WAK_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 19" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="13 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7 22" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041323739" evidence="22">
        <location>
            <begin position="30"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="360"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="24">
        <location>
            <begin position="306"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="440"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="469"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00058961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="KAJ9691224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="761" mass="85165" checksum="E7A5A105BF910DAC" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DPY1</accession>
    <name>A0AA39DPY1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Checkpoint protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_011688</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the HUS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DPY1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030896">
        <property type="term" value="C:checkpoint clamp complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044778">
        <property type="term" value="P:meiotic DNA integrity checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033314">
        <property type="term" value="P:mitotic DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031573">
        <property type="term" value="P:mitotic intra-S DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.70.10.10:FF:000010">
        <property type="entry name" value="Checkpoint protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.70.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016580">
        <property type="entry name" value="HUS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007150">
        <property type="entry name" value="HUS1/Mec3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12900:SF0">
        <property type="entry name" value="CHECKPOINT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12900">
        <property type="entry name" value="MITOTIC AND DNA DAMAGE CHECKPOINT PROTEIN HUS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04005">
        <property type="entry name" value="Hus1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF011312">
        <property type="entry name" value="Cell_cycle_HUS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR011312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9692723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="323" mass="36001" checksum="CBE82C65201381CB" modified="2024-01-24" version="1">MKFKAFLTDNGTSLLEKRFLPALDKMGKVCHLYFTRDHTIFLHNLLNGDGVQSIAQFRKEALFDNYRISSQNDDRIAFTIDLSLLQRAIRSSVSIYAEMGGGDGVDTGSNRLQIKLVKKLPPNSHQPLPFLTFESKGYKSAVIQDVPISKPLSRADVLELQSALDMAQDLPRTLVQVPDLNQLQNYVDRMKHVGDLLNISISKYGDLHVQISTTLITLGAEFRKLLVVGEQVEVPAEDRDLSAQSRTQRAVQRGDAQTVQVSMKHFAKSLQCHLAKPDCAFYGIAQEGACLTVIFQFFIPGSRQNDKSISLHCRLPVLDPGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DRI5</accession>
    <name>A0AA39DRI5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutathione S-transferase</fullName>
            <ecNumber evidence="1">2.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_011967</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Is involved in the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GST superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="1"/>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9693055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DRI5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045073">
        <property type="entry name" value="Omega/Tau-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11260">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE, GST, SUPERFAMILY, GST DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11260:SF764">
        <property type="entry name" value="GLUTATHIONE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="2">
        <location>
            <begin position="3"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU369102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9693055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="61" mass="7232" checksum="62EA7AC6C3FC1924" modified="2024-01-24" version="1">MADEIILLDFWPSMFGMRVRVALAEKGLKYEYRQEDLRNKSPLLLEMNPVRKKIPVLVLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DST4</accession>
    <name>A0AA39DST4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Receptor-like serine/threonine-protein kinase</fullName>
            <ecNumber evidence="17">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="ORF">PVL29_010335</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="26">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16 28">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 28">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="26">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="17"/>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DST4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048544">
        <property type="term" value="P:recognition of pollen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00028">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00053">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01098">
        <property type="entry name" value="PAN_AP_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000059">
        <property type="entry name" value="S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000302">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.90.10.10:FF:000007">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.90.10.10:FF:000015">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.4.10:FF:000022">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.10">
        <property type="entry name" value="Bulb-type lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.4.10">
        <property type="entry name" value="Hepatocyte Growth Factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000858">
        <property type="entry name" value="S_locus_glycoprot_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024171">
        <property type="entry name" value="SRK-like_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47974">
        <property type="entry name" value="OS07G0415500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47974:SF4">
        <property type="entry name" value="RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00954">
        <property type="entry name" value="S_locus_glycop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000641">
        <property type="entry name" value="SRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00473">
        <property type="entry name" value="PAN_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 17 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 18" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 17 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 17" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 21" id="KW-0732">Signal</keyword>
    <keyword evidence="4 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041417016" description="Receptor-like serine/threonine-protein kinase" evidence="21">
        <location>
            <begin position="24"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="452"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="24">
        <location>
            <begin position="20"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="23">
        <location>
            <begin position="282"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="25">
        <location>
            <begin position="332"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="511"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="540"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRNR" id="PIRNR000641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EMBL" id="KAJ9694809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="803" mass="90557" checksum="1ACA19E74C6F9B98" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DU25</accession>
    <name>A0AA39DU25_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DDE Tnp4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_008393</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HARBI1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DU25"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035098">
        <property type="term" value="C:ESC/E(Z) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035102">
        <property type="term" value="C:PRC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045249">
        <property type="entry name" value="HARBI1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930:SF85">
        <property type="entry name" value="GH03217P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE Tnp4" evidence="9">
        <location>
            <begin position="179"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9696129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="385" mass="43134" checksum="D4D366214263002C" modified="2024-01-24" version="1">MAPPKKSKKAKRESKKSKKCKSMSVGPIEPRATESDWWDSFWQKNSPIPGSAIPTDEAEGFKFFFRVSKKTFDYICSLVRQDLVSRPPSGLINIQGRLLSVEKQVAIALRRLASGESQVSVGAAFGVGQSTVSQVTWRFIEAVEERAKHHLRWPDFNRMEEIKSKFETSYGLSNCCGAIDASHIIMTLPTVQTSDDWCDQENNYSMFLQGVVDDEMRFLDIVTGWPGGMTVARLLKCTGFYRNCEAGERLNVNIRILSEGVEIREYVVGGVGYPLLPWLITPCEYNDISSSMSGFKPNHEAARSLAVRAFSQLKGTWRILSKVCCLLHNIIIDSKDKLQPDVALSSHHDPGYGQQCCKQIDPLGKAMRENIAKHLQHGKEKAPSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DU97</accession>
    <name>A0AA39DU97_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_011007</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DU97"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.80">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003441">
        <property type="entry name" value="NAC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036093">
        <property type="entry name" value="NAC_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31719">
        <property type="entry name" value="NAC TRANSCRIPTION FACTOR 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31719:SF94">
        <property type="entry name" value="PROTEIN ATAF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02365">
        <property type="entry name" value="NAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101941">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51005">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NAC" evidence="5">
        <location>
            <begin position="1"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9695815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="161" mass="18388" checksum="05B98520F195E979" modified="2024-01-24" version="1">MELLYVREFLSEKNHNHNAHRENQWYFFTLRERKYPNGTRPSRSVAHGLDGYWKATSVDKAIISDGQVIGFRKALDFYDGNQSSGIKTKWKMHEYRLNQKSLPPNNSNGSDPTKLDDWVLCKVYTKGGSNKSKDHQENAEVKVVATDESGVQATTNLPQAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DUH0</accession>
    <name>A0AA39DUH0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Xyloglucan endotransglucosylase/hydrolase</fullName>
            <ecNumber evidence="7">2.4.1.207</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_009293</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Secreted</location>
            <location evidence="7">Cell wall</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Secreted</location>
            <location evidence="7">Extracellular space</location>
            <location evidence="7">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="7">Contains at least one intrachain disulfide bond essential for its enzymatic activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the glycosyl hydrolase 16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.207" evidence="7"/>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DUH0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016762">
        <property type="term" value="F:xyloglucan:xyloglucosyl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044042">
        <property type="term" value="P:glucan metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044791">
        <property type="entry name" value="Beta-glucanase/XTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010713">
        <property type="entry name" value="XET_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31062">
        <property type="entry name" value="XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASE PROTEIN 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00722">
        <property type="entry name" value="Glyco_hydro_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06955">
        <property type="entry name" value="XET_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0052">Apoplast</keyword>
    <keyword evidence="1 7" id="KW-0134">Cell wall</keyword>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6 7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GH16" evidence="8">
        <location>
            <begin position="1"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9697403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="225" mass="26118" checksum="AFE044656861C30D" modified="2024-01-24" version="1">MSKHTYGSGFFRMRIKIPNKDSLGIITTFYLTSHQNNQPAGNHDELDFEFLGGPGKPYTLQTNVFSNDNGGREERVALWFDPTADFHTYKILWNQHQIVFYVDKIPIRVFENNTGIGVNYPSQAMSVVGSLWSDTTWAAHGQQINWTQAPFVAYFQEFNINGCLEDIDSTSQCNSTHYWWNGPRYLGLDQTEIVNYEKVRRKYLTYSYCSDRGSLHPECRRRTLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DUS2</accession>
    <name>A0AA39DUS2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GPI transamidase component PIG-T</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_008506</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DUS2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042765">
        <property type="term" value="C:GPI-anchor transamidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016255">
        <property type="term" value="P:attachment of GPI anchor to protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007245">
        <property type="entry name" value="PIG-T"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12959:SF11">
        <property type="entry name" value="GPI TRANSAMIDASE COMPONENT PIG-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12959">
        <property type="entry name" value="GPI TRANSAMIDASE COMPONENT PIG-T-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04113">
        <property type="entry name" value="Gpi16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041213790" description="GPI transamidase component PIG-T" evidence="2">
        <location>
            <begin position="24"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="569"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="645"/>
            <end position="662"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9696310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="664" mass="74713" checksum="C4ADD0908540E60E" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DV41</accession>
    <name>A0AA39DV41_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">glucomannan 4-beta-mannosyltransferase</fullName>
            <ecNumber evidence="11">2.4.1.32</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Glucomannan synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_010613</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>GDP-mannose + (glucomannan)n = GDP + (glucomannan)n+1.</text>
            <dbReference type="EC" id="2.4.1.32"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the glycosyltransferase 2 family. Plant cellulose synthase-like A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.32" evidence="11"/>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DV41"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047259">
        <property type="term" value="F:glucomannan 4-beta-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051753">
        <property type="term" value="F:mannan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06437">
        <property type="entry name" value="CESA_CaSu_A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000015">
        <property type="entry name" value="Glucomannan 4-beta-mannosyltransferase 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32044">
        <property type="entry name" value="GLUCOMANNAN 4-BETA-MANNOSYLTRANSFERASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32044:SF77">
        <property type="entry name" value="GLUCOMANNAN 4-BETA-MANNOSYLTRANSFERASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13632">
        <property type="entry name" value="Glyco_trans_2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="36"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="368"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="400"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="481"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="507"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="14">
        <location>
            <begin position="190"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00051800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00060879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00066505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00076024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF13632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9695212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="533" mass="60849" checksum="08BD07FA545C1C27" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DVN7</accession>
    <name>A0AA39DVN7_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_009138</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DVN7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9697225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="32" mass="3810" checksum="A44BE2678D9EEB15" modified="2024-01-24" version="1">MNLRMRFWNLNSEIWGRKTLKEKTVGGMGMSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DWE0</accession>
    <name>A0AA39DWE0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Copper transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_007568</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the copper transporter (Ctr) (TC 1.A.56) family. SLC31A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DWE0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005375">
        <property type="term" value="F:copper ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007274">
        <property type="entry name" value="Cop_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483:SF24">
        <property type="entry name" value="COPPER TRANSPORTER 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 31 COPPER TRANSPORTERS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04145">
        <property type="entry name" value="Ctr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0186">Copper</keyword>
    <keyword evidence="3 6" id="KW-0187">Copper transport</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="83"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9698559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="137" mass="15240" checksum="D8B128F162440D4E" modified="2024-01-24" version="1">MDHDHMHMSPPPANSTGMPPMHHMEMMMHMAFYWGKKGEILFSGWPGTRSGMYALALIVVFVMGIIVEWLSHCQLIKPGGLEYMVMLALMSFNGGVFLVAVAGHAVGFLVFGSRLFRGSETLRPSEEAFYDLPPMSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DXV5</accession>
    <name>A0AA39DXV5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Alkaline/neutral invertase</fullName>
            <ecNumber evidence="6">3.2.1.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_008166</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Invertase that cleaves sucrose into glucose and fructose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.</text>
            <dbReference type="EC" id="3.2.1.26"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the glycosyl hydrolase 100 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.26" evidence="6"/>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9699445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DXV5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033926">
        <property type="term" value="F:endo-alpha-N-acetylgalactosaminidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004575">
        <property type="term" value="F:sucrose alpha-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005987">
        <property type="term" value="P:sucrose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000001">
        <property type="entry name" value="probable alkaline/neutral invertase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024746">
        <property type="entry name" value="Glyco_hydro_100"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31916">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31916:SF36">
        <property type="entry name" value="ALKALINE_NEUTRAL INVERTASE E, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12899">
        <property type="entry name" value="Glyco_hydro_100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5 6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9699445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="639" mass="71597" checksum="B0A6C7E1BBF95942" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DXY7</accession>
    <name>A0AA39DXY7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Hexosyltransferase</fullName>
            <ecNumber evidence="11">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_005819</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Glycan metabolism; pectin biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Endomembrane system</location>
            <topology evidence="10">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the glycosyltransferase 8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="11"/>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DXY7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000024">
        <property type="entry name" value="Hexosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002495">
        <property type="entry name" value="Glyco_trans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050748">
        <property type="entry name" value="Glycosyltrans_8_dom-fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13778">
        <property type="entry name" value="GLYCOSYLTRANSFERASE 8 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13778:SF5">
        <property type="entry name" value="HEXOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01501">
        <property type="entry name" value="Glyco_transf_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041393352" description="Hexosyltransferase" evidence="12">
        <location>
            <begin position="27"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00060399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9700179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="352" mass="39942" checksum="2CE5588AFBAE20F9" modified="2024-01-24" version="1">MAFWSSSLLPLLGLLSLFFLHPFSTSSSVHAIRLGAVVRRPSPDVPIFREAPAFRNGDACGTRDADRIHVSMTLDANYLRGTMAAALSILQHSTCPENLSFHFLSAQHVPEIVSRIQATFPYLNFRVYRFDSNRVRGKISKSIRQALDQPLNYARIYLADILPANVRRVIYLDSDLVMVDDISNLWGVDLGDKVVAAPEYCHANFTKYFTDEFWSSPEMAKTFKGRSPCYFNTGVMVVDVDRWRKGGYTQKVEEWMAVQKQNRIYDLGSLPPFLLVLAGNIKAVDHRWNQHGLGGDNLEGKCRNLHPGPISLLHWSGKGKPWLRLDSRKPCAVDHLWAPYDLYRSSRVSLEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DYC6</accession>
    <name>A0AA39DYC6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">RHOMBOID-like protein</fullName>
            <ecNumber evidence="10">3.4.21.105</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_007496</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Serine protease involved in intramembrane proteolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>Cleaves type-1 transmembrane domains using a catalytic dyad composed of serine and histidine that are contributed by different transmembrane domains.</text>
            <dbReference type="EC" id="3.4.21.105"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Membrane</location>
            <topology evidence="2 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the peptidase S54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.105" evidence="10"/>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DYC6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1540.10:FF:000019">
        <property type="entry name" value="RHOMBOID-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002610">
        <property type="entry name" value="Peptidase_S54_rhomboid-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22936:SF77">
        <property type="entry name" value="RHOMBOID-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22936">
        <property type="entry name" value="RHOMBOID-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 10" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="56"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="138"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="195"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="223"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="248"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="296"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S54 rhomboid" evidence="11">
        <location>
            <begin position="129"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9698437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="391" mass="43271" checksum="7CB9BCB182A7C624" modified="2024-01-24" version="1">MGSDPPSSNIEIKIHPRRQDNVVHPAVPSPIGVPAASAPPRQQQHLFSEFRPFKRWFPWMVPTFVVANIVMFLITMFINNCPKNSVSCVADFLGRFSFQPLKENPLLGPSSSTLEKMGALEVSKVVHRHQVWRLISCIWLHAGVFHVLANMLSLVFIGIRLEQEFGFVRIGLLYVVSGFGGSMLSSLFIQSSISVGASGALFGLLGGMLSELITNWTIYANKFAALLTLILIIIVNLAVGILPHVDNFAHIGGFVSGFLLGFVFLIRPQYGWVSQRNASPGHIAASIKPKHKMYQYVLWVMSLILLTVGFTVGMVMLLRGVNGNDHCSWCHYLSCVPTSKWSCKSQQAYCESTRIGNQLNLTCLSNGRSNIYSLPDDNTSQVQQLCSQLCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E0A7</accession>
    <name>A0AA39E0A7_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_004327</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E0A7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004158">
        <property type="entry name" value="DUF247_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31170:SF25">
        <property type="entry name" value="BNAA09G04570D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31170">
        <property type="entry name" value="BNAC04G53230D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03140">
        <property type="entry name" value="DUF247"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="390"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9702534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="418" mass="48044" checksum="CEBA92E7A7AA14F0" modified="2024-01-24" version="1">MLKNLTPLSTTCCIYRVPQTLQKENREAYTPRVVSIGPVHHGEGHLVAMEEHKLRYLQNFLSKSRKTLADCVEIISREEKRARDCYIESINMSSEEFVKMILLDGCFIVEVILGFSNCHPIKPDDRVYKKPWLFFEVRRDMTLLENQLPFFLLVTLYSLLPLPPGQQNHCCSFLLLSVEFFKDYLQIPEAKSSRDIMIEIVKKTSSTLGEVKHFVDLLILCLQPSSPSPAKPVDFKLVIPTATHLREGGVRFKLHEEDKPLLGIEYSRGVLRIPSLILKEKSESLFRNLIAFEQCHSREHANYITDYIYLMDQLIDTTNDVNLLVRKRIISSHLGDSIAVKNLFNRLLIGAELSRDTFHFSGIYVELSEHLASPWNSWKATLRHDYFCSLWRGASTIAAVILLLLTLLQTIFSAMSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E0M1</accession>
    <name>A0AA39E0M1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_003313</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E0M1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025724">
        <property type="entry name" value="GAG-pre-integrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054722">
        <property type="entry name" value="PolX-like_BBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039537">
        <property type="entry name" value="Retrotran_Ty1/copia-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057670">
        <property type="entry name" value="SH3_retrovirus"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648:SF20">
        <property type="entry name" value="RNA-DIRECTED DNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648">
        <property type="entry name" value="TRANSPOSASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13976">
        <property type="entry name" value="gag_pre-integrs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22936">
        <property type="entry name" value="Pol_BBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25597">
        <property type="entry name" value="SH3_retrovirus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="3">
        <location>
            <begin position="152"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="390"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="392"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9705191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="485" mass="55866" checksum="7946BB71FD7B1949" modified="2024-01-24" version="1">MGDSRSTPVIGKGKVLLKLTFGKVLALCDVLHVPYIRWNLVSVSLLGKAGVRILFDSDKIVLTKNDAFVGKGYYNQGLFLLNVYDIINNNASSFSAYIVDFCDIWYGRLGHVNFSYMKRMVELSLIPKLSLENHGKCESCVESKTIKKFCKSVERESDLLSLIHSDLGDLKNTMTRGGKRFYITFIDDYSRYTRVYLLRNKDEARDVFIKYKNEVENQLSKKIKRLRTDRGREYESNPFNSFCEDHGIINETTPPYSPESNGVAERKNRILKDMMNAMLVSSGAPLNLWGEAILSACHIQNRIPYKKTAYLKVWECLAKVLIPEPKKRKLGLKTFDAMFIGYAENSTTYRFLITKSENNPVDVKTIIETKNAEFFENIFPMKLNGEQQVQKTSRDKSIEPFEFEPKKSKRDRKETNLGDGFYTFLINEDPKSYKEAITSPDAPFWKEAIDSEIESIMYNHTWELVDLPPGAKTIGCKWIFKRKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E119</accession>
    <name>A0AA39E119_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glycosyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_006521</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E119"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080043">
        <property type="term" value="F:quercetin 3-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080044">
        <property type="term" value="F:quercetin 7-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000019">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000167">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926">
        <property type="entry name" value="GLUCOSYL/GLUCURONOSYL TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926:SF870">
        <property type="entry name" value="UDP-GLYCOSYLTRANSFERASE 75B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9701212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="465" mass="50998" checksum="17677F9CE8318D7E" modified="2024-01-24" version="1">MGQHHFLIISHPLQGHINPALQLAKRLIRTGAHVTFAVSVSAHRRMPKGPTLPGLTLVPFSDGYDDGLKSNDHLQHYMSEIKRCGSETLRRITAISTDQGRPVTCLLHTILLTWVAELARSLQVPSALLWIQSATVFTIYYHYFNGYGDVIGDCSSEGSSPIELPGLPILLSSCDIPSFLLSSNIYASALSTFQEEMEALRQETNPKVLVNTFDALEAEALRAVDKVEVMGIGPLVPSAFLDAKDPSDTSFGGDIFQDPSDCIDWLNSKPKSSVVYVSFGTLCVLSKQQMEKIAGALLHSGRPFLWVIRSAPGNGEVEEEKLSCREELEEKGMIVAWCPQLDVLSHPSLGCFITHCGWNSTLECLASGVPVVAFPQWTDQGTNGKLIEDVWKTGVRVTANEEGIVESEEIKRCLEVVMGRGERGEELRRNAGKWKDLAREAVKDGGSSDYNLKAFLDELGQGSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E296</accession>
    <name>A0AA39E296_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_002922</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ARG7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E296"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009733">
        <property type="term" value="P:response to auxin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003676">
        <property type="entry name" value="SAUR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31175">
        <property type="entry name" value="AUXIN-RESPONSIVE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31175:SF82">
        <property type="entry name" value="AUXIN-RESPONSIVE PROTEIN SAUR65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02519">
        <property type="entry name" value="Auxin_inducible"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="63"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9704589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="122" mass="14060" checksum="D8FF19148CE469FE" modified="2024-01-24" version="1">MAKKWQKLTSMTKQISLQRGNEILHILTAAATRFVFPIVYLSNHIFRELFKMSEEEFGLPRDGPIMLPCDAVFMNYVVFLIKRRVTKDMEKALLVSMATSQCSRCHSLCQEESSQHVLLCGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E2I0</accession>
    <name>A0AA39E2I0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Zinc finger LSD1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_002230</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E2I0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040319">
        <property type="entry name" value="LSD1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036280">
        <property type="entry name" value="Multihaem_cyt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005735">
        <property type="entry name" value="Znf_LSD1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01053">
        <property type="entry name" value="LSD1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31747:SF1">
        <property type="entry name" value="PROTEIN LOL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31747">
        <property type="entry name" value="PROTEIN LSD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06943">
        <property type="entry name" value="zf-LSD1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48695">
        <property type="entry name" value="Multiheme cytochromes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zinc finger LSD1-type" evidence="3">
        <location>
            <begin position="28"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="Zinc finger LSD1-type" evidence="3">
        <location>
            <begin position="67"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="Zinc finger LSD1-type" evidence="3">
        <location>
            <begin position="105"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF06943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9707141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="145" mass="15201" checksum="106CD1F8814EFD62" modified="2024-01-24" version="1">MPVPLAPYPTPPVPFTQPANGAQSQLVCSGCRNLLLYPVGATSVCCAVCNVVTAVPPPGTEMAQLVCGGCHTLLMYIRGATSVQCSCCHTINLALEANQVAHVNCGNCRMLLMYQYGARSVKCAVCNFVTSVGASTSSTEHKFNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E2N1</accession>
    <name>A0AA39E2N1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Late embryogenesis abundant protein LEA-2 subgroup domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_001555</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E2N1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055301">
        <property type="entry name" value="Lea14-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004864">
        <property type="entry name" value="LEA_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31852">
        <property type="entry name" value="LATE EMBRYOGENESIS ABUNDANT (LEA) HYDROXYPROLINE-RICH GLYCOPROTEIN FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03168">
        <property type="entry name" value="LEA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117070">
        <property type="entry name" value="LEA14-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="Late embryogenesis abundant protein LEA-2 subgroup" evidence="2">
        <location>
            <begin position="66"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9706043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="183" mass="20172" checksum="16B7CC8DEA494D40" modified="2024-01-24" version="1">MKKEGRKNTKCMAYVAAFIVFQTIIILVFAPTLLKIKSPKLRFGKVDVESFSNSTSSFTMKLQAQVAVKNTNFGHFKYPNNTIPITYGGVTVGEVVVPKGRARARSTNRFTVAVELSFDQLSSNSTLGNDITSGSGLLPLVSQAKLPGKIHLMKVMKKKKTAEMYCTIKVNLMNCVVQDLKCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E3D9</accession>
    <name>A0AA39E3D9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_003641</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E3D9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032567">
        <property type="entry name" value="RTL1-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15503">
        <property type="entry name" value="LDOC1 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15503:SF45">
        <property type="entry name" value="RNA-DIRECTED DNA POLYMERASE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08284">
        <property type="entry name" value="RVP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="107"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="60"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="129"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="61"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="84"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="129"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9705669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="341" mass="39265" checksum="3F7EA0ADC836111A" modified="2024-01-24" version="1">MIVTEYAAKFTQLSRYALNVVANEQILNIRAQVTPFMLRTYSEVVARALVIEREMEEAQRLRSRNSRFGGSKKRERDFKHQKVTHPQQQPRKQDQYSGTTDWAKGPRRCYECGEVGHLRREYPKFQRLTYQPSQRQPQGEGNFRQGKPGGKLEQGQQGRFYAMGPQNAESNALVEGMILCFSTWAHVLFDPGATHSFIFASFASMLDIEFVPLHCSLCVETPMGGKVETKWVCHACVLCIGGLQVTMDLVLLDISSFDVIVGMDWLARHHAVLDCYLKKATFQTSSSSYLSFYGDRRLTFIPLIRNLDDKWSRKDGGRHFLFKYEGRRQKDDNYRLYSNGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E4F6</accession>
    <name>A0AA39E4F6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_000897</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E4F6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000383">
        <property type="entry name" value="Leucine-rich repeat receptor protein kinase EMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000716">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000400">
        <property type="entry name" value="Nuclear pore complex protein NUP107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000299">
        <property type="entry name" value="Piriformospora indica-insensitive protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27000">
        <property type="entry name" value="LEUCINE-RICH REPEAT RECEPTOR-LIKE PROTEIN KINASE FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27000:SF803">
        <property type="entry name" value="RECEPTOR-LIKE PROTEIN 45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 12" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041376448" description="Leucine-rich repeat-containing N-terminal plant-type domain-containing protein" evidence="12">
        <location>
            <begin position="31"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="13">
        <location>
            <begin position="39"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9709151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="639" mass="69992" checksum="1F716D0FE6CD0014" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E5V8</accession>
    <name>A0AA39E5V8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PUB 62/63 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_001144</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E5V8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057649">
        <property type="entry name" value="PUB62-63_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33644:SF3">
        <property type="entry name" value="RING_U-BOX SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33644">
        <property type="entry name" value="U-BOX DOMAIN-CONTAINING PROTEIN 62-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23112">
        <property type="entry name" value="PUB62-63_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PUB 62/63 C-terminal" evidence="2">
        <location>
            <begin position="480"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="16"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9709523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="558" mass="58960" checksum="1AAC6434D9F7B6DD" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E858</accession>
    <name>A0AA39E858_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase M20 dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_000859</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="5">The Mn(2+) ion enhances activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E858"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010179">
        <property type="term" value="F:IAA-Ala conjugate hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009850">
        <property type="term" value="P:auxin metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08017">
        <property type="entry name" value="M20_IAA_Hyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.360:FF:000001">
        <property type="entry name" value="N-acetyldiaminopimelate deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017439">
        <property type="entry name" value="Amidohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044757">
        <property type="entry name" value="ILR1-like_Hyd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01891">
        <property type="entry name" value="amidohydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11014:SF147">
        <property type="entry name" value="PEPTIDASE M20 DIMERISATION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11014">
        <property type="entry name" value="PEPTIDASE M20 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005962">
        <property type="entry name" value="Pept_M20D_amidohydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 5" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041222914" description="Peptidase M20 dimerisation domain-containing protein" evidence="6">
        <location>
            <begin position="18"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="7">
        <location>
            <begin position="222"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="405"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR005962-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9709090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="462" mass="50395" checksum="EA2DBC745180844F" modified="2024-01-24" version="1">MGILQLLLFFLIPVTTPFSCLNLQYSLNSLTVPHRNSSSKEHITGLANDPITVNWMKRIRREIHENPELAYEEFATSALIRRELEQLGIGYRWPIAGTGVVATIGSGSQPFVALRSDMDALPIQEMVEWEHKSKVDGKMHACGHDAHVAMLLGAAKILQEIRDELPGTVVLIFQPAEERGVGAKAMIQEGALENVEAIFGIHAVIEYPTGTVAARSGEFLAGCGGFRAKISGRGGHAAVPQHSIDPILAVSTSVVSLQNIVSRETDPLDHQVVSVAMIHGGTAFNVIPDAATITGTFRAFSKKGFYALRDRIEEVIKAQAAVNRCSAEIDFSGMELPTIPPTINDGRIYEHARKVSSEMVGEENTKISPVCMGSEDFAFYLEKVPGSFLFLGMRNEKAGSTYPPHSPYYVLDEEVLPIGAAIHAAFALSYLSDSNNNSHFHTGLVETYVETEPAEFMRGTKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YHS7</accession>
    <name>A0AA38YHS7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">WW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_026926</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YHS7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WW" evidence="1">
        <location>
            <begin position="53"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9670693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="136" mass="16403" checksum="B31559888E5478AD" modified="2024-01-24" version="1">MRNQLLHLKCLKHKPLKCIHLKCIDHKFTYCKCKYLKRMHLVFLLVFNLDYSPSDWVEHTFARRKYYYNKRTRQSSWVKTPKLMTPIKSRKPENPHNIFGPFDHLLTKEVKVKDMEENEDRPVLTMIIDANVSCNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YJ39</accession>
    <name>A0AA38YJ39_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_025190</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YJ39"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34541">
        <property type="entry name" value="OS01G0729900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34541:SF2">
        <property type="entry name" value="OS01G0729900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9671375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="470" mass="51633" checksum="EC8D42E619001B63" modified="2024-01-24" version="1">MSVERSFEAWEEVQRHGHDLADRLAQFTGLIQSHITPPSFQWPNPQKPKLFDVEFPSQSFGNRDFGIAVDNSGINGVSAIFDIGNRLGQVGAEFGACLNGVVQQFFRRLPVPFRQDEGVEASVRVGGSGQRADLGVALQEDFRLATERLREFGFAENEGTLDGLVEEEIPGFDLSSAGHFGRPQGTINITSTYDSRTRDVESSLVARGDLWRVEASHGSSTSGSENSSLFLVQLGPVLFVRDTTLLLPVHLSKQHLLWYGYDRKNGMHSLCPAVWSKHRRWLLMSMICLNPLACSFMDLQFPNGQFTYVSGEGLTTSAFLPVFGGLLQAQGQYPGEMKFSFSCKNKWGTRITPIVQWPDKSFTLGLAQALAWRRSGLMVRPAIQFSVCPTFGGTNPGLRAELIHSVNEDLSLICGCAYTIHPSAFASISLGRSKWNGNVGSSGIVARVETPLGNFGRPSFSVQLNSGIEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YJ60</accession>
    <name>A0AA38YJ60_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-directed DNA polymerase</fullName>
            <ecNumber evidence="2">2.7.7.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">DNA polymerase PolI-like B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PVL29_025204</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14 20">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA polymerase type-A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YJ60"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006264">
        <property type="term" value="P:mitochondrial DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033259">
        <property type="term" value="P:plastid DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd08640">
        <property type="entry name" value="DNA_pol_A_plastid_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06139">
        <property type="entry name" value="DNA_polA_I_Ecoli_like_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000034">
        <property type="entry name" value="DNA polymerase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000051">
        <property type="entry name" value="DNA polymerase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1060.10">
        <property type="entry name" value="Taq DNA Polymerase, Chain T, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002562">
        <property type="entry name" value="3'-5'_exonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001098">
        <property type="entry name" value="DNA-dir_DNA_pol_A_palm_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002298">
        <property type="entry name" value="DNA_polymerase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10133">
        <property type="entry name" value="DNA POLYMERASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10133:SF27">
        <property type="entry name" value="DNA POLYMERASE NU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00476">
        <property type="entry name" value="DNA_pol_A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01612">
        <property type="entry name" value="DNA_pol_A_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00868">
        <property type="entry name" value="DNAPOLI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00482">
        <property type="entry name" value="POLAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="13" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="9" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="DNA-directed DNA polymerase family A palm" evidence="17">
        <location>
            <begin position="848"/>
            <end position="1078"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="252"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00079253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ9671391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1118" mass="124579" checksum="F87D639D6B5488E2" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YJL5</accession>
    <name>A0AA38YJL5_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_025401</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YJL5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9671674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="147" mass="17324" checksum="FE55479191E050B7" modified="2024-01-24" version="1">MLSSLKIKPLKTWIKLKSQSLSVKNRLIWVQKVVQKEQVDIVERNNESAVDDVEENPTVENDGLKQQQEQATSKLPTKTQLRRSTRERQPSKRYTGDEYLLLSDGGELENYQEVLLYDEKKKWLRAIHEEMKSLHKNNTYELIELPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YJQ7</accession>
    <name>A0AA38YJQ7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription factor TFIIIC triple barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_025416</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YJQ7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000127">
        <property type="term" value="C:transcription factor TFIIIC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006383">
        <property type="term" value="P:transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.4370:FF:000002">
        <property type="entry name" value="Transcription factor TFIIIC, tau55-related protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.4370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042771">
        <property type="entry name" value="GTF3C6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019481">
        <property type="entry name" value="TFIIIC_triple_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21860:SF2">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR 3C POLYPEPTIDE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21860">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIIC TFIIIC , POLYPEPTIDE 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10419">
        <property type="entry name" value="TFIIIC_sub6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription factor TFIIIC triple barrel" evidence="1">
        <location>
            <begin position="13"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9671690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="119" mass="13368" checksum="FEFE652501021712" modified="2024-01-24" version="1">MEENLVHNESLEEEEEEYVLLNLDEVRGQVDIPPNAPYVLSGLDTPNPILVIGDSLKLIGEYEETIGTCLVFSEGRKFIIDPNQAPAKEVKPIASLQKIWKFRLLYEGDVQDATLVSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YN73</accession>
    <name>A0AA38YN73_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tetratricopeptide repeat protein 33</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_023213</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YN73"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052658">
        <property type="entry name" value="TPR-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15544">
        <property type="entry name" value="OSMOSIS RESPONSIVE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15544:SF0">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="129"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="40"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="51"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9673512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="203" mass="22423" checksum="881CE0F66CA4B875" modified="2024-01-24" version="1">MKITWKKQSKGNNRKRSLPALSNLPFEDEEAEQATSTMEAKVEEEGKTLDSSDGPSTQQLADSFQAQGDKLAEGGKYREALGKWETALTLMPGKAVLHEQKAQVLLEIGDAWNALKAASRATELEPSWAEAWITLGRAQLNFGEPDIAIKSFDRALAIKPNSEEAQDDRKSALHLVKRRKLLHSSGLSDTENRFVVSDKTESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YQE1</accession>
    <name>A0AA38YQE1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_023965</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YQE1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9674748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="75" mass="8490" checksum="F54901FE2D3F01D4" modified="2024-01-24" version="1">MELLSFSPCKVSLIAKSASLIRSSREEFWNVSNLCNRGEPLIHPSQLQADQHHRAVSRTFGCKPPCRQAERPVDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YRL1</accession>
    <name>A0AA38YRL1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">GDSL esterase/lipase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_024290</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YRL1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051238">
        <property type="entry name" value="GDSL_esterase/lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45650">
        <property type="entry name" value="GDSL-LIKE LIPASE/ACYLHYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45650:SF2">
        <property type="entry name" value="OS06G0560700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041399793" description="GDSL esterase/lipase" evidence="8">
        <location>
            <begin position="22"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9675272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="372" mass="40271" checksum="E135FA465249CFF6" modified="2024-01-24" version="1">MSPKTHPQNLLCLSCFIYVLASFLLPCSCSTTSSPTDRGDQIKGMFVFGSSLVDTGNNNFLQTTTRADFLPYGIDFPGGPSGRFTNGKNVVDLIGDHLHLPSIPPFSSPATRGAAILRGVDFASGGSGILDNTGSFLGEVTSLNQQIRNFEKVTLPDLEAQLRVKSSESLSSYLFVVGVGGNDITFNYFLHAINRNISLQVFTATMTTLLSAQLKKLYSLGGRKFALMSVNPLGCTPMAIQLPRKVYANRLNQAARLFNFRLKSLIDEMEAEMPGSQLVLVNTYQIINSIIKNPNAKGFKDTTSPCCEVKSSVSGSILCKRGGEACGNRSSYVFFDGLHPTEAVNAIIASRAYDSNDSDLVYPTNIKQLANL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YRL3</accession>
    <name>A0AA38YRL3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Late embryogenesis abundant protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_024351</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YRL3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053325">
        <property type="entry name" value="H3-Acetyl_Activator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35706:SF1">
        <property type="entry name" value="EMBRYOGENESIS-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35706">
        <property type="entry name" value="F14O23.11 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9675375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="177" mass="19908" checksum="4A61E25326893930" modified="2024-01-24" version="1">MNQHTCNSLCKLILRHPFQKSSKPTSLIRLTSLAQIPNQLVSDSVPSPKPQILYTSASKFHYFRPPNGSSLSTQFISRVILRNYSGESASELDKDKVVDEINLKFAEAREEIEMALESKETVYFDEEASCARAAVKEVVDMFDGLLEKLPESDRGAVQRSMGLKIEQLKAEVEQLND</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YRQ2</accession>
    <name>A0AA38YRQ2_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_024302</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YRQ2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36002">
        <property type="entry name" value="PYRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36002:SF1">
        <property type="entry name" value="PYRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9675284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="88" mass="9595" checksum="FDABD49D58CFC2AE" modified="2024-01-24" version="1">MSLACLVCHSVESPSHSFRSYSVSSSDNEGRCSAIANCLARRASLPAPTINSSIIRSGIPGTPMLVRSCAVRRDIVKDWNFEEVVMEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YRX0</accession>
    <name>A0AA38YRX0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_024372</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YRX0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025724">
        <property type="entry name" value="GAG-pre-integrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054722">
        <property type="entry name" value="PolX-like_BBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039537">
        <property type="entry name" value="Retrotran_Ty1/copia-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057670">
        <property type="entry name" value="SH3_retrovirus"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648">
        <property type="entry name" value="TRANSPOSASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648:SF28">
        <property type="entry name" value="TRANSPOSON-ENCODED PROTEIN WITH RIBONUCLEASE H-LIKE AND RETROVIRUS ZINC FINGER-LIKE DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13976">
        <property type="entry name" value="gag_pre-integrs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22936">
        <property type="entry name" value="Pol_BBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25597">
        <property type="entry name" value="SH3_retrovirus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="6">
        <location>
            <begin position="507"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="228"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="247"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9675422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1040" mass="118853" checksum="14920551DC3716BC" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YVT4</accession>
    <name>A0AA38YVT4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_022552</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YVT4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47941">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="135"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="205"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="240"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="275"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="310"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="345"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="380"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="415"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9677626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="492" mass="56515" checksum="62006A599D340247" modified="2024-01-24" version="1">MLILANRLSSLRPTKPLLRPISYNYKLTTTTSPSSPPQDATIVNLVLKTDSQTLTRTLEKYPVEWTPDLVDRILKLLWNHGPKALQFFKSLDYQPTYAHVSSSFDHAIDIAGRLRDYKTLWTLVDRMRTRRLGPNPKTFAIITERYVSAGKPDRAIKIFFSMHEHGCVQDLNSFNTLLDVLCKSKRVEMADNKLFKVFRGRFRADTVSYNIMANGWCLIKRTPKALEVLKEMVKRGLNPTMTTYNILVKGFFRAGQLKEAWEFFLQMKKRKCEIDVVTYTTVVHGFGVAGEVRKAQRVFNEMIGEGVLPSVATYNAFIQVLCKKDNVENAISVFEEMLRKGYMPNSTTYNVVIRGLCHVGRMEKAMEFMARMKDDECEPNVQIYNVVIRYFCDAGEIEKGLNVFEKMGDADCLPNLDTYNILISAMFVRKKSDDLLTAGKLLIEMVERGFLPQKFTFNRVLDGLLLTGNQDFAKEILRLQSRCGRLPRRLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YW90</accession>
    <name>A0AA38YW90_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RNA-dependent RNA polymerase</fullName>
            <ecNumber evidence="9">2.7.7.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_022585</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.48"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the RdRP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.48" evidence="9"/>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YW90"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031380">
        <property type="term" value="C:nuclear RNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003968">
        <property type="term" value="F:RNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030422">
        <property type="term" value="P:siRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057590">
        <property type="entry name" value="PH_RDR1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007855">
        <property type="entry name" value="RDRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058752">
        <property type="entry name" value="RDRP_C_head"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057596">
        <property type="entry name" value="RDRP_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058751">
        <property type="entry name" value="RDRP_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058763">
        <property type="entry name" value="RRM_RDR1/2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23079">
        <property type="entry name" value="RNA-DEPENDENT RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23079:SF5">
        <property type="entry name" value="RNA-DEPENDENT RNA POLYMERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24823">
        <property type="entry name" value="PH_RDR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05183">
        <property type="entry name" value="RdRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26253">
        <property type="entry name" value="RdRP_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26252">
        <property type="entry name" value="RdRP_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26250">
        <property type="entry name" value="RRM_RdRP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 9" id="KW-0696">RNA-directed RNA polymerase</keyword>
    <keyword evidence="6 9" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RRM" evidence="10">
        <location>
            <begin position="7"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9677687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1124" mass="127079" checksum="7AD5FDC0F337D117" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YWJ5</accession>
    <name>A0AA38YWJ5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Adenine/guanine permease AZG2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_022792</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family. Azg-like subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9678015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YWJ5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005345">
        <property type="term" value="F:purine nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015853">
        <property type="term" value="P:adenine transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015854">
        <property type="term" value="P:guanine transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045018">
        <property type="entry name" value="Azg-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006043">
        <property type="entry name" value="NCS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43337:SF13">
        <property type="entry name" value="ADENINE_GUANINE PERMEASE AZG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43337">
        <property type="entry name" value="XANTHINE/URACIL PERMEASE C887.17-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00860">
        <property type="entry name" value="Xan_ur_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="106"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="154"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="190"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="271"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="331"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="408"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="482"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9678015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="528" mass="56015" checksum="33A0FD245FDC8447" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YXY4</accession>
    <name>A0AA38YXY4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Retrovirus-related Pol polyprotein from transposon RE1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_020646</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YXY4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057670">
        <property type="entry name" value="SH3_retrovirus"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439">
        <property type="entry name" value="GAG-POL-RELATED RETROTRANSPOSON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439:SF467">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25597">
        <property type="entry name" value="SH3_retrovirus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Retroviral polymerase SH3-like" evidence="2">
        <location>
            <begin position="55"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase Ty1/copia-type" evidence="1">
        <location>
            <begin position="258"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9678523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="728" mass="82479" checksum="963BF9F6D40E7E5D" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YYI1</accession>
    <name>A0AA38YYI1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_020904</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YYI1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044303">
        <property type="entry name" value="ZAT1/4/9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46326:SF10">
        <property type="entry name" value="C2H2 AND C2HC ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46326">
        <property type="entry name" value="ZINC FINGER PROTEIN ZAT1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="4"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="129"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="173"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="20"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="216"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="226"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9678852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="235" mass="26557" checksum="C0DEB8B590C4510E" modified="2024-01-24" version="1">MESAKCKICFRQFSNRKALGGHMRSHMAKLSIQPKPQKPDNSSKFAVHDDQESETDTPKNQTRRRFKRACRSINKKTDSPDSSVVSDVDDFSVEDAAQLLVLLSREKWTRGKEVDNEELMKEDNFTIIYRCETCNKGFQSYQALGGHRASHKKLKIESDEEDIAPRNGNQRTFKCPFCFKVFESGQAMGGHKKVHMSTAAVAARRVSMPGQNFIDLNLPAPEEDNGSQVDDPSIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YYQ2</accession>
    <name>A0AA38YYQ2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase M3A/M3B catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_021088</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6">Binds 1 zinc ion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase M3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YYQ2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006518">
        <property type="term" value="P:peptide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1370.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045090">
        <property type="entry name" value="Pept_M3A_M3B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001567">
        <property type="entry name" value="Pept_M3A_M3B_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11804:SF79">
        <property type="entry name" value="MITOCHONDRIAL INTERMEDIATE PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11804">
        <property type="entry name" value="PROTEASE M3 THIMET OLIGOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01432">
        <property type="entry name" value="Peptidase_M3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1 6" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M3A/M3B catalytic" evidence="7">
        <location>
            <begin position="3"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9679055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="282" mass="32108" checksum="D830675255102B79" modified="2024-01-24" version="1">MFVVALVCNFSGSRSSSSAKLNHWEVETLFHEFGHALHSLLSRTDYQHFSGTRVVLDLAETPSNLFEYYAWDYRVLRKFARHYSTGEVIPEKLVESMQGARKMFAATDLQRQIFYALIDQTLFGEQLSRQRDTTSVVADLKRQYASWKHVEGTHWQTRFNHLLNYGAGYYSYLYAKCFAATIWQKLCQEDPLSLATGTALRTKFMEHGGAKDPADLLTDLVGDGILQSRDGGIVPDLTSLCDEMKLEECGQEHVVLYPIETGGKKRITGLGRKNSHFCWEKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z0J6</accession>
    <name>A0AA38Z0J6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_021635</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z0J6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000590">
        <property type="entry name" value="PR5-like receptor kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000178">
        <property type="entry name" value="serine/threonine-protein kinase PBS1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045874">
        <property type="entry name" value="LRK10/LRL21-25-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025287">
        <property type="entry name" value="WAK_GUB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27009">
        <property type="entry name" value="RUST RESISTANCE KINASE LR10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13947">
        <property type="entry name" value="GUB_WAK_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041440203" description="Protein kinase domain-containing protein" evidence="13">
        <location>
            <begin position="22"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="289"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="317"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9679784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="601" mass="68238" checksum="01749125E267DA45" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z0U4</accession>
    <name>A0AA38Z0U4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Alpha-1,4 glucan phosphorylase</fullName>
            <ecNumber evidence="2">2.4.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_019576</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Allosteric enzyme that catalyzes the rate-limiting step in glycogen catabolism, the phosphorolytic cleavage of glycogen to produce glucose-1-phosphate, and plays a central role in maintaining cellular and organismal glucose homeostasis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>[(1-&gt;4)-alpha-D-glucosyl](n) + phosphate = [(1-&gt;4)-alpha-D-glucosyl](n-1) + alpha-D-glucose 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:41732"/>
            <dbReference type="Rhea" id="RHEA-COMP:9584"/>
            <dbReference type="Rhea" id="RHEA-COMP:9586"/>
            <dbReference type="ChEBI" id="CHEBI:15444"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="EC" id="2.4.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the glycogen phosphorylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.1" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z0U4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008184">
        <property type="term" value="F:glycogen phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005980">
        <property type="term" value="P:glycogen catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000811">
        <property type="entry name" value="Glyco_trans_35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11468:SF4">
        <property type="entry name" value="ALPHA-GLUCAN PHOSPHORYLASE 2, CYTOSOLIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11468">
        <property type="entry name" value="GLYCOGEN PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00343">
        <property type="entry name" value="Phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9680298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="119" mass="13753" checksum="70E56ED972503171" modified="2024-01-24" version="1">MSNFNDGQYEAAAHFHSRARQICAVLYPRDATESGKLLRLKQQFFLCNASLQNIIFRFKERKDGGSWQWFEFPTKVVVQLNDTHPTLAFPELMRLLMNDEGFGWDEVWDMTSKASSVGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z181</accession>
    <name>A0AA38Z181_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myb/SANT-like DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_019742</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z181"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000152">
        <property type="entry name" value="Trihelix transcription factor ASIL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044823">
        <property type="entry name" value="ASIL1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044822">
        <property type="entry name" value="Myb_DNA-bind_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31307:SF49">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE TRANSCRIPTION FACTOR MYB_SANT-LIKE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31307">
        <property type="entry name" value="TRIHELIX TRANSCRIPTION FACTOR ASIL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13837">
        <property type="entry name" value="Myb_DNA-bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00595">
        <property type="entry name" value="MADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb/SANT-like DNA-binding" evidence="2">
        <location>
            <begin position="33"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="128"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="210"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="186"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9680508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="320" mass="35348" checksum="80E7A3BC95563C33" modified="2024-01-24" version="1">MASTTSPAQDPSLPPPQQQPSPAPPSSRRLPPPCWSHDETVALIDSYRDKWYALRRGNLRANHWQEVADAVSRRCPLSSPPKTSVQCRHKMEKLRKRYRTELQRSMNAPSHRFNSSWVHFKRMDAMEKGPSSTAAVESGSDGDDDDDDDDQDLYEDGGLKRGIGNTRSLQGLMSNGIGSGGGPGAGFRIRIPGRGNLDSPPMAKIYGNFDQNPNPNPNFGSRVLGDNDRARPAIGKRSGGGGGDPVAEMVAAIKMLGDGFAKMEQKKMDMAREVEAMRMDMEMKRTEMILESQQRIVEAFANALSEKKKKPKKMTSSPEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z1I5</accession>
    <name>A0AA38Z1I5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">P53 and DNA damage-regulated protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_019851</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z1I5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030482">
        <property type="entry name" value="PDRG1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21162">
        <property type="entry name" value="P53 AND DNA DAMAGE-REGULATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21162:SF0">
        <property type="entry name" value="P53 AND DNA DAMAGE-REGULATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9680656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="166" mass="18978" checksum="6B88E19B82B27B94" modified="2024-01-24" version="1">MDESMKQLQQGLIEIEIEAENVLLARHQLVENDRVRNGNREALTALRKRARTTKTSVPSPFESIMKEIEQLESRPLVKEVCATCGNHDSRENTWMMFPGTDVFARIPFHAAHSILEKDQERLDYDAKKLQSYLKEKSFLISEKGVLADKISPGIIRSLVTLTDKPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z338</accession>
    <name>A0AA38Z338_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_020211</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant LTP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9681222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z338"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01960">
        <property type="entry name" value="nsLTP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.110.10">
        <property type="entry name" value="Plant lipid-transfer and hydrophobic proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036312">
        <property type="entry name" value="Bifun_inhib/LTP/seed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016140">
        <property type="entry name" value="Bifunc_inhib/LTP/seed_store"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000528">
        <property type="entry name" value="Plant_nsLTP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33076">
        <property type="entry name" value="NON-SPECIFIC LIPID-TRANSFER PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14368">
        <property type="entry name" value="LTP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00382">
        <property type="entry name" value="LIPIDTRNSFER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00499">
        <property type="entry name" value="AAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47699">
        <property type="entry name" value="Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041436542" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical" evidence="3">
        <location>
            <begin position="24"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9681222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="115" mass="12499" checksum="E9DEB7F3643C14EF" modified="2024-01-24" version="1">MSRVLGSLIIVLLVWSSAMAEPSCPKVISEVAPCLSFLRDDGPAKPSKRCCEGVTEVNGIAKTKQDRIAICECLKQTLNYIDYDPKRIPLLGKQCGLTIDLPPTGQNTNCSQVAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z3S9</accession>
    <name>A0AA38Z3S9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Short-chain dehydrogenase/reductase</fullName>
            <ecNumber evidence="5">1.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_020437</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.-" evidence="5"/>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9681567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z3S9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05324">
        <property type="entry name" value="carb_red_PTCR-like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000312">
        <property type="entry name" value="(+)-neomenthol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045313">
        <property type="entry name" value="CBR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43490">
        <property type="entry name" value="(+)-NEOMENTHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43490:SF126">
        <property type="entry name" value="(+)-NEOMENTHOL DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0521">NADP</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU369024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9681567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="297" mass="31995" checksum="03FA3EDC1F042EA6" modified="2024-01-24" version="1">MAEAITRSAVVTGANKGIGLEICRQLAASGVRVVLTARDEKRGVEALQSLNGSGLSNLVFHQLDVGDPASIASLADFIKTQFGKLDILVNNAGMAGSIVKDPDALRSGIAATEAGEQVNWHGIMTQPLELAEECMKINYYGPKKMVEAFIPLLQLSDSPRIVNVSSSLGKLQNVTNEWAKTVLSDAENLTEERVDEVLNQFLKDFKEGLLEAKNWSAFYAAYTVSKAALNAYTRILATKYPAFCINCVCPGFVKTDINHNIGILTVEEGAEGAVKVALLPDGGPSGHFFSRKEVTEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z4B1</accession>
    <name>A0AA38Z4B1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_018076</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z4B1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45295:SF3">
        <property type="entry name" value="CHAPERONE DNAJ-DOMAIN SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45295">
        <property type="entry name" value="CHAPERONE PROTEIN DNAJ C76, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="1">
        <location>
            <begin position="65"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9682010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="265" mass="29603" checksum="02336DAD09FECD23" modified="2024-01-24" version="1">MMGCLVLYNSTLPINTVSKWVTNLNGFNFPSTFKTSLSFSSSSTSRFTVNCRDRTREEPKLSDSSAYAVLGVDPSCSAAELKAAFRAKVKQFHPDVNKEGGDSDRMIRLVIQAYELLSCCSRSEIIERECLDPFDEPECEAFDLFVNEVLCVGKGCPYSCVNRAPHAFTFVSSTGTARATSQGHGEDYQVQLAVGQCPRSCIHYVTPSQRIVLEELLDSILDTPYDTSAEADLLYSLIIKARFENNRYQKPKKQPKGSTQHVDWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z4Z6</accession>
    <name>A0AA38Z4Z6_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_018417</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z4Z6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9682491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="160" mass="18764" checksum="95CED00BF7BDE1FF" modified="2024-01-24" version="1">MSKKIKLDFNTESGRPRDSDKFAKFSNECGYLIRKFIYKKHTLQSMCMRLWDNDIEHIDMIFTSISSNLRHNIIVQKKVVINSENCEKLTFNHRGGSKPFVFHRHHNSMSPATRELQEEVELFRATHYNEKGDTHISDLEITKRVLGKRLSYIRGLGYGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z6R4</accession>
    <name>A0AA38Z6R4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_019071</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z6R4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008266">
        <property type="term" value="F:poly(U) RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901259">
        <property type="term" value="P:chloroplast rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd21608">
        <property type="entry name" value="RRM2_NsCP33_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000268">
        <property type="entry name" value="31 kDa ribonucleoprotein, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000799">
        <property type="entry name" value="31 kDa ribonucleoprotein, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050502">
        <property type="entry name" value="Euk_RNA-bind_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048289">
        <property type="entry name" value="RRM2_NsCP33-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025:SF3">
        <property type="entry name" value="31 KDA RIBONUCLEOPROTEIN, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025">
        <property type="entry name" value="OS02G0815200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0934">Plastid</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6 8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="10">
        <location>
            <begin position="134"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="10">
        <location>
            <begin position="228"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="75"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="107"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9683329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="319" mass="35428" checksum="8FF1EA132E2DA22C" modified="2024-01-24" version="1">MASSNALLKPFSMAESCLTSVPTTLFTTKTPHPLLSLPPKPVRLFHLSCSSSSSWVSLKTKTSPTTLVPLVAQTSDWAQQEEEEEEKGGLTWDSEGGEEIEASGSEEAWEGEVGEDGEESEGSEETYSEPPEEAKLFVGNLPYDIDSEKLAQLFDEAGVVEIAEVIYNRETDQSRGFGFITMSTVEEAEKAVEMFNRYDLNGRFLTVNKAAPRGSRPERPPRAFEPSFRIYVGNLPWQVDDARLEQVFSEHGKVVDARVVYDRDTQRSRGFGFVTMSSETELNDAIAALDGQVICMPPVSYISFEISIFLSNETQYLES</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z852</accession>
    <name>A0AA38Z852_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_016587</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z852"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015020">
        <property type="term" value="F:glucuronosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044610">
        <property type="entry name" value="GLCAT14A/B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003406">
        <property type="entry name" value="Glyco_trans_14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45719:SF31">
        <property type="entry name" value="BETA-GLUCURONOSYLTRANSFERASE GLCAT14A-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45719">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02485">
        <property type="entry name" value="Branch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041225545" evidence="6">
        <location>
            <begin position="22"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9684166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="399" mass="45082" checksum="E0698FC4C76F8F9A" modified="2024-01-24" version="1">MTHLTLHLLVSLLSLSVLIFLLFFLPPPPPPTTTLTLHRHFPSNPLPPPPKLAYFISGTHGDSPRLLRLLRALYHPNNQYLLHLDRRATPQERVELSASVGSVAVFAAAENVNVVGNADAVNLDGSTPIASLLRGAAILLRYCSDWDWFVNLEASDYPLISQDDLLHILSFVPRDFNFIEHTSNIGWNEYHRIIQIVVDPGLYLASKRGIFVGTKRRVLPRQFRFFTGSPQVILSRKLVKFSILGWDNFPRTLLLFFANIKSSHRGYFQTLACNAREFSNTVMNSNLRYMAWDNPPGKEPRNPRVSDLKKMLGSGAAFAGNFAPNDHEVLDLIDSVVLHRRQGMISPGGWCVGRRDRGRDPCQHWGDINILRPGHAAERFEKLLLRVMANSTLRSNQCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z948</accession>
    <name>A0AA38Z948_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_016935</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z948"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02645">
        <property type="entry name" value="R3H_AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001088">
        <property type="entry name" value="uncharacterized protein ycf45 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058670">
        <property type="entry name" value="PTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034081">
        <property type="entry name" value="R3H_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045735">
        <property type="entry name" value="Spore_III_AA_AAA+_ATPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20953">
        <property type="entry name" value="KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20953:SF14">
        <property type="entry name" value="PROTEIN SEEDLING PLASTID DEVELOPMENT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25516">
        <property type="entry name" value="PTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19568">
        <property type="entry name" value="Spore_III_AA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="4">
        <location>
            <begin position="212"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="66"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="423"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="652"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="73"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="424"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9684708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="692" mass="75672" checksum="622A777A7704FC7B" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z9Q3</accession>
    <name>A0AA38Z9Q3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">IQ domain-containing protein IQM3-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_017021</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z9Q3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044159">
        <property type="entry name" value="IQM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31250">
        <property type="entry name" value="IQ DOMAIN-CONTAINING PROTEIN IQM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31250:SF10">
        <property type="entry name" value="IQ DOMAIN-CONTAINING PROTEIN IQM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="296"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="303"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9684830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="447" mass="50144" checksum="3DCB01A0E9D82108" modified="2024-01-24" version="1">MELQDQSHLSDYDTTAPTSHDHGSTTSSTAALKLQKVYKSYRTRRKLADSAVVVEELWWQALDFARLNHSTISFFDYVKNETAASRWSRIRLNASRVGKGLSKDARAQKLAFQHWIEAIDPRHRYGHNLNLYYEEWCKGDAGQPFFYWLDVGDGKEVELKQCPRSRLRCECIRYLGPQEREHYEYIIVEGTIVHKLSGDLLDTNGGLEGSKWIFVMSTSKRLYAGQKKKGSFHHSSFLAGGATLAAGRLMVEGGKLRSVSAYSGHYRPTDGNLSSFLVFLKEHGVNLDGVQVLSPTEDLGGDETSKTVEELSKTGLSADAELPKLQAPSDEKSKAPEPSKFAQIVRRSSYKRSLSGNLQSPRMRVPKKDILQRMKSKKEDSYQLGDQLSLKWSTGAGPRIGCVADYPLKLRVQAFEMVELTPKDPPRQLASQLVDNLTSLGSSCKLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZB16</accession>
    <name>A0AA38ZB16_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein DETOXIFICATION</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Multidrug and toxic compound extrusion protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_017661</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZB16"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015137">
        <property type="term" value="F:citrate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13136">
        <property type="entry name" value="MATE_DinF_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044644">
        <property type="entry name" value="DinF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42893:SF11">
        <property type="entry name" value="PROTEIN DETOXIFICATION 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42893">
        <property type="entry name" value="PROTEIN DETOXIFICATION 44, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="62"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="180"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="256"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="283"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="324"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="366"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="409"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="472"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="497"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="150"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9685711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="536" mass="57487" checksum="BA0CAFF57E925685" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZC08</accession>
    <name>A0AA38ZC08_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Trans-resveratrol di-O-methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_015237</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZC08"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009717">
        <property type="term" value="P:isoflavonoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000213">
        <property type="entry name" value="Coniferyl alcohol 9-O-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000057">
        <property type="entry name" value="O-methyltransferase ZRP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016461">
        <property type="entry name" value="COMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001077">
        <property type="entry name" value="COMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012967">
        <property type="entry name" value="COMT_dimerisation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11746">
        <property type="entry name" value="O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08100">
        <property type="entry name" value="Dimerisation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00891">
        <property type="entry name" value="Methyltransf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005739">
        <property type="entry name" value="O-mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51683">
        <property type="entry name" value="SAM_OMT_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="O-methyltransferase dimerisation" evidence="6">
        <location>
            <begin position="20"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="O-methyltransferase C-terminal" evidence="5">
        <location>
            <begin position="130"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR005739-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9686236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="357" mass="40310" checksum="ED94FD27746116BA" modified="2024-01-24" version="1">MDPANGEISSELLQAQAHVWNHIFNFINSMSLKCAIQLGIPDIIHNHGKPMTLPELVAKLPVHPKKTQYVYRLMRVLVQSDFFAAQRAQQSEEEEGYVLTHASRLLLKDESLSVRPFLLAMLDPTLTKPWHYVSAWFQNDDPTPFDTAHERTLWDYAGHEPQLNYFFNEAMASDARLVTSVLVKEGKGVFEGLNSLVDVGGGTGTVAKAIANAFPHLNCTVLDLPHVVAGLQGSKNLNYFAGDMFEAIPPADAILLKWILHDWSDEECVKILKRCREAIPSKEKGGKVIIIDMVMKNQKGDCKSRETQLFFDMLMMVLVTGKEREEKEWEKLFLDAGFSHYKITPILGLRSLIEVYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZCM7</accession>
    <name>A0AA38ZCM7_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_015143</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZCM7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9686112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="85" mass="9678" checksum="F48EACA4A7D9B446" modified="2024-01-24" version="1">MSCWSFGRWLQIAIGRLPERLLFVKSIICKEEILKISAAIGPLSLFETKFNIFKLRRLPISLGIPPAKLLTTRYNSSSLNDNDII</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZEK0</accession>
    <name>A0AA38ZEK0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_015687</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZEK0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF533">
        <property type="entry name" value="DYW DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9686939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="279" mass="30985" checksum="3E52BFDDC69B1FEB" modified="2024-01-24" version="1">MTQFVIGAIALKVQSRFQHALRGNDAFGLVFPTIHAYERSLPLKIATTMSRQNEELPRKFCISSMIPKQIFQMGTTGNQLHAYSIGTGLKAYTHVGNTLLSTQIRKAKLLCNGYSVKSKILMYTRRQFCCQLVPRGQFGYACRLFDQTPWMIPAVWNAIIIGCAENKHTEIALNLFLEMHQLGVRHGKYAFASVLSLCSLELLDIGREVHTLVNKTGLSVRASIVNALLTVYFNSGKVAAYEVFEEAESTVHDDITFNVTIAVLAKSHLPGQNGICPST</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZEY7</accession>
    <name>A0AA38ZEY7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_013853</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9687810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZEY7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052343">
        <property type="entry name" value="Retrotransposon-Effector_Assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46890">
        <property type="entry name" value="NON-LTR RETROLELEMENT REVERSE TRANSCRIPTASE-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46890:SF50">
        <property type="entry name" value="RNA-DIRECTED DNA POLYMERASE, EUKARYOTA, REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9687810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="90" mass="9952" checksum="E8069A4C90543BB1" modified="2024-01-24" version="1">MGFGSKWLSWMWSCISTAKFSVLVNGMPARFFPSTKGLRQGDLLSPYLFVMGMEVLSALIRRVVEGGFLSGCSIRGGKRPALNISHLFFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZG33</accession>
    <name>A0AA38ZG33_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_014088</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZG33"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000298">
        <property type="entry name" value="Adenine nucleotide alpha hydrolase-like domain kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000162">
        <property type="entry name" value="Adenine nucleotide alpha hydrolase-like domain kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000211">
        <property type="entry name" value="Dual-specific kinase DSK1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47989">
        <property type="entry name" value="OS01G0750732 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47989:SF72">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="410"/>
            <end position="686"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9688190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="753" mass="83477" checksum="ADF3B0F8FBE59551" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZGW8</accession>
    <name>A0AA38ZGW8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ethanolamine kinase</fullName>
            <ecNumber evidence="3">2.7.1.82</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_014321</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from ethanolamine: step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the choline/ethanolamine kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.82" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZGW8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004305">
        <property type="term" value="F:ethanolamine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05157">
        <property type="entry name" value="ETNK_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22603">
        <property type="entry name" value="CHOLINE/ETHANOALAMINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22603:SF66">
        <property type="entry name" value="ETHANOLAMINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01633">
        <property type="entry name" value="Choline_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00037883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9688609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="377" mass="43436" checksum="D2072E08FEB7CB1F" modified="2024-01-24" version="1">MGAGRIWDAMEVAEEARENCCSEIHSSHTTVDTSLSLPQMTPKIIELCKDLFKKWSNLDDSQFSVETISGGITNLLLKVSAKEENGNSTCMTVRLYGPNTEYVINRERELQAIGYLSAAGFGAKLLGVFGNGMVQSFINARTLTPSDMKMPKLAAEIAKQLRKFHQVEIPGSKEPQLWIDIFKFFEKASTLKFDDIEKQKKYKEISFEEVHNEVVELKELTDRLNSPVVFAHNDLLSGNLMLNDDEGKLYFIDFEYGSYNYRGFDIGNHFNEYAGYDCDYSLYPTKDEQYHFFRHYLAPDKPNEVSDQDLETLYVEANTFMLASHLYWALWASIQAKMSPIDFDYLGYYFLRYEEYTKQKEKCLSLARSYLSASRDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZH34</accession>
    <name>A0AA38ZH34_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_014543</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZH34"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9688938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="251" mass="27645" checksum="8807EA463EEDEFB2" modified="2024-01-24" version="1">MVRQPWRSLCTPGIHHPLVGMTEEYRMVPARNDDGHLILKLRMRQCNENKIGGTGFSIEGKVNQAETVTDILEVQDQPASSTKKYGPNEKNQLLFPMNPTDIVPLDHVELDFADVFGPLPVQTPSEMNPGDSGDSVSAADTNDLIYGDPVVIYSWSHSLVGPSTCLSQSSTLSKLALHEIEDSEELVECLHEEAVKELQGISSGDDISEKSFDDIDSDSTTVETVGLEDFEVMKLVGPPPASQLEKKFRYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZHI1</accession>
    <name>A0AA38ZHI1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Proteasome subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_014703</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Component of the proteasome, a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="function">
        <text evidence="2">The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of peptide bonds with very broad specificity.</text>
            <dbReference type="EC" id="3.4.25.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the 20S core complex of the 26S proteasome. The 26S proteasome is composed of a core protease (CP), known as the 20S proteasome, capped at one or both ends by the 19S regulatory particle (RP/PA700). The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the proteasome complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the peptidase T1B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9689176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZHI1"/>
    <dbReference type="SMR" id="A0AA38ZHI1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03762">
        <property type="entry name" value="proteasome_beta_type_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000035">
        <property type="entry name" value="Proteasome subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000243">
        <property type="entry name" value="Pept_T1A_subB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF0">
        <property type="entry name" value="ATP-DEPENDENT PROTEASE SUBUNIT HSLV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00141">
        <property type="entry name" value="PROTEASOME"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="8 12" id="KW-0647">Proteasome</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0888">Threonine protease</keyword>
    <keyword evidence="9" id="KW-0865">Zymogen</keyword>
    <feature type="active site" description="Nucleophile" evidence="11">
        <location>
            <position position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR600243-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9689176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="232" mass="24997" checksum="01CADCB32F4BFCD2" modified="2024-01-24" version="1">MDHLNQPHSMGTTIIGVTYNGGVVLGADSRTSTGVYVANRASDKITQLTDNVYLCRSGSAADSQILSDYVRYYLHQHTIQLGQPATVKVAANLVRLISYNNKNMLQTGLIVGGWDKHEGGKIYGVPLGGTILEQPFAIGGSGSSYLYGFFDQEWKEGMTKDEAEKLVVKAVSLAIARDGASGGVVRTVTINSEGVTKNFYPGDTLPLWHEELEPQNSLLDILSSSSPEPMVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZIH5</accession>
    <name>A0AA38ZIH5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">40S ribosomal protein S12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_014568</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZIH5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000013">
        <property type="entry name" value="40S ribosomal protein S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000530">
        <property type="entry name" value="Ribosomal_eS12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047860">
        <property type="entry name" value="Ribosomal_eS12_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11843">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00972">
        <property type="entry name" value="RIBSOMALS12E"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01189">
        <property type="entry name" value="RIBOSOMAL_S12E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="5">
        <location>
            <begin position="33"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9688978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="147" mass="15806" checksum="80D83DB1439B1E75" modified="2024-01-24" version="1">MSLWWHNELSVDEAVVVEAVSAPLGEAMDLMTALQLVLRKSLAHGGLVRGLHEGAKVIEKHAAQLCVLAEDCNQPDYIKLVKALCADHNVSLISVPSAKSLGEWAGLCKIDSEGKARKVVGCSCVVVKDYGEETPGLHIVQEHVKSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZIQ7</accession>
    <name>A0AA38ZIQ7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_014851</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the RING-type zinc finger family. ATL subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9689367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZIQ7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16454">
        <property type="entry name" value="RING-H2_PA-TM-RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053238">
        <property type="entry name" value="RING-H2_zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14155:SF610">
        <property type="entry name" value="OS01G0755700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14155">
        <property type="entry name" value="RING FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="84"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9689367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="131" mass="14858" checksum="037C806858E3A708" modified="2024-01-24" version="1">MTLFLSTWPIFLSLNDGDRQNPETDIEIGDMNLNHDDHHPHSHDSTATTYDISQRSIEQKLMEMAPIFVYGGEGGVVKCSTSECVICWEDFEDGEICRVLPACNHVFHKACVGLWLMEKRVCPLCRVCVDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZJ47</accession>
    <name>A0AA38ZJ47_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Terpene cyclase/mutase family member</fullName>
            <ecNumber evidence="4">5.4.99.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_014723</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the terpene cyclase/mutase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.-" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9689204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZJ47"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016871">
        <property type="term" value="F:cycloartenol synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016104">
        <property type="term" value="P:triterpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02892">
        <property type="entry name" value="SQCY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.20:FF:000002">
        <property type="entry name" value="Terpene cyclase/mutase family member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.20:FF:000022">
        <property type="entry name" value="Terpene cyclase/mutase family member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032696">
        <property type="entry name" value="SQ_cyclase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032697">
        <property type="entry name" value="SQ_cyclase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018333">
        <property type="entry name" value="Squalene_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002365">
        <property type="entry name" value="Terpene_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01787">
        <property type="entry name" value="squalene_cyclas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11764:SF20">
        <property type="entry name" value="LANOSTEROL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11764">
        <property type="entry name" value="TERPENE CYCLASE/MUTASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13243">
        <property type="entry name" value="SQHop_cyclase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13249">
        <property type="entry name" value="SQHop_cyclase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01016">
        <property type="entry name" value="Prenyltransferase_Like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01074">
        <property type="entry name" value="TERPENE_SYNTHASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Squalene cyclase N-terminal" evidence="6">
        <location>
            <begin position="98"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="Squalene cyclase C-terminal" evidence="5">
        <location>
            <begin position="413"/>
            <end position="750"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9689204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="760" mass="86464" checksum="E4985A9CB147455D" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZJM4</accession>
    <name>A0AA38ZJM4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">4-coumarate--CoA ligase</fullName>
            <ecNumber evidence="2">6.2.1.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_014923</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(E)-4-coumarate + ATP + CoA = (E)-4-coumaroyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19641"/>
            <dbReference type="ChEBI" id="CHEBI:12876"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:85008"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.12"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:19642"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.12" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9689474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZJM4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016207">
        <property type="term" value="F:4-coumarate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05904">
        <property type="entry name" value="4CL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000007">
        <property type="entry name" value="4-coumarate--CoA ligase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000003">
        <property type="entry name" value="Long-chain-fatty-acid--CoA ligase FadD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096:SF149">
        <property type="entry name" value="AMP-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="7">
        <location>
            <begin position="31"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="8">
        <location>
            <begin position="448"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9689474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="539" mass="58849" checksum="321D7BB2E424E8A5" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZLQ5</accession>
    <name>A0AA38ZLQ5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_013559</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZLQ5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF78">
        <property type="entry name" value="OS12G0587133 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="207"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9691416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="706" mass="80784" checksum="BDAD7FAB58408893" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZMB0</accession>
    <name>A0AA38ZMB0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Prolyl endopeptidase</fullName>
            <ecNumber evidence="7">3.4.21.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_013803</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Serine peptidase whose precise substrate specificity remains unclear. Does not cleave peptides after a arginine or lysine residue. Regulates trans-Golgi network morphology and sorting by regulating the membrane binding of the AP-1 complex. May play a role in the regulation of synaptic vesicle exocytosis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of Pro-|-Xaa &gt;&gt; Ala-|-Xaa in oligopeptides.</text>
            <dbReference type="EC" id="3.4.21.26"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the peptidase S9A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.-" evidence="7"/>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZMB0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000005">
        <property type="entry name" value="Prolyl endopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.120">
        <property type="entry name" value="Prolyl oligopeptidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023302">
        <property type="entry name" value="Pept_S9A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002470">
        <property type="entry name" value="Peptidase_S9A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051543">
        <property type="entry name" value="Serine_Peptidase_S9A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11757:SF19">
        <property type="entry name" value="PROLYL ENDOPEPTIDASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11757">
        <property type="entry name" value="PROTEASE FAMILY S9A OLIGOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02897">
        <property type="entry name" value="Peptidase_S9_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00862">
        <property type="entry name" value="PROLIGOPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50993">
        <property type="entry name" value="Peptidase/esterase 'gauge' domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Peptidase S9A N-terminal" evidence="9">
        <location>
            <begin position="17"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S9A N-terminal" evidence="9">
        <location>
            <begin position="255"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="8">
        <location>
            <begin position="477"/>
            <end position="693"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00045448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU368024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9691726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="703" mass="79659" checksum="8E312801C5000BD6" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZNW2</accession>
    <name>A0AA38ZNW2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_011562</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZNW2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000001">
        <property type="entry name" value="zinc finger CCCH domain-containing protein 15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045877">
        <property type="entry name" value="ZFP36-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547">
        <property type="entry name" value="CCCH ZINC FINGER/TIS11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547:SF121">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="71"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="128"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="211"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="71"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="128"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="211"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="246"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9692571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="316" mass="35348" checksum="3EC435500201C4C7" modified="2024-01-24" version="1">MSFPTNTPPVFAPYAEFSGDALECWPQFPMDRSSENCPHIETPSFKKPKISETVTNLRMPGSNNKVMTDTFFKTQLCIKFRLGTCINGDECNFAHGTGDIRRPLPHGQELSCKEGYVAGIWNRDHRLNSKMKLCRIFSRGEKCPYGERCNFLHEGLEKCREGSGKFRESSSISIGCTGSARGYRNGSGQLEFKRFGDSSLNANQVNANPGFWRTRLCHKWEMSGNCAYREKCVFAHGQAELRKHAVQPEGGKEHAAPKTLPSAKAGALTHKTGPGATYNQPLQEKKCAFKWEKLGKINGIYADWIDDMPLVHSSVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZPE3</accession>
    <name>A0AA38ZPE3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">GPI mannosyltransferase 1</fullName>
            <ecNumber evidence="11">2.4.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">GPI mannosyltransferase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_011524</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalytic subunit of the glycosylphosphatidylinositol-mannosyltransferase I complex which catalyzes the transfer of the first mannose, via an alpha-1,4 bond from a dolichol-phosphate-mannose (Dol-P-Man) to the glucosaminyl acyl phosphatidylinositol (GlcN-(acyl)PI) intermediate to generate alpha-D-Man-(1-&gt;4)-alpha-D-GlcN-(1-&gt;6)-(1-radyl,2-acyl-sn-glycero-3-phospho)-2-acyl-inositol and participates in the sixth step of the glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Endoplasmic reticulum membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the PIGM family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="11"/>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZPE3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990529">
        <property type="term" value="C:glycosylphosphatidylinositol-mannosyltransferase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051751">
        <property type="term" value="F:alpha-1,4-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004376">
        <property type="term" value="F:GPI mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007704">
        <property type="entry name" value="PIG-M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12886:SF0">
        <property type="entry name" value="GPI MANNOSYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12886">
        <property type="entry name" value="PIG-M MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05007">
        <property type="entry name" value="Mannosyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4 11" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="6 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041409936" description="GPI mannosyltransferase 1" evidence="12">
        <location>
            <begin position="22"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="132"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="156"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="229"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="300"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="334"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="357"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="391"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU365064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9692510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="438" mass="50809" checksum="9FCEEBD27089E262" modified="2024-01-24" version="1">MDVRSLLLFSALLRVLLIIYGEWQDAHMEVRYTDVDYLVFSDAASLMASGKSPYQRSTYRYSPLLAFLLVPNSIIHPSWGKFLFSASDLLVGLFIHTILELRKVPENLCTCALMVWLFNPFTFSIGTRGNCEPIVCAMILWIIICLMNGKVLQAAFWYGLVVHFRIYPIIYSIPIILVLDPHCFQYGLKPALSSWVGQGNSTQSIPSTAVPDSCGIWIAWTSMFTRARIMFGLISGAVFFAFTGLFFYLYRWEFLHEALLYHLTRTDPRHNFSIYFYHIYLHYEHEFSVVERLISFLPQFLIQLVLIICFAQDLPFCFFAQTVAFVAFNKVITAQYFVWFFCLLPLILPWSNMKLKWTGLSCILLWMGAQTHWLMWGYLLEFKGKNVFLELWIASILFLAANTFVLIKIIRYHTFSPLFQRLEFSSSDNDLGEGKKSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZQ31</accession>
    <name>A0AA38ZQ31_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_011402</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZQ31"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36362">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36362:SF1">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9692323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="428" mass="49198" checksum="E67B41474FB6169A" modified="2024-01-24" version="1">MLERMLSARRAPLHSDEAEVEDDESKTRKHISFAMRASTYLTRLGYLLPCLLLGVVLSCIILISTWSRRLACAPSSDAASRFSFFGFDGPDSDFGSLGVPWCRSKHGKTVQWTSKDLLKGLEEFVPIYEKRPIKNNMYGMGFDHSFGLWFMAQWLKPDLMIESGAFKGHSTWVLRQAMPDTRIVSLSPRHPEKYLKKGPAYVDGNCTYFAGKDFVDFGSVDWRSVMKQHGITDLSRVLIFFDDHQNELKRIKQALKAGFQHLIFEDNYDTGTGDHYSLRQICDQFYIRGGGHSCFRDSDEARERAKRKKFWEKAVDTDELCGAGEEWWGVRGQMRDNFNHSNKDISYTEHFMNSRFVESVLDVYWELPPVAGPSLTHQSRYDPARVSSPIVEDGRFGLFQRLGLGRLEPFVFNGYTQMVYLQISEQES</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZQ65</accession>
    <name>A0AA38ZQ65_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_011428</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZQ65"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.430:FF:000003">
        <property type="entry name" value="Probable disease resistance protein At5g66910"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057135">
        <property type="entry name" value="At4g27190-like_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050905">
        <property type="entry name" value="Plant_NBS-LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463:SF220">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23247">
        <property type="entry name" value="LRR_RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NB-ARC" evidence="8">
        <location>
            <begin position="156"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="10">
        <location>
            <begin position="411"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="11">
        <location>
            <begin position="543"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein At4g27190-like leucine-rich repeats" evidence="9">
        <location>
            <begin position="770"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="32"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9692369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="911" mass="104774" checksum="3F5D97DCF9C91B02" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZQI2</accession>
    <name>A0AA38ZQI2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Hydroxyisourate hydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_012258</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate + H(+) = (S)-allantoin + CO2</text>
            <dbReference type="Rhea" id="RHEA:26301"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15678"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58639"/>
            <dbReference type="EC" id="4.1.1.97"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-hydroxyisourate + H2O = 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23736"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18072"/>
            <dbReference type="ChEBI" id="CHEBI:58639"/>
            <dbReference type="EC" id="3.5.2.17"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Purine metabolism; urate degradation; (S)-allantoin from urate: step 3/3.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9693408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZQI2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051997">
        <property type="term" value="F:2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033971">
        <property type="term" value="F:hydroxyisourate hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006144">
        <property type="term" value="P:purine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019628">
        <property type="term" value="P:urate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05822">
        <property type="entry name" value="TLP_HIUase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3330.10:FF:000002">
        <property type="entry name" value="Uric acid degradation bifunctional protein TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.180:FF:000003">
        <property type="entry name" value="Uric acid degradation bifunctional protein TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3330.10">
        <property type="entry name" value="Oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.180">
        <property type="entry name" value="Transthyretin/hydroxyisourate hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014306">
        <property type="entry name" value="Hydroxyisourate_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018020">
        <property type="entry name" value="OHCU_decarboxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036778">
        <property type="entry name" value="OHCU_decarboxylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023418">
        <property type="entry name" value="Thyroxine_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000895">
        <property type="entry name" value="Transthyretin/HIU_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023416">
        <property type="entry name" value="Transthyretin/HIU_hydrolase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036817">
        <property type="entry name" value="Transthyretin/HIU_hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023419">
        <property type="entry name" value="Transthyretin_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02962">
        <property type="entry name" value="hdxy_isourate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43466">
        <property type="entry name" value="2-OXO-4-HYDROXY-4-CARBOXY-5-UREIDOIMIDAZOLINE DECARBOXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43466:SF1">
        <property type="entry name" value="2-OXO-4-HYDROXY-4-CARBOXY-5-UREIDOIMIDAZOLINE DECARBOXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09349">
        <property type="entry name" value="OHCU_decarbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00576">
        <property type="entry name" value="Transthyretin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00189">
        <property type="entry name" value="TRNSTHYRETIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49472">
        <property type="entry name" value="Transthyretin (synonym: prealbumin)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158694">
        <property type="entry name" value="UraD-Like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00768">
        <property type="entry name" value="TRANSTHYRETIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00769">
        <property type="entry name" value="TRANSTHYRETIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0659">Purine metabolism</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase" evidence="11">
        <location>
            <begin position="7"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="Transthyretin/hydroxyisourate hydrolase" evidence="10">
        <location>
            <begin position="215"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="63"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="331"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR600895-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF09349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9693408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="334" mass="36946" checksum="14442D680EDEFAAB" modified="2024-01-24" version="1">MDSKMEEKDFLACCGSKKFAEEMTSSGPFANLDHAIDVARDIWFNKVDVNGWLEAFAAHPQIGQNPSAKHPSDTSAQWSKGEQSTALQTATDSSLQELSDWNARYWKKFGFVFLICASGRTASEILAELKRRYPNRPIVEFEIAAQEQMKVTELRLAKLFSTQVKAASISTQNPETAAKKAGEDRVSIIGAHLTATSEASAGKTPQISPRTRPPITSHVLDVARGSPAAGIEVRLEMWKGNQPRPLFGKEDEGGWVLEGSSITDKDGRSGQLMSMIDALNPGIYKISFNTGKYCPSGFFPYVSILFEIRESQKWEHFHVPLLLSPFSFSTYRGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZQZ3</accession>
    <name>A0AA38ZQZ3_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_012063</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9693162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZQZ3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050317">
        <property type="entry name" value="Plant_Fungal_Acyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31642:SF266">
        <property type="entry name" value="HXXXD-TYPE ACYL-TRANSFERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31642">
        <property type="entry name" value="TRICHOTHECENE 3-O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9693162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="471" mass="51465" checksum="C7F9B02F03E4758E" modified="2024-01-24" version="1">MGGGGDFMVTVSRKEVVAAALPVQEYWLPQSNLDLLLPPVDVGVFFCYKKPHGSGNGGDELRFGSMVRVVKEALAQALVSYYAFAGEVVSNSVGEPELLCNNRGVDFTEAYADVQLQDLNLYNPDDSIESKLVPKKKNGVLSVQVTELKCGGAVVGCTFDHRIADAYSANLFLVSWAEMAQSKPLSVIPSFRRSLLNPRRPGSYHPSLDEMYVPISALPPPKAPQPGADHLISRLYYVSAEQLSLLQTLASSGGIRKRTKLESLSAFLWKMVAKSAVMENANKKICKMGIVVDGRGRVSSGDEDKTALMASYFGNVLSIPFGEKIIGDLKEQPLSWVADAVHDYLERAVTKEHFLGLIDWVEAHRPEPALAKIYCSGSSDGPAFVVSSGQRFPASKVDFGWGRPALGSYHFPWGGDAGYVMPMPSPARDGDWVVYMHLQNGQIELIESQGAHIFRPLTSDYLKLADFPRLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZQZ8</accession>
    <name>A0AA38ZQZ8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">K Homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_009498</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9693572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZQZ8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048024">
        <property type="term" value="P:regulation of mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045071">
        <property type="entry name" value="BBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055256">
        <property type="entry name" value="KH_1_KHDC4/BBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032377">
        <property type="entry name" value="STAR_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11208:SF42">
        <property type="entry name" value="QUAKING RELATED 54B, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11208">
        <property type="entry name" value="RNA-BINDING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22675">
        <property type="entry name" value="KH-I_KHDC4-BBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16544">
        <property type="entry name" value="STAR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="3">
        <location>
            <begin position="131"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="253"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9693572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="281" mass="31232" checksum="EE177C4956A0D168" modified="2024-01-24" version="1">MSGLYNHNFSPARAVSPQIRSTPDVDSQYLSELLAEHQKLGPFMQVLPICSRLLNQEIIRVSGMISNQGFGDFDRLQHRSPSPMASSNLMSNVAGTGLGGWNGLPQERLSGPHGMTMDWQGAPASPSSYTVKRILRLEIPVDTYPNFNFVGRLLGPRGNSLKRVEATTGCRVYIRGKGSIKDPEKEDKLRGRPGYEHLNDPLHILIEADLPANIVDMRLRQAQEIIEELLKPVDESQDFIKRQQLRELALLNSNFREESPGPSGSVSPFNTSGMKRAKTGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZRU0</accession>
    <name>A0AA38ZRU0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">DNA N(6)-methyladenine demethylase</fullName>
            <ecNumber evidence="13">1.14.11.51</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_009884</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>an N(6)-methyl-2'-deoxyadenosine in DNA + 2-oxoglutarate + O2 = a 2'-deoxyadenosine in DNA + formaldehyde + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:49524"/>
            <dbReference type="Rhea" id="RHEA-COMP:12418"/>
            <dbReference type="Rhea" id="RHEA-COMP:12419"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:90615"/>
            <dbReference type="ChEBI" id="CHEBI:90616"/>
            <dbReference type="EC" id="1.14.11.51"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:49525"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="14">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the alkB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.51" evidence="13"/>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZRU0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035516">
        <property type="term" value="F:broad specificity oxidative DNA demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141131">
        <property type="term" value="F:DNA N6-methyladenine demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035515">
        <property type="term" value="F:oxidative RNA demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035513">
        <property type="term" value="P:oxidative RNA demethylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.590:FF:000013">
        <property type="entry name" value="2-oxoglutarate-dependent dioxygenase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.590">
        <property type="entry name" value="Alpha-ketoglutarate-dependent dioxygenase AlkB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004574">
        <property type="entry name" value="Alkb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027450">
        <property type="entry name" value="AlkB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037151">
        <property type="entry name" value="AlkB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16557:SF10">
        <property type="entry name" value="2-OXOGLUTARATE-DEPENDENT DIOXYGENASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16557">
        <property type="entry name" value="ALKYLATED DNA REPAIR PROTEIN ALKB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13532">
        <property type="entry name" value="2OG-FeII_Oxy_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9 14" id="KW-0408">Iron</keyword>
    <keyword evidence="5 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="16">
        <location>
            <begin position="406"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="100"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="126"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="192"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="215"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="424"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="426"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="484"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00052047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00066586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR604574-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9694116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="516" mass="57779" checksum="69864CDB8871F1F5" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZS24</accession>
    <name>A0AA38ZS24_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_009605</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9693732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZS24"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000535">
        <property type="entry name" value="MSP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008962">
        <property type="entry name" value="PapD-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF165">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00635">
        <property type="entry name" value="Motile_Sperm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49354">
        <property type="entry name" value="PapD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50202">
        <property type="entry name" value="MSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="MSP" evidence="4">
        <location>
            <begin position="4"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="172"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="205"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="238"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="271"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="304"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="391"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="424"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9693732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="539" mass="58377" checksum="C417142A116A64C6" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZUT3</accession>
    <name>A0AA38ZUT3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">3,9-dihydroxypterocarpan 6A-monooxygenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_010937</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZUT3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20655">
        <property type="entry name" value="CYP93"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000019">
        <property type="entry name" value="Cytochrome P450 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47944:SF17">
        <property type="entry name" value="3,9-DIHYDROXYPTEROCARPAN 6A-MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47944">
        <property type="entry name" value="CYTOCHROME P450 98A9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12 13" id="KW-0349">Heme</keyword>
    <keyword evidence="9 12 13" id="KW-0408">Iron</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 13" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="8 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="12">
        <location>
            <position position="455"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9695706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="518" mass="58491" checksum="11176965A404CC39" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZUU5</accession>
    <name>A0AA38ZUU5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">PVL29_010300</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="23">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the leguminous lectin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZUU5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06899">
        <property type="entry name" value="lectin_legume_LecRK_Arcelin_ConA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000108">
        <property type="entry name" value="L-type lectin-domain containing receptor kinase S.4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000051">
        <property type="entry name" value="L-type lectin-domain containing receptor kinase V.9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000112">
        <property type="entry name" value="Lectin-domain containing receptor kinase A4.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050528">
        <property type="entry name" value="L-type_Lectin-RKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001220">
        <property type="entry name" value="Legume_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27007">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00139">
        <property type="entry name" value="Lectin_legB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0430">Lectin</keyword>
    <keyword evidence="14 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8 21" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041463721" description="non-specific serine/threonine protein kinase" evidence="21">
        <location>
            <begin position="31"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="296"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="355"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="384"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAJ9694753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="679" mass="75348" checksum="5E7C10D1EC882BBB" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A0T9</accession>
    <name>A0AA39A0T9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Inositol 3-kinase</fullName>
            <ecNumber evidence="7">2.7.1.64</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Myo-inositol kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_007641</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>myo-inositol + ATP = 1D-myo-inositol 3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21804"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58401"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.64"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the carbohydrate kinase PfkB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.64" evidence="7"/>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A0T9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019140">
        <property type="term" value="F:inositol 3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010264">
        <property type="term" value="P:myo-inositol hexakisphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1190.20:FF:000026">
        <property type="entry name" value="Inositol 3-kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002173">
        <property type="entry name" value="Carboh/pur_kinase_PfkB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050306">
        <property type="entry name" value="PfkB_Carbo_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43085">
        <property type="entry name" value="HEXOKINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43085:SF13">
        <property type="entry name" value="INOSITOL 3-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00584">
        <property type="entry name" value="PFKB_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase PfkB" evidence="10">
        <location>
            <begin position="206"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00050311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00066381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00071684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00077001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9698674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="381" mass="41399" checksum="CF01AB8DB463BB9D" modified="2024-01-24" version="1">MLVEQKQPNMGRRGLVVGNYCHDVLIRNDVVLGETLGGAASFVSNVLDGVSIRCDLVSKVGSDFAYSVAHPPIAVPTSPTTLFHAFFDSGSDGEGACDRVLKRVRFCGAIEPSDLPESRFNFGMAVGVAGEILPETLERMLDICEVVFVDIQALIRVFDSSDGTVRLVGLKESGFFHLLPRVGFLKASAEEAPYVDVEEARKWCCVLITNGKEGCTLYWKDGEMQISPFPTVQIDPTGAGDSFLGGFVAGLVQGLAVPDAALLGNLFGSMTVGQIGLPKFELRLLQRVKDEVQRRKMQSMSCCEDSNDDLKFVKPAGHQQFHSSLAAAKLASTAPLQECQWDLRNSPPRAVEQGIPQYTGQQKLLLNPVYEEPIQSVEGTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A1K0</accession>
    <name>A0AA39A1K0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pectinesterase</fullName>
            <ecNumber evidence="7">3.1.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_007927</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>[(1-&gt;4)-alpha-D-galacturonosyl methyl ester](n) + n H2O = [(1-&gt;4)-alpha-D-galacturonosyl](n) + n methanol + n H(+)</text>
            <dbReference type="Rhea" id="RHEA:22380"/>
            <dbReference type="Rhea" id="RHEA-COMP:14570"/>
            <dbReference type="Rhea" id="RHEA-COMP:14573"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17790"/>
            <dbReference type="ChEBI" id="CHEBI:140522"/>
            <dbReference type="ChEBI" id="CHEBI:140523"/>
            <dbReference type="EC" id="3.1.1.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the pectinesterase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the PMEI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.11" evidence="7"/>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9699079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A1K0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004857">
        <property type="term" value="F:enzyme inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030599">
        <property type="term" value="F:pectinesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042545">
        <property type="term" value="P:cell wall modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045490">
        <property type="term" value="P:pectin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd15798">
        <property type="entry name" value="PMEI-like_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.40:FF:000013">
        <property type="entry name" value="Pectinesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000001">
        <property type="entry name" value="Pectinesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.40">
        <property type="entry name" value="Invertase/pectin methylesterase inhibitor family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035513">
        <property type="entry name" value="Invertase/methylesterase_inhib"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033131">
        <property type="entry name" value="Pectinesterase_Asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000070">
        <property type="entry name" value="Pectinesterase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006501">
        <property type="entry name" value="Pectinesterase_inhib_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01614">
        <property type="entry name" value="PME_inhib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31707">
        <property type="entry name" value="PECTINESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01095">
        <property type="entry name" value="Pectinesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04043">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00856">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101148">
        <property type="entry name" value="Plant invertase/pectin methylesterase inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00503">
        <property type="entry name" value="PECTINESTERASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0063">Aspartyl esterase</keyword>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041486289" description="Pectinesterase" evidence="7">
        <location>
            <begin position="21"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="domain" description="Pectinesterase inhibitor" evidence="8">
        <location>
            <begin position="19"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9699079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="512" mass="55752" checksum="2C7E226FAD06DC3E" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A385</accession>
    <name>A0AA39A385_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">COBRA C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_005535</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the COBRA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9699710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A385"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010215">
        <property type="term" value="P:cellulose microfibril organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056900">
        <property type="entry name" value="COB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006918">
        <property type="entry name" value="COBRA_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31052">
        <property type="entry name" value="COBRA-LIKE PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31052:SF3">
        <property type="entry name" value="COBRA-LIKE PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25079">
        <property type="entry name" value="COB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04833">
        <property type="entry name" value="COBRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041327729" description="COBRA C-terminal domain-containing protein" evidence="9">
        <location>
            <begin position="25"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="domain" description="COBRA C-terminal" evidence="10">
        <location>
            <begin position="413"/>
            <end position="624"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF25079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9699710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="651" mass="70910" checksum="4E1521FD2FCB0AB1" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A3P6</accession>
    <name>A0AA39A3P6_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_005952</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A3P6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.720">
        <property type="entry name" value="Pollen allergen ole e 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015333">
        <property type="entry name" value="Pollen_allergen_ole-e-6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036466">
        <property type="entry name" value="Pollen_allergen_ole-e-6_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09253">
        <property type="entry name" value="Ole_e_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111388">
        <property type="entry name" value="Pollen allergen ole e 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041417701" evidence="1">
        <location>
            <begin position="27"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9700395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="86" mass="9349" checksum="DAF7C6664E7B1CCC" modified="2024-01-24" version="1">MANNVAALLVVCMVVAAVDHLRKTEAEVDMFASCFDSCHKRCESEENGDARCEMKCDADCVSVETAGMIPFIVLLINHASLLLCLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A5A2</accession>
    <name>A0AA39A5A2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Probable magnesium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_006449</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 8">Acts as a Mg(2+) transporter. Can also transport other divalent cations such as Fe(2+), Sr(2+), Ba(2+), Mn(2+) and Co(2+) but to a much less extent than Mg(2+).</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Early endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the NIPA (TC 2.A.7) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A5A2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008521">
        <property type="entry name" value="Mg_trans_NIPA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12570:SF20">
        <property type="entry name" value="MAGNESIUM TRANSPORTER NIPA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05653">
        <property type="entry name" value="Mg_trans_NIPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 8" id="KW-0967">Endosome</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="49"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="147"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="174"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="210"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="243"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="275"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9701101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="316" mass="34572" checksum="AAC56E99C2C91FF0" modified="2024-01-24" version="1">MGNSSDNVRGLILALSSSIFIGSSFIVKKKGLRKAGATGVRAGVGGYSYLYEPLWWAGMITMIFGEIANFAAYAFAPAILVTPLGALSIIFSAVLAHFILEEKLHIFGILGCVLCVVGSTSIVLHAPQEKDIESVKEVWNLATEPGFIVYTSIVLVLVLILIFRFVPRYGSTHMIVYVGICSLMGSLTVMSVKALAIALKLTFLGTNQFIYFQTWFFTMVVIVCCLLQVNYLNKALDTFNTAVISPVYYVMFTTLTILASMIMFKDWDTQNGSQIVTELCGFVTILSGTFLLHKTKDMGNEESKHSSISLASQAKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ABX8</accession>
    <name>A0AA39ABX8_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_002780</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ABX8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33935">
        <property type="entry name" value="OS10G0148100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33935:SF22">
        <property type="entry name" value="OS10G0149400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01190">
        <property type="entry name" value="Pollen_Ole_e_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9704374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="536" mass="59990" checksum="B31A502A053196CC" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ACE7</accession>
    <name>A0AA39ACE7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_002847</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ACE7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12283">
        <property type="entry name" value="RRM1_RBM39_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12284">
        <property type="entry name" value="RRM2_RBM23_RBM39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12285">
        <property type="entry name" value="RRM3_RBM39_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000601">
        <property type="entry name" value="CC1-like, splicing factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000326">
        <property type="entry name" value="RNA-binding protein 39 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000276">
        <property type="entry name" value="Splicing factor, CC1-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029123">
        <property type="entry name" value="RBM39_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006509">
        <property type="entry name" value="RBM39_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01622">
        <property type="entry name" value="SF-CC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48036">
        <property type="entry name" value="SPLICING FACTOR (PAD-1), PUTATIVE (AFU_ORTHOLOGUE AFUA_1G15810)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15519">
        <property type="entry name" value="RBM39linker"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="219"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="323"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="520"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="8"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="30"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="62"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9704472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="609" mass="69122" checksum="EE10D60720C249EE" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ACT5</accession>
    <name>A0AA39ACT5_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_003282</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ACT5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007541">
        <property type="entry name" value="Uncharacterised_BSP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33321">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33321:SF12">
        <property type="entry name" value="PLANT BASIC SECRETORY PROTEIN (BSP) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04450">
        <property type="entry name" value="BSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041414417" evidence="1">
        <location>
            <begin position="24"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9705151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="226" mass="25350" checksum="80E5A18BB73FFFAF" modified="2024-01-24" version="1">MGSRHMVLLSCFVFLAALHGIQAVEYAVTNNAGSSAGGVRFTNEIGIPYSRQTLVSATDFIWGVFQQNTPAERKTVQKITLIIENMNGVAYSSNNEIHVNANYIGNYQGDVKREFTGVLYHEMTHIWQWNGNGQTPGGLIEGIADFVRLKANYAPSHWVQPGQGDRWDQGYDVTARFLDYCNSLRNGFVAELNKKMRSGYSADFFVQLLGKTVDQLWTDYKAKYGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AD56</accession>
    <name>A0AA39AD56_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_003085</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AD56"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025322">
        <property type="entry name" value="PADRE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33052">
        <property type="entry name" value="DUF4228 DOMAIN PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14009">
        <property type="entry name" value="PADRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="143"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="164"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9704870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="174" mass="18867" checksum="5060971FFD4CEB97" modified="2024-01-24" version="1">MGICSSCESTSVATAKLILQDGKLQEVPCPVKVSFLLQKNPACFICNSDDMEFDDFISAINSDDELQPDQLYFALPMSWLKRPLRAEDMASLAVKASAALMRGGAKGCGCRGKGVLGQVAFSQKEYVKATRLVDADDGSGRLIEKRREKGGGGRGQHGRRGRDFTTNLSMVPEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AEQ3</accession>
    <name>A0AA39AEQ3_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_001632</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AEQ3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051341">
        <property type="entry name" value="Zyg-11_UBL_adapter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12904">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12904:SF23">
        <property type="entry name" value="PROTEIN ZER-1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="526"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9706172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="589" mass="65324" checksum="CC7B39F0A510C5C2" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AF62</accession>
    <name>A0AA39AF62_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase MurE homolog, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_001799</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Component of the plastid-encoded plastid RNA polymerase (PEP) complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MurCDEF family. MurE subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AF62"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016881">
        <property type="term" value="F:acid-amino acid ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.20:FF:000006">
        <property type="entry name" value="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="entry name" value="Mur ligase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="entry name" value="Mur-like, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1390.10">
        <property type="entry name" value="MurE/MurF, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00208">
        <property type="entry name" value="MurE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004101">
        <property type="entry name" value="Mur_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000713">
        <property type="entry name" value="Mur_ligase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035911">
        <property type="entry name" value="MurE/MurF_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005761">
        <property type="entry name" value="UDP-N-AcMur-Glu-dNH2Pim_ligase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01085">
        <property type="entry name" value="murE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001126">
        <property type="entry name" value="PRK00139.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23135">
        <property type="entry name" value="MUR LIGASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23135:SF4">
        <property type="entry name" value="UDP-N-ACETYLMURAMOYL-L-ALANYL-D-GLUTAMATE--2,6-DIAMINOPIMELATE LIGASE MURE HOMOLOG, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01225">
        <property type="entry name" value="Mur_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02875">
        <property type="entry name" value="Mur_ligase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="MurD-like peptide ligases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="MurD-like peptide ligases, peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63418">
        <property type="entry name" value="MurE/MurF N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mur ligase N-terminal catalytic" evidence="5">
        <location>
            <begin position="264"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="Mur ligase central" evidence="7">
        <location>
            <begin position="354"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="domain" description="Mur ligase C-terminal" evidence="6">
        <location>
            <begin position="581"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="187"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="190"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="207"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00064883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00072427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9706476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="774" mass="85421" checksum="9768C95D5DF5A2F6" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AFF6</accession>
    <name>A0AA39AFF6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Neprosin PEP catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_001839</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AFF6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053168">
        <property type="entry name" value="Glutamic_endopeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004314">
        <property type="entry name" value="Neprosin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31589:SF223">
        <property type="entry name" value="PROTEIN, PUTATIVE (DUF239)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31589">
        <property type="entry name" value="PROTEIN, PUTATIVE (DUF239)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03080">
        <property type="entry name" value="Neprosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52045">
        <property type="entry name" value="NEPROSIN_PEP_CD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="Neprosin PEP catalytic" evidence="2">
        <location>
            <begin position="190"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS52045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9706562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="433" mass="47434" checksum="25605898713CB06F" modified="2024-01-24" version="1">MLGGKSDRVDVTICIGTNIDGFGGSRPDGLDICTKDTIDVPNSILNGVTKEASTCSERGSTYGMRVLKVKSMGLRVVLPYLLVCYLFLYYNGVEGYRKLSKEEDLEIEKELKRLNKPAVKTIKMRPTSPPRRVSPEKEVPKPDYKQVKLGLEGGGCPMGTVPIRRTTKDDLIRAKLYSEMHASKINPLTDEEPGKHFAVARTTENIDYDGVGAILSVWNLPAQAPQYTSGRVKIKNGAESLEAGWTVNPGLYGGDNRTRMYIYTNAGQAHCFNTQCGYIQASIDIPVDLVLEPVSTYGGQPYGITLSIYQDTINLNWYLKYDNNRTVIGWWPSRIFTNLGSTATGAEWGGEVFSPPNVPSPAMGSGHRIRLDTNYDAFFAAANIVVNNTIIKPPKVLKLFDDSSHFDVINKADVGGDPGYLILYGGVPGATGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AG85</accession>
    <name>A0AA39AG85_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Plasma membrane ATPase</fullName>
            <ecNumber evidence="15">7.1.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_002108</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>ATP + H2O + H(+)(in) = ADP + phosphate + 2 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:20852"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Cell membrane</location>
            <topology evidence="15">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIIA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.1" evidence="15"/>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AG85"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008553">
        <property type="term" value="F:P-type proton-exporting transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120029">
        <property type="term" value="P:proton export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02076">
        <property type="entry name" value="P-type_ATPase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000004">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000004">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000211">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006534">
        <property type="entry name" value="P-type_ATPase_IIIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01647">
        <property type="entry name" value="ATPase-IIIA_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42861">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 15" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="12 15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 15" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="15" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="247"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="281"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="685"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="720"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="753"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="16">
        <location>
            <begin position="17"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU362083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9706982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="883" mass="96639" checksum="4A46547FC67135DF" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AGH5</accession>
    <name>A0AA39AGH5_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_001896</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AGH5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47950:SF6">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47950">
        <property type="entry name" value="CYTOCHROME P450, FAMILY 76, SUBFAMILY C, POLYPEPTIDE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9706647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="118" mass="13182" checksum="FB6B5046DFAFB745" modified="2024-01-24" version="1">MLEVVWQELVVAGTGTGRVTAEWAMAVSMKKIREELTTEINQNTLKDSDLQKLPYLQACLKETLRLHPPGPLLLPSTVDFQGNNFELIPYSSGKENLPWTSHGCKTDSLCYGLLDSFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AH29</accession>
    <name>A0AA39AH29_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_002182</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AH29"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.330:FF:000001">
        <property type="entry name" value="Protein SRG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050295">
        <property type="entry name" value="Plant_2OG-oxidoreductases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47991">
        <property type="entry name" value="OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="6">
        <location>
            <begin position="194"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9707074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="356" mass="40209" checksum="D2D030C7E2AE7392" modified="2024-01-24" version="1">MVGSVPVANVQALASSYSGDLPLRYLRPELHAEEVLVDKSLQIPTIDMRKLLVDDDEMGKLHLACKEWGFFQLINHGAAEEVIEKMKADVQEFFKLPLKEKNAYAKLPNGVEGYGQNFVVSEDQKLDWADMHFLQSLPTSERNMRFWPEEPTSFRGTLEKYSLELVKVSNCLLKLMAKNLLINPDQLTNMFDDGRQAVRMNYYPPCVHASKVIGLTPHSDFGGLTLLVQVNEVQGLQIKRNGKWIPIRPVPGAFIVNIGDTIEIMSNGEYKSIEHRAVVDPEKERLSIATFCSPNAGAIIGPLPELTKEKGAIYKSVSREEYIKFVLGRKLDGKSTINHMKLENWKAMITMCCRKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AHZ3</accession>
    <name>A0AA39AHZ3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box associated beta-propeller type 3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_000141</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9707927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AHZ3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013187">
        <property type="entry name" value="F-box-assoc_dom_typ3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017451">
        <property type="entry name" value="F-box-assoc_interact_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01640">
        <property type="entry name" value="F_box_assoc_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31111">
        <property type="entry name" value="BNAA05G37150D PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31111:SF138">
        <property type="entry name" value="F-BOX ASSOCIATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08268">
        <property type="entry name" value="FBA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box associated beta-propeller type 3" evidence="1">
        <location>
            <begin position="51"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9707927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="347" mass="39684" checksum="939F7D4BCCE957F1" modified="2024-01-24" version="1">MISDPSFVEAHQSRSATTLLISFPDMRRPRGRRHLFSISDGEARQLSGFPHWNSSQSVNGLICIYEQLVRSSPKLSFRVIVCNPSTRERVTLPPTHFAFYHQNISLGFDPSTKTYKILRAWWGSYRGTMYEIFTLGSHAWRIIKDGPEFASETKGICLNGTIYWAAAFDLSEDDSNLVVMENRVIAFDVGEEKFRSVPVPPEAPIWDKYKSNIIQIGGHMAIADCQEVATSISTAMVVWKLEDSVNGVWSQKRILLPECWIHRPVPNPRFRRFFVASSDGDKIILIPSGFFRDFSVFQCNIEEGCLWREAIHRPPQDDWVSRSSIVISRQVCEYVESLVSLKEICRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ALL9</accession>
    <name>A0AA39ALL9_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_001419</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ALL9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9709926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="122" mass="13680" checksum="33D0A7C721CC83D6" modified="2024-01-24" version="1">MVEPAAKGLPSLTRQSSTARRLNILGFTRTAEANIPHFKWRFEMEQPRAHAFVISTLKAGIPLGRVPALDCAITISPFEQFVSAATTGQRHCKLKIYIIYIFSISYLLPSFLASRLACWLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D4R8</accession>
    <name>A0AA39D4R8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ACT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_026448</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the acetolactate synthase small subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9669880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D4R8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003984">
        <property type="term" value="F:acetolactate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990610">
        <property type="term" value="F:acetolactate synthase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04878">
        <property type="entry name" value="ACT_AHAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1150:FF:000001">
        <property type="entry name" value="Acetolactate synthase small subunit"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.260:FF:000001">
        <property type="entry name" value="Acetolactate synthase, small subunit"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1150">
        <property type="entry name" value="ACT-like. Chain A, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004789">
        <property type="entry name" value="Acetalactate_synth_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027271">
        <property type="entry name" value="Acetolactate_synth/TF_NikR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019455">
        <property type="entry name" value="Acetolactate_synth_ssu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039557">
        <property type="entry name" value="AHAS_ACT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054480">
        <property type="entry name" value="AHAS_small-like_ACT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00119">
        <property type="entry name" value="acolac_sm"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008864">
        <property type="entry name" value="PRK11895.1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30239">
        <property type="entry name" value="ACETOLACTATE SYNTHASE SMALL SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30239:SF18">
        <property type="entry name" value="ACETOLACTATE SYNTHASE SMALL SUBUNIT 2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22629">
        <property type="entry name" value="ACT_AHAS_ss"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10369">
        <property type="entry name" value="ALS_ss_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACT" evidence="6">
        <location>
            <begin position="78"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="ACT" evidence="6">
        <location>
            <begin position="311"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9669880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="479" mass="52772" checksum="A7054071DFFA6373" modified="2024-01-24" version="1">MAAVAAYPNGFTTLKHDALRVELNGGSLGVLGFGGTLRRLRPKSVTRFEVERQLQVCASTEKAVSAPPIQRSKVKRHTISVFVGDESGIINRIAGVFARRGYNIESLAVGLNEDKALFTIVVSGTETVLRQVVEQLNKLVNVLKVKDLSREPQVERELMLIKLNADPSKRAEIMWLVDIFRAKIVDISEHSLTIEVTGDPGKMVAVQRNLSKFGIKELARTGKIALRREKMGETAPFWRFSAASYPDLEETIPVNALVRNVKRPLNGNPSTSPGGDVYPVEPFNDFAVNQVLDPHWGALNDEDSSGLRSHTLSMLVNDTPGVLNIVTGVISRRSYNIQSLAVGPAEMEGRSRITTVVPGTDESISKLVQQLQKLIDLHEVRDITHMPFAERELMLIKIAVNTAARRDVLDIASIFRAKAVDVSDHTITLELTGDLNKMVALQRLLEPYGICEVARTGRVALVRESGVDSTYLRGYALPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D5M0</accession>
    <name>A0AA39D5M0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Chlororespiratory reduction 41</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_025579</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D5M0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053351">
        <property type="entry name" value="Chloroplast_NDH_Assembly"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36719">
        <property type="entry name" value="OS01G0676200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36719:SF1">
        <property type="entry name" value="PROTEIN CHLORORESPIRATORY REDUCTION 41, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9671996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="241" mass="27812" checksum="AB150C5C7F7A896D" modified="2024-01-24" version="1">MASTFLRFITHVNPLLLHHNQPSQTSNTLLRIHHLPTPKPFTVNCTTPPSSPDPPDFPNPYPNSDTTNNAETFPIEKRRKSEIIRDRKARTQLEKQEPPNFEVGWKRSKVIELEKPKGYVIADFLEKLEELMGREFGSTELLAKTGEIVADRAREEAEVLRDEGEVDERLVTELFRVLRLMEMDLAMVKAAVKEETLSERLEQAKARNKCWRVFNSLLPSQIFRSFEASPSFFLPFTEDFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D601</accession>
    <name>A0AA39D601_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_023059</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D601"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37213">
        <property type="entry name" value="SUBTILISIN-LIKE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37213:SF1">
        <property type="entry name" value="SUBTILISIN-LIKE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9673223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="97" mass="10934" checksum="5743C1F52D15839E" modified="2024-01-24" version="1">MASNWRRSLGNMRSFIGNCMGGLRGGSNLASWVVAGTLAYFLWVKPSQELRREQEERAALAAASDPYRYVEKRKPIPDPQETGLIYGNKNRTNKSQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D697</accession>
    <name>A0AA39D697_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_025337</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Di19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D697"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033347">
        <property type="entry name" value="Di19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027935">
        <property type="entry name" value="Di19_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008598">
        <property type="entry name" value="Di19_Zn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31875">
        <property type="entry name" value="PROTEIN DEHYDRATION-INDUCED 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31875:SF6">
        <property type="entry name" value="PROTEIN DEHYDRATION-INDUCED 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14571">
        <property type="entry name" value="Di19_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05605">
        <property type="entry name" value="zf-Di19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Di19 zinc-binding" evidence="2">
        <location>
            <begin position="40"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="Di19 C-terminal" evidence="3">
        <location>
            <begin position="114"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9671575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="225" mass="25445" checksum="D1E28A2E74A80F13" modified="2024-01-24" version="1">MDSDHWSSRLAVAKRQYMLHQTNKSHSDRLCIDDLEVEDDIPCPYCYEDHDIASLCSHLEDEHSFESRVAVCPICSVKIARDMLGHITMQHRHLFKLQRRRRLRRVAIPNSQALSLLGRDLREAHLQVLLGGGGYRPSNANASNSLTESLLSSLALNFPLCEAEEITKSVVPDLEDISGANSSKKTWNTSVYSSLSSEEREKKIRQTTARSGFVQDLLISTMFDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D870</accession>
    <name>A0AA39D870_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_024387</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D870"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9675443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="120" mass="13367" checksum="5A2C0710A7C0F6D3" modified="2024-01-24" version="1">MSTPLEAKTRTSSNNVLLEDPIYFRRLAADLFTKPMSKAALNNFRTKLCLQPRHSLREGIGTTQHLGVNCETQRSDKVEHNYGSDKVENSHNSCYNSGETLKDEGVSMATTLTRNDSSTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D9K0</accession>
    <name>A0AA39D9K0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">Laccase</fullName>
            <ecNumber evidence="4 13">1.10.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Benzenediol:oxygen oxidoreductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Diphenol oxidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Urishiol oxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_024577</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Lignin degradation and detoxification of lignin-derived products.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>4 hydroquinone + O2 = 4 benzosemiquinone + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:11276"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17594"/>
            <dbReference type="ChEBI" id="CHEBI:17977"/>
            <dbReference type="EC" id="1.10.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="13">Binds 4 Cu cations per monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Secreted</location>
            <location evidence="2 13">Extracellular space</location>
            <location evidence="2 13">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the multicopper oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.10.3.2" evidence="4 13"/>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D9K0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052716">
        <property type="term" value="F:hydroquinone:oxygen oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046274">
        <property type="term" value="P:lignin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13875">
        <property type="entry name" value="CuRO_2_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13897">
        <property type="entry name" value="CuRO_3_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033138">
        <property type="entry name" value="Cu_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002355">
        <property type="entry name" value="Cu_oxidase_Cu_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034285">
        <property type="entry name" value="CuRO_2_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034289">
        <property type="entry name" value="CuRO_3_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017761">
        <property type="entry name" value="Laccase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03389">
        <property type="entry name" value="laccase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF487">
        <property type="entry name" value="LACCASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00079">
        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00080">
        <property type="entry name" value="MULTICOPPER_OXIDASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0052">Apoplast</keyword>
    <keyword evidence="10 13" id="KW-0186">Copper</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 13" id="KW-0439">Lignin degradation</keyword>
    <keyword evidence="7 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 13" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Plastocyanin-like" evidence="16">
        <location>
            <begin position="2"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="14">
        <location>
            <begin position="72"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="15">
        <location>
            <begin position="351"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9675714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="480" mass="53681" checksum="C0CDF9D68BDFF98B" modified="2024-01-24" version="1">MPRYPWTDGPEYITQCPIQPGSKFSQKIILSSEEGTIWWHAHSDWTRATVHGAIIVYPNNGTKYPFHKPNAEFLIILGQWWKSDVNAVRDKGLATGADPDPSDALLINGQPGDLYPCSKSSTFKLTVDHGKTYLLRIINAALQEALFFAIAKHKMIVVGTDGSYTKPLTRDYITIFPGQTFDVLLEANQRPDHYYMAAITYSVAPKYQNFYDNTTTTAIVQYRGYYTPSSPHSLPHLPAYNNTNASVQVMASLRSLANAEHPCNVPLSTSTNLIYTVSVNTYPCINNSCAGTNGTRFSSSINNISFDSPTIDILEAYYYNISGVYGDKFPSVPPLVFDFTADYLPLVYELPSRGTEVRVLEYNSTVELVLQGTTVVTGTHHPMHLHGHSFYLVGWGFGNFNENRDPLRYNLVDPPLQNTISVPSEGWVAIRFEASNPGVWFLHCHVERHQTWGMKTAFIVKNGKHPKAQMLPPPSDMPPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DA69</accession>
    <name>A0AA39DA69_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Translation elongation factor EF1B beta/delta subunit guanine nucleotide exchange domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_021926</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">EF-1 is composed of 4 subunits: alpha, beta (1B-alpha=beta'), delta (1B-beta), and gamma (1B-gamma).</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the EF-1-beta/EF-1-delta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9676646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DA69"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005853">
        <property type="term" value="C:eukaryotic translation elongation factor 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00292">
        <property type="entry name" value="EF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.60:FF:000001">
        <property type="entry name" value="Elongation factor 1-beta 1 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.130:FF:000006">
        <property type="entry name" value="Elongation factor 1-delta 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036219">
        <property type="entry name" value="eEF-1beta-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049720">
        <property type="entry name" value="EF1B_bsu/dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014038">
        <property type="entry name" value="EF1B_bsu/dsu_GNE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014717">
        <property type="entry name" value="Transl_elong_EF1B/ribsomal_bS6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001326">
        <property type="entry name" value="Transl_elong_EF1B_B/D_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11595">
        <property type="entry name" value="EF-HAND AND COILED-COIL DOMAIN-CONTAINING FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11595:SF21">
        <property type="entry name" value="ELONGATION FACTOR 1-BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00736">
        <property type="entry name" value="EF1_GNE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00888">
        <property type="entry name" value="EF1_GNE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54984">
        <property type="entry name" value="eEF-1beta-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00824">
        <property type="entry name" value="EF1BD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00825">
        <property type="entry name" value="EF1BD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="233"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="277"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="Translation elongation factor EF1B beta/delta subunit guanine nucleotide exchange" evidence="9">
        <location>
            <begin position="141"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="88"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9676646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="305" mass="33752" checksum="2A0A5E68E70D8BC2" modified="2024-01-24" version="1">MAVTFYGVNSNSGLKKLDEYLLTRSYISGYQASKDDITVHAALSKAPSSEYVNVSRWYNHIEALLRISGVTGEGCGVTIEGFAPAESVATPPAEDTKAAAAEDDDDDDVDLFGEETEEEKKAAEARAAAVKASGKKKESGKSSVLLDVKPWDDETDMKKLEEAVRSIQMEGLFWGASKLVPVGYGIKKLQIMLTIVDDLVSVDDLIEERLTAEPTNEYVQSCDIVAFNKICKFIFMLCGIGIKPYPLLCSVLCFMSVNSCSDSSDLCLQNLRIRPRFWGVHYCRMIIILSFPFIFLQDMFRVSVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DAL4</accession>
    <name>A0AA39DAL4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S ribosomal protein L13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_024269</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DAL4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000003">
        <property type="entry name" value="60S ribosomal protein L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00499">
        <property type="entry name" value="Ribosomal_eL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001380">
        <property type="entry name" value="Ribosomal_eL13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018256">
        <property type="entry name" value="Ribosomal_eL13_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11722">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11722:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01294">
        <property type="entry name" value="Ribosomal_L13e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01104">
        <property type="entry name" value="RIBOSOMAL_L13E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9675247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="207" mass="23891" checksum="99CAECC8CE6CA0BC" modified="2024-01-24" version="1">MVKHNNVVPNGHFKKHWQNYVKTWFNQPARKTRRRIARQKKAVKIFPRPTSGPLRPIVHGQTLKYNMKVRAGRGFSLEELKAAGIPKKLAPTIGIAVDHRRKNRSLESLQANVQRLKTYKAKLVVFPRRTRKFKAGDSSVEELATATQVQGLYMPIVREKPTVELVKVTDEMKSFKAYGKLRVERMNQRHVGARMKKAAEAEKEEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DAW6</accession>
    <name>A0AA39DAW6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UDENN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_022196</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DENND6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DAW6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024224">
        <property type="entry name" value="DENND6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037516">
        <property type="entry name" value="Tripartite_DENN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13677">
        <property type="entry name" value="LD41638P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13677:SF0">
        <property type="entry name" value="LD41638P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50211">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDENN" evidence="2">
        <location>
            <begin position="36"/>
            <end position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9677079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="683" mass="76930" checksum="F21A8104791A9908" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DC37</accession>
    <name>A0AA39DC37_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Maltose excess protein 1-like, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_020464</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DC37"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009941">
        <property type="term" value="C:chloroplast envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005363">
        <property type="term" value="F:maltose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034628">
        <property type="entry name" value="MEX1/MEX1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34809">
        <property type="entry name" value="MALTOSE EXCESS PROTEIN 1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34809:SF1">
        <property type="entry name" value="MALTOSE EXCESS PROTEIN 1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="129"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="160"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="189"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="222"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="246"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="276"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="307"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="337"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="366"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9678309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="405" mass="44445" checksum="8115B5E4A2ABCF88" modified="2024-01-24" version="1">MVVVGLGVGVGVGSSLPSGTAGWSPLFHSSLRQKPLTHVKHVGNRFHFSPSLSSFPRRLPRLPPVLALDSDLPRPVHRRSEKLKSSKSFEQWDSLTAKFSAGSNLPFLLLQLPQIILNARNLLAGNKSALFAVPWLGMLTGLLGNISLLSYFVKKREAEAVVVQTLGVVSIYAVIVQLAMAGAMPLPHFTVTSIVVASGLVLNFLFYFGLLNSTLWELWQDFITIGGVSAFPQVIWSTLVPFIPNSILPGAIAFVIGLVAVVMARTGKLPEKGVKFVRAISGWTATLLFMWMPVAQMMTNFLSPENIKGLSAFSMLLAMIGNGLMIPRALFIRDFMWFLGSSWASIFYGWGNLICLYYFNIISREFFLAATVGLYAWIGVAFWRDTKVYGYDSPLASMKELVSGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DDE2</accession>
    <name>A0AA39DDE2_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_021741</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DDE2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025322">
        <property type="entry name" value="PADRE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33148:SF2">
        <property type="entry name" value="DUF4228 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33148">
        <property type="entry name" value="PLASTID MOVEMENT IMPAIRED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14009">
        <property type="entry name" value="PADRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9679931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="151" mass="16469" checksum="084284320D7F4EC8" modified="2024-01-24" version="1">MGNCFALCIPSKRRVLWVVKRDGKMIQFRGPILVKDVLVNFSGLGVGVSREALKHLPSDYELKIGKVYYLLPCPCSSGGSVGAPDVSSMADDGGQKGSGMKRIKVVITKQQLQDLLSKQISLEELLLSQSVDSSSPRNWKPKLDPIPEGIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DDQ5</accession>
    <name>A0AA39DDQ5_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_020614</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DDQ5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015495">
        <property type="entry name" value="Myb_TF_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10641">
        <property type="entry name" value="MYB FAMILY TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10641:SF1103">
        <property type="entry name" value="TRANSCRIPTION FACTOR MYB72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="4">
        <location>
            <begin position="3"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="5">
        <location>
            <begin position="57"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="4">
        <location>
            <begin position="57"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9678472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="145" mass="17627" checksum="4B1427C589733DBF" modified="2024-01-24" version="1">MTRPPKTRRRWTREEDDALKYFKTEYPNLPWGKIIEQAKLQDRDEKSCSRRWNDHLRPDINKSEFSPQEDELIIHLKSLGVSWASMRKEHLLERRPSSDIKNRWHNHLKKRTVTVSDPRRPLPNHVQQPLMNDQTGLDIFFDYRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DGS1</accession>
    <name>A0AA39DGS1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_017827</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleoporin GLFG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9681607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DGS1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044614">
        <property type="term" value="C:nuclear pore cytoplasmic filaments"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008139">
        <property type="term" value="F:nuclear localization sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000973">
        <property type="term" value="P:post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006405">
        <property type="term" value="P:RNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034398">
        <property type="term" value="P:telomere tethering at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2360:FF:000001">
        <property type="entry name" value="Nuclear pore complex protein Nup98-Nup96"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037665">
        <property type="entry name" value="Nucleoporin_S59-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23198:SF6">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP98-NUP96"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23198">
        <property type="entry name" value="NUCLEOPORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="7" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0811">Translocation</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9681607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="227" mass="23779" checksum="EF61F9B6BAC1A7D8" modified="2024-01-24" version="1">MSAFGTSSMPGFGSSTRPPFEASSTIAFGFCSTPLFGQPTSTFDVNAVGANPFPYGTQSSHFGPQITASTVGSMGFGKLASSDPSGGSRIAAYAATAEVDGTNFEQPAGKLKSISAVPVYRNKSHEELRWEDYQLGDKGGSNPFCTPNLGSGGPYGASTVSAFGSSSMLAFGLSSTLALRFGSTHPLTNQAMHLLAAQLELIHLLMEHRVPILDHRLQNQHLEAQGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DGU9</accession>
    <name>A0AA39DGU9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Expansin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_018922</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Causes loosening and extension of plant cell walls by disrupting non-covalent bonding between cellulose microfibrils and matrix glucans. No enzymatic activity has been found.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Secreted</location>
            <location evidence="7">Cell wall</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Membrane</location>
            <topology evidence="7">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the expansin family. Expansin A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DGU9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009664">
        <property type="term" value="P:plant-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22274">
        <property type="entry name" value="DPBB_EXPA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.760">
        <property type="entry name" value="Expansin, cellulose-binding-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007118">
        <property type="entry name" value="Expan_Lol_pI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002963">
        <property type="entry name" value="Expansin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007112">
        <property type="entry name" value="Expansin/allergen_DPBB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007117">
        <property type="entry name" value="Expansin_CBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036749">
        <property type="entry name" value="Expansin_CBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009009">
        <property type="entry name" value="RlpA-like_DPBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31867">
        <property type="entry name" value="EXPANSIN-A15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03330">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01357">
        <property type="entry name" value="Expansin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01226">
        <property type="entry name" value="EXPANSIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01225">
        <property type="entry name" value="EXPANSNFAMLY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00837">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49590">
        <property type="entry name" value="PHL pollen allergen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50843">
        <property type="entry name" value="EXPANSIN_CBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50842">
        <property type="entry name" value="EXPANSIN_EG45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0134">Cell wall</keyword>
    <keyword evidence="6 7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041482955" description="Expansin" evidence="7">
        <location>
            <begin position="25"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like EG45" evidence="8">
        <location>
            <begin position="46"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like CBD" evidence="9">
        <location>
            <begin position="168"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9683125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="251" mass="27910" checksum="2737DBB6DCEECEDE" modified="2024-01-24" version="1">MAGSHFLLGGVMFTTLLLLGLASSQRGWYDAHATFYGDMQGNEPMQGDCGYGDLFKQGYGLETTALSTALFDNGLSCGACFEIKCVNDPQWCIPDARTITVTATNFCPPNYSKPTGGWCNPPRQHFDLSMPMFVRIAKYKAGIIPVQYRRVRCHKRGGIKFEIKGNRYWVLVLVFNVGGVGDVSNVKIKGSNTQWLQMSRNWGQNWDVGVVLVGQSLSFQVTTRDQKMVQSDNVAPADWQFGQTFEGKSNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DP53</accession>
    <name>A0AA39DP53_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Prolamin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_014661</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9689122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DP53"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036312">
        <property type="entry name" value="Bifun_inhib/LTP/seed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040220">
        <property type="entry name" value="DD11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008502">
        <property type="entry name" value="Prolamin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31207">
        <property type="entry name" value="ECA1 GAMETOGENESIS FAMILY PROTEIN (DUF784)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31207:SF35">
        <property type="entry name" value="PROLAMIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05617">
        <property type="entry name" value="Prolamin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47699">
        <property type="entry name" value="Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041296010" description="Prolamin-like domain-containing protein" evidence="2">
        <location>
            <begin position="25"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Prolamin-like" evidence="3">
        <location>
            <begin position="68"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9689122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="150" mass="16043" checksum="67723954415FEC3D" modified="2024-01-24" version="1">MASFGNVYTMGLILLASVAVMALGEVIEDDVGFIPTASPGPSGIEYDAPDTDPSTQSILPFYQYLKACVQKVPTGCGPQIFSYLFKDGPAIASHCCEQLGSVGIDCHESLTLYLVGIPEFSGKEDHIISKAKELIEKCPRKNRFAAPPSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DPW0</accession>
    <name>A0AA39DPW0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_011712</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DPW0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052422">
        <property type="entry name" value="Auxin_Ser/Thr_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47986">
        <property type="entry name" value="OSJNBA0070M12.3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47986:SF29">
        <property type="entry name" value="RECEPTOR PROTEIN KINASE TMK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="1"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9692756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="175" mass="19999" checksum="8C5FF402DE0D2DA1" modified="2024-01-24" version="1">MGVPIWVFILASTLEIPSVVGIRFWMRTYSILVIFFSVHGSYIHYQLVVGLDVARGVEYLLGSAHQSFIHRDLKPSDILLKDNMRAKIADRGLALLVPDENSSFETRIVGTLVLEYVLTGRVTTKVDVTSFGVILMELITRRKALDESQPKENMRLVTWYKRRHINMDTFRIASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DTZ7</accession>
    <name>A0AA39DTZ7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_008355</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DTZ7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010105">
        <property type="term" value="P:negative regulation of ethylene-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000867">
        <property type="entry name" value="ETO1-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044631">
        <property type="entry name" value="ETO1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44203:SF2">
        <property type="entry name" value="ETO1-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44203">
        <property type="entry name" value="ETO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13176">
        <property type="entry name" value="TPR_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="BTB" evidence="4">
        <location>
            <begin position="179"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="696"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="748"/>
            <end position="775"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9696054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="873" mass="98515" checksum="8EEEC4CD56924B0B" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DX40</accession>
    <name>A0AA39DX40_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_009114</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DX40"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14798">
        <property type="entry name" value="RX-CC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038005">
        <property type="entry name" value="RX-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF1052">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RPM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="5">
        <location>
            <begin position="5"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="4">
        <location>
            <begin position="174"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="6">
        <location>
            <begin position="422"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="7">
        <location>
            <begin position="539"/>
            <end position="850"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9697192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="865" mass="97919" checksum="99CC9F59F0E26499" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DYI2</accession>
    <name>A0AA39DYI2_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_004486</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DYI2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15852:SF66">
        <property type="entry name" value="OS09G0423700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15852">
        <property type="entry name" value="PLASTID TRANSCRIPTIONALLY ACTIVE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="DnaJ/Hsp40 cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9702776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="147" mass="15794" checksum="736BE1C619643A82" modified="2024-01-24" version="1">MSTCVCCCRPAILGKSKEGVSNGVVLKPPGSISSSRSMNSRVFGVRASMVDSYESSSNFAKRMEQAWLISQQPRPVSCTSCETKGHVECKWCRGTGFFILGDNMLCQVPSRNTSCVICAGKGSTCCSDCKGTGFRAMWLGEPPVSKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E0M5</accession>
    <name>A0AA39E0M5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_005138</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E0M5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF78">
        <property type="entry name" value="OS12G0587133 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9703718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="578" mass="66514" checksum="621B0CB68664F44A" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E1Q7</accession>
    <name>A0AA39E1Q7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutaredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_001736</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E1Q7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03031">
        <property type="entry name" value="GRX_GRX_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45669">
        <property type="entry name" value="GLUTAREDOXIN DOMAIN-CONTAINING CYSTEINE-RICH PROTEIN CG12206-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45669:SF22">
        <property type="entry name" value="GLUTAREDOXIN DOMAIN-CONTAINING CYSTEINE-RICH PROTEIN CG12206-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23733">
        <property type="entry name" value="GRXCR1-2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutaredoxin" evidence="1">
        <location>
            <begin position="164"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9706361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="290" mass="31598" checksum="C2D35F5D0A2533B4" modified="2024-01-24" version="1">MGCVSSTLLNHEEELGQLSGSTLGHHIVSLTSTTYGLLNLDPPPQPTHPPTPTPRFTLGSVFPSPLSEPRGIRSEPEVINSWELMAGLDADSFRFPPLPLAPSKPKALAIKETNINKENSNPNRPNCGDESRVLKPLNDSVLRQCLALLDGFERLCPPNGENRVVVYTTTLRGVRKTFEDCNAVRAAIEGLGVSLCERDISMDRGFKEELRELMKGKDSGQMVPPRVFVKGRYVGGAEEVLRIVEEGCLSELLQGLPKIRAGEVCEGCGGVRFLPCFQCNGSCKMGGVLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E1T2</accession>
    <name>A0AA39E1T2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_003968</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9701992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E1T2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000170">
        <property type="entry name" value="Alpha/beta-Hydrolases superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42886:SF38">
        <property type="entry name" value="ALPHA_BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42886">
        <property type="entry name" value="RE40534P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="1">
        <location>
            <begin position="44"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9701992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="295" mass="32905" checksum="9F5A0AA1C8B4B89D" modified="2024-01-24" version="1">MSVTRPSPTHLERIGPPVIKQQRVVIQNQHGEKLVGISHETGSKELVILCHGFRSSKEHIPMVSLAAALGKEGISAFRFDFSGNGESEGSFQYGNYRREADDLRAVVQHFYGENRVIIALVGHSKGGNVVLLYASKYNDVHTIVNISGRFYLERGIKGRLGQDFLQRIKQNGFIDVKNKGGKFQYRVTEESLMDRLTTDTHATCLLIQKDCRVLTVHGSCDEIVPMEDALAFAKIIPNHKLHIIEGADHEFTSHQDELASVVLDFVRSYPPCSKGISEQLPPSCIRADNSTHSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E3F2</accession>
    <name>A0AA39E3F2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_005109</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E3F2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.150.80:FF:000003">
        <property type="entry name" value="NAC domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.80">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003441">
        <property type="entry name" value="NAC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036093">
        <property type="entry name" value="NAC_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31744:SF236">
        <property type="entry name" value="NAC DOMAIN-CONTAINING PROTEIN 105"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31744">
        <property type="entry name" value="PROTEIN CUP-SHAPED COTYLEDON 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02365">
        <property type="entry name" value="NAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101941">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51005">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NAC" evidence="6">
        <location>
            <begin position="9"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9703672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="336" mass="38682" checksum="4115026537F58337" modified="2024-01-24" version="1">MMDSMESCVPPGFRFHPTDEELVGYYLKKKVASQKIDLDVIRDIDLYRIEPWDLQERCRIGYEEQNEWYFFSHKDKKYPTGTRTNRATMAGFWKATGRDKAVYDKTKLIGMRKTLVFYKGRAPNGQKTDWIMHEYRLESEENGPPQAKGWVVCRAFKKRTTSQSKSIEGWDTSYFYDEPSGVSTVMDPIDYISRQPQNYLGQNFLCKQEIEADNLNFMHATEHFVQLPQLESPSLPLMKRPSSVSLISENNEEEDQTRGCNNAEKVTDWRALDKFVASQLSQEDTYEGDGVSGFGAHHNSDMALLLLQSSRDEANKLNGFLNSSSDCDIGICVFEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E4K1</accession>
    <name>A0AA39E4K1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NAD(P)H dehydrogenase (quinone)</fullName>
            <ecNumber evidence="3">1.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_003069</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a quinone + NADH + H(+) = a quinol + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:46160"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.6.5.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a quinone + NADPH + H(+) = a quinol + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:46164"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.6.5.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WrbA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.6.5.2" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E4K1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003955">
        <property type="term" value="F:NAD(P)H dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.360:FF:000001">
        <property type="entry name" value="NAD(P)H dehydrogenase (Quinone) FQR1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010089">
        <property type="entry name" value="Flavoprotein_WrbA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005025">
        <property type="entry name" value="FMN_Rdtase-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01755">
        <property type="entry name" value="flav_wrbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002999">
        <property type="entry name" value="PRK03767.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30546">
        <property type="entry name" value="FLAVODOXIN-RELATED PROTEIN WRBA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30546:SF24">
        <property type="entry name" value="NAD(P)H DEHYDROGENASE (QUINONE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03358">
        <property type="entry name" value="FMN_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="7">
        <location>
            <begin position="52"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9704842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="256" mass="27472" checksum="55BA218ED4EB8E28" modified="2024-01-24" version="1">MGKGGGCVLSKKRMPLPVSDDPPNEDTAIPVPLLDTAIPEAGDLNSPSKLRIFIVFYSMYGHVEILARRIKAGVDSIDGVEGVLFRIPETMPPEVLEQMKVPQKADDVPVMSEAAELVEADGLLFGFPTRYGSMAAQMKAFFDSTGQLWREQKLAGVPAGFFVSTGTQGGGQETTALTAITQLAHHGMLYVPIGYTFGAGMFKMDSIRGGSPYGAGVFSGDGTREPSETELALAEHQGKYMAAIVKRFARPRTLEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E527</accession>
    <name>A0AA39E527_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_002419</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0587 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E527"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008584">
        <property type="entry name" value="CXXC_Zn-binding_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12857">
        <property type="entry name" value="CXXC MOTIF CONTAINING ZINC BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12857:SF0">
        <property type="entry name" value="CXXC MOTIF CONTAINING ZINC BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05907">
        <property type="entry name" value="CXXC_Zn-b_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141678">
        <property type="entry name" value="MAL13P1.257-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9707394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="163" mass="18528" checksum="31260DB5CEEE54D5" modified="2024-01-24" version="1">MPKLMLVVSAELKNIVILQPEDGSDDEKINYYFKLECEHCGWVCENEACVSINVKEKPTGNRRNDNLNLSRKCHGCERYGGISLIPGHGAPLSQNHCTEVDLMLFNYDGLLHVEYSFNVFLYFFQTSGRKIHVDLAMGEYTDVIDGVEVSISKLKAKFVLYWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E7I5</accession>
    <name>A0AA39E7I5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF4283 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_002164</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E7I5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="150"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="201"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="285"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="207"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9707047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="576" mass="63920" checksum="6A9659443B8F68C9" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39EAD2</accession>
    <name>A0AA39EAD2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_000517</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39EAD2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23807">
        <property type="entry name" value="UEV_UBE2V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000026">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="2">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9708517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="124" mass="14200" checksum="D8293FC5036016A3" modified="2024-01-24" version="1">MDDADDIYMQSWTGTIIGPPNTVHEGRIYQLKLFCGQDYPDNPPAVKFQTRINMTCVNQETGVVEPSLFPMLATWQREHTMEDILTQLKKEMMSPQNRKLAQPPEGNEEARMDQKGLVLKCCIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ECW2</accession>
    <name>A0AA39ECW2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PROP1-like PPR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_001164</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ECW2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050667">
        <property type="entry name" value="PPR-containing_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033443">
        <property type="entry name" value="PROP1-like_PPR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47939">
        <property type="entry name" value="MEMBRANE-ASSOCIATED SALT-INDUCIBLE PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47939:SF11">
        <property type="entry name" value="TETRATRICOPEPTIDE-LIKE HELICAL DOMAIN SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17177">
        <property type="entry name" value="PPR_long"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PROP1-like PPR" evidence="4">
        <location>
            <begin position="112"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="166"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="197"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="232"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="267"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF17177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9709552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="386" mass="44165" checksum="5420533EBDD4283A" modified="2024-01-24" version="1">MASLCRIPRRLLSLARFSTLSDPFTTFLAAKSAVESEPDPEKLAHIFHHQSSNFARFRRHRPLYQLSCRRLSRSGRLDLVERLIDHQKTLPHPRTEGFWIRLIMLYSTSGMVDHALRTFHQMVQDRVQLTEKSLCAILTVYLDNDLIDQLHTVFNTMPSEIGVSPGTKAYSLVLKAFCRQKDMESARKLLHKMENSDIGSYNVLLEGYSKNGDGVEFDEILKEIKNKGLEHDCTTYNHRILRFCKNKESVRAKKLLDEMVAKGVKPNSASYNMIIHGFCKVGDFESAQKVLERMLADGYVAPCSISYITLFQHMVKEGEFDPALNMCKEIIRRKWVPPFEAMDGLVKGLVEMSKVEAAKEVVEKMKKRLKGNATDSWKTHEAALPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YHS3</accession>
    <name>A0AA38YHS3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HXXXD-type acyl-transferase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_026932</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YHS3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051283">
        <property type="entry name" value="Sec_Metabolite_Acyltrans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31896">
        <property type="entry name" value="FAMILY REGULATORY PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G14730)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31896:SF43">
        <property type="entry name" value="PROTEIN ENHANCED PSEUDOMONAS SUSCEPTIBILITY 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9670699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="437" mass="47860" checksum="A1CE882F17F09BAE" modified="2024-01-24" version="1">MAGISIISTTTVVPASQNESTQRIELTPWELQLLLLGSIQQGLLFLKPSTPPQENSSIIDHLKTSLSRTLDSFYPLAGRLGATVNDDNTTSFFINCNGAGAQFVHAAAHDVSVADVLEPAYVPRIVLSFFPLDGVRNSAGVSEPLLAVQVTELVDGIFIGCTVNHMVADGTSFWHFFNSWSEASRSSNNISLPPIFRPWFVDDIHYSIQIPQSALVSAIPTTPPLQEKVFHFTKEKIATLKARANAEMGTHQISSMQALLAHLWRSVIRSRGLAEDQETRYNIPIGIRPRLRPPLPQQYFGVAVQPGTLTMKAGELLELGLGHAAWQVNKTVAAYTEEEATKFLESWVKNPKLLGGRTTMINDSLYMSNSPQFHVYGADFGWGRPVAVRSGGGNKLDGKTTFFPGSEEGSIDIQVCLSPETLEAMMEDAEFMEAVTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YKN7</accession>
    <name>A0AA38YKN7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cytochrome b561 and DOMON domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_025711</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YKN7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08760">
        <property type="entry name" value="Cyt_b561_FRRS1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09629">
        <property type="entry name" value="DOMON_CIL1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045265">
        <property type="entry name" value="AIR12_DOMON"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006593">
        <property type="entry name" value="Cyt_b561/ferric_Rdtase_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005018">
        <property type="entry name" value="DOMON_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017214">
        <property type="entry name" value="UCP037471"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23130">
        <property type="entry name" value="CYTOCHROME B561 AND DOMON DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23130:SF175">
        <property type="entry name" value="CYTOCHROME B561 AND DOMON DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04526">
        <property type="entry name" value="DUF568"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037471">
        <property type="entry name" value="UCP037471"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00665">
        <property type="entry name" value="B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50939">
        <property type="entry name" value="CYTOCHROME_B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50836">
        <property type="entry name" value="DOMON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041394114" description="Cytochrome b561 and DOMON domain-containing protein" evidence="11">
        <location>
            <begin position="31"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="231"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="261"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="298"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="337"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="363"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="DOMON" evidence="12">
        <location>
            <begin position="61"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b561" evidence="13">
        <location>
            <begin position="192"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="398"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="406"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="8">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <label>1</label>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="8">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <label>1</label>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="8">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <label>1</label>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="8">
        <location>
            <position position="335"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <label>1</label>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR037471-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9672185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="456" mass="49665" checksum="88AC2CB7918DD490" modified="2024-01-24" version="1">MYSSNCLKPLLIFPILATLCLLSSTTTSTAQPSTQCSEQFELMITNRSGKNKPKCQKLTTLNAEFGWTILKNKSIDVFFGAWLDNDEGWLAWGVNPGKRPQMIGTRAIIGIKNPNGSLIVNTYNITGGTKLGCQLLPTEFHDNDDVLFSNMKIFTNNTDLVVISATITLPSKYSILDLHHVWQVGDEADGNEPKKHPTALQNVDSTETINLDSGEGHSVGQHRRHLRTVHGILNIVGWGTLLPLGVIVARYFRKFPFESPYWFLVHIYIQTIGYTLGTIGWAIGLVLGHSSRYYTFRIHRILAIFIFTFTSLQMLALRLKPEAKDEYRKYWDMYHHFLGYALLALISVNIFQGIGIFKPDKTWKWAYIGVLGALASITLGFEIYTWVKFLRKKKKDKDTTKQAAGAQGGGGAPSAGGAPGGGGAPSAGGAPGGGGAPGGGGAPHSDDLLSPAGGPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YKZ7</accession>
    <name>A0AA38YKZ7_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_025802</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YKZ7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052234">
        <property type="entry name" value="U5_snRNP_Component"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44006">
        <property type="entry name" value="U5 SMALL NUCLEAR RIBONUCLEOPROTEIN 40 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44006:SF1">
        <property type="entry name" value="U5 SMALL NUCLEAR RIBONUCLEOPROTEIN 40 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="40"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="83"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="131"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="166"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9672329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="239" mass="26633" checksum="189F6634D318D0AE" modified="2024-01-24" version="1">MQGFPSENALSVVGPRPGMELSAVPYTGPRALLESPIMLLASHSSAIYTMKFNPTGIVIASGSYEREIFLCYVHGDCKNFMVLKGHKNVVLDLQWTIGPPLVVNRFDDGIAKLWDMRQTGVIHTFPDKYQITAISFFDASDKIFTSGIDNDVKVWDLRRNEVTMTLQGHQDMITNMQLSQDHSYLITNGMGCKLCIWDMCPYAPQNCCVKILEGGILVDMWTGCIGRASRIIEVADFLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YL50</accession>
    <name>A0AA38YL50_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_025897</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YL50"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000337">
        <property type="entry name" value="ABC transporter G family member 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF135">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="438"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="467"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="509"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="542"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="566"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="659"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="68"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9672462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="686" mass="77831" checksum="1959987B7925EF99" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YL59</accession>
    <name>A0AA38YL59_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_025911</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YL59"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000009">
        <property type="entry name" value="transcription factor MYB1R1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006447">
        <property type="entry name" value="Myb_dom_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01557">
        <property type="entry name" value="myb_SHAQKYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44042:SF15">
        <property type="entry name" value="DUPLICATED HOMEODOMAIN-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44042">
        <property type="entry name" value="DUPLICATED HOMEODOMAIN-LIKE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23082">
        <property type="entry name" value="Myb_DNA-binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Myb-like" evidence="7">
        <location>
            <begin position="13"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="7">
        <location>
            <begin position="99"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="100"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="8">
        <location>
            <begin position="102"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="83"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="154"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="83"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9672484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="184" mass="21287" checksum="097A91BB9681B9AF" modified="2024-01-24" version="1">MSQFIDYSLSFGSKWNRSEDILLERAILIFPEETPNRWYKIVTHIPGKSPMDVLEHYIKLIQDIDAIDFGSMDQYIPDRWDLEEEDQEEEEEGSTGSKVEKKKGTPWTEEEHVLFLEGLVKYGKGDWKSISRNFVITRTPSQVASHAQKYFARQRPGNMGKKRKRTSIHDITTDDLPPLGGVTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YMB1</accession>
    <name>A0AA38YMB1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_026395</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9673095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YMB1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9673095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="355" mass="40891" checksum="0455D5B5EB5723C7" modified="2024-01-24" version="1">MAPKRALAEASSNASEGKSLEVARMIETKGKILDVPKTSDNKQKTPEVRKKATFDRATFTTPELAQRFNLHFANQTVIPGRNIDFAKLSFFQFDKFFTRMDWLPIVSMKEFIYPRWPRVEGFKPAEAIQRLCGYQKSGRPTSHSLTMLSCILQHMISYIFILKGGHRDDVSFLEAFLVDNILIKRNINMGYIIFRHMKACSFSEDSVLPYSMFITKIVKYFNVNLRNETEGKKLKSFDTYDRVSLRHMHFEKEYENQDVEKRGSENANVIEILSNDGVRAMAVADHPSQIGRNEAEASDNLNAQISSIGTHLKEMVLANDRRLTSLEQRIDGFQEEFKVGMQVIRAQYDELMVFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YN57</accession>
    <name>A0AA38YN57_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="25">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">PVL29_023201</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="25">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Serine/threonine-protein kinase that may function as a signaling receptor of extracellular matrix component. Binding to pectin may have significance in the control of cell expansion, morphogenesis and development.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="19">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="25">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000038">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000968">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000084">
        <property type="entry name" value="Wall-associated receptor kinase 2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000043">
        <property type="entry name" value="Wall-associated receptor kinase 2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.20.10">
        <property type="entry name" value="Plasmodium vivax P25 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013695">
        <property type="entry name" value="WAK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045274">
        <property type="entry name" value="WAK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025287">
        <property type="entry name" value="WAK_GUB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27005:SF468">
        <property type="entry name" value="OS01G0310500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27005">
        <property type="entry name" value="WALL-ASSOCIATED RECEPTOR KINASE-LIKE 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13947">
        <property type="entry name" value="GUB_WAK_bind"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08488">
        <property type="entry name" value="WAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 19" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="13 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7 22" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041295336" evidence="22">
        <location>
            <begin position="30"/>
            <end position="1636"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="341"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="709"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="874"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="24">
        <location>
            <begin position="290"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="416"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="24">
        <location>
            <begin position="823"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="949"/>
            <end position="1231"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="1310"/>
            <end position="1592"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="445"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="978"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="1339"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00058961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="KAJ9673497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1636" mass="184168" checksum="4C1435F9746A8FEA" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YRY9</accession>
    <name>A0AA38YRY9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_024396</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YRY9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003906">
        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008311">
        <property type="term" value="F:double-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004808">
        <property type="entry name" value="AP_endonuc_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748">
        <property type="entry name" value="AP ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748:SF4">
        <property type="entry name" value="DNA-(APURINIC OR APYRIMIDINIC SITE) ENDONUCLEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9675452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="143" mass="16420" checksum="95DDBD92DE6BC196" modified="2024-01-24" version="1">MKIISWNTRGLGSRKKRRVVKDFLRLENPDVVMFQETKREVCDRRFVGSVWSVRNKEWVALPACRASGGILIIWDSKKLCNEEVVIGSFSVSVKFLLDGCGPLWLSSVYGPNSSLLRKDFWVELLDIFGLSFLYGVWAMILML</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z1E2</accession>
    <name>A0AA38Z1E2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_019801</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HD-ZIP homeobox family. Class II subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z1E2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000577">
        <property type="entry name" value="Homeobox-leucine zipper protein 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050762">
        <property type="entry name" value="HD-ZIP_Homeobox_LZ_Class_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006712">
        <property type="entry name" value="HD-ZIP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003106">
        <property type="entry name" value="Leu_zip_homeo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45714">
        <property type="entry name" value="HOMEOBOX-LEUCINE ZIPPER PROTEIN HAT14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45714:SF11">
        <property type="entry name" value="HOMEOBOX-LEUCINE ZIPPER PROTEIN HAT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02183">
        <property type="entry name" value="HALZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04618">
        <property type="entry name" value="HD-ZIP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00340">
        <property type="entry name" value="HALZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 8 9" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7 8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="108"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="8">
        <location>
            <begin position="110"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="60"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="231"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="174"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="68"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="86"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="231"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="260"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="276"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9680596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="285" mass="31927" checksum="19921BD7727E1096" modified="2024-01-24" version="1">MVDKDDDLGLSLALKCPEALPQRPLNFFVQKKAHCNDGFHASETRAYLRGIDVNRAPTMTDCEDVGVSSPNSTISSISGKRNDRETNEEENENENEIESSEEDAGGTGDTVRKKLRLSKEQSAILEETFKEHNTLNPKQKSALAKQLNLRPRQVEVWFQNRRARTKLKQTEVDCEYLKKCCENLTEENRRLQKEVQELRTLKLSPQLYMHMNPPTTLTMCPSCERVAVTSASSSAPSPSHASNPLAAAPHFPLGPHQHRPMPMAGPWAGAASMRHRPFDAHGTRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z1Z3</accession>
    <name>A0AA38Z1Z3_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_020032</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z1Z3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9680901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="159" mass="15316" checksum="C763D771C8F5BABA" modified="2024-01-24" version="1">MTVASSFNSAGAVTSTADLSDVLFSDSAGESDGEPSGDSSGGAGGEETGVGASAAGEGLGETAGVGEGDDTGSGAGAGDGDFPNNSVGRSKLSTVKRQSGVASSTVSATLDESTPVFRVTASPAVDTVRSYFPAPLVASVLIGSLLVFNDPIGVYEATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z3L9</accession>
    <name>A0AA38Z3L9_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_017840</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the peptidase A1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9681635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z3L9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06098">
        <property type="entry name" value="phytepsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000044">
        <property type="entry name" value="Lysosomal aspartic protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000115">
        <property type="entry name" value="Lysosomal aspartic protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.225.10">
        <property type="entry name" value="Saposin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033869">
        <property type="entry name" value="Phytepsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007856">
        <property type="entry name" value="SapB_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008138">
        <property type="entry name" value="SapB_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011001">
        <property type="entry name" value="Saposin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008139">
        <property type="entry name" value="SaposinB_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF28">
        <property type="entry name" value="OS01G0290000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05184">
        <property type="entry name" value="SapB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03489">
        <property type="entry name" value="SapB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47862">
        <property type="entry name" value="Saposin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50015">
        <property type="entry name" value="SAP_B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="6 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 10" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041443080" evidence="11">
        <location>
            <begin position="27"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="13">
        <location>
            <begin position="83"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="domain" description="Saposin B-type" evidence="12">
        <location>
            <begin position="313"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="Saposin B-type" evidence="12">
        <location>
            <begin position="372"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="101"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="288"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="114"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9681635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="502" mass="54747" checksum="EC69BF1B8B4AD171" modified="2024-01-24" version="1">MRQGVVWAAFCLWALICPLLPVYSHGSVRIGLKKRPLDFNNMRTARIAQMQGKIGGGVLSKYHGFDDPDGEYVSLKNYLDAQYFGEIGIGTPPQNFTVVFDTGSSNLWVPSSKCYFSIACFFHNKYKARLSSTYTKIGRPGEIHYGSGSISGFFSQDNVEVGSLVVKDQVFIEATREGSLTFALAKFDGIMGLGFQGISVGNATPVWSTMLQQGLLHEELFSFWLNRNPNANEGGEIVFGGVDKRHFRGKHTFVPVTQAGYWQFRMGDFLISNQTTGVCEGGCSAIVDSGTSLIAGPTLVVTQINHAIGAEGIVSMECKEVVSQYGNMMWDLLVSGVLPSKVCSQIGLCMASPAIRTVVEKEKMEPVEEVGDVVFCNACEMIAVWIQSQLKQMKTKDKVLRYVTELCGSLPSPMGESVIDCTSVANMPNITFIIGDKAFDLTPDQYILRTGDGSATVCLSGFTALDVPPPKGPLWILGEIFMGVYHTVFDFGDLRIGFAEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z5B9</accession>
    <name>A0AA38Z5B9_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_018308</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z5B9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45613:SF207">
        <property type="entry name" value="OS08G0300700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45613">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12854">
        <property type="entry name" value="PPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13812">
        <property type="entry name" value="PPR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="102"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="137"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="172"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="207"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="242"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="277"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="312"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="347"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="382"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="417"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="452"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="487"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="522"/>
            <end position="556"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9682362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="586" mass="65293" checksum="8D38DD5B3B488F59" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2025-10-08" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z5N7</accession>
    <name>A0AA38Z5N7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_018446</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057135">
        <property type="entry name" value="At4g27190-like_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050905">
        <property type="entry name" value="Plant_NBS-LRR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463:SF198">
        <property type="entry name" value="RPP4C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23247">
        <property type="entry name" value="LRR_RPS2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0611">Plant defense</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="174"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9682527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1611" mass="185026" checksum="6F22A04D6745C544" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z681</accession>
    <name>A0AA38Z681_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Metalloenzyme domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_018820</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglycerate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R)-2-phosphoglycerate = (2R)-3-phosphoglycerate</text>
            <dbReference type="Rhea" id="RHEA:15901"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="EC" id="5.4.2.12"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the BPG-independent phosphoglycerate mutase family. A-PGAM subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z681"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004619">
        <property type="term" value="F:phosphoglycerate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006124">
        <property type="entry name" value="Metalloenzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004456">
        <property type="entry name" value="Pglycerate_mutase_ApgM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31209">
        <property type="entry name" value="COFACTOR-INDEPENDENT PHOSPHOGLYCERATE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31209:SF0">
        <property type="entry name" value="METALLOENZYME DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01676">
        <property type="entry name" value="Metalloenzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metalloenzyme" evidence="6">
        <location>
            <begin position="66"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9682982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="210" mass="22578" checksum="E0F431677092E444" modified="2024-01-24" version="1">MHLTESKVAVGCRTLLTSKATAIAKALSAPLQSCPSVFVPGEDEHKPAEQMATILGFYTLRLLMIVGHDKASIFKVKAMEAVDRAIGQLASLFWQAESTRNFEYHLCVTGDHSTPVDYGDHALEPVPFATDFVGATGVDAVIMKTSLEPFPLPTIKCGEDQTEDLEMMEERSKQAFSGDSVSGFGEVAAARGCLGRFSGSEMIGIVQTCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z6C0</accession>
    <name>A0AA38Z6C0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Nodulin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_018997</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z6C0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd17354">
        <property type="entry name" value="MFS_Mch1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000446">
        <property type="entry name" value="Nodulin family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056555">
        <property type="entry name" value="NFD4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010658">
        <property type="entry name" value="Nodulin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21576:SF73">
        <property type="entry name" value="F1C9.29 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21576">
        <property type="entry name" value="UNCHARACTERIZED NODULIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23262">
        <property type="entry name" value="NFD4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06813">
        <property type="entry name" value="Nodulin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="174"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="210"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="238"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="357"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="398"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="430"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="457"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="489"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="553"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="domain" description="Nodulin-like" evidence="6">
        <location>
            <begin position="15"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="NFD4 C-terminal" evidence="7">
        <location>
            <begin position="357"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF06813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9683235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="591" mass="64846" checksum="85929DCF735290A5" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z6F6</accession>
    <name>A0AA38Z6F6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_018915</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z6F6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF78">
        <property type="entry name" value="OS12G0587133 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="321"/>
            <end position="601"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9683117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1053" mass="121565" checksum="6D53FB7CA1C1B000" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z7Y1</accession>
    <name>A0AA38Z7Y1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_016528</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z7Y1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19179">
        <property type="entry name" value="SET_RBCMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1420.10:FF:000012">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1410.10:FF:000005">
        <property type="entry name" value="Ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit N-methyltransferase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1420.10">
        <property type="entry name" value="Rubisco LSMT, substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1410.10">
        <property type="entry name" value="set domain protein methyltransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015353">
        <property type="entry name" value="Rubisco_LSMT_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036464">
        <property type="entry name" value="Rubisco_LSMT_subst-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044431">
        <property type="entry name" value="SET_RBCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050600">
        <property type="entry name" value="SETD3_SETD6_MTase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271:SF134">
        <property type="entry name" value="OS01G0976450 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271">
        <property type="entry name" value="UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09273">
        <property type="entry name" value="Rubis-subs-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81822">
        <property type="entry name" value="RuBisCo LSMT C-terminal, substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SET" evidence="4">
        <location>
            <begin position="53"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9684078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="473" mass="53255" checksum="421E592097053938" modified="2024-01-24" version="1">MLLGARFTDRCCLHRPIVCLSRRFKFSSPAKPQDSHCVDKDCDDFLPWLEQKAGVEISSVLSIGKSTYGRSLFASKSIQTGDCILKVPYNVQISPDNVPSKINYLLGDEVGNIAKLAIVILVEWKIGQDSEWAPYINRLPQPGEMHSTIFWSEGELKMIQQSSVYQETVNQKAQIQKDFLAIKPVLHHFSENLFKDISLKEFMHACALVGSRAWGSTKGLSLIPFADFVNHDGFSESVLLSDEDKQLSEVIADRNYAPGEQVLIRYGKFPNATLLLDFGFTLPYNIYDQVQIQVNIPHHDLLRTLKLELLHRHCPPKIGDVNSFSSMGNSFTIKEVKSARGKGRGIPQSLRAFARIFCCTSPQELSDLAVEAAQNDGRLARRPLKSWNREIQAHQVLLSWITQLINEYNTSIKSLPPASPLYETFALRKQMARDLLTGELRVLKSAGAWLENYCATLTTKSYLSDQNGSRAKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z8J6</accession>
    <name>A0AA38Z8J6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">rRNA methyltransferase 2, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_016665</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z8J6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008650">
        <property type="term" value="F:rRNA (uridine-2'-O-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000212">
        <property type="entry name" value="Ribosomal RNA large subunit methyltransferase E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01547">
        <property type="entry name" value="RNA_methyltr_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050082">
        <property type="entry name" value="RNA_methyltr_RlmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002877">
        <property type="entry name" value="RNA_MeTrfase_FtsJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015507">
        <property type="entry name" value="rRNA-MeTfrase_E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920">
        <property type="entry name" value="RIBOSOMAL RNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920:SF18">
        <property type="entry name" value="RRNA METHYLTRANSFERASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01728">
        <property type="entry name" value="FtsJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005461">
        <property type="entry name" value="23S_rRNA_mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribosomal RNA methyltransferase FtsJ" evidence="9">
        <location>
            <begin position="19"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="155"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="159"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00041184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR005461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9684289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="233" mass="25125" checksum="65B7696CEE5C1B4D" modified="2024-01-24" version="1">MSGAGTPDFFYREAQRLGYAARSAFKLLQMQKQYKLIKPGSSVLDLGCAPGAWLQVACQCLGPLKNGGVVVGIDLKKVKVPSMHCDSRVKTVCADVMKLPKDEIRAFSPQRKGFSVILSDMCPLVSGITTKDAVLSAELGMQALHLAVGGPALAPSDDEIQKGEHHDDSTSGPDDDGILQPGGHLVIKLLESEDAQGFSQICKPLFRKASWLRPKATRSSSREIYLICQGLRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z9K4</accession>
    <name>A0AA38Z9K4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_016999</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z9K4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005162">
        <property type="entry name" value="Retrotrans_gag_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33223">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33223:SF8">
        <property type="entry name" value="OS04G0172440 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03732">
        <property type="entry name" value="Retrotrans_gag"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Retrotransposon gag" evidence="1">
        <location>
            <begin position="73"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9684797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="127" mass="14922" checksum="916A361A31855D7D" modified="2024-01-24" version="1">MDRLEQRLRQLRTPDRAVTWEDFDEAPVASLPPDFKMPDFEKFTGIGCPRIHLRLYSILMRAHGLEEPHLITLFPLSLSGAAQRWFASSESSRCRTWDDLAQEFLRQFSFNTVVDVSRRELEALRQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z9R2</accession>
    <name>A0AA38Z9R2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Nucleolar GTP-binding protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_017178</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in the biogenesis of the 60S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
            <location evidence="1 6">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family. NOG subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z9R2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01897">
        <property type="entry name" value="NOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000496">
        <property type="entry name" value="Nucleolar GTP-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024926">
        <property type="entry name" value="NOG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041623">
        <property type="entry name" value="NOG1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010674">
        <property type="entry name" value="NOG1_Rossman_fold_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012973">
        <property type="entry name" value="NOG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45759">
        <property type="entry name" value="NUCLEOLAR GTP-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06858">
        <property type="entry name" value="NOG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17835">
        <property type="entry name" value="NOG1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08155">
        <property type="entry name" value="NOGCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038919">
        <property type="entry name" value="NOG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="OBG-type G" evidence="8">
        <location>
            <begin position="154"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="461"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="500"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="619"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="515"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="546"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="565"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="579"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="619"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="644"/>
            <end position="661"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR038919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9685038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="661" mass="75441" checksum="7FA1828AC6252D0B" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZA14</accession>
    <name>A0AA38ZA14_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_017177</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZA14"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054722">
        <property type="entry name" value="PolX-like_BBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35317:SF35">
        <property type="entry name" value="DUF4219 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35317">
        <property type="entry name" value="OS04G0629600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22936">
        <property type="entry name" value="Pol_BBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="4">
        <location>
            <begin position="297"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="321"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="401"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="534"/>
            <end position="565"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9685037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="846" mass="96529" checksum="E8683A8161FDC7D9" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZBX3</accession>
    <name>A0AA38ZBX3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glutathione transferase</fullName>
            <ecNumber evidence="3">2.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_015058</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GST superfamily. Phi family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9685985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZBX3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043295">
        <property type="term" value="F:glutathione binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009407">
        <property type="term" value="P:toxin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03187">
        <property type="entry name" value="GST_C_Phi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03053">
        <property type="entry name" value="GST_N_Phi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000004">
        <property type="entry name" value="Glutathione S-transferase F2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000016">
        <property type="entry name" value="Glutathione S-transferase F2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034347">
        <property type="entry name" value="GST_Phi_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43900">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43900:SF65">
        <property type="entry name" value="GLUTATHIONE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0216">Detoxification</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="8">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="9">
        <location>
            <begin position="88"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9685985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="209" mass="24044" checksum="DDE6068C20E3B6EA" modified="2024-01-24" version="1">MVVKVYGSAFACAKRVLICLVEKEIEFETVPVDIIKGEHKDPEYLKLQPFGVVPVIQDGDYTLFESRAIIRYYAEKYKSQGTDLLGKTIEERGVVEQWLEVEAQNYHPPIYNLALHVLFSDKMGFPADAKVIEESEKNLGKVLDIYEERLSKSKYLAGDFFSLADLSHIPFANYLGVMGKMYLIEERKHVSAWWDDISSRPSWKKVLEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZC68</accession>
    <name>A0AA38ZC68_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_017764</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZC68"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000022">
        <property type="entry name" value="Beta-glucosidase 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001360">
        <property type="entry name" value="Glyco_hydro_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018120">
        <property type="entry name" value="Glyco_hydro_1_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353">
        <property type="entry name" value="GLYCOSYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353:SF297">
        <property type="entry name" value="VICIANIN HYDROLASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00232">
        <property type="entry name" value="Glyco_hydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00131">
        <property type="entry name" value="GLHYDRLASE1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00572">
        <property type="entry name" value="GLYCOSYL_HYDROL_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041258185" evidence="5">
        <location>
            <begin position="24"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9685828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="507" mass="57262" checksum="286BD07EA2F5A0C6" modified="2024-01-24" version="1">MATQGPLLFCALVLVLSFAHCQGVKPSAMFSRHSFPPGFTCGAASSAYQYEGAAHLRGKNIWDTFTAKHPEKISDHSTGDVANDFYHKYKEDIQLLKFLGMDALRFSISWTRVLPTGRVSGGVSKDGVQFYNNVINELLANGLKPFVTLFHWDLPQALEDEYGGFLSPKIVDDYRNYVDFCFKQFGDRVKHWITLNEPFSYSYYGYSTGTFAPGRCSNYSGTCASGNSATEPYKVAHHLLLSHAAGVKLYKEKYQKSQKGIIGVTLVTHWLQSKYATVAGVKASRRALDFMLGWFLHPITYGEYPMTMQSLVGHRLPKFSPAESEMLKGSLDFLGINYYTSNYATTYASTVNTLELSWAIDGRLNLTTEKDGVNIGQPTPLNWLYICPWGIRKLMLYIKERYNNPTIYITENGLATANNASVPVKEDLNDTLRITYHRGHLYYLSKAIKEGVNVKGYFAWSFLDDFEWDAGFTFRFGLGYVDYKNGLKRYPKHSLIGSRSSSKNKKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZDN9</accession>
    <name>A0AA38ZDN9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Ubiquinol oxidase</fullName>
            <ecNumber evidence="18">1.10.3.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PVL29_015727</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 18">
            <text>2 a ubiquinol + O2 = 2 a ubiquinone + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:30255"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="EC" id="1.10.3.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17 18">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="17 18">Binds 2 iron ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer; disulfide-linked.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the alternative oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.10.3.11" evidence="18"/>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZDN9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098803">
        <property type="term" value="C:respiratory chain complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009916">
        <property type="term" value="F:alternative oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106292">
        <property type="term" value="F:superoxide-generating NADPH oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102721">
        <property type="term" value="F:ubiquinol:oxygen oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010230">
        <property type="term" value="P:alternative respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01053">
        <property type="entry name" value="AOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1260.140:FF:000001">
        <property type="entry name" value="Ubiquinol oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.140">
        <property type="entry name" value="Alternative oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002680">
        <property type="entry name" value="AOX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038659">
        <property type="entry name" value="AOX_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31803">
        <property type="entry name" value="ALTERNATIVE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31803:SF6">
        <property type="entry name" value="UBIQUINOL OXIDASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01786">
        <property type="entry name" value="AOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005229">
        <property type="entry name" value="AOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 18" id="KW-0249">Electron transport</keyword>
    <keyword evidence="14 17 18" id="KW-0408">Iron</keyword>
    <keyword evidence="16 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 17 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13 18" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 18" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="7 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="binding site" evidence="17">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="293"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR005229-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU003779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ9686987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="320" mass="36723" checksum="E57A884D03B4D070" modified="2024-01-24" version="1">MNQLVARSLMRGLINGAKCNRSILCSVTRVELRDEKAAFGRMMSTETALAEKNQKEKSVEEVEKQSEVVFSSYWGIQRPKITREDGSPWPWNCFMPWETYQADTSIDLSKHHVPKTFVDKVAYRTVKLLRIPTDIFFQRRYGCRAMMLETVAAVPGMVGGMLLHLRSLRKFEHSGGWVKALLEEAENERMHLMTMVELVRPKWYERLLVLTVQGVFFNAFFVLYVLSPKAAHRVVGYLEEEAIHSYTEFLKDIDSGAIENVPAPAIAIDYWRLPKDATLKDVITVIRADEAHHRDVNHFASDIHFQGKKLNEAPAPIGYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZES1</accession>
    <name>A0AA38ZES1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Nuclear proteasome inhibitor UBLCP1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_016147</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 17">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZES1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090364">
        <property type="term" value="P:regulation of proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01813">
        <property type="entry name" value="Ubl_UBLCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011943">
        <property type="entry name" value="HAD-SF_hydro_IIID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051658">
        <property type="entry name" value="UBLCP1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02245">
        <property type="entry name" value="HAD_IIID1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48493">
        <property type="entry name" value="UBIQUITIN-LIKE DOMAIN-CONTAINING CTD PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48493:SF1">
        <property type="entry name" value="UBIQUITIN-LIKE DOMAIN-CONTAINING CTD PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="12">
        <location>
            <begin position="11"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="FCP1 homology" evidence="13">
        <location>
            <begin position="138"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9687542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="327" mass="37878" checksum="C81D80526E7CB90C" modified="2024-01-24" version="1">MASSSLSEEELTLTVKWSGNEYTVRVCGDDSVGELKRRICQLTNVLPKRQKLLYPKLGSKLADDSVLLSQLPLKPSLKMTMIGTVEDDIIVDQVDSPEIIDDFELGQDESTDIKDKEVNKQKLRRRIYQYKIELRNPCREGKKLLVLDIDYTLFDHRSTAENPLELMRPYLHEFLSAVYAEYDIIIWSATSMKWVELKMGQLGVLNNPNYKITALLDHLAMITVHSDSRGPFDCKPLGLIWGQFPEFYSSKNTIMFDDLRRNFVMNPQNGLTIKPFRKAHANRDSDQELVKLTQYLLAIAELDDLSSLNHKNWESYNEDNFKRRRHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZG56</accession>
    <name>A0AA38ZG56_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Core-2/I-branching beta-1,6-N-acetylglucosaminyltransferase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_016263</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZG56"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044174">
        <property type="entry name" value="BC10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003406">
        <property type="entry name" value="Glyco_trans_14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31042">
        <property type="entry name" value="CORE-2/I-BRANCHING BETA-1,6-N-ACETYLGLUCOSAMINYLTRANSFERASE FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31042:SF3">
        <property type="entry name" value="OS08G0110400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02485">
        <property type="entry name" value="Branch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9687704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="379" mass="43768" checksum="C6C3656055781E45" modified="2024-01-24" version="1">MKSTTVDRHIGLLNLAKTLSFLVVFVAGVVVGMTASSQINRYFVSQAKLYFYSNMNNHFSAMQIPNPTLRNCSSEANLTHGFSDNELFWRASMVPRRAKYPFKRVPKVAFMFLTRGPLPLMPLWERFFEGHRKYFSVYVHALPGFELNVSMHSAFYKRQIPSKNVEWGTVALAEAERRLLANALLDFSNERFVLLSESCIPIYNFRTVYKYLINSDYSFVESYDDPSRYGRGRYSRNMLPDIKLYQWRKGSQWFELSRELAVNIVADTKYYTLFKKYCKPSCYPDEHYIQTFLNMFYGSLNANRSVTWVDWSMGGPHPATLGAANITEGFIQAIRNNGTVCPYNSEKTSVCYLFARKFAPSALEPLLNLTSKVMGFKNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZI96</accession>
    <name>A0AA38ZI96_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">adenylate kinase</fullName>
            <ecNumber evidence="5">2.7.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">ATP:AMP phosphotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_014494</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>AMP + ATP = 2 ADP</text>
            <dbReference type="Rhea" id="RHEA:12973"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.3"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the adenylate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.3" evidence="5"/>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZI96"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:AMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01428">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001694">
        <property type="entry name" value="Adenylate kinase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00235">
        <property type="entry name" value="Adenylate_kinase_Adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006259">
        <property type="entry name" value="Adenyl_kin_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000850">
        <property type="entry name" value="Adenylat/UMP-CMP_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033690">
        <property type="entry name" value="Adenylat_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018962">
        <property type="entry name" value="DUF1995"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01351">
        <property type="entry name" value="adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23359">
        <property type="entry name" value="NUCLEOTIDE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00406">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09353">
        <property type="entry name" value="DUF1995"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00094">
        <property type="entry name" value="ADENYLTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00113">
        <property type="entry name" value="ADENYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0934">Plastid</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="467"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1995" evidence="16">
        <location>
            <begin position="330"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="275"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF09353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9688873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="492" mass="55048" checksum="CD1DE2AC64F5FED3" modified="2024-01-24" version="1">MLQSSLAPPSHLHCTNLYSVPSLPLLCSLPLSFSSSSLSFGLRRCRRLSFGVHVDTHFSINPEPKGLRVSCAISEPLKVMISGAPASGKGTQCEMIVQKFGLVHISTGDLLRAEVSSGSEIGNKAKEYMDTGRLVPDEIVIMMVTARISQEDTKEKGWLLDGFPRTFAQAQSLEKLKIRPDVYIVLDVPDQILIDRCVGRRVDPVTGKIYHLKFFPPENEEIKARLVTRSDDTEEKVKSRLEIYKKNAEAILSTYSTIMNKVDGNHSKDEVFKAIDSLLSKVQKDKENMMKLEKSIRASERLSNLVSSSEENWRGVPTRLNNIPHSREIRTYFYDDVLQATQRAVNDGRTRLKVEINIPELNPEMDVYRIGTLMELVRVLALSFADDGKHVKVCVQGSMGEGALAGMPLQLAGTRKILEFMDWGDYGAKGTFINIGSIGMHLKTKNICICMTSSCLSPPYAAVSHHIHHVIILSVFQILHQWFIISLFLAFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZJQ4</accession>
    <name>A0AA38ZJQ4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">PVL29_014952</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="25">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="25">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9689519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZJQ4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000101">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000358">
        <property type="entry name" value="Putative leucine-rich repeat receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000661">
        <property type="entry name" value="Serine-threonine protein kinase plant-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051809">
        <property type="entry name" value="Plant_receptor-like_S/T_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27008">
        <property type="entry name" value="OS04G0122200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27008:SF497">
        <property type="entry name" value="OS11G0695000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 22" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="13 22" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="11 23" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="23">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041397189" description="non-specific serine/threonine protein kinase" evidence="23">
        <location>
            <begin position="23"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="24">
        <location>
            <begin position="647"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="675"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="KAJ9689519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="879" mass="97234" checksum="08E718A5BF32C67D" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZM66</accession>
    <name>A0AA38ZM66_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Fe(2+) transport protein 1-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_013648</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ZIP transporter (TC 2.A.5) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZM66"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004698">
        <property type="entry name" value="Zn/Fe_permease_fun/pln"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00820">
        <property type="entry name" value="zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040:SF41">
        <property type="entry name" value="ZINC TRANSPORTER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040">
        <property type="entry name" value="ZINC/IRON TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041351667" description="Fe(2+) transport protein 1-like" evidence="9">
        <location>
            <begin position="26"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="49"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="127"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="227"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="259"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="287"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="329"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9691532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="349" mass="37548" checksum="9CD572B83D838928" modified="2024-01-24" version="1">MASLKNLGALTLLLLLLSLTVQTWGKEDADGAGSECRVAKEVSEEKASALKLKVIAIFTILTASILGISSPILVRGMPLFKQDGKVFVLVKAFASGVILATGYVHVLPDSFECLTSPCLPDYIWSKFPFTTFIAMVAAVLTLMMDSFAMSYYRKHGMNEVECDHGNLIEHGQGHSHGVGVKKLDEEASKLLRYQIIAQLLELGIVVYSVVIGLSMGASQNARTIRPLIAALCFHQLFEGMGLGACLLQAEYNARMKAIMVFFFSVTTPLGIALGIGLSHVYSDNSPASLIVVGVLNATSAGLLNYMALVDLLAADFMGTKLQSNMKLQMWAFIAVLLGVGAMSLMAKWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZPF6</accession>
    <name>A0AA38ZPF6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">1-phosphatidylinositol 4-kinase</fullName>
            <ecNumber evidence="2">2.7.1.67</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_011794</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PI3/PI4-kinase family. Type II PI4K subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.67" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZPF6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004430">
        <property type="term" value="F:1-phosphatidylinositol 4-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.110">
        <property type="entry name" value="Met-10+ protein-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12030">
        <property type="entry name" value="Uncharacterised protein family UPF0261, NC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044571">
        <property type="entry name" value="P4KG1-8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056744">
        <property type="entry name" value="TRM5/TYW2-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056778">
        <property type="entry name" value="UPF0261_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45800:SF11">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3-KINASE-RELATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45800">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-KINASE GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25133">
        <property type="entry name" value="TYW2_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23189">
        <property type="entry name" value="UPF0261_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="UPF0261" evidence="7">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="TRM5/TYW2-like N-terminal" evidence="8">
        <location>
            <begin position="168"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF25133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9692868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="251" mass="28044" checksum="89BAAC32CB751A2C" modified="2024-01-24" version="1">MVNFEAKITIPSHLLKRNIHVHNEQVSLVQTTVDENKRFASFIANKLNKASSEVCVYLPQKGIPALDAPGKTLGQPGLKHSVRVRGTGFKEVAAYLLDYNHFANVPSTALVKITHSIFNANDRVSKIASLQQFIPHDFDAGDHSTSSFTVSAVHRIGTLEIWISKHRNLSSQLLEQLPTKWKCLEDIVVLPVTSFKDQYKTQLEMNFSLSLLNHSNACRLARQGRVVPSRTRDSTFEILMGAAKLKSAKEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZR72</accession>
    <name>A0AA38ZR72_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Retrotransposon protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_012304</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9693469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZR72"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46250">
        <property type="entry name" value="MYB/SANT-LIKE DNA-BINDING DOMAIN PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46250:SF15">
        <property type="entry name" value="OS01G0523800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="109"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9693469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="221" mass="25314" checksum="F9F86C48C4476F08" modified="2024-01-24" version="1">MDMLTHGSRFSWDNAKKMVLCDQDVFEGWVKVNILTILPILYFYSTTSWHKDAYGLRLKPFLHFDDLSLVFYKDRANGKGAMSAADILEELDQGEASNDIGVDDLEAEADASRSNTVASTPSRMERSSQSRRKIAKNDDTTLINVMTRTCNALESLVGNFNQQTERENRVIVELEKLHNLNRLDILKLSQIIMNDPMKVKLLFNLDEDLKVEWVKQLLQTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZSN9</accession>
    <name>A0AA38ZSN9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Interactor of constitutive active ROPs 2, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_010149</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ICR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZSN9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029688">
        <property type="entry name" value="ICR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34224:SF4">
        <property type="entry name" value="INTERACTOR OF CONSTITUTIVE ACTIVE ROPS 2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34224">
        <property type="entry name" value="INTERACTOR OF CONSTITUTIVE ACTIVE ROPS 2, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="300"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="589"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="368"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="521"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="64"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="99"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9694516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="622" mass="69602" checksum="059DD875B85A79E3" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZUD8</accession>
    <name>A0AA38ZUD8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF4005 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_010785</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Binds to multiple calmodulin (CaM) in the presence of Ca(2+) and CaM-like proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IQD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZUD8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025064">
        <property type="entry name" value="DUF4005"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32295:SF212">
        <property type="entry name" value="CALMODULIN BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32295">
        <property type="entry name" value="IQ-DOMAIN 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13178">
        <property type="entry name" value="DUF4005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4005" evidence="4">
        <location>
            <begin position="346"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9695478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="425" mass="48046" checksum="92B0EF6D7E33A165" modified="2024-01-24" version="1">MGKKRSWFSLVKRIFTSEAKEKEEKKPKSWRCLFTRFKLKQCPTLAAPRRTLTEARAEQRKHALTVALATAAAAEAAVAAARAAAEVVRLTCSPPSCYHTCDKRNQDLAAIKIQTAFRGYLARKALQALKGLVRLQALVRGQIVRRQAITNLKCLPSTANTRAQVNIGGVLTTEETYKDGNNRKFLRPKKECGGREIKAYVIEQPEGSGQRSWDYNILSQEDVETIWLRKQEAAIRRQRMKKYSSSHRERINAQMPEETVPYKENGRQSCQLVRWMDRMEHKRKEVENSKAAADSNPLNGHINGTTNIELRNEWKQDSTEGSEMPFSLPRRSFCHRKHNSVIDDSSIPSSPVFPTYMATTESAKAKARALSTPKQRLGFLDSCFDHSSPCTNRPSFWSSLNGESISSSGRSGYSWQNTVSMKSLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZUQ7</accession>
    <name>A0AA38ZUQ7_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_010711</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZUQ7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080043">
        <property type="term" value="F:quercetin 3-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080044">
        <property type="term" value="F:quercetin 7-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000040">
        <property type="entry name" value="UDP-glycosyltransferase 76C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000120">
        <property type="entry name" value="UDP-glycosyltransferase 76C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926">
        <property type="entry name" value="GLUCOSYL/GLUCURONOSYL TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926:SF1374">
        <property type="entry name" value="UDP-GLYCOSYLTRANSFERASE 76F1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9695374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="458" mass="50993" checksum="4E863229252862F4" modified="2024-01-24" version="1">MPLQDMEKRRQGIRDHLVLVPCPFQGHMKPMLHLANLLHSKGFSITIIHSQSNSPNPSHYPHFFFRSLGDSSHIQSASDGDFVPFISALNLHSPTIFRDLLLRMHFQDPVLSIIHDSAMYFPVTVADELDIPRIVLRTSSAAAGFAFALSILKQQRSFPFQENESEEALVEFPSIRGKDMPVINTFHKEARDEFLARVHHGTKTASAIVWNTFRGLEQTTLEKIEQLFSVPNFPLGPLHKHSGASLTSFATEDHSCIAWLDQQAPSSVIYVSIGSLITTSESELVEMAWGLANSGQPFLWVVRPGLVNGSSNAAQLLPKEFKETTNKRGRVISWAPQEAVLAHRSVGGFWTHSGWNSTVESISEGVPMLCSPIVGDQRVNARFVSHVWRTGIQLEDGVERGKIEKAIKRLMVDEEGAEMKKRAMDLKDKVASSLRQGGSSSEFLHSLVDFIKGKLCNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZVC5</accession>
    <name>A0AA38ZVC5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_010881</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZVC5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000042">
        <property type="entry name" value="probable strigolactone esterase DAD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43039">
        <property type="entry name" value="ESTERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="39"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="4">
        <location>
            <begin position="26"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9695615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="270" mass="29880" checksum="13E092FBC2ACC2D0" modified="2024-01-24" version="1">MDMLSESGGGIIEALNATVHGNGTRTLVLSHGFGLDQSVWHYLIPYLACYFKVVVFDLIFVNPNLYDPKKNSNFDSYAHDLVCLLDQLNVKKTIYLGHSMSAMIGCIAATKRPDLFEHLILLGGSPRYLNAEGYYGGFERSDIDKIFEAINKNFPVWVQNFAPMAVGINNSAAIAEFEYSLGRMKPEIALSVAKTVFLSDLRLILPQVKVPCTIIQSREDIVAPTFIACYMKENLGGDATVKILETQGHFPQLTAFPLLLDALNQVLSIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZVN2</accession>
    <name>A0AA38ZVN2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF4283 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_010589</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZVN2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34427">
        <property type="entry name" value="DUF4283 DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34427:SF5">
        <property type="entry name" value="DUF4283 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="351"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="531"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="621"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="374"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9695174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="822" mass="90578" checksum="810E07FD6016636E" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZYY6</accession>
    <name>A0AA38ZYY6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Aminotransferase class V domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_008844</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZYY6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43092">
        <property type="entry name" value="L-CYSTEINE DESULFHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43092:SF7">
        <property type="entry name" value="L-CYSTEINE DESULFHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="2">
        <location>
            <begin position="82"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9696839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="451" mass="50265" checksum="F5A6283B8B3CF659" modified="2024-01-24" version="1">MDSDRGENGDSSHNHVSKKPKLSVFISEEEIRQEFSHHQRGIARINNGSFGSCPASIIAAQKEWQLRFLQQPDDFYFNHLRKGLLESRTVVKGLINADSVDEVSLIDNATTAAAIVLQQIGRAFAQGKFQKGDVVVMLHCAFQSVKKSIQAYVTGAGGSVIEVQLPFPVTSNEEIVSEFRKGLEKGKSDGRHVRLAIIDHITSMPCVVVPVEELVKICRQEGVDQVFVDAAHAIGSVPVDVKEIGADFYVSNLHKWFFCPPSVAFLYCRKSPLSSEVHHPVVSHEFGNGLAIESSWIGTRDYSSQLVVPSVLEFVNRFEGGIEGIMMRNHEIVVKMGEMLAKSWGTNLGAPPEMCASMIMVGLPSRLFISSEEDAMRLRSYLRQHHGIEVPLHYQAPSDVEGVPKDKDGLITGYARISHQVYNSFDDYCKFRDAINQLVEQRRSCKMLFTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A0E3</accession>
    <name>A0AA39A0E3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF3527 domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_007574</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A0E3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021916">
        <property type="entry name" value="DUF3527"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31390:SF4">
        <property type="entry name" value="DUF3527 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31390">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12043">
        <property type="entry name" value="DUF3527"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="297"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="395"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="509"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="134"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="415"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="439"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="524"/>
            <end position="547"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9698566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="952" mass="104781" checksum="7DDD0C3BD4AF67F3" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A171</accession>
    <name>A0AA39A171_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Alkaline/neutral invertase</fullName>
            <ecNumber evidence="6">3.2.1.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_007854</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Invertase that cleaves sucrose into glucose and fructose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.</text>
            <dbReference type="EC" id="3.2.1.26"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the glycosyl hydrolase 100 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.26" evidence="6"/>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A171"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033926">
        <property type="term" value="F:endo-alpha-N-acetylgalactosaminidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004575">
        <property type="term" value="F:sucrose alpha-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005987">
        <property type="term" value="P:sucrose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000001">
        <property type="entry name" value="probable alkaline/neutral invertase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024746">
        <property type="entry name" value="Glyco_hydro_100"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31916">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31916:SF15">
        <property type="entry name" value="ALKALINE_NEUTRAL INVERTASE D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12899">
        <property type="entry name" value="Glyco_hydro_100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5 6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9698986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="519" mass="58939" checksum="DBBD9448CCBBB0A7" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A488</accession>
    <name>A0AA39A488_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">F-box/LRR-repeat protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_005822</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A488"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22159">
        <property type="entry name" value="F-box_AtTIR1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000023">
        <property type="entry name" value="F-box/LRR-repeat protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001196">
        <property type="entry name" value="F-box/LRR-repeat protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057207">
        <property type="entry name" value="FBXL15_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318:SF41">
        <property type="entry name" value="F-BOX_LRR-REPEAT PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318">
        <property type="entry name" value="PARTNER OF PAIRED, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25372">
        <property type="entry name" value="DUF7885"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="10"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="F-box/LRR-repeat protein 15-like leucin rich repeat" evidence="2">
        <location>
            <begin position="136"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="F-box/LRR-repeat protein 15-like leucin rich repeat" evidence="2">
        <location>
            <begin position="314"/>
            <end position="506"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9700182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="535" mass="58263" checksum="F1892391FF20FFA1" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A4E4</accession>
    <name>A0AA39A4E4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">PGG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_006046</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A4E4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026961">
        <property type="entry name" value="PGG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24186:SF50">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN ITN1-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24186">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13962">
        <property type="entry name" value="PGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="454"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="495"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="539"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="564"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="96"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="252"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="286"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="PGG" evidence="9">
        <location>
            <begin position="445"/>
            <end position="556"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9700549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="593" mass="65358" checksum="55A944C6977E948C" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A5K5</accession>
    <name>A0AA39A5K5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_006590</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A5K5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005504">
        <property type="term" value="F:fatty acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009627">
        <property type="term" value="P:systemic acquired resistance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.110.10">
        <property type="entry name" value="Plant lipid-transfer and hydrophobic proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036312">
        <property type="entry name" value="Bifun_inhib/LTP/seed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016140">
        <property type="entry name" value="Bifunc_inhib/LTP/seed_store"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039265">
        <property type="entry name" value="DIR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33122:SF63">
        <property type="entry name" value="BIFUNCTIONAL INHIBITOR_PLANT LIPID TRANSFER PROTEIN_SEED STORAGE HELICAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33122">
        <property type="entry name" value="LIPID BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14368">
        <property type="entry name" value="LTP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47699">
        <property type="entry name" value="Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9701312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="114" mass="11894" checksum="AE4F25CBD787D131" modified="2024-01-24" version="1">MSLGIIPNVRQTPYSYNKAFGKLAIVALVLAALASSNAQTICNMSGEGLMACKPSVAPPNPAPPSAACCSALSHADIHCLCSYRNSKLLPSMGIDPNLALQLPKKCNFPNPAHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A696</accession>
    <name>A0AA39A696_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_006926</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A696"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080043">
        <property type="term" value="F:quercetin 3-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080044">
        <property type="term" value="F:quercetin 7-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000019">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926">
        <property type="entry name" value="GLUCOSYL/GLUCURONOSYL TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926:SF1560">
        <property type="entry name" value="UDP-GLYCOSYLTRANSFERASE 74E1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9701763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="456" mass="51103" checksum="A220D69C07958208" modified="2024-01-24" version="1">MERYTRITEPHVMVFPFPLQGHISPMFQFCKRLVSKGLKVTLVSTTTSIIQSMHAQASSSITIELLSNELGQQKDESLEAYVERFGIVGSQSLAQLIEKHSRSNSPARILVYDSVILWAQDVADRVGLDAAPFFTQSCAVSAIYYHENHGTFKLPLEGSMISIPSLPPLDTDHDLPSLVKDMDSYPAILKINLNQFSAFHKVKCVFFNTFHKLEHEAVNWMASQWPMIKTVGPTIPSVYLDDRLDQDKGYGLSIFKSTNNTCITWLDTKGISSVVYVSFGSMASLEQEQMEELALGLKRSNTNFLWVVRESEREKLPSNLLEETSEKGLVVSWCPQLEVLSHKAVGCFMTHCGWNSTLEALSLGVPMIAMPRSSDQPTNAKFVQDVWGVGIRAKGDEKGIVKREEIEECIREAMEGEKGNEMKRNALRWKELAKDAVNEGGTSDKNIEEFVALVRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A6F5</accession>
    <name>A0AA39A6F5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Auxin-responsive protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_003878</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Aux/IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations. Repression is thought to result from the interaction with auxin response factors (ARFs), proteins that bind to the auxin-responsive promoter element (AuxRE). Formation of heterodimers with ARF proteins may alter their ability to modulate early auxin response genes expression.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimers and heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the Aux/IAA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9701853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A6F5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000225">
        <property type="entry name" value="Auxin-responsive protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033389">
        <property type="entry name" value="AUX/IAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003311">
        <property type="entry name" value="AUX_IAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31734">
        <property type="entry name" value="AUXIN-RESPONSIVE PROTEIN IAA17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31734:SF2">
        <property type="entry name" value="AUXIN-RESPONSIVE PROTEIN IAA26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02309">
        <property type="entry name" value="AUX_IAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0927">Auxin signaling pathway</keyword>
    <keyword evidence="6 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0678">Repressor</keyword>
    <keyword evidence="5 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="PB1" evidence="11">
        <location>
            <begin position="214"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="18"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="79"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="184"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="27"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="185"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9701853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="345" mass="38004" checksum="24CD8D4C2A18DB6A" modified="2024-01-24" version="1">MEESPQWLNMIPKDREWHARESKRRHGVSEDKKLELRLGPPGEDRSLLSLSYLSSMASITHLHNNSHGAKRGFQDTLEAKPWPRGSLSSSSSAFEKQNHHPKSSYLQYPVVPQTLGAIVDESSKPRPTSMADQAQQYKDKMACSVAADASVSANTAVPNCSQKRIEHAPVVGWPPIRSFRKNLVNSSSSKPASESPNKIPEETGYGKSESSKTGLFVKINMDGVPIGRKVDLKACDSYEKLSYAVDDLFRGLLSAQNESSAGTANESKMEEAKTMAELFDGSGEYTLVYEDNEGDRMLVGDVPWHMFVSTVRRLRVLKSSELAILCVSSSKQEKPPPGSAVEFGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A801</accession>
    <name>A0AA39A801_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_004054</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A801"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052370">
        <property type="entry name" value="Meta-cleavage_hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43139">
        <property type="entry name" value="SI:DKEY-122A22.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43139:SF52">
        <property type="entry name" value="SI:DKEY-122A22.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="50"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9702132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="298" mass="33951" checksum="8F6F5020DC87CC4F" modified="2024-01-24" version="1">MVASCLSPTSLYGGYLRRCFTASGLSSQTIDIDHQTTIHFWGPTTASHKPVLLLVHGFGPVCLWQWRRQVQYFCADFDVYVPDLIFFGDSTTTSSDRTEVFQAVSMGKLLEKVGIERYAVMGTSYGGFVAYHMAYMWPDRVEKVVIASSAVNLIRRDNEELLQRAKLKEIEDLMLPRTAEQLRTLTSLAVFKRLPMIPNFLWNDIIDKIYSDKREEKKGLLKGLTLGREDTPNISPLQQEVLIIWGDHDQIFPLGKAIELKEVLGEKAKLEVMKKTAHMPQVEFPERFNAIVKNFLCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A888</accession>
    <name>A0AA39A888_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">chitinase</fullName>
            <ecNumber evidence="2">3.2.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_006863</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1-&gt;4)-beta-linkages in chitin and chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.14"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.14" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A888"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008843">
        <property type="term" value="F:endochitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016998">
        <property type="term" value="P:cell wall macromolecule catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00325">
        <property type="entry name" value="chitinase_GH19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00035">
        <property type="entry name" value="ChtBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.20.10:FF:000001">
        <property type="entry name" value="Endochitinase (Chitinase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.530.10:FF:000052">
        <property type="entry name" value="Endochitinase PR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.530.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.20.10">
        <property type="entry name" value="Endochitinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.10">
        <property type="entry name" value="Endochitinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001002">
        <property type="entry name" value="Chitin-bd_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018371">
        <property type="entry name" value="Chitin-binding_1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036861">
        <property type="entry name" value="Endochitinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016283">
        <property type="entry name" value="Glyco_hydro_19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000726">
        <property type="entry name" value="Glyco_hydro_19_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22595:SF194">
        <property type="entry name" value="CHITINASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22595">
        <property type="entry name" value="CHITINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00187">
        <property type="entry name" value="Chitin_bind_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00182">
        <property type="entry name" value="Glyco_hydro_19"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001060">
        <property type="entry name" value="Endochitinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00270">
        <property type="entry name" value="ChtBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="Lysozyme-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57016">
        <property type="entry name" value="Plant lectins/antimicrobial peptides"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00026">
        <property type="entry name" value="CHIT_BIND_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50941">
        <property type="entry name" value="CHIT_BIND_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00773">
        <property type="entry name" value="CHITINASE_19_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00774">
        <property type="entry name" value="CHITINASE_19_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7" id="KW-0146">Chitin degradation</keyword>
    <keyword evidence="3 14" id="KW-0147">Chitin-binding</keyword>
    <keyword evidence="8 13 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0611">Plant defense</keyword>
    <keyword evidence="11" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 15" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041353839" description="chitinase" evidence="15">
        <location>
            <begin position="25"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-1" evidence="16">
        <location>
            <begin position="24"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="12">
        <location>
            <position position="133"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13 14">
        <location>
            <begin position="29"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13 14">
        <location>
            <begin position="34"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="89"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="151"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="238"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR001060-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR001060-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9701669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="270" mass="28117" checksum="560068F178A653AB" modified="2024-01-24" version="1">MGAKLLTILLVGVLLAALQGSTVAQNCGCASGLCCSKSGYCGTGNAYCGEGCQAGPCYSSGGSSGGVSVADVVTQAFFDGIINQAAGNCEGKHFYTRAAFLSALNSYPGFGKDGSADQSKREIAAFFAHVTHETGHFCYINEMGGASKNYCNSSNTQNPCVAGQKYYGRGPLQISWNYNYGPAGRSIGFNGLSNPGIVATNVDISFKTALWFWMNSVHPVVNQGFGATIRAINGAIECNGGSSSSVNARVQYYKNYCSQFGVSPGDNLTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A8L3</accession>
    <name>A0AA39A8L3_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_004670</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A8L3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9703008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="179" mass="20632" checksum="36937B4FF04295BD" modified="2024-01-24" version="1">MASNFRAEFRERKHKRLSKSIVINHNSSKKAFSELASAPSLMPVPLAIPTTVTLEPDEKLPSVDDIAYHEMRRPFIVPENLSEESFECMTSSPPYPKSAYVPSQDEIFEFLKRIISFTEREPLIQNMGVFFSTTQRILVEIDDNPNRSFTAQLLYDTPDRLSVAFSICKTTRLLKQQKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A8S8</accession>
    <name>A0AA39A8S8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glutathione transferase</fullName>
            <ecNumber evidence="3">2.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_004680</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GST superfamily. Phi family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A8S8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043295">
        <property type="term" value="F:glutathione binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009407">
        <property type="term" value="P:toxin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03187">
        <property type="entry name" value="GST_C_Phi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03053">
        <property type="entry name" value="GST_N_Phi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000004">
        <property type="entry name" value="Glutathione S-transferase F2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000016">
        <property type="entry name" value="Glutathione S-transferase F2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034347">
        <property type="entry name" value="GST_Phi_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43900:SF54">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE F12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43900">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0216">Detoxification</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="8">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="9">
        <location>
            <begin position="89"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9703022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="213" mass="24149" checksum="CCDF32A7BD74F16D" modified="2024-01-24" version="1">MVVKVYGAVTAACPQRVLACLVEKGVEFEVVHVDLDSGEQKRPEFLIRQPFGQVPVMEDGDFRLFESRAIIRYIAAKYVDEGPNLVGRSLEERGLVDQWLEVEENNLNPLIFTVVLQLVILPRMGEKGDLALVRNCEQKLDKVFDAYEQRLSKSCYLAGDSFTLADLSHLPGIRYLVNEAGMGHLITERKNVNAWWENISNRPAWKKVMNLAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A8W3</accession>
    <name>A0AA39A8W3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Ionotropic glutamate receptor C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_004553</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A8W3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015276">
        <property type="term" value="F:ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015683">
        <property type="entry name" value="Ionotropic_Glu_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001320">
        <property type="entry name" value="Iontro_rcpt_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18966">
        <property type="entry name" value="IONOTROPIC GLUTAMATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00060">
        <property type="entry name" value="Lig_chan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00079">
        <property type="entry name" value="PBPe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="192"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor C-terminal" evidence="12">
        <location>
            <begin position="5"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9702859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="321" mass="35855" checksum="C32899BBA91CAAD4" modified="2024-01-24" version="1">MLTEERIVSNLARIVMIIWFFVVLILTQSYTASLTSRLTVQQLNPTITDINELIKKGEPVGCPNDSFVSEFLIESIKFDESKLVKYESPEELDELFSKGGIAAAFDQIPYMKIFLAKYCSKYTAVGPTYKFDGFGFVFPTGSPLVADVSRKVLSVTEGAKLLEFEKAWFGQTTSCPELTSSVSSNSIGLNSFWGLFLIAGVASFVSLVACITTFLYENRDALINLNPPSSIWRKIKAMATRFDDKDLRSHTLRKSDQLPDKGHQIHGCSYSLASYTNFPPRTPSSGYGDPVSPNRPMSPHILFEYELANINHEQTRPPVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A997</accession>
    <name>A0AA39A997_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Glutamate receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PVL29_004529</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Glutamate-gated receptor that probably acts as a non-selective cation channel. May be involved in light-signal transduction and calcium homeostasis via the regulation of calcium influx into cells.</text>
    </comment>
    <comment type="function">
        <text evidence="15">Glutamate-gated receptor that probably acts as non-selective cation channel.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">May form heteromers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A997"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015276">
        <property type="term" value="F:ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13686">
        <property type="entry name" value="GluR_Plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19990">
        <property type="entry name" value="PBP1_GABAb_receptor_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000037">
        <property type="entry name" value="Glutamate receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000103">
        <property type="entry name" value="Glutamate receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000169">
        <property type="entry name" value="Glutamate receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000195">
        <property type="entry name" value="Glutamate receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000195">
        <property type="entry name" value="Glutamate receptor 2.7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044440">
        <property type="entry name" value="GABAb_receptor_plant_PBP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019594">
        <property type="entry name" value="Glu/Gly-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015683">
        <property type="entry name" value="Ionotropic_Glu_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001320">
        <property type="entry name" value="Iontro_rcpt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017103">
        <property type="entry name" value="Iontropic_Glu_rcpt_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34836">
        <property type="entry name" value="OS06G0188250 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34836:SF1">
        <property type="entry name" value="OS09G0428600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00060">
        <property type="entry name" value="Lig_chan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10613">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037090">
        <property type="entry name" value="Iontro_Glu-like_rcpt_pln"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00079">
        <property type="entry name" value="PBPe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13 15" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 15" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="9 15 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 15" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 15" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="558"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="617"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="801"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor C-terminal" evidence="19">
        <location>
            <begin position="430"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="907"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="728"/>
            <end position="784"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR037090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR037090-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAJ9702830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="953" mass="105112" checksum="1248A8685FA3A34C" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A9R8</accession>
    <name>A0AA39A9R8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_004904</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 17">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PP2C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A9R8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000024">
        <property type="entry name" value="probable protein phosphatase 2C 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="13">
        <location>
            <begin position="63"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="413"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="9"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="23"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="428"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9703294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="487" mass="53811" checksum="B2003D8B2AB0A0CF" modified="2024-01-24" version="1">MGSCLSAESRSPLPSSPSGSLGVRRRKNSKRRLGPRTSPFDSRREEQLHKIPGRMFLNGASDFASLFTQQGKKGTNQDAMIVWENFGSRTDTVFCGVFDGHGPYGHMVAKRVRDSLPLKLSQHWEVNINEDVLKEISLNTTGSMNSEDTAFISADEESRPSFDLEETEKQPEIFQALKESFLKAFKVMDRELRMHTNIDCFCSGTTAVTLVKQGQDLVIGNVGDSRAVLGTRDKDGSLVAIQLTVDLKPNLPGEAERIRRFKGRVFALQDEPEVARVWLPNNDSPGLAMARAFGDFCLKDFGLISVPEISYRRLSEKDEFVVLATDGVWDVLSNKEVVDIVASASLRSTAARTLVETAVRAWRLKYPTSKVDDCAVVCLFLDSNTNNFSTASTKSKEQVTPLEQTDAGIEKEDLLGPTGLDRSGTVRTGKEIHPEDGKEEASKQEEFYTESGIEWSALEGVSRVNTLLTLPRFVPGKDDKKASGKTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ABJ0</accession>
    <name>A0AA39ABJ0_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_002838</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the MIP/aquaporin (TC 1.A.8) family. PIP (TC 1.A.8.11) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ABJ0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015267">
        <property type="term" value="F:channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00333">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1080.10:FF:000001">
        <property type="entry name" value="Probable aquaporin PIP1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034294">
        <property type="entry name" value="Aquaporin_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022357">
        <property type="entry name" value="MIP_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00861">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45687">
        <property type="entry name" value="AQUAPORIN OR AQUAGLYCEROPORIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00221">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="72"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="114"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="194"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="241"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9704461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="279" mass="29637" checksum="88AD5B14EB164E59" modified="2024-01-24" version="1">MSKEVSEEGQSHGKDYVDPPPAPLIDMAELKLWSFHRAVIAEFIATLLFLYITVATVIGYKKQSDPCGGVGLLGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLARKVSLIRALAYMVAQCLGAICGVGLVKAFMKSYYNSLGGGANSVAAGYNKGTALGAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSLGAAVIYNREKVWDDQWIFWVGPFVGALAAAAYHQYILRAAAIKALGSFRSNPTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AC30</accession>
    <name>A0AA39AC30_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GDP-mannose 4,6-dehydratase</fullName>
            <ecNumber evidence="5">4.2.1.47</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_005114</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the conversion of GDP-D-mannose to GDP-4-dehydro-6-deoxy-D-mannose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>GDP-alpha-D-mannose = GDP-4-dehydro-alpha-D-rhamnose + H2O</text>
            <dbReference type="Rhea" id="RHEA:23820"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57527"/>
            <dbReference type="ChEBI" id="CHEBI:57964"/>
            <dbReference type="EC" id="4.2.1.47"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Nucleotide-sugar biosynthesis; GDP-L-fucose biosynthesis via de novo pathway; GDP-L-fucose from GDP-alpha-D-mannose: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the NAD(P)-dependent epimerase/dehydratase family. GDP-mannose 4,6-dehydratase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.47" evidence="5"/>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AC30"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008446">
        <property type="term" value="F:GDP-mannose 4,6-dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042351">
        <property type="term" value="P:'de novo' GDP-L-fucose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05260">
        <property type="entry name" value="GDP_MD_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000102">
        <property type="entry name" value="GDP-mannose 4,6-dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00955">
        <property type="entry name" value="GDP_Man_dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006368">
        <property type="entry name" value="GDP_Man_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01472">
        <property type="entry name" value="gmd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43715:SF1">
        <property type="entry name" value="GDP-MANNOSE 4,6 DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43715">
        <property type="entry name" value="GDP-MANNOSE 4,6-DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16363">
        <property type="entry name" value="GDP_Man_Dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-binding" evidence="10">
        <location>
            <begin position="31"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="8"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00059383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9703678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="373" mass="41867" checksum="C4FE15DD8299B478" modified="2024-01-24" version="1">MASEKETAGSGSAAAINGGEGLEPPKRQIALITGITGQDGSYLTEFLLNKGYEVHGLIRRSSNFNTQRLNHIYIDPHNSHKARMKLHYADLSDASSLRRWIDTIAPDEVYNLAAQSHVAVSFEIPDYTADVVATGALRLLEAVRSHIMATGRSHIRYYQAGSSEMFGSTPPPQSETSPFHPRSPYAASKCAAHWYTVNYREAYGLFACNGILFNHESPRRGENFVTRKITRAVGRIKIGLQSKLFMGNLQASRDWGFAGDYVEAMWMMLQQDKPDDYVVATEESHTVEEFLEVAFGSVGLNWRDHVVIDKRYFRPAEVDNLKGDSSKARKVLGWKPKVDFEQLVKMMVEEDIELAKREKVLVDAGYMDAQQQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AEY0</accession>
    <name>A0AA39AEY0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Basic secretory protease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_003279</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AEY0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007541">
        <property type="entry name" value="Uncharacterised_BSP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33321">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33321:SF12">
        <property type="entry name" value="PLANT BASIC SECRETORY PROTEIN (BSP) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04450">
        <property type="entry name" value="BSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9705148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="168" mass="19209" checksum="CE11F3DA6D64B891" modified="2024-01-24" version="1">MEDLPTKHPEADRKDVQKVTLIIENGNGIAFTSLNNNEIHVNANYLGSYSGDVTREFRGVIYHEMAHIWQWYGNNDTPGGLIEGIADFVRLRADLAPSHWVQPGQGDRWDQGYDVTAWFLDYCNSLRNGFVAELNKKMRKGYSADFFVELLGKTVDQLWSDYKAQYGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AFB5</accession>
    <name>A0AA39AFB5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Type 1 phosphatases regulator ypi1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_001850</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AFB5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008157">
        <property type="term" value="F:protein phosphatase 1 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004865">
        <property type="term" value="F:protein serine/threonine phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011107">
        <property type="entry name" value="PPI_Ypi1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20835:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE PPP1R11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20835">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE PPP1R11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07491">
        <property type="entry name" value="PPI_Ypi1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="8"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9706575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="117" mass="13125" checksum="0B6DE6E2770285B9" modified="2024-01-24" version="1">MAEPTRPSSSSSSSTITTTLTRQNPSPSQLQQQQQQQTTLVLRLNRRKKVSWKDGTVDNEFLQRKSSKKCCIFHKQKPFDEDDSDDDDHHRHHDHGCRKSDGGDQSGEAGSNGCGDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AGA3</accession>
    <name>A0AA39AGA3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_002188</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AGA3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF78">
        <property type="entry name" value="OS12G0587133 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="398"/>
            <end position="678"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9707085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1094" mass="126138" checksum="033C9E32015DC8B0" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AGJ8</accession>
    <name>A0AA39AGJ8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">FAD-binding PCMH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_003800</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oxygen-dependent FAD-linked oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AGJ8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.43.10:FF:000004">
        <property type="entry name" value="Berberine bridge enzyme-like 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.462.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="entry name" value="Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012951">
        <property type="entry name" value="BBE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32448">
        <property type="entry name" value="OS08G0158400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08031">
        <property type="entry name" value="BBE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041367199" description="FAD-binding PCMH-type domain-containing protein" evidence="8">
        <location>
            <begin position="25"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="9">
        <location>
            <begin position="76"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9705869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="540" mass="60733" checksum="E0FF72613311B515" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AGW1</accession>
    <name>A0AA39AGW1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ELYS-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_002091</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AGW1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025151">
        <property type="entry name" value="ELYS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044718">
        <property type="entry name" value="HOS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47358">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HOS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47358:SF2">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HOS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13934">
        <property type="entry name" value="ELYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ELYS-like" evidence="4">
        <location>
            <begin position="333"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="844"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="925"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="850"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="938"/>
            <end position="952"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9706954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="976" mass="109889" checksum="3C5585ABA6ED8247" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AJM3</accession>
    <name>A0AA39AJM3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Auxin efflux carrier component</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_000439</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">May act as a component of the auxin efflux carrier.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the auxin efflux carrier (TC 2.A.69.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AJM3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010329">
        <property type="term" value="F:auxin efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009926">
        <property type="term" value="P:auxin polar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014024">
        <property type="entry name" value="Auxin_eff_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051107">
        <property type="entry name" value="Auxin_Efflux_Carrier"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004776">
        <property type="entry name" value="Mem_transp_PIN-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00946">
        <property type="entry name" value="2a69"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31752">
        <property type="entry name" value="AUXIN EFFLUX CARRIER COMPONENT 1B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31752:SF72">
        <property type="entry name" value="AUXIN EFFLUX CARRIER COMPONENT 3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03547">
        <property type="entry name" value="Mem_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0927">Auxin signaling pathway</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="71"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="104"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="132"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="559"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="583"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="618"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="269"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="330"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="440"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="336"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9708394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="700" mass="77170" checksum="127E3A7E58629CEA" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AKV6</accession>
    <name>A0AA39AKV6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Histidine kinase/HSP90-like ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_001098</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the heat shock protein 90 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AKV6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16927">
        <property type="entry name" value="HATPase_Hsp90-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11260:FF:000005">
        <property type="entry name" value="Heat shock protein 90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.790:FF:000001">
        <property type="entry name" value="Heat shock protein 90 alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.80:FF:000005">
        <property type="entry name" value="heat shock protein 90-5, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000024">
        <property type="entry name" value="heat shock protein 90-5, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.790">
        <property type="entry name" value="Heat shock protein 90, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00505">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019805">
        <property type="entry name" value="Heat_shock_protein_90_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037196">
        <property type="entry name" value="HSP90_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001404">
        <property type="entry name" value="Hsp90_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020575">
        <property type="entry name" value="Hsp90_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003555">
        <property type="entry name" value="PRK05218.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11528">
        <property type="entry name" value="HEAT SHOCK PROTEIN 90 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13589">
        <property type="entry name" value="HATPase_c_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00183">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002583">
        <property type="entry name" value="Hsp90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00775">
        <property type="entry name" value="HEATSHOCK90"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110942">
        <property type="entry name" value="HSP90 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00298">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0346">Stress response</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Histidine kinase/HSP90-like ATPase" evidence="9">
        <location>
            <begin position="102"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="298"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="745"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="310"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="751"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="782"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="175"/>
            <end position="176"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="199"/>
            <end position="204"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="446"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR002583-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9709457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="793" mass="90704" checksum="95EC2F701F6A9547" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D407</accession>
    <name>A0AA39D407_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Calmodulin-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_027234</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9671153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D407"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012417">
        <property type="entry name" value="CaM-bd_dom_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044681">
        <property type="entry name" value="PICBP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33923">
        <property type="entry name" value="CALMODULIN-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33923:SF2">
        <property type="entry name" value="CALMODULIN-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07839">
        <property type="entry name" value="CaM_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01054">
        <property type="entry name" value="CaM_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calmodulin-binding" evidence="2">
        <location>
            <begin position="827"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="341"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="509"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="638"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="716"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="848"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="124"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="366"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="442"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="466"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="523"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="638"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="666"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="752"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="811"/>
            <end position="821"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9671153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="968" mass="106521" checksum="344FE8BA8A3B3831" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D5J4</accession>
    <name>A0AA39D5J4_VITRO</name>
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        <recommendedName>
            <fullName evidence="2">Knr4/Smi1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_026962</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
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            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D5J4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018958">
        <property type="entry name" value="Knr4/Smi1-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32011">
        <property type="entry name" value="OS08G0472400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32011:SF2">
        <property type="entry name" value="OS08G0472400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00860">
        <property type="entry name" value="SMI1_KNR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Knr4/Smi1-like" evidence="2">
        <location>
            <begin position="66"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="217"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9670750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="397" mass="43599" checksum="100D7DDD335E7EE2" modified="2024-01-24" version="1">MVDVDRRMTGLNPAHIAGLRRLSARAAAPSTVSTALPVRNGLISFTTLADKVIAHLQNSGIQVQAGLSDAEFARAEAEFGFAFPPDLKAVLSAGLPVGPGFPDWRAAGARLRLRASLDLPIAAISFQIARNSLWSKSWGPRPSDPEKALRIARNALKRAPLLIPIFNHCYIPCNPSLAGNPIFFVDENRIFCCGLDLSDFFERESLFQSSDPQVLKKQRSVSEKTAGSSSNFSRRSLDSGGLAGGRTPRWVEFWSDAAIDRRRRNSSSSSSSSPERFFDMPRSEIPKWVDEYIEQIGSVLREGGWDESDIAEIVTVSASGFFEGEMVLLDNQAVLDALLLKADRFSDSLRKAGWSSEEVSDALGFDYRPEKERKPAKKLAPELVERIGKLAESVSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D5R3</accession>
    <name>A0AA39D5R3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_026282</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D5R3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003441">
        <property type="entry name" value="NAC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036093">
        <property type="entry name" value="NAC_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31744">
        <property type="entry name" value="PROTEIN CUP-SHAPED COTYLEDON 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31744:SF65">
        <property type="entry name" value="TRANSCRIPTION FACTOR JUNGBRUNNEN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02365">
        <property type="entry name" value="NAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101941">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51005">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NAC" evidence="5">
        <location>
            <begin position="1"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9672953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="258" mass="28971" checksum="45C5746A073F6644" modified="2024-01-24" version="1">MGSLNSKLSHDSVVLISLVANLDGTVTDKEWYFFCRRGRKYRNRVRPNRVTGSGFWKATGIDKPIYSVEEGHSRGDCIGLKKSLVYYCGSAGKGTKTDWMMHEFRLPADQKNATLPTAKAADQEAEVWTLCRIFKRNVSHRRYTPDWREVPAKRNPTNSKPKTCDMSPENWERYISFGAPVSLHKGKKPVGSHIDEGNPLPAGQVSSIVQGSQPESCSSGILSNDAREFFAHGNWDEIVSVMELSFDPSSINNFKIMH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D7Z3</accession>
    <name>A0AA39D7Z3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NAB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_023523</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D7Z3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011684">
        <property type="entry name" value="NAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056889">
        <property type="entry name" value="NET2A-D/KIP1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056888">
        <property type="entry name" value="NET2A-D/KIP1-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31631:SF3">
        <property type="entry name" value="PROTEIN NETWORKED 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31631">
        <property type="entry name" value="PROTEIN NETWORKED 2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07765">
        <property type="entry name" value="KIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25014">
        <property type="entry name" value="NET2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24918">
        <property type="entry name" value="NET2A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51774">
        <property type="entry name" value="NAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAB" evidence="4">
        <location>
            <begin position="10"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="499"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="692"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="177"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="411"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="820"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="541"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="560"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="578"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="606"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="692"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="729"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="770"/>
            <end position="779"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9674019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1024" mass="117213" checksum="6C4AFAC3F61F05EC" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D8T6</accession>
    <name>A0AA39D8T6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GAG-pre-integrase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_024063</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D8T6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025724">
        <property type="entry name" value="GAG-pre-integrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054722">
        <property type="entry name" value="PolX-like_BBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34222">
        <property type="entry name" value="GAG_PRE-INTEGRS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34222:SF95">
        <property type="entry name" value="RRNA 2'-O-METHYLTRANSFERASE FIBRILLARIN-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13976">
        <property type="entry name" value="gag_pre-integrs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22936">
        <property type="entry name" value="Pol_BBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Retrovirus-related Pol polyprotein from transposon TNT 1-94-like beta-barrel" evidence="3">
        <location>
            <begin position="196"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="GAG-pre-integrase" evidence="2">
        <location>
            <begin position="302"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="143"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9674919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="395" mass="42804" checksum="5BCED3005B2B34A6" modified="2024-01-24" version="1">MDLFTYIGQIASLKEEFLTVMPLTTDVGDQRTQINKFFMVLTLIGLRPNLKTVCDQILGSSSVPSLDDVFARLLRISSTQPLSSDNTLDSSVLVSQTNSRGGRNGNRGRGQRPHCTYCNKLGHTRDRCYQLHGQPPRTAHVAQSSDPQPPQLSSSSTSQGISLTDSEYDQATKSASVAQTGNASDCLTHTSSLGPWILDSSASDHISSNKDLFSSITTTSALPTVTLANGSQTMAKGIGLAHPLPSLPLTSVLYTPECSFNLISISKITRTLNCSITFSDKFVNLQDWRTRKMIGIGRESQGLYHLTSPSTPTVCISTDAPLLIHSCLGHLSLSKFQKMVPCFSSLSSLACESCQLGKHTRVSFPKRLNNQAKSPFELVHTDVWGPCQTASTLGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DAE4</accession>
    <name>A0AA39DAE4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tr-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_022062</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9676871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DAE4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01883">
        <property type="entry name" value="EF1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03704">
        <property type="entry name" value="eRF3_C_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04089">
        <property type="entry name" value="eRF3_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000024">
        <property type="entry name" value="Eukaryotic peptide chain release factor GTP-binding subunit ERF3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000862">
        <property type="entry name" value="Eukaryotic peptide chain release factor GTP-binding subunit ERF3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000077">
        <property type="entry name" value="Putative translation elongation/initiation factor family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054696">
        <property type="entry name" value="GTP-eEF1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050100">
        <property type="entry name" value="TRAFAC_GTPase_members"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23115">
        <property type="entry name" value="TRANSLATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22594">
        <property type="entry name" value="GTP-eEF1A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="7">
        <location>
            <begin position="80"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="27"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="65"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9676871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="512" mass="57054" checksum="CDF609C46908EF8A" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DCA8</accession>
    <name>A0AA39DCA8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_020897</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Functions as an E3 ubiquitin ligase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DCA8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16655">
        <property type="entry name" value="RING-Ubox_WDSUB1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01989">
        <property type="entry name" value="USP_STK_Ubox_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051348">
        <property type="entry name" value="U-box_ubiquitin_ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45647">
        <property type="entry name" value="OS02G0152300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45647:SF15">
        <property type="entry name" value="U-BOX DOMAIN-CONTAINING PROTEIN 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04564">
        <property type="entry name" value="U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51698">
        <property type="entry name" value="U_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="452"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="domain" description="U-box" evidence="15">
        <location>
            <begin position="732"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="177"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="353"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="184"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="198"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="479"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9678836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="804" mass="90123" checksum="ADF189847C1F798A" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DDK6</accession>
    <name>A0AA39DDK6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">PVL29_021657</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="23">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DDK6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000590">
        <property type="entry name" value="PR5-like receptor kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000059">
        <property type="entry name" value="S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045874">
        <property type="entry name" value="LRK10/LRL21-25-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025287">
        <property type="entry name" value="WAK_GUB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27009">
        <property type="entry name" value="RUST RESISTANCE KINASE LR10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13947">
        <property type="entry name" value="GUB_WAK_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="12 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7 21" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041321711" description="non-specific serine/threonine protein kinase" evidence="21">
        <location>
            <begin position="27"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="238"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="299"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="573"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="580"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAJ9679809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="606" mass="68770" checksum="AC4FA43A98F84A5C" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DE42</accession>
    <name>A0AA39DE42_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 4">3-hydroxyisobutyryl-CoA hydrolase</fullName>
            <shortName evidence="4">HIB-CoA hydrolase</shortName>
            <shortName evidence="4">HIBYL-CoA-H</shortName>
            <ecNumber evidence="2 4">3.1.2.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">3-hydroxyisobutyryl-coenzyme A hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_019629</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Hydrolyzes 3-hydroxyisobutyryl-CoA (HIBYL-CoA), a saline catabolite. Has high activity toward isobutyryl-CoA. Could be an isobutyryl-CoA dehydrogenase that functions in valine catabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 4">
            <text>3-hydroxy-2-methylpropanoyl-CoA + H2O = 3-hydroxy-2-methylpropanoate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20888"/>
            <dbReference type="ChEBI" id="CHEBI:11805"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57340"/>
            <dbReference type="EC" id="3.1.2.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid degradation; L-valine degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.4" evidence="2 4"/>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DE42"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003860">
        <property type="term" value="F:3-hydroxyisobutyryl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006574">
        <property type="term" value="P:L-valine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045004">
        <property type="entry name" value="ECH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032259">
        <property type="entry name" value="HIBYL-CoA-H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43176:SF3">
        <property type="entry name" value="3-HYDROXYISOBUTYRYL-COA HYDROLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43176">
        <property type="entry name" value="3-HYDROXYISOBUTYRYL-COA HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16113">
        <property type="entry name" value="ECH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl-CoA hydratase/isomerase" evidence="5">
        <location>
            <begin position="112"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU369070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF16113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9680365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="355" mass="39570" checksum="20421AE10AE26ADA" modified="2024-01-24" version="1">MAPPGSTNGDGEQVLVEENLFLRTLILNRPRQLNALSFQMVSKLLELFVAYEDDCNVKLVILKGNGRAFCAGGDVATVVHDINGGDWRLGAKFFWKEFILNYVMATYSKPQVFAMPETALGLFPDVGASYFLSRLPGFFGEYVGLTGARLDGAEMLACGLATHFVPSARLSLLEAALCKLDSGAPATISSILDEYSQQPHLKENSAHHKLAIINRCFSRRTIEEIISTLEREAVNSADNWISATIQSLQKASPTSLKISLRSIREGRLQDVGQCLTREYRMVCHVLQGEVSKDFFEGCRAILLDKDKNPKWEPSRLEQINESMVEQYFSKVDDEEWEDLKLPAKFTFSASSLAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DGK2</accession>
    <name>A0AA39DGK2_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_016563</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DGK2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007750">
        <property type="entry name" value="DUF674"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33103">
        <property type="entry name" value="OS01G0153900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33103:SF19">
        <property type="entry name" value="OS09G0544700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05056">
        <property type="entry name" value="DUF674"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9684128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="105" mass="11756" checksum="250D3954A00DCFE3" modified="2024-01-24" version="1">MDSQANYIFSDTEKAEEGYVKGAVTYMVTDALVVKPLSTTSIVDLLSKSNINEVGGELEEKVVDFTMREALLLETLISLLTKRSKEAWLCEIFLENIAYTIAKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DHK2</accession>
    <name>A0AA39DHK2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_018377</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057135">
        <property type="entry name" value="At4g27190-like_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050905">
        <property type="entry name" value="Plant_NBS-LRR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463:SF198">
        <property type="entry name" value="RPP4C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23247">
        <property type="entry name" value="LRR_RPS2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NB-ARC" evidence="6">
        <location>
            <begin position="157"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein At4g27190-like leucine-rich repeats" evidence="7">
        <location>
            <begin position="801"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein At4g27190-like leucine-rich repeats" evidence="7">
        <location>
            <begin position="929"/>
            <end position="1017"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein At4g27190-like leucine-rich repeats" evidence="7">
        <location>
            <begin position="1082"/>
            <end position="1188"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein At4g27190-like leucine-rich repeats" evidence="7">
        <location>
            <begin position="1224"/>
            <end position="1299"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein At4g27190-like leucine-rich repeats" evidence="7">
        <location>
            <begin position="1410"/>
            <end position="1536"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein At4g27190-like leucine-rich repeats" evidence="7">
        <location>
            <begin position="1540"/>
            <end position="1641"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="27"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9682447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1704" mass="192679" checksum="2CB75280FBDAFAD5" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DIT1</accession>
    <name>A0AA39DIT1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FMR1-interacting protein 1 conserved domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_017268</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DIT1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000492">
        <property type="term" value="P:box C/D snoRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039136">
        <property type="entry name" value="NUFIP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019496">
        <property type="entry name" value="NUFIP1_cons_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13309:SF0">
        <property type="entry name" value="FMR1-INTERACTING PROTEIN NUFIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13309">
        <property type="entry name" value="NUCLEAR FRAGILE X MENTAL RETARDATION PROTEIN INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10453">
        <property type="entry name" value="NUFIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMR1-interacting protein 1 conserved" evidence="2">
        <location>
            <begin position="319"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="251"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="403"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="536"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="405"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="442"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="569"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="583"/>
            <end position="598"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9685165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="598" mass="67121" checksum="700FE73B4DDF4609" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DJ36</accession>
    <name>A0AA39DJ36_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Beta-amylase</fullName>
            <ecNumber evidence="3 10">3.2.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_015550</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>Hydrolysis of (1-&gt;4)-alpha-D-glucosidic linkages in polysaccharides so as to remove successive maltose units from the non-reducing ends of the chains.</text>
            <dbReference type="EC" id="3.2.1.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the glycosyl hydrolase 14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.2" evidence="3 10"/>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DJ36"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016161">
        <property type="term" value="F:beta-amylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000066">
        <property type="entry name" value="Beta-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001554">
        <property type="entry name" value="Glyco_hydro_14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018238">
        <property type="entry name" value="Glyco_hydro_14_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001371">
        <property type="entry name" value="Glyco_hydro_14B_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31352">
        <property type="entry name" value="BETA-AMYLASE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31352:SF40">
        <property type="entry name" value="BETA-AMYLASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01373">
        <property type="entry name" value="Glyco_hydro_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00750">
        <property type="entry name" value="BETAAMYLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00842">
        <property type="entry name" value="GLHYDLASE14B"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00506">
        <property type="entry name" value="BETA_AMYLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00679">
        <property type="entry name" value="BETA_AMYLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6 10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 10" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="192"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="386"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="301"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="348"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="387"/>
            <end position="388"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="426"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601554-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR601554-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9686753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="520" mass="58118" checksum="45737CBC572E9275" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DJT0</accession>
    <name>A0AA39DJT0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cupin type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_016066</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 11S seed storage protein (globulins) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DJT0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045735">
        <property type="term" value="F:nutrient reservoir activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02243">
        <property type="entry name" value="cupin_11S_legumin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02242">
        <property type="entry name" value="cupin_11S_legumin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006044">
        <property type="entry name" value="11S_seedstore_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006045">
        <property type="entry name" value="Cupin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050253">
        <property type="entry name" value="Seed_Storage-Functional"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31189">
        <property type="entry name" value="OS03G0336100 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31189:SF45">
        <property type="entry name" value="OS09G0552500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00190">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00439">
        <property type="entry name" value="11SGLOBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00835">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0708">Seed storage protein</keyword>
    <keyword evidence="2" id="KW-0758">Storage protein</keyword>
    <feature type="domain" description="Cupin type-1" evidence="5">
        <location>
            <begin position="3"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="Cupin type-1" evidence="5">
        <location>
            <begin position="192"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9687428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="358" mass="38704" checksum="7EA116E1774F94AA" modified="2024-01-24" version="1">MELNLAPKFAQKIFEGEGGTYYSWSSAEYELLKEAKVGGGRLVLQPRGFALPHYADSNKIGYVLQGRCGVVGMVSPKASEEVVLKLKEGDIIPVPYGAVSWWYNDGDSELVIVFLGETSKAYVPGEFTYFLLTGTQGILGGFSTEFNSRAYNINNEEAKKLAKSQTGVLLIKIPEGQNMPQPCKNSSDKLVYNIDAALPDVHVQNAGLLTALTAKKFPFLREVGLSATLVKLDANAMSSPIYAADSSVQVIYVAKGSGRIQVVGINGERALDTKVKAGHLCVVPRFFVASVSADGEGIEYFSMITTTQPVFGEFTGKTSVWGALSPKVLQASLNVGPEFEQLFRAKIKKSTILVPPQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DLX1</accession>
    <name>A0AA39DLX1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">N-alpha-acetyltransferase 35, NatC auxiliary subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_014401</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAK10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DLX1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031417">
        <property type="term" value="C:NatC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057983">
        <property type="entry name" value="NAA35-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007244">
        <property type="entry name" value="Naa35_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057982">
        <property type="entry name" value="TPR_NAA35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21373">
        <property type="entry name" value="GLUCOSE REPRESSIBLE PROTEIN MAK10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21373:SF0">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 35, NATC AUXILIARY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04112">
        <property type="entry name" value="Mak10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25789">
        <property type="entry name" value="TPR_NAA35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAA35-like N-terminal" evidence="5">
        <location>
            <begin position="45"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="NAA35-like TPR repeats" evidence="6">
        <location>
            <begin position="354"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9688734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="728" mass="82665" checksum="967875CEF9DC89B6" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DMA0</accession>
    <name>A0AA39DMA0_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_012448</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the iron-sulfur cluster assembly SufBD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9689771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DMA0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055346">
        <property type="entry name" value="Fe-S_cluster_assembly_SufBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010231">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000825">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037284">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045595">
        <property type="entry name" value="SufBD_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008773">
        <property type="entry name" value="PRK11814.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01980">
        <property type="entry name" value="sufB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30508">
        <property type="entry name" value="FES CLUSTER ASSEMBLY PROTEIN SUF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30508:SF1">
        <property type="entry name" value="UPF0051 PROTEIN ABCI8, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01458">
        <property type="entry name" value="SUFBD_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19295">
        <property type="entry name" value="SufBD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101960">
        <property type="entry name" value="Stabilizer of iron transporter SufD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SUF system FeS cluster assembly SufBD N-terminal" evidence="4">
        <location>
            <begin position="207"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="SUF system FeS cluster assembly SufBD core" evidence="3">
        <location>
            <begin position="283"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00043967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF19295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9689771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="554" mass="62072" checksum="0C5187D91365E84D" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DMG2</accession>
    <name>A0AA39DMG2_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_012580</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9689981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DMG2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900150">
        <property type="term" value="P:regulation of defense response to fungus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044169">
        <property type="entry name" value="PI21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47488">
        <property type="entry name" value="HEAVY METAL TRANSPORT/DETOXIFICATION SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47488:SF7">
        <property type="entry name" value="HEAVY METAL TRANSPORT_DETOXIFICATION SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9689981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="243" mass="27490" checksum="689A0ACBEB48EE3F" modified="2024-01-24" version="1">MSEVTTMRLRVDLECDRCYKKIKKLLCKFPEIRDQIFYEKDNTVEIKVVCCSPGKIRDKLICKGGKTIKSIEIIVPEKPKTPSEKPKPPPEKPKEAEKPKPLPERPKEPEKPKPPPEKPKEPEKLKPPPEKKEEPKKEEPKDQPKPVVKPPAPAPAPAPVPGYPQFYPVAVCCKPCFDLGHGGPCHHGNGIPYQRPSTYDGYVRLVPSYDEPYGGWPSGCRCNRSYGCRCEYFTEENPACTIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DNF0</accession>
    <name>A0AA39DNF0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SIT4 phosphatase-associated family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_013366</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SAPS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DNF0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019903">
        <property type="term" value="F:protein phosphatase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007587">
        <property type="entry name" value="SAPS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12634:SF37">
        <property type="entry name" value="SIT4 PHOSPHATASE-ASSOCIATED FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12634">
        <property type="entry name" value="SIT4 YEAST -ASSOCIATING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04499">
        <property type="entry name" value="SAPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="597"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="597"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="623"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="654"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="738"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="777"/>
            <end position="790"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9691163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="790" mass="88268" checksum="287F365588705B16" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DQJ5</accession>
    <name>A0AA39DQJ5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_011519</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DQJ5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03232">
        <property type="entry name" value="ABCG_PDR_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000059">
        <property type="entry name" value="ABC transporter G family member 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034003">
        <property type="entry name" value="ABCG_PDR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48040:SF20">
        <property type="entry name" value="PLEIOTROPIC DRUG RESISTANCE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48040">
        <property type="entry name" value="PLEIOTROPIC DRUG RESISTANCE PROTEIN 1-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="175"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="208"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="254"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="293"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="318"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="744"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="784"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="827"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="857"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="382"/>
            <end position="635"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9692499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="898" mass="101306" checksum="1BA3155BFFDB47A9" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DRA0</accession>
    <name>A0AA39DRA0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Putative plant transposon protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_009886</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DRA0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046796">
        <property type="entry name" value="Transposase_32_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20167">
        <property type="entry name" value="Transposase_32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Putative plant transposon protein" evidence="2">
        <location>
            <begin position="96"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9694118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="354" mass="39287" checksum="4C38705CFA95D283" modified="2024-01-24" version="1">MPPKGRKRKAPVKGSSSKTSKGARNENLSLIPHVQTPSIPKAPAKTSIDGIAFNAFQAKFYRDQYNKVYRQRSLVLERQLHLESFLDTPIPQLFVELGWLPLANFTRVACAPIVRMFYSNIIEHDLDESYLQSSMFGIVVKVTPEIIAEVLGIPLVEAPSICDLEITSKLLDRVSIDLWGEVRGQLGSVVHTGSISRPAWVLATLLSFSVYPSSHRATICKKGCFLLSRILHREPINLASCILDEMIVRGDPTVSKKEIKGALSSKKKVGHSKAKRKFAGSDSDSDVDSSVLAQLCAKFDALDEKISAQSTLLSEQISQLQSHMERGFSLIDGQMFCNMDQLHALEDFVHKHLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DS07</accession>
    <name>A0AA39DS07_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Tubulin beta chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_010375</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11 12">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="12">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the tubulin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DS07"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02187">
        <property type="entry name" value="beta_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000002">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000002">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000005">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013838">
        <property type="entry name" value="Beta-tubulin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002453">
        <property type="entry name" value="Beta_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01163">
        <property type="entry name" value="BETATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00228">
        <property type="entry name" value="TUBULIN_B_AUTOREG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9 12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 12" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="222"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="15">
        <location>
            <begin position="47"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="16">
        <location>
            <begin position="270"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="446"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="453"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9694868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="471" mass="53240" checksum="13F0108E1FE10C19" modified="2024-01-24" version="1">MREILHIQGGQCGNQIGAKFWEVVCAEHGIDVTGRYQGDSELQLERINVYYNEASCGRFVPRAVLMDLEPGTMDSIRSGMYGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELIDAVLDVVRKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTTPSWCFFLSFSFSFFVTQLIIMILCWAVGDLNHLISATMSGVTCCLRFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTSRGSQQYRALTVPELTQQMWDSKNMMCAADPRHGRYLTASAMFRGKMSTKEVDEQILNVQNKNSSYFVEWIPNNVKSTVCDIPPTGLKMASTFIGNSTSIQEMFRRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVAEYQQYQDATADEEGDYEDEEEEELHDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DTX7</accession>
    <name>A0AA39DTX7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_008316</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
        <text evidence="7">Binds 1 [2Fe-2S] cluster.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I 24 kDa subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9695994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DTX7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003954">
        <property type="term" value="F:NADH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006120">
        <property type="term" value="P:mitochondrial electron transport, NADH to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03064">
        <property type="entry name" value="TRX_Fd_NuoE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000097">
        <property type="entry name" value="NADH dehydrogenase [ubiquinone] flavoprotein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1590:FF:000001">
        <property type="entry name" value="NADH-quinone oxidoreductase subunit E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1590">
        <property type="entry name" value="NADH-quinone oxidoreductase subunit E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002023">
        <property type="entry name" value="NuoE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042128">
        <property type="entry name" value="NuoE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041921">
        <property type="entry name" value="NuoE_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01958">
        <property type="entry name" value="nuoE_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10371:SF3">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] FLAVOPROTEIN 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10371">
        <property type="entry name" value="NADH DEHYDROGENASE UBIQUINONE FLAVOPROTEIN 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01257">
        <property type="entry name" value="2Fe-2S_thioredx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000216">
        <property type="entry name" value="NADH_DH_24kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01099">
        <property type="entry name" value="COMPLEX1_24K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="4 7" id="KW-0408">Iron</keyword>
    <keyword evidence="5 7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="214"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000216-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9695994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="254" mass="28479" checksum="3AF0BCD102E4C9F8" modified="2024-01-24" version="1">MLARLATKRLLEVRQIFRQNHQTSRSFSTALNYHLDSPDNNPSIPWEFTNANKEKVKEILSHYPSNYKQSAVIPLLDLAQQQHGGWLPVSAMDAVAKVIEVAPIRVYEVATFYSMFNRAKVGKYHLLVCGTTPCMIRGSREIEDALLKHLGVKRNEVTKDGLFSVGEMECMGCCVNAPMITVADYSTGSEGYTYNYYEDLTPKRVVEIVEMLRRGEKPPHGTQNPQRTRCGPEGGNTTLLGEPKAPPCRDLDAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DUZ4</accession>
    <name>A0AA39DUZ4_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_008640</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DUZ4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36775">
        <property type="entry name" value="LYR MOTIF PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36775:SF1">
        <property type="entry name" value="LYR MOTIF PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9696520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="166" mass="18111" checksum="90F105DDB1AB1B4C" modified="2024-01-24" version="1">MFDGSPGSAVAVGRGRFLGGVVTAAGLAGAARWGRRRHSSGELFVNGDSNWASDVSEAKNSRKDRDGGSGEKENLGSGFGHIGIFETQGNESGYGSEPGYRGDAEFGYGDELDEEEDDARLLFWGEQLGDNDTNMEMVGENTFSEQKAHHRCRRKKHDYRMIDALR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DV46</accession>
    <name>A0AA39DV46_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_007270</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DV46"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000395">
        <property type="entry name" value="receptor-like serine/threonine-protein kinase NCRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000415">
        <property type="entry name" value="receptor-like serine/threonine-protein kinase NCRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47989">
        <property type="entry name" value="OS01G0750732 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47989:SF23">
        <property type="entry name" value="RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE NCRK ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="15" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041250365" description="non-specific serine/threonine protein kinase" evidence="15">
        <location>
            <begin position="24"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="120"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="230"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9698078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="635" mass="69517" checksum="F70A7CE7A7DDE62D" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DVT2</accession>
    <name>A0AA39DVT2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_007744</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DVT2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057192">
        <property type="entry name" value="DUF7870"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44843">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44843:SF14">
        <property type="entry name" value="METHYLTRANSFERASE TYPE 11 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25276">
        <property type="entry name" value="DUF7870"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="Methyltransferase type 11" evidence="2">
        <location>
            <begin position="232"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7870" evidence="3">
        <location>
            <begin position="360"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7870" evidence="3">
        <location>
            <begin position="497"/>
            <end position="594"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9698813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="594" mass="67897" checksum="0E8B0BC1367AB0DF" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DW40</accession>
    <name>A0AA39DW40_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Retrovirus-related Pol polyprotein from transposon TNT 1-94</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_007372</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DW40"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9698259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="123" mass="14261" checksum="8C073A27340B5913" modified="2024-01-24" version="1">MQEDKNLDENFDEFNKLVIELENIGEKINDEDQIIILLNSLPLTYSQLHDTIKYGRDTLSLDYLEPTIRSKELELRAKKKTNNSNETLNIRGRIEKKNNKNKGKLISKSRESSGVIIATRRVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DY85</accession>
    <name>A0AA39DY85_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DYW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_005990</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. PCMP-H subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DY85"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031425">
        <property type="term" value="P:chloroplast RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001050">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At2g33760"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001630">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At5g43790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000231">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032867">
        <property type="entry name" value="DYW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046849">
        <property type="entry name" value="E2_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF450">
        <property type="entry name" value="DYW DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14432">
        <property type="entry name" value="DYW_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20430">
        <property type="entry name" value="Eplus_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="283"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="DYW" evidence="4">
        <location>
            <begin position="498"/>
            <end position="590"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9700464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="590" mass="66159" checksum="1B7AD555495EB57D" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DZC6</accession>
    <name>A0AA39DZC6_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_006360</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DZC6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9700990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="60" mass="6514" checksum="5D733408759B7F00" modified="2024-01-24" version="1">MDVELTVVMWPTEMIYDSTLIAIGIPNAPAPTVLWPVETKNDLSSTRIGMLDSLVEVAAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DZY7</accession>
    <name>A0AA39DZY7_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_004105</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DZY7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043376">
        <property type="entry name" value="NPG1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44102">
        <property type="entry name" value="PROTEIN NPG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44102:SF5">
        <property type="entry name" value="PROTEIN NPG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="586"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="656"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="93"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9702204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="698" mass="77056" checksum="B45D1B4721C1BF2B" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E0G9</accession>
    <name>A0AA39E0G9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_003190</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Potential calcium sensor.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E0G9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000089">
        <property type="entry name" value="calmodulin-like protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039647">
        <property type="entry name" value="EF_hand_pair_protein_CML-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10891">
        <property type="entry name" value="EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00450">
        <property type="entry name" value="RECOVERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="42"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="78"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="113"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="149"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9705026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="186" mass="21276" checksum="681207ADAB0FE6B3" modified="2024-01-24" version="1">MSTPSFLDFQYNISKRKYLRKPSRLFSSRDRQNSDLAPIFHLNMDELKKVFDKFDSNKDGKISQEEYKAVVRALVKEGVRTEVEKIFQVADLDGDGFIDFKEFVEVHKKGGGVKTRDIQSAFRAFDLNGDGKINAEELLEVLTRLGERCSLEECRRMVRGVDNDGDGAVDIDEFTTMMTRSMKPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E0Q7</accession>
    <name>A0AA39E0Q7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">procollagen-proline 4-dioxygenase</fullName>
            <ecNumber evidence="4">1.14.11.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PVL29_005197</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-prolyl-[collagen] + 2-oxoglutarate + O2 = trans-4-hydroxy-L-prolyl-[collagen] + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:18945"/>
            <dbReference type="Rhea" id="RHEA-COMP:11676"/>
            <dbReference type="Rhea" id="RHEA-COMP:11680"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:50342"/>
            <dbReference type="ChEBI" id="CHEBI:61965"/>
            <dbReference type="EC" id="1.14.11.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the P4HA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.2" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E0Q7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004656">
        <property type="term" value="F:procollagen-proline 4-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.620:FF:000002">
        <property type="entry name" value="Prolyl 4-hydroxylase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045054">
        <property type="entry name" value="P4HA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044862">
        <property type="entry name" value="Pro_4_hyd_alph_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869:SF123">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13640">
        <property type="entry name" value="2OG-FeII_Oxy_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00702">
        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0408">Iron</keyword>
    <keyword evidence="13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="17">
        <location>
            <begin position="160"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ9703823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="288" mass="32283" checksum="E850B59DE44BD3B3" modified="2024-01-24" version="1">MAKPRYPRIPPRKSSSSTVVLSMLLMFSFVVLVLLALGLLSIPSHSGDSPRANDLSTIVQRTVEKSDGNDGKAEPWSEVISWEPRAFVYHNFLSKDECEYLIKLAKPHMHKSTVVDSSTGKSKDSRVRTSSGTFLTRGQDKIIRGIEKRLADFTFLPVEHGEGLQILHYEVGQKYEPHYDYFLDDYNTKNGGQRMATVLMYLSDVEEGGETVFPAAKGNFSSVPWWDELSECGKEGLSVKPKMGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSSTKWIRVNEYKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E1M8</accession>
    <name>A0AA39E1M8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CASP-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_003088</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3 8">Homodimer and heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Casparian strip membrane proteins (CASP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E1M8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006702">
        <property type="entry name" value="CASP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045009">
        <property type="entry name" value="CASPL-5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32021">
        <property type="entry name" value="CASP-LIKE PROTEIN 5B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32021:SF32">
        <property type="entry name" value="CASP-LIKE PROTEIN 5C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04535">
        <property type="entry name" value="CASP_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="78"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="126"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Casparian strip membrane protein" evidence="9">
        <location>
            <begin position="8"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9704873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="152" mass="16640" checksum="84EF82E69A8DE46A" modified="2024-01-24" version="1">MDEVPGSVGTSASFSLRLGQTIFSSASLLFMSLGVEFYSYTAFCYLVTIMGLVIPWSFTLALVDGYSVLVKCPLRQPGILLIIVLGDWVLSILTLAAASSTVGVVDVLLKSEASYYPIKFCTRYQISAAMAFLSWFLSFGSFLFNLWLLPTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E1S2</accession>
    <name>A0AA39E1S2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF829 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_003180</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E1S2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008547">
        <property type="entry name" value="DUF829_TMEM53"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12265:SF9">
        <property type="entry name" value="DUF829 DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12265">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05705">
        <property type="entry name" value="DUF829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="323"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9705008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="407" mass="45478" checksum="40C85ABC8862DE7A" modified="2024-01-24" version="1">MWRDGGKLYWARKEEAVKVEGVVVIFAWASIQETHLKEFVDLYSSLGWNSLVCHAGFLNAFFPEKATSLAFVALKELVEELRIRPCPIVFAAFSGGPKACMYKVFQIIEGSCEAHLHMDDSRLVRNCISGYIYDSSPVDFTSDFGARFGLPPTILKMPGSTKLASWVAKGVASGLDALYLTRFEFQRTEYWRTLYSSVGLGAPFLILCSKHDDLAPYQIVCNFSHRLQDLGADVKLLKWNNSLHAGHYRQYPTEYKAAVTELLEKAASVHLQKIQLEGERAGMEGTQDEISELICNLQKAAVNSNQSLRRVAIEPSDHFFLPSSAEYQNGRDSGPSPDELKERSVPVPDPPSISAHSVLGQFLFDVCVPKNIEGWDIKFSGSLNGQPLASARRYSQHVSKCTRRSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E2U3</accession>
    <name>A0AA39E2U3_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_003251</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate) + H2O = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:25017"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57836"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="EC" id="3.1.3.67"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PTEN phosphatase protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E2U3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016314">
        <property type="term" value="F:phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14509">
        <property type="entry name" value="PTP_PTEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000053">
        <property type="entry name" value="Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase TPTE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051281">
        <property type="entry name" value="Dual-spec_lipid-protein_phosph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055183">
        <property type="entry name" value="PTEN2A/B_C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045101">
        <property type="entry name" value="PTP_PTEN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014020">
        <property type="entry name" value="Tensin_C2-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029023">
        <property type="entry name" value="Tensin_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12305">
        <property type="entry name" value="PHOSPHATASE WITH HOMOLOGY TO TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12305:SF96">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3,4,5-TRISPHOSPHATE 3-PHOSPHATASE AND PROTEIN-TYROSINE-PHOSPHATASE PTEN2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22918">
        <property type="entry name" value="PTEN2_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22785">
        <property type="entry name" value="Tc-R-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01326">
        <property type="entry name" value="PTEN_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51182">
        <property type="entry name" value="C2_TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51181">
        <property type="entry name" value="PPASE_TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphatase tensin-type" evidence="8">
        <location>
            <begin position="135"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="7">
        <location>
            <begin position="249"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="C2 tensin-type" evidence="9">
        <location>
            <begin position="321"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="450"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="59"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="520"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="534"/>
            <end position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9705114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="607" mass="66364" checksum="8915B1FF94CEE7D9" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E325</accession>
    <name>A0AA39E325_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Nucleolar protein 14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_000005</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in nucleolar processing of pre-18S ribosomal RNA. Has a role in the nuclear export of 40S pre-ribosomal subunit to the cytoplasm.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NOP14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9707726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E325"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030692">
        <property type="term" value="C:Noc4p-Nop14p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007276">
        <property type="entry name" value="Nop14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23183">
        <property type="entry name" value="NOP14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23183:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04147">
        <property type="entry name" value="Nop14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="123"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="304"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="151"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="304"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="369"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="385"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="403"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="441"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="455"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9707726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="959" mass="110620" checksum="D15EB1343BFCBC10" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E379</accession>
    <name>A0AA39E379_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Exocyst complex component Sec6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_003491</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SEC6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E379"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051601">
        <property type="term" value="P:exocyst localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.50:FF:000003">
        <property type="entry name" value="Exocyst complex component SEC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.70:FF:000002">
        <property type="entry name" value="Exocyst complex component SEC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.70">
        <property type="entry name" value="Exocyst complex component Sec6, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010326">
        <property type="entry name" value="EXOC3/Sec6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042532">
        <property type="entry name" value="EXOC3/Sec6_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21292:SF1">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21292">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT SEC6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06046">
        <property type="entry name" value="Sec6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9705474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="747" mass="85241" checksum="FD2CE9B0422D13A3" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E4X3</accession>
    <name>A0AA39E4X3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Vacuolar iron transporter</fullName>
            <shortName evidence="9">VIT</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_000555</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Vacuolar Fe(2+) uptake transporter.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>Fe(2+)(in) = Fe(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:28486"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:28487"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Vacuole membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="9">The cytoplasmic metal binding domain (MBD) is located between transmembrane 2 (TM2) and transmembrane 3 (TM3).</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the CCC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E4X3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140315">
        <property type="term" value="F:iron ion sequestering activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005381">
        <property type="term" value="F:iron ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030026">
        <property type="term" value="P:intracellular manganese ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02435">
        <property type="entry name" value="CCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008217">
        <property type="entry name" value="Ccc1_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31851">
        <property type="entry name" value="FE(2+)/MN(2+) TRANSPORTER PCL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01988">
        <property type="entry name" value="VIT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0410">Iron transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0813">Transport</keyword>
    <keyword evidence="4 9" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="58"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="162"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="189"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="219"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU369115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9708578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="245" mass="26000" checksum="191E6E0743174BD3" modified="2024-01-24" version="1">MESMGGSGKQKLLLDDHKERHFTAGEIVRDVIIGVSDGLTVPFALAAGLSGANASSSIILTAGIAEVAAGAISMGLGGYLAAKSEADHYMRELKREQEEIITVPDIEAAEIEEILSQYGLEPHESAPVVNALRRRPQAWLDFMMKFELGLEKPDPRRAMQSAFTIALAYVLGGLVPLIPYMLVPRAEEAVIASVALTLVALLIFGYGKGRFTGNRPLRSALQTALIGALASAAAFGMAKAVRAVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E5A0</accession>
    <name>A0AA39E5A0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">threonine--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Threonyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_000827</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>tRNA(Thr) + L-threonine + ATP = L-threonyl-tRNA(Thr) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24624"/>
            <dbReference type="Rhea" id="RHEA-COMP:9670"/>
            <dbReference type="Rhea" id="RHEA-COMP:9704"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78534"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.3" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E5A0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004829">
        <property type="term" value="F:threonine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006435">
        <property type="term" value="P:threonyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01667">
        <property type="entry name" value="TGS_ThrRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00860">
        <property type="entry name" value="ThrRS_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00771">
        <property type="entry name" value="ThrRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000213">
        <property type="entry name" value="Probable threonine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000198">
        <property type="entry name" value="Threonine--tRNA ligase mitochondrial 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.800:FF:000019">
        <property type="entry name" value="Threonine--tRNA ligase mitochondrial 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000006">
        <property type="entry name" value="Threonine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.980.10:FF:000005">
        <property type="entry name" value="Threonyl-tRNA synthetase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.980.10">
        <property type="entry name" value="Threonyl-trna Synthetase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00184">
        <property type="entry name" value="Thr_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002320">
        <property type="entry name" value="Thr-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047246">
        <property type="entry name" value="ThrRS_anticodon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033728">
        <property type="entry name" value="ThrRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00418">
        <property type="entry name" value="thrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451:SF46">
        <property type="entry name" value="THREONINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451">
        <property type="entry name" value="THREONINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01047">
        <property type="entry name" value="TRNASYNTHTHR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="TGS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="ThrRS/AlaRS common domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TGS" evidence="13">
        <location>
            <begin position="50"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="12">
        <location>
            <begin position="322"/>
            <end position="584"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9709028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="686" mass="78436" checksum="117AF428E2DA6A50" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E8P7</accession>
    <name>A0AA39E8P7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">X8 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_000043</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9707782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E8P7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009506">
        <property type="term" value="C:plasmodesma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1040:FF:000001">
        <property type="entry name" value="Glucan endo-1,3-beta-glucosidase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012946">
        <property type="entry name" value="X8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044788">
        <property type="entry name" value="X8_dom_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31044">
        <property type="entry name" value="BETA-1,3 GLUCANASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31044:SF60">
        <property type="entry name" value="PLASMODESMATA CALLOSE-BINDING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07983">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00768">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041423310" description="X8 domain-containing protein" evidence="10">
        <location>
            <begin position="20"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="X8" evidence="11">
        <location>
            <begin position="21"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="109"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="109"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9707782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="197" mass="19925" checksum="6A7AA51999B91EA0" modified="2024-01-24" version="1">MAVLVYFVLFLAMTGHSSAAVYCVCKDGLGDQALQKTLDYACGAGADCSAILQNGACYNPNTVKDHCSYAVNSYFQKKGQASGSCDFAGTATQSQTNPSSVSTCVYPTSASSTGSSTTPSTTTPSTTPSTTPSTTTPSTTTPSTTTPSSSVFGLGPSGNGGITSTDSSAAVAPLHCTDLFFSAALTLWLSGLVLLWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39EAJ3</accession>
    <name>A0AA39EAJ3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="ORF">PVL29_000563</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="26">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="18">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="26">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39EAJ3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048544">
        <property type="term" value="P:recognition of pollen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00028">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00053">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000059">
        <property type="entry name" value="S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000621">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.10">
        <property type="entry name" value="Bulb-type lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000858">
        <property type="entry name" value="S_locus_glycoprot_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024171">
        <property type="entry name" value="SRK-like_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47974:SF6">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47974">
        <property type="entry name" value="OS07G0415500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00954">
        <property type="entry name" value="S_locus_glycop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000641">
        <property type="entry name" value="SRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 18" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="12 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7 21" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041370056" description="non-specific serine/threonine protein kinase" evidence="21">
        <location>
            <begin position="25"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="417"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="24">
        <location>
            <begin position="33"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="23">
        <location>
            <begin position="250"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="25">
        <location>
            <begin position="292"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="475"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="503"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EMBL" id="KAJ9708592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="789" mass="88893" checksum="17D19C5E0389C9C9" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YGP1</accession>
    <name>A0AA38YGP1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">PVL29_026580</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="25">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="25">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YGP1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000150">
        <property type="entry name" value="Putative LRR receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000015">
        <property type="entry name" value="Somatic embryogenesis receptor kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000016">
        <property type="entry name" value="Somatic embryogenesis receptor-like kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45974:SF266">
        <property type="entry name" value="LEUCINE-RICH REPEAT RECEPTOR PROTEIN KINASE HPCA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45974">
        <property type="entry name" value="RECEPTOR-LIKE PROTEIN 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="16 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10 23" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 22" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="23">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041414415" description="non-specific serine/threonine protein kinase" evidence="23">
        <location>
            <begin position="25"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="222"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="24">
        <location>
            <begin position="289"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="317"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="KAJ9670114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="612" mass="68841" checksum="C6220ADBF2EA9DC7" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YIY5</accession>
    <name>A0AA38YIY5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">UDP-glucose 4,6-dehydratase</fullName>
            <ecNumber evidence="12">4.2.1.76</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_025160</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>UDP-alpha-D-glucose = UDP-4-dehydro-6-deoxy-alpha-D-glucose + H2O</text>
            <dbReference type="Rhea" id="RHEA:21500"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:85329"/>
            <dbReference type="EC" id="4.2.1.76"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="8">Carbohydrate biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">In the C-terminal section; belongs to the dTDP-4-dehydrorhamnose reductase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">In the N-terminal section; belongs to the NAD(P)-dependent epimerase/dehydratase family. dTDP-glucose dehydratase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.76" evidence="12"/>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YIY5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008460">
        <property type="term" value="F:dTDP-glucose 4,6-dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050377">
        <property type="term" value="F:UDP-glucose 4,6-dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010280">
        <property type="term" value="F:UDP-L-rhamnose synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010253">
        <property type="term" value="P:UDP-rhamnose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05246">
        <property type="entry name" value="dTDP_GD_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05254">
        <property type="entry name" value="dTDP_HR_like_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000236">
        <property type="entry name" value="Bifunctional dTDP-4-dehydrorhamnose 3,5-epimerase/dTDP-4-dehydrorhamnose reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000304">
        <property type="entry name" value="UDP-glucose 4,6-dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005888">
        <property type="entry name" value="dTDP_Gluc_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029903">
        <property type="entry name" value="RmlD-like-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43000">
        <property type="entry name" value="DTDP-D-GLUCOSE 4,6-DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16363">
        <property type="entry name" value="GDP_Man_Dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04321">
        <property type="entry name" value="RmlD_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-binding" evidence="14">
        <location>
            <begin position="10"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="RmlD-like substrate binding" evidence="13">
        <location>
            <begin position="389"/>
            <end position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00050778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00061430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00061620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00067046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF16363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9671329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="675" mass="75762" checksum="E11E165D64F44710" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YJ03</accession>
    <name>A0AA38YJ03_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FRIGIDA-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_025136</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the Frigida family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YJ03"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009908">
        <property type="term" value="P:flower development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012474">
        <property type="entry name" value="Frigida"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31791">
        <property type="entry name" value="FRIGIDA-LIKE PROTEIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31791:SF49">
        <property type="entry name" value="INACTIVE PROTEIN FRIGIDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07899">
        <property type="entry name" value="Frigida"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3 5" id="KW-0221">Differentiation</keyword>
    <keyword evidence="4 5" id="KW-0287">Flowering</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="73"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="85"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9671301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="609" mass="66328" checksum="E01C80B8D4580620" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YKE2</accession>
    <name>A0AA38YKE2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_025632</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YKE2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45295">
        <property type="entry name" value="CHAPERONE PROTEIN DNAJ C76, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45295:SF4">
        <property type="entry name" value="OS06G0474800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="110"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="297"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9672063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="351" mass="39975" checksum="0EF864F3D6F674EE" modified="2024-01-24" version="1">MRDPELNNSQLLQAASEGTTLVVPRFASTSYVHVTDGYLSFPSLKDPRHSYHYKTRNSMWVSEVSLLHFSLPNSSQFDIPNNLTTRWRHKFTSVRCSSRSDGQSARTRKSYYELLGVSVDSKPQEIKEAYRKLQKKYHPDVAGQKGHEYSLMLNEAYRVLAREDLRREYDASIGKMTVGFGGIFSGMGYSSWKGPLRPQALFVDENECIGNTFVMDDALGCARVKVQYGDDDKKIEVSVDSCPVNCIHWVDREELAILEFLIQPQPKEGYGVFGGGWERPANVFMAAKAFNKQLKQQADHHQRNASRTVEEETPAQAEARANASMKIKKERLSRIWNWVQEFSGSNKANKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YKJ4</accession>
    <name>A0AA38YKJ4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">VOC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_025690</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YKJ4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004462">
        <property type="term" value="F:lactoylglutathione lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019243">
        <property type="term" value="P:methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="entry name" value="2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC_core"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46036">
        <property type="entry name" value="LACTOYLGLUTATHIONE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46036:SF5">
        <property type="entry name" value="LACTOYLGLUTATHIONE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="131"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9672150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="254" mass="29084" checksum="6FDC911A5C64B69A" modified="2024-01-24" version="1">MAETRKFAPGDDLLEWVQKDNRRFLHAVYRVGDIDRAIKFYIECFRMNVLRKRDFTKEKYSTASLGFDPKESHFVVELIYFCLFSYGVFVYKLTEVVRAYGGVATSEPACCRRGHHHLFGSSSRMASTPEPLCQIMLRVADLDFSIKFYEQYTMAMVGYGEMNETTVFELIYTYNVTEYTKGNGFVEVAVSTDDAYKSAAAVLLVSKEQGGKIIQPPGPIPVINAKMTSFVDPDDWKIVLIDNEDFLKQLQKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YMH7</accession>
    <name>A0AA38YMH7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Defective in cullin neddylation protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_026394</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Neddylation of cullins play an essential role in the regulation of SCF-type complexes activity.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9673094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YMH7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097602">
        <property type="term" value="F:cullin family protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031624">
        <property type="term" value="F:ubiquitin conjugating enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032182">
        <property type="term" value="F:ubiquitin-like protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045116">
        <property type="term" value="P:protein neddylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000269">
        <property type="entry name" value="Defective in cullin neddylation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.200:FF:000004">
        <property type="entry name" value="Defective in cullin neddylation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.200">
        <property type="entry name" value="Cullin, PONY binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014764">
        <property type="entry name" value="DCN-prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042460">
        <property type="entry name" value="DCN1-like_PONY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005176">
        <property type="entry name" value="PONY_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12281:SF2">
        <property type="entry name" value="DEFECTIVE IN CULLIN NEDDYLATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12281">
        <property type="entry name" value="RP42 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03556">
        <property type="entry name" value="Cullin_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51229">
        <property type="entry name" value="DCUN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DCUN1" evidence="2">
        <location>
            <begin position="34"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU410713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9673094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="239" mass="28021" checksum="CEB6D3756CD9AD14" modified="2024-01-24" version="1">MIEKVALHALKASDWHLEGAFDVFYSQPQIKAFTDSRHLEELYSRYKDQYVDMIMADGISVLCNDLQVDPQDIVMLVVSWHMKAATMCEFSKQEFIGGLQALGIDSLEKFRERIQFMRTELKDEQKFREIYNFAFGWAKEKGQKSLALDTAIGMWQLLFAEKQWALVDHWCQFLQARHNKAISRDTWSQLLEFAKTVDPSLSNYDAEGAWPYLIDEFVEYLNENNIIQKGQPIDWSQKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YMU1</accession>
    <name>A0AA38YMU1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Bromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_023100</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YMU1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04369">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051831">
        <property type="entry name" value="Bromodomain_contain_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22881">
        <property type="entry name" value="BROMODOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22881:SF11">
        <property type="entry name" value="BROMODOMAIN-CONTAINING PROTEIN DDB_G0270170-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bromo" evidence="4">
        <location>
            <begin position="185"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="272"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="486"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="614"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="656"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="20"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="30"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="63"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="93"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="156"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="285"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="311"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="621"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="672"/>
            <end position="690"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9673303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="696" mass="76093" checksum="BE5FCD85A3F6697F" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YRU5</accession>
    <name>A0AA38YRU5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">Laccase</fullName>
            <ecNumber evidence="4 13">1.10.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Benzenediol:oxygen oxidoreductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Diphenol oxidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Urishiol oxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_024335</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Lignin degradation and detoxification of lignin-derived products.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>4 hydroquinone + O2 = 4 benzosemiquinone + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:11276"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17594"/>
            <dbReference type="ChEBI" id="CHEBI:17977"/>
            <dbReference type="EC" id="1.10.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="13">Binds 4 Cu cations per monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Secreted</location>
            <location evidence="2 13">Extracellular space</location>
            <location evidence="2 13">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the multicopper oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.10.3.2" evidence="4 13"/>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YRU5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052716">
        <property type="term" value="F:hydroquinone:oxygen oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046274">
        <property type="term" value="P:lignin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13849">
        <property type="entry name" value="CuRO_1_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13875">
        <property type="entry name" value="CuRO_2_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13897">
        <property type="entry name" value="CuRO_3_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000049">
        <property type="entry name" value="Laccase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033138">
        <property type="entry name" value="Cu_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002355">
        <property type="entry name" value="Cu_oxidase_Cu_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034288">
        <property type="entry name" value="CuRO_1_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034285">
        <property type="entry name" value="CuRO_2_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034289">
        <property type="entry name" value="CuRO_3_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017761">
        <property type="entry name" value="Laccase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03389">
        <property type="entry name" value="laccase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF487">
        <property type="entry name" value="LACCASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00079">
        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00080">
        <property type="entry name" value="MULTICOPPER_OXIDASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0052">Apoplast</keyword>
    <keyword evidence="10 13" id="KW-0186">Copper</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 13" id="KW-0439">Lignin degradation</keyword>
    <keyword evidence="7 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 13" id="KW-0964">Secreted</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041485209" description="Laccase" evidence="13">
        <location>
            <begin position="25"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="16">
        <location>
            <begin position="36"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="14">
        <location>
            <begin position="161"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="15">
        <location>
            <begin position="421"/>
            <end position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9675344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="567" mass="63634" checksum="479DEF69B6758CCE" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YSC4</accession>
    <name>A0AA38YSC4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF4219 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_024633</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YSC4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35317:SF11">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35317">
        <property type="entry name" value="OS04G0629600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9675786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="111" mass="12698" checksum="A20D183C3E657EF0" modified="2024-01-24" version="1">MEESSLTAAPSILDGDNYETRVVRMTVHLQALDVWEAVEENYEVPPMGANPTVAQMKLHKERKTRKAKVKACLFATVSPSILIKIMKIDSAVEIWEYLKEEYKGDERIKNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YSR8</accession>
    <name>A0AA38YSR8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">PVL29_024808</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="24">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9676006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YSR8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905393">
        <property type="term" value="P:plant organ formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009791">
        <property type="term" value="P:post-embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048608">
        <property type="term" value="P:reproductive structure development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048367">
        <property type="term" value="P:shoot system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000511">
        <property type="entry name" value="Leucine-rich receptor-like protein kinase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000383">
        <property type="entry name" value="Leucine-rich repeat receptor protein kinase EMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000632">
        <property type="entry name" value="leucine-rich repeat receptor-like protein kinase TDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000234">
        <property type="entry name" value="Probable inactive receptor kinase RLK902"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000215">
        <property type="entry name" value="Receptor-like protein kinase HSL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051716">
        <property type="entry name" value="Plant_RL_S/T_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48053">
        <property type="entry name" value="LEUCINE RICH REPEAT FAMILY PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48053:SF159">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="19" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="17 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="13 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="11 22" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="10 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041314096" description="Protein kinase domain-containing protein" evidence="22">
        <location>
            <begin position="28"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="615"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="679"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="708"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KAJ9676006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="974" mass="107686" checksum="D06ABE331F0E7515" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YT36</accession>
    <name>A0AA38YT36_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SnoaL-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_024937</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9676186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YT36"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037401">
        <property type="entry name" value="SnoaL-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33698">
        <property type="entry name" value="NUCLEAR TRANSPORT FACTOR 2 (NTF2)-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33698:SF3">
        <property type="entry name" value="OS09G0266000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12680">
        <property type="entry name" value="SnoaL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SnoaL-like" evidence="2">
        <location>
            <begin position="84"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9676186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="225" mass="25163" checksum="FB0F4B2372F80DDA" modified="2024-01-24" version="1">MSLLTNSTTLLRPSKLSPPLLHPHTASIATRSTQRRSRRCNPLRVSSSPQNPTATIAEPVTTAQSDEANDDGDEFKLLSGSSVVRAFYTGINSHDLGSVEELIAHNCVYEDLIFPQPFVGRKAIMDFFNKFIDTISMELQFVIDDISEADSSAVGVTWHLEWKGRPFPFSKGCSFYRLEVLNGKRQIIYGRDSVEPFFKPGETALVAIRGVAWLLQQFPKLADWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YT48</accession>
    <name>A0AA38YT48_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C-JID domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_024951</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9676203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YT48"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045344">
        <property type="entry name" value="C-JID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011713">
        <property type="entry name" value="Leu-rich_rpt_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017:SF479">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN (TIR-NBS-LRR CLASS) FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20160">
        <property type="entry name" value="C-JID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07725">
        <property type="entry name" value="LRR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="C-JID" evidence="3">
        <location>
            <begin position="367"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9676203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="577" mass="65223" checksum="487BB0C9CB00EA18" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YT89</accession>
    <name>A0AA38YT89_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_024995</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9676260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YT89"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010333">
        <property type="term" value="F:terpene synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016102">
        <property type="term" value="P:diterpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00684">
        <property type="entry name" value="Terpene_cyclase_plant_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.600.10:FF:000007">
        <property type="entry name" value="Isoprene synthase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.130:FF:000001">
        <property type="entry name" value="Isoprene synthase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.130">
        <property type="entry name" value="Terpene synthase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034741">
        <property type="entry name" value="Terpene_cyclase-like_1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044814">
        <property type="entry name" value="Terpene_cyclase_plant_C1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001906">
        <property type="entry name" value="Terpene_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036965">
        <property type="entry name" value="Terpene_synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050148">
        <property type="entry name" value="Terpene_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005630">
        <property type="entry name" value="Terpene_synthase_metal-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225">
        <property type="entry name" value="OS04G0344100 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225:SF241">
        <property type="entry name" value="TERPENE SYNTHASE FAMILY, METAL-BINDING DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01397">
        <property type="entry name" value="Terpene_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03936">
        <property type="entry name" value="Terpene_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01019">
        <property type="entry name" value="Terpene_Cyclase_Like_1_C_Termi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Terpene synthase N-terminal" evidence="5">
        <location>
            <begin position="46"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="Terpene synthase metal-binding" evidence="6">
        <location>
            <begin position="278"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9676260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="553" mass="64498" checksum="11E77E1DBF40AF2A" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YUU8</accession>
    <name>A0AA38YUU8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Inositol polyphosphate multikinase</fullName>
            <ecNumber evidence="8">2.7.1.140</ecNumber>
            <ecNumber evidence="8">2.7.1.151</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_022098</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Inositol phosphate kinase with a broad substrate specificity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>1D-myo-inositol 1,3,4,6-tetrakisphosphate + ATP = 1D-myo-inositol 1,3,4,5,6-pentakisphosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12717"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57660"/>
            <dbReference type="ChEBI" id="CHEBI:57733"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.140"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 8">
            <text>1D-myo-inositol 1,4,5-trisphosphate + 2 ATP = 1D-myo-inositol 1,3,4,5,6-pentakisphosphate + 2 ADP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:32359"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57733"/>
            <dbReference type="ChEBI" id="CHEBI:203600"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.151"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the inositol phosphokinase (IPK) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.140" evidence="8"/>
    <dbReference type="EC" id="2.7.1.151" evidence="8"/>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9676932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YUU8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051765">
        <property type="term" value="F:inositol tetrakisphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008440">
        <property type="term" value="F:inositol-1,4,5-trisphosphate 3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032958">
        <property type="term" value="P:inositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.160">
        <property type="entry name" value="Inositol polyphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005522">
        <property type="entry name" value="IPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038286">
        <property type="entry name" value="IPK_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12400">
        <property type="entry name" value="INOSITOL POLYPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12400:SF51">
        <property type="entry name" value="INOSITOL POLYPHOSPHATE MULTIKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03770">
        <property type="entry name" value="IPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00036164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00036525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9676932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="305" mass="34195" checksum="BF78D52F29D75E1C" modified="2024-01-24" version="1">MLKVPDHQVAGHHARDGKLGPLIDDSGRFYKPLQGDERGSKEVAFYTSFSSNARIPDHIRRFFPIFYGTQLLEASDGSGLYPHLVLQDVVSGHHNPCIIDVKIGSRTWYLQASEDYIQRSLKKDRETTSLSLGFRISGLQIYDSEESGFWRPEKKRVLGFTADDVRVVLRKFVSSNSPTDSDSEPDCSFASAVYGGSTGILAQLLELKAWFEDQTIFHFFSCSILMMYDKEAIFKGMSSGAEIKLIDFAHVVEGEGVIDHNFLGGLCSLIKMISEILTSPDENPNKTCLQDYEKNHSYLENGTTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YVF8</accession>
    <name>A0AA38YVF8_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_022425</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YVF8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33474">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33474:SF2">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9677430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="53" mass="6130" checksum="89CAE840435E63FE" modified="2024-01-24" version="1">MHGYHQGHQVSEVSFLNNMEKIWEEQNIRGRTDVELNDYPGSGANNRHTPTPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YW08</accession>
    <name>A0AA38YW08_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_022563</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YW08"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009736">
        <property type="term" value="P:cytokinin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009740">
        <property type="term" value="P:gibberellic acid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010090">
        <property type="term" value="P:trichome morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044299">
        <property type="entry name" value="GIS3/ZFP5/ZFP6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46353">
        <property type="entry name" value="ZINC FINGER PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46353:SF5">
        <property type="entry name" value="ZINC FINGER PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="86"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="171"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="174"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9677645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="196" mass="22087" checksum="57C8AC4EDF645DD6" modified="2024-01-24" version="1">MENHVSQQNLAATNNPFLPGGVGDGSFGSKIHGENKKLRLFGFEVDLYPKDAELQGGSELDETANSPDVALSKMEKNVEKPEHKKYECQFCLKKFANSQALGGHQNAHKKERLEKKRLQLQGRKGSLNFYFQPLQSDVGFIYHESPWFYDSVSSSIPEFMLYKEPHISFDEPQASGSTLSQPIPTRHPFAKPETEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YX13</accession>
    <name>A0AA38YX13_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_023006</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9678271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YX13"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004866">
        <property type="term" value="F:endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23375">
        <property type="entry name" value="beta-trefoil_STI_VvMLP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011065">
        <property type="entry name" value="Kunitz_inhibitor_STI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002160">
        <property type="entry name" value="Prot_inh_Kunz-lg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33107">
        <property type="entry name" value="KUNITZ TRYPSIN INHIBITOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33107:SF5">
        <property type="entry name" value="KUNITZ TRYPSIN INHIBITOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00197">
        <property type="entry name" value="Kunitz_legume"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00291">
        <property type="entry name" value="KUNITZINHBTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00452">
        <property type="entry name" value="STI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50386">
        <property type="entry name" value="STI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00283">
        <property type="entry name" value="SOYBEAN_KUNITZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041429862" evidence="1">
        <location>
            <begin position="28"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9678271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="205" mass="22550" checksum="46D8C82A41BEE165" modified="2024-01-24" version="1">MKTSSLLLFSLLLIALAFNPLPGAAEAAPDPVLDIEGKQLRSGVDYYILPVIRGRGGGLTVASVRNKTCPLDVVQDKLEVSHGLPLTFTPVNPKQDVIRVSTDHNIKFSAATICAQSTVWKTEYDESTGQRFITTGGVEGNPGPGTLSNWFKIEKYEDDYKLVFCPTVCNFCKVICRDVGVYIQNGYRRLALTTDAPFRVMFKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YX47</accession>
    <name>A0AA38YX47_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_022903</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9678152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YX47"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9678152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="142" mass="15889" checksum="0913A464DA42BE17" modified="2024-01-24" version="1">MWPHYTHPSCYPCPFLTHTYEDHTLHDAPVDISENFQQVQRWHMAGNSINGISSSSIDFLNRFNASMNGVIDTRNEKPSASGDLSFFQFETTGNEGLFGDRPLCKWVGRGESADLQVGGSSLNPVEDEDDPSPLLDQWAELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YXX7</accession>
    <name>A0AA38YXX7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_020817</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the AP2/ERF transcription factor family. ERF subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YXX7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009873">
        <property type="term" value="P:ethylene-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000001">
        <property type="entry name" value="Ethylene-responsive transcription factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044808">
        <property type="entry name" value="ERF_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31190">
        <property type="entry name" value="DNA-BINDING DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31190:SF499">
        <property type="entry name" value="ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR ERF105"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00367">
        <property type="entry name" value="ETHRSPELEMNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0936">Ethylene signaling pathway</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="10">
        <location>
            <begin position="115"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="79"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="91"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9678741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="274" mass="30729" checksum="25758D2FF488EF4D" modified="2024-01-24" version="1">MAEQASSLHFIHQQLLSDFESFEKLNDPSQTSTSDSSVSTSNIIPLSNYFDLHEDENNPFLLHCSTSAPSGFFQFESKSPKASRLSHRRPPLSISLPPPTISQSPADSDSGEVRRYRGVRRRPWGKFAAEIRDPNRRGSRVWLGTFETAIEAARAYDRAAFKMRGSKAILNFPLEAGNWSGSDPLAISGQKRVRESEGEEREQVEIKVLKQEEQSPESEITVAAAATNVFGVNPLTPSNWRAVWEEREMEGIFKLPPLTPFSPHPWLGYSQLIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YYQ1</accession>
    <name>A0AA38YYQ1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Exostosin GT47 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_021158</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 47 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YYQ1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008378">
        <property type="term" value="F:galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009969">
        <property type="term" value="P:xyloglucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004263">
        <property type="entry name" value="Exostosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040911">
        <property type="entry name" value="Exostosin_GT47"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11062">
        <property type="entry name" value="EXOSTOSIN HEPARAN SULFATE GLYCOSYLTRANSFERASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11062:SF214">
        <property type="entry name" value="XYLOGLUCAN GALACTOSYLTRANSFERASE XLT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03016">
        <property type="entry name" value="Exostosin_GT47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="Exostosin GT47" evidence="7">
        <location>
            <begin position="92"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9679146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="489" mass="56726" checksum="34DCA17EE729D65E" modified="2024-01-24" version="1">MLPVSDNPSTELHKKPKTPLFFDRKSSFSSLQAYFSVYHPRTWLIISILLLQVLLLFMARSLPISISIPRNRQHFRTPFTAPAVQVSGDPECAFGKVYVYDLPPFFNRELVKNCDKLNPWSSRCDTLTNDGFGQRATGLAGVVPEDLMPAWYWTDQFVTEIIFHNRILKHPCRTFEPDSATAYYIPFYAGLAVGKYLWSNCSPQDRDRHGEMLLTWVRDQPYWNRSNGWDHFLTLGRITWDFRRSKDGDWGSSLIYMPLMRNITRLLIERNPWDYFDVGVPYPTGFHPRSDADVLQWQHHVRTRNRTTLFSFAGATRGAIRNDFRGLLLSHCLNESDSCRVVNCAGTRCSNGTSAILKSFLDSDFCLQPRGDSFTRRSIFDCMIAGSIPVFFWRRTAYFQYEWFLPSEPGSYSVFIHRNEVKNGTSIRGVLESYSREEVRKMREKVIDYIPKLVYARPDAGLESFKDAFDVAIDGVLRRMKEQEGGYKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z2C2</accession>
    <name>A0AA38Z2C2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_019873</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z2C2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47068">
        <property type="entry name" value="OS02G0659100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47068:SF1">
        <property type="entry name" value="OS02G0659100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="239"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="321"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="56"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="130"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="416"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="63"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="417"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9680684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="491" mass="53876" checksum="1292E68A14F1C1E6" modified="2024-01-24" version="1">MEGRERLTPQSMTAPMQHHPPLFVQYLTRRRHYQPPPICKKGFGCGRALGGHMRAHGIGDESGNIEEEDPASDWEDKLGGHVPPNTKKMYALRTNPNRLKSCCRVCENCGKEFLSWKSFLEHGKCNSDDAESLVSSPGSDGEDGSGRKGYGWSKRKRSLRAKVGNFISNCPSSEEEDLAHCLVMLSNGTVDPILVAEPEESCASASKEEERRNPIGFAAPMSCRLPLEKAKGVVGKGMFECKACKKVFNSHQALGGHRASHKKVKGCFAARLDHMDDSLADEDVITHDEFSVPSKSTSTLQFEHASNAALSFPSKRKSKVHECSICHRVFSSGQALGGHKRCHWITSTAPDTSSLSKFHHFHDHLEQIQQRPKLPKTTPLDLNLPAPVDDIAGIRRDPRNPLSFEVSTDIYLQTFPNNHNHQNDAGGNNNDNDVNNIDDSNGLIHSNVEDEADSKVKLAKLSDLKDINMSGGSSPWLQVGIGSATNVGADS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z5U2</accession>
    <name>A0AA38Z5U2_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_018789</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z5U2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36748">
        <property type="entry name" value="MENTAL RETARDATION GTPASE ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36748:SF3">
        <property type="entry name" value="MENTAL RETARDATION GTPASE ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="146"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="183"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="191"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="231"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9682935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="301" mass="34278" checksum="7B6C3E40ABCCDB9B" modified="2024-01-24" version="1">MEQSRGDIEPEFNLREWARKARISRENTISRRFSASNIRREDTRSFRSSITISSTASSPGYTLKDEIDPATYSFTSALKALQARSGYGWECSSPDGFALNSKWNEAEKYICNPLSGEVPMECLSAKTLSGRSFRNFTNRITMSAPLIYPSQPRQPQTNYSAAAPTTQENFVQFPIQEKKMEGMTRDVGTQSTPPDLSSSSPSPTSTPSIIERSLQRCGAEGGESPNSNAKLKSEEEVEVKDTREKEETKRKEEEQIKKEKQMCRCRQGGCLSWRSLWMRKRHREKHKPRKKNIFLQHIKGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z691</accession>
    <name>A0AA38Z691_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein DETOXIFICATION</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_018689</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z691"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13132">
        <property type="entry name" value="MATE_eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045069">
        <property type="entry name" value="MATE_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="48"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="94"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="136"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="166"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="193"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="232"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="274"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="315"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="354"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="388"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="418"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9682813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="442" mass="47678" checksum="72D1CD33330E1537" modified="2024-01-24" version="1">MEMGGNMGVFRKEMKKTSLLAAPMVVTTLLQYLMQVVSLMMVGHLGRLPLSAVAIATALTNVSGFSLLSGMAGGLETLCGQAYGAQQYHKLGNYTYSAIISLALVCVPISVLWIFMDKLLILIGQDPLISLEARKYSVWLIPGLFGCAVLKPTVRYLQTQSLILPLLISSFIVLCFHVPVCWILIFKLELGDIGAAVAFCLSNWYNVILLGLYVKYSSACEATRMKFSKETFLVIVEFFRFAVPAAVMVCLKWWSCELITLLSGLLPNPKLESSVLAICLTITTLHFTIPYGLGAVASTRVSNELGAGNSQAAQIAVWPVILLAVIDAVTVSTVLFSCRYVLGEAYSNDKQVVGYVAVMTPLICISIMMDSLQGVLSGVPRGSGRQKIGAYVNLGAFYLVGVPVAVILGFVLRLKGKGLWIGIVAGSVVQATLLFLITGFTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z6L4</accession>
    <name>A0AA38Z6L4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein FLX-like 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_018817</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FLX family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z6L4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009908">
        <property type="term" value="P:flower development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040353">
        <property type="entry name" value="FLX/FLX-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33405">
        <property type="entry name" value="PROTEIN FLX-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33405:SF4">
        <property type="entry name" value="PROTEIN FLX-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0221">Differentiation</keyword>
    <keyword evidence="5" id="KW-0287">Flowering</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="228"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="60"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="276"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="302"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9682978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="424" mass="45487" checksum="EA598C1D933C5D1F" modified="2024-01-24" version="1">MGSKGRIPPHLRRPLPGPGLMHPDPFGPGIRPPHGVFPPFDMLPPPEIMEQKLAAQHVDMQRLANENQRLAATHGTLRQELAAAQHELQMLQAQIGVVKSEREQRMRGLTDKIAKMEAELKASEPVKLELQQARADAQSLVAARQELISKVQQLTQDLQRSHSDVQQIPALMAELESLRQEYQHCRATYDYEKKLYNDHLESLQVMEKNYMTMAREVEKLRAELTNTANMDRRTGGPYGGPTGYNEGEASGHHPIGHNAYEDGYGVPQGRAPLPGATGGGVAAAGGGTPAYAGPQSGPAASRPGYDGPRGPGYDAPRGPGYDAPRGPSYDAQRGPGYDAQKGPGHDAQRGPGYDAQRGPAYDGQRGPVYDAPRGTNYDAPTRAAPGPHGQVPSGNNVPYGSATPPARSGGGYEAAPRGGNPVRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z7U1</accession>
    <name>A0AA38Z7U1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNase H type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_019178</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z7U1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09279">
        <property type="entry name" value="RNase_HI_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000076">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.970.10">
        <property type="entry name" value="Ribonuclease H1, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009027">
        <property type="entry name" value="Ribosomal_bL9/RNase_H1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011320">
        <property type="entry name" value="RNase_H1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037056">
        <property type="entry name" value="RNase_H1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002156">
        <property type="entry name" value="RNaseH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46387">
        <property type="entry name" value="POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46387:SF2">
        <property type="entry name" value="RIBONUCLEASE HI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01693">
        <property type="entry name" value="Cauli_VI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13456">
        <property type="entry name" value="RVT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55658">
        <property type="entry name" value="L9 N-domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50879">
        <property type="entry name" value="RNASE_H_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase H type-1" evidence="1">
        <location>
            <begin position="130"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9683489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="273" mass="30041" checksum="D1FF0FD617332C36" modified="2024-01-24" version="1">MGLCYGAVSGLQSWFWIIMEEEKDAFYVVRKGDIVGLYKSFSECQAQAGFSVCDPSVSVYKGYCLPKDAEEFLASHGLKNASYVINAADVKGDIFGKLQACPFQQPGSSKGTSNQDLPQKRLHKAIEYINFHSWILQFDGASKGNPGQAGAGAVLRAVDGSAVIHLREGVGIATNNVAEYRALILGMKYALKKGIKRICARGDSQLVCMQFQGLWKTKNQNMANLCEEAKELGKKFSSFQIEHVLREFNSEADAQANLAVNLMDGQVQEEYKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z7W1</accession>
    <name>A0AA38Z7W1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_016507</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z7W1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051177">
        <property type="entry name" value="CIK-Related_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12984:SF3">
        <property type="entry name" value="N-TERMINAL KINASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12984">
        <property type="entry name" value="SCY1-RELATED S/T PROTEIN KINASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="2"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="1">
        <location>
            <begin position="523"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="595"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="636"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="662"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="686"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="599"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="701"/>
            <end position="710"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9684048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="794" mass="85971" checksum="81DC2F60F6020AC8" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z839</accession>
    <name>A0AA38Z839_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_016314</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9683758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z839"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF78">
        <property type="entry name" value="OS12G0587133 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9683758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="678" mass="78245" checksum="2B2170DA6E976586" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z888</accession>
    <name>A0AA38Z888_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_016623</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z888"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045884">
        <property type="entry name" value="At5g59350-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34054">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34054:SF2">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="136"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="285"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="144"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="289"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9684225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="320" mass="36021" checksum="AF760609FDB6B9DB" modified="2024-01-24" version="1">MRSLSSVGLGLSIVFGCLLLALVAELYYLLWWKKRLTSTEMEDDYSSPARELLYMYCWKKPSSLSSRALNPQELCSLDRVTDTHALEPQQAHHQLHMHGNKDYLLKPFGEETMETELMRLHDLSGPPRFLFTIKEETKEDLESEDGKSRGDKSAKGSRSRSLSDLLTVETPFLTPLASPPFFTPPLTPMDSSYNHHGVNVLFESATDADQFSKISSSPPPKFKFLKDAEEKLHRKVMEEAKKEQLQFEVSADNAVNSPSSSKLHKDEEDGSFITIVVAKNKEKDLTHHSSSSQILPLVSSPSTFDPPASKKPILHHSFTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z9X6</accession>
    <name>A0AA38Z9X6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Ribosomal RNA small subunit methyltransferase NEP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_017240</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase NEP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z9X6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070037">
        <property type="term" value="F:rRNA (pseudouridine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18088">
        <property type="entry name" value="Nep1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1280.10:FF:000003">
        <property type="entry name" value="Ribosomal RNA small subunit methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005304">
        <property type="entry name" value="Rbsml_bgen_MeTrfase_EMG1/NEP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12636">
        <property type="entry name" value="NEP1/MRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12636:SF5">
        <property type="entry name" value="RIBOSOMAL RNA SMALL SUBUNIT METHYLTRANSFERASE NEP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03587">
        <property type="entry name" value="EMG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="8" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="19"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9685128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="271" mass="30713" checksum="01EB2AEF155B8ADF" modified="2024-01-24" version="1">MVRPYAIKGHKRKKREENYDREEVEQLLEEETAATGTQDEKKAEEVKLELPGIPLVPSNLNNKTGVTFILERACLEVAKVGKSYQLLSSEDHANFLRKNNKNPAEYRPDILHQALLTILDSPLNKAGRLRAVYVRTEKGVLFEVKPHVRIPRTYKRFSGIMLQLLQKLSIAAVGKREKLLRVIKNPVTQYLPVNSRKIGFSYSSEKSIPMRDYVAAVSDDVDLVFVVGAMAHGKISNDYTDDFIAISGYPLSAAWCTYLICGALMQKWNIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z9Z5</accession>
    <name>A0AA38Z9Z5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Disease resistance RPP13-like protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_017264</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14798">
        <property type="entry name" value="RX-CC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056789">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038005">
        <property type="entry name" value="RX-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766:SF51">
        <property type="entry name" value="DISEASE RESISTANCE RPP13-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766">
        <property type="entry name" value="PLANT BROAD-SPECTRUM MILDEW RESISTANCE PROTEIN RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25019">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0611">Plant defense</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="8">
        <location>
            <begin position="9"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="7">
        <location>
            <begin position="189"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="9">
        <location>
            <begin position="442"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="domain" description="R13L1/DRL21-like LRR repeat region" evidence="10">
        <location>
            <begin position="706"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="126"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF25019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9685158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1411" mass="160182" checksum="73E5F72275FA9D8E" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZAQ2</accession>
    <name>A0AA38ZAQ2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF8040 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_017543</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZAQ2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058353">
        <property type="entry name" value="DUF8040"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045249">
        <property type="entry name" value="HARBI1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930:SF268">
        <property type="entry name" value="NUCLEASE HARBI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26138">
        <property type="entry name" value="DUF8040"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF8040" evidence="1">
        <location>
            <begin position="32"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF26138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9685546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="157" mass="18608" checksum="E635D628FFB3CACF" modified="2024-01-24" version="1">MYATYATILVSVYHCSNYLERSISNEGDYERHALMERLTLMDDEDCYNQLRMRKDAFTRLVNILWGTGCLRNSAYSNVEEQVAKFLHIVSHNLRNRIMKFHFKRSSETISCHFHQVLRAIISLDDAFLKQPDGLKCPQEIKDNTKFWPYFNLSNKHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZCG7</accession>
    <name>A0AA38ZCG7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_015307</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZCG7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001778">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45717:SF10">
        <property type="entry name" value="OS10G0501000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45717">
        <property type="entry name" value="OS12G0527900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="135"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="345"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9686344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="499" mass="57168" checksum="345C42C4AB1B9303" modified="2024-01-24" version="1">MQIIRRCAVNTSSISRVVRVLPYSTETLTSNAPKESLQSRISPAIDLRVSIVPALEQWRKEGQSIKHQDLHRLIRKLRTFKRYNHALEIYEWIRDKFYFDISPGDVAIQLDLISKVHGLEQAEKYFNETPNSLRSFQVYGALLNCYSQKKSLEKAEAIMQEMRDMGFVKTLSYNVMLGLYSRLEKHEKLDNLMQEMEENGIGLDSFTYCIRLNAYGATSDMEGMEKLLMKLETDPAVKSDWNAYIVAANGYLKADLKEKAVEMLKKSEQFISGRSRRFGYEILLTLYATMGNKTEVYRIWNLYKTIGKFFNTGYVAMVSSLLKLDDMDGAEKTFEEWLSGNKFFDFRVPNLLIRAYCKKGLLEKAEQLVSRAIEQGEKPTAVTWNALAAGYHENNQMEKAVDTLKKALLATLQGWKPNPVTLSACLEYLKGKGDVEEAENLVRLLREQSLVSACDFDRLVDYIRSEEPGSNTIAQMLWDHEDLDAETDGVVNLKQESIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZEX1</accession>
    <name>A0AA38ZEX1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_016170</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily. AB hydrolase 4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZEX1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034338">
        <property type="term" value="F:short-chain carboxylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000071">
        <property type="entry name" value="Embryogenesis-associated protein EMB8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050960">
        <property type="entry name" value="AB_hydrolase_4_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10794">
        <property type="entry name" value="ABHYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10794:SF82">
        <property type="entry name" value="ALPHA_BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="518"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="4">
        <location>
            <begin position="150"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="476"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9687575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="556" mass="62312" checksum="AC01AF93D6A9F856" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZHM0</accession>
    <name>A0AA38ZHM0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DYW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_014286</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. PCMP-H subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZHM0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031425">
        <property type="term" value="P:chloroplast RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001050">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At2g33760"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000231">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000031">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000309">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000576">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032867">
        <property type="entry name" value="DYW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046849">
        <property type="entry name" value="E2_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF436">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN ELI1, CHLOROPLASTIC-LIKE ISOFORM X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14432">
        <property type="entry name" value="DYW_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20430">
        <property type="entry name" value="Eplus_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="104"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="202"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="303"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="373"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="404"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="domain" description="DYW" evidence="5">
        <location>
            <begin position="619"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="19"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF14432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9688558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="711" mass="79605" checksum="C684D06824863456" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZJ88</accession>
    <name>A0AA38ZJ88_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Metaxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_014774</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Involved in transport of proteins into the mitochondrion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the metaxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9689264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZJ88"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001401">
        <property type="term" value="C:SAM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006626">
        <property type="term" value="P:protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03193">
        <property type="entry name" value="GST_C_Metaxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017410">
        <property type="entry name" value="Metaxin1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033468">
        <property type="entry name" value="Metaxin_GST"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050931">
        <property type="entry name" value="Mito_Protein_Transport_Metaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012336">
        <property type="entry name" value="Thioredoxin-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12289:SF41">
        <property type="entry name" value="FAILED AXON CONNECTIONS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12289">
        <property type="entry name" value="METAXIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17171">
        <property type="entry name" value="GST_C_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17172">
        <property type="entry name" value="GST_N_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038150">
        <property type="entry name" value="Metaxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="290"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin-like fold" evidence="6">
        <location>
            <begin position="25"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Metaxin glutathione S-transferase" evidence="5">
        <location>
            <begin position="172"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="242"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="242"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR038150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF17171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF17172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9689264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="326" mass="36806" checksum="2C30BF6F587A683C" modified="2024-01-24" version="1">MDELDNQKLTLVARKPCFGLPTACPSCLPVYIYLRFAQVPFDLSFNLIHPDSDQIPYIESGAYVAYNNEKGGVLESLKEDGIIDLDTQLHAIPEWLSTKVMISTWLEEAIMYELWVCSDGSSAKKIYYSDLPWPIGKILYLKQIHAVKQLLGITKDNAERREEEIHRRATIAYGALSTRLGEQTFFFENRPSGVDALFLGHALFTLQALPDTSVLRSKLLEHSNLVRYAEKLKAEFMEAGSSSSSVPQFPAEPSSSTSRRGPSNWSSKAKSKPKREKTEEEKTFRRRAKYFLVTQLVAVLVFLSLLGVSGGDNEVDLDDEDDDYNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZL48</accession>
    <name>A0AA38ZL48_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_013324</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZL48"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9691101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="133" mass="15301" checksum="27C5D06112628809" modified="2024-01-24" version="1">MRWSPENQCGKIAALILFRIGFFDKLLNCDQLLQHPFISSSSPNIIFSKADLKSAKERRSCRVEKSRVELEELEGISVESANAVWFQREKRLNCCCLKKYKARNLDSLIRSVGESALTMEILVVNKFRTFVVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZM99</accession>
    <name>A0AA38ZM99_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_013689</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZM99"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000125">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000344">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF473">
        <property type="entry name" value="(WILD MALAYSIAN BANANA) HYPOTHETICAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="75"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="106"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="257"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9691577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="354" mass="39469" checksum="0F8A780EC424F5CA" modified="2024-01-24" version="1">MLQSGLKPDHMTYPFVIKACNESSMTWFGLLVHTHVVKSGFECDSYIVSSLIHLYANGKDLGAAKQLFNLCSDRDVVSWNAMIDGYVKPGEMGHARMVFDRMVWRDVISWNTMVNDYAIVGKIDEAKRLFDEMPERNLVSWNSMLAGFVKCGNVEDAFGLFSEMPCRDVCGKPNEALTLFDQMRAVGVKPTEATVVSLLSACAHLGSLDKGLHLHNYINDNRIEVNSIVGTALVDMYAKCGKISLATQVFNAMESKDVLAWNTIIAGMAIHGHVQEAQQLFKEMKEAGVEPNEPYSRESQLKYHDPSLSSKRLISKKTPLKIGLGISPYCRLSSILLYIHTHIQQSSLSLQIAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZMB1</accession>
    <name>A0AA38ZMB1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_013603</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZMB1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF78">
        <property type="entry name" value="OS12G0587133 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="398"/>
            <end position="652"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9691474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1104" mass="126817" checksum="887BF61D5DA3114A" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZP61</accession>
    <name>A0AA38ZP61_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_011682</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZP61"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10747">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10719">
        <property type="entry name" value="DnaJ_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000037">
        <property type="entry name" value="Chaperone protein dnaJ A6 chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.230.10:FF:000006">
        <property type="entry name" value="Chaperone protein dnaJ A6 chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.20:FF:000025">
        <property type="entry name" value="Molecular chaperone Hsp40/DnaJ family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.20:FF:000009">
        <property type="entry name" value="Putative Mitochondrial DnaJ chaperone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.230.10">
        <property type="entry name" value="Heat shock protein DnaJ, cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.260.20">
        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01152">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012724">
        <property type="entry name" value="DnaJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001305">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02349">
        <property type="entry name" value="DnaJ_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008035">
        <property type="entry name" value="PRK10767.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43096">
        <property type="entry name" value="DNAJ HOMOLOG 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43096:SF22">
        <property type="entry name" value="MOLECULAR CHAPERONE HSP40_DNAJ FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00684">
        <property type="entry name" value="DnaJ_CXXCXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="DnaJ/Hsp40 cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="HSP40/DnaJ peptide-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51188">
        <property type="entry name" value="ZF_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="J" evidence="7">
        <location>
            <begin position="71"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="CR-type" evidence="8">
        <location>
            <begin position="205"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CR-type" evidence="5">
        <location>
            <begin position="205"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="431"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="448"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9692715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="486" mass="52608" checksum="0686893E8A9A3300" modified="2024-01-24" version="1">MAITSLSSLSFNPDRPLLLFSSSNGPSSSSSSSFFTGGTHFRTHKSLACVNLPSSSLSSKRLSVVVAASSDYYSTLGVPKSASSKEIKAAYRKLARQYHPDVNKQPGATEKFKEISAAYEVLSDDKKRALYDQFGEAGVKSGVGGQAGAYTTNPFDLFETFFGPSMGGFSGMDQAGFRTRRRTTVSKGEDIRYDMTLKFSEAIFGAEKEFELSHLETCEVCTGTGAKIGSKMRICSTCGGRGQVMRTEQTPFGLFSQVSVCPTCGGDGEVISEYCRKCSGEGRIRVKKDIKVKVPPGVSKGSILRVAGEGDAGLRGGPPGDLFVYLDVEEIPEIQRDGINLCSTVTVSYLDAILGAVVKVKTVEGVTELQIPSGTQPGDVLVLAKKGAPKLNRPTIRGDHLFTVKVTIPNRISAKERELLEELAFLRNNTSSWSRTRPKAQPATKSMESQKDAVTEKNEDAGDDNDVWKKLKDFAGTWQSKLKWLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZRT2</accession>
    <name>A0AA38ZRT2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_009671</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9693814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZRT2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041331431" description="Secreted protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9693814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="102" mass="11189" checksum="4B3B28C273D3AF75" modified="2024-01-24" version="1">MVNFSCLFFLANSIGVDAWNFSSDLVLQARCRGLKRADHVAFPLKGSNMEDEANGSASAGLSYRIEIKDAYIPPWIICSICATMGSEGRSFEARYVKSCHCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZW28</accession>
    <name>A0AA38ZW28_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PGG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_008383</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZW28"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000978">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026961">
        <property type="entry name" value="PGG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24177">
        <property type="entry name" value="CASKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24177:SF103">
        <property type="entry name" value="PGG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13962">
        <property type="entry name" value="PGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="677"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="721"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="749"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="domain" description="PGG" evidence="3">
        <location>
            <begin position="629"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="280"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="393"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="280"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="313"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="422"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="461"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9696109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="794" mass="89291" checksum="78DEF7C2C6243917" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZWZ1</accession>
    <name>A0AA38ZWZ1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Ubiquitin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_008800</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the 40S subunit of the ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Part of the 40S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the eukaryotic ribosomal protein eS31 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the ubiquitin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZWZ1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01803">
        <property type="entry name" value="Ubl_ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000008">
        <property type="entry name" value="Ubiquitin-40S ribosomal protein S27a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002906">
        <property type="entry name" value="Ribosomal_eS31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038582">
        <property type="entry name" value="Ribosomal_eS31_euk-type_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019954">
        <property type="entry name" value="Ubiquitin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019956">
        <property type="entry name" value="Ubiquitin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050158">
        <property type="entry name" value="Ubiquitin_ubiquitin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10666">
        <property type="entry name" value="UBIQUITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01599">
        <property type="entry name" value="Ribosomal_S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00348">
        <property type="entry name" value="UBIQUITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01402">
        <property type="entry name" value="Ribosomal_S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00299">
        <property type="entry name" value="UBIQUITIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="9" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="12">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="Small ribosomal subunit protein eS31" evidence="13">
        <location>
            <begin position="102"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00035123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9696766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="156" mass="17716" checksum="1E65C770FFD64DAD" modified="2024-01-24" version="1">MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGAKKRKKKTYTKPKKIKHKKKKVKLAVLQFYKVDDSGKVQRLRKECPNAECGAGTFMANHFDRHYCGKCGLTYVYQKAGGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZXU4</accession>
    <name>A0AA38ZXU4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Agglutinin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_009110</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aerolysin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZXU4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00257">
        <property type="entry name" value="beta-trefoil_FSCN-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20216">
        <property type="entry name" value="PFM_HFR-2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.15.10">
        <property type="entry name" value="Proaerolysin, chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055267">
        <property type="entry name" value="Aerolysin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008998">
        <property type="entry name" value="Agglutinin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036242">
        <property type="entry name" value="Agglutinin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053237">
        <property type="entry name" value="Natterin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39244:SF5">
        <property type="entry name" value="NATTERIN-3-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39244">
        <property type="entry name" value="NATTERIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01117">
        <property type="entry name" value="Aerolysin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07468">
        <property type="entry name" value="Agglutinin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00791">
        <property type="entry name" value="Agglutinin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56973">
        <property type="entry name" value="Aerolisin/ETX pore-forming domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50382">
        <property type="entry name" value="Agglutinin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Agglutinin" evidence="3">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="Agglutinin" evidence="3">
        <location>
            <begin position="129"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9697187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="437" mass="49741" checksum="E2B6ED10B94EECBA" modified="2024-01-24" version="1">MALPRYVVLKSKYNNKYLRYIHEDVQIHGFLQFSGEEVVTPYSKYQVEMAKNGKGLVHIRCCYNNKYWVRWSKNHWWIVAGADEPDEDQSSWSCTFLPPPYGSCLFAGSTSPDNDLRDVCTIIDWESLLLLPKHIAFKGDNGYYLNARTIEGHPYLEFASSDIGDPTVGNEVFTTHDGSVHIKSDYFGRFWRRNPNWIWADSDDSTTNNPDTLFWPIRVDKNVVALRNLGNNNFCKRLTTEGKISCLNAGVSTISREARLEVAELVLSRNIYNVNFRLMDARIYDQRVIVMTTGEAINMTQEPHTQQVKLSYTETKSRTWKGSVSLKLGVKITMESGVPFIADGKLEISSEFSSTYERGETESVTTAMETVYNVTVPTMTKVTVSMIATQGSCDVPFSYSQRDTLTDGKNVVYNMDDGVYVGVNCFNVKYHTKEEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZY70</accession>
    <name>A0AA38ZY70_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Methenyltetrahydrofolate cyclohydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_009296</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NADP(+) = (6R)-5,10-methenyltetrahydrofolate + NADPH</text>
            <dbReference type="Rhea" id="RHEA:22812"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.5.1.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZY70"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004477">
        <property type="term" value="F:methenyltetrahydrofolate cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004488">
        <property type="term" value="F:methylenetetrahydrofolate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009853">
        <property type="term" value="P:photorespiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01080">
        <property type="entry name" value="NAD_bind_m-THF_DH_Cyclohyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000006">
        <property type="entry name" value="Bifunctional protein FolD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10860:FF:000005">
        <property type="entry name" value="C-1-tetrahydrofolate synthase, cytoplasmic, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01576">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000672">
        <property type="entry name" value="THF_DH/CycHdrlase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020630">
        <property type="entry name" value="THF_DH/CycHdrlase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020867">
        <property type="entry name" value="THF_DH/CycHdrlase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020631">
        <property type="entry name" value="THF_DH/CycHdrlase_NAD-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48099">
        <property type="entry name" value="C-1-TETRAHYDROFOLATE SYNTHASE, CYTOPLASMIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48099:SF13">
        <property type="entry name" value="METHYLENETETRAHYDROFOLATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00763">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02882">
        <property type="entry name" value="THF_DHG_CYH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00085">
        <property type="entry name" value="THFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00766">
        <property type="entry name" value="THF_DHG_CYH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0601">Photorespiration</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tetrahydrofolate dehydrogenase/cyclohydrolase catalytic" evidence="12">
        <location>
            <begin position="74"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="Tetrahydrofolate dehydrogenase/cyclohydrolase NAD(P)-binding" evidence="13">
        <location>
            <begin position="210"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00052194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00058319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00061364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9697411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="362" mass="39035" checksum="DB8CA8B17E1FB95E" modified="2024-01-24" version="1">MGGGGGGAAWRTLGKKWLHSTRALTHTRIPPNSLLSLDLPDVWIPNTNYTSTANTNCRFIPSSRIPNEHMATVIDGKSIADDVKSGIAWEICRMKNAIGKLPGLAVLLVGKRRDSQTFIRIKIKACNEVGIASFIAELPEDCTEDEIINVISSYNENPSVHGIIVQLPLPQHLDQEKIVNAVSLEKDVDGFHPTNIGNLAMRGREPLFIPCAPMVCIELLLRSGVEIIGKKAAVIGRSQIAGLPTSLLLQRHNATVSTVHAFTKNPEQITSQADIVVTDVGVPNMVRGHWLKQGAVVVDMGTNLVKDPSCRQGYRVTGDVCFEEAIGVASAITPVPGGVGPVTISMLLSNTLDSAKRAYRFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZYD8</accession>
    <name>A0AA38ZYD8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="2">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_009429</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the peptidase C19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZYD8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02661">
        <property type="entry name" value="Peptidase_C19E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000078">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF663">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="4">
        <location>
            <begin position="107"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="48"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="504"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="672"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="765"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="794"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="863"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="949"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="17"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="80"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="765"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="798"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="826"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="882"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="976"/>
            <end position="985"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9697596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="997" mass="108990" checksum="2FC7C5B3B1B743C9" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZZT4</accession>
    <name>A0AA38ZZT4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CFA20 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_009204</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZZT4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040441">
        <property type="entry name" value="CFA20/CFAP20DC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007714">
        <property type="entry name" value="CFA20_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12458">
        <property type="entry name" value="ORF PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05018">
        <property type="entry name" value="CFA20_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CFA20" evidence="1">
        <location>
            <begin position="1"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9697304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="205" mass="23798" checksum="3025BABA894AD1E4" modified="2024-01-24" version="1">MFKNTLQSGILLTTKELWEERYVEASRVVNGQIKRTQDEDIHSNVLEIVGSNVQSTYITCPADPAATLGIKLLFLVMIVKILKKYFTFEILVLDYKNVRRRFRASNFQLKCCLCESVFFMLWAMTALTGVKPYICSLPLRMGERWNTIQSNLANFTRRAYGTDYVETLLVQVHANCQLRRIYFSDPLCSEELPPEFKLYLPMQKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A011</accession>
    <name>A0AA39A011_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_009324</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A011"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47938:SF23">
        <property type="entry name" value="PENTACOTRIPEPTIDE-REPEAT REGION OF PRORP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47938">
        <property type="entry name" value="RESPIRATORY COMPLEX I CHAPERONE (CIA84), PUTATIVE (AFU_ORTHOLOGUE AFUA_2G06020)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12854">
        <property type="entry name" value="PPR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13812">
        <property type="entry name" value="PPR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="12"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="247"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="282"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="317"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="352"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="387"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="442"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="477"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="512"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="547"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="582"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="617"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="723"/>
            <end position="757"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9697453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="867" mass="97197" checksum="F4FF698156DEFE27" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A3L2</accession>
    <name>A0AA39A3L2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_005606</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9699822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A3L2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000298">
        <property type="entry name" value="Calcium-binding protein CP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050145">
        <property type="entry name" value="Centrin_CML-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23050">
        <property type="entry name" value="CALCIUM BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01697">
        <property type="entry name" value="PARVALBUMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="13"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="51"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="92"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9699822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="163" mass="17670" checksum="6C50B6FF78F9492D" modified="2024-01-24" version="1">MCPTGSSLPQATAVKSEFRPAFDVLDADRDGKISREDLRSFYSGFPGGVTGDEEAIGSMMSVADSNKDGFVEYDEFERVLGCRRSPRNKGHGVAGVMEDVFKVMDRDGDGKVGLEDLKSYMNWAGFSATEEDIKAMIRLGGGDEDSGVSYDGLLKILAVDYVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A481</accession>
    <name>A0AA39A481_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase Ty1/copia-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_006133</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A481"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase Ty1/copia-type" evidence="2">
        <location>
            <begin position="261"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="104"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9700668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="522" mass="58675" checksum="60A90D330BF73C43" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A4G9</accession>
    <name>A0AA39A4G9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">dihydrolipoyl dehydrogenase</fullName>
            <ecNumber evidence="3">1.8.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">PVL29_005570</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="23">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Lipoamide dehydrogenase is a component of the plastidial pyruvate dehydrogenase complex (PDC).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>N(6)-[(R)-dihydrolipoyl]-L-lysyl-[protein] + NAD(+) = N(6)-[(R)-lipoyl]-L-lysyl-[protein] + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15045"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="EC" id="1.8.1.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="17">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Homodimer. Part of the plastidial pyruvate dehydrogenase complex (PDC) containing multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast stroma</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 19">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.4" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9699763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A4G9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009570">
        <property type="term" value="C:chloroplast stroma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045252">
        <property type="term" value="C:oxoglutarate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004148">
        <property type="term" value="F:dihydrolipoyl dehydrogenase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006103">
        <property type="term" value="P:2-oxoglutarate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046685">
        <property type="term" value="P:response to arsenic-containing substance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.30:FF:000001">
        <property type="entry name" value="Dihydrolipoyl dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000043">
        <property type="entry name" value="Putative dihydrolipoyl dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050151">
        <property type="entry name" value="Class-I_Pyr_Nuc-Dis_Oxidored"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001100">
        <property type="entry name" value="Pyr_nuc-diS_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012999">
        <property type="entry name" value="Pyr_OxRdtase_I_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22912:SF151">
        <property type="entry name" value="DIHYDROLIPOYL DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22912">
        <property type="entry name" value="DISULFIDE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000350">
        <property type="entry name" value="Mercury_reductase_MerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="FAD/NAD-linked reductases, dimerisation (C-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00076">
        <property type="entry name" value="PYRIDINE_REDOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 17 19" id="KW-0274">FAD</keyword>
    <keyword evidence="5 19" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10 17" id="KW-0520">NAD</keyword>
    <keyword evidence="17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 19" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0934">Plastid</keyword>
    <keyword evidence="12 19" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="22">
        <location>
            <begin position="85"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="domain" description="Pyridine nucleotide-disulphide oxidoreductase dimerisation" evidence="21">
        <location>
            <begin position="439"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="58"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="16">
        <location>
            <position position="541"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="226"/>
            <end position="228"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="263"/>
            <end position="270"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="359"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="405"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="411"/>
            <end position="414"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="18">
        <location>
            <begin position="122"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00059226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00065519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU003691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAJ9699763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="571" mass="60481" checksum="E7DBCF376942577A" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A5Q5</accession>
    <name>A0AA39A5Q5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Calcineurin B-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_006711</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Acts as a calcium sensor. CBL proteins interact with CIPK serine-threonine protein kinases. Binding of a CBL protein to the regulatory NAF domain of a CIPK protein lead to the activation of the kinase in a calcium-dependent manner.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer. Interacts with CIPK.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the calcineurin regulatory subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A5Q5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019900">
        <property type="term" value="F:kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000073">
        <property type="entry name" value="calcineurin B-like protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045198">
        <property type="entry name" value="CNBL1-10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23056">
        <property type="entry name" value="CALCINEURIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23056:SF44">
        <property type="entry name" value="CALCINEURIN B-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00450">
        <property type="entry name" value="RECOVERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="67"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="104"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="148"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU369080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9701475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="213" mass="24473" checksum="87C44A3213EA81B0" modified="2024-01-24" version="1">MGCFQSTARKQFPGHEDPVILASQTAFSVSEVEALFELFKSISSSVIDDGLINKEEFQLALFKNRKKENLFANRIFDLFDVKRKGVIDFGDFVRSLNVFHPNAPQEDKIDFSFKLYDLDSTGFIERQEVKQMLIALLCESEMKLADETIEIILDKTFLEADVNQDGRIDKSEWQNFVSRNPSLLKIMTLPYLRDITTTFPSFVFNSEVDEIAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A6P8</accession>
    <name>A0AA39A6P8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_003966</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9701988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A6P8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000554">
        <property type="entry name" value="protein indeterminate-domain 12-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000131">
        <property type="entry name" value="protein indeterminate-domain 5, chloroplastic-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055187">
        <property type="entry name" value="C2CH-3rd_BIRD-IDD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055185">
        <property type="entry name" value="C2CH-4th_BIRD-IDD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055186">
        <property type="entry name" value="C2H2-2nd_BIRD-IDD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031140">
        <property type="entry name" value="IDD1-16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10593:SF218">
        <property type="entry name" value="C2H2-TYPE ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10593">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE RIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22995">
        <property type="entry name" value="C2CH-3rd_BIRD-IDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22992">
        <property type="entry name" value="C2CH-4th_BIRD-IDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22996">
        <property type="entry name" value="C2H2-2nd_BIRD-IDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="80"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="9"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="16"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="27"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9701988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="528" mass="57100" checksum="41F81B4C9B37021B" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A8Q6</accession>
    <name>A0AA39A8Q6_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_004291</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A8Q6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053350">
        <property type="entry name" value="CV_Inducer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37210">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37210:SF2">
        <property type="entry name" value="PROTEIN CHLOROPLAST VESICULATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="9"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9702477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="143" mass="15674" checksum="9B26A8F194169E9C" modified="2024-01-24" version="1">MAFSAGCCLNLSPPPSGPSPSSSRSSTKTDQVSWPRKENSLKSKCLVGLTCMIISLEMSNLMSGEGLAIAQDLQLTGERKEATRWSDKRMCPPWQLNSLETIVPENLPRPSARRRWEAVGHSTTAPAVKILARIHTKSDCFSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AA67</accession>
    <name>A0AA39AA67_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Aconitate hydratase</fullName>
            <shortName evidence="10">Aconitase</shortName>
            <ecNumber evidence="3 10">4.2.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_005196</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>citrate = D-threo-isocitrate</text>
            <dbReference type="Rhea" id="RHEA:10336"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:16947"/>
            <dbReference type="EC" id="4.2.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the aconitase/IPM isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.3" evidence="3 10"/>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AA67"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003994">
        <property type="term" value="F:aconitate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006101">
        <property type="term" value="P:citrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006102">
        <property type="term" value="P:isocitrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01586">
        <property type="entry name" value="AcnA_IRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01580">
        <property type="entry name" value="AcnA_IRP_Swivel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.19.10:FF:000001">
        <property type="entry name" value="Aconitate hydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.499.10:FF:000002">
        <property type="entry name" value="Aconitate hydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.499.10:FF:000005">
        <property type="entry name" value="cytoplasmic aconitate hydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="entry name" value="Aconitase, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="entry name" value="Aconitase, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044137">
        <property type="entry name" value="AcnA_IRP_Swivel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006249">
        <property type="entry name" value="Aconitase/IRP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018136">
        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01341">
        <property type="entry name" value="aconitase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006757">
        <property type="entry name" value="PRK09277.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009520">
        <property type="entry name" value="PRK12881.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11670">
        <property type="entry name" value="ACONITASE/IRON-RESPONSIVE ELEMENT FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="Aconitase iron-sulfur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52016">
        <property type="entry name" value="LeuD/IlvD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00450">
        <property type="entry name" value="ACONITASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01244">
        <property type="entry name" value="ACONITASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6 10" id="KW-0408">Iron</keyword>
    <keyword evidence="7 10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8 10" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aconitase/3-isopropylmalate dehydratase large subunit alpha/beta/alpha" evidence="11">
        <location>
            <begin position="65"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="domain" description="Aconitase A/isopropylmalate dehydratase small subunit swivel" evidence="12">
        <location>
            <begin position="697"/>
            <end position="824"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9703821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="900" mass="97934" checksum="1F35500B99AE5F69" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ACE3</accession>
    <name>A0AA39ACE3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="1">2.3.2.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_002842</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="1"/>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ACE3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd23837">
        <property type="entry name" value="UBCc_UBE2O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000028">
        <property type="entry name" value="Probable ubiquitin-conjugating enzyme E2 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057732">
        <property type="entry name" value="SH3-A_UBE2O"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057733">
        <property type="entry name" value="UBE2O-like_SH3-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057734">
        <property type="entry name" value="UBE2O-like_SH3-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057735">
        <property type="entry name" value="UBE2O-like_tSH3-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46116">
        <property type="entry name" value="(E3-INDEPENDENT) E2 UBIQUITIN-CONJUGATING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46116:SF21">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2 23-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23048">
        <property type="entry name" value="SH3-A_UBE2O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23043">
        <property type="entry name" value="SH3-B_UBE2O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23044">
        <property type="entry name" value="SH3-C_UBE2O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23046">
        <property type="entry name" value="tSH3-B_UBE2O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="7">
        <location>
            <begin position="903"/>
            <end position="1063"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="82"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="617"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="731"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="783"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="86"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="124"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="625"/>
            <end position="640"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9704465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1154" mass="127120" checksum="5E2CA41CB5F15480" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AD66</accession>
    <name>A0AA39AD66_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_003098</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AD66"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000775">
        <property type="entry name" value="BnaA09g28200D protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001048">
        <property type="entry name" value="BnaA09g40460D protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045182">
        <property type="entry name" value="JINGUBANG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22844">
        <property type="entry name" value="F-BOX AND WD40 DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22844:SF370">
        <property type="entry name" value="OS12G0594000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="168"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="210"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="257"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="300"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9704885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="410" mass="45302" checksum="BDB9949732135787" modified="2024-01-24" version="1">MNRRSNVSMDSSVHPSSGENSPLIMSPWNHPSSPIAKSPWTRSSSPMGLRQNGLIGSMVREEGHVYSLAATGNLLYTGSDSKNIRVWKNLKAYSGFKSNSGLVKAIVISGEKIFTGHQDGKIRVWRVSPKNPKLHKRIGSLPTFKDYLKSSINPKNYVKVRRKRSVLRIKHFDAVSCLNLDQDQGLLYSGSWDKTLKVWRISDSKCLESIRAHDDAINAVVAGFAGLVFTGSADGTVKVWHRQLHGKGTKHFFLQTLLNQETAVTSLAVNTEGAIIYCGSSDGLVNFWQREKHLNHGGVLRGHKHAVLCLATAGNLVFSGSADKSICVWRREGGVHTCLSVLTGHTGPVKCLAVEEDQESTKGDQRWIVYSGSLDKSVKIWRVWEQAPDMQQMKLMAQNYVPSPLSSPAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AEY3</accession>
    <name>A0AA39AEY3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Short-chain dehydrogenase TIC 32, chloroplastic-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_001694</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AEY3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05327">
        <property type="entry name" value="retinol-DH_like_SDR_c_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320:SF114">
        <property type="entry name" value="OS03G0115700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320">
        <property type="entry name" value="RETINOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9706292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="336" mass="36540" checksum="18A0D4679295C6BA" modified="2024-01-24" version="1">MLDALKYLLGSFGASGYGSKSTAEEVTENCPDLGSITAVITGATSGIGAETARVLAKRGARLVLPARSLKAAEEAKARIVAELPNSEIVVMALDLSSLDSVRCFVSEFESLDLPLNLLINNAGKFTHEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIETAKKTGVQGRIVNVSSTIHSWFSGDVIRYLGLITRNKSQYDATRAYAVSKLANVLHTKELAQRLKQMDANVTVNCVHPGIVRTRLTRETEGIVTDLIFFLTSKFLKTIPQAAATTCYVATHPRLVNVSGKYFADCNEAWTSKLGSNSQEASRLWSASEIMTARDSRAIFDPLDASD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AFP2</accession>
    <name>A0AA39AFP2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PVL29_003540</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the leguminous lectin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AFP2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002229">
        <property type="term" value="P:defense response to oomycetes"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06899">
        <property type="entry name" value="lectin_legume_LecRK_Arcelin_ConA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000240">
        <property type="entry name" value="Lectin-domain containing receptor kinase A4.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050528">
        <property type="entry name" value="L-type_Lectin-RKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019825">
        <property type="entry name" value="Lectin_legB_Mn/Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001220">
        <property type="entry name" value="Legume_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27007">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00139">
        <property type="entry name" value="Lectin_legB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00307">
        <property type="entry name" value="LECTIN_LEGUME_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0430">Lectin</keyword>
    <keyword evidence="15 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="233"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
            <begin position="284"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="313"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAJ9705538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="611" mass="67980" checksum="74F2B13ED7DC6FFC" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AHK5</accession>
    <name>A0AA39AHK5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_000042</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9707781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AHK5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43689:SF8">
        <property type="entry name" value="ALPHA_BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43689">
        <property type="entry name" value="HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="85"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9707781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="340" mass="38121" checksum="A7F05B9657022A91" modified="2024-01-24" version="1">MFTLVAGSVPAGLGATAKRGSLWRFRATADVFPSFIPKEVERIKDPFARKLATRIERLPVQLSSSNSCIMSSCVKPLSPSKTSPVVLLHGFDSSCLEWRYTYPLLEEAGLETWAVDILGWGFSDLERLPSCDVATKRDHFYQLWKSHIKRPMILVGPSLGAAVAIDFTANHPEAVDKLVLIDASVYTEGTGNLMKLPRAVAYAGVYILKSIPLRFYANVLAFKSISFTRSSDWTKVGRLHCLYPWWEDATVNFMISGGYNVSSQIQQVKKKTLIIWGEDDQIISNKLAVRLHCELPNAIIRQIPDCGHLPHVEKPHSVSKLIMEFVQEDSYKEAQCISQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AHR7</accession>
    <name>A0AA39AHR7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_002466</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AHR7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9707449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="269" mass="31116" checksum="F325E014D3B36E98" modified="2024-01-24" version="1">MAKSKKSSKQYSLQKCVGPKEKYTALKGLFLGSREKPLRELEEGTKSDSLLCERSLEEKDNKKENEHYVDTLLVDKLCTKDMNGKNEDACATSIEMHIIPNENADILPLLIKRIRKDYGKEPLQALCGIVEHIMRTHTSRERKPSKFKVSLFVHNLRKMGKRKVSEIEFCQTFFKLYNIRKNVFQFSIDWAPPILTQDNGWDCGVHVIKHMQTFQNGDCIKASDLRNSVKIHQEIASDLVLHKRNREKQTILAIVCTNISTRAMRRLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AID4</accession>
    <name>A0AA39AID4_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_000315</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AID4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9708195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="258" mass="28739" checksum="A130CF1D3F22AA28" modified="2024-01-24" version="1">MGSSPRLQSLSLSGVGGLSLCRLQFFRTLPFVRLQMNILLLVKVRAQEGRSSMAENLALYRNFDKSAENVGQFQGRLGALFKTISVVNFFLEIVLSLVHLDLVGGDVKATVDLYFVRVDDLGGEACAEVDGEFGFSGAGGSHDNDDLVLGAITADRGIHASPPGSSEGGLYRRYEWERLNFAAIWMITGGDGEFHRNRNDGRFDGVVCAVLHRYITLRQRWHIEILAEMEWDENPVQLSLDRKIVVVEQRGMTFPSER</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AJX8</accession>
    <name>A0AA39AJX8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_000823</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AJX8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044660">
        <property type="entry name" value="IBH1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33124:SF9">
        <property type="entry name" value="TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33124">
        <property type="entry name" value="TRANSCRIPTION FACTOR IBH1-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="24"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="BHLH" evidence="7">
        <location>
            <begin position="66"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="9"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9709020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="131" mass="15023" checksum="E760AA815B7D1869" modified="2024-01-24" version="1">MARPCTTSSPPPPPPQPSPSLNNIYPSHLHLSSLISFLLFFFVGYQFGIPTVRARVVQVAMEGSKCKEMSGEKLRRRRRRSVRRKVKKLQRLIPGGRGLQPDRLFMRTADYILHLRLQVNVLQALSKIYKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AKL2</accession>
    <name>A0AA39AKL2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="1">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_000148</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5 1">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="1"/>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9707939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AKL2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044197">
        <property type="entry name" value="FKBP17-1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47860">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FKBP17-1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47860:SF1">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FKBP17-1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase FKBP-type" evidence="2">
        <location>
            <begin position="101"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9707939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="250" mass="27198" checksum="B81DEC9A9CBB95C0" modified="2024-01-24" version="1">MMKMVIIRWCAPPPALLLPHHPTARFSSPKPPSVVSAKSSSPASSSTTRRALSLSLIFSSFTCSYSSSSEAAASEFFELQGSGGVKALDLRTGSGEVPVDGDQVAVHYYGRLAAKQGWRFDSTYDHKDETGEPIPFVFTLGSGKLSSFPFPSPVYNVPSEESEPRRLVMQTVISGIEAAVRSMKVGSIRRVIVPPSQGYQNTSQEPIPPNFFDRQRLFTTIFNPTRLANGEGSTLGTLIFDIELVSLKHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AKS5</accession>
    <name>A0AA39AKS5_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_001022</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AKS5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901957">
        <property type="term" value="P:regulation of cutin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000099">
        <property type="entry name" value="MYB transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000001">
        <property type="entry name" value="MYB-related transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015495">
        <property type="entry name" value="Myb_TF_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10641">
        <property type="entry name" value="MYB FAMILY TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10641:SF1347">
        <property type="entry name" value="MYB TRANSCRIPTION FACTOR MIXTA-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="9"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="9"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="62"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="62"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9709351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="371" mass="41240" checksum="C2A5DBC874998A7A" modified="2024-01-24" version="1">MGRSPCCEKVGLKKGSWTPEEDQKLMSYIEEHGHGSWRALPAKAGLQRCGKSCRLRWTNYLRPDIKRGKFSLQEEQTIIQLHALLGNRWSAIATHLPKRTDNEIKNYWNTHLKKRLTKMGIDPVTHKPKSYALGSATGQSKDAANLSHMAQWESARLEAEARFVREYKLSPHPFQHQYKQGLSATALSITHSLAPPKTLDVVNAWQGLWSSGSTNDCAVFGDTLFNSSENALPAALPSSTLGLGVNENSMEKGENDWKHCFERQNKVPEFIKDNATPFPDMQYSTDESTWIADSVGNNNIVISCATFNVDGFSHHLLPENSSLSFGEDSDNANANCTAGSAGGGFEENNHYWNNIISLLDSSSAYYSLENV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AL77</accession>
    <name>A0AA39AL77_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_001273</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AL77"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24126:SF40">
        <property type="entry name" value="ANKYRIN REPEAT FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24126">
        <property type="entry name" value="ANKYRIN REPEAT, PH AND SEC7 DOMAIN CONTAINING PROTEIN SECG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="221"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="447"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="582"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="582"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="613"/>
            <end position="624"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9709713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="671" mass="74320" checksum="676F2ADA9C21338A" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ALZ7</accession>
    <name>A0AA39ALZ7_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_001113</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ALZ7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9709475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="127" mass="13961" checksum="C39AF77454875843" modified="2024-01-24" version="1">MEKKPKILLVESLEIISIPSVETIILKSVLFISFTVAGFLMVLPSEQIDGNPVPTIIFKGHPSLFHAFLISVSFAFNGAFTALMMENQSKFARFCGYYSIASMASAISVLMFAIFRQVGQQLQTSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AP37</accession>
    <name>A0AA39AP37_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_001033</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">May transport inorganic phosphate (Pi).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SYG1 (TC 2.A.94) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AP37"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000822">
        <property type="term" value="F:inositol hexakisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016036">
        <property type="term" value="P:cellular response to phosphate starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14476">
        <property type="entry name" value="SPX_PHO1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004342">
        <property type="entry name" value="EXS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034092">
        <property type="entry name" value="PHO1_SPX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10783:SF4">
        <property type="entry name" value="PHOSPHATE TRANSPORTER PHO1 HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10783">
        <property type="entry name" value="XENOTROPIC AND POLYTROPIC RETROVIRUS RECEPTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03124">
        <property type="entry name" value="EXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03105">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51380">
        <property type="entry name" value="EXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="383"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="424"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="466"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="507"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="533"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="595"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="653"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="697"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="domain" description="SPX" evidence="13">
        <location>
            <begin position="1"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="EXS" evidence="12">
        <location>
            <begin position="591"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="155"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="155"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="168"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00043939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9709364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="786" mass="91387" checksum="563C14C810C446E2" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AQD7</accession>
    <name>A0AA39AQD7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_001309</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AQD7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005162">
        <property type="entry name" value="Retrotrans_gag_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34482">
        <property type="entry name" value="DNA DAMAGE-INDUCIBLE PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34482:SF36">
        <property type="entry name" value="RETROTRANSPOSON GAG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03732">
        <property type="entry name" value="Retrotrans_gag"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Retrotransposon gag" evidence="2">
        <location>
            <begin position="118"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="Retrotransposon gag" evidence="2">
        <location>
            <begin position="333"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="252"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="252"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="266"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9709783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="429" mass="50173" checksum="A5FF55E36526FB3F" modified="2024-01-24" version="1">MVERTRRGRSERDEEFESVREELREVRRELREAAQLMRGQGSRRLGGTQGQEDSDHSHKRSRSERPVMSQMEAMKRFMVMQPPSFNGEPNAEVAEHWLRRMKRILVGLDIPEERRVSLAAYMLVDKADFWWESMKRVYDTEVMTWEKFEKIFLSNFFGKVAKHAKRMEFEHLIQGTMSVLEYESCFSELSRFALGMISEEGEKARRFQQGLRPAIRNRLVPLAIRDYSELVKRALLVEQDIEETNQIWVQMGDRKGKQRMKESSQRRSQGPQQRQRGQQFEGHSSFYAGGEQSAQRAATARVCYGCGAGDHLWRACPLWGAQQARPQPQGGSQHAKRMEFEHLIQGTMSVLEYESRFSELSRFALGMISEEGEKARRFQQGLRFAIRNRLVPLAIRDYSELVKRALLVEQDIEETNQIREQRGKPTKLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AR67</accession>
    <name>A0AA39AR67_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_001296</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AR67"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025753">
        <property type="entry name" value="AAA_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058017">
        <property type="entry name" value="At3g28540-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050747">
        <property type="entry name" value="Mitochondrial_chaperone_BCS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23070">
        <property type="entry name" value="BCS1 AAA-TYPE ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14363">
        <property type="entry name" value="AAA_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25568">
        <property type="entry name" value="AAA_lid_At3g28540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA-type ATPase N-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase At3g28540-like C-terminal" evidence="3">
        <location>
            <begin position="224"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9709754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="301" mass="34214" checksum="2165641BE47C70B6" modified="2024-01-24" version="1">MYKACEIFLHTKIPASVQKLKVFQAPEGKNLSIAIGEGEKAIDIFEGIQVKWEMVYTKKQSNEAIDYESRSMELSFPKKNMKKILSSYLPYVVDRSEAFIEENKVLKLYSYCGSWESTNLNHPSTFETLAMDSKLKQDLDQFVKRKKHYKRSSPVSVATQEFRRLLVSIRNQSILVIEDIDCSSELQGQQAEGHNLNDSQLMLYELLNSIDGLWSSCGDKQIITLASNYLGIKDHKFPEIEKLIVEVEVTPAAIAEELMKSEEAGIALRRLVVFLKRVMTVQNEATDGEDTNEKGNESPSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D338</accession>
    <name>A0AA39D338_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PTC1-like winged helix-turn-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_026636</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D338"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051177">
        <property type="term" value="P:meiotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044221">
        <property type="entry name" value="DYAD/AMEIOTIC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059080">
        <property type="entry name" value="WHD_PTC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46740">
        <property type="entry name" value="PROTEIN DYAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46740:SF2">
        <property type="entry name" value="PROTEIN DYAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25874">
        <property type="entry name" value="WHD_plant_repro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PTC1-like winged helix-turn-helix" evidence="2">
        <location>
            <begin position="229"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="164"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="173"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="192"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9670208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="362" mass="41866" checksum="88C8C978C07F94E7" modified="2024-01-24" version="1">MEEVSSFYEIDHSRLPPDSPSQLHPCRVVMVSEKTEHNVSVRFPSRLSIREHFRDKASGEVNGKMYPALDEKWEMGLNIAGQVLFRQIPTEEFSEQRNLSSFWSVQQLETEKISIPSSDGGLGDLTSKKGKCWSELKCEGLVKWGMRRKVRFISRYFEDNSEHLTTEVKEEEKGDEEETEEAANFDMPETRRSRKRKRHTFNRTQKAKREKQQQSSGYTGRILKNSKDRWSAERYKIAEENMLKILKAKGAVFGNPILRPELRQEARKKIGDTGLLDHLLKHMAGKVAPGGAERFRRRHNADGAMEYWLESADLVSIRREAGVQDPYWTPPLGWKPGDSPTQDPVCANELKRLKEEMAQMKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D6T3</accession>
    <name>A0AA39D6T3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Geranylgeranyl transferase type-2 subunit alpha</fullName>
            <ecNumber evidence="6">2.5.1.60</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Geranylgeranyl transferase type II subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_023530</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the transfer of a geranyl-geranyl moiety from geranyl-geranyl pyrophosphate to cysteines occuring in specific C-terminal amino acid sequences.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>geranylgeranyl diphosphate + L-cysteinyl-[protein] = S-geranylgeranyl-L-cysteinyl-[protein] + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21240"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11537"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57533"/>
            <dbReference type="ChEBI" id="CHEBI:86021"/>
            <dbReference type="EC" id="2.5.1.60"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the protein prenyltransferase subunit alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.60" evidence="6"/>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D6T3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005968">
        <property type="term" value="C:Rab-protein geranylgeranyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004663">
        <property type="term" value="F:Rab geranylgeranyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097354">
        <property type="term" value="P:prenylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.120:FF:000013">
        <property type="entry name" value="Geranylgeranyl transferase type-2 subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000756">
        <property type="entry name" value="Rab geranylgeranyl transferase like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.120">
        <property type="entry name" value="Protein prenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002088">
        <property type="entry name" value="Prenyl_trans_a"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11129:SF2">
        <property type="entry name" value="GERANYLGERANYL TRANSFERASE TYPE-2 SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11129">
        <property type="entry name" value="PROTEIN FARNESYLTRANSFERASE ALPHA SUBUNIT/RAB GERANYLGERANYL TRANSFERASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01239">
        <property type="entry name" value="PPTA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48439">
        <property type="entry name" value="Protein prenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51147">
        <property type="entry name" value="PFTA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0637">Prenyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9674033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="704" mass="80308" checksum="09F02707D48DDD2F" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D753</accession>
    <name>A0AA39D753_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_023026</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0496 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D753"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007749">
        <property type="entry name" value="DUF677"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31113:SF5">
        <property type="entry name" value="OS04G0405700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31113">
        <property type="entry name" value="UPF0496 PROTEIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="9"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9673164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="349" mass="39742" checksum="624867020CB856D9" modified="2024-01-24" version="1">MLQCLSLKSSASTSTTTAPAVENSREDTPRSISQTSPTVNLTREYTLAVQTNSYNEIWSKIHVRHQDDYTNTDPDQDTDGEREEDVHQLRLAHVLQPDRECVQDALRHARSNTLTRLVSDYFDHSENTSQLCLLLHRSVYHAHSLYAPLHDLLDILPLDSDSLTQSQCNQAFDVFLQFDSLDNPFPCPDSHNFHDMRRCFSQLKEQLHGHIRKSRSKIATHTLVALISPLFTAFLPPKLSKKELAHGAQLDAAARGTYVLCHDLDTIDSLVAWLHTAVDGDKRLIRLGLGGGRDKHTIQEVAKHLRKNHRNFLHQLKDLEEHICLCFTTVNRARSLLLREIDLRHSSNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D7P8</accession>
    <name>A0AA39D7P8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein DETOXIFICATION</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Multidrug and toxic compound extrusion protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_023579</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D7P8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13132">
        <property type="entry name" value="MATE_eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045069">
        <property type="entry name" value="MATE_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="95"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="128"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="171"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="203"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="230"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="275"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="314"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="356"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="396"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="432"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9674111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="529" mass="57835" checksum="09A950B8092FC828" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D8L4</accession>
    <name>A0AA39D8L4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 13">Squalene monooxygenase</fullName>
            <ecNumber evidence="5 13">1.14.14.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_023606</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the stereospecific oxidation of squalene to (S)-2,3-epoxysqualene, and is considered to be a rate-limiting enzyme in steroid biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>squalene + reduced [NADPH--hemoprotein reductase] + O2 = (S)-2,3-epoxysqualene + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25282"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15440"/>
            <dbReference type="ChEBI" id="CHEBI:15441"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="1.14.14.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Terpene metabolism; lanosterol biosynthesis; lanosterol from farnesyl diphosphate: step 2/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Membrane</location>
            <topology evidence="2 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the squalene monooxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.17" evidence="5 13"/>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D8L4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004506">
        <property type="term" value="F:squalene monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009725">
        <property type="term" value="P:response to hormone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016126">
        <property type="term" value="P:sterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000074">
        <property type="entry name" value="Squalene monooxygenase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013698">
        <property type="entry name" value="Squalene_epoxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040125">
        <property type="entry name" value="Squalene_monox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10835">
        <property type="entry name" value="SQUALENE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10835:SF31">
        <property type="entry name" value="SQUALENE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08491">
        <property type="entry name" value="SE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13" id="KW-0274">FAD</keyword>
    <keyword evidence="6 13" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="domain" description="Squalene epoxidase" evidence="14">
        <location>
            <begin position="210"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU367121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9674155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="523" mass="56919" checksum="C9D4A20AD9AB363C" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D940</accession>
    <name>A0AA39D940_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BPS1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_023346</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D940"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31509">
        <property type="entry name" value="BPS1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="173"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9673732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="322" mass="36431" checksum="6E656821D545B5F3" modified="2024-01-24" version="1">MMDPSPTNSVNGFYSFLTRGIDDLERVFLSTNFMSIQFLQRVLSLLRSFHSQLTLLVQKLHLPVGEKWLDEYMDESSKLWEACHVLKSGISGMENYYSAGLNIAASLDSHRHLSPQLSRQVIRAISGCHIEAVGLEEGNRAMMETRIQPLSLRLDEKVSIESKLNGFNGFRGVLYAMRNVSSLLLMILLCGLVYCWPESNFLRGGYEGCLLFGSAFMISTARLQQRVAAEINQINGRPGILLYEFRRSKVAMDELRGELERRCSQGVVDWESEVGIGERVENLRGCFGVLRSGAENIIGQLDDFFDEIVEGREKLLNFCSHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DA38</accession>
    <name>A0AA39DA38_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Response regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_022451</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DA38"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009736">
        <property type="term" value="P:cytokinin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045279">
        <property type="entry name" value="ARR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43874">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43874:SF1">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR-LIKE APRR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="26"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9677468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="153" mass="17351" checksum="25C17842E00F4D05" modified="2024-01-24" version="1">MARNEIGDGGSNSKSSDSFIDRSNVRILLCDNDTKSSDEIFTLLCGCSYQVTAVRSARQVIDALNAEGHDIDIILAEVDLPMTKGMKMLKYIMRDKELRRIPVIMMSAQDEVSVVVKCLRLGAADYLVKPLRTNELLNLWTHMWRRRRMVCIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DBR5</accession>
    <name>A0AA39DBR5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Centromere protein X</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_022746</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CENP-X/MHF2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DBR5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071821">
        <property type="term" value="C:FANCM-MHF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051382">
        <property type="term" value="P:kinetochore assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031297">
        <property type="term" value="P:replication fork processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22921">
        <property type="entry name" value="HFD_CENP-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.130.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018552">
        <property type="entry name" value="CENP-X"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28680">
        <property type="entry name" value="CENTROMERE PROTEIN X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28680:SF1">
        <property type="entry name" value="CENTROMERE PROTEIN X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09415">
        <property type="entry name" value="CENP-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9677949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="105" mass="11492" checksum="2C0918890C4A3CCC" modified="2024-01-24" version="1">MDSANTGFDPDLIHAIFKLVWSRTALEREKNEGADALECEVGAATSKKNRPTSANANALKLSCELLRVFVIEAVERAATIAEAEGVNKIEATHLERILPQLLLDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DEV8</accession>
    <name>A0AA39DEV8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">chitinase</fullName>
            <ecNumber evidence="2">3.2.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_021551</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1-&gt;4)-beta-linkages in chitin and chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.14"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the glycosyl hydrolase 18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.14" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DEV8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008843">
        <property type="term" value="F:endochitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02877">
        <property type="entry name" value="GH18_hevamine_XipI_class_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000015">
        <property type="entry name" value="Acidic endochitinase SE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045321">
        <property type="entry name" value="Cts1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001579">
        <property type="entry name" value="Glyco_hydro_18_chit_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050542">
        <property type="entry name" value="Glycosyl_Hydrlase18_Chitinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45708:SF21">
        <property type="entry name" value="ACIDIC ENDOCHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45708">
        <property type="entry name" value="ENDOCHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01095">
        <property type="entry name" value="GH18_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0146">Chitin degradation</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041217008" description="chitinase" evidence="11">
        <location>
            <begin position="26"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="GH18" evidence="12">
        <location>
            <begin position="26"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9679657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="296" mass="31917" checksum="E8AF6B0CB3912C72" modified="2024-01-24" version="1">MARTPQSRALLISLSVLAFLQTSYAGGIAIYWGQNGIEGTLTETCNTGKYSYVNIAFLNTFGNGRTPEINLGGHCDPASNGCTSLSTDIRNCQNRGIKVMLSIGGGDGSYSLSSSKDAQKVANYLWNNFLGGKSSSRPLGDAVLDGIDFNIELGSTKYWDNLARALSGCGKRRRKVYLTAAPQCAFPDRFLGAALKTGLFDHVWVQFYNNAPCQYTTGNTNKLLNSWNRWTSSINSRIFLGLPASSAAAGGGFIPADVLTSQILPVIKRSPKYGGVMLWSYYDDQSGYASTIKSSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DGV4</accession>
    <name>A0AA39DGV4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PVL29_019252</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DGV4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000309">
        <property type="entry name" value="Leucine-rich repeat receptor protein kinase MSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000439">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase FLS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000445">
        <property type="entry name" value="MDIS1-interacting receptor like kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000400">
        <property type="entry name" value="Nuclear pore complex protein NUP107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051420">
        <property type="entry name" value="Ser_Thr_Kinases_DiverseReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48005">
        <property type="entry name" value="LEUCINE RICH REPEAT KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48005:SF16">
        <property type="entry name" value="MDIS1-INTERACTING RECEPTOR LIKE KINASE 2-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8 19" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041325986" description="non-specific serine/threonine protein kinase" evidence="19">
        <location>
            <begin position="29"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
            <begin position="370"/>
            <end position="638"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAJ9683606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="650" mass="72413" checksum="8368858CE93C5AE0" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DI61</accession>
    <name>A0AA39DI61_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_017720</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DI61"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019762">
        <property type="term" value="P:glucosinolate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009651">
        <property type="term" value="P:response to salt stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000020">
        <property type="entry name" value="Beta-glucosidase 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033132">
        <property type="entry name" value="GH_1_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001360">
        <property type="entry name" value="Glyco_hydro_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353:SF318">
        <property type="entry name" value="BETA-GLUCOSIDASE 31-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353">
        <property type="entry name" value="GLYCOSYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00232">
        <property type="entry name" value="Glyco_hydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00653">
        <property type="entry name" value="GLYCOSYL_HYDROL_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041306738" evidence="5">
        <location>
            <begin position="22"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9685778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="386" mass="43420" checksum="20E0DF2FBDFD6106" modified="2024-01-24" version="1">MAIQCSLVLGLLILASSLAWTEPVVASSFNRSSFPAGFIFGTASASYQYEGAAKEGGRGPSIWDTFSHKYPDRIIDGSNGDVAENFYHRYKEDVHTMKELGMDAFRFSISWSRVLPCGKLSGGVNKKGINFYNNLINELLSKGLQPYVTLFHWDLPQALEDEYGGFLSPHIINDFRDFAELCFKEFGDRVKYWITLNEPWSFSYGGYNEGILAPGRCSKWVNGACRAGNSATEPYKIGHHLLLSHSAAVKVYKKKYQASQKGKIGITLVSHWMVPYSNQKVDKKAARRALDFMLGWFMNPLTYGDYPHSMRKLVGRRLPKFTPKQSMLIKGSFDFLGLNYYTANYAAHVPVANTVNVSYSTDSLVKLTSKFHIDRNGIPIGPTRWC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DLM5</accession>
    <name>A0AA39DLM5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DDE Tnp4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_014029</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HARBI1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DLM5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045249">
        <property type="entry name" value="HARBI1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930:SF244">
        <property type="entry name" value="OS05G0593000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE Tnp4" evidence="9">
        <location>
            <begin position="298"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="21"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9688075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="509" mass="57200" checksum="8A1433670E382E50" modified="2024-01-24" version="1">MESTFLLDDFSHFYSFFQDSGNSIPDDSAGVGGSGGGGRKRRRGSAGGSGDESGALKEILASLLLLDEEEKLDQDKWVLDCQNERALFEENHRQRAQAMNEYRADMVERFGEMEESESSRVKRARRSASAVAAGVAAAASSSDNVGSESSSQPVGHHRRLWVKDRSKAWWEWCSHPDFPEEEFRRAFRMSRATFDMICDELDSAVTKKDTMLRLAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCAAIKTVLMPKFLQWPDDEKLKMVKDEFESISGIPNVGGSMYTTHIPIIAPKVNVAAYFNKRHTERNQKTSYSVTVQGVVDPKGVFTDVSIGWPGSMSDEKVLEQSSLYQRANSGFLRDVWVVGGSGYPLLDWVMVPYTHQNLTWTQHAFNEKTGDIQKVAKEAFARLKRRWSCLQKRTEVKLLDLPVVLGACCVLHNICQMRNEEMDPELQFELFDDEMIPENGLRSASSAQARDRIAHDLLHHGLAGNAFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DM19</accession>
    <name>A0AA39DM19_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">G-patch domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_014490</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DM19"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000467">
        <property type="entry name" value="G_patch_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47423">
        <property type="entry name" value="G-PATCH DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47423:SF2">
        <property type="entry name" value="PROTEIN SQS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01585">
        <property type="entry name" value="G-patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00443">
        <property type="entry name" value="G_patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50174">
        <property type="entry name" value="G_PATCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G-patch" evidence="2">
        <location>
            <begin position="636"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="223"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="285"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="437"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="528"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="18"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="56"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="288"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="439"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="533"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="569"/>
            <end position="584"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9688869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="682" mass="73180" checksum="773C2E5A1A3FAA38" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DM56</accession>
    <name>A0AA39DM56_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_014314</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DM56"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000021">
        <property type="entry name" value="Zinc finger CCCH domain-containing protein 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041686">
        <property type="entry name" value="Znf-CCCH_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15725:SF0">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 32-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15725">
        <property type="entry name" value="ZN-FINGER, C-X8-C-X5-C-X3-H TYPE-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14608">
        <property type="entry name" value="zf-CCCH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15663">
        <property type="entry name" value="zf-CCCH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="8">
        <location>
            <begin position="6"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="8">
        <location>
            <begin position="37"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="8">
        <location>
            <begin position="87"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="6">
        <location>
            <begin position="6"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="6">
        <location>
            <begin position="37"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="6">
        <location>
            <begin position="87"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="132"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="390"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="428"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="132"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="435"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="447"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="490"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9688600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="545" mass="61795" checksum="1477E1F1578A7F78" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DMW9</accession>
    <name>A0AA39DMW9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_012456</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9689784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DMW9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001070">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At1g11630, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:002935">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At1g61870, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050667">
        <property type="entry name" value="PPR-containing_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47939:SF9">
        <property type="entry name" value="(WILD MALAYSIAN BANANA) HYPOTHETICAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47939">
        <property type="entry name" value="MEMBRANE-ASSOCIATED SALT-INDUCIBLE PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="200"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="270"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="305"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="340"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9689784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="422" mass="47741" checksum="7A2F1770877BF570" modified="2024-01-24" version="1">MESIVSLSKAKALIFQYHFCVFSLESMAFLSRLRPISSHRCRFFSSILSPDSATPLSSKEKSRAALSLLKSEQDPQRILEICRAAALTPESHLDRVAFSVAISKLADSKHFDCIRHFLDELKARPDLRTERFVSHAIVLFGQAGMLNDAVRTFEHMHQLGVDRTVRSLNALLFSCILAKNYKEANRIFLEFPKTYGIELNLDSYNTVLKAFSESGSSSSAYSILAEMGRKGVKPNATSFGILLAGFYNEEKYEDVGKVLKMMEEYKMQPGISTYNIRIQSLCKLKKSSEAKALLYGILARRMKPNSETYCHLIHGFCKEGNLDEAKKLFKDMVNRGCKPDSDCYFTLVYFLCQGGDFESALRFCKECMEKGWFPNISTMTSLVNGLVSISKVEEARELIGQIKEKFSRNVDKWNEIEAGLPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DN79</accession>
    <name>A0AA39DN79_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_013146</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DN79"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.330:FF:000001">
        <property type="entry name" value="Protein SRG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050295">
        <property type="entry name" value="Plant_2OG-oxidoreductases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47991">
        <property type="entry name" value="OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="6">
        <location>
            <begin position="205"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9690851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="362" mass="40871" checksum="2F56C892A4FB1B45" modified="2024-01-24" version="1">MAESSSFSSVPPILSVQELAKEPMTAVPQPFILDDPELPLDISNRASLPTIPTIDMKHLIMSETTDFELENLHSTCREWGAFQLVNHGVSSSLVEKLKSEIGEFYRLPLEEKMKYKIRPGSVEGYGLSLIRSQDQKLDWGDRFYMITNPFHRRNPHLLSELPPSLRDTLESYLSEMQKLAMTLLGFMAKALNLDKRDMEELFDDGMQSVRITYYPPCPQPELVMGLTPHSDASGITILLQVNGVDGLQVKKDGVWIPVNFLPDAFVVNLGDILEIVSNGVYNSIEHRAMANSVTERISIAMFFNTKFSAEIGPAIGLINPQNPPLFKRVGMEKYFRDFFARKLEGKAYLEYMKIKNGEGDTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DQJ9</accession>
    <name>A0AA39DQJ9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">PVL29_012236</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="23">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9693383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DQJ9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000129">
        <property type="entry name" value="Leucine-rich repeat receptor-like kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000221">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase PXL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001347">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000416">
        <property type="entry name" value="Probable leucine-rich repeat receptor-like protein kinase At5g63930"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001646">
        <property type="entry name" value="Putative inactive leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000213">
        <property type="entry name" value="Tyrosine-sulfated glycopeptide receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046956">
        <property type="entry name" value="RLP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063">
        <property type="entry name" value="LRR RECEPTOR-LIKE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063:SF16">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="14 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 20" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041383605" description="non-specific serine/threonine protein kinase" evidence="20">
        <location>
            <begin position="23"/>
            <end position="1066"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1009"/>
            <end position="1029"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="21">
        <location>
            <begin position="34"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="22">
        <location>
            <begin position="107"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="22">
        <location>
            <begin position="369"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAJ9693383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1066" mass="119009" checksum="9FD0BE5E774C7322" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DQP2</accession>
    <name>A0AA39DQP2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">O-fucosyltransferase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_011621</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycan metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase GT106 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DQP2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006004">
        <property type="term" value="P:fucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11299">
        <property type="entry name" value="O-FucT_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024709">
        <property type="entry name" value="FucosylTrfase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019378">
        <property type="entry name" value="GDP-Fuc_O-FucTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31741:SF2">
        <property type="entry name" value="O-FUCOSYLTRANSFERASE 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31741">
        <property type="entry name" value="OS02G0726500 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10250">
        <property type="entry name" value="O-FucT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009360">
        <property type="entry name" value="UCP009360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="11" id="KW-0294">Fucose metabolism</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9692634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="440" mass="49515" checksum="E5EC5A6EB20600C2" modified="2024-01-24" version="1">MTAFTVKPLFSIVVLTLTLVVATFLISSPSPFSQFSLSPTSPSRKFDIWSVRRLVEWRPCKWWLDGDLTALPAKNNGYIRVDCYGGLNQMRRDFCDGVGIARLLNATLVLPKFEVAAYWNESSGFADVFDVDYFIQQMDGYVKVVKDLPPEIASREPFRVDTSKRKGQFDYIESVLPSLLEHHYISITPAMSQRRDRYPLYAKAALCQACYNALRLTKVLEEKGAELLQAIPKPFLSLHLRFEPDMVAYSQCQYPSLSPASLESVDAVRGDRKPWTGEMATIWRNRGKCPLTPSETAFILQALSLPTNTNIYLAAGDGLMEIEGLTSIYTNVFTKSSLLSGEDFTSMHGNTKAALDYYVSINSDSYIATYFGNMDKMVAAMRAFNRMSKTVFLSRRAFAEFTSQGLKGKELMEALWKAHADDFGTGRGYALPDCFCEFKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DRV9</accession>
    <name>A0AA39DRV9_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_010295</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DRV9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103511">
        <property type="entry name" value="Chlorophyll a-b binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9694748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="121" mass="13019" checksum="BD73527B3DD67E07" modified="2024-01-24" version="1">MATSSPISALSVLPRSAPTVSLLNQQLSPFNHRSPCSTISQPKKKVSFRVQAAKLPPGVELPKVEPKFEAPFLGFTRTAEIWNSRACMIGLVGIFIVELILNKGILQVIGVDVGKGLDLPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DSU5</accession>
    <name>A0AA39DSU5_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_011854</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DSU5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045344">
        <property type="entry name" value="C-JID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058546">
        <property type="entry name" value="RPS4B/Roq1-like_LRR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF1">
        <property type="entry name" value="RAS SUPPRESSOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20160">
        <property type="entry name" value="C-JID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23286">
        <property type="entry name" value="LRR_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance protein RPS4B/Roq1-like leucine-rich repeats" evidence="5">
        <location>
            <begin position="63"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="C-JID" evidence="4">
        <location>
            <begin position="278"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF20160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9692932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="407" mass="46097" checksum="762EB79FE1BFB133" modified="2024-01-24" version="1">MEKAILNGGFHLSSLEVLSIGNFNLMERGIPSDIFHQSSLETLSLHNCNLMEEGIPSDIWNLSSLVELSLCNCNLTEEEILNHICHLSSFEELSLDGNHFSSIPVGINQLSNLRVLNLSHCKNLLQFPDLPTSLTGLNLNHCKKLQEIPEFPSSLRVLGAHCSDGILFSPSLLSIYPLVNCFKSELIQETEISIGASDFQGKGMSIVIPRSSGILAGIRNGSTGSDEVMTCRDLLYAVFMFWYLMLSLTMDHWTSIEFECGSYWCKVITIFIPGNNGIREWIRQQKNGSQITIELPTDWYQNNDSLGFALYSVYVPLHIECKEDPRSLECELNLHGHRYESLDGLSSELWPMDELSFRSNYLCCHNGGESNRVWVAYYPKVAIKNQCWSNEWRHLKASFQVTWVVNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DTQ5</accession>
    <name>A0AA39DTQ5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tr-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_010986</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DTQ5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010467">
        <property type="term" value="P:gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009409">
        <property type="term" value="P:response to cold"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16263">
        <property type="entry name" value="BipA_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03710">
        <property type="entry name" value="BipA_TypA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03691">
        <property type="entry name" value="BipA_TypA_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01891">
        <property type="entry name" value="TypA_BipA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000016">
        <property type="entry name" value="GTP-binding protein TypA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.250:FF:000001">
        <property type="entry name" value="GTP-binding protein TypA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.240:FF:000002">
        <property type="entry name" value="GTP-binding protein TypA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.870:FF:000003">
        <property type="entry name" value="GTP-binding protein TypA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000055">
        <property type="entry name" value="GTP-binding protein TypA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.250">
        <property type="entry name" value="bipa protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.870">
        <property type="entry name" value="Elongation Factor G (Translational Gtpase), domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006298">
        <property type="entry name" value="BipA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048876">
        <property type="entry name" value="BipA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047041">
        <property type="entry name" value="BipA_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047042">
        <property type="entry name" value="BipA_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047043">
        <property type="entry name" value="BipA_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035651">
        <property type="entry name" value="BipA_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042116">
        <property type="entry name" value="TypA/BipA_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01394">
        <property type="entry name" value="TypA_BipA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908:SF8">
        <property type="entry name" value="TR-TYPE G DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908">
        <property type="entry name" value="TRANSLATION ELONGATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21018">
        <property type="entry name" value="BipA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="1">
        <location>
            <begin position="81"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9695784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="676" mass="75191" checksum="16BB7BEDEB819752" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DWW2</accession>
    <name>A0AA39DWW2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Scarecrow-like protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_007905</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the GRAS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9699054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DWW2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005202">
        <property type="entry name" value="TF_GRAS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31636">
        <property type="entry name" value="OSJNBA0084A10.13 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03514">
        <property type="entry name" value="GRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50985">
        <property type="entry name" value="GRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="VHIID" evidence="3">
        <location>
            <begin position="125"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Leucine repeat II (LRII)" evidence="3">
        <location>
            <begin position="200"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="SAW" evidence="3">
        <location>
            <begin position="394"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="VHIID" evidence="3">
        <location>
            <begin position="156"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="LXXLL motif" evidence="3">
        <location>
            <begin position="249"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9699054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="472" mass="53243" checksum="4E15BC301E1FE7BE" modified="2024-01-24" version="1">MAPMDERSSSATSSPLQLFSMMSPSPSLGSPYPWLRELKSEERGLYLIHLLLTCANHVASGSLENVNIALEQISELASADGDTMQRIAAYFTEALADRILKTWSGLHKALNSTRISFPSEDILVRKLFFELFPFLKMAYVITNHTIIEAMEGEKMVHIIDLNSAEPAQWIALLQALSARPEGPPHLRITGIHPQKEVLEQMAHRLTEEAEKLDIPFQFNPIVSKLENLDFEKLRVKTGEALAISSVLQLHSLLAYDDEFLRKKSPLASKNSNGVHLQRVLQLNQTTLGELLENDVVNRYSLSPDSASSSPPSFTGSPKMDGFLNALWGLTPKLMVITEQDSNHNGSTLMERLLESLYFYAALFDCLESTLPRTSLERLKVEKMLFGEEIKNIIACEGVERKERHEKLEKWIQRLDSAGFQSMPLSYYCMLQAKRLLQGYGCDGYRIKEENGCAVICCQDRPLFSVSAWRCRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DXQ0</accession>
    <name>A0AA39DXQ0_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_007072</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9697777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DXQ0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044566">
        <property type="entry name" value="RMV1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45826:SF8">
        <property type="entry name" value="CATIONIC AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45826">
        <property type="entry name" value="POLYAMINE TRANSPORTER PUT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="170"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="195"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9697777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="236" mass="26097" checksum="E0DF279D6DA7FBEB" modified="2024-01-24" version="1">MIPFVLMFFVAIPKVKPRRWISLGEKGVKKDWNLYFNSGTSTSGITSAHWPEKWRIPRSGKWLMIWIEIGAVLRTIGLFEAQLSSCLYQLVGMAEIGLLPGFLGGDRGGPMGGHPGLNCHNNWSFIHGLHQHHILCELSLQFGNVAGGCLIPVAEMEAARAEEAVPVPMGLPFLIIMSIISCGFFTLMLAIATKLVLLVSSGLTLFGIGCYFLMKFLKKKKWFKFNNVTDDGVVRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DXT9</accession>
    <name>A0AA39DXT9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">AP-2 complex subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_008136</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Subunit of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The complex binds polyphosphoinositides.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Adaptor protein complex 2 (AP-2) is a heterotetramer composed of two large adaptins (alpha-type and beta-type subunits), a medium adaptin (mu-type subunit) and a small adaptin (sigma-type subunit).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <location evidence="1">Coated pit</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the adaptor complexes large subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9699400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DXT9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030122">
        <property type="term" value="C:AP-2 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035615">
        <property type="term" value="F:clathrin adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000020">
        <property type="entry name" value="AP-2 complex subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.10">
        <property type="entry name" value="TATA-Binding Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050840">
        <property type="entry name" value="Adaptor_Complx_Large_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017104">
        <property type="entry name" value="AP2_complex_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003164">
        <property type="entry name" value="Clathrin_a-adaptin_app_sub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008152">
        <property type="entry name" value="Clathrin_a/b/g-adaptin_app_Ig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013041">
        <property type="entry name" value="Clathrin_app_Ig-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009028">
        <property type="entry name" value="Coatomer/calthrin_app_sub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012295">
        <property type="entry name" value="TBP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22780">
        <property type="entry name" value="ADAPTIN, ALPHA/GAMMA/EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02296">
        <property type="entry name" value="Alpha_adaptin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02883">
        <property type="entry name" value="Alpha_adaptinC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037091">
        <property type="entry name" value="AP2_complex_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00809">
        <property type="entry name" value="Alpha_adaptinC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49348">
        <property type="entry name" value="Clathrin adaptor appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55711">
        <property type="entry name" value="Subdomain of clathrin and coatomer appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0168">Coated pit</keyword>
    <keyword evidence="3 8" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Clathrin adaptor alpha/beta/gamma-adaptin appendage Ig-like subdomain" evidence="11">
        <location>
            <begin position="751"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="698"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="7"/>
            <end position="8"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:57836"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:57836"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="53"/>
            <end position="57"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:57836"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00054328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR037091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR037091-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9699400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1016" mass="113039" checksum="A671238213BA55F3" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DYJ0</accession>
    <name>A0AA39DYJ0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycine-rich cell wall structural protein-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_006191</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DYJ0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053329">
        <property type="entry name" value="Merozoite_Surface_Assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39110:SF5">
        <property type="entry name" value="GLYCINE-RICH PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39110">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041283741" description="Glycine-rich cell wall structural protein-like" evidence="1">
        <location>
            <begin position="27"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9700749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="416" mass="31425" checksum="1575C8BFEAABA49C" modified="2024-01-24" version="1">MMGGAKGVGLIALIICFHVVLVSVGARNVADEEEKFFSHGKGGGFGGGGGFGGGAGGGGGFGGGGGFGGGAGGGGGLGGGAGGGGGLGGGAGGGGGFGGGAGGGGGLGGGHGIGGGAGGGFGKGGGLGGGAGGGFGKGGGVGGGAGGGFGKGGGLGGGHGIGGGAGGGSGKGGGLGGGHGIGGGAGGGFGKGGGLGGGHGIGGGAGGGFGKGGGAGGGIGKGGGIGGGAGGGFGKGGGVGGGAGGGFGKGGGIGGGIGKGGGVGGGAGGGFGKGGGVGGGIGKGGGVGGGAGGGFGKGGGVGGGAGGGFGKGGGIGGGAGGGFGKGGGIGGGAGGGIGKGGGIGGGAGGGFGKGGGGGIGGGGGFGGGIGGGSGGGIGGGFGKGGGVGGGSGGGFGGGFGGGGGGGGGGGFGGGHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E022</accession>
    <name>A0AA39E022_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glycosyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_006912</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E022"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080043">
        <property type="term" value="F:quercetin 3-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080044">
        <property type="term" value="F:quercetin 7-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032787">
        <property type="term" value="P:monocarboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000019">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000057">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926">
        <property type="entry name" value="GLUCOSYL/GLUCURONOSYL TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926:SF1311">
        <property type="entry name" value="UDP-GLYCOSYLTRANSFERASE 74F2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9701740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="458" mass="51349" checksum="2B063DFC3B95ADCF" modified="2024-01-24" version="1">MEKEKKSYGAHILALPYPSQGHINPMLQFSRRLVSKGLKATLATPIFISKTFKPQAGSVQLDTISDGFDEGGFMQAESIHEYLTQLEAAGSRTLAQLIQKHRDLGHPFDCIVYDAILPWALDVAKQFGLVGAAFFTQNCAVNYIYYHAYHGLLPLPVKSTPVSIPGLPLLELRDMPSFIYVAGSYPAYFQLVLNQFCNVHKADWVLVNTFYKLEEEVVDAMAKLSPLITIGPTIPSKYLDNRLENEAEYGFDLFYSEPSAHTINWLDNKPTRSVIYVSFGSMACLSEAQMEELAWGLKGSGHYFLWAVRDSEEAKLPKHFIHETSGKGWFVKWSPQLEVLANDAVGCFFTHCGWNSTVEALSLGVPMVGMLQWTDQTTDAKFVEDVWKVGIRVRVDENGIVGRKEVEDCIREVMEGERGKDMKENAKKWRKLAVEAVSEGGTSDKNIDEFVAKLIISP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E0B7</accession>
    <name>A0AA39E0B7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ENTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_004344</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Clathrin-coated vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <location evidence="3">Clathrin-coated pit</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E0B7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005545">
        <property type="term" value="F:1-phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032050">
        <property type="term" value="F:clathrin heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048268">
        <property type="term" value="P:clathrin coat assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006900">
        <property type="term" value="P:vesicle budding from membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16987">
        <property type="entry name" value="ANTH_N_AP180_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000027">
        <property type="entry name" value="Putative clathrin assembly protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.150">
        <property type="entry name" value="ANTH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011417">
        <property type="entry name" value="ANTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014712">
        <property type="entry name" value="ANTH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048050">
        <property type="entry name" value="ANTH_N_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045192">
        <property type="entry name" value="AP180-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951">
        <property type="entry name" value="CLATHRIN ASSEMBLY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951:SF19">
        <property type="entry name" value="OS08G0467300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07651">
        <property type="entry name" value="ANTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89009">
        <property type="entry name" value="GAT-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0168">Coated pit</keyword>
    <keyword evidence="8" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="4" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="9">
        <location>
            <begin position="25"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9702564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="373" mass="42493" checksum="8CED9C5F77F3C73F" modified="2024-01-24" version="1">MKLWRRASGALKDKNSMLIASLSSRNAPWNPRFEVAIIKATSHDESIVDYENIKRVFAWLHASPAYLKPLLSALSTRLQKTCSWVVALKGLMLMHGVLSCNIPAVQKIGRLPFDLSNFRDSYRNSGRTWGLNTFVQSYFEFLDQTSAFLYMERKEESKETESALVQELVKLQQWQSLLGILLQIRPQAKEMDIAHVYEAMDFVIIEIFSFYNRICNGIARVLTGIYAAEKVEAAMALEILQKASQQREQLALYLNFYRKIGVFNESKFPEVEHIPKMDIQKLELIVSGVSENQAAVVKRIEPKEEQEEPKRVLKTIITETWEVFDEDPDSNGIGISNIPGKTTVGQNPSAAPLKFPPNTHGQKYDFPDLISFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E324</accession>
    <name>A0AA39E324_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PVL29_003047</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E324"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000239">
        <property type="entry name" value="Proline-rich receptor-like protein kinase PERK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000207">
        <property type="entry name" value="proline-rich receptor-like protein kinase PERK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047117">
        <property type="entry name" value="PERK1-13-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47982">
        <property type="entry name" value="PROLINE-RICH RECEPTOR-LIKE PROTEIN KINASE PERK4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47982:SF47">
        <property type="entry name" value="PROLINE-RICH RECEPTOR-LIKE PROTEIN KINASE PERK6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="12 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="168"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="303"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="580"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="622"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="32"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="98"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="589"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="622"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="331"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAJ9704815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="661" mass="70756" checksum="704FCC6F0FA09360" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E3M4</accession>
    <name>A0AA39E3M4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein SUPPRESSOR OF FRI 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_003852</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E3M4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23215:SF0">
        <property type="entry name" value="BUB3-INTERACTING AND GLEBS MOTIF-CONTAINING PROTEIN ZNF207"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23215">
        <property type="entry name" value="ZINC FINGER PROTEIN 207"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="71"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9705939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="246" mass="25904" checksum="A54DA635A2ED4310" modified="2024-01-24" version="1">MPLGSVGMGFPPQSTFGAIPPIYNPAVAVPPDWPVRRPQPWFPQHPAVPVPPAAPLGLAQRPLFPIQNVTPPLPSATLQPSFQTTPPGPLSSSPTPVSQPLFPVVGTASIPSQNSQVTAAALSAPITSSTPSELNNPSYSSANTSMVNGYHAPSIQAGTLVSSHTYASGPNTSGPSIGPPPMIANKAPATQPASNEVYLVWDDEAMSMEERRMSLLKYQVHDETSQVSYDCLVILFIQLGMCGIKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E3Y9</accession>
    <name>A0AA39E3Y9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Magnesium-dependent phosphatase 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_003711</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E3Y9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07501">
        <property type="entry name" value="HAD_MDP-1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000120">
        <property type="entry name" value="Magnesium-dependent phosphatase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010033">
        <property type="entry name" value="HAD_SF_ppase_IIIC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035679">
        <property type="entry name" value="MDP-1_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010036">
        <property type="entry name" value="MDP_1_eu_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01681">
        <property type="entry name" value="HAD-SF-IIIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17901:SF14">
        <property type="entry name" value="MAGNESIUM-DEPENDENT PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17901">
        <property type="entry name" value="MAGNESIUM-DEPENDENT PHOSPHATASE 1 MDP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12689">
        <property type="entry name" value="Acid_PPase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01131">
        <property type="entry name" value="C1.5.2:_MDP_Like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9705745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="173" mass="19536" checksum="A11DF6D88CF348FD" modified="2024-01-24" version="1">MAESRVKEEALQIIGLCQVLPRLVVFDLDYTLWPFYCECRSKREMPSLYPHAEGILYALKEKGIDMAIASRSPTPDIAKTFLDKLGIKSMFVAQEIFSSWSHKTEHFQRIHRRTGVPFNSMLFFDDEDRNIESVSKMGATSILVGNGLNIGALRQGLTKFSQNSASSGNTKRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E5Q5</accession>
    <name>A0AA39E5Q5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_001061</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E5Q5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF347">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9709403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="250" mass="27342" checksum="B8082277649D09A4" modified="2024-01-24" version="1">MSNSAECCIACSKNPMSPASGSIAPFDIGCPPIISSFNDPIRNRGNDAFGLVFSTIHAYGRSLPLKMATTISRQNEELPRKFSGNQLHAYSIRTGPKAYTHLVPRGQIGYACHLFHQTPWMIPVVWNAIITGCVENKPIEIALNLFPEMHQLGVRHDKYAFASVPSLCSPELLDFGREVHTLVIKTGLSVRASVINALLTMYFKSGKVADAYEVFEEVESTVHDDITINVMIADLAKGHLPGQNGICPST</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YGS4</accession>
    <name>A0AA38YGS4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">LMBR1-like membrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_026612</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LIMR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YGS4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051584">
        <property type="entry name" value="GPCR-associated_LMBR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006876">
        <property type="entry name" value="LMBR1-like_membr_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21355">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR-ASSOCIATED PROTEIN LMBRD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21355:SF0">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR-ASSOCIATED PROTEIN LMBRD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04791">
        <property type="entry name" value="LMBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="111"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="140"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="345"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="385"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="478"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="712"/>
            <end position="736"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9670171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="736" mass="83106" checksum="89BCB8B5DBD244BF" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YGV7</accession>
    <name>A0AA38YGV7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PVL29_026624</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the RING-type zinc finger family. ATL subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YGV7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16461">
        <property type="entry name" value="RING-H2_EL5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000187">
        <property type="entry name" value="E3 ubiquitin-protein ligase ATL6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053238">
        <property type="entry name" value="RING-H2_zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14155:SF263">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE ATL6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14155">
        <property type="entry name" value="RING FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="64"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="17">
        <location>
            <begin position="141"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="195"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="244"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="298"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="199"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="319"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ9670190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="353" mass="39262" checksum="B9A9D9ABA07DF611" modified="2024-01-24" version="1">MRKKLNWSRLIVRQLCDFLHAVPYGITYFKLMPLLPLVLPSATAQTGTMSPNNPYGIDANFNPSMTIIIVVLVLAFFFMGLLSVYIRQCTESRITGSERPATAINIGNSTQGLDVALIQAFPVFVYSSVKDLKIGKGTLECAVCLSEFEDEETLRLLPKCDHVFHPNCIDAWLASHTTCPVCRANLTLDSGELSGRMAESPYSSDSSDEPTSTSELTELTQVTIDVDEEQTRDSRVLLMVNTRESPQAKFPRSHSTGHLPVQPGGSSERHTLRLPEEVRKQILSAKLRRTSSSVEALQRQWSSRRGYRSGGEGSSRGRSNVDRQVGRSERTARSDRWVFSKAPPFFSRTVSGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YHB1</accession>
    <name>A0AA38YHB1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein PHLOEM PROTEIN 2-LIKE A10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_026775</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YHB1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019141">
        <property type="entry name" value="DUF2045"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21477:SF12">
        <property type="entry name" value="PROTEIN PHLOEM PROTEIN 2-LIKE A10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21477">
        <property type="entry name" value="ZGC:172139"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9670444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="385" mass="41735" checksum="5A64F8DA9CD91D9F" modified="2024-01-24" version="1">MDLQLLNEGLDFFQKRKLAVVLAAFGFTSYGVYRVYQSPSLVDKRNKLCKLLEALASIVEASSDSAQTVGLVSRDLKAFLESDSDQIPKSLKQLSKIMSSKEFSESMTGVTQAITVGLLNGYGSTSNEMSDVSSFLDRVVDKLLTPAGSGFASAVVGSFAKNMVMALFSDGQSCVDDTGSQATCIPGLVNVICSEKCKELISDCIQLFVSTAVAVYLEKTKDINTYDEILSGLTNPKHEKEVRDLLLSVCNGAVETLVKTSYQVLTSSSSGSSYLGKDQEVLYDEIEKGGWLGKLLSTLAVPSYRKFIISMTLTITLETVQSFLELLLEKLYDGLKKSINAVRETLVDGGLQVVRYVSAKCSVVTVCISFCLHILGGAGWPTIFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YI07</accession>
    <name>A0AA38YI07_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">STAS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_026990</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YI07"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008271">
        <property type="term" value="F:secondary active sulfate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07042">
        <property type="entry name" value="STAS_SulP_like_sulfate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.750.24:FF:000002">
        <property type="entry name" value="Sulfate transporter 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="entry name" value="STAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018045">
        <property type="entry name" value="S04_transporter_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011547">
        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001902">
        <property type="entry name" value="SLC26A/SulP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00815">
        <property type="entry name" value="sulP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11814">
        <property type="entry name" value="SULFATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01740">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00916">
        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SpoIIaa-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01130">
        <property type="entry name" value="SLC26A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="153"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="221"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="276"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="301"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="395"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="425"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="450"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="485"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="domain" description="STAS" evidence="7">
        <location>
            <begin position="563"/>
            <end position="666"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9670788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="675" mass="73508" checksum="1ED1CB43B767752B" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YIS9</accession>
    <name>A0AA38YIS9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Histone deacetylation protein Rxt3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_027295</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9671235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YIS9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.130.20">
        <property type="entry name" value="LCCL-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036609">
        <property type="entry name" value="LCCL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013951">
        <property type="entry name" value="Rxt3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08642">
        <property type="entry name" value="Rxt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69848">
        <property type="entry name" value="LCCL domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="425"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="708"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="20"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="48"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="219"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="255"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="272"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="282"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="353"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="709"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="720"/>
            <end position="730"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9671235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="806" mass="90245" checksum="F7B93EE487E0C26F" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YJX0</accession>
    <name>A0AA38YJX0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_025485</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YJX0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048544">
        <property type="term" value="P:recognition of pollen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01098">
        <property type="entry name" value="PAN_AP_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000060">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000951">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000858">
        <property type="entry name" value="S_locus_glycoprot_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002">
        <property type="entry name" value="RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD1-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002:SF1075">
        <property type="entry name" value="RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08276">
        <property type="entry name" value="PAN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00954">
        <property type="entry name" value="S_locus_glycop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00473">
        <property type="entry name" value="PAN_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57414">
        <property type="entry name" value="Hairpin loop containing domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="279"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="15">
        <location>
            <begin position="181"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="356"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="384"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9671793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="671" mass="76731" checksum="7ED270543A1C0B6D" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YLG1</accession>
    <name>A0AA38YLG1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Retrovirus-related Pol polyprotein from transposon TNT 1-94</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_026022</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YLG1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="8"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9672628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="73" mass="7974" checksum="F6DC41E81B8E86F1" modified="2024-01-24" version="1">MGKAKPVSSSLGSHLKLSSKQSPSSEKEKEEMRNVPYASAVDSLMYAMVCKRPDIAHVVEVVSKFLSNPGKEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YMU2</accession>
    <name>A0AA38YMU2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_023109</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May act as a substrate-specific adapter of an E3 ubiquitin-protein ligase complex (CUL3-RBX1-BTB) which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YMU2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18186">
        <property type="entry name" value="BTB_POZ_ZBTB_KLHL-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000421">
        <property type="entry name" value="FA58C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022041">
        <property type="entry name" value="Methyltransf_FA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47457:SF1">
        <property type="entry name" value="BTB DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47457">
        <property type="entry name" value="OS05G0345500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00754">
        <property type="entry name" value="F5_F8_type_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12248">
        <property type="entry name" value="Methyltransf_FA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="208"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="348"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9673325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="806" mass="92190" checksum="1106000B0F36CA60" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YS87</accession>
    <name>A0AA38YS87_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Exocyst complex component SEC5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_024484</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the exocyst complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the SEC5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YS87"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029175">
        <property type="entry name" value="EXOC2/Sec5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039481">
        <property type="entry name" value="EXOC2/Sec5_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13043:SF1">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13043">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT SEC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15469">
        <property type="entry name" value="Sec5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex component EXOC2/Sec5 N-terminal" evidence="6">
        <location>
            <begin position="181"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="19"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1070"/>
            <end position="1096"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="110"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1073"/>
            <end position="1096"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU365069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF15469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9675582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1096" mass="122993" checksum="79A9B6F86975FC63" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YSK1</accession>
    <name>A0AA38YSK1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase</fullName>
            <ecNumber evidence="1">3.2.2.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_024611</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>NAD(+) + H2O = ADP-D-ribose + nicotinamide + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16301"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57967"/>
            <dbReference type="EC" id="3.2.2.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:16302"/>
        </physiologicalReaction>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.6" evidence="1"/>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YSK1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061809">
        <property type="term" value="F:NAD+ nucleosidase activity, cyclic ADP-ribose generating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32009:SF39">
        <property type="entry name" value="TIR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32009">
        <property type="entry name" value="TMV RESISTANCE PROTEIN N-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TIR" evidence="5">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9675763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="103" mass="12033" checksum="891BD54FFF7C8A34" modified="2024-01-24" version="1">MGEDTRHNFTDHLYRTLNRKGIRTFRDDEELPRREEIAAELLKAIEESRICLIILSENYARSRWCLEELTKIMDCRGANGKISFSDFLPRGTSAWWRVSPDGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YTL2</accession>
    <name>A0AA38YTL2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">(S)-ureidoglycine aminohydrolase cupin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_025122</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9676440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YTL2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02227">
        <property type="entry name" value="cupin_TM1112-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008579">
        <property type="entry name" value="UGlyAH_Cupin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33271">
        <property type="entry name" value="OS04G0445200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33271:SF1">
        <property type="entry name" value="RMLC-LIKE JELLY ROLL PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05899">
        <property type="entry name" value="Cupin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="(S)-ureidoglycine aminohydrolase cupin" evidence="1">
        <location>
            <begin position="63"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9676440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="139" mass="15286" checksum="326152A0F8ABCD34" modified="2024-01-24" version="1">MPSKFTKLASSNAAAVVILLLSVALVVITSNMTKPNSQEASTAITEIFGVKIHRNPPQATLTDLGVTSWKKWGCSPSKFPWTFEAKETMYLLEGKVKVYCDGHDGFFEIGAGDLVEFPKGMKVTWDVTEALSKHYSLEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YUP1</accession>
    <name>A0AA38YUP1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">S-adenosylmethionine-dependent methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_022064</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9676873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YUP1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.270">
        <property type="entry name" value="Methyltransferase, alpha-helical capping domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005299">
        <property type="entry name" value="MeTrfase_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042086">
        <property type="entry name" value="MeTrfase_capping"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31009">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE:CARBOXYL METHYLTRANSFERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03492">
        <property type="entry name" value="Methyltransf_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9676873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="376" mass="42299" checksum="6B2B068687181062" modified="2024-01-24" version="1">MASEKEMRNIGEAHPMKSGDGLYSYSNNSYLQRDIINAAKQIVGEAIVESLDILKFSPSTTIRVADLGCSVGPNTFFAMENILEAIELKCQNQGLHSQIPEFQVFFNDHTSNDFNSLFSVLPPNRRYHSAGVPGSFYSRLFPNHSLHIVYSSFSIQWLSRVPKKVEDMSSPAWNKGRIYYVSAADEVVEAYSAQCAEDTARFLQARAQEIADGGLMILIFPARPDGIPHSQFGGNILFDMLGFCLMDMAKKARNMGIVSEEKVDMFNLPVYHMSDQELEAAVERNRCFSIERMERLRPISSTLQSLVSTRDRAQAISFHVRAAMEGLIKAHFDEEILDQLFDSFSKKLEQEYSLIESAGRSAPNLCAVLKRKTMNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YW92</accession>
    <name>A0AA38YW92_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protease Do-like 9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_022576</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S1C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YW92"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000012">
        <property type="entry name" value="protease Do-like 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000131">
        <property type="entry name" value="Protease Do-like 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041517">
        <property type="entry name" value="DEGP_PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046449">
        <property type="entry name" value="DEGP_PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000637">
        <property type="entry name" value="HMGI/Y_DNA-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001940">
        <property type="entry name" value="Peptidase_S1C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45980">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45980:SF19">
        <property type="entry name" value="PEPTIDASE DO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13180">
        <property type="entry name" value="PDZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17815">
        <property type="entry name" value="PDZ_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13365">
        <property type="entry name" value="Trypsin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00834">
        <property type="entry name" value="PROTEASES2C"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00354">
        <property type="entry name" value="HMGI_Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="PDZ" evidence="8">
        <location>
            <begin position="336"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="domain" description="Protease Do-like PDZ" evidence="9">
        <location>
            <begin position="427"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="39"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF17815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9677667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="575" mass="63634" checksum="3A2CCE38EFAD2EE8" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YW94</accession>
    <name>A0AA38YW94_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF7792 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_022688</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YW94"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.20">
        <property type="entry name" value="Adaptor protein Cbl, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036537">
        <property type="entry name" value="Adaptor_Cbl_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056694">
        <property type="entry name" value="DUF7792"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46168">
        <property type="entry name" value="ARMADILLO REPEAT ONLY 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46168:SF15">
        <property type="entry name" value="ARMADILLO REPEAT-CONTAINING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25055">
        <property type="entry name" value="DUF7792"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="DUF7792" evidence="5">
        <location>
            <begin position="10"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="2">
        <location>
            <begin position="191"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="342"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="30"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="342"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9677856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="618" mass="67546" checksum="76304DDD537E1BA6" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YWI3</accession>
    <name>A0AA38YWI3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Zinc finger PMZ-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_022712</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YWI3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018289">
        <property type="entry name" value="MULE_transposase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004332">
        <property type="entry name" value="Transposase_MuDR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006564">
        <property type="entry name" value="Znf_PMZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31973:SF195">
        <property type="entry name" value="MUDR FAMILY TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31973">
        <property type="entry name" value="POLYPROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03108">
        <property type="entry name" value="DBD_Tnp_Mut"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10551">
        <property type="entry name" value="MULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04434">
        <property type="entry name" value="SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00575">
        <property type="entry name" value="ZnF_PMZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Zinc finger PMZ-type" evidence="4">
        <location>
            <begin position="718"/>
            <end position="745"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9677900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="842" mass="97184" checksum="A308606716173FA7" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YXM2</accession>
    <name>A0AA38YXM2_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_020468</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YXM2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF78">
        <property type="entry name" value="OS12G0587133 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9678314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="251" mass="29049" checksum="9F0E694A452A2EDD" modified="2024-01-24" version="1">MEALGSLIAKAVQRRFIRGFKIEGRGEIENQVSHLLFVDDTLLFCEDDEEQLNFWKYIIMCFELVSGLKINVQKSEVIPVVGTMDVNRATSLFGCKVGKLPTSYLGLPFGAPNKHCGVWDSIKERFKRKLAVWKKHCLSKGGRLVLLKSTISNLPIYFMSLFVILRKVRIRLERIQRDFLWGDLGDKSKIHLVKWSNVCKAKNFGGLGIRRLYSLNQALLGKCLWRFFVERESLWRKIICESSGRWRGLDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YYS4</accession>
    <name>A0AA38YYS4_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_021185</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YYS4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009908">
        <property type="term" value="P:flower development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099402">
        <property type="term" value="P:plant organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00265">
        <property type="entry name" value="MADS_MEF2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1810.10:FF:000030">
        <property type="entry name" value="Agamous-like MADS-box protein AGL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050142">
        <property type="entry name" value="MADS-box/MEF2_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033896">
        <property type="entry name" value="MEF2-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002487">
        <property type="entry name" value="TF_Kbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48019">
        <property type="entry name" value="SERUM RESPONSE FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01486">
        <property type="entry name" value="K-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00404">
        <property type="entry name" value="MADSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51297">
        <property type="entry name" value="K_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00350">
        <property type="entry name" value="MADS_BOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="7">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="K-box" evidence="8">
        <location>
            <begin position="61"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="117"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9679176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="184" mass="21107" checksum="D3B5CE4111704412" modified="2024-01-24" version="1">MVRGKIQMRRIENATSRQVTFSKRRNGLLKKAYELSVLCDAEVAVIIFSQKGRLYEFSSSKYNLKQDTESMAKKIELLEVSQRKLLGQGLSSCSLDEILEIDGQLEKSLKNIRARKAQIFQEQIEQLKEREKQLLEENARLSQKDTRQWQLSAQPSEGVTYSQSSPSSEVVTELFIGLPEMRCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YZY4</accession>
    <name>A0AA38YZY4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PVL29_021664</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the RING-type zinc finger family. ATL subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YZY4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16461">
        <property type="entry name" value="RING-H2_EL5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046948">
        <property type="entry name" value="ATL20-22-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025287">
        <property type="entry name" value="WAK_GUB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46279:SF10">
        <property type="entry name" value="RING-TYPE E3 UBIQUITIN TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46279">
        <property type="entry name" value="RING/U-BOX SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13947">
        <property type="entry name" value="GUB_WAK_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 17" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041296763" description="RING-type E3 ubiquitin transferase" evidence="17">
        <location>
            <begin position="35"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="242"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="18">
        <location>
            <begin position="322"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00024209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ9679818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="376" mass="41729" checksum="E646EEA390BC9D12" modified="2024-01-24" version="1">MVKILRRLDLIKMDTIPFFFSIFLLSTMAANSQACEKSSCQRGEPEIRFPFRLEDHQPESCGYPGFDLSCDATMQTILKLPSGEFSVQGIDYGTQEIWINDPKNCLPGLILSLNLSGSSFSGIYYHNYSFFKCSAGSKYPLDSIACLSDDNYTVFATNSSRAINFLSPVCDLIKSVMVPVELPFYDPVLTSDLSSDLRLSWGAPQCGDCEMEGGRCGFRSNSSLEIGCFDVPKRGLPRTARYAITVGVGIPALMCFIGLLCFLCGRVRAFGVRRRRPIAEFTSMVTPQPTAVMGLDGPTIESYPKVVLGESRRLPKPDDNTCSICLSEYRPKETLKIIPECQHCFHAECIDEWLHLNASCPICRNSPEKSPPSQES</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z084</accession>
    <name>A0AA38Z084_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Metal-nicotianamine transporter YSL3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_021761</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the YSL (TC 2.A.67.2) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z084"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051980">
        <property type="term" value="F:iron-nicotianamine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035673">
        <property type="term" value="F:oligopeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010039">
        <property type="term" value="P:response to iron ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048316">
        <property type="term" value="P:seed development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004813">
        <property type="entry name" value="OPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045035">
        <property type="entry name" value="YSL-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00728">
        <property type="entry name" value="OPT_sfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31645:SF4">
        <property type="entry name" value="METAL-NICOTIANAMINE TRANSPORTER YSL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31645">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER YGL114W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03169">
        <property type="entry name" value="OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="70"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="110"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="155"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="216"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="265"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="320"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="381"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="410"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="443"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="482"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="538"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="609"/>
            <end position="629"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9679965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="650" mass="71223" checksum="644B066B9042E26E" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z0K9</accession>
    <name>A0AA38Z0K9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_021763</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AAA ATPase family. BCS1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z0K9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025753">
        <property type="entry name" value="AAA_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058017">
        <property type="entry name" value="At3g28540-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050747">
        <property type="entry name" value="Mitochondrial_chaperone_BCS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23070">
        <property type="entry name" value="BCS1 AAA-TYPE ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14363">
        <property type="entry name" value="AAA_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25568">
        <property type="entry name" value="AAA_lid_At3g28540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="8">
        <location>
            <begin position="217"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="409"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="453"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="409"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9679967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="472" mass="53559" checksum="C7CCB6F53FCF3513" modified="2024-01-24" version="1">MILSDSIPFLLAFAATFLILRLLLKTSVILIIRKWVRSLGDMCHVWQCYRVPQYNQLLQENELYRKISAYINSLASVEDSDFANLVTGSRSNDVVLSLDPNQTVFDSFLGARVAWTNVVGESDGRRCFVLRIRKKDKRRILRPYLQHILAKCEEFERELKLYINCESRDGRWRSVPFAHQATMETVAMDSELKSKVKSDLELFLKSKQYYHRLGRVWKRSYLLHGASGTGKSSFVAAMAKLLCYDVYDVDLSQVSDDADLKLLLLQTTPRSLILIEDLDRFLKDKSTTVSLPGLLNFMDGVLSCCGEERVMVFTMNNPDQIDPTVLRPGRIDVHVQFGLCDFSSFKMLANSHLAIKEHRLFPQVEEIFQTGASLSPAEIGEIMISNRNSATRALKSVINALQTNTANKIRLTQSSSGRSTDESAEPGGVICRESVHTVREFRKLYGLLRRSGRKDEPLDLGSTDKEAPRITQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z0V4</accession>
    <name>A0AA38Z0V4_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_021732</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the peptidase S8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z0V4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02120">
        <property type="entry name" value="PA_subtilisin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04852">
        <property type="entry name" value="Peptidases_S8_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.2310:FF:000002">
        <property type="entry name" value="p69E protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000006">
        <property type="entry name" value="Subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000005">
        <property type="entry name" value="subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.80:FF:000002">
        <property type="entry name" value="Subtilisin-like protease SBT5.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034197">
        <property type="entry name" value="Peptidases_S8_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010259">
        <property type="entry name" value="S8pro/Inhibitor_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045051">
        <property type="entry name" value="SBT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041469">
        <property type="entry name" value="Subtilisin-like_FN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10795">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17766">
        <property type="entry name" value="fn3_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05922">
        <property type="entry name" value="Inhibitor_I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 7" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041358180" evidence="8">
        <location>
            <begin position="28"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="domain" description="Inhibitor I9" evidence="10">
        <location>
            <begin position="37"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="9">
        <location>
            <begin position="142"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="domain" description="Subtilisin-like protease fibronectin type-III" evidence="11">
        <location>
            <begin position="669"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6 7">
        <location>
            <position position="151"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6 7">
        <location>
            <position position="228"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6 7">
        <location>
            <position position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9679919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="776" mass="82487" checksum="896D0CF6FBFFD881" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z131</accession>
    <name>A0AA38Z131_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_019460</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z131"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009791">
        <property type="term" value="P:post-embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000233">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase TDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000548">
        <property type="entry name" value="Receptor-like protein kinase HSL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052592">
        <property type="entry name" value="LRR-RLK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48054:SF21">
        <property type="entry name" value="KINASE FAMILY WITH LEUCINE-RICH REPEAT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48054">
        <property type="entry name" value="RECEPTOR KINASE-LIKE PROTEIN XA21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041384022" description="Leucine-rich repeat-containing N-terminal plant-type domain-containing protein" evidence="12">
        <location>
            <begin position="30"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="569"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="13">
        <location>
            <begin position="33"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="14">
        <location>
            <begin position="114"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="666"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="685"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="655"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9680172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="727" mass="80478" checksum="DE8BC710E790CBBB" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z142</accession>
    <name>A0AA38Z142_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_019547</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z142"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9680267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="97" mass="11278" checksum="5C0E9E9DC39CDE09" modified="2024-01-24" version="1">MDLRNIDVRIDDENQAIILMHSLPNSYEHFVDTMMHARDTLSIKDVRVALNSKELKKRSKSKKGDSSEGLVARRRIGKKNNNRKSRSRSKSKLRENN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z1Y3</accession>
    <name>A0AA38Z1Y3_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_020020</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z1Y3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37218">
        <property type="entry name" value="COILED-COIL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37218:SF2">
        <property type="entry name" value="COILED-COIL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9680886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="242" mass="27370" checksum="18C58927D88451A0" modified="2024-01-24" version="1">MGGKGRRRREKNYRAAHGGYERLPPPPDLSKLDALPSKLRKLMAFTSPSAPRPPQGSAKASIDKGKKGKRGDAEEKKLHLKDELNYKTTKIDAAGAGEHFMTSQDMEDNDGTVPSSTHEKGKRKRKRKQVKDLRFEAELEKVAGLGSKRRERKKKYLESRKKKHKKAKTEENLDFPRQEKIKFGEVVEAPPKLVVVPKALKTVQDASQERLRLQAVDAYRKRKGWVSRPGIHLPPPVTTTPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z2G3</accession>
    <name>A0AA38Z2G3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Auxin response factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_020167</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Auxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs).</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimers and heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ARF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9681150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z2G3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10017">
        <property type="entry name" value="B3_DNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.1040:FF:000001">
        <property type="entry name" value="Auxin response factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.330.10:FF:000001">
        <property type="entry name" value="Auxin response factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000047">
        <property type="entry name" value="Auxin response factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.330.10">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010525">
        <property type="entry name" value="ARF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044835">
        <property type="entry name" value="ARF_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033389">
        <property type="entry name" value="AUX/IAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003340">
        <property type="entry name" value="B3_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015300">
        <property type="entry name" value="DNA-bd_pseudobarrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31384:SF8">
        <property type="entry name" value="AUXIN RESPONSE FACTOR 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31384">
        <property type="entry name" value="AUXIN RESPONSE FACTOR 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06507">
        <property type="entry name" value="ARF_AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02309">
        <property type="entry name" value="AUX_IAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02362">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01019">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101936">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50863">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0927">Auxin signaling pathway</keyword>
    <keyword evidence="4 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TF-B3" evidence="10">
        <location>
            <begin position="125"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="PB1" evidence="11">
        <location>
            <begin position="586"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="555"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="555"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="577"/>
            <end position="588"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9681150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="701" mass="77837" checksum="DB8FA423B776E6D1" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z3Y9</accession>
    <name>A0AA38Z3Y9_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_017835</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0057 (PMP3) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9681628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z3Y9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000612">
        <property type="entry name" value="PMP3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21659:SF120">
        <property type="entry name" value="HYDROPHOBIC PROTEIN LTI6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21659">
        <property type="entry name" value="HYDROPHOBIC PROTEIN RCI2 LOW TEMPERATURE AND SALT RESPONSIVE PROTEIN LTI6 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01679">
        <property type="entry name" value="Pmp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01309">
        <property type="entry name" value="UPF0057"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="31"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9681628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="57" mass="6239" checksum="D355F2225BBFECC6" modified="2024-01-24" version="1">MADEGTATCVDILLAIILPPLGVFLKFGCQVEFWICLVLTLFGYLPGIIYAVYAITK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z7F9</accession>
    <name>A0AA38Z7F9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2 NT-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_019038</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z7F9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019448">
        <property type="entry name" value="NT-C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021827">
        <property type="entry name" value="Nup186/Nup192/Nup205"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31344:SF15">
        <property type="entry name" value="EEIG1_EHBP1 PROTEIN AMINO-TERMINAL DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31344">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10358">
        <property type="entry name" value="NT-C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51840">
        <property type="entry name" value="C2_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2 NT-type" evidence="3">
        <location>
            <begin position="7"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="200"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="396"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="683"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1032"/>
            <end position="1081"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="564"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="200"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="219"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="241"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="281"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="345"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="362"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="436"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="710"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1070"/>
            <end position="1081"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9683279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1099" mass="120628" checksum="EBE5ADA712B0EE7F" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z7R0</accession>
    <name>A0AA38Z7R0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein NRT1/ PTR FAMILY 5.1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_016463</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9683976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z7R0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071916">
        <property type="term" value="F:dipeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042937">
        <property type="term" value="F:tripeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17417">
        <property type="entry name" value="MFS_NPF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044739">
        <property type="entry name" value="NRT1/PTR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000109">
        <property type="entry name" value="POT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11654">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00854">
        <property type="entry name" value="PTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="95"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="181"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="209"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="322"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="363"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="404"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="456"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="487"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="532"/>
            <end position="551"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9683976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="585" mass="65101" checksum="56904D755D4D24A7" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z7U2</accession>
    <name>A0AA38Z7U2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Beta-amyrin 28-monooxygenase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Beta-amyrin 28-oxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_016488</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>beta-amyrin + 3 reduced [NADPH--hemoprotein reductase] + 3 O2 = oleanolate + 3 oxidized [NADPH--hemoprotein reductase] + 4 H2O + 4 H(+)</text>
            <dbReference type="Rhea" id="RHEA:43068"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:10352"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:82828"/>
            <dbReference type="EC" id="1.14.14.126"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z7U2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102373">
        <property type="term" value="F:beta-amyrin 28-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016709">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016125">
        <property type="term" value="P:sterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11043">
        <property type="entry name" value="CYP90-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000022">
        <property type="entry name" value="Taxadiene 5-alpha hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286">
        <property type="entry name" value="CYTOCHROME P450 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286:SF256">
        <property type="entry name" value="CYTOCHROME P450 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 13" id="KW-0349">Heme</keyword>
    <keyword evidence="8 12 13" id="KW-0408">Iron</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 12 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="12">
        <location>
            <position position="442"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00052804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00073578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00083450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9684018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="496" mass="55749" checksum="4DD56863DF575224" modified="2024-01-24" version="1">MEVLLSSLEESSFEWKLPLISTILVTLIVLLAGSIKLKFSPPVDKKLPPGSFGFPFIGETISFLRAQRQDKTVEWIESRIAKYGPVFKTSLMGSKVVVLTGQAGNRFLFSGSDNGILSNQPMSVAKILGKHSIFELAGTRHKLVRGAIMEFLKPESIQRSVSRMDSVVQQQLFQELEGKDSVQMVGLMKKITFKVTCSLLFGLPDGKDTEELLEDFTTALKGAWTVPWDLPGTVFRKALQARGRICKQLAQLVRERKAKIEEGRVDSHEDIISSLITLRQENGQPLSEEEIIDNLISVVIASHDTSTVLLGLLIRHLARDTEVCKKVLEEQKQVAKAREGKSNGKLTWGEVQMMKYTWRVAQELMRMTPPVLGNFKCAWRDTSFGGFDIPKGWQVFWVAPGTHMDKKIFEEPEKFDPSRFENPSTSVPPYAYLAFGAGPRACPGADFSRVEVLLMIHNLITKYQWAEMIIDEPIVREPMPYPAMGLPVKLYQRSTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z8V8</accession>
    <name>A0AA38Z8V8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SHSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_016749</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z8V8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd06472">
        <property type="entry name" value="ACD_ScHsp26_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.790:FF:000009">
        <property type="entry name" value="17.6 kDa class I heat shock protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031107">
        <property type="entry name" value="Small_HSP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11527">
        <property type="entry name" value="HEAT-SHOCK PROTEIN 20 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="6">
        <location>
            <begin position="37"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9684439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="151" mass="17170" checksum="5DBB799EA62C1637" modified="2024-01-24" version="1">MSLIPSFFSGRRNNMFDMWDPFQDFPFTGGALSVPGETASFANTRIDWKETPEAHVFKADLPGVKKEEVKVEVEEGRILQISGDRRVEKEEKNDKWHRVERSSGKFMRRFRLPENVKVEEMKAGMENGVLTVTVPKAEVKKPGVKAIDISG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z8Y9</accession>
    <name>A0AA38Z8Y9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Auxin response factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_016870</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Auxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs). Could act as transcriptional activator or repressor. Formation of heterodimers with Aux/IAA proteins may alter their ability to modulate early auxin response genes expression.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 10">Homodimers and heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ARF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z8Y9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007389">
        <property type="term" value="P:pattern specification process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048829">
        <property type="term" value="P:root cap development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd10017">
        <property type="entry name" value="B3_DNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.1040:FF:000002">
        <property type="entry name" value="Auxin response factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.330.10:FF:000001">
        <property type="entry name" value="Auxin response factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.330.10">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010525">
        <property type="entry name" value="ARF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044835">
        <property type="entry name" value="ARF_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003340">
        <property type="entry name" value="B3_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015300">
        <property type="entry name" value="DNA-bd_pseudobarrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31384:SF160">
        <property type="entry name" value="AUXIN RESPONSE FACTOR 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31384">
        <property type="entry name" value="AUXIN RESPONSE FACTOR 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06507">
        <property type="entry name" value="ARF_AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02362">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01019">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101936">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50863">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0927">Auxin signaling pathway</keyword>
    <keyword evidence="5 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TF-B3" evidence="12">
        <location>
            <begin position="117"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="PB1" evidence="13">
        <location>
            <begin position="600"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="544"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="544"/>
            <end position="578"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9684616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="683" mass="75377" checksum="3908B0926A167CAB" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z9G8</accession>
    <name>A0AA38Z9G8_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_016953</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z9G8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041304778" evidence="2">
        <location>
            <begin position="23"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9684737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="375" mass="29484" checksum="88F4F02907EEE013" modified="2024-01-24" version="1">MGSSKVMGFAFLVLLLLDLSFAARRLKEYGADGGGGGDGGGEGGGGGGGSAVGAGSGYGSGSGSGYGGADGNGYAGGGGGGGASGGGGGGGRAGAPGSGSGYGSGYGSSSGHGSGSGGGKGGGRGGGSGSGSGLRISDRDGNASGGGGGSGSKDVKGSASSGYGSGHAAGGGGDGHGSGSGSGYGSGSGGGVGVGGGGVGGGGGGGEGGGGGGGSAIGAGSGYGSGYGSGSGSGYGGTDGDGYASGGGGGGGGGGGGGGGGAGASGSGSGYGSGSGSGSGYGSGNGGGKGGGGGGGSGGGGGGGSGSGVGSGSGYGSGSGSGYGSGSGGGKGGGGGGGSGGGGGGGGGTGVGSGSGSGYGSGSGSGYGGGEGGSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZAQ5</accession>
    <name>A0AA38ZAQ5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Co-chaperone protein p23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_017212</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Acts as a co-chaperone for HSP90.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Interacts with HSP90 in an ATP-dependent manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the p23/wos2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZAQ5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051879">
        <property type="term" value="F:Hsp90 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051131">
        <property type="term" value="P:chaperone-mediated protein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007052">
        <property type="entry name" value="CS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045250">
        <property type="entry name" value="p23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22932:SF10">
        <property type="entry name" value="CO-CHAPERONE PROTEIN P23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22932">
        <property type="entry name" value="TELOMERASE-BINDING PROTEIN P23 HSP90 CO-CHAPERONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51203">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CS" evidence="4">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="86"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="93"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="116"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00025733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU369032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9685093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="132" mass="15241" checksum="6D02336F34C5A1BE" modified="2024-01-24" version="1">MFSATKDDVPYEVDIELFDKIKVEDSKCNVGVRSIVYVIAKAEKKWWARLIKNEGKPPVFLKVDWDKWVDEEDDNEKVGMDFDDMDFSKLDMGEDDFDMDESKDEEEGFEAEEEEGKGKEKQVAALAEEAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZBN4</accession>
    <name>A0AA38ZBN4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Germin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_015092</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May play a role in plant defense. Probably has no oxalate oxidase activity even if the active site is conserved.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Oligomer (believed to be a pentamer but probably hexamer).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Secreted</location>
            <location evidence="2 13">Extracellular space</location>
            <location evidence="2 13">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the germin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZBN4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02241">
        <property type="entry name" value="cupin_OxOx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000098">
        <property type="entry name" value="Germin-like protein 9-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006045">
        <property type="entry name" value="Cupin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001929">
        <property type="entry name" value="Germin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31238">
        <property type="entry name" value="GERMIN-LIKE PROTEIN SUBFAMILY 3 MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00190">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00325">
        <property type="entry name" value="GERMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00835">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0052">Apoplast</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 11 13" id="KW-0464">Manganese</keyword>
    <keyword evidence="7 11 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Cupin type-1" evidence="14">
        <location>
            <begin position="32"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU366015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9686040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="185" mass="19633" checksum="40E35CF720882033" modified="2024-01-24" version="1">MAGDPNILSDFILPANQSAVDGSFFTFTDMRVLIGAPEPTNFKVLKASLAEFPALNGQSVSTAVLQFPADAVNPPHTHPRSAELLFLIFGSLQVGFVDTTNKLYTQTLQEGDMFVFPKGLVHFQYNVDSKHSALAVSAFGSANAGTVSVPNSVFTTGIDDDILAKSFKTDVATIQAIKAGLAPKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZC79</accession>
    <name>A0AA38ZC79_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_015309</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZC79"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041366648" evidence="1">
        <location>
            <begin position="30"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9686346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="72" mass="8062" checksum="7DD1890569EB8995" modified="2024-01-24" version="1">MGYFYQCRNLMCFLLVLAMVLSLMPALQARPLSGVEVRRYRTEEQLQKLRELAAMLPKGVPIPPSGPSPGIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZDF5</accession>
    <name>A0AA38ZDF5_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_015397</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CNOT9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZDF5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030014">
        <property type="term" value="C:CCR4-NOT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000334">
        <property type="entry name" value="Cell differentiation protein-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007216">
        <property type="entry name" value="CNOT9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12262">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04078">
        <property type="entry name" value="Rcd1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9686473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="323" mass="36131" checksum="B7ACB291BA5611D5" modified="2024-01-24" version="1">MSNFSFSDWSHFLDQWGASSSSSSSNANHMPEKITPTTTSVEKLIKDIDNPLTREHALFLLSRNRGIRADLAPLLWNSSGTIYLLLQEITSAYRILSSPELVARATNNVCNALALLQCVASNPDTRMNLIQANIPCYLYPFLEAKNKEKPYEYLRLTSLGVIGALVKVDNAEIVHFLLQSEIVPLCLRCMEVGNELSKTVATFIIQKILVQDEGLRYFCSFAERFFALARVMGTMVDNLVEEPSQRLLKHIIHCYVRLSNCPRACDGFKICLPIRLRDAAILNLVQDDPVALEWLRQLFFNIATGNRVSSSAIGETLGSLLAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZDG8</accession>
    <name>A0AA38ZDG8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein YIP</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_015738</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Golgi apparatus membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the YIP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZDG8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006977">
        <property type="entry name" value="Yip1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039765">
        <property type="entry name" value="Yip5/YIPF1/YIPF2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12822:SF5">
        <property type="entry name" value="PROTEIN YIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12822">
        <property type="entry name" value="PROTEIN YIPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04893">
        <property type="entry name" value="Yip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="142"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="179"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="203"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="230"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Yip1" evidence="7">
        <location>
            <begin position="99"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9687001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="254" mass="29029" checksum="9187D4D7A98FB91F" modified="2024-01-24" version="1">MDEFLNSTISPTYLFITNIIQFNIDEQSIISFAILFNDPFVSCHFIHVLDGSSQCNKSWMCPYFHLNGLEPTIFPFFSIILERILKHFIIWSTVTSFPHIYGLIWTSTTLIFVIVALGNYATYLTKKWSHSSATWSFDVSYLNLPFCSIYGYALLVAVVFYFLLQYLGSNASLVRCWCLWGYSVFILILSSVLLVIPIKFLRWLIIVLVGGASACFVALNLIRYIQGNDISLVIVASFVFQMGLALFIKICFFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZDY1</accession>
    <name>A0AA38ZDY1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_015923</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZDY1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9687246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="87" mass="8464" checksum="7C5D94F954A585A7" modified="2024-01-24" version="1">MAALTSARVSADPGIGAGAVSTTTWNARDMRDDATGSPGLDRGPMIGMKTNGVRLASIFGGVGVGGGLAGLWTPKASSAAVAGNGFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZE79</accession>
    <name>A0AA38ZE79_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CC-NBS-LRR protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_016040</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZE79"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766:SF70">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RGA4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766">
        <property type="entry name" value="PLANT BROAD-SPECTRUM MILDEW RESISTANCE PROTEIN RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0611">Plant defense</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9687390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="124" mass="13517" checksum="74823B9436822A4B" modified="2024-01-24" version="1">MFSLGNSNSKSPSHLHDCHPPLSFTLLMCLPSNLNSLTITNCNKFTSQVEWGLQGLASLPSLKISGLPNLRSLDSLGLQLLTSLQKLEIHDCPKLQSLTEELLPTSLSVLTIQSCPLLKGQCKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZJD9</accession>
    <name>A0AA38ZJD9_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_012694</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZJD9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010333">
        <property type="term" value="F:terpene synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016102">
        <property type="term" value="P:diterpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00684">
        <property type="entry name" value="Terpene_cyclase_plant_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.600.10:FF:000007">
        <property type="entry name" value="Isoprene synthase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.130:FF:000001">
        <property type="entry name" value="Isoprene synthase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.130">
        <property type="entry name" value="Terpene synthase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034741">
        <property type="entry name" value="Terpene_cyclase-like_1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044814">
        <property type="entry name" value="Terpene_cyclase_plant_C1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001906">
        <property type="entry name" value="Terpene_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036965">
        <property type="entry name" value="Terpene_synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050148">
        <property type="entry name" value="Terpene_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005630">
        <property type="entry name" value="Terpene_synthase_metal-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225">
        <property type="entry name" value="OS04G0344100 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225:SF0">
        <property type="entry name" value="S-(+)-LINALOOL SYNTHASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01397">
        <property type="entry name" value="Terpene_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03936">
        <property type="entry name" value="Terpene_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01019">
        <property type="entry name" value="Terpene_Cyclase_Like_1_C_Termi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Terpene synthase N-terminal" evidence="5">
        <location>
            <begin position="62"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="Terpene synthase metal-binding" evidence="6">
        <location>
            <begin position="282"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9690168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="576" mass="66543" checksum="3EF6FFD7BED4D8B5" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZKV5</accession>
    <name>A0AA38ZKV5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S ribosomal protein L18a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_013227</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZKV5"/>
    <dbReference type="SMR" id="A0AA38ZKV5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.10:FF:000001">
        <property type="entry name" value="60S ribosomal protein L18a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L18a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00273">
        <property type="entry name" value="Ribosomal_eL20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028877">
        <property type="entry name" value="Ribosomal_eL20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023573">
        <property type="entry name" value="Ribosomal_eL20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021138">
        <property type="entry name" value="Ribosomal_eL20_eukaryotes"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10052">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L18A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01775">
        <property type="entry name" value="Ribosomal_L18A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002190">
        <property type="entry name" value="Ribosomal_L18a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160374">
        <property type="entry name" value="RplX-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein eL20" evidence="5">
        <location>
            <begin position="7"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR002190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9690963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="178" mass="21258" checksum="D7B76B3A372A5E66" modified="2024-01-24" version="1">MVTFRFHQYQVVGRALPSESDEHPKIYRMKLWATNEVRAKSKFWYFLRKLKKVKKSNGQVLAINEIFEKNPTTIKNYGIWLRYQSRTGYHNMYKEYRDTTLNGAVEQMYAEMASRHRVRSPCIQIIKTATIPAKLCKRESSKQFHNSKIKFPLTFRKVRPPTRKLKTTYKASRPNLFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZLL0</accession>
    <name>A0AA38ZLL0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_013497</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZLL0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45631">
        <property type="entry name" value="OS07G0107800 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45631:SF68">
        <property type="entry name" value="REPEAT FAMILY PROTEIN, PUTATIVE, EXPRESSED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041293113" description="Protein kinase domain-containing protein" evidence="3">
        <location>
            <begin position="26"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="222"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="274"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="518"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="523"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9691338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="542" mass="61544" checksum="94B6C1710984D3E5" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZLL4</accession>
    <name>A0AA38ZLL4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein FLX-like 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_013140</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FLX family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZLL4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009908">
        <property type="term" value="P:flower development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040353">
        <property type="entry name" value="FLX/FLX-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33405:SF7">
        <property type="entry name" value="PROTEIN FLX-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33405">
        <property type="entry name" value="PROTEIN FLX-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0221">Differentiation</keyword>
    <keyword evidence="5" id="KW-0287">Flowering</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="277"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="141"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="214"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9690837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="307" mass="33458" checksum="975299118352BBEF" modified="2024-01-24" version="1">MSGRNRGPPIPMKGGLHEPPFRGRGLGPMPHPALLDEPQFGRGAGGPRPGTLIPHPAAIIEDRLALQHQDIQALLIDNQRLAATHVALKQEVEAAQHEIKRMAHVAGSAQTEMDLQLREAFEKVMKLEADLRASDAMRAEVMQVRADIQQLTAARQELTSQAEGLSQDLNRANLDLQQVPLLKGEIEGMRQELQRARAAIEYEKKGYAENYEHGQAMEKNLIAMAREMEKLRAELANAEKRARAAAAVGNQIYGGSYGNPETGYGGNPYPAGYGMNPGQGSAEGVPQYGPGPGSWGTYDMQRAHGHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZM72</accession>
    <name>A0AA38ZM72_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GAG-pre-integrase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_013539</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZM72"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029472">
        <property type="entry name" value="Copia-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025724">
        <property type="entry name" value="GAG-pre-integrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054722">
        <property type="entry name" value="PolX-like_BBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34222:SF40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34222">
        <property type="entry name" value="GAG_PRE-INTEGRS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13976">
        <property type="entry name" value="gag_pre-integrs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22936">
        <property type="entry name" value="Pol_BBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14244">
        <property type="entry name" value="Retrotran_gag_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Retrotransposon Copia-like N-terminal" evidence="3">
        <location>
            <begin position="32"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="Retrovirus-related Pol polyprotein from transposon TNT 1-94-like beta-barrel" evidence="4">
        <location>
            <begin position="354"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="domain" description="GAG-pre-integrase" evidence="2">
        <location>
            <begin position="460"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF22936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9691385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="588" mass="66804" checksum="5E4446396053DE00" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZN06</accession>
    <name>A0AA38ZN06_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Nucleosome assembly protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_011264</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the nucleosome assembly protein (NAP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZN06"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1500:FF:000001">
        <property type="entry name" value="Nucleosome assembly protein 1-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1120.90:FF:000005">
        <property type="entry name" value="Nucleosome assembly protein11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.90">
        <property type="entry name" value="Nucleosome assembly protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037231">
        <property type="entry name" value="NAP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002164">
        <property type="entry name" value="NAP_family"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11875">
        <property type="entry name" value="TESTIS-SPECIFIC Y-ENCODED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00956">
        <property type="entry name" value="NAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143113">
        <property type="entry name" value="NAP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="301"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="307"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9692091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="371" mass="42612" checksum="F10C3EC280A7B77B" modified="2024-01-24" version="1">MSNDKDQFNIADLGAALPAAAAALSAEDRAGLVNALKNKLQNLAGQHSDVLETLSPNVRKRVEFLREIQGQHDELEAKFFEERAALEAKYQKLYEPLYTKRYEIVNGVVEVEGVTNEAPMDQEEDKATEEKGVPNFWLTAMKTNEVLAEEISERDEGALKYLKDIKWYRIDNPKGFKLEFYFDANPYFKNSILTKTYHMIDDDEPILEKAIGTEIEWHPGKSLTQKLLKKKPRKGSKNAKPITKTENCESFFNFFNPPQVPEDEEDIDEDVAEELQNQMEQDYDIGSTIRDKIIPHAVSWFTGEAVQGDEFEDIEDDDDEDEEDEDEEEDDEEDDEDEDEEEEDKSRKKKSGRSHAGEGQQGERPPECKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZPV8</accession>
    <name>A0AA38ZPV8_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_011583</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZPV8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9692592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="65" mass="7116" checksum="C91C78929048EB2B" modified="2024-01-24" version="1">MASVAPLPLSLADRDVQVIVEAYKDEPGNPKYAFKHVLFSAQGRPRFGVSLSLFVNCLILSQSHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZQK2</accession>
    <name>A0AA38ZQK2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_012284</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9693441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZQK2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020847">
        <property type="entry name" value="AP_endonuclease_F1_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF78">
        <property type="entry name" value="OS12G0587133 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00726">
        <property type="entry name" value="AP_NUCLEASE_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="470"/>
            <end position="723"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9693441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="912" mass="105448" checksum="AC8F941100A948C3" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZQN3</accession>
    <name>A0AA38ZQN3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Signal peptidase complex catalytic subunit SEC11</fullName>
            <ecNumber evidence="4">3.4.21.89</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Signal peptidase complex catalytic subunit sec11</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_011625</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalytic component of the signal peptidase complex (SPC) which catalyzes the cleavage of N-terminal signal sequences from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum. Specifically cleaves N-terminal signal peptides that contain a hydrophobic alpha-helix (h-region) shorter than 18-20 amino acids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 17">
            <text>Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.</text>
            <dbReference type="EC" id="3.4.21.89"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase S26B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.89" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZQN3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005787">
        <property type="term" value="C:signal peptidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009003">
        <property type="term" value="F:signal peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06530">
        <property type="entry name" value="S26_SPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.109.10:FF:000003">
        <property type="entry name" value="Signal peptidase complex catalytic subunit SEC11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.109.10">
        <property type="entry name" value="Umud Fragment, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015927">
        <property type="entry name" value="Peptidase_S24_S26A/B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019533">
        <property type="entry name" value="Peptidase_S26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001733">
        <property type="entry name" value="Peptidase_S26B"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02228">
        <property type="entry name" value="sigpep_I_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10806">
        <property type="entry name" value="SIGNAL PEPTIDASE COMPLEX CATALYTIC SUBUNIT SEC11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10806:SF6">
        <property type="entry name" value="SIGNAL PEPTIDASE COMPLEX CATALYTIC SUBUNIT SEC11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00717">
        <property type="entry name" value="Peptidase_S24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00728">
        <property type="entry name" value="SIGNALPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="LexA/Signal peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Peptidase S24/S26A/S26B/S26C" evidence="14">
        <location>
            <begin position="36"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00021755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9692639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="180" mass="19989" checksum="BD93C0CC20A380E7" modified="2024-01-24" version="1">MGWIGETVDSIKSIQIRQVLSQAVSLGMIVTSALIIWKALMCITGSESPVVVVLSGSMEPGFKRGDILFLHMSKDPIRTGEIVVFNVDGREIPIVHRVIKVHERQDTGEVDVLTKGDNNYGDDRLLYAHGQLWLQRHHIMGRAVGFLPYVGWVTIIMTEKPLIKYVLIGALGLLVITSKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZT52</accession>
    <name>A0AA38ZT52_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_010158</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Involved in membrane trafficking from the Golgi to the plasma membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Interacts with PI5K2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZT52"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01867">
        <property type="entry name" value="Rab8_Rab10_Rab13_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000308">
        <property type="entry name" value="ras-related protein RABE1c-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050305">
        <property type="entry name" value="Small_GTPase_Rab"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47980">
        <property type="entry name" value="LD44762P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0636">Prenylation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00054463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00063571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9694534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="216" mass="23909" checksum="91F5F61C485A98FA" modified="2024-01-24" version="1">MAAPPARARADYDYLIKLLLIGDSGVGKSCLLLRFSDGSFTTSFITTIGIDFKIRTIELDGKRIKLQIWDTAGQERFRTITTAYYRGAMGILLVYDVTDESSFNNIRNWIRNIEQHASDNVNKILVGNKADMDESKRAVPTSKGQALADEYGIKFFETSAKTNLNVEEVFFSIGRDIKQRLAETDSKAEPHTIRINPLDQAAGSAETPAKSACCGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZUW4</accession>
    <name>A0AA38ZUW4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DNA-directed RNA polymerase subunit</fullName>
            <ecNumber evidence="12">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_010966</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the RNA polymerase beta' chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="12"/>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02735">
        <property type="entry name" value="RNAP_I_Rpa1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01435">
        <property type="entry name" value="RNAP_I_RPA1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.20:FF:000019">
        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.30:FF:000006">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.390:FF:000005">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.274.100:FF:000015">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.180:FF:000007">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.860.120:FF:000006">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2850">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.180">
        <property type="entry name" value="RNA polymerase ii"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.120">
        <property type="entry name" value="RNA polymerase II, clamp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.274.100">
        <property type="entry name" value="RNA polymerase Rpb1, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047107">
        <property type="entry name" value="DNA-dir_RNA_pol1_lsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015699">
        <property type="entry name" value="DNA-dir_RNA_pol1_lsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045867">
        <property type="entry name" value="DNA-dir_RpoC_beta_prime"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000722">
        <property type="entry name" value="RNA_pol_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006592">
        <property type="entry name" value="RNA_pol_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007080">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007066">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042102">
        <property type="entry name" value="RNA_pol_Rpb1_3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007083">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007081">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044893">
        <property type="entry name" value="RNA_pol_Rpb1_clamp_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038120">
        <property type="entry name" value="Rpb1_funnel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376:SF11">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04997">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00623">
        <property type="entry name" value="RNA_pol_Rpb1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04983">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05000">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04998">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00663">
        <property type="entry name" value="RPOLA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 12" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 12" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="RNA polymerase N-terminal" evidence="14">
        <location>
            <begin position="356"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="769"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1335"/>
            <end position="1403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1418"/>
            <end position="1458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="1357"/>
            <end position="1372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="1384"/>
            <end position="1399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="1440"/>
            <end position="1458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9695751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1691" mass="188635" checksum="6C69F50EED6DECB5" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZY19</accession>
    <name>A0AA38ZY19_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cyclin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_009305</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclin family. Cyclin C subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZY19"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000058">
        <property type="entry name" value="cyclin-C1-2-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043198">
        <property type="entry name" value="Cyclin/Ssn8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10026">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028758">
        <property type="entry name" value="Cyclin, C/H/G types"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3 5" id="KW-0195">Cyclin</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="6">
        <location>
            <begin position="46"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="6">
        <location>
            <begin position="149"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9697425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="251" mass="29478" checksum="78D8879B7C544398" modified="2024-01-24" version="1">MAANFWTSSHHKQLLDQEEVDVVHPLDKEKGITLEDFKLIKMHMANYIGRLGQNVKVRQRVVATAITYMRRVYTRKAMTEYDPRLVAPTSLYLAAKAEESTVQARLLVFYIRKLYSDEKYRYEIKDILEMEMKILEALNYYLVVFHPYRALSQLLQDANLNDLTQLSWGIVNDTYKMDLILIYPPHLIALACIYIASVHKDKETTAWFEELRVDMNVVKNISMEILDFYDGHRTITEDRITTTFNKLALRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZYY2</accession>
    <name>A0AA38ZYY2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dirigent protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_007094</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Secreted</location>
            <location evidence="4">Extracellular space</location>
            <location evidence="4">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the plant dirigent protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9697801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZYY2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009699">
        <property type="term" value="P:phenylpropanoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.480.10">
        <property type="entry name" value="Allene oxide cyclase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044859">
        <property type="entry name" value="Allene_oxi_cyc_Dirigent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004265">
        <property type="entry name" value="Dirigent"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21495">
        <property type="entry name" value="NUCLEOPORIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03018">
        <property type="entry name" value="Dirigent"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0052">Apoplast</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9697801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="192" mass="21231" checksum="E68F469B320C93AD" modified="2024-01-24" version="1">MAKALLISPSYFIFSIFLVFSYVTIPVFGEEYSYVKTIDFEKMNLPKEKVTHFQLYWQDVVSGSNATSVTVIEALNNSTFFGSVNIIDNSLTVGPNLSSKSVGKSQGFYASTGLEESSLLMVMNFAFTDGKYNGSTFTVVGRNNVNAKVREMPIIGGSGLFRFARGYVLASTYSFSDNGDGTIEYNCYVIHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZYY5</accession>
    <name>A0AA38ZYY5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_007067</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(E)-4-coumaroyl-CoA + 2-oxoglutarate + O2 = (E)-2,4-dihydroxycinnamoyl-CoA + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:57868"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:85008"/>
            <dbReference type="ChEBI" id="CHEBI:142398"/>
            <dbReference type="EC" id="1.14.11.62"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(E)-feruloyl-CoA + 2-oxoglutarate + O2 = (E)-6-hydroxyferuloyl-CoA + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:57856"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:87305"/>
            <dbReference type="ChEBI" id="CHEBI:142390"/>
            <dbReference type="EC" id="1.14.11.61"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Phenylpropanoid metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9697772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZYY5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102312">
        <property type="term" value="F:4-coumaroyl 2'-hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009805">
        <property type="term" value="P:coumarin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.330:FF:000023">
        <property type="entry name" value="Feruloyl CoA ortho-hydroxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10209:SF243">
        <property type="entry name" value="FERULOYL COA ORTHO-HYDROXYLASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10209">
        <property type="entry name" value="OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="7 10" id="KW-0408">Iron</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="11">
        <location>
            <begin position="202"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9697772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="358" mass="40256" checksum="57CB38B6F94F5FF6" modified="2024-01-24" version="1">MAPTMVVSATNSWDITDFVVNQGHGVKGLSEMKLPSLPKQFIQPPEERIDASKVVREECIPVIDMAGLDDPKVAELICDAAEKWGFFQIVNHGVPIGVLEDVKEATHRFFALPGEEKKKYSKENSNSNSVRFGTSFTPQAEKSLEWKDYLSLFYVSEDEASALWPSVCKDQALEYMKRSELVIRQLLEVLMRRLNVKEIDEKKESLLMGSMRINLNYYPICPNPELTVGVGRHSDVSTLTVLLQDDIGGLYVRGVHDTWVHVPPIRGSLVINIGDALQIMSNGRYKSIEHRVIANGSSNRVSVPIFVNPRPSEVIGPLPEVLEGGEEPLYKQVLYSDYVKHFFRKAHDGKNTVEFAKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A1Q9</accession>
    <name>A0AA39A1Q9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Carboxypeptidase</fullName>
            <ecNumber evidence="9">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_008108</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the peptidase S10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="9"/>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9699345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A1Q9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000123">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF3">
        <property type="entry name" value="RETINOID-INDUCIBLE SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 9" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041488620" description="Carboxypeptidase" evidence="9">
        <location>
            <begin position="29"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9699345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="465" mass="51736" checksum="237A55D7A993D368" modified="2024-01-24" version="1">MFLALEKMEKWCFLLVLLVLMFGPWVNGGMAMAYRSQDGTEEWGYVEVRPKAHMFWWLYRSPYRVESPSKPWPIILWLQGGPGASGVGIGNFQEIGPLGTDLKPRNSTWLRKADLLFVDNPVGTGYSFVEDTKLLVKTDVEAAVDLTTLLKEIFNRNESLQQSPLYIVAESYGGKFAVTLGLAALEAIEAGNLKLKLGGVALGDSWISPEDFVFSWGPLLKDVSRIDNQGLQKSNSLAGKIRQQLIDGQYVDATSSWSELEGVISRSSNSVDFYNFLLDSNMDPISLTSVELREQIAKRRYLRYLDSLRLSPGGDVDIDTLMNGIIKDKLKIIPKNVSWGGQSDLVFTTLSGDFMKPRIKEVDELLAKGVNVTIYNGQLDLICATMGTEAWVEKLNWDGLKEFLSMKRTPLYCGGEGGTKGFTKSYKNLHFYWILKAGHFVPVDQPCIALNMVGGITHSPVASIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A4A7</accession>
    <name>A0AA39A4A7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_006166</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A4A7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000537">
        <property type="entry name" value="Putative receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000178">
        <property type="entry name" value="serine/threonine-protein kinase PBS1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045874">
        <property type="entry name" value="LRK10/LRL21-25-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27009">
        <property type="entry name" value="RUST RESISTANCE KINASE LR10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="105"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="400"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="424"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9700710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="434" mass="49327" checksum="EB82E5A23F0AC955" modified="2024-01-24" version="1">MPSASEVFGTITVQSKFGIVIVMPLLILLLRRIMRRMPQNLDIGAEINALITGRLPQREMGPQEARRTESTGLVDRSGTVEIFIQDMLNEKPVRFSPQQLAAFTQNFSTKLGSGGFGHVYRGVLPDGVQIAVKVLKHNSGQDKRMEEQFMAEVRTIGRTYHRNLVRLYGFCFDSQLKALVYEYMESGSLDTVLFGREHRIEWEKLYEIAVGAAKGLKYLHDDCHKRIIHHDIKPGNVLLDSNFCPKLADFGLAKLSNMDSTHENFSGGGTPGYAAPEVWMPFQVTYKCDVYSFGMMLFEIVGRRRNFYNFSGEDQDWFPRQVWDKFDEGELEGLLLERGIKEKEMVKAKKMCMVALWCVQYLPQDRPSMDQVLKILEGGDQIATPKNPFQHLALSDMPPISIESSTNSRSDETTDNSSAWFMNKYEREEASSSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A4V6</accession>
    <name>A0AA39A4V6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Pre-mRNA-processing factor 19</fullName>
            <ecNumber evidence="19">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PVL29_006214</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Probable ubiquitin-protein ligase which is mainly involved pre-mRNA splicing and DNA repair. Component of the MAC complex that probably regulates defense responses through transcriptional control and thereby is essential for plant innate immunity.</text>
    </comment>
    <comment type="function">
        <text evidence="19">Ubiquitin-protein ligase which is mainly involved pre-mRNA splicing and DNA repair. Required for pre-mRNA splicing as component of the spliceosome.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 19">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Homotetramer. Component of the multiprotein assembly MOS4-associated complex (MAC) at least composed of MOS4, CDC5, PRL1 and PRP19 which is related to the PRP19C/Prp19 complex/NTC/Nineteen complex identified in other organisms. Associated with the spliceosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 19">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 19">Belongs to the WD repeat PRP19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="19"/>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A4V6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071006">
        <property type="term" value="C:U2-type catalytic step 1 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070534">
        <property type="term" value="P:protein K63-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16656">
        <property type="entry name" value="RING-Ubox_PRP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000043">
        <property type="entry name" value="pre-mRNA-processing factor 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000027">
        <property type="entry name" value="Pre-mRNA-processing factor 19, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038959">
        <property type="entry name" value="Prp19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013915">
        <property type="entry name" value="Prp19_cc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055340">
        <property type="entry name" value="RING-Ubox_PRP19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43995">
        <property type="entry name" value="PRE-MRNA-PROCESSING FACTOR 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43995:SF1">
        <property type="entry name" value="PRE-MRNA-PROCESSING FACTOR 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08606">
        <property type="entry name" value="Prp19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24814">
        <property type="entry name" value="WD40_Prp19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51698">
        <property type="entry name" value="U_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 19" id="KW-0227">DNA damage</keyword>
    <keyword evidence="14 19" id="KW-0234">DNA repair</keyword>
    <keyword evidence="12" id="KW-0391">Immunity</keyword>
    <keyword evidence="5" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="6 19" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="13 19" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="15 19" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 19" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="7 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="11 19" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="4 18" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="U-box" evidence="20">
        <location>
            <begin position="54"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="18">
        <location>
            <begin position="320"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="18">
        <location>
            <begin position="365"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="18">
        <location>
            <begin position="417"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="18">
        <location>
            <begin position="451"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00056434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00065458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU367101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAJ9700789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="582" mass="63641" checksum="601CA264B5F60E63" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A557</accession>
    <name>A0AA39A557_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_006396</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A557"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="147"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9701026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="193" mass="21812" checksum="731BD02A65BAC840" modified="2024-01-24" version="1">MITRSITTANPSSIIEQKKKKCKIPHNIQKLFFFSNMGASTAMPASGLGGTPRRTMTSIEFLKKNGKGIKQDKLMGCRDTDKSTDISEENQTKRPKLRENDLDCDGAELNGGLEMEVVSALAPASHHKGLARKVLLDVVGLLNLRLWSLWTLSQIFFNFQIFFFLLFLLILNKHEYDFHNSKTIEFVGLIHAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A5U5</accession>
    <name>A0AA39A5U5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_006758</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A5U5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053213">
        <property type="entry name" value="RLP29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48009">
        <property type="entry name" value="LEUCINE-RICH REPEAT (LRR) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48009:SF7">
        <property type="entry name" value="LEUCINE-RICH REPEAT (LRR) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="4">
        <location>
            <begin position="35"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9701535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="131" mass="14159" checksum="B2AE3AB51A7FA2E0" modified="2024-01-24" version="1">MANQRVSLSCSGLSSIVVAVLITFISRPAHSRTLESDTEVLRSFKASFDPNSVPPYLFISTWDFRMDPCESSGELFLGILCSTPVDNSSSRVTAIDLDGIGSDGFLPPVIGNLTEITALSFKQKQLSRAIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A5X4</accession>
    <name>A0AA39A5X4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_006803</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A5X4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000242">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF415">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="44"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="75"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="110"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9701580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="195" mass="21958" checksum="A562BC807D4E8980" modified="2024-01-24" version="1">MQMVGIEPDEISLVSVLPACAQLGALELRKWIHFYAVKAGFLRNICVCNALIEMYAKRGSIEEGRRLFDQMNERDVISWSTMIVGLANHGRAREAIELFQEMQNAKIEPNIITFVSLLSACAHAGLLNEGLRYFESMKRDYNIEPGVEHYGCLVNLLGLSGRLDQALELIKTMPMKLTSPKIAVKPDTHQANFYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A730</accession>
    <name>A0AA39A730_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_006889</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A730"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050486">
        <property type="entry name" value="Mannose-1P_guanyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22572">
        <property type="entry name" value="SUGAR-1-PHOSPHATE GUANYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041448579" evidence="1">
        <location>
            <begin position="23"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9701705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="341" mass="38712" checksum="62EBFEF496BF392A" modified="2024-01-24" version="1">MEYPRSILSSLSLVSILYQASGNCITNSYMKPVIRNDSKPMVHHPISACKRIPNLAQIFLVGFYEERELALYVSSVSNELKVPIRYLKEDKPHGPAGGLYYFRTVIKYDGMGTVLVIKTFVSDLINCGVYVFTPDIFIAIQGFSTHREDRASLRRVSSFEAFQSAARTHPTDFVRLDQDILSPLAGKKQLYTDETTDFWEQIKTPGMSLKCSSLYLDQFRFTIPVFISLIPLPLTHPTLHPYTLNNAFSYLSPLQNLTSSSSLLPTPEHYTLAGWKTCSIWKGVIWNGCGLQDDMIEFIKLIHAKINWTLLGAQHIWCEGGLKNKINTKSMEEKKIDLENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A7L8</accession>
    <name>A0AA39A7L8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ENTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_006610</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Clathrin-coated vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the epsin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A7L8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030125">
        <property type="term" value="C:clathrin vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03571">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000006">
        <property type="entry name" value="Clathrin interactor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276:SF91">
        <property type="entry name" value="CLATHRIN INTERACTOR EPSIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276">
        <property type="entry name" value="EPSIN/ENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01417">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="7">
        <location>
            <begin position="18"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="150"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="405"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="545"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="815"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="957"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="150"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="183"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="227"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="266"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="324"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="334"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="468"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="519"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="567"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="607"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="633"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="717"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="747"/>
            <end position="759"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9701339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="991" mass="103844" checksum="A89C1D4B0DD5FD5F" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A9N9</accession>
    <name>A0AA39A9N9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">15-cis-phytoene synthase</fullName>
            <ecNumber evidence="4">2.5.1.32</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_004676</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 (2E,6E,10E)-geranylgeranyl diphosphate = 15-cis-phytoene + 2 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:34475"/>
            <dbReference type="ChEBI" id="CHEBI:27787"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58756"/>
            <dbReference type="EC" id="2.5.1.32"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carotenoid biosynthesis; phytoene biosynthesis; all-trans-phytoene from geranylgeranyl diphosphate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the phytoene/squalene synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.32" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A9N9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009536">
        <property type="term" value="C:plastid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046905">
        <property type="term" value="F:15-cis-phytoene synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004311">
        <property type="term" value="F:geranylgeranyl diphosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051996">
        <property type="term" value="F:squalene synthase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016117">
        <property type="term" value="P:carotenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00683">
        <property type="entry name" value="Trans_IPPS_HH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.600.10:FF:000004">
        <property type="entry name" value="Phytoene synthase chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002060">
        <property type="entry name" value="Squ/phyt_synthse"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019845">
        <property type="entry name" value="Squalene/phytoene_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044843">
        <property type="entry name" value="Trans_IPPS_bact-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033904">
        <property type="entry name" value="Trans_IPPS_HH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31480">
        <property type="entry name" value="BIFUNCTIONAL LYCOPENE CYCLASE/PHYTOENE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00494">
        <property type="entry name" value="SQS_PSY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01212">
        <property type="entry name" value="Phytoene_synthase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01018">
        <property type="entry name" value="Squalene/Phytoene_Synthase_Lik"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01044">
        <property type="entry name" value="SQUALEN_PHYTOEN_SYN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01045">
        <property type="entry name" value="SQUALEN_PHYTOEN_SYN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0125">Carotenoid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9703017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="437" mass="49613" checksum="38D74A0BEC3D832E" modified="2024-01-24" version="1">MSVALLWIVPPNKEASNSFGFLESIRDGNRALDSPWFIPRDQNLIFNGRPKKGRRQRCNSGSSGADLRYSYLGGSGLESRTNFPIFSSMVVNPTGEMAVSSEQKVYDVVLKQAALVKKQLRSSEELDVRPDIILPGTRSLLSEAYDRCGEVCAEYAKTFYLGTMLMTPERRRAIWAIYVWCRRTDELVDGPNASHITPTALDRWESRLEDLFQGRPFDMLDAALSDTVAKFPVDIQPFKDMIEGMRMDLRKSRYRNFDELYLYCYYVAGTVGLMSVPVMGIAPESQATTESVYNAALALGIANQLTNILRDVGEDARRGRIYLPQDELAQSGLSDEDIFMGRVTDKWRSFMKDQIKRARMFFDEAEKGITELSSASRWPVWASFLLYRQILDEIEANDYDNFTRRAYVSKAKKIVALPLAYTRSLVRPSRTTSLAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AAD4</accession>
    <name>A0AA39AAD4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_004979</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AAD4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015144">
        <property type="term" value="F:carbohydrate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000025">
        <property type="entry name" value="probable polyol transporter 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045262">
        <property type="entry name" value="STP/PLT_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23500:SF453">
        <property type="entry name" value="POLYOL TRANSPORTER 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23500">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 2, FACILITATED GLUCOSE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="6" id="KW-0769">Symport</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="18"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="112"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="145"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="175"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="315"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="342"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="375"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="441"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="11">
        <location>
            <begin position="22"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9703430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="503" mass="54116" checksum="2A84BB947A8273F1" modified="2024-01-24" version="1">MDSKEEGAVQSPTGLNKYACACAAVASMISIIFGYDTGVMSGAMLFIKEDLKVNELQVSVLAGILNVCALVGSLAAGRTSDFLGRRYTIVLASILFLVGSVLMGYAPNYAVLLTGRCTAGIGVGYALMIAPVYSAELSSPKSRGFLTSLPELGISTGILSGYLANYFMAALPLKLGWRLMLGIAAVPSLGLAFGILKMPESPRWLVMQGRLGDAEKILLQVSNTKEEAETRFRDIKIAAGIDEDCHEDVVKPPSNTHGGGVWKELLLRPTPAVRWMLLATIGIHFFEHATGIEAVMLFSPRILKKAGVHSKDKQLLATVGVGLTKLTFMSLSTLLIDRVGRRPLLLTSTTGMIVALTGLGIGLTMAERAKQKLYWALNLSFVATYTFVAFFNIGVAPVTWVYPAEIFPLKLRAQGASIGVAVNRGTNAAISISFIHIYEAITIGGAFFMFAGISVVAWIFFYFLLPETKGKPLEEMELLFTRGGRSKSKNKGIEVQPKGRIVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AAD8</accession>
    <name>A0AA39AAD8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_005262</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AAD8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000280">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000393">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At1g20230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001138">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At1g33350"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000442">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At3g49710"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF346">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="95"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="229"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="260"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="333"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="364"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="502"/>
            <end position="536"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9703911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="596" mass="66793" checksum="153E5D2A4ACB4AA3" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AD43</accession>
    <name>A0AA39AD43_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serine carboxypeptidase-like 18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_003381</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AD43"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019748">
        <property type="term" value="P:secondary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12670:FF:000002">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000072">
        <property type="entry name" value="Serine carboxypeptidase-like 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12670">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF329">
        <property type="entry name" value="SERINE CARBOXYPEPTIDASE-LIKE 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9705316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="513" mass="58835" checksum="2FCD56C39C94EF2C" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AF76</accession>
    <name>A0AA39AF76_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BED-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_003850</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AF76"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20908">
        <property type="entry name" value="SUF4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003656">
        <property type="entry name" value="Znf_BED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23215:SF0">
        <property type="entry name" value="BUB3-INTERACTING AND GLEBS MOTIF-CONTAINING PROTEIN ZNF207"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23215">
        <property type="entry name" value="ZINC FINGER PROTEIN 207"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50808">
        <property type="entry name" value="ZF_BED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BED-type" evidence="8">
        <location>
            <begin position="6"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="189"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="285"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9705935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="367" mass="39236" checksum="65ACA4B824DBCBDF" modified="2024-01-24" version="1">MGKKKKRAAKVFCYYCEREFEDEKILVQHQKAKHFKCHVCNKKLSTASGMAIHVLQVHKEAVSKVPNANPGRESTEIEIFGMQGIPDDILAAHYGEQDEDNPSKLAKVEVPPTNVVGVMPPGSVGMGFPPQSTFGAIPPIYNSAVAVPPGWPVPRPQPWFPQHPAVPVPPAAPLGLAQRPLFPIQNVTPPLPSATLQPSFQTTPPGPLSSSPTPVSQPLFPVVGTASIPSQNSQVTAAALSAPITSSTPSELNNPSYSSANTSMVNGYHAPSIQAGTLVSSHTYASGPNTSGPSIGPPPMIANKAPATQPASNEVYLVWDDEAMSMEERRMSLLKYQVHDETSQMNSVDAAIDRRISESRLAGRMAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AFW7</accession>
    <name>A0AA39AFW7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_002033</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant LTP family. PEARLI1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AFW7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01958">
        <property type="entry name" value="HPS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.110.10:FF:000003">
        <property type="entry name" value="pEARLI1-like lipid transfer protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.110.10">
        <property type="entry name" value="Plant lipid-transfer and hydrophobic proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036312">
        <property type="entry name" value="Bifun_inhib/LTP/seed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016140">
        <property type="entry name" value="Bifunc_inhib/LTP/seed_store"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027923">
        <property type="entry name" value="Hydrophob_seed_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051636">
        <property type="entry name" value="Plant_LTP/defense-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31731">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14547">
        <property type="entry name" value="Hydrophob_seed"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00499">
        <property type="entry name" value="AAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47699">
        <property type="entry name" value="Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041201153" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein" evidence="4">
        <location>
            <begin position="26"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical" evidence="5">
        <location>
            <begin position="52"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9706875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="135" mass="13836" checksum="1B36FCEC7142D6D0" modified="2024-01-24" version="1">MASKSSASVAFFLLVNLLFFAIVSACGTCPNPPKSRTNPRPTPSPSPARATCPKDTLKLGVCADLLGGLISVVVGTPPKTPCCSLIQGLADLEAAICLCTAIKANVLGINLNIPLSLSLLLNVCSKKVPPGFQCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AGV8</accession>
    <name>A0AA39AGV8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Endoglucanase</fullName>
            <ecNumber evidence="9">3.2.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_001548</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>Endohydrolysis of (1-&gt;4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 8 9">Belongs to the glycosyl hydrolase 9 (cellulase E) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.4" evidence="9"/>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AGV8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008810">
        <property type="term" value="F:cellulase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000020">
        <property type="entry name" value="Endoglucanase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001701">
        <property type="entry name" value="Glyco_hydro_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033126">
        <property type="entry name" value="Glyco_hydro_9_Asp/Glu_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22298">
        <property type="entry name" value="ENDO-1,4-BETA-GLUCANASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00759">
        <property type="entry name" value="Glyco_hydro_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00698">
        <property type="entry name" value="GH9_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8 9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4 9" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="6 8 9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 8 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 8 9" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041483053" description="Endoglucanase" evidence="9">
        <location>
            <begin position="24"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 9" evidence="10">
        <location>
            <begin position="36"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="462"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9706034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="509" mass="55802" checksum="4F75F4F577881A54" modified="2024-01-24" version="1">MGYGGMVWGLVLWCVLASRNGVAMEDERLLGLGHDYGEALGKAILFFEGQRSGKLPSSQRVKWRGDSALSDGMPENVKLIGGYYDAGDNVKFGWPMAFSVSLLSWAAVEYRREISSVKQLTYLLQAIRWGANFILRAHTSSTTLYTQVGDGNNDHQCWERPEDMDTPRTLYKINSTSPGTEAAAEAAAALSAASIAFKGVDSNYSVMLLKRSKLLFEFADKYRGSYQASCPFYCSYSGYQDELLWAAAWLYKASGETRYLNYVSSNQGWSQAVTEFSWDNKFVGAQTLLAKEFFDGTKDLGKFQTDADSFVCALMPGSSSVQIRTTPGGLLYTRDGNNLQYVTTSSMVLLIYSKTLAAAQIGGVQCGSAHFPTSQIRAFAKSQVDYILGNNPKKMSYMLGYGSNYPMQLHHRGASIPSIHALPDKVGCNQGYSSWYSSTEPNPNTHVGAIVGGPNSDDQFSDVRSDYSHSEPTTYANAAFVGSVAALVHKTREELPRADGAGEYNHRHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AHN1</accession>
    <name>A0AA39AHN1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_002414</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AHN1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9707389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="164" mass="18961" checksum="6B63A870FC034DC5" modified="2024-01-24" version="1">MIITEVCCSIHGDRRCCRSPPPLEFATSAIPEIIISHHHRHKRIIIFKGDQKYHCKVIVSREVEDEHTLLREEHSDVGDFDEVMKENLNNEAKFASGHEEHRACNAENSINCVRYQDAQSHDGLKEIDSREFPSIVALPLHFCRIIRALRRHKNISKIITTMLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AJU9</accession>
    <name>A0AA39AJU9_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_000794</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AJU9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47162:SF10">
        <property type="entry name" value="METHYL-CPG-BINDING DOMAIN-CONTAINING PROTEIN 9 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47162">
        <property type="entry name" value="OS02G0192300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041465748" evidence="1">
        <location>
            <begin position="23"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9708975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="218" mass="23974" checksum="C80A29C42E8630D3" modified="2024-01-24" version="1">MIRLLAFFFFFFGIDVLDDGDSGPVFKVQRFPCSMQPIVNASTVLLMLNFIQNDVKNKVGGDNSTSFGMDDDEDSGIQMIFSENDLPNLDYDVLSCSQNGLNEDCDGQTKSSSQTESNCLLQSSEKLVLSNLKVEDNIGEFLVEASSSSSTWNKISQTLVHACCEAYKQTGVLQFCCKHDLNRIWTPYAILNADVAASIGSLAKFCNFCGSINILCCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AK79</accession>
    <name>A0AA39AK79_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Asparagine synthetase domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_000028</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9707759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AK79"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004066">
        <property type="term" value="F:asparagine synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006529">
        <property type="term" value="P:asparagine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01991">
        <property type="entry name" value="Asn_synthase_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03766">
        <property type="entry name" value="Gn_AT_II_novel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001962">
        <property type="entry name" value="Asn_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051857">
        <property type="entry name" value="Asn_synthetase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45937">
        <property type="entry name" value="ASPARAGINE SYNTHETASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45937:SF1">
        <property type="entry name" value="ASPARAGINE SYNTHETASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00733">
        <property type="entry name" value="Asn_synthase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13537">
        <property type="entry name" value="GATase_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0061">Asparagine biosynthesis</keyword>
    <keyword evidence="3" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="5">
        <location>
            <begin position="99"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="Asparagine synthetase" evidence="4">
        <location>
            <begin position="354"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="Asparagine synthetase" evidence="4">
        <location>
            <begin position="516"/>
            <end position="593"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9707759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="645" mass="70965" checksum="EB0C814FDE9E51DD" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AMG6</accession>
    <name>A0AA39AMG6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Exocyst component Exo84 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_000899</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the EXO84 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AMG6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1210:FF:000002">
        <property type="entry name" value="Exocyst complex component EXO84B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1220:FF:000001">
        <property type="entry name" value="Exocyst complex component EXO84B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1220">
        <property type="entry name" value="Exo84p, C-terminal helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1210">
        <property type="entry name" value="Exo84p, N-terminal helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033961">
        <property type="entry name" value="Exo84"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032403">
        <property type="entry name" value="Exo84_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042561">
        <property type="entry name" value="Exo84_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042560">
        <property type="entry name" value="Exo84_C_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21426">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21426:SF12">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16528">
        <property type="entry name" value="Exo84_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08700">
        <property type="entry name" value="VPS51_Exo84_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst component Exo84 C-terminal" evidence="6">
        <location>
            <begin position="154"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="128"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="516"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="135"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF16528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9709153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="754" mass="83057" checksum="067FC983E32F3728" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D479</accession>
    <name>A0AA39D479_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Casein kinase II subunit alpha</fullName>
            <shortName evidence="9">CK II alpha</shortName>
            <ecNumber evidence="9">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_026823</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Casein kinases are operationally defined by their preferential utilization of acidic proteins as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6 9">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="9"/>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D479"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005956">
        <property type="term" value="C:protein kinase CK2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14132">
        <property type="entry name" value="STKc_CK2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000059">
        <property type="entry name" value="Casein kinase II subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000088">
        <property type="entry name" value="Casein kinase II subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045216">
        <property type="entry name" value="CK2_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24054">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24054:SF59">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT ALPHA-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="2 9" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041276220" description="Casein kinase II subunit alpha" evidence="10">
        <location>
            <begin position="20"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="106"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU369118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9670526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="399" mass="46269" checksum="E6D20C202C329D0F" modified="2024-01-24" version="1">MKDAHAPLVSLFPLLLVCALVALRAPLALPPNLRPHSSPPIPNNKIITTIVDTTKARLKLQLSEISSSDVAPMSKARVYADVNVLRPKDYWDYESLTVQWGDQDDYEVVRKVGRGKYSEVFEGINVNSNERCIIKILKPVKKKKIKREIKILQNLCGGPNIVKLLDIVRDQHSKTPSLIFEYVNSTDFKVLYPTLTDYDIRYYIYELLKALDYCHSQGIMHRDVKPHNVMIDHELRKLRLIDWGLAEFYHPGKEYNVRVASRYFKGPELLVDLQDYDYSLDMWSLGCMFAGMIFRKEPFFYGHDNHDQLVKIAKVLGTDELNAYLNKYRLELDPQLDALVGRHSRKPWSKFIHADNQHLVSPEAIDFLDKLLRYDHQDRLTAREAMQDFCQVKGLEAKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D7G9</accession>
    <name>A0AA39D7G9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_025609</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ubiquitin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D7G9"/>
    <dbReference type="SMR" id="A0AA39D7G9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01803">
        <property type="entry name" value="Ubl_ubiquitin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000016">
        <property type="entry name" value="Polyubiquitin 3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019954">
        <property type="entry name" value="Ubiquitin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019956">
        <property type="entry name" value="Ubiquitin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050158">
        <property type="entry name" value="Ubiquitin_ubiquitin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10666">
        <property type="entry name" value="UBIQUITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00348">
        <property type="entry name" value="UBIQUITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00299">
        <property type="entry name" value="UBIQUITIN_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="7">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="7">
        <location>
            <begin position="77"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="7">
        <location>
            <begin position="153"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="7">
        <location>
            <begin position="229"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="7">
        <location>
            <begin position="305"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="7">
        <location>
            <begin position="381"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9672037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="457" mass="51206" checksum="A1BF346A29F15F43" modified="2024-01-24" version="1">MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D7J5</accession>
    <name>A0AA39D7J5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="1">2.3.2.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_024003</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="1"/>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D7J5"/>
    <dbReference type="SMR" id="A0AA39D7J5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd23808">
        <property type="entry name" value="UBCc_UBE2W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000032">
        <property type="entry name" value="probable ubiquitin-conjugating enzyme E2 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="9">
        <location>
            <begin position="15"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="7">
        <location>
            <position position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00056190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9674813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="161" mass="18315" checksum="036588FAAA59F9A9" modified="2024-01-24" version="1">MTSSSAPSRKVLSKIACNRLQKELVEWQVNPPAGFKHKVTDNLQRWVIEVNGAPGTLYANESYQLQVDFPENYPMEAPQVVFVPPAPLHPHIYSNGHICLDILYDSWSPAMTVSSICISILSMLSSSTVKQRPADNDRYVKNCRNGRSPKETRWWFHDDKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D8M6</accession>
    <name>A0AA39D8M6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Glycosyltransferase subfamily 4-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_023637</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D8M6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000030">
        <property type="term" value="F:mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03816">
        <property type="entry name" value="GT33_ALG1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000109">
        <property type="entry name" value="Chitobiosyldiphosphodolichol beta-mannosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000083">
        <property type="entry name" value="UDP-glycosyltransferase TURAN isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026051">
        <property type="entry name" value="ALG1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13036">
        <property type="entry name" value="BETA1,4 MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13036:SF0">
        <property type="entry name" value="CHITOBIOSYLDIPHOSPHODOLICHOL BETA-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13692">
        <property type="entry name" value="Glyco_trans_1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13579">
        <property type="entry name" value="Glyco_trans_4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase subfamily 4-like N-terminal" evidence="10">
        <location>
            <begin position="39"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9674200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="484" mass="54948" checksum="1B5D77F0CB0371C0" modified="2024-01-24" version="1">MSGRKEENIGRRGRAAVVVLGDIGRSPRMQYHALSLARQASLEVDIVAYGGSTPHSAVLENQSIHIHTMKQWPTIPRIVSKIFYPLVLLFKALFQFFTLFWFLCVKIPSPDVFIVQNPPSVPTLVAVKWASWLRNSAFIVDWHNFGYTLLGLSLGRSSRFVALYHWFEKYYGKAANGSLCVTRAMQHELAQNWGIKATVLYDQPPEFFRPTPMEEKHKLFCRLHKDLCHPRSGQDCVTAGTMELWNQDTDETLFTAKMDTDIFLKSNRPALVVSSTSWTPDEDFSMLLEAAVMYDRRVAAILNEDDSTKEEVLWKETNSGKQFLYPRLLFIITGKGPNKEKYEEKIRRLKLNRVAFRTMWLSAEDYPLLLGSADLGICLHTSSSGLDLPMKVVDMFGCGLPVCAVSYSCIEELVKVEKNGLLFSSSSELANELLMLFKGFPDNCDALKLLRNGVVEAGFSARWDTEWERHAKPLISKVISEHCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D9U2</accession>
    <name>A0AA39D9U2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PIG-P domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_025025</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9676301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D9U2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013717">
        <property type="entry name" value="PIG-P"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47681">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL N-ACETYLGLUCOSAMINYLTRANSFERASE SUBUNIT P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47681:SF3">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL N-ACETYLGLUCOSAMINYLTRANSFERASE SUBUNIT P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08510">
        <property type="entry name" value="PIG-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="61"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="103"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="PIG-P" evidence="7">
        <location>
            <begin position="59"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9676301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="178" mass="19906" checksum="E50926E573188B03" modified="2024-01-24" version="1">MGWRPESLKEEDMEDRHSVSSPRRILSLSKQRRATVSFPDPDDKSSGFGVAGEHGPKPAEVYGFVGSISTVVATVIFLVWAYVPEHWLHSIGIFYYPNRQWALAVPAYAMVTVVLALGFYIGLNFMATPSPTSLNTMFDEYSREPMSFEPSIGDEQPIEPIADIGIDRINDLMFSDVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DCD4</accession>
    <name>A0AA39DCD4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_020691</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DCD4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19446">
        <property type="entry name" value="REVERSE TRANSCRIPTASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="235"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9678594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="908" mass="104312" checksum="20EC3F59429A3A57" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DDD7</accession>
    <name>A0AA39DDD7_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_021730</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DDD7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9679916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="69" mass="6459" checksum="B7D5927D23AB22FB" modified="2024-01-24" version="1">MAALTSVGVGANPSAGAGAVSTTTWNARDTGGGAASSPGLGGGPMTGATANDVRLAAIFGFHGLLRISS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DEE1</accession>
    <name>A0AA39DEE1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_020065</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DEE1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9680966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="58" mass="6471" checksum="A89347D9C3E6E2D2" modified="2024-01-24" version="1">MPDNPEPMTFGTDFFLMVQDMIKTEVRNYMSGLEINGICSQSEGNIRNAAINRIGMGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DEG2</accession>
    <name>A0AA39DEG2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transcription factor CBF/NF-Y/archaeal histone domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_018028</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NFYB/HAP3 subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9681938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DEG2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016602">
        <property type="term" value="C:CCAAT-binding factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009738">
        <property type="term" value="P:abscisic acid-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22907">
        <property type="entry name" value="HFD_NFYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000049">
        <property type="entry name" value="Nuclear transcription factor Y subunit B-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027113">
        <property type="entry name" value="Transc_fact_NFYB/HAP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003956">
        <property type="entry name" value="Transcrpt_fac_NFYB/HAP3_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11064">
        <property type="entry name" value="CCAAT-BINDING TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11064:SF176">
        <property type="entry name" value="TRANSCRIPTION FACTOR CBF_NF-Y_ARCHAEAL HISTONE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00615">
        <property type="entry name" value="CCAATSUBUNTA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00685">
        <property type="entry name" value="NFYB_HAP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0938">Abscisic acid signaling pathway</keyword>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription factor CBF/NF-Y/archaeal histone" evidence="9">
        <location>
            <begin position="9"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9681938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="147" mass="16504" checksum="CC4D134722EFE18E" modified="2024-01-24" version="1">MVDEQDHLLPIANVGRIMKQILPPRAKISKEAKETMQECASEFISFVTGEASDKCQKENRKTVNGDDICWALSALGFDDYAEAILRYLHKYREFERERANQNKVGGSQDKDEASNCKCIQAGKQSVIPPPLECRVIEKGNSSLTRPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DFB8</accession>
    <name>A0AA39DFB8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phosphatidic acid phosphatase type 2/haloperoxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_018698</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PA-phosphatase related phosphoesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DFB8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008195">
        <property type="term" value="F:phosphatidate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046839">
        <property type="term" value="P:phospholipid dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03390">
        <property type="entry name" value="PAP2_containing_1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.144.10:FF:000001">
        <property type="entry name" value="Lipid phosphate phosphatase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043216">
        <property type="entry name" value="PAP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165">
        <property type="entry name" value="LIPID PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165:SF203">
        <property type="entry name" value="LIPID PHOSPHATE PHOSPHATASE 3, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="66"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="166"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="194"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="225"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="8">
        <location>
            <begin position="98"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9682823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="313" mass="35819" checksum="6E8825CD58C55B72" modified="2024-01-24" version="1">MRQVQLGSHTVRSHGYTVARTHMHDWLILILLLVMVIVLNAIHPFYRFVGKDMMVDLKYPFKNNTVPIWAVPLYSVLLPITIFLIIYFRRRDVYDLHHAILGLLFSVLVTVVITDAIKNAVGRPRPDFFWRCFPDGKDLYDQWGNVICHGEKDIIKDGHKSFPSGHTAWSFSGLGFLSLYLSGKIRAFDNRGHVAKLCIVFLPLLVATLVGISRVDDYRHHWQDVFAGGLLGLIVATFFYLHFFPPPYDAEGWGPYAYFRTLEESRAQTQAANAVNVQDVEAQVENQQDERNRNTFIGLSLAHDSSSRQEENV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DII4</accession>
    <name>A0AA39DII4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">glucose-6-phosphate 1-epimerase</fullName>
            <ecNumber evidence="3 5">5.1.3.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_019090</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>alpha-D-glucose 6-phosphate = beta-D-glucose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:16249"/>
            <dbReference type="ChEBI" id="CHEBI:58225"/>
            <dbReference type="ChEBI" id="CHEBI:58247"/>
            <dbReference type="EC" id="5.1.3.15"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the glucose-6-phosphate 1-epimerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.15" evidence="3 5"/>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DII4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047938">
        <property type="term" value="F:glucose-6-phosphate 1-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09020">
        <property type="entry name" value="D-hex-6-P-epi_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008183">
        <property type="entry name" value="Aldose_1/G6P_1-epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025532">
        <property type="entry name" value="G6P_1-epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014718">
        <property type="entry name" value="GH-type_carb-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11122">
        <property type="entry name" value="APOSPORY-ASSOCIATED PROTEIN C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11122:SF33">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01263">
        <property type="entry name" value="Aldose_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016020">
        <property type="entry name" value="PHexose_mutarotase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR016020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR016020-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9683362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="306" mass="34682" checksum="5BE081FCDBFBF125" modified="2024-01-24" version="1">MPSNIVHGTDGFPRVILTDTTGSSAEVLLYGGQVVSWKNERREELLFMSTKAVRKPPKAIRGGIPVCFPEFGNFGSLEQHGFARNRLWSLDSNPSPLSPSNSQSSVDLILKSTQQDVRTWPWRFELRLRISLSDGKLTLIPRVRNTDSKPFSFMFALRNYLSVSDVSEVRVEGLETLDYFDNLMQRERHTEQADAITFDGEIDRVYLCTPTKIAIIDHEKKRTIVLRKEGMPDAAVWNPWDKKAKALPDMGDEDYETMLCVDSAAIETPIVLKPFEEWRGRQELSTVSSSYCSGQLDPRKVLHGLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DIZ2</accession>
    <name>A0AA39DIZ2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Plant/F9H3-4 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_015476</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DIZ2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008507">
        <property type="entry name" value="DUF789"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31343:SF3">
        <property type="entry name" value="DUF789 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31343">
        <property type="entry name" value="T15D22.8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05623">
        <property type="entry name" value="DUF789"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="102"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9686618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="318" mass="36659" checksum="85C76021685C6CDC" modified="2024-01-24" version="1">MILDKGSMQSNLQCFLECTTPVVQSQFLPKTEIRDLNVLWHPWEREKVEYFTLGDLWNCFDEWSAYGAGVPILLNNNETLVQYYVPYLSAIQIFTSNPARNSFREETESGDCETRDSFSDSCSDESESEKQWRSDGCSSEEGCLEQESLWHRSDKLGCLYFQYFERSTPYGRVPLMDKISGLAQRYPGLMSLRSVDLSPASWMAVAWYPIYHIPMGRTIKDLSTCFLTYHTLSSSFQDMDLDDDIESAEKKKRKEGEGISLPPFGLATYKMQGNVWVSSESGRDQERVMSLLSVADSWLKQLRVQHHDFNYFTGIRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DIZ7</accession>
    <name>A0AA39DIZ7_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_015485</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DIZ7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9686633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="111" mass="12410" checksum="618FB9FB4B3B226E" modified="2024-01-24" version="1">MPAAGELVQDGLHLGAILLPAEKQLFHHVAHAAAADAEHHHRIAGALPSHRLVRGLRLPLGFTVHSRLFLYLAWFVHWHASWFRTAMKRVAVFVVFCSSSTNRPYLTAARC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DJ73</accession>
    <name>A0AA39DJ73_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MO25-like protein At5g47540</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_015629</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Mo25 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DJ73"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043539">
        <property type="term" value="F:protein serine/threonine kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000146">
        <property type="entry name" value="putative MO25-like protein At5g47540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013878">
        <property type="entry name" value="Mo25"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10182">
        <property type="entry name" value="CALCIUM-BINDING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10182:SF34">
        <property type="entry name" value="MO25-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08569">
        <property type="entry name" value="Mo25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9686858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="347" mass="40272" checksum="9E88846255FC7A52" modified="2024-01-24" version="1">MKGLFKSKPRTPAELVRYMRELLIFIDRGAQTREQKREKQREEKVSELNKCILEIRTILYGIDGAEPVSEACAQLTLEFLKEDTIRLLIVCLPKLDLGARQNATRVITNLSRQRVNSRLIAAEYLENNIDVINILIPGYEDSEIALSYGAILREYIRYQIVARYVLESAQMKKFFDYVQNPTFEIASDAAATFKELLTRHKSTVAEFLSKNFDWFFREYNSQLLQSPNYITRRHAVKLLGDMLLDRSNSAVMIRYVSSLDNMRILMNLLRESNKAIQLESFHVFKLFVANQNKPPEIVSILVTNRNKLLRFFGDFNIDKEDDQFEADKAQVIREITNLNPQADDGVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DL70</accession>
    <name>A0AA39DL70_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Auxin-responsive protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_014272</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Aux/IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimers and heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Aux/IAA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DL70"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000078">
        <property type="entry name" value="Auxin-responsive protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033389">
        <property type="entry name" value="AUX/IAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003311">
        <property type="entry name" value="AUX_IAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31734:SF34">
        <property type="entry name" value="AUXIN-RESPONSIVE PROTEIN IAA15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31734">
        <property type="entry name" value="AUXIN-RESPONSIVE PROTEIN IAA17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02309">
        <property type="entry name" value="AUX_IAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0927">Auxin signaling pathway</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0678">Repressor</keyword>
    <keyword evidence="5 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="PB1" evidence="10">
        <location>
            <begin position="114"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="9"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9688526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="224" mass="24552" checksum="EB6A021C12607DDD" modified="2024-01-24" version="1">MQDFKFDTMPSNTADQSPDSGTTAMSLKDTELTLGLPGGAQVVIVGGKSCSKRGYTDTVDFRSRCCSGESSAKAEQVDWPGNEISGPGNAPDSKAQVVGWPPVRSGRKKALKSCKYVKVAVDGAPYLRKVDLEVHRSYQQLLMALETMFDCFTIRRNDLEESKIMNPVNGAEYVPTYEDKDGDWMLVGDVPWNMFVESCKRVRLMKSSEAIGLAPRTPSCTSTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DLA2</accession>
    <name>A0AA39DLA2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_014338</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DLA2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016628">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000121">
        <property type="entry name" value="Prostaglandin reductase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041694">
        <property type="entry name" value="ADH_N_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045010">
        <property type="entry name" value="MDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43205:SF12">
        <property type="entry name" value="OS06G0602900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43205">
        <property type="entry name" value="PROSTAGLANDIN REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16884">
        <property type="entry name" value="ADH_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="2">
        <location>
            <begin position="18"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9688631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="348" mass="37839" checksum="724981CEE5D7CA3B" modified="2024-01-24" version="1">MGREVINKYVTIKTHIDGAPQDSDFELKTAALRLWAEPGSNDVIVKNLHVSIDPYQLNRMKGYSSSHRTINFAVPISPGMDIDAYGVGRVVASGHPEFEEDDLVVGLISWGEYSVVKGGNTLRKLDPMGFPLSNHVGILGFSGLAAHGGFFQVCKPKKGEKVFVSAASGSVGNLVGQYAKLFGCYVVGCAGSKQKVNLLKEKLGFDDAFNYKEETDLKSTLKRYFPDGIDIYFDNVGAEMLEAAVANMNIFGRVAVCGVISQYTDSGKRAAPDMLDIVYKRITIQGFLAADLMNGYTDFISTTQDYLNDGKIQVIEDISQGVESIPSAFVGLFRGDNVGKKIVKIADE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DNK7</accession>
    <name>A0AA39DNK7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Toprim domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_013500</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DNK7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01029">
        <property type="entry name" value="TOPRIM_primases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1360.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034154">
        <property type="entry name" value="TOPRIM_DnaG/twinkle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027032">
        <property type="entry name" value="Twinkle-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12873">
        <property type="entry name" value="T7-LIKE MITOCHONDRIAL DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12873:SF6">
        <property type="entry name" value="TOPRIM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13662">
        <property type="entry name" value="Toprim_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56731">
        <property type="entry name" value="DNA primase core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Toprim" evidence="1">
        <location>
            <begin position="243"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9691343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="380" mass="42848" checksum="04B58691B56F153D" modified="2024-01-24" version="1">MPQLLAALLPSKQWLKSTFWLQHFLSFASPPKPVFLFQSTPSGFRFNSYAATRRVSTIPVRLDQPEDGSSDLDKLRVLRQKIEVSGITLDDSCVPGRYHHLICPKCNGGHLMERSLSLHITQDRNFAMWRCFRSTCGWAGRVFPESSAAYSEVTNNWMTVDSLGLEPLGDKLIAYFGERMISEKTLCRNAVLQLSGNQSVIAFTYRQNGLLVGCKYRSMGKRFWQEKGTEKILYGLDDIQEANEIIIVEGEVDKLSVEEAGFCNCVSVPGGAPQKVSAKELPSLDKDTAYHYLWNCKEYLDKASRIILATDGDSPGRALAEELARRLGKERCWRVSWPKKEDSSCFKDANEVLKNLGPDALREVIENAELYEVNSSIQEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DSU0</accession>
    <name>A0AA39DSU0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Disease resistance protein RGA3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_011839</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DSU0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14798">
        <property type="entry name" value="RX-CC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038005">
        <property type="entry name" value="RX-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766:SF45">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766">
        <property type="entry name" value="PLANT BROAD-SPECTRUM MILDEW RESISTANCE PROTEIN RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="6">
        <location>
            <begin position="6"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="5">
        <location>
            <begin position="182"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="7">
        <location>
            <begin position="433"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="8">
        <location>
            <begin position="573"/>
            <end position="876"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9692917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="914" mass="105096" checksum="C479AD903D9B41AA" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DT77</accession>
    <name>A0AA39DT77_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Proline-rich protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_008670</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DT77"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33470">
        <property type="entry name" value="OS01G0164075 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33470:SF40">
        <property type="entry name" value="PROTEIN SEED AND ROOT HAIR PROTECTIVE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01190">
        <property type="entry name" value="Pollen_Ole_e_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041457003" description="Proline-rich protein 3" evidence="2">
        <location>
            <begin position="23"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9696566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="179" mass="19713" checksum="8B2C0ECB7656FE0D" modified="2024-01-24" version="1">MALTHFFTILMVLFSLVAITSASSGGYGPEPEVKKSDEPEYNEKLPTIMGVQGLIYCKSGPKPMPLPGAVARITCLKVNKDGYEMGSYSFLSGASDEKGYFFATLYPSEVEDDCKLKVCKAFLETSPLETCDVPTDDNNGISGYPLDFYRSLSAKKMMLYSVKPFFYTPKPEPPISNGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DUB3</accession>
    <name>A0AA39DUB3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Enoyl-[acyl-carrier-protein] reductase [NADH], chloroplastic</fullName>
            <ecNumber evidence="5">1.3.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_007129</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes the NAD-dependent reduction of a carbon-carbon double bond in an enoyl moiety that is covalently linked to an acyl carrier protein (ACP). Catalyzes the last reduction step in the de novo synthesis cycle of fatty acids. Involved in the elongation cycle of fatty acids which are used in lipid metabolism. Required for normal plant growth.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a 2,3-saturated acyl-[ACP] + NAD(+) = a (2E)-enoyl-[ACP] + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10240"/>
            <dbReference type="Rhea" id="RHEA-COMP:9925"/>
            <dbReference type="Rhea" id="RHEA-COMP:9926"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:78784"/>
            <dbReference type="ChEBI" id="CHEBI:78785"/>
            <dbReference type="EC" id="1.3.1.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.9" evidence="5"/>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9697856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DUB3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004318">
        <property type="term" value="F:enoyl-[acyl-carrier-protein] reductase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05372">
        <property type="entry name" value="ENR_SDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.400:FF:000001">
        <property type="entry name" value="Enoyl-[acyl-carrier-protein] reductase [NADH]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000192">
        <property type="entry name" value="Enoyl-[acyl-carrier-protein] reductase [NADH]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.400">
        <property type="entry name" value="Enoyl acyl carrier protein reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014358">
        <property type="entry name" value="Enoyl-ACP_Rdtase_NADH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004957">
        <property type="entry name" value="PRK06300.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43159">
        <property type="entry name" value="ENOYL-[ACYL-CARRIER-PROTEIN] REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43159:SF2">
        <property type="entry name" value="ENOYL-[ACYL-CARRIER-PROTEIN] REDUCTASE [NADH], CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="13" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="6" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-0934">Plastid</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00053870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00067308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9697856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="392" mass="41722" checksum="5018C6677FC8C396" modified="2024-01-24" version="1">MAATAVSGLQMATARSHISSSHRILKAGAAVLGANSKGRSWAKLASACHISYFHHFQRSFSSSPIRFDKVVTRAMSEASEKKPTSGLPIDLKGKRAFIAGVADDNGYGWAIAKSLAAAGAEILVGTWVPALNIFETSLRRGKFDESRVLPDGSLMEITKIYPLDAVYDTLEDVPEDVKANKRYAGSSNWTVQEVAESVKQDFGSIDILVHSLANGPEVTKPLLETSRKGYLVAISASSYSYVSLLKYFIPIMNPGGSSISLTYIASERIIPGYGGGMSSAKAALESDTRVLAFEAGRKHKIRVNTISAGPLRSRAAKAIGFIDTMIEYSLANAPLQKELSADEVGNTAAFLASPLASAITGAVIYVDNGLNAMGVGVDSPMFADLNIPKDKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DYD1</accession>
    <name>A0AA39DYD1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_007504</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the GRAS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DYD1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005202">
        <property type="entry name" value="TF_GRAS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31636">
        <property type="entry name" value="OSJNBA0084A10.13 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03514">
        <property type="entry name" value="GRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50985">
        <property type="entry name" value="GRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="193"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="260"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="VHIID" evidence="3">
        <location>
            <begin position="350"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Leucine repeat II (LRII)" evidence="3">
        <location>
            <begin position="431"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="SAW" evidence="3">
        <location>
            <begin position="569"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="VHIID" evidence="3">
        <location>
            <begin position="381"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="45"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9698452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="649" mass="73338" checksum="93A285B21B0243D1" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DZ21</accession>
    <name>A0AA39DZ21_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_006139</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DZ21"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041224017" evidence="2">
        <location>
            <begin position="16"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9700675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="96" mass="10137" checksum="EA8D5B22D035487A" modified="2024-01-24" version="1">MACLVVVASLPGVVCQMDLVACVRGVASLHWEGAQGCDHNEVASLPLVVCQIGWLAFYGFCQMACVLVVASLLGVVFCQMDLVACVRGVAPLPWEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E113</accession>
    <name>A0AA39E113_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">cysteine--tRNA ligase</fullName>
            <ecNumber evidence="5">6.1.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Cysteinyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PVL29_004444</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Plastid</location>
            <location evidence="3">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.16" evidence="5"/>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E113"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004817">
        <property type="term" value="F:cysteine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006423">
        <property type="term" value="P:cysteinyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00672">
        <property type="entry name" value="CysRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000009">
        <property type="entry name" value="Cysteine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1910:FF:000003">
        <property type="entry name" value="Cysteine--tRNA ligase CPS1, chloroplastic/mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1910">
        <property type="entry name" value="Cysteine-tRNA ligase, C-terminal anti-codon recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00041">
        <property type="entry name" value="Cys_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015803">
        <property type="entry name" value="Cys-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015273">
        <property type="entry name" value="Cys-tRNA-synt_Ia_DALR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024909">
        <property type="entry name" value="Cys-tRNA/MSH_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056411">
        <property type="entry name" value="CysS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032678">
        <property type="entry name" value="tRNA-synt_1_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00435">
        <property type="entry name" value="cysS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10890:SF25">
        <property type="entry name" value="CYSTEINE--TRNA LIGASE, CHLOROPLASTIC_MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10890">
        <property type="entry name" value="CYSTEINYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23493">
        <property type="entry name" value="CysS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01406">
        <property type="entry name" value="tRNA-synt_1e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00983">
        <property type="entry name" value="TRNASYNTHCYS"/>
    </dbReference>
    <dbReference type="SMART" id="SM00840">
        <property type="entry name" value="DALR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="7" id="KW-0436">Ligase</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0934">Plastid</keyword>
    <keyword evidence="13" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Cysteinyl-tRNA synthetase class Ia DALR" evidence="18">
        <location>
            <begin position="427"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00031499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ9702715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="580" mass="66063" checksum="ED46AD86C89B92CE" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E387</accession>
    <name>A0AA39E387_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_001945</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E387"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11073">
        <property type="entry name" value="CYP76-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000007">
        <property type="entry name" value="Cytochrome P450 76C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47950">
        <property type="entry name" value="CYTOCHROME P450, FAMILY 76, SUBFAMILY C, POLYPEPTIDE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47950:SF4">
        <property type="entry name" value="GERANIOL 8-HYDROXYLASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7 8" id="KW-0349">Heme</keyword>
    <keyword evidence="5 7 8" id="KW-0408">Iron</keyword>
    <keyword evidence="3 7 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 8" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="7">
        <location>
            <position position="442"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9706718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="499" mass="56192" checksum="76D494C0BC41D25B" modified="2024-01-24" version="1">MELLSCLLCFLVAWTSIYILLSVRRGSKHTAYKLPPGPVPLPIIGNLLNLGNRPHESLAILAKTYGPIMTLKLGYVTTIVISSAPMAKEVLQKQDLSFCNRSIPDAIRAADHNQLSMAWMPVSTTWRALRRTCNSHLFTSQKLDSNTHLRHQKVQELLANVEQSCRAGGPVDIGREAFRTSLNLLSNTIFSVDLVDPISETAQEFKELVRGVMDEAGKPNLVDYFPVLRQIDPQGIRRRLTIYFGRMIEIFDRMIKQRLQLRKIQGSIASSDVLDVLLNISEDNSNEIERSHMEHLLLDLFAAGTDTTSSTLEWAMAELLHNPETLLKARMELLQTIGQDKQVKESDITRLPYLQAVVKETLRLHPAIPFLLPRRVEGDADIDGFAVPKNAQVLVNAWAIGRDPNTWENPNSFVPERFLGLDMDVKGQNFELIPFGAGRRICPGLPLAIRMVHLMLASLIHSYDWKLEDGVTPENMNMEERYGISLQKAQPLQALPVRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E3F6</accession>
    <name>A0AA39E3F6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_003323</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E3F6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000024">
        <property type="entry name" value="Somatic embryogenesis receptor kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47988">
        <property type="entry name" value="SOMATIC EMBRYOGENESIS RECEPTOR KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041290144" description="Leucine-rich repeat-containing N-terminal plant-type domain-containing protein" evidence="4">
        <location>
            <begin position="24"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="5">
        <location>
            <begin position="25"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="6">
        <location>
            <begin position="72"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9705205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="213" mass="23674" checksum="C40B604C16881443" modified="2024-01-24" version="1">MDSSTSVLCFLLPVLLSLTSTLSTNSEGNALHALRSRLSDPTNVLQSWDPTLVNPCTWFHVTCDSNNRVIRLDLGNSNVSGSLGPELGQLQHLQYLELYRNNIDGKIPKELGNLKNLISMDLYDNKFEGKIPKSIAKLKSLRFLRLNNNKLTGSIPRELTTLSNLKVFDVSNNNLCGTIPVDGPFVNFPMESFQNNRFSGPELQGLVPYDFGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E3L1</accession>
    <name>A0AA39E3L1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_005208</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E3L1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04928">
        <property type="entry name" value="ACT_TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13999">
        <property type="entry name" value="STKc_MAP3K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000060">
        <property type="entry name" value="Serine/threonine-protein kinase isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF151">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE STY17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01842">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ACT" evidence="12">
        <location>
            <begin position="181"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="296"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="112"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="8"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="127"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9703837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="577" mass="65333" checksum="6467567F36E3099F" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E416</accession>
    <name>A0AA39E416_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Metal-nicotianamine transporter YSL1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_001716</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the YSL (TC 2.A.67.2) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E416"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051980">
        <property type="term" value="F:iron-nicotianamine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035673">
        <property type="term" value="F:oligopeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010039">
        <property type="term" value="P:response to iron ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048316">
        <property type="term" value="P:seed development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004813">
        <property type="entry name" value="OPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045035">
        <property type="entry name" value="YSL-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00728">
        <property type="entry name" value="OPT_sfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31645:SF11">
        <property type="entry name" value="METAL-NICOTIANAMINE TRANSPORTER YSL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31645">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER YGL114W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03169">
        <property type="entry name" value="OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="72"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="157"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="281"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="319"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="383"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="412"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="463"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="504"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="595"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="631"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9706329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="668" mass="73912" checksum="CE21BA0F09DFCDFF" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E5U4</accession>
    <name>A0AA39E5U4_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_002566</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E5U4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9707590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="117" mass="13118" checksum="3E15D9B792828560" modified="2024-01-24" version="1">MDSSDIMELVENEEVFSGFVDLKSQELHGVCDGKLSEKELEPVGTSENKRMSWMMRIGKLRLFQSSMLLIGKDGLRTFKIGVSQGNFGGSLLKPMCLTPVFSCMLSKSDFQKNLVFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YHK5</accession>
    <name>A0AA38YHK5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_026871</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YHK5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050651">
        <property type="entry name" value="Plant_Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47947:SF26">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47947">
        <property type="entry name" value="CYTOCHROME P450 82C3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0349">Heme</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9670588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="288" mass="33545" checksum="F4C8369745DEA86D" modified="2024-01-24" version="1">MFIGTRRDEIKILLHRLSQNSRDNFARVELRLMFTEPTCNIVMRMVAGKRYYGEDVDFEEAKHFHEVMRGVFELAGVSNPGEFFPLLRWIKTKKEVIFQGLIDEHRSSNRGLVNKNSMINHLLSMQKSEPEYYTDEIIKAHSLILILVGTETAATTIEWAMSLLLNHPEVMKKARVVELDTHVGKERLMEESNFPKLQYLQSTISETLRLFPAAPVLVPHMSSDNCQIGGNIQRDTILLVNAWAIHRDPKLWKDATSFKPERFENRESETYKLAIWIEKKGLPWYWPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YIQ2</accession>
    <name>A0AA38YIQ2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Hexosyltransferase</fullName>
            <ecNumber evidence="13">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_027242</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Golgi apparatus membrane</location>
            <topology evidence="2 13">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the glycosyltransferase 31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="13"/>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9671163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YIQ2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048531">
        <property type="term" value="F:beta-1,3-galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.50:FF:000002">
        <property type="entry name" value="Hexosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025298">
        <property type="entry name" value="DUF4094"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002659">
        <property type="entry name" value="Glyco_trans_31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214:SF226">
        <property type="entry name" value="BETA-1,3-GALACTOSYLTRANSFERASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214">
        <property type="entry name" value="BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13334">
        <property type="entry name" value="DUF4094"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01762">
        <property type="entry name" value="Galactosyl_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10 13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12 13" id="KW-0464">Manganese</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="DUF4094" evidence="14">
        <location>
            <begin position="12"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF13334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9671163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="430" mass="49046" checksum="7FE8ED7DBDDE266A" modified="2024-01-24" version="1">MKSRNNSEKFSPTWIFILCIFSFALGMLFTNRMWAAPESNRQILSNQRHEQELQIISEDCTSKKVGQEKDVMGEVYKTHEAIQSLDKTISTLQIELSATRTSHKTGSLESSPDVMRSSHDSFPRKKAFMVIGINTAFSSRKRRDSIRETWMPKGQKLLQLEREKGIVVRFMIGHSATSSSILDRAIDSEESQHKDFLRLEHVEGYHELTAKTKTFFSMAVAQWDAEFYVKVDDDVHVNLGMLASTLARHRSKPRVYIGCMKSGPVLSQKTVKYHEPEYWKFGEEGNKYFRHATGQIYAISKDLATYISVNQPILHKYANEDVSLGAWFIGLEVEHIDDRNLCCGTPPGTRFFHYILYSSFFHVKVFLPNGVLFGYLILVVSDCEWKAQAGNVCVASFDWSCSGICRSVERIKDVHARCGEGDGTIWSTPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YK81</accession>
    <name>A0AA38YK81_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Disease resistance protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_025571</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YK81"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057135">
        <property type="entry name" value="At4g27190-like_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050905">
        <property type="entry name" value="Plant_NBS-LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463:SF202">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23247">
        <property type="entry name" value="LRR_RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NB-ARC" evidence="7">
        <location>
            <begin position="163"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="9">
        <location>
            <begin position="410"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="10">
        <location>
            <begin position="554"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein At4g27190-like leucine-rich repeats" evidence="8">
        <location>
            <begin position="830"/>
            <end position="932"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9671985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="976" mass="109340" checksum="29391A8F9991F63B" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YK98</accession>
    <name>A0AA38YK98_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_025574</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YK98"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.430:FF:000003">
        <property type="entry name" value="Probable disease resistance protein At5g66910"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057135">
        <property type="entry name" value="At4g27190-like_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050905">
        <property type="entry name" value="Plant_NBS-LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463:SF202">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23247">
        <property type="entry name" value="LRR_RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="168"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9671988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="991" mass="111934" checksum="36394778B5236F35" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YKT0</accession>
    <name>A0AA38YKT0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SWIB complex BAF60b domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_025762</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YKT0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd10567">
        <property type="entry name" value="SWIB-MDM2_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.245.10">
        <property type="entry name" value="SWIB/MDM2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014876">
        <property type="entry name" value="DEK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019835">
        <property type="entry name" value="SWIB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036885">
        <property type="entry name" value="SWIB_MDM2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003121">
        <property type="entry name" value="SWIB_MDM2_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13844">
        <property type="entry name" value="SWI/SNF-RELATED MATRIX-ASSOCIATED ACTIN-DEPENDENT REGULATOR OF CHROMATIN SUBFAMILY D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08766">
        <property type="entry name" value="DEK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02201">
        <property type="entry name" value="SWIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00151">
        <property type="entry name" value="SWIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47592">
        <property type="entry name" value="SWIB/MDM2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51998">
        <property type="entry name" value="DEK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51925">
        <property type="entry name" value="SWIB_MDM2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEK-C" evidence="3">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="DM2" evidence="2">
        <location>
            <begin position="146"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="DM2" evidence="2">
        <location>
            <begin position="249"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9672273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="331" mass="36649" checksum="6400C11AEE33AF98" modified="2024-01-24" version="1">MVTDEAIAEALDSLIRESTPSQFTSINGVVLQLESKLGLNLSHKLDFIRSQIHLFFQSNQPQSQPPPPQPPSQQQPPKDHFTLQQATNFSTGPPNFAPHRTEDLNFRRDPTPAPPAEPTGSPVSVPEAPPQESARAGTKRRGGPGGLNKVCGVSPELQAIVGQPALPRTEIVKQLWAYIRRNNLQDPSNKRKIICNDELRLVFETDSTDMFKMNKLLAKHIIPLEPTKQSGEQSKKLKVDAGAGTKSSESGPYVVISEALANFFGTSGREMLQSEVLRRVWEYIKVNHLEDPLNSMVILCDAKLQEIFGCESISALGIPEILMRHHLCKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YKZ3</accession>
    <name>A0AA38YKZ3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Zinc transporter 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_025836</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YKZ3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040:SF217">
        <property type="entry name" value="ZINC TRANSPORTER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040">
        <property type="entry name" value="ZINC/IRON TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041351378" description="Zinc transporter 11" evidence="6">
        <location>
            <begin position="22"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="47"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="78"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="116"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="196"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="222"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="257"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="288"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="327"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9672372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="347" mass="36910" checksum="C54EDB3F6F921E7D" modified="2024-01-24" version="1">MSRSLLSLSLLLLVLAAVAAAHGGDHADDDDGDAPSSEGVDLRAKPLILVKIYCLILVFVGTFIGGVSPYFLKWNETFLVLGTQFAGGVFLGTAMMHFLSDSNETFGDLTSVEYPFAFMLACAGYLMTMFADCLVSYVYGKGANGGDGDVELQENVRGKSSSNGGNSESLAEDQNCTAVHFVNAPLKAATSFGDNILLIFALCFHSVFEGIAIGVAETKADAWRALWTVCLHKIFAAIAMGIALLRMIPDRPLLSCVAYAFAFAISSPIGVAIGIVIDATTQGAVADWIYAISMGLACGIFIYVSINHLLSKGYTCQRTVPFDTPNYKFLAVLLGVGVIAVVMIWDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YN27</accession>
    <name>A0AA38YN27_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="25">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">PVL29_023203</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="25">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Serine/threonine-protein kinase that may function as a signaling receptor of extracellular matrix component. Binding to pectin may have significance in the control of cell expansion, morphogenesis and development.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="19">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="25">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YN27"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000038">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000084">
        <property type="entry name" value="Wall-associated receptor kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000043">
        <property type="entry name" value="Wall-associated receptor kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.20.10">
        <property type="entry name" value="Plasmodium vivax P25 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013695">
        <property type="entry name" value="WAK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045274">
        <property type="entry name" value="WAK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025287">
        <property type="entry name" value="WAK_GUB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27005:SF468">
        <property type="entry name" value="OS01G0310500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27005">
        <property type="entry name" value="WALL-ASSOCIATED RECEPTOR KINASE-LIKE 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13947">
        <property type="entry name" value="GUB_WAK_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08488">
        <property type="entry name" value="WAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 19" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="13 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7 22" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041255589" evidence="22">
        <location>
            <begin position="30"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="341"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="24">
        <location>
            <begin position="290"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="416"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="445"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00058961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="KAJ9673500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="738" mass="82345" checksum="99CA3689A53327EA" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YNT6</accession>
    <name>A0AA38YNT6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4057 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_023392</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YNT6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025131">
        <property type="entry name" value="DUF4057"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31132">
        <property type="entry name" value="N-LYSINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31132:SF13">
        <property type="entry name" value="N-LYSINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13266">
        <property type="entry name" value="DUF4057"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4057" evidence="2">
        <location>
            <begin position="3"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="197"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="24"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="206"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9673813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="304" mass="32427" checksum="ED501DDF435683A2" modified="2024-01-24" version="1">MERATPVRKPHTSTADLLTWSETPPADSPASASRPAVRSHQPSDGISKVVFGGQVTDEEAEALSKRKPCSGYKLKEITGSGIFALDGENGTSESGSANPTPNSKTTVRTYQQTAAAISQISFGTEESVSPKKPTTLPEVAKQRELSGTLESEETKMQKQLSDAKCKELSGHDIFAPPPEILPRPLPAHSLVLKESKDMGEPAPRNVRTSVKVSNPAGGQSNIMFSDETVLKTAKKIHNQKFVELTGNDIFKGDVPPSSAEKSLSMAKLREMSGNDIFADGKAESRDYLGGVRKPPGGESSIALV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YNV3</accession>
    <name>A0AA38YNV3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein FAM91A1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_023452</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM91 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YNV3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039199">
        <property type="entry name" value="FAM91"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028097">
        <property type="entry name" value="FAM91_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028091">
        <property type="entry name" value="FAM91_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28441">
        <property type="entry name" value="PROTEIN FAM91A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28441:SF2">
        <property type="entry name" value="PROTEIN FAM91A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14648">
        <property type="entry name" value="FAM91_C"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14647">
        <property type="entry name" value="FAM91_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAM91 N-terminal" evidence="3">
        <location>
            <begin position="17"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="FAM91 C-terminal" evidence="4">
        <location>
            <begin position="368"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="FAM91 C-terminal" evidence="4">
        <location>
            <begin position="558"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="domain" description="FAM91 C-terminal" evidence="4">
        <location>
            <begin position="784"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="330"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="510"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="338"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="515"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="528"/>
            <end position="543"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9673911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="999" mass="111338" checksum="2C515606015EB5B4" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YQM8</accession>
    <name>A0AA38YQM8_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PVL29_024030</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YQM8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000101">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000192">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase RPK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000299">
        <property type="entry name" value="Piriformospora indica-insensitive protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000840">
        <property type="entry name" value="Probable inactive leucine-rich repeat receptor kinase XIAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000404">
        <property type="entry name" value="Putative LRR receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27000:SF642">
        <property type="entry name" value="INACTIVE LEUCINE-RICH REPEAT RECEPTOR KINASE XIAO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27000">
        <property type="entry name" value="LEUCINE-RICH REPEAT RECEPTOR-LIKE PROTEIN KINASE FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 15" id="KW-0732">Signal</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041262027" evidence="15">
        <location>
            <begin position="22"/>
            <end position="1131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="754"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="17">
        <location>
            <begin position="29"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="18">
        <location>
            <begin position="334"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="domain" description="Serine-threonine/tyrosine-protein kinase catalytic" evidence="16">
        <location>
            <begin position="840"/>
            <end position="1056"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="790"/>
            <end position="817"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF07714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ9674851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1131" mass="123139" checksum="A4EF1A4D9A251B04" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YRA7</accession>
    <name>A0AA38YRA7_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_024177</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YRA7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33133">
        <property type="entry name" value="OS08G0107100 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33133:SF51">
        <property type="entry name" value="THH1_TOM1_TOM3 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="143"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="180"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="269"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="336"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9675122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="378" mass="42886" checksum="4FD73DF5AA2DD536" modified="2024-01-24" version="1">MEVEHEEMKSLGVFGIYRKAYKIILPWRKIFTQIILAYILPLLFISLVNTHLSNPLLPKIVDQEKKDLAETQVPTSSHTNIFDLLSFPFSSYWLFQASYTIYSFIFSLVSTSAIVYTMACIYSGREVTFRMVTSVVPKVGRRLLLTSFTIFLVVCTYHVVAFLVFALAAVLIAFGPNTNVGMSILLVVVVLYLMGLLYMSVVWQLASTISVLEDSYGFQAMKRSNQLIKGKVGVSTLIFLKLGLLHYVLQKALERVVVNGESLGMVNRVAYANVCFSLFLLVGLFERVIQTIIYFVCKSYHHERVDKLALSDHLQVYSQEYSLLLKGDNLDELKQLGWAILLCVLMVSKVAIVCLSRLLFLRICLKSNIGLNYKEIFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YRS8</accession>
    <name>A0AA38YRS8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">Laccase</fullName>
            <ecNumber evidence="4 13">1.10.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Benzenediol:oxygen oxidoreductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Diphenol oxidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Urishiol oxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_024343</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Lignin degradation and detoxification of lignin-derived products.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>4 hydroquinone + O2 = 4 benzosemiquinone + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:11276"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17594"/>
            <dbReference type="ChEBI" id="CHEBI:17977"/>
            <dbReference type="EC" id="1.10.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="13">Binds 4 Cu cations per monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Secreted</location>
            <location evidence="2 13">Extracellular space</location>
            <location evidence="2 13">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the multicopper oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.10.3.2" evidence="4 13"/>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YRS8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052716">
        <property type="term" value="F:hydroquinone:oxygen oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046274">
        <property type="term" value="P:lignin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13849">
        <property type="entry name" value="CuRO_1_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13875">
        <property type="entry name" value="CuRO_2_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13897">
        <property type="entry name" value="CuRO_3_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033138">
        <property type="entry name" value="Cu_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002355">
        <property type="entry name" value="Cu_oxidase_Cu_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034288">
        <property type="entry name" value="CuRO_1_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034285">
        <property type="entry name" value="CuRO_2_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034289">
        <property type="entry name" value="CuRO_3_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017761">
        <property type="entry name" value="Laccase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03389">
        <property type="entry name" value="laccase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF487">
        <property type="entry name" value="LACCASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00079">
        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00080">
        <property type="entry name" value="MULTICOPPER_OXIDASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0052">Apoplast</keyword>
    <keyword evidence="10 13" id="KW-0186">Copper</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 13" id="KW-0439">Lignin degradation</keyword>
    <keyword evidence="7 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 13" id="KW-0964">Secreted</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041485401" description="Laccase" evidence="13">
        <location>
            <begin position="25"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="16">
        <location>
            <begin position="35"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="14">
        <location>
            <begin position="162"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="15">
        <location>
            <begin position="422"/>
            <end position="552"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9675357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="569" mass="63517" checksum="EE5DF61FD30066EB" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YSH2</accession>
    <name>A0AA38YSH2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_024632</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YSH2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025724">
        <property type="entry name" value="GAG-pre-integrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054722">
        <property type="entry name" value="PolX-like_BBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039537">
        <property type="entry name" value="Retrotran_Ty1/copia-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648:SF20">
        <property type="entry name" value="RNA-DIRECTED DNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648">
        <property type="entry name" value="TRANSPOSASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13976">
        <property type="entry name" value="gag_pre-integrs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22936">
        <property type="entry name" value="Pol_BBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="6">
        <location>
            <begin position="466"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="196"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="700"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="196"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="216"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="227"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9675785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1252" mass="142787" checksum="FD9A4116D1FA6EC9" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YTV5</accession>
    <name>A0AA38YTV5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Movement protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_025120</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9676438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YTV5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051596">
        <property type="entry name" value="Caulimoviridae_Movement"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028919">
        <property type="entry name" value="Viral_movement"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47599">
        <property type="entry name" value="CELL-TO-CELL MOVEMENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47599:SF4">
        <property type="entry name" value="POLYPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01107">
        <property type="entry name" value="MP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9676438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="229" mass="25881" checksum="DEB45107016DFB32" modified="2024-01-24" version="1">MASLFRCKSSSSKAIVDSEEYRVSVEKNLHNWNIPKDKIYKTGFFKSNKLVRTEKQTTPLSTEDQIIRLLPQELLYRLQDHDCCLHVGLVQLAVGPLTRQGINGTSVVLCLQDNRHSKFEDSLLGKLQFSLCDGPIYFNCFPNFLVSLMDPRVFDCLNLSIKTNGYNMVRGSLPIALEFRVLYKIMRKTPAVPKALAENPGGRVLLEEKIGRSNAVFPASVKWSEVQDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YU55</accession>
    <name>A0AA38YU55_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">11-beta-hydroxysteroid dehydrogenase 1B-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_021883</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9676588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YU55"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072582">
        <property type="term" value="F:17-beta-hydroxysteroid dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008202">
        <property type="term" value="P:steroid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43391:SF89">
        <property type="entry name" value="11-BETA-HYDROXYSTEROID DEHYDROGENASE 1A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43391">
        <property type="entry name" value="RETINOL DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9676588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="346" mass="38293" checksum="CB68B3724274EDC8" modified="2024-01-24" version="1">MDIIHNFLNLVAPPFAFVTLFLLLPPFHFFKFFLSTFWSILSENVTGKVVIVTGASSGIGEHVAYEYAKRGACLVLAATRENRLQEVGGRARALGAPDVRVVPANVSKLEDCKRIVDEAANHFGRVDHLVNNAGIQSASTLEEATDITNFRPDVNFWGSVYTTRFAIPHLRNSHGKIIGMASSAAWLPMPRMSIYNASKAALISFYDTLRIELGSDIGGITVVTPGFIESEMTQGKFLKNEGKIEVDQDMSDAQVSIIPVGRAEACAKAIVKSACRGERYLTEPSWLRVTYLWKVFCPEVIEWCFRLFYLARPGESETLSKKILDISGARGFLYPTTIQTTDVKAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YVY2</accession>
    <name>A0AA38YVY2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GDSL esterase/lipase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_022424</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YVY2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051058">
        <property type="entry name" value="GDSL_Est/Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45648">
        <property type="entry name" value="GDSL LIPASE/ACYLHYDROLASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_4G14700)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45648:SF8">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041301666" description="GDSL esterase/lipase" evidence="5">
        <location>
            <begin position="19"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="352"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9677429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="385" mass="41626" checksum="D10E863BFD691863" modified="2024-01-24" version="1">MKLAVAWTIMVTTLLGVAMDGYDCKVVQFIFGDSLSDVGNNDRLSKSLAQASLPWYGIDFGNGLPNGRFCNGRTVADIIGDRTGLPRPPAFLDPSLTEDMILENGVNYASGGGGILNETGSLFIQRFSLYKQIELFQGTQELIKSKIGKEEAENFFQKSQYVVALGSNDFINNYLLPVYNDGWKYSDEGFINYLMETLKAQLTILHGLGARELMVFGLGPMGCIPLQRVLSTSGECQDKTNKLALSFNQAGSKMLKELSGNLPNASFQFGDAYDVVDDVITNPQKYGFNNSDSPCCSFGQIRPALTCVPASILCTDRSKYVFWDEYHPSDSANEIIATELIRKFGFLGRNGTNAPSPGPAVDAPSPGPAVDAPSPGLAVAPSPEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YY45</accession>
    <name>A0AA38YY45_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_020816</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YY45"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9678740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="111" mass="12825" checksum="131EC730CA1CDDCB" modified="2024-01-24" version="1">MPRARNISTTRPRRSTRSSPLSHGDGTKKRIAIYLATGSEYRNVSLIQNFKAPTPPIFRGGPNFLETENWLKEIKKILDVMAVHEEKRVSLASFMFRDEAENWWDMIKSTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YYD6</accession>
    <name>A0AA38YYD6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myb/SANT-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_021035</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YYD6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024752">
        <property type="entry name" value="Myb/SANT-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47072">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47072:SF4">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12776">
        <property type="entry name" value="Myb_DNA-bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb/SANT-like" evidence="2">
        <location>
            <begin position="17"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="175"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="194"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9678991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="300" mass="34235" checksum="83D52CF2D9E7F999" modified="2024-01-24" version="1">MATQLPSSQDNNNQAAKWDATRDAYLVEQLLYHQSIGRRPQSVWSKEAWRETRAAFNLKFSTSYNVKVFKNRLTKLKDQHNTIRELIGVNGFGWDDHYHMVTAENTVWDEYILKYPKAKHFRGKTFPLYNRLCIMFEGMSSEGRDAQSIGRPKVIDETENAQSQRHVAEVAYMDNDTVPSSDDETSSEQPIQNRTRMSPVSSTRRVRRRCESVGDKLISLLTDLLAGHAGKCHASQPLQTSAVSGEYSYSECLQELKRIEGLAMSEIVKGAHVLKDEQNRIAFMTFEGPARVAFLQSCFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YZM9</accession>
    <name>A0AA38YZM9_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_021544</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YZM9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9679650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="115" mass="13142" checksum="8EC69631ED3EBC75" modified="2024-01-24" version="1">MLESHWISSSVTFFILKEEAKARVVTDGRRLEKLEKVADAEECSPLTGLALRHFLNMRVSSLFLYNRFSLRPSLCFCRYTRNPSFLLSLFLSLSLSSTPFSKCLSSPCSNPPFLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YZS2</accession>
    <name>A0AA38YZS2_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_021548</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YZS2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9679654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="80" mass="8984" checksum="71E9D37C4AC5CE0C" modified="2024-01-24" version="1">MGSVAGFIRPMIKLIRLISRNSLRWHGMGTNMYPSSSLLGNIKGQMHALTNPLQLSPLLMVLLEWHRTLIVELQAGIRTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z0W6</accession>
    <name>A0AA38Z0W6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_019436</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase A1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z0W6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05476">
        <property type="entry name" value="pepsin_A_like_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000050">
        <property type="entry name" value="Aspartic proteinase CDR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034161">
        <property type="entry name" value="Pepsin-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051708">
        <property type="entry name" value="Plant_Aspart_Prot_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032799">
        <property type="entry name" value="TAXi_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032861">
        <property type="entry name" value="TAXi_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47967:SF39">
        <property type="entry name" value="ASPARTYL PROTEASE FAMILY PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47967">
        <property type="entry name" value="OS07G0603500 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14541">
        <property type="entry name" value="TAXi_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14543">
        <property type="entry name" value="TAXi_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Peptidase A1" evidence="8">
        <location>
            <begin position="1"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9680147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="326" mass="35156" checksum="B0469F41F517B727" modified="2024-01-24" version="1">MYMAFTTRAVTLCGHSVCLVCHATSRKTPFSCESQQCRLLDTVSCSQPQKLCDFSYGYGDGSLVQGVIATDTLTLNSNSGQPASIPKIVFGCGHNNSGTFNENEMGLFGTGGRPLSLTSQIMSTLGSGRKFSQCLVPFRTDPSITSKIIFGPEAEVSGSDVVSTPLITKDDPTYYFVTLDGISVGDKLFSFSSSSPMATKGNVFIDAGTPPTLLPRDFYNRLVQGVKEAIPMEPVQDPDLQPQLCYRSATLIDGPILTAHFDGADVQLKPLNTFISPKEGVYCFAMQPIDGDTGIFGNFVQMNFLIGFDLDGKKVSFKAVDCTKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z1S3</accession>
    <name>A0AA38Z1S3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NAD-dependent epimerase/dehydratase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_019954</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z1S3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08958">
        <property type="entry name" value="FR_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000085">
        <property type="entry name" value="Dihydroflavonol reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF563">
        <property type="entry name" value="CINNAMOYL-COA REDUCTASE 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="4">
        <location>
            <begin position="8"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9680793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="335" mass="37140" checksum="3A73AEDFBF9E4A50" modified="2024-01-24" version="1">MEGEKGKVCVTGGTGFIASWLIMKLLQHGYSVNATIRSHPQIKKDIGYLTNLPGASERLQIFKADLSEPQSFDAAIEGCIGVFHVAHPVDFEEREPQETVIRRSVEGTLGILKGCMKSKTVKRVVYTSSTAAVVYNNKDEDIMDESSWSDIDVINSIKPLGRSYVISKTRIERAALEFAEQHGLDLVSLIPSFVVGPFICPGFPGSVHFILAMILGNQHHYQYLKNTSMVHVDDVASAHIFLLEYPDAKGRYICSSNILTLYEMSELLSAKYPQLPIPAIDSLKEIQGFRIPGASSKKLLDAGFKYKYGVDEMFDEAIQCCKEKGFLNFSPDSIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z2D6</accession>
    <name>A0AA38Z2D6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_020137</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SINA (Seven in absentia) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9681091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z2D6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16571">
        <property type="entry name" value="RING-HC_SIAHs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000041">
        <property type="entry name" value="E3 ubiquitin-protein ligase SINAT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.210.10:FF:000027">
        <property type="entry name" value="Pectinesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.210.10">
        <property type="entry name" value="Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018121">
        <property type="entry name" value="7-in-absentia-prot_TRAF-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052088">
        <property type="entry name" value="E3_ubiquitin-ligase_SINA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049548">
        <property type="entry name" value="Sina-like_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008974">
        <property type="entry name" value="TRAF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013010">
        <property type="entry name" value="Znf_SIAH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10315">
        <property type="entry name" value="E3 UBIQUITIN PROTEIN LIGASE SIAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10315:SF80">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SINAT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21362">
        <property type="entry name" value="Sina_RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03145">
        <property type="entry name" value="Sina_TRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21361">
        <property type="entry name" value="Sina_ZnF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51081">
        <property type="entry name" value="ZF_SIAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="11">
        <location>
            <begin position="20"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="SIAH-type" evidence="12">
        <location>
            <begin position="73"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9681091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="270" mass="30256" checksum="FD3D0026AA1C390C" modified="2024-01-24" version="1">MGNRVADLHSLTKFQELLKCPVCLNFMQSPIYQCHNGHALCSSCKARVLNKCPSCRQRLSNIRCLALEKMAESLELHCEYEEFGCPEIIPYYTKLMHEDSCNFRPYSCPWSGCPCSAVGDIPLLVSHLTDYHKAVMFNSCNFKLGFLIGDLYKHQSYKCAVTIINCFGKHFCLHFDAFLIGSTPVYMAFLSLIGNQAETGNYSYSLEIGGNGRKLTFEGSPRSIRESERRSLESADSLIVLGSMALSLGGETRVPMLEFTGRIWKAQIPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z2G2</accession>
    <name>A0AA38Z2G2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BAG family molecular chaperone regulator 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_019916</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z2G2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009506">
        <property type="term" value="C:plasmodesma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040400">
        <property type="entry name" value="BAG5/6/7/8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33322">
        <property type="entry name" value="BAG DOMAIN CONTAINING PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33322:SF3">
        <property type="entry name" value="BAG FAMILY MOLECULAR CHAPERONE REGULATOR 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63491">
        <property type="entry name" value="BAG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9680744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="346" mass="39152" checksum="07E50921F06C9EB9" modified="2024-01-24" version="1">MFRRRMDIFEPQPLSPFTSSIETLDFSVFPEESLLSLPLPFFNPKHGLFDLRALPFDVFHTGFDRVVKASFEEGDRKHRWTAEIRSPGKDGFDCKYKWTAEIKGAKCSKLTPEIKGKHSDDIWTAEIKGPKSAAGECSRSAGKGSKKSVCASARCTAEIEHAGDHRAATSVRQASSGKKGGAIAEVKTKRKVLSPEDAALMTQMNFKNYLVRRSQSLQAIRELALAMNKLKELRALFNNFLYRRCIAHEAEQRQTFSERIIVLLLTVDAIEGIDLMVRAARRSMVNELEAMLDVTDPLLPRKMGSVRRRRKFHLPAEESIQKRVWQRLSKCSIKKINMGVTTLKPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z341</accession>
    <name>A0AA38Z341_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Disease resistance protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_020390</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Confers resistance to late blight (Phytophthora infestans) races carrying the avirulence gene Avr1. Resistance proteins guard the plant against pathogens that contain an appropriate avirulence protein via an indirect interaction with this avirulence protein. That triggers a defense system including the hypersensitive response, which restricts the pathogen growth.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9681495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z341"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009626">
        <property type="term" value="P:plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14798">
        <property type="entry name" value="RX-CC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038005">
        <property type="entry name" value="RX-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF1152">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0381">Hypersensitive response</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0611">Plant defense</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="9">
        <location>
            <begin position="6"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="8">
        <location>
            <begin position="180"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="10">
        <location>
            <begin position="427"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="11">
        <location>
            <begin position="544"/>
            <end position="891"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9681495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="933" mass="107133" checksum="A415BD103C6013F8" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z431</accession>
    <name>A0AA38Z431_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_017971</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9681857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z431"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9681857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="110" mass="12138" checksum="2583C8258D2D44AF" modified="2024-01-24" version="1">MMTTPWLPLHGPPRAERAGTLSYSQRPAKPPTPPPFPSPGAPMPVIRTPHMQISWRVPADTARFVEAMNEGPMEFRIFILLGYSNQKMDPLAYAEDAHLTLMQSNGGGVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z734</accession>
    <name>A0AA38Z734_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_019284</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054722">
        <property type="entry name" value="PolX-like_BBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039537">
        <property type="entry name" value="Retrotran_Ty1/copia-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057670">
        <property type="entry name" value="SH3_retrovirus"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648">
        <property type="entry name" value="TRANSPOSASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648:SF28">
        <property type="entry name" value="TRANSPOSON-ENCODED PROTEIN WITH RIBONUCLEASE H-LIKE AND RETROVIRUS ZINC FINGER-LIKE DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22936">
        <property type="entry name" value="Pol_BBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25597">
        <property type="entry name" value="SH3_retrovirus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="6">
        <location>
            <begin position="555"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="230"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="250"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9683643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1331" mass="151113" checksum="7A7B7E5B7E7594D8" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z7G4</accession>
    <name>A0AA38Z7G4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">PVL29_019283</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="24">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RLP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z7G4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009791">
        <property type="term" value="P:post-embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000453">
        <property type="entry name" value="Leucine-rich receptor-like protein kinase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000309">
        <property type="entry name" value="Leucine-rich repeat receptor protein kinase MSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000111">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001023">
        <property type="entry name" value="Os07g0541700 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001178">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051716">
        <property type="entry name" value="Plant_RL_S/T_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48053">
        <property type="entry name" value="LEUCINE RICH REPEAT FAMILY PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48053:SF126">
        <property type="entry name" value="MDIS1-INTERACTING RECEPTOR LIKE KINASE 2-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="16 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 22" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="403"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="469"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="497"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KAJ9683642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="649" mass="72345" checksum="1B281D753E12A08E" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZA01</accession>
    <name>A0AA38ZA01_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4283 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_017165</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZA01"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025558">
        <property type="entry name" value="DUF4283"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34427">
        <property type="entry name" value="DUF4283 DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34427:SF5">
        <property type="entry name" value="DUF4283 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14111">
        <property type="entry name" value="DUF4283"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4283" evidence="2">
        <location>
            <begin position="62"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="280"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="286"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="303"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9685022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="370" mass="40001" checksum="9F49866B1BEC9E3A" modified="2024-01-24" version="1">MVETSRSLGVVSGRKESQQVEATLLKPKLVKSFAEVVQTPRNKGRAVVRVEVRKEELSKNLNKLGHCLVGVWNPSSERGGDLKNWGCQMAKSWGLKGNLGMAKLERGKVLLEFDLVAEAKKALSHGRIWVGGSLLLLESWSPETGCLVEGEKRCEAWVRIVGLPVSLWDRAILRRVGKECGGFLAVDTQTEKLEELQWARILVKLNGEELPNVVEIWVEEVCYSLTLWWEVRSLIRFLLAGKCGKEVGAGEEVGDEGSVRAGKRMVEEDEGFRLEALFQSADETRGQTSGSGWPSVPSRNLDGSSGQPPGGLQLLGGPSKPGPGVESFIPTFGPVGPGPVLSASKEAGPVGLESQPLIEESGSNKGRGLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZAX4</accession>
    <name>A0AA38ZAX4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NB-ARC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_017618</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZAX4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766:SF40">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766">
        <property type="entry name" value="PLANT BROAD-SPECTRUM MILDEW RESISTANCE PROTEIN RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0611">Plant defense</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NB-ARC" evidence="2">
        <location>
            <begin position="54"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9685651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="196" mass="21828" checksum="2189483A29CB53CD" modified="2024-01-24" version="1">MESKVKEMIAKLEAIAQEKDGLGLKAGDGEKPSPRSASTSLVDESCVYGRDEIKEEMEHFDLKAWVCVSTEFLLLEVTKSILAAIGCRPTKDDSLDLLQRQPKESLVNKNFLLVLDDVWDVESFGWESWDSLRTPLIGAAQGSKILVTSRDESVAKTMRAVCTHPLGELNPPHCWSLFESMHFKIETPTHALSLNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZBA5</accession>
    <name>A0AA38ZBA5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_017772</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZBA5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13985">
        <property type="entry name" value="STKc_GAK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000349">
        <property type="entry name" value="probable serine/threonine-protein kinase DDB_G0280111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22967:SF57">
        <property type="entry name" value="AUXILIN, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22967">
        <property type="entry name" value="SERINE/THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="27"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="303"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="452"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="488"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="547"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="336"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="389"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="410"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="437"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="452"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="489"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="556"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="616"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="627"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="654"/>
            <end position="669"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9685836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="698" mass="77767" checksum="0EEF1796B31E672F" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZBG8</accession>
    <name>A0AA38ZBG8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Response regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_017418</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZBG8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009736">
        <property type="term" value="P:cytokinin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17581">
        <property type="entry name" value="REC_typeA_ARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045279">
        <property type="entry name" value="ARR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43874">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43874:SF106">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR ORR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="10"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="4">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9685379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="140" mass="15838" checksum="54EC421115983FDB" modified="2024-01-24" version="1">MGMAAESQFHVLAVDDSLIDRKWIEKLLKTSSFQVTVVDSVSKALEFLGVQEASQERKVNLIITDYSMPGMTGYDLLRKIKESTSLKDIPVVIMSSENIPSRINRCLEEGAEDFFLKPVQLSDVNKLRPHLLGWRTLVEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZE05</accession>
    <name>A0AA38ZE05_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycosyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_015962</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="5"/>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZE05"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035251">
        <property type="term" value="F:UDP-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000056">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000080">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050481">
        <property type="entry name" value="UDP-glycosyltransf_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48048">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48048:SF45">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9687285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="472" mass="52540" checksum="037E43CBB7A7AB7B" modified="2024-01-24" version="1">MKKVELVFVPSPAVGHLAATVEMAKLITDRDDRLSVTIFIMKLPFESEDSKTTESVASSIRFISLPRIEISSSSSTSPANFFTDVVKAYTPLAREAVHELMTRSGSVRLAGFVIDMFCTSMIDVANEFGVPSYLFFTSSAAFLGFVLHLQSLHDHHNLEITEFKDSDAELEVPSFVHSVPGKVFPSVVLDKEGDEIPVLLHHARRFRETKGIIVNTFVELESHAINSFSGDTSPPIYPIGPILNTEVESSEVQQQAIEIMNWLNDQPPSSVVFLCFGSMGSFNGEQVKEIAHGLEGSGCRFLWSLRQPPPKGKMEYPTEYGNKEEVLPEGFLDRTTKIGKVIGWAPQVAVLAHPAVGGFVSHCGWNSTLESLWYGVPTATWPMYAEQQLNAFQMVKDLELAVEIKIDYDKDKGYIVSSQDIGKGLRHLMDADSEVRKKRQKMQEKSRKAMMDGGSSYSYLGYFIEDMMTNVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZF14</accession>
    <name>A0AA38ZF14_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">GOLD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_016037</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the EMP24/GP25L family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZF14"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015720">
        <property type="entry name" value="Emp24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22811">
        <property type="entry name" value="TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01190">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041467819" description="GOLD domain-containing protein" evidence="9">
        <location>
            <begin position="29"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="10">
        <location>
            <begin position="38"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9687387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="216" mass="24079" checksum="4562F06F003C8689" modified="2024-01-24" version="1">MKMNGSGLVLRLLLLLCISDLLIPMSQAIWLSIPSSGTKCVSEEIQNNVVVLADYVVIPDDHTHSSPTISIKVTSPYGNNLHHKENVTHGQFAFTTSEAGNYLACFWVDGHNQGGGDVSVNLDWKTGIGAKDWESVARKEKIEGVELELRKLEGAVEAIHENLLYLKSREAEMRMVSEKTNARVAWYSIMSLGVCIAVSALQLWHLKRFFQKKKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZFN1</accession>
    <name>A0AA38ZFN1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Replication protein A subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PVL29_013953</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Component of the replication protein A complex (RPA) required for DNA recombination, repair and replication. The activity of RPA is mediated by single-stranded DNA binding and protein interactions. Probably involved in repair of double-strand DNA breaks (DSBs) induced by genotoxic stresses.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Heterotrimer of RPA1, RPA2 and RPA3 (canonical replication protein A complex).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the replication factor A protein 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9687979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZFN1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005662">
        <property type="term" value="C:DNA replication factor A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043047">
        <property type="term" value="F:single-stranded telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007140">
        <property type="term" value="P:male meiotic nuclear division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007004">
        <property type="term" value="P:telomere maintenance via telomerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04474">
        <property type="entry name" value="RPA1_DBD_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04475">
        <property type="entry name" value="RPA1_DBD_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04476">
        <property type="entry name" value="RPA1_DBD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04477">
        <property type="entry name" value="RPA1N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000041">
        <property type="entry name" value="Replication protein A subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000064">
        <property type="entry name" value="Replication protein A subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000090">
        <property type="entry name" value="Replication protein A subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000117">
        <property type="entry name" value="Replication protein A subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047192">
        <property type="entry name" value="Euk_RPA1_DBD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013955">
        <property type="entry name" value="Rep_factor-A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007199">
        <property type="entry name" value="Rep_factor-A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031657">
        <property type="entry name" value="REPA_OB_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004591">
        <property type="entry name" value="Rfa1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00617">
        <property type="entry name" value="rpa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23273">
        <property type="entry name" value="REPLICATION FACTOR A 1, RFA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23273:SF47">
        <property type="entry name" value="REPLICATION PROTEIN A 70 KDA DNA-BINDING SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04057">
        <property type="entry name" value="Rep-A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08646">
        <property type="entry name" value="Rep_fac-A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16900">
        <property type="entry name" value="REPA_OB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 12" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Replication factor-A protein 1 N-terminal" evidence="15">
        <location>
            <begin position="5"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="OB" evidence="14">
        <location>
            <begin position="193"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="Replication protein A OB" evidence="17">
        <location>
            <begin position="301"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="Replication factor A C-terminal" evidence="16">
        <location>
            <begin position="464"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="110"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU364130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF04057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF08646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF16900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ9687979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="623" mass="69879" checksum="89E2A9166D043BF4" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZGD8</accession>
    <name>A0AA38ZGD8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Alpha/beta-Hydrolases superfamily protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_014170</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZGD8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31934">
        <property type="entry name" value="ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31934:SF5">
        <property type="entry name" value="OS05G0557900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9688339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="542" mass="60974" checksum="56F6D01F0907B694" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZK61</accession>
    <name>A0AA38ZK61_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Cysteine-rich receptor-like protein kinase 29</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PVL29_012650</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZK61"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23509">
        <property type="entry name" value="Gnk2-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.430.20:FF:000002">
        <property type="entry name" value="Cysteine-rich receptor-like protein kinase 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000343">
        <property type="entry name" value="Cysteine-rich receptor-like protein kinase 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.430.20:FF:000003">
        <property type="entry name" value="Cysteine-rich RLK (RECEPTOR-like protein kinase) 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.430.20">
        <property type="entry name" value="Gnk2 domain, C-X8-C-X2-C motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002902">
        <property type="entry name" value="GNK2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038408">
        <property type="entry name" value="GNK2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002:SF1073">
        <property type="entry name" value="CYSTEINE-RICH RECEPTOR-LIKE PROTEIN KINASE 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002">
        <property type="entry name" value="RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD1-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01657">
        <property type="entry name" value="Stress-antifung"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51473">
        <property type="entry name" value="GNK2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="12 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 17" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041275930" description="Cysteine-rich receptor-like protein kinase 29" evidence="17">
        <location>
            <begin position="26"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="292"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="19">
        <location>
            <begin position="29"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="19">
        <location>
            <begin position="138"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="351"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="254"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="254"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAJ9690102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="22">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="670" mass="74267" checksum="323CAE60616A3944" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZKE7</accession>
    <name>A0AA38ZKE7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">PVL29_013065</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="23">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZKE7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048638">
        <property type="term" value="P:regulation of developmental growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000021">
        <property type="entry name" value="Putative LRR receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000015">
        <property type="entry name" value="Somatic embryogenesis receptor kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000016">
        <property type="entry name" value="Somatic embryogenesis receptor-like kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051824">
        <property type="entry name" value="LRR_Rcpt-Like_S/T_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48006">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN DDB_G0281931-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48006:SF90">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="14 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8 21" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041271295" description="non-specific serine/threonine protein kinase" evidence="21">
        <location>
            <begin position="20"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="232"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="294"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="322"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAJ9690721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="619" mass="68191" checksum="55B22AFB30B46725" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZMV7</accession>
    <name>A0AA38ZMV7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="17">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PVL29_011215</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13 17">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 17">Membrane</location>
            <topology evidence="1 17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="17"/>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZMV7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140326">
        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02073">
        <property type="entry name" value="P-type_ATPase_APLT_Dnf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000023">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000050">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000014">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF150">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 15 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="8 16 17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 15 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 17" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="85"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="109"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="305"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="357"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="946"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="975"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1025"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1063"/>
            <end position="1082"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1094"/>
            <end position="1112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1132"/>
            <end position="1151"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="18">
        <location>
            <begin position="43"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="19">
        <location>
            <begin position="911"/>
            <end position="1161"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="14">
        <location>
            <position position="425"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="425"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="425"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="426"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="427"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="427"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="573"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="620"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="643"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="677"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="758"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="759"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="760"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="859"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="865"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="885"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="888"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="889"/>
        </location>
        <ligand>
            <name>ATP</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="889"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU362033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF16209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF16212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAJ9692013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1229" mass="139037" checksum="B0873CC41505ADAF" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZPD2</accession>
    <name>A0AA38ZPD2_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_011180</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9691948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZPD2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36721:SF1">
        <property type="entry name" value="OS04G0446401 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36721">
        <property type="entry name" value="PROLINE-RICH FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="150"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9691948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="189" mass="20379" checksum="1D7D8B8EA8303611" modified="2024-01-24" version="1">MEQPFSTLLFVILGFGFCIYAVLVDAQSKVESQPNTTSPPPPIYRPPPSPPSPAPPPLPSPPPRPPAVPPPPTPLSPPPPPQSPSTPPPSSPPPPQRPRPRPRPNTTNKNSTDHSKNAATDPTSNVNPNQMNNDSAEHHHGQKINLGKTIGLLFVGIAGILQIFVVGFLTFKRRQLLKLKDRYETCSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZT71</accession>
    <name>A0AA38ZT71_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Proline-rich protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_010175</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZT71"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010227">
        <property type="term" value="P:floral organ abscission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039639">
        <property type="entry name" value="IDA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33599:SF13">
        <property type="entry name" value="FORMIN-LIKE PROTEIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33599">
        <property type="entry name" value="PROTEIN IDA-LIKE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041318886" description="Proline-rich protein" evidence="5">
        <location>
            <begin position="28"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="42"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9694564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="286" mass="30283" checksum="4D495157A3C019EE" modified="2024-01-24" version="1">MEQRCGLIGRRLMVLLVLGCVLCLANAQRHAMNFVMLPKGDPIPPSGPSHKGSETPPPPHSFARGPTLRFGMLPKGVPIPPSGPSHGSSDPPPPPQPFARVSTFRFGMLPKGDPIPPSGPSHKGSDTPPSPHPFTRTPTLRFRMLPKGVPIPPSGPSHGSSDPPPPPQPFARVSTFRFGMLPKGDPIPPSGPSHKGSDTPPSPHPFTRTPTLRFRMLPKGVPIPPSGPSHGSSDAPPPPQPFARASVFRFGMLPKGDPIPPSGPSHKGSDTPPSPHPFTRTPTLRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZU07</accession>
    <name>A0AA38ZU07_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Guanylate kinase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_010041</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IPP transferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZU07"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009824">
        <property type="term" value="F:AMP dimethylallyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052381">
        <property type="term" value="F:tRNA dimethylallyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009691">
        <property type="term" value="P:cytokinin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.890">
        <property type="entry name" value="Crystal structure of tRNA isopentenylpyrophosphate transferase (bh2366) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039657">
        <property type="entry name" value="Dimethylallyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11088:SF86">
        <property type="entry name" value="ADENYLATE ISOPENTENYLTRANSFERASE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11088">
        <property type="entry name" value="TRNA DIMETHYLALLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01715">
        <property type="entry name" value="IPPT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0203">Cytokinin biosynthesis</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Guanylate kinase-like" evidence="7">
        <location>
            <begin position="12"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="188"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="188"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9694349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="265" mass="29601" checksum="D27699741A00FED2" modified="2024-01-24" version="1">MDSTVRRNRPKNKVVVIMGATGTGKSKLSSDLATRFPAEIINSDKIQIYSGLDITTNKIQMHERQGVPHHLLGDFDSFHAEITPSQFRSVAAAAISDISSRRKLPVLAGGSNSFIHALLVDRFDSESDPFNGSDSVSTELRYHCCFLWVDVSFAVLSDYLSKRVDEMLDSGMLEELAKFYDPVADESRPKTLVEKSDRSPRVRQAFPKVPSRRPRNNREGQDNTCHLPKKQIEKILRMRGGNLCGIKPVDHECVNKGSGPTSEDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZWE1</accession>
    <name>A0AA38ZWE1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dirigent protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_010865</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant dirigent protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZWE1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004265">
        <property type="entry name" value="Dirigent"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21495">
        <property type="entry name" value="NUCLEOPORIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03018">
        <property type="entry name" value="Dirigent"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0052">Apoplast</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0964">Secreted</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9695593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="98" mass="10672" checksum="D6DD244655D7CAB5" modified="2024-01-24" version="1">MVRQIDAEIFSTIVAAAASGSSEESHRFSRKFSPISIGLKEEKLSHLHFYFHDIVSGQQPTAVRVAEAAMTNKSGTVFMMDDLLTEGPEPSSKMVGKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZWI9</accession>
    <name>A0AA38ZWI9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Histone H2A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_008528</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="8">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the histone H2A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZWI9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00074">
        <property type="entry name" value="HFD_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000013">
        <property type="entry name" value="Core histone macro-H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002119">
        <property type="entry name" value="Histone_H2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032454">
        <property type="entry name" value="Histone_H2A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032458">
        <property type="entry name" value="Histone_H2A_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23430">
        <property type="entry name" value="HISTONE H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16211">
        <property type="entry name" value="Histone_H2A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00620">
        <property type="entry name" value="HISTONEH2A"/>
    </dbReference>
    <dbReference type="SMART" id="SM00414">
        <property type="entry name" value="H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00046">
        <property type="entry name" value="HISTONE_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 8" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="10">
        <location>
            <begin position="18"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="Histone H2A C-terminal" evidence="11">
        <location>
            <begin position="97"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9696349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="137" mass="14888" checksum="C22A7D6E05F93E04" modified="2024-01-24" version="1">MSSSSSTKAGKGRSRASASVTCSRRAGLQFPVSRISRLIKDGNYAECVSRTAPVYLSAVLEYLSAEVLELAGNVAKDNQKARILPRHIVHAMRNDIELCRLLENVTIADGGVLPNIDEDFLPEEEKGKGNRSATEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZWV0</accession>
    <name>A0AA38ZWV0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nuclear envelope-associated protein 2-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_008812</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZWV0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049932">
        <property type="entry name" value="NEAP1-4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48145">
        <property type="entry name" value="NUCLEAR ENVELOPE-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48145:SF5">
        <property type="entry name" value="NUCLEAR ENVELOPE-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="132"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="240"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9696786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="335" mass="38867" checksum="D5472141996A1E15" modified="2024-01-24" version="1">MPESKKPELDPLLKDLSEKKQIFRRNVVSLAAELKDARARLTLQQESCTRETLTRQVVEKRTRSMEDEICQLQKTLEERNCQLEAFGSDANRFLKELDDIRLQLSGTQATADISARSAQSAQLQCLALAKELNVKNSALKEHEDRANRLAEQLNMLHIDLEAREASQKQLKDEVLRIEKDIMQAVSRAEKGKNCELMKILDEVSPKNFEKIGGFLTMKDEEIGRLRDDIRVLSAHWERKTKELESQLEKNRRADQDLKKRVLKLEFCLQEARSQTRKLQRIEERRDKALKEIRGQLATKQRETADVAEKQNFWESSTFKMVVSMSMLVLVVFAKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZXR2</accession>
    <name>A0AA38ZXR2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">CRAL-TRIO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_009171</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the SFH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZXR2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.525.10:FF:000011">
        <property type="entry name" value="SEC14 cytosolic factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.20">
        <property type="entry name" value="N-terminal domain of phosphatidylinositol transfer protein sec14p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051026">
        <property type="entry name" value="PI/PC_transfer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45657">
        <property type="entry name" value="CRAL-TRIO DOMAIN-CONTAINING PROTEIN YKL091C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45657:SF43">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL_PHOSPHATIDYLCHOLINE TRANSFER PROTEIN SFH9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03765">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00180">
        <property type="entry name" value="CRETINALDHBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM01100">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041426006" description="CRAL-TRIO domain-containing protein" evidence="9">
        <location>
            <begin position="24"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="459"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="domain" description="CRAL-TRIO" evidence="10">
        <location>
            <begin position="156"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9697260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="615" mass="70042" checksum="22BB0D18E7003411" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZY28</accession>
    <name>A0AA38ZY28_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UV-B-induced protein At3g17800, chloroplastic-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_009307</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZY28"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038925">
        <property type="entry name" value="At3g17800-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008479">
        <property type="entry name" value="DUF760"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31808">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31808:SF9">
        <property type="entry name" value="F21O3.2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05542">
        <property type="entry name" value="DUF760"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9697428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="366" mass="41585" checksum="25AB10A3A4428E67" modified="2024-01-24" version="1">MDYCLSRSTVLFPHLQITSSYHDHDCFPRSTTLASSKSRSSSLVVVASAEAISSLNTPLEPRSLPGKFLSGVLQNQRQLFHVAVAEQLRDLADDRDAALARMNLSYGSSSSSESCLHRRIAELKERECQIAVEDVMYLLIFYKFSEIRVHLVPRLSRCIYNGRLEIWPARDWALECIHNFEVLEMIKEHLTTVLGWKPKSNVTENWATTQIRRFQLGQIYAASILYGYFLKSASLRHHLEMSLVHTHHDLPSSNVSGFWSYGLKDLFLGPNGRGKRGKKLRCYVMGFDPDTLQRCAKLKSKEAVNLVEKHSCALFGDEKTGLLETDDVISTSFSSMKRLVLEAVAFGSFLWDTEEYVSSVYNLKEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZY86</accession>
    <name>A0AA38ZY86_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase Ty1/copia-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_009371</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZY86"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase Ty1/copia-type" evidence="1">
        <location>
            <begin position="3"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9697518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="110" mass="12498" checksum="F5410DDE4744702F" modified="2024-01-24" version="1">MLRSEADHSVFYHHNSSNQCIYLVVYVDDIVITGSDQEGIQRLKQYLFDHFQTKDLGKLKYFLGLEIAQSSSGVVMSQRKYALDILEETGMLECKPVDTPMDPNVKLVPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZYE6</accession>
    <name>A0AA38ZYE6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Glyoxylate/succinic semialdehyde reductase 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_009445</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the NADPH-dependent reduction of glyoxylate to glycolate as well as succinic semialdehyde (SSA) to gamma-hydroxybutyrate in vitro. May function in redox homeostasis and play a role in oxidative stress tolerance by detoxifying glyoxylate and SSA generated in glycolate metabolism and GABA metabolism, respectively.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>4-hydroxybutanoate + NADP(+) = succinate semialdehyde + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:26381"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16724"/>
            <dbReference type="ChEBI" id="CHEBI:57706"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.n11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>glycolate + NADP(+) = glyoxylate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10992"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29805"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.79"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HIBADH-related family. NP60 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZYE6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030267">
        <property type="term" value="F:glyoxylate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.10:FF:000016">
        <property type="entry name" value="Glyoxylate/succinic semialdehyde reductase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000058">
        <property type="entry name" value="Putative oxidoreductase GLYR1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002204">
        <property type="entry name" value="3-OH-isobutyrate_DH-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006115">
        <property type="entry name" value="6PGDH_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029154">
        <property type="entry name" value="HIBADH-like_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015815">
        <property type="entry name" value="HIBADH-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051265">
        <property type="entry name" value="HIBADH-related_NP60_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43580:SF9">
        <property type="entry name" value="GLYOXYLATE_SUCCINIC SEMIALDEHYDE REDUCTASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43580">
        <property type="entry name" value="OXIDOREDUCTASE GLYR1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14833">
        <property type="entry name" value="NAD_binding_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03446">
        <property type="entry name" value="NAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000103">
        <property type="entry name" value="HIBADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00895">
        <property type="entry name" value="3_HYDROXYISOBUT_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="6-phosphogluconate dehydrogenase NADP-binding" evidence="10">
        <location>
            <begin position="2"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="3-hydroxyisobutyrate dehydrogenase-like NAD-binding" evidence="11">
        <location>
            <begin position="164"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00052582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00052769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00056683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000103-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF14833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9697620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="291" mass="30875" checksum="9061C9EEB1670EE0" modified="2024-01-24" version="1">MEVGFLGLGIMGKAMSINLIRSGFKLTVWNRTLSKCDELVELGASIGETPAAVVKKCKYTIAMLSDPSVALSVVFDKDGVLEQICHGKGYIDMSTVDADTSSKISEAITSKGGSFLEAPVSGSKKPAEDGQLVILAAGEKALYDEAIPAFDIMGKKSFFLGQVGNGAKMKLVVNMIMGSMMNAFSEGLVLADRSGLNPHTLLDVLDLGGIANPMFRLKGPTMIQNSYSPAFPLKHQQKDMRLALALGDENAVSMPVAAAANEAFKKARSLGLGDLDFSAVYETVKTLEHSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZYG4</accession>
    <name>A0AA38ZYG4_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_009370</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CAF1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZYG4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051181">
        <property type="entry name" value="CAF1_poly(A)_ribonucleases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006941">
        <property type="entry name" value="RNase_CAF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15092">
        <property type="entry name" value="POLY A -SPECIFIC RIBONUCLEASE/TARGET OF EGR1, MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15092:SF42">
        <property type="entry name" value="POLY(A)-SPECIFIC RIBONUCLEASE PARN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04857">
        <property type="entry name" value="CAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="643"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="643"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="656"/>
            <end position="669"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9697517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="676" mass="75380" checksum="90B1AB584CA89804" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZZZ7</accession>
    <name>A0AA38ZZZ7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Lipoxygenase</fullName>
            <ecNumber evidence="18">1.13.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PVL29_007147</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Plant lipoxygenase may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 17">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="18">Lipid metabolism; oxylipin biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the lipoxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.-" evidence="18"/>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9697887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZZZ7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016165">
        <property type="term" value="F:linoleate 13S-lipoxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034440">
        <property type="term" value="P:lipid oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031408">
        <property type="term" value="P:oxylipin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.245.10:FF:000002">
        <property type="entry name" value="Lipoxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.60:FF:000005">
        <property type="entry name" value="Lipoxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.375.10">
        <property type="entry name" value="Lipoxygenase-1, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.372.10">
        <property type="entry name" value="Lipoxygenase-1, Domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.245.10">
        <property type="entry name" value="Lipoxygenase-1, Domain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.60.20">
        <property type="entry name" value="PLAT/LH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000907">
        <property type="entry name" value="LipOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013819">
        <property type="entry name" value="LipOase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036226">
        <property type="entry name" value="LipOase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020834">
        <property type="entry name" value="LipOase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020833">
        <property type="entry name" value="LipOase_Fe_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001246">
        <property type="entry name" value="LipOase_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027433">
        <property type="entry name" value="Lipoxygenase_dom_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001024">
        <property type="entry name" value="PLAT/LH2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036392">
        <property type="entry name" value="PLAT/LH2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11771">
        <property type="entry name" value="LIPOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00305">
        <property type="entry name" value="Lipoxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01477">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00087">
        <property type="entry name" value="LIPOXYGENASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00468">
        <property type="entry name" value="PLTLPOXGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00308">
        <property type="entry name" value="LH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49723">
        <property type="entry name" value="Lipase/lipooxygenase domain (PLAT/LH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48484">
        <property type="entry name" value="Lipoxigenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00711">
        <property type="entry name" value="LIPOXYGENASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00081">
        <property type="entry name" value="LIPOXYGENASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51393">
        <property type="entry name" value="LIPOXYGENASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50095">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="11 17" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="15 18" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="13 17" id="KW-0408">Iron</keyword>
    <keyword evidence="4 18" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="14" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 17" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8 18" id="KW-0925">Oxylipin biosynthesis</keyword>
    <keyword evidence="6" id="KW-0934">Plastid</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="PLAT" evidence="19">
        <location>
            <begin position="82"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoxygenase" evidence="20">
        <location>
            <begin position="216"/>
            <end position="892"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU003975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAJ9697887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="892" mass="101881" checksum="D36CD0DE8783C045" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A0S0</accession>
    <name>A0AA39A0S0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Pseudouridine synthase I TruA alpha/beta domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_007763</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a uridine in tRNA = a pseudouridine in tRNA</text>
            <dbReference type="Rhea" id="RHEA:54572"/>
            <dbReference type="Rhea" id="RHEA-COMP:13339"/>
            <dbReference type="Rhea" id="RHEA-COMP:13934"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the tRNA pseudouridine synthase TruA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A0S0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990481">
        <property type="term" value="P:mRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02568">
        <property type="entry name" value="PseudoU_synth_PUS1_PUS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.580:FF:000002">
        <property type="entry name" value="tRNA pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.660:FF:000011">
        <property type="entry name" value="tRNA pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.660">
        <property type="entry name" value="Pseudouridine synthase I, catalytic domain, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.580">
        <property type="entry name" value="Pseudouridine synthase I, catalytic domain, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001406">
        <property type="entry name" value="PsdUridine_synth_TruA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020097">
        <property type="entry name" value="PsdUridine_synth_TruA_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020095">
        <property type="entry name" value="PsdUridine_synth_TruA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041708">
        <property type="entry name" value="PUS1/PUS2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020094">
        <property type="entry name" value="TruA/RsuA/RluB/E/F_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142:SF9">
        <property type="entry name" value="TRNA PSEUDOURIDINE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01416">
        <property type="entry name" value="PseudoU_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Pseudouridine synthase I TruA alpha/beta" evidence="8">
        <location>
            <begin position="342"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="243"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="293"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="125"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00036943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR641708-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR641708-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9698841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="516" mass="58284" checksum="75860912C1EA3E52" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A3V8</accession>
    <name>A0AA39A3V8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_005939</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A3V8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF85">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041272210" description="Reverse transcriptase domain-containing protein" evidence="1">
        <location>
            <begin position="25"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9700381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="246" mass="28174" checksum="ADFB3DEEA99BE3DF" modified="2024-01-24" version="1">MGFGEKWTGWISLCISTATYSVLANGTPASFFNSSRGLRQGDPLSPYLFVIRMEALRRLIFRAVREGYLSGCKIKRRSGDEVLVSHLLFANDTLVFREASQDQMAYLSWLLMWFEAISGLRINLDKSEISPVGRVENLEGLALEFGCKVGRLPTSYLGLPLGAYHKFVVIWDGVEEKFRKRLAMWKRQFISKGERITLIRSTLSSMPIYLMSLLHIPRVVSLRLEKIKRDFLWKGGVLERKPHLVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A754</accession>
    <name>A0AA39A754_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_004047</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A754"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010020">
        <property type="term" value="P:chloroplast fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003425">
        <property type="entry name" value="CCB3/YggT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33219:SF14">
        <property type="entry name" value="PROTEIN COFACTOR ASSEMBLY OF COMPLEX C SUBUNIT B CCB3, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33219">
        <property type="entry name" value="YLMG HOMOLOG PROTEIN 2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02325">
        <property type="entry name" value="CCB3_YggT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9702121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="229" mass="25239" checksum="96542BE6E706F98D" modified="2024-01-24" version="1">MKGLYAISNRFLVPLIGMNKIECCFQIHCRKQRNLNPLSNQNFAAILPGDSVAGIVVANGILNFLNIYNTLLIVRLVLTWFPNSPPAIVSPLRKVLMTSPSRTVYMVQKPTPPECQSPFLRPLPSESHGVCYNLWFCEQPNTLCDPYLNIFRGIIPPLGGTLDLSPILAFLVLNAFTSTAAALPAELPTAGVPQQIPSSHTRLFDLTTTQKKWMRRLCGNGSKSSSSVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A8Q9</accession>
    <name>A0AA39A8Q9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Legumain prodomain domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_003927</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9701924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A8Q9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006624">
        <property type="term" value="P:vacuolar protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21115">
        <property type="entry name" value="legumain_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.130:FF:000001">
        <property type="entry name" value="Vacuolar-processing enzyme beta-isozyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1460:FF:000005">
        <property type="entry name" value="Vacuolar-processing enzyme beta-isozyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043577">
        <property type="entry name" value="AE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048501">
        <property type="entry name" value="Legum_prodom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046427">
        <property type="entry name" value="Legumain_prodom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001096">
        <property type="entry name" value="Peptidase_C13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12000">
        <property type="entry name" value="HEMOGLOBINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12000:SF50">
        <property type="entry name" value="VACUOLAR-PROCESSING ENZYME GAMMA-ISOZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20985">
        <property type="entry name" value="Legum_prodom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01650">
        <property type="entry name" value="Peptidase_C13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500139">
        <property type="entry name" value="AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019663">
        <property type="entry name" value="Legumain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00776">
        <property type="entry name" value="HEMOGLOBNASE"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0788">Thiol protease</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041342331" description="Legumain prodomain domain-containing protein" evidence="9">
        <location>
            <begin position="24"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="domain" description="Legumain prodomain" evidence="10">
        <location>
            <begin position="383"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="179"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR019663-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF20985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9701924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="496" mass="54241" checksum="377FE1D5B60986EA" modified="2024-01-24" version="1">MTVFPAAIAAVAALLALATLVAGGRHFAGDNGLLLPSEASRFFRPGGAADDDTGGESAGTRWAVLIAGSNGYWNYRHQADICHAYQLLKKGGLKDENIIVLMYDDISFNEENPRPGIIINSPHGEDVYEGVPKDYTGEDVTVDNFFAVILGNKTALSGGSGKVLDSGPNDHIFIYYSDHGGPGVLGMPTSPYLYANDLIEVLKKKHASGTYKSLVFYLEACESGSIFEGLLPEGLNIYATTAANAEESSWGTYCPGEDPSPPPEYETCLGDLYSVAWMEDSDVHNLRTETLRQQYELVKKRTANDNSVYGSHVMQYGDLNLNKEDLVLYMGTNPANDNYTYVDDNSLRLPSKAVNQRDADLVHFWDKFRKAPEGSPRKAEAQKQFLEAMSHRTHIDHAIKLVGRLLFGIKKGSEVLKTVRPAGQPLVDDWHCLKTLVRTFEAHCGSLSQYGMKHMRSIANICNAGIEKEQMAEASAQACVTIPPGPWSSLDKGFSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AC21</accession>
    <name>A0AA39AC21_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UspA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_003024</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AC21"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23659">
        <property type="entry name" value="USP_At3g01520-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47583">
        <property type="entry name" value="ADENINE NUCLEOTIDE ALPHA HYDROLASES-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47583:SF1">
        <property type="entry name" value="ADENINE NUCLEOTIDE ALPHA HYDROLASES-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="1">
        <location>
            <begin position="41"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9704777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="173" mass="18928" checksum="98BF1450468141A4" modified="2024-01-24" version="1">MEPVKEDVEEYGWREVKLPSLIPVVPPPELGRETGERRRGRDIIVAVDHGPNSKLAFDWTLIHLCRLADTIHLVNAVSSVSNEVVYESSQTLMEKLAIEAYEVAMVRTVARIVEGDAGKAICKEAERILPVAVVMGTRGRSLLQSVLQGSVSEYVFHNCTAAPVIIVPGIGEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ACQ3</accession>
    <name>A0AA39ACQ3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_002957</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Sodium/anion cotransporter (TC 2.A.1.14) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ACQ3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17380">
        <property type="entry name" value="MFS_SLC17A9_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000199">
        <property type="entry name" value="Probable anion transporter 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000220">
        <property type="entry name" value="Probable anion transporter 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050382">
        <property type="entry name" value="MFS_Na/Anion_cotransporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044777">
        <property type="entry name" value="SLC17A9-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662:SF282">
        <property type="entry name" value="ANION TRANSPORTER 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="78"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="101"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="235"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="282"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="316"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="340"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="371"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="412"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="12"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9704652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="442" mass="48153" checksum="0CF334F2817EBBBF" modified="2024-01-24" version="1">MGRLKFPKRYLIVILTFISTCVCYVERVGFSIAYTVAADAAGVNQSSKGTILSTFYYGYACSQVPGGWAAQKLGGRRVLLFSFVLWSLTCALFPLDPNHVMLMVMARLLVGVAQGFIFPSIHTVLAQWVPPHERSRSVSLTTSGMYLGAAAGMLVLPSLVKYRGPQSVFFAEATLGAMWSLLWLKYASDPPRAEYPKATASGFGESLLPMRGSQKVKVENGGNSVKTSKIPWKRIFISLPVWAIVVNNFTFHYALYVLMNWLPTYFELGLRLSLQEMGSSKMLPYLNMFIFSNIGGVVADHLITKRIMSVTKTRKFLNTIGFIVASLALMALPTFRTSGGAVFCSSLALGFLALGRAGFAVNHMDVAPRYAGIVMGVSNTAGTLAGIVGVDLTGKLLEAAQTANSDLSGAESWRLVFFIPGLLCIFSSFVFLVFSTGERIFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AF36</accession>
    <name>A0AA39AF36_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Aluminum-activated malate transporter 9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_001572</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the aromatic acid exporter (TC 2.A.85) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AF36"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015743">
        <property type="term" value="P:malate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034220">
        <property type="term" value="P:monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020966">
        <property type="entry name" value="ALMT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31086">
        <property type="entry name" value="ALUMINUM-ACTIVATED MALATE TRANSPORTER 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11744">
        <property type="entry name" value="ALMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0407">Ion channel</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="113"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="136"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="167"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="197"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="430"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="434"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9706069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="543" mass="60438" checksum="0B5C58925D348949" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AG39</accession>
    <name>A0AA39AG39_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Hydrophobic seed protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_002034</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant LTP family. PEARLI1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AG39"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01958">
        <property type="entry name" value="HPS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.110.10">
        <property type="entry name" value="Plant lipid-transfer and hydrophobic proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036312">
        <property type="entry name" value="Bifun_inhib/LTP/seed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027923">
        <property type="entry name" value="Hydrophob_seed_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051636">
        <property type="entry name" value="Plant_LTP/defense-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31731">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14547">
        <property type="entry name" value="Hydrophob_seed"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47699">
        <property type="entry name" value="Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041218989" description="Hydrophobic seed protein domain-containing protein" evidence="3">
        <location>
            <begin position="26"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="Hydrophobic seed protein" evidence="4">
        <location>
            <begin position="145"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="30"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="93"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="54"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="104"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9706876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="230" mass="23037" checksum="D543835F80BFC688" modified="2024-01-24" version="1">MGSKGTAMFFNFMIFMLMALPPMQACGYCSQPQPPSRYPSPHYRHPNPPTIPHPHPHPSPKKHPPVIVPPIIVTPPVITPPVVNPPVTIPPPSSTYPPYTGNPPSGGGGGGGGGGRGGGGGGGGGGGGGGGGLPGLNPPPSTSPTCPVNAIKLGACVDVLGGLVHIGLGNPVENVCCPVLGGLLELEAAVCLCTAIRLKLLNLNIFIPIALQALITCGKTPPPGFICPPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AIF5</accession>
    <name>A0AA39AIF5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_002107</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Nudix hydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AIF5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047631">
        <property type="term" value="F:ADP-ribose diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035529">
        <property type="term" value="F:NADH pyrophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04670">
        <property type="entry name" value="NUDIX_ASFGF2_Nudt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000016">
        <property type="entry name" value="nudix hydrolase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000015">
        <property type="entry name" value="Nudix hydrolase 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003293">
        <property type="entry name" value="Nudix_hydrolase6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040618">
        <property type="entry name" value="Pre-Nudix"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13994:SF13">
        <property type="entry name" value="FI03680P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13994">
        <property type="entry name" value="NUDIX HYDROLASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18290">
        <property type="entry name" value="Nudix_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01356">
        <property type="entry name" value="GFGPROTEIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="4">
        <location>
            <begin position="185"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9706978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="374" mass="42229" checksum="07EC76DB4FCF8137" modified="2024-01-24" version="1">MELKLFDSKSVSVSDVDLVGRPCSSMLSNSRGGRIFPWFCSYRGVSPKVSYSNTSSKAIQSLGQEKPGVETFSYRFNGTNGTRPTLFSREMRVLDAIDDEYGGVIVDPQRLPVNPSSFAMVLHSSLSHWKMQGKKGVWLKLPVERSELVPIAVKEGFQYHHAERGYVMLTYWIPEGPCMLPSNASHHVGVGGFVINDKNEVLVVQEKHYAPALVGLWKIPTGFILEAEEISTGVVREIKEETGVRTFVLGKYFLSRILFHLIFRIEWCRRHAHNVAFEKSDLFFVCMLRPLSTQIAVDDIEIQAAKWMPLDEFVEQPLIQGDCMFKKIIDICIARLGKRYCGLSAQQLVSKFDGKLSSLYYNVTEAQDLNCQGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AJM5</accession>
    <name>A0AA39AJM5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein DETOXIFICATION</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Multidrug and toxic compound extrusion protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_000700</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AJM5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13132">
        <property type="entry name" value="MATE_eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045069">
        <property type="entry name" value="MATE_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="142"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="175"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="206"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="279"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="317"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="355"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="385"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="418"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="446"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="8"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9708812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="500" mass="54509" checksum="D89D21C24DD4220D" modified="2024-01-24" version="1">MENDEQPLLSTTHDQEQTQNPRPAKTGFFSFLAANYDDDMDPINGVGDFFREFRVESKKLWHIAGPAIFTYLCRYSLGAVTQVFVGHIGALQLAAFAVENSVISMFSLGTMLGMGSALETLCGQAFGAGQLDMLGVYMQRSWIILVTTALPLSLIYIFAEQILKLIGETEEISKAAGVFALWMLPQLFSYALNFPISKFLQSQRKMLVLSLTAGVTLVLHAFFSWLLIMKLGWGLVGAAVVLNASSWLPVVANFSYILSGSCGPAWSGFSSKAFHNLWAFVKLSLASAVMLCLEVWYFMVLVLFAGYLKNAEVSVDALSICMTILGWGVMLSIGFNAAVSVRVSNELGASHPRTAKFSVAVAAITSFLISVYPDLFSSNAEIKKLVYSLTPLLAVCIVINNIQPVLSGVAVGAGWQAFIAYVNIGCYYVIGVPMGLLMGFKLDLGVKGIWCGMLSGTVIQTCILFGMIYRTNWNTEASMAEERIRKWGGAVTAKENHSEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AQ39</accession>
    <name>A0AA39AQ39_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Remorin C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_001279</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the remorin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AQ39"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005516">
        <property type="entry name" value="Remorin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31471:SF52">
        <property type="entry name" value="F12A21.28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31471">
        <property type="entry name" value="OS02G0116800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03763">
        <property type="entry name" value="Remorin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Remorin C-terminal" evidence="4">
        <location>
            <begin position="246"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="157"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="279"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="79"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="98"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="161"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="183"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9709723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="359" mass="40155" checksum="48E55FF7351C3A2E" modified="2024-01-24" version="1">MRSIEDKGCCNYGPTKDISSSSAISFEFPKGNGTNRTTHHRTALGKPTPSKWDDAQKWLVGLSRGGEKNQSKTKPRNSNADDRRLIAPVPQKEKDYSSYEDEGEEEANGCPGPASSNQYQVETKKVDCDESFWQINKASEDSSSAVRSICVRDMGTEMTPMASQEPSRTATPIRATTPVARSPISSGSSTPGRCYQTGLSSTESRGEAAPVGRRHGRFISDGEESNACKMPENNNSEHARKPNPLDARATAWDEAERAKYLARYKREEVKIQAWENHEKRKAEMEMRRMEVKADKLKARAQEKLANKIAATRRIAEEKRANAEAKLNEKAARTSERADYIRRTGHLPSSFYFKLPSLCW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ARK3</accession>
    <name>A0AA39ARK3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CRAL-TRIO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_001478</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9710023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ARK3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46277:SF3">
        <property type="entry name" value="BINDING PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46277">
        <property type="entry name" value="OS03G0850700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00180">
        <property type="entry name" value="CRETINALDHBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM01100">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="1">
        <location>
            <begin position="82"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9710023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="248" mass="28279" checksum="868DD3FA0EEE146A" modified="2024-01-24" version="1">MEVEVEANGLQDSLESNEVEKRKVGLMRARVESEDPSSKDLDDFMIRRFLRARDLDIDKASALLLKYLGWRRAFIPNGSISTSEIPNELAQNKLFMQGQDKKGRPIIVVFGGRHKPYKGHLEEFKRFVVYCLERICASMPGGEEKFIAIADIEGWGYTNSDIRAYLAALSILQDCYPERLGKLFLVHVPYIFMTAWKVVYPFIDSKTKKKIIFVENKNIRSALLGDIDESQLPDVYGGKLPLVPIQDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D4K5</accession>
    <name>A0AA39D4K5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Double-strand break repair protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">PVL29_025456</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="22">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Core component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The MRN complex is involved in the repair of DNA double-strand breaks (DSBs) via homologous recombination (HR), an error-free mechanism which primarily occurs during S and G2 phases. The complex (1) mediates the end resection of damaged DNA, which generates proper single-stranded DNA, a key initial steps in HR, and is (2) required for the recruitment of other repair factors and efficient activation of ATM and ATR upon DNA damage. Within the MRN complex, MRE11 possesses both single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity. MRE11 first endonucleolytically cleaves the 5' strand at DNA DSB ends to prevent non-homologous end joining (NHEJ) and licence HR. It then generates a single-stranded DNA gap via 3' to 5' exonucleolytic degradation, which is required for single-strand invasion and recombination.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 17">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="16">Component of the MRN complex composed of two heterodimers RAD50/MRE11 associated with a single NBS1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 17">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 17 19">Belongs to the MRE11/RAD32 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D4K5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030870">
        <property type="term" value="C:Mre11 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008296">
        <property type="term" value="F:3'-5'-DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000014">
        <property type="term" value="F:single-stranded DNA endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042138">
        <property type="term" value="P:meiotic DNA double-strand break formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097552">
        <property type="term" value="P:mitochondrial double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007095">
        <property type="term" value="P:mitotic G2 DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00840">
        <property type="entry name" value="MPP_Mre11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.110.110:FF:000002">
        <property type="entry name" value="Double-strand break repair protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000019">
        <property type="entry name" value="Double-strand break repair protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.110">
        <property type="entry name" value="Mre11, capping domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003701">
        <property type="entry name" value="Mre11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038487">
        <property type="entry name" value="Mre11_capping_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007281">
        <property type="entry name" value="Mre11_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041796">
        <property type="entry name" value="Mre11_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00583">
        <property type="entry name" value="mre11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10139">
        <property type="entry name" value="DOUBLE-STRAND BREAK REPAIR PROTEIN MRE11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10139:SF1">
        <property type="entry name" value="DOUBLE-STRAND BREAK REPAIR PROTEIN MRE11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04152">
        <property type="entry name" value="Mre11_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000882">
        <property type="entry name" value="DSB_repair_MRE11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01347">
        <property type="entry name" value="Mre11_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="9 17 19" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12 17 19" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8 17 19" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="11 17 19" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="10 17 19" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13 17 19" id="KW-0464">Manganese</keyword>
    <keyword evidence="15 17 19" id="KW-0469">Meiosis</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 17 19" id="KW-0540">Nuclease</keyword>
    <keyword evidence="14 17 19" id="KW-0539">Nucleus</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mre11 DNA-binding" evidence="21">
        <location>
            <begin position="302"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="506"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="630"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="515"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="570"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="20">
        <location>
            <begin position="582"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="653"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="20">
        <location>
            <begin position="667"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="18">
        <location>
            <position position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00063862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRNR" id="PIRNR000882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR000882-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU003447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM01347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAJ9671753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="733" mass="81877" checksum="1F6E945322B9E4AE" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D5L4</accession>
    <name>A0AA39D5L4_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_026138</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D5L4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9672788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="231" mass="27063" checksum="14BED462DD28C2DE" modified="2024-01-24" version="1">MVEGYNFFMQPLVNIPRLNGKEDWRCISIFRTCVACQGRLCTLIIDGGDRRLSNPYQIAWVNDTFILVSSRCLVTSNFSNNFELLVWCDILMMKNTYTIVCNGCKKILHPMKEIPPLNQPKKKLAPLKLEEPSNTSIKKLKARGQPVEELKEVVEASEEPMLDFPRQNIIMSGGKREESTEISFEYREFKNFILLQDNLQEECWKQLSTSLLMTYIYFENYQLILQHWKVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D5M5</accession>
    <name>A0AA39D5M5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_027027</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D5M5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000888">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000396">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At2g36730"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000231">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000409">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000968">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24015">
        <property type="entry name" value="OS07G0578800 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24015:SF1993">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="18"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="119"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="222"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="323"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="393"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="424"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9670840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="637" mass="70453" checksum="083F062DA7EAE5E4" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D6Y7</accession>
    <name>A0AA39D6Y7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Calcium-transporting ATPase</fullName>
            <ecNumber evidence="17">7.2.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PVL29_025278</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Catalyzes the hydrolysis of ATP coupled with the transport of calcium.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 17">
            <text>Ca(2+)(in) + ATP + H2O = Ca(2+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18105"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 17">Membrane</location>
            <topology evidence="1 17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.10" evidence="17"/>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D6Y7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005388">
        <property type="term" value="F:P-type calcium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000039">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000006">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000013">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000193">
        <property type="entry name" value="Plasma membrane calcium-transporting ATPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006408">
        <property type="entry name" value="P-type_ATPase_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01517">
        <property type="entry name" value="ATPase-IIB_Ca"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093:SF434">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE 13, PLASMA MEMBRANE-TYPE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093">
        <property type="entry name" value="CATION TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 17" id="KW-0106">Calcium</keyword>
    <keyword evidence="4 17" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="11" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="14 17" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 17" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="143"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="299"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="352"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="767"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="796"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="901"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="931"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="20">
        <location>
            <begin position="58"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="18">
        <location>
            <begin position="178"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase C-terminal" evidence="19">
        <location>
            <begin position="792"/>
            <end position="954"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU361146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAJ9671497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="956" mass="103715" checksum="21BC7559F8665332" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D7C8</accession>
    <name>A0AA39D7C8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">alcohol dehydrogenase</fullName>
            <ecNumber evidence="4">1.1.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_023141</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a primary alcohol + NAD(+) = an aldehyde + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10736"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15734"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a secondary alcohol + NAD(+) = a ketone + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10740"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17087"/>
            <dbReference type="ChEBI" id="CHEBI:35681"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.1" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D7C8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051903">
        <property type="term" value="F:S-(hydroxymethyl)glutathione dehydrogenase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046294">
        <property type="term" value="P:formaldehyde catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.180.10:FF:000007">
        <property type="entry name" value="Alcohol dehydrogenase 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000003">
        <property type="entry name" value="S-(hydroxymethyl)glutathione dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43880">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43880:SF7">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE-LIKE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alcohol dehydrogenase-like N-terminal" evidence="13">
        <location>
            <begin position="37"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="12">
        <location>
            <begin position="208"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9673389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="384" mass="41952" checksum="8776B4B2DA716098" modified="2024-01-24" version="1">MMAGETRGKPIRCRAAVCRKAGEPLVMEEVMVSPPLAGEVRIRIICTSLCHSDITFWKLKDPPGCVPRILGHEAVGVVESVGENVSEVTEGDIVLPIFMPDCGECTDCRSEKSNLCSKFPFKISPWMPRYESSRFTDLKGEVLYHFLFVSTFSEYTVVDIANITKIDPNIPPNRACLFSCGVSTGVGAAWKTANVEKGSTVAIFGLGAIGLAVAEGARLCGASRIIGVDLNPDKFEIGKKFGVTDFVNSGNCGNKPVRQVIMEMTDGGADYCFECVGMASLVHEAFACCRKGWGKTIVLGVDRPGSMLSFPSFDILHSGKSLMGSLYGGVKPKSDTLILLKRYVDKELDVDKFVTHEMRFEDINKAFDLLIQGKSLRCVLWMDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D7D1</accession>
    <name>A0AA39D7D1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MGS-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_026140</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D7D1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003937">
        <property type="term" value="F:IMP cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004643">
        <property type="term" value="F:phosphoribosylaminoimidazolecarboxamide formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1380">
        <property type="entry name" value="Methylglyoxal synthase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011607">
        <property type="entry name" value="MGS-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036914">
        <property type="entry name" value="MGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002695">
        <property type="entry name" value="PurH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11692:SF0">
        <property type="entry name" value="BIFUNCTIONAL PURINE BIOSYNTHESIS PROTEIN ATIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11692">
        <property type="entry name" value="BIFUNCTIONAL PURINE BIOSYNTHESIS PROTEIN PURH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02142">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00851">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52335">
        <property type="entry name" value="Methylglyoxal synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MGS-like" evidence="2">
        <location>
            <begin position="6"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="104"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9672790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="150" mass="16917" checksum="61A368275182636B" modified="2024-01-24" version="1">MMAKVDTISILKARSQSTALGNKQGVSATKVEQLTCFPEMLDGRVKTLHLSIHGGILARRDQKHHMEALNEHGIGTFDVVVVVNLYPFHDEVFSGGIEFEDEIETQAQDFDEPVKQSREGHDAQQPKLTIEGMKMIVEYKIQRKKNDDPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D856</accession>
    <name>A0AA39D856_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">Laccase</fullName>
            <ecNumber evidence="4 13">1.10.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Benzenediol:oxygen oxidoreductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Diphenol oxidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Urishiol oxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_024359</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Lignin degradation and detoxification of lignin-derived products.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>4 hydroquinone + O2 = 4 benzosemiquinone + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:11276"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17594"/>
            <dbReference type="ChEBI" id="CHEBI:17977"/>
            <dbReference type="EC" id="1.10.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="13">Binds 4 Cu cations per monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Secreted</location>
            <location evidence="2 13">Extracellular space</location>
            <location evidence="2 13">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the multicopper oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.10.3.2" evidence="4 13"/>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D856"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052716">
        <property type="term" value="F:hydroquinone:oxygen oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046274">
        <property type="term" value="P:lignin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13849">
        <property type="entry name" value="CuRO_1_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13875">
        <property type="entry name" value="CuRO_2_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13897">
        <property type="entry name" value="CuRO_3_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033138">
        <property type="entry name" value="Cu_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002355">
        <property type="entry name" value="Cu_oxidase_Cu_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034288">
        <property type="entry name" value="CuRO_1_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034285">
        <property type="entry name" value="CuRO_2_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034289">
        <property type="entry name" value="CuRO_3_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017761">
        <property type="entry name" value="Laccase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03389">
        <property type="entry name" value="laccase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF261">
        <property type="entry name" value="LACCASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00079">
        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00080">
        <property type="entry name" value="MULTICOPPER_OXIDASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0052">Apoplast</keyword>
    <keyword evidence="10 13" id="KW-0186">Copper</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 13" id="KW-0439">Lignin degradation</keyword>
    <keyword evidence="7 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 13" id="KW-0964">Secreted</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041486687" description="Laccase" evidence="13">
        <location>
            <begin position="30"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="16">
        <location>
            <begin position="39"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="14">
        <location>
            <begin position="166"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="15">
        <location>
            <begin position="441"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9675398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="595" mass="66845" checksum="F2EFD40A4B3CFAC5" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D8A8</accession>
    <name>A0AA39D8A8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">sterol 3beta-glucosyltransferase</fullName>
            <ecNumber evidence="2">2.4.1.173</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_024472</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 28 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.173" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D8A8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016906">
        <property type="term" value="F:sterol 3-beta-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010154">
        <property type="term" value="P:fruit development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009791">
        <property type="term" value="P:post-embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016126">
        <property type="term" value="P:sterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000009">
        <property type="entry name" value="Sterol 3-beta-glucosyltransferase UGT80A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000030">
        <property type="entry name" value="Sterol 3-beta-glucosyltransferase UGT80A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010610">
        <property type="entry name" value="EryCIII-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050426">
        <property type="entry name" value="Glycosyltransferase_28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004276">
        <property type="entry name" value="GlycoTrans_28_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48050">
        <property type="entry name" value="STEROL 3-BETA-GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48050:SF16">
        <property type="entry name" value="STEROL 3-BETA-GLUCOSYLTRANSFERASE UGT80B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06722">
        <property type="entry name" value="EryCIII-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03033">
        <property type="entry name" value="Glyco_transf_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="7" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="9" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase family 28 N-terminal" evidence="11">
        <location>
            <begin position="179"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="Erythromycin biosynthesis protein CIII-like C-terminal" evidence="12">
        <location>
            <begin position="475"/>
            <end position="573"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF06722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9675563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="631" mass="69683" checksum="AC363DF8B5938052" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D8C1</accession>
    <name>A0AA39D8C1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Disease resistance protein RGA3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_026281</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D8C1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056789">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766:SF38">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766">
        <property type="entry name" value="PLANT BROAD-SPECTRUM MILDEW RESISTANCE PROTEIN RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25019">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0611">Plant defense</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1072"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance N-terminal" evidence="8">
        <location>
            <begin position="12"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="7">
        <location>
            <begin position="166"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="9">
        <location>
            <begin position="423"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="R13L1/DRL21-like LRR repeat region" evidence="10">
        <location>
            <begin position="662"/>
            <end position="791"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF25019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9672952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1130" mass="127826" checksum="37ECA7FC7A8AEDB6" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D8V0</accession>
    <name>A0AA39D8V0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA/RNA-binding protein Alba-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_023188</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histone-like Alba family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D8V0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.110.20:FF:000003">
        <property type="entry name" value="DNA/RNA-binding protein Alba 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.20">
        <property type="entry name" value="Alba-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036882">
        <property type="entry name" value="Alba-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051958">
        <property type="entry name" value="Alba-like_NAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002775">
        <property type="entry name" value="DNA/RNA-bd_Alba-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13516:SF14">
        <property type="entry name" value="ALBA DNA_RNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13516">
        <property type="entry name" value="RIBONUCLEASE P SUBUNIT P25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01918">
        <property type="entry name" value="Alba"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82704">
        <property type="entry name" value="AlbA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA/RNA-binding protein Alba-like" evidence="5">
        <location>
            <begin position="19"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="141"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="151"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9673477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="188" mass="21051" checksum="241BAC61595C32D9" modified="2024-01-24" version="1">MDRYQRVEKPREETPIDENEIRITSQGRMRSYITYAMSLLQEKGSNEIVFKAMGRAINKTVTIVELIKRRIVGLHQNTSIGSTDITDTWEPLEEGLLPLETTRHVSMITITLSKKELNTSSIGYQPPLPAEQVKAWTEFDYEGEGSPSGRGRGRGGRGRGRTRGVSGMISNCMPSLSRLTGDSLCMRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D9D3</accession>
    <name>A0AA39D9D3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transposase MuDR plant domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_024670</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D9D3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31973:SF187">
        <property type="entry name" value="MUTATOR TRANSPOSASE MUDRA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31973">
        <property type="entry name" value="POLYPROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9675836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="344" mass="39270" checksum="21C6737019AB4866" modified="2024-01-24" version="1">MLFQLGYKDCMSYYYANKNGELSWCLTDGNVLEMCSSLSKDGGVEVFVKHREVLHIAQGEIGSSNVCNQLGESVCEGEDYTTNVEPKPNVDQTPQYEDKGGLDYEEAERDSFGDSDFIDDEYPVSDDDDLYDNYVDANEEWVGVKGKEDKEKGVEGEYTIMNDVEDEKNISCYEDAIKNGRNVKFVKNEKDKVRVVCSKGCPWVVYVAEVRDEKKFQVRTYNGQHTCSRVLKNDNVTLRYLCNKYVDHFRNNPKLSIGAFMNIVRTSLACEVSPSQAYRTKRKTLKQIQGTYVDQYVKLPDYCAEMRRTNSGSTVILKTELVEDKGLLPAIEKLLPNSKHKYCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DAN4</accession>
    <name>A0AA39DAN4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DYW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_022823</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. PCMP-H subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9678053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DAN4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000184">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032867">
        <property type="entry name" value="DYW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF461">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14432">
        <property type="entry name" value="DYW_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="59"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="249"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="DYW" evidence="4">
        <location>
            <begin position="464"/>
            <end position="556"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9678053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="556" mass="62771" checksum="6D586808DD88C938" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DBG6</accession>
    <name>A0AA39DBG6_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_020913</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DBG6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34271">
        <property type="entry name" value="NUCLEOLAR HISTONE METHYLTRANSFERASE-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34271:SF1">
        <property type="entry name" value="NUCLEOLAR HISTONE METHYLTRANSFERASE-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9678863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="186" mass="20540" checksum="41FEB32FFD93A4B8" modified="2024-01-24" version="1">MSQDASNDPPHGVGCSRVFKSHLVHTLVHRLVEKKKPLIRNSNLCISLVHIKVQQNGAFHPKNGDSPEGNARDDNDTLSVAMPSTGVEDVASQINEIGSEDCNPPVKEEKTNERDISLDQNPLHSLPHLISSSAPINSLLMQRRMHFCGWISDNEPNDICTKQLIPAIVMQLEKVICGTNTHSKSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DCK4</accession>
    <name>A0AA39DCK4_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_020730</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DCK4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9678640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="144" mass="16570" checksum="7EBEFC6326330B00" modified="2024-01-24" version="1">MDHQTDQRWQVQQLTNISEVCGNGSSHQEQMTACLPSMVELSHLLQLQKFLQIVLSSADFIQNQMQSGYLSRSHREETGFKAAGTLHPLQEVTKTLNNRGPQKVLRESRMYLRIQHQQDSLNFIPRLHFIMATLLHTPPPLLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DFV8</accession>
    <name>A0AA39DFV8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_019116</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000440">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025724">
        <property type="entry name" value="GAG-pre-integrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054722">
        <property type="entry name" value="PolX-like_BBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057670">
        <property type="entry name" value="SH3_retrovirus"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439">
        <property type="entry name" value="GAG-POL-RELATED RETROTRANSPOSON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439:SF484">
        <property type="entry name" value="REVERSE TRANSCRIPTASE TY1_COPIA-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13976">
        <property type="entry name" value="gag_pre-integrs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22936">
        <property type="entry name" value="Pol_BBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25597">
        <property type="entry name" value="SH3_retrovirus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="3">
        <location>
            <begin position="484"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="209"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="254"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="210"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="267"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9683393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1343" mass="149173" checksum="6C825DB6642434AB" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DHU2</accession>
    <name>A0AA39DHU2_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_017462</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DHU2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="369"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="415"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="198"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="227"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9685433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="477" mass="53164" checksum="BD7BFBBB3C96A651" modified="2024-01-24" version="1">MYPNVVLPALLEVEDGAWTFTVAISVTREVDEDDSITPESDRCRDGLVNMGGYVSQRSKNAEGLCGSNRDNECYSRRPLLQPRNRFSSSKLASKRENGWGGRLLGPEEERFIGLTVSEPLNKAQPVRAQSGTIFCGPEVGLVASQAHVTVALSSSSRQQAVSSAKAIPLLASTHGLVEGRADVVDLSGEDGWAFEIKAQSPPILSPPSDDVVGCDLKGSSRLGQSLKGKEEEDDAALRGEAPEGQLRGSSQKEELVVTKRMWTTLFPPSSDRRKERRSRSEPIFPAKPPLVSEARPLEKAQRFLSFAVICEIGGRGKGPRRQVVQWLCAINASKKTKKSWVHFLVQKSEVKGSSLRGFANFWEWKIRRYVCLLILSLPSLLLPLIVVCFFLWKIGSLCFFLPQSIFLSLLWKIEFFLKFWLIKTLLAIFFKIILLFQTRCQSVLHPVSLNLACPRRFLIGIQAARAILWSPHRENSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DI04</accession>
    <name>A0AA39DI04_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Nodulin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_016928</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DI04"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd17354">
        <property type="entry name" value="MFS_Mch1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056555">
        <property type="entry name" value="NFD4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010658">
        <property type="entry name" value="Nodulin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21576:SF84">
        <property type="entry name" value="FAMILY PROTEIN, PUTATIVE, EXPRESSED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21576">
        <property type="entry name" value="UNCHARACTERIZED NODULIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23262">
        <property type="entry name" value="NFD4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06813">
        <property type="entry name" value="Nodulin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="28"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="92"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="157"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="189"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="221"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="253"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="347"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="394"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="425"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="450"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="484"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="539"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="domain" description="Nodulin-like" evidence="6">
        <location>
            <begin position="27"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="NFD4 C-terminal" evidence="7">
        <location>
            <begin position="351"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF06813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9684699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="599" mass="65411" checksum="C0159EFDC7D99809" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DIG9</accession>
    <name>A0AA39DIG9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Receptor-like serine/threonine-protein kinase</fullName>
            <ecNumber evidence="19">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="ORF">PVL29_015106</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="26">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="26">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="19"/>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DIG9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00028">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.90.10.10:FF:000039">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase SD2-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.90.10.30:FF:000003">
        <property type="entry name" value="Os04g0303100 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000248">
        <property type="entry name" value="S-receptor-like kinase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000178">
        <property type="entry name" value="serine/threonine-protein kinase PBS1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051343">
        <property type="entry name" value="G-type_lectin_kinases/EP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024171">
        <property type="entry name" value="SRK-like_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47976">
        <property type="entry name" value="G-TYPE LECTIN S-RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD2-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47976:SF30">
        <property type="entry name" value="RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000641">
        <property type="entry name" value="SRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 19 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 19" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0430">Lectin</keyword>
    <keyword evidence="13 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 19 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 19" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7 22" id="KW-0732">Signal</keyword>
    <keyword evidence="5 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041398424" description="Receptor-like serine/threonine-protein kinase" evidence="22">
        <location>
            <begin position="27"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="469"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="24">
        <location>
            <begin position="53"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="25">
        <location>
            <begin position="358"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="526"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="554"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRNR" id="PIRNR000641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EMBL" id="KAJ9686063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="845" mass="93850" checksum="13B9ADCFF949A321" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DIU4</accession>
    <name>A0AA39DIU4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cellulose synthase-like protein H1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_015392</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DIU4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016760">
        <property type="term" value="F:cellulose synthase (UDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030244">
        <property type="term" value="P:cellulose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005150">
        <property type="entry name" value="Cellulose_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13301">
        <property type="entry name" value="X-BOX TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03552">
        <property type="entry name" value="Cellulose_synt"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="active site" evidence="8">
        <location>
            <position position="71"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="340"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR605150-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR605150-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9686468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="569" mass="63471" checksum="CC2110E06B6DFA01" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DJV0</accession>
    <name>A0AA39DJV0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glucosamine inositolphosphorylceramide transferase 1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_016958</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DJV0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056442">
        <property type="entry name" value="GINT1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052176">
        <property type="entry name" value="Glycosyl_Hydrlase_43_Enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43772">
        <property type="entry name" value="ENDO-1,4-BETA-XYLANASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43772:SF2">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_2G04480)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24793">
        <property type="entry name" value="GINT1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110296">
        <property type="entry name" value="Oligoxyloglucan reducing end-specific cellobiohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="14"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="domain" description="Glucosamine inositolphosphorylceramide transferase 1 N-terminal" evidence="3">
        <location>
            <begin position="76"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9684742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="147" mass="16648" checksum="30DA1EB8231BF4FA" modified="2024-01-24" version="1">MGVWDYHCFLSSTFVFFISSFVVYGFIAAVYAWLFVNPHAPLELASLGCRPDSEGSWAIGVFYGDSPFSLRPIEAGDALFLFYETKNSITMQGDIGVSKSDDKGATWQHLGVALDEEWHLSYPYVFEYLGKVRFQFHRNTLCSINIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DK41</accession>
    <name>A0AA39DK41_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_015928</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DK41"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9687251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="72" mass="7230" checksum="FF294281B5D2C164" modified="2024-01-24" version="1">MATLIGVEVGADPGARASTVSTATWNMRDMGGGAASSPGLDRGSMTGTKTNSVRLAAIFGFSGLRRISSCHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DKE1</accession>
    <name>A0AA39DKE1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_015870</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DKE1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33527:SF18">
        <property type="entry name" value="F13O11.17 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33527">
        <property type="entry name" value="OS07G0274300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9687189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="279" mass="31354" checksum="EE758DD0C86F30A5" modified="2024-01-24" version="1">MAFKIPLRELLSFHSVDRQIFSRLILNCLRKPSETLLVMAMWLWLEEIGYPNIVAKILILPDSIVNAIANEAVLCLKCLSSDSPPPRLPSGNLPLSTRAMEMEISLQMFFQNKFTAISGIKKFLNSVCAWAFTDILVQVLPSASQVLLNQPVAVPGFPHPLFGNVTIVLRSLDYSFPIGGLHGWNPTTEAPVDDRTMFLTFSRGNKVSESDVRELFTGLFGDCVDSVNMEEVSRNRQPLFARLVLRSVSTVDRILNGSSIAKFAINSKHIWARKYERRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DMD5</accession>
    <name>A0AA39DMD5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Cysteine-rich receptor-like protein kinase 29</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">PVL29_012646</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="23">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DMD5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009737">
        <property type="term" value="P:response to abscisic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23509">
        <property type="entry name" value="Gnk2-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000129">
        <property type="entry name" value="cysteine-rich receptor-like protein kinase 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000142">
        <property type="entry name" value="Cysteine-rich receptor-like protein kinase 10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.430.20:FF:000002">
        <property type="entry name" value="Cysteine-rich receptor-like protein kinase 10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000343">
        <property type="entry name" value="Cysteine-rich receptor-like protein kinase 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.430.20:FF:000003">
        <property type="entry name" value="Cysteine-rich RLK (RECEPTOR-like protein kinase) 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.430.20">
        <property type="entry name" value="Gnk2 domain, C-X8-C-X2-C motif"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002902">
        <property type="entry name" value="GNK2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038408">
        <property type="entry name" value="GNK2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002:SF1104">
        <property type="entry name" value="CYSTEINE-RICH RECEPTOR-LIKE PROTEIN KINASE 27-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002">
        <property type="entry name" value="RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD1-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01657">
        <property type="entry name" value="Stress-antifung"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51473">
        <property type="entry name" value="GNK2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="12 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 20" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041441299" description="Cysteine-rich receptor-like protein kinase 29" evidence="20">
        <location>
            <begin position="26"/>
            <end position="1181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="274"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="820"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="22">
        <location>
            <begin position="29"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="22">
        <location>
            <begin position="133"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="332"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="22">
        <location>
            <begin position="558"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="22">
        <location>
            <begin position="667"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="877"/>
            <end position="1175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="243"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="783"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="245"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="783"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="360"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="905"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAJ9690098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="25">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1181" mass="131109" checksum="058B10939F2CE1E1" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DQI8</accession>
    <name>A0AA39DQI8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">ADP/ATP translocase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">ADP,ATP carrier protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_012296</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">ADP:ATP antiporter that mediates import of ADP into the mitochondrial matrix for ATP synthesis, and export of ATP out to fuel the cell. Cycles between the cytoplasmic-open state (c-state) and the matrix-open state (m-state): operates by the alternating access mechanism with a single substrate-binding site intermittently exposed to either the cytosolic (c-state) or matrix (m-state) side of the inner mitochondrial membrane.</text>
    </comment>
    <comment type="function">
        <text evidence="13">Catalyzes the exchange of ADP and ATP across the membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ADP(in) + ATP(out) = ADP(out) + ATP(in)</text>
            <dbReference type="Rhea" id="RHEA:34999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:35000"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="3 13">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Membrane</location>
            <topology evidence="13">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9693461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DQI8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005471">
        <property type="term" value="F:ATP:ADP antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140021">
        <property type="term" value="P:mitochondrial ADP transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990544">
        <property type="term" value="P:mitochondrial ATP transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002113">
        <property type="entry name" value="ADT_euk_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45635">
        <property type="entry name" value="ADP,ATP CARRIER PROTEIN 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45635:SF14">
        <property type="entry name" value="ADP_ATP TRANSLOCASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 13" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU368008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9693461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="214" mass="23373" checksum="6B5316204983BEDD" modified="2024-01-24" version="1">MADGSQYSLIFHKINGQSYLFSTLSPNLQSRNTSVYSLSSVCVNVGLQTSLLPTSSGNGLALVSPLSPIFAQAPAEKGTKVSAVVSKSTAAPIERVKLLIQNQDEIIKAGRLSELYKGIIDHFARTIKDENIFPYSGPELHFQGLLQETLQFDGYWKWFAGNLVSGGAAGASSLLFVYSLDYVCTSLANDAKAMCHLFFFFFSSSFDLKNLIFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DSS0</accession>
    <name>A0AA39DSS0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EMC1 first beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_011785</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DSS0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000579">
        <property type="entry name" value="Os05g0230600 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058545">
        <property type="entry name" value="Beta-prop_EMC1_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026895">
        <property type="entry name" value="EMC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21573">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21573:SF0">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25293">
        <property type="entry name" value="Beta-prop_EMC1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041399502" description="EMC1 first beta-propeller domain-containing protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="EMC1 first beta-propeller" evidence="2">
        <location>
            <begin position="23"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9692857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="488" mass="54441" checksum="13F076E484BB3F7A" modified="2024-01-24" version="1">MATRVFLLLLLVLISSPSPTFSLYEDQVGLMDWHQQYIGKVKHAVFHTQKAGRKRVVVSTEENVIASLNLRRRDFFWRHVLGPNDSVDEIDIALGKYVITLSSEGSILKAWNLRDGQMAWESFLQGPKPSKSLLSVPANLKIDKDNVIFVFGKECLHAVSSIDGEVLWKKDFADESIEVQQIIHPLGSDMIYAVGFVGLSQLDAYQINVRNGEVLKHRSAAFPGGFCGEVSLVSNDALVALDATKSCLISISFLDGEISIQQTHISNLVGDSFGMAVMLPSKLSRMLMIKINNYIVFVRVADEGKLEVAEKINDAAAVSDALTLSEGQQAFGLAEHGSNKIHLIVKLVNDWNGDLLKESIGMDHQRGCVHKIFINNYIRTDRSYGFRVLIVMEDHSLLLLQQGEIVWSREDGLASIINVTASELPLEKEGVSVAKVEHNLFEWLWNQLNMGSRTQKGTLRFPGFGRTKVIMNSLDPAWIKKNYCCLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DT68</accession>
    <name>A0AA39DT68_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_008653</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRM U1 A/B'' family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DT68"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030532">
        <property type="term" value="C:small nuclear ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12246">
        <property type="entry name" value="RRM1_U1A_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12247">
        <property type="entry name" value="RRM2_U1A_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000039">
        <property type="entry name" value="U1 small nuclear ribonucleoprotein A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000029">
        <property type="entry name" value="U2 small nuclear ribonucleoprotein B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10501">
        <property type="entry name" value="U1 SMALL NUCLEAR RIBONUCLEOPROTEIN A/U2 SMALL NUCLEAR RIBONUCLEOPROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="14"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="171"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="107"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="109"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9696536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="245" mass="27402" checksum="033384865C75D455" modified="2024-01-24" version="1">MAELNTGGEISPNMTIYINNLNEKIKLEELKKSLFAVFSQFGKIMEVLAFKTLKHKGQAWVVFEEVSSATNALRQMQGFPFYDKPMRIQYAKTKSDVIAKADGTFVPRERRRRHDDRAGRKRREQHDANQAGVGVNPAYGGAYGAAPPLSQIPYLGGAKSAVPEAPAPPNNILFIQNLPHEATPMMLQMFFCQYPGFKEVRMVEAKPGIAFVEYGDEMQSTVAMQGLQGLKINQQNPMLITYAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DUB9</accession>
    <name>A0AA39DUB9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Retrovirus-related Pol polyprotein from transposon TNT 1-94</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_009164</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DUB9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47481">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47481:SF22">
        <property type="entry name" value="RETROTRANSPOSON GAG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9697253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="119" mass="13409" checksum="D58A0C6664CC357E" modified="2024-01-24" version="1">MAQIIGLDTASEIWTTLEKIFSAASKARIMQLRFQLQTTKKGGLSMMEYLLKIKSIVDNLLAIGENITEQDRILYLLAGLGAEYNSFVISVTSRHEPLSLEEIHSMLLTHENQLNNNTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DVH1</accession>
    <name>A0AA39DVH1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GST C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_008991</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DVH1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03190">
        <property type="entry name" value="GST_C_Omega_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000045">
        <property type="entry name" value="Glutathione S-transferase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000265">
        <property type="entry name" value="Glutathione S-transferase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047047">
        <property type="entry name" value="GST_Omega-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016639">
        <property type="entry name" value="GST_Omega/GSH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32419">
        <property type="entry name" value="GLUTATHIONYL-HYDROQUINONE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32419:SF31">
        <property type="entry name" value="OS02G0814800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13410">
        <property type="entry name" value="GST_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13409">
        <property type="entry name" value="GST_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01206">
        <property type="entry name" value="Xi.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01148">
        <property type="entry name" value="Xi_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST C-terminal" evidence="1">
        <location>
            <begin position="238"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9697030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="408" mass="45670" checksum="8B28B324FE4CF0F0" modified="2024-01-24" version="1">MSSCSANALLTPSLPITRKKKKRHVIITFTTSRSHVSPRMSLQQPLTAITKLLWGNSLPPQLLISTVRSTWSATWHLFMSQLAPSDPSGRYSRPPSQFPANFNSLQFSRQNPSSLHLYVALPCPWAHRTLIVRALKGLENAIPVSIAAPGTDGSWEFKDGAAPDGDTFVPGLDKANGCRTLREVYRLRRGGYNGRSTVPMLWDVEKKEVVCNESYDIIQLFNSGLNELASNPALDLAPPALKGRIEEWNRVIYPGVNNGVYRCGFAQSQEAYDTAVNELFTTLDMLDDHLGRCRYLCGDMLTLADICLFTTLVRFDLVYNVLFKCTKKKLLEYPNLHGYMRDIYQIPKVAATCNFQAIMDGYYGILFPLNPGNIRPTMPSGCEHEALIRPHNRESLSSVDQRMGVHVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DW09</accession>
    <name>A0AA39DW09_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DDE Tnp4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_008391</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HARBI1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DW09"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035098">
        <property type="term" value="C:ESC/E(Z) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035102">
        <property type="term" value="C:PRC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045249">
        <property type="entry name" value="HARBI1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930:SF85">
        <property type="entry name" value="GH03217P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE Tnp4" evidence="8">
        <location>
            <begin position="207"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9696127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="428" mass="48248" checksum="6F2BEC6CDE37AA74" modified="2024-01-24" version="1">MAPPKKSKKAKRELKKSKKCKSMSVVPIEPRATESDWWDSFWQKNSPIPVFSATKWRNECNPFKNASVMKILYTVSDCSAIPTDEAEGFKFFFRVSKKTFDYICSLVRQDLVSRPPSGLINIEGRLLSVEKQVAIALRRLASGESQVSVGAAFGVGQSTVSQVTWRFIEAVEERAKHHLRWPDFNRMEEIKSKFETSYGLSNCCGAIDATHIIMTLPAVQTSDDWCDQENNYSMFLQGVVDDEMRFLDIVTGWPGGMTVARLLKCTGFYRNCEAGERLNGNIRILSEGVEIREYVVGGVGYPLLPWLITPCEYNDISSSMSGFKTNHEAARSLAVRAFSQLKGTWRILSKVMWRPDKRKLPSIILVCCLLHNIIIDSKDKLQPDVALSSHHDPGYGQQCCKQVDPLGKAMRENIAKHLQHGKEKAPSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DWR0</accession>
    <name>A0AA39DWR0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dirigent protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_007415</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Secreted</location>
            <location evidence="4">Extracellular space</location>
            <location evidence="4">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the plant dirigent protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DWR0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009699">
        <property type="term" value="P:phenylpropanoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.480.10">
        <property type="entry name" value="Allene oxide cyclase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044859">
        <property type="entry name" value="Allene_oxi_cyc_Dirigent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004265">
        <property type="entry name" value="Dirigent"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46215:SF5">
        <property type="entry name" value="DIRIGENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46215">
        <property type="entry name" value="DIRIGENT PROTEIN 24-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03018">
        <property type="entry name" value="Dirigent"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0052">Apoplast</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041487149" description="Dirigent protein" evidence="4">
        <location>
            <begin position="29"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9698320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="300" mass="30750" checksum="52A16AE5B1414336" modified="2024-01-24" version="1">MANHMLVSVPFKAISFLFLALAIPCAISARILDEEAPITPAVAPEASDLFETPVSSVTSVATASAAATSTTVTNTDLHHTLSFFMHLGGSNPSAGGVTGIVTNPAVNGQVPFAKSNGAVLPVNNGVPLNNGNSALINNNNIPFLTGLSGNTANVIQNNGNNVINGGSGLPVFNGGQFPAGTNLQKLMFGTMTVIDDQLIEGHELGSGLVGKAQGFYLASSEDGSSQTMAFTAMFESDGYASSLSFFGAHRTAVSESQLAIMGGTGKYVNAEGYATVKTIPGANHTDGTETLLQIIVYLAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DXW1</accession>
    <name>A0AA39DXW1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SHSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_004005</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DXW1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.790:FF:000010">
        <property type="entry name" value="17.3 kDa class II heat shock protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031107">
        <property type="entry name" value="Small_HSP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11527">
        <property type="entry name" value="HEAT-SHOCK PROTEIN 20 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="6">
        <location>
            <begin position="40"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9702056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="156" mass="17321" checksum="C5348781BE6F0AD1" modified="2024-01-24" version="1">MDLRIMGFDSPLFSTLQHMLDAADDSEKSVSAPTSTYVRDAKAMAATPADVKEYPNSYTFIVDMPGLKSGDIKVQVEDGNVLVISGERKREEEKEGAKYVRMERRVGKFMRKFVLPENANTDKISAVCQDGVLTVTVEKLPPPEPKKPKTIEVKIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DYH1</accession>
    <name>A0AA39DYH1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_004454</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DYH1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.150.80:FF:000021">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.80">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003441">
        <property type="entry name" value="NAC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036093">
        <property type="entry name" value="NAC_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31719">
        <property type="entry name" value="NAC TRANSCRIPTION FACTOR 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31719:SF179">
        <property type="entry name" value="OS08G0148400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02365">
        <property type="entry name" value="NAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101941">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51005">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NAC" evidence="6">
        <location>
            <begin position="35"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="194"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9702731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="461" mass="52464" checksum="320FEA48BE483667" modified="2024-01-24" version="1">MNLLQLGEDASLQTAPQEVQNNHSEDGKHFDLNSLPPGYRFNPTDAEIIVFYLKKKVDHQPLPPNKIIEVNLYAYDPEELAARYKPSGNDEWFYFTPRDRKYRNGQRPNRSVEGGYWKATGADKEIKFEDRTVGYRKVLVFYRGSPPKGVKTNWIMHEFRVAEPPEPPRRTGVNDMRLDECVLCKVYRKDKRVCKRSRHESGVDESGSPRPKTPRFDRHPNPNEANNQHTVGGGMDSGEDRNGYQYNQNINSHQALQNQRQLQQPIIQMPTANAVFPQDNLQVLPPAGTDVSFHGHNSNRIHQALQNQQQLQLQLQQPIIQMPTANAVFPQDNLQVLPPAGTDVSFHGHNSNLIHQALQNQQQQQLQLQQPIIQRPTANAVFPQDNLQALPPAGTDVSFHGHNSNLMQSMEYPFPGHPWGMYSEFHNTGFASTMDLKPGYMTGVNIDPNTRSASFSSPRLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E0W7</accession>
    <name>A0AA39E0W7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tr-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_004823</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E0W7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001857">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050100">
        <property type="entry name" value="TRAFAC_GTPase_members"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23115">
        <property type="entry name" value="TRANSLATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="3">
        <location>
            <begin position="5"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9703194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="121" mass="13787" checksum="E7E2011E708D62D4" modified="2024-01-24" version="1">MGKGKVHVNIVVIDHVDHGKLTITGNLIYKLGGIDKRVIEQFEKEVIEMNQRSFKYAWVLDKLKAEREHGITIDIALWKFGTTKYYYIVIDAHGHWDFIKNVVTSTSQASCLVLIINSTIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E273</accession>
    <name>A0AA39E273_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HVA22-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_003508</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E273"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004345">
        <property type="entry name" value="TB2_DP1_HVA22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12300:SF43">
        <property type="entry name" value="HVA22-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12300">
        <property type="entry name" value="HVA22-LIKE PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03134">
        <property type="entry name" value="TB2_DP1_HVA22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="244"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="192"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="192"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU362006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9705503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="277" mass="31263" checksum="F6FF9F35ED7F24A4" modified="2024-01-24" version="1">MGVMNVLKFSAACFDLLGWPLFALGYPLCSSIWVIETNSISDIRKLVTYWVLFSLISLFDHAFAKLLEWIPFWPYIKLMIICCLVMPHFDGSYYVYKHLCEELNVAQKKIQVVELTEIKNGDGAKRCEGPNVAKEEIKVTKAAQRTFGGTAKWVKRTELNLGETKSTTTCAEEIAERATKVEQKKRTFNNLKAKNNGISASSTVKKPAEQHKSASSNGLEQKNNKSHQKNLKQSGGGMKEWGSWCNICNVSRTSELDMASHLNGRRHFNRMNYNAAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E460</accession>
    <name>A0AA39E460_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_000044</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9707783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E460"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044605">
        <property type="entry name" value="At1g26460-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47205">
        <property type="entry name" value="OS07G0599000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47205:SF1">
        <property type="entry name" value="OS07G0599000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13812">
        <property type="entry name" value="PPR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="179"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="493"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="528"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="46"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9707783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="616" mass="69261" checksum="200E8D93D7FA9C33" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E587</accession>
    <name>A0AA39E587_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_002554</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E587"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33325:SF5">
        <property type="entry name" value="TRANSCRIPTION FACTOR INTERACTOR AND REGULATOR CCHC(ZN) FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33325">
        <property type="entry name" value="ZINC FINGER, CCHC-TYPE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="90"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="26"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="74"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9707574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="163" mass="18740" checksum="EE527A0B19178F98" modified="2024-01-24" version="1">MRNHQSRPTGSEPFPEVNAISSQTRGRGRGRGRGRGRGRGRNPRYHGSYSNNSQKMKASLHHQKWNNTETIQENGKRLQDKPPKNHENNCYRCGMKGHWSRTCRTPKHLVDLYQASIKAKGKEIEMNFTDGDGLDLTYYDIDFFGGPNEKTDHLINDKKINID</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E8M7</accession>
    <name>A0AA39E8M7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1664 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_001390</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E8M7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012458">
        <property type="entry name" value="DUF1664"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47289">
        <property type="entry name" value="TRANSCRIPTION FACTOR, PUTATIVE (DUF1664)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47289:SF2">
        <property type="entry name" value="TRANSCRIPTION FACTOR, PUTATIVE (DUF1664)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07889">
        <property type="entry name" value="DUF1664"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041451830" description="DUF1664 domain-containing protein" evidence="2">
        <location>
            <begin position="23"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1664" evidence="3">
        <location>
            <begin position="92"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="234"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="310"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="234"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="266"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="321"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9709884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="361" mass="38526" checksum="92922875BC9D9039" modified="2024-01-24" version="1">MALPLGKLTLLVGAGIVGSVLAKEGRMSDVSNFFSGAFKIALKQLKQDDSTSPTVKPKNDALLAQVNSLRQELQILASNRSITIVTASGTGKSKYGVVVIIVVVGYGYAWWKGWKLPNMMFATRRSLSDACSSIAKQLENVYSSIAATKRHLSSRIDRVDCSIDEFAELTSATKEEVFELRGGMKMIGGDVASVQKAVQNLESKIIEIEGKQDITNEGLGRLCHYAWNLENSRTTERIQASPSSSFRPALESRQTTPPLRTGSLPPMVPSLEPPPSPSNPSNSTGSPKPPLQNAALASGLKELDGISKAAETTNTPEVSNGIGGLEETGNGSSGSGLFGMRLSYPSFITRTRSATQAFATK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YJ42</accession>
    <name>A0AA38YJ42_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA polymerase delta subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_025209</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YJ42"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043625">
        <property type="term" value="C:delta DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904161">
        <property type="term" value="P:DNA synthesis involved in UV-damage excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006297">
        <property type="term" value="P:nucleotide-excision repair, DNA gap filling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1030.20:FF:000002">
        <property type="entry name" value="DNA polymerase delta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1030.20">
        <property type="entry name" value="DNA polymerase delta, p66 (Cdc27) subunit, wHTH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019038">
        <property type="entry name" value="POLD3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041913">
        <property type="entry name" value="POLD3_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17598">
        <property type="entry name" value="DNA POLYMERASE DELTA SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17598:SF13">
        <property type="entry name" value="DNA POLYMERASE DELTA SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09507">
        <property type="entry name" value="CDC27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="153"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="285"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="172"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="318"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="364"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="390"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="437"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="455"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9671401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="524" mass="56697" checksum="7830D9DD10D97610" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YK28</accession>
    <name>A0AA38YK28_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Response regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_025521</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YK28"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052048">
        <property type="entry name" value="ST_Response_Regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43228:SF17">
        <property type="entry name" value="HISTIDINE KINASE RESPONSE REGULATOR AND TRANSCRIPTION FACTOR RR-A-TYPE FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43228">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="70"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="33"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9671871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="150" mass="16221" checksum="68F6AABDC7F95F06" modified="2024-01-24" version="1">MELPSKINNNEKSLQQGISPSGNGGLISNSKLTALVVDSDRICQILEKAILESQGVETQVVGSGRDALDLLASGATFNLIIIAMVLPIMNGVETTRQIRAMGIRSKMLGVTACSYEREKQAFLDAGVDEFIEKPLTPQKLASIMREMRNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YK88</accession>
    <name>A0AA38YK88_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SHSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_025564</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YK88"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47838">
        <property type="entry name" value="21.7 KDA CLASS VI HEAT SHOCK PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47838:SF1">
        <property type="entry name" value="21.7 KDA CLASS VI HEAT SHOCK PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SHSP" evidence="3">
        <location>
            <begin position="77"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9671972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="192" mass="22345" checksum="74564B7F21DA9B4B" modified="2024-01-24" version="1">MTSSKQLEVQSEDRTPQKWCVSLREDKFEAFLSQGNPTVNKVFGDGSLFSPFLFRKFFDPSDAFPLWEFESDILLSHLRSSGQTTVDWLQTDKDYVLKAELPGVGKNSVQVYVESGKVVEISGLWRHQKEPKTKEWRSGHWWEHGYVRRLELPENADWRRIEAIVNDEIYLEIRIPKCDIPHGKEEGAEDSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YLL9</accession>
    <name>A0AA38YLL9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cytochrome P450 CYP72A219-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_026072</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YLL9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20642">
        <property type="entry name" value="CYP72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000029">
        <property type="entry name" value="Cytochrome P450 734A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050665">
        <property type="entry name" value="Cytochrome_P450_Monooxygen"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24282:SF255">
        <property type="entry name" value="CYTOCHROME P450 72A11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24282">
        <property type="entry name" value="CYTOCHROME P450 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11 12" id="KW-0349">Heme</keyword>
    <keyword evidence="8 11 12" id="KW-0408">Iron</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 12" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="11">
        <location>
            <position position="463"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9672708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="514" mass="58919" checksum="F443A2E148AC5CCD" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YMI4</accession>
    <name>A0AA38YMI4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_026428</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9673142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YMI4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05574">
        <property type="entry name" value="STKc_phototropin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000333">
        <property type="entry name" value="Serine/threonine-protein kinase AGC1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000295">
        <property type="entry name" value="Serine/threonine-protein kinase AGC1-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000028">
        <property type="entry name" value="serine/threonine-protein kinase D6PK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45637">
        <property type="entry name" value="FLIPPASE KINASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="142"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="499"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="43"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="67"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="99"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="506"/>
            <end position="528"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9673142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="540" mass="58992" checksum="78DB49E72F39CCA1" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YN49</accession>
    <name>A0AA38YN49_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glutathione S-transferase TCHQD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_023223</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YN49"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00570">
        <property type="entry name" value="GST_N_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044617">
        <property type="entry name" value="TCHQD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45374">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE TCHQD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45374:SF1">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE TCHQD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13410">
        <property type="entry name" value="GST_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13409">
        <property type="entry name" value="GST_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="2">
        <location>
            <begin position="113"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9673530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="267" mass="31640" checksum="22EFC67FAADF9BC3" modified="2024-01-24" version="1">MQLYHHPYSLDSQKVRLTLEENGIDYTSYHVNPITGKNMEPSFFRLNPSAKLPVFQNGSHIIFDTIDIMQYIERIAVFTLGSENVTLSSQEVIEWMQRIQAWDPKYFTLFHIPEKYRFIVSKFIRRVVIARMAESPDLAGSYHLKLDEVYETEDKLRDPDVLKQSKEQLAEILDDVERKLNESTYLAGDEFTMADVMLIPVLARIVLLDLEEEYICSRPNIAEYWNLVRQRPSYKKVIGKYFSGWRKYKTLLKTWCFVRIRGMLKRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YP65</accession>
    <name>A0AA38YP65_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_023477</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YP65"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10747">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.20:FF:000005">
        <property type="entry name" value="Chaperone protein dnaJ 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.230.10">
        <property type="entry name" value="Heat shock protein DnaJ, cysteine-rich domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.260.20">
        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01152">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012724">
        <property type="entry name" value="DnaJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43096:SF36">
        <property type="entry name" value="CHAPERONE PROTEIN DNAJ 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43096">
        <property type="entry name" value="DNAJ HOMOLOG 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="DnaJ/Hsp40 cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="HSP40/DnaJ peptide-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="J" evidence="5">
        <location>
            <begin position="76"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9673949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="439" mass="49021" checksum="F3E24A0A88B2B5B9" modified="2024-01-24" version="1">MGRFGWLGLCKRHLQSTSAIDFLQIEHCRVPALIHQRILHGYSVLHGKPSDFATMGIPSAMRYIHATGICYSTERNYYEILGVPQNASRDEIKKAFHVLAKKFHPDANKNNPYARRKFQEIRDAYETLQDSEKRAQYDRECTMSSESVKDAAGDGFRYAHRSHFSDSFRKIFAEIFEHETENFATDIQVELSLSFSEAAKGCTKHLSFDAYIPCDSCYGRGHPVDAKTKVCSICKGIGREYCTTCRGSGVVEGVKDVEVVIPAGVDSGDTIHVPKVGNAGGRGVEPGNLYIKLKVAKDHTFVRDGADVYVESSISFTQAILGGTVEVPTLSGKMQLKIPKGVQPGQLLVLRGKGLPKHGLLVDHGDQYVRFRIHFPTAINERQRAILEEFAKEEIVHDSSTSGEGNWWQQIFERGTGPRFMLEMSLFILVLLLLSKTMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YQF1</accession>
    <name>A0AA38YQF1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF4408 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_023974</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YQF1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33640">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33640:SF8">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="66"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="198"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="201"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9674763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="270" mass="31194" checksum="66809110F8D9B6B3" modified="2024-01-24" version="1">MDSIDFDSVKLEKANAMRRYHTLRKFANFLRVLEVSVALFIMLFWSSKRVSNAVKIFSEYLRELCVVLANPRFVFLIGNAIIITLFVKSGRFSGENPYRGNAEPDLYDEYVQHSENSQKVVSQIDRAVTKVSHDDDFVTHSESQQKVLSEIDSPVPASSDDKQIVSWENALHTVLPDPVTTVTKKVPETKFYRRTKSATIRSEKPSRELRRSETDKFQKMDSSGGKSARRLSRVDELSNEEFRRKVEAFIEKQQRLLREEHMAIVLSKQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YTH0</accession>
    <name>A0AA38YTH0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Disease resistance R13L4/SHOC-2-like LRR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_024965</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9676227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YTH0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017:SF570">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN (TIR-NBS CLASS)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="2">
        <location>
            <begin position="148"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9676227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="332" mass="37634" checksum="DC580FF2D4F62847" modified="2024-01-24" version="1">MSRLRLFKVYNTLIWINHLKVDFEFPSSQLRYLHFYGFSLESLPKNFKGRNLVELDLIYSSIKQLWKGNEILDNLKVINLSYSQDLVEIPNFSSVPNLEILNLEGLRYLNLAYYNNLVSFPVTICRLKSLVFLSCSGCSQLNSFPEILENMENLRQLSLDGTAIKELPASIERLGGLQDLHLSNCSNLVNLPESICNLRYLKNLNVNLCLKLEKFPQNLGSLQCLELLGAAGLDSNRVLGAIQSDDCHMSSWEALNRSIDHFSSIIPIIIIQLSKLRVLDLSHCQKLLQIPELPPSIRILDVHACPYLETLSSPSSLLGFSLFKCFKSAIEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YTL6</accession>
    <name>A0AA38YTL6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_021813</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9676461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YTL6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000348">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000090">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF436">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN ELI1, CHLOROPLASTIC-LIKE ISOFORM X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="194"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="327"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9676461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="508" mass="55801" checksum="BDA79812367E6A52" modified="2024-01-24" version="1">MRKEDGNDGNTIVNTSEAINAIRQCKSLKQLHQVHAHTITTGLLWGLHHSSLLLTNILTAFTALLPTANASPTTPAALTGYALSLFNCIPNPSTFGYNTMIRAHTLLSSPLAALVIFVRMRRLSVPPDTHTFPFCLKACSLLPALSLAQTLHSQAFKFGFGADLFVCNTLIHVYSVNKHLHHACQVFNETSLKDVVSYNALIGGFIKVGDTDRARQLFDEMPIRDAVSWGTLLAGYAQSGDLCMDAIQLFNRMLISTVRPDNIALVSALSACAQLGDLEQGKSIHVYIKQNRIPINAFLSTSLVDLYAKCGCIETAREIFESSPDKNLFTWNALLVGLGMHGRGHLSLHYFSRMIEAGIKPDGVSFLGILVGCGHAGLVCEARKFFQEMEVVYRVPRELKHYGCMADLLGRAGLIREAMEMIERMPMGGDVFVWGGVLGGCRIHGNVEIAEKAAENVMVLNPEDDGVYSIMADIYANAGRWEDVARIRRLMHSRQVKKNPGCSLVQLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YU26</accession>
    <name>A0AA38YU26_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cytochrome P450 94B3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_021920</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9676639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YU26"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11064">
        <property type="entry name" value="CYP86A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000044">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24296">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12" id="KW-0349">Heme</keyword>
    <keyword evidence="9 12" id="KW-0408">Iron</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="binding site" description="axial binding residue" evidence="12">
        <location>
            <position position="456"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR602403-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9676639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="514" mass="58168" checksum="E4D5E345E384B8E4" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YXD6</accession>
    <name>A0AA38YXD6_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_022936</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9678194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YXD6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35046:SF26">
        <property type="entry name" value="RNA-DIRECTED DNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35046">
        <property type="entry name" value="ZINC KNUCKLE (CCHC-TYPE) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9678194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="248" mass="28761" checksum="DD795B0F5566114B" modified="2024-01-24" version="1">MVEGYNFFMQPLVNISRFNEEEDWQRTSIFQTCVACQGRLCTLIIDGGSCSNLASEELVEKLNLMLEDHPNPYQIAWVNDTSILVSSRCLVTFNFSNNFELSAWCDVLSMKILRPMKEIPPLKQPEEKLTPLKLEEPSNTPIKKQVKVTSKEEEIINDESTTQEVMELILDQPVEELKEVVEASKELMLDFSRPNIIMSSGKHEESAEISFEHREFKNLILLQDSLQEKYEKQLSTNLLKTHNYFENY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YXE6</accession>
    <name>A0AA38YXE6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Aspartic peptidase DDI1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_023016</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9678282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YXE6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33067:SF32">
        <property type="entry name" value="ASPARTIC PEPTIDASE DDI1-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33067">
        <property type="entry name" value="RNA-DIRECTED DNA POLYMERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13650">
        <property type="entry name" value="Asp_protease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9678282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="271" mass="30394" checksum="E5CC0444C03340E6" modified="2024-01-24" version="1">MKYKDPGCPTISVMIGGTLVEKALLDLGASVNLLPCSVYKQLGLGELEPTSITLSLADRLVKTPRGMIEDVLVQVDNFYYPMDFVVLDTDPIVKGTNCVPIILGRPFLTTSNAIINCRNGLMQLTFGNMTLELNIFNLCNRQFNPKEEEGPEEVCIIDMLVEKHCDQKMQDELNESLGDLDEGLPKPSNLLATLPHWRKIEEILPLFNKEETQEVVKEEAPKLILKPLPTELKYAYLEENKKCPVVISSSLTTDQEECLLEVLRRCKKAIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YYG9</accession>
    <name>A0AA38YYG9_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_020922</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YYG9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9678872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="95" mass="10920" checksum="591A1393FA1B6DB0" modified="2024-01-24" version="1">MSMAIVVMRMKAICVPFSGYGGVHRHDPRDLDGCNRHHRRHHELLHQVHDVPRYDRCRAHDGRDALLRDGHDVLHLHGSQEHVEEEHGEGLGEVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YYN4</accession>
    <name>A0AA38YYN4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">LOB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_021156</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LOB domain-containing protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YYN4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004883">
        <property type="entry name" value="LOB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31301:SF68">
        <property type="entry name" value="LOB DOMAIN-CONTAINING PROTEIN 32-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31301">
        <property type="entry name" value="LOB DOMAIN-CONTAINING PROTEIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03195">
        <property type="entry name" value="LOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50891">
        <property type="entry name" value="LOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LOB" evidence="2">
        <location>
            <begin position="6"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9679140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="262" mass="28869" checksum="62FB5B66E68145BA" modified="2024-01-24" version="1">MSSSNSPCAACKLQRRKCTQECIYAPYFPPDQPQKFANVHKVFGASNVAKLLNEINAANREDAVNSLAYEAEARLRDPVYGCVGMISILQQKLKHLQSDLLAAKKELAVYIGPSAMAHVGYMPQQYMSDPSSSSVFPYNMSPIFGLLPGAPHGGQLIIREPQQQQTQQNQHQRQQILEAQQLAAAMAAGGQEIMRTHEQQQQEQELLRFDNGFDADGIVGAASSFNQMTTAAMSPSQALGAFEHQLQLQPQPQMLPQTQKQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YYU6</accession>
    <name>A0AA38YYU6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TraB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_021229</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YYU6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14726">
        <property type="entry name" value="TraB_PrgY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002816">
        <property type="entry name" value="TraB/PrgY/GumN_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046345">
        <property type="entry name" value="TraB_PrgY-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21530">
        <property type="entry name" value="PHEROMONE SHUTDOWN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21530:SF0">
        <property type="entry name" value="TRAB FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01963">
        <property type="entry name" value="TraB_PrgY_gumN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9679231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="262" mass="29016" checksum="309AE0818EEF11BE" modified="2024-01-24" version="1">MWWWSSVEAEFIFRAGIMYTSNHGELNQQLRSSMFSLSGTGFFGAIGRSINLGGQTALALRLLLAVFSSKISSDVNRPFGDEFRAARKASEKVGAQIVLGDRPIEITLERAWNSLKWNEKLSLVISVIRGITSSELSTNEMKGLSTDDSTFQLYDRLSVAYPSLLQPLIHERDTYLAWSLKRSKAVNGSKRVVGVIGKGHMNGVIYALVSDQGNLRFQDLVGKRSSGDGANGWVDSLLKNLVRDTVIGILLWALFEQIKTVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z023</accession>
    <name>A0AA38Z023_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DYW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_021630</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. PCMP-H subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z023"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000366">
        <property type="entry name" value="Pentatricopeptide (PPR) repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000798">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At3g49170, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001086">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At4g33170"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032867">
        <property type="entry name" value="DYW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF522">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14432">
        <property type="entry name" value="DYW_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="109"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="141"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="244"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="348"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="415"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="474"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="505"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="540"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="domain" description="DYW" evidence="5">
        <location>
            <begin position="715"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF14432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9679775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="807" mass="90156" checksum="A28C191D325772E9" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z063</accession>
    <name>A0AA38Z063_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_021734</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z063"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37258">
        <property type="entry name" value="FANTOM PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37258:SF1">
        <property type="entry name" value="FANTOM PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="209"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="209"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="255"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="266"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="282"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="319"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9679921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="329" mass="36707" checksum="E1F5379D94573CCB" modified="2024-01-24" version="1">MLCSISTGKSGSKWLDRLRSAKGFPTGNDDDLEHFLTHRDPNLSNSPIAKPSDPKSISDSTCSDKKPVQDRSPPPETGEKEWFGIMSNVLAELFNMGDSNQIPKLSGKKSSRKQTNPKICLLSSVRREDDVPATAPSSGDNSLTEMKDSNGEVKTVNQGKVDCLDAEEEKCNQDLSAYSRSEVTVIDTSCAVWKFEKLLFRKKNVWKVRDKKGKSRSIGRKKRKASECDEQLDARKKMKLSVESFKERNEEESAMPSNEEQNPHNAKKEECKETSDGLSQVPKKRSPFSRLPRKSREGGSSVILVRGSKKTGANLPKITLKDTSRRQKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z342</accession>
    <name>A0AA38Z342_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_020393</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9681501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z342"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005247">
        <property type="term" value="F:voltage-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042548">
        <property type="term" value="P:regulation of photosynthesis, light reaction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024701">
        <property type="entry name" value="VCCN1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044669">
        <property type="entry name" value="YneE/VCCN1/2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33281">
        <property type="entry name" value="UPF0187 PROTEIN YNEE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33281:SF1">
        <property type="entry name" value="VOLTAGE-DEPENDENT CHLORIDE CHANNEL 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25539">
        <property type="entry name" value="Bestrophin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016988">
        <property type="entry name" value="UCP016988"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="114"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="155"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="398"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="400"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9681501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="419" mass="47191" checksum="833E570CC0515B39" modified="2024-01-24" version="1">MRKPTHSIQLPTNLAPYSSLKTIPNFHFLPFPSKPTKLSFRVFSSHSADSPPPPSSTQNLTLISILRTVPDWADAIKERGMQQKRSLYNHETWVEHRSSRRHVRHLLSSFSSRVILSLIPPVIAFTSVAVIVASYNSAVTFHWLPEFFPLLRASSLPYQLTAPALALLLVFRTEASYSRFEEGRKAWTKIIAGTNDFARQVIAGVESSGDALLKKALLQYIMAFPVALKCHVIYGSDIRQDLQNLLEVDDLAVVLSSKHHPRCIIEFISQSLQLLNLDDAKRHVLESKLSCFHEGISVCEQLMGIPIPLSYTRLTSRFLVLWHLTLPIILWEDCNWIVVPATFISAASLFCIEEVGVLIEEPFPMLALDELCKLIHNNIQEAIASEKLIQAQLIAKRKNHADEHSPNGRPSSEVDRKLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z3G2</accession>
    <name>A0AA38Z3G2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Water stress and hypersensitive response domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_020325</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LEA type 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9681387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z3G2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009269">
        <property type="term" value="P:response to desiccation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1820:FF:000001">
        <property type="entry name" value="Desiccation protectant protein Lea14-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045043">
        <property type="entry name" value="Lea14-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004864">
        <property type="entry name" value="LEA_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013990">
        <property type="entry name" value="WHy-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31459">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31459:SF19">
        <property type="entry name" value="DESICCATION-RELATED PROTEIN LEA14-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03168">
        <property type="entry name" value="LEA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00769">
        <property type="entry name" value="WHy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117070">
        <property type="entry name" value="LEA14-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Water stress and hypersensitive response" evidence="2">
        <location>
            <begin position="24"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9681387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="151" mass="16566" checksum="6532BEEFA1168271" modified="2024-01-24" version="1">MSQLLDKAKNFVAEKVANIKKPEANLDDVDFKDVSREGVTYIAKVSVSNPYGTAIPICEISYTFKSADRVIASGKMEDPGSLPGNEKTVLEVPVKVPHNILVSLVRDIGADWDIDYQLDLGLTIDLPLFGDFTIPLSRKGEIKLPTLSDVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z3G6</accession>
    <name>A0AA38Z3G6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ripening-related protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_020400</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the kiwellin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9681512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z3G6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd22270">
        <property type="entry name" value="DPBB_kiwellin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039271">
        <property type="entry name" value="Kiwellin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33191:SF58">
        <property type="entry name" value="RIPENING-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33191">
        <property type="entry name" value="RIPENING-RELATED PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24300">
        <property type="entry name" value="KWL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9681512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="154" mass="16732" checksum="43DD20403A70C5D2" modified="2024-01-24" version="1">MLKLRLAGQAARLGVRNHLLEDATKRMTDCCVQGKLYTTYKYSPAVSSHTKAVLTLNSFEKGGDGGAPSEFVALSTGWYNKGSRCLNNITISARGRSMVAMVVDECDSTMGCDEDHDYQPPCPNNIVDASKAVWEALGVPLADWGELDIIWSDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z5J0</accession>
    <name>A0AA38Z5J0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">UDP-glycosyltransferase 89A2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_018671</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z5J0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035251">
        <property type="term" value="F:UDP-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000064">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000143">
        <property type="entry name" value="UDP-glycosyltransferase 89B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48047:SF28">
        <property type="entry name" value="F11M15.8 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48047">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9682791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="485" mass="52650" checksum="2670956C29C14E7D" modified="2024-01-24" version="1">MQGQGKATGGIHTSKVMSGSAQTPVHILMFPYAAQGHMLPLLDLAHQLLLTHPNLTLTLVVTPKNLPFLNPLLSAHPTCVKTLVLEFPHHPSLPPGVENVKDIGIHGNVPIINALGKLHNPIFHWFNSHASPPVAIISDFFLGWTHHLAHQLRIPRITFYSSGAFLSCVSDHLWLNADTALSLPVVSFPQLPNTPSFRAEHLPSICRFYRGSDPDWAFVRDCMTANTLSWGRVFNTFDALEGQYLDHLRTQMGHHRVWSVGPLNLPSGSGSMDRGNPSAESAAFDAVMGWLDGCPDGSVVYVCFGSQKLLKPNQVEALASGLEGSGGRFIWVMRAGSSPPDGFEERVGERGKVIKGWAPQVSILSHRAVGGFLSHCGWNSLIEGVVCGAMILGWPMEADQYVNAMRLVDHLGAAVRVCEGSEAVPDSAELGRKIAEAMSEDSPQKRRAKELRDKALGAVLPGGTSSRDLDALVQELVQLTLKQQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z630</accession>
    <name>A0AA38Z630_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PVL29_018801</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z630"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000173">
        <property type="entry name" value="proline-rich receptor-like protein kinase PERK8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000212">
        <property type="entry name" value="Proline-rich receptor-like protein kinase PERK8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047117">
        <property type="entry name" value="PERK1-13-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47982:SF32">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47982">
        <property type="entry name" value="PROLINE-RICH RECEPTOR-LIKE PROTEIN KINASE PERK4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="271"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="373"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="50"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="163"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="182"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="228"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="401"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ9682954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="709" mass="74416" checksum="3491086A3CE0B94C" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z9M4</accession>
    <name>A0AA38Z9M4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_017127</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C48 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z9M4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.395.10">
        <property type="entry name" value="Adenoviral Proteinase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="4">
        <location>
            <begin position="24"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9684975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="103" mass="12282" checksum="67204FE4DE27BF41" modified="2024-01-24" version="1">MSIFIILYQVKVSVLSIVIVIQEKRPLMDMSEFKFVVLEVVQQLNRTDCDIFVIKFIELWSNGRLSRTIANDKVTKYREKLLTQLIMLPQNEVRENVYQFMDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZAW4</accession>
    <name>A0AA38ZAW4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Retrotransposon Copia-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_017518</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZAW4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9685504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="95" mass="10436" checksum="649E44F63CA1A900" modified="2024-01-24" version="1">MADEFSSSSANLVVLTTGNNLSSQPNTLISINVATQMPFKLNKMNYASWRAQFTNLQFGYDLLGFLNGKTTCPPETILQSGSTTPISNPECKLWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZBZ6</accession>
    <name>A0AA38ZBZ6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">PVL29_015114</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="22">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="18">Plastid</location>
            <location evidence="18">Chloroplast thylakoid membrane</location>
            <topology evidence="18">Single-pass membrane protein</topology>
            <orientation evidence="18">Stromal side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the peptidase M41 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZBZ6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010304">
        <property type="term" value="P:PSII associated light-harvesting complex II catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19501">
        <property type="entry name" value="RecA-like_FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000001">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000001">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.760:FF:000035">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FTSH 6 chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.720.210:FF:000002">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FTSH chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.720.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.760">
        <property type="entry name" value="Peptidase M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01458">
        <property type="entry name" value="FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005936">
        <property type="entry name" value="FtsH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011546">
        <property type="entry name" value="Pept_M41_FtsH_extracell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000642">
        <property type="entry name" value="Peptidase_M41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037219">
        <property type="entry name" value="Peptidase_M41-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01241">
        <property type="entry name" value="FtsH_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23076:SF139">
        <property type="entry name" value="ATP-DEPENDENT ZINC METALLOPROTEASE FTSH 2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23076">
        <property type="entry name" value="METALLOPROTEASE M41 FTSH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06480">
        <property type="entry name" value="FtsH_ext"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01434">
        <property type="entry name" value="Peptidase_M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140990">
        <property type="entry name" value="FtsH protease domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="17 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="9 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0934">Plastid</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0793">Thylakoid</keyword>
    <keyword evidence="13" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="7 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="173"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="21">
        <location>
            <begin position="264"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00060455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAJ9686074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="694" mass="74296" checksum="94ADE19B6FD440C4" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZCR1</accession>
    <name>A0AA38ZCR1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">O-methyltransferase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_015474</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZCR1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016461">
        <property type="entry name" value="COMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001077">
        <property type="entry name" value="COMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11746">
        <property type="entry name" value="O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00891">
        <property type="entry name" value="Methyltransf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51683">
        <property type="entry name" value="SAM_OMT_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="O-methyltransferase C-terminal" evidence="4">
        <location>
            <begin position="12"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9686616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="231" mass="25977" checksum="7612605F4B931F33" modified="2024-01-24" version="1">MVLMAYIARLHEGGIAFEKAHGRQIWNFALENPVFNNLFNDGMACTAKITMKAVVAAYKDGFGCIGTLVDVEGGTGGTGEVVAELVKAYPHIKGINFDLPCVLTSAPAYERVSHVEGDMFESIPKADAIFMKWILHDWNDEDCFKILKNCRKAVLEKTWKIIIVDGVIREDGDDPFDETRLVFDLVMVAHSFNGKERTEVEWKKLLEEGGFTRYRILKIATLQVIIEAYPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZCS8</accession>
    <name>A0AA38ZCS8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_015489</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZCS8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000062">
        <property type="entry name" value="protein STRUBBELIG-RECEPTOR FAMILY 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000095">
        <property type="entry name" value="protein STRUBBELIG-RECEPTOR FAMILY 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000125">
        <property type="entry name" value="Protein STRUBBELIG-RECEPTOR FAMILY 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046959">
        <property type="entry name" value="PRK1-6/SRF4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48007">
        <property type="entry name" value="LEUCINE-RICH REPEAT RECEPTOR-LIKE PROTEIN KINASE PXC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48007:SF68">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="312"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="508"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="251"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="432"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="259"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="436"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9686641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="796" mass="86236" checksum="5A87ED121486C918" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZDQ1</accession>
    <name>A0AA38ZDQ1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_015739</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZDQ1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="2">
        <location>
            <begin position="39"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="159"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="167"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9687002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="231" mass="25825" checksum="61CEEC5C76E4D709" modified="2024-01-24" version="1">MFVGYLIYLDEYSCFHLSLLGGHSSSGSGKGRSHQRPVKYGFRLVKGKANHPMEDYHVAKFVQQQGHELGLFAIYDGHLGDSVPAYLQKHLFPNILKEEEFWTDPVRSISKAYERTDQAILSHSPDLGRGGSTAVTAILIDGRKLWVANVGDSRAVLSKRGQARQMSTDHEPNTERDSIEDRGGFVLSNEEAIDIARKTRDPQRAAKQLVAEALNRESKDDISCIVVRFRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZG31</accession>
    <name>A0AA38ZG31_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_013948</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9687972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZG31"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000015">
        <property type="entry name" value="Transcription factor RAX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000302">
        <property type="entry name" value="Transcription factor TT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015495">
        <property type="entry name" value="Myb_TF_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47999">
        <property type="entry name" value="TRANSCRIPTION FACTOR MYB8-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47999:SF96">
        <property type="entry name" value="TRANSCRIPTION REPRESSOR MYB6-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="10">
        <location>
            <begin position="9"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="9">
        <location>
            <begin position="9"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="10">
        <location>
            <begin position="62"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="9">
        <location>
            <begin position="62"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="116"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="116"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9687972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="284" mass="31744" checksum="65F38E37F4A8DA40" modified="2024-01-24" version="1">MGRTPCCAKEGLNRGSWSAWEDKILSNYVEVHGEGKWRDLPQRAGLKRCGKSCRLRWLNYLRPDIKRGNISSEEEELIIRLHKLLGNRWSLIAGRLPGRTDNEIKNYWNTNLSKRLQASKGQNSPNKKVENPKNQTSGTGKSSAEPHTVIRTKAVRLSKVIIPRVQADFDENPTPKMAVPTSEPSSSALEQGETANFFMGFDIGDLLTSDALNSFLDQDEEMGENNSNGVSDHFPPCSDFFAPEIENQEGVSGLLQPSEALELKTLASFLNSDDEWITENNQVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZH58</accession>
    <name>A0AA38ZH58_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">dolichyl-diphosphooligosaccharide--protein glycotransferase</fullName>
            <ecNumber evidence="7">2.4.99.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">PVL29_014166</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="23">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a di-trans,poly-cis-dolichyl diphosphooligosaccharide + L-asparaginyl-[protein] = N(4)-(oligosaccharide-(1-&gt;4)-N-acetyl-beta-D-glucosaminyl-(1-&gt;4)-N-acetyl-beta-D-glucosaminyl)-L-asparaginyl-[protein] + a di-trans,poly-cis-dolichyl diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22980"/>
            <dbReference type="Rhea" id="RHEA-COMP:12804"/>
            <dbReference type="Rhea" id="RHEA-COMP:12805"/>
            <dbReference type="Rhea" id="RHEA-COMP:19506"/>
            <dbReference type="Rhea" id="RHEA-COMP:19509"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:50347"/>
            <dbReference type="ChEBI" id="CHEBI:57497"/>
            <dbReference type="ChEBI" id="CHEBI:57570"/>
            <dbReference type="ChEBI" id="CHEBI:132529"/>
            <dbReference type="EC" id="2.4.99.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the oligosaccharyltransferase (OST) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the STT3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.99.18" evidence="7"/>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZH58"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004579">
        <property type="term" value="F:dolichyl-diphosphooligosaccharide-protein glycotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12610:FF:000002">
        <property type="entry name" value="dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003674">
        <property type="entry name" value="Oligo_trans_STT3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048999">
        <property type="entry name" value="STT3-PglB_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048307">
        <property type="entry name" value="STT3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13872">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13872:SF48">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT STT3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02516">
        <property type="entry name" value="STT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21436">
        <property type="entry name" value="STT3-PglB_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="17" id="KW-0464">Manganese</keyword>
    <keyword evidence="15 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="10 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="117"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="142"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="172"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="203"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="239"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="270"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="300"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="365"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="392"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="506"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="domain" description="Oligosaccharyl transferase STT3 N-terminal" evidence="21">
        <location>
            <begin position="19"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="STT3/PglB/AglB core" evidence="22">
        <location>
            <begin position="575"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="451"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="748"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="451"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="466"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="19">
        <location>
            <begin position="748"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="19">
        <location>
            <begin position="768"/>
            <end position="778"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF02516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF21436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAJ9688333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="778" mass="86679" checksum="EA8260C4841F41A6" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZHA0</accession>
    <name>A0AA38ZHA0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">AUGMIN subunit 8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_014605</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the QWRF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9689043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZHA0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005880">
        <property type="term" value="C:nuclear microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051225">
        <property type="term" value="P:spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007573">
        <property type="entry name" value="QWRF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31807">
        <property type="entry name" value="AUGMIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31807:SF37">
        <property type="entry name" value="HAUS AUGMIN-LIKE COMPLEX SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04484">
        <property type="entry name" value="QWRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="24"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="146"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="267"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="211"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="273"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="320"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="342"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="360"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9689043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="635" mass="69718" checksum="3409C989F3D38AAC" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZI09</accession>
    <name>A0AA38ZI09_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Serine carboxypeptidase-like 18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_014728</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9689210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZI09"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019748">
        <property type="term" value="P:secondary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12670:FF:000002">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000072">
        <property type="entry name" value="Serine carboxypeptidase-like 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12670">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF487">
        <property type="entry name" value="SERINE CARBOXYPEPTIDASE-LIKE 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041273434" description="Serine carboxypeptidase-like 18" evidence="2">
        <location>
            <begin position="25"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9689210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="459" mass="51102" checksum="7CDC463F35BF0D56" modified="2024-01-24" version="1">MVSMWMGRHLFLTILLFASKAVTSQTIVTSLPGFSGTLPFTLETGYVGVGESEEVQLFYYFVESQSSPSQDPLMLYIAGGPGCSSLGSLLYENGPIYLNYQHYDGGIPSLNLSADAWTQGLNMIYIDAPVGTGFSYSNTSQGYYVDDAENAAQTYEFLRKWLVQHPDFLTNELYIAGVSYSGIPVPMIVNEIIEGNILGLSPSMNIKGYVLGSPVTDSYIDDNSRIPFAYGLTLISHELYNSAKTNCKGNYVNVSSEACALDIEAIDELLRYINVAQVLHPYCYPFTVKPSERQGNRRSSLEEANYRLCDDYSSVPMGIWANDESVRAALNVRNGTKGNWQFCNSSLTGYTEDVTTTVAYHRNLSHTTSLRALIYSGDHDMSIPNIGTQEWIGSLNMTLADTWRAWMVDAQVAGYTKRYTYGDFSLTYATVKGAGHIPATYKTRQCYEMIERWLAHYPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZIV5</accession>
    <name>A0AA38ZIV5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_014625</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9689069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZIV5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19446">
        <property type="entry name" value="REVERSE TRANSCRIPTASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="484"/>
            <end position="783"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9689069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1159" mass="132418" checksum="F689DAF237E1BF91" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZK17</accession>
    <name>A0AA38ZK17_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_012617</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZK17"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000294">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At1g09900"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45613:SF354">
        <property type="entry name" value="OS10G0368902 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45613">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12854">
        <property type="entry name" value="PPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="14"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="216"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="251"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="286"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="321"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="352"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="388"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="423"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="458"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="493"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="528"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="563"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="598"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="633"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="668"/>
            <end position="702"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9690042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="722" mass="81413" checksum="9112996D96F85E9D" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZNZ9</accession>
    <name>A0AA38ZNZ9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_011619</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZNZ9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20653">
        <property type="entry name" value="CYP81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000023">
        <property type="entry name" value="Cytochrome P450 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050651">
        <property type="entry name" value="Plant_Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47947">
        <property type="entry name" value="CYTOCHROME P450 82C3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47947:SF24">
        <property type="entry name" value="ISOFLAVONE 2'-HYDROXYLASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9 10" id="KW-0349">Heme</keyword>
    <keyword evidence="6 9 10" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 9 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 10" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="9">
        <location>
            <position position="446"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9692631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="510" mass="57997" checksum="0A95B42BC22D8B0B" modified="2024-01-24" version="1">METVYMCIPLCLALYLFTRHLLHKLHNLPPTPFLSLPIIGHLYLLKKPLHRTLAGISSRYGPIVFLRLGSRPSLIVSSPSVAEECLTKNDIVFANRPQLIAGKYIGYNYTSLVWANYGDHWRNLRRISTLEILSSSRIQMLSGIRADEVRLLVRWLLENENQTVNMKAMLFEITTNVMMRMIAGKRYYGGSMAEAEETVKFREIMADTIRLGDMSNIGDYLPMLRWLGVKGKEEGLRELQRKRDRFMQSLIEEHRKIMAEDKESSSSCCNGDDGEKKKTMIEVMLSLQEKEPDYYTDLIIRGLMLVLLGAGTDTTATTIEWTLSLLLNNPHALKKAQMEIDNHLGDNHLIQESDLNQLPYLHSIIKESQRMYPVGPIIPHESSGECTVGGYRIPHGTMLLVNIWAIQNDPRVWEEPRKFMPERFEGMELEKHGFRLMPFGSGRRGCPGEGLAVRIVGLVLGSLIQCFDWESVGEGMVDMREGPGLTLPKAQPLLVRCRPRPAFVDLLSKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZSB4</accession>
    <name>A0AA38ZSB4_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_010066</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZSB4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36360">
        <property type="entry name" value="ACTIN T1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36360:SF1">
        <property type="entry name" value="ACTIN T1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9694395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="99" mass="12214" checksum="A2F3702FD115066C" modified="2024-01-24" version="1">MEKYFGNAYRGDPGVPHADPDRFVNIWIGSIAFSALTWVNPYMWQLSNQFNWHDKAMLFEQYHWKKALAKKQPYKFKWNQYMDRDLRDSYYFNWPVYFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZSI1</accession>
    <name>A0AA38ZSI1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">EGF-like calcium-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PVL29_009931</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Cytoplasmic vesicle</location>
            <location evidence="16">Clathrin-coated vesicle membrane</location>
            <topology evidence="16">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="17">Prevacuolar compartment membrane</location>
            <topology evidence="17">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VSR (BP-80) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZSI1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030665">
        <property type="term" value="C:clathrin-coated vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02125">
        <property type="entry name" value="PA_VSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000178">
        <property type="entry name" value="vacuolar-sorting receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000001">
        <property type="entry name" value="Vacuolar-sorting receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056858">
        <property type="entry name" value="VSR_TRX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22702">
        <property type="entry name" value="PROTEASE-ASSOCIATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22702:SF1">
        <property type="entry name" value="PROTEASE-ASSOCIATED DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25011">
        <property type="entry name" value="VSR_TRX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="15" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 19" id="KW-0732">Signal</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041274686" description="EGF-like calcium-binding domain-containing protein" evidence="19">
        <location>
            <begin position="22"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="563"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like calcium-binding" evidence="20">
        <location>
            <begin position="513"/>
            <end position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00029430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00043947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM00179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAJ9694189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="623" mass="69114" checksum="D2355764076EF467" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZSV6</accession>
    <name>A0AA38ZSV6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Transcription factor-like protein DPB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_010057</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Involved in the regulation of the G1/S transition. Increases the DNA binding activity of E2F proteins after heterodimerization.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 11 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11 12">Belongs to the E2F/DP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZSV6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070176">
        <property type="term" value="C:DRM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14458">
        <property type="entry name" value="DP_DD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000187">
        <property type="entry name" value="Transcription factor-like protein DPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.80">
        <property type="entry name" value="Transcription factor DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037241">
        <property type="entry name" value="E2F-DP_heterodim"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003316">
        <property type="entry name" value="E2F_WHTH_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038168">
        <property type="entry name" value="TF_DP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014889">
        <property type="entry name" value="Transc_factor_DP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015648">
        <property type="entry name" value="Transcrpt_fac_DP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12548">
        <property type="entry name" value="TRANSCRIPTION FACTOR DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12548:SF9">
        <property type="entry name" value="TRANSCRIPTION FACTOR DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08781">
        <property type="entry name" value="DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02319">
        <property type="entry name" value="WHD_E2F_TDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009404">
        <property type="entry name" value="Transcription_factor_DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01138">
        <property type="entry name" value="DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01372">
        <property type="entry name" value="E2F_TDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144074">
        <property type="entry name" value="E2F-DP heterodimerization region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 11 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 11 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 11 12" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="E2F/DP family winged-helix DNA-binding" evidence="15">
        <location>
            <begin position="96"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription factor DP C-terminal" evidence="14">
        <location>
            <begin position="186"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="73"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="277"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="26"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="297"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR009404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM01372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9694384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="331" mass="36098" checksum="5CC2E6EC1CA190FF" modified="2024-01-24" version="1">MVTGGSNQEDGEKNPATVSKGGATRSWGTTVSGQSVSTSGSVGSPSSRSEAALATPASENTFLRLNHLDIHGDDAGSQGAANRKKKRGQRAVGGDKSGRGLRQFSMKVCEKVESKGRTTYNEVADELVAEFADPSNSLTSPDQQQYDEKNIRRRVYDALNVLMAMDIISKDKKEIQWKGLPRTSLNDIEELKTDRIGLRGRIEKKAAYLQELEEQFVGLQNLIQRNEQLYSSGNAPSGGVALPFILVQVEFLGNTPFELHDDNYVLKAMKFCERPQGDDVTHDFPADGEGSSIPGMYQSQIPHSSRSSTLGRPPTSPPLPGILKARVKHEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZU16</accession>
    <name>A0AA38ZU16_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Retrovirus-related Pol polyprotein from transposon TNT 1-94</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_010610</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZU16"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025724">
        <property type="entry name" value="GAG-pre-integrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054722">
        <property type="entry name" value="PolX-like_BBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039537">
        <property type="entry name" value="Retrotran_Ty1/copia-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057670">
        <property type="entry name" value="SH3_retrovirus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648">
        <property type="entry name" value="TRANSPOSASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648:SF28">
        <property type="entry name" value="TRANSPOSON-ENCODED PROTEIN WITH RIBONUCLEASE H-LIKE AND RETROVIRUS ZINC FINGER-LIKE DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13976">
        <property type="entry name" value="gag_pre-integrs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22936">
        <property type="entry name" value="Pol_BBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25597">
        <property type="entry name" value="SH3_retrovirus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="7">
        <location>
            <begin position="34"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase catalytic" evidence="8">
        <location>
            <begin position="271"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9695207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1155" mass="131553" checksum="64E0726660992757" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZUK0</accession>
    <name>A0AA38ZUK0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA (cytosine-5-)-methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_010854</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15 16">
            <text>a 2'-deoxycytidine in DNA + S-adenosyl-L-methionine = a 5-methyl-2'-deoxycytidine in DNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13681"/>
            <dbReference type="Rhea" id="RHEA-COMP:11369"/>
            <dbReference type="Rhea" id="RHEA-COMP:11370"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:85452"/>
            <dbReference type="ChEBI" id="CHEBI:85454"/>
            <dbReference type="EC" id="2.1.1.37"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.37" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZUK0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003886">
        <property type="term" value="F:DNA (cytosine-5-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044027">
        <property type="term" value="P:negative regulation of gene expression via chromosomal CpG island methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18635">
        <property type="entry name" value="CD_CMT3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.490:FF:000011">
        <property type="entry name" value="DNA (cytosine-5)-methyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.120.10:FF:000003">
        <property type="entry name" value="DNA (cytosine-5)-methyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.120.10">
        <property type="entry name" value="DNA Methylase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001025">
        <property type="entry name" value="BAH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043151">
        <property type="entry name" value="BAH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050390">
        <property type="entry name" value="C5-Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001525">
        <property type="entry name" value="C5_MeTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023779">
        <property type="entry name" value="Chromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10629">
        <property type="entry name" value="CYTOSINE-SPECIFIC METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10629:SF42">
        <property type="entry name" value="DNA (CYTOSINE-5)-METHYLTRANSFERASE CMT1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01426">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00145">
        <property type="entry name" value="DNA_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00105">
        <property type="entry name" value="C5METTRFRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00439">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51038">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00598">
        <property type="entry name" value="CHROMO_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51679">
        <property type="entry name" value="SAM_MT_C5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="BAH" evidence="12">
        <location>
            <begin position="90"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="Chromo" evidence="11">
        <location>
            <begin position="367"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="339"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="349"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9695580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="829" mass="92529" checksum="6D93A63EBBEB69D1" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZVA6</accession>
    <name>A0AA38ZVA6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_011040</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCA family. CPR flippase (TC 3.A.1.211) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005319">
        <property type="term" value="F:lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03263">
        <property type="entry name" value="ABC_subfamily_A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000904">
        <property type="entry name" value="ABC transporter A family member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000298">
        <property type="entry name" value="ATP-binding cassette sub-family A member 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026082">
        <property type="entry name" value="ABCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056264">
        <property type="entry name" value="R2_ABCA1-4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229:SF267">
        <property type="entry name" value="ABC TRANSPORTER A FAMILY MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER SUBFAMILY A ABCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12698">
        <property type="entry name" value="ABC2_membrane_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23321">
        <property type="entry name" value="R1_ABCA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="284"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="324"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="359"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="387"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="416"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="455"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="983"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1178"/>
            <end position="1201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1235"/>
            <end position="1254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1266"/>
            <end position="1284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1296"/>
            <end position="1315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1327"/>
            <end position="1346"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="552"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="1455"/>
            <end position="1693"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9695867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1881" mass="209328" checksum="6D14EDE3F6B7A8D9" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZWA2</accession>
    <name>A0AA38ZWA2_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_008384</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZWA2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9696110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="147" mass="16264" checksum="49A9A598791ED24F" modified="2024-01-24" version="1">MEAMDKKSDVKPTRSFLGKSMRKSLSWYREVRMARNMITALTEKQSATREDMAKMSNAGLSSRFGVPTFSFTPPGTVADVAKATVAMRAATTEHDSEVLNSHSPPAWTRSVCFSVKISFAVSPFWLQRTKKCCGTDLLTNCIVQSSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZWT0</accession>
    <name>A0AA38ZWT0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_010979</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZWT0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.210:FF:000009">
        <property type="entry name" value="Zinc finger CCCH domain-containing protein 66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045234">
        <property type="entry name" value="Unkempt-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057444">
        <property type="entry name" value="Znf-CCCH_AtC3H23-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14493:SF50">
        <property type="entry name" value="RING FINGER PROTEIN UNKEMPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14493">
        <property type="entry name" value="UNKEMPT FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25512">
        <property type="entry name" value="zf-CCCH_AtC3H23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 7" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="92"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="9">
        <location>
            <begin position="279"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="7">
        <location>
            <begin position="279"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="168"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="646"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="168"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="668"/>
            <end position="677"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9695774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="703" mass="76549" checksum="DF2D0A67670CCE6D" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZXJ6</accession>
    <name>A0AA38ZXJ6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DDE Tnp4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_008397</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HARBI1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZXJ6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035098">
        <property type="term" value="C:ESC/E(Z) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035102">
        <property type="term" value="C:PRC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045249">
        <property type="entry name" value="HARBI1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930:SF85">
        <property type="entry name" value="GH03217P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE Tnp4" evidence="9">
        <location>
            <begin position="196"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9696134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="443" mass="49918" checksum="DF9427B1EB7F657F" modified="2024-01-24" version="1">MAPPKKSKKAKRESKKSKKCKSMSVGPIEPRATESDWWDSFWQKNSPIPVFSATKWRDACNPFKNASSSIPTDEAEGFKFFFRVSKKTFDYICSLVRQDLVSRPPSGLINIEGRLLSVEKQVAIALRRLASGESQVSVGAAFGVGQSTVSQVTWRFIEAVEERAKHHLRWPDFNRMEEIKSKFETLYGLSNCCGAIDASHIIMTLPAVQTSDDWCDQENNYSMFLQGVVDDEMRFLDIVTGWPGGMTVSRLLKCTGFYRNCEAGERLNGNIRILSEGVEIREYVVGGVGYPLLPWLITPCEYNDISSSMSGFKTNHEAARSLAVRAFSQLKGTWRILSKVMWRPDKRKLPSIILVCCLLHNIVIDSKDKLQPDVALSSHHDPGYGQQCCKQVDPLGKAMRENIAKHFCSMAKRRLHQIDQSPSSLPKLNLTVLAQPMFELEPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZXT4</accession>
    <name>A0AA38ZXT4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EID1-like F-box protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_008472</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZXT4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040267">
        <property type="entry name" value="EID1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31348">
        <property type="entry name" value="EID1-LIKE F-BOX PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31348:SF3">
        <property type="entry name" value="EID1-LIKE F-BOX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="248"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="248"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="265"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9696254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="300" mass="32986" checksum="6057EFB99B0174C2" modified="2024-01-24" version="1">MSANQRLRLNSPGHASDSGESGILNERILVLIFESIKWDIHVLCAASAVNRKLRAVATRLLWRELCVYRAPRMVAALATGAPNGRLGGGWHALAKLMFFCCGCESTRHFRVSRPSPGHFVKESRFSKTSGRSFLTKKCRGDLLYVSDPCEHPMGEREDDLGIYRGVFRGFMRSKTRACLIRRQVELEERVCCPYCGARVWSMTAARLVPKSASRRLGTHDGGLEYFVCLNGHLHGTCWLVPLSSDEDGGDAEDGDEGCSDDADDGSCRSECDDRTVTDGSMSSISEEIAADGPPDQSLCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZZN9</accession>
    <name>A0AA38ZZN9_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_009182</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZZN9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="197"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="290"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="210"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="304"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9697273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="318" mass="35569" checksum="186B34BFCE3DDA6F" modified="2024-01-24" version="1">MTTQGAQSPTTIYFNIDGCEGILEARHIAEALHIPFQPEDPSHFRQWSPISQRDMVRILSRGTSGASFILRRELPPGMLLVDVLLRSNLFPLQHLVQRRGSILDALFRISEGFYFGPHHLIMAALLYFEEKVHRKKLQRADTIPLLFPRLLCHILDHLGHPSEPHLERRHHCREHFTLDQWTQLDGSPIDCIPPAPAVPTTPSMPQATPTDPPATPPVPPAAPAPSEAPITIFATEFRAMIHLFKTLTATHNALFQQMRDIRAQQDPHTVIVRQIQQHLGLSPLHTDILGPSEPLVSAEETIPPQATQEAATEPSSPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A0V2</accession>
    <name>A0AA39A0V2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_007142</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9697873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A0V2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000344">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000090">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF453">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT (PPR) SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12854">
        <property type="entry name" value="PPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="180"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="215"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="250"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="351"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="422"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9697873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="555" mass="61738" checksum="73A1F9FD783A7451" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A1C1</accession>
    <name>A0AA39A1C1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Axial regulator YABBY 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_007835</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the YABBY family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A1C1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010158">
        <property type="term" value="P:abaxial cell fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003002">
        <property type="term" value="P:regionalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00084">
        <property type="entry name" value="HMG-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.10:FF:000012">
        <property type="entry name" value="axial regulator YABBY 5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006780">
        <property type="entry name" value="YABBY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056775">
        <property type="entry name" value="YABBY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056776">
        <property type="entry name" value="YABBY_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31675:SF30">
        <property type="entry name" value="AXIAL REGULATOR YABBY 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31675">
        <property type="entry name" value="PROTEIN YABBY 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04690">
        <property type="entry name" value="YABBY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24868">
        <property type="entry name" value="YABBY_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="YABBY N-terminal" evidence="9">
        <location>
            <begin position="6"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="YABBY protein C-terminal" evidence="8">
        <location>
            <begin position="96"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="91"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF24868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9698959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="184" mass="20423" checksum="DC5B41893881E3B2" modified="2024-01-24" version="1">MSLDITPERVCYVHCNFCNTILAVSVPCASLFTIVTVRCGHCANLLSVNMGALLQTVPTQDLQSQKQQLSCGNPSEDCGSSSKCNKFSAFESAEHEQPRMPPIRPAPEKRQRVPSAYNRFIKEEIQRIKASNPDITHREAFSTAAKNWAHFPHIHFGLKLDGNKQGKLDQAFAGEGPHKAHGFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A1U5</accession>
    <name>A0AA39A1U5_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_008087</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9699316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A1U5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012862">
        <property type="entry name" value="DUF1635"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33431">
        <property type="entry name" value="ENABLED-LIKE PROTEIN (DUF1635)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33431:SF3">
        <property type="entry name" value="ENABLED-LIKE PROTEIN (DUF1635)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07795">
        <property type="entry name" value="DUF1635"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="201"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="212"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="230"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9699316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="270" mass="30388" checksum="632FEC292AE407C9" modified="2024-01-24" version="1">MDNQRLTPLLNWAYYCQGKNMEELRHSLVCTTLELETMRLAAQEELRRRDDQLAHLKDLLTRTIRERDEAQDKCHRLILEKILLHQQHQQTANLSGISSIEDEPRRGIDSNNGFSSSDCEESIVSSPVIDPVPPPQLPQQQPPPPPPQLQQPPSMPEAALQLVSDKPLPEKGKLLQAVMKAGPLLQTLLLAGPLPQWRHPPPHLESFEIPPVTIPSQPPPPPQPLLHQDSLLNSSPNNNNINCGRVDRKRALCEDSDTSIETKYQRVALP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A368</accession>
    <name>A0AA39A368_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_008261</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Heterodimer with UBC35 or UBC36.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9699582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A368"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23807">
        <property type="entry name" value="UEV_UBE2V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000019">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 variant 1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBC core" evidence="2">
        <location>
            <begin position="4"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00065118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9699582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="156" mass="17995" checksum="ADB5FFE1D1575947" modified="2024-01-24" version="1">MMFPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNSVHEGRIYQLKLFCDKDYPEKPPSVRFHSRINMTCVNHETGVVEAKKFGMLANWQREFTMEDILTQLKKEMAAPHNRKLVQPPEGTYFYGILCCVLITNGSKSEVKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A4R0</accession>
    <name>A0AA39A4R0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein TIFY</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Jasmonate ZIM domain-containing protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_005976</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Repressor of jasmonate responses.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="2">The jas domain is required for interaction with COI1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the TIFY/JAZ family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A4R0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031347">
        <property type="term" value="P:regulation of defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000022">
        <property type="term" value="P:regulation of jasmonic acid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009611">
        <property type="term" value="P:response to wounding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040390">
        <property type="entry name" value="TIFY/JAZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010399">
        <property type="entry name" value="Tify_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33077:SF42">
        <property type="entry name" value="PROTEIN TIFY 4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33077">
        <property type="entry name" value="PROTEIN TIFY 4A-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06200">
        <property type="entry name" value="tify"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00979">
        <property type="entry name" value="TIFY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51320">
        <property type="entry name" value="TIFY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1184">Jasmonic acid signaling pathway</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tify" evidence="4">
        <location>
            <begin position="150"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="76"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="116"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="294"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="298"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="320"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU369065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9700437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="352" mass="38698" checksum="E9E9085C5A6B72EE" modified="2024-01-24" version="1">MNPGVTTLRSILDKPLQELTEEDISQLTREDCRKYLKEKGMRRPSWNKSQAIQQVISLKSLLETSEGSGAGVLRKITVSSPPENPPPVTSNSADSGKELSADIQISVSADELVPLLPKDHHPESTPSGELASRPPEADTKHTSPRSPGATNCLVGQMTIFYCGKVNVYDGVPDDKAQAIMHLAASPFHLPSDDPFSDAAMLCSSPCHLHTANVKHGHIPPRATVSQIMQTEKFTEYSQQYREEGNFTRGHDVEGQVDRKLSLQRYFEKRRDRFKSRKKIGLPSASLEMYLNHQARTQPSNGQSSRSGTSSPPQHGLLHTLCSSADNHTKNSTPFVDLNSKASILQELPTTPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A684</accession>
    <name>A0AA39A684_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MACPF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_006543</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A684"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009626">
        <property type="term" value="P:plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000031">
        <property type="term" value="P:regulation of salicylic acid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044663">
        <property type="entry name" value="CAD1/NSL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020864">
        <property type="entry name" value="MACPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33199">
        <property type="entry name" value="MACPF DOMAIN-CONTAINING PROTEIN CAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33199:SF2">
        <property type="entry name" value="OS02G0475300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01823">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00457">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51412">
        <property type="entry name" value="MACPF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MACPF" evidence="1">
        <location>
            <begin position="3"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9701240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="614" mass="68549" checksum="BD8D69B2DCE1536A" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A710</accession>
    <name>A0AA39A710_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">thermospermine synthase</fullName>
            <ecNumber evidence="5">2.5.1.79</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_004029</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>S-adenosyl 3-(methylsulfanyl)propylamine + spermidine = thermospermine + S-methyl-5'-thioadenosine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:30515"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:57443"/>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
            <dbReference type="ChEBI" id="CHEBI:59903"/>
            <dbReference type="EC" id="2.5.1.79"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the spermidine/spermine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.79" evidence="5"/>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A710"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010487">
        <property type="term" value="F:thermospermine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006596">
        <property type="term" value="P:polyamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000088">
        <property type="entry name" value="Polyamine aminopropyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.140.10:FF:000006">
        <property type="entry name" value="thermospermine synthase ACAULIS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.140.10">
        <property type="entry name" value="Spermidine synthase, tetramerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00198">
        <property type="entry name" value="Spermidine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030374">
        <property type="entry name" value="PABS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001045">
        <property type="entry name" value="Spermi_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035246">
        <property type="entry name" value="Spermidine_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037163">
        <property type="entry name" value="Spermidine_synt_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037959">
        <property type="entry name" value="MFS_SpdSyn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43317">
        <property type="entry name" value="THERMOSPERMINE SYNTHASE ACAULIS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43317:SF1">
        <property type="entry name" value="THERMOSPERMINE SYNTHASE ACAULIS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17284">
        <property type="entry name" value="Spermine_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01564">
        <property type="entry name" value="Spermine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51006">
        <property type="entry name" value="PABS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PABS" evidence="7">
        <location>
            <begin position="31"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9702092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="336" mass="38040" checksum="88644DFBF2A2CA4A" modified="2024-01-24" version="1">MGEAVEFYHSNGVSGINHKESGKTIDENHMCCWYEEVIDDNLKWSFALNSVLHKGTSQYQDIVLLDTKHFGKALVIDGKMQSAEVDEFIYHECLIHPALLCHPNPKTIFIMGGGEGSAAREALRHNSIEKVVMCDIDQEVVDFCRRYLTVNQEAFRNKKLNLVINDAKAELERRNEKFDIIVGDLADPVEGGPCYQLYTKSFYERILKPKLNVNGIFVTQAGPAGIFTHKEVFSSIYNTVKQVFKYVVAYAAHVPSFADTWGWVMASDQPFCINAEEIDKKIDKRIDGELLYLNGASFISSTTMNKSVSLSLLHETHIYTEEDARFIHGHGVAYRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A7J2</accession>
    <name>A0AA39A7J2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NFACT RNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_004206</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CCDC25 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A7J2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039730">
        <property type="entry name" value="Jlp2/Ccd25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008532">
        <property type="entry name" value="NFACT_RNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13049:SF2">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13049">
        <property type="entry name" value="DUF814-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05670">
        <property type="entry name" value="NFACT-R_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NFACT RNA-binding" evidence="3">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="194"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="194"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9702362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="215" mass="25247" checksum="928C83EEAB52D24E" modified="2024-01-24" version="1">MVFYFKARPEVGDYTIFMGLDKYENEELIKYGFPEDIWFHVDKMSSAHVYVRLNKGQTIDDISEGLLEDCAQLVKANSIQGNKVNNVDVVYTPWHNLKKTASMDVGQVGFYNSKMVRTVRVEKRINEIVNRLNRTKVERKPDLKAEREAVNAAERAERKLQLRDKKRREEMERLEKERQAEIRSYKGLMVSEKMTSNKQIASENKSLQELEDDFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A813</accession>
    <name>A0AA39A813_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">3-beta hydroxysteroid dehydrogenase/isomerase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_004065</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A813"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006694">
        <property type="term" value="P:steroid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002225">
        <property type="entry name" value="3Beta_OHSteriod_DH/Estase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF564">
        <property type="entry name" value="STEROL-4-ALPHA-CARBOXYLATE 3-DEHYDROGENASE, DECARBOXYLATING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01073">
        <property type="entry name" value="3Beta_HSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3-beta hydroxysteroid dehydrogenase/isomerase" evidence="3">
        <location>
            <begin position="9"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9702147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="121" mass="13391" checksum="83B97B425C4C77DD" modified="2024-01-24" version="1">MEGEQKWCVVTGGRGFAARHLVEMLIRSGRFSVRIADLGPSIKLEPSEEKGLLGEALQSGRAEYVSADLRYKAQVLKACQGAEVVFHMAAPDSSINNHQLHHSVNVQGKKCWLLRLNLTCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A828</accession>
    <name>A0AA39A828_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein FAR1-RELATED SEQUENCE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_004375</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Putative transcription activator involved in regulating light control of development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the FHY3/FAR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A828"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004330">
        <property type="entry name" value="FAR1_DNA_bnd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031052">
        <property type="entry name" value="FHY3/FAR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018289">
        <property type="entry name" value="MULE_transposase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006564">
        <property type="entry name" value="Znf_PMZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31669:SF88">
        <property type="entry name" value="PROTEIN FAR1-RELATED SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31669">
        <property type="entry name" value="PROTEIN FAR1-RELATED SEQUENCE 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03101">
        <property type="entry name" value="FAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10551">
        <property type="entry name" value="MULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04434">
        <property type="entry name" value="SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00575">
        <property type="entry name" value="ZnF_PMZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="7">
        <location>
            <begin position="594"/>
            <end position="630"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9702601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="710" mass="82752" checksum="1A213D057FAC550D" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A869</accession>
    <name>A0AA39A869_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_004489</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A869"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19446">
        <property type="entry name" value="REVERSE TRANSCRIPTASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="429"/>
            <end position="709"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9702780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="906" mass="103595" checksum="91442F1F9E75BE98" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AA66</accession>
    <name>A0AA39AA66_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rhodanese domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_005150</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AA66"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00158">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044684">
        <property type="entry name" value="STR17/STR18/HARC1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44542">
        <property type="entry name" value="THIOSULFATE SULFURTRANSFERASE 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44542:SF12">
        <property type="entry name" value="THIOSULFATE SULFURTRANSFERASE 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="1">
        <location>
            <begin position="22"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9703741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="129" mass="14005" checksum="B98DF3A078A6C99E" modified="2024-01-24" version="1">MDSQSSATEVVTIDVHAAKDLINSGCRYLDVRTVEEFKKGHPDVENILNIPYLFITPEGRVKNPEFLEQVQSACSKEDHLIVGCQSGVRSLAATSVLVSAGFKDVKDIGGGYLAWVQNGLAATKPKEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AAG3</accession>
    <name>A0AA39AAG3_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_005297</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AAG3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36042">
        <property type="entry name" value="OS05G0490900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36042:SF1">
        <property type="entry name" value="OS05G0490900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="112"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="147"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9703956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="190" mass="20949" checksum="34927F31ED194F3D" modified="2024-01-24" version="1">MAAASLLCLCDTNLIHSSRQESPWHPKLFPGVGHKILDLSVTRKRNRTHTGHQKGNLSHKFSVSATTEGSAKSNKSEETIPSWAQPDSSEPPPWAQDEGMGNASEKSFEIPFYVYLLTSAVTAIAAIGSIFEYANKRPVFGVLSSDSIFYAPLLGFFVFTGIPTSAFLWFKSVQAANKEAEEQDRRDGYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AAK1</accession>
    <name>A0AA39AAK1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Transducin/WD40 repeat-like superfamily protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_005127</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat SWD2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AAK1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000446">
        <property type="entry name" value="protein ANTHESIS POMOTING FACTOR 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037867">
        <property type="entry name" value="Swd2/WDR82"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19861:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 82"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19861">
        <property type="entry name" value="WD40 REPEAT PROTEIN SWD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0806">Transcription termination</keyword>
    <keyword evidence="4 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="179"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="266"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="407"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="25"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9703699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="481" mass="53306" checksum="984E103BF0AC31E0" modified="2024-01-24" version="1">MEKSKGRRRNMMKSHLCIPRLTRGNTSCKSSSSTTNNRLSPAMSLLERFREAVFRLIMLTALSKATSHSGSRDVPRSYYPNEPHHSEAVADCIEFIKKSASPGDNRDSTASSSMDTAMPVPYNTEVIRALPPLCKAVEFESFTTTNQMAGGQGQSEREDKVALELTEEIFQSMEVGMAFRDYSGRISSMDFHRTSSYLVTASDDESIRLYDVATGTCLKTINSKKYGVDLVCFTSDPTTVIYSSKNGWDESLRLLSLHDNKYLRYFKGHHDRVVSLSLCSRKECFISGSLDRTVLLWDQRAEKCQGLLRVQGRPATAYDDQGLVFAIAFGGYVRLFDARKYDKGPFEIFSVGGDISDANVVKFSNDGRLMLLTTMDGHIHVLDSFRGTLLSTYNVKPVSSTSTLEASFSPEGMFVISGSGDGSVYAWSVRSGKEVASWMSTETEPPVIKWAPGSLMFVTGSSELSFWIPDLSKLGAYAGRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ABU6</accession>
    <name>A0AA39ABU6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">CRAL-TRIO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_003003</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the SFH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ABU6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.525.10:FF:000011">
        <property type="entry name" value="SEC14 cytosolic factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.20">
        <property type="entry name" value="N-terminal domain of phosphatidylinositol transfer protein sec14p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051026">
        <property type="entry name" value="PI/PC_transfer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45657">
        <property type="entry name" value="CRAL-TRIO DOMAIN-CONTAINING PROTEIN YKL091C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45657:SF5">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL_PHOSPHATIDYLCHOLINE TRANSFER PROTEIN SFH6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03765">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00180">
        <property type="entry name" value="CRETINALDHBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM01100">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="485"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="domain" description="CRAL-TRIO" evidence="11">
        <location>
            <begin position="157"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="19"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="550"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="22"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9704745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="633" mass="72311" checksum="E7DA51105211EA89" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ACI3</accession>
    <name>A0AA39ACI3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycosyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_003245</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="5"/>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ACI3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035251">
        <property type="term" value="F:UDP-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000047">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000608">
        <property type="entry name" value="Os01g0598000 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48047">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48047:SF81">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9705105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="474" mass="52633" checksum="4F25F7F4DF2D7CDD" modified="2024-01-24" version="1">MASDAGKVEMLFFPYIGGGHLIPMVDLARVFASRGAKSTIITAPDNALLIHKAILRDQKLGHDINLHTLESPSAPISFGDMSAPPFTDTTVLREPLRQLLIQRPPDCVVTDMFHRWVADDVHELGIRIIVFNGSGCFPRCGEDSLRRYSPHEKVGSESEVFVLPGLPDRIELTRSQLPKFDRLPNKPPTMMNWEAKIYGSVVNSFYELEPAYVDYFRNQLGKKAWLVGPVCLCNKNIEDKAGRGQEASIDEQACLNWLDSKQPNSVLYVSFGSLARLPPRQLLEIACALEASGRPFIWVVGKVFQTVAGGEENWLPSGYEERMVISKKGLIIRGWAPQLLILEHAAIGGFVTHCGWNSTLEAVCAGVPMITWPLTAEQFLNEKLVTDVLRVGVKVGSMDWRSWKDEPKEVVGREKIQTAVERVMGGGEEAVEMRSRGREVAEKAKRAVEEGGSSYSDAIAVIEELKACRKDGKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AGD3</accession>
    <name>A0AA39AGD3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_002170</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AGD3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF78">
        <property type="entry name" value="OS12G0587133 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9707057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="614" mass="70597" checksum="47385D4346851DF1" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AHX6</accession>
    <name>A0AA39AHX6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="7">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_001849</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="domain">
        <text evidence="7">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DEAD box helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="7"/>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AHX6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="11">
        <location>
            <begin position="285"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="316"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="533"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="116"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="178"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="6">
        <location>
            <begin position="285"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9706574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="754" mass="85316" checksum="85B34284FCFC8D0B" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AIT4</accession>
    <name>A0AA39AIT4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">cellulase</fullName>
            <ecNumber evidence="4">3.2.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_000190</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endohydrolysis of (1-&gt;4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 9 (cellulase E) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.4" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AIT4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008810">
        <property type="term" value="F:cellulase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000020">
        <property type="entry name" value="Endoglucanase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019028">
        <property type="entry name" value="CBM_49"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001701">
        <property type="entry name" value="Glyco_hydro_9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22298">
        <property type="entry name" value="ENDO-1,4-BETA-GLUCANASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09478">
        <property type="entry name" value="CBM49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00759">
        <property type="entry name" value="Glyco_hydro_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01063">
        <property type="entry name" value="CBM49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041330805" description="cellulase" evidence="11">
        <location>
            <begin position="26"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="domain" description="Carbohydrate binding" evidence="12">
        <location>
            <begin position="503"/>
            <end position="583"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9708004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="595" mass="65939" checksum="307C728EDC829BA0" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AKD8</accession>
    <name>A0AA39AKD8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein DETOXIFICATION</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Multidrug and toxic compound extrusion protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_000701</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AKD8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13132">
        <property type="entry name" value="MATE_eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045069">
        <property type="entry name" value="MATE_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="145"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="179"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="210"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="237"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="283"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="321"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="361"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="402"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="434"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="463"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9708814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="524" mass="57483" checksum="E3A15E330B434272" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AKP6</accession>
    <name>A0AA39AKP6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Amine oxidase</fullName>
            <ecNumber evidence="16">1.4.3.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PVL29_000702</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a primary methyl amine + O2 + H2O = an aldehyde + H2O2 + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:16153"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:228804"/>
            <dbReference type="EC" id="1.4.3.21"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:16154"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>L-topaquinone</name>
            <dbReference type="ChEBI" id="CHEBI:79027"/>
        </cofactor>
        <cofactor evidence="16">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="16">Contains 1 topaquinone per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>L-topaquinone</name>
            <dbReference type="ChEBI" id="CHEBI:79027"/>
        </cofactor>
        <text evidence="15">Contains 1 topaquinone per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="15 16">Topaquinone (TPQ) is generated by copper-dependent autoxidation of a specific tyrosyl residue.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 16">Belongs to the copper/topaquinone oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.-" evidence="16"/>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AKP6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008131">
        <property type="term" value="F:primary methylamine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009308">
        <property type="term" value="P:amine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.98.20:FF:000001">
        <property type="entry name" value="Amine oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.40:FF:000002">
        <property type="entry name" value="Amine oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.40:FF:000004">
        <property type="entry name" value="Amine oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.20">
        <property type="entry name" value="Copper amine oxidase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000269">
        <property type="entry name" value="Cu_amine_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015798">
        <property type="entry name" value="Cu_amine_oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036460">
        <property type="entry name" value="Cu_amine_oxidase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016182">
        <property type="entry name" value="Cu_amine_oxidase_N-reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015800">
        <property type="entry name" value="Cu_amine_oxidase_N2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015802">
        <property type="entry name" value="Cu_amine_oxidase_N3"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008559">
        <property type="entry name" value="PRK11504.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10638:SF80">
        <property type="entry name" value="AMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10638">
        <property type="entry name" value="COPPER AMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01179">
        <property type="entry name" value="Cu_amine_oxid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02727">
        <property type="entry name" value="Cu_amine_oxidN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02728">
        <property type="entry name" value="Cu_amine_oxidN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49998">
        <property type="entry name" value="Amine oxidase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54416">
        <property type="entry name" value="Amine oxidase N-terminal region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 16" id="KW-0186">Copper</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0464">Manganese</keyword>
    <keyword evidence="6 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14 16" id="KW-0801">TPQ</keyword>
    <feature type="domain" description="Copper amine oxidase N2-terminal" evidence="18">
        <location>
            <begin position="82"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="Copper amine oxidase N3-terminal" evidence="19">
        <location>
            <begin position="207"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="Copper amine oxidase catalytic" evidence="17">
        <location>
            <begin position="336"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="414"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate; via topaquinone" evidence="14">
        <location>
            <position position="498"/>
        </location>
    </feature>
    <feature type="modified residue" description="2',4',5'-topaquinone" evidence="15">
        <location>
            <position position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00051381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR600269-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR600269-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAJ9708822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="774" mass="86323" checksum="4EA257C09A6861C2" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AKZ3</accession>
    <name>A0AA39AKZ3_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_000792</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AKZ3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035445">
        <property type="entry name" value="GYF-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46992">
        <property type="entry name" value="GYF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46992:SF4">
        <property type="entry name" value="GYF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55277">
        <property type="entry name" value="GYF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9708972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="69" mass="7699" checksum="FD462C71BC3EABBA" modified="2024-01-24" version="1">MGFRELKDPAGKNHGPFLGVGIISGFKQGFFGMNLLVRLSVAPEGMPFHDLDEEMNPRHEASRLECLKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ALG8</accession>
    <name>A0AA39ALG8_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_001301</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ALG8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36381">
        <property type="entry name" value="ETHYLENE-REGULATED TRANSCRIPT 2 (ERT2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36381:SF1">
        <property type="entry name" value="ETHYLENE-REGULATED TRANSCRIPT 2 (ERT2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="131"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9709772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="208" mass="21949" checksum="85B2967B2D3B6D88" modified="2024-01-24" version="1">MEEHKGGANFAVCGGSPVTQARWVAGRGDRLPHLAHRSLCQELGLPQETQKKAFLCLRSNPAAACGASTASRVCAGSDVFRFFSEIKSVECEEEEIGGIDGGSLVDGGGEVEESVGRPSGRGVTMAVLKMLVVVILALGTKKLAVGITMFAFLLLFLEYAAKWVFGLLTPCSDAQIAIKSLIPKAPIPRTPSNASVEEIQIAESQFVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D3E6</accession>
    <name>A0AA39D3E6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Subtilisin-like protease SBT1.7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PVL29_026831</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the peptidase S8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D3E6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02120">
        <property type="entry name" value="PA_subtilisin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04852">
        <property type="entry name" value="Peptidases_S8_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.2310:FF:000001">
        <property type="entry name" value="Subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000006">
        <property type="entry name" value="Subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000005">
        <property type="entry name" value="subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.80:FF:000003">
        <property type="entry name" value="Subtilisin-like protease SBT1.9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034197">
        <property type="entry name" value="Peptidases_S8_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010259">
        <property type="entry name" value="S8pro/Inhibitor_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045051">
        <property type="entry name" value="SBT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041469">
        <property type="entry name" value="Subtilisin-like_FN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10795">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17766">
        <property type="entry name" value="fn3_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05922">
        <property type="entry name" value="Inhibitor_I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 11" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 11" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041435027" description="Subtilisin-like protease SBT1.7" evidence="13">
        <location>
            <begin position="24"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="domain" description="Inhibitor I9" evidence="16">
        <location>
            <begin position="34"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="14">
        <location>
            <begin position="133"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="15">
        <location>
            <begin position="384"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="Subtilisin-like protease fibronectin type-III" evidence="17">
        <location>
            <begin position="659"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="198"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="200"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="10 11">
        <location>
            <position position="141"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="10 11">
        <location>
            <position position="214"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="10 11">
        <location>
            <position position="544"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF05922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF17766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ9670538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="20">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="767" mass="81470" checksum="7073EE357D48E827" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D5Y4</accession>
    <name>A0AA39D5Y4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Putative plant transposon protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_027256</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9671185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D5Y4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046796">
        <property type="entry name" value="Transposase_32_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20167">
        <property type="entry name" value="Transposase_32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Putative plant transposon protein" evidence="2">
        <location>
            <begin position="67"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="262"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="317"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="269"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="329"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9671185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="353" mass="40108" checksum="FE7F631C1D40084A" modified="2024-01-24" version="1">MPPKKKVRHGSTSTAPEEVPQDPFMPRHPPDEHECDRLIGKRPIAVERVILIAGFEEHGVAELLEFYKLRPFIQLTQECYPTPVRAFYSNIRNVDEAKYSFDTSFKGVAVRVSPSLIGRTFSLETPKKAINFCIPEIDIKGMSDIITKELCGHSFELGTSVERTSLLPKYRILSLILFHTVLNKKGHLNELTLYVLHILFHMARRHKINLPALICHNMIEARRSNVSTRALPYGSTVCLLLQNAGLDFNTLPYESMPRMQPMRMKQSMDKQTAASSSRRPTKSSQLGDIYAMCKSMNNQLKLVRQQCDRSIAAWKTLHLDALPPTPSQTDDEEDGDDDGEDEEEEDAPIDEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D6E5</accession>
    <name>A0AA39D6E5_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_025446</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D6E5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35292">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35292:SF13">
        <property type="entry name" value="OS03G0581800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9671740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="100" mass="11218" checksum="D95B03EB35271A00" modified="2024-01-24" version="1">MAAWTAVARQAWNLTRLSSPRPAPQSTSLIHRRGLASGGDHHGPPKVNFWQDPLSPSKWKEEHFVIVSLSGWGLLFYGGYKYFTKAKKDNKEEKVGDASH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D6G0</accession>
    <name>A0AA39D6G0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">O-fucosyltransferase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_023320</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase GT106 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D6G0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006004">
        <property type="term" value="P:fucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11299">
        <property type="entry name" value="O-FucT_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024709">
        <property type="entry name" value="FucosylTrfase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019378">
        <property type="entry name" value="GDP-Fuc_O-FucTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31288:SF8">
        <property type="entry name" value="O-FUCOSYLTRANSFERASE 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31288">
        <property type="entry name" value="O-FUCOSYLTRANSFERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10250">
        <property type="entry name" value="O-FucT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009360">
        <property type="entry name" value="UCP009360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0294">Fucose metabolism</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="67"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9673688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="585" mass="66910" checksum="3A592033902DC2EB" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D8X0</accession>
    <name>A0AA39D8X0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">laccase</fullName>
            <ecNumber evidence="5">1.10.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PVL29_024324</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>4 hydroquinone + O2 = 4 benzosemiquinone + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:11276"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17594"/>
            <dbReference type="ChEBI" id="CHEBI:17977"/>
            <dbReference type="EC" id="1.10.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Secreted</location>
            <location evidence="3">Extracellular space</location>
            <location evidence="3">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the multicopper oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.10.3.2" evidence="5"/>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D8X0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052716">
        <property type="term" value="F:hydroquinone:oxygen oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046274">
        <property type="term" value="P:lignin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13849">
        <property type="entry name" value="CuRO_1_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13875">
        <property type="entry name" value="CuRO_2_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13897">
        <property type="entry name" value="CuRO_3_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033138">
        <property type="entry name" value="Cu_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002355">
        <property type="entry name" value="Cu_oxidase_Cu_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034288">
        <property type="entry name" value="CuRO_1_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034285">
        <property type="entry name" value="CuRO_2_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034289">
        <property type="entry name" value="CuRO_3_LCC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF349">
        <property type="entry name" value="LACCASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00079">
        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00080">
        <property type="entry name" value="MULTICOPPER_OXIDASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0052">Apoplast</keyword>
    <keyword evidence="11" id="KW-0186">Copper</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0439">Lignin degradation</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0964">Secreted</keyword>
    <keyword evidence="14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041438942" description="laccase" evidence="14">
        <location>
            <begin position="19"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="17">
        <location>
            <begin position="39"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="15">
        <location>
            <begin position="166"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="16">
        <location>
            <begin position="457"/>
            <end position="589"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ9675326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="624" mass="70261" checksum="8C548748669807AE" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D8Y7</accession>
    <name>A0AA39D8Y7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Aspartic proteinase Asp1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Nucellin-like protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_023258</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D8Y7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05475">
        <property type="entry name" value="nucellin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000027">
        <property type="entry name" value="Aspartic proteinase Asp1 isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000015">
        <property type="entry name" value="Aspartyl protease family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033823">
        <property type="entry name" value="Nucellin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032799">
        <property type="entry name" value="TAXi_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032861">
        <property type="entry name" value="TAXi_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13683">
        <property type="entry name" value="ASPARTYL PROTEASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13683:SF227">
        <property type="entry name" value="EUKARYOTIC ASPARTYL PROTEASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14541">
        <property type="entry name" value="TAXi_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14543">
        <property type="entry name" value="TAXi_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Peptidase A1" evidence="11">
        <location>
            <begin position="68"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="86"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="286"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="325"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00068871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00077656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9673582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="453" mass="49111" checksum="2CC87C10AF0A4429" modified="2024-01-24" version="1">MIQSDSMGPSRLIKGRSAGSLLIEAMRFLLLSEMFLGCFSASKQPISNGLGHSVVFPLQGNVYPQGFYSVSLRIGNPPKPYTLDIDSGSDLTWLQCDAPCVSCTKAPHPPYKPNKGPITCNDPMCSALHWPSKPPCKASHEQCDYEVSYADHGSSFGVLVHDIFSLQLTNGTLAAPRLAFGCGYDQSYPGPNAPPFVDGVLGLGYGKSSIVTQLRSLGLIRSVVGHCLSGQGGGFLFLGDGLSTTPGIIWTPMSRKSGENAYVLGPADLLFNGQNSGVKGLRLVFDSGSSYTYFNAQAYKTTLSLVRKYLNGRLKETADESLPVCWRGAKPFKSIFEVKNYFKPFALSFTKAKNAQLQLPPESYLIISKHGNACFGILNGSEIGLGDSNVIGDIAFQDKMVIYDNERQQIGWVPKDCNKLPKVDRDYNKGFSPAVLGMVVEQFSAAYASREDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D986</accession>
    <name>A0AA39D986_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_024555</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D986"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34724:SF4">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34724">
        <property type="entry name" value="OS12G0596101 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9675686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="67" mass="7197" checksum="70B4C9DCE7DF09F7" modified="2024-01-24" version="1">MCYEVKCSTCGKTTWGGCGRHVPSVYERVQKGQHCLCRDWPGVKLGDQTTSSSTAADSQTNSSPATL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DBZ3</accession>
    <name>A0AA39DBZ3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Tubulin beta chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_021360</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="10">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the tubulin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DBZ3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000031">
        <property type="entry name" value="tubulin beta-4A chain isoform X5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013838">
        <property type="entry name" value="Beta-tubulin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002453">
        <property type="entry name" value="Beta_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01163">
        <property type="entry name" value="BETATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00228">
        <property type="entry name" value="TUBULIN_B_AUTOREG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7 10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 10" id="KW-0493">Microtubule</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="11">
        <location>
            <begin position="47"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9679403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="170" mass="18679" checksum="17D68E1134F4E41B" modified="2024-01-24" version="1">MREILHVQGGQCGNQIGAKFWEVVCAEHGIDSTGRYQGDTELQLERVNVYYNEASCGRFVPRAVLMDLEPGTMDSVRSGPYGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDAVRKEAENCDCLQGYQVCHSLGGGTGSGMGTLLISKIREENQNSLTPWLTWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DCD5</accession>
    <name>A0AA39DCD5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">F-box/kelch-repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_020547</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DCD5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000007">
        <property type="entry name" value="F-box/kelch-repeat protein SKIP11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052439">
        <property type="entry name" value="F-box/Kelch-repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46122">
        <property type="entry name" value="GALACTOSE OXIDASE/KELCH REPEAT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46122:SF6">
        <property type="entry name" value="OS04G0619300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9678400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="461" mass="51647" checksum="8C542F957EB6461E" modified="2024-01-24" version="1">MVFRYSLEELKMKIEGTVYESPAMVGVRGCSIFQLPSSFDGDLGFMLKNCYLLAYILLARQNNNKIKDVGKGLLNKEMYIPSLDCIGLKKKKPMIRAWAGENHGGQASDDSFLPGLNDDTALDIFAWSSRSDYSKLACLNKKFKSLIGTGYLYKLRRWLGVIEHWVYLACILMPWEAFDPERQRWMRLPRMPCDECFTYADKESLAVGTELLVFGRELSGFAIWMYSLLTRDWSRCPLMNLPRCLFGSSSLGEIAIVAGGSDKNGHVLKSAELYNSELGTWQTLPDMNLPRKLCSGFFMDGKFYVIGGMSSHTDCLSCGEEYNIETRTWRRIENMYPGSNIGTQFPPAMRSPPLVAVVNNQLYSADQATNEVKKYDKSNNSWSVVKRLPVRADSSNGWGLAFKACGDSLLVIGGHRGPEGEVIVLHSWEPEDGNAGGPEWNVLSVRERAGAFVYNCAVMGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DDW2</accession>
    <name>A0AA39DDW2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PLAC8 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_020301</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9681353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DDW2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31152">
        <property type="entry name" value="PLAC8 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31152:SF1">
        <property type="entry name" value="PLAC8 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="118"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9681353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="319" mass="36501" checksum="DC52E417D0454209" modified="2024-01-24" version="1">MATKQQLEKMSARQYYRNLWHTDLMHAISADCPYCCFATFCAPCASYMLRRRALYNDMSRYECCGGYMPCSGRCGESRCPEFCLCTEVFLCFANSVASTRFMLQDEFNIQTTKCDNCIIGFMFCLQQVACIFSLVAMIVGSDEIKSPPDLSCRSDICLLPDCPFSYWIVCHMLFSNDFEFILILHLCACKVCACMQISQSSTSWPPPSEPSYGRTVGYHPLLVKFKLIPCRWLSSSTAYPTPQSYRSTGFPPQAYPPQVLSSSGLPCHRLTHHPHNLMLTGELYHDHEDVRVEPLQNIDLIIQRSPWSFCLTNTEMDWY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DDW6</accession>
    <name>A0AA39DDW6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_019721</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DDW6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.330:FF:000001">
        <property type="entry name" value="Protein SRG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050295">
        <property type="entry name" value="Plant_2OG-oxidoreductases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47991">
        <property type="entry name" value="OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="6">
        <location>
            <begin position="293"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9680471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="451" mass="50824" checksum="02CD980F84A199E3" modified="2024-01-24" version="1">MAPIGIGLSVPSAIHIDAPILPSSFDTVSIPPDTSSVPLSDYLTDDTLSFSQSVKDTLPIVFTLEIPLCTRLVPKRDRQYLYLLPTAMESGLSSLGSSLLVPCVQELVKEPVTAVPHRYLRPEQDPPVLSNTTDSMPHVPIIDLYCLLSADFTHSELEKLHHACQEWGFFQLINHGVSSSLVEKVKAETQEFFNLPIDEKKKFWQKPGEVEGFGQAFVVSEEQKLDWGDLFFMSTLPTHFRKPHLFPKLPLPFRDTLEIYVVEMRNLALTMLSFMEKALKIEVNEMRELFEQGLQGMRMNYYPPCPQPEQVIGQTPHSDSVGLTILLQVNEVEGLQIRKDGMWIPITPLPNAFIVNIGDILEIFSNGIYKSIEHRAVVNSVKERLSIATFHSPQMDAEIGPVPSLITPEFPALFRRVGVADYFKKFFSRALDGKSYLEVLRIDGKEEGQNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DIR5</accession>
    <name>A0AA39DIR5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RNA 3'-terminal phosphate cyclase-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_019248</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RNA 3'-terminal cyclase family. Type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DIR5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000479">
        <property type="term" value="P:endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00875">
        <property type="entry name" value="RNA_Cyclase_Class_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.20">
        <property type="entry name" value="RNA 3'-terminal phosphate cyclase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.20">
        <property type="entry name" value="RNA 3'-terminal phosphate cyclase, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013791">
        <property type="entry name" value="RNA3'-term_phos_cycl_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023797">
        <property type="entry name" value="RNA3'_phos_cyclase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037136">
        <property type="entry name" value="RNA3'_phos_cyclase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000228">
        <property type="entry name" value="RNA3'_term_phos_cyc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016443">
        <property type="entry name" value="RNA3'_term_phos_cyc_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036553">
        <property type="entry name" value="RPTC_insert"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03400">
        <property type="entry name" value="18S_RNA_Rcl1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11096">
        <property type="entry name" value="RNA 3' TERMINAL PHOSPHATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11096:SF1">
        <property type="entry name" value="RNA 3'-TERMINAL PHOSPHATE CYCLASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01137">
        <property type="entry name" value="RTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05189">
        <property type="entry name" value="RTC_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="EPT/RTPC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="RNA 3'-terminal phosphate cyclase" evidence="5">
        <location>
            <begin position="8"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="RNA 3'-terminal phosphate cyclase insert" evidence="6">
        <location>
            <begin position="185"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9683602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="376" mass="41179" checksum="66B84EDE75C5AC0F" modified="2024-01-24" version="1">MAKTTYMKLKGSQNLRLRLLLATLSATPVLIDDIRHDDTWPGLRPYEVSLLRLFEKICDDCVVEINETGTKLKYKPGIIMGGKNLVHDCGVGRSIGYFLEPLIVLGLFGKKPLSITLKGITNDSKDPSVDTFQSTTLSILKRFGVPSEGLKLTIRNRAPPPHGGGEVELKIPIVQNSLTAVTWIDEGMVKRIRGLTFTSRVSSQFEHTMRYAARGIFNRLLPDVHISTYHSKTGKSPGYGISLVAETTSGCFISADTAVSYGRGEESGEDKDEENNELMPPEEVGEQIASVLLGEIEQGGVVDSTHQGLLFLLCALCPQDVSKIRVGKLSPNGIETLRHIRDFLGVKFVIKPDPSTETVILKCVGCGLKNLSRKIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DKY1</accession>
    <name>A0AA39DKY1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 15">3-isopropylmalate dehydrogenase</fullName>
            <ecNumber evidence="6 15">1.1.1.85</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_016004</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>(2R,3S)-3-isopropylmalate + NAD(+) = 4-methyl-2-oxopentanoate + CO2 + NADH</text>
            <dbReference type="Rhea" id="RHEA:32271"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17865"/>
            <dbReference type="ChEBI" id="CHEBI:35121"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.85"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="15">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="15">Binds 1 Mg(2+) or Mn(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 15">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 15">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.85" evidence="6 15"/>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DKY1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003862">
        <property type="term" value="F:3-isopropylmalate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:L-leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.718.10:FF:000004">
        <property type="entry name" value="3-isopropylmalate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.718.10">
        <property type="entry name" value="Isopropylmalate Dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01033">
        <property type="entry name" value="LeuB_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004429">
        <property type="entry name" value="Isopropylmalate_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00169">
        <property type="entry name" value="leuB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42979">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42979:SF1">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="14 15" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="7 15" id="KW-0432">Leucine biosynthesis</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-0464">Manganese</keyword>
    <keyword evidence="9 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 15" id="KW-0520">NAD</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="16">
        <location>
            <begin position="44"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9687340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="405" mass="43336" checksum="BC2E0CC5AA16E8BA" modified="2024-01-24" version="1">MAASLQLSARPFKPHFQSKSLSKPSSIRLATIRCSAATPPKRYSITLLPGDGIGPEVISVAKNVLKLAGSLEGIEFGFQEMPVGGCALDLTGVPLPEETLLVAKQSDAVLLGAIGGYKWDNNEKHLKPETGLLQLRSGLKVFANLRPASVLPQLVDASTLKKEVAEGVDLMVVRELTGGIYFGKPRGFGKNENGEEIGFNTEVYATYEIDRIARIAFETAQKRRGKLCSVDKANVLEASMLWRKRVSAMASEYPDVELSHMYVDNAAMQLVRNPKQFDTIVTNNIFGDILSDEASMITGSIGMLPSASVGESGPGLFEPIHGSAPDIAGQDKANPLATVLSAAMLLRYGLGEGNAAKRIETAVLDALDRGFRTGDIYEAGTKLVGCKEMGEEVLKAVESQIPAAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DLR5</accession>
    <name>A0AA39DLR5_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_014906</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9689453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DLR5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34130:SF3">
        <property type="entry name" value="DUF1645 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34130">
        <property type="entry name" value="OS08G0243800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9689453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="251" mass="27930" checksum="FEC3ACF1E93EAA56" modified="2024-01-24" version="1">MEVNDVVANVQNSEEEAEEALSLCDLPISPSETSGKDFSPCGRRSTSEPPELFEFFSDLSYEMSPADEIIFCGKLVPFKDAQTREIQDNKQGSVQKVSALRRRSESLSELQTSRSNSAKSRLVRNSRSLDYRKLYRSSSSQTSPGPEMDRNSSDKSSGRSDISASIRKVPKSRWYLLMFGLVKFPPEMELRDIKSRQVRRSPSTLFPSLDASVKGPVKRSSGKCSWGLLRALSCKDASVAVTASFHCIPRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DSQ6</accession>
    <name>A0AA39DSQ6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">S-acyltransferase</fullName>
            <ecNumber evidence="8">2.3.1.225</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Palmitoyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_010283</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="8">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="8"/>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DSQ6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12246">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="42"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="140"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="184"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="9">
        <location>
            <begin position="94"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9694734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="284" mass="31881" checksum="773FBB74760F24A6" modified="2024-01-24" version="1">MSPNYKFSLPVFVVASAITYIYFSTVFIFIDMWFGLTTSPGILNAIAFTAVAFMCVLNYVVAILTDPGRVPATFMPDIEDSQSPIHEIKRKGGDLRYCQKCAHYKPARAHHCRVCRRCVLRMDHHCIWINNCVGHANYKVFFIFVLYAVLGCIYSLILLVGSIYNDAEKDEEQTRGSFRNTYVISGLLLVPLSVALMVLLGWHIYLILQNKTTIEYHEGVRALYLAEKGGNVSKNFYDLGAYENLTSVLGPSIFSWVCPTSKHIGSGLRFPTTFDRAAGASMVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DTB5</accession>
    <name>A0AA39DTB5_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_008458</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DTB5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="24"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9696218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="86" mass="9466" checksum="518531D5EC262386" modified="2024-01-24" version="1">MPMALLARPSRRKVGAQEKRNFDTETTSSASSPPVAVRVTVLSGKTPHVLLLHPGRGAEKQSPQPLALANEHWADRHRAGRYEEDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DTM5</accession>
    <name>A0AA39DTM5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tubulin/FtsZ GTPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_010927</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the tubulin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DTM5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002453">
        <property type="entry name" value="Beta_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01163">
        <property type="entry name" value="BETATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0493">Microtubule</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="5">
        <location>
            <begin position="29"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9695694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="126" mass="13808" checksum="7576B5FDD59954CD" modified="2024-01-24" version="1">MAKICLLKGRAPTFFNSRMWGTHKGKETRFRNVGLEPDATDSIRTGPYGQVFRPDNFVFGQSGAGNNWAKGHYIEGAKLIDSVLDVVRKEIENCDYLQGSLPLAIGKTQPGLCSAIAPTASSWSHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DTU2</accession>
    <name>A0AA39DTU2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase Ty1/copia-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_009201</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DTU2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47481">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47481:SF22">
        <property type="entry name" value="RETROTRANSPOSON GAG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase Ty1/copia-type" evidence="2">
        <location>
            <begin position="500"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="406"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="406"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9697301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="968" mass="107249" checksum="CB77E79F06CAFA6D" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DUI6</accession>
    <name>A0AA39DUI6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_007272</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclic nucleotide-gated cation channel (TC 1.A.1.5) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DUI6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005216">
        <property type="term" value="F:monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.630:FF:000003">
        <property type="entry name" value="Cyclic nucleotide-gated ion channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000024">
        <property type="entry name" value="Cyclic nucleotide-gated ion channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.630">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45651:SF12">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-GATED ION CHANNEL 15-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45651">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-GATED ION CHANNEL 15-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0407">Ion channel</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="95"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="184"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="226"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="255"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="12">
        <location>
            <begin position="447"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="649"/>
            <end position="677"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9698081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="677" mass="77271" checksum="A814F1DC03338899" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DUQ7</accession>
    <name>A0AA39DUQ7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Dienelactone hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_008480</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DUQ7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000178">
        <property type="entry name" value="Carboxymethylenebutenolidase homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002925">
        <property type="entry name" value="Dienelactn_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17630">
        <property type="entry name" value="DIENELACTONE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17630:SF44">
        <property type="entry name" value="PROTEIN AIM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01738">
        <property type="entry name" value="DLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dienelactone hydrolase" evidence="3">
        <location>
            <begin position="29"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9696265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="236" mass="25298" checksum="73DAA901E5390B3F" modified="2024-01-24" version="1">MSGPHCFENPPTISSSSGAGCVAEIGGLKAYVSGPSDSKLAILLVSDAFGYEAPNLRNLADKVAGAGFYVVVPDFFYGDPFSPEANVPVWLKAHGTDKGFEDAKPIIAELRSKGINAIGAAGFCWGAKVVVELSKAGHIQAAVLLHPSLVTVDDIKEIKAPIAILGAENDHSSPPELLKQFEEVLFTKPEVNGYVKIFPGVAHGWSVRYKVEDEEAVKQADEAHQNTMDWFAQYVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DW33</accession>
    <name>A0AA39DW33_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">V-type proton ATPase subunit S1/VOA1 transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_006988</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9697645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DW33"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046756">
        <property type="entry name" value="VAS1/VOA1_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35285">
        <property type="entry name" value="2-C-METHYL-D-ERYTHRITOL 4-PHOSPHATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35285:SF1">
        <property type="entry name" value="2-C-METHYL-D-ERYTHRITOL 4-PHOSPHATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20520">
        <property type="entry name" value="Ac45-VOA1_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="430"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="V-type proton ATPase subunit S1/VOA1 transmembrane" evidence="6">
        <location>
            <begin position="426"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF20520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9697645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="462" mass="51009" checksum="34446BB766945ACA" modified="2024-01-24" version="1">MGLELNTNKNMGREGINEQERCTRVNVEELADGLNSIHMTIHRRGSIFTGFFTLDLCKVQSSHADWWCSGPHNSLSNSVAEDEVVATIVEKVKVSVEDRLIVETLIPSEGGARPQILDEWEHLETVDFTIPLQVAYTRPPIMPSVKMKTFVVSLLMVLLVVSRVPYRTALPSTVPAFLWSPHQIEMKEAVNYQTLSPKDLAKSVVSEGGWSNLLCSGEKDQQPVDLALVFVGRELSSLDISGSKHADPALVDLLKVSFARSNFSMAFPYVAVSEEKEAMENSLILGFTETCGHDLGVSNVAFLESCSVEGGNFKKLADLHSVHDYLVSRMETRPVGPTDLVMFCHGGSHLEELDQQRLESEVFSELISSLEQSGTKYTVLYVSDPFRSIQYPSYRDLERFLAEGTYGNGSANSTTCDEVCQIKSSLLEGLLVGIVLLIILISGLCCMMGIDTPTRFEAPQDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DW84</accession>
    <name>A0AA39DW84_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_005758</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the mTERF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DW84"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.70.10:FF:000001">
        <property type="entry name" value="Mitochondrial transcription termination factor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.70.10">
        <property type="entry name" value="Transcription termination factor 3, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003690">
        <property type="entry name" value="MTERF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038538">
        <property type="entry name" value="MTERF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13068">
        <property type="entry name" value="CGI-12 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13068:SF166">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR MTERF15, MITOCHONDRIAL-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02536">
        <property type="entry name" value="mTERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00733">
        <property type="entry name" value="Mterf"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0806">Transcription termination</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041350681" evidence="4">
        <location>
            <begin position="22"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9700076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="394" mass="45126" checksum="A500415457C81992" modified="2024-01-24" version="1">MRMMVGFLRKALVLQVRFTLAASPTPQSLHFLPHHFYPFSSSSTSISTSERSESFTVSYLIDSCGLSHKDALSASKLFRFETPEKPDSVLAFFNSHGFSKSQTSKIVKSLPRLLSSDPDKTLLPKLQFFYSKGASKPDVAKIVVSTPGILKRSLENQIIPSFNFLKDFLQSDEMAITVVKRFSRIMLFDLHTYVASNMSALQEFGVPKSNIAGLLMYRPMAFMVNPNLFRKNLEEVKKMGFNPSQMKFVLAIQAMRAGGESCWERKIDIYKRWGWSEEEIRLAFTKSPWCMIYSEDKIMAKMDFFVNIMGRESSLIAHRPFLIGLSLEKRIIPRYSVVQVLLSKGLINKDISLVVLFESTEKTFLERFVNAYKEEAPQLMKLYQEKINLSEKQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DWU7</accession>
    <name>A0AA39DWU7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein root UVB sensitive 2, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_008918</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RUS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DWU7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009941">
        <property type="term" value="C:chloroplast envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009926">
        <property type="term" value="P:auxin polar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010224">
        <property type="term" value="P:response to UV-B"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006968">
        <property type="entry name" value="RUS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055412">
        <property type="entry name" value="UVB_sens_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054549">
        <property type="entry name" value="UVB_sens_RUS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12770:SF5">
        <property type="entry name" value="PROTEIN ROOT UVB SENSITIVE 2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12770">
        <property type="entry name" value="RUS1 FAMILY PROTEIN C16ORF58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24160">
        <property type="entry name" value="UVB_sens_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04884">
        <property type="entry name" value="UVB_sens_prot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein root UVB sensitive/RUS" evidence="2">
        <location>
            <begin position="51"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Root UVB sensitive protein C-terminal" evidence="3">
        <location>
            <begin position="294"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9696922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="423" mass="46554" checksum="0C6ACA7C2068E140" modified="2024-01-24" version="1">MKKKMKEQERAVAQGAVFWIETSDSVSRHYQFHSNGDLSMKVVDDSRTVVHRVVESFLNKFFPSGYPYSVNEGYLRYTQFRALQHFSSAALSVLSTQSLLFAAGLRPTPAQATAVSWVLKDGMQHVGKLICSNLGARMDSEPKLWRILADVLYDLGTGLEVLSPLCPHLFLEMAGLGNFAKGMAVVAARATRLPIYSSFAKEGNLSDLFAKGEAISTLFNVVGMGAGIQLASTICSSIQGKMIAGPLLSVIHVYSVIEEMRAAPVNTLNPQRTAMIVADFIKTGKISSPADLRYQEDLIFTGRLIEDAGNVKVGRDLHKVVKPSKLCKLKGVFPEEKFLLCHGKKWTDMVLEHDATGEDALRGWLVAAYAANLEKTGHEPSMTVLQEAYDKMNSVFSTFVSKLQAKGWHTDCFLDGTGSRFAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DXC5</accession>
    <name>A0AA39DXC5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Disease resistance RPP13-like protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_009311</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14798">
        <property type="entry name" value="RX-CC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057135">
        <property type="entry name" value="At4g27190-like_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038005">
        <property type="entry name" value="RX-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766:SF51">
        <property type="entry name" value="DISEASE RESISTANCE RPP13-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766">
        <property type="entry name" value="PLANT BROAD-SPECTRUM MILDEW RESISTANCE PROTEIN RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23247">
        <property type="entry name" value="LRR_RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="6">
        <location>
            <begin position="14"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="5">
        <location>
            <begin position="190"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="8">
        <location>
            <begin position="444"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="9">
        <location>
            <begin position="555"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein At4g27190-like leucine-rich repeats" evidence="7">
        <location>
            <begin position="823"/>
            <end position="951"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9697432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1286" mass="145695" checksum="7B4A38E7D915E4B7" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DXT6</accession>
    <name>A0AA39DXT6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_006253</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DXT6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025724">
        <property type="entry name" value="GAG-pre-integrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039537">
        <property type="entry name" value="Retrotran_Ty1/copia-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057670">
        <property type="entry name" value="SH3_retrovirus"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648">
        <property type="entry name" value="TRANSPOSASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648:SF28">
        <property type="entry name" value="TRANSPOSON-ENCODED PROTEIN WITH RIBONUCLEASE H-LIKE AND RETROVIRUS ZINC FINGER-LIKE DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13976">
        <property type="entry name" value="gag_pre-integrs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25597">
        <property type="entry name" value="SH3_retrovirus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="4">
        <location>
            <begin position="69"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="652"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="657"/>
            <end position="671"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9700838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="883" mass="99830" checksum="639C352F457064F9" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DXZ0</accession>
    <name>A0AA39DXZ0_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_006358</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DXZ0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090730">
        <property type="term" value="C:Las1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000460">
        <property type="term" value="P:maturation of 5.8S rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000470">
        <property type="term" value="P:maturation of LSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007174">
        <property type="entry name" value="Las1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15002">
        <property type="entry name" value="RIBOSOMAL BIOGENESIS PROTEIN LAS1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15002:SF0">
        <property type="entry name" value="RIBOSOMAL BIOGENESIS PROTEIN LAS1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="438"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9700988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="542" mass="60941" checksum="02129085428DCAE7" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DY40</accession>
    <name>A0AA39DY40_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">DNA-binding protein S1FA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_007322</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-binding protein that specifically recognizes a negative element (S1F) within the RPS1 promoter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the S1FA transcription factor family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DY40"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006779">
        <property type="entry name" value="S1FA_DNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35298:SF12">
        <property type="entry name" value="DNA-BINDING PROTEIN S1FA-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35298">
        <property type="entry name" value="DNA-BINDING PROTEIN S1FA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04689">
        <property type="entry name" value="S1FA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="34"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="64"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="64"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9698182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="89" mass="9964" checksum="2F4CF95E158E0B1B" modified="2024-01-24" version="1">MEEDFEFADKVPPSFERMGNVIKDADAKGFNPGLIVLLLVVGLLLVFLVGNYALYVYAQKTLPPRKKKPVSKKKMKRERLKQGVSAPGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E0L5</accession>
    <name>A0AA39E0L5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_003301</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E0L5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001306">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At1g28690, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000184">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:002162">
        <property type="entry name" value="Putative pentatricopeptide repeat-containing protein, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF436">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN ELI1, CHLOROPLASTIC-LIKE ISOFORM X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="199"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="383"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="414"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9705176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="630" mass="70675" checksum="013ADBFA1881A8A9" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E0V0</accession>
    <name>A0AA39E0V0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_004782</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E0V0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10527">
        <property type="entry name" value="SET_LSMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1410.10">
        <property type="entry name" value="set domain protein methyltransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050600">
        <property type="entry name" value="SETD3_SETD6_MTase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271:SF55">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271">
        <property type="entry name" value="UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9703149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="572" mass="64710" checksum="666B25C0A33BCD34" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E1M2</accession>
    <name>A0AA39E1M2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Strictosidine synthase conserved region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_005247</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the strictosidine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E1M2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018119">
        <property type="entry name" value="Strictosidine_synth_cons-reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10426:SF88">
        <property type="entry name" value="ADIPOCYTE PLASMA MEMBRANE-ASSOCIATED PROTEIN HEMOMUCIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10426">
        <property type="entry name" value="STRICTOSIDINE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20067">
        <property type="entry name" value="SSL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03088">
        <property type="entry name" value="Str_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63829">
        <property type="entry name" value="Calcium-dependent phosphotriesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0926">Vacuole</keyword>
    <feature type="domain" description="Strictosidine synthase conserved region" evidence="6">
        <location>
            <begin position="149"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9703884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="315" mass="34799" checksum="8220C34982A9CF34" modified="2024-01-24" version="1">MADPTPSQSSMDRRSSSWPLGLLTLILAPVASAMLLYQLDPMTQLLFPSTRSEMIGVGKLLGPEDIAYHPDSHLIYTGCADGWVKRVTLNDSMVQNWAFTGGRPLGVALGRHGQLIVADAEKGLLEVTTDGMVKTLTDEAEGLKFKLTDSVDVAVDGMIYFTDASYKYSLKEYIWDILEGRPHGRLMSFDPSTKETKVLVLDLFFANGVIVPPDQNSVILCEIGNSLAWDLVLKYPWIRKVVAIMERYKVRPHMEKNGGVLVVDLEGNPAAYYYDSGLSEVTSGMKIGNHLYCGSITAPYMIRLDLHQHAAHATM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YGU6</accession>
    <name>A0AA38YGU6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Homeobox-leucine zipper protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">HD-ZIP protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Homeodomain transcription factor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_026615</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Transcription factor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 9">Belongs to the HD-ZIP homeobox family. Class I subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YGU6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000159">
        <property type="entry name" value="Homeobox-leucine zipper protein HAT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045224">
        <property type="entry name" value="HDZip_class_I_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000047">
        <property type="entry name" value="HTH_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003106">
        <property type="entry name" value="Leu_zip_homeo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24326">
        <property type="entry name" value="HOMEOBOX-LEUCINE ZIPPER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24326:SF547">
        <property type="entry name" value="HOMEOBOX-LEUCINE ZIPPER PROTEIN ATHB-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02183">
        <property type="entry name" value="HALZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00031">
        <property type="entry name" value="HTHREPRESSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 8" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="55"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="184"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="125"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="195"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU369038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9670175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="337" mass="37894" checksum="610F02AAE97E32E5" modified="2024-01-24" version="1">MKRLGSSESLGALMAICPTTDEHSPRNNHVYSREFQTMFDGLDEEGCVEESGQIVEKKRRLSVDQVKALEKNFEVENKLEPERKVKLAQELGLQPRQVAVWFQNRRARWKTKQLERDYGILKANYETLKLNYDALQHDNEALLKEIRELKSKLNEEKTESNLSVKEELVVSESDEKVKVMEQSETAMGAGIGGSDAKDLSNDDSFKDGNGGGRVSVFPDMKDGSSDSDSSAILNEDNSPIAAISSSGVLQNNHHHHLLMSPPSSSLRFNCSSSSSSINCFHFSDSSRPILGNNTQKAYQPQFVKMEEHNFFGADESCNFFSDDQPPTLPWYCPDQWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YJB5</accession>
    <name>A0AA38YJB5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Glutamate receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_025293</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Glutamate-gated receptor that probably acts as non-selective cation channel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YJB5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015276">
        <property type="term" value="F:ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13686">
        <property type="entry name" value="GluR_Plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19990">
        <property type="entry name" value="PBP1_GABAb_receptor_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000172">
        <property type="entry name" value="Glutamate receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000514">
        <property type="entry name" value="Glutamate receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000188">
        <property type="entry name" value="Glutamate receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044440">
        <property type="entry name" value="GABAb_receptor_plant_PBP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015683">
        <property type="entry name" value="Ionotropic_Glu_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001320">
        <property type="entry name" value="Iontro_rcpt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017103">
        <property type="entry name" value="Iontropic_Glu_rcpt_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18966">
        <property type="entry name" value="IONOTROPIC GLUTAMATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00060">
        <property type="entry name" value="Lig_chan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037090">
        <property type="entry name" value="Iontro_Glu-like_rcpt_pln"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00079">
        <property type="entry name" value="PBPe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 13" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 13" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="8 13 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 13" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="534"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="595"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="773"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor C-terminal" evidence="16">
        <location>
            <begin position="416"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="700"/>
            <end position="755"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR037090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR037090-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9671518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="845" mass="94379" checksum="93F029D4B62ACCA5" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YJG6</accession>
    <name>A0AA38YJG6_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_025354</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YJG6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9671599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="56" mass="5957" checksum="89323ECDA1A6E442" modified="2024-01-24" version="1">MKFLFQCPCCSCFCFMKPKKGKPAVKQAASAATEATKEAKVEAKEAAKGAKEAKAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YK03</accession>
    <name>A0AA38YK03_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Receptor-like serine/threonine-protein kinase</fullName>
            <ecNumber evidence="17">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">PVL29_025506</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="24">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="17"/>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YK03"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048544">
        <property type="term" value="P:recognition of pollen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00028">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01098">
        <property type="entry name" value="PAN_AP_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000060">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.90.10.10:FF:000004">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000195">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.4.10:FF:000002">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.10">
        <property type="entry name" value="Bulb-type lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.4.10">
        <property type="entry name" value="Hepatocyte Growth Factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021820">
        <property type="entry name" value="S-locus_recpt_kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000858">
        <property type="entry name" value="S_locus_glycoprot_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024171">
        <property type="entry name" value="SRK-like_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32444:SF183">
        <property type="entry name" value="APPLE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32444">
        <property type="entry name" value="BULB-TYPE LECTIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11883">
        <property type="entry name" value="DUF3403"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08276">
        <property type="entry name" value="PAN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00954">
        <property type="entry name" value="S_locus_glycop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000641">
        <property type="entry name" value="SRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00473">
        <property type="entry name" value="PAN_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 17 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 17 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 17" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 20" id="KW-0732">Signal</keyword>
    <keyword evidence="4 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041267172" description="Receptor-like serine/threonine-protein kinase" evidence="20">
        <location>
            <begin position="20"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="435"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="22">
        <location>
            <begin position="20"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="23">
        <location>
            <begin position="337"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="498"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="526"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRNR" id="PIRNR000641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KAJ9671834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="812" mass="90818" checksum="6CB485015DA3ED4E" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YKY3</accession>
    <name>A0AA38YKY3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_025825</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YKY3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11072">
        <property type="entry name" value="CYP71-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000008">
        <property type="entry name" value="Cytochrome P450 71D8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052306">
        <property type="entry name" value="CYP450_71D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47953:SF19">
        <property type="entry name" value="OS06G0641600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47953">
        <property type="entry name" value="OS08G0105600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12 13" id="KW-0349">Heme</keyword>
    <keyword evidence="9 12 13" id="KW-0408">Iron</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 13" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="8 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="binding site" description="axial binding residue" evidence="12">
        <location>
            <position position="446"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9672357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="509" mass="58009" checksum="EC5188DD1116FB71" modified="2024-01-24" version="1">MELYFPSFHILSTFILFLVVVLRTHKRSKTSSLTPNLPPGPWKLPLIGNIHQLVGSLPHHALGDLAKKYGPLMHLQLGEVSTIVVSSSEIAKEVMKSHDIIFAQRPHILATRIMSYNSTNIAFAPYGDYWRHLRKICMSELLSANRVQSFQSIRNEEESNLVRSISLNTGSPINLTEKTFASICAITTRAAFGKKCKYQETFISVLLETIKLAGGFNVGDIFPSFKFLHLISGMRPKLEKLHQEADKILENIIHEHKARGGTTKIDKDEPDEDLVDVLLKFHEEHGNHAFSLTTDNIKAVLLDIFGAGSEPSSTTIDFAMSEMMRNPRIMRKAQEEVRRVFDRKEEIDEMGIQELKFLKLVIKETLRLHPPLPLLLPRECREKCEIDGHEIPVKSKIIVNAWAIGRDPKHWTEPESFNPERFLDSSIDYKGTNFEYIPFGAGRRICPGILFGLASVELLLAKLLYHFDWKLPNGMKQQDLDMTEVFGLAVRRKEDLYLIPTAYYPLSHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YM04</accession>
    <name>A0AA38YM04_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_026247</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YM04"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032843">
        <property type="entry name" value="Jiv"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45270:SF1">
        <property type="entry name" value="CHAPERONE DNAJ-DOMAIN SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45270">
        <property type="entry name" value="OS03G0832900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14901">
        <property type="entry name" value="Jiv90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="161"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="289"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9672912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="551" mass="63033" checksum="DA839F0F95462DFA" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YNG2</accession>
    <name>A0AA38YNG2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GAG-pre-integrase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_023325</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YNG2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025724">
        <property type="entry name" value="GAG-pre-integrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054722">
        <property type="entry name" value="PolX-like_BBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47592:SF27">
        <property type="entry name" value="OS08G0421700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47592">
        <property type="entry name" value="PBF68 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13976">
        <property type="entry name" value="gag_pre-integrs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22936">
        <property type="entry name" value="Pol_BBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Retrovirus-related Pol polyprotein from transposon TNT 1-94-like beta-barrel" evidence="3">
        <location>
            <begin position="126"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="GAG-pre-integrase" evidence="2">
        <location>
            <begin position="232"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9673695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="353" mass="39153" checksum="8AE75EE24721F8DC" modified="2024-01-24" version="1">MLWEHITSGPEASALVTHGPHTGSSPRQSKTTYCEHCKKMAHTKDTCWTLHGKPVDWKPKQPNKAHENHQSTSSVGFNFDQLANLYELLSNFQASGQSSTTLSSGSLAHKGNFWTTLSTTSHITPWIIDSGASDHMIDAHHLFSTYSPCAGNLKVKIADGTLSPVAGKGSIRISKSITLNPILHVPNLSCNLLSISQLTKQSNCSTKFLPSHCVFQDLSSGKTIGSAKEREGLYNFDEANVSGQCLVCNSSSSPGESELLLWHKRMGHPSFQYWQHLFPSLCSNKASWDFQCEVCELAKHHHASFPKSKYKPFIPFTLIHSDLWGPSRTPNRTHKKWFITLLMITLTCVGYIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YNI9</accession>
    <name>A0AA38YNI9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">YbaK/aminoacyl-tRNA synthetase-associated domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_023303</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PRORSD1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YNI9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04335">
        <property type="entry name" value="PrdX_deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.960.10:FF:000005">
        <property type="entry name" value="Putative prolyl-tRNA synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.960.10">
        <property type="entry name" value="YbaK/aminoacyl-tRNA synthetase-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040285">
        <property type="entry name" value="ProX/PRXD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036754">
        <property type="entry name" value="YbaK/aa-tRNA-synt-asso_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007214">
        <property type="entry name" value="YbaK/aa-tRNA-synth-assoc-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31423:SF3">
        <property type="entry name" value="PROLYL-TRNA SYNTHETASE ASSOCIATED DOMAIN-CONTAINING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31423">
        <property type="entry name" value="YBAK DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04073">
        <property type="entry name" value="tRNA_edit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55826">
        <property type="entry name" value="YbaK/ProRS associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YbaK/aminoacyl-tRNA synthetase-associated" evidence="3">
        <location>
            <begin position="24"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="191"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9673663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="320" mass="35026" checksum="14693094D2B827FE" modified="2024-01-24" version="1">MGHSKDQLLARLQELQIDFTKYEHPAVLTVEAQEKYVGHLGGGLSKNLFLKDKKHRFYIVSALKDTKVDLKVLSQRLGLGKGGLRMAPEEALGEILQVSLGCVTPFALVNESARHVSLFLDQGFKTEDFCFFHPLSNDMSIAIKACDLDKFLKSIGKEPSYVDLEANPTVGKDQPPDLAGLVPSDAIVLQDPPEKSVSSGPTEKHASGDNKSSVAAAKTVKPSNNAQKVKEKPASSVHQLSSFTDPGHFVEEILDKTSAIVLSEITEDAIKQHGEHLGAVLSDSIRKRLSLDLKNLAIIYKNTAYTEGFHAATHRQRKHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YNL8</accession>
    <name>A0AA38YNL8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_023312</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YNL8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009813">
        <property type="term" value="P:flavonoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046148">
        <property type="term" value="P:pigment biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.330:FF:000009">
        <property type="entry name" value="Flavonol synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050231">
        <property type="entry name" value="Iron_ascorbate_oxido_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47990">
        <property type="entry name" value="2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="8" id="KW-0284">Flavonoid biosynthesis</keyword>
    <keyword evidence="7 9" id="KW-0408">Iron</keyword>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0847">Vitamin C</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="10">
        <location>
            <begin position="196"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9673679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="335" mass="38300" checksum="BE01F8C009E7E8AC" modified="2024-01-24" version="1">MDVERVQTIAFSSMTEGRIPPEFIRSEKERPAITTLHGYDPQIPTIDFSDPDEDSLTRLIAEASRDWGMFQIVNHGIPTHVINNLQKVGEDFFALPQEEKELYAKPPDSKSIEGYGTRLQKEEEGKKAWVDHLFHKIWPPSAINYQFWPKNPPSYRDANEAYAKCLRGVADKLFGRLSVGLGLGEKELRESVGGDELTYLLKINYYPPCPRPDLALGVVAHTDMSAITILVPNHVQGLQLFRDDHWFDVKYIPNALVIHIGDQLEILSNGKYKSVLHRTTVNKDMTRMSWPVFLEPPPELAIGPLPKLISEDNPPKYKKKRYCDYVYCKLNKIPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YRT5</accession>
    <name>A0AA38YRT5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Tryptophan aminotransferase-related protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_024426</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the alliinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YRT5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016846">
        <property type="term" value="F:carbon-sulfur lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.30">
        <property type="entry name" value="EGF-like, alliinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006948">
        <property type="entry name" value="Alliinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037029">
        <property type="entry name" value="Alliinase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006947">
        <property type="entry name" value="EGF_alliinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050478">
        <property type="entry name" value="Ethylene_sulfur-biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43795">
        <property type="entry name" value="BIFUNCTIONAL ASPARTATE AMINOTRANSFERASE AND GLUTAMATE/ASPARTATE-PREPHENATE AMINOTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43795:SF20">
        <property type="entry name" value="TRYPTOPHAN AMINOTRANSFERASE-RELATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04864">
        <property type="entry name" value="Alliinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04863">
        <property type="entry name" value="EGF_alliinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="domain" description="Alliinase EGF-like" evidence="6">
        <location>
            <begin position="38"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="Alliinase C-terminal" evidence="7">
        <location>
            <begin position="93"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9675498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="458" mass="50657" checksum="AD8CBEF899199238" modified="2024-01-24" version="1">MGKIQNCMYVVCLLCSVTLNILFVGRDVYVSGKWEVGWSRRAAEEAEAVAAVSCSGHGRAFLDGLVVDGSPVCECNTCFGGPDCSQFSASCVADVDSGDPLFLEPFWMQHAASSAVLVAGWHRMSYSFNDHSSISQELVKLIRKLHAIVGNANTTGRFIVFGAGSTQLLNAAVHALSAHNSSAPAKVVATIPFYPVYKSQTDFFRSVDFQFAGDTSLWKNSTSDPTSNLIEFVTAPNNPDGKLNKAVLQGPNAKPIYDHAYYWPHFTPIPAPADEDLMIFTISKLTGHAGSRFGWALIKDKDVYETMSNYMSLNTLGVSRDTQLRAFQLLKVVTQRRGREIFEFGYKTMKDRWEKLNKALLMSRRFSVQENSPQYCTFSGKVKRPSPAYAWLKCEKEEDKECYGVLKKAGIIGRNGTLFGANSSYVRLSLIKTQDDFDILLHQINKLVGEEDGGKSSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YSY7</accession>
    <name>A0AA38YSY7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase</fullName>
            <ecNumber evidence="3">3.2.2.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_024891</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>NAD(+) + H2O = ADP-D-ribose + nicotinamide + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16301"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57967"/>
            <dbReference type="EC" id="3.2.2.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:16302"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the disease resistance TIR-NB-LRR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.6" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9676111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061809">
        <property type="term" value="F:NAD+ nucleosidase activity, cyclic ADP-ribose generating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050832">
        <property type="term" value="P:defense response to fungus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043068">
        <property type="term" value="P:positive regulation of programmed cell death"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10140:FF:000007">
        <property type="entry name" value="Disease resistance protein (TIR-NBS-LRR class)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045344">
        <property type="entry name" value="C-JID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011713">
        <property type="entry name" value="Leu-rich_rpt_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058546">
        <property type="entry name" value="RPS4B/Roq1-like_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058192">
        <property type="entry name" value="WHD_ROQ1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017:SF570">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN (TIR-NBS CLASS)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20160">
        <property type="entry name" value="C-JID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23286">
        <property type="entry name" value="LRR_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07725">
        <property type="entry name" value="LRR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23282">
        <property type="entry name" value="WHD_ROQ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0611">Plant defense</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="TIR" evidence="14">
        <location>
            <begin position="23"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1307"/>
            <end position="1416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1322"/>
            <end position="1331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1337"/>
            <end position="1359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1374"/>
            <end position="1383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1389"/>
            <end position="1404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00061488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9676111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1416" mass="163186" checksum="3091B100FA60326A" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YTY5</accession>
    <name>A0AA38YTY5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DYW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_021826</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. PCMP-H subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9676483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YTY5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000242">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032867">
        <property type="entry name" value="DYW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF360">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14432">
        <property type="entry name" value="DYW_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="145"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="180"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="246"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="380"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="DYW" evidence="4">
        <location>
            <begin position="458"/>
            <end position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9676483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="550" mass="62345" checksum="7C7816B840EF27F9" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YUA4</accession>
    <name>A0AA38YUA4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Potassium channel</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PVL29_021977</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Potassium channel.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">The potassium channel is composed of a homo- or heterotetrameric complex of pore-forming subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Membrane</location>
            <topology evidence="1 16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="16">The KHA domain (rich in hydrophobic and acidic residues) present in the C-terminal part is likely to be important for tetramerization.</text>
    </comment>
    <comment type="domain">
        <text evidence="16">The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids. The pore-forming region H5 is enclosed by the transmembrane segments S5 and S6 in the Shaker-type (1P/6TM) and contains the GYGD signature motif which seems to be involved in potassium selectivity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the potassium channel family. Plant (TC 1.A.1.4) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9676730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YUA4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000074">
        <property type="entry name" value="Potassium channel KAT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000139">
        <property type="entry name" value="Potassium channel SKOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003938">
        <property type="entry name" value="K_chnl_volt-dep_EAG/ELK/ERG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045319">
        <property type="entry name" value="KAT/AKT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021789">
        <property type="entry name" value="KHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45743:SF11">
        <property type="entry name" value="POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45743">
        <property type="entry name" value="POTASSIUM CHANNEL AKT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13857">
        <property type="entry name" value="Ank_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11834">
        <property type="entry name" value="KHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01463">
        <property type="entry name" value="EAGCHANLFMLY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51490">
        <property type="entry name" value="KHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 15" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="14 16" id="KW-0407">Ion channel</keyword>
    <keyword evidence="12 16" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 16" id="KW-0630">Potassium</keyword>
    <keyword evidence="7 16" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4 16" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 16" id="KW-0813">Transport</keyword>
    <keyword evidence="8 16" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="87"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="123"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="226"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="280"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="311"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="18">
        <location>
            <begin position="411"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="15">
        <location>
            <begin position="588"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="15">
        <location>
            <begin position="621"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="15">
        <location>
            <begin position="685"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="domain" description="KHA" evidence="19">
        <location>
            <begin position="765"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU369015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAJ9676730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="838" mass="95406" checksum="0338178B7D753F65" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YUV2</accession>
    <name>A0AA38YUV2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PVL29_022107</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9676952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YUV2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000590">
        <property type="entry name" value="PR5-like receptor kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000178">
        <property type="entry name" value="serine/threonine-protein kinase PBS1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045874">
        <property type="entry name" value="LRK10/LRL21-25-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032872">
        <property type="entry name" value="WAK_assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025287">
        <property type="entry name" value="WAK_GUB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27009">
        <property type="entry name" value="RUST RESISTANCE KINASE LR10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13947">
        <property type="entry name" value="GUB_WAK_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14380">
        <property type="entry name" value="WAK_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 17" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041350217" description="non-specific serine/threonine protein kinase" evidence="17">
        <location>
            <begin position="33"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="281"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="351"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="379"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ9676952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="659" mass="73735" checksum="24B9DFE9592A0780" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YVP4</accession>
    <name>A0AA38YVP4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Flavin-containing monooxygenase</fullName>
            <ecNumber evidence="8">1.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_022472</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the conversion of methylthioalkyl glucosinolates of any chain length into methylsulfinylalkyl glucosinolates.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the FMO family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.-.-.-" evidence="8"/>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YVP4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004499">
        <property type="term" value="F:N,N-dimethylaniline monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000147">
        <property type="entry name" value="Flavin-containing monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000960">
        <property type="entry name" value="Flavin_mOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020946">
        <property type="entry name" value="Flavin_mOase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050346">
        <property type="entry name" value="FMO-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23023">
        <property type="entry name" value="DIMETHYLANILINE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00743">
        <property type="entry name" value="FMO-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000332">
        <property type="entry name" value="FMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00370">
        <property type="entry name" value="FMOXYGENASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0274">FAD</keyword>
    <keyword evidence="3 8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="6 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00058243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9677494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="458" mass="52150" checksum="4794CF994C1DE1B4" modified="2024-01-24" version="1">MQSERKQSKYVCVIGAGPSGLVTTRELRKEGHCVVMMEQNHDVGGQWLYDPNVEGEDPLGRSKFLKVHSSVYASLRLATPREVLGFSDFPFVVKKGRDTRRFPGHRELLWYLEDFCEWFGLRETIRFNTRVEYVGMLDSEVGGGLKWVVRSRDVKSEKVVEELFDAVVVATGQYSHPRLPSIKGMEAWKRKQMHSHIYRFPHPFHNEVVVVVGNSVSGRDISMELVDVAKEIHLGAKSLDISEGLSKVISKHHNLHLHLQIESLHEDGRVVFVDGSWVMADTIIYCTGYSYAFPFLDTKGIVGVGDDRVGPLYEHTFPPSLAPSLSSVGIPRKIIGFPFFESQAIWIAQLLSGRKTLPSFHDMMQSIEDFYRSRDAAGIPKHHTRDIADFEYCDKYLDNVGFPHLEEWRKELILSGIGNAQVNLETYRDAWDDHELLQVALQSPHFTQSLQSPSQHFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YW30</accession>
    <name>A0AA38YW30_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">X8 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_022520</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YW30"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009506">
        <property type="term" value="C:plasmodesma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1040:FF:000001">
        <property type="entry name" value="Glucan endo-1,3-beta-glucosidase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012946">
        <property type="entry name" value="X8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044788">
        <property type="entry name" value="X8_dom_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31044">
        <property type="entry name" value="BETA-1,3 GLUCANASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31044:SF25">
        <property type="entry name" value="PLASMODESMATA CALLOSE-BINDING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07983">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00768">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041466608" description="X8 domain-containing protein" evidence="11">
        <location>
            <begin position="20"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="183"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="X8" evidence="12">
        <location>
            <begin position="20"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="108"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9677577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="207" mass="21145" checksum="2C14E1DBF7D618D7" modified="2024-01-24" version="1">MAVLVYMVLILAMAGHSSANWCVCKEGLSDSVLQKTLDYACGAGADCGPIHQTGGCYNPNTVRAHCSYAVNSYFQKKGQAQGTCDFAGTASVATSDPSSSGCVYPSSLSSAGTSTTPSTTTPSTTPSTTTPSTTPSTTTPSTTTPSTTTPYTGTPSTGVYGGINNGVGPSGAGINTDISHGGFMLQNTCMFSFFITLWFSGLMLLWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YW99</accession>
    <name>A0AA38YW99_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_022650</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YW99"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000020">
        <property type="entry name" value="Transposon Tf2-6 polyprotein-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041577">
        <property type="entry name" value="RT_RNaseH_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17919">
        <property type="entry name" value="RT_RNaseH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9677794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="334" mass="38644" checksum="427B9B1D251E38AB" modified="2024-01-24" version="1">MSPCAVPVLLVPKKDRTWKICVDYRAINNITVKYRHSIPRLDDMLDELHGSCVFTKIDLKSGYHQIRMKEGDEWKTAFKIKYGLYEWLAMSFGLTNAPSTFMRLMNHALREFIGIFVVVYFDDILVYSRNLDEHINHLHCVLAVLRKEKLYANLKKCSFCMDKVVFLGYVVSAKGIEVDEEKVKAIKEWPTRKSITEVRSFHGLASFYRRFVKAFSTLAAPLTEIVKKSVGFKWSSEQDRAFIEIKERLCGAPLLALPYFSKTFEIEYDASGIVIGAVLMQEKRSTTYFSEKLNKATLNYPTYHKELYALVRALRLGNITFGRNNFLYITTMSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YX49</accession>
    <name>A0AA38YX49_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_022829</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9678059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YX49"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9678059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="234" mass="26248" checksum="93A944515B4D12FA" modified="2024-01-24" version="1">MSLYMWFIQKINVAYGHLLIKEGVDIPITCTHVEQVFRIPPGGRKLVLDNHYHRDGRVPSIQNIERLMVETENAKEFCQLFIIFTCTTVLASTMCLEGHHALWHAPPKVVTRDFHHALLNEGVENNGLTNDSNTVMEMGAGNESFQAIVAHMEATCRQIDATQAQMNNLIVAYGHNLITLKKLQPNPSIPIDEDFQPFENMADVAEPIVERPVDEAIVKGDDDVGPPNDVSPRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YYE3</accession>
    <name>A0AA38YYE3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ClpA/ClpB AAA lid domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_020975</name>
        <name evidence="8" type="ORF">PVL29_020979</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ClpA/ClpB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YYE3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000120">
        <property type="entry name" value="ATP-dependent chaperone ClpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041546">
        <property type="entry name" value="ClpA/ClpB_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050130">
        <property type="entry name" value="ClpA_ClpB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11638">
        <property type="entry name" value="ATP-DEPENDENT CLP PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11638:SF86">
        <property type="entry name" value="CHAPERONE PROTEIN CLPB4, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17871">
        <property type="entry name" value="AAA_lid_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ClpA/ClpB AAA lid" evidence="6">
        <location>
            <begin position="83"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="175"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF17871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9678930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9678934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="393" mass="44511" checksum="37DFC3B9742AAD26" modified="2024-01-24" version="1">MKEKQESRGQLLAQRIVRGDVPEPLMNRKLISLDMGSLLAGAKFRGDFEERLKAVLKEVTASNGQIILFIDEIHTVVGAGAPSVEDTISILRGLRERYELHHGVKISDSALVSAAVLADRYITERFLSDKAIDLVDEAAAKLKIEITSKPTELDEIDRAVIKLKMEKLSLKSDTDKASRERLSKLENDLLSLKQKQKDLTDQWEQEKVLMTRIRSIKEEIDRVNLEMESAEREYNLNRAAELKYGTLISLQRQLEEAEKNLPNYRKSGKSLLREEVTDLDIAEIVSKWTGIPLSNLQQSERDKLVLLEQVLHQRVVGQENAVKSVADAIRRSRAGLSDPNRPIASFMFMGPSEVISHYLSHSCAICMATFGHVSFCLCFIFDFKINSQFPIFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z0G4</accession>
    <name>A0AA38Z0G4_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_019399</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z0G4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025886">
        <property type="entry name" value="PP2-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14299">
        <property type="entry name" value="PP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9680100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="66" mass="7587" checksum="4C5221A9877462E1" modified="2024-01-24" version="1">MIRGEWIELNVGSFRMVKGETGEECFDLYKHGGHPENRELYFGLCEHGGHWKTGLIIKGVILRARN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z1R8</accession>
    <name>A0AA38Z1R8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">LOB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_019939</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LOB domain-containing protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z1R8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009755">
        <property type="term" value="P:hormone-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004883">
        <property type="entry name" value="LOB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31529">
        <property type="entry name" value="LOB DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31529:SF4">
        <property type="entry name" value="LOB DOMAIN-CONTAINING PROTEIN 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03195">
        <property type="entry name" value="LOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50891">
        <property type="entry name" value="LOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LOB" evidence="3">
        <location>
            <begin position="30"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="137"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="183"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="10"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="138"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="153"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="183"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="243"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9680776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="253" mass="26023" checksum="BC94B67C7C822968" modified="2024-01-24" version="1">MSANTSSSSGGVGGGGGGGGSTSGGGGGGGPCGACKFLRRKCVVGCIFAPYFDPEQGAAHFAAVHKVFGASNVSKLLGHIPVHKRLDAVVTICYEAQARLRDPVYGCVAHIFALQQQVVNLQAELSYLQAHLATLELPSPPPPPPQPPPVMVTPPSLSISDLPSASSMPPTYDLSSLFDPMVQPSSWPMQQHRQIDPRQFGGSRGPTDVPSTAGGSGGGDLQALARELLHRHSSALGGHVPCTESSMPPSISK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z3R7</accession>
    <name>A0AA38Z3R7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_017970</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9681856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z3R7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22162">
        <property type="entry name" value="F-box_AtSKIP3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025886">
        <property type="entry name" value="PP2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32278">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32278:SF15">
        <property type="entry name" value="F-BOX PROTEIN PP2-B13-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14299">
        <property type="entry name" value="PP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="7"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9681856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="285" mass="32638" checksum="2E2E07D655C2981D" modified="2024-01-24" version="1">MECLGFNKLPEGCVSTILSLTSPPDACKSSLVSPMFRAAAESDVVWERFLPADYHDILCRLVEPLVCSSRKELFLRLCDPILIDGGSKSFRLDKWSGKKSYLLSARELSITWSHDPMYWSWRSTPESRFSQVAELRTMCWLEIHGKMRTQMLSPNTKYGAYLIMKISNRAYGLDLMPSEISVEVSNSTQVCNGTAYLRCSDSKNKQMERLFYGNRREMLRSRVIEGDGRVPCEREDGWMELELGDFFNGEGDEEVRMSLREVKGYHLKGGLIIEGIEVRPKDSRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z5B2</accession>
    <name>A0AA38Z5B2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Retrotransposon Copia-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_018569</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z5B2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029472">
        <property type="entry name" value="Copia-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47481">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47481:SF35">
        <property type="entry name" value="ZINC FINGER, CCHC-TYPE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14244">
        <property type="entry name" value="Retrotran_gag_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="257"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="Retrotransposon Copia-like N-terminal" evidence="3">
        <location>
            <begin position="2"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="130"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="162"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9682671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="281" mass="31599" checksum="B45A969F40901DF0" modified="2024-01-24" version="1">MITIKLSPSNYLFWKSQLLSFLESQDLLGYVNGTLVPPPRFEPATSTTLSTKYLLMKRDTKLVAEYAHTFKTLCDQLHAIGRPVEDTNKVHWFLHGLGTDFFSFFYRSDGSHPSPLFELFQRSLESSDSTTAAFTTTNRGRTTSHGTPFASRGNQRGRSHSHGNNSSNQGRTHSGQGRQPPRCQICRMEDHYADRCNQRYARTDSFAHLAKAFNTSCSLSGPEVADWFLDTRASAHMTTDPSILDQSKNYMGKDSMIVGNSASLSLLPTLVLFLLFQIFTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z6B6</accession>
    <name>A0AA38Z6B6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PVL29_018714</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z6B6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000590">
        <property type="entry name" value="PR5-like receptor kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000178">
        <property type="entry name" value="serine/threonine-protein kinase PBS1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045874">
        <property type="entry name" value="LRK10/LRL21-25-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032872">
        <property type="entry name" value="WAK_assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025287">
        <property type="entry name" value="WAK_GUB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27009">
        <property type="entry name" value="RUST RESISTANCE KINASE LR10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13947">
        <property type="entry name" value="GUB_WAK_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14380">
        <property type="entry name" value="WAK_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 17" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041453414" description="non-specific serine/threonine protein kinase" evidence="17">
        <location>
            <begin position="26"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="261"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="328"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="356"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ9682842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="618" mass="69083" checksum="A64B1C5FC54EDBD4" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z767</accession>
    <name>A0AA38Z767_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">CRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_019188</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z767"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000373">
        <property type="term" value="P:Group II intron splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.110.60:FF:000003">
        <property type="entry name" value="CRM-domain containing factor CFM3B, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.110.60:FF:000002">
        <property type="entry name" value="CRS2-associated factor 1, chloroplastic"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.60">
        <property type="entry name" value="YhbY-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045278">
        <property type="entry name" value="CRS1/CFM2/CFM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001890">
        <property type="entry name" value="RNA-binding_CRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035920">
        <property type="entry name" value="YhbY-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31846:SF20">
        <property type="entry name" value="CRM-DOMAIN CONTAINING FACTOR CFM2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31846">
        <property type="entry name" value="CRS1 / YHBY (CRM) DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01985">
        <property type="entry name" value="CRS1_YhbY"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01103">
        <property type="entry name" value="CRS1_YhbY"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75471">
        <property type="entry name" value="YhbY-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51295">
        <property type="entry name" value="CRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="3" id="KW-0934">Plastid</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="CRM" evidence="13">
        <location>
            <begin position="160"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="CRM" evidence="13">
        <location>
            <begin position="379"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="domain" description="CRM" evidence="13">
        <location>
            <begin position="580"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="262"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="510"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="542"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="17"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="513"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9683507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="704" mass="79506" checksum="3C951D8D6EC61B10" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z8K2</accession>
    <name>A0AA38Z8K2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phospholipid/glycerol acyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_016675</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GPAT/DAPAT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z8K2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090447">
        <property type="term" value="F:glycerol-3-phosphate 2-O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010143">
        <property type="term" value="P:cutin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06551">
        <property type="entry name" value="LPLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056462">
        <property type="entry name" value="HAD_RAM2/GPAT1-8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15486">
        <property type="entry name" value="ANCIENT UBIQUITOUS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15486:SF54">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23270">
        <property type="entry name" value="HAD_RAM2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="222"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="9">
        <location>
            <begin position="279"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9684304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="487" mass="54684" checksum="54AC4D3405F977C8" modified="2024-01-24" version="1">MESVVSELEGTLVKDPDPFSYFMLVAFEASGLVRFALLLILWPIIRLLDMFGMGDAGLRLAIFVAFAGVHESEIESVARAVLPKFYMDDLDMEAWKVFSSYDRRVVVTKTPRVMVERFLKEHLRADEVIGSELVVNRFGFATGFTRNEAGSIADRVSMLFEDNQPDMGLGRIPSGSSFLSLCREQSHPPFMTNQDRDRQLLRPLPVIFHDGRLVKRPLPSTALLIILWIPFGILLAITRIAMGLLLPMWLIPYVSRLFGGKIIVKGKPPPPASGSNSGVLFVCTHRTLMDPVVLSTVLQRSIPAVTYSISRFSEILSPIPTVRLTRIRHVDAEKIKRELAKGDLAVCPEGTTCREPFLLRFSALFAELTDRIVPVAMNYRVGFFHATTARGWKGLDPIFFFMNPRPVYEVTFLNQLPVEATCSAGKSPHDVANYVQRILAATLGFECTNFTRKDKYRVLAGNDGIVSYTSFLDQMKKVVTNFNPFIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z990</accession>
    <name>A0AA38Z990_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Tetraspanin-6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_016847</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z990"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044991">
        <property type="entry name" value="TET_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32191">
        <property type="entry name" value="TETRASPANIN-8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="69"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="220"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9684575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="287" mass="32662" checksum="CB16066E00E986EA" modified="2024-01-24" version="1">MYRFSNTVIGFLNLFTLLASIPIIGGGLWMARSSTTCENFLQTPLLVVGFVVLIISLAGFIGACFNVAWALWLYLVVMLFLIGTLLGLTIFGFVVTSQGGGVAVPGRVYKEYQLEKYSPWLKSRIKDPHYWSTIRSCILGSKTCAQIAYWTPLDYLERDMTPIQSGCCKPPTSCNYATATMMTQDADCYRWNNAPNLLCYECDSCKAGVLEHIRRDWHKLSVLNIVMLVLLIGIYSIGCCAFRNTRRSETDYPYGENRMSKVRPRWDFYWYVTFILDPTRYPLLGQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZA52</accession>
    <name>A0AA38ZA52_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_017322</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZA52"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="10"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9685245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="99" mass="11113" checksum="5D8BA7DE7E73BBD2" modified="2024-01-24" version="1">MQSNPPSPVAPSSSLVAAPRKTKGRGSRDDTNADCNSRYAARDFDSLDCDSVDFFHVQPCENGRQRRLVLTSDSMEKESHVFLIDHTWTFRLSDAPKQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZB18</accession>
    <name>A0AA38ZB18_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_017332</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZB18"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="247"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9685258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="307" mass="35068" checksum="5F58F6D686AF9603" modified="2024-01-24" version="1">MAPKRAPAEASSNASKGKRPKTSGNKQKTSEVVKSSTNKGKRAVVDENVEVCKKATFDRATFTTPELAQRFNLHFANRTVILGRNIDFSKINYFQFDRLFTRMGWLPIVSVKEFLYPRWPRVEGFKPAEAVHRLCGYQKSSRPTSHSLTVLSCILQHMISYIFIPKGGHRDDVSFLEAFLVDSILTKRKINTGYIIFRQMKTCSLSEDLIVKYFNVNLQNEIDGKKLKSFDTYDWASLYRMHFSSVPPSKVDVSSNDGSSQEEEYKNQDVERRGYENANVIAIPSNDETRLRHRITSMPRLAPLVPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZCF0</accession>
    <name>A0AA38ZCF0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_015064</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9685992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZCF0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045274">
        <property type="entry name" value="WAK-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27005:SF466">
        <property type="entry name" value="NON-FUNCTIONAL PSEUDOKINASE ZED1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27005">
        <property type="entry name" value="WALL-ASSOCIATED RECEPTOR KINASE-LIKE 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="46"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9685992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="365" mass="41601" checksum="DA9F6C5D927ED67D" modified="2024-01-24" version="1">MAKRRDELFLKNGSLLLEKLVCSFNGKSNPIRSFSDKELQKATNNYSSALDLHDSGYYKCYKGSVDDRLICVRKFDPNYRYPFEGPLERIINEIAIATQMSAHKNVLKLLGCCLETQFPTLVYEFPMDGSLSHLSPNPNRRIKSQPLLLKSRLRIAWEIANVAAYLHTAFARPFIHRDIRLDNFFLDQDCAPKLFNFFFCIPIPEGETHATDEVMGITGNIAPESLETGKFTEKNDVFSFGTLLLELLTGQRGYDLSQDETALLLNCGDGVVDATIPVEGEGILEQQVYKLFLRCSSLTAEERPTMVDVSKELRQILRLGSEDNLRSKSASGMSLPAHLQQQPWQRARRLPHWYLLAEKMRENRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZDX6</accession>
    <name>A0AA38ZDX6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycosyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_015935</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="5"/>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZDX6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035251">
        <property type="term" value="F:UDP-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000056">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000080">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050481">
        <property type="entry name" value="UDP-glycosyltransf_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48048">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48048:SF45">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9687258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="468" mass="51889" checksum="5292F3407FA5226B" modified="2024-01-24" version="1">MKQTELIFIPLPIIGHLSPTVEIAKLLTQRDPRFSITIFITKFPFGSIDSMTKDSDSIRFVTLPAVEISSGATRPGPLMSEFIKAQTLLVRDAVHELTRSNSVRLAGFVIDALCTHMIDVADEFGVPSYLFSTSSAASLGFLLHLQFLHDYEGLNLDEFKDSDAELQVPSYANSVPGKVFPSLIFDKEVGGSAGYMYHMRRLRQAKGVMVNTFIDLESHAIQSFSGSKIPPVYPVGPILNTQMGYGGDQQDASAIMSWLDDQPPSSVVFLCFGSIGSFGVDQIKEIAYGLERSGHRFLWSLRQAPPNGKMAYPRDYENIEEVLPEGFLHRTARIGKVIGWAPQVAVLAHSAVGGFVSHCGWNSLLESIWYGVPVATWPMYAEQQINAFQMVKDLGLAVEIKIDYNKDNNYIVNAHEIENGIKNLMSINSEVRKKMNEMQQISRRVMIDGGSSHSSLGHFIENVMTNIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZED4</accession>
    <name>A0AA38ZED4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NADH:ubiquinone reductase (non-electrogenic)</fullName>
            <ecNumber evidence="5">1.6.5.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PVL29_015621</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a quinone + NADH + H(+) = a quinol + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:46160"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.6.5.9"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a ubiquinone + NADH + H(+) = a ubiquinol + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:23152"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Intermembrane side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the NADH dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.6.5.9" evidence="5"/>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZED4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050136">
        <property type="term" value="F:NADH dehydrogenase (quinone) (non-electrogenic) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.100:FF:000008">
        <property type="entry name" value="External alternative NAD(P)H-ubiquinone oxidoreductase B1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.100">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045024">
        <property type="entry name" value="NDH-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054585">
        <property type="entry name" value="NDH2-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43706:SF47">
        <property type="entry name" value="EXTERNAL NADH-UBIQUINONE OXIDOREDUCTASE 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43706">
        <property type="entry name" value="NADH DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22366">
        <property type="entry name" value="NDH2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-0520">NAD</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="EF-hand" evidence="17">
        <location>
            <begin position="403"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ9686849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="603" mass="68105" checksum="840F6E542BD1BE9D" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZF68</accession>
    <name>A0AA38ZF68_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MOB kinase activator-like 1A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_013936</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9687951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZF68"/>
    <dbReference type="SMR" id="A0AA38ZF68"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.30:FF:000001">
        <property type="entry name" value="MOB kinase activator 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.30">
        <property type="entry name" value="MOB kinase activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005301">
        <property type="entry name" value="MOB_kinase_act_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036703">
        <property type="entry name" value="MOB_kinase_act_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22599">
        <property type="entry name" value="MPS ONE BINDER KINASE ACTIVATOR-LIKE MOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03637">
        <property type="entry name" value="Mob1_phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01388">
        <property type="entry name" value="Mob1_phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101152">
        <property type="entry name" value="Mob1/phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9687951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="215" mass="24607" checksum="3E57237018B8C95F" modified="2024-01-24" version="1">MSLFGLGSRNQKTFRPKKSAPSGSKGAQLQKHIDATLGSGNLREAVRLPPGEDPNEWLAVNTVDFFNQVNILYGTLTEFCTASICPTMSAGPKYEYRWADGVTIKKPIEVSAPKYVEYLMDWIETQLDDESIFPQKLGAPFPNNFQDVVKTIFKRLFRVYAHIYHSHFQKIVSLKEEAHLNTCFKHFVLFTWEFRLIDKGELAPLYDLIESILQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZFH4</accession>
    <name>A0AA38ZFH4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Survival protein SurE-like phosphatase/nucleotidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_014049</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SurE nucleotidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZFH4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008252">
        <property type="term" value="F:nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1210.10">
        <property type="entry name" value="Survival protein SurE-like phosphatase/nucleotidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030048">
        <property type="entry name" value="SurE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002828">
        <property type="entry name" value="SurE-like_Pase/nucleotidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036523">
        <property type="entry name" value="SurE-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30457">
        <property type="entry name" value="5'-NUCLEOTIDASE SURE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30457:SF5">
        <property type="entry name" value="OS01G0709400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01975">
        <property type="entry name" value="SurE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64167">
        <property type="entry name" value="SurE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Survival protein SurE-like phosphatase/nucleotidase" evidence="5">
        <location>
            <begin position="116"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9688113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="443" mass="48004" checksum="2D30DC924E2AAFBC" modified="2024-01-24" version="1">MLAQSCHVNKQPVQEPNRTAQPKPEISGAHCEYFHLYPFSVLLEQAILPSSSFSSLLFSSFSSFSLLVKKKSMEPASLISNLQQVLITKREDGNRISSCSSSVDESAVKDCSRAVVLVTNGDGIESPGLTSLVQTLLREPRFHVHVCAPQSDKSVSGHSMTVQETLTACSAEIGGATAYEVSGTPADCVSLALSGALFSWSKPVLVICGINKGSSYGLNMFHSGAVAGAREALLCGVPALCISLNWKEDVSCDNDFKDAVNVCLPIIHAAIRDIEKGIFPKSCLVNIEIPSSPLKNKGFKVARQSLRRPALIWQAVSANKHPSIGHFMSNQQSLGFKLAQLGRDASAVGAARRLNSDQKNQEIESVGVAGKLSSQKTVKKYFRLKFQEMEQGKADEDLDFKAVENGFVAITPHSLSSYSLPGVQTWILNWINFHFPENMLLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZFN4</accession>
    <name>A0AA38ZFN4_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_014096</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZFN4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35505">
        <property type="entry name" value="OS01G0600300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35505:SF5">
        <property type="entry name" value="SUBSTRATE CARRIER FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="478"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="478"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="506"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="534"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9688203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="545" mass="61100" checksum="14F88F2601CFED38" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZGD2</accession>
    <name>A0AA38ZGD2_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_014207</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZGD2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000324">
        <property type="entry name" value="Transcription factor MYB3R-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000010">
        <property type="entry name" value="Transcriptional activator Myb isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000016">
        <property type="entry name" value="Transcriptional activator Myb isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF266">
        <property type="entry name" value="TRANSCRIPTION FACTOR MYB3R-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="40"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="40"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="92"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="92"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="144"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="148"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="526"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="809"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="968"/>
            <end position="1050"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="531"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="824"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="977"/>
            <end position="987"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="992"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1037"/>
            <end position="1050"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9688412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1050" mass="114962" checksum="0DBAACEADA1FD454" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZIJ6</accession>
    <name>A0AA38ZIJ6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Dicer-like protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PVL29_014506</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="12 13">Belongs to the helicase family. Dicer subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030422">
        <property type="term" value="P:siRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18034">
        <property type="entry name" value="DEXHc_dicer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00593">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1520.10:FF:000031">
        <property type="entry name" value="Dicer-like protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000628">
        <property type="entry name" value="Endoribonuclease Dicer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1520.10:FF:000004">
        <property type="entry name" value="Endoribonuclease dicer-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.380:FF:000001">
        <property type="entry name" value="Endoribonuclease dicer-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.380">
        <property type="entry name" value="Dicer dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.260.10">
        <property type="entry name" value="paz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1520.10">
        <property type="entry name" value="Ribonuclease III domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038248">
        <property type="entry name" value="Dicer_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005034">
        <property type="entry name" value="Dicer_dimerisation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003100">
        <property type="entry name" value="PAZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000999">
        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036389">
        <property type="entry name" value="RNase_III_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14950:SF15">
        <property type="entry name" value="DICER-LIKE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14950">
        <property type="entry name" value="DICER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03368">
        <property type="entry name" value="Dicer_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14709">
        <property type="entry name" value="DND1_DSRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00636">
        <property type="entry name" value="Ribonuclease_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00358">
        <property type="entry name" value="DSRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00949">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00535">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69065">
        <property type="entry name" value="RNase III domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51327">
        <property type="entry name" value="DICER_DSRBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50137">
        <property type="entry name" value="DS_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50821">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00517">
        <property type="entry name" value="RNASE_3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50142">
        <property type="entry name" value="RNASE_3_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="8" id="KW-0347">Helicase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 13" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="17">
        <location>
            <begin position="53"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="18">
        <location>
            <begin position="252"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="Dicer dsRNA-binding fold" evidence="19">
        <location>
            <begin position="443"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="domain" description="PAZ" evidence="16">
        <location>
            <begin position="713"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="domain" description="RNase III" evidence="15">
        <location>
            <begin position="850"/>
            <end position="1025"/>
        </location>
    </feature>
    <feature type="domain" description="RNase III" evidence="15">
        <location>
            <begin position="1066"/>
            <end position="1210"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="14">
        <location>
            <begin position="1236"/>
            <end position="1302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00035116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAJ9688889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="22">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1489" mass="168206" checksum="694320A566580C65" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZJR7</accession>
    <name>A0AA38ZJR7_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_012520</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9689892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZJR7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044851">
        <property type="entry name" value="Wax_synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31595:SF77">
        <property type="entry name" value="ACYL-COA--STEROL O-ACYLTRANSFERASE 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31595">
        <property type="entry name" value="LONG-CHAIN-ALCOHOL O-FATTY-ACYLTRANSFERASE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="225"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9689892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="285" mass="31762" checksum="2E2F6A13BF33AA5E" modified="2024-01-24" version="1">MEGKFNNFVMVWVSAFVSLSYCYTMAKIIPNGTTRLLTIIPVVALFLFLPLRLHTLHLSGTSAFFIAWLANFKLLLFAFRQRPFIIRPMNLLPPFRSPSLENPSTEKPKKGQKSPLVYAVKGAVLALVIVAVHFRENMHPIVSLFLLLFQMYLSLEFILALVASLQPSLEFFWAWSSLHSSTNPTSPPPSKTSGEDDGTSWLPVSCAPVYEPTMLLKQVLAGAPALPAFVSGPLAVAFVVATGFWLFFPQLFRLNADVRGVEEYAAFVTFLNNIGRALTFNLIRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZKB4</accession>
    <name>A0AA38ZKB4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CLAVATA3/ESR (CLE)-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_013007</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZKB4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041250067" description="CLAVATA3/ESR (CLE)-related protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9690630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="81" mass="9425" checksum="104C193C0A2BAFA7" modified="2024-01-24" version="1">MASLRFWLCLILVVLSFVRTEPQPLSPFIEGNGRKSLDAFIEQAKEEFKERLRREDANRNMYKLKRQSPGGPDPKHHSKNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZKQ1</accession>
    <name>A0AA38ZKQ1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_012689</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZKQ1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010333">
        <property type="term" value="F:terpene synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016102">
        <property type="term" value="P:diterpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00684">
        <property type="entry name" value="Terpene_cyclase_plant_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.600.10:FF:000007">
        <property type="entry name" value="Isoprene synthase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.130">
        <property type="entry name" value="Terpene synthase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034741">
        <property type="entry name" value="Terpene_cyclase-like_1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044814">
        <property type="entry name" value="Terpene_cyclase_plant_C1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001906">
        <property type="entry name" value="Terpene_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036965">
        <property type="entry name" value="Terpene_synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050148">
        <property type="entry name" value="Terpene_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005630">
        <property type="entry name" value="Terpene_synthase_metal-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225">
        <property type="entry name" value="OS04G0344100 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225:SF234">
        <property type="entry name" value="TERPENE SYNTHASE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01397">
        <property type="entry name" value="Terpene_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03936">
        <property type="entry name" value="Terpene_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01019">
        <property type="entry name" value="Terpene_Cyclase_Like_1_C_Termi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Terpene synthase N-terminal" evidence="3">
        <location>
            <begin position="31"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="Terpene synthase metal-binding" evidence="4">
        <location>
            <begin position="250"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9690163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="549" mass="62833" checksum="3E3D1C5A2E34876E" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZLC9</accession>
    <name>A0AA38ZLC9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_012886</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZLC9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021319">
        <property type="entry name" value="DUF2921"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057425">
        <property type="entry name" value="DUF2921_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33389">
        <property type="entry name" value="FAMILY PROTEIN, PUTATIVE (DUF2921)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33389:SF22">
        <property type="entry name" value="FAMILY PROTEIN, PUTATIVE (DUF2921)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11145">
        <property type="entry name" value="DUF2921"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25333">
        <property type="entry name" value="DUF2921_N"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="669"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="702"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="746"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="786"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="910"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2921" evidence="12">
        <location>
            <begin position="60"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2921" evidence="12">
        <location>
            <begin position="291"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2921" evidence="12">
        <location>
            <begin position="464"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="domain" description="SWEET-like" evidence="11">
        <location>
            <begin position="658"/>
            <end position="943"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF11145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF25333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9690463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="960" mass="107031" checksum="ED439DF0F837C4F8" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZP95</accession>
    <name>A0AA38ZP95_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7795 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_011707</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZP95"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056697">
        <property type="entry name" value="DUF7795"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35305">
        <property type="entry name" value="FAD-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35305:SF2">
        <property type="entry name" value="FAD-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25071">
        <property type="entry name" value="DUF7795"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7795" evidence="2">
        <location>
            <begin position="65"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9692746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="287" mass="32918" checksum="11AC0D21646D8157" modified="2024-01-24" version="1">MGKNPRLHRGPSFPSARANKQVPSTDSVYFDFRYVPINLRLQLTGSGASIWLRMEDEECNLLIEPRQRIVQKFSDFMTRVAEFEELAAVGSRLLIGFQQGLEFLRRPPINKTSELVEKIIKSNETKRVKSYVEAGCIDSHDGTQNISKLHTCQLGLQDHLSKAKCILSEIESLIDDVVGGMQTTNENLSDLQDRYFDKWDQQEPAYDKEEITSTHASHLQKHEAIDYAAMMGIIFSMVKQDYVMQERIVSSLNLKSLAGELESYCLMWSLRPFINDEIMHQAWKLLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZPD5</accession>
    <name>A0AA38ZPD5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PGG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_011774</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZPD5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026961">
        <property type="entry name" value="PGG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24186:SF56">
        <property type="entry name" value="PGG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24186">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13962">
        <property type="entry name" value="PGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="PGG" evidence="7">
        <location>
            <begin position="29"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9692841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="167" mass="18133" checksum="B0F5DEAAD3F11397" modified="2024-01-24" version="1">MATQLNPPEDASDNFYAYYRFNPSRDNPDSARNTLLVVGALIAAATFQAGINPPGGVWQDKTTVDGVTTHPGKAIFGSEKAAFTVFLFCNTLAFSSSLQMITYLIIGCPFSLEVLTGIYCMSGTYGASIAAVEPPGAVKSEYILLAFFLPYAVRMSQQIFKKYKKRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZT05</accession>
    <name>A0AA38ZT05_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Integral membrane bound transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_010247</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZT05"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049453">
        <property type="entry name" value="Memb_transporter_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30509:SF34">
        <property type="entry name" value="F3L24.34 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30509">
        <property type="entry name" value="P-HYDROXYBENZOIC ACID EFFLUX PUMP SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13515">
        <property type="entry name" value="FUSC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="44"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="100"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="130"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="152"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="411"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="437"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="470"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="505"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="534"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="domain" description="Integral membrane bound transporter" evidence="8">
        <location>
            <begin position="423"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="366"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9694681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="823" mass="90971" checksum="960FF91D3D9AE192" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZVH8</accession>
    <name>A0AA38ZVH8_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_008359</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZVH8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9696066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="126" mass="13803" checksum="45747F91369B70D7" modified="2024-01-24" version="1">MAAMDIKRDDNPNNSFLGKSLPKSFSWCLDVRMARNMTTAVTEKHRAMRDAMEKTLKAGWFSRVGTPSPSLTPPGTVADVANATVAMRAATTEHELEVSLSHSPPFSTRSAWLFVNISLAVLLLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZVK9</accession>
    <name>A0AA38ZVK9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Chlorophyllase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_008385</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZVK9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047746">
        <property type="term" value="F:chlorophyllase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015996">
        <property type="term" value="P:chlorophyll catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000582">
        <property type="entry name" value="Chlorophyllase-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017395">
        <property type="entry name" value="Chlorophyllase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33428:SF10">
        <property type="entry name" value="CHLOROPHYLLASE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33428">
        <property type="entry name" value="CHLOROPHYLLASE-2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07224">
        <property type="entry name" value="Chlorophyllase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9696111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="315" mass="33451" checksum="4EB03A6571FA2082" modified="2024-01-24" version="1">MAALEARPAPATATDVFKRGKYTAKPITTCNAPKTLFIVTPDTEGTYPVLLFLHGYNICPCCYTNLLEHISSHGYIVVAPRLLSLCSLYGRPDINSAAEVTNWLSSGLQPVLPENVVPDLSRLALAGHSRGGYLAFALALGNANVSMNLKFLTLIGIDLVAGVNKCMKMCPKILTGVPHSFNLDIPVMVIGTGLGGESVIGCIPCSCAPDSLNYAEFFNECKDNCLGFVIPDYGHMDMLDDDYCTNCIGTSIGAIMGSMCKSGKGDKTSMMECVGGLVVAMLMAHLEGETGDLDAIVDEPGIAPVKLEVMKDSEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZWP8</accession>
    <name>A0AA38ZWP8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Hexosyltransferase</fullName>
            <ecNumber evidence="7">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_008505</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycan metabolism; pectin biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the glycosyltransferase 8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="7"/>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZWP8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002495">
        <property type="entry name" value="Glyco_trans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050748">
        <property type="entry name" value="Glycosyltrans_8_dom-fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13778">
        <property type="entry name" value="GLYCOSYLTRANSFERASE 8 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13778:SF5">
        <property type="entry name" value="HEXOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01501">
        <property type="entry name" value="Glyco_transf_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041441295" description="Hexosyltransferase" evidence="8">
        <location>
            <begin position="31"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9696305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="344" mass="39113" checksum="10186D8BDADFC378" modified="2024-01-24" version="1">MAPWSPPSSLPLLGLLSIIIFLRPTSVVTSSSGIRHGIIRKPSSDEPAVVLFREAPAFRNGDACGSSDADRIHVVMTLDANYLRGTMAALLSILQHSTCPENIDFHFLWSHFESDIFSSINSTFPFLNFKVYRFDSNRVRGKISKSIRRALDQPLNYARIYLGDILPADVRRVIYLDSDLVMVDDIANLWGYCHANFTNYFTSAFWSDRALARTFDGRKPCYFNTGVMVVDVEKWREGGYTKKVEEWMAVQKQKRIYQLGSLPPFLLVLAGNMQPVDHRWNQHGLGGDNLEGRCRSLHPGPISLLHWSGKGKPWLRLDSRRPCTVDHLWAPYDLYRSSTSSLEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZX35</accession>
    <name>A0AA38ZX35_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Thiamine pyrophosphokinase</fullName>
            <ecNumber evidence="11">2.7.6.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_008621</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate (TPP). TPP is an active cofactor for enzymes involved in glycolysis and energy production. Plant leaves require high levels of TPP for photosynthesis and carbohydrate metabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>thiamine + ATP = thiamine diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11576"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18385"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:11577"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Cofactor biosynthesis; thiamine diphosphate biosynthesis; thiamine diphosphate from thiamine: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the thiamine pyrophosphokinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.2" evidence="11"/>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZX35"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030975">
        <property type="term" value="F:thiamine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004788">
        <property type="term" value="F:thiamine diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006772">
        <property type="term" value="P:thiamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07995">
        <property type="entry name" value="TPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.320:FF:000001">
        <property type="entry name" value="Thiamine pyrophosphokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10240:FF:000001">
        <property type="entry name" value="Thiamine pyrophosphokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10240">
        <property type="entry name" value="Thiamin pyrophosphokinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.320">
        <property type="entry name" value="Thiamin pyrophosphokinase, thiamin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006282">
        <property type="entry name" value="Thi_PPkinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016966">
        <property type="entry name" value="Thiamin_pyrophosphokinase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007373">
        <property type="entry name" value="Thiamin_PyroPKinase_B1-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036371">
        <property type="entry name" value="TPK_B1-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007371">
        <property type="entry name" value="TPK_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036759">
        <property type="entry name" value="TPK_catalytic_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01378">
        <property type="entry name" value="thi_PPkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13622">
        <property type="entry name" value="THIAMIN PYROPHOSPHOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13622:SF8">
        <property type="entry name" value="THIAMIN PYROPHOSPHOKINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04265">
        <property type="entry name" value="TPK_B1_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04263">
        <property type="entry name" value="TPK_catalytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF031057">
        <property type="entry name" value="Thiamin_pyrophosphokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00983">
        <property type="entry name" value="TPK_B1_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63999">
        <property type="entry name" value="Thiamin pyrophosphokinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63862">
        <property type="entry name" value="Thiamin pyrophosphokinase, substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiamin pyrophosphokinase thiamin-binding" evidence="12">
        <location>
            <begin position="181"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00052367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR031057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9696485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="256" mass="28431" checksum="95E6ACF951B8C87D" modified="2024-01-24" version="1">MDLMRHSSAFLLPSIPDDGPPPTYALVVLNQRLPRFTPLVWKHAQLRLCADGGANRVYDEMPGLLADEDGNDVRNRYKPDAVKGDMDSVRTEVLEFYSNLGTKIVDESHDQDTTDLHKCIAFIRDFTPDLDKSNLCILVAGALGGRFDHEVGNINVLYRFSTMRIILLSDDCLIHLLPRTHHHEIHIQSSVEGPHCGLVPIGTPSGSTTTTGLQWDLNDTEMKFGGLISTSNIVKGDKITVQSDSDLLWTISIKKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZXB8</accession>
    <name>A0AA38ZXB8_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_008701</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZXB8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010433">
        <property type="entry name" value="EIF-4B_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32091:SF24">
        <property type="entry name" value="DUF4005 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32091">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06273">
        <property type="entry name" value="eIF-4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="294"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="333"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="396"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="357"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9696620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="450" mass="51917" checksum="83842E5613FD36AC" modified="2024-01-24" version="1">MVGGETKIESAFSWADEVEKEEEEARIRTHERQRPNPFGSARPREVVLQEKGIDWRRLDQDLHQPSNTRKQPAYSARAANKKQEISSGRSLQSKSEIRDPGFNRREVSGGFMTPKKQMGGVFVGPPPRYQTRNVLAYLSEEAYHNSYRLDSEERKFQYRNHIDFLYKNSNEAGREMMFHEPQTKQFYNSSGLGIEGHIHHYDDQFGPHNSLVGWEREYREFDFGGRRYSGVELRRPWMEALHCGRNLGKSVACEERRDADADYNRRKFMNHSSGCWPLVNERREALLIHEKRPPSVDDHARNGGVGRRLMNPPSYGAAQQRREALLIHEKRPPYVDHAGNGGVGRRLMNPPSNGAAQQRQQQQQRQHNNSGSRMVKLTKREMYLEKMENETEWQGDAGRFAGGSMEEAGDGERAEGQCNTKKKGANSAGNYRKRMMKSGPESYHQHGKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZXU7</accession>
    <name>A0AA38ZXU7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Trichome birefringence-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_009208</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PC-esterase family. TBL subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZXU7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016413">
        <property type="term" value="F:O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029962">
        <property type="entry name" value="TBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026057">
        <property type="entry name" value="TBL_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285:SF36">
        <property type="entry name" value="PROTEIN TRICHOME BIREFRINGENCE-LIKE 38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285">
        <property type="entry name" value="PROTEIN TRICHOME BIREFRINGENCE-LIKE 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13839">
        <property type="entry name" value="PC-Esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Trichome birefringence-like C-terminal" evidence="2">
        <location>
            <begin position="21"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9697308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="80" mass="9420" checksum="D588983FC86D7F72" modified="2024-01-24" version="1">MVATSSNHSKILVIHEANHECDYDLSVMLFHSQYLVDIENEQNGRILKLDSLKNGEIWKNIDVLVFNTWLWWYRAGLKQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZY40</accession>
    <name>A0AA38ZY40_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_008555</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZY40"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.80">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003441">
        <property type="entry name" value="NAC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036093">
        <property type="entry name" value="NAC_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31989">
        <property type="entry name" value="NAC DOMAIN-CONTAINING PROTEIN 82-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02365">
        <property type="entry name" value="NAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101941">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51005">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NAC" evidence="7">
        <location>
            <begin position="7"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="191"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9696389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="288" mass="33507" checksum="D6C6AC9AA7D8F4A3" modified="2024-01-24" version="1">MDHVGEGESASVFKPTDEEMVHYLLNKVTGKTPFVREVIECDLYGDEELWRKRVEGTQRNCLYFFTKLKKTTQKMSHSSRATAWRNTWKAQSDKKICMGEKVHIGSKRCFSLVEKEKPREKLGWVMHEYRLDGVYDWYNQGNDYVICRIKRMVSKKVAVKQQEVEGDDAIAIQTNCDEILDPKVTVLKDQETERLGSSTPQREDDFFGQQEETPPRGVDFAEGGGGNISWIQTFAQDLLAYSQNCVEEKDEGVDMLPNHFYQDRWIRGWERAPTDQMAKFVHHSLDFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A207</accession>
    <name>A0AA39A207_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ketoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_007536</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the short-chain dehydrogenases/reductases (SDR) family. SDR65C subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A207"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045000">
        <property type="entry name" value="TR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42898:SF6">
        <property type="entry name" value="NADP-DEPENDENT MANNITOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42898">
        <property type="entry name" value="TROPINONE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00822">
        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ketoreductase" evidence="4">
        <location>
            <begin position="18"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9698503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="269" mass="28809" checksum="CBD4AA467C8B5071" modified="2024-01-24" version="1">MAQTGCSNGDNRWSLKGMTALVTGGTRGIGHAVVEELAGLGARIHTCSRTETELNEYLREWEGKGFEVTGSVCDVSSRAQREKLMETVSSKFNGKLNILINNAGTGKPRRTVEFTAEEFSSIMAVNFESVYHLCQLAHPLLKASGAGSIVLMSSVAGVVSLKYLSAYGATKGALNQLAKSLACEWAQDNIRANSIAPWFIKTSLVEPSLSEKSFTEEIIRRTPLGRVGDPKEVSSLVAFLCLPASSYITGQTVCVDGGMSVNGFDPSQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A216</accession>
    <name>A0AA39A216_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cyclin-dependent kinases regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_008099</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Binds to the catalytic subunit of the cyclin dependent kinases and is essential for their biological function.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the CKS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9699334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A216"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.170.10:FF:000003">
        <property type="entry name" value="Cyclin-dependent kinases regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.170.10">
        <property type="entry name" value="Cyclin-dependent kinase, regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000789">
        <property type="entry name" value="Cyclin-dep_kinase_reg-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036858">
        <property type="entry name" value="Cyclin-dep_kinase_reg-sub_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23415">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASES REGULATORY SUBUNIT/60S RIBOSOME SUBUNIT BIOGENESIS PROTEIN NIP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01111">
        <property type="entry name" value="CKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00296">
        <property type="entry name" value="CYCLINKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01084">
        <property type="entry name" value="CKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55637">
        <property type="entry name" value="Cell cycle regulatory proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00944">
        <property type="entry name" value="CKS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2 4" id="KW-0132">Cell division</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU311113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9699334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="88" mass="10607" checksum="86441ACEFC954917" modified="2024-01-24" version="1">MGQIQYSEKYFDDTYEYRHVVLPPEVAKLLQKNRLLSENEWRAIGVQQSRGWVHYAIHRPEPHIMLFRRPLNYQQQQENQAQQGLLAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A353</accession>
    <name>A0AA39A353_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Acyl-CoA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_005769</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A353"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056819">
        <property type="entry name" value="ACBP4-6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093">
        <property type="entry name" value="ACYL-COA-BINDING DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093:SF4">
        <property type="entry name" value="GALACTOSE OXIDASE_KELCH REPEAT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24922">
        <property type="entry name" value="ACBP4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Acyl-CoA-binding" evidence="5">
        <location>
            <begin position="647"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="18"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="401"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="442"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="596"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="53"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="409"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF24922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9700098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="717" mass="78998" checksum="86283761F3CB277D" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A500</accession>
    <name>A0AA39A500_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Auxin-responsive protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_005704</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Aux/IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimers and heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Aux/IAA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9699989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A500"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033389">
        <property type="entry name" value="AUX/IAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003311">
        <property type="entry name" value="AUX_IAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31734:SF248">
        <property type="entry name" value="AUXIN-RESPONSIVE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31734">
        <property type="entry name" value="AUXIN-RESPONSIVE PROTEIN IAA17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02309">
        <property type="entry name" value="AUX_IAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0927">Auxin signaling pathway</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0678">Repressor</keyword>
    <keyword evidence="5 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="PB1" evidence="10">
        <location>
            <begin position="108"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="10"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="67"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9699989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="201" mass="21672" checksum="1DC968466785CB7B" modified="2024-01-24" version="1">MGGGAATPHSSSSSSSSSSSSSSSIDSISNNHPSLSSASSSISLPRNTKRSGLSTDLRLGPSISTAHIHNCSSSAPSPRDQRVDWPPIKPLLRSTLTGKADNQRQATNLFVKVYMEGISIGRKLDLFAHSGYDGLVTTLSHMFKTTIFCSDPHVGGADHSGNYHILTYEDKEGDWMMVGDVPWEMFLTTVKRLKITRADRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A6U5</accession>
    <name>A0AA39A6U5_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_006756</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A6U5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027850">
        <property type="entry name" value="DUF4504"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31366">
        <property type="entry name" value="UPF0739 PROTEIN C1ORF74"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31366:SF2">
        <property type="entry name" value="UPF0739 PROTEIN C1ORF74"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14953">
        <property type="entry name" value="DUF4504"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9701532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="304" mass="34185" checksum="76427E0FDE45A54A" modified="2024-01-24" version="1">METAKLEGVLHVLSTSLSRTKWRLKASSKRRLEIDILALCTGMRPVVMVDYGGKMPELQERLCAVLKLSQKASSIFYHLRIMVIEDMIYLIHVKELAEHVRSSLNSEPELHFVDLEHNPPKMIVQAEKSSVAMQLISVQKWFSLLFPVDEINNDLLPSITTEVMSIAKPSISEPITSQSSEIIDLSSGIQGTQVAVPTLNGWLLGYPVVYLFSTEHISDAIFNLSTKSLRIFKILICRNDAYKGSGKEELLSFSVPYDLSMGGSNEPWAKAFMAHLEAKWEKCKQVWSSLQMEVSEYPPQAIAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A702</accession>
    <name>A0AA39A702_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamyl-tRNA(Gln) amidotransferase subunit C, chloroplastic/mitochondrial</fullName>
            <shortName evidence="1">Glu-AdT subunit C</shortName>
            <ecNumber evidence="1">6.3.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">GATC</name>
        <name evidence="2" type="ORF">PVL29_003913</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in chloroplasts and mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-glutamyl-tRNA(Gln) + L-glutamine + ATP + H2O = L-glutaminyl-tRNA(Gln) + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Subunit of the heterotrimeric GatCAB amidotransferase (AdT) complex, composed of A, B and C subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GatC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.-" evidence="1"/>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9701899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A702"/>
    <dbReference type="SMR" id="A0AA39A702"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030956">
        <property type="term" value="C:glutamyl-tRNA(Gln) amidotransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070681">
        <property type="term" value="P:glutaminyl-tRNAGln biosynthesis via transamidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.60">
        <property type="entry name" value="Glu-tRNAGln amidotransferase C subunit, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00122">
        <property type="entry name" value="GatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036113">
        <property type="entry name" value="Asp/Glu-ADT_sf_sub_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003837">
        <property type="entry name" value="GatC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00135">
        <property type="entry name" value="gatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15004">
        <property type="entry name" value="GLUTAMYL-TRNA(GLN) AMIDOTRANSFERASE SUBUNIT C, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15004:SF0">
        <property type="entry name" value="GLUTAMYL-TRNA(GLN) AMIDOTRANSFERASE SUBUNIT C, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02686">
        <property type="entry name" value="GatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141000">
        <property type="entry name" value="Glu-tRNAGln amidotransferase C subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0934">Plastid</keyword>
    <keyword evidence="1" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9701899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="137" mass="15328" checksum="250D0AF853F56FA5" modified="2024-01-24" version="1">MGSRALLLLKATTAETLLFTSKSTFSKALIRNSTRSFSTRSALLPPDLPRLAETARISLTPHEVEEFAPKIRQVIDWFGQLQAVDLQSIEPSIRADTEGDNLRDDSPETFENREAIIAAIPSYEEPYLKVPKVLNKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A7U9</accession>
    <name>A0AA39A7U9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Expansin-like EG45 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_004282</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A7U9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22269">
        <property type="entry name" value="DPBB_EG45-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007112">
        <property type="entry name" value="Expansin/allergen_DPBB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009009">
        <property type="entry name" value="RlpA-like_DPBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47480">
        <property type="entry name" value="EG45-LIKE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47480:SF1">
        <property type="entry name" value="EG45-LIKE DOMAIN CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03330">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50842">
        <property type="entry name" value="EXPANSIN_EG45"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Expansin-like EG45" evidence="1">
        <location>
            <begin position="4"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like EG45" evidence="1">
        <location>
            <begin position="146"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9702466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="247" mass="25750" checksum="B5F2656F683992B4" modified="2024-01-24" version="1">MNAATACSGNDPSPFPSSNLFAAAGEGVWDNGAACGRQYRVRCISAPTPGTCKADQTIQVKIVDRAQTSVSRPSRDGATLVLSTTAFEAIANPSAAWVNVEFAQMSTSPHELHLRLLALLFILAGLVHICSGDVGTAGQYVPPYLPTACYGNDVSEFPSSNLFASAGEGIWDNGAACGRQYLVRCISAVVPMTCIAGQTIQIKIVDRAVTSVSRPSRDGATMVLSTTAFGAVANASASSINIEFQQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A9N5</accession>
    <name>A0AA39A9N5_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_005061</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A9N5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000485">
        <property type="entry name" value="Putative WD repeat-containing protein C17D11.16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000714">
        <property type="entry name" value="Transducin/WD40 repeat-like superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044285">
        <property type="entry name" value="PWP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14091">
        <property type="entry name" value="PERIODIC TRYPTOPHAN PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14091:SF0">
        <property type="entry name" value="PERIODIC TRYPTOPHAN PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="255"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="392"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9703566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="494" mass="54035" checksum="69ACA68129F7AEDA" modified="2024-01-24" version="1">MISAISWIPKGVSKPVPSVVEPPSKEEIEEMLKNGALERCGDSESEEDDETMDVDAAKQDDTVAHALAAADALGKTPKNSGTSFLDITDGLKELDMDHYDDEDDGIEVFSKGLGDTYYPSNDMDPYLKDNNDDDSEDLEDMTIKAEDSVIVCARNADDLSHLEVWIFEESEDGDSNMYVHHDVIIPAFPLCTAWLDCPLKGGEKGNFIAVGSMEPSIEIWDLDIIDEVQPFLVLGGIAEKKKKKKAKKTSVKYRKGSHTDSVLGLAWNKEYRNILASASADKLVKIWDVTSGECNITMDHHTDKVQAVAWNHHEPQVLLSGSFDRSVVMKDGRIPTHAGFKWSVTADVESLAWDPHTNHSFVVSLEDGTVQGFDVRTAKSDLASESKPAFTLHAHDQAVCTISYNALAPNLLATGSTDKMVKLWDLSNNQPSCVATRNPKAGAVFSISFSEDRPFLLAIGGSTGKLEVWDTLSEAAVSRKFGNHSSQGISKPEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AAK6</accession>
    <name>A0AA39AAK6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DDE Tnp4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_004601</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HARBI1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AAK6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058353">
        <property type="entry name" value="DUF8040"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045249">
        <property type="entry name" value="HARBI1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930:SF268">
        <property type="entry name" value="NUCLEASE HARBI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26138">
        <property type="entry name" value="DUF8040"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF8040" evidence="9">
        <location>
            <begin position="31"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="DDE Tnp4" evidence="8">
        <location>
            <begin position="157"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF26138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9702928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="231" mass="27021" checksum="717AD5161A78219C" modified="2024-01-24" version="1">MYAAYAAILVLVYHRSNYLERSISNEGDYEQHALMEKLTLMDNEDCYNQLRMGKDAFARLVNILWGTGRLRNSAHSNVEEQVAKFLHIIGHNLRNRTMKFYFKHSSETVSRHFHQVLRAIISLDDVFLKQFDGLKCPQEIKDNTKFWPYFKDCIGAIDGSHFRVKVSNDVVQRYRGRKYYPTQNILTTCSFDLKFTYVLPGWEGSASDSRIPDNALTRNFDKLIVPQGDYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AD24</accession>
    <name>A0AA39AD24_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_003062</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AD24"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17437">
        <property type="entry name" value="MFS_PLT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000025">
        <property type="entry name" value="probable polyol transporter 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050814">
        <property type="entry name" value="Myo-inositol_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044776">
        <property type="entry name" value="PLT1-6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020:SF49">
        <property type="entry name" value="SUGAR TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="6" id="KW-0769">Symport</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="33"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="160"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="190"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="330"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="357"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="388"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="455"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="12">
        <location>
            <begin position="37"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="502"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="503"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9704832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="526" mass="56757" checksum="310FB98FB9BE1782" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ADR9</accession>
    <name>A0AA39ADR9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NB-ARC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_003314</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ADR9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766:SF70">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RGA4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766">
        <property type="entry name" value="PLANT BROAD-SPECTRUM MILDEW RESISTANCE PROTEIN RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0611">Plant defense</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="3">
        <location>
            <begin position="90"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="4">
        <location>
            <begin position="235"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9705192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="505" mass="57211" checksum="9EDD60524F2929AD" modified="2024-01-24" version="1">MGTIQTYVLSPLPPKESWSLFLKHACAEMVEGESSNLMEFGHQVVEKCGGIPIQVRMSGNLMYSAKETEDWTSFEKLPSHLKPCFTFCSIFPKNSEIRSDDLVQLWIMHDLIKPISNQGDKEMEDIGEEYIKELLERSFFQDSEKREHGAYFSFRMHDLVHDLATSLAQQENIMLTFAAKNISRKIRHVSFSDEDWSGHEQQVLNFLGKLTDVQTILFPVDGVGLNSESIVNTCIERFKSMQVLDLSDSRFEALPESITSLKDLRFLSLKRNDRIMKLPDSISRLKNLHALMLGGCSELSNLPKDMELMINLRHLEITTKEEALPALNSFKSLRYLGVVGCANLKSLFGGRETFTALGTLFIHRCPSLVSLPCGIRHLSALKILRIDDCGTLDLLDGDDDNVPGLKSLRLLVIVKLPRLRTLPKWILQAAASAFNTLSCIVIESCPNFRGLPWEVLQKLDSYPKFKIENCPKSIVPGPDAYVGDKKMKSITDVLVRVKYSIFFHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ADV2</accession>
    <name>A0AA39ADV2_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_003675</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ADV2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9705707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="128" mass="14977" checksum="03A037CCA5EB3E3E" modified="2024-01-24" version="1">MLSSLKIKPLKTWIGLRSQSLLVKNRLIWVQYLLTLWDIMKARKNDESAVDDVEENPTVENDGLEHQKKQATSESPTETQLRKSTRERQPSRRYTSDEYLLLSNGGELTNYQEVLLYDKKKKMVKSYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AFS3</accession>
    <name>A0AA39AFS3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">STI1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_002006</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AFS3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051879">
        <property type="term" value="F:Hsp90 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000102">
        <property type="entry name" value="hsp70-Hsp90 organizing protein 3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.100:FF:000004">
        <property type="entry name" value="Putative stress-induced-phosphoprotein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000010">
        <property type="entry name" value="Stress-induced phosphoprotein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000020">
        <property type="entry name" value="Stress-induced phosphoprotein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.100:FF:000002">
        <property type="entry name" value="Stress-induced-phosphoprotein 1 (Hsp70/Hsp90-organizing)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041243">
        <property type="entry name" value="STI1/HOP_DP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006636">
        <property type="entry name" value="STI1_HS-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22904:SF533">
        <property type="entry name" value="HSP70-HSP90 ORGANIZING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22904">
        <property type="entry name" value="TPR REPEAT CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17830">
        <property type="entry name" value="STI1-HOP_DP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13414">
        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00727">
        <property type="entry name" value="STI1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0346">Stress response</keyword>
    <keyword evidence="6 11" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="11">
        <location>
            <begin position="2"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="11">
        <location>
            <begin position="36"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="11">
        <location>
            <begin position="70"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="STI1" evidence="13">
        <location>
            <begin position="146"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="11">
        <location>
            <begin position="245"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="11">
        <location>
            <begin position="384"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="11">
        <location>
            <begin position="418"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="11">
        <location>
            <begin position="452"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="domain" description="STI1" evidence="13">
        <location>
            <begin position="522"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="109"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="199"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="207"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="233"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9706818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="573" mass="64336" checksum="03500DBA95225A16" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AFW6</accession>
    <name>A0AA39AFW6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein ODORANT1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_001997</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AFW6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046394">
        <property type="term" value="P:carboxylic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000069">
        <property type="entry name" value="MYB transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000303">
        <property type="entry name" value="MYB transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015495">
        <property type="entry name" value="Myb_TF_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47994">
        <property type="entry name" value="F14D16.11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47994:SF5">
        <property type="entry name" value="F14D16.11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="9"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="9"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="62"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="62"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="131"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="138"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="163"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9706802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="268" mass="30264" checksum="89D6CEB1DBDED075" modified="2024-01-24" version="1">MGRQPCCDKLGVKKGPWTAEEDKKLINFILTNGQCCWRAVPKLAGLRRCGKSCRLRWTNYLRPDLKRGLLTESEEQLVIDLHARLGNRWSKIAARLPGRTDNEIKNHWNTHIKKKLLKMGIDPVTHEPFHKQAAAKETSPRADHLPENDTHNLQLPDPDGIINSDQDNSSSSPPENSSSTDESRLLDSICNDDSLMNCLWGDEMPLVDASWKFPSAGENFNNTGLPSWDDSCAWLLDCQDFGIHDFGIDCFNDIEVNSLNTLEMGDKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AHS3</accession>
    <name>A0AA39AHS3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nodulin-related protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_000070</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9707822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AHS3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010115">
        <property type="term" value="P:regulation of abscisic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040294">
        <property type="entry name" value="Nodulin-rel_1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35098">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35098:SF1">
        <property type="entry name" value="NODULIN-RELATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="99"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9707822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="140" mass="14997" checksum="C61F490DA4299774" modified="2024-01-24" version="1">MDPTSHHRPGSHPQKHHQPSSSELLSSAKVVAEAATATFHHESEKVDKSRVAGAAADILDAASHYGKLEEKSFGKYVEKAETYLHQYQSSHSATTTINSGHSTTNTTETHSSSHSGGGDSHSGGGYGDYLKMAEGFLKKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AK61</accession>
    <name>A0AA39AK61_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_000530</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AK61"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48434">
        <property type="entry name" value="(RAPE) HYPOTHETICAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48434:SF1">
        <property type="entry name" value="(RAPE) HYPOTHETICAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="197"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9708537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="249" mass="29837" checksum="B117DB51CEEA1F24" modified="2024-01-24" version="1">MNITDWNQKPFTSKTFSQPYHPMSYNYIDYMDVWYNMFYLQSYQHSWFIQFSRTCNFQFPAWFQKWWTFFGLLDSIFPPDIQEKFEFYKKRTSSQPFTQPKLLTFCSRLRIPWILTWNIIKKQEQPHPFPLSLSREFNIKWWDKFNHDFVCQQKVLELISARPGSSNSKDSSNSSQGPTQEEFLTLKSKCQASLAAATDLEQYRQELLQTLKQFDDQQENEDNEEIVISDDSYVDPEFILYGGPMGQAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D5L8</accession>
    <name>A0AA39D5L8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_025347</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D5L8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06609">
        <property type="entry name" value="STKc_MST3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000207">
        <property type="entry name" value="serine/threonine-protein kinase dst1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050629">
        <property type="entry name" value="STE20/SPS1-PAK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012:SF10">
        <property type="entry name" value="FI20177P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="15"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="273"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="407"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="511"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="653"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="41"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="275"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="310"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="339"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="367"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="407"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="418"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="433"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="466"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="514"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9671589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="717" mass="79441" checksum="D933B58DA75FA432" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D780</accession>
    <name>A0AA39D780_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">tRNA (guanine(37)-N1)-methyltransferase</fullName>
            <ecNumber evidence="10">2.1.1.228</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">M1G-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">tRNA [GM37] methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">tRNA methyltransferase 5 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_025475</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>guanosine(37) in tRNA + S-adenosyl-L-methionine = N(1)-methylguanosine(37) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36899"/>
            <dbReference type="Rhea" id="RHEA-COMP:10145"/>
            <dbReference type="Rhea" id="RHEA-COMP:10147"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="EC" id="2.1.1.228"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Mitochondrion matrix</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="10">Predominantly in the mitochondria and in the nucleus.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the TRM5 / TYW2 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM5/TYW2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.228" evidence="10"/>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D780"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052906">
        <property type="term" value="F:tRNA (guanine(37)-N1)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070901">
        <property type="term" value="P:mitochondrial tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002939">
        <property type="term" value="P:tRNA N1-guanine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.110:FF:000001">
        <property type="entry name" value="tRNA (guanine(37)-N1)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000225">
        <property type="entry name" value="tRNA (guanine(37)-N1)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.110">
        <property type="entry name" value="Met-10+ protein-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03152">
        <property type="entry name" value="TRM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030382">
        <property type="entry name" value="MeTrfase_TRM5/TYW2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056743">
        <property type="entry name" value="TRM5-TYW2-like_MTfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056744">
        <property type="entry name" value="TRM5/TYW2-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025792">
        <property type="entry name" value="tRNA_Gua_MeTrfase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245:SF43">
        <property type="entry name" value="TRNA (GUANINE(37)-N1)-METHYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245">
        <property type="entry name" value="TRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02475">
        <property type="entry name" value="TRM5-TYW2_MTfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25133">
        <property type="entry name" value="TYW2_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51684">
        <property type="entry name" value="SAM_MT_TRM5_TYW2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 10" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="SAM-dependent methyltransferase TRM5/TYW2-type" evidence="12">
        <location>
            <begin position="335"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="208"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="216"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="425"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="463"/>
            <end position="464"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="491"/>
            <end position="492"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="514"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9671782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="608" mass="69439" checksum="580300A51F4296BA" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D897</accession>
    <name>A0AA39D897_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_026222</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D897"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34835">
        <property type="entry name" value="OS07G0283600 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34835:SF34">
        <property type="entry name" value="OS08G0555500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9672877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="197" mass="22243" checksum="8E5C034FDA038051" modified="2024-01-24" version="1">MGFGGLLDLACWELRYELSAWIIFNYDFAYHQLNMAIGIVVPVTKEDVSKIMGILSNGEEIVQNLENLAIRDDFKKTFLIFACATLLAPNSKLEGMHDLWDTIWDGDASVQKNWSQIFLNYIEDGIREYRKNQPTYIRGCLIFLQLFYMTKFYLPSVTVNQPESVGNSHAPAVGGSSSACDDATEDVDVFIAKLTYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D8Z0</accession>
    <name>A0AA39D8Z0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_024151</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D8Z0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14707">
        <property type="entry name" value="bZIP_plant_BZIP46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000036">
        <property type="entry name" value="ABSCISIC ACID-INSENSITIVE 5-like protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043452">
        <property type="entry name" value="BZIP46-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22952">
        <property type="entry name" value="CAMP-RESPONSE ELEMENT BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22952:SF433">
        <property type="entry name" value="PROTEIN FD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BZIP" evidence="5">
        <location>
            <begin position="188"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="90"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="173"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9675084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="259" mass="28776" checksum="BFB5A94CE220D5AE" modified="2024-01-24" version="1">MFTMWSSLVDDDDDNRSSKRVSCSPSKSSTCSSHPPFSPSSPKKTMDEVWKDLSLSSLHHHSISTINNATANTHHAFRSTLLQDFLARPSNMDPLRTGTSTADPSSAGAATPFASPLPPPPTLLSLNSLPDFHCIDDTDPMKPHSHFHNNATAATPYMYPPFNVLAAASPAFPSFRKKRPRGSDDNSGDRRHKRLIKNRESAARSRARKQAYTNELELEVAHLIEENARLKRLQQKFCPEASAQLPKKHTLYRTSTAPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DA32</accession>
    <name>A0AA39DA32_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase</fullName>
            <ecNumber evidence="3">3.2.2.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_024648</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>NAD(+) + H2O = ADP-D-ribose + nicotinamide + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16301"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57967"/>
            <dbReference type="EC" id="3.2.2.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:16302"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the disease resistance TIR-NB-LRR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.6" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DA32"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061809">
        <property type="term" value="F:NAD+ nucleosidase activity, cyclic ADP-ribose generating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050832">
        <property type="term" value="P:defense response to fungus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043068">
        <property type="term" value="P:positive regulation of programmed cell death"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.430:FF:000002">
        <property type="entry name" value="Disease resistance protein (TIR-NBS-LRR class)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10140:FF:000007">
        <property type="entry name" value="Disease resistance protein (TIR-NBS-LRR class)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000386">
        <property type="entry name" value="Disease resistance protein RPS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045344">
        <property type="entry name" value="C-JID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017:SF570">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN (TIR-NBS CLASS)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20160">
        <property type="entry name" value="C-JID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0611">Plant defense</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="TIR" evidence="14">
        <location>
            <begin position="19"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1157"/>
            <end position="1198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1218"/>
            <end position="1270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1157"/>
            <end position="1167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1189"/>
            <end position="1198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1249"/>
            <end position="1258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1259"/>
            <end position="1270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00061488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9675805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1270" mass="143371" checksum="C49ABAF16F249D04" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DBD0</accession>
    <name>A0AA39DBD0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_024852</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9676057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DBD0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04051">
        <property type="entry name" value="C2_SRC2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044750">
        <property type="entry name" value="C2_SRC2/BAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32246:SF22">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32246">
        <property type="entry name" value="INGRESSION PROTEIN FIC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="1">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9676057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="192" mass="21066" checksum="6CDE8650C407F568" modified="2024-01-24" version="1">MKCKKFEITLISARNLEVRETQKPNVYAKFSIAGHPNTEKKTKVDKKGGANPAWDFTTACIIGKQAVEHDGVMLLIELYRSRTFGDQYIGEVRVSFKELFNRQRTLWGRLTTLFSCPKGDGVGKTVKYPVNNGGSDSSGVLKFSYRFRKEVIVDQPSGGGSALGLAASGAKWVGLIITVATGIDIPFSWEGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DD91</accession>
    <name>A0AA39DD91_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_021646</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DD91"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001023">
        <property type="entry name" value="Os07g0541700 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000178">
        <property type="entry name" value="serine/threonine-protein kinase PBS1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045874">
        <property type="entry name" value="LRK10/LRL21-25-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27009">
        <property type="entry name" value="RUST RESISTANCE KINASE LR10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="209"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="271"/>
            <end position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9679796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="569" mass="64779" checksum="E8FFDC22AE06F62F" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DDN8</accession>
    <name>A0AA39DDN8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NAC-A/B domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_021717</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May promote appropriate targeting of ribosome-nascent polypeptide complexes.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DDN8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005854">
        <property type="term" value="C:nascent polypeptide-associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22054">
        <property type="entry name" value="NAC_NACA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14358">
        <property type="entry name" value="UBA_NAC_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.30:FF:000002">
        <property type="entry name" value="Nascent polypeptide-associated complex (NAC), alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000006">
        <property type="entry name" value="Putative nascent polypeptide-associated complex subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.30">
        <property type="entry name" value="Nascent polypeptide-associated complex domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016641">
        <property type="entry name" value="EGD2/NACA0like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044034">
        <property type="entry name" value="NAC-like_UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038187">
        <property type="entry name" value="NAC_A/B_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002715">
        <property type="entry name" value="Nas_poly-pep-assoc_cplx_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21713">
        <property type="entry name" value="NASCENT POLYPEPTIDE ASSOCIATED COMPLEX ALPHA SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01849">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19026">
        <property type="entry name" value="UBA_HYPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015901">
        <property type="entry name" value="NAC_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01407">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51151">
        <property type="entry name" value="NAC_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAC-A/B" evidence="3">
        <location>
            <begin position="62"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="24"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9679900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="209" mass="22940" checksum="BAF925C7A62A0C53" modified="2024-01-24" version="1">MPEAEAEQVASLEEDLQKKKLLEEEPVVEDVNEEEDDDDDEDDEDDEKEDGAQGANEGSKQSRSEKKSRKAMLKLGMKPVTGVGRVTIKRTKNILFFISKPDVFKSPNSETYVIFGEAKIEDLSSQLQTQAAQQFRMPDMGSVMAKSDISAAAAGTQADEEEEVIDETGVEPRDIDLVMTQAGVSRSKAVKALKTHNGDIVSAIMELTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DE16</accession>
    <name>A0AA39DE16_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_019568</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DE16"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9680290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="297" mass="32160" checksum="484D3A8ADDD6B232" modified="2024-01-24" version="1">MSVSYTLVLNWTRSVPQYSAEFDSGSVSYSPVHNQTRSLAWYSAEFDSRYVSYFLVLNWTQSLARYSAEFDSRSVSYSSILNRTRSLPGYSAKFHSGSVSLSRILGSSTGHDIFPGTQPNSIPGLSRILGSSTGHDLFFGTYSNSIPGLSLILGSSTGHDIFPGTQPNSIPGLSRILRSSTGHDLFSGTQPNSIPRLSLFSGAQSNTISSPVLSRIRFPVCLVFSNSQSNTISSRVLSRIRFPVCLVYLQSSTGHDLFPGAQPNSIPSLSRILGSSTGHDIFPGTQLNSIPSLSRIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DFD7</accession>
    <name>A0AA39DFD7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Amidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_019455</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DFD7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000120">
        <property type="entry name" value="Amidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895:SF73">
        <property type="entry name" value="AMIDASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895">
        <property type="entry name" value="TRANSAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="1">
        <location>
            <begin position="172"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9680167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="625" mass="68660" checksum="B64EFDD294C3C93C" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DFN8</accession>
    <name>A0AA39DFN8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">superoxide dismutase</fullName>
            <ecNumber evidence="4">1.15.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_018202</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>2 superoxide + 2 H(+) = H2O2 + O2</text>
            <dbReference type="Rhea" id="RHEA:20696"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:18421"/>
            <dbReference type="EC" id="1.15.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Cu-Zn superoxide dismutase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.15.1.1" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DFN8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071484">
        <property type="term" value="P:cellular response to light intensity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071457">
        <property type="term" value="P:cellular response to ozone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071472">
        <property type="term" value="P:cellular response to salt stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071493">
        <property type="term" value="P:cellular response to UV-B"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00305">
        <property type="entry name" value="Cu-Zn_Superoxide_Dismutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.200:FF:000001">
        <property type="entry name" value="Superoxide dismutase [Cu-Zn]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.200">
        <property type="entry name" value="Superoxide dismutase, copper/zinc binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036423">
        <property type="entry name" value="SOD-like_Cu/Zn_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024134">
        <property type="entry name" value="SOD_Cu/Zn_/chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018152">
        <property type="entry name" value="SOD_Cu/Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001424">
        <property type="entry name" value="SOD_Cu_Zn_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10003">
        <property type="entry name" value="SUPEROXIDE DISMUTASE CU-ZN -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00080">
        <property type="entry name" value="Sod_Cu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00068">
        <property type="entry name" value="CUZNDISMTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49329">
        <property type="entry name" value="Cu,Zn superoxide dismutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00087">
        <property type="entry name" value="SOD_CU_ZN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="9" id="KW-0186">Copper</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Superoxide dismutase copper/zinc binding" evidence="11">
        <location>
            <begin position="16"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9682209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="151" mass="15363" checksum="41656DA26C3DB6DE" modified="2024-01-24" version="1">MVKAVAVPNSNEGACGTIYFAEEGDGSTTVTGSLSGLKPGLHGFHVHALGDTTNGCMSTGPHFNPDGKEHGAPEDENRHAGDLGNVIVGEDGTVNFKIVDKQIPLTGSNSIVGRAVVVHADPDDLGKGGHELSKSTGNAGGRVACGMRCET</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DGH8</accession>
    <name>A0AA39DGH8_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_020281</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9681322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DGH8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9681322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="147" mass="16539" checksum="FD12F99519873A6F" modified="2024-01-24" version="1">MKMQYLFPSISETPSRINPPARFIPRIIEYILPCSFHSLPHLASSSSLMQRFSPSRKNPMLSEPNRTHDDTPPFFSTMNPWIFPFLSSTTISNTLDSTVKLHSFLLFRDAELSSPSSPSHSPSHSLSPSLALSLIAKFSIVYPFIVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DGX0</accession>
    <name>A0AA39DGX0_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_017910</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9681757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DGX0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35702">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35702:SF1">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041336809" evidence="2">
        <location>
            <begin position="25"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="201"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9681757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="232" mass="25216" checksum="F3E32D0400AD03CD" modified="2024-01-24" version="1">MNLPRKRVQLLIFVVGIVALSITAEKCRQLLGEDGSSQSGKFTLLNCFDMSSGTLACTVKEGVKLYFYNIRAAHVERARHHAIEGAISDALTQGLNAKDAAKQAQKEGAKAAKLATRQAKRIIGPIISSGWDFFEAIYYGGTWTEGFLRGTGTLFGTYTGGFLGEQRLGRVGYLVGSHLGSWVGGRIGLMVYDVGQWKESPMDMSSEVPEDPGSYESPTYESSEAQEDSGIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DH84</accession>
    <name>A0AA39DH84_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Expansin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_016492</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Causes loosening and extension of plant cell walls by disrupting non-covalent bonding between cellulose microfibrils and matrix glucans. No enzymatic activity has been found.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Secreted</location>
            <location evidence="7">Cell wall</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Membrane</location>
            <topology evidence="7">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the expansin family. Expansin A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DH84"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009664">
        <property type="term" value="P:plant-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22274">
        <property type="entry name" value="DPBB_EXPA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.10:FF:000001">
        <property type="entry name" value="Expansin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.760:FF:000001">
        <property type="entry name" value="Expansin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.760">
        <property type="entry name" value="Expansin, cellulose-binding-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007118">
        <property type="entry name" value="Expan_Lol_pI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002963">
        <property type="entry name" value="Expansin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007112">
        <property type="entry name" value="Expansin/allergen_DPBB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007117">
        <property type="entry name" value="Expansin_CBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036749">
        <property type="entry name" value="Expansin_CBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009009">
        <property type="entry name" value="RlpA-like_DPBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31867">
        <property type="entry name" value="EXPANSIN-A15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03330">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01357">
        <property type="entry name" value="Expansin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01226">
        <property type="entry name" value="EXPANSIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01225">
        <property type="entry name" value="EXPANSNFAMLY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00837">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49590">
        <property type="entry name" value="PHL pollen allergen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50843">
        <property type="entry name" value="EXPANSIN_CBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50842">
        <property type="entry name" value="EXPANSIN_EG45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0134">Cell wall</keyword>
    <keyword evidence="6 7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041480655" description="Expansin" evidence="7">
        <location>
            <begin position="26"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like EG45" evidence="8">
        <location>
            <begin position="48"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like CBD" evidence="9">
        <location>
            <begin position="170"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9684026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="252" mass="26635" checksum="1AE35FF5869D4AB4" modified="2024-01-24" version="1">MATAAFSSISLALFLFCLCLQGTNGDYGGWEGGHATFYGGGDASGTMGGACGYGNLYSQGYGTNTAALSTALFNSGLSCGACYEMKCNDDPKWCIPGTLTVTATNFCPPNLALSNTNGGWCNPPLQHFDLAEPAFLQIAQYRAGIVPVSFRRVPCAKKGGIRFTVNGHSYFNLVLITNVAGAGDVRAVSIKGSNTGWQPMSRNWGQNWQSNSYLNGQTLSFQVTASDGRTVTSLNVAPAGWQFGQTYEGAQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DJ87</accession>
    <name>A0AA39DJ87_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">15-cis-phytoene synthase</fullName>
            <ecNumber evidence="4">2.5.1.32</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_015061</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 (2E,6E,10E)-geranylgeranyl diphosphate = 15-cis-phytoene + 2 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:34475"/>
            <dbReference type="ChEBI" id="CHEBI:27787"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58756"/>
            <dbReference type="EC" id="2.5.1.32"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carotenoid biosynthesis; phytoene biosynthesis; all-trans-phytoene from geranylgeranyl diphosphate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the phytoene/squalene synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.32" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9685989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DJ87"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009536">
        <property type="term" value="C:plastid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046905">
        <property type="term" value="F:15-cis-phytoene synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004311">
        <property type="term" value="F:geranylgeranyl diphosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051996">
        <property type="term" value="F:squalene synthase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016117">
        <property type="term" value="P:carotenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00683">
        <property type="entry name" value="Trans_IPPS_HH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.600.10:FF:000004">
        <property type="entry name" value="Phytoene synthase chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002060">
        <property type="entry name" value="Squ/phyt_synthse"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019845">
        <property type="entry name" value="Squalene/phytoene_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044843">
        <property type="entry name" value="Trans_IPPS_bact-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033904">
        <property type="entry name" value="Trans_IPPS_HH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31480">
        <property type="entry name" value="BIFUNCTIONAL LYCOPENE CYCLASE/PHYTOENE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00494">
        <property type="entry name" value="SQS_PSY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01212">
        <property type="entry name" value="Phytoene_synthase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01018">
        <property type="entry name" value="Squalene/Phytoene_Synthase_Lik"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01044">
        <property type="entry name" value="SQUALEN_PHYTOEN_SYN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01045">
        <property type="entry name" value="SQUALEN_PHYTOEN_SYN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0125">Carotenoid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9685989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="386" mass="43997" checksum="DDFA6E9311D56181" modified="2024-01-24" version="1">MSGVLLWVVCPKENASFSSLFSFIPRNGMQRRFKLGSRLKFSTGFSAVANAVANPSRSSEERVYEVVLRQAALVRDLFKPDGMVNWDLLNEAYDRCGEVCAEYAKTFYLGTLLMTPERRRAVWAIYVWCRRTDELVDGPNASHMSPKALDRWEQRLIDLFEGHPYDMYDAALSDTVAKYPVDVQPFKDMIEGMRLDLRKSRYNSFDELYLYCYYVAGTVGLMSVPVMGISPDSKASTESVYNAALALGIANQLTNILRDVGEDARRGRIYLPQDELERAGLSDDDILRGKVTDKWRSFMKGQIKRARMFFNEAEKGVRELNSASRWPVWASLMLYRQILDAIEANDYDNFTKRAYVGKLKKLVSLPIAYGSAMMGPSSKLTQLVRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DJM1</accession>
    <name>A0AA39DJM1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">VQ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_015038</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9685960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DJM1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008889">
        <property type="entry name" value="VQ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05678">
        <property type="entry name" value="VQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VQ" evidence="2">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9685960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="186" mass="20243" checksum="341D5D0B029F13B7" modified="2024-01-24" version="1">MGKKVMSEASLKIPHKNDKNKLNSLIKVLRPKVYITDSSSFKKLVQELTGNGTSVAPPPPPPPPPRPPPSTVQRAAEKFPVIGVDEDFVVGPGNSVENSGYSSSEVCNLQPSFMVESRQVGACDEAILDMTALDDFRMNQDADVLGFQDMESWVLQIDPFSLCGNGYAQSFEEAACIYDYDLAGLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DKG4</accession>
    <name>A0AA39DKG4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_016214</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DKG4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06606">
        <property type="entry name" value="STKc_MAPKKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000466">
        <property type="entry name" value="MAP kinase kinase kinase18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052751">
        <property type="entry name" value="Plant_MAPKKK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48011">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTIONAL COMPLEX SUBUNIT CAF120-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48011:SF5">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="15"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9687641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="363" mass="40137" checksum="8C9698DE1B2E168C" modified="2024-01-24" version="1">MSLDKQCLAPQPGEWVKGKMVGSGSFGTVHLAMSKATGGLFVVKSAQEGPGLKSLENEANMLEKLHSPYIVRCMGRDSFVGRQGEGRLNLFMEYMAGGSLSNVADKFGGILEEKVIRLYTREILQGLKYLHKNEIVHCDLKCQNVLLGSSGNVKLADFGCAKRLRDLKSMGASMTSWQSISGTPLWMAPEVLRNEGVTLASDIWSLGCTIIEMATGRPPWSGEVSNPMAAVMMIACSNKIPQIPTHLSKEGLDFLAKCLDRNPAKRWTAEELLSHPFVSRHLERENLRKEDFDSPKSILDVRNHEDGSDSDQPECVDGCGFFRRISFSKRHGCKRKKMNKKSRKQLVEGDLASSGNWVTVRSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DKH4</accession>
    <name>A0AA39DKH4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">WRKY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_016247</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.80:FF:000004">
        <property type="entry name" value="WRKY transcription factor 65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.80">
        <property type="entry name" value="WRKY domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057135">
        <property type="entry name" value="At4g27190-like_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003657">
        <property type="entry name" value="WRKY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036576">
        <property type="entry name" value="WRKY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044810">
        <property type="entry name" value="WRKY_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31282">
        <property type="entry name" value="WRKY TRANSCRIPTION FACTOR 21-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23247">
        <property type="entry name" value="LRR_RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03106">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00774">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118290">
        <property type="entry name" value="WRKY DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50811">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="WRKY" evidence="9">
        <location>
            <begin position="1221"/>
            <end position="1287"/>
        </location>
    </feature>
    <feature type="domain" description="WRKY" evidence="9">
        <location>
            <begin position="1302"/>
            <end position="1368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="34"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="44"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9687686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1386" mass="158889" checksum="90B0DFF0D5FE19A1" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DL96</accession>
    <name>A0AA39DL96_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Lycopene epsilon-cyclase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_014037</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Carotenoid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the lycopene cyclase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DL96"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009536">
        <property type="term" value="C:plastid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009975">
        <property type="term" value="F:cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016860">
        <property type="term" value="F:intramolecular oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016117">
        <property type="term" value="P:carotenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000101">
        <property type="entry name" value="lycopene epsilon cyclase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010108">
        <property type="entry name" value="Lycopene_cyclase_b/e"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01790">
        <property type="entry name" value="carotene-cycl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39757">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39757:SF3">
        <property type="entry name" value="LYCOPENE EPSILON CYCLASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05834">
        <property type="entry name" value="Lycopene_cycl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0125">Carotenoid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0934">Plastid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="475"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9688089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="529" mass="59241" checksum="3462349432613946" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DPE1</accession>
    <name>A0AA39DPE1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_013159</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DPE1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.330:FF:000001">
        <property type="entry name" value="Protein SRG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050295">
        <property type="entry name" value="Plant_2OG-oxidoreductases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47991">
        <property type="entry name" value="OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="6">
        <location>
            <begin position="208"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9690865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="365" mass="40913" checksum="BB0B9F973B88E1C8" modified="2024-01-24" version="1">MDAQKLTSLGGSLPVPCVQELVKTTLTTVPPRYLRPEQDPPFLSDSSTTCSSIHVPVIDFNCLVSGDSMDSELDKLHYACKEWGFFQLTNHGVISTLVEKVKVEVQEFFNLPMEEKKKFWQQPEDIQGFGQAFVLSEEQKLDWADKFYMITLPTHLRKPHLFSKLPVSLRETLEVYAVELRNLAMTILGFLAKALKMEASDMKELFEEGHQAMRNYYPPCPQPDQVIGLTPHSDSVGLTILLQVNEMDGLQIRKDGMWIPIKPLPGAFIVNIGDILEIVTNGAYRSIEHRATVNSIKERLSIATIYSPKLNGDMGPAPSLVTPDSPALFKRIGVADYFKGLLSRELRGKSYLEVLQTGTAEAQNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DTQ9</accession>
    <name>A0AA39DTQ9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Splicing factor Cactin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_010642</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CACTIN family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DTQ9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019134">
        <property type="entry name" value="Cactin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018816">
        <property type="entry name" value="Cactin_central"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21737">
        <property type="entry name" value="POLYGLUTAMINE BINDING PROTEIN 1/MARVEL MEMBRANE-ASSOCIATING DOMAIN CONTAINING 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21737:SF4">
        <property type="entry name" value="SPLICING FACTOR CACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10312">
        <property type="entry name" value="Cactin_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09732">
        <property type="entry name" value="CactinC_cactus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01050">
        <property type="entry name" value="CactinC_cactus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Splicing factor cactin central" evidence="6">
        <location>
            <begin position="183"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="Splicing factor Cactin C-terminal" evidence="5">
        <location>
            <begin position="530"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="96"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="393"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="155"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="37"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="52"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="100"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00034534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF09732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF10312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9695260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="654" mass="76131" checksum="57B996E8E0D5F3EB" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DUK8</accession>
    <name>A0AA39DUK8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">STAS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_008394</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DUK8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008271">
        <property type="term" value="F:secondary active sulfate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07042">
        <property type="entry name" value="STAS_SulP_like_sulfate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.750.24:FF:000002">
        <property type="entry name" value="Sulfate transporter 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="entry name" value="STAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018045">
        <property type="entry name" value="S04_transporter_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011547">
        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001902">
        <property type="entry name" value="SLC26A/SulP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00815">
        <property type="entry name" value="sulP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11814">
        <property type="entry name" value="SULFATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01740">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00916">
        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SpoIIaa-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01130">
        <property type="entry name" value="SLC26A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="72"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="127"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="206"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="259"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="299"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="330"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="358"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="390"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="domain" description="STAS" evidence="7">
        <location>
            <begin position="448"/>
            <end position="571"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9696130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="590" mass="64338" checksum="7BCE88FD57DB3DBD" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DX82</accession>
    <name>A0AA39DX82_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transcription factor CBF/NF-Y/archaeal histone domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_009212</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NFYB/HAP3 subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DX82"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016602">
        <property type="term" value="C:CCAAT-binding factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22907">
        <property type="entry name" value="HFD_NFYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000035">
        <property type="entry name" value="Nuclear transcription factor Y subunit B-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027113">
        <property type="entry name" value="Transc_fact_NFYB/HAP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003956">
        <property type="entry name" value="Transcrpt_fac_NFYB/HAP3_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11064">
        <property type="entry name" value="CCAAT-BINDING TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11064:SF122">
        <property type="entry name" value="NUCLEAR TRANSCRIPTION FACTOR Y SUBUNIT B-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00615">
        <property type="entry name" value="CCAATSUBUNTA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00685">
        <property type="entry name" value="NFYB_HAP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription factor CBF/NF-Y/archaeal histone" evidence="7">
        <location>
            <begin position="39"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9697312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="200" mass="22480" checksum="01CDE2C8E30A3679" modified="2024-01-24" version="1">MEDDESHANAATTTNPESPCLKSSSGSGSNSINKEQDRFLPIANVGRIMKKVIPGNGKISKDAKETVQECVSEFISFVTGEASDKCQREKRKTINGEDIIWAITTLGFEDYVSPLKQYLSKYREIEGEKLNIPKQHRSEQRLQQQEQEQNLPYASVYSSTTLISQPPFVAADQPFPLPFSADSIQKQLHQQEHFDSGGRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DXI0</accession>
    <name>A0AA39DXI0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FAR1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_009419</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DXI0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004330">
        <property type="entry name" value="FAR1_DNA_bnd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46328">
        <property type="entry name" value="FAR-RED IMPAIRED RESPONSIVE (FAR1) FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46328:SF15">
        <property type="entry name" value="PROTEIN FAR1-RELATED SEQUENCE 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03101">
        <property type="entry name" value="FAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAR1" evidence="1">
        <location>
            <begin position="45"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9697582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="211" mass="24263" checksum="AB752ABA063B76FD" modified="2024-01-24" version="1">MTVDSDMGAGNVEPSAETGSVAFEEDSVVEPCVGMEFESEDAAKEFYDEYARRVGFIMRIDQCRRSEVDKRILSRRLSCNKQGFYVKIRDQFGPVRKPRPSTRQGCKAMMLVKLNKSGKWAVTRFVKDHTHPLVVSSRPSRSSMDSKDRRIQELTMELEHQDQLCELYRGQLITFLKNVEEQTEVLSTKIEVAVNSVRKVESEGQKHSRHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DXY0</accession>
    <name>A0AA39DXY0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Armadillo repeat-containing protein 8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_007215</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DXY0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034657">
        <property type="term" value="C:GID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000416">
        <property type="entry name" value="Armadillo repeat-containing protein 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000440">
        <property type="entry name" value="Armadillo repeat-containing protein 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038739">
        <property type="entry name" value="ARMC8/Vid28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15651">
        <property type="entry name" value="ARMADILLO REPEAT-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15651:SF7">
        <property type="entry name" value="ARMADILLO REPEAT-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9698002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="655" mass="70553" checksum="389B999306FF1072" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DZW1</accession>
    <name>A0AA39DZW1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cysteine-rich receptor-like protein kinase 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_004625</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DZW1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23509">
        <property type="entry name" value="Gnk2-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000336">
        <property type="entry name" value="Cysteine-rich receptor-like protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.430.20:FF:000014">
        <property type="entry name" value="Cysteine-rich receptor-like protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.430.20:FF:000017">
        <property type="entry name" value="Cysteine-rich receptor-like protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.430.20">
        <property type="entry name" value="Gnk2 domain, C-X8-C-X2-C motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052059">
        <property type="entry name" value="CR_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002902">
        <property type="entry name" value="GNK2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038408">
        <property type="entry name" value="GNK2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47973">
        <property type="entry name" value="CYSTEINE-RICH RECEPTOR-LIKE PROTEIN KINASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01657">
        <property type="entry name" value="Stress-antifung"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51473">
        <property type="entry name" value="GNK2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="266"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="13">
        <location>
            <begin position="29"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="13">
        <location>
            <begin position="137"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="326"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="629"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="650"/>
            <end position="671"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9702953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="671" mass="74062" checksum="12449A93473A1093" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E0V3</accession>
    <name>A0AA39E0V3_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_006387</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E0V3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9701017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="64" mass="7523" checksum="0B308468E7ACFF62" modified="2024-01-24" version="1">MIAKMFWEISDVYDIKSYCRLLSLKMLRWQIIQQLFMANRAPASPMEIDDGNAKRDGKDETDHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E1G1</accession>
    <name>A0AA39E1G1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Cation/H(+) antiporter 18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_001579</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the monovalent cation:proton antiporter 2 (CPA2) transporter (TC 2.A.37) family. CHX (TC 2.A.37.4) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E1G1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006885">
        <property type="term" value="P:regulation of pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1530.20:FF:000003">
        <property type="entry name" value="Cation/H(+) antiporter 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057291">
        <property type="entry name" value="CHX17_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057290">
        <property type="entry name" value="CHX17_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050794">
        <property type="entry name" value="CPA2_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32468">
        <property type="entry name" value="CATION/H + ANTIPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32468:SF34">
        <property type="entry name" value="CATION_H(+) ANTIPORTER 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23256">
        <property type="entry name" value="CHX17_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23259">
        <property type="entry name" value="CHX17_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0050">Antiport</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0630">Potassium</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="62"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="98"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="127"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="161"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="195"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="228"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="261"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="326"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="358"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="390"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="12">
        <location>
            <begin position="50"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H(+) antiporter central" evidence="13">
        <location>
            <begin position="468"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H(+) antiporter C-terminal" evidence="14">
        <location>
            <begin position="611"/>
            <end position="758"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF23256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF23259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9706076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="778" mass="84240" checksum="3C060425C5BBA006" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E1V0</accession>
    <name>A0AA39E1V0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_005052</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AAA ATPase family. BCS1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E1V0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19510">
        <property type="entry name" value="RecA-like_BCS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025753">
        <property type="entry name" value="AAA_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058017">
        <property type="entry name" value="At3g28540-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050747">
        <property type="entry name" value="Mitochondrial_chaperone_BCS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23070">
        <property type="entry name" value="BCS1 AAA-TYPE ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14363">
        <property type="entry name" value="AAA_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25568">
        <property type="entry name" value="AAA_lid_At3g28540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="8">
        <location>
            <begin position="246"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="452"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="452"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9703555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="476" mass="54045" checksum="0D2690DF9588A40F" modified="2024-01-24" version="1">METMGDFFSGMPAVATRLFSFYASLQAFIVLIRTMINELIPDQTRTYVLSKLQTYWFAPPFSQLTLLIEEDHGMTPNEIYYAIQAYLDTKIPPSAERLKVGKTPGDNNLNVTIAKGQEVPDSFQNIRLKWVLGTKRDYNGFDSTFELSFDKKYKEIVLQSYLPHIIARANDLKVTDKVLKLYSRSHTQRGGDDSYDYTGDWGFTTLKHPATFDTMAMDPELKKAMIDDLNRFVARKEYYKRVGKPWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYHLELNSIRSDNELKQILVSTTSKSMIVIEDIDCNAETRDRGDSLYQYEPTIAKLTLSGILNFTDGLWSSCGEQRIIVFTTNHKDLLDPALLRPGRMDMQIYMSYCTYDGFKTLASNYLGVTDHPLFGEIETLLKNTEVTPAEIGEEFMRSDDADVALGGLVEFINRKKIGGDRMEMERATKVDEGRENDDESEGIGVAST</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E212</accession>
    <name>A0AA39E212_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serine carboxypeptidase-like 18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_003376</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E212"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019748">
        <property type="term" value="P:secondary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000072">
        <property type="entry name" value="Serine carboxypeptidase-like 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF224">
        <property type="entry name" value="SERINE CARBOXYPEPTIDASE-LIKE 7 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9705293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="420" mass="48145" checksum="1A32901C0CC533DE" modified="2024-01-24" version="1">MDDVQLFYYFIESERNPRDDPLLLWLTGGPGCSSFSGLVYEVGPFSFDYAKSSENLPTFKLNPYSWTKVANMIFLDAPVGTGFSYSRTAEGYNMNDTLSASQIYTFLRKWLINHPKFKKNPLYISGDSYSGIIIPMVVQEISNGNDAEKEPKMNIQGYTIGNPVTDHFSDFNSRIAYTHRVGILSDELYEAIKASCDGKYVDVDPSNADCTNNLKVYTQCIAKLDPEHILEPNCAYLSPKPNASKWDQISLKGINSINLLLQSPPQKPEPWCRRYNYLFSYLWANDKTVQKALYVREGTIKDWVRCNESLSYTSNVFSSVDYHRNLTKKAYRALIYSGDHDMLIPYVGTQQWIASLNLNISDDWQPWFVDGQVAGFRVEYSHDKYLMTFATIKGGGHTAPGYRPKESFAMAYRWFFHYFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E2Y0</accession>
    <name>A0AA39E2Y0_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_002567</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the amino acid-polyamine-organocation (APC) superfamily. Polyamine:cation symporter (PHS) (TC 2.A.3.12) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E2Y0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015203">
        <property type="term" value="F:polyamine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000041">
        <property type="entry name" value="Amino acid permease, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044566">
        <property type="entry name" value="RMV1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45826:SF2">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45826">
        <property type="entry name" value="POLYAMINE TRANSPORTER PUT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0769">Symport</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="57"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="171"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="195"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="268"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="354"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="384"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="415"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="443"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9707591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="491" mass="54016" checksum="AE0B0A06618264A5" modified="2024-01-24" version="1">MQSPPSAPQNDVVGVPTPTVETSREEAPAMGECKGAQYVGVDVDEVAPLGVDNFKKVSVLPLVFLIFYEVSGGAFGIEDAVRAADPLLALVGFLIFPFIWSIPEALITAEMGTMFPENGGYVVWVSSALGPYWGFQLGWMKWFSGVIDNALYPLLFLDYLSPTVPALSGGLPRIAAVLALIVVLTYMNYRGLTIVGWAAVLLGLFSILPFVIMGLVSIPMLKPSRWSVIEKDVNWSLYLTTLFWNLNDWDSISTIAGEVENPKKTLPMALFCALILVVRSYFFPLLIGTGALSLDREAWTDGYVSHVAKMIGGVWLSWWNLGMFVAEMSSDSFQLLGMAERGMLPSVFAKRSRYGTPLIGILLSASGVLLLSWMSFQETIPAENFLYCFGMILEFIAFVRLRIKYPAAPRPYKIPLGTIGSILMCIPPTILICIMVALSSLKVVVVSLIVVIIGLLLQPCLKCAERKKWLKFSVSSDLPDLMVKIMRTSYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E4D1</accession>
    <name>A0AA39E4D1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_000854</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E4D1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025322">
        <property type="entry name" value="PADRE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33413:SF4">
        <property type="entry name" value="D-RIBOSE-BINDING PERIPLASMIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33413">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14009">
        <property type="entry name" value="PADRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="149"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9709076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="175" mass="19514" checksum="37BE258CE4CC4B06" modified="2024-01-24" version="1">MGNCQAIDAAALVIQHPCGRIERLYWTVSAGEVMRMNPGHYVSLIIPLPISQDHDTPDNKTVRFTRVKLLRPTDTLVLGHAYRLITSQEVMKVLRAKKYAKMKKNQVESAEKLQGMAERQSSSCEEDGGRSEMEKSNQVVKHERHRPRAASTTSAAAARSKSWRPSLQSISEAGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E5F4</accession>
    <name>A0AA39E5F4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cytochrome P450 78A5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_000025</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9707754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E5F4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048608">
        <property type="term" value="P:reproductive structure development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11076">
        <property type="entry name" value="CYP78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000016">
        <property type="entry name" value="Cytochrome P450 78A5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051996">
        <property type="entry name" value="Cytochrome_P450_78A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47946">
        <property type="entry name" value="CYTOCHROME P450 78A7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47946:SF14">
        <property type="entry name" value="CYTOCHROME P450 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8 9" id="KW-0349">Heme</keyword>
    <keyword evidence="6 8 9" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="23"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="8">
        <location>
            <position position="462"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9707754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="517" mass="57468" checksum="5D5E114601BD3CB7" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YH57</accession>
    <name>A0AA38YH57_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WDR5-like beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_026728</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YH57"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000228">
        <property type="entry name" value="COMPASS-like H3K4 histone methylase component WDR5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059122">
        <property type="entry name" value="Beta-prop_WDR5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847:SF637">
        <property type="entry name" value="WD REPEAT DOMAIN 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25175">
        <property type="entry name" value="Beta-prop_WDR5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002394">
        <property type="entry name" value="GN-bd_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="WDR5-like beta-propeller" evidence="4">
        <location>
            <begin position="18"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="19"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="62"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="104"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="146"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="189"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="249"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="276"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9670364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="314" mass="34471" checksum="EF9B4B7921221644" modified="2024-01-24" version="1">MASDPSSEPINPYKLKQTLAAHKRAVSAVKFSSDGLLLGSSSADKTLRTWSTSGDFSTLQEFHGHDQGISDLAFSSDSRHVCSASDDKTVRLWDVETGSLIKTLQGHTNYVFCVNFNPQSNMIVSGSFDETVRVWDVKTGKCLKVLPAHSDPVTAANFNRDGSLIVSSSYDGLCRIWDASTGHCMKTLIDDENPPVSFVKFSPNGKFILVGTLDNTLRLWNFSTGKFLKTYTGHVNSKYCISSTFSVTNGKYIVGGSEDNCVYLWELQTRKVVQKLEGHTDTVISVSCHPSQNMIASGALGSDKTVKIWTQERD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YHD6</accession>
    <name>A0AA38YHD6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Mitochondrial outer membrane protein porin 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_026800</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic mitochondrial porin (TC 1.B.8.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YHD6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046930">
        <property type="term" value="C:pore complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015288">
        <property type="term" value="F:porin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008308">
        <property type="term" value="F:voltage-gated monoatomic anion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07306">
        <property type="entry name" value="Porin3_VDAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.10:FF:000003">
        <property type="entry name" value="Outer mitochondrial membrane protein porin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.10">
        <property type="entry name" value="Porin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023614">
        <property type="entry name" value="Porin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001925">
        <property type="entry name" value="Porin_Euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027246">
        <property type="entry name" value="Porin_Euk/Tom40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11743:SF23">
        <property type="entry name" value="MITOCHONDRIAL OUTER MEMBRANE PROTEIN PORIN 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11743">
        <property type="entry name" value="VOLTAGE-DEPENDENT ANION-SELECTIVE CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01459">
        <property type="entry name" value="Porin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56935">
        <property type="entry name" value="Porins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00558">
        <property type="entry name" value="EUKARYOTIC_PORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0626">Porin</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9670484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="276" mass="29322" checksum="BA6D57F7B220E13C" modified="2024-01-24" version="1">MSKGPGLFADIGKKAKDLLTRDYISDQKFTVSTYSDTGVAITSTAVKKGGLSTGDVAVLYKYKNSVIDVKADTGSNINTTLTFTEIIPSTKTIASFKFPDYNSGKLEVQYFHDHATLTTSVALNQSPAVDLSVTIGTPSIAFGAEAGYDTTSGCFTKYTAGISVAKPDSYASIILGDKGDTIRASYVHHLDSLKKSAAVGEISRRFSTNDNTFTVGGSYAIDPLTTVKAKLNNHGNLGALLQHEIIPKSILTLSGEFDTKALDKTPRFGLALALKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YHN6</accession>
    <name>A0AA38YHN6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]</fullName>
            <ecNumber evidence="9">1.3.1.88</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_026912</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>5,6-dihydrouridine(16) in tRNA + NAD(+) = uridine(16) in tRNA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53380"/>
            <dbReference type="Rhea" id="RHEA-COMP:13543"/>
            <dbReference type="Rhea" id="RHEA-COMP:13544"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.88"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="12">
            <dbReference type="Rhea" id="RHEA:53382"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>5,6-dihydrouridine(16) in tRNA + NADP(+) = uridine(16) in tRNA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53376"/>
            <dbReference type="Rhea" id="RHEA-COMP:13543"/>
            <dbReference type="Rhea" id="RHEA-COMP:13544"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.88"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="11">
            <dbReference type="Rhea" id="RHEA:53378"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>5,6-dihydrouridine(17) in tRNA + NAD(+) = uridine(17) in tRNA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53372"/>
            <dbReference type="Rhea" id="RHEA-COMP:13541"/>
            <dbReference type="Rhea" id="RHEA-COMP:13542"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.88"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="10">
            <dbReference type="Rhea" id="RHEA:53374"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>5,6-dihydrouridine(17) in tRNA + NADP(+) = uridine(17) in tRNA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53368"/>
            <dbReference type="Rhea" id="RHEA-COMP:13541"/>
            <dbReference type="Rhea" id="RHEA-COMP:13542"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.88"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="13">
            <dbReference type="Rhea" id="RHEA:53370"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the Dus family. Dus1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.88" evidence="9"/>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YHN6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017150">
        <property type="term" value="F:tRNA dihydrouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02801">
        <property type="entry name" value="DUS_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000162">
        <property type="entry name" value="tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035587">
        <property type="entry name" value="DUS-like_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018517">
        <property type="entry name" value="tRNA_hU_synthase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11082">
        <property type="entry name" value="TRNA-DIHYDROURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11082:SF5">
        <property type="entry name" value="TRNA-DIHYDROURIDINE(16_17) SYNTHASE [NAD(P)(+)]-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01207">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01136">
        <property type="entry name" value="UPF0034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="DUS-like FMN-binding" evidence="14">
        <location>
            <begin position="96"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9670650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="424" mass="48100" checksum="2182EB770A98548A" modified="2024-01-24" version="1">MKLNSWPFSPLFTSLKHLSRSIHTMASSISETLTLTLPAGSPQDLNLDEHLCSEQQEHQDCQSSSADWPSRCLTGESRIERAWAHWKKLGQPKLIVAPMVDNSELPFRMLCRKYGAQAAYTPMLHSRIFSENEKYRSQEFTTCKEDRPLFVQFCANDPDTLLEAAQRVEPYCDYVDINLGCPQRIARRGNYGAFLMDNLPLVKSLVEKLALNLHVPVSCKIRVFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKDGRKFRADWNAIKAVKNAVNIPVLANGDIRHMDDVQRCLEETGADGVLSAETLLENPALFAGFQTAEWALGGEEGNKDGELDQANLLVEYLKLCEKYPVPWRMIRSHVHKLLGEWFRIQPQVRDDLNSQSKLTFEFLYNMVDQLRELGVRIPLYTKDTPAGRTSNGIAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YIJ5</accession>
    <name>A0AA38YIJ5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Aldehyde dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_027186</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>an aldehyde + NAD(+) + H2O = a carboxylate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:16185"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:29067"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.2.1.3"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9671075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YIJ5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006081">
        <property type="term" value="P:aldehyde metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009737">
        <property type="term" value="P:response to abscisic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009414">
        <property type="term" value="P:response to water deprivation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07137">
        <property type="entry name" value="ALDH_F3FHI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000003">
        <property type="entry name" value="Aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000004">
        <property type="entry name" value="Aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012394">
        <property type="entry name" value="Aldehyde_DH_NAD(P)"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43570">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43570:SF25">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE FAMILY 3 MEMBER I1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036492">
        <property type="entry name" value="ALDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="2 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="7">
        <location>
            <begin position="75"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="281"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00049194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR036492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR036492-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9671075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="549" mass="60725" checksum="7842B14D06BFA59F" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YJ14</accession>
    <name>A0AA38YJ14_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_025150</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YJ14"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22160">
        <property type="entry name" value="F-box_AtFBL13-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053781">
        <property type="entry name" value="F-box_AtFBL13-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006566">
        <property type="entry name" value="FBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050232">
        <property type="entry name" value="FBL13/AtMIF1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055411">
        <property type="entry name" value="LRR_FXL15/At3g58940/PEG3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31900:SF34">
        <property type="entry name" value="EMB|CAB62440.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31900">
        <property type="entry name" value="F-BOX/RNI SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08387">
        <property type="entry name" value="FBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24758">
        <property type="entry name" value="LRR_At5g56370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="2"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9671316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="450" mass="51929" checksum="75779C468CBBE289" modified="2024-01-24" version="1">MEDYFSFLPDPIIVPIISYLTFRDAARTSVLSQRWRHLWRSTKNIEFDETLFMREVESREERELCRRDFIYFVRQWILDYQEPVVDMFRLAFSNPKQYQLEIENCVRFAIAHGVKRLDLDFCDPAWGEYNSDEPPPSFALPSCVYRNRVLESLRLSSCTFHVPRINTFTALKDLSIGWSELTSFALSALLVNCPLLESLSLKKCWNLDDPVISGPKLKLRSLVMDKCAFIYRFTVGPPLLRSLSYSGAVSEFVFLNQPPVEKVELDFGLEPDIDYEGTYGDVIRELLGDLKCTTLTVCSFMLQHLQGLTHLTVRTAVHEYESYGFAFLLSSCRRLQILTIEIVPGRIFPDYAPPSELRGNNVFWRQLEPVPHCMIETLRIVEMKGFGGEPNELQLVKYLLRHGHVMGTLNIIVKKQMGHGDADTESNSRLWAEMLQYYARASSGLQILIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YJI0</accession>
    <name>A0AA38YJI0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein yippee-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_025344</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the yippee family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YJI0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034751">
        <property type="entry name" value="Yippee"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004910">
        <property type="entry name" value="Yippee/Mis18/Cereblon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039058">
        <property type="entry name" value="Yippee_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13848">
        <property type="entry name" value="PROTEIN YIPPEE-LIKE CG15309-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03226">
        <property type="entry name" value="Yippee-Mis18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51792">
        <property type="entry name" value="YIPPEE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Yippee" evidence="5">
        <location>
            <begin position="12"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU110713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9671583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="127" mass="14433" checksum="B977AC46341ECB0A" modified="2024-01-24" version="1">MGRVFVVELEGRAYECKFCKTHLALVDDLVSRAFHCRRGKAYLFNNAVNITVGAQEERMMISGMHTVADIFCCCCGQIVGWKYEMAHDKTQKYKEGKFVLERGRIADGVDSEFYIDTRANMSDAEDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YKU4</accession>
    <name>A0AA38YKU4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_025778</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YKU4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31208:SF2">
        <property type="entry name" value="DOMAIN-CONTAINING PROTEIN, PUTATIVE, EXPRESSED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31208">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="2">
        <location>
            <begin position="35"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="285"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="396"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="302"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="323"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="401"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9672297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="438" mass="47529" checksum="547F232EC21A04B9" modified="2024-01-24" version="1">MDSPQSVVSPFKSSPVSIEPEKQKSDLFVRNPGCLSGGIEVHRKESGMVCNMDGFVGSLEVYIHQARDIHNICIYHKQDVYAKLCLTSDPENAVSTKIINGGGQNPVFNDNLRLSVRTTESSLKCEIWMLSRVKNYLEDQLLGFALVPLSEVLLNNGKLEKEFSLSSTDLFHSPAGFVRLSLSYSGASPEVMAIPALPTALATNEASQDTEISESMPSDYDAIEFPDPKIMNENQLMVSEYYGIPCTNLDSRSSEGTLDDESHRSSEKIGVQVVESFSEGTIESVQLPKLDSPPSSVSTNGSPSASLPASSVSSDASGTSKSPNEEHVSPPKEKNADSGDGESDSSGGAPCNTFAKPAVTVNIEPEQKMVQQDIVDMYMKSMQQFTESLAKMKLPMDIETEPTNSGNSSSDQKLQAAKNNNNNNNNNSRVFYGSRAFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YMW8</accession>
    <name>A0AA38YMW8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cytochrome P450 87A3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_023117</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YMW8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016132">
        <property type="term" value="P:brassinosteroid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010268">
        <property type="term" value="P:brassinosteroid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016125">
        <property type="term" value="P:sterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11043">
        <property type="entry name" value="CYP90-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000020">
        <property type="entry name" value="Cytochrome P450 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286:SF90">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286">
        <property type="entry name" value="CYTOCHROME P450 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12" id="KW-0349">Heme</keyword>
    <keyword evidence="9 12" id="KW-0408">Iron</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="272"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="12">
        <location>
            <position position="418"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9673337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="471" mass="53380" checksum="9C46DC7C5FA2C352" modified="2024-01-24" version="1">MYGSVWLGVVTLFIAGITHWVYKWRNPKCNGKLPPGSMGLPLIGETIQFFIPSKSLDVPNFIKKRMKKYGPLFCTNLVGRPVVVSSDPDFNYYIFQQEGKLVELWYLDSFAKLVGLDPSSNAATGYVHKYARNLILAYLGTEVLKDKLLYRAEGLIRTRLHDWSKLPAFEIKACLSSMVFDLTAMEVLSDDFKKMGAHFSEKFANILQALFSFPLNIPGTTFRECLKNQKEAKRLIRDLLKERKVSFESCKGDLLDKLVDDAKIEKFLSDEYIVIFVFVILLASFETIPLATTLALKCLTEHPLAMQELIEEHEAILKNKENPNSGISWKEYKSMTFTHQVINETLRLANIVPGILRRAIKDIQVNAGWIILLYPAALQLDPNTFTDPLTFNPWRWKDIGAGVRAKNFMPFGCSSRSCAGAEFTKALMATFLHVLVTNYRLTKIKGGKIARSPSLIFENGFHINISKKHGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YNE0</accession>
    <name>A0AA38YNE0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Aldehyde dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_023259</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>an aldehyde + NAD(+) + H2O = a carboxylate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:16185"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:29067"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.2.1.3"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YNE0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006081">
        <property type="term" value="P:aldehyde metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009737">
        <property type="term" value="P:response to abscisic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009414">
        <property type="term" value="P:response to water deprivation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000003">
        <property type="entry name" value="Aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000004">
        <property type="entry name" value="Aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012394">
        <property type="entry name" value="Aldehyde_DH_NAD(P)"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43570">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43570:SF16">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE TYPE III, ISOFORM Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036492">
        <property type="entry name" value="ALDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="2 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="7">
        <location>
            <begin position="31"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="222"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00049194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR036492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR036492-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9673588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="488" mass="52898" checksum="5AAE78F2ED8543D6" modified="2024-01-24" version="1">MAEDSETKKVFDAEAAGSLMKELRGTYASGKTRSYEWRVAQLKNVMKIVDDHEKDILDAIRADLSKPEQESYIAEISIVKSSCTRALGELNRWMKPEKVKTSITTFPSSAEIVSEPLGVVLIIGAWNFPLLLALDPAIGAIAAGNAVVLKPSELCPATSSLIAKLVGKYLDSSCIKVVEGSVAETSALLEQKWDKIFFTGSGRVGRIVMAAAAKHLTPVALELGGKCPVVVDSDINLPVAVRRIAGGKWASNNGQACIAPDYIITTKDFAPKLIDALKHELEAWYGKDPLESKDLAHIVNSNHFARLVKLLDDDKVSGKIIHGGQRDKANLKFAPTILLDVPEDSLVMNDEIFGPLLPILTVDKLEDSFDMITSRGKPLAAYLFTNNKKLKEKFVKTVSAGGLVINDTVLHFAEKTLPFGGVGESGIGSYHGKFSYEAFSHRKSVLYRGFAGDASARYPPYSDRKLKLLKALLSGSVVGVILALIGWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YPX9</accession>
    <name>A0AA38YPX9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_023779</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YPX9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="29"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9674435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="106" mass="12245" checksum="4AA7899E44B21B8A" modified="2024-01-24" version="1">MPRNRNHTPEIRCIHFPSTVESKSTSVPYTKQQLQEFFKQHDKDGDKLLSKEELKKAFDDLHSRNPGFRAWRAMGKADADGDQHISQQEMEALLDYAVGKKYPVKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YQB1</accession>
    <name>A0AA38YQB1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein LIKE COV 1-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_023936</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YQB1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007462">
        <property type="entry name" value="COV1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31876">
        <property type="entry name" value="COV-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31876:SF20">
        <property type="entry name" value="PROTEIN LIKE COV 3-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04367">
        <property type="entry name" value="DUF502"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="99"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="27"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9674703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="264" mass="28912" checksum="66657906F6382D83" modified="2024-01-24" version="1">MGSRERDRDLELLIPVTATTDNVASKSSPSSPSSSSSHHHSGREAFSKVIRSWASKKFMSGCVILLPIAITFYITWGFIHFVDGFFSPVYNQLGINVFGLGFITSITFIFLVGVFMSSWLGASLLSLGEWFIKKMPLVSYIYSASKQISAAISPDQSSNAFKEVAIIRHPRIGEYAFGFITNTVLLQRNAGEEELCCIYVPSNHLYIGDVFLISSMDILRPNLSVREGIEIVISGGMSVPQILTTIDAQAIPASRIGNFVAAKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YSJ0</accession>
    <name>A0AA38YSJ0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Deoxynucleoside kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_024658</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YSJ0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019136">
        <property type="term" value="F:deoxynucleoside kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01673">
        <property type="entry name" value="dNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050566">
        <property type="entry name" value="Deoxyribonucleoside_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031314">
        <property type="entry name" value="DNK_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10513:SF35">
        <property type="entry name" value="DEOXYADENOSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10513">
        <property type="entry name" value="DEOXYNUCLEOSIDE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01712">
        <property type="entry name" value="dNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Deoxynucleoside kinase" evidence="2">
        <location>
            <begin position="251"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9675815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="568" mass="63320" checksum="351B35D1E7F42610" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YT22</accession>
    <name>A0AA38YT22_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_024919</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9676155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YT22"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008353">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000624">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF384">
        <property type="entry name" value="PROTEIN KINASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="1"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="339"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="447"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="339"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="379"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9676155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="540" mass="58715" checksum="59DE5029169CCEF8" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YVV4</accession>
    <name>A0AA38YVV4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 18">Peroxidase</fullName>
            <ecNumber evidence="4 18">1.11.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PVL29_022564</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 18">
            <text>2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:56136"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:139520"/>
            <dbReference type="ChEBI" id="CHEBI:139521"/>
            <dbReference type="EC" id="1.11.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 18">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="15 18">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 18">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="15 18">Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="18">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peroxidase family. Ascorbate peroxidase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.7" evidence="4 18"/>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YVV4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140825">
        <property type="term" value="F:lactoperoxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00693">
        <property type="entry name" value="secretory_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.420.10:FF:000001">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.520.10:FF:000008">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.420.10">
        <property type="entry name" value="Peroxidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000823">
        <property type="entry name" value="Peroxidase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019794">
        <property type="entry name" value="Peroxidases_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019793">
        <property type="entry name" value="Peroxidases_heam-ligand_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033905">
        <property type="entry name" value="Secretory_peroxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31517">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31517:SF17">
        <property type="entry name" value="PEROXIDASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00458">
        <property type="entry name" value="PEROXIDASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00461">
        <property type="entry name" value="PLPEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00435">
        <property type="entry name" value="PEROXIDASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00436">
        <property type="entry name" value="PEROXIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50873">
        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 18" id="KW-0106">Calcium</keyword>
    <keyword evidence="11 17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 18" id="KW-0349">Heme</keyword>
    <keyword evidence="12 18" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="10 15 18" id="KW-0408">Iron</keyword>
    <keyword evidence="8 15 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 18" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6 18" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 18" id="KW-0964">Secreted</keyword>
    <keyword evidence="18" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041488898" description="Peroxidase" evidence="18">
        <location>
            <begin position="18"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="Plant heme peroxidase family profile" evidence="19">
        <location>
            <begin position="21"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="62"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="15">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="16">
        <location>
            <position position="58"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="31"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="64"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="118"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="197"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU362060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAJ9677646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="327" mass="36041" checksum="3A0189EA83E4084A" modified="2024-01-24" version="1">MAFSFLVLLLFLSGISSSESKLNVNYYQKSCPSFERIMQDAITSKQINSPTTAAGTLRLFFHDCMVDGCDASVLISSNAFNTAERDAEINLSLPGDAFDLVVRAKTSLELTCPGIVSCADILALATRNLVTMVGGPYYDVQLGRKDGLVSQASRVEGNLPRANMTMDQLIAMFNAKGFSIQEMVALSGAHTIGFSHCKEFSNRIFNYSSTSDIDPAFNPKFAQALRKVCANYQRDTAMSAFNDVMTPSKFDNMYYQNLPRGLGLLSSDNVLVTDPRTKPFVELYATNQKAFFNDFAHAMEKLSVSGIKTGRKGEVRRRCDAFNDIKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YXQ9</accession>
    <name>A0AA38YXQ9_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_020609</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YXQ9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9678467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="163" mass="18154" checksum="1875333279A46704" modified="2024-01-24" version="1">MSIVQSDTTDLMMKNCRMIDNEEGDDSNKEEDKAPSEVTKQEQESLIEKATEECEKKLQEVEKSETPVEEALVSSTKQTFPEVEKSKIPVEEPPVTSTKQKLPEVEKSETPVEEALVSFTEQKLPEVEKSETPVEEAPVSFSEKKIDIEAAVAAAEVEPTPNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YYT2</accession>
    <name>A0AA38YYT2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">phosphopyruvate hydratase</fullName>
            <ecNumber evidence="3">4.2.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_021130</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="9">Mg(2+) is required for catalysis and for stabilizing the dimer.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the enolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.11" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YYT2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000015">
        <property type="term" value="C:phosphopyruvate hydratase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004634">
        <property type="term" value="F:phosphopyruvate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03313">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.10:FF:000001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.120:FF:000002">
        <property type="entry name" value="Enolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="entry name" value="Enolase-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="entry name" value="Enolase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00318">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000941">
        <property type="entry name" value="Enolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020810">
        <property type="entry name" value="Enolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020809">
        <property type="entry name" value="Enolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020811">
        <property type="entry name" value="Enolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01060">
        <property type="entry name" value="eno"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902:SF46">
        <property type="entry name" value="ENOLASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00113">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03952">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001400">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00148">
        <property type="entry name" value="ENOLASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00002">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00178">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01192">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01193">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="Enolase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="Enolase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00164">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enolase N-terminal" evidence="11">
        <location>
            <begin position="4"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Enolase C-terminal TIM barrel" evidence="10">
        <location>
            <begin position="147"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="215"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="353"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="250"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="301"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="301"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="328"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="328"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="380"/>
            <end position="383"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="404"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9679100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="445" mass="48099" checksum="4DA529B505918055" modified="2024-01-24" version="1">MATIKVIKARQIFDSRGNPTVEVDVTLSDGTFARAAVPSGASTGIYEALELRDGGSDFLGKGVLKAVENVNAIIAPALIGKDPTEQVKIDNFMVQQLDGTVNEWGWCKQKLGANAILAVSLAVCKAGAMVNKIPLYKHIANLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPLGASSFKEAMKMGVEVYHHLKSVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKTAIAKAGYTGKVVIGMDVAASEFYDSKDKTYDLNFKEENNDGSQKISGDSLKNVYKSFVNDYPIVSIEDPFDQDDWEHYAKMTSEIGEKVQIVGDDLLVTNPKRVEKAIKEKSCNALLLKVNQIGSVTESIEAVKMSKRAGWGVMASHRSGETEDTFIADLSVGLATGQIKTGAPCRSERLAKYNQLLRIEEELGSAAVYAGAKFRAPVEPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YZN1</accession>
    <name>A0AA38YZN1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">chitinase</fullName>
            <ecNumber evidence="2">3.2.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_021545</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1-&gt;4)-beta-linkages in chitin and chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.14"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the glycosyl hydrolase 18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.14" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YZN1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008843">
        <property type="term" value="F:endochitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02877">
        <property type="entry name" value="GH18_hevamine_XipI_class_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000015">
        <property type="entry name" value="Acidic endochitinase SE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045321">
        <property type="entry name" value="Cts1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001579">
        <property type="entry name" value="Glyco_hydro_18_chit_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050542">
        <property type="entry name" value="Glycosyl_Hydrlase18_Chitinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45708:SF21">
        <property type="entry name" value="ACIDIC ENDOCHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45708">
        <property type="entry name" value="ENDOCHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01095">
        <property type="entry name" value="GH18_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0146">Chitin degradation</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041397188" description="chitinase" evidence="11">
        <location>
            <begin position="26"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="GH18" evidence="12">
        <location>
            <begin position="26"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9679651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="296" mass="31964" checksum="F82FEC62884C179B" modified="2024-01-24" version="1">MARTPQSTALLISLSVLAFLQTSYAGGIAIYWAQNGIEGTLTETCNTGKYSYVNIAFLNKFGNGRTPEINLGGHCDPASNGCTSLSTDIRNCQNRGIKVMLSIGGRDGSYSLSSSKDAQKVADYLWNNFLGGQSSSRPLGDAVLDGIDFDIELGSTKYWDNLARALSGFSKRGRKVYLTAAPQCAFPDRFLGAALNTGLFDHVWVQFYNNAPCQYTTGNTNKLLNSWNRWTSSINSRIFLGLPASSAAAGGGFIPANVLTSQILPVIKRSPKYGGVMLWSYYDDQSGYASTIKSSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z0Q7</accession>
    <name>A0AA38Z0Q7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Amidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_019452</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z0Q7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000120">
        <property type="entry name" value="Amidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895:SF73">
        <property type="entry name" value="AMIDASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895">
        <property type="entry name" value="TRANSAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="1">
        <location>
            <begin position="172"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9680164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="625" mass="68624" checksum="82BEF5E1579881D6" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z1X3</accession>
    <name>A0AA38Z1X3_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_019799</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z1X3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008480">
        <property type="entry name" value="DUF761_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34059:SF6">
        <property type="entry name" value="DUF4408 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34059">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05553">
        <property type="entry name" value="DUF761"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="69"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="157"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="231"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="247"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="303"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9680593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="429" mass="47418" checksum="7C547658A9D286E5" modified="2024-01-24" version="1">MGLRIHVGLVRRVIQTWDAQYFRGETMVVVAKESYALDQWGKPESSVDDKPLGLPVRSLKSRIKDTDSTESVSGSRSKSSSKTGSSKSSGKVRNGKFGKLGPLKLEEQLKDTVALPSPIPWRSRSGRMEMRDEADSFHSHSHSMPPSFEEPEFEQLQSRSFRSSTPYSSQTSSVSSSPGKLSPSSPASSELGNSKMEELGRRKSPYGSSTATSPSPPGQMNGKAPLDMSRSHQFFNGSASEMNAQRSFEDERKELGRSRREDQVNSKEWQLGSMKSAMKPGPPNKHLSRGRSVRTFRASELTSEARKVAEEGGNRIDDSVGKTYIKAEEGPMEKSDMKSKVLGNTSYVTKPPFSKHPKGEIQDSEDLEGEAHNFGTSSDEEVASNIVGEEEPVPHEVDKKAGEFIAKFKEQIRLQKMASIRGHTRETRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z1X9</accession>
    <name>A0AA38Z1X9_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_020006</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z1X9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000138">
        <property type="entry name" value="Os10g0167200 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47950">
        <property type="entry name" value="CYTOCHROME P450, FAMILY 76, SUBFAMILY C, POLYPEPTIDE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47950:SF44">
        <property type="entry name" value="CYTOCHROME P450, FAMILY 76, SUBFAMILY C, POLYPEPTIDE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0349">Heme</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="2">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9680866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="174" mass="19603" checksum="864735DBA08F0921" modified="2024-01-24" version="1">MAELLNNPKSMAKARSELGKVVGKEKIVEESDISKLPYLQAVVKETFRFHPPVPFLILGYAVPKNAHVLVIGWAIGRDSTISSNPNLFVLERFLECEIDVKSRDFQLSPFGAGRRICSGLLLGHRMVHLMLASLLHSFDWKLEDGLKPEDMDMTEKFGFTFRKAQPLQAIPIKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z292</accession>
    <name>A0AA38Z292_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribosome biogenesis regulatory protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_020112</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in ribosomal large subunit assembly.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the RRS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9681048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z292"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007023">
        <property type="entry name" value="Ribosom_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17602">
        <property type="entry name" value="RIBOSOME BIOGENESIS REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17602:SF4">
        <property type="entry name" value="RIBOSOME BIOGENESIS REGULATORY PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04939">
        <property type="entry name" value="RRS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="58"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="138"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="217"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="284"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="221"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="305"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9681048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="327" mass="36715" checksum="C098DE32EEA426A4" modified="2024-01-24" version="1">MGTQEHQIDLGHLLAFDPLHHFPSPPSSREELVKECLQKGTELVQSIADTLFNLPSTEDIDGPLVKLPPPTTRLPREKHLPKPKPPTKWELFAKAKGIKNRKKDKIVYDEQTGTWKRRHGYDRVNDDEDIPIIEAKMTDEAGEDPFAKRQAEKKKRVEKQEKNRLQNLKQAAKVGALPSHIQLAATALPITGTQTAPKKVSKNELEDVASMAATATASGGKFDKKLPGEKPEKHKGKYRKFLPVVEGTGIGSLEKQQTEKILNKLISKNSHEILNVDRAINMYNVKKEKKRKNRQGKDSSTSSKLKPKKQLQKKSLKKGTSKKGKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z552</accession>
    <name>A0AA38Z552_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_018390</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057135">
        <property type="entry name" value="At4g27190-like_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050905">
        <property type="entry name" value="Plant_NBS-LRR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463:SF198">
        <property type="entry name" value="RPP4C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23247">
        <property type="entry name" value="LRR_RPS2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="169"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="27"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9682460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1802" mass="203877" checksum="E9BB58F6C3E6A513" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z5B6</accession>
    <name>A0AA38Z5B6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">50S ribosomal protein 6, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_018563</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z5B6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020526">
        <property type="entry name" value="Ribosomal_cL38"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36798">
        <property type="entry name" value="50S RIBOSOMAL PROTEIN 6, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36798:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN CL38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17257">
        <property type="entry name" value="DUF5323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9682665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="89" mass="9541" checksum="F6A27D73ADBE726C" modified="2024-01-24" version="1">MTASFGMRKAAPRLGGSAAVIECSSRPQKKATSHHMKTHPHKTQPWDIKRPPTVYASLPPLPPKWTIVSSYANASDAAVPPPTAQPATE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z5N8</accession>
    <name>A0AA38Z5N8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_018733</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z5N8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009736">
        <property type="term" value="P:cytokinin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009740">
        <property type="term" value="P:gibberellic acid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010090">
        <property type="term" value="P:trichome morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002829">
        <property type="entry name" value="Zinc finger protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044299">
        <property type="entry name" value="GIS3/ZFP5/ZFP6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46353">
        <property type="entry name" value="ZINC FINGER PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46353:SF13">
        <property type="entry name" value="ZINC FINGER PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="52"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9682861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="211" mass="23260" checksum="F93D7EFA17E7CE3E" modified="2024-01-24" version="1">MADIHYQTKPNNATMGLKLFGFNISEVEELESGKSPSGSPESEIFQADGRRYECQYCCREFANSQALGGHQNAHKKERQQLKRAKLQASARSFPDSFTRNPMVSAFMPPPHLFAPPSAPPQSYPWAYMPRAGAAPSFRAPQRGFMIKCGSEAAPEAYAGNERETPVRATLYREVQAHAGVVPELLRRGDDGPRFEKGLGLDLHLSLGPAKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z776</accession>
    <name>A0AA38Z776_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_018935</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z776"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009873">
        <property type="term" value="P:ethylene-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000001">
        <property type="entry name" value="Ethylene-responsive transcription factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31677">
        <property type="entry name" value="AP2 DOMAIN CLASS TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31677:SF118">
        <property type="entry name" value="OS04G0399800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00367">
        <property type="entry name" value="ETHRSPELEMNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0936">Ethylene signaling pathway</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="8">
        <location>
            <begin position="21"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9683144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="258" mass="28825" checksum="85FC222A0EBDE8ED" modified="2024-01-24" version="1">MNPSTSKSKKKQTQQQETSGRFLGVRRRPWGRYAAEIRDPSTKERHWLGTFDTAEEAALAYDRAARSMRGSRARTNFVYSDMPPGSSVTSIISPDESLPDFSSHFVPPTTQPNPNPNLFFNPTECHQFPFQGQFPTETNAWDYSNLYQEQPLVDNMNGTTTNNQFYNATDLPPFPSQVSDQLPSSGFEMEQATWGDSTNLAGFSEQRTSPNEFDSVGGGSYLGFDSTQYVHSPLFGRMPPVSDTPPDGFDLGSSSYFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z7G0</accession>
    <name>A0AA38Z7G0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_019336</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z7G0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.150.80:FF:000006">
        <property type="entry name" value="NAC domain-containing protein 100-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.80">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003441">
        <property type="entry name" value="NAC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036093">
        <property type="entry name" value="NAC_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31744:SF219">
        <property type="entry name" value="NAC DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31744">
        <property type="entry name" value="PROTEIN CUP-SHAPED COTYLEDON 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02365">
        <property type="entry name" value="NAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101941">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51005">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NAC" evidence="6">
        <location>
            <begin position="16"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="186"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="190"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9683719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="363" mass="41201" checksum="C0E378163E11E5AE" modified="2024-01-24" version="1">MEKAPDQSKDDEMMELPPGFRFHPTDEELITHYLSQKVLNSGFYAVAIGEVDLNKCEPWDLPWKAKMGEKEWYFFCVRDRKYPTGLRTNRATDAGYWKATGKDKEIYKMKTLVGMKKTLVFYKGRAPKGEKTNWVMHEYRLEGKHSMYSLPKTTKNEWVICRVFQKSSGGKKTHISGLVRMDPYSDELRSSSQLPPLTDSSPFSSETRTVGDSSHVTCFSNQMEDRKPQEDMIDSFNNPLLAASSSSSNPSDISPASLLFSKFPFPNSFHTPQIPPNIGNYQFPDSFLIPDQSILRILLENQGSDMKPSSKTEFSQETGLSTDINTDISSVVSTHEMVQRSFEDQNDPSTAAGPVDIDCLWNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z7J3</accession>
    <name>A0AA38Z7J3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_019160</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z7J3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000530">
        <property type="entry name" value="probable receptor-like protein kinase At5g59700"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000608">
        <property type="entry name" value="Serine/threonine kinase protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051564">
        <property type="entry name" value="LRR_receptor-like_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043891">
        <property type="entry name" value="SPARK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48055">
        <property type="entry name" value="LEUCINE-RICH REPEAT RECEPTOR PROTEIN KINASE EMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48055:SF26">
        <property type="entry name" value="TRANSFERASE, PROTEIN KINASE RLK-PELLE-URK-2 FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19160">
        <property type="entry name" value="SPARK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="251"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="314"/>
            <end position="582"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9683458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="622" mass="69290" checksum="804279703BB06840" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZAV1</accession>
    <name>A0AA38ZAV1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Disease resistance RPP13-like protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_017507</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056789">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766:SF51">
        <property type="entry name" value="DISEASE RESISTANCE RPP13-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766">
        <property type="entry name" value="PLANT BROAD-SPECTRUM MILDEW RESISTANCE PROTEIN RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25019">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0611">Plant defense</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="7">
        <location>
            <begin position="11"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="6">
        <location>
            <begin position="180"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="8">
        <location>
            <begin position="438"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="domain" description="R13L1/DRL21-like LRR repeat region" evidence="9">
        <location>
            <begin position="697"/>
            <end position="821"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF25019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9685489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1324" mass="150891" checksum="8C93531E8A6DE98F" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZF55</accession>
    <name>A0AA38ZF55_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FLZ-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_013895</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FLZ family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9687886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZF55"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044604">
        <property type="entry name" value="FLZ12/13/14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007650">
        <property type="entry name" value="Zf-FLZ_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47208:SF5">
        <property type="entry name" value="FCS-LIKE ZINC FINGER 12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47208">
        <property type="entry name" value="OS02G0174800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04570">
        <property type="entry name" value="zf-FLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51795">
        <property type="entry name" value="ZF_FLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FLZ-type" evidence="5">
        <location>
            <begin position="162"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="FLZ-type" evidence="4">
        <location>
            <begin position="162"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9687886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="235" mass="25727" checksum="A15E709A86422228" modified="2024-01-24" version="1">MLGKTSRPAIGKLAGSLISGNRTAILDVATSPRSPLDMKIQSPRGQKNYDHFGGVGLGIVAALDKSGNCGGEILAKYAVSGSNLGRSGPVPVKSGKNCGRLKVGFEELEMDSLEDYTYVTCHRPNNKSFTRVYFDGHETTAFHKHESPARFSNDMPAFPTSDFLSSCHLCRKKLHGKDIYMYRGEKAFCSNECRSRQIVMDERKEQCRSEASRSADVSSSPYTRGQIFSTGILAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZG94</accession>
    <name>A0AA38ZG94_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ornithine decarboxylase</fullName>
            <ecNumber evidence="6">4.1.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_014268</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-ornithine + H(+) = putrescine + CO2</text>
            <dbReference type="Rhea" id="RHEA:22964"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:326268"/>
            <dbReference type="EC" id="4.1.1.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Amine and polyamine biosynthesis; putrescine biosynthesis via L-ornithine pathway; putrescine from L-ornithine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer. Only the dimer is catalytically active, as the active sites are constructed of residues from both monomers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Orn/Lys/Arg decarboxylase class-II family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.17" evidence="6"/>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZG94"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004586">
        <property type="term" value="F:ornithine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033387">
        <property type="term" value="P:putrescine biosynthetic process from arginine, via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00622">
        <property type="entry name" value="PLPDE_III_ODC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.10:FF:000005">
        <property type="entry name" value="Ornithine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="entry name" value="Alanine racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.37.10">
        <property type="entry name" value="Lyase, Ornithine Decarboxylase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009006">
        <property type="entry name" value="Ala_racemase/Decarboxylase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022644">
        <property type="entry name" value="De-COase2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022653">
        <property type="entry name" value="De-COase2_pyr-phos_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000183">
        <property type="entry name" value="Orn/DAP/Arg_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002433">
        <property type="entry name" value="Orn_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11482">
        <property type="entry name" value="ARGININE/DIAMINOPIMELATE/ORNITHINE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11482:SF6">
        <property type="entry name" value="ORNITHINE DECARBOXYLASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02784">
        <property type="entry name" value="Orn_Arg_deC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01179">
        <property type="entry name" value="ODADCRBXLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01182">
        <property type="entry name" value="ORNDCRBXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50621">
        <property type="entry name" value="Alanine racemase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="PLP-binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00878">
        <property type="entry name" value="ODR_DC_2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 9" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Orn/DAP/Arg decarboxylase 2 N-terminal" evidence="10">
        <location>
            <begin position="52"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="9">
        <location>
            <position position="356"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="9">
        <location>
            <position position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR600183-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9688521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="411" mass="45234" checksum="DA6293831164D1E6" modified="2024-01-24" version="1">MSASASLQLRLAATQLRNWDVHRVSGDGFTALIRSISQKQKDREPFYILDLGAVVRLMDMWKQALPDVVPYYAVKCNSQPPLITALASLGANFDCASRAEIETVMALGVGAQQIVYANPCKGQSHIKYAASVGVNLTTFDSMQEIDKIKMWHEKCDLLLRIKAPNDEKGSWRPLGAKFGALPEEVVPLLQHANAAGLRVIGVSFHVGSKVNDPQLYRGAIASARGVFDAAVQLKLPPMHVLNIGGGFQESLTFHEIAAVIKEAINDYFPSSETSEDLKIMAEPGRFFAETAFTLVSNIIGKRARGEKREYWIDDGIYGSFNLPAYDKSSMMVKPLLGGSEWMNKAKFCSTVFGPTCDSMDMVVAESQLPELHMNDVLVFYNMGAYTASAGTRFNGFDISSISTFLTYVYRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZGZ8</accession>
    <name>A0AA38ZGZ8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_014409</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZGZ8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000041">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000213">
        <property type="entry name" value="Tyrosine-sulfated glycopeptide receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025875">
        <property type="entry name" value="Leu-rich_rpt_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051502">
        <property type="entry name" value="RLP_Defense_Trigger"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48062:SF21">
        <property type="entry name" value="RECEPTOR-LIKE PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48062">
        <property type="entry name" value="RECEPTOR-LIKE PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12799">
        <property type="entry name" value="LRR_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="892"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="13">
        <location>
            <begin position="29"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9688742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="937" mass="104521" checksum="D0E32F4295673ED7" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZH54</accession>
    <name>A0AA38ZH54_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_014564</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZH54"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080147">
        <property type="term" value="P:root hair cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000017">
        <property type="entry name" value="Transcription factor bHLH66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045843">
        <property type="entry name" value="IND-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16223:SF378">
        <property type="entry name" value="TRANSCRIPTION FACTOR BHLH10-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16223">
        <property type="entry name" value="TRANSCRIPTION FACTOR BHLH83-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="7">
        <location>
            <begin position="292"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="26"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="90"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="198"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="239"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="264"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="377"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="471"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="28"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="52"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="96"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="268"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9688971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="518" mass="54330" checksum="DFA1810F73F1E76D" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZJ82</accession>
    <name>A0AA38ZJ82_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phototropic-responsive NPH3 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_012551</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NPH3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9689942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZJ82"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18312">
        <property type="entry name" value="BTB_POZ_NPY3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043454">
        <property type="entry name" value="NPH3/RPT2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027356">
        <property type="entry name" value="NPH3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32370">
        <property type="entry name" value="OS12G0117600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03000">
        <property type="entry name" value="NPH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51649">
        <property type="entry name" value="NPH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="BTB" evidence="5">
        <location>
            <begin position="28"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="NPH3" evidence="6">
        <location>
            <begin position="199"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="481"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="492"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="507"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="528"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="549"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="582"/>
            <end position="593"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9689942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="593" mass="67507" checksum="1783341A160E4055" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZKU5</accession>
    <name>A0AA38ZKU5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">THH1/TOM1/TOM3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_013213</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the plant tobamovirus multiplication TOM1 protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZKU5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040226">
        <property type="entry name" value="THH1/TOM1/TOM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009457">
        <property type="entry name" value="THH1/TOM1/TOM3_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31142:SF4">
        <property type="entry name" value="OS01G0751300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31142">
        <property type="entry name" value="TOBAMOVIRUS MULTIPLICATION PROTEIN 1-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06454">
        <property type="entry name" value="THH1_TOM1-3_dom"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="53"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="83"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="166"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="200"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="244"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="285"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="THH1/TOM1/TOM3" evidence="8">
        <location>
            <begin position="16"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="THH1/TOM1/TOM3" evidence="8">
        <location>
            <begin position="165"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF06454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9690946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="370" mass="41307" checksum="429766BD1658DC61" modified="2024-01-24" version="1">MFELRDGSCFPRVLAAVNVALACIDGVVALLAFSQLIRIHSRSRQLGWTRQKVFHLMIGSSNVGYFLYFVLTHVATCKGWLCWSNSCGFVLMACPKILLLAAFLLLLSFWVDLCHQPDDEDDEDEESSSQEALLEKTLNLPSSSNKNSHRKCCSLRAIHIGSRQKIVILVIVLIFVLMVTFAVLIWIGMGKNLIDSSVVARVYVDLFAIAILLLGGALACYGLMLCLKMRKVRSERASSEMSKVAGLAVVSVICFTSSAFVDLFTNIPMLYHWHPQPVNGVCTSLLLILYYFIGSSVPSAFVLWVMRELPPSITTDVYVESRPVTFISDSSESVNHPQRWSTATSSQNQHYHVLFLQFLPLLYVGSPNMH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZL88</accession>
    <name>A0AA38ZL88_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_012851</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the AB hydrolase superfamily. Epoxide hydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZL88"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43329">
        <property type="entry name" value="EPOXIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="27"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9690403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="310" mass="34895" checksum="339100FAF53FDF95" modified="2024-01-24" version="1">MDQIQHNFIDVNGLKLHVAEIGSGPTTVVFLHGFPEIWYSWRHQMIAVAKAGFRAIAPDHRGYGLSEPPPEPEKASFSDLLADLVGILDFLGIDKVFLVAKDFGGRPAYLLSVFHPERVLGVVTVGAPFPPGPSVYIKDLPEGFYILRWQKPGRAEADFGRFDAKTIVRNIYILFSRPEIPIAAENQEVMDMVDPSTPLPPWFTEEDLTAYGALYEKSGFRTALQVPHRSINEQFNITDPKVEVPMLLIMGEKDFSFKFPGKEEYIRSGKAKENVPNLEITFLPEGSHFAQEQFPEQVNQLLLAFLTKHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZLP3</accession>
    <name>A0AA38ZLP3_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_013546</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZLP3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11072">
        <property type="entry name" value="CYP71-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000008">
        <property type="entry name" value="Cytochrome P450 71D8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47955:SF8">
        <property type="entry name" value="CYTOCHROME P450 71D11-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47955">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 71 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8 9" id="KW-0349">Heme</keyword>
    <keyword evidence="6 8 9" id="KW-0408">Iron</keyword>
    <keyword evidence="4 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="8">
        <location>
            <position position="442"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9691396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="505" mass="57442" checksum="386E860FF5488CE7" modified="2024-01-24" version="1">MDLLVSSILFAFLLFLYMLYKMGETSKASISTQKLPPGPWKLPLIGNMHQLVGSLPHQSLSRLAKKYGPLMSLQLGEVSTLTISSPEMAKKVLKIHDINFAQRSPLLASNVLSYDSTDIVYSPYGDYWRQLRKICVVELLTSKRVKSFQLVRKEEISNLITAVVSCSRLPINLTQNLSSYTFSVIARSAFGEKFEGQDAFISVTKEMVELYSGFCVADMYPSVKWLDLISGMRYKLDKVFQRTDRILQNIVDEHREKMKTEAGKLQGEEDLVDVLLKLQQHGDLEFPLTDNNIKGVILDIFAGGSETTSTSVDWAMSEMLRNPRVMEKAQAEVRRVFDGKGNVDETGLDELKFLKAVVKETLRLHPPFPLLIPRECREMCEIDGYEIPEKTRIIVNAWAIGRDSDYWVEAERFYPERFLDSSIDYKGTDFGYIPFGAGRRICPGIMFAMPGIELPLAHLLYHFDWKLPNGMKAEDLDMTEAFGLAVRRKQDLHLIPIPYNPSHAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZLP9</accession>
    <name>A0AA38ZLP9_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_013020</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZLP9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055283">
        <property type="entry name" value="TAXIMIN_1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33834">
        <property type="entry name" value="SIGNALING PEPTIDE TAXIMIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33834:SF14">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9690643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="78" mass="8469" checksum="CA490C580E831AF2" modified="2024-01-24" version="1">MCCGDDCECRPLGFLLGLPFAFLSLLLSIIGVIIWIVGLVLTCICPCCLCVTIIVELALGLIKAPIHVMKWFTSKIPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZLU4</accession>
    <name>A0AA38ZLU4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">N-acetyl-D-glucosamine kinase</fullName>
            <ecNumber evidence="2">2.7.1.59</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">GlcNAc kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_013397</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic-type N-acetylglucosamine kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.59" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZLU4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045127">
        <property type="term" value="F:N-acetylglucosamine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd24081">
        <property type="entry name" value="ASKHA_NBD_DdNAGK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002731">
        <property type="entry name" value="ATPase_BadF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052519">
        <property type="entry name" value="Euk-type_GlcNAc_Kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43190">
        <property type="entry name" value="N-ACETYL-D-GLUCOSAMINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43190:SF1">
        <property type="entry name" value="N-ACETYL-D-GLUCOSAMINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01869">
        <property type="entry name" value="BcrAD_BadFG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase BadF/BadG/BcrA/BcrD type" evidence="5">
        <location>
            <begin position="31"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00031123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9691205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="358" mass="37550" checksum="6604BA841AD8A7E6" modified="2024-01-24" version="1">MKNGYTNGDVWSFEGHHQALPPPDAPSHVVLGLDGGTTSTVCVCIPLQHLHLHPHSDPPPLLARAVAGCSNHNSVGEAAARETLEQVMAEALSKSGSNRSAVRAVCLGVSGVNHPTDQEMILSWLRPIFPSHVKLYVQNDSVAALASGTMGKLHGCVLIAGTGCISYGITEDGREARAAGAGPVLGDWGSGYGIAAQALTAIIRAQDGRGPQTMLTNSILNTLDLSSADELIGWTYADRSWARIAALVPEVVSCAEAGDQIANKILVDAVQELALSVKAVVQRLSLCGEDGNGSFPIVMVGGVLEANKRWDIGKEVISCIHKNFPGACPIRPKVEPAVGAALLAWNFLTKESKGNCYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZM37</accession>
    <name>A0AA38ZM37_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Superoxide dismutase</fullName>
            <ecNumber evidence="4 12">1.15.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_013718</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Destroys radicals which are normally produced within the cells and which are toxic to biological systems.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>2 superoxide + 2 H(+) = H2O2 + O2</text>
            <dbReference type="Rhea" id="RHEA:20696"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:18421"/>
            <dbReference type="EC" id="1.15.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the iron/manganese superoxide dismutase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.15.1.1" evidence="4 12"/>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZM37"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042644">
        <property type="term" value="C:chloroplast nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.990:FF:000002">
        <property type="entry name" value="Superoxide dismutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.55.40.20:FF:000007">
        <property type="entry name" value="Superoxide dismutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.990">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.55.40.20">
        <property type="entry name" value="Iron/manganese superoxide dismutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001189">
        <property type="entry name" value="Mn/Fe_SOD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019833">
        <property type="entry name" value="Mn/Fe_SOD_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019832">
        <property type="entry name" value="Mn/Fe_SOD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019831">
        <property type="entry name" value="Mn/Fe_SOD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036324">
        <property type="entry name" value="Mn/Fe_SOD_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036314">
        <property type="entry name" value="SOD_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42769">
        <property type="entry name" value="SUPEROXIDE DISMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42769:SF10">
        <property type="entry name" value="SUPEROXIDE DISMUTASE [FE] 3, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02777">
        <property type="entry name" value="Sod_Fe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00081">
        <property type="entry name" value="Sod_Fe_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000349">
        <property type="entry name" value="SODismutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01703">
        <property type="entry name" value="MNSODISMTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54719">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46609">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00088">
        <property type="entry name" value="SOD_MN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="7 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0934">Plastid</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Manganese/iron superoxide dismutase N-terminal" evidence="13">
        <location>
            <begin position="2"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="Manganese/iron superoxide dismutase C-terminal" evidence="14">
        <location>
            <begin position="81"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000349-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9691611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="198" mass="22801" checksum="1A68D61213D836AC" modified="2024-01-24" version="1">MSRRTLEMHWGEHHRGYVEGLNKQLEKDDILYGHTLDELVKVTYNNGNPLPEFNNAAQVWNHDFFWESMQPGGGDMPRLGLLEQIEKDFGSFTNFKEKFLEAALTLFGSGWVWLVLKRTERCLAVVKTSNAINPIVWDDIPIISLDLWEHAYYLDYKNDKGKYANVFMNHLVSWNAAAARMARAEAFVNLGEPKIPIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZPJ7</accession>
    <name>A0AA38ZPJ7_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_011850</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZPJ7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050317">
        <property type="entry name" value="Plant_Fungal_Acyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31642:SF266">
        <property type="entry name" value="HXXXD-TYPE ACYL-TRANSFERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31642">
        <property type="entry name" value="TRICHOTHECENE 3-O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9692928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="461" mass="50905" checksum="658600FD28F059BD" modified="2024-01-24" version="1">MGGGDFTVTESRKEVVGAVLPVQEYWLPLSNLDLLLPPVDVGVFFCYKKPHDLTFGTMISVLKEALAQALVSYYPFAGEVLSNSAGEPELLCNNRGVDFMEAYADVQLQNLNLYNPDESIESKLVPKKKHGVLSVQVTELKCGGVVVACTFDHRIADAYSANMFLVSWAEMAQSKPLSVLPSFRRSLLNARRPGSYDPSLDDMYVPMSALPPPKAPESGDDLLINRLYYVTAEQLSLLQSLATSKSGPCKRTKLESLSAFLWKMVAKSAVTEDANQKICRMGTVVDGRKRLSSGDEVKAAMMASYFGNVLSIPFGKKTINELKEKPLSWVADAIHEYLEGAVTKEHFLGLIDWVEAHRPEPALAKIYSSGSRDGPAFVVSSGQRFPASRVDFGWGMPALGSYHFPWGGEAGYVMPMPSPVRDGDWVVYMHLSVRQIEWIETEAAHIFRPLNSEYLNLSNSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZQ11</accession>
    <name>A0AA38ZQ11_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HXXXD-type acyl-transferase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_012066</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9693165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZQ11"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050317">
        <property type="entry name" value="Plant_Fungal_Acyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31642:SF266">
        <property type="entry name" value="HXXXD-TYPE ACYL-TRANSFERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31642">
        <property type="entry name" value="TRICHOTHECENE 3-O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9693165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="470" mass="51892" checksum="9B0BF56421EFBF0B" modified="2024-01-24" version="1">MGGGGKYFTVTVTRKELVAGVVPMQEHWLPLSNLDLLLPPIDVSVFFCYKKPHQNANGGDDFTFGSMVGVLRKALAQALVSYYVFAGEVVSNSVGEPELLCNNRGVDFMEAYADAKLHDLNLYNPDDSIEGKLVPKKQHGVLCVQVTELKCGGVVVGCTFDHRIADAYSANMFLVSWAEMALCKPLSVVPSFRRSLLNTRRPGSYDPSLDEMYVLISALPPPRAPQPGGADPFISRIYYVTAEQLSLLQTLASTSKGNFYKRTKLESFSAFLWKMVAASAVMENDNKKICKMGIVVDGRRRLSSGDEDKSAVMASYFGNVLSIPFGEKMIDELKEKPLSWVADAVHEYLEGAVTKEHFLGLIDWVEAHRPEPALAKIYCSGSRDGPAFVVSSGQRFPASKVDFGWGRPALGSYHFPWDGEAGYVMPMPSPARDGDWVVYMRLLKGQIELIETEAAHVFRPLTSEYLNLIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZQ82</accession>
    <name>A0AA38ZQ82_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transcription repressor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Ovate family protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_011451</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Transcriptional repressor that regulates multiple aspects of plant growth and development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZQ82"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038933">
        <property type="entry name" value="Ovate"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006458">
        <property type="entry name" value="Ovate_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01568">
        <property type="entry name" value="A_thal_3678"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33057:SF117">
        <property type="entry name" value="TRANSCRIPTION REPRESSOR OFP14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33057">
        <property type="entry name" value="TRANSCRIPTION REPRESSOR OFP7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04844">
        <property type="entry name" value="Ovate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51754">
        <property type="entry name" value="OVATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0678">Repressor</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="OVATE" evidence="8">
        <location>
            <begin position="186"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="87"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9692399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="259" mass="29491" checksum="271A56DC0611B5E3" modified="2024-01-24" version="1">MPRKLQKSLHDYLSKLKKPTPHIQLSANNALSASINRMLLGCKYPKTFIDVDRDQTNSRGSDHGRAATLSDIDRFLFENFRSLYQENDDDDIKKREEEEEEEKPGGVLFESPRIMDPPSNICRCHRFTTTPCSSSSFMEDATASLAASSEGIASSHTTSRPSTVDCSSSSSNDIKEILAPHDCIAVLTNSPNPYVDFRLSMQEMIEARLHSHEKVDWDFMEDLLFCYLNLNDKDSHKHILQAFVDLICVLRQKKPTKFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZQV2</accession>
    <name>A0AA38ZQV2_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_012014</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9693102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZQV2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9693102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="225" mass="26790" checksum="976E836802D03DB3" modified="2024-01-24" version="1">MVSGRVPKRRSYSSEYRGIGDRRRIKSLRVLENKRKKVSYPGHYRRRDTIRGSIGEYQIDRESNPCEYRRIQDRNDGIQASEESNPCEYRRIKERKHPIWATTEEEILSERVSENTRQTRNRIRASTGESKTKMMVSGRLSEKRSYSGEYRRIEMMIPRQLPEKRSYSGKYRRVTNRQGINPSEFCRIQDRKDGIRASTREEIIFGRVPGNRRHTKNRIRTSTGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZRL4</accession>
    <name>A0AA38ZRL4_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_009590</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9693709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZRL4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9693709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="97" mass="11316" checksum="E7CF3606DC777375" modified="2024-01-24" version="1">MELSIANHGSKNDLTVDLPKERHDGKRSDKTLKKPIQESMTINMAPVKISARDKKKKVKKNGPIQENERRRFTMKELEEKKYHFPDSDVPNMLEDLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZS07</accession>
    <name>A0AA38ZS07_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">L-2-hydroxyglutarate dehydrogenase, mitochondrial</fullName>
            <ecNumber evidence="7">1.1.99.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_009957</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>(S)-2-hydroxyglutarate + A = 2-oxoglutarate + AH2</text>
            <dbReference type="Rhea" id="RHEA:21252"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:16782"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="EC" id="1.1.99.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the L2HGDH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.99.2" evidence="7"/>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZS07"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047545">
        <property type="term" value="F:(S)-2-hydroxyglutarate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43104">
        <property type="entry name" value="L-2-HYDROXYGLUTARATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43104:SF4">
        <property type="entry name" value="L-2-HYDROXYGLUTARATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="9">
        <location>
            <begin position="37"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00036066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9694233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="424" mass="46811" checksum="20195FBC173A7CB3" modified="2024-01-24" version="1">MLRHTIRGLRNSRRWISSKNFTSRVGVERAVARESVDCVVIGAGVVGIAVARELALKGREVVVIESASTFGTGTSSRNSEVIHAGIYYPRNSLKAVFCVRGRELLYRYCSEHEVSHKQIGKLIVATRSSEVPKLNDLMIRGNENGVDGLRMLEGFEAMEMEPELQCVRALLSPASGIVDTHSLMLSLVGEAENHRTTFCYNTAVIGGHLEGDRICLHISESKDLENWDRRFPLHPEVILMAKLVVNSAGLSAPALAKRFDGLNSTVIPALHYARGCYFVLSKMKTPPFKHLIYPIPEDGGLGVHVTLDLDGQIKFGPDVEWINGVDDISSFLNKFDYSVCAKRVERFYPEIRKYYPNLKDDSLEPGYTGIRPKLSGAQQSAVDFIIQGEDIHGVPGLVNLFGIESPGLTSSMAIAEHIVARYLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZSQ5</accession>
    <name>A0AA38ZSQ5_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_010150</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZSQ5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9694517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="59" mass="6651" checksum="DAC032BB790D6EF4" modified="2024-01-24" version="1">MLSSFRLDQGELSSNFRLKQTREHSTPGSHCLDNVVSALLPRKSPRPCREAPVVVKFKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZTH8</accession>
    <name>A0AA38ZTH8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glycerol-3-phosphate acyltransferase RAM2/GPAT1-8 HAD-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_010410</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZTH8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056462">
        <property type="entry name" value="HAD_RAM2/GPAT1-8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23270">
        <property type="entry name" value="HAD_RAM2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycerol-3-phosphate acyltransferase RAM2/GPAT1-8 HAD-like" evidence="1">
        <location>
            <begin position="33"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF23270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9694921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="150" mass="16641" checksum="4333BF47D2FEADAA" modified="2024-01-24" version="1">MGAQRHFNPISKLAALRVEPIRQRLQTWIARFLCPEVLFPTSMLVAFEAGSLVRAILLLASVPLVEFGGPLPRPFREWSTHYPASAGLNWPCSLTGVQGWIFCVHPEDLGARPASSRGSPCIYEFRLYMSSNGTKYELFASSIDGDAMIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZUM3</accession>
    <name>A0AA38ZUM3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_010624</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZUM3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000111">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000307">
        <property type="entry name" value="pollen receptor-like kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000095">
        <property type="entry name" value="protein STRUBBELIG-RECEPTOR FAMILY 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046959">
        <property type="entry name" value="PRK1-6/SRF4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48007">
        <property type="entry name" value="LEUCINE-RICH REPEAT RECEPTOR-LIKE PROTEIN KINASE PXC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48007:SF4">
        <property type="entry name" value="LEUCINE-RICH REPEAT RECEPTOR-LIKE PROTEIN KINASE PXC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041257898" description="Protein kinase domain-containing protein" evidence="13">
        <location>
            <begin position="29"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="276"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="371"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="236"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="305"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="657"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9695225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="683" mass="74261" checksum="59752E9BD618D2F5" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZV89</accession>
    <name>A0AA38ZV89_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_010435</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZV89"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9694948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="150" mass="17050" checksum="AE1614950B5BF4FC" modified="2024-01-24" version="1">MVFTLTFYSPNLTSFSCDACQNAYWFACAGDDENSSSPNQDATHPVLLDLQMAHRLFKRPLTKKQFDNLKDDLQPSLQIRLKQLLESKQACEDVLEMADSAYVLMLLNSSNHQGEEARRAIMWFQEGVRLITDGQHLLKSAFPDVFTATD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZXA5</accession>
    <name>A0AA38ZXA5_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_008989</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZXA5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043240">
        <property type="term" value="C:Fanconi anaemia nuclear complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035428">
        <property type="entry name" value="FANCF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14449:SF2">
        <property type="entry name" value="FANCONI ANEMIA GROUP F PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14449">
        <property type="entry name" value="FANCONI ANEMIA GROUP F PROTEIN FANCF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11107">
        <property type="entry name" value="FANCF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9697026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="311" mass="35370" checksum="5ADA82CAF68C87FC" modified="2024-01-24" version="1">MGWSHPDVSLDDMVKLIKGFVDILILASGYQSSGHFAIWDPQNIKRAFQWGLFFENVIRHLSSSDDYQDSLKELDAVLLEIKSNPSSPQGLAQLSSVTLSRAKSYVLEHLIQTLTLRDAHLEAFLTASIGMDLDELSGTEQDCLSLYMNRLMLQNPSANMKTEKCTDDDFTTCTLQELLKRQFAVLCVSSTEKGLDILSKTIRHNNWTESESNSFKKLNQVTPLPSHDQLVDFITWSRWRSKNLSYLLDKRTVRLVSGASLIFSAPTVQWAQLFERLNISGETRVDLLFHSCRIELVNLEYTKKAIDDSWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZY85</accession>
    <name>A0AA38ZY85_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TOG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_009052</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZY85"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005881">
        <property type="term" value="C:cytoplasmic microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031110">
        <property type="term" value="P:regulation of microtubule polymerization or depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902903">
        <property type="term" value="P:regulation of supramolecular fiber organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000580">
        <property type="entry name" value="Protein peg1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024395">
        <property type="entry name" value="CLASP_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048491">
        <property type="entry name" value="XMAP215_CLASP_TOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21567">
        <property type="entry name" value="CLASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21567:SF9">
        <property type="entry name" value="CLIP-ASSOCIATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12348">
        <property type="entry name" value="CLASP_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21041">
        <property type="entry name" value="XMAP215_CLASP_TOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="TOG" evidence="7">
        <location>
            <begin position="3"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="5">
        <location>
            <begin position="83"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="5">
        <location>
            <begin position="160"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="TOG" evidence="7">
        <location>
            <begin position="282"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="domain" description="TOG" evidence="7">
        <location>
            <begin position="780"/>
            <end position="1030"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="506"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="602"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="722"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="521"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="723"/>
            <end position="733"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9697115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1224" mass="135822" checksum="1C4F212964088DF3" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZZU2</accession>
    <name>A0AA38ZZU2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_007430</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZZU2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010200">
        <property type="term" value="P:response to chitin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26374:SF379">
        <property type="entry name" value="ZINC FINGER PROTEIN ZAT12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26374">
        <property type="entry name" value="ZINC FINGER PROTEIN ZAT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="41"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="85"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9698346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="159" mass="17860" checksum="B00B015A70172AFC" modified="2024-01-24" version="1">MKRSRVDGVDEVEALAMANCLMLLSRVGKSESTNQLPGRVFECKTCSRKFPSFQALGGHRASHKKPRLMGDELQLPTSPAKPKTHECSICGLEFAIGQALGGHMRRHRSEIHNPTPVSVVKKTNDERVFSLDLDLNLTPWENDLKIQFRKLPHMVDCIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A0R3</accession>
    <name>A0AA39A0R3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_007077</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9697784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A0R3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22152">
        <property type="entry name" value="F-box_AtAFR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057499">
        <property type="entry name" value="Kelch_FKB95"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46344:SF3">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46344">
        <property type="entry name" value="OS02G0202900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25210">
        <property type="entry name" value="Kelch_FKB95"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="F-box" evidence="3">
        <location>
            <begin position="79"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="FKB95-like N-terminal Kelch" evidence="4">
        <location>
            <begin position="160"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9697784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="436" mass="49448" checksum="F568B2FC1F4FCF81" modified="2024-01-24" version="1">MDQRVEQSPNAQRGFRVQAPLVDSVSCYCKVDSGLKTVAGARKFVPGSKLCIQPDINPHAHKSKNSRRERTRFQPPLLPGLPDDLAIACLIRVPRVEHRKLRLVCKRWHRLLSGNFFYSLRKSLGMAEEWVYVIKRDRDGRISWHAFDPTYQLWQPLPPVPVEYSEALGFGCAVLSGCHLYLFGGKNPKGSMRRVIFYSARTNKWHKAPEMLRKRHFFGSCVINNCLYVAGGECEGIQRTLRSAEVYDPNRNRWNFISDMSTAMVPFIGVIYNGKWFLKGLGSHREVMSEAYIPETNTWTPISDGMVAGWRNPSISLNGQLYALDCRDGCKLRVYDRDTDSWNKFIDSKLHLGSSRALEAAALVPLNGKLCIIRNNMSISIVDVSSPDKHVESNPHLWENIAGKGHFRTLVTNLWSSIAGRNGLRSHIVHCQVLQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A141</accession>
    <name>A0AA39A141_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TLC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_007825</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A141"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006634">
        <property type="entry name" value="TLC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050846">
        <property type="entry name" value="TLCD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13439">
        <property type="entry name" value="CT120 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13439:SF0">
        <property type="entry name" value="TOPOISOMERASE I DAMAGE AFFECTED PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03798">
        <property type="entry name" value="TRAM_LAG1_CLN8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00724">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50922">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="109"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="189"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="229"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="TLC" evidence="7">
        <location>
            <begin position="57"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9698941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="267" mass="30240" checksum="FAD87BE4DC171635" modified="2024-01-24" version="1">MVGLPFHPAFMGLVSSSKEFHWMASVLFGMIFCKIVYELTGLLSNLCFKGYTKLSNTEKVEWNNRGFSTLHAIIVAVASLYLLLVSDLFDEDSHDESIINRASTLSDTILGISIGYFLSDLAMILWNFPALGGLEYVLHHGLSMFSIFLSLTSGQGQVYILMVLFSESTTPFVNLRWHLDVAGLKGSNLYICNGIALFFGWLVARILLFIFFFYHMIIHFDEVKKIFPLGFYSLLMVPPVLAMMNAFWFWKIAKGLIKTLSKARHSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A3J4</accession>
    <name>A0AA39A3J4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glucan endo-1,3-beta-D-glucosidase</fullName>
            <ecNumber evidence="3">3.2.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_008030</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;3)-beta-D-glucosidic linkages in (1-&gt;3)-beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.39"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the glycosyl hydrolase 17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.39" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9699224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A3J4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000010">
        <property type="entry name" value="glucan endo-1,3-beta-glucosidase, basic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000490">
        <property type="entry name" value="Glyco_hydro_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044965">
        <property type="entry name" value="Glyco_hydro_17_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32227">
        <property type="entry name" value="GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00332">
        <property type="entry name" value="Glyco_hydro_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00587">
        <property type="entry name" value="GLYCOSYL_HYDROL_F17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041463894" description="glucan endo-1,3-beta-D-glucosidase" evidence="8">
        <location>
            <begin position="30"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9699224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="345" mass="37288" checksum="2F2B1C34DAE641A7" modified="2024-01-24" version="1">MALTRNRPFVVVLLLGFVIMSSITIGAQSIGVCYGTNGNNLPSASQVINLYKSNGIGSMRIYDPNSDTLQALRGSGIELILDVPNTSLQSLASDASAASTWVQNNVVNYASDVKFRYIAVGNEVSPTSDSTSQYAQYVLPAMKNVQSAITSAGLQDQIKVSTATYSAVLDNTYPPSHGSFKNDVSSFINPIISFLAENGSPLLANIYPYFSYTDDTQNIRLDYALFTSSGVVVQDGSYQYQNLFDALLDALYAALEKAGGSNLQIVVSESGWPSEGGTAATVDNARTYYSNLINHVKGGTPRKGQAIETYLFAMFDENQKTGLETEKHFGLFTPSQESKYQISFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A6D2</accession>
    <name>A0AA39A6D2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ankyrin repeat protein SKIP35</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_006969</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A6D2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044956">
        <property type="entry name" value="SKIP35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36024">
        <property type="entry name" value="ANKYRIN REPEAT PROTEIN SKIP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36024:SF1">
        <property type="entry name" value="OS11G0246900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="44"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9701820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="626" mass="67926" checksum="EE27E8E7EDF286F5" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A6P7</accession>
    <name>A0AA39A6P7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_006916</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A6P7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080043">
        <property type="term" value="F:quercetin 3-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080044">
        <property type="term" value="F:quercetin 7-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000019">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000429">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058980">
        <property type="entry name" value="Glyco_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926">
        <property type="entry name" value="GLUCOSYL/GLUCURONOSYL TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926:SF1560">
        <property type="entry name" value="UDP-GLYCOSYLTRANSFERASE 74E1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26168">
        <property type="entry name" value="Glyco_transf_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase N-terminal" evidence="5">
        <location>
            <begin position="10"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF26168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9701749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="457" mass="51652" checksum="74CD278EBD5E88C2" modified="2024-01-24" version="1">MSREKRVGEIHIMVFPFPLQGHINPMLQFSKRLASKGLKVTLLMAASSINKSVQDQATSSINIELIANYESDPNKKQEDIKAYLEKFKVAASQSLSEVIEKHNRSDHPAKILVYDSIMPWAQDLAERLDLEGARFFTQSCAVSTIYYHANQGAFKNPLEGSTVSMPSMPVLGINDMPSFIKEMGSYPASLALLLNQFLNLQKVKWVFFNTFNKLEDEVVKWLANHQSVKTIGPTIPSMYLDKRLEDDKDYGLSLFKPNTDTCITWLDTKDINSVVYVSFGSMASLGEEQMEELAWGLKRSNGYFLWVVRESEEEKLPTNFVEETSEKGLFVSWCHQVEVLAHKAVGCFMTHCGWNSTLEALSQGVPMIAMPCWADQPTNAKFVEDVWEVGVRVKVDEKGIAKREEIEECIREVMEGERGNEMKRNGEKWKELGKEAVNEGGSSDNNIEEFVAQLVCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A7J9</accession>
    <name>A0AA39A7J9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serine/threonine protein phosphatase 2A regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_004249</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the phosphatase 2A regulatory subunit B56 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A7J9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000041">
        <property type="entry name" value="Serine/threonine protein phosphatase 2A regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002554">
        <property type="entry name" value="PP2A_B56"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10257">
        <property type="entry name" value="SERINE/THREONINE PROTEIN PHOSPHATASE 2A PP2A REGULATORY SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10257:SF59">
        <property type="entry name" value="SERINE_THREONINE PROTEIN PHOSPHATASE 2A 57 KDA REGULATORY SUBUNIT B' KAPPA ISOFORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01603">
        <property type="entry name" value="B56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028043">
        <property type="entry name" value="PP2A_B56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="26"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR028043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9702423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="492" mass="56152" checksum="0E2A63E02AC8BB06" modified="2024-01-24" version="1">MLKQILSRLPRKALKTESGDLVRTDTGTPRSSSGSPGSRPTSAKKTSSVFPTNAVAGVEPSAAFNDVPIAEKLNLFVSKLNLCCTVFDFNDPSKNSIEKDVKRQTLIELVDFMVSGPPRFTEPVIAAMCKVCATNLFRVFPPNYRSNYLGGENDDDEPTFDPAWSHLQLVYDLLLKFVTSSCLDAKVAKKYIDHSFILRLLDLFDTEDPRERECLKTVLHRIYGKFMVHRPFIRKSMGNIFYRFVFETERNNGIAEMLEIFGSVISGFALPLKEEHKIFLWRALIPLHKPKSVGVYFQQLSYCVTQFIEKEPKLASSVIKGLLRYWPITNSQKEVMFLGELEEILEAINMVEFQKIMVPLFWRIGCCINSFHFQVAERALFLWNNDQIVNLIAHNRQVLIPVIFPALERNAQSHWNQAVLNLTLNVRKMLSEMDDELFLACHARFKEEESRLSLEAQKRKETWEQLENEASLQPVSGNTAVLVTPLATSITC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AC31</accession>
    <name>A0AA39AC31_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein RER1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_003013</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in the retrieval of endoplasmic reticulum membrane proteins from the early Golgi compartment.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the RER1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AC31"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006621">
        <property type="term" value="P:protein retention in ER lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004932">
        <property type="entry name" value="Rer1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10743">
        <property type="entry name" value="PROTEIN RER1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10743:SF17">
        <property type="entry name" value="PROTEIN RER1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03248">
        <property type="entry name" value="Rer1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016013">
        <property type="entry name" value="AtER_Rer1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="140"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR016013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9704761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="194" mass="22112" checksum="F946FABEBFE4036A" modified="2024-01-24" version="1">MEGTGGGGPSAVSPVIQWKTDASRLFQYYLDKTTPHPVYRWVGTLVVAAIYVLRVFYVQGFYVVSYGLGIYLLNLLIGFLSPLVDPELETSNEALLPTKGSDEFKPFIRRLPEFKFWYSITKAFCVAFVLTFFSAFDVPVFWPILLCYWIVLFILTMKRQIVHMIKYKYVPFSIGKQKYGGKKSFASSSGSNMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AD47</accession>
    <name>A0AA39AD47_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ResB-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_003076</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AD47"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017004">
        <property type="term" value="P:cytochrome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01392">
        <property type="entry name" value="CytC_Ccs1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023494">
        <property type="entry name" value="Cyt_c_bgen_Ccs1/CcsB/ResB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007816">
        <property type="entry name" value="ResB-like_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31566">
        <property type="entry name" value="CYTOCHROME C BIOGENESIS PROTEIN CCS1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31566:SF0">
        <property type="entry name" value="CYTOCHROME C BIOGENESIS PROTEIN CCS1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05140">
        <property type="entry name" value="ResB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0201">Cytochrome c-type biogenesis</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="115"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="260"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="ResB-like" evidence="8">
        <location>
            <begin position="121"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="ResB-like" evidence="8">
        <location>
            <begin position="450"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="75"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF05140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9704855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="557" mass="60235" checksum="DCDB5E4F7EF63920" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ADB7</accession>
    <name>A0AA39ADB7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PB1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_003500</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ADB7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06410">
        <property type="entry name" value="PB1_UP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000058">
        <property type="entry name" value="Octicosapeptide/phox/Bem1p domain kinase superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053198">
        <property type="entry name" value="Gynoecium_Dev_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000270">
        <property type="entry name" value="PB1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31066">
        <property type="entry name" value="OS05G0427100 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31066:SF100">
        <property type="entry name" value="PB1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00564">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00666">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PB1" evidence="2">
        <location>
            <begin position="57"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="447"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9705486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="467" mass="50231" checksum="7C9240901FC62A1D" modified="2024-01-24" version="1">MENYTYTSYADSGESSPRSRELEFENSGAASWEDQPLHQPSKVKFMCSYGGKIQPRAHDNQLSYTGGETKILSVDRSIKFSSLINKLCVLCEADVCFKYQLPGEDLDALISVTNDDDLEHMMHEYDRLCRPSAKPARLRLFLFPATPPSVGSGDMKTERERFFEALNSGPIQSVDATSPPATAPPSNVDFLFGLDKGVSSPAPVAKLPVPVPEPPVPAEMPSLGNPGGDGRVFAADLGVNQAEIQRQIQDLQRLQISAQEQGAYQRKSDDNMYGGYAGDYYVQKMPEKAPPPSTIPATVPATYWQEKRAPGGGFPGGMQATPGLPEQPVYMIPAPASVYHTPVARPVTGQSGQGYYAMRMGPDVYREQTPVYSVMQQPPVPATMPAPAPKLPNYSEGLGMVRPSGVGVADTGYTQVAYDSQGRQVYYTAPGGVVAPYQAMAAVSTTSGPLNQDSGKVTAAKVSQASV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AE41</accession>
    <name>A0AA39AE41_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_003424</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AE41"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33700">
        <property type="entry name" value="MYB-LIKE PROTEIN X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33700:SF4">
        <property type="entry name" value="MYB-LIKE PROTEIN X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="170"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="195"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="232"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="253"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="284"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="309"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="334"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="355"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="380"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="401"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="429"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="451"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="478"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9705372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="507" mass="55623" checksum="A2773BE602E61688" modified="2024-01-24" version="1">MFKSPSRNQRSKGFKVKHILQICLLLAVCFWLIYQVKHSHDKKKEFDANDAKISSNTQGGNELLKFGRKDLHPRVDGEAKNEKHGEEETEEEDEAGGVEEEESKHDEEQEEEESKHDEEQEEEEGKADEREDEGRGGGDDEIDENDQERAEGEEEVLDEEKEREEEGDERESGEKESEEKDDLSENEKETDDQDHDGASRNAHEAREEHYKADDASSAVAHDAQTNGGEADKMEDFDENTLEQETKPNNNEVSIEHKNSKAEEVELAENEASSNVTDSQEKGTEINSPESENSSIQNPPITTESNVQPEVSNKNSSEVSNKDSSEVSNNDSSELSTKPSDSDTENGTETVLDPIQAQNATGEGINTEQGADMQHVVLKQTIDSTIVTDKNQSGSEPENAGATTGESSNSISSNATSEAEHNPELMISKENTDATQNEKPDTSNGSGETDESSASSSTNGSGDSIQQDPIDVSDSSIPQEEKESPPTDLDKTPDIRTNASDKEGAATE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AHH6</accession>
    <name>A0AA39AHH6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">SBP-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_000012</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9707736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AHH6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1100.10:FF:000001">
        <property type="entry name" value="Squamosa promoter-binding-like protein 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1100.10">
        <property type="entry name" value="Transcription factor, SBP-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044817">
        <property type="entry name" value="SBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004333">
        <property type="entry name" value="SBP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036893">
        <property type="entry name" value="SBP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31251:SF102">
        <property type="entry name" value="SBP-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31251">
        <property type="entry name" value="SQUAMOSA PROMOTER-BINDING-LIKE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03110">
        <property type="entry name" value="SBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103612">
        <property type="entry name" value="SBT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51141">
        <property type="entry name" value="ZF_SBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041326431" description="SBP-type domain-containing protein" evidence="11">
        <location>
            <begin position="21"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="domain" description="SBP-type" evidence="12">
        <location>
            <begin position="216"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="283"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="283"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9707736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="563" mass="62721" checksum="373284EB02668FD7" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AIP5</accession>
    <name>A0AA39AIP5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ACT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_002077</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AIP5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008864">
        <property type="term" value="F:formyltetrahydrofolate deformylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04875">
        <property type="entry name" value="ACT_F4HF-DF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.170">
        <property type="entry name" value="Formyl transferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002376">
        <property type="entry name" value="Formyl_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036477">
        <property type="entry name" value="Formyl_transf_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004810">
        <property type="entry name" value="PurU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044074">
        <property type="entry name" value="PurU_ACT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42706">
        <property type="entry name" value="FORMYLTETRAHYDROFOLATE DEFORMYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42706:SF1">
        <property type="entry name" value="FORMYLTETRAHYDROFOLATE DEFORMYLASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00551">
        <property type="entry name" value="Formyl_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036480">
        <property type="entry name" value="FormyFH4_hydr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01575">
        <property type="entry name" value="FFH4HYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53328">
        <property type="entry name" value="Formyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACT" evidence="3">
        <location>
            <begin position="47"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9706934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="258" mass="29197" checksum="2678C9DB5BF62B64" modified="2024-01-24" version="1">MSLRRRLSTSLPQLLGFTNRSFKSLKFPQQSLHCFNSSSESSLAHGIHVFQCPDGVGIVAKLSDCIASRGGNILGADIFVPENKKVFYSRSEFIFDPAKWPRAQMDEDFLKLSNMFNAMKSVVRVPNLDSKYKISVLASKQDHCLVDLLHGWQDGRLPVDITCVISNHDRGPNTHVFRFLERHGIPYHYLHTTKEHKREGEILDLVQDTDFLVLARYMQILSGNFLKSYGKDVINIHHGLLPSFKGGNPSKQIMLTGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AJB6</accession>
    <name>A0AA39AJB6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase</fullName>
            <ecNumber evidence="3">3.2.2.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_002494</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>NAD(+) + H2O = ADP-D-ribose + nicotinamide + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16301"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57967"/>
            <dbReference type="EC" id="3.2.2.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:16302"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the disease resistance TIR-NB-LRR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.6" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AJB6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061809">
        <property type="term" value="F:NAD+ nucleosidase activity, cyclic ADP-ribose generating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050832">
        <property type="term" value="P:defense response to fungus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043068">
        <property type="term" value="P:positive regulation of programmed cell death"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10140:FF:000007">
        <property type="entry name" value="Disease resistance protein (TIR-NBS-LRR class)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045344">
        <property type="entry name" value="C-JID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011713">
        <property type="entry name" value="Leu-rich_rpt_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058192">
        <property type="entry name" value="WHD_ROQ1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017:SF479">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN (TIR-NBS-LRR CLASS) FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20160">
        <property type="entry name" value="C-JID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07725">
        <property type="entry name" value="LRR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23282">
        <property type="entry name" value="WHD_ROQ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="TIR" evidence="13">
        <location>
            <begin position="11"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1090"/>
            <end position="1149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00061488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9707488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1149" mass="130462" checksum="03D49FEC5B598315" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AJH8</accession>
    <name>A0AA39AJH8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Alpha-galactosidase</fullName>
            <ecNumber evidence="3 7">3.2.1.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Melibiase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_000672</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.</text>
            <dbReference type="EC" id="3.2.1.22"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the glycosyl hydrolase 27 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.22" evidence="3 7"/>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AJH8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004557">
        <property type="term" value="F:alpha-galactosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14792">
        <property type="entry name" value="GH27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002241">
        <property type="entry name" value="Glyco_hydro_27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041233">
        <property type="entry name" value="Melibiase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11452:SF42">
        <property type="entry name" value="ALPHA-GALACTOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11452">
        <property type="entry name" value="ALPHA-GALACTOSIDASE/ALPHA-N-ACETYLGALACTOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16499">
        <property type="entry name" value="Melibiase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17801">
        <property type="entry name" value="Melibiase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00740">
        <property type="entry name" value="GLHYDRLASE27"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="Ricin B-like lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041324144" description="Alpha-galactosidase" evidence="8">
        <location>
            <begin position="24"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha galactosidase C-terminal" evidence="9">
        <location>
            <begin position="560"/>
            <end position="641"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF17801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9708771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="647" mass="72145" checksum="93ECBAD495B800AB" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AJP0</accession>
    <name>A0AA39AJP0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_000456</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the WUS homeobox family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AJP0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099402">
        <property type="term" value="P:plant organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000146">
        <property type="entry name" value="WUSCHEL-related homeobox 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044555">
        <property type="entry name" value="WUSCHEL-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45940:SF13">
        <property type="entry name" value="WUSCHEL-RELATED HOMEOBOX 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45940">
        <property type="entry name" value="WUSCHEL-RELATED HOMEOBOX 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 9 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="90"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="92"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="143"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="148"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9708424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="337" mass="38308" checksum="0DBEB8CDF4E4A041" modified="2024-01-24" version="1">MWTMDSSDSSDLTMSDSFHGRKLRPLIPRPNASVLPKITVNSTVAISPCISHLHGTDLFALNHHLGGTSDQQSKTDFNTQPFGSSRWNPTPEQLQALEELYRRGTRTPTAEQIQQIAAQLRLFGKIEGKNVFYWFQNHKARERQKRRRELESDSEEQQRDNGSLERKESVLRRTCIEVEQTKNWVVPSSNCSTLSEESVSIQRAAVAESRTDGWLQLEERELQQTRSTVERQATWQLMGLTSSPPSQLIDTITTTTVAEALNTIYPKQKPLSPQSLRPNTYLEHETRESQTLQLFPVQGAQSFNAANEIISVAEMENGLPITTIDTNFTPNQFFEFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AKL3</accession>
    <name>A0AA39AKL3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_001007</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Potential calcium sensor.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AKL3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000302">
        <property type="entry name" value="Probable calcium-binding protein CML46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039647">
        <property type="entry name" value="EF_hand_pair_protein_CML-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10891">
        <property type="entry name" value="EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="108"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="146"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9709322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="180" mass="20741" checksum="C8F1EEF25A801C3A" modified="2024-01-24" version="1">MEKTPTNTTLAGFLLAFKINRCFWLFQRFIQPLVNFASTRWKVWTKAKSPDPDPVLGPQHDTGHNRKLKGAELKMVMDRLGVTYHSDGEKLEEGLVADELPQLFEEEPSLEEVKEVFDVFDENKDGFIDATELQRVLCSLGLKEGSQVEDCRRMIKAFDEDDDGQIDFKEFVKFLEKSFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AKW0</accession>
    <name>A0AA39AKW0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA-3-methyladenine glycosylase I</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_001069</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AKW0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008725">
        <property type="term" value="F:DNA-3-methyladenine glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005019">
        <property type="entry name" value="Adenine_glyco"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31116:SF43">
        <property type="entry name" value="DNA GLYCOSYLASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31116">
        <property type="entry name" value="OS04G0501200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03352">
        <property type="entry name" value="Adenine_glyco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="40"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="51"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="64"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="374"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="378"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR605019-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9709411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="398" mass="44053" checksum="5C373340A77C6B0F" modified="2024-01-24" version="1">MCSFKSKLHQGIDITPSKAQINGRPALQPTCNRIPSLERHHSFKKISPKSPTSPLPASLPPPTTIINTTKTKPSLTPPASPNSKSPRQPALKRGNDPNGLNSSLEKVLTPRGTTKSSSSPKKTKKCSAGLAPSSDTSSFNYSSSLIVEAPGSIAAARREQMAIMQVQRKMRIAHYGRTKSAKYEEKIGPVDPLVITTREEKRCSFITPNSDPSYVEYHDEEWGVPVHDDKRLFELLVMTGAQVGSDWTTVLKKRQEYRDAFSGYDAEIVAKFSEKKITSISAYYGIDLSQVRGVVDNSSRILEIRREFGSFHKYIWGFVNHKPITTQYKSCHKIPVKTSKSESISKDMVRRGFRLVGPTVIHSFMQAAGLTNDHLISCPRHLQCIALSSHQPAVAPAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AQJ6</accession>
    <name>A0AA39AQJ6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_001331</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Potential calcium sensor that binds calcium in vitro.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AQJ6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000237">
        <property type="entry name" value="Calcium-binding protein CML38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000003">
        <property type="entry name" value="Calmodulin A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039647">
        <property type="entry name" value="EF_hand_pair_protein_CML-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10891">
        <property type="entry name" value="EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="2"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="38"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="74"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="110"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00057710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9709813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="140" mass="16039" checksum="894339C061A645FF" modified="2024-01-24" version="1">MISCNVYERIFKRFDEDGDGKLSPSELRRCLGTIGKELLMEEAQELVESMDSDGDGLLGMEEFVGWMEREGEERKMEDLREAFRMYEMEGSGCITAKSLKRMLSRLGESRSVEECCVMIGQFDINGDGVLSFDEFKLMML</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D4R9</accession>
    <name>A0AA39D4R9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_025545</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D4R9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="125"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="155"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="179"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="213"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="242"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="341"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="370"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="398"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="422"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="51"/>
            <end position="510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9671948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="529" mass="57387" checksum="5AAD91EE1F6BAA9E" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D503</accession>
    <name>A0AA39D503_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">1,4-alpha-glucan branching enzyme</fullName>
            <ecNumber evidence="5">2.4.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_025713</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Transfers a segment of a (1-&gt;4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.</text>
            <dbReference type="EC" id="2.4.1.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Glycan biosynthesis; starch biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Amyloplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.18" evidence="5"/>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D503"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009501">
        <property type="term" value="C:amyloplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003844">
        <property type="term" value="F:1,4-alpha-glucan branching enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043169">
        <property type="term" value="F:cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001322">
        <property type="entry name" value="1,4-alpha-glucan-branching enzyme 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001359">
        <property type="entry name" value="1,4-alpha-glucan-branching enzyme 3, chloroplastic/amyloplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1180:FF:000045">
        <property type="entry name" value="1,4-alpha-glucan-branching enzyme 3, chloroplastic/amyloplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000093">
        <property type="entry name" value="1,4-alpha-glucan-branching enzyme 3, chloroplastic/amyloplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006048">
        <property type="entry name" value="A-amylase/branching_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037439">
        <property type="entry name" value="Branching_enzy"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="GH13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004193">
        <property type="entry name" value="Glyco_hydro_13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43651">
        <property type="entry name" value="1,4-ALPHA-GLUCAN-BRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43651:SF4">
        <property type="entry name" value="1,4-ALPHA-GLUCAN-BRANCHING ENZYME 3, CHLOROPLASTIC_AMYLOPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02806">
        <property type="entry name" value="Alpha-amylase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02922">
        <property type="entry name" value="CBM_48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000463">
        <property type="entry name" value="GlgB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0035">Amyloplast</keyword>
    <keyword evidence="7" id="KW-0934">Plastid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 13 catalytic" evidence="9">
        <location>
            <begin position="413"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="15"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="15"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="27"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9672188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="896" mass="103506" checksum="2727A196DFAD6172" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D6F3</accession>
    <name>A0AA39D6F3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_025469</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D6F3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF78">
        <property type="entry name" value="OS12G0587133 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9671770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="621" mass="71815" checksum="718F6516D2DC5BE7" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D6X9</accession>
    <name>A0AA39D6X9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transglycosylase SLT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_026270</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D6X9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00254">
        <property type="entry name" value="LT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.530.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008258">
        <property type="entry name" value="Transglycosylase_SLT_dom_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37179">
        <property type="entry name" value="TRANSGLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37179:SF1">
        <property type="entry name" value="TRANSGLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01464">
        <property type="entry name" value="SLT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="Lysozyme-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transglycosylase SLT" evidence="1">
        <location>
            <begin position="72"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="Transglycosylase SLT" evidence="1">
        <location>
            <begin position="310"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9672939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="414" mass="47601" checksum="76B9DF4714C0F4B8" modified="2024-01-24" version="1">MALSYQYWDDCVDPRDLEALWLDPDVSTEWLDVGETRGRKVHLSRDPDGEPYLTQIEMKAVAAIVVSRHFNSQIDLEMICAISEIESDRQPLAERYDKKAKDTTLGIMQLLPKTADWLVRELGYQTYEVEGNPILLFRPFINVYLGAAYLKWLSNYDGKERSEEFVVRAYKGGPKKAAHKSTLEYWKRYLSVKESLPFRKSLYDGPCLNDASGSGAPVSVIKGVNHTFWDSRASPEDMDEMWNHPDVLKEWSKCGEIRGKVLFSHDKEKRPYLSRVEVKAVAEIILSKHFSTRQVKPTILCALAEIVSMRFVNGVGARTGIMGIDYPTAMWLYRDLGYKAYRVEAVDDLTKPFVSMYFGAAYLAWLSEYEGRQRTPQFIVQAYLAGPKNVNLQETGHHWLNFEKAITYYEPKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D8R9</accession>
    <name>A0AA39D8R9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_024853</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9676058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D8R9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04051">
        <property type="entry name" value="C2_SRC2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044750">
        <property type="entry name" value="C2_SRC2/BAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32246:SF22">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32246">
        <property type="entry name" value="INGRESSION PROTEIN FIC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="167"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="2">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9676058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="194" mass="21654" checksum="5216A384F169F95C" modified="2024-01-24" version="1">MEWRMFEITLISARNLEVRETQKTNVYAKISIAGHPNTEKRTPVDKKGGGNPAWNFTTTCMIGKQAVEHDVVKLEIKLYRSRALGDKYIGKVCVSFKELFNLERTLWGRLRTHFSSPKGAGVGKTVKYSVKKNGGSDSSGVLKFSYRFGKVEIVNQPSRQGITSTRVAELVVLLIRVVIAAALSTHIPFAWVVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D9S3</accession>
    <name>A0AA39D9S3_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_024757</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D9S3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="103"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9675939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="204" mass="23216" checksum="1F2D4D84BE515429" modified="2024-01-24" version="1">MLDFFQFTKWVSVNLEGDPLVFVMARLLFGTPESVISCIQPMQGYTAEQTTEVVVAGVRNLKRQRALLFKQLGVTEMMRTFITQKRGAIKNSAPTTANGAEALRRAEEEREMAQVEANHLREEVKATEAKDRDADQEIAHLRKELEESRAGFAVQKKELENKYHRQVDDIFFVGYRCCIKKNDITQDTPSYLSDDEGEAVCSFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D9X0</accession>
    <name>A0AA39D9X0_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_025079</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9676376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D9X0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9676376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="53" mass="5780" checksum="E6A8D8ECE47BF3D0" modified="2024-01-24" version="1">MNCGTQRSDKVEHNYESDKVENSQNSCYNSGETLKDEGISMATTSTRNGSSTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DAW2</accession>
    <name>A0AA39DAW2_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_021972</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9676720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DAW2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005540">
        <property type="entry name" value="KNOX1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005541">
        <property type="entry name" value="KNOX2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48452">
        <property type="entry name" value="FUSED COMPOUND LEAF 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48452:SF1">
        <property type="entry name" value="FUSED COMPOUND LEAF 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03790">
        <property type="entry name" value="KNOX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03791">
        <property type="entry name" value="KNOX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01255">
        <property type="entry name" value="KNOX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01256">
        <property type="entry name" value="KNOX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KNOX1" evidence="4">
        <location>
            <begin position="29"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="KNOX2" evidence="5">
        <location>
            <begin position="78"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="55"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="110"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="119"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9676720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="150" mass="16751" checksum="F3B0167A0FBFF47D" modified="2024-01-24" version="1">MQSDGSSDENGERVCGGGGRLEEEEEERKMLKRRISCHPLYGFLVEAHLDCLKVGLGSNNGKPGRSHKSDEKTRHNQPSLSMYSQSELDHFMEAYCTTLTKLKEAMEEPQQETLAFIDSMQSQLEELSGSQHEPPQPPTISSGKLKLTLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DAY6</accession>
    <name>A0AA39DAY6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Cytochrome P450 89A2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_020492</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DAY6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016709">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11075">
        <property type="entry name" value="CYP77_89"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000012">
        <property type="entry name" value="Cytochrome P450 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051103">
        <property type="entry name" value="Plant_metabolite_P450s"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24298:SF800">
        <property type="entry name" value="CYTOCHROME P450 89A2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24298">
        <property type="entry name" value="FLAVONOID 3'-MONOOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12 13" id="KW-0349">Heme</keyword>
    <keyword evidence="9 12 13" id="KW-0408">Iron</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 13" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="8 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="245"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="245"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="259"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="12">
        <location>
            <position position="438"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9678338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="494" mass="56194" checksum="7C11BDA142A20389" modified="2024-01-24" version="1">MDFDQQKMEIWVFFFIVSLCIASLLKSLHDFFFPKLNLPPGPAAFPLIGSLLWLGTYSADLEPILRNLHAKYGAILTLRIGSHPAIFISSNSLAHQALVQNGAVFSDRPAAGPADRVMSSNQRSINSCPNGPTWRLLRRNLTAEILHPSRVRSYSHARKWVLEILVSRLRGQSDGFVPVRKMDQFQYAMFCLLRRLLLGFLRFNRLDLLPRMGKVLFRKRWDELLNLRKDQEEILLPHIRARQRLKQETQSKREEDSSDPGSSSSSSSKDYVLSYIVTLCSEFLSAGTDTTSTALQWIMANLVKSPHIQARLFEEISGVVGEGQEEVKEEDLQKMPSLKAVVLEGLRRHPPGHFVLPHSVTQDVSFEGYDIPKNATVNFIVSEMNWNPKIWENPMEFKPERFLDNNGDGVHGDEGEAFDITGSREIKMMPFGAGRRICPGYGLAMLHLEYFVANLVWNFDWKAVEGDEVDLSEKLEFTVVMKNPLQAHLSPRLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DFX4</accession>
    <name>A0AA39DFX4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ALOG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_018406</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the plant homeotic and developmental regulators ALOG protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DFX4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009299">
        <property type="term" value="P:mRNA transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009416">
        <property type="term" value="P:response to light stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040222">
        <property type="entry name" value="ALOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006936">
        <property type="entry name" value="ALOG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31165">
        <property type="entry name" value="PROTEIN G1-LIKE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31165:SF70">
        <property type="entry name" value="PROTEIN LIGHT-DEPENDENT SHORT HYPOCOTYLS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04852">
        <property type="entry name" value="ALOG_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51697">
        <property type="entry name" value="ALOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="ALOG" evidence="9">
        <location>
            <begin position="38"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="157"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="9"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="172"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9682479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="181" mass="20174" checksum="27ACB4EB88D488E6" modified="2024-01-24" version="1">MDLVSQSGNPNSVSTIINPNNPNSSSTISSPAVYAPSRYENQKRRDWNTFGQYLRNHRPPLSLSLCNGAHVLEFLRYLDQFGKTKVHNQTCPFFGLKNPPAPCPCPLRQAWGSLDALIGRLRAAYEEHGGKPEANPFGARAVRLYLREVRDFQAKARGVSYEKKRKRSKPKLAPPPAGASQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DH16</accession>
    <name>A0AA39DH16_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_017994</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9681892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DH16"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9681892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="156" mass="18300" checksum="2B1C1EA994E496E8" modified="2024-01-24" version="1">MEGGSSCFSLHFGQIVPQLHLQNLHQQDHHQQQTIDYSRLTSFLEYDDSFLSVWNWKSIDDDDVNSSSHREESRPRYMEFQPGSLFNHDVFDLKLEYMEFEQLLEKASTSTKINDCEEVSSFTEINNYKEILSSEEIGDYEETSSSTETNDFKDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DH61</accession>
    <name>A0AA39DH61_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_016353</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9683814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DH61"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14798">
        <property type="entry name" value="RX-CC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.430:FF:000003">
        <property type="entry name" value="Probable disease resistance protein At5g66910"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038005">
        <property type="entry name" value="RX-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF1185">
        <property type="entry name" value="DISEASE RESISTANCE RPP8-LIKE PROTEIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="5">
        <location>
            <begin position="5"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="4">
        <location>
            <begin position="159"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="6">
        <location>
            <begin position="429"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="7">
        <location>
            <begin position="578"/>
            <end position="890"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9683814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="986" mass="112886" checksum="0DDCF5B6505EC466" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DIW0</accession>
    <name>A0AA39DIW0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NLE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_017604</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the NLE1/RSA4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DIW0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000092">
        <property type="entry name" value="notchless protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012972">
        <property type="entry name" value="NLE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001632">
        <property type="entry name" value="WD40_G-protein_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848:SF0">
        <property type="entry name" value="NOTCHLESS PROTEIN HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08154">
        <property type="entry name" value="NLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00319">
        <property type="entry name" value="GPROTEINB"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 6" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="NLE" evidence="7">
        <location>
            <begin position="15"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="108"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="150"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="193"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="240"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="360"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="402"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="444"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00061016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9685629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="477" mass="53248" checksum="352A76EE7BACB7FC" modified="2024-01-24" version="1">MEMEIEEQGETGKNVMCQLTDPEGNHLGPPMYLPQNAAPKELQQIVNKLLNNEEKLPYAFYISDQELVVQLGTYLQKNKVSVEKVLPIVYQPQAVFRIRPINRCSSTIAGHTEAVLSVAFSPDGRQLASGSGDTTVRLWDLNTQTPMYTCTGHKNWVLCIAWSPDGKHLVSGSKAGELQCWDPQTGKPSGNPLIGHKKWITGISWEPVHLRAPCRRFVSSSKDGDARIWDVSLRKCVICLNGHTLAVTCVKWGGDGVIYTGSQDCTIKVWETSQGKLIRELKGHGHWVNSLALSTEYVLRTGAFDHTGKQYTSPEEMKEVALERYKKMKGNAPERLVSGSDDFTMFLWEPADSKHPKTRMTGHQQLVNHVYFSPDGQWVASASFDKSVKLWNGTTGKFVAAFRGHVGPVYQISWSADSRLLLSGSKDSTLKVWDIRTHKLKQDLPGHEDEVFAVDWSPDGEKVASGGRDRVLKLWMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DJL4</accession>
    <name>A0AA39DJL4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycosyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_015940</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="5"/>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DJL4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035251">
        <property type="term" value="F:UDP-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000056">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000080">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050481">
        <property type="entry name" value="UDP-glycosyltransf_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48048">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48048:SF45">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9687263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="476" mass="53225" checksum="6911927D12A20586" modified="2024-01-24" version="1">MEQTELVFIPFPMIGHLASALEIAKLITERDPRFSITIIIMKYPLESIDGMDTDSDSIRFVTLPPVEVSSSTTPSGFFLSEFLKANIPIVRDAIHELTRSNSVRLAGFVVDMFCTHMIDVADEFGVPSYLFFTSSAAFLGFLLHLQFLHDYEGLDFNEFKDSDAELDVPSFVNSVPGKVFPSRMFDKESGGAEMLLYHTRRFREVKGILVNTFIELESHAVRSLSGSTVPAVYPVGPILNTRMGSGGGQRDASVIMSWLDDQPPSSVVFLCFGSRGSFGADQIKEIAHGLEHSGHRFLWSLRQPPPKGKMDFPSDYESIEEVLPEGFLHRTARIGKVIGWAPQVAVLSHSAVGGFVSHCGWNSTLESVWYGVPVATWPIYAEQQINAFQMVKDLGLAVEIKIDYNKDRDHVVSAHEIENGLRNLMNTNSEVRQKRKEMQKISRRVMIDGGSSHFSLGHFIEDMVANIPCKQQRRDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DKB3</accession>
    <name>A0AA39DKB3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Disease resistance RPP13-like protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_016092</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056789">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766:SF40">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766">
        <property type="entry name" value="PLANT BROAD-SPECTRUM MILDEW RESISTANCE PROTEIN RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25019">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0611">Plant defense</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="7">
        <location>
            <begin position="10"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="6">
        <location>
            <begin position="183"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="8">
        <location>
            <begin position="435"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="domain" description="R13L1/DRL21-like LRR repeat region" evidence="9">
        <location>
            <begin position="690"/>
            <end position="814"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF25019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9687461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1335" mass="151618" checksum="1DDA269CC88700C8" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DPF5</accession>
    <name>A0AA39DPF5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Disease resistance protein RPM1-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_011327</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DPF5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14798">
        <property type="entry name" value="RX-CC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038005">
        <property type="entry name" value="RX-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF1205">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RPM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="6">
        <location>
            <begin position="5"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="5">
        <location>
            <begin position="157"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="7">
        <location>
            <begin position="407"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="8">
        <location>
            <begin position="521"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="24"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9692198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="882" mass="100959" checksum="8DA8C6550C8D2468" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DRG0</accession>
    <name>A0AA39DRG0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF4005 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_010145</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Binds to multiple calmodulin (CaM) in the presence of Ca(2+) and CaM-like proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IQD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DRG0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025064">
        <property type="entry name" value="DUF4005"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32295">
        <property type="entry name" value="IQ-DOMAIN 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32295:SF274">
        <property type="entry name" value="PROTEIN IQ-DOMAIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13178">
        <property type="entry name" value="DUF4005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4005" evidence="5">
        <location>
            <begin position="369"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="277"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="313"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="18"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="29"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="60"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="286"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="315"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="460"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9694511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="477" mass="52460" checksum="849C8BA1109AAD84" modified="2024-01-24" version="1">MGRKGNWLSSVKKALSPEPKEKKDQRADKSKKKWFGKHKYPDPNPSSLEPVPEPSLAPPEEVKTIEPDNEHHKHVYSVAATTNMASLDVPETDVEVVEITTLTQSSGKAKEEAAAIKIQTAFRGYLARRALRALRGLVRLQSLIQGPAVKRQAANTLRCMQTLARVQSQICSRRIRMSEENQALQRQLLQKQAFEQLKMGEEWDDSLQSKEQIEAGLLNKQEAAMRRERALAYAFSHQQAWKNSSKSTNLLFMDPSNPHWGWSWLERWMAARPWESRSTTDKELNNDQSSIKSGSRSTTGGEITKAYARHLLDSSKPSPTASQKPNHPPARQSPSTPPLKAVSSSSAAGKFKPLASPRGNLWGQDDDTKSMASMQSERFRRHSIAGSSVGDDESLASSPAVPSYMAPTKSAKAKSRLQSPLGVENNGTPEKGSSGIAKKRLSFPASPARPRRHSGPPRVESSSLTESIVSNGGGTVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DRZ5</accession>
    <name>A0AA39DRZ5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">intramembrane prenyl-peptidase Rce1</fullName>
            <ecNumber evidence="10">3.4.26.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_010563</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>Hydrolyzes the peptide bond -P2-(S-farnesyl or geranylgeranyl)C-P1'-P2'-P3'-COOH where P1' and P2' are amino acids with aliphatic sidechains and P3' is any C-terminal residue.</text>
            <dbReference type="EC" id="3.4.26.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase U48 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.26.1" evidence="10"/>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DRZ5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071586">
        <property type="term" value="P:CAAX-box protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039731">
        <property type="entry name" value="Rce1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003675">
        <property type="entry name" value="Rce1/LyrA-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13046:SF0">
        <property type="entry name" value="CAAX PRENYL PROTEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13046">
        <property type="entry name" value="PROTEASE U48 CAAX PRENYL PROTEASE RCE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02517">
        <property type="entry name" value="Rce1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="14"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="93"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="171"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="222"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="249"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="275"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="CAAX prenyl protease 2/Lysostaphin resistance protein A-like" evidence="12">
        <location>
            <begin position="156"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9695126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="316" mass="35594" checksum="365727E472957A5B" modified="2024-01-24" version="1">MEEDAGDGSVTKSVAMMACTAMALFYVAILYSPTWILRLPPPSSFKQFMIRRFICAAISSLVSMLLSALILLPMRSWEVSHLFGVYGIRIDHIWQAMVFPLSLTSLMYAGSLVLKSLLIVSSWKQYKNQGRGIAIDYVKNVPQNIFNWVCSTASSILVWRNYVVAPFTEELVFRACMIPLLLCGGFKTYSVIFLCPIFFSLAHLNHLLEFYSKHNRSLLKAFMVAGLQLGYTVIFGSYASLLFIRTGHIVAPIVAHIFCNFMGLPVLFARGKGMVSVAFVAGMVGFLWLLFPVTDPALYNYRTDNCRCWHGYCTWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DSB7</accession>
    <name>A0AA39DSB7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">MADS-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_009692</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9693850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DSB7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00266">
        <property type="entry name" value="MADS_SRF_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033897">
        <property type="entry name" value="SRF-like_MADS-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11945:SF387">
        <property type="entry name" value="AGAMOUS-LIKE MADS-BOX PROTEIN AGL80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11945">
        <property type="entry name" value="MADS BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00404">
        <property type="entry name" value="MADSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="6">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9693850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="532" mass="57070" checksum="21A32A3AFFE26D89" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DSD5</accession>
    <name>A0AA39DSD5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">GPI mannosyltransferase 1</fullName>
            <ecNumber evidence="11">2.4.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">GPI mannosyltransferase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_011501</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalytic subunit of the glycosylphosphatidylinositol-mannosyltransferase I complex which catalyzes the transfer of the first mannose, via an alpha-1,4 bond from a dolichol-phosphate-mannose (Dol-P-Man) to the glucosaminyl acyl phosphatidylinositol (GlcN-(acyl)PI) intermediate to generate alpha-D-Man-(1-&gt;4)-alpha-D-GlcN-(1-&gt;6)-(1-radyl,2-acyl-sn-glycero-3-phospho)-2-acyl-inositol and participates in the sixth step of the glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Endoplasmic reticulum membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the PIGM family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="11"/>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DSD5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990529">
        <property type="term" value="C:glycosylphosphatidylinositol-mannosyltransferase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051751">
        <property type="term" value="F:alpha-1,4-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004376">
        <property type="term" value="F:GPI mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007704">
        <property type="entry name" value="PIG-M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12886:SF0">
        <property type="entry name" value="GPI MANNOSYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12886">
        <property type="entry name" value="PIG-M MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05007">
        <property type="entry name" value="Mannosyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4 11" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="6 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041394484" description="GPI mannosyltransferase 1" evidence="12">
        <location>
            <begin position="22"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="155"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="229"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="300"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="331"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="391"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU365064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9692467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="438" mass="50784" checksum="0FC1DD547CBB5B03" modified="2024-01-24" version="1">MDVRSLLLFSALLRVLLIIYGEWQDAHMEVRYTDVDYLVFSDAASLVASGKSPYQRSTYRYSPLLAFLLVPNSIIHPSWGKFLFSASDLLVGLFIHTILELRKVPENLCACALMVWLFNPFTFSIGTRGNCEPIVCAMILWIIICLMNGKVLQAAFWYGLVVHFRIYPIIYSIPIILVLDPHCFRYGLKPALSSWVGQGNSTQSIPSTAVPDSCGIWIALTSMFTRARILFGLISGAVFFAFTGLFFYLYRWEFLHEALLYHLTRTDPRHNFSIYFYHIYLHYEHEFSVVERLISFLPQFLVQLVLIICFAQDLPFCFFAQTVAFVAFNKVITAQYFVWFFCLLPLILPWSNMKLKWTGLSCILLWMGTQTHWLTWGYLLEFKGKNVFLELWIASILFLAANTFVLIKIIRYHTYSPLFQRLEFSSSDNDLREGKKSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DUI7</accession>
    <name>A0AA39DUI7_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_010220</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DUI7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9694642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="210" mass="23529" checksum="DC03397AA561F4A7" modified="2024-01-24" version="1">MFAPSHSTTHNLPLSWSSLHHPLNLHPSIPIFTTVKPIFPANPLHVRQFDNQAPPANHKGTDPKPCKRQTVEDFEHERPIWKEQLSWTIVSLICSKTLSKTSLMVLMGTDAVMGCSVKLHVERIGNKILLCRVPSLVGNVWLIFTKGDLKGISIEDHMFRWIPGNLNRDHLGKHQSGPEKLSLPIVPCASVSLQSLNEIVKLVKQRGAWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DVA2</accession>
    <name>A0AA39DVA2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Response regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_010733</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Functions as a response regulator involved in His-to-Asp phosphorelay signal transduction system. Phosphorylation of the Asp residue in the receiver domain activates the ability of the protein to promote the transcription of target genes. Type-A response regulators seem to act as negative regulators of the cytokinin signaling.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ARR family. Type-A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DVA2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009736">
        <property type="term" value="P:cytokinin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17581">
        <property type="entry name" value="REC_typeA_ARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045279">
        <property type="entry name" value="ARR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43874">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43874:SF167">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR ARR9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0932">Cytokinin signaling pathway</keyword>
    <keyword evidence="1 8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="Response regulatory" evidence="10">
        <location>
            <begin position="10"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="143"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="146"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="176"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="198"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="8">
        <location>
            <position position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00043855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9695407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="219" mass="24924" checksum="C7EE41E660CCBDFD" modified="2024-01-24" version="1">MGMATESQFHVLAVDDSLIDRKLIEKLLKNSSYQVTTVDSGSKALEFLGLHENDPNTPSVSPNSHQEVEVNLIITDYCMPGMTGYDLLKKIKESSSLRNIPVVIMSSENVPSRITRCLEEGAEEFFLKPVQISDVNRLKPHMMKAKNKDQQQENQEKQEKQDEESEETEKSEPGSQQPQQQHQQQQQQQQQSNNNKRKAIEEGLSPDRTRPRYSGLTVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DVI0</accession>
    <name>A0AA39DVI0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Tyrosine decarboxylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_009011</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6 7">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the group II decarboxylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DVI0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1340.10:FF:000001">
        <property type="entry name" value="Histidine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1340.10">
        <property type="entry name" value="dopa decarboxylase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010977">
        <property type="entry name" value="Aromatic_deC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021115">
        <property type="entry name" value="Pyridoxal-P_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11999">
        <property type="entry name" value="GROUP II PYRIDOXAL-5-PHOSPHATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11999:SF70">
        <property type="entry name" value="MIP05841P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00800">
        <property type="entry name" value="YHDCRBOXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00392">
        <property type="entry name" value="DDC_GAD_HDC_YDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="5 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 6 7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="6">
        <location>
            <position position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR602129-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9697060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="396" mass="44341" checksum="ECBF8B9D46D1C2AC" modified="2024-01-24" version="1">MDAEQLRENGHKMVDFIADYYKSIENFPVLSQVEPGYLRELLPDSAPHQPESLQQVFDDLQAKILPGVTHWQSPNFFAYYPSNSSTAGFLGEMLSAGLNIVGFSWITSPAATELEMIVLDWLAKMLNLPDDFLSAGNGGGVIQGTASEAVLVVLLAARDRVLRTVGKTALEKLVVYGSDQTHSALQKACQIGGIHPENCRLLKADSSTGYALSPDLLSEAVSHDIANGLIPFFLCANSNGIWFHVDAAYAGSACVCPEYRHYIDGVEEADSFNMNAHKWFLTNFDCSVLWVKASDANMVVDYKDWQVPLGRRFRSLKLWMVLRLYGVEKLQHYIRNHIELAKRFEELVAQDPRFEALSGKYTLRLAVGAPLTEERHVNAAWRLFKRKPLFFYQSLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DXH8</accession>
    <name>A0AA39DXH8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Sn1-specific diacylglycerol lipase alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_005505</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9699669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DXH8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00519">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002921">
        <property type="entry name" value="Fungal_lipase-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005592">
        <property type="entry name" value="Mono/diacylglycerol_lipase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46023:SF6">
        <property type="entry name" value="LIPASE CLASS 3 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46023">
        <property type="entry name" value="LIPASE CLASS 3 PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03893">
        <property type="entry name" value="Lipase3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01764">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mono-/di-acylglycerol lipase N-terminal" evidence="4">
        <location>
            <begin position="48"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Fungal lipase-type" evidence="3">
        <location>
            <begin position="182"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="27"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="416"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="432"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9699669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="636" mass="69582" checksum="0BE37EFD9ACEB713" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DYU1</accession>
    <name>A0AA39DYU1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_003897</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9701873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DYU1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47447">
        <property type="entry name" value="OS03G0856100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47447:SF22">
        <property type="entry name" value="TETRATRICOPEPTIDE-LIKE HELICAL DOMAIN SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13812">
        <property type="entry name" value="PPR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="64"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9701873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="118" mass="13084" checksum="90B99E6D7CCDDA49" modified="2024-01-24" version="1">MPFGLPSVRSGPNLAAIKKGNCQTGQSKHGNGVDDQFRVLCMHGNLSQSMQLLDKSVKEGWVPNVFTYSFLLEAANKEREVEKAIRLLDEMTAEGGKPNLVNYNVLLTRPTGRYQQFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DZI4</accession>
    <name>A0AA39DZI4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ubiquitin domain-containing protein DSK2a-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_005257</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DZI4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14399">
        <property type="entry name" value="UBA_PLICs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16106">
        <property type="entry name" value="Ubl_Dsk2p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.100:FF:000005">
        <property type="entry name" value="Ubiquitin domain-containing protein DSK2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000042">
        <property type="entry name" value="Ubiquitin domain-containing protein DSK2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000183">
        <property type="entry name" value="Ubiquitin domain-containing protein DSK2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006636">
        <property type="entry name" value="STI1_HS-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015496">
        <property type="entry name" value="Ubiquilin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10677">
        <property type="entry name" value="UBIQUILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10677:SF53">
        <property type="entry name" value="UBIQUILIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00627">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23195">
        <property type="entry name" value="UBQLN1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00727">
        <property type="entry name" value="STI1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="3">
        <location>
            <begin position="19"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="2">
        <location>
            <begin position="508"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="299"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="459"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="93"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="299"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="459"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9703901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="556" mass="58992" checksum="F8E0DD2BF505FA87" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E1D0</accession>
    <name>A0AA39E1D0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pre-mRNA-splicing factor 38</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_004715</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required for pre-mRNA splicing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the PRP38 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E1D0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005037">
        <property type="entry name" value="PRP38"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024767">
        <property type="entry name" value="PRP38_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23142">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR 38A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03371">
        <property type="entry name" value="PRP38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12871">
        <property type="entry name" value="PRP38_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5 7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Pre-mRNA-splicing factor 38 C-terminal" evidence="9">
        <location>
            <begin position="186"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="176"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="185"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="253"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="393"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="438"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9703060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="458" mass="53976" checksum="AF8754D00335C2BA" modified="2024-01-24" version="1">MEIQTSGKPIDSLLEKVLCMNILSSDYFKELYRLKTYHEVIDEIYNQVDHVEPWMTGNCRGPSTAFCLLYKFFTMKLTVKQMHGLLKHPDSPYIRAVGFLYLRYAGDPKTLWNWFEPYVKDEEEFSPGSTGRMTTMGAYVRDLLLGQYYFDTLFPRIPVPIMRQIVSNLEKMKLPSKHAGVTGDSTRHGSDDTARRPPSVKAALSVSFGQRAPHRASTRDSSPVRRTLPPPPPSYDRNSSDDLRRSPSSRRSQSRELSDREYSDRDRDRGRSRELDRDRDHDRERDRERDRTRNRDKDRDRAMDRDRDRDKDRDRDRDRDRDRERDKDKDRDRERERDRDRRHDYDRRSKYTERESRRDYDRSSRDGSRYNRETSSRRSRSRSRSRSQSLQARNDHIDHRSSPLADGSKEKTSASANLAKLRDLYGDTGDQKGGAMERGTHRDNSGEEVIRLGGSSWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E1J1</accession>
    <name>A0AA39E1J1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_004860</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E1J1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9703240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="420" mass="47370" checksum="8216C68BB66D2282" modified="2024-01-24" version="1">MEGKGVVLIATKQRLLFFDVTKLEDLPYTDTEVPTLPLPVVKEFNFVEKGFPFGAGFIVWESKLYMVGGEKTRGGLSEEELCTPSNNNLDKSFGTRGLSPYIYFSDLTKLDSITEFRKHSLTMLAPKTSPIIEEIEGKIYVLSGPACHYEGLLPNPSFEAYDPSLDQLEALDLPHFYRENRYSDPEYIVHGHSVVGTDIVVLAGEQYYYYIVKDRNWELGETPIPRPLLPGYSGKFVPAYDDIFICFRLHALFAAKVPSDAGPTLIQFLDEVYKDNFFLDLDSLRGVVVAIGEYEMCIIRPKIVNINNVIIPRIYVATFLVEKLDSSAPAPKRAKIASDSQTFHDNHGNYSDRLSRIQDSDDSEPFFLSVASTPFLSVTCLSKGFYTLPEQWGHEKPQICSAFFHPWSVFTLVSLCTLLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E8C9</accession>
    <name>A0AA39E8C9_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_000925</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E8C9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36034">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36034:SF2">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="152"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="371"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="416"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="749"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="371"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="382"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="421"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9709210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="868" mass="94579" checksum="F29F8737B34D2CFD" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E8K0</accession>
    <name>A0AA39E8K0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_002656</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E8K0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03213">
        <property type="entry name" value="ABCG_EPDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000337">
        <property type="entry name" value="ABC transporter G family member 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF111">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="414"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="443"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="486"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="521"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="553"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="629"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="54"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9707707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="657" mass="73497" checksum="60F2A0A4256A955A" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YJD7</accession>
    <name>A0AA38YJD7_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_025313</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YJD7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33785">
        <property type="entry name" value="OS06G0550800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33785:SF5">
        <property type="entry name" value="SERINE_ARGININE REPETITIVE MATRIX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="202"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="211"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9671551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="374" mass="41936" checksum="15705DCD3BF1601C" modified="2024-01-24" version="1">MEVMGDDPFGSFSIRDEKVEAVDLLEECWFFENLLERRKMMSRCYSDPCTSSNFCQEMMVRNSYANDHSPPEKLPEGDDSVGHTSPSLPCLGRRGSTQEEGPGSGPSKSTQKPTQNHFLGSPSLPPSVGRREGEIQVKDVDLVQPNLLRAPSLPPCVGKKEGAVQEGEIRLGTGKLTQKSSQRGLVRAPSMPPCVGREGLIQYEERDPRSSKLTRTTSLSPPDILPRRHTSKGLAQNYGTPRHRLPRQPEEGTMSMDVAKEMRRRYLNQTKVIRKSLNDLEYEEVQGFKDLGFTFEKEDLSPSVVNILPGLQDKDREGPMEEDSVKRPYLSEAWIEQCSAPPIPNWVGKSSAQDMKTQIKFWARAVASNVRQEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YJN5</accession>
    <name>A0AA38YJN5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">3-deoxy-manno-octulosonate cytidylyltransferase</fullName>
            <ecNumber evidence="7">2.7.7.38</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">CMP-2-keto-3-deoxyoctulosonic acid synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_025386</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>3-deoxy-alpha-D-manno-oct-2-ulosonate + CTP = CMP-3-deoxy-beta-D-manno-octulosonate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23448"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:85986"/>
            <dbReference type="ChEBI" id="CHEBI:85987"/>
            <dbReference type="EC" id="2.7.7.38"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Nucleotide-sugar biosynthesis; CMP-3-deoxy-D-manno-octulosonate biosynthesis; CMP-3-deoxy-D-manno-octulosonate from 3-deoxy-D-manno-octulosonate and CTP: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the KdsB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.38" evidence="7"/>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YJN5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008690">
        <property type="term" value="F:3-deoxy-manno-octulosonate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901137">
        <property type="term" value="P:carbohydrate derivative biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044281">
        <property type="term" value="P:small molecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02517">
        <property type="entry name" value="CMP-KDO-Synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000011">
        <property type="entry name" value="3-deoxy-manno-octulosonate cytidylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00057">
        <property type="entry name" value="KdsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003329">
        <property type="entry name" value="Cytidylyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004528">
        <property type="entry name" value="KdsB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00466">
        <property type="entry name" value="kdsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003950">
        <property type="entry name" value="PRK05450.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003952">
        <property type="entry name" value="PRK05450.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009905">
        <property type="entry name" value="PRK13368.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42866">
        <property type="entry name" value="3-DEOXY-MANNO-OCTULOSONATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42866:SF2">
        <property type="entry name" value="3-DEOXY-MANNO-OCTULOSONATE CYTIDYLYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02348">
        <property type="entry name" value="CTP_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00050198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00060624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00060845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00066873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00082857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9671654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="297" mass="32990" checksum="4B88BE6EA4C12FFD" modified="2024-01-24" version="1">MAVCSSSSSSESSSTTKTWIIHGILTGAAVAVALGAHAYLFLRRSSKFRSRVVGIIPARFASSRFQGKPLVQILGKPMIQRTWERAKLATTLDHVVVATDDDRIADCCRGFGADVIMTSESCRNGTERCNEALQKLEKQYNIVVNIQGDEPLIEPEIIDGVVKALQAAPDAVFSTAVTSLKPEDGFDPNRVKCIVDNHGYAIYFSRGLIPFNKSGKVNQQFPYLLHLGIQSYDTKFLRIYPDLPSTPLQLEEDLEQLKVLENGYKMKVIKVDHEAHGVDTPEDVDKIESFMRERNLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YPY8</accession>
    <name>A0AA38YPY8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_023762</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YPY8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF78">
        <property type="entry name" value="OS12G0587133 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="483"/>
            <end position="763"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9674412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1215" mass="140218" checksum="37455E3DE461A0DE" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YR44</accession>
    <name>A0AA38YR44_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_024175</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YR44"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33133">
        <property type="entry name" value="OS08G0107100 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33133:SF51">
        <property type="entry name" value="THH1_TOM1_TOM3 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="142"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="179"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="228"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="267"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9675120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="332" mass="38142" checksum="47D037A0DB29C797" modified="2024-01-24" version="1">MDLEQEEMQFLGIFGIYKEAYKIIFSWKKIFSQITVALILPLCFIFLAYIEVSEYLSLKIFIHEFELDETRVDSAQHNKISDLLSRELIAFWLLKLAYLVFTLIFSLLSTSAVVYTIACIYTGREVTFKKVMSVVPRVWKRLMVTFLSIFVAVFAYNIVALLILITWALCIGPVKIGILLFWVMFILYLVGLSYLSIIWQLASVVSVLEDLYGFRAMVKGRALIRGKLVVAIVIFLKLNISLVVIKIAFERLVVFGASLGMANRVGYAILCFLLLFTLFLFGLVLQTIIYFVCKSYHHENIDKSALSDHLEVYMGEYVPLKAKDVQLEQYDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YRI1</accession>
    <name>A0AA38YRI1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ABC transmembrane type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_024317</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YRI1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF108">
        <property type="entry name" value="ABC TRANSPORTER C FAMILY MEMBER 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="150"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="8">
        <location>
            <begin position="136"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9675316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="282" mass="31543" checksum="820A016DC2058C5A" modified="2024-01-24" version="1">MDATKYEKAIKPCDLDKDINSFDHGDETKISQRGLNMSGGQKQRIQLMHIQLQFFSMNTSMAALAHKTVILVTHQVEFLSEVDKILVMEARQITQSGSYEELLTSRTSFEYDVGWNPFMDNLLASKWNAPDAASTYWLTLGIRIPNINNTLLIGVYTAISTLSAIFVYLRSFCEAFLGLKASKALFADFTNSIFNAPMLFFDSTPVGRILTQASSDFRVVDFDIPFSIILVVAADIELIITIEMTSSVPFSACGSLMEIVSLTVEDHILESALFFRRIKNDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YRW9</accession>
    <name>A0AA38YRW9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">5-formyltetrahydrofolate cyclo-ligase-like protein COG0212</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_024462</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YRW9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10420:FF:000004">
        <property type="entry name" value="5-formyltetrahydrofolate cyclo-ligase-like protein COG0212"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10420">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002698">
        <property type="entry name" value="FTHF_cligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024185">
        <property type="entry name" value="FTHF_cligase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13017">
        <property type="entry name" value="5-FORMYLTETRAHYDROFOLATE CYCLO-LIGASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13017:SF0">
        <property type="entry name" value="METHENYLTETRAHYDROFOLATE SYNTHASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01812">
        <property type="entry name" value="5-FTHF_cyc-lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="333"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9675541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="354" mass="39114" checksum="DAC9CBE83451024A" modified="2024-01-24" version="1">MDVMIRISSPFAIFNKPHDSPTSFSFSVPFSLNKNVGGSRNYQKLGSSRSSRNDAVFDEAAFEADRLTLDARARESMAEASKRETETDPKAWKWVIRKRVWDFMEARNIAQNPRPVHHRIPNFVGAAAAAQKLGGLEAFGVAKCVKVNPDSPQKQVRFLTLSGGKKLLTPQPRLRTGFFSVLESSMLTPSTINEACTSVGVAKYGRPIGLDEQIKVDLIVIGSVAVDPKTGARLGKGEGFAELEYGMLRYMGAIDDTTPIVTSVHDEQLVDDIPVEKLLIHDVPVDIICTPTQVIYTNTSIPKPQGIYWDKLSPEKLGQIRILRELKSRIEGETGQKLPCGPSEKLPPTAKRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YT76</accession>
    <name>A0AA38YT76_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Terpene synthase metal-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_024981</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9676246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YT76"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010333">
        <property type="term" value="F:terpene synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050148">
        <property type="entry name" value="Terpene_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005630">
        <property type="entry name" value="Terpene_synthase_metal-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225">
        <property type="entry name" value="OS04G0344100 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225:SF241">
        <property type="entry name" value="TERPENE SYNTHASE FAMILY, METAL-BINDING DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03936">
        <property type="entry name" value="Terpene_synth_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Terpene synthase metal-binding" evidence="2">
        <location>
            <begin position="12"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="Terpene synthase metal-binding" evidence="2">
        <location>
            <begin position="46"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9676246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="96" mass="11439" checksum="91B5FC16B40FD100" modified="2024-01-24" version="1">MYKYVTLRNVCLMKIQVECYFDEAQWFHEGHVPTIEEYMQVARVREMITKEAFDWVYNDPFTVASCLIVRLMNDMTTHKQMKANSSFFHRSACNGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YTN0</accession>
    <name>A0AA38YTN0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Terpene synthase metal-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_024994</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9676259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YTN0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010333">
        <property type="term" value="F:terpene synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005630">
        <property type="entry name" value="Terpene_synthase_metal-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03936">
        <property type="entry name" value="Terpene_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Terpene synthase metal-binding" evidence="1">
        <location>
            <begin position="2"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9676259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="50" mass="5736" checksum="6320BFF0A4B63889" modified="2024-01-24" version="1">MTTKEAFDWVYNDPFTVASCLIVRLMNDMTTHKQMKANSSFFHMSACNGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YWN5</accession>
    <name>A0AA38YWN5_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_022841</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9678078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YWN5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9678078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="121" mass="13865" checksum="BD07A4A19E75278C" modified="2024-01-24" version="1">MGLGESLDQSLRGGSKKASSMSSNRLPRNYLVYKSSGYIEQSSKKSRVLEAWWDSIDLENFANYHEYEMEFLNNGSDEDQIKIAENLQVRCVNGQEKVHVSASEQSKLYNDDFFQARARTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YY72</accession>
    <name>A0AA38YY72_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="8">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_020773</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. UvrD subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="8"/>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YY72"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000725">
        <property type="term" value="P:recombinational repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17932">
        <property type="entry name" value="DEXQc_UvrD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18807">
        <property type="entry name" value="SF1_C_UvrD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.160:FF:000007">
        <property type="entry name" value="p-loop containing nucleoside triphosphate hydrolase superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.486.10">
        <property type="entry name" value="PCRA, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013986">
        <property type="entry name" value="DExx_box_DNA_helicase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014017">
        <property type="entry name" value="DNA_helicase_UvrD-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000212">
        <property type="entry name" value="DNA_helicase_UvrD/REP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014016">
        <property type="entry name" value="UvrD-like_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070:SF61">
        <property type="entry name" value="DNA 3'-5' HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070">
        <property type="entry name" value="UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00580">
        <property type="entry name" value="UvrD-helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13361">
        <property type="entry name" value="UvrD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51198">
        <property type="entry name" value="UVRD_HELICASE_ATP_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51217">
        <property type="entry name" value="UVRD_HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 10" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UvrD-like helicase ATP-binding" evidence="12">
        <location>
            <begin position="250"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="domain" description="UvrD-like helicase C-terminal" evidence="13">
        <location>
            <begin position="547"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="380"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="271"/>
            <end position="278"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9678687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1150" mass="128989" checksum="D8C4FE6429B7ACAC" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YZB9</accession>
    <name>A0AA38YZB9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">mitogen-activated protein kinase kinase kinase</fullName>
            <ecNumber evidence="2">2.7.11.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_021415</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.25" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YZB9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004709">
        <property type="term" value="F:MAP kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd06632">
        <property type="entry name" value="STKc_MEKK1_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000186">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050538">
        <property type="entry name" value="MAP_kinase_kinase_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016">
        <property type="entry name" value="MAP KINASE KINASE KINASE SSK2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016:SF45">
        <property type="entry name" value="OS04G0559800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="410"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="284"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="679"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="746"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="877"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="18"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="55"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="164"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="284"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="324"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="373"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="763"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="879"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="439"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9679476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="896" mass="96305" checksum="E409424E956D54B8" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YZK3</accession>
    <name>A0AA38YZK3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">HMA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_021339</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the HIPP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YZK3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051863">
        <property type="entry name" value="HIPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45811">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45811:SF11">
        <property type="entry name" value="METAL-ASSOCIATED DOMAIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0488">Methylation</keyword>
    <keyword evidence="4" id="KW-0636">Prenylation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMA" evidence="7">
        <location>
            <begin position="4"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="72"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="73"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9679378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="106" mass="12023" checksum="840633B572E9BC1B" modified="2024-01-24" version="1">MAQQKVVLKVMTMSDDKTKQKAMEAAADIYGVDSIAADLKEQKLTVIGMMDAVAVVKKLKKVGKVDIISVGPAKEEKKEEKKEEKKEEKKEEKKEEKKEEKKDEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z068</accession>
    <name>A0AA38Z068_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_021746</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z068"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34970">
        <property type="entry name" value="ABC TRANSPORTER A FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34970:SF2">
        <property type="entry name" value="ABC TRANSPORTER A FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9679938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="72" mass="7917" checksum="F6E2F58ECF19B4CB" modified="2024-01-24" version="1">MGFVLRVRLASFFAGAATASFLGLYILHKDYKVAHESISRQMKGLNDSLDARISALEKLKEVEASQSVEATE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z133</accession>
    <name>A0AA38Z133_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_019534</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z133"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053258">
        <property type="entry name" value="Ca-permeable_cation_channel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34115">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34115:SF5">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9680252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="154" mass="17556" checksum="06F7EE3CE7EDB238" modified="2024-01-24" version="1">MIIHDSTLSIHHVIYNVMGINPREQHNLMSIHTATFINNFRKEADFVFQAHPINTKVTILSLLAFVLAFGIELTFHTCHISPTCAALLRTTMVFFGSFSLASLASLLFPGALWSFLLVIYAFLALSNMQNLVQKWCHWVHYRLIYNLETVLTGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z3Y1</accession>
    <name>A0AA38Z3Y1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Germin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_018040</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May play a role in plant defense. Probably has no oxalate oxidase activity even if the active site is conserved.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Secreted</location>
            <location evidence="2 13">Extracellular space</location>
            <location evidence="2 13">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the germin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9681951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z3Y1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02241">
        <property type="entry name" value="cupin_OxOx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000005">
        <property type="entry name" value="Germin-like protein subfamily 1 member 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006045">
        <property type="entry name" value="Cupin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001929">
        <property type="entry name" value="Germin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019780">
        <property type="entry name" value="Germin_Mn-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31238">
        <property type="entry name" value="GERMIN-LIKE PROTEIN SUBFAMILY 3 MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00190">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00325">
        <property type="entry name" value="GERMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00835">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00725">
        <property type="entry name" value="GERMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 13" id="KW-0052">Apoplast</keyword>
    <keyword evidence="7 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 10 13" id="KW-0464">Manganese</keyword>
    <keyword evidence="6 10 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0964">Secreted</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041486277" description="Germin-like protein" evidence="13">
        <location>
            <begin position="24"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="Cupin type-1" evidence="14">
        <location>
            <begin position="64"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="33"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU366015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9681951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="221" mass="23760" checksum="D1E743F60206D639" modified="2024-01-24" version="1">MKKGVSFLVTVALIALVSSLASAYDPSPLQDTCVAIDEPNDAVFVNGKFCKNPNLTMAEDFFSPGLDKPGNTSNQVGSNVTTVNVDKIKGLNTLGISIVRIDYEPYGENPPHTHPRATEILTVLEGTLYVGFVTSNPENRLISKVLNKGDVFVFPIGLIHFQFNVGHTNAVAIAGLNSQNPGVITIAKAVFGSNPPINPDVLTKAFQLDKNVVDYLQSRFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z409</accession>
    <name>A0AA38Z409_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_017851</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9681658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z409"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072354">
        <property type="term" value="F:histone H3T3 kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000401">
        <property type="entry name" value="serine/threonine-protein kinase haspin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000605">
        <property type="entry name" value="Serine/threonine-protein kinase haspin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024604">
        <property type="entry name" value="GSG2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24419">
        <property type="entry name" value="INTERLEUKIN-1 RECEPTOR-ASSOCIATED KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24419:SF18">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE HASPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12330">
        <property type="entry name" value="Haspin_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01331">
        <property type="entry name" value="DUF3635"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="310"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="40"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9681658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="623" mass="70102" checksum="F8D8C82910AB2BD9" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z5A2</accession>
    <name>A0AA38Z5A2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_018453</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057135">
        <property type="entry name" value="At4g27190-like_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050905">
        <property type="entry name" value="Plant_NBS-LRR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463:SF198">
        <property type="entry name" value="RPP4C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23247">
        <property type="entry name" value="LRR_RPS2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0611">Plant defense</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="174"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="1293"/>
            <end position="1431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9682535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="2173" mass="248617" checksum="7AB73523542F6456" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z622</accession>
    <name>A0AA38Z622_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">allene-oxide cyclase</fullName>
            <ecNumber evidence="3">5.3.99.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_018791</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(9Z,13S,15Z)-12,13-epoxyoctadeca-9,11,15-trienoate = (9S,13S,15Z)-12-oxophyto-10,15-dienoate</text>
            <dbReference type="Rhea" id="RHEA:22592"/>
            <dbReference type="ChEBI" id="CHEBI:36438"/>
            <dbReference type="ChEBI" id="CHEBI:57411"/>
            <dbReference type="EC" id="5.3.99.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the allene oxide cyclase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.99.6" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z622"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046423">
        <property type="term" value="F:allene-oxide cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009695">
        <property type="term" value="P:jasmonic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.480.10">
        <property type="entry name" value="Allene oxide cyclase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009410">
        <property type="entry name" value="Allene_ox_cyc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044859">
        <property type="entry name" value="Allene_oxi_cyc_Dirigent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034871">
        <property type="entry name" value="Allene_oxi_cyc_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31843">
        <property type="entry name" value="ALLENE OXIDE CYCLASE 4, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31843:SF11">
        <property type="entry name" value="ALLENE OXIDE CYCLASE 4, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06351">
        <property type="entry name" value="Allene_ox_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141493">
        <property type="entry name" value="Allene oxide cyclase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-0934">Plastid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9682939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="251" mass="27621" checksum="F7F1E6A3C386BE48" modified="2024-01-24" version="1">MASSTPALRSFSSSFKLLNPRSVSSTPAHPLLPFKFSNPSPSHNLTLHSTHYSFPKPARSLSFSCKAQSSPSDPPRPTQVQELHVYEINERDRGSPAYLRLSQKSVNSLGDLVPFSNKIYTGDLEKRLGVTAGICVLIQNVPEKQGDRYEAIYSFYFGDYGHISVQGAYLTYEDTYLAITGGSGIFEGAYGQVKLQQIIFPFKLFYTFYLKGIKELPADLLGKPVSPAPHVEPSQAAKACEPNACIANFTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z692</accession>
    <name>A0AA38Z692_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein DETOXIFICATION</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Multidrug and toxic compound extrusion protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_018688</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z692"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13132">
        <property type="entry name" value="MATE_eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045069">
        <property type="entry name" value="MATE_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="29"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="57"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="102"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="145"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="175"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="203"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="243"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="283"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="324"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="365"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="397"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="427"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="456"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9682812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="481" mass="52029" checksum="C187E2E1662DA062" modified="2024-01-24" version="1">MQLGSREEKRREEVTWGGFAGELKKVGCLAAPMVVATVSQYLLQVASVVMVGHLGQVSLSAVAIATALTNVTGFSLLSGLAGGLETLCGQAYGAHQYPKLGIYTYSATISLALVCLPICLLWIFMDKLLILIGQDPLIALESRNYSIWLIPGLYGSAILKPLVRYLQTQSLIFPMLISSLIILGLHIPICWSLVFKLELGNRGAAVAISISSWLNVVLLGLYVKYSSACEKTRMSFSKDAFFVMGEFFHFAVPAAVMVCLKWWSCELLTLLSGLLPNPKLESSILAICLTITTLHFTIPYGLGAVASTRVSNELGAGNPQAARVAVWAVIFLAIIETTVVSTTLFCCRYVLGYAYSSDKQIVDNVAVMAPLICLSIVMDSIQGVLSGVARGSGWQHIGAYINLGAFYVVGLPVAIILGFVVHLKAKGLWIGIVTGSVVQSTLLSIITGFTNWKKQVLVQILHFSSTFVNFLFHLMLCLVME</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z7D6</accession>
    <name>A0AA38Z7D6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">60S acidic ribosomal protein P0</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_016325</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">Ribosomal protein P0 is the functional equivalent of E.coli protein L10.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">P0 forms a pentameric complex by interaction with dimers of P1 and P2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the universal ribosomal protein uL10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9683776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z7D6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05795">
        <property type="entry name" value="Ribosomal_P0_L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.105.20:FF:000001">
        <property type="entry name" value="60S acidic ribosomal protein P0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.105.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050323">
        <property type="entry name" value="Ribosomal_protein_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001790">
        <property type="entry name" value="Ribosomal_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040637">
        <property type="entry name" value="Ribosomal_uL10-like_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043164">
        <property type="entry name" value="Ribosomal_uL10-like_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043141">
        <property type="entry name" value="Ribosomal_uL10-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030670">
        <property type="entry name" value="uL10_eukaryotes"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45699">
        <property type="entry name" value="60S ACIDIC RIBOSOMAL PROTEIN P0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45699:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00428">
        <property type="entry name" value="Ribosomal_60s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00466">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17777">
        <property type="entry name" value="RL10P_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039087">
        <property type="entry name" value="L10E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160369">
        <property type="entry name" value="Ribosomal protein L10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL10-like insertion" evidence="8">
        <location>
            <begin position="114"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="286"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="286"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR039087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9683776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="320" mass="34224" checksum="B239907BBABE23C3" modified="2024-01-24" version="1">MAVKPSKADKKIAYDQKLCQLLDEYSQILIAAADNVGSNQLQNIRKGLRGDSVVLMGKNTMMKRSIRLHAEKTGNTAFLNLIPLLVGNVGLIFTKGDLKEVSEEVAKYKVGAPARVGLVAPIDVIVPPGNTGLDPSQTSFFQVLNIPTKINKGTVEIITPVELIKKGDKVGSSEAALLAKLGIRPFSYGLVVLSVYDNGSVFSPEVLDLTEEDLIEKFAAGVSMVTSLSLAISYPTLAAAPHMFINAYKNVLAVAVATEYSFPQADKVKEYLKDPSKFAVATAPVTASDAGAAPAASKEEEKKEEPAEESDDDMGFSLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z966</accession>
    <name>A0AA38Z966_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Lysosomal Pro-X carboxypeptidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_016957</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S28 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z966"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008239">
        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070008">
        <property type="term" value="F:serine-type exopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.980:FF:000006">
        <property type="entry name" value="Serine carboxypeptidase S28 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.980">
        <property type="entry name" value="Serine carboxypeptidase S28, SKS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008758">
        <property type="entry name" value="Peptidase_S28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042269">
        <property type="entry name" value="Ser_carbopepase_S28_SKS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11010:SF37">
        <property type="entry name" value="LYSOSOMAL PRO-X CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11010">
        <property type="entry name" value="PROTEASE S28 PRO-X CARBOXYPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05577">
        <property type="entry name" value="Peptidase_S28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041291558" description="Lysosomal Pro-X carboxypeptidase" evidence="6">
        <location>
            <begin position="24"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9684741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="503" mass="55897" checksum="8FB928C89595B969" modified="2024-01-24" version="1">MGTRAVSCLPWLILLFITASVSATPFRKIPRLGVHGGSSLGVLGGSSSLRTVSVNLSENFQTFFYPQTLDHFNYRPESYTTFQHRYMVNFNFWGGARSAAPIFVYLGEESDLDKDINSIGFLVENGARFGALLVYIEHRYYGKSNPFGSMEKSLQNASQRGYFNSGQALADYAEVIINLKKNLSADSSPVIVVGGSYGGLLAAWFRLKYPHVALGALASSAPILYFDDITPQDGYYSLVTKDFRDFSESCYNTIKDSWAEIDKAAAEANGLFNLSKKFRTCKPLESASQLKDYLETMYSVAAQYDRPPRYPVTIVCNGIDGGSQGTDILGRIFSGIVASGGNKSCYDMGQSSFPSETEEGWNWQVCSELAIPIGRGSNDTMFPAAPFDFKEYADSCRYSYGVTPRPHWITSYYGGHNIKLILKRFGSNIIFSNGLRDPYSSGGVLEDISHSIIAVHTPRGSHCLDILPSREDDPNWLVLQRNVEIEIIHGWLLKYYEDLLQSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z9P9</accession>
    <name>A0AA38Z9P9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Retrovirus-related Pol polyprotein from transposon TNT 1-94-like beta-barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_017164</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z9P9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054722">
        <property type="entry name" value="PolX-like_BBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34222">
        <property type="entry name" value="GAG_PRE-INTEGRS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34222:SF95">
        <property type="entry name" value="RRNA 2'-O-METHYLTRANSFERASE FIBRILLARIN-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22936">
        <property type="entry name" value="Pol_BBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Retrovirus-related Pol polyprotein from transposon TNT 1-94-like beta-barrel" evidence="2">
        <location>
            <begin position="324"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="212"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="262"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9685021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="413" mass="45627" checksum="4AF25B7F4DBD803C" modified="2024-01-24" version="1">MAIKNSIFSSVISGPPIITLEKLVGSENYLSWFASVELWFMGQGYEDHLVTQEADIPEVDKVQWRKIDAQLCSVLWQSVDPKFLLHLRAYKTCFKFWNQAKGLYTNDIQRLYKVASSIVNFRQQNMDLSTYIGQITSFKEEFLTVMPLTTDVRDQRIQIDKFFMVLTLIGLRPDLETVRDQILGNSSVPSLDDVFARLLRISSTQTLPFDHTSDSSVLVSQTNSRGGRSGNRGRGQRPHCTYCNKLGHTRDWCYQLHGRPPRTAHVAQSSDPQPPQPLSSSTSQDISLTDSGYDQATQSASIASIAQTGNSSACLTHTSSLGPWILDSGASDHTSGNKDLFSSITTTSALPTVTLANGSQTVAKGIGLAHPLPSLPLTSVLYTPECSFNLISISKITRTLNCSITFSDKFVTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z9R0</accession>
    <name>A0AA38Z9R0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">HECT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_017060</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z9R0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045322">
        <property type="entry name" value="HECTD1/TRIP12-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45670:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HECTD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45670">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="HECT" evidence="5">
        <location>
            <begin position="22"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="3">
        <location>
            <position position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9684887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="66" mass="7022" checksum="374C49432BB9F3C0" modified="2024-01-24" version="1">MVQHSSTTVSTAANGSSGPSESADDDLPSVMTCANYLKLPPYSTKEIMYKKLLYAISEGQGSFDLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZAD1</accession>
    <name>A0AA38ZAD1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_016996</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZAD1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48017">
        <property type="entry name" value="OS05G0424000 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="65"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="111"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="154"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="183"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="223"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="263"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="303"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="355"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="381"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="410"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="8">
        <location>
            <begin position="30"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9684794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="452" mass="49339" checksum="66D4346BAE11618F" modified="2024-01-24" version="1">MEAGLSHSMEDGTNPPKPLDAGALFVLKSKGSWWHCAYHLTTSIVSPAILSLPFALSLLGWVAGVFFLTMTALVTFYSYNLLSVVLEHHAQLGQRQLRFRDMATDILGPGWGRYLVGPIQIGLCYGTVIAGVLIGGQSLKFIYLLSRPNGTMQLYQFVIISGVLMLVLVQIPSFHSLRHINLVSLVLCLSFCASATAGSIYIGHSKTASVKNYSVHGSGEHRLFGALNAISIIATTYGNGVIPEIQATIAPPVKGKMFKGLCVCYAVVLTTFFSVAISGYWAFGNQAKGTVLANFMVDEKPLLPSWVHLMTNVFTFLQVSAVSLVYLQPTNEVLEKKFADPKIDQFAVRNVMPRLVFRSFSVVIATTLAAMLPFFGDINAVLGAFGFIPLDFILPMIFYNVTFKPSKQSLIFWGNTLLAILFSALGALAAISSIRQIILDANTYRLFANIQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZAV0</accession>
    <name>A0AA38ZAV0_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_017473</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZAV0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35482">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35482:SF1">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9685445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="104" mass="12036" checksum="D4EDD06A906B3E47" modified="2024-01-24" version="1">MVDKPDEARVMYERLQTHPNALVSKKAKKFMFGFQAMEMVKITSQLPSKNTGYQNFFETFIEDKVDYSLKDNEVEVEHCQALPYIIFLVSPIFIVLLIALQKGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZB71</accession>
    <name>A0AA38ZB71_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_017629</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZB71"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9685669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="119" mass="13637" checksum="828CA41C2F3DA780" modified="2024-01-24" version="1">MAAALLIGKTFHTKNRSIQELRDRPNPRIPTFLCCQNQGDIDELESKTVKGKENRPLVLRVFAGVAKFGEGLRENLSPKQKGDWKDLVLMSLSFAVYVYMSQRIVCAYCVWMSMVKQPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZC36</accession>
    <name>A0AA38ZC36_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_015159</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZC36"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13999">
        <property type="entry name" value="STKc_MAP3K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000060">
        <property type="entry name" value="Serine/threonine-protein kinase isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055164">
        <property type="entry name" value="EDR1/CTR1/ARMC3-like_pept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050167">
        <property type="entry name" value="Ser_Thr_protein_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23257:SF821">
        <property type="entry name" value="ATP BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23257">
        <property type="entry name" value="SERINE-THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14381">
        <property type="entry name" value="EDR1_CTR1_ARMC3_pept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="528"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="273"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="365"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="467"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="296"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="555"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9686134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="784" mass="87991" checksum="BA19B3B5F74F0B67" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZD71</accession>
    <name>A0AA38ZD71_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NADH:ubiquinone reductase (non-electrogenic)</fullName>
            <ecNumber evidence="5">1.6.5.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PVL29_015622</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a quinone + NADH + H(+) = a quinol + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:46160"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.6.5.9"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>a ubiquinone + NADH + H(+) = a ubiquinol + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:23152"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Intermembrane side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the NADH dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.6.5.9" evidence="5"/>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZD71"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050136">
        <property type="term" value="F:NADH dehydrogenase (quinone) (non-electrogenic) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.100:FF:000002">
        <property type="entry name" value="External alternative NAD(P)H-ubiquinone oxidoreductase B1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.100:FF:000008">
        <property type="entry name" value="External alternative NAD(P)H-ubiquinone oxidoreductase B1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045024">
        <property type="entry name" value="NDH-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054585">
        <property type="entry name" value="NDH2-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43706:SF3">
        <property type="entry name" value="EXTERNAL ALTERNATIVE NAD(P)H-UBIQUINONE OXIDOREDUCTASE B1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43706">
        <property type="entry name" value="NADH DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22366">
        <property type="entry name" value="NDH2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-0520">NAD</keyword>
    <keyword evidence="11" id="KW-0521">NADP</keyword>
    <keyword evidence="13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="EF-hand" evidence="20">
        <location>
            <begin position="381"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00049010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAJ9686851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="580" mass="65282" checksum="F2FB7133B6A69B00" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZED5</accession>
    <name>A0AA38ZED5_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_016008</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZED5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021475">
        <property type="entry name" value="Pants/Emi1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28052">
        <property type="entry name" value="UPF0545 PROTEIN C22ORF39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28052:SF1">
        <property type="entry name" value="UPF0545 PROTEIN C22ORF39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11326">
        <property type="entry name" value="PANTS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9687347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="107" mass="12535" checksum="9C9FC76711759B59" modified="2024-01-24" version="1">MTSEGEGQAASAPKRRLSCKTCIDNLWFCYSPVHQMQQYYRLGVLDNCADKWSSLLDCLSLKTKRSSEVQEILENREKDKPHIWTFRTSEESSSHWKEMFGHLDDME</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZGE3</accession>
    <name>A0AA38ZGE3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_013868</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9687839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZGE3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080147">
        <property type="term" value="P:root hair cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000017">
        <property type="entry name" value="Transcription factor bHLH66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045843">
        <property type="entry name" value="IND-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16223:SF215">
        <property type="entry name" value="OS02G0564700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16223">
        <property type="entry name" value="TRANSCRIPTION FACTOR BHLH83-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="7">
        <location>
            <begin position="302"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="284"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="464"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="473"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9687839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="541" mass="57311" checksum="73F48B4D983C80A7" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZI71</accession>
    <name>A0AA38ZI71_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_014866</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the ABC transporter superfamily. ABCI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9689387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZI71"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03260">
        <property type="entry name" value="ABC_PstB_phosphate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001209">
        <property type="entry name" value="ABC transporter, ATP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005670">
        <property type="entry name" value="PstB-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43423">
        <property type="entry name" value="ABC TRANSPORTER I FAMILY MEMBER 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43423:SF1">
        <property type="entry name" value="ABC TRANSPORTER I FAMILY MEMBER 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="33"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00061382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9689387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="267" mass="29204" checksum="F79A850680752C39" modified="2024-01-24" version="1">MSSATTLILDSSSAVEPLILQVEEEDGERKPKFRVRNLTKVSESGEAILRGIDVDVPKGAIVGVIGPSGSGKSTLLRVLNRLWEPPSGTVYLDGTDIRELDVLGLRRKVGMLFQLPVLFEGTIADNIRYGPQLRGKKLTDNEVLKLLTLADLDSSFMKKTGGELSVGQAQRVALARTLANEPEVLLLDEPTSALDPISTQNIEDVIVKLKKSRGMTIVMVSHSIKQIQRIADMVCLLVDGEIIEVLEPDKLSEARHPMALRFLQLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZJX1</accession>
    <name>A0AA38ZJX1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_012868</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZJX1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36053:SF1">
        <property type="entry name" value="OS04G0680300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36053">
        <property type="entry name" value="OSJNBB0017I01.18 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="135"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9690438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="175" mass="19761" checksum="F3E762C5530200CB" modified="2024-01-24" version="1">MRLLRPPKTRSSFVYLPVRDRDIEGKRSFVEGTMIPDQWTSPCGNQCTKKYAAMQIAWRVFCKKGCYADGETWEECLEECNEICYKDPVFKDQQWSAYIDRSPGSPSHSKECFNACISGCGFKFEISPEEVAKVRSKRSPEALTVQEPPPSTPMPMPTPTDRPCATTEDVPCTSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZL51</accession>
    <name>A0AA38ZL51_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_013333</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZL51"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000774">
        <property type="term" value="F:adenyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.120">
        <property type="entry name" value="BAG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039773">
        <property type="entry name" value="BAG_chaperone_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036533">
        <property type="entry name" value="BAG_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003103">
        <property type="entry name" value="BAG_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12329">
        <property type="entry name" value="BCL2-ASSOCIATED ATHANOGENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12329:SF17">
        <property type="entry name" value="OS04G0619900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02179">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63491">
        <property type="entry name" value="BAG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="3">
        <location>
            <begin position="52"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9691111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="256" mass="28647" checksum="7C1AD9DC30396E36" modified="2024-01-24" version="1">MMKKRSDYSGCMSEGSATTTSSTSKDEEIEWEMRPGGMLVQKRTEKSDSLAPNLLIRVAYGALRYEISVNSQATFGELKKLLTAETGLQPGEQRLIFRGKERENGDYLDMCGVKDRSKVILMEDPSSRERRAIEMRRNAKIQTAHRAISDVSMEVDKLAEQVSAIEKSISNGTKVPEVQITTLIEMLMRQAIKLDGIAAEGDASAQKNLQGKRVQKCVETLDVLKISNGKVKPVIVTTKWETFDPPPTTAHWEFFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZL52</accession>
    <name>A0AA38ZL52_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">C3HC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_013312</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZL52"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013909">
        <property type="entry name" value="NuBaID_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012935">
        <property type="entry name" value="NuBaID_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15835:SF16">
        <property type="entry name" value="F20D23.9 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15835">
        <property type="entry name" value="NUCLEAR-INTERACTING PARTNER OF ALK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08600">
        <property type="entry name" value="NuBaID_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07967">
        <property type="entry name" value="zf-C3HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3HC-type" evidence="7">
        <location>
            <begin position="79"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="NuBaID C-terminal" evidence="8">
        <location>
            <begin position="864"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="391"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="454"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="726"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="936"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="14"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="397"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="411"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="755"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="778"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="954"/>
            <end position="963"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9691080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="963" mass="102313" checksum="CBFD34C99FD0A45E" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZPL2</accession>
    <name>A0AA38ZPL2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Aspartate racemase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_011605</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZPL2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047661">
        <property type="term" value="F:amino-acid racemase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1860">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015942">
        <property type="entry name" value="Asp/Glu/hydantoin_racemase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001920">
        <property type="entry name" value="Asp/Glu_race"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21198:SF7">
        <property type="entry name" value="ASPARTATE-GLUTAMATE RACEMASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21198">
        <property type="entry name" value="GLUTAMATE RACEMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01177">
        <property type="entry name" value="Asp_Glu_race"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53681">
        <property type="entry name" value="Aspartate/glutamate racemase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="43"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9692615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="337" mass="36964" checksum="D9EB3A86F0F5761B" modified="2024-01-24" version="1">MFDGSMAVSFHSLKYPSHMMGNVHKHRILYRTMKNPILAVPPSSTLLQTDESGNLPESKNSSGSDAASTNSDSAGLLINQATTVGIIGGVSVDSTLNFLKKLVQWSSKDGGDSLPFVLCSDPVLNKQLLSHERSHFPLLSTKRESSRMDHTPIVGNLQSKRAFLERSGVQCIVMPCHISHSWHDEISRGCSVPFLHMGECVARELKNAKLKPLEAGSPLRIGVLATNATLTAGFYQEKLQREGFEVVLPDKATMEHTVIPAIEALDRKDMEGARNLLRIALQVLLVRAVNTVILASDDMHDLLPRDDPLLKKCVDPMDALARSTIHWAQSVEKGLDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZQ24</accession>
    <name>A0AA38ZQ24_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_011989</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9693077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZQ24"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9693077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="55" mass="5606" checksum="38929C81937D3DED" modified="2024-01-24" version="1">MDAKGSQQKAPSQNGVPSASASASGVHNFTSGVASNARTHSNAKLTIPCLLHYPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZQ78</accession>
    <name>A0AA38ZQ78_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_011892</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NRAMP (TC 2.A.55) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZQ78"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015086">
        <property type="term" value="F:cadmium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034755">
        <property type="term" value="P:iron ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001046">
        <property type="entry name" value="NRAMP_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11706:SF54">
        <property type="entry name" value="METAL TRANSPORTER NRAMP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11706">
        <property type="entry name" value="SOLUTE CARRIER PROTEIN FAMILY 11 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01566">
        <property type="entry name" value="Nramp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00447">
        <property type="entry name" value="NATRESASSCMP"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="83"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="219"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="250"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="288"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9692974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="319" mass="34568" checksum="1A0AB1BFCF342152" modified="2024-01-24" version="1">MASTPVQPQLIASIGNRSLSNAPLIENPESDQIVVPAVRKLEFFIAFLVLTIAACFFVELGYGKPNSSEVLRGLFVPQLKGHGATGLAISLLGAMVMPHNLFLHSALVLSRKIPRSVPGIKEACRFYMIESGFALMVAFLINVSVISVSGAESCKDLDLNKASFLLRVCLCSCSNMGIYYYHPSISHLASNQIVLKSISNQDSWYVIGTAFALNMLFNIPVWCGVLLTGLSTLVLLALQQYGVRKLEFFIAFLVLTIAACFFVELGYAKPNSSEVLRGLFVPQLKGNGATGLAISLLGAMVMPHNLFLHSALVLSRKIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZQU9</accession>
    <name>A0AA38ZQU9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Cytokinin riboside 5'-monophosphate phosphoribohydrolase</fullName>
            <ecNumber evidence="2 7">3.2.2.n1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_009487</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 7">Cytokinin-activating enzyme working in the direct activation pathway. Phosphoribohydrolase that converts inactive cytokinin nucleotides to the biologically active free-base forms.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>9-ribosyl-trans-zeatin 5'-phosphate + H2O = trans-zeatin + D-ribose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:48564"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16522"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:87947"/>
            <dbReference type="EC" id="3.2.2.n1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 7">
            <text>N(6)-(dimethylallyl)adenosine 5'-phosphate + H2O = N(6)-dimethylallyladenine + D-ribose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:48560"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17660"/>
            <dbReference type="ChEBI" id="CHEBI:57526"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="EC" id="3.2.2.n1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the LOG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.n1" evidence="2 7"/>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9693556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZQU9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016799">
        <property type="term" value="F:hydrolase activity, hydrolyzing N-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009691">
        <property type="term" value="P:cytokinin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005269">
        <property type="entry name" value="LOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031100">
        <property type="entry name" value="LOG_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00730">
        <property type="entry name" value="Rossman fold protein, TIGR00730 family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31223:SF14">
        <property type="entry name" value="CYTOKININ RIBOSIDE 5'-MONOPHOSPHATE PHOSPHORIBOHYDROLASE LOG5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31223">
        <property type="entry name" value="LOG FAMILY PROTEIN YJL055W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03641">
        <property type="entry name" value="Lysine_decarbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102405">
        <property type="entry name" value="MCP/YpsA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0203">Cytokinin biosynthesis</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041217738" description="Cytokinin riboside 5'-monophosphate phosphoribohydrolase" evidence="8">
        <location>
            <begin position="26"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9693556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="185" mass="20744" checksum="EEF990CA15E389B1" modified="2024-01-24" version="1">MCSMKITTRVTRLMFVKLAMGLASGCRILEVEGVKVIANVLQESAPSLEVLEIANMHQRKAEMARHSDCFIALPSGFGTLEELLEVITWAQRGIHDKPVGLLNVDDYYNYLITYIDKAVDDGFIKPSQRHIIVSAPNAKELVQKLEEHVPLHDGVIAKARWMVEQQQPTAYGNLVSIGDLNPRYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZRF1</accession>
    <name>A0AA38ZRF1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Retrovirus-related Pol polyprotein from transposon 17.6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_009743</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9693915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZRF1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.370:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 17.6-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041373">
        <property type="entry name" value="RT_RNaseH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17917">
        <property type="entry name" value="RT_RNaseH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="7">
        <location>
            <begin position="47"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase RNase H-like" evidence="8">
        <location>
            <begin position="233"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase zinc-binding" evidence="9">
        <location>
            <begin position="435"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF17921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9693915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="514" mass="59588" checksum="717CFB4CC0856EF4" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZTN2</accession>
    <name>A0AA38ZTN2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_010537</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZTN2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07346">
        <property type="entry name" value="ABC_6TM_exporters"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001371">
        <property type="entry name" value="ABC transporter ATP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000096">
        <property type="entry name" value="ABC transporter related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221:SF630">
        <property type="entry name" value="ABC TRANSPORTER B FAMILY MEMBER 29, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="210"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="298"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="9">
        <location>
            <begin position="418"/>
            <end position="606"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9695085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="630" mass="69322" checksum="2CFA764F6AA648C0" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZUG6</accession>
    <name>A0AA38ZUG6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">PGG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_010180</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZUG6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd03493">
        <property type="entry name" value="SQR_QFR_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000217">
        <property type="entry name" value="Ankyrin repeat-containing protein ITN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000245">
        <property type="entry name" value="Ankyrin repeat-containing protein ITN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026961">
        <property type="entry name" value="PGG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24186:SF49">
        <property type="entry name" value="ANKYRIN REPEAT FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24186">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13962">
        <property type="entry name" value="PGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 9" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0346">Stress response</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="467"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="509"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="549"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="575"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="9">
        <location>
            <begin position="177"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="9">
        <location>
            <begin position="212"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="9">
        <location>
            <begin position="280"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="9">
        <location>
            <begin position="314"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="9">
        <location>
            <begin position="348"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="PGG" evidence="12">
        <location>
            <begin position="462"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF13962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9694573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="638" mass="68409" checksum="7320F2F26218427D" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZVJ8</accession>
    <name>A0AA38ZVJ8_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_008373</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZVJ8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9696090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="104" mass="11831" checksum="310BB572D0CD73FD" modified="2024-01-24" version="1">MAPKDIKRHVKPSNSFLGKSLRKSFWWYLELRMARNVITEVTEKERPIRDETAKTINPECCSRLSLLLFSFIPPGTLAVVAKATVVISVAATEHEVEVLLSHWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZWN1</accession>
    <name>A0AA38ZWN1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">OTU domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_008559</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C85 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZWN1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22797">
        <property type="entry name" value="OTU_plant_OTU5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.80:FF:000014">
        <property type="entry name" value="Cysteine proteinases superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003323">
        <property type="entry name" value="OTU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050704">
        <property type="entry name" value="Peptidase_C85-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12419:SF10">
        <property type="entry name" value="DEUBIQUITINASE OTUD6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12419">
        <property type="entry name" value="OTU DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02338">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50802">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OTU" evidence="3">
        <location>
            <begin position="171"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="37"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="114"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="56"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="123"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="133"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9696394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="325" mass="36443" checksum="23510E2E07DB3D6A" modified="2024-01-24" version="1">MEDTQEKDGKPPEEFPEDTHEKKRETLDEMLSRHRKEISQLQNKEVAMKKTAAKGSKAEQKSKKKQVEDEISQLSSNLKEKQAAELASIGYSSGSGKEKSNLDNLVKAIAGVSVTSQAEHSRPSKSAKRREKRAQQEAAREQRIQEEQGNIVSDRMIENEILEKKLEPLGLTVNDIKPDGHCLYRAVEDQLALLSGGSSPYSYQELREMVAAYMRKHASDFLPFFLSETEGDSDISLAERFENYCKEIESTAAWGGQLELGALTHCLKKHIMIFSGSFPDVEMGKEYKSDVGTTDTSNQSIMLSYHKHAFGLGEHYNSVIPGLVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZWX4</accession>
    <name>A0AA38ZWX4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">magnesium chelatase</fullName>
            <ecNumber evidence="2">6.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_011023</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>protoporphyrin IX + Mg(2+) + ATP + H2O = Mg-protoporphyrin IX + ADP + phosphate + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13961"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57306"/>
            <dbReference type="ChEBI" id="CHEBI:60492"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.6.1.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Porphyrin-containing compound metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Mg-chelatase subunit H family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.6.1.1" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016851">
        <property type="term" value="F:magnesium chelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015995">
        <property type="term" value="P:chlorophyll biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015979">
        <property type="term" value="P:photosynthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10150">
        <property type="entry name" value="CobN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011771">
        <property type="entry name" value="BchH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003672">
        <property type="entry name" value="CobN/Mg_chltase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022571">
        <property type="entry name" value="Mg_chelatase_H_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02025">
        <property type="entry name" value="BchH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009140">
        <property type="entry name" value="PRK12493.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44119">
        <property type="entry name" value="MAGNESIUM-CHELATASE SUBUNIT CHLH, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44119:SF1">
        <property type="entry name" value="MAGNESIUM-CHELATASE SUBUNIT CHLH, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02514">
        <property type="entry name" value="CobN-Mg_chel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11965">
        <property type="entry name" value="DUF3479"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0149">Chlorophyll biosynthesis</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Magnesium chelatase subunit H N-terminal" evidence="12">
        <location>
            <begin position="80"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="CobN/magnesium chelatase" evidence="11">
        <location>
            <begin position="244"/>
            <end position="1341"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="1335"/>
            <end position="1362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF11965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9695834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1405" mass="156447" checksum="CD1A05C632D23807" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZX05</accession>
    <name>A0AA38ZX05_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_008591</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZX05"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35686">
        <property type="entry name" value="KINETOCHORE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35686:SF1">
        <property type="entry name" value="KINETOCHORE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="306"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9696440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="507" mass="56786" checksum="EBA3FF9AA3CB010F" modified="2024-01-24" version="1">MNFERKQSLNNVDFFFSEEEDWPQNCMSLGGATEKKEGFQIQSQLELLIGTYEADHGRKTENLSHEMQTGFSFEDDDEMPVFDNGGHLILSPRKGSKCNSEEERVYDDEGNKFSTFPLNSGAKNFQKVDLHNFGSEKQDGDTWSAVTKETEELVHLNENAGCSSSHVSYSRGNKFSKGGKTKAKPKFSFRFQSNKEDSFGPFITNEKSSRSSEVDQVPEGLEAIEHKTMEGAIAEFVDGFHGEKLKETEIHAVQGDRTVGHGRSKHSVAELLNDLQEKNGLLGGKSKMCCRRKGRRVQLVIKKNISTSEDRTMQNEDPDEPMASGPSSADEANMQNTRLNVSEAERKTMADRFHDALGAASVNDEAPLFVVPKPSGTGLFGKLQRVMQSEKERDMNFSKKLQIGASPNYEASCIDVKILSRFFEAKLTVCNCSLVENEESSQQGESYQILKECRRRLDTVIFSSRACGDVELEVANLIRIHPPWKEVQVTGSDKIIILATYFSQMSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZXB0</accession>
    <name>A0AA38ZXB0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_008996</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZXB0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06606">
        <property type="entry name" value="STKc_MAPKKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052751">
        <property type="entry name" value="Plant_MAPKKK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48011">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTIONAL COMPLEX SUBUNIT CAF120-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48011:SF56">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="46"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9697035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="383" mass="43222" checksum="5A35F62A47482A99" modified="2024-01-24" version="1">MGMTKRRRDEDDDDYEWMIRRRFPFKFARRIFFHGGSVDNSDGLTWIRTHKLGQGGFGSVYSAKLRSKSPVVFIDDNGGSCIMPSELAVKSAETSKASSLRSEMEILGVLNSSSYVVRCYGDEITRSGDGKLYYNILLEKCWGGSLGSRIKNSGGVGLPENEVRCYTRDIVRGLCYIHGHGYVHCDIKPDNILLVPTCCNGFRAKIGDFGLTKEAYYDPADEDDKGCCGVRGTFRYMSPELLTDNMQCWLSDIWALGCVVIEMMTGKQVWSCEQFEGSDMRDLLRRIAYSPELPEFPSNISAQGRHFLESCLHRDIDERWSALMLLRHPFLSEDGQRSHSILTMHSFSIFNYHSICNSSIASLPCIASQSSLTVIQFVTAYHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZXB6</accession>
    <name>A0AA38ZXB6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="3">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_008338</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclophilin-type PPIase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZXB6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016018">
        <property type="term" value="F:cyclosporin A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01926">
        <property type="entry name" value="cyclophilin_ABH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000022">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase CYP95"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF447">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE CYP63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="7">
        <location>
            <begin position="10"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="180"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="210"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="229"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="268"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="310"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="349"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="374"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="407"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="444"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="554"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="571"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="640"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="674"/>
            <end position="689"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9696029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="704" mass="77878" checksum="86480AB904261A3D" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZXW1</accession>
    <name>A0AA38ZXW1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PVL29_008518</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZXW1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000287">
        <property type="entry name" value="probable LRR receptor-like serine/threonine-protein kinase RKF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47989">
        <property type="entry name" value="OS01G0750732 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47989:SF62">
        <property type="entry name" value="OS05G0423500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 16 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 16 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 17" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="79"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="147"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAJ9696328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="459" mass="50021" checksum="DBBF23CE6C977A04" modified="2024-01-24" version="1">MNITTRSQFDAVVPQTARNDVAVYLTGPSVGNLTDCTAFPSIYAAAFANYLGPTDPGTAECLFSLNFASGGSGNKQRSVVIVVVVVVCSGVFLAVIGGVWVFYRKRRESRDVKREIGNIEMGLESISGSTSLIKFTIDEIRQATRNFTRDNIIGRGGYGNVYKGVLKDGSEVAFKRFKNCSAAGDTNFAHEVEVIASVRHVNLGHQRIIVCDLMKNGSLHDHLFGSTEKLSWPLRQKIALGTARGLAYLHSGAQPGIIHRDIKASNILLDEKFEPKVADFGLAKFTPEGMTHLSTRVAGTMGYVAPEYALYGQLTERSDVYSFGVVLLELLSGKKALMMIGENQPSLVTDWAWSLVRKGRALDVVDNSMPELGSPEVMEKYVLVAVLCSHPQLYARPAMDQVVKILETDLPVPSIPERPIPIVAQMDDIERSVSSSDLGCISSSAGYQSFTFEGQQHSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZYZ9</accession>
    <name>A0AA38ZYZ9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_008912</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the AP2/ERF transcription factor family. ERF subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZYZ9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009873">
        <property type="term" value="P:ethylene-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000001">
        <property type="entry name" value="Ethylene-responsive transcription factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044808">
        <property type="entry name" value="ERF_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31190">
        <property type="entry name" value="DNA-BINDING DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31190:SF469">
        <property type="entry name" value="ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR 1B-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00367">
        <property type="entry name" value="ETHRSPELEMNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="8">
        <location>
            <begin position="93"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="65"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9696913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="222" mass="25099" checksum="7EA59D8214A6C8FA" modified="2024-01-24" version="1">MDSSFFHDQYQTSGFSPEASPFDSLECFSTWDEPLFDHNSLPFNTVDSEEMVLFDMLAEGARDSSETASSAGVVKEDEVNSNAKEATPKNEKLYRGVRRRPWGTFAAEIRDSTRHGRRVWLGTFDTAEAAALAYDQAAFSMRGPMAVLNFSEERVKESLRDIKCGWKDGGSPLVALKRKHLMRRKSACREKKKEKEVREQHVVVLEDLGADYLEQLLCSSQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZZL3</accession>
    <name>A0AA38ZZL3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Auxin-responsive protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_009121</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Aux/IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimers and heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Aux/IAA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZZL3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033389">
        <property type="entry name" value="AUX/IAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003311">
        <property type="entry name" value="AUX_IAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31734:SF257">
        <property type="entry name" value="AUXIN-RESPONSIVE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31734">
        <property type="entry name" value="AUXIN-RESPONSIVE PROTEIN IAA17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02309">
        <property type="entry name" value="AUX_IAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0927">Auxin signaling pathway</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0678">Repressor</keyword>
    <keyword evidence="5 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="PB1" evidence="9">
        <location>
            <begin position="69"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9697199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="110" mass="12276" checksum="0842F8D09A90B051" modified="2024-01-24" version="1">MAALILSRFHSFYSGREALGLCCKRACKCPCYKGTGGRMATSLIFPKEHTMASSAKDNEDAERKSGLGRLYVKVSMDGASYKRKVDLKIYCNYMELSLAMEKMFSCFTIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A1I3</accession>
    <name>A0AA39A1I3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Interactor of constitutive active ROPs 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_007351</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ICR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A1I3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029688">
        <property type="entry name" value="ICR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34224">
        <property type="entry name" value="INTERACTOR OF CONSTITUTIVE ACTIVE ROPS 2, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34224:SF18">
        <property type="entry name" value="INTERACTOR OF CONSTITUTIVE ACTIVE ROPS 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="189"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="474"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="55"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="268"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="397"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9698218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="508" mass="56954" checksum="628C871A98209EC9" modified="2024-01-24" version="1">MSSKLEESQRQLLELSASEEDRVIELQKISQERDRAWESELKAVEKQHSVDSAALASAMSELQQLKTQLEMKVESQVAQTKHTESADSELHSLKENLAETLSLVDNMKNQLKDSKESEAQAQALASETLLQLETAKSTIEALRSDGIKAAETYNSISLEFNESRARVNFLEGLVSKLKTDLTEARSSSLNYAGNDSLDEQQASEHEEIEESNQLKAELASLKIEVGQLKSALDAAEIRYHEEHVQSTVQIRSAYEHVEHVKSASSSRQAELQVELEKTKADIEELKANLMDKETELQGISEENEGLMMKLQNNLSCQREYELEKELKKSKEDVAKLKENLMDKETEFQYVVDENETLKLEMKKREMDKGKFGDGIASEIEAARAAEQEALLKLGYVMEEADKSSRRAARVAEQLEATQVANSEMEAELRKLKVQSDQWRKAAEAAAAMLSAGSNGKLTEKTGSLDSNYNHITGTITSPYSEDLDDESPKKKNGNVLKKIGVLWKKPHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A1L5</accession>
    <name>A0AA39A1L5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Probable magnesium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_007998</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 8">Acts as a Mg(2+) transporter. Can also transport other divalent cations such as Fe(2+), Sr(2+), Ba(2+), Mn(2+) and Co(2+) but to a much less extent than Mg(2+).</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Early endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the NIPA (TC 2.A.7) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9699182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A1L5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008521">
        <property type="entry name" value="Mg_trans_NIPA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12570:SF19">
        <property type="entry name" value="MAGNESIUM TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05653">
        <property type="entry name" value="Mg_trans_NIPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 8" id="KW-0967">Endosome</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="49"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="72"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="149"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="176"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="217"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="241"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="277"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9699182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="350" mass="37861" checksum="D6F29F9453C2286F" modified="2024-01-24" version="1">MGGFSTNNFKGFVLALLSSGFIGASFIIKKKGLRRAAAVSGVRAGVGGYAYLLEPLWWLGMITMIVGEVANFVAYAFAPAVLVTPLGALSIIVSAVLAQFILKERLHKLGILGCVMCISGSVIIVIHAPQERAISSVQEIWNMATQTAFLLYVGSVVVVVFILIFHFAPQCGSTNVLVFTGICSLMGSLSVMSVKALGTSLKLTFEGKNQLIFPETWFFMVVVAICVITQMNYLNKALDTFNTAVVSPIYYVMFTSLTILASVIMFKDWDGQSGGSIISEICGFIVVLSGTILLNVTKDYEGSSFRGIYHPPLSPSLSARLCSGNGELLKHDEENLVSSDEICLRRQELY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A1Q7</accession>
    <name>A0AA39A1Q7_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_007828</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A1Q7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080188">
        <property type="term" value="P:gene silencing by siRNA-directed DNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044567">
        <property type="entry name" value="CLSY/DRD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45821:SF1">
        <property type="entry name" value="ATP-DEPENDENT HELICASE FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45821">
        <property type="entry name" value="SNF2 DOMAIN-CONTAINING PROTEIN CLASSY 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="8">
        <location>
            <begin position="411"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="9">
        <location>
            <begin position="753"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="24"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="35"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9698945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="943" mass="106779" checksum="DDC28A93707D6296" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A1R9</accession>
    <name>A0AA39A1R9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Mitochondrial fission 1 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_007999</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the peroxisomal and mitochondrial division machineries. Plays a role in promoting the fission of mitochondria and peroxisomes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion outer membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Peroxisome membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">The C-terminus is necessary for mitochondrial or peroxisomal targeting, while the N-terminus is necessary for mitochondrial or peroxisomal fission.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the FIS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9699184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A1R9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000266">
        <property type="term" value="P:mitochondrial fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016559">
        <property type="term" value="P:peroxisome fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12212">
        <property type="entry name" value="Fis1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000167">
        <property type="entry name" value="Mitochondrial fission 1 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016543">
        <property type="entry name" value="Fis1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033745">
        <property type="entry name" value="Fis1_cytosol"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028061">
        <property type="entry name" value="Fis1_TPR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028058">
        <property type="entry name" value="Fis1_TPR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13247:SF13">
        <property type="entry name" value="MITOCHONDRIAL FISSION 1 PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13247">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 11 TPR REPEAT PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14853">
        <property type="entry name" value="Fis1_TPR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14852">
        <property type="entry name" value="Fis1_TPR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF008835">
        <property type="entry name" value="TPR_repeat_11_Fis1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 10" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="4" id="KW-0962">Peroxisome biogenesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="141"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR008835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9699184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="167" mass="18223" checksum="A370EF69034936CA" modified="2024-01-24" version="1">MEAKIGKLLDSVGNFFTGADQIPWCDTDIVAGCEREVTEAGKGSSDELKSDCIMRLSWALVHSRRPEDVQRGIAMLEASLAGTNSPLQKREKMYLLAVGYYRSGDYSRSRQLVECCLEIAPDWRQAQTLKKTIEDRIKKDGVIGIGIAATVVGVVVGGIAAAFARKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A3W7</accession>
    <name>A0AA39A3W7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_005753</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A3W7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02859">
        <property type="entry name" value="E_set_AMPKbeta_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001860">
        <property type="entry name" value="Sucrose nonfermenting 4-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032640">
        <property type="entry name" value="AMPK1_CBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050511">
        <property type="entry name" value="AMPK_gamma/SDS23_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780">
        <property type="entry name" value="AMP-ACTIVATED PROTEIN KINASE, GAMMA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780:SF112">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16561">
        <property type="entry name" value="AMPK1_CBM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0129">CBS domain</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="171"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="355"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="437"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9700070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="491" mass="54529" checksum="62DC308E2A4F6A22" modified="2024-01-24" version="1">MLSPGMDSAREGGGVAGTVLIPMNFVWSYGGRSVYLSGSFTGWTNLYQMSPVEGCPTVFQVICSLTPGYHQYKFFVDGEWRHDENQPFISCTYGIVNTVLLARESDYIAPPMSPAVPSLTNMDVDNEAFQQLVRISDGSRHEAVPRIQETDLEVSRHRVSLFLSTHTVYELLPESGKVITLDVDLPVKQAFHILYEQGISIAPLWDYFKGRFVGVLSALDFILILRELGNHGSNLTEEELETHTISAWKEGKGYLNRQIDGNGRAFSRGLIHAGPYDNLKDVALKILENEVATVPIIHSSSEDGSFPQLLHLASLSGILKCICRYFRHSSNSLPVLQLPIFAIPVGTWVSEIGEANQRPLAKLHPSASLSSALSLLVQAQVSSIPIVDDNDSLLDIYSRSDITALAKNRAYAHINLDDMTIHQALQLGQDPYSPYEFNSQRCQMCLRTDPLHEVMDRLANPGVRRLVIVEAGSRRVEGIVSLRDIFKFLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A452</accession>
    <name>A0AA39A452_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_006098</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A452"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.330:FF:000005">
        <property type="entry name" value="1-aminocyclopropane-1-carboxylate oxidase homolog 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10209:SF791">
        <property type="entry name" value="1-AMINOCYCLOPROPANE-1-CARBOXYLATE OXIDASE HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10209">
        <property type="entry name" value="OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="7">
        <location>
            <begin position="216"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9700626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="367" mass="41604" checksum="991EFDFF2154F853" modified="2024-01-24" version="1">MVVTSFSESPMATQADYDRLSELKAFDETKAGVKGLVDAGVSQVPRIFIQPPDDFTTGDTEFSFPVIDLQDMNTDPDRRKEIVDMVRDASETWGFFNVVNHGISVTVLEEMKNGVRRFYEQDTEVKKQYYSRDLERKVVYNSNFDLYKAPAANWRDTFYFLMAPQPPDPQELPPAFRDILIEYKDEVMKLGFKLLELISEALGLKANHLKDMDCAEGLAMLCHYYPACPQPELTMGTTKHADNDFLTVLLQDEIGGLQVLHQDQWVDVSPMPGALVINIGDLLQLITNDRFKSVEHRVLASHKGPRVSVACFFSTALLPSLKLYGPIKELLSEENPPKYREITVRDFVAYFNAKGLDGTSALEHFKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A591</accession>
    <name>A0AA39A591_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycosyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_006527</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="5"/>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A591"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080043">
        <property type="term" value="F:quercetin 3-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080044">
        <property type="term" value="F:quercetin 7-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000167">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000237">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926">
        <property type="entry name" value="GLUCOSYL/GLUCURONOSYL TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926:SF870">
        <property type="entry name" value="UDP-GLYCOSYLTRANSFERASE 75B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9701220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="469" mass="51967" checksum="07B69C3653C22207" modified="2024-01-24" version="1">MGSPHFLLVTFPAQGHINPALQFAKRIIRTGAQVSFATSVSAHRRMPKRPTPEGLNFVPFSDGYDDGFKPADDAQHYMSEIKRRGSETLREIIVRNADEGRPFTCIVYTLLLPWAAEVARGLGVPSALLWIQPATVLDIYYYYFNGYGDAFRNISNEPSCSVELPGLPPLSSRDLPSFLVKSNAYTFVLPTFQEQLEALSQETSPKVLVNTFDALEPEPLRAVDKLHLIGIGPLVPSAYLDGKDPSDTSFGGDMFQGSDDYMEWLNSKPKSSVVYVSFGSLSVLSKTQKEEIARALLDCGHPFLWVIRAPENGEEVKEEDKLSCREELEQKGLIVSWCSQIEVLTHPSLGCFVSHCGWNSTLESLVSGVPVVAFPQWTDQGTNAKLIEDMWKIGIRVTVNEEGIVESDEFKRCLEIVMGGGEKGEEMRRNAEKWKNLAREAVKDGGSSDKNLKGFVDEVGHGCYESIGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A5C5</accession>
    <name>A0AA39A5C5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_005854</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A5C5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010052">
        <property type="term" value="P:guard cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000064">
        <property type="entry name" value="Transcription factor MUTE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044283">
        <property type="entry name" value="FAMA/SPEECHLESS/MUTE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46684">
        <property type="entry name" value="TRANSCRIPTION FACTOR FAMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46684:SF1">
        <property type="entry name" value="TRANSCRIPTION FACTOR MUTE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="7">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9700228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="190" mass="21331" checksum="B4457E60C6130C48" modified="2024-01-24" version="1">MSHIAVERNRRRQMNEHLKVLRSLTPCFYIKRGDQASIIGGVIEFIKELHQVLQSLESKKQRKSLSPSPGPSPRPLQLTSQPDTPFGLENFKELGACCNSSVADVEAKISGSNVILRIISRRIPGQIVKIINVLEKLSFEVLHLNISSMEETVLYSFVIKIGLECQLSVEELALEVQQSFRSDAVYANEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A7Q6</accession>
    <name>A0AA39A7Q6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Nitrate regulatory gene2 protein-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_004239</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A7Q6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006868">
        <property type="entry name" value="DUF630"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006867">
        <property type="entry name" value="DUF632"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21450:SF7">
        <property type="entry name" value="DNA LIGASE (DUF630 AND DUF632)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21450">
        <property type="entry name" value="PROTEIN ALTERED PHOSPHATE STARVATION RESPONSE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04783">
        <property type="entry name" value="DUF630"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04782">
        <property type="entry name" value="DUF632"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF630" evidence="3">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="DUF632" evidence="2">
        <location>
            <begin position="267"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="205"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9702406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="705" mass="79981" checksum="B3F3113BAB5895A0" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AG45</accession>
    <name>A0AA39AG45_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_002120</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AG45"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050231">
        <property type="entry name" value="Iron_ascorbate_oxido_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47990">
        <property type="entry name" value="2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0408">Iron</keyword>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="4">
        <location>
            <begin position="204"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9706998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="357" mass="40138" checksum="BDA256D06AFD5CAD" modified="2024-01-24" version="1">MALEIEEHPVESLSSGPKLCVKNFVWSEDEWPLINHDDFADGDDIPTISLEGNLSGKPCQDYDKVCQAMVTACEKWGFFKLVDHGVAIETVENVKVQLNELFDLPMDQKLKGARSTSLPLGYCASNPEYGQNLPWAEILQLLQSPQQVVAFARKVFGDQHQPFSNAMVKYMQALDKLGMKIFEMLAHGMGLPDDFFTKNFEEKEATMIRVNRYPPCPLPEKCLGVGSHSDPHTLTILLQDDVGGLQVLKSDNQWIGIRPVPNSFVINIGDTLEAWTNGRLRSVVHRAVVNKEKHRLSVAYFLSPASSAIIDCPPQLIESSTNLRKYVSFTWGEFRKELLTQKRVVGKTALNRYLMSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AG60</accession>
    <name>A0AA39AG60_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein EXECUTER 1, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_003633</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AG60"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042651">
        <property type="term" value="C:thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010343">
        <property type="term" value="P:singlet oxygen-mediated programmed cell death"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044680">
        <property type="entry name" value="EX1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33917">
        <property type="entry name" value="PROTEIN EXECUTER 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33917:SF3">
        <property type="entry name" value="PROTEIN EXECUTER 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12014">
        <property type="entry name" value="Cyclin_D1_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="293"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="469"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="293"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="470"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9705659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="702" mass="78618" checksum="923D4BED400C6B3B" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AGC0</accession>
    <name>A0AA39AGC0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Probable purine permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_002210</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the purine permeases (TC 2.A.7.14) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AGC0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005345">
        <property type="term" value="F:purine nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015211">
        <property type="term" value="F:purine nucleoside transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030182">
        <property type="entry name" value="PUP_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31376">
        <property type="entry name" value="OS09G0467300 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31376:SF16">
        <property type="entry name" value="PURINE PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16913">
        <property type="entry name" value="PUNUT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="43"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="144"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="174"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="207"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="249"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="288"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="316"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="344"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9707115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="388" mass="42864" checksum="2E8F48A99FFC16F9" modified="2024-01-24" version="1">MEAAQELQLQLQTRAESREGSSLRDATVSGNQPSLPKPILYKWWLRVAIYTLFLLAGQSTATLLGRLYYDKGGNSKWMATFVQSAGFPILLPLFFFFSPSSKSNPISISPSSAKPPSFSTILFLYLFFGLLLTGDNLMYSYGLLYLPVSTYSLLCATQLAFNGLFSFFLNSQKFTMLILNSLVLLTISASLLAVHSDSEDTTGTPKGKYVIGFLCTLGASATYSLYLSLVQLSFQKVIKRETFDVVLEMQVYPSFVATCACVVGLFASGEWSGLSEEMKEYEDGKISYLMTLIWTAVTWQISSVGLLGLIFEVSSLFSNVISTVALPVVPVVAVMFFHDKMDGVKVMALLLAVWGFVSYIYQHYLDDCRSKKTKTNEGEASNALTEVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AJH2</accession>
    <name>A0AA39AJH2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_000598</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AJH2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044550">
        <property type="term" value="P:secondary metabolite biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19124">
        <property type="entry name" value="AKR_AKR4A_4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000014">
        <property type="entry name" value="NAD(P)-linked oxidoreductase superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044497">
        <property type="entry name" value="AKR4A/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11732">
        <property type="entry name" value="ALDO/KETO REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00798">
        <property type="entry name" value="ALDOKETO_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00063">
        <property type="entry name" value="ALDOKETO_REDUCTASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="5">
        <location>
            <begin position="19"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="4">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9708646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="320" mass="35566" checksum="AE4468F9406F4BC4" modified="2024-01-24" version="1">MADIPGKPVGSGHARIPLLGFGTAVFPFTASETMKESILHAIKLGYRHIDAAALYNSEQPVGEAIAEALGLGLIKSRAELFITSKLWCTDAHPDLVLPALRRTLKNLGLEYLDLYLIHFPVSLKPGSCEVPFEEKDLLPLDFKSVWEAMEESQSLGLSKLIGVSNFSCKKLQDLLVTAKIPPAVNQVEMNPLWQQKKLREFCNGKGIHVTAYSPLGGRGTLWGTNRVLDCKVLQGIAQAKGKTVAQICLRWALEQGVSILVKSFNKERIEENLEILDWKLSPEESQKIDNIEQQRGFLAPMFVSEHGPYKSLEDFWDGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AKJ4</accession>
    <name>A0AA39AKJ4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_000986</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AKJ4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001678">
        <property type="entry name" value="Calmodulin-binding receptor kinase CaMRLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000111">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046956">
        <property type="entry name" value="RLP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063">
        <property type="entry name" value="LRR RECEPTOR-LIKE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063:SF100">
        <property type="entry name" value="RECEPTOR-LIKE PROTEIN EIX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041360865" description="Leucine-rich repeat-containing N-terminal plant-type domain-containing protein" evidence="13">
        <location>
            <begin position="22"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="872"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="14">
        <location>
            <begin position="38"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="15">
        <location>
            <begin position="228"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9709292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="916" mass="102141" checksum="A00FAE694A309833" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AKL9</accession>
    <name>A0AA39AKL9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein CPR-5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_001056</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AKL9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010150">
        <property type="term" value="P:leaf senescence"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010090">
        <property type="term" value="P:trichome morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044708">
        <property type="entry name" value="CPR5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35322">
        <property type="entry name" value="PROTEIN CPR-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35322:SF2">
        <property type="entry name" value="PROTEIN CPR-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="233"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="264"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="288"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="330"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9709398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="372" mass="40953" checksum="D59A5360E277B3E1" modified="2024-01-24" version="1">MTPKRSLLNRLIENAVNELKKQRNVEAGGCLGHFNTQNSGSDLNLPVPLNSVDTTISSSISDHYSSMGFHSVATLECLNTLEERQKSMQTDSANRELTIHGQISEQLACVSSSSLGSVMNQSMLSTSEKSIVEQACCNDLKTPELILSMKKMKLKETQLALNSNSYLLGRMKFSMEDTRYAGLLRKCIDCLVAGLLIMSGSRVWSMISSLGGSRKPMASFNPGFYMLRCQVQVARQMMFGIFMIAAIAYLLLQRSASSKQPTSVTFIILLLGVICVAGKFRVDTLGGTSMLTAFLFKYIYLGFVSSPWSPITVSQGAKGNTMIPYWIRRILIYAAIFLLLPLLCGLMPFASPSEWTDHFLLLIADPLSNVGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AL71</accession>
    <name>A0AA39AL71_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Metallo-beta-lactamase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_000333</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AL71"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009536">
        <property type="term" value="C:plastid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000534">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.15.10:FF:000032">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050662">
        <property type="entry name" value="Sec-metab_biosynth-thioest"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23131">
        <property type="entry name" value="ENDORIBONUCLEASE LACTB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23131:SF0">
        <property type="entry name" value="ENDORIBONUCLEASE LACTB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="501"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="2">
        <location>
            <begin position="226"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9708224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="529" mass="59173" checksum="83CD548D6C76B386" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ALY8</accession>
    <name>A0AA39ALY8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9">DNA-directed RNA polymerase</fullName>
            <ecNumber evidence="2 9">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_001099</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the phage and mitochondrial RNA polymerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="2 9"/>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ALY8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034245">
        <property type="term" value="C:mitochondrial DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006390">
        <property type="term" value="P:mitochondrial transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1320.10:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000027">
        <property type="entry name" value="DNA-directed RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.260:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.280:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1320.10">
        <property type="entry name" value="DNA-directed RNA polymerase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024075">
        <property type="entry name" value="DNA-dir_RNA_pol_helix_hairp_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046950">
        <property type="entry name" value="DNA-dir_Rpol_C_phage-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002092">
        <property type="entry name" value="DNA-dir_Rpol_phage-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037159">
        <property type="entry name" value="RNA_POL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029262">
        <property type="entry name" value="RPOL_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10102">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10102:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00940">
        <property type="entry name" value="RNA_pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14700">
        <property type="entry name" value="RPOL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01311">
        <property type="entry name" value="RPOL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00900">
        <property type="entry name" value="RNA_POL_PHAGE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00489">
        <property type="entry name" value="RNA_POL_PHAGE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5 9" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase N-terminal" evidence="11">
        <location>
            <begin position="145"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="269"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="276"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9709460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="978" mass="110681" checksum="EFE985CD5F6BF882" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ARS9</accession>
    <name>A0AA39ARS9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Polysaccharide biosynthesis domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_001370</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ARS9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045492">
        <property type="term" value="P:xylan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006514">
        <property type="entry name" value="IRX15/GXM/AGM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01627">
        <property type="entry name" value="A_thal_3515"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31444">
        <property type="entry name" value="OS11G0490100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21729">
        <property type="entry name" value="IRX15_IRX15L_GXM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9709859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="283" mass="31868" checksum="7D4AEC61AC22E15E" modified="2024-01-24" version="1">MMKKRHLLEKPWFLRLALVGLIAGVLFIYSFVLNSDHSLLCGDSPRADSSAAQIQLSAILHYATSRIVPQQTLTEITVSFDVLQSLASCNFLVFGLGFDSLMWSSLNARGDTLFLEEDPKWVQTVLKDAPTLRVHTVRYRTHLSEADDLLSTYGSVPECKPPKAYLRGNNRCKLALSDLPDEVYDKEWDLIMIDAPRGYFLEAPGRMAAIFSAAVMARARVRPGVTHVFLHDVDRRVEKVYAEEFLCRKYLVGSVGRLWHFEIPPTANLSRASDSTGFCKNEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D4Q3</accession>
    <name>A0AA39D4Q3_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_027157</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9671036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D4Q3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9671036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="95" mass="10512" checksum="DAF498E41F8A186A" modified="2024-01-24" version="1">MGIAEKKMHKQGLCGSYPYCHNTCAGPAQLYHFPLIQLRYQVCHEGLQTCSITQCPNANVLEDHACISSNTASEQVQEAQASCQHRQCSLQNSTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D6E4</accession>
    <name>A0AA39D6E4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_023289</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D6E4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9673643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="120" mass="13863" checksum="D066C0E954B69F38" modified="2024-01-24" version="1">MGLWHRLFQLAKLDWVPPRSISEMMLINFKGFGKSKRGLILWQNACIALIWVVWRERNARIFEDKARSSENLWDSIHFLTSLWAFCSAVFKGIPLNVLQLDWLAVCSPNGMVESRALALS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D6Y5</accession>
    <name>A0AA39D6Y5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NADH-ubiquinone oxidoreductase 21kDa subunit N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_025790</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D6Y5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053229">
        <property type="entry name" value="NADH-Q_oxidrdct_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019721">
        <property type="entry name" value="NADH-UbQ_OxRdtase_su21_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34062:SF1">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 21KDA SUBUNIT N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34062">
        <property type="entry name" value="OXIDOREDUCTASE 21 KDA SUBUNIT, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G04750)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10785">
        <property type="entry name" value="NADH-u_ox-rdase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="NADH-ubiquinone oxidoreductase 21kDa subunit N-terminal" evidence="2">
        <location>
            <begin position="11"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9672310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="100" mass="10913" checksum="D94B24DE68503CAB" modified="2024-01-24" version="1">MNTDITASEKPQYPVIDRNPPLTKVVGNFSALDYLRFVTITGVSVTVGYLSGIKPNIRGPSMVTGGLIGLMGGFMYAYQNSAGRLMGFFPNEDEVARYKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D8G2</accession>
    <name>A0AA39D8G2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_023853</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D8G2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010158">
        <property type="term" value="P:abaxial cell fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000002">
        <property type="entry name" value="Myb family transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044847">
        <property type="entry name" value="KAN_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006447">
        <property type="entry name" value="Myb_dom_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01557">
        <property type="entry name" value="myb_SHAQKYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31496">
        <property type="entry name" value="TRANSCRIPTION FACTOR KAN2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31496:SF25">
        <property type="entry name" value="TRANSCRIPTION FACTOR KAN3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0221">Differentiation</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="138"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="224"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="231"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9674559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="337" mass="36980" checksum="21639EA9263E7E68" modified="2024-01-24" version="1">MFFSSNPIMGSTSPLPDLSLQISPPSISDCEVKEVGYDGFMKKSFYSDRSSATDSGSSGSDLSHENGFFSPESGFNLGPVEPTLSLGLEMAALIPPPLELPRNLHHNHHNHQPQIYGREFKRNSRMMNGSKRSIRAPRMRWTTTLHAHFVHAVQLLGGHERATPKSVLELMNVKDLTLAHVKSHLQMYRTVKSTDKGTGQGQTDMGLNQRTGIGQVELGGLSCDKADAPPSFSSNTPQPSTPQNISRSSWSLSMETNDEGRFSHENGLKYPHLMTNDAKVEGHMVALHVAEGAKERVDSSALSPSDMLLNLEFTLGRPSLQIDYADQSPNELALLKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D8G5</accession>
    <name>A0AA39D8G5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Prohibitin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_024595</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Component of a prohibitin multimeric complex in mitochondrial membranes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the prohibitin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D8G5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03401">
        <property type="entry name" value="SPFH_prohibitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000163">
        <property type="entry name" value="Prohibitin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23222">
        <property type="entry name" value="PROHIBITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23222:SF1">
        <property type="entry name" value="PROHIBITIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00679">
        <property type="entry name" value="PROHIBITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="Band 7/SPFH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041204395" description="Prohibitin" evidence="8">
        <location>
            <begin position="21"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="Band 7" evidence="9">
        <location>
            <begin position="5"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9675743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="173" mass="19397" checksum="3756800F53F2FDA9" modified="2024-01-24" version="1">MRVHNSLLVFLVGELGLYEAINSLYNVEGGHQAIVFNGIIVIYDVRARPHLVESTSGSCDLQMVKIGLQVLTRPVPDQLPAIYQTLGENYNERVLPSIIHETLKAVVITQREAISREIRKILTEREANFNIALDDVSITSLTFGKEFIATIEAKQATIWKVFEGCYLLEVPSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D9R0</accession>
    <name>A0AA39D9R0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Subtilisin-like protease SBT1.9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_023745</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the peptidase S8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D9R0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02120">
        <property type="entry name" value="PA_subtilisin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04852">
        <property type="entry name" value="Peptidases_S8_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000006">
        <property type="entry name" value="Subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.80:FF:000003">
        <property type="entry name" value="Subtilisin-like protease SBT1.9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034197">
        <property type="entry name" value="Peptidases_S8_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010259">
        <property type="entry name" value="S8pro/Inhibitor_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045051">
        <property type="entry name" value="SBT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041469">
        <property type="entry name" value="Subtilisin-like_FN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10795">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17766">
        <property type="entry name" value="fn3_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05922">
        <property type="entry name" value="Inhibitor_I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 10" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 10" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041250497" description="Subtilisin-like protease SBT1.9" evidence="11">
        <location>
            <begin position="30"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="domain" description="Inhibitor I9" evidence="13">
        <location>
            <begin position="33"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="12">
        <location>
            <begin position="143"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="domain" description="Subtilisin-like protease fibronectin type-III" evidence="14">
        <location>
            <begin position="655"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="9 10">
        <location>
            <position position="152"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="9 10">
        <location>
            <position position="223"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="9 10">
        <location>
            <position position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF05922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF17766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9674377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="763" mass="81815" checksum="D265811FF4E05ADC" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DA96</accession>
    <name>A0AA39DA96_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase</fullName>
            <ecNumber evidence="3">3.2.2.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_024815</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>NAD(+) + H2O = ADP-D-ribose + nicotinamide + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16301"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57967"/>
            <dbReference type="EC" id="3.2.2.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:16302"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the disease resistance TIR-NB-LRR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.6" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9676015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061809">
        <property type="term" value="F:NAD+ nucleosidase activity, cyclic ADP-ribose generating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050832">
        <property type="term" value="P:defense response to fungus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043068">
        <property type="term" value="P:positive regulation of programmed cell death"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.430:FF:000002">
        <property type="entry name" value="Disease resistance protein (TIR-NBS-LRR class)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10140:FF:000007">
        <property type="entry name" value="Disease resistance protein (TIR-NBS-LRR class)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000386">
        <property type="entry name" value="Disease resistance protein RPS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045344">
        <property type="entry name" value="C-JID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058546">
        <property type="entry name" value="RPS4B/Roq1-like_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058192">
        <property type="entry name" value="WHD_ROQ1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017:SF570">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN (TIR-NBS CLASS)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20160">
        <property type="entry name" value="C-JID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23286">
        <property type="entry name" value="LRR_13"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23282">
        <property type="entry name" value="WHD_ROQ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0611">Plant defense</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="TIR" evidence="14">
        <location>
            <begin position="18"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1407"/>
            <end position="1427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1418"/>
            <end position="1427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00061488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9676015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1441" mass="164277" checksum="64902CBC9BB95C61" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DCY8</accession>
    <name>A0AA39DCY8_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_022596</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DCY8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47481">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47481:SF22">
        <property type="entry name" value="RETROTRANSPOSON GAG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9677707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="193" mass="22404" checksum="7BFFF3417738E5CD" modified="2024-01-24" version="1">MRLRLPRLPAMEKMHHSKCPRVLHLCCYEESFQMNAYLKFTSCGLEEDNLLCLATIWSVDPNIVPLVTGTKSFIEAWSCLERTDANASRSHKLNLKTTLARINMDTMSVNDYLLTIKHMANDLALIGALVSEDELLLHVLNDLNLEYKKLGVVMCAKENPISFKKLHHKLIHHEIYLKRNKERKESFRCYFTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DD46</accession>
    <name>A0AA39DD46_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_021555</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DD46"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9679661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="238" mass="26898" checksum="B1219FDCCC20C986" modified="2024-01-24" version="1">MMILSSLRIPKRTRVPFMMSFLLGWKSCLVLKSVETGVVDSLVVPVPKSGDSQETGLPDSEDLNESSSNVEHKTEPHSDFPHVEDLREKKVKNQFPAKDIADLTPEKRTVPIQEKDLPPYPHISTTSSDSGTSCEGNTDMKDQEAIPKAPLSYHVKYCMQDWWVSITAFFGSLQFQKMRRMTEKPFNWMIGMMKTTTFWDDTSALSFSSGDLHAQPAAPLPITRAMQRTTRVSFQSLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DDA1</accession>
    <name>A0AA39DDA1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Kinesin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_021407</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. KIN-10 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DDA1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005875">
        <property type="term" value="C:microtubule associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051231">
        <property type="term" value="P:spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.280:FF:000003">
        <property type="entry name" value="Kinesin-like protein KIN-10C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000098">
        <property type="entry name" value="Kinesin-like protein KIN-10C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.280">
        <property type="entry name" value="AF1531-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969:SF9">
        <property type="entry name" value="KINESIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="RuvA domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0493">Microtubule</keyword>
    <keyword evidence="2 4" id="KW-0505">Motor protein</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="6">
        <location>
            <begin position="21"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="356"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="371"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="114"/>
            <end position="121"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00061615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9679465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="678" mass="74664" checksum="B73F713CA351D5CD" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DFW7</accession>
    <name>A0AA39DFW7_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_018445</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DFW7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9682526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="89" mass="9989" checksum="FD758F8D24F95532" modified="2024-01-24" version="1">MGVEGTLEYLSNLMSSGHKHKKRKQLQTVELKIRMDWEGCEFKVKKTLSSFSGQKATVTGYANANKVSKQAIQKESAEKTARKAEREGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DFX0</accession>
    <name>A0AA39DFX0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Scarecrow-like protein 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_019888</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the GRAS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DFX0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005202">
        <property type="entry name" value="TF_GRAS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31636">
        <property type="entry name" value="OSJNBA0084A10.13 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03514">
        <property type="entry name" value="GRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50985">
        <property type="entry name" value="GRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="29"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="VHIID" evidence="3">
        <location>
            <begin position="470"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Leucine repeat II (LRII)" evidence="3">
        <location>
            <begin position="549"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="SAW" evidence="3">
        <location>
            <begin position="678"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="VHIID" evidence="3">
        <location>
            <begin position="501"/>
            <end position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9680707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="750" mass="81960" checksum="08F8DAF64A8A07B5" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DGR1</accession>
    <name>A0AA39DGR1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_020444</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9681577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DGR1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46567">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46567:SF1">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="18"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="470"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="658"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="663"/>
            <end position="675"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9681577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="751" mass="82483" checksum="F400D955A8BF50E9" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DHN9</accession>
    <name>A0AA39DHN9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_016810</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DHN9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000440">
        <property type="entry name" value="Putative RNA-binding protein ARP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11176">
        <property type="entry name" value="BOULE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11176:SF56">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="16"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="95"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="101"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9684521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="256" mass="27449" checksum="73B7F7D53882A3E8" modified="2024-01-24" version="1">MTMSNAIGQFGDTTLTKVFVGGLAWETPKEAMRDHFEKYGEILEAVIISDKLTGRSKGYGFVTFKEPEAAKKACEDATPMINGRRANCNLASLGARRPRSAASSTPPHQGSNGGSRPTAPAPAPANHVQWYYPAGTPAPPFHHQHQQAVPFYGYSPAYIAADISYNHKLSYTGGSYMNGHFSQVYPGQAMVGANAMMPMYPFYHFHQSQTMGHPAHMFPPTTVGPMPTVPAIISKPASMAPNTGAIGGGESFKKVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DIM1</accession>
    <name>A0AA39DIM1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Agamous-like MADS-box protein AGL15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_017563</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DIM1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00265">
        <property type="entry name" value="MADS_MEF2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050142">
        <property type="entry name" value="MADS-box/MEF2_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033896">
        <property type="entry name" value="MEF2-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002487">
        <property type="entry name" value="TF_Kbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48019">
        <property type="entry name" value="SERUM RESPONSE FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01486">
        <property type="entry name" value="K-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00404">
        <property type="entry name" value="MADSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51297">
        <property type="entry name" value="K_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00350">
        <property type="entry name" value="MADS_BOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="8">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="K-box" evidence="9">
        <location>
            <begin position="83"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="225"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="117"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9685571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="247" mass="28058" checksum="D420865DC1F03F07" modified="2024-01-24" version="1">MGRGKIEIKKIENANSRQVTFSKRRVGLLKKASELAILCDAQVGVIIFSNTGKLFEFSSTSMKRIISRYNKLDSSEGALVEYKAEEPKEADILKDEIQKLQTKQLQLLGKDLSGLSLKELQNLEQQLNESLLSVKERKEQVLMEQLEQSRVQEQRAILENETLRRQVEELRGLVPSSDRLVPPFLEYHPLERKDSITKSVVISPDVCDFAVEREESDTTLQLGLPTEISRKRKAPAEMETRSNNSGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DIX1</accession>
    <name>A0AA39DIX1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Import inner membrane translocase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_016319</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9683767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DIX1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36354">
        <property type="entry name" value="IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36354:SF2">
        <property type="entry name" value="IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="153"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="350"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="350"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="360"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9683767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="382" mass="42529" checksum="F290CBF8E87B1475" modified="2024-01-24" version="1">MAKPPQRLFQHHSQFLGLLRSHINRLHFHDKPSISLPAAGSLSPSSAIRSPDPLSTTLSFSSRVESFQIQSKRLSKPCPIPPIFSSGFGARLYANSSGLRYFSSGGWNLGKAQTNANFPKAFLDLPLRSLRSAFYRYREAVGLQIEAFWKRNYVFLLGAGGVVLCAVLWRVMFGVATTFVGLSEGMAKYGFLALSASIVAFSGLYIRSRLTINPDKVYRIAMRRLNTSAGILEVMGAPLTGTDLRAYVMSGGGLSLKKFKPTLRSKRCFLIFPIRGSERRGLVSIEVKKKKGEYDMKLLAVDIPMATGPDQRLFLIGDEEEYKVGGGLISELRDPVVKAMAATKEFEELDQIEEEEDAERELQEAERKHREEIEKLEKGAPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DJ66</accession>
    <name>A0AA39DJ66_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PVL29_015211</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DJ66"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015369">
        <property type="term" value="F:calcium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.30:FF:000019">
        <property type="entry name" value="Sodium/calcium exchanger NCL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004713">
        <property type="entry name" value="CaH_exchang"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31503:SF45">
        <property type="entry name" value="SODIUM_CALCIUM EXCHANGER NCL-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31503">
        <property type="entry name" value="VACUOLAR CALCIUM ION TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0050">Antiport</keyword>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="15 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="18" id="KW-0732">Signal</keyword>
    <keyword evidence="13" id="KW-0915">Sodium</keyword>
    <keyword evidence="16" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="12" id="KW-0346">Stress response</keyword>
    <keyword evidence="7 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041284446" description="EF-hand domain-containing protein" evidence="18">
        <location>
            <begin position="25"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="64"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="141"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="202"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="229"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="425"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="456"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="496"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="524"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="550"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="19">
        <location>
            <begin position="293"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="19">
        <location>
            <begin position="333"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAJ9686201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="577" mass="62820" checksum="53B33206876E6EF9" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DJ83</accession>
    <name>A0AA39DJ83_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Reticulon-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_015254</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Endoplasmic reticulum membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DJ83"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003388">
        <property type="entry name" value="Reticulon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044647">
        <property type="entry name" value="RTNLB17/18/21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46626">
        <property type="entry name" value="RETICULON-LIKE PROTEIN B17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46626:SF2">
        <property type="entry name" value="RETICULON-LIKE PROTEIN B17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02453">
        <property type="entry name" value="Reticulon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50845">
        <property type="entry name" value="RETICULON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="224"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="Reticulon" evidence="8">
        <location>
            <begin position="185"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="110"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="397"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="39"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="121"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="141"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="156"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="420"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9686261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="430" mass="48012" checksum="32D3709BFC24D3DF" modified="2024-01-24" version="1">MDSPPLSHQSEPRSGTKSASRLARLGEIDQGGQERPNISLDLSKLSPSKTLSPSNPLPLHEILLLSPSPLRRSRTRLTERIDMGEEPLEATGSRRRCKSRISQMGVLGCASPRNARRPRRRVEQDVREDKDFGMAEEMGKLRKRRHSGRSRKEKLSLVPSVPSSSSSPKAIEDDLSNLYRIGQLVTDLIMWKDASKSSFWFGFGSVCFLSSCFTRGISFSIFSAISQLGLLFLGLSFFSKSLYQRDNVEKRREFKLNEDDILRVARAILPATNLAISKTRQLFSGEPSMTLKVAPFFLVGAEYGHLLTVRRLCALGFFISFTIPKLYSCYSAQINTRVEYLKGWVVEAWGACSHKKIVAASAVTVFWNLSAIKTRIFAAFISLVVLRYHRQQSEANIEEREADGEEKEQQQQPQLTMVVSGGGGGGGSQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DR34</accession>
    <name>A0AA39DR34_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ABC-type xenobiotic transporter</fullName>
            <ecNumber evidence="3">7.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_013349</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O + xenobioticSide 1 = ADP + phosphate + xenobioticSide 2.</text>
            <dbReference type="EC" id="7.6.2.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.2" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902417">
        <property type="term" value="F:(+)-abscisic acid D-glucopyranosyl ester transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008559">
        <property type="term" value="F:ABC-type xenobiotic transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18579">
        <property type="entry name" value="ABC_6TM_ABCC_D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18580">
        <property type="entry name" value="ABC_6TM_ABCC_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000013">
        <property type="entry name" value="ABC transporter C family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000024">
        <property type="entry name" value="ABC transporter C family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000450">
        <property type="entry name" value="ABC transporter C family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000163">
        <property type="entry name" value="Multidrug resistance-associated protein member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044746">
        <property type="entry name" value="ABCC_6TM_D1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044726">
        <property type="entry name" value="ABCC_6TM_D2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF456">
        <property type="entry name" value="MULTIDRUG RESISTANCE-ASSOCIATED PROTEIN LETHAL(2)03659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="37"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="76"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="112"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="143"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="174"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="338"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="441"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="519"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="557"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="911"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="958"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1028"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1055"/>
            <end position="1074"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1140"/>
            <end position="1160"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="16">
        <location>
            <begin position="304"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="15">
        <location>
            <begin position="615"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="16">
        <location>
            <begin position="920"/>
            <end position="1202"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="15">
        <location>
            <begin position="1239"/>
            <end position="1473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="852"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="873"/>
            <end position="882"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9691132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1623" mass="182279" checksum="69E1A583B7C7B0DC" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DRH9</accession>
    <name>A0AA39DRH9_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_011952</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Endomembrane system</location>
            <topology evidence="11">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9693040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DRH9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000111">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051502">
        <property type="entry name" value="RLP_Defense_Trigger"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48062">
        <property type="entry name" value="RECEPTOR-LIKE PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48062:SF52">
        <property type="entry name" value="RECEPTOR-LIKE PROTEIN 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="144"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9693040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="203" mass="23193" checksum="E8E6C91D9D0F1751" modified="2024-01-24" version="1">MSGLDLSCNNLTGEIPHELGMLSSILALNLSHNQLKGPIPKSFSNLSQIESLDLSYNKLSGEIPPEFIELNFLEVFSVAHNNISGRVPDMKAQFGTFGESSYEDNPFLCGLMLKRICNTSIEPPYSPPKSSKESETKWYDIDRVVFFASFAASYMMILLGIVTILYINPYWRQRWFNFIEECIYSCYYFASDTYSKLLAYLYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DS47</accession>
    <name>A0AA39DS47_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GDSL esterase/lipase APG</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_011332</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DS47"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1110:FF:000003">
        <property type="entry name" value="GDSL esterase/lipase APG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050592">
        <property type="entry name" value="GDSL_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45642">
        <property type="entry name" value="GDSL ESTERASE/LIPASE EXL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45642:SF35">
        <property type="entry name" value="GDSL ESTERASE_LIPASE APG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041324308" description="GDSL esterase/lipase APG" evidence="2">
        <location>
            <begin position="25"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9692212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="357" mass="38808" checksum="CA693480CCB41E3E" modified="2024-01-24" version="1">MAIWGGAAALVLALALQFATMADAQGTTSVVPAMILFGDSAVDIGNNNYFPTAFKANYLPYGKDFISHRPTGRFCNGKLATDITADILGFETYPPAYLSPQATGKNLLIGANFGSAAAGYDDNTAIINHAIPLSQQLEYYKEYRVKLAKVAGSKRAAAILKGALYLVGFGTADFLQNYYVNPSLKKLYTPDQYSTYLATIFSSFIKDLYGLGARKIGVVPLPPLGCFPETITLFRFRKHGCIARINKNAKGFNNKINTTAISLQKQLPALKIVVFDIFKPLHDVFTSPSDYGFAEARKGCCQTRKTGTGTAPILCDPKSPGTCWNASQYVFWDDVHLSQATNRILAESMLLQGISLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DTB2</accession>
    <name>A0AA39DTB2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">3'-5' exonuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_008759</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DTB2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06141">
        <property type="entry name" value="WRN_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000054">
        <property type="entry name" value="Werner Syndrome-like exonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002562">
        <property type="entry name" value="3'-5'_exonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051132">
        <property type="entry name" value="3-5_Exonuclease_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13620">
        <property type="entry name" value="3-5 EXONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13620:SF121">
        <property type="entry name" value="EMB|CAB82946.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01612">
        <property type="entry name" value="DNA_pol_A_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00474">
        <property type="entry name" value="35EXOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3'-5' exonuclease" evidence="3">
        <location>
            <begin position="20"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9696701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="196" mass="22456" checksum="18BB3EF9B1E221B6" modified="2024-01-24" version="1">MSYTHLVSFDNKAIETTVTDRGSEADSWVNTILAVYRGGDMIVGLGCEWIPTFLSRTSDRIATLQLCVDTKCLILQLFYMDYIPQSFKNFLSNPAVTFVGVEVESDAMKLRDEYELDCQKTSNIRALACSFWPNRWYRRPGLKDLAFQIVGLLMQKPIHVCSSNWEARILSNEQVEYASIDAYAAYRIGHRLLKEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DU85</accession>
    <name>A0AA39DU85_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_010037</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DU85"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033249">
        <property type="entry name" value="CLE_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34545">
        <property type="entry name" value="CLAVATA3/ESR (CLE)-RELATED PROTEIN 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34545:SF8">
        <property type="entry name" value="CLAVATA3_ESR (CLE)-RELATED PROTEIN 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041352803" evidence="2">
        <location>
            <begin position="36"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9694342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="98" mass="10557" checksum="2647F1D84EF0B990" modified="2024-01-24" version="1">MIWGLSKKQMGVRKELACLSLLLLMLLQLESSCFAEGYGRFSRFKVGSGSGSGSGSGSELSPAKTRGVFKGNADKDGDDVFAAEKRRVYTGPNPLHNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DXZ3</accession>
    <name>A0AA39DXZ3_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_008250</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9699565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DXZ3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000597">
        <property type="entry name" value="Ribosomal_uL3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00297">
        <property type="entry name" value="Ribosomal_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9699565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="35" mass="3895" checksum="280577D606650BB0" modified="2024-01-24" version="1">MKIKVVTIIRTPPMVIVGVVNHVKTLRGLHSLNMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DZS4</accession>
    <name>A0AA39DZS4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NB-ARC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_004545</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DZS4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050905">
        <property type="entry name" value="Plant_NBS-LRR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463:SF198">
        <property type="entry name" value="RPP4C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0611">Plant defense</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9702848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="136" mass="15251" checksum="91781BF1FECDF6E1" modified="2024-01-24" version="1">MTSDSIEKHDLKPTTEKMLKKCRGLPIAIVIVAKALNGKDSIAWKDVVRQLTQSIETTVKGIEAKIFLTLELSYNSLYSNEVKSFFLLCSLLPYGATPIDNLFKYGVGLDLFQNINSLEEAWDRTFLLWSCGAVCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E0U8</accession>
    <name>A0AA39E0U8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_006372</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Required for vesicular transport from the ER to the Golgi complex. Functions as a SNARE associated with ER-derived vesicles.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the BET1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E0U8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15853">
        <property type="entry name" value="SNARE_Bet1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000033">
        <property type="entry name" value="bet1-like SNARE 1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039899">
        <property type="entry name" value="BET1_SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12791">
        <property type="entry name" value="GOLGI SNARE BET1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="104"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="14">
        <location>
            <begin position="33"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00060029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9701002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="123" mass="13936" checksum="2361757FDEE49CD7" modified="2024-01-24" version="1">MNSNLGDHRGNRAATFDGIEEGGIRASSSYSHEIDEVDNETAVDGLEDRVVMLKRLTGDIHEEVESHNRMLDRAGNEMDASRGILSGTMERFKMVFETKSSRRMFTLIASFVVIFLVIYYLTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E0Y8</accession>
    <name>A0AA39E0Y8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase</fullName>
            <ecNumber evidence="3">3.2.2.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_006459</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>NAD(+) + H2O = ADP-D-ribose + nicotinamide + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16301"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57967"/>
            <dbReference type="EC" id="3.2.2.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:16302"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the disease resistance TIR-NB-LRR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.6" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E0Y8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061809">
        <property type="term" value="F:NAD+ nucleosidase activity, cyclic ADP-ribose generating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050832">
        <property type="term" value="P:defense response to fungus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043068">
        <property type="term" value="P:positive regulation of programmed cell death"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10140:FF:000007">
        <property type="entry name" value="Disease resistance protein (TIR-NBS-LRR class)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000386">
        <property type="entry name" value="Disease resistance protein RPS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045344">
        <property type="entry name" value="C-JID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011713">
        <property type="entry name" value="Leu-rich_rpt_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058546">
        <property type="entry name" value="RPS4B/Roq1-like_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058192">
        <property type="entry name" value="WHD_ROQ1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017:SF570">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN (TIR-NBS CLASS)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20160">
        <property type="entry name" value="C-JID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23286">
        <property type="entry name" value="LRR_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07725">
        <property type="entry name" value="LRR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23282">
        <property type="entry name" value="WHD_ROQ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0611">Plant defense</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="TIR" evidence="14">
        <location>
            <begin position="14"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1173"/>
            <end position="1208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00061488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9701122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1208" mass="139152" checksum="379AB5487F2DACFA" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E124</accession>
    <name>A0AA39E124_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_006534</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E124"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080043">
        <property type="term" value="F:quercetin 3-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080044">
        <property type="term" value="F:quercetin 7-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000019">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000377">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926">
        <property type="entry name" value="GLUCOSYL/GLUCURONOSYL TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926:SF1534">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9701227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="447" mass="49765" checksum="EFE260BC52B3D838" modified="2024-01-24" version="1">MDKHHFLLLSYPAQGHINPTFHLAKLLLRLGVRVTFATFVSGFRRFSTLPTLPGLHFASVSDGYDDGNHSNYSMDETKRVGSQSLSNLLLSLSNECGPVTFLIYGLVVPWAATVAREHGIPSAFLSTQSATVIAVYHRYFKAHDGLFNTELGNPLNISLELPGLPPLKCEDLPSILLPTSPYACFIPSFQEHIQNLEQDPNPCVLVNTLDALEEDVIKALRDYMNVVAIGPLMQLDSSISCDLFERSKDYLPWMNSKPDRSVIYVSFGSLAVLQKKQMEEIFHGLMESHRPFLWVIRSTESEVEEMTNNSLSEEDGLIVQWCSQVEVLCHQAVGCFLTHCGWNSIMEGLVAGVPVVACPQFSDQTTNAKLVEVWGTGVKARANEEGVVEREEIKKCLEMVMEGGGKGEEMRRNAEKWKGLAVECMECGSSGNINLKHFVESLEIRTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E251</accession>
    <name>A0AA39E251_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">SKP1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_002835</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SKP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E251"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009867">
        <property type="term" value="P:jasmonic acid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18322">
        <property type="entry name" value="BTB_POZ_SKP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000297">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016897">
        <property type="entry name" value="SKP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001232">
        <property type="entry name" value="SKP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036296">
        <property type="entry name" value="SKP1-like_dim_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016072">
        <property type="entry name" value="Skp1_comp_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016073">
        <property type="entry name" value="Skp1_comp_POZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11165">
        <property type="entry name" value="SKP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01466">
        <property type="entry name" value="Skp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03931">
        <property type="entry name" value="Skp1_POZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00512">
        <property type="entry name" value="Skp1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81382">
        <property type="entry name" value="Skp1 dimerisation domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="241"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="SKP1 component POZ" evidence="6">
        <location>
            <begin position="6"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="SKP1 component dimerisation" evidence="5">
        <location>
            <begin position="106"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="SKP1 component POZ" evidence="6">
        <location>
            <begin position="153"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9704454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="263" mass="29303" checksum="C45BE1C651C8509E" modified="2024-01-24" version="1">MASSNTIALRSSDGETFDLNVVAAMESQVIKGMIEDGCAECAIPLPNVNSKILAKVTEYCKKHVETPKAEEHAVNDELKAWVADLVKVDWNTFVDLVLAANYLNIKSLLDLARQTIVDMTKEMSPDEICKMHILMKEKSLDEICKIYNIKTGLTLRSSDSETFDLDVAVATKSQVIMSAVAMKSQVIEDGCADNAIPLPNVTSNILAKVIEYCKKHAVNDELGAWDADFVKVDLATLFDLIMVRLLNLFFFFVVPIFFFLFFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E4S3</accession>
    <name>A0AA39E4S3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Thaumatin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_001879</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E4S3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.110.10">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037176">
        <property type="entry name" value="Osmotin/thaumatin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001938">
        <property type="entry name" value="Thaumatin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31048">
        <property type="entry name" value="OS03G0233200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00314">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002703">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00205">
        <property type="entry name" value="THN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49870">
        <property type="entry name" value="Osmotin, thaumatin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51367">
        <property type="entry name" value="THAUMATIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="22"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="30"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="44"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="54"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR002703-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9706630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="113" mass="12127" checksum="FB1CDE075BFE2D85" modified="2024-01-24" version="1">MDLMCLLAFNPTSNGCTRGISCTADIVGQCPAELKTAGGCNNPCTVFKTDEYCCSSGSCSATTYSKFFKDRCPDAYSYPKDDQTSTFTCPAGTNYDVIFCPSALPQTCVFIIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E725</accession>
    <name>A0AA39E725_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">F-box protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_000760</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E725"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013187">
        <property type="entry name" value="F-box-assoc_dom_typ3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017451">
        <property type="entry name" value="F-box-assoc_interact_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050796">
        <property type="entry name" value="SCF_F-box_component"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01640">
        <property type="entry name" value="F_box_assoc_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31672">
        <property type="entry name" value="BNACNNG10540D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31672:SF13">
        <property type="entry name" value="F-BOX PROTEIN CPR30-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08268">
        <property type="entry name" value="FBA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="3"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="F-box associated beta-propeller type 3" evidence="2">
        <location>
            <begin position="94"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9708924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="382" mass="43871" checksum="458410CEFEE2C498" modified="2024-01-24" version="1">MAWSSLHTDVMLDFFSRLTVKGLHRCRCVSREWLSVISNPNLRTLHRQRWSQHPLMLVLDNINSTTYVITVSDMGGAVLNHFIVDLPVTTSFAIIGYTYCELICFSLNHDKLYVCNPSTQELAEVPNSPYSSRFSASLPPMGFFTLTKQRHILAIGRVPSLDQYKIVRLFMCNVEKNGAYDFGCEVFTIRDGEHVDSGSWKLIGDCPCSVSQTSHASVEGAIYWPVADHALRSQRNHILRLDLETEMFEFISGPQDYLYGLDHEDNDFISFSLQELKGCVCLVNRYQPFFVLDVWMLKDRASGIWVKEHRIDLPPLDHHCMIQGFIPSNHDDGNLLIFMSSNQTYYYYNIQTQTFSVADNLNAEGLHSFFVYYDNFFSLGTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E9P1</accession>
    <name>A0AA39E9P1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_000337</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E9P1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9708232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="122" mass="13785" checksum="3B7C1D10336D4D73" modified="2024-01-24" version="1">MNRKAGYGLQKKIENRKRGRKEPAEKKKKNLEGEQEKRGCRRWGENGEKKTEEERAGRRGKSDSRKHSRHLPINDEGEFRKWQKEVREAEALKSGSISGGAGGDFGRDAGCFKAAGELEKAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YH50</accession>
    <name>A0AA38YH50_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_026729</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YH50"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000344">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000396">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At2g36730"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000090">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24015">
        <property type="entry name" value="OS07G0578800 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24015:SF1753">
        <property type="entry name" value="PPR CONTAINING PLANT-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="166"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="268"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="369"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9670365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="602" mass="66859" checksum="C5A880A125A0F3E7" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YJN1</accession>
    <name>A0AA38YJN1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_025389</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YJN1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34689">
        <property type="entry name" value="NUCLEIC ACID-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34689:SF1">
        <property type="entry name" value="NUCLEIC ACID-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9671657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="151" mass="18276" checksum="A4E7A7F8B2588EB5" modified="2024-01-24" version="1">MWNKRPEFTAWLAEIKQVNLESLPNWEEKQMFKEFMEDHNTATFTSKKYYNIDAYYRRKMEREMKKGSKKVLATERTVFNDEEQRRLELQRERERYKEVQVEELKRSMQSGMAQAMKEQAQLREEMAYQYKVGNSEAAAAIQRRLDPDVAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YKB0</accession>
    <name>A0AA38YKB0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">PGG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_025600</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YKB0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026961">
        <property type="entry name" value="PGG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24186:SF46">
        <property type="entry name" value="PROTEIN ACCELERATED CELL DEATH 6-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24186">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13962">
        <property type="entry name" value="PGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="589"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="630"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="668"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="701"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="41"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="75"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="119"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="193"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="336"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="PGG" evidence="10">
        <location>
            <begin position="585"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="425"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="490"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="490"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="525"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9672028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="729" mass="81467" checksum="5D307BF8C3AB943E" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YLJ4</accession>
    <name>A0AA38YLJ4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cytochrome P450 CYP72A219-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_026048</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YLJ4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20642">
        <property type="entry name" value="CYP72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000029">
        <property type="entry name" value="Cytochrome P450 734A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050665">
        <property type="entry name" value="Cytochrome_P450_Monooxygen"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24282:SF255">
        <property type="entry name" value="CYTOCHROME P450 72A11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24282">
        <property type="entry name" value="CYTOCHROME P450 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11 12" id="KW-0349">Heme</keyword>
    <keyword evidence="8 11 12" id="KW-0408">Iron</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 12" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="11">
        <location>
            <position position="462"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9672673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="514" mass="59141" checksum="7B695640F3D539C3" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YLV9</accession>
    <name>A0AA38YLV9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">MADS-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_026216</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YLV9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11945:SF725">
        <property type="entry name" value="AGAMOUS-LIKE 58-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11945">
        <property type="entry name" value="MADS BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00404">
        <property type="entry name" value="MADSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="6">
        <location>
            <begin position="7"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9672870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="147" mass="16775" checksum="38B00B473D80016F" modified="2024-01-24" version="1">MEKKQTKGRQKIEMKRIPKEEDPLITFLKHRFGIYNKVSELSTLCGAGVGVLVFSPAGKAFLFGHSSIETITNKVLYENPPPNDNTLNLEREKILRKKVPNRSKGWWEKPITNELRTRASSSSLPFSIVNQTPPTNPFSMTKVEQKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YM66</accession>
    <name>A0AA38YM66_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Retrotransposon Copia-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_026326</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9673002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YM66"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029472">
        <property type="entry name" value="Copia-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34222:SF40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34222">
        <property type="entry name" value="GAG_PRE-INTEGRS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14244">
        <property type="entry name" value="Retrotran_gag_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Retrotransposon Copia-like N-terminal" evidence="2">
        <location>
            <begin position="26"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9673002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="294" mass="33637" checksum="49EF0440358B7360" modified="2024-01-24" version="1">MDAQRGNNYERVSEIHSSMGPVGAFDNSPLHLTVEKLNDKNYREWAQAIKLVIDGKGKLGFLTGETRRSPPIDVAASQKWRSENSFITSCLINSMKLAIGNTYMFLLTTKDTRLWQMKQGDWEVTEYYTEMLGLWQDLDLSCEEEWECMGDSVRFKKKMENERVFEFLAGLNRELDDVRSRVLSRWPLPSIREVFFEVRREENKRRVMLDHSFGLEGFAFLTYGPHGPHATAGRGLHAAASGPHAVGTSGPSPRQSKRTYCEHCKKLGHTKDTCWALHGKPADWKPRQSKAQSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YTG8</accession>
    <name>A0AA38YTG8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PVL29_025085</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 20">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the RING-type zinc finger family. ATL subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9676386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YTG8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16461">
        <property type="entry name" value="RING-H2_EL5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000503">
        <property type="entry name" value="RING-H2 finger protein ATL7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044600">
        <property type="entry name" value="ATL1/ATL16-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46913:SF23">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RHA4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46913">
        <property type="entry name" value="RING-H2 FINGER PROTEIN ATL16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="17">
        <location>
            <begin position="100"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="163"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="164"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="206"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ9676386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="220" mass="24953" checksum="CB0A1F041D36A541" modified="2024-01-24" version="1">MSYSYSDPPNCCSASAELKLYQAFIFSVPIFFTFILLLLFYLFYLRRRRVDWASLRMRSSMQNDADHASGVSELGLKKEVREMLPIIVFKESFSVSDTQCSVCLADYQAEDRLQQIPACGHTFHMDCIDHWLATHTTCPLCRLSLTAAARTSAEPLTILVETPHGTSPEQTQPDTQVSQPRNENERAVHTTMEAEGGSSECDDCETETRDARNENERHEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YUV1</accession>
    <name>A0AA38YUV1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_022228</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YUV1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000084">
        <property type="entry name" value="Wall-associated receptor kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000043">
        <property type="entry name" value="Wall-associated receptor kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045274">
        <property type="entry name" value="WAK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025287">
        <property type="entry name" value="WAK_GUB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27005:SF515">
        <property type="entry name" value="WALL-ASSOCIATED RECEPTOR KINASE-LIKE 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27005">
        <property type="entry name" value="WALL-ASSOCIATED RECEPTOR KINASE-LIKE 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13947">
        <property type="entry name" value="GUB_WAK_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 15" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041347479" description="Protein kinase domain-containing protein" evidence="15">
        <location>
            <begin position="20"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="368"/>
            <end position="636"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9677121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="636" mass="71623" checksum="6A1D8F6A4AEC2F31" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YW84</accession>
    <name>A0AA38YW84_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Rx N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_022683</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YW84"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14798">
        <property type="entry name" value="RX-CC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038005">
        <property type="entry name" value="RX-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF1136">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RPM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="4">
        <location>
            <begin position="5"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="5">
        <location>
            <begin position="166"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="6">
        <location>
            <begin position="281"/>
            <end position="605"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9677851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="656" mass="74326" checksum="81737E9D53757FBA" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YXM9</accession>
    <name>A0AA38YXM9_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_020697</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YXM9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14154">
        <property type="entry name" value="UPF0041 BRAIN PROTEIN 44-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103511">
        <property type="entry name" value="Chlorophyll a-b binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="168"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="196"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="18"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="62"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="240"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9678600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="262" mass="28999" checksum="1921172A12EB4F11" modified="2024-01-24" version="1">MASSIAITASLQRACCPHHVTKKQQPQARPARSMGTKQETHVVALETEDPKGLNIAEQEAKFGFNAGSSTTTSRDAKDDDEAGSGLDTEPSAPKFSDERWKNGTWDLNMFVRDGKMDWDGVIVAEARRRKFLEMYPESATNQEPVLFRSSIIPWWAWMMRSHLPEAELINGRAAMVGFFMAYLVDALTGLDVVGQTGNLICKAGLLVTVMGVIFFRRAQDFDSLRKLADEATLYDKQWESSWQDQDAGDAGDGDSGRGGKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z0E0</accession>
    <name>A0AA38Z0E0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aldehyde dehydrogenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_019360</name>
        <name evidence="3" type="ORF">PVL29_023807</name>
        <name evidence="2" type="ORF">PVL29_026377</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9673066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z0E0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016620">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="1">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9673066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9674474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9680055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="44" mass="5070" checksum="3DC7F30AB06D40A8" modified="2024-01-24" version="1">MKETLRFLDTNMGPTIRSDVTTSMLCHKEEIFGPILLCMGQKWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z194</accession>
    <name>A0AA38Z194_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_019756</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z194"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF78">
        <property type="entry name" value="OS12G0587133 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9680523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="248" mass="28525" checksum="0CE3F4EF7A704FEC" modified="2024-01-24" version="1">MPCYFLSLFKIPASVAAKIERLQRDFLWSGVGEGKRDYLVSWDVVCKPKANGGLGFGKIALRNVALLGKWLWRYPREGFALWHKVILGIYGSHSNGWDANTIVRWSHRCPWKAIAQVFQEFSKFTRFVLGDGERIRFWEDLWWGDQPLGSQYPRLFRVVTDKNSPISSILGSTHPFSWNFNFRRNLSDPEIEEFEGLMRSLDCLHVSFSILDARSWSLSSSGLFTVKSFFLALSHCFGSHPVFSTKCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z1L7</accession>
    <name>A0AA38Z1L7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Alpha/beta-Hydrolases superfamily protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_019816</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z1L7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31934">
        <property type="entry name" value="ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31934:SF6">
        <property type="entry name" value="ALPHA_BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9680614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="505" mass="55307" checksum="19AA0921978DB5F3" modified="2024-01-24" version="1">MEGGNPDNSESNTPLLNSGLRNNELISYQSRNDGLIPQLFTSVPALNEAASYLAETTSFLSRCFTDYSVEPAYSVVNEQELTTFPSGQTREYLDSIHRPSRGSYSTLYESYDTETATPPVPDGITRNSPGDTSQNNGALVVTDHPHQNGISMFQGLIDRARRTVRGSADDIGWIQRAPGMPPVEDGTDRFMEIIDEIRHGVHRLPSSMIYLLVPGLFSNHGPLYFVNTKISFSKMGLACHIAKIHSEASVEKNAREIKEYIEEIYWGSQKRVLLLGHSKGGVDAAAALSMYWSDLKDKVAGLVLAQSPYGGSPIASDILREGQLGDYVNLRKVMEILICKVIKGDIQALEDLTYEKRKEFLRKHHLPPALPVVSFITEAGISPAVVATLSRVAHAEVPIVAPLTAGQPAKVPVVMPLGAAMAACAQLLQIRYGEKSDGLVTCRDAQVPGSIVVRPKRKLDHAWMVYSALDDDPTEANASQVCEALLRLLVEAGDRKRHELAMKAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z1U5</accession>
    <name>A0AA38Z1U5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Subtilisin-like protease SBT1.1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_019976</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the peptidase S8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z1U5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02120">
        <property type="entry name" value="PA_subtilisin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04852">
        <property type="entry name" value="Peptidases_S8_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.2310:FF:000001">
        <property type="entry name" value="Subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000006">
        <property type="entry name" value="Subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000005">
        <property type="entry name" value="subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034197">
        <property type="entry name" value="Peptidases_S8_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010259">
        <property type="entry name" value="S8pro/Inhibitor_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045051">
        <property type="entry name" value="SBT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041469">
        <property type="entry name" value="Subtilisin-like_FN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10795">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17766">
        <property type="entry name" value="fn3_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05922">
        <property type="entry name" value="Inhibitor_I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 7" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041206808" description="Subtilisin-like protease SBT1.1" evidence="9">
        <location>
            <begin position="21"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="domain" description="Inhibitor I9" evidence="12">
        <location>
            <begin position="27"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="10">
        <location>
            <begin position="135"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="11">
        <location>
            <begin position="372"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="Subtilisin-like protease fibronectin type-III" evidence="13">
        <location>
            <begin position="658"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="584"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6 7">
        <location>
            <position position="143"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6 7">
        <location>
            <position position="217"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6 7">
        <location>
            <position position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF05922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF17766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9680823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="762" mass="80237" checksum="8A1F5797E10CD92F" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z260</accession>
    <name>A0AA38Z260_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Actin-related protein 2/3 complex subunit 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_019800</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Functions as a component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Arp2/3 complex plays a critical role in the control of cell morphogenesis via the modulation of cell polarity development.</text>
    </comment>
    <comment type="function">
        <text evidence="9">Functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Arp2/3 complex plays a critical role in the control of cell morphogenesis via the modulation of cell polarity development.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the Arp2/3 complex composed of ARP2, ARP3, ARPC1/p41-ARC, ARPC2/p34-ARC, ARPC3/p21-ARC, ARPC4/p20-ARC and ARPC5/p16-ARC.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the ARPC5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z260"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005885">
        <property type="term" value="C:Arp2/3 protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.190:FF:000002">
        <property type="entry name" value="Actin-related protein 2/3 complex subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.190">
        <property type="entry name" value="Actin-related protein 2/3 complex subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006789">
        <property type="entry name" value="ARPC5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036743">
        <property type="entry name" value="ARPC5_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12644">
        <property type="entry name" value="ARP2/3 COMPLEX 16 KD SUBUNIT P16-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04699">
        <property type="entry name" value="P16-Arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69103">
        <property type="entry name" value="Arp2/3 complex 16 kDa subunit ARPC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00059196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00065908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9680594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="135" mass="15227" checksum="F8177701D3588C86" modified="2024-01-24" version="1">MAGTKEFVEADNAEAIITRIEHKSRKIESLLKQQKPIEALKTALEGSPPNTRDERCKSANWIVVHRAMMAIKDVDSLFSSLDPEYYDILMKYLYRGLSTGDRPTCDQCLRIHEKLTEKAGLGCILRSLADTVNTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z2J6</accession>
    <name>A0AA38Z2J6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_020046</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z2J6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016132">
        <property type="term" value="P:brassinosteroid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010268">
        <property type="term" value="P:brassinosteroid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016125">
        <property type="term" value="P:sterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286">
        <property type="entry name" value="CYTOCHROME P450 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286:SF185">
        <property type="entry name" value="CYTOCHROME P450 87A3-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0349">Heme</keyword>
    <keyword evidence="6 7" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041357780" description="Cytochrome P450" evidence="8">
        <location>
            <begin position="18"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9680923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="397" mass="45414" checksum="529A7C469668C4A3" modified="2024-01-24" version="1">MWMLVICLFTLVVILLSQWLQKWLNPKGNGRLPRASMACQPLERRSSIFSMVLFSLNFRYGSVFKTRLLNQYIFKEEGKSVYCSYTESALKVMGEQSLLAYHGVFHKYLKNLTLSMIVPESLKEVLLHEMDAITRKYLHSCSSYASFDVKDESANLQSFSDGIISFPLNIPGTAFHVCLRGCENVVKVINERKSSKKPYHDFLDFLLERGTIIVDLVFVLLFASYKTTFETITLVMKFLSDHLSIVVELMKEHEEIMKNRGNKNRELHGLGNIILGIFRVTKDIEMKVMVCPSAVHLNPAKYNDPLAFDPWPWEGQELHAGSKNFIAFGGGSRLCAGAHFATVQVSVFLHCLVTKYRNNKTFYCFKWKKKPLGGEIIQKAGLVFSHLLHIQISVKYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z5H1</accession>
    <name>A0AA38Z5H1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Aminotransferase-like plant mobile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_018643</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z5H1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34835">
        <property type="entry name" value="OS07G0283600 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34835:SF34">
        <property type="entry name" value="OS08G0555500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="242"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9682761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="430" mass="49180" checksum="0BB70721512BBBEA" modified="2024-01-24" version="1">MQSTRLYTRCSSSRFLKLCNRLPAEKLDVEIRHNICIWLIDYFNVGFRRIDITSHKRYDLTTTDVGLVFGLPTTGRILHIATTPSDRLFGTLNTCEERLLNLPIREEFRRCFIYYTCAMLLAPTSRIDGYRNLWHTIHEDGFRNDVNWGQFVVDQLVEGIRRFKQRNSIWVHGCILFLQYQQVRIIHMFISMVQLHYVIKFKIPSVHVPMTAPLLSAWSDELIKERLSAEISEFGSFGHGEGFDESSPARTHVEDESGQTSSHQQLGIMRGLIHRLDAQRERGVHSPIAGHSGYAADDFPEADHDSPYARYDMPFHGTEEVLDTPIRHPPIIADDEVVVCDPLPLRSMTVARSHKSMDLKEAAAIVFDGDLDPSEELVSMHDTSLTKGNLASFQGDCWIGIDVNISICPLVCIILNFNLMYHLLISQSEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z663</accession>
    <name>A0AA38Z663_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_018822</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z663"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19501">
        <property type="entry name" value="RecA-like_FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000277">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000061">
        <property type="entry name" value="Probable inactive ATP-dependent zinc metalloprotease FTSHI 4, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23076:SF110">
        <property type="entry name" value="INACTIVE ATP-DEPENDENT ZINC METALLOPROTEASE FTSHI 3, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23076">
        <property type="entry name" value="METALLOPROTEASE M41 FTSH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0934">Plastid</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="15">
        <location>
            <begin position="356"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9682985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="612" mass="68082" checksum="81AC7DECCF289D74" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z6C8</accession>
    <name>A0AA38Z6C8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_018880</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z6C8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010038">
        <property type="term" value="P:response to metal ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.430:FF:000003">
        <property type="entry name" value="FERONIA receptor-like kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.430:FF:000007">
        <property type="entry name" value="FERONIA receptor-like kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000252">
        <property type="entry name" value="Receptor-like protein kinase FERONIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000645">
        <property type="entry name" value="Receptor-like protein kinase FERONIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.430">
        <property type="entry name" value="Galactose-binding lectin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045272">
        <property type="entry name" value="ANXUR1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024788">
        <property type="entry name" value="Malectin-like_Carb-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34590">
        <property type="entry name" value="OS03G0124300 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34590:SF5">
        <property type="entry name" value="OS04G0586500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12819">
        <property type="entry name" value="Malectin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 15" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041209399" description="Protein kinase domain-containing protein" evidence="15">
        <location>
            <begin position="30"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="449"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="529"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="54"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="421"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="829"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="68"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="835"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="857"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="558"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9683075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="897" mass="99766" checksum="40F84AF02D7BF1EF" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z6T2</accession>
    <name>A0AA38Z6T2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_018888</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z6T2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010038">
        <property type="term" value="P:response to metal ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.430:FF:000003">
        <property type="entry name" value="FERONIA receptor-like kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.430:FF:000007">
        <property type="entry name" value="FERONIA receptor-like kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000252">
        <property type="entry name" value="Receptor-like protein kinase FERONIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000645">
        <property type="entry name" value="Receptor-like protein kinase FERONIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.430">
        <property type="entry name" value="Galactose-binding lectin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045272">
        <property type="entry name" value="ANXUR1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024788">
        <property type="entry name" value="Malectin-like_Carb-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34590">
        <property type="entry name" value="OS03G0124300 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34590:SF15">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12819">
        <property type="entry name" value="Malectin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="448"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="530"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="421"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="839"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="427"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="846"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="559"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9683083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="873" mass="96938" checksum="FCC75A9AFC79198D" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z7Q1</accession>
    <name>A0AA38Z7Q1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Prephenate/arogenate dehydrogenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_016447</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9683955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z7Q1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033730">
        <property type="term" value="F:arogenate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008977">
        <property type="term" value="F:prephenate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004665">
        <property type="term" value="F:prephenate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006571">
        <property type="term" value="P:tyrosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046826">
        <property type="entry name" value="PDH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003099">
        <property type="entry name" value="Prephen_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045011">
        <property type="entry name" value="TYRAAT1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059064">
        <property type="entry name" value="TYRAAT2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43207:SF3">
        <property type="entry name" value="AROGENATE DEHYDROGENASE 1, CHLOROPLASTIC-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43207">
        <property type="entry name" value="AROGENATE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02153">
        <property type="entry name" value="PDH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26213">
        <property type="entry name" value="TYRAAT1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51176">
        <property type="entry name" value="PDH_ADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Prephenate/arogenate dehydrogenase" evidence="3">
        <location>
            <begin position="13"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="237"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9683955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="272" mass="30607" checksum="202DCA378466381D" modified="2024-01-24" version="1">MAVSSSSPLSRTLRIGIVGFGPFGQFLATTMMKQGHTLTATSRSDHSQLCARLGISFFRGMNEFIEAENDVIMLCTSILSLTEVLKSLPLHCLKRPTLFADVLSVKEGPREVLLQVLPEESDVLCTHPMFGPESGRDGWNGLAFMYERVRIRDEATCSSFLHIFESEGCRMLEMSCEEHDKLAARSQFLTHTIGRILSEMEIESTPIDTKGFQTLIQLKDSTIRDSFDLYSGLFVHNKFAKQELNNLALAFEKVKQKLEEMNEKSDLSMQPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z885</accession>
    <name>A0AA38Z885_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_016517</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the major facilitator superfamily. Phosphate:H(+) symporter (TC 2.A.1.9) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z885"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17364">
        <property type="entry name" value="MFS_PhT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000175">
        <property type="entry name" value="Inorganic phosphate transporter 1-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0769">Symport</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="113"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="150"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="200"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="282"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="346"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="388"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="460"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="11">
        <location>
            <begin position="24"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="487"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9684062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="509" mass="55582" checksum="8D0FF1FA391EF309" modified="2024-01-24" version="1">MADGSQAIFSALDNAKTQLYHFKAIVIAGMGFFTDAYDLFCITAVTKLIGRLYYYDPIKNEPGKLPNNVSNAITGVALCDKLGRKHVYGITLVTMVGCAVASGLSFGSTAHSVIASLCFFRFWLGFGIGGDYPLSAVIMSEYANQKTRGAFIAAVFAMQGIGILVAGAVSMIVSKAFLVAYKAEVFSENHVLSTQPQGDFVWRIVLMFGAVPAMLTYYWRMKMPETARFTALVKGNHEKAAADMAKVLEQYIVVEESKPKLPVTPSPSYGLLSREFVKRHGLQLLGTSSTWFLLDIAFYSLQLTQKDIYPATGLVPKASTMNAIEEMYKISKAMFLVALFATVPGYWFTLGGFLLMSIFMAVIGFLYDDLRGDKNKCPRESTDEFCEGKPYLFAVFYGLTLFFANFGPNSTTFIVPAELFPARFRSTCHGISAAAGKAGAIIGAFLVQSYTQNVDDIKRAIVALSVVNLIGFFFTFLVPETKGKSLEQLSGEDQNGGDGEGVVKTDEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z9H0</accession>
    <name>A0AA38Z9H0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_016780</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z9H0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000344">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000393">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At1g20230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000090">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF347">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13812">
        <property type="entry name" value="PPR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="53"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="119"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="220"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="321"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="458"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="493"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="594"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="629"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="731"/>
            <end position="765"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9684478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="958" mass="107371" checksum="17990E7712A663E2" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2025-04-02" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZAB7</accession>
    <name>A0AA38ZAB7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Mediator complex subunit 15 KIX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_017281</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.246.20:FF:000003">
        <property type="entry name" value="Mediator of RNA polymerase II transcription subunit 15a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.20">
        <property type="entry name" value="Coactivator CBP, KIX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036529">
        <property type="entry name" value="KIX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036546">
        <property type="entry name" value="MED15_KIX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044661">
        <property type="entry name" value="MED15a/b/c-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33137">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 15A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33137:SF4">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 15A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16987">
        <property type="entry name" value="KIX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47040">
        <property type="entry name" value="Kix domain of CBP (creb binding protein)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mediator complex subunit 15 KIX" evidence="5">
        <location>
            <begin position="4"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="75"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="164"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="300"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="444"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="502"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="567"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="683"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="936"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1040"/>
            <end position="1065"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="251"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="102"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="164"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="201"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="300"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="342"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="505"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="692"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="943"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="1050"/>
            <end position="1059"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF16987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9685187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1422" mass="158105" checksum="B7E66B15E801A25C" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZB94</accession>
    <name>A0AA38ZB94_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Disease resistance RPP13-like protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_017442</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="RX-CC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
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        <property type="entry name" value="Apaf_helical"/>
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        <property type="entry name" value="LRR_R13L1-DRL21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
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    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038005">
        <property type="entry name" value="RX-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766:SF51">
        <property type="entry name" value="DISEASE RESISTANCE RPP13-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766">
        <property type="entry name" value="PLANT BROAD-SPECTRUM MILDEW RESISTANCE PROTEIN RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25019">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
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        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0611">Plant defense</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="8"/>
            <end position="101"/>
        </location>
    </feature>
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        <location>
            <begin position="184"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="8">
        <location>
            <begin position="441"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="domain" description="R13L1/DRL21-like LRR repeat region" evidence="9">
        <location>
            <begin position="703"/>
            <end position="830"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF25019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9685407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1390" mass="157505" checksum="AE86A8F72AC95851" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZCD3</accession>
    <name>A0AA38ZCD3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_015366</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZCD3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032440">
        <property type="term" value="F:2-alkenal reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000121">
        <property type="entry name" value="Prostaglandin reductase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041694">
        <property type="entry name" value="ADH_N_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045010">
        <property type="entry name" value="MDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43205:SF80">
        <property type="entry name" value="2-ALKENAL REDUCTASE (NADP(+)-DEPENDENT)-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43205">
        <property type="entry name" value="PROSTAGLANDIN REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16884">
        <property type="entry name" value="ADH_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="2">
        <location>
            <begin position="77"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9686433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="361" mass="39448" checksum="F614A7DDE7092BB8" modified="2024-01-24" version="1">MSGLEGVGGCGGENSTVVESREWVMSAYASEGVPTSDHLKLRTTKLSLDADSIPDGHVAVELLWISVDPYLRTRMTGHDDGLYFPQFALNEVITAFGVGRVIQSKNGKFSTGDIVVNPFFPFAEYCIAPPTFLRVIDPTAGIELPAYLSSLGVPGFAAWVGIEVLGNPKPESNVFISAAAGGVGMFAGQLAKLKGCRVVGSTGTDEKVKLLKEEFGYDEAFNYKKETDFDAALSKYLPNGIDLYLDNVGGKMLEAVLNHVNLHARIPICGMISQYNQIWTEREGVRNLLNMVGKEVRMEGYLVGSYLDRFDDFTKEMETYTKEGKLRSKLKIYSGIESFIESLGSMFSSSNIGKVVIQVKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZD97</accession>
    <name>A0AA38ZD97_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">glucan endo-1,3-beta-D-glucosidase</fullName>
            <ecNumber evidence="4">3.2.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PVL29_015663</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;3)-beta-D-glucosidic linkages in (1-&gt;3)-beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.39"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the glycosyl hydrolase 17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.39" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZD97"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000008">
        <property type="entry name" value="Glucan endo-1,3-beta-glucosidase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1040:FF:000002">
        <property type="entry name" value="Glucan endo-1,3-beta-glucosidase 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000490">
        <property type="entry name" value="Glyco_hydro_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044965">
        <property type="entry name" value="Glyco_hydro_17_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012946">
        <property type="entry name" value="X8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32227">
        <property type="entry name" value="GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00332">
        <property type="entry name" value="Glyco_hydro_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07983">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00768">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0611">Plant defense</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 16" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041246829" description="glucan endo-1,3-beta-D-glucosidase" evidence="16">
        <location>
            <begin position="21"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="domain" description="X8" evidence="17">
        <location>
            <begin position="367"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ9686895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="484" mass="52993" checksum="5FC8222F9B82F536" modified="2024-01-24" version="1">MPPNLCLILLLCISTSTTRATAIGLNWGTAASHPLPPPRVVELLKNNNIARVKLFDADPLVLQALSGSKIAVTVGIPNSMLRSLNSSKKAAESWVHDNVTRYVSSSGRASGVRIEYVAVGDEPFLQSYGDQFQPFVIGAATNIQTALIRANLASEVKVMVPCSSDTIQSESNLPSKGHFRSDLNKTMSHLLTFLNKHHSPFFVNISPFLSLHQNKNISLDFSIFKETAHPHSDSHRTYKNSFDLIYDTVVTALSTVGYQEMDIVVGQIGWPTDGAANATSSIAETFMKGLIRHLQSKSGTPLRPRVPPTETYIFSLLDEDQRSIAAGNFERHWGLFTFDGQAKYHVDLGQGSRNLVNAQNVNYLPSRWCVVNNNRDLSNATASASEACSVADCTALSLGSSCFNISWPANISYAFNSYYQQHNQQAASCDFGGLGLITTVDPSMEKCRFSIQLHTSHSSSLHPLHLLHQMLLAAATILVCFSQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZDC1</accession>
    <name>A0AA38ZDC1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ferroxidase</fullName>
            <ecNumber evidence="3">1.16.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_015588</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>4 Fe(2+) + O2 + 4 H(+) = 4 Fe(3+) + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:11148"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="1.16.3.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the frataxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.16.3.1" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZDC1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008199">
        <property type="term" value="F:ferric iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004322">
        <property type="term" value="F:ferroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034986">
        <property type="term" value="F:iron chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00503">
        <property type="entry name" value="Frataxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.920.10:FF:000003">
        <property type="entry name" value="Frataxin, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.920.10">
        <property type="entry name" value="Frataxin/CyaY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017789">
        <property type="entry name" value="Frataxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002908">
        <property type="entry name" value="Frataxin/CyaY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036524">
        <property type="entry name" value="Frataxin/CyaY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020895">
        <property type="entry name" value="Frataxin_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03421">
        <property type="entry name" value="FeS_CyaY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03422">
        <property type="entry name" value="mito_frataxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16821">
        <property type="entry name" value="FRATAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16821:SF2">
        <property type="entry name" value="FRATAXIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01491">
        <property type="entry name" value="Frataxin_Cyay"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00904">
        <property type="entry name" value="FRATAXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01219">
        <property type="entry name" value="Frataxin_Cyay"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55387">
        <property type="entry name" value="Frataxin/Nqo15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01344">
        <property type="entry name" value="FRATAXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50810">
        <property type="entry name" value="FRATAXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0409">Iron storage</keyword>
    <keyword evidence="6" id="KW-0410">Iron transport</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9686807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="194" mass="21869" checksum="CAAACB4E567B405D" modified="2024-01-24" version="1">MASASSSKLLFLRRLSRALKLSSSSSLSVRPYVFLLEASRPSDLARINFRSFLSVASRSFCSRTLDLEDDSQGPAAIDYRSLVQEDEFHKLADSTIQDLQEKFEEYGDCVQIDGFDIDYGNQVLTLKLGTLGTYVLNKQTPNRQIWLSSPVSGPSRFDWDQSAQAWVYRRTKANLSKLLETELEKLCGIPINLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZDQ3</accession>
    <name>A0AA38ZDQ3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cell wall protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_015479</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZDQ3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034565">
        <property type="entry name" value="Put_cell_wall"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36733">
        <property type="entry name" value="CELL WALL PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36733:SF1">
        <property type="entry name" value="CELL WALL PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041252630" description="Cell wall protein" evidence="2">
        <location>
            <begin position="26"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="71"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9686623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="122" mass="12401" checksum="51DF4A4F2CC98EC8" modified="2024-01-24" version="1">MAYSLQASLLALLVIFNVLLGQAIAAREVPNDSKKVDKKQPEWFIEPDGSLLIPGIGRVMVPGYGSYTPYDGSIPSGSTGSDGSTGGIGSYIPGGDDTFVPNPGVEIPNPAGGGGVPTAARP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZGJ4</accession>
    <name>A0AA38ZGJ4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">phenylalanine ammonia-lyase</fullName>
            <ecNumber evidence="6">4.3.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_013978</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This is a key enzyme of plant metabolism catalyzing the first reaction in the biosynthesis from L-phenylalanine of a wide variety of natural products based on the phenylpropane skeleton.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-phenylalanine = (E)-cinnamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:21384"/>
            <dbReference type="ChEBI" id="CHEBI:15669"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="EC" id="4.3.1.24"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Phenylpropanoid metabolism; trans-cinnamate biosynthesis; trans-cinnamate from L-phenylalanine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PAL/histidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.1.24" evidence="6"/>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZGJ4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045548">
        <property type="term" value="F:phenylalanine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006559">
        <property type="term" value="P:L-phenylalanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009698">
        <property type="term" value="P:phenylpropanoid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.200.10">
        <property type="entry name" value="Fumarase/aspartase (Central domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.274.20">
        <property type="entry name" value="Phenylalanine ammonia-lyase 1, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001106">
        <property type="entry name" value="Aromatic_Lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023144">
        <property type="entry name" value="Phe_NH3-lyase_shielding_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10362">
        <property type="entry name" value="HISTIDINE AMMONIA-LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="L-aspartase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-0585">Phenylalanine catabolism</keyword>
    <keyword evidence="8" id="KW-0587">Phenylpropanoid metabolism</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9688018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="224" mass="25575" checksum="829562B8E79763E6" modified="2024-01-24" version="1">MREIVKHLITQVARKTLYTDEDGTLLESRFCEKELLQVVESLPVFSYLDDPTNPSYSFLPQLRDVLVERALKDPKSTDSAGYSSFKRIPIFQEELEEKLVEKISKARERLDNGGFPITNRIKKCRTYPIYGFVRKEVGTKLLSGENKVSPGEDIEKVYEAINDGKLVDVLMKCLAFWRGTAGPFTPRPSGLSSPAQFNPEYWGWFEELRSPSSASNGRVNWDQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZH99</accession>
    <name>A0AA38ZH99_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DYW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_014604</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. PCMP-H subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9689040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZH99"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010467">
        <property type="term" value="P:gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016071">
        <property type="term" value="P:mRNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000364">
        <property type="entry name" value="Pentatricopeptide repeat (PPR-like) superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000344">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000463">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001104">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032867">
        <property type="entry name" value="DYW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046849">
        <property type="entry name" value="E2_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF452">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14432">
        <property type="entry name" value="DYW_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20430">
        <property type="entry name" value="Eplus_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="171"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="272"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="373"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="443"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="474"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="545"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="domain" description="DYW" evidence="4">
        <location>
            <begin position="689"/>
            <end position="781"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9689040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="781" mass="85584" checksum="3E0B205191C868F0" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZHI2</accession>
    <name>A0AA38ZHI2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Calcineurin-like phosphoesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_014537</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZHI2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07397">
        <property type="entry name" value="MPP_NostocDevT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027629">
        <property type="entry name" value="DevT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04168">
        <property type="entry name" value="TIGR04168 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35769">
        <property type="entry name" value="CALCINEURIN-LIKE METALLO-PHOSPHOESTERASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35769:SF2">
        <property type="entry name" value="CALCINEURIN-LIKE METALLO-PHOSPHOESTERASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="1">
        <location>
            <begin position="33"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9688925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="347" mass="37953" checksum="67404203D1BE13AE" modified="2024-01-24" version="1">MLSAWFHAPTLFILSGSSPSSSSSRLPMATSVRIAVVGDVHDDWNLQEDSKALQFLQPDLVLFTGDFGNENVELVRSVANLEMAKAVILGNHDAWTTQQFSGKKKDGVQLQLECLGKEHVGYQRMDFPVLKLSVVGGRPFSCGGKQLFRKRLLSARYGIHDMEASTKRIYETVLGTPEDHVVIFLAHNGPTGLGSNMNDICGVDWVYGGGDHGDPDLAQAISHLKETTKFSIPLVVFGHMHKELAHGNGHRKMIVVGEGNTTYLNGAIVPRVKTLINEQGTSNTSVTNSEAPPFTPESKGTIRAFTVVEILDGRLDKIAETWVSVIGDETRVEQEHILFKSGMELSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZKF7</accession>
    <name>A0AA38ZKF7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Receptor-like serine/threonine-protein kinase</fullName>
            <ecNumber evidence="16">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">PVL29_012741</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="25">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="25">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="16"/>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZKF7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048544">
        <property type="term" value="P:recognition of pollen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00028">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01098">
        <property type="entry name" value="PAN_AP_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000467">
        <property type="entry name" value="Liguleless narrow1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001037">
        <property type="entry name" value="Putative G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.10">
        <property type="entry name" value="Bulb-type lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021820">
        <property type="entry name" value="S-locus_recpt_kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000858">
        <property type="entry name" value="S_locus_glycoprot_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024171">
        <property type="entry name" value="SRK-like_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002">
        <property type="entry name" value="RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD1-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002:SF1055">
        <property type="entry name" value="RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11883">
        <property type="entry name" value="DUF3403"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08276">
        <property type="entry name" value="PAN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00954">
        <property type="entry name" value="S_locus_glycop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000641">
        <property type="entry name" value="SRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00473">
        <property type="entry name" value="PAN_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="17" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 16" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 20" id="KW-0732">Signal</keyword>
    <keyword evidence="4 16" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041235861" description="Receptor-like serine/threonine-protein kinase" evidence="20">
        <location>
            <begin position="27"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="424"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="23">
        <location>
            <begin position="27"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="22">
        <location>
            <begin position="274"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="24">
        <location>
            <begin position="329"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="504"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="799"/>
            <end position="822"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR000641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="KAJ9690237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="822" mass="92037" checksum="6FC5EA38EE14C597" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZKF8</accession>
    <name>A0AA38ZKF8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Plant bHLH transcription factor ACT-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_013076</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZKF8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054502">
        <property type="entry name" value="bHLH-TF_ACT-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051358">
        <property type="entry name" value="TF_AMS/ICE1/BHLH6-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31945:SF27">
        <property type="entry name" value="TRANSCRIPTION FACTOR BHLH35-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31945">
        <property type="entry name" value="TRANSCRIPTION FACTOR SCREAM2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22754">
        <property type="entry name" value="bHLH-TF_ACT-like_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Plant bHLH transcription factor ACT-like" evidence="3">
        <location>
            <begin position="63"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9690736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="150" mass="16997" checksum="846D5EC9E6939CD9" modified="2024-01-24" version="1">MESRFRKRMALRRKLHILRTLTSSKSVKKSSIVMDAFLYIYKLKLKLEAIKREYLKLINYTQEVKVEKINGKGFLVRVSCKKGQDLLVSILEAFEDMGLNVLQARVSCNHGFGMEAVVEAEDQALDGRAVTEAVLKAIEKPGGDEKHVKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZLJ4</accession>
    <name>A0AA38ZLJ4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_013487</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZLJ4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21742">
        <property type="entry name" value="MobB_NDR_LATS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05599">
        <property type="entry name" value="STKc_NDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000042">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000106">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000102">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059233">
        <property type="entry name" value="MobB_NdrA/B/Cbk1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050839">
        <property type="entry name" value="Rho-assoc_Ser/Thr_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22988:SF76">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_135, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22988">
        <property type="entry name" value="MYOTONIC DYSTROPHY S/T KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="116"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="14">
        <location>
            <begin position="422"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="500"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="538"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9691326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="564" mass="65585" checksum="B0804148178A8EE3" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZMW7</accession>
    <name>A0AA38ZMW7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA-directed RNA polymerase I subunit rpa49</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_011226</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic RPA49/POLR1E RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZMW7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009668">
        <property type="entry name" value="RNA_pol-assoc_fac_A49-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14440">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06870">
        <property type="entry name" value="RNA_pol_I_A49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="8"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="18"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9692028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="411" mass="46364" checksum="4D24872F766885EA" modified="2024-01-24" version="1">MEEEEPETSSQTLTLTPTKKRKKKKHVNVKMEVVHEHPEKIPPLVGYFPSGFDPLKPQDHESDPPPTVKVFRNKARANRLQLVVSPNDSKVNFVGTSYSGEAASAQVCTYALGVLDKETQTLKIVPIASNKIFRLEPRVGGSDVPVDEPSSLLKGEQTAEEKAGRMRDLTNLYGTKKSITQAKKLQALKQKDDPRSQKDLDRKLKEVVINKEALESTSAHSATTPQEAYPLDKIIFKGEWDYLLDILDLLQAGAEVESSAYSSFVCNRIQKLKELEDEGEKTTLACIFSYINHLVKYKDRNSMDASSAKHHKIPGILNQKFSSMFALDSDSRRLTEEKKDLLISYVLVLTLYADEFKTDPSDIARDLRMSAVKLRAHFEHLGCKLVSQNKVTMATLPVPLSFPRLRQKRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZPN9</accession>
    <name>A0AA38ZPN9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cell wall hydroxyproline-rich glycoprotein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_011908</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZPN9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000224">
        <property type="entry name" value="Leucine-rich repeat extensin-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000041">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051582">
        <property type="entry name" value="LRR_extensin-like_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32093:SF115">
        <property type="entry name" value="LEUCINE-RICH REPEAT EXTENSIN-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32093">
        <property type="entry name" value="LEUCINE-RICH REPEAT EXTENSIN-LIKE PROTEIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0134">Cell wall</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041325985" description="Cell wall hydroxyproline-rich glycoprotein" evidence="13">
        <location>
            <begin position="29"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="357"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="365"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="378"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="413"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="462"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="496"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="514"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="566"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="590"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="630"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="665"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="695"/>
            <end position="704"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9692991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="704" mass="75260" checksum="8E609618E28F3F2F" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZPW2</accession>
    <name>A0AA38ZPW2_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_012008</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9693096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZPW2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="136"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9693096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="212" mass="24734" checksum="E4177178AB991126" modified="2024-01-24" version="1">MVSKRVPERRSYSSDYRRIGDRRRIESVRVPENKRKKGSYPGDYRRKDIIRASIGEYLTDRESNSCEFHRIQERNDGIRASTGEEIIFGRVPESNRQTGNRIRACTGESKIEIMILSGRVSENVRETGNRIRASTGESKTEMMVSGRVPKKRSYSGEYRRETDRRTINLCEYRRIQDRKDGIRASTREEMVFGRVPGNRRYTKNRIVATSGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZQ51</accession>
    <name>A0AA38ZQ51_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Disease resistance protein RGA3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_011822</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZQ51"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14798">
        <property type="entry name" value="RX-CC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038005">
        <property type="entry name" value="RX-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766:SF45">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766">
        <property type="entry name" value="PLANT BROAD-SPECTRUM MILDEW RESISTANCE PROTEIN RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="6">
        <location>
            <begin position="6"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="5">
        <location>
            <begin position="181"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="7">
        <location>
            <begin position="433"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="8">
        <location>
            <begin position="572"/>
            <end position="870"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9692900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="905" mass="103079" checksum="0F29EF6D32BF1E9C" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZR02</accession>
    <name>A0AA38ZR02_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_009572</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9693681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZR02"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9693681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="77" mass="8561" checksum="D60C691FAD1524D4" modified="2024-01-24" version="1">MSFICSSFPNLNSRRRCLNHMSSHVAKAIARYSASALDLTTTSCFLLFQDIRLPPIKTQYPEVERLSVGEPAQSASV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZU25</accession>
    <name>A0AA38ZU25_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_010682</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ankyrin SOCS box (ASB) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZU25"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045732">
        <property type="term" value="P:positive regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000316">
        <property type="entry name" value="BRCA1-associated RING domain protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051573">
        <property type="entry name" value="Ankyrin-SOCS_box_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24136:SF15">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24136">
        <property type="entry name" value="SOWAH (DROSOPHILA) HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="68"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="101"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="141"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9695316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="201" mass="21497" checksum="921D0D5EFDE0F49D" modified="2024-01-24" version="1">MAVPGRRNGLMEEDDDNDEALFGELIEDEVETPAHLRGLAAAAQLGDVDALRLALDTLNGSIDEPVEDGDTALHLTCLYGFLPCVQLLLERGASLETKDEDGAIPLHDACAGGFTEIVELLINSTNNTECVQRMLESVDVEGDTPLHHAARGEHVGVIRLLLAHGASPTKGNIYGKIPSELADMDAEAKRILEAAAVEHRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZV11</accession>
    <name>A0AA38ZV11_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Disease resistance protein RGA3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_011001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZV11"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14798">
        <property type="entry name" value="RX-CC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038005">
        <property type="entry name" value="RX-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766:SF61">
        <property type="entry name" value="NB-ARC DOMAIN DISEASE RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766">
        <property type="entry name" value="PLANT BROAD-SPECTRUM MILDEW RESISTANCE PROTEIN RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="6">
        <location>
            <begin position="12"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="5">
        <location>
            <begin position="172"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="7">
        <location>
            <begin position="424"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="8">
        <location>
            <begin position="565"/>
            <end position="768"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9695808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="900" mass="102928" checksum="BA9C12166F8E63D2" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZVG8</accession>
    <name>A0AA38ZVG8_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_010524</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZVG8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9695069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="150" mass="17646" checksum="E289E8EA8F52F802" modified="2024-01-24" version="1">MTLHNLELALRVDEPSKPTDVSSTDERSFYERWEHSNRSCLMVMKYTMDKSIKECMSKTKRAKDFLEYVKVNYTKIDKAEITTYLKLLTTTMYDGVSGVRDHIIKLKHYFNKANEMKVGLNEKFLKWLILESLPTSFDAVKLTYNALKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZX43</accession>
    <name>A0AA38ZX43_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DUF659 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_008931</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZX43"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007021">
        <property type="entry name" value="DUF659"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008906">
        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32166:SF74">
        <property type="entry name" value="OS05G0256350 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32166">
        <property type="entry name" value="OSJNBA0013A04.12 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04937">
        <property type="entry name" value="DUF659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF659" evidence="2">
        <location>
            <begin position="150"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="HAT C-terminal dimerisation" evidence="3">
        <location>
            <begin position="524"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="662"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="666"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="691"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="715"/>
            <end position="728"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9696936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="728" mass="83369" checksum="3ED0BD8A85541815" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZYR3</accession>
    <name>A0AA38ZYR3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Patatin</fullName>
            <ecNumber evidence="5">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_008770</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Lipolytic acyl hydrolase (LAH).</text>
    </comment>
    <comment type="domain">
        <text evidence="5">The nitrogen atoms of the two glycine residues in the GGXR motif define the oxyanion hole, and stabilize the oxyanion that forms during the nucleophilic attack by the catalytic serine during substrate cleavage.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the patatin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="5"/>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZYR3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004620">
        <property type="term" value="F:phospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1090.10:FF:000052">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32176:SF109">
        <property type="entry name" value="PATATIN-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32176">
        <property type="entry name" value="XYLOSE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 4 5" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3 4 5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="407"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="PNPLA" evidence="7">
        <location>
            <begin position="19"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXGXXG" evidence="4">
        <location>
            <begin position="23"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="4">
        <location>
            <begin position="61"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DGA/G" evidence="4">
        <location>
            <begin position="231"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9696719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="429" mass="47367" checksum="BF71ED34EF310988" modified="2024-01-24" version="1">MASTQGSSPRTNRKVITILSIDGGGVRGIIPAVILSALEAELQRLDGPDARIADYFDLIAGTSTGSIVTAFLTTPYPLPNASNGSIANRPREAKDIQNFYIEHGPEIFAKKEDPVQTRKSDGLLARLTELIVHGVEKVLEFLLDYRYGPSSLSEKVGEQLGEIRLADTLTNVLVPAYDIQHLKLVTFSSHQARKADSSVKLRDVVMSSAAAPVYFPSHNFKADGRMYNLVDGGVAANNPTLLAIQEAAHIFGNRDYNYLILSLGTCSEEEEHQNFIQLTGPLPWMIDLKRGTPPLANVLFKTSADMVDAYTLFVLGVGGRISSQNFLRIQDNTLKPEQLTMDDASKENFESLIDIAQRLLEKPVSFPSSVIGPQPMTTNRDALMSFARILSKEKKRRGGSSSHVSGFLIRSIVSFLFLIFLYKLYMMFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZZB1</accession>
    <name>A0AA38ZZB1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycosyltransferase family protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_007274</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZZB1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00761">
        <property type="entry name" value="Glyco_tranf_GTA_type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33604:SF1">
        <property type="entry name" value="GLYCOSYLTRANSFERASE FAMILY PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33604">
        <property type="entry name" value="OSJNBA0004B13.7 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9698085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="931" mass="106149" checksum="9D40EC8CCC1112B4" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A033</accession>
    <name>A0AA39A033_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_007183</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the WIP C2H2-type zinc-finger protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9697947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A033"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000523">
        <property type="entry name" value="Zinc finger protein WIP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055187">
        <property type="entry name" value="C2CH-3rd_BIRD-IDD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043584">
        <property type="entry name" value="WIP1/2/3/4/5/6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059161">
        <property type="entry name" value="Znf-C2H2_STOP1/2_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45878">
        <property type="entry name" value="ZINC FINGER PROTEIN WIP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45878:SF1">
        <property type="entry name" value="ZINC FINGER PROTEIN WIP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22995">
        <property type="entry name" value="C2CH-3rd_BIRD-IDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23115">
        <property type="entry name" value="zf-C2H2_STOP2_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="172"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="256"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9697947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="334" mass="37689" checksum="22FC795551E969BB" modified="2024-01-24" version="1">MTDPYSNYSNGWFNLNPLYHISSSPSSSNQSLCSPRYDFNKLYYNNFNTNNYHYSPPSPPLREALPLLSLGPTRYDEQESSCNTAMEVDMNKGKEEATLSSSADDETVTVALHLGLPSPSSTDLISRLSSSETEDKEEVTVASGYSSTGSTLIKGQYWIPTPSQILIGPTQFACPLCFKTFNRYNNMQMHMWGHGSQYRKGPESLRGTQPTAMLKLPCFCCAPGCRNNIDHPRSRPLKDFRTLQTHYKRKHGIKPFMCRKCGKAFAVRGDWRTHEKNCGKLWYCACGSDFKHKRSLKDHIKAFGHGHAAYGIDCFEEEDEPASEIEQDNESSHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A0M1</accession>
    <name>A0AA39A0M1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8">Cytokinin riboside 5'-monophosphate phosphoribohydrolase</fullName>
            <ecNumber evidence="2 8">3.2.2.n1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_007038</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 8">Cytokinin-activating enzyme working in the direct activation pathway. Phosphoribohydrolase that converts inactive cytokinin nucleotides to the biologically active free-base forms.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>9-ribosyl-trans-zeatin 5'-phosphate + H2O = trans-zeatin + D-ribose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:48564"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16522"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:87947"/>
            <dbReference type="EC" id="3.2.2.n1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 8">
            <text>N(6)-(dimethylallyl)adenosine 5'-phosphate + H2O = N(6)-dimethylallyladenine + D-ribose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:48560"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17660"/>
            <dbReference type="ChEBI" id="CHEBI:57526"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="EC" id="3.2.2.n1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the LOG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.n1" evidence="2 8"/>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9697724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A0M1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016799">
        <property type="term" value="F:hydrolase activity, hydrolyzing N-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009691">
        <property type="term" value="P:cytokinin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.450:FF:000013">
        <property type="entry name" value="Cytokinin riboside 5'-monophosphate phosphoribohydrolase LOG8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005269">
        <property type="entry name" value="LOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031100">
        <property type="entry name" value="LOG_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00730">
        <property type="entry name" value="Rossman fold protein, TIGR00730 family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31223:SF56">
        <property type="entry name" value="CYTOKININ RIBOSIDE 5'-MONOPHOSPHATE PHOSPHORIBOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31223">
        <property type="entry name" value="LOG FAMILY PROTEIN YJL055W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03641">
        <property type="entry name" value="Lysine_decarbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102405">
        <property type="entry name" value="MCP/YpsA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0203">Cytokinin biosynthesis</keyword>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9697724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="205" mass="22230" checksum="90A4D607AFA3A08F" modified="2024-01-24" version="1">MGKFKRVCVYCGSNSGNRKIFSDAALDLGREMVERRMDLIYGGGSVGLMGLVSQKVYDGGCHVLGVIPRALVPIEISGHAVGQVVIVSDMHERKAEMASRADAFIALPGGYGTMEELLEVIAWAQLGIHDKPVGLLNVDGYYDCLLGLFDKGVEEGFIKPSARNIVLSAKTARELIQKMEDYIPFHDQVAPTQSWNAGEPNATNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A0N1</accession>
    <name>A0AA39A0N1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_007049</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9697739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A0N1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9697739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="61" mass="7133" checksum="2702D2DDF9F3FD22" modified="2024-01-24" version="1">MREFRMIMRIGTETRFTHSQSYENSHVFGMNSQTGDDPFKLLFPLLHAFHPSGNSFKTQLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A0Q0</accession>
    <name>A0AA39A0Q0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cation/H(+) antiporter 14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_007742</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the monovalent cation:proton antiporter 2 (CPA2) transporter (TC 2.A.37) family. CHX (TC 2.A.37.4) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A0Q0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006885">
        <property type="term" value="P:regulation of pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057291">
        <property type="entry name" value="CHX17_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057290">
        <property type="entry name" value="CHX17_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050794">
        <property type="entry name" value="CPA2_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32468">
        <property type="entry name" value="CATION/H + ANTIPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32468:SF23">
        <property type="entry name" value="CATION_H(+) ANTIPORTER 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23256">
        <property type="entry name" value="CHX17_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23259">
        <property type="entry name" value="CHX17_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0630">Potassium</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="66"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="103"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="132"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="166"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="207"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="238"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="271"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="311"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="380"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="415"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="11">
        <location>
            <begin position="48"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H(+) antiporter central" evidence="12">
        <location>
            <begin position="494"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H(+) antiporter C-terminal" evidence="13">
        <location>
            <begin position="631"/>
            <end position="768"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF23256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF23259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9698811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="784" mass="86644" checksum="79F223B58B69A894" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A2E1</accession>
    <name>A0AA39A2E1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Bet v I/Major latex protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_005523</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the BetVI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9699693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A2E1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010427">
        <property type="term" value="F:abscisic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009738">
        <property type="term" value="P:abscisic acid-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07816">
        <property type="entry name" value="Bet_v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.530.20:FF:000007">
        <property type="entry name" value="Major pollen allergen Bet v 1-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000916">
        <property type="entry name" value="Bet_v_I/MLP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024949">
        <property type="entry name" value="Bet_v_I_allergen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050279">
        <property type="entry name" value="Plant_def-hormone_signal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31213">
        <property type="entry name" value="OS08G0374000 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31213:SF55">
        <property type="entry name" value="STRESS-INDUCED PROTEIN SAM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00407">
        <property type="entry name" value="Bet_v_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00634">
        <property type="entry name" value="BETALLERGEN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01037">
        <property type="entry name" value="Bet_v_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00451">
        <property type="entry name" value="PATHOGENESIS_BETVI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0568">Pathogenesis-related protein</keyword>
    <keyword evidence="2 4" id="KW-0611">Plant defense</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bet v I/Major latex protein" evidence="5">
        <location>
            <begin position="1"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9699693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="159" mass="17224" checksum="D75010E889258164" modified="2024-01-24" version="1">MGVITYENEITSSIPPTKMFKAFVLDGDKLIPKILPQAIKCVEIIEGDGGAGTIKKVTFGEGSQFNYVKHRVDGIDKENLAYGYSVIEGDALMGTLESISYEVKFAASPDGGSICKTTSKYHTKGEIDIKEDQIKAGKEKASGMFKAIEAYLLANPNAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A2Z3</accession>
    <name>A0AA39A2Z3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RNA polymerase II subunit A C-terminal domain phosphatase SSU72</fullName>
            <shortName evidence="9">CTD phosphatase SSU72</shortName>
            <ecNumber evidence="9">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_007862</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Protein phosphatase that catalyzes the dephosphorylation of the C-terminal domain of RNA polymerase II. Plays a role in RNA processing and termination.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 12">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the SSU72 phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="9"/>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A2Z3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.550">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006811">
        <property type="entry name" value="RNA_pol_II_suA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20383">
        <property type="entry name" value="RNA POLYMERASE II SUBUNIT A C-TERMINAL DOMAIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04722">
        <property type="entry name" value="Ssu72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 9" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU369031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9698998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="94" mass="10835" checksum="51EEFC40CDB59E81" modified="2024-01-24" version="1">MEAHSLLKKQGFDVSSYGTGAHVKLPGPSLREPNVYEFGTPYKHMFDDLRRKDPDLYPLSSISRFSLFVSREKNISEGKRRNIFFNQMGRVVVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A3J7</accession>
    <name>A0AA39A3J7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Vacuole membrane protein KMS1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_005894</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A3J7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="110"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="265"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="334"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="380"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="9"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9700320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="440" mass="48732" checksum="82851C79CB7E5AF0" modified="2024-01-24" version="1">MGSRKEVASPSSSSSSSGSQDMAISGLCEKQQQEMENLTLTTEPFKVLKFFILAVVQHVKQSVAYLLAKGGWVMLLSVVAVALVFVLMAIDGPHEKHVQELHQYFLFGLWWIALGIASSIGLGSGLHTFVLYLGPHIALFTIKAMQCGRVDIKSAPYDTIRLKSGPSWLDKDCSEFGPPVFSSLHALRVPLSSILPQVQVEAVLWGIGTALGELPPYFISKAASLSGSKSDAIEELDASLPEDDGVVSTHLKQIKRWFLSHSQHLNFFTILVLASVPNPLFDLAGIMCGQFGIPFWKFFLATFIGKAIIKTHIQTVFIISVCNNQLLDWIENELIWVLSLIPGFASVLPNLISKLHAMRDKYLAPSPPVPSNMKVKKWDLSFASIWNTVVWLMLINFFFKIVTATAQNFLKKQQEKELLALKDKLSESTLSNDKSDQVVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A3M7</accession>
    <name>A0AA39A3M7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transcription repressor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Ovate family protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_005927</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Transcriptional repressor that regulates multiple aspects of plant growth and development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A3M7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038933">
        <property type="entry name" value="Ovate"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006458">
        <property type="entry name" value="Ovate_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01568">
        <property type="entry name" value="A_thal_3678"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33057:SF21">
        <property type="entry name" value="TRANSCRIPTION REPRESSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33057">
        <property type="entry name" value="TRANSCRIPTION REPRESSOR OFP7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04844">
        <property type="entry name" value="Ovate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51754">
        <property type="entry name" value="OVATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0678">Repressor</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="OVATE" evidence="8">
        <location>
            <begin position="134"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="24"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="203"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="205"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9700368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="223" mass="24660" checksum="D47A86F98C054892" modified="2024-01-24" version="1">MSSIIWKNFNLCLSKLKCFPTTLPSSPPSKHDHNRHPSSSSSSSISSTSSILIKNFNSLYDLTSDSTSKSLTRTTDDFLSTSEDSVDSESPPDFATVFAAQRFFFSSPGRSNSIFEASESPPESDAIVNGGVAVHTYSPNPYEDFRRSMQEMAEARELRDVAADWDYLHELLLCYLTLNPKHNHKFIIGAFADLIVCLMSSTASDGRRGSADPQRHGASQWFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A3Q9</accession>
    <name>A0AA39A3Q9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CRC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_005961</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the lin-54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A3Q9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005172">
        <property type="entry name" value="CRC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033467">
        <property type="entry name" value="Tesmin/TSO1-like_CXC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044522">
        <property type="entry name" value="TSO1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46159">
        <property type="entry name" value="PROTEIN TESMIN/TSO1-LIKE CXC 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46159:SF12">
        <property type="entry name" value="PROTEIN TESMIN_TSO1-LIKE CXC 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03638">
        <property type="entry name" value="TCR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01114">
        <property type="entry name" value="CXC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51634">
        <property type="entry name" value="CRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRC" evidence="5">
        <location>
            <begin position="500"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="262"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="742"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="745"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="785"/>
            <end position="796"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9700410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="796" mass="86697" checksum="B268F1D7C012E3FC" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A484</accession>
    <name>A0AA39A484_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_006135</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A484"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="13"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="110"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="148"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="187"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="216"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9700671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="281" mass="39390" checksum="3FB5240A2FAE2949" modified="2024-01-24" version="1">MWWLLSLRWFANWGWWLVFWEWLLPLGRFFEWVWFVFRRWLLHWRLVFRRWLLHRRLVFRRWLLHRRLVFRRWLLHRRLVFRRWLLHRRLVFRRWLLHRRFRRWLLHRRLVFRRWLLHRRLMLWWWLLHWRLVFRRWLLHRRLVFRRWLLHRRLMLWWWLLLWRLVFRRWLLHRRLVFRRWLLHRRLMLWWWLLLWRLVFRRWLLHRRLVFRRWLLHRRLMLWWWLLLWRLVFRRWLLHRRLVFRRWLLHRRLMLWWWLLHRRLMLWWWLLEWGMLRVISK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A6T3</accession>
    <name>A0AA39A6T3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_003991</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A6T3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21742">
        <property type="entry name" value="MobB_NDR_LATS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05599">
        <property type="entry name" value="STKc_NDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000042">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000106">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000290">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059233">
        <property type="entry name" value="MobB_NdrA/B/Cbk1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050839">
        <property type="entry name" value="Rho-assoc_Ser/Thr_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22988:SF76">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_135, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22988">
        <property type="entry name" value="MYOTONIC DYSTROPHY S/T KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="89"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="14">
        <location>
            <begin position="397"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="253"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="467"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9702036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="503" mass="57848" checksum="4DA164F6C8DBDAA9" modified="2024-01-24" version="1">MEEGSGEEEVLGSSLTMEKVAAAKQFIENHYRTQMKTIQERKERRWVLERRLASSDVPKEEQINLIKDLERKETEFMRLKRHKICVNDFELLTIIGRGAFGEVRLCREKKSGNIYAMKKLKKSEMLIRGQVEHVRAERNLLAEVASHCIVKLYYSFQDAEYLYLIMEYLPGGDMMTLLMREDTLSESVAKFYIAQSVLAIESIHKHNYIHRDIKPDNLLLDKNGHMKLSDFGLCKPLDCRTLSALKEDEVMDDENSRESMDIDGGFPDADNRSSWKSPREQLQHWQMNRRKLAFSTVGTPDYIAPEVLLKKGYGMECDWWSLGAIMYEMLVGYPPFYSDDPMTTCRKIVHWKNHLKFPEDPKLTAEAKDLIYRLLCDVEHRLGTGGVGQIKAHPWFKDVVWDKLYEMEAAFKPEVTGELDTRNFMKFDELDPPTQARSGSGPSRKMLLTPKDLSFVGYTYKNFDALKGLRPSSGDAGADYTARETADDTDVQMLAPSGDPMSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A6U6</accession>
    <name>A0AA39A6U6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GDT1 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_006311</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the GDT1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A6U6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015085">
        <property type="term" value="F:calcium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032468">
        <property type="term" value="P:Golgi calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032472">
        <property type="term" value="P:Golgi calcium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001727">
        <property type="entry name" value="GDT1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049555">
        <property type="entry name" value="GDT1-like_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12608:SF7">
        <property type="entry name" value="PROTEIN PAM71-HOMOLOG, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12608">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN HTP-1 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01169">
        <property type="entry name" value="GDT1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01214">
        <property type="entry name" value="UPF0016"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="102"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="130"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="171"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="301"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="333"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="68"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9700919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="354" mass="37995" checksum="6030B8AC147AC241" modified="2024-01-24" version="1">MQRQGQVLDTSIVSRRKLPLLALVDSFPCCAILSRRTIPSSLRCRDVSRLKSVCGTIRAQASNVGIGSGGYEGKEENDNQKIFVNGPPSDSSSNIEKPPDRIPYPLSIAIVLFGCALVFSLVTFTKGRPSSLLAAIAKSGFTAAFTLIFVSEIGDKTFFIAALLAMQYEKVLVLLGSMAALSLMTILSVVIGRIFHSVPAQFQTTLPIGEYAAVTLLMFFGLKSIKDAWDLPSNVVKSGDKSGPELDEFVEAEELVKEKVSKRLSNPLEIVWKSFSLVFFAEWGDRSMLATIALGAAQSPWGVASGAIAGHLFATSIAILGGAFLANYISEKLVGYLGGVLFLVFAVATFFGVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A7F4</accession>
    <name>A0AA39A7F4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_004152</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A7F4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22157">
        <property type="entry name" value="F-box_AtFBW1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011043">
        <property type="entry name" value="Gal_Oxase/kelch_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005174">
        <property type="entry name" value="KIB1-4_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050796">
        <property type="entry name" value="SCF_F-box_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31672">
        <property type="entry name" value="BNACNNG10540D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31672:SF7">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03478">
        <property type="entry name" value="Beta-prop_KIB1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50965">
        <property type="entry name" value="Galactose oxidase, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="17"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9702271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="407" mass="45985" checksum="1C564DE48C95A1FE" modified="2024-01-24" version="1">MQTDGGCCPPTASRKRKFPLDDLNQDLLERVLSWLPTSVFFRLTSVCKRWKSIADSATFQLACSQIPSRDPWFFMVDPHLNQWVVFDSAERNWKTLNHPPLLQLNPNINSIPVAAHGGLICFRNSSGHYIVCNPVTGSTRQLPPLDKPPQNQSLHAIAMNSFSTSHHSYKLVLVYGELSSLTFRIYNSSVNLWEEEIRLSRKAENPENSFQSDPDDDDETVYFLSITGNVVATNMQRSPSKQYSSVITRKDGGEEIVYFLSSSGTVVSCNLTSKLFYEYPKLLPALSEYSIDIVESKGELLVVVLSEFLETASLRVWKFDEDTRAWRRIAAMPPAMSHEFYGKKVDINCAAAGHQILICINSGELCRYILCDLVANEWIELPQCIKEGEAKEFMSAFSFEPRIEASV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A918</accession>
    <name>A0AA39A918_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ferredoxin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_004045</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A918"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34537">
        <property type="entry name" value="OS08G0459300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34537:SF1">
        <property type="entry name" value="OS08G0459300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041280308" description="Ferredoxin-like protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9702118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="222" mass="23557" checksum="BF1F59CC2EFB6DB3" modified="2024-01-24" version="1">MATTLWILSLSLVFLSVSAVTHKSVTDNPADELVALLNNNRTAHKESALYDNPGLACIALQYIKAYQGSCGEVGGEDAKRPADSQFAETFAPSCGVQVSTLTQITGRLLGCQTKYVSPAEAFSEILMKNSKSLEILYSKNHTELGGAVSGSDGGSPYFWCVLFSNGKSNSSFVLEGGVAKISKPGCFSGANDQCSSADDWSQTRRLWPYAAGLLIALGYAFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AFG7</accession>
    <name>A0AA39AFG7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">V-type proton ATPase subunit E</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_001596</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the V-ATPase E subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AFG7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033178">
        <property type="term" value="C:proton-transporting two-sector ATPase complex, catalytic domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2320.30">
        <property type="entry name" value="ATP synthase, E subunit, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00311">
        <property type="entry name" value="ATP_synth_E_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038495">
        <property type="entry name" value="ATPase_E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002842">
        <property type="entry name" value="ATPase_V1_Esu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45715">
        <property type="entry name" value="ATPASE H+-TRANSPORTING V1 SUBUNIT E1A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01991">
        <property type="entry name" value="vATP-synt_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160527">
        <property type="entry name" value="V-type ATPase subunit E-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9706107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="230" mass="26512" checksum="D8FF94086A6B447A" modified="2024-01-24" version="1">MNDADVSKQIQQMVRFIRQEAEEKANEISVSAEEEFNIEKLQLVEAEKKKIRQEYERKEKQVDIRKKIEYSMQLNASRIKVLQAQDDLVSSMKEAASKELLHVSHDHHVYKRLLRDLVVQSLLRLKEPAVLLRCRKDDLHLVESILDSAKDEYAEKANVYPPEIIVDHQVYLPPAPSHHQAHGPFCSGGVVLASRDGKIVCENTLDARLDVVFRKKLPEIRKWLFGQVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AFL5</accession>
    <name>A0AA39AFL5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_001944</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AFL5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11073">
        <property type="entry name" value="CYP76-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000007">
        <property type="entry name" value="Cytochrome P450 76C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47950">
        <property type="entry name" value="CYTOCHROME P450, FAMILY 76, SUBFAMILY C, POLYPEPTIDE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47950:SF4">
        <property type="entry name" value="GERANIOL 8-HYDROXYLASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7 8" id="KW-0349">Heme</keyword>
    <keyword evidence="5 7 8" id="KW-0408">Iron</keyword>
    <keyword evidence="3 7 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 8" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="7">
        <location>
            <position position="442"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9706716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="499" mass="56436" checksum="DFF65D13430E004C" modified="2024-01-24" version="1">MELLSCLLCFLAAWTSVYIMFSARRGRKHAAYKLPPGPVPLPIIGNLLNLGNRPHESLANLAKTYGPIMTLKLGYVTTIVISSAPMAKEVLQKQDLSFCNRSIPDAIRAANHNQLSMAWIPVSTTWRALRRTCNSHLFTSQKLDSNTHLRHHKVQELLASVEQSCRAGGPVDIGREAFRTSLNLLSNTIFSVDLVDPISETAQEFKELVRGVMEEAGKPNLVDYFPVLRQIDPQGIRRRLTIYFGRMIEIFDRMIKQRLQLRKIQGSIAISDVLDVLLNISEDNSNEIERSHMEHLLLDLFAAGTDTTSSTLEWAMAELLHNPEKLLKARMELLQTIGQDKQVKELDITRLPYVQAVVKETFRLHPAIPFLLPRRVEEDTDIEGFTVPKNAQVLVNAWAIGRDPNTWENPNSFVPERFLGLDMDVKGQNFELIPFGAGRRICPGLPLAIRMVHLMLASLIHSYDWKLEDGVTPENMNMEERYGISLQKAQPLQALPVRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AGX1</accession>
    <name>A0AA39AGX1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_002102</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AGX1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47941">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12854">
        <property type="entry name" value="PPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="130"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="165"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="200"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="235"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="272"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="307"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="342"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="414"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9706969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="480" mass="53958" checksum="4759816DA46E0677" modified="2024-01-24" version="1">MLICKSMAFSSVSRLLPYSIRHKNPNFSTALSPAEKYYTHLQKYGDNIEKTLPAVRAKLDSSCVNEVLNRCSLTQSQLGLRFFIWAGVQSYYRHSSYLYSKACELFRINQNPRAIIDVIEAYRVEGTVVSVKTFNVVLHLCREAKLADEALWILRKMAEFNIRADTVAYNSVIRLFCEKGDMDLAAGLMKEMGLIDLYPNMITYVTMIKGFCNVGRLEDACKLFKVMKGHGCSPNVVVYTVILDGVCRFGSLERALELLGEMEKESGDCSPNVVTYTSMIQSCCEKGKMMEALEILDRMRACGCAPNRVTVSILMKGFCAEGRVEEAFKLIDKVVAGGNVSYGECYSSLVASLVGNKNLQEAEKLFRRMLANALKPDGLACGTLIKALCLEGRVLDGFHLFDEFENMEGLSYLDSDIYSILLVGLSQKRHSVEAVKLARLMVDRGIQLKTPYFDSIVEHLKESGDKEIVMYLSRIFFDRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AJF0</accession>
    <name>A0AA39AJF0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4283 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_000644</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AJF0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025558">
        <property type="entry name" value="DUF4283"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34427">
        <property type="entry name" value="DUF4283 DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34427:SF5">
        <property type="entry name" value="DUF4283 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14111">
        <property type="entry name" value="DUF4283"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4283" evidence="2">
        <location>
            <begin position="65"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="253"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="285"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="385"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="296"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="314"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="387"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9708726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="407" mass="45506" checksum="82572EF52895B715" modified="2024-01-24" version="1">MGAHGGKIATNGRRFWEKINNQETRVVGKSVMEKSFVEVLKGSSWRPRNVVRVKVKREETLGILQKLGNCIVASWKPKTSGEEDLEKMGKLWPTSWGLTGNLGLARMEKNRVLLEFEKLEEARRVLDLEFWNPRSGCQKEGEESEEVWVKIVGLPVSMWNPTILRRVGEECGGFVAIDPQTKSLGELQWARILVKKRGDFLSSVLEMEIDKDVYTLSLWWELRPALKKNAEGCSEAFGWKRDEVRGDLELADATYGPLTPPTEGTGDQGSGLGWEKANRAQARERDLNITMGPGPHKSDPRLGPKEARRMDGPASSSSHLGSKSMQEGDVDVGLEEGPLFKRWAGQVDNTKLTEGLDRDGPNLLLSQAISIGFQKKDCCGSEMESLAARDSEDDRRRQEKCSFSTTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AJI0</accession>
    <name>A0AA39AJI0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_002409</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AJI0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47989">
        <property type="entry name" value="OS01G0750732 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47989:SF15">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE PBL7 ISOFORM X1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041311639" description="Protein kinase domain-containing protein" evidence="6">
        <location>
            <begin position="30"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="81"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="26"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9707384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="183" mass="20593" checksum="3E64CD70DE48C637" modified="2024-01-24" version="1">MLIASRTRNCFRFFLSSSYILRLVVLAKSNVVGHGGFGLVYRGVLHDGRKVAIKLMDRVGKQGEDEFKVEVELLSRFGSPYLLTLLGYTVQIITINCWFMSSWANLTLGPPPMSLYAKFVKQGLNLGGPVWFFSDILKDQSRKTRLPSPPTKFAGGSIGSFEAYKYVEDTLKWLLKFLIMAPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AL90</accession>
    <name>A0AA39AL90_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_000956</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AL90"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32343:SF26">
        <property type="entry name" value="RNA-BINDING (RRM_RBD_RNP MOTIFS) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32343">
        <property type="entry name" value="SERINE/ARGININE-RICH SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="2">
        <location>
            <begin position="6"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9709249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="246" mass="26453" checksum="EE74A2C72A9D29FA" modified="2024-01-24" version="1">MSTSGHTIEVTSLSPKVTEKDVYDFFAFSGAIERVEIVRSADECACTAYVTFKDAYAVETAVLLSGATIVDQRVCITRWGHYEDEFDLWNRPTWKLEDETSSTHALETNRSYPDAGEAVTMAQEVVKTMLAKGYILGKDALSKAKTFDESHQLSATAVAKVADLSQRIGLTDKFCAGVEAAKSVDQRYHVSEITKSAVSATGRTAAAAATTVVNSSYFSKGALWVSDALSRAAKAAADLGSHGVNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ALT4</accession>
    <name>A0AA39ALT4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Exostosin GT47 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_001479</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 47 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9710025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ALT4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010417">
        <property type="term" value="P:glucuronoxylan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009834">
        <property type="term" value="P:plant-type secondary cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004263">
        <property type="entry name" value="Exostosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040911">
        <property type="entry name" value="Exostosin_GT47"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11062:SF339">
        <property type="entry name" value="EXOSTOSIN GT47 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11062">
        <property type="entry name" value="EXOSTOSIN HEPARAN SULFATE GLYCOSYLTRANSFERASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03016">
        <property type="entry name" value="Exostosin_GT47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041356979" description="Exostosin GT47 domain-containing protein" evidence="6">
        <location>
            <begin position="19"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="Exostosin GT47" evidence="7">
        <location>
            <begin position="45"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9710025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="412" mass="46892" checksum="2457AF3E98458151" modified="2024-01-24" version="1">MGVWIWVLVFGFVWGIGAHGLDRHPHTERISGSAGDVLEDDPVGKLKVFVYELPSKYNKKILQKDPRCLNHMFAAEIFMHRFLLSSPVRTLNPEEADWFYTPIYTTCDLTPNGLPLPFKSPRMMRSAIQLISSNWPYWNRTEGADHFFVVPHDFGACFHYQEEKAIERGILPLLQRATLVQTFGQRNHVCLNEGSITIPPYAPPQKMQAHLIPQETPRSIFVYFRGLFYDVNNDPEGGYYARGARAAVWENFKDNPLFDISTEHPTTYYEDMQRAIFCLCPLGWAPWSPRLVEAVIFGCIPVIIADDIVLPFADAIPWEEIGVFVAEEDVPNLDTILTSIPPEVILRKQRLLANPSMKQAMLFPQPAQSGDAFHQILNGLARKLPHNKNVFLKHGEKALNWTAGPVGDLKPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AQ24</accession>
    <name>A0AA39AQ24_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_001171</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the amino acid/polyamine transporter 2 family. Amino acid/auxin permease (AAAP) (TC 2.A.18.2) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AQ24"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000033">
        <property type="entry name" value="Lysine histidine transporter 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48017">
        <property type="entry name" value="OS05G0424000 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="29"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="236"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="278"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="321"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="347"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="379"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="10">
        <location>
            <begin position="26"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="10">
        <location>
            <begin position="61"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9709559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="414" mass="46717" checksum="97A0861562B8FB49" modified="2024-01-24" version="1">MGHDQQEKDVRERAIDDWLPITSSRNAKWWYSAFHNVTTMVGAGVISLPYAMAELGWYMHEMVPGKQFDRYHELGQHAFGEKLGLWIVVPQQVIVEVGVDIAYMITGGKSLQKFHNTVCPNCKPIRTTYFIMIFASCHFLLSHLPNFNSITGVSFAASTISLTYSTIAWTASVHKGVQPDVQYTYTASTTTGRVFNFSALVNVAFAYAGHNVVLEIQATIPSTPEKPSKGPMWKGVIFAYIVVALCYFPVALIGYWMFGNSVADNILVTLEKPRWLIAAANLFVVIHVIGSYQIYPMPLFDMLEKLLVKKLHFTPCFRLRLITRTLYVAFTLFIAMLIPFFGSLHGFLGGLVFAPTTYFLPCIMWLAIHKPKRFSLSWFANWICIILGVILMILVPIGALRQIILQAKTFELFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D456</accession>
    <name>A0AA39D456_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">3-oxo-5-alpha-steroid 4-dehydrogenase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_025138</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the steroid 5-alpha reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D456"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1630:FF:000017">
        <property type="entry name" value="3-oxo-5-alpha-steroid 4-dehydrogenase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001104">
        <property type="entry name" value="3-oxo-5_a-steroid_4-DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039357">
        <property type="entry name" value="SRD5A/TECR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10556">
        <property type="entry name" value="3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10556:SF35">
        <property type="entry name" value="3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02544">
        <property type="entry name" value="Steroid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50244">
        <property type="entry name" value="S5A_REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="84"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="114"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="150"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="189"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="215"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="3-oxo-5-alpha-steroid 4-dehydrogenase C-terminal" evidence="7">
        <location>
            <begin position="145"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9671303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="259" mass="29333" checksum="C7C99722834F1036" modified="2024-01-24" version="1">MFLRFVFPPPPSLLVTAMSVVSFASMANAGLSELRGKHMEYSKFWEVKSGRKYKVDSRTGMVVCYTPAFLAGVASFALFPDESFRFLLLTSALSIHFFKRIFEVLFVHKYSGGMALDAAIVISLSYFISTATMIYAQHLSRDFPEPLMDLKYPGIALFLVGILGNLYHHCQLSKLRSKGEREYKIPKGGLFNLVVCPHYLFEILGFIGFSFISQTVYAFSLATGTALYLMGRSYSTRRWYVSKFENFPNNVKALIPYIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D523</accession>
    <name>A0AA39D523_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">cysteine desulfurase</fullName>
            <ecNumber evidence="3">2.8.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_026638</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.7" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D523"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031071">
        <property type="term" value="F:cysteine desulfurase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044571">
        <property type="term" value="P:[2Fe-2S] cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000003">
        <property type="entry name" value="Cysteine desulfurase IscS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000002">
        <property type="entry name" value="Cysteine desulfurase IscS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00331">
        <property type="entry name" value="Cys_desulf_IscS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010240">
        <property type="entry name" value="Cys_deSase_IscS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016454">
        <property type="entry name" value="Cysteine_dSase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02006">
        <property type="entry name" value="IscS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010611">
        <property type="entry name" value="PRK14012.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11601:SF34">
        <property type="entry name" value="CYSTEINE DESULFURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11601">
        <property type="entry name" value="CYSTEINE DESULFURYLASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005572">
        <property type="entry name" value="NifS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="9">
        <location>
            <begin position="57"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9670210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="454" mass="49856" checksum="0DF6238D005B3A37" modified="2024-01-24" version="1">MASRLHSSRLFQTLKTTSHTCRLRHLSTATAAAAKESYEDADGISMKGVKISGRPLYLDMQATSPVDPRVLDAMLPYYLSSYGNPHSRTHLYGWESDLAVEKARAQVAALINASPKEIVFTSGATESNNISVKGVMHFYKEKKRHVITTQTEHKCVLDSCRHLQQEGFEVTYLPVGSDGIVDIDLLRSAIRPDTGLVSVMTVNNEIGVIQPVEEIGNMCRELGVPFHTDAAQALGKIPVDVEKMNVSLMSLSGHKIYGPKGVGALYMRRRPRIRVEPQMNGGGQERGIRSGTVPTPLVVGMGAACELAMKEMEYDDKRISALQERLLNGIKAKLDGVVVNGSREKRYAGNLNLSFAYVEGESLLMGLKEVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEIDRAVELTVQQVEKLREMSPLYEMVKEGIDIKSIQWAQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D530</accession>
    <name>A0AA39D530_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycine-rich protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_025755</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D530"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041458427" description="Glycine-rich protein" evidence="1">
        <location>
            <begin position="26"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9672263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="122" mass="13196" checksum="4EB7E106546A15A0" modified="2024-01-24" version="1">MAFSKAFVLQGLLFAVLLILSKVSGRELAEAAQTQGSVEEANNWGHGYGHGRWGFGYGHGRRGFGHGHGHWGFGHGHGHRGHGHGHGHWGHGHEGIPRHNGAGGKVDFSILAYFLFIIFLLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D5A9</accession>
    <name>A0AA39D5A9_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_025408</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D5A9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041355654" evidence="2">
        <location>
            <begin position="26"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="139"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9671681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="169" mass="16658" checksum="5FAD5CF1C3D31D6E" modified="2024-01-24" version="1">MTWISISFSLWLILITPALVGGSSSKEPALVGGSTVYSSSKVAAVTARGPVPPSAPSSCTRIGVGNGDDRSCPPALVGGSTVYSSSKVAAVTAQGPVPPSAPSSCTRVGVGNGDDRRCPPAQVGGSTVYSSSKVAAVTARGPVPPSAPSSCTHTGVGNGDHRRCPPSTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D5J3</accession>
    <name>A0AA39D5J3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Ionotropic glutamate receptor C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_025290</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D5J3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015276">
        <property type="term" value="F:ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13686">
        <property type="entry name" value="GluR_Plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000172">
        <property type="entry name" value="Glutamate receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000188">
        <property type="entry name" value="Glutamate receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015683">
        <property type="entry name" value="Ionotropic_Glu_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001320">
        <property type="entry name" value="Iontro_rcpt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18966">
        <property type="entry name" value="IONOTROPIC GLUTAMATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00060">
        <property type="entry name" value="Lig_chan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00079">
        <property type="entry name" value="PBPe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="453"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="484"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="514"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor C-terminal" evidence="12">
        <location>
            <begin position="338"/>
            <end position="662"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9671514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="669" mass="74068" checksum="D23DADE9DCE73782" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D5M1</accession>
    <name>A0AA39D5M1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Haloacid dehalogenase-like hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_026593</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the HAD-like hydrolase superfamily. DOG/GPP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D5M1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07528">
        <property type="entry name" value="HAD_CbbY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000036">
        <property type="entry name" value="HAD family hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="entry name" value="Putative phosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044999">
        <property type="entry name" value="CbbY-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42896:SF4">
        <property type="entry name" value="OS08G0485900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42896">
        <property type="entry name" value="XYLULOSE-1,5-BISPHOSPHATE (XUBP) PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00413">
        <property type="entry name" value="HADHALOGNASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01135">
        <property type="entry name" value="C1.5.6:_HAD__Beta-PGM__Phospha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00035">
        <property type="entry name" value="phosphoglycolate_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00061496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9670132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="330" mass="36316" checksum="9BCA6BC9CC7EAB0D" modified="2024-01-24" version="1">MAGCCWMVSQHLLLSPPPNHVPLNFSSALISSTPLSFSLGSGSRSRVRVRASARARARARATRTSNALSVSASSTLQALIFDCDGVILESEDLHRRAYNDAFSHFNVRCPSSPSQHPLNWDSHFYDQLQNRIGGGKPKMRWYFKEYGWPSSTVFDTPPEDDGDRAKLIDILQDWKTERYKEIIKSGAVEPRPGVLRLMDETRAAGIKLAVCSAATKSSVILCLENLIGIERFQGLDCFLAGDDVKEKKPDPSIYQTAVKRLGVSEKDCLVVEDSVIGLQAATGAGMSCVITYTSSTADQDFKDAIAMYPDLSNVRLKDLELLLQNVVPAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D879</accession>
    <name>A0AA39D879_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2 NT-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_023360</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019448">
        <property type="entry name" value="NT-C2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34452">
        <property type="entry name" value="MYOSIN HEAVY CHAIN-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34452:SF1">
        <property type="entry name" value="SPORULATION-SPECIFIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10358">
        <property type="entry name" value="NT-C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51840">
        <property type="entry name" value="C2_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2 NT-type" evidence="3">
        <location>
            <begin position="6"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="156"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="523"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="601"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="720"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="877"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1062"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1350"/>
            <end position="1391"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1519"/>
            <end position="1581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="184"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="206"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="235"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9673750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1908" mass="216902" checksum="72A9AC7B1DDD57BE" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D888</accession>
    <name>A0AA39D888_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">glutathione transferase</fullName>
            <ecNumber evidence="1">2.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_026194</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the GST superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="1"/>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D888"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03185">
        <property type="entry name" value="GST_C_Tau"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03058">
        <property type="entry name" value="GST_N_Tau"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000018">
        <property type="entry name" value="Glutathione S-transferase U20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000014">
        <property type="entry name" value="Tau class glutathione S-transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045074">
        <property type="entry name" value="GST_C_Tau"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045073">
        <property type="entry name" value="Omega/Tau-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11260">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE, GST, SUPERFAMILY, GST DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11260:SF764">
        <property type="entry name" value="GLUTATHIONE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01152">
        <property type="entry name" value="Main.3:_Omega-_and_Tau-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="5">
        <location>
            <begin position="3"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="6">
        <location>
            <begin position="88"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9672847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="219" mass="25357" checksum="A2791B3CA5FDAB4A" modified="2024-01-24" version="1">MADEIISLNFWPSPFGMRVRVALAEKGLKYEYREEDLRNKSPLLLEMNPVHKKIPVLIHNGKPICESLIIVQYIDEVWKDKSPLLPSDPYQRAQARFWADYVEKKLFELGRKIWLTKGEEQETAKKEFIECLKLLEGELGEKPYFGGEKFGFVDVALVTLSCWFYAYESFGNFSIEAECPKLIAWTKRCMEKESVSSSLEDPHKVHGFAVGMRKKLGIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DA95</accession>
    <name>A0AA39DA95_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Auxin response factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_024061</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Auxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs).</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimers and heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ARF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DA95"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10017">
        <property type="entry name" value="B3_DNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.1040:FF:000001">
        <property type="entry name" value="Auxin response factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.330.10:FF:000001">
        <property type="entry name" value="Auxin response factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000047">
        <property type="entry name" value="Auxin response factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.330.10">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010525">
        <property type="entry name" value="ARF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044835">
        <property type="entry name" value="ARF_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033389">
        <property type="entry name" value="AUX/IAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003340">
        <property type="entry name" value="B3_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015300">
        <property type="entry name" value="DNA-bd_pseudobarrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31384">
        <property type="entry name" value="AUXIN RESPONSE FACTOR 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31384:SF10">
        <property type="entry name" value="AUXIN RESPONSE FACTOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06507">
        <property type="entry name" value="ARF_AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02309">
        <property type="entry name" value="AUX_IAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02362">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01019">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101936">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50863">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0927">Auxin signaling pathway</keyword>
    <keyword evidence="4 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TF-B3" evidence="10">
        <location>
            <begin position="148"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="PB1" evidence="11">
        <location>
            <begin position="839"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="482"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="556"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="799"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="508"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="572"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="809"/>
            <end position="818"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9674917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="949" mass="104581" checksum="F77C4C499B723224" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DAA9</accession>
    <name>A0AA39DAA9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_021999</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9676766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DAA9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd17039">
        <property type="entry name" value="Ubl_ubiquitin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47725">
        <property type="entry name" value="OS03G0364000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47725:SF2">
        <property type="entry name" value="UBIQUITIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041214934" description="Ubiquitin-like domain-containing protein" evidence="1">
        <location>
            <begin position="25"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="2">
        <location>
            <begin position="39"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9676766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="141" mass="16522" checksum="911D44C11BCF339D" modified="2024-01-24" version="1">MSSKTWDLFLLIHRFHLLMWKTLAKRSIRSGGLYRIVYMAMYIRVKRSKTTYFLQCDPTETILDVKQKLHTLIDQPINDQRLILVGTGEILDDSKTLADQKVENDAVVALTLRKDDNEFEDVNIVRPNDDFYQSRDPDGSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DAH3</accession>
    <name>A0AA39DAH3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_024191</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. PCMP-H subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DAH3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000333">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000344">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000345">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF484">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="160"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="224"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="286"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="321"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="387"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="422"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="14"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="14"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9675142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="576" mass="63888" checksum="663854C12FCF0E84" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DAU1</accession>
    <name>A0AA39DAU1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_022146</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DAU1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00030">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21669">
        <property type="entry name" value="SMP_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031468">
        <property type="entry name" value="SMP_LBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47264">
        <property type="entry name" value="OS01G0128800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47264:SF3">
        <property type="entry name" value="SYNAPTOTAGMIN-5 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51847">
        <property type="entry name" value="SMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="4" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SMP-LTD" evidence="7">
        <location>
            <begin position="92"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="6">
        <location>
            <begin position="465"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="6">
        <location>
            <begin position="584"/>
            <end position="699"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9677004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="815" mass="91966" checksum="8BE4E7E7231AD07D" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DC60</accession>
    <name>A0AA39DC60_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_020518</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DC60"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9678369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="100" mass="11456" checksum="0001E719968EDBB2" modified="2024-01-24" version="1">MSTHSRSRSSAISGEGYFDWHDNLERRQQENERKLRHENEVLWIQASSSSPSSNRRSRSLRAHPGLNEEVTYPENMDSPPNEYGARLGARPPVNSQYTNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DCA5</accession>
    <name>A0AA39DCA5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_020479</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DCA5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016709">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11075">
        <property type="entry name" value="CYP77_89"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000012">
        <property type="entry name" value="Cytochrome P450 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051103">
        <property type="entry name" value="Plant_metabolite_P450s"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24298:SF800">
        <property type="entry name" value="CYTOCHROME P450 89A2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24298">
        <property type="entry name" value="FLAVONOID 3'-MONOOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12 13" id="KW-0349">Heme</keyword>
    <keyword evidence="9 12 13" id="KW-0408">Iron</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 13" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="8 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="21"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="292"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="12">
        <location>
            <position position="501"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9678325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="557" mass="64198" checksum="ABDF8A544602688D" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DCN8</accession>
    <name>A0AA39DCN8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_021218</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DCN8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050942">
        <property type="entry name" value="F-box_BR-signaling"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005174">
        <property type="entry name" value="KIB1-4_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44259:SF107">
        <property type="entry name" value="F-BOX PROTEIN SKIP23-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44259">
        <property type="entry name" value="OS07G0183000 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03478">
        <property type="entry name" value="Beta-prop_KIB1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="4"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9679211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="448" mass="51289" checksum="464AD0FB323B53F8" modified="2024-01-24" version="1">MEADCDWAWLPKNLLHSILDQLQPMEDVIRFSAVCTEWRSVVVSNIGSRSLYQRQVPFLISRTGDNIGNLHSVTQKKMYKWRFPVPDNMKCCRCCGSSLGWLIMIDDTMEVILFNPFSGNTITFPPIRNLVFEKLCKLEYEDICSLLMQEEYGEEELEMAFDLEDDDEIQGEEEETHEEEGRQVWCEYRIIKGVLSADPGGSPDDYALMVIYGVEYKLAFIKSGDEDWTYIDNETSGFDYLFKTSPRVKGFRYFEDILYWKNLFYAVDGRGKLLTCDINTTLRVKLVDVPKGGFGINTTSSSTSFQQVVESLLGNETILSAFLVESVEGDLLWVSKFRFDLPTKKFCVCKIADNGGNVGWVKEEDLGDAALFLGDNHSISVRASDFPGCQSNSIYFLDREDEADCFDPGCPFDMGVYNLKHKSFARDYQLNPSERWMPPPIWILPTLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DCV4</accession>
    <name>A0AA39DCV4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_021120</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DCV4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000590">
        <property type="entry name" value="PR5-like receptor kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000178">
        <property type="entry name" value="serine/threonine-protein kinase PBS1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045874">
        <property type="entry name" value="LRK10/LRL21-25-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27009">
        <property type="entry name" value="RUST RESISTANCE KINASE LR10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="204"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9679089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="500" mass="56231" checksum="07E81E3D87668DF5" modified="2024-01-24" version="1">MRVVDVGLQKDNCSSLPLQSLMHGNFSHGDPYKLPASNSAVNFLECAAPVKSALYMDTALCSKNSSSNLSSSLSQTHSYVVVGDIRASDVEDSCSIGMVVWVSNSHLKINNSSFSGIHNGLLYGTELLWLRVCYLDDVHRKERVPCALNIAVRCCGIPCVFVFLIYKWRRRNLWMYSDIEEFIQSHNNLMPIRYSYSNIKKMTKNFKEKLGEGGFGSVFKGKLQSGRLVAVKMMINSKANGQDFINEVATIGRIHHVNVVQLIGFCAKGSKRALVFDFMPNGSLDRYIFPQTEANISLNFEKMYEISLGVAHGIEYLHRGCDMQILHFDIKPHNILLDKNFTPKVSDFGLAKLYPTDHSIVSVTAARGTMGYMAPELLYKNIGGVSYKADVYSFGMLLMEMAGRRKNLNVFAEHSSQMYFPSWVYDQFSEGKDIEMGDATEEEQKLAKKMIIVALWCIQLKPSDRPSMSKVVEMLEGNVELLQMPPKPFLTPQEVPAEDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DDM3</accession>
    <name>A0AA39DDM3_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_020531</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="5">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the carotenoid oxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DDM3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009570">
        <property type="term" value="C:chloroplast stroma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010436">
        <property type="term" value="F:carotenoid dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016121">
        <property type="term" value="P:carotene catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004294">
        <property type="entry name" value="Carotenoid_Oase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10543">
        <property type="entry name" value="BETA-CAROTENE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10543:SF58">
        <property type="entry name" value="CAROTENOID CLEAVAGE DIOXYGENASE 4, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03055">
        <property type="entry name" value="RPE65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="287"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="353"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="524"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR604294-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9678382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="537" mass="60037" checksum="ACA7589746763D61" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DG93</accession>
    <name>A0AA39DG93_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SGNH hydrolase-type esterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_016352</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9683813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DG93"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01838">
        <property type="entry name" value="Isoamyl_acetate_hydrolase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1110:FF:000002">
        <property type="entry name" value="isoamyl acetate-hydrolyzing esterase 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045136">
        <property type="entry name" value="Iah1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013830">
        <property type="entry name" value="SGNH_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14209:SF9">
        <property type="entry name" value="GDSL ESTERASE_LIPASE CPRD49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14209">
        <property type="entry name" value="ISOAMYL ACETATE-HYDROLYZING ESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13472">
        <property type="entry name" value="Lipase_GDSL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SGNH hydrolase-type esterase" evidence="4">
        <location>
            <begin position="83"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9683813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="328" mass="36363" checksum="8DCED39ECB19904C" modified="2024-01-24" version="1">MQIFKKFSQLSIFYSEYVSFPYPHATTVKKAHKCTINHCLLHSHLVSSHASHKFVSSGGKPAGGEGDGAGAEMVGPQRPQFVLFGSSIVQLSYANGGWGAILSDVYARKADIVLRGYYGWNSRRAVQVLHQVFPKDAATQPSLVIVYFGGNDSMGPHSSGLGPHVPLPEYIEHMRSIAVHLQSLSDTTRVIFLSCPPVNEAKVREGVSGIFSELVRTNELCRQYSEACIELCQEVGVKVVDLWTAFQKRDDWLTACFTDGVHLSAEGSEIVVEEILKVLKGAEWEPSLHWKSMLTEFGDDSPYDLVAFDGKTTLNPSDWTFHRAIQWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DL78</accession>
    <name>A0AA39DL78_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Non-lysosomal glucosylceramidase</fullName>
            <shortName evidence="1">NLGase</shortName>
            <ecNumber evidence="1">3.2.1.45</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_016213</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Non-lysosomal glucosylceramidase that catalyzes the hydrolysis of glucosylceramide (GlcCer) to free glucose and ceramide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a beta-D-glucosyl-(1&lt;-&gt;1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-glucose</text>
            <dbReference type="Rhea" id="RHEA:13269"/>
            <dbReference type="ChEBI" id="CHEBI:4167"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:22801"/>
            <dbReference type="ChEBI" id="CHEBI:52639"/>
            <dbReference type="EC" id="3.2.1.45"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the non-lysosomal glucosylceramidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.45" evidence="1"/>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DL78"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004348">
        <property type="term" value="F:glucosylceramidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006680">
        <property type="term" value="P:glucosylceramide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000006">
        <property type="entry name" value="Non-lysosomal glucosylceramidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014551">
        <property type="entry name" value="B_Glucosidase_GBA2-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006775">
        <property type="entry name" value="GH116_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024462">
        <property type="entry name" value="GH116_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052566">
        <property type="entry name" value="Non-lysos_glucosylceramidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12654">
        <property type="entry name" value="BILE ACID BETA-GLUCOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12654:SF3">
        <property type="entry name" value="NON-LYSOSOMAL GLUCOSYLCERAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04685">
        <property type="entry name" value="DUF608"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12215">
        <property type="entry name" value="Glyco_hydr_116N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028944">
        <property type="entry name" value="Beta_gluc_GBA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl-hydrolase family 116 N-terminal" evidence="4">
        <location>
            <begin position="93"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl-hydrolase family 116 catalytic region" evidence="3">
        <location>
            <begin position="527"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR028944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9687640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="949" mass="106284" checksum="D7C94DEE8AD4A5D7" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DM92</accession>
    <name>A0AA39DM92_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Histone-lysine N-methyltransferase ASHR3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_014640</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9689094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DM92"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042054">
        <property type="term" value="F:histone methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19175">
        <property type="entry name" value="SET_ASHR3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.270.10:FF:000043">
        <property type="entry name" value="Histone-lysine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000888">
        <property type="entry name" value="Histone-lysine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047893">
        <property type="entry name" value="ASHR3-like_SET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006560">
        <property type="entry name" value="AWS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025787">
        <property type="entry name" value="Hist-Lys_N-MeTrfase_SET2_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003616">
        <property type="entry name" value="Post-SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050777">
        <property type="entry name" value="SET2_Histone-Lys_MeTrsfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22884">
        <property type="entry name" value="SET DOMAIN PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00570">
        <property type="entry name" value="AWS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00508">
        <property type="entry name" value="PostSET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51215">
        <property type="entry name" value="AWS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50868">
        <property type="entry name" value="POST_SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51578">
        <property type="entry name" value="SAM_MT43_SET2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="AWS" evidence="13">
        <location>
            <begin position="285"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="SET" evidence="11">
        <location>
            <begin position="328"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="domain" description="Post-SET" evidence="12">
        <location>
            <begin position="451"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9689094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="494" mass="56065" checksum="DE063247DB9BDB3D" modified="2024-01-24" version="1">MPDLGNLLNSSSLTLSRCYNLKPLSDSCDSGGPLSPNSVNWEQRLRFPLFPKDEIECEKSGVGIRVLKRTRGGSLDRSKKLSNGKSLDDHVKAWADKKMESGATKSQCSLPFMSGASRLDECLVCHSFIYPGEEVSCTIEGCQGVYHLKCAKKELGFSTKRKFKCPQHACFVCKRRSYLRCVRCTIASHQKCAPWPEEMTLLRNRSGQAVCWRHPTDWRLDKKHAAPTSDIEEIFCRLPLPYDEEEFKIDLTWKDTVENKVEPTPYVHIRRNVYLVKKKRDDAADGIGCTNCSSVCSENCVCRVQCISCSKSCHCSENCTNRPFRKEKKIKIVKTELCGWGVEAAESINKGDFVIEYIGEVIDDALCERRLWDMKDRGDQNFYMCEIRKDFTIDATFKGNASRFLNHSCDPNCKLEKWQVEGETRVGVFAARSIKAGEPLTYDYRFVRFGPEVKCHCGAPSCHGYLGTKKKIAKVELCWGSKRKRSSCFALITV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DP57</accession>
    <name>A0AA39DP57_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_011158</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the peptidase S8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9691913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DP57"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02120">
        <property type="entry name" value="PA_subtilisin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04852">
        <property type="entry name" value="Peptidases_S8_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000006">
        <property type="entry name" value="Subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000005">
        <property type="entry name" value="subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.80:FF:000003">
        <property type="entry name" value="Subtilisin-like protease SBT1.9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034197">
        <property type="entry name" value="Peptidases_S8_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010259">
        <property type="entry name" value="S8pro/Inhibitor_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045051">
        <property type="entry name" value="SBT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041469">
        <property type="entry name" value="Subtilisin-like_FN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10795">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17766">
        <property type="entry name" value="fn3_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05922">
        <property type="entry name" value="Inhibitor_I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 10" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 10" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041283476" evidence="12">
        <location>
            <begin position="21"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="domain" description="Inhibitor I9" evidence="15">
        <location>
            <begin position="30"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="13">
        <location>
            <begin position="131"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="14">
        <location>
            <begin position="371"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="Subtilisin-like protease fibronectin type-III" evidence="16">
        <location>
            <begin position="655"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="192"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="195"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="9 10">
        <location>
            <position position="139"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="9 10">
        <location>
            <position position="211"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="9 10">
        <location>
            <position position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF05922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF17766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9691913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="768" mass="81935" checksum="ED8F6014DE55BD67" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DQ50</accession>
    <name>A0AA39DQ50_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_011232</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DQ50"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22160">
        <property type="entry name" value="F-box_AtFBL13-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053772">
        <property type="entry name" value="At1g61320/At1g61330-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053781">
        <property type="entry name" value="F-box_AtFBL13-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055357">
        <property type="entry name" value="LRR_At1g61320_AtMIF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34145:SF28">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34145">
        <property type="entry name" value="OS02G0105600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23622">
        <property type="entry name" value="LRR_At1g61320_AtMIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="5"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9692034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="520" mass="61002" checksum="7E0D511108DF68AE" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DQH8</accession>
    <name>A0AA39DQH8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase Ty1/copia-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_011211</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DQH8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47481">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47481:SF22">
        <property type="entry name" value="RETROTRANSPOSON GAG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase Ty1/copia-type" evidence="2">
        <location>
            <begin position="500"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="406"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="406"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9692005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="968" mass="107163" checksum="2D380F3154FA83C5" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DTD4</accession>
    <name>A0AA39DTD4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_008807</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DTD4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF78">
        <property type="entry name" value="OS12G0587133 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9696776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="619" mass="71347" checksum="CB2165D0675014C3" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DUI8</accession>
    <name>A0AA39DUI8_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_008698</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DUI8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008480">
        <property type="entry name" value="DUF761_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36378">
        <property type="entry name" value="COTTON FIBER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36378:SF1">
        <property type="entry name" value="COTTON FIBER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05553">
        <property type="entry name" value="DUF761"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="96"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9696609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="168" mass="18477" checksum="9A2A9A0D5F895978" modified="2024-01-24" version="1">MEDQAAMKAIVPLPTKKLSKKRGPMSLLKAALYMVRRNHSKKKAPVAVEVASNAMKLVGSMRPLHLQESNPPPPPCIHLSISMDNFQDVSPPPISPSAASSSGSSNDCMSRYASAQNLQELGRSDDDDDAVWGDGGDEMIDIKAKEFIAQFYEQMRLERLDSFKRATD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DXQ3</accession>
    <name>A0AA39DXQ3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Short-chain dehydrogenase/reductase</fullName>
            <ecNumber evidence="5">1.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_008084</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.-" evidence="5"/>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9699310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DXQ3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05324">
        <property type="entry name" value="carb_red_PTCR-like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045313">
        <property type="entry name" value="CBR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43490">
        <property type="entry name" value="(+)-NEOMENTHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43490:SF117">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE_REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0521">NADP</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU369024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9699310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="328" mass="35598" checksum="4748FEBAB59A7B4E" modified="2024-01-24" version="1">MLRSRISNKNLLIPLLIQKKMASTTSDSTTMRCAVVTGASKGIGLEICRQLASDGVMVVLTARDEKRGVEAVAKLHESSLSNVVFHQLDVMDANSITSLAKFIVTRYGKLDILVNNAGVIGAIVDWEAIATAIKTFKPEDGKNNVNVAELVNKGMKQTYELAEECVKTNYYGTKGVTEALFPCLLLSNSGRIVNVSSSLGSLKFVSNERVRMELNDVDVLSVETLDGIVNEYLNDVKEDMLHDKGWPTQTSAYTISKAAMNAYTRIVAKSYPSLLINCVCPGFIKTNMTSNTGFFTVEVGAKGPVMLALLPEGGPSGLFFQQMEASTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DZI3</accession>
    <name>A0AA39DZI3_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_006944</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DZI3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032440">
        <property type="term" value="F:2-alkenal reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08295">
        <property type="entry name" value="double_bond_reductase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000121">
        <property type="entry name" value="Prostaglandin reductase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041694">
        <property type="entry name" value="ADH_N_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045010">
        <property type="entry name" value="MDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43205:SF32">
        <property type="entry name" value="2-ALKENAL REDUCTASE (NADP(+)-DEPENDENT)-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43205">
        <property type="entry name" value="PROSTAGLANDIN REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16884">
        <property type="entry name" value="ADH_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxidoreductase N-terminal" evidence="3">
        <location>
            <begin position="10"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="2">
        <location>
            <begin position="169"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9701784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="347" mass="38600" checksum="5DA0A88D7541719B" modified="2024-01-24" version="1">MASGEEVSNKQVVLRDYVSGYLKESDMCMATATITLKVPEGNSKAVLVKNLYLSCDPYIRGHTKSIQGGYADEYFKPGSPIVGYGVAKVLDSGHPDFKKDDLVWGFTGWEEYSLITATESLFKIHHTDVPLSYYTGLLSMPGMTAYTGFYEVCAPKKGEYVFVSSAFGAVGQLVGQLAKLLGCYVVGSAGSKEKVDLLKTKFGFDEAFNYKEEKDYDACLKRYFPEGIDIYFDSVGGKMLDAALLNMRLHGRIAACGMISQYEFDQPEGVHNLFNIITKRVRVEGFMVYDYYHLYPKFLDTMIPYIKEGKIVYEEDITEGLESLPSALVRLFSGKNAGKAVVVVARE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E1F6</accession>
    <name>A0AA39E1F6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Non-specific serine/threonine protein kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_005130</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PI3/PI4-kinase family. TRA1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05163">
        <property type="entry name" value="PIKK_TRRAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1070.11:FF:000026">
        <property type="entry name" value="Phosphotransferases/inositol or phosphatidylinositol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000294">
        <property type="entry name" value="Transformation/transcription domain associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050517">
        <property type="entry name" value="DDR_Repair_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003152">
        <property type="entry name" value="FATC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003151">
        <property type="entry name" value="PIK-rel_kinase_FAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014009">
        <property type="entry name" value="PIK_FAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046807">
        <property type="entry name" value="Tra1_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046805">
        <property type="entry name" value="Tra1_ring"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139">
        <property type="entry name" value="ATAXIA TELANGIECTASIA MUTATED ATM -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139:SF1">
        <property type="entry name" value="TRANSFORMATION_TRANSCRIPTION DOMAIN-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02259">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20175">
        <property type="entry name" value="Tra1_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20206">
        <property type="entry name" value="Tra1_ring"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01343">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51189">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51190">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAT" evidence="4">
        <location>
            <begin position="2683"/>
            <end position="3226"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="3">
        <location>
            <begin position="3533"/>
            <end position="3860"/>
        </location>
    </feature>
    <feature type="domain" description="FATC" evidence="5">
        <location>
            <begin position="3869"/>
            <end position="3905"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2032"/>
            <end position="2058"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2310"/>
            <end position="2329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="3253"/>
            <end position="3334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="3277"/>
            <end position="3286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="3320"/>
            <end position="3333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9703704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="3905" mass="440595" checksum="3C695A3BE5935C17" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E3V6</accession>
    <name>A0AA39E3V6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase</fullName>
            <ecNumber evidence="3">3.2.2.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_002455</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>NAD(+) + H2O = ADP-D-ribose + nicotinamide + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16301"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57967"/>
            <dbReference type="EC" id="3.2.2.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:16302"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the disease resistance TIR-NB-LRR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.6" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E3V6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061809">
        <property type="term" value="F:NAD+ nucleosidase activity, cyclic ADP-ribose generating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050832">
        <property type="term" value="P:defense response to fungus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043068">
        <property type="term" value="P:positive regulation of programmed cell death"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10140:FF:000007">
        <property type="entry name" value="Disease resistance protein (TIR-NBS-LRR class)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32009:SF39">
        <property type="entry name" value="TIR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32009">
        <property type="entry name" value="TMV RESISTANCE PROTEIN N-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TIR" evidence="10">
        <location>
            <begin position="19"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9707438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="145" mass="16831" checksum="BF4FCB2E06348EF1" modified="2024-01-24" version="1">MASRSIQRPSSSSTSHSRWSYDVFLSFRSEDTRRSFTDHLYSALIRNNINTFRDDEEFPRGGEIAPELLKAIEESRIAIIVFSKTYAHSKWCLEELVKIMKSKEERKLTVIPIFYHVDPSKVRNQTGICGEAFTGHEENADEERK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E4P5</accession>
    <name>A0AA39E4P5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Matrin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_003161</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E4P5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000690">
        <property type="entry name" value="Matrin/U1-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041591">
        <property type="entry name" value="OCRE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013085">
        <property type="entry name" value="U1-CZ_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040023">
        <property type="entry name" value="WBP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13173">
        <property type="entry name" value="WW DOMAIN BINDING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13173:SF10">
        <property type="entry name" value="WW DOMAIN-BINDING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17780">
        <property type="entry name" value="OCRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06220">
        <property type="entry name" value="zf-U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50171">
        <property type="entry name" value="ZF_MATRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Matrin-type" evidence="8">
        <location>
            <begin position="11"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="149"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="37"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="166"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="217"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9704977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="257" mass="28586" checksum="7135F584D9927CFF" modified="2024-01-24" version="1">MTEYWVSQGNKWCDFCKIYIANNPSSIRNHELGQRHKDSVAKRLASMRKENAAKEKEQKEAAHALEQIESKAKRSYQKDIATFREARESNANALAQEDDSSTANDWECDSASGYYYSRSNGIYYDPNSGFYYSDAIGKWVTQEEAFATSQASSDSKQKGPVLKSPFSVSEGTQVIENKSAAKHQNGPPPGPVVSASLNPKRSSKCAPSSMAVNKRKRQDEKPKAVSKEEAAALKAREAAKKRVEEREKSLLGLYGSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E5C1</accession>
    <name>A0AA39E5C1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">3-beta hydroxysteroid dehydrogenase/isomerase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_002273</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the 3-beta-HSD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E5C1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006694">
        <property type="term" value="P:steroid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08958">
        <property type="entry name" value="FR_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000523">
        <property type="entry name" value="Cinnamoyl-CoA reductase-like SNL6 isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002225">
        <property type="entry name" value="3Beta_OHSteriod_DH/Estase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF623">
        <property type="entry name" value="CINNAMOYL-COA REDUCTASE-LIKE SNL6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01073">
        <property type="entry name" value="3Beta_HSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3-beta hydroxysteroid dehydrogenase/isomerase" evidence="4">
        <location>
            <begin position="17"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9707200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="308" mass="34146" checksum="2C6AA633B765E668" modified="2024-01-24" version="1">MAPDAISPHHTPPAVCVMDASGTVGSTLVGRLLLRGYTVHAAVQSHGEIQLLNGLSCDTQKLKIFHSDPFDYQSIMEALKGCSGLFYTFEPPPDQNSYDELMADVEVRAAHNVIEACAQTETIEKVVFTSSVTAVVWRDDRKSIPSDLDERHWTDINFSRKFKLWHAMSKTLAEKSAWALAMDRSVNMVSVNAGLLMSPDLTITHPYLKGAAEMYEDGVFVTVSVDFLVDAHICVFEDVSSYGRYLCFNHIINNNEDALKLAKMLAPSAPSPPQSCDQDMKMIQQRISNKKLNKLMVNFAGGPKDQMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ECK4</accession>
    <name>A0AA39ECK4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ABC1 atypical kinase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_001081</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. ADCK protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ECK4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd13971">
        <property type="entry name" value="ADCK2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004147">
        <property type="entry name" value="ABC1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044095">
        <property type="entry name" value="ADCK2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052402">
        <property type="entry name" value="ADCK_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45890:SF1">
        <property type="entry name" value="AARF DOMAIN CONTAINING KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45890">
        <property type="entry name" value="AARF DOMAIN CONTAINING KINASE 2 (PREDICTED)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03109">
        <property type="entry name" value="ABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="36"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="ABC1 atypical kinase-like" evidence="3">
        <location>
            <begin position="133"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9709432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="459" mass="52576" checksum="D46D4CA79C78785D" modified="2024-01-24" version="1">MFAFTFGELALAQRPLEYTNHYPLRNTLYMCAQDGHAYVTLLLFFFFFSVFEGVVLFLRALYLAILFSPCMVMAPFSDSFGHRFRNLWLQVVHGTLEKAATRLDLFARDLCTKLAELHTKAPEHSFSCTKKKKKKKKKKTIGKVFGRNLFEIFEDFEELPVASGSIAQVHQASLKFRYPGQQVKPVVVVVKVRHPGVGESIRRDFLIINLVAKISNFIPTLRWLRLDESVQQFAVFMMSQVDLAREAAHLSRFIYNFCLWKDVSFPKLVYPLVHLAVLYVDDLEGQKRIKSARAHIGTCALLKMLLVDNFIHADMHPGNILVQVAQSESSRKRLFKSKPHVIFLDVGMTAELSKSDHVNLLGFFKVVAQLDGRTAAECTLRLSKQQNCPNPKAFIKEVEKSFSFWGTPEVDLVHLAECMQQLLEKVRRHKVNIDGNVCTVTVTTLVLEDWQRKLDQDTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YHG3</accession>
    <name>A0AA38YHG3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ARM repeat N-terminal plant domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_026838</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YHG3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058868">
        <property type="entry name" value="ARM_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46578">
        <property type="entry name" value="ARM-REPEAT/TETRATRICOPEPTIDE REPEAT (TPR)-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46578:SF4">
        <property type="entry name" value="ARM-REPEAT_TETRATRICOPEPTIDE REPEAT (TPR)-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26524">
        <property type="entry name" value="ARM_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ARM repeat N-terminal plant" evidence="2">
        <location>
            <begin position="17"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="567"/>
            <end position="590"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9670546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="636" mass="72595" checksum="1A84425C303DE368" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YIM9</accession>
    <name>A0AA38YIM9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">PVL29_027223</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="22">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9671132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YIM9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000493">
        <property type="entry name" value="Probable LRR receptor-like serine/threonine-protein kinase At5g63710"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000015">
        <property type="entry name" value="Somatic embryogenesis receptor kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000016">
        <property type="entry name" value="Somatic embryogenesis receptor-like kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051824">
        <property type="entry name" value="LRR_Rcpt-Like_S/T_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48006">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN DDB_G0281931-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48006:SF102">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN DDB_G0281931-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="14 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="30"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="237"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="302"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAJ9671132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="624" mass="69949" checksum="F7F96AD89891C49B" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YIW8</accession>
    <name>A0AA38YIW8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Benzyl alcohol O-benzoyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_027321</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9671277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YIW8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050898">
        <property type="entry name" value="Plant_acyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31147">
        <property type="entry name" value="ACYL TRANSFERASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31147:SF66">
        <property type="entry name" value="OS05G0315700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9671277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="255" mass="28528" checksum="53D47C155CF1C42F" modified="2024-01-24" version="1">MSDAPGLIQFLNAISEMAQGLSMPSLLPIWPRELLNAQNPPRITHIHHEYEEVTNTKGTLMAMDENNLVHRSFFFGPKEIRALRNRLPASLGACSTFEVLIAYIWRCRTIAFAVDPDEVVRISCLINMRGKRGFDLPPGYYGNAFVCPASITKAGMLCKNPLEYAVRLLKKAKAKMSQEYMKSVADLLVIKGRPLFTQPGNYIVSDVTRAGFRDVDFGWGKLLASVCGSGTSKEEKGMLYLYACLRQSWKGLNRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YJT2</accession>
    <name>A0AA38YJT2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 15">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="11 15">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PVL29_025473</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 11 15">Belongs to the peptidase C19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="11 15"/>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YJT2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007154">
        <property type="term" value="P:cell communication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023052">
        <property type="term" value="P:signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02658">
        <property type="entry name" value="Peptidase_C19B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14295">
        <property type="entry name" value="UBA1_atUBP14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14388">
        <property type="entry name" value="UBA2_atUBP14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000086">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000103">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000026">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000371">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000099">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016652">
        <property type="entry name" value="Ubiquitinyl_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041432">
        <property type="entry name" value="UBP13_Znf-UBP_var"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001607">
        <property type="entry name" value="Znf_UBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF10">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00627">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02148">
        <property type="entry name" value="zf-UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17807">
        <property type="entry name" value="zf-UBP_var"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016308">
        <property type="entry name" value="UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00290">
        <property type="entry name" value="ZnF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50271">
        <property type="entry name" value="ZF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 11 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 11 15" id="KW-0645">Protease</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 11 15" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="7 11 15" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10 11 13" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBP-type" evidence="19">
        <location>
            <begin position="160"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="18">
        <location>
            <begin position="312"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="17">
        <location>
            <begin position="622"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="17">
        <location>
            <begin position="685"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="726"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="727"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="12">
        <location>
            <position position="321"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="772"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR016308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR016308-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR016308-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAJ9671779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="812" mass="90011" checksum="4C8509AA479CE936" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YK91</accession>
    <name>A0AA38YK91_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Chitin-inducible gibberellin-responsive protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_025554</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the GRAS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YK91"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005202">
        <property type="entry name" value="TF_GRAS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31636">
        <property type="entry name" value="OSJNBA0084A10.13 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03514">
        <property type="entry name" value="GRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50985">
        <property type="entry name" value="GRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="138"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Leucine repeat I (LRI)" evidence="3">
        <location>
            <begin position="212"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="VHIID" evidence="3">
        <location>
            <begin position="291"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Leucine repeat II (LRII)" evidence="3">
        <location>
            <begin position="372"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="SAW" evidence="3">
        <location>
            <begin position="510"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="VHIID" evidence="3">
        <location>
            <begin position="322"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9671960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="584" mass="65207" checksum="BDE78804C49A6A1C" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YMB8</accession>
    <name>A0AA38YMB8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_026405</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9673111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YMB8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035251">
        <property type="term" value="F:UDP-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000056">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050481">
        <property type="entry name" value="UDP-glycosyltransf_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48048">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48048:SF83">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9673111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="471" mass="52268" checksum="017519497568662F" modified="2024-01-24" version="1">MEMKAELVFIPIPEIGHFQSMLQLANHLVSRHHALSVTFLTFNDFVATAPPPPAASSLPGIRFITLPQVDLPHLDDVSGMDRYLLSLQLLKPHVKHAIQTHVLSSDSPQLAGLVLDPFALAMIDLATELGIASYIYFPSGAAMLEQVLRFPDLDSQVSELPATELTLPISVNPVPRRGLQTAMLEKDEDRYDPMLYLCRRLREAKGIIVNTLTELEPDLAELVSNRQYPPVYPVGPLIDPSDRTDDRIIKWLDDKPAGAVVFLCFGSRGALGAAQVQEIAHGLERSGYSFLWSLRQPPRVKHALPSDYTNPAEVLPDGFLDRTAEKGLVCGWTPQLKILSHPSIGGFISHGGWNSILESLWCGVPIMVWPMYAEQKLNACKIVRELGLGVGVTENEDYIDGRDLLMISTDGGDLVKREKLEIGVKRLMDGDNEVRRKVKQMSDTFREAVMDGGSSFVVLQQFIDDVFTEIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YMX1</accession>
    <name>A0AA38YMX1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8">Cytokinin riboside 5'-monophosphate phosphoribohydrolase</fullName>
            <ecNumber evidence="2 8">3.2.2.n1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_023132</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 8">Cytokinin-activating enzyme working in the direct activation pathway. Phosphoribohydrolase that converts inactive cytokinin nucleotides to the biologically active free-base forms.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>9-ribosyl-trans-zeatin 5'-phosphate + H2O = trans-zeatin + D-ribose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:48564"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16522"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:87947"/>
            <dbReference type="EC" id="3.2.2.n1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 8">
            <text>N(6)-(dimethylallyl)adenosine 5'-phosphate + H2O = N(6)-dimethylallyladenine + D-ribose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:48560"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17660"/>
            <dbReference type="ChEBI" id="CHEBI:57526"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="EC" id="3.2.2.n1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the LOG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.n1" evidence="2 8"/>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YMX1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016799">
        <property type="term" value="F:hydrolase activity, hydrolyzing N-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009691">
        <property type="term" value="P:cytokinin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.450:FF:000005">
        <property type="entry name" value="CASP-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005269">
        <property type="entry name" value="LOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031100">
        <property type="entry name" value="LOG_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00730">
        <property type="entry name" value="Rossman fold protein, TIGR00730 family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31223:SF50">
        <property type="entry name" value="CYTOKININ RIBOSIDE 5'-MONOPHOSPHATE PHOSPHORIBOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31223">
        <property type="entry name" value="LOG FAMILY PROTEIN YJL055W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03641">
        <property type="entry name" value="Lysine_decarbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102405">
        <property type="entry name" value="MCP/YpsA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0203">Cytokinin biosynthesis</keyword>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9673370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="219" mass="24279" checksum="F934CBB7207F0E34" modified="2024-01-24" version="1">MEETEMVKSRFKRVCVFCGSSTGKRNCYRDAAIELAQELVSRRLDLVYGGGSVGLMGLVSQEVHRGGGHVLGIIPKTLMCKEITGETVGEVRPVADMHQRKAEMARHSDCFIALPGGYGTLEELLEVITWAQLGIHDKPVGLLNVDGYYNYLLTFIDKAVDDGFIKPSQRHIIVSAPNARELVQKLEEYVPVHDGVVAKARWEAEQLELNASLQIDIAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YNV2</accession>
    <name>A0AA38YNV2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein DETOXIFICATION</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Multidrug and toxic compound extrusion protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_023463</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YNV2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13132">
        <property type="entry name" value="MATE_eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045069">
        <property type="entry name" value="MATE_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="79"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="123"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="156"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="189"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="214"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="342"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="382"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="411"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9673924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="495" mass="54024" checksum="B23980A4295DAE62" modified="2024-01-24" version="1">MGSVGGDEDLNRPLLQESKGVVLDPEKEKLASRVWIESKKLWHIVGPSIFSRIASYSMNVVTQAFAGHLGELELASISIANTVIVGFNFGLLLGMASALETLCGQAFGAKKYHMLGIYMQRSWIVLFLCCFLLLPFYVFATPLLKLLGQPDDVAELSGLVAICLIPLHFSFAFQFPLQRFLQSQLKTGVIAWVSLVAFVIHVFISWLFVYKLEFGIIGTAITLNFSWWILVFGMLGYTVCGGCPLSWTGFSMQAFSGLWEFVKLSTASGVMLCLENWYYRILILMTGNWKNAEVAVDALSVCMTINGWEMMIPLAFFAATGVRVANELGAGNGKGANFATKVSVATSSVIGVVFFLLIIVFQNQLAMIFTSSTAVLEQVDKLSFLLAITILLNSVQPVLSGVAVGSGWQAWVAYINLGCYYIIGLPLGIVMGWVLHLGVAVRSFPPLSGIAMHLGGWSVGCSGPNHAVGLDHWPFWVGSTLRFEWGKAPNPPPQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YP19</accession>
    <name>A0AA38YP19_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_023528</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YP19"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010048">
        <property type="term" value="P:vernalization response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15521">
        <property type="entry name" value="PHD_VIN3_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058585">
        <property type="entry name" value="Fn3_VIN3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032881">
        <property type="entry name" value="Oberon-like_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044514">
        <property type="entry name" value="VIN3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056990">
        <property type="entry name" value="VIN3-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46286:SF6">
        <property type="entry name" value="OS08G0220600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46286">
        <property type="entry name" value="VIN3-LIKE PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23376">
        <property type="entry name" value="Fn3_VIN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07227">
        <property type="entry name" value="PHD_Oberon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23380">
        <property type="entry name" value="VIN3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Oberon-like PHD finger" evidence="7">
        <location>
            <begin position="144"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="VIN3-like fibronectin type-III" evidence="8">
        <location>
            <begin position="347"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="VIN3-like C-terminal" evidence="9">
        <location>
            <begin position="528"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="100"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="100"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9674029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="615" mass="70241" checksum="341A4EF322754009" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YPH6</accession>
    <name>A0AA38YPH6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Syntaxin 6/10/61 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_023676</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Golgi apparatus</location>
            <location evidence="8">trans-Golgi network membrane</location>
            <topology evidence="8">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the syntaxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YPH6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048193">
        <property type="term" value="P:Golgi vesicle transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21442">
        <property type="entry name" value="SNARE_NTD_STX6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.90:FF:000004">
        <property type="entry name" value="Syntaxin 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015260">
        <property type="entry name" value="Syntaxin-6/10/61_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34949:SF6">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34949">
        <property type="entry name" value="OS05G0443700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09177">
        <property type="entry name" value="STX6_10_61_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Syntaxin 6/10/61 N-terminal" evidence="9">
        <location>
            <begin position="11"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF09177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9674265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="341" mass="38576" checksum="05D6DEC835BA4831" modified="2024-01-24" version="1">MGSDFERWEKDPFFSAAEEVQESADRMASTYRTWIHSTKEPSKMWNSEQLQRDLHTTLGTTKWQLEEFERAVGSSYEDSSADDARNRHRQFIGAIGNQVSTIESSLRESAASEGRSSIPWVRLDEGERDELALFLTGPALSQDKTTVKIMGMDNETVNPKEVDEESVPECPKNSCYLTEGGSLESREEKLTGHRRTASANADIGAWKIAVADDGVRQSLFNGQPYVPPRKVPSVSGLLCTMDCSSKLKWPKNGFRKLKVTDRHQEGDSAFLRPQISRGMSTCYEKGKSCLDSCDDCCDKQLYGWYGAMQRQLQRSQYQMQYSRPIQLTFWIALLCLIGVFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YPH7</accession>
    <name>A0AA38YPH7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cytochrome b5 heme-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_023678</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 8">Belongs to the cytochrome b5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YPH7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.120.10:FF:000002">
        <property type="entry name" value="Cytochrome b5 type B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050668">
        <property type="entry name" value="Cytochrome_b5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19359">
        <property type="entry name" value="CYTOCHROME B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19359:SF14">
        <property type="entry name" value="CYTOCHROME B5 A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00363">
        <property type="entry name" value="CYTOCHROMEB5"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-0349">Heme</keyword>
    <keyword evidence="5 8" id="KW-0408">Iron</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="117"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="9">
        <location>
            <begin position="4"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9674268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="147" mass="16348" checksum="F7CE63E9EB308855" modified="2024-01-24" version="1">MAPQKVFTLSQVAQHKSKTDCWFVIHGRVLDVTKFLEEHPGGEEVLVESAGKDATKDFEDIGHSKAAQNLLLKYQVGVLQGYNIQDGAAYTKDAPYKETKTKDMSAFVIKNDSKPTYAALLEFLVPLLVAGFFFGYRYLSKAAHLTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YRX5</accession>
    <name>A0AA38YRX5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Alginate lyase 2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_024470</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YRX5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014895">
        <property type="entry name" value="Alginate_lyase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33681:SF11">
        <property type="entry name" value="ALGINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33681">
        <property type="entry name" value="BINDING PROTEIN, PUTATIVE, EXPRESSED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08787">
        <property type="entry name" value="Alginate_lyase2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041319878" description="Alginate lyase 2 domain-containing protein" evidence="1">
        <location>
            <begin position="26"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="Alginate lyase 2" evidence="2">
        <location>
            <begin position="40"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9675561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="216" mass="24990" checksum="0393D01D5C0530BD" modified="2024-01-24" version="1">MKISHYYCITSLLLLILFCFSEGWCADPTYGFTAVPLTEWNFELQKPYDVPLEERYSYENGVRKLWVYADDKPHDPNSLTQPRTEVRIRGLDYSSGVWQFEGYAFVPNGTSGVTVSQIHGAAQDATTLILRIYNGDMRYYSADLIATGMYDRWFRLNVIHDVDGGKVTVFIDGVQKFQVKDKGPGDLYFKCGVYAAPRNISYYMESRWRDIKIYKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YTW5</accession>
    <name>A0AA38YTW5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_025134</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9676453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YTW5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9676453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="129" mass="14971" checksum="730E23687EEA9D01" modified="2024-01-24" version="1">MANRCFLCLSELETVDHLLLHCVKTRVLWNIVFSLFGVAWVLSCTVKETLLGWHGTFVGKTRKKAWKMAPLCIFWSVWKERNLLAFGNEELSLQRLKYSFVCNLWSWVRASIVLCPSSLISFLDWLGSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YU20</accession>
    <name>A0AA38YU20_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Aspartyl aminopeptidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_021953</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the peptidase M18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9676683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YU20"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05658">
        <property type="entry name" value="M18_DAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.250.10:FF:000001">
        <property type="entry name" value="Aspartyl aminopeptidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.250.10">
        <property type="entry name" value="Aminopeptidase i, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001948">
        <property type="entry name" value="Peptidase_M18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023358">
        <property type="entry name" value="Peptidase_M18_dom2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002759">
        <property type="entry name" value="PRK02813.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28570">
        <property type="entry name" value="ASPARTYL AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28570:SF16">
        <property type="entry name" value="ASPARTYL AMINOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02127">
        <property type="entry name" value="Peptidase_M18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00932">
        <property type="entry name" value="AMINO1PTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101821">
        <property type="entry name" value="Aminopeptidase/glucanase lid domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 9" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4 9" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="199"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="208"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9676683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="485" mass="53482" checksum="6C91C1DF4D8366E5" modified="2024-01-24" version="1">MAKEEQQIEGNDVVSDLINFLNSSPTAFHAVDEAKKLLKESGFVQVSERDNWNLEAGKRYFFTRNYSTIVAFAIGKKYVPGNGFCIVGAHTDSPCLKLKPISKVTKGGYLEVGVQTYGGGLWHTWFDRDLTVAGRVIIREGKDAFSHKLVRIEEPIMRIPNLAIHLDRGNNDGFKVNTHSHLVPILATAIKAELNKAVSENGPVDNGADEKKSNERTKTDNSRHHSLLLQLLATRLGCEPDDICDFELQACDTQPSTVAGAMKEFIFSGRLDNLCMSFCSLKAMIDATASESALEDETGVRMVALFDHEEIGSNSAQGAGSPVMFDALSQITNSFTSDSKLLQIAIQRSFLVSADMAHALHPNYMDKHEENHQPKLHGGLVIKHNANQRYATNAVTSFIFREIAAKHNLPVQDFVVRNDMPCGSTIGPILASGVGIRTVDVGAPQLSMHSIREMSAVDDVKHSYEHFRAYFQEFSCLDAKITVDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YWD8</accession>
    <name>A0AA38YWD8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Histone deacetylase</fullName>
            <ecNumber evidence="3 12">3.5.1.98</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PVL29_022686</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N(6)-acetyl-L-lysyl-[histone] + H2O = L-lysyl-[histone] + acetate</text>
            <dbReference type="Rhea" id="RHEA:58196"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="3.5.1.98"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11 12">Belongs to the histone deacetylase family. HD Type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.98" evidence="3 12"/>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YWD8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141221">
        <property type="term" value="F:histone deacetylase activity, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09991">
        <property type="entry name" value="HDAC_classI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.800.20:FF:000001">
        <property type="entry name" value="Histone deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003084">
        <property type="entry name" value="HDAC_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF10">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037913">
        <property type="entry name" value="His_deacetylse_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01271">
        <property type="entry name" value="HISDACETLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="8 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 12" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="17">
        <location>
            <begin position="38"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="388"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="422"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="437"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="276"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="315"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00061569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR037913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR037913-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR037913-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR037913-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ9677854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="507" mass="57660" checksum="DA3F9390ECFF11B5" modified="2024-01-24" version="1">MEDGIGGASLPSGPDAKKRRVCYFYEPTIGDYYYGQGHPMKPHRIRMAHNLVVHYALHRRMEINRPFPAGPDDIRRFHSDDYVDFLASVTPDSLHDHAHSRYLKRFNVGEDCPVFDGLFGFCQASAGGSIGAAVKLNRRDADIALNWAGGLHHAKKSEASGFCYVNDIVLGILELLKVHRRVLYVDIDIHHGDGVEEAFFTTDRVMTVSFHKFGDFFPGTGHIKDIGVGPGKYYALNVPLNDGMDDESFRGLFRPIIQKVMEVYQPDAVVLQCGADSLSGDRLGCFNLSVNGHADCLRFLRSFNVPLMVLGGGGYTIRNVARCWCYETAVAVGVEPDNKLPYNEYYEYFGPDYTLHIEPCNMENQNSPKDLEKIRNMLLEQLSRLPHAPSVPFQTTPPITKVPEEEEEDMDKRPKPRIWNGEDCESDPDEDEKPRAQFSNTNHHPTSNVEMRKTTYRVSQKLYSPMHLLPRVLYRSKLFLYVCDATPCKVEERFQKRLASRKRQYPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YWF5</accession>
    <name>A0AA38YWF5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Complex I assembly factor TIMMDC1, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Translocase of inner mitochondrial membrane domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_022641</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Tim17/Tim22/Tim23 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YWF5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055299">
        <property type="entry name" value="TIMMDC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13002">
        <property type="entry name" value="C3ORF1 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13002:SF1">
        <property type="entry name" value="COMPLEX I ASSEMBLY FACTOR TIMMDC1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02466">
        <property type="entry name" value="Tim17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="116"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="145"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00041344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9677773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="204" mass="21738" checksum="44A6B735EE8991A3" modified="2024-01-24" version="1">MEDVEKTEETVSNGTSKIPGTVNWGTATVLGVLAGMLYGGSKEASASVSKDAEVMLKLGSTPDKREQYRLMRDAMEKRFIRVTRGSIVGGVRLGMFTAAFYTLQNFFAEKRGVHDVFNIAGAGSLTAATFGLIMPGSIRWRARNVLLGSVLGAALGFPLGWAHLKLLETANEGRLAAYPDSDQIGQAKGGVGAAIDRLEGKLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YX11</accession>
    <name>A0AA38YX11_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_023005</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9678270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YX11"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004866">
        <property type="term" value="F:endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23375">
        <property type="entry name" value="beta-trefoil_STI_VvMLP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011065">
        <property type="entry name" value="Kunitz_inhibitor_STI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002160">
        <property type="entry name" value="Prot_inh_Kunz-lg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33107">
        <property type="entry name" value="KUNITZ TRYPSIN INHIBITOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33107:SF5">
        <property type="entry name" value="KUNITZ TRYPSIN INHIBITOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00197">
        <property type="entry name" value="Kunitz_legume"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00291">
        <property type="entry name" value="KUNITZINHBTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00452">
        <property type="entry name" value="STI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50386">
        <property type="entry name" value="STI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00283">
        <property type="entry name" value="SOYBEAN_KUNITZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041470766" evidence="1">
        <location>
            <begin position="28"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9678270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="204" mass="22378" checksum="1607505DA4BB43C6" modified="2024-01-24" version="1">MKTTSLPLFSLLLIALAFNPCPWVAEAASDPVLDIEGKELQSGVDYYILSVIHGRGGGLTMASVRNEICPLDVVQGEPEVSNGLPVMFTPVNPKEGVICVSTDHNIKFSAATICVQSTVWKLEYDESTGQWFITTGGDEGNPGHGTLSNWFKIEKCEENYKLVFCPTVCDFCKPVCGDIGIYIQDGHRRLALSDMPFKVMFHKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YXC2</accession>
    <name>A0AA38YXC2_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_020578</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YXC2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015495">
        <property type="entry name" value="Myb_TF_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47999:SF68">
        <property type="entry name" value="MYB DOMAIN PROTEIN 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47999">
        <property type="entry name" value="TRANSCRIPTION FACTOR MYB8-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH myb-type" evidence="5">
        <location>
            <begin position="3"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="4">
        <location>
            <begin position="3"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="4">
        <location>
            <begin position="56"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="5">
        <location>
            <begin position="60"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9678436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="159" mass="18837" checksum="F3476084F3136C88" modified="2024-01-24" version="1">MARPQEQRRPWTSEEDNMLKEFKLKYPNESWGKIAELAELDRTHKSCWERWNNHLKPGVNKENFSQEEDDTIIRIQQIKLHRNKWAFMAENHLPGRAANDIKNRWNNHLKKTHAMRSYLERHLLTDDETSDHSVPGDDLESLIVEYASLIPNVDPNANI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YY13</accession>
    <name>A0AA38YY13_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_020673</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the DESIGUAL family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YY13"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009606">
        <property type="entry name" value="DEAL/Modifying_wall_lignin1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052222">
        <property type="entry name" value="DESIGUAL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31769">
        <property type="entry name" value="OS07G0462200 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06749">
        <property type="entry name" value="DUF1218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="133"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="168"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="216"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="21"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="248"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="271"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9678568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="299" mass="31636" checksum="E7D04EEF2AB83451" modified="2024-01-24" version="1">MSSAVVAGTSSSCSAAFTRTHSIKPSLNPPPKPLTPSQSQKTTFQGLSLQDAKRGFSNSVLAADSRSSFASVRRGLEITARTAGAAKNIEVEVDKPLGLTLGQKSGGVIITGSQVQLSSSTASTYPRSPALPLGLTAAVALMIAQVMINIATGCICCRRGPHPSNSNWTLALICFVVSWFTFVIAFLLLLTGAALNDQHGEESMYFGNYYCYVVKPGVFAGAAVLSLASVTLGILYYLTLSSAKERNDPWPGPVAPNQAQGGGGIAMGQPQFPPQNPPNHPNQDPVFVHEDTYMRRQFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YYB3</accession>
    <name>A0AA38YYB3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_020823</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YYB3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025724">
        <property type="entry name" value="GAG-pre-integrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039537">
        <property type="entry name" value="Retrotran_Ty1/copia-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057670">
        <property type="entry name" value="SH3_retrovirus"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648">
        <property type="entry name" value="TRANSPOSASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648:SF28">
        <property type="entry name" value="TRANSPOSON-ENCODED PROTEIN WITH RIBONUCLEASE H-LIKE AND RETROVIRUS ZINC FINGER-LIKE DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13976">
        <property type="entry name" value="gag_pre-integrs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25597">
        <property type="entry name" value="SH3_retrovirus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="2">
        <location>
            <begin position="69"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="338"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9678747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="372" mass="42361" checksum="6B075B5823CA2E0E" modified="2024-01-24" version="1">MTSCPRDTIAVADTSTDTSLWHRRLGHMSEKGMKMLLSKGKLPKLKSIDFDMCESCILGKQKKVSFLKTGRTPKAKKLELVHTDLWGPSPVASLGGSRYYITFIDDSSRKVWVYFLKNKSDVFETFKKWKAMVETETGLRIKCLRSDNGGEYIDGGFSEYCAAQGIRMEKTIPGTPQQNGVAERMNRTLNERARSMRLHAGLPKTFWADAVSTAAYLINRGPSVPMEFRLPEEVWSGKEVKFSHLKVFGCVSYVHIDSDARSKLDAKSKICFFIGYGDEKFGYRFWDEQNRKIIRSRNVIFNEQVMYKDRSTVVSDATEIDHKKSEFVNLDELTESTVQKGGEEDKENVNSQVDLSTPVVEVRRSSRNIRPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z2E7</accession>
    <name>A0AA38Z2E7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">pyridoxal 5'-phosphate synthase</fullName>
            <ecNumber evidence="4">1.4.3.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_020154</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor metabolism; pyridoxal 5'-phosphate salvage; pyridoxal 5'-phosphate from pyridoxamine 5'-phosphate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor metabolism; pyridoxal 5'-phosphate salvage; pyridoxal 5'-phosphate from pyridoxine 5'-phosphate: step 1/1.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.5" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9681126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z2E7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004733">
        <property type="term" value="F:pyridoxamine phosphate oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008615">
        <property type="term" value="P:pyridoxine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000659">
        <property type="entry name" value="Pyridox_Oxase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024624">
        <property type="entry name" value="Pyridox_Oxase_Alr4036_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10851">
        <property type="entry name" value="PYRIDOXINE-5-PHOSPHATE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10851:SF3">
        <property type="entry name" value="PYRIDOXINE_PYRIDOXAMINE 5'-PHOSPHATE OXIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12766">
        <property type="entry name" value="Pyridox_oxase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0288">FMN</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyridoxamine 5'-phosphate oxidase Alr4036 family FMN-binding" evidence="8">
        <location>
            <begin position="30"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9681126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="161" mass="18226" checksum="08CE23D1C981E21F" modified="2024-01-24" version="1">MGTVVSWKQLLLRALDSNSSLKHSSFFQLIEELKHCPFAEICWYFTDSWEQFRINGRVDVIDGSDPDPMKLQERENAWFASSLKSRLQYLGPNPGLPSLSEQPAQEGSLDLSTGPVAAFCLLILDPDQVDYLNLKNNERLTFTSSKNVDGVKCWNSEKINP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z2G9</accession>
    <name>A0AA38Z2G9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">S-adenosyl-L-methionine-dependent methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_020117</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9681064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z2G9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37909">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT METHYLTRANSFERASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37909:SF1">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT METHYLTRANSFERASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13578">
        <property type="entry name" value="Methyltransf_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9681064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="311" mass="34478" checksum="BD2265D4E77E9BAF" modified="2024-01-24" version="1">MKEEQQHLHHHHHQQRKIITAKKLTRPTAYVLLLLLSYVLGYLSAPSSSSVHSPTAGTASIPTTAGAAVAESDLGHFSVTRRCGDPVPSKLVRQTILDRVFNGTSPFDSFPPQHVSHLLRPKRIKGWGSNGAVFENLIRRVKPRTIIEVGTFLGASAIHMAEVASQLGLKTQIICLDDFRGWPGFRDRFKDIAMINGDVMLLYQFMQNVIHVNATESVLPVPFSTASTLDKLCELGVLGDLIEVDAGHDFNSAWSDINRAYRILRPGGVIFGHDYFTSADNKGVRRAVNLFARLNKLRVQLDGQHWVIDSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z349</accession>
    <name>A0AA38Z349_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_020212</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclic nucleotide-gated cation channel (TC 1.A.1.5) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9681223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z349"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005216">
        <property type="term" value="F:monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.630">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45651:SF5">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-GATED ION CHANNEL 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45651">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-GATED ION CHANNEL 15-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0407">Ion channel</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="28"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="62"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="113"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="188"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="314"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="11">
        <location>
            <begin position="425"/>
            <end position="538"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9681223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="653" mass="75414" checksum="C59B511755D75D1D" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z5R3</accession>
    <name>A0AA38Z5R3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Putative plant transposon protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_018669</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z5R3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046796">
        <property type="entry name" value="Transposase_32_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20167">
        <property type="entry name" value="Transposase_32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Putative plant transposon protein" evidence="2">
        <location>
            <begin position="87"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="298"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="300"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="316"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9682789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="409" mass="46451" checksum="59882DC8AC3352B2" modified="2024-01-24" version="1">MAPKRAPAEPTSTGSKVKTREEAQMAGTKGKRSNPQRSSGSKQKQTEEALQPTQRFHLHFANRTVIPGRNIDFAKLSYFHFDVLFARMGWLPIVSVKEFLYPKVVKCFYSNMTFEDEGPITTTINGVQIVFDVAELCQILEIPNEGVCLYEAKKWLRVEGFKPVEAIQRLCGYPRSSRPTSHLLTVLSRILQHMISYIFIPKGGHRDDVSFLEAFLVDNILTERKVNIGYIIFQHMKACSISEDSVLPYGMFITKIVKYFNVNLRNETDGKKLKSFDTYDRASLRRMHFVRKKDGSWARKSSVPPSKVDVSSDNGSSEDEEYEDQDVEGRTSEKANVTRIPSTGGVRARADANHPSLIGPDDFKASDNLNARRLTSLENRIDGLHKEVKEGMQVIRGQHDEMIRPQLVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z5T6</accession>
    <name>A0AA38Z5T6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Germin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_018694</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May play a role in plant defense. Probably has no oxalate oxidase activity even if the active site is conserved.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Secreted</location>
            <location evidence="2 13">Extracellular space</location>
            <location evidence="2 13">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the germin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z5T6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02241">
        <property type="entry name" value="cupin_OxOx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000005">
        <property type="entry name" value="Germin-like protein subfamily 1 member 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006045">
        <property type="entry name" value="Cupin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001929">
        <property type="entry name" value="Germin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019780">
        <property type="entry name" value="Germin_Mn-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31238">
        <property type="entry name" value="GERMIN-LIKE PROTEIN SUBFAMILY 3 MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00190">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00325">
        <property type="entry name" value="GERMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00835">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00725">
        <property type="entry name" value="GERMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 13" id="KW-0052">Apoplast</keyword>
    <keyword evidence="7 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 10 13" id="KW-0464">Manganese</keyword>
    <keyword evidence="6 10 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0964">Secreted</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041483973" description="Germin-like protein" evidence="13">
        <location>
            <begin position="25"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="Cupin type-1" evidence="14">
        <location>
            <begin position="65"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="34"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU366015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9682819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="221" mass="23484" checksum="12CF9A819946EFAB" modified="2024-01-24" version="1">MKKMVINTLACIALLAMSFSLTSASDPSPLQDFCVAVNDTKTTVFVNGKVCKDPKVATPNDFFFSGLRVPGNTSNKVGSMVTPANVAQIPGLNTLGISLARVDFAPYGLNPPHTHPRATEILTVLEGTLSVGFVTSNPDNRLISKVLYKGDIFVFPEGLIHFQLNVGKTKAVAIAALSSQNPGVITIANAVFGSKPAISADVLTKAFQVDKKVVDYLQSQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z601</accession>
    <name>A0AA38Z601_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_018589</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z601"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039537">
        <property type="entry name" value="Retrotran_Ty1/copia-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648:SF31">
        <property type="entry name" value="RNA-DIRECTED DNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648">
        <property type="entry name" value="TRANSPOSASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="35"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9682693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="233" mass="26906" checksum="19971CA393CD5797" modified="2024-01-24" version="1">MFPHLFHGLDISEFHCETCELAKHTRVSFPISNKRSSHPLHLIHSDIWGLSTIPNVFGARWFVSLIDDCTKVTWIFLLKQKFDVSIVIPNFHSMVQNQFGVKIKSFRTDNARDYFNQLLSPYFQSQGILHDSSCVNTPQQNGVAERKNWHLLNTTRALLFQGSVPKSYWGEAVLTTTYMINRIPSRVLDNKSLVEILKSFYPHFKTSNGLTPRVFGCTTFVLVHSQYRDKLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z7L4</accession>
    <name>A0AA38Z7L4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_019337</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z7L4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.370:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 17.6-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000026">
        <property type="entry name" value="Transposon Ty3-G Gag-Pol polyprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005162">
        <property type="entry name" value="Retrotrans_gag_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041577">
        <property type="entry name" value="RT_RNaseH_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13975">
        <property type="entry name" value="gag-asp_proteas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03732">
        <property type="entry name" value="Retrotrans_gag"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17919">
        <property type="entry name" value="RT_RNaseH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0229">DNA integration</keyword>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="10">
        <location>
            <begin position="654"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="313"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="334"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9683720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1162" mass="132963" checksum="18B85E5D8BC8FB16" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z7V1</accession>
    <name>A0AA38Z7V1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_019187</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Functions as an E3 ubiquitin ligase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z7V1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000578">
        <property type="entry name" value="RING-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000565">
        <property type="entry name" value="RING-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058678">
        <property type="entry name" value="ARM_PUB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23315">
        <property type="entry name" value="U BOX DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23315:SF339">
        <property type="entry name" value="U-BOX DOMAIN-CONTAINING PROTEIN 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25598">
        <property type="entry name" value="ARM_PUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04564">
        <property type="entry name" value="U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51698">
        <property type="entry name" value="U_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="U-box" evidence="10">
        <location>
            <begin position="27"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="8">
        <location>
            <begin position="288"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="8">
        <location>
            <begin position="329"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="166"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="166"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9683504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="519" mass="56507" checksum="364C006985657BB3" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z934</accession>
    <name>A0AA38Z934_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Scarecrow-like protein 9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_016660</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the GRAS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z934"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005202">
        <property type="entry name" value="TF_GRAS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31636">
        <property type="entry name" value="OSJNBA0084A10.13 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03514">
        <property type="entry name" value="GRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50985">
        <property type="entry name" value="GRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="353"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Leucine repeat I (LRI)" evidence="3">
        <location>
            <begin position="387"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="VHIID" evidence="3">
        <location>
            <begin position="465"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Leucine repeat II (LRII)" evidence="3">
        <location>
            <begin position="546"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="SAW" evidence="3">
        <location>
            <begin position="684"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="VHIID" evidence="3">
        <location>
            <begin position="496"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="129"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="353"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9684283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="762" mass="85268" checksum="B1C674ADF0D74796" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZAY2</accession>
    <name>A0AA38ZAY2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NAD-dependent epimerase/dehydratase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_017319</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZAY2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08958">
        <property type="entry name" value="FR_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000085">
        <property type="entry name" value="Dihydroflavonol reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF575">
        <property type="entry name" value="NAD-DEPENDENT EPIMERASE_DEHYDRATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="4">
        <location>
            <begin position="10"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9685242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="270" mass="30070" checksum="D386DA87827C4D9E" modified="2024-01-24" version="1">MSVAVAEKVVCVTEGAGYIASWLVKLLLLRGYTVKATLRNPAKERLRLFKANLLEEGSFDSIVEGCEGVFHTASPVLLEVTYPKVELIDPAVKGTLNVLRSCAKVLAIRRMVVTSSIVAVVYNGKPLTSDVIVDETWFSDPAFCEESKLCYVLSKTLVEEAAWKFAREHGIDLVTMNPGVMIGPLLQPTINLTMEIILNMINAFEISSASGRYCMVERVTYRFEAIKILHELYPASTFLRMKSLAIDFIPLEVSLKDTIESLKEKNFINL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZBA6</accession>
    <name>A0AA38ZBA6_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_017432</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZBA6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010333">
        <property type="term" value="F:terpene synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016102">
        <property type="term" value="P:diterpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00684">
        <property type="entry name" value="Terpene_cyclase_plant_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.600.10:FF:000007">
        <property type="entry name" value="Isoprene synthase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.130">
        <property type="entry name" value="Terpene synthase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034741">
        <property type="entry name" value="Terpene_cyclase-like_1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044814">
        <property type="entry name" value="Terpene_cyclase_plant_C1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001906">
        <property type="entry name" value="Terpene_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036965">
        <property type="entry name" value="Terpene_synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050148">
        <property type="entry name" value="Terpene_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005630">
        <property type="entry name" value="Terpene_synthase_metal-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225:SF244">
        <property type="entry name" value="1,8-CINEOLE SYNTHASE 1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225">
        <property type="entry name" value="OS04G0344100 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01397">
        <property type="entry name" value="Terpene_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03936">
        <property type="entry name" value="Terpene_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01019">
        <property type="entry name" value="Terpene_Cyclase_Like_1_C_Termi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01604">
        <property type="entry name" value="Terpene_Cyclase_Like_1_C_Termi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Terpene synthase N-terminal" evidence="3">
        <location>
            <begin position="61"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="Terpene synthase metal-binding" evidence="4">
        <location>
            <begin position="276"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9685393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="571" mass="66765" checksum="744FD7AA24D7DBDC" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZD20</accession>
    <name>A0AA38ZD20_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">3,9-dihydroxypterocarpan 6A-monooxygenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_015501</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZD20"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016709">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051103">
        <property type="entry name" value="Plant_metabolite_P450s"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24298:SF204">
        <property type="entry name" value="CYTOCHROME P450, FAMILY 712, SUBFAMILY A, POLYPEPTIDE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24298">
        <property type="entry name" value="FLAVONOID 3'-MONOOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0349">Heme</keyword>
    <keyword evidence="7 8" id="KW-0408">Iron</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 7 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="7">
        <location>
            <position position="420"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9686657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="488" mass="55011" checksum="F4E1865C66E5DE99" modified="2024-01-24" version="1">MDTDTTFYFVPYILCIIPSVLLLSFLKTLVKPPTASRPPPSPPTLPIIGHVHLLTSTLPKCFQTLASRYGPLMQLRLFSSTCIVVSNAAVAKEVMKTHELAFASRPEFGSSEHFIYKGSKFIMAEYGPYWRFMKKLCLTKLLAAPTLDRLIHIREEEMEKLMDTLIQRSRKGEASDLSKELTTLTSNIICRMAMSTRCSRNGNEAEEMMELVKGVVELGGKLSIGDALGALGRLDLFGYGKKLEAKLRKFDNPSAEWKLTRTDIKSFFLDILLAGTDTQSVATQWAMAELINRPRVFHKLREEIDLIVGSTRMVKESDFPNLPYLQAVVKETLRLHTSAPFILRQCIQDCKIDGYDIKANTRVMISAFAIMQDPNSWEDPNEFIPERFWVNSGEKTVDHVMEMKGQDFRYVPFGSGRRGCPGAALASMVMQMTIGRLVQCFDWRVKDGEKADMNVGPGFSAEMKTPLVCYTSIHFDPFGATRAKHVEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZDW6</accession>
    <name>A0AA38ZDW6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycosyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_015920</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="5"/>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZDW6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035251">
        <property type="term" value="F:UDP-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000056">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000080">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050481">
        <property type="entry name" value="UDP-glycosyltransf_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48048">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48048:SF45">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9687243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="475" mass="52900" checksum="C8224C4894F1430B" modified="2024-01-24" version="1">MEQTELVFIPFPVIGHLASALEIAKLITKRDPRFSITIFIMKFPFGSTDAMDTDSDSIRFVTLPPVEVSSETTPSGHFFSEFLKVHIPVVRDAVHELTRSNSVRFSGFVIDMFCTHMIDVADEFGVPSYLFFSSGAAVLGFLLHVQFLHDYEGLDINEFKDSDAELDVPTFVNSVPGKVFPAWMFDKESGGAEMLLYHTRRFREVKGILVNTFIELESHAIQSLSGSTVPKVYPVGPILNTRMGSGGGQQDASAIMSWLDDQPPSSVIFLCFGSMGSFGADQIKEIAYGLEHSGHRFLWSLRQPPPKGKMEFPGGYENIEEVLPEGFLHRTARIGKVIGWAPQIAVLAHSAVGGFVSHCGWNSLLESLWYGVPVATWPIYAEQQINAFQMVKGLGLAVEIKIDYNEDNDYVVSAHEIENGLRNLMNINSEVRKKRKEMEKISRKVMIDGGSSHFSLGHFIEDMMDSKVMKGKDAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZF43</accession>
    <name>A0AA38ZF43_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Kinetochore protein Nuf2 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_016068</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
            <location evidence="1">Centromere</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NUF2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZF43"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031262">
        <property type="term" value="C:Ndc80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.60">
        <property type="entry name" value="Ncd80 complex, Nuf2 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005549">
        <property type="entry name" value="Kinetochore_Nuf2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038275">
        <property type="entry name" value="Nuf2_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48441">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48441:SF1">
        <property type="entry name" value="NT-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03800">
        <property type="entry name" value="Nuf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="8" id="KW-0137">Centromere</keyword>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinetochore protein Nuf2 N-terminal" evidence="10">
        <location>
            <begin position="4"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="141"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="298"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9687432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="453" mass="52416" checksum="D929FD7B92F5AF90" modified="2024-01-24" version="1">MSKFEYPMIPRSEIIAILNESKIAAVYDEDLINPNPDFVSDLYTRLLIHLDSLQEDPGQVEFAALERLENPDWHRDSVRIMNLYSKIKELVASLDCPWKFTLKDLIRPDRDRTEKFLGTILNFFLHKDSKMNELRPIVEESNLLDEERKQLEDRISQLDAEITDYNEAREREMPLVQEIEVKVKELRQAIPSLNNQQMSLRTSFRKMKEKVAEMDEKISSAEFALVQSVQENANLRSKIVQSPDKLQRTLEEKKSVRVEAKNAERSAMQSFQEKSAIVEVYTKAGKKMSKHFAQMQAIQEQVNSAKAIEKDLKVLKTKLSEEGVLDKSFEAKLLERQGKVEQLDELRKQLEKERNFKCEEAAKELNNVKLAVESKRRELEARQRKVEAEAAEVDATTLKINSVKESGAAKQQRLARKGEEIVKEFHQYANSIGVLLPRIQVEPEVDQDMGRSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZFB3</accession>
    <name>A0AA38ZFB3_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_013982</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZFB3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9688023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="149" mass="17633" checksum="47F4ED83F0A25F67" modified="2024-01-24" version="1">MAYYNPYQYLEYNPADGCPQFWTYKKVSFVASLSLIFLLITVSLRPWTFVFISMVAASLTMSFDYEGAFMDYFWRLITIVTLVFLFWFLIWCSQKVRRPRSRSNEVEDDDEDDESISVYVSMGLIAILALVLTWCVRKLQRPRRRRGGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZFU4</accession>
    <name>A0AA38ZFU4_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_016144</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZFU4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000026">
        <property type="entry name" value="Cytochrome P450 82C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050651">
        <property type="entry name" value="Plant_Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47947">
        <property type="entry name" value="CYTOCHROME P450 82C3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47947:SF1">
        <property type="entry name" value="CYTOCHROME P450 82E3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11 12" id="KW-0349">Heme</keyword>
    <keyword evidence="8 11 12" id="KW-0408">Iron</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 12" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="11">
        <location>
            <position position="433"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9687539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="493" mass="55337" checksum="3E97432503797B6C" modified="2024-01-24" version="1">MDLPSHFLAIAGLILGLGLWYNHWRGKTLTHKSKGMSPPEPSGAWPFVGHLHLLQGKVPVFRTVGAMADKVGPVFVIRLGMYRALVVSNHEAAKECFTTYDKGPLWREMRKLSMLEILSTRRLSDLMHVQVSELHAGIKDLYILGKDYNWVNPKKVVISEWFEHLNFNVVLRMIAGKRYFNNVVHGGEEAGSATAAIKKLVPPAGAFVASDLIPFLEWVDLQGHLSSMKQVAKEMDSVLESWVEEHTGRFNTEASSRQDFIDIMLTKLKDACLFGYSRDTIIKATVLTLIVAGSDTISITSTWLLSALLNNRHVMKHAQEELDLKVGRDRWVEQSDIQNLVYLKAIVKETLRLYPAIPLLVPHEAMEDCHVGGYHFPKGTGLLVNAWKLHRDPAVWSNPEEFQPERFLTSHATVDVFGQHFELIPFGSGRRSCPGINMALQMLHLTTAQLLQGFDMATPSNSPVDMAEGISITMPKFTPLEVMLTPRLPAELY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZIT8</accession>
    <name>A0AA38ZIT8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ubiquitin-specific protease family C19-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_012500</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9689868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZIT8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040339">
        <property type="entry name" value="At1g16860-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33709">
        <property type="entry name" value="OSJNBA0035M09.9 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33709:SF17">
        <property type="entry name" value="UBIQUITIN-SPECIFIC PROTEASE FAMILY C19-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="167"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="194"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="72"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="114"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9689868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="432" mass="46541" checksum="B32D195C60600A29" modified="2024-01-24" version="1">MGSRIPSHQLSNGLYVSGRPEQHKERQPTIGSRAVPYTGGDVKKSGELGKMFDIAVVDPSAGGPPPLKPSRHSSSSQHTSGSVRSGPNSGQLPKLSNSGPIQKKSSGPMPLQPTGLITSGPLGSSGSIGGRRSGQLEPSGSMGKAVYGSSVTSLNEEAKFGFKVSKAVMWVFLVVVAMGLLVGAFLMVAVKKAVILVAVAGILVPVIMLVLWNYAWGKRGLLGFLKRYPDAELRGAIDGQYVKVTGVVTCGSIPLESSYQRIPRCVYVSTELYEYRGWCGKSANPKHRFFSWGSRHSEKHVADFYISDFQSGLRAMVKAGYGAKVAAFVKPATVVNVTKEKRELSPSFLRWLADRNLSSDDRIMRLKEGYIKEGSTVSVMGVVRRHDNVLMIIPPAEPVSTGCQWARCLLPSYVEGLVLMCDDNQNADVVPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZK62</accession>
    <name>A0AA38ZK62_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Glutamate receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_012970</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Glutamate-gated receptor that probably acts as a non-selective cation channel. May be involved in light-signal transduction and calcium homeostasis via the regulation of calcium influx into cells.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">May form heteromers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZK62"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015276">
        <property type="term" value="F:ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19990">
        <property type="entry name" value="PBP1_GABAb_receptor_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000172">
        <property type="entry name" value="Glutamate receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000477">
        <property type="entry name" value="Glutamate receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000188">
        <property type="entry name" value="Glutamate receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044440">
        <property type="entry name" value="GABAb_receptor_plant_PBP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015683">
        <property type="entry name" value="Ionotropic_Glu_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001320">
        <property type="entry name" value="Iontro_rcpt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34836">
        <property type="entry name" value="OS06G0188250 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34836:SF1">
        <property type="entry name" value="OS09G0428600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00060">
        <property type="entry name" value="Lig_chan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0407">Ion channel</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="7 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="490"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="551"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="688"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="domain" description="Receptor ligand binding region" evidence="16">
        <location>
            <begin position="1"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor C-terminal" evidence="15">
        <location>
            <begin position="488"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="749"/>
            <end position="819"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9690586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="19">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="819" mass="92619" checksum="A6C9FB3B39660F7B" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZN92</accession>
    <name>A0AA38ZN92_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Acid phosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_011339</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZN92"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005519">
        <property type="entry name" value="Acid_phosphat_B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31284">
        <property type="entry name" value="ACID PHOSPHATASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31284:SF19">
        <property type="entry name" value="VEGETATIVE STORAGE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03767">
        <property type="entry name" value="Acid_phosphat_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041464040" description="Acid phosphatase" evidence="2">
        <location>
            <begin position="22"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9692221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="224" mass="25196" checksum="D292E9CE331E9C63" modified="2024-01-24" version="1">MGGVLLLWLFLFAGAVELSLGISHEIHLLRPQLGSNGHHVPGLSCLSWRLDYVGHYMLGHQNRKDSRAVVYEALTYAQSLKLAGDGKDIWVFDVDETSLSNLPYYAKHGFGYLNLGKAPALPESLKLYKKLLSLRIKAVFITGRPEAQRNVTAANLRNVGYHTWERLILKGSSTGIAIVYKSNERKKLEKSGYRTIANIGDQWSDILGTNIGNWTFKLPNPMWW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZP25</accession>
    <name>A0AA38ZP25_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NPH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_011539</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the NPH3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZP25"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043454">
        <property type="entry name" value="NPH3/RPT2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027356">
        <property type="entry name" value="NPH3_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32370">
        <property type="entry name" value="OS12G0117600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03000">
        <property type="entry name" value="NPH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51649">
        <property type="entry name" value="NPH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="NPH3" evidence="4">
        <location>
            <begin position="22"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="327"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9692537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="431" mass="48265" checksum="8856E1737AF925B0" modified="2024-01-24" version="1">MKKLAVPESITLAGKRPVFAAECWLDDACILDMDYFVKTLSGIKAKGVRPDLIGSIIAHYASKWLPDLLTDDEKGLTIFEESPESATASWMKKRFFVETLVGILPPEKDSIPCNFLLRLLRTANMVGAEPTYRAELESRISWQLDQASLKELMIPSFCHTCATLLDVELVMRLVKRFVNMDDAAKSGAALIKVAKLIDSYLAEAAVDSNLTLQEFASLASALPSHARSTDDGLYRAIDTYLKAHPGVSKQDRKVLWRIIDSRKLSPEASFHAAHNERLPVRAVIQVLLSEQTKLTRQLDWSGSFSGTRSPNPGVLEHPSRCHSKREISAQQAEIRRLREEVRRLQGQCSAMQGQMERLLEKKKGLFRWKKLVMPSFKSTGVDAGSIGEKIQEREEGEGEAAFGQETPLDFKITKTKLVRGKTPPKWRNSLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZP36</accession>
    <name>A0AA38ZP36_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Plasma membrane ATPase</fullName>
            <ecNumber evidence="16">7.1.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PVL29_011082</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15 16">
            <text>ATP + H2O + H(+)(in) = ADP + phosphate + 2 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:20852"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Cell membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIIA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.1" evidence="16"/>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9691784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZP36"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008553">
        <property type="term" value="F:P-type proton-exporting transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120029">
        <property type="term" value="P:proton export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02076">
        <property type="entry name" value="P-type_ATPase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000045">
        <property type="entry name" value="ATPase 4 plasma membrane-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000004">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000004">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000211">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006534">
        <property type="entry name" value="P-type_ATPase_IIIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01647">
        <property type="entry name" value="ATPase-IIIA_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42861">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 16" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="13 16" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 16" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 16" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 16" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="251"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="285"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="652"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="680"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="717"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="790"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="823"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="17">
        <location>
            <begin position="21"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU362083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ9691784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="949" mass="104315" checksum="E501ADDF3312D968" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZPR3</accession>
    <name>A0AA38ZPR3_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_011916</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9693001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZPR3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000111">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051502">
        <property type="entry name" value="RLP_Defense_Trigger"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48062">
        <property type="entry name" value="RECEPTOR-LIKE PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48062:SF52">
        <property type="entry name" value="RECEPTOR-LIKE PROTEIN 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="672"/>
            <end position="695"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9693001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="732" mass="82909" checksum="931FCA8947D93EA5" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZQG0</accession>
    <name>A0AA38ZQG0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">PVL29_012234</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="23">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9693381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZQG0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000041">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001347">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000416">
        <property type="entry name" value="Probable leucine-rich repeat receptor-like protein kinase At5g63930"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001646">
        <property type="entry name" value="Putative inactive leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000213">
        <property type="entry name" value="Tyrosine-sulfated glycopeptide receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046956">
        <property type="entry name" value="RLP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063">
        <property type="entry name" value="LRR RECEPTOR-LIKE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063:SF16">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="14 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 20" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041372813" description="non-specific serine/threonine protein kinase" evidence="20">
        <location>
            <begin position="23"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="981"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="21">
        <location>
            <begin position="34"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="22">
        <location>
            <begin position="107"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="22">
        <location>
            <begin position="311"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAJ9693381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1037" mass="115896" checksum="049CB5EF2FB76588" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZRN3</accession>
    <name>A0AA38ZRN3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PLAT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_009612</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9693739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZRN3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.60.20">
        <property type="entry name" value="PLAT/LH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001024">
        <property type="entry name" value="PLAT/LH2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036392">
        <property type="entry name" value="PLAT/LH2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31718:SF47">
        <property type="entry name" value="OS06G0206401 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31718">
        <property type="entry name" value="PLAT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01477">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49723">
        <property type="entry name" value="Lipase/lipooxygenase domain (PLAT/LH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50095">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PLAT" evidence="2">
        <location>
            <begin position="29"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9693739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="180" mass="19536" checksum="D3B33EFEDB6DDF24" modified="2024-01-24" version="1">MFPQSMHKRGLFLFKKKVLAIQRLCNDNCVYALYVKTGSAPKAGTDSNISITLSDPGGKSVPVSDLESWGLTGSNHNYYERGNMDVFSGRGPCIGAPICRLNLTSDGSGEHHGWYCDYVEVTATGPHRPCGQTIFYVDQWLASDAPPYQLTAVVDGCRLENIAAKSGRLVLRNSLGSASM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZUY6</accession>
    <name>A0AA38ZUY6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Strictosidine synthase conserved region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_010732</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the strictosidine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZUY6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000032">
        <property type="entry name" value="Protein STRICTOSIDINE SYNTHASE-LIKE 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018119">
        <property type="entry name" value="Strictosidine_synth_cons-reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10426:SF69">
        <property type="entry name" value="PROTEIN STRICTOSIDINE SYNTHASE-LIKE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10426">
        <property type="entry name" value="STRICTOSIDINE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20067">
        <property type="entry name" value="SSL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03088">
        <property type="entry name" value="Str_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63829">
        <property type="entry name" value="Calcium-dependent phosphotriesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041384280" description="Strictosidine synthase conserved region domain-containing protein" evidence="6">
        <location>
            <begin position="21"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="Strictosidine synthase conserved region" evidence="7">
        <location>
            <begin position="159"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9695405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="370" mass="40949" checksum="56FA476658B2C24D" modified="2024-01-24" version="1">MNAKLTLTAITLAAISIILAVNSNHLFKPPSIPGTHDLLHASEVIQVTGAFGPESIAFDPKGEGPYTGVADGRVLKWEGDGRGWTDFAVTTSERKECVRPFAPEMEHICGRPLGLRFDKKTGDLYIADAYFGLQVVEPNGGLATPLVTEVEGRRLLFTNDMDIDEVEDVIYFTDTSTDFHRRQFMASLLSGDNTGRLMKYDKSSKEVTVLLRGLAFANGVAMSKDHSFVLVAETTTGKIIRYWLKGPNAGKSDIFAEVPGYPDNVRRNSKGEFWVALHSKKGPHANWITSNSWVGRTLLKLPLTFKQLHKLIVVEAHATAIKLSEEGQVLEVLEDCEGKSMRFISEVEEHNGKLWLGSVMMPFIGVYNLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZWF5</accession>
    <name>A0AA38ZWF5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">soluble epoxide hydrolase</fullName>
            <ecNumber evidence="2">3.3.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_008657</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Epoxide hydrolase involved in the biosynthesis of cucurbitacin and mogroside tetracyclic triterpene natural products (e.g. siamenoside I and mogrosides IV, V and VI). Cucurbitacins have cytotoxic properties and exhibit deterrent taste as a defense barrier against herbivores. Mogrosides are nonsugar highly oxygenated compounds used as high-intensity zero-calorie sweeteners; they also possess pharmacological properties such as regulating immunity, lowering blood sugar and lipid levels, protecting the liver, and acting as antioxidants and antitumor agents. Catalyzes the hydrolysis of aromatic epoxide-containing substrates, such as the conversion of 24,25-epoxycucurbitadienol to 24,25-dihydroxycucurbitadienol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(24S)-24,25-epoxycucurbitadienol + H2O = (24R)-24,25-dihydroxycucurbitadienol</text>
            <dbReference type="Rhea" id="RHEA:81855"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:229949"/>
            <dbReference type="ChEBI" id="CHEBI:229950"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:81856"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>an epoxide + H2O = an ethanediol</text>
            <dbReference type="Rhea" id="RHEA:19037"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32955"/>
            <dbReference type="ChEBI" id="CHEBI:140594"/>
            <dbReference type="EC" id="3.3.2.10"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:19038"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Secondary metabolite biosynthesis; terpenoid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the AB hydrolase superfamily. Epoxide hydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.3.2.10" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZWF5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004301">
        <property type="term" value="F:epoxide hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000161">
        <property type="entry name" value="Epoxide hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43329">
        <property type="entry name" value="EPOXIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="8">
        <location>
            <begin position="25"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00051067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00058358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00093212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9696543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="317" mass="36281" checksum="AC45D08BEF520B31" modified="2024-01-24" version="1">MEKIQHRNLRVNGINMHVAEIGEGPPILFLHGFPELWYSWRHQLLSLSNLGYRAIAPDLRGYGDSDAPPSPASYTALHIVGDLIALLDSLRLGQVFLVGHDWGAAIAWYFCLLRPDRVKALVNMSVVFRPRNPTRKPIESMRGLFGDDYYMCRFQEPGLAENEFARLGTETVMKIFLASRNPRPPRMPKESWYSGRLKTPIALPPWLSQEDINYFASKFNQNGFTGGLNYYRALDLSWEVLAPWTGVPIKVPVKFIVGDLDTTYNTPGVKEYIHNGGFKKEVPFLQELVIMEGVGHFINQERPEEINAHIYDFIRRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZX22</accession>
    <name>A0AA38ZX22_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_008902</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the peptidase A1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZX22"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05476">
        <property type="entry name" value="pepsin_A_like_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000029">
        <property type="entry name" value="Aspartyl protease family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000016">
        <property type="entry name" value="Probable aspartic protease At2g35615"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034161">
        <property type="entry name" value="Pepsin-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051708">
        <property type="entry name" value="Plant_Aspart_Prot_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032799">
        <property type="entry name" value="TAXi_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032861">
        <property type="entry name" value="TAXi_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47967:SF23">
        <property type="entry name" value="OS04G0448300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47967">
        <property type="entry name" value="OS07G0603500 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14541">
        <property type="entry name" value="TAXi_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14543">
        <property type="entry name" value="TAXi_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 8" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041374025" description="Peptidase A1 domain-containing protein" evidence="9">
        <location>
            <begin position="22"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="10">
        <location>
            <begin position="97"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="115"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9696903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="436" mass="46745" checksum="16EDACFB2CDA0D43" modified="2024-01-24" version="1">MASSAFHMIIVILLALAVSSALFSPASSTSRGLDRRPEKNGFRVSLRHVDSDGNYTKFERIQRAVKRGRLRLQRLSAKTASFEPSVEAPVQAGHGEFLMNLAIGTPAETYSAIMDTGSDLIWTQCKPCKACFDQPTPIFDPEKSSSFSKLPCSSDLCAALQISSCSDGCEYYYSYMGHSSTQGVLATETFTFGDASVSKIGFGCGEENHEPANSQGAGLVGLGRGRLSLISQLGESKFSYCLTSFDDSKGISTLLVGSEATVKSAIPTPLIQNPSHPSFYYLSLEGISVGDTLLLIEKSTFSIQDDGSGGLIIDSGTTITYLKDSAFAALKNEFISQMNLDVDASGSTGLELCFTLPPDASTVDVPQLVFHFEGVDLKLPTENYILADSSLGVICLMMGSSSGMSIFGNFQQQNIVVQHDLEKETISFAPAQCNQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZYD9</accession>
    <name>A0AA38ZYD9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BED-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_008683</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZYD9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46951">
        <property type="entry name" value="BED-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46951:SF2">
        <property type="entry name" value="BED-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="116"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="73"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="127"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9696583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="138" mass="15691" checksum="2D666BD552E65B94" modified="2024-01-24" version="1">MDNESETQVDSSASGRRDPGWRYVRLVNEKDLNTIICICCDKVIKEGICRHKQHLVGGYRNAKKCRKCPEHVKEEMEEYMSSKESQKEQMNMENEDVNEDLFGLEDEDFGEEINSKMNVTNISSGGSNRGGSGGRMFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZYQ3</accession>
    <name>A0AA38ZYQ3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phospholipid/glycerol acyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_008761</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GPAT/DAPAT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZYQ3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090447">
        <property type="term" value="F:glycerol-3-phosphate 2-O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010143">
        <property type="term" value="P:cutin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06551">
        <property type="entry name" value="LPLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056462">
        <property type="entry name" value="HAD_RAM2/GPAT1-8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15486">
        <property type="entry name" value="ANCIENT UBIQUITOUS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15486:SF62">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23270">
        <property type="entry name" value="HAD_RAM2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="188"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="391"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="9">
        <location>
            <begin position="454"/>
            <end position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9696704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="652" mass="74088" checksum="3EAF8DA75ACE0137" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZZ47</accession>
    <name>A0AA38ZZ47_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Selenoprotein O</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_007133</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SELO family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9697862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZZ47"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009534">
        <property type="term" value="C:chloroplast thylakoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070733">
        <property type="term" value="F:AMPylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00692">
        <property type="entry name" value="SelO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003846">
        <property type="entry name" value="SelO"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000658">
        <property type="entry name" value="PRK00029.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32057">
        <property type="entry name" value="PROTEIN ADENYLYLTRANSFERASE SELO, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32057:SF14">
        <property type="entry name" value="PROTEIN ADENYLYLTRANSFERASE SELO, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02696">
        <property type="entry name" value="SelO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9697862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="651" mass="72510" checksum="F8B1248D8FF818FB" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZZX1</accession>
    <name>A0AA38ZZX1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">MBD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_007484</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZZX1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.890.10">
        <property type="entry name" value="Methyl-cpg-binding Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001739">
        <property type="entry name" value="Methyl_CpG_DNA-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01429">
        <property type="entry name" value="MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50982">
        <property type="entry name" value="MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MBD" evidence="7">
        <location>
            <begin position="91"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="156"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="85"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9698415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="207" mass="23424" checksum="B270C7AF7E620586" modified="2024-01-24" version="1">MIGRNEIDYEVFVDCTPLHNYVPNDPVLEAMQNQRKRLKSFEGKSGQVDEASVKQLFHAHQTPRENRDKVPAVASDQLLVAQPETKADHKGRRHDERPSWLPPDWHVEKETRLQGKKAGCLDSFFVDPMGNRFRSKKKVLSFLEAEVLTRRTANKNPNLAMANGGGEGGRWKRPAGSSSRNLTMDEPRFCRLAPDGTLGPHTLDEIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A4H0</accession>
    <name>A0AA39A4H0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Retrotransposon Copia-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_005888</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A4H0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47481">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47481:SF5">
        <property type="entry name" value="RIBONUCLEASE H-LIKE DOMAIN, GAG-PRE-INTEGRASE DOMAIN, GAG-POLYPEPTIDE OF LTR COPIA-TYPE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9700302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="108" mass="12540" checksum="E525218869D0AC07" modified="2024-01-24" version="1">MKLDNGNYLIWKNQLLNVIIVNGLDDFIDGSRPCPPRFLDLQQQILNLEYSVWHRYNRLLMSWLYASLSEDIMTQIVGYSTAVEIWNALNQIYSASSMARVTGLRTKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A4U2</accession>
    <name>A0AA39A4U2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">YTH domain-containing family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_005652</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, and regulates mRNA stability. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in mRNA stability and processing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the YTHDF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9699899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A4U2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990247">
        <property type="term" value="F:N6-methyladenosine-containing RNA reader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061157">
        <property type="term" value="P:mRNA destabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21134">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.590.10:FF:000001">
        <property type="entry name" value="YTH domain family 1, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.590.10">
        <property type="entry name" value="ph1033 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007275">
        <property type="entry name" value="YTH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045168">
        <property type="entry name" value="YTH_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12357:SF92">
        <property type="entry name" value="YTH DOMAIN-CONTAINING FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12357">
        <property type="entry name" value="YTH YT521-B HOMOLOGY DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04146">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50882">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="YTH" evidence="6">
        <location>
            <begin position="375"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="166"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="225"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="310"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="580"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="225"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="313"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="580"/>
            <end position="589"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU369095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9699899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="714" mass="79101" checksum="C3CA34F4343872B1" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A770</accession>
    <name>A0AA39A770_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_006483</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A770"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36402">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36402:SF1">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9701158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="197" mass="23127" checksum="305215D730DC1ABE" modified="2024-01-24" version="1">MAVAASRNTLHRYSAEIFLKPTLTRTLSLRFNSTSTHSTPQDHTYTDPNSFIGSWKAPPDPKVAQAKLAGLRRDYAKKVKELRLQYIHEVELQREDKRRKDEAKREAARRAQAEIKASKAAAAQARAAERKVFEEEFRQTLLKERTEKLENWRMKEKLMDEKRKEKNELLRRQSSIWVDERDLEKKILEAIVDTTHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A9G3</accession>
    <name>A0AA39A9G3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Expansin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_004777</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Causes loosening and extension of plant cell walls by disrupting non-covalent bonding between cellulose microfibrils and matrix glucans. No enzymatic activity has been found.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the expansin family. Expansin A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A9G3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009664">
        <property type="term" value="P:plant-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002963">
        <property type="entry name" value="Expansin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007117">
        <property type="entry name" value="Expansin_CBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036749">
        <property type="entry name" value="Expansin_CBD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31867">
        <property type="entry name" value="EXPANSIN-A15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01357">
        <property type="entry name" value="Expansin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01226">
        <property type="entry name" value="EXPANSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49590">
        <property type="entry name" value="PHL pollen allergen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0134">Cell wall</keyword>
    <keyword evidence="1" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Expansin-like CBD" evidence="2">
        <location>
            <begin position="30"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU365023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9703144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="66" mass="7214" checksum="501D10226EAF9C9B" modified="2024-01-24" version="1">MPMFFKIIEYRARIIPVAFILVPCCEQGGIGFTINSFRYFNLVLITNVAGAGDIMRASVKGSKIGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ACD6</accession>
    <name>A0AA39ACD6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Malectin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_003193</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ACD6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024788">
        <property type="entry name" value="Malectin-like_Carb-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45631:SF45">
        <property type="entry name" value="LEUCINE-RICH REPEAT (LRR) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45631">
        <property type="entry name" value="OS07G0107800 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12819">
        <property type="entry name" value="Malectin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041458425" description="Malectin-like domain-containing protein" evidence="7">
        <location>
            <begin position="23"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="Malectin-like" evidence="8">
        <location>
            <begin position="23"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="217"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="228"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9705030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="421" mass="46562" checksum="241E7071BF6BC3D6" modified="2024-01-24" version="1">MPFYHLSFFFLCLVTIPPCCVLLNCGPTNEIVTTNLKFITDEGFISVGNTSTLNTPDLLSILSTLRYFPDKSAKKYGYMIPVIKGGKYLIRTAYYYGGFGRNEPLVFEQNIDGTKWGIVNMTEDYAKGLTSYYEIVAAAMRKMLSICLARNGKTVSNPFITALELENMEAFVYSSTDFTQYALNIVARHNFGTNNNNISPSNITPSDFWNMPPLKAFEGSNHKKQRKGTSSPAPTTSQTKKTTSARESLPSPFSWRVFSVSVNGKNFFTNLNVTTDGEMIVMTPGADIPIGPVINAGEVFQMLPLGQRTLTRDDSHSLHFFYSYTLIRIIWRQHSSDWSGDPCLPQNNSWTGVTCTPGKLARVVTLNLTNFILAGLLSPSIANLTALLAGGDKPLGPTPEMSTLNELRKMLSPQKLAKLNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ACK9</accession>
    <name>A0AA39ACK9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">histidinol-phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Histidinol-phosphate phosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_002996</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-histidinol phosphate + H2O = L-histidinol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14465"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57699"/>
            <dbReference type="ChEBI" id="CHEBI:57980"/>
            <dbReference type="EC" id="3.1.3.15"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 8/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the inositol monophosphatase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.15" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ACK9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004401">
        <property type="term" value="F:histidinol-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01641">
        <property type="entry name" value="Bacterial_IMPase_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.540.10:FF:000021">
        <property type="entry name" value="Inositol monophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.80:FF:000013">
        <property type="entry name" value="Inositol monophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.10">
        <property type="entry name" value="Fructose-1,6-Bisphosphatase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011809">
        <property type="entry name" value="His_9_proposed"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020583">
        <property type="entry name" value="Inositol_monoP_metal-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051090">
        <property type="entry name" value="Inositol_monoP_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02067">
        <property type="entry name" value="his_9_HisN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43200:SF6">
        <property type="entry name" value="3'(2'),5'-BISPHOSPHATE NUCLEOTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43200">
        <property type="entry name" value="PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00377">
        <property type="entry name" value="IMPHPHTASES"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56655">
        <property type="entry name" value="Carbohydrate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00629">
        <property type="entry name" value="IMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="12">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR600760-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9704734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="323" mass="35475" checksum="6C52BB01A26D921B" modified="2024-01-24" version="1">MLSQTSAFVSYSSEFRSVRFSANLSTSALGRTSLSLPMASNSELSNQIQNISLGLNDSELDRFADVGNKVADAAGEVIRGYFRKKFDILQKEDLSPVTIADQAAEESMVSILLESFPSHAIYGEENGWRCKEKFADYVWVLDPIDGTKSFITGKPLFGTLIALLHKGRPILGIIDQPVLHERWIGISGRKTTLNGQEVATRDCAKLAQAYLYTTSPHLFSGDAEDAFARVRNKVKVPLYGCDCYAYALLASGYVDLVIESGLKPYDFLSLVPVIEGAGGVITDWKGQQLYWEASSDSHAPSFNVVAAGDEELHQQALDSLQWQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ACU1</accession>
    <name>A0AA39ACU1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GRAM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_003357</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GEM family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ACU1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037848">
        <property type="entry name" value="GEM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004182">
        <property type="entry name" value="GRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31969">
        <property type="entry name" value="GEM-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02893">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00568">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GRAM" evidence="2">
        <location>
            <begin position="88"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9705258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="214" mass="24361" checksum="D94A79CE16F1A4E9" modified="2024-01-24" version="1">MKNQLQEHIIGIPVSCQYDKPSPPNGSGAIIPKKMNSMNYRMNNNRKKADNFVDGVREHVRLGLKFSETVKGKLSLGARILQIGGVKRVFRQNFGVREGEKLLKVSQCYLSTTAGPIAGLLFISTQRVAFCSERSIKFSSPNGELVRIHYKVSIPLRKIKRANQSENVQKPSQKYMEIVTVDNFDFWFMGFLNYQKAFSYLRQAISQVQNPVQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AD48</accession>
    <name>A0AA39AD48_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Serine carboxypeptidase-like 18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_003391</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AD48"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019748">
        <property type="term" value="P:secondary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12670:FF:000002">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000072">
        <property type="entry name" value="Serine carboxypeptidase-like 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF377">
        <property type="entry name" value="SERINE CARBOXYPEPTIDASE-LIKE 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041332752" description="Serine carboxypeptidase-like 18" evidence="2">
        <location>
            <begin position="34"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9705332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="469" mass="53074" checksum="C731C5896370B758" modified="2024-01-24" version="1">MAAKRPSNSSMFHGGLFLRLLLVVLVSANAASAGSIVEYLPGYGKLDFKLETGYVSVGDSELFYYFIESQGNPTTDPFFLWLTGGPGCSSFNGLIYEIGPMEFDIHNYPGGLPRLLPYKYAWTRTASILFLDAPVGTGFSYSTSADGWSSSDTDSALETYKFLRKWLIDHPKYLPLQLYVGGDSYSGIIVPLVVKHIVDAIDDHTVPRFNLQGYLVGSPTTDETINTNAKVVFAHRLALISDELYEAAKESCNGNYADVDPSNTKCLSSLGAIDHCVKDLFRNDILEPKCVFESPEPSRRSLDEKSGDFILNTPRLEEYWCRNFNYALAYIWANDESVQEALNVRVGTVKYWSRCNKSLSYTKDVESVIDVHRFLSKKQLEVLVEVGDRDLVVPYPGAVEWIRLLNLTIVNPWRPWFVDGEIAGYTEKHSENGYRLTYAIVKGAGHTAPEYYRRECYYMFKRWLHYYPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ADD2</accession>
    <name>A0AA39ADD2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Plant basic secretory protein (BSP) family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_003285</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ADD2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007541">
        <property type="entry name" value="Uncharacterised_BSP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33321">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33321:SF12">
        <property type="entry name" value="PLANT BASIC SECRETORY PROTEIN (BSP) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04450">
        <property type="entry name" value="BSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041462393" description="Plant basic secretory protein (BSP) family protein" evidence="1">
        <location>
            <begin position="24"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9705154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="227" mass="25623" checksum="D89126D88747144D" modified="2024-01-24" version="1">MAPKHMFLLSCLVILAALHGIHAVEFEVINNAESSAGGIRFTNEIGAEFSMSTLSISTHFIWNLFQQNTEPDRKDVQKVTLIIDNMDGIAYTANDEIHFNANYIGNYSGDLRMEFSGIIFHEMTHIWQWYGDGQAPGGLIEGIADFVRLSAGYAPSHWVQPGEGERWDQGYDVTARFLDYCNGLSSGFVAELNKKMESDYSEDFFVEQLGETVDELWSDYKDMYGIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ADD8</accession>
    <name>A0AA39ADD8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Triosephosphate isomerase, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_002775</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="4">Carbohydrate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the triosephosphate isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ADD8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004807">
        <property type="term" value="F:triose-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046166">
        <property type="term" value="P:glyceraldehyde-3-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019563">
        <property type="term" value="P:glycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00311">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000025">
        <property type="entry name" value="Triosephosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00147_B">
        <property type="entry name" value="TIM_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035990">
        <property type="entry name" value="TIM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022896">
        <property type="entry name" value="TrioseP_Isoase_bac/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000652">
        <property type="entry name" value="Triosephosphate_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020861">
        <property type="entry name" value="Triosephosphate_isomerase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00419">
        <property type="entry name" value="tim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21139">
        <property type="entry name" value="TRIOSEPHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21139:SF2">
        <property type="entry name" value="TRIOSEPHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00121">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51351">
        <property type="entry name" value="Triosephosphate isomerase (TIM)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00171">
        <property type="entry name" value="TIM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51440">
        <property type="entry name" value="TIM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9704368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="313" mass="33862" checksum="4BEAF19A25042803" modified="2024-01-24" version="1">MAAVTSTSLASQLSTPKATTPSYSYSHATQFSGLRRSFSNLDHSPQPFFSSNLRISSSPRKPCRGIVAMAGSGKFFVGGNWKCNGTKDSISKLVSDLNSAKLEADVDVVVAPPFVYLDQVKNSLTDRIEISAQNSWIGKGGAFTGEISVEQLKDTGCNWVILGHSERRHVIGEDDQFIGKKAAYALSQGLKVIACIGELLQEREAGKTFDVCFQQMKAFADAVPSWDDVVVAYEPVWAIGTGKVATPQQAQEVHIAVRDWLKKNVSAEVASKTRIIYGGSVNGSNCSELAKQEDIDGFLVGGASLKVYFYCWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AE11</accession>
    <name>A0AA39AE11_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein FAR1-RELATED SEQUENCE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_003828</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Putative transcription activator involved in regulating light control of development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the FHY3/FAR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AE11"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004330">
        <property type="entry name" value="FAR1_DNA_bnd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031052">
        <property type="entry name" value="FHY3/FAR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018289">
        <property type="entry name" value="MULE_transposase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006564">
        <property type="entry name" value="Znf_PMZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31669">
        <property type="entry name" value="PROTEIN FAR1-RELATED SEQUENCE 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31669:SF280">
        <property type="entry name" value="PROTEIN FAR1-RELATED SEQUENCE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03101">
        <property type="entry name" value="FAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10551">
        <property type="entry name" value="MULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04434">
        <property type="entry name" value="SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00575">
        <property type="entry name" value="ZnF_PMZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="8">
        <location>
            <begin position="544"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9705903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="835" mass="95550" checksum="C6A3195CD0F1A505" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AH26</accession>
    <name>A0AA39AH26_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_002514</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AH26"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47950">
        <property type="entry name" value="CYTOCHROME P450, FAMILY 76, SUBFAMILY C, POLYPEPTIDE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47950:SF44">
        <property type="entry name" value="CYTOCHROME P450, FAMILY 76, SUBFAMILY C, POLYPEPTIDE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0349">Heme</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="2">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9707511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="152" mass="17569" checksum="2A0ACC04191D4C8B" modified="2024-01-24" version="1">MAKLLRNPHVMQNRIELSEVISLGYSVKKSDIDRLPYFQAVVKETMRFHPLVPLLLPYKAKNDLEICGFTIPKDNHVLVNIWAIARDLGYWKDPLSFLLERFLGCNIDFRGRDFEYLPFGAGKRICLSIPLGLRMVHLVLASIIRSFSRKLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AIT7</accession>
    <name>A0AA39AIT7_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_000383</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AIT7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046467">
        <property type="entry name" value="PHL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021950">
        <property type="entry name" value="Spt20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046468">
        <property type="entry name" value="Spt20-like_SEP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13526:SF23">
        <property type="entry name" value="PROTEIN PHYTOCHROME-DEPENDENT LATE-FLOWERING-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13526">
        <property type="entry name" value="TRANSCRIPTION FACTOR SPT20 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20474">
        <property type="entry name" value="PHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12090">
        <property type="entry name" value="Spt20_SEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Spt20-like SEP" evidence="2">
        <location>
            <begin position="101"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="PHL" evidence="3">
        <location>
            <begin position="573"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="265"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="711"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1150"/>
            <end position="1185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="298"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="720"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1175"/>
            <end position="1185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9708301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1185" mass="129977" checksum="15BC5B4C096B88E1" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AKC0</accession>
    <name>A0AA39AKC0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Homeobox-leucine zipper protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">HD-ZIP protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Homeodomain transcription factor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_000954</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Transcription factor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 10">Belongs to the HD-ZIP homeobox family. Class I subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AKC0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000200">
        <property type="entry name" value="Homeobox-leucine zipper protein ATHB-13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045224">
        <property type="entry name" value="HDZip_class_I_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000047">
        <property type="entry name" value="HTH_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003106">
        <property type="entry name" value="Leu_zip_homeo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24326">
        <property type="entry name" value="HOMEOBOX-LEUCINE ZIPPER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24326:SF176">
        <property type="entry name" value="HOMEOBOX-LEUCINE ZIPPER PROTEIN ATHB-13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02183">
        <property type="entry name" value="HALZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00031">
        <property type="entry name" value="HTHREPRESSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 8 9" id="KW-0371">Homeobox</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="13">
        <location>
            <begin position="79"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="8">
        <location>
            <begin position="81"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="131"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU369038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9709245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="289" mass="33071" checksum="15C89B35C2B8888B" modified="2024-01-24" version="1">MRTSRFPWFYSISLFPSFFLNCSYVYILRLICCHGFSIQIDVSIGAGVASLLGKRSMSFSGIDVCEETNGEDDLSDDGSQAGEKKRRLNMEQVKTLEKNFELGNKLEPERKMQLARALGLQPRQIAIWFQNRRARWKTKQLEKDYDLLKRQFEAVKAENDTLQAQNQKLHAEVLALKSREPTESINLNIKETEGSCSNRSENSSDIKLDISRTPAIDSPLSTHPTSRPLFPSSIRPAGVVQLFQNSSSRPDLQCQKMDQPVKEESFCNMFCGMDDQSGFWPWLEQQHFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D3T5</accession>
    <name>A0AA39D3T5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Wall-associated receptor kinase galacturonan-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_026585</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D3T5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025287">
        <property type="entry name" value="WAK_GUB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33355">
        <property type="entry name" value="WALL-ASSOCIATED RECEPTOR KINASE CARBOXY-TERMINAL PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33355:SF1">
        <property type="entry name" value="WALL-ASSOCIATED RECEPTOR KINASE-LIKE 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13947">
        <property type="entry name" value="GUB_WAK_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041277364" description="Wall-associated receptor kinase galacturonan-binding domain-containing protein" evidence="3">
        <location>
            <begin position="25"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="Wall-associated receptor kinase galacturonan-binding" evidence="4">
        <location>
            <begin position="29"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9670121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="307" mass="32748" checksum="E1D51B9F70C95DF7" modified="2024-01-24" version="1">MEQLLCFIFFHIFIIVFAPQLTFAPEPACRDTCGSLSVKFPFGTGVGCGSPRFQPYISCSQSSDGGDQLLLRTHTGVYPITSISYEAAAFTVTPPLMSNCTCMQPSPNLGLDWASPFQLGPSAFILLSCPPSLASKASPPICDPSLSHLCASIYTCPSVVSLGLPLFPPTNTCCVYSPANFNTKGELDLHSLKCAAYASVVMLGDYPTDPTRWDYGVAFKYTQGKFDNYGMDTKCYACDSSGGVCGYAPPSNSFVCVCGNGFNTSSNCNGYSLAGIWSSGSRPTWKAWWGVLAWLGVLASLTIGIPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D417</accession>
    <name>A0AA39D417_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_027254</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9671183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D417"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009738">
        <property type="term" value="P:abscisic acid-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009651">
        <property type="term" value="P:response to salt stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009414">
        <property type="term" value="P:response to water deprivation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14662">
        <property type="entry name" value="STKc_SnRK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000085">
        <property type="entry name" value="Serine/threonine-protein kinase SRK2E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000045">
        <property type="entry name" value="Serine/threonine-protein kinase SRK2E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343">
        <property type="entry name" value="SERINE/THREONINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343:SF376">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE SRK2A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="4"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="313"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="324"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="339"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9671183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="355" mass="40737" checksum="6CF0DD012DEB0684" modified="2024-01-24" version="1">MEKYEMVKDLGSGNFGVARLMRVKETKELVAMKFIERGHKIDENVAREIINHRSLRHPNIIRFKEVVLTPTHLGIVMEYAAGGELFERICNAGRFSEDEARYFFQQLISGVSYCHSMQICHRDLKLENTLLDGSPAPRLKICDFGYSKSSLLHSRPKSTVGTPAYIAPEVLSRREYDGKLADVWSCGVTLYVMLVGAYPFEDPDDPKNFRKTIGRIMSVQYKIPEYVHISQDCKQLLSRIFVANSSRRITIKEIKNHPWFLKNLPRELTESTQANYYQRDNPSFSLQSVEEIMKIVGEARIRCPSSRSIGGFGWGAEESKGGEDNLDVEEEEIEEEDEYEKRVREVHASGEFHIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D5Y0</accession>
    <name>A0AA39D5Y0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Receptor-like serine/threonine-protein kinase</fullName>
            <ecNumber evidence="13">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PVL29_025528</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="13"/>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D5Y0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048544">
        <property type="term" value="P:recognition of pollen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00028">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01098">
        <property type="entry name" value="PAN_AP_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000060">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.90.10.10:FF:000004">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000195">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.4.10:FF:000002">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.10">
        <property type="entry name" value="Bulb-type lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.4.10">
        <property type="entry name" value="Hepatocyte Growth Factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000858">
        <property type="entry name" value="S_locus_glycoprot_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024171">
        <property type="entry name" value="SRK-like_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32444:SF183">
        <property type="entry name" value="APPLE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32444">
        <property type="entry name" value="BULB-TYPE LECTIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08276">
        <property type="entry name" value="PAN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00954">
        <property type="entry name" value="S_locus_glycop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000641">
        <property type="entry name" value="SRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00473">
        <property type="entry name" value="PAN_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4 15" id="KW-0732">Signal</keyword>
    <keyword evidence="3 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041225030" description="Receptor-like serine/threonine-protein kinase" evidence="15">
        <location>
            <begin position="24"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="441"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="17">
        <location>
            <begin position="24"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="18">
        <location>
            <begin position="339"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="513"/>
            <end position="798"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR000641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ9671919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="823" mass="91981" checksum="38DBDB79B8F4718B" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D8D1</accession>
    <name>A0AA39D8D1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA topoisomerase I catalytic core eukaryotic-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_026308</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D8D1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003917">
        <property type="term" value="F:DNA topoisomerase type I (single strand cut, ATP-independent) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014727">
        <property type="entry name" value="TopoI_cat_a/b-sub_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013500">
        <property type="entry name" value="TopoI_cat_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051062">
        <property type="entry name" value="Topoisomerase_IB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10290:SF23">
        <property type="entry name" value="DNA TOPOISOMERASE 1 BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10290">
        <property type="entry name" value="DNA TOPOISOMERASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01028">
        <property type="entry name" value="Topoisom_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA topoisomerase I catalytic core eukaryotic-type" evidence="2">
        <location>
            <begin position="9"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9672982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="99" mass="11323" checksum="F159C797D71025D9" modified="2024-01-24" version="1">MEEKKEEENKLNKETKEGDVAQKIVVYQHTNKTIKVAIICNHQRTVSKSYAAQMTRLTEKIHELKGVLNRLKTDLARAKKGKPPLKDADGKRRNLTPEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DB16</accession>
    <name>A0AA39DB16_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_022072</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[DNA-directed RNA polymerase] + ATP = phospho-[DNA-directed RNA polymerase] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10216"/>
            <dbReference type="Rhea" id="RHEA-COMP:11321"/>
            <dbReference type="Rhea" id="RHEA-COMP:11322"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43176"/>
            <dbReference type="ChEBI" id="CHEBI:68546"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.23"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9676885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DB16"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070985">
        <property type="term" value="C:transcription factor TFIIK complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008353">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07841">
        <property type="entry name" value="STKc_CDK7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000289">
        <property type="entry name" value="Cyclin-dependent kinase D-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000097">
        <property type="entry name" value="Putative cyclin-dependent kinase 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037770">
        <property type="entry name" value="CDK7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF0">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 14" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="16"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="298"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="386"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="319"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="348"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="138"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="22"/>
            <end position="30"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR637770-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR637770-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9676885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="422" mass="48094" checksum="2B263790AEF3D2C9" modified="2024-01-24" version="1">MSEVPIVLSKKVADRYLKREVLGEGTYGVVSKAIDTKTGQIVAIKKIRLGKHKEGVNFTALREIKLLKELKDPHIIELIDAFPHKGNLHLVFEFMETDLEAVIRDRNIVLSLADIKSFLQMTLKGLAFCHKKWVLHRDMKPNNLLIGDKGQLKLADFGLARIFGSPDRKFTHQVFARWYRAPELLFGSKQYGPGVDVWAAACIFAELLLRRPFLQGSSDIDQLGKIFAAFGTPKPAQWPDMVYLPDYVEYQFVPAPPLRSLFPMASDDALDLLSKMFTYDPKARISVQQALEHRYFTSGPLPTEPAKLPRPHPKRESLNTRVSDFNQQEGPTVLSPPRKSRRVMPQRDGFEENAHQVEKSDERVNDIRQATGERMGWNEHAPMSIDFSVFGSRPPPRPTINSADKSRLKRKLDLEFQQPDQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DBI0</accession>
    <name>A0AA39DBI0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Retrovirus-related Pol polyprotein from transposon 17.6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_024955</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9676207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DBI0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.10.10:FF:000007">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 17.6-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.370:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 17.6-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000020">
        <property type="entry name" value="Transposon Tf2-6 polyprotein-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041373">
        <property type="entry name" value="RT_RNaseH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17917">
        <property type="entry name" value="RT_RNaseH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="8">
        <location>
            <begin position="17"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase catalytic" evidence="9">
        <location>
            <begin position="564"/>
            <end position="678"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9676207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="678" mass="78081" checksum="05BB7D0F4A78FA7E" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DES7</accession>
    <name>A0AA39DES7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_018255</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DES7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36617">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36617:SF15">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase zinc-binding" evidence="1">
        <location>
            <begin position="192"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9682283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="299" mass="34998" checksum="46227CD2F4053707" modified="2024-01-24" version="1">MQRPHLVRWNLVCMEKRKGGLGVRNLALMNRALISKWNWHFANEREAFWKKVINQKYGVEEGDWCTRAVSERYGVGLWNAIRNKWLFFKNRLTYQVGNGRSVKFWKDKWYGDELLCESFPSLYSISLSKDAWVSDVWNPNRDGESWTPLFSRAVNDWEIAMVERFMLKIQAFRVQKEDDDRVVWTASKSGDFSVKSLYSILEPGEAVLFPCNGIWRVTAPPKVAFFAWEASWGKILTLEQVQRRGYFMANRCFLCLSKMETADHLLLHCVKTRVLWNLVFSLFGVAWVLPCTVKDTLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DG81</accession>
    <name>A0AA39DG81_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_020103</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9681037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DG81"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9681037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="145" mass="16409" checksum="B505887EE10DEDAC" modified="2024-01-24" version="1">MVHGFLVGCSFLSIYWDREGCLIRTSAKQSRQIGIFEFCGRNIMGNFFGMMQQVVHPWSHEEQGWKKIPTASCSGVRIKVRMTTTELKELMATVDSSKGSSEMGRLIMRELSKGKYQARVVACGDLMMPVQLSSIREEEDDDKCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DGD7</accession>
    <name>A0AA39DGD7_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_018834</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GLUTAMINE DUMPER 1 (TC 9.B.60) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DGD7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080143">
        <property type="term" value="P:regulation of amino acid export"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040359">
        <property type="entry name" value="GDU"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33228">
        <property type="entry name" value="PROTEIN GLUTAMINE DUMPER 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33228:SF76">
        <property type="entry name" value="PROTEIN GLUTAMINE DUMPER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="47"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="55"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9683004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="100" mass="10965" checksum="4277EC267E6C738C" modified="2024-01-24" version="1">MRPTNTTANSAGFHRWNSPIPYLFCGLGLTLGLIALALVLLACSFGRSPSNSSTQEEKEPVKEVEKQTDMEPRFVVIMPGDDKPKFLAKPAASDRQSEQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DIA8</accession>
    <name>A0AA39DIA8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NAD-dependent epimerase/dehydratase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_017318</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DIA8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08958">
        <property type="entry name" value="FR_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000085">
        <property type="entry name" value="Dihydroflavonol reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF575">
        <property type="entry name" value="NAD-DEPENDENT EPIMERASE_DEHYDRATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="4">
        <location>
            <begin position="10"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9685241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="302" mass="33446" checksum="AC9A724FE4649B75" modified="2024-01-24" version="1">MSVAVAEKVVCVTGAAGFIASWLVKLLLLRGYTVKATLRNPDDPTKTEHLLALEGAKERLHLFKADLLEEGSFDSIVEGCEGIFHTASPVLLEVANPKVELIDPAVNGTLNVLRSCTKVPSIRRVVVTSSIVAAIFNGKTLTPDVMVDETWFSDPAFCEESKLWYVLSKTLAEEAAWKFAKENGIDLVTMNPGFVIGPFLQPTINLTMEIILNLINAFEISSASGRYCLVERVTYCSEAIKILQELYPALHLPQKNAEDEPPMPTYQISKEKVKSLAIDFIPLEMSLKDTMENLKEKNFIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DJR7</accession>
    <name>A0AA39DJR7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_016887</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DJR7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057670">
        <property type="entry name" value="SH3_retrovirus"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439">
        <property type="entry name" value="GAG-POL-RELATED RETROTRANSPOSON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439:SF467">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25597">
        <property type="entry name" value="SH3_retrovirus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9684637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="737" mass="83685" checksum="B07DF7662D3DB05D" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DKA1</accession>
    <name>A0AA39DKA1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mediator complex subunit 15 KIX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_017285</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DKA1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.246.20:FF:000003">
        <property type="entry name" value="Mediator of RNA polymerase II transcription subunit 15a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.20">
        <property type="entry name" value="Coactivator CBP, KIX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036529">
        <property type="entry name" value="KIX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036546">
        <property type="entry name" value="MED15_KIX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044661">
        <property type="entry name" value="MED15a/b/c-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33137">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 15A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33137:SF4">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 15A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16987">
        <property type="entry name" value="KIX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47040">
        <property type="entry name" value="Kix domain of CBP (creb binding protein)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mediator complex subunit 15 KIX" evidence="4">
        <location>
            <begin position="41"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="11"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="116"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="18"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="116"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="129"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9685192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="152" mass="17076" checksum="70E0DD090F823459" modified="2024-01-24" version="1">MTFRIRKARVSLKLASHPTHSQSQDKNNQRPAQAEPVMDGGDWRTHLSPDSRHRIVNKIMDTLKRHLPVYGPEGLHELRKIAERFEEKIYSAATSQSDYLRKIALKMLTMDTKSFNAATNSLPSNSAGHSKKSPNGKKSSARKKSPRSSNGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DKL0</accession>
    <name>A0AA39DKL0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Trichome birefringence-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_017510</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PC-esterase family. TBL subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DKL0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016413">
        <property type="term" value="F:O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029962">
        <property type="entry name" value="TBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026057">
        <property type="entry name" value="TBL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025846">
        <property type="entry name" value="TBL_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285:SF36">
        <property type="entry name" value="PROTEIN TRICHOME BIREFRINGENCE-LIKE 38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285">
        <property type="entry name" value="PROTEIN TRICHOME BIREFRINGENCE-LIKE 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13839">
        <property type="entry name" value="PC-Esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14416">
        <property type="entry name" value="PMR5N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041413260" description="Trichome birefringence-like N-terminal domain-containing protein" evidence="7">
        <location>
            <begin position="27"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="Trichome birefringence-like N-terminal" evidence="9">
        <location>
            <begin position="37"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="Trichome birefringence-like C-terminal" evidence="8">
        <location>
            <begin position="92"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF14416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9685492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="356" mass="40971" checksum="99BCF8C995C551B9" modified="2024-01-24" version="1">MGFWVQGWSCILVGVAMIFSGGMCLAKGEVGEGEEGKCDIYEGSWVVDESYPMYNSTLCPHIRREFDCQKYGRLDNLYLRYRWQPKTVICPDFLRRFKGKKIMYIGDSISVNQWQSMVCLLHAVVSDQSRIFQNVNGPITTVIFQDYDLSIMVFRSPYLVDIEIEEIGRVLKLDSLKNGDIWKTNDIVIFNTWLWWYRSGRAQPWDYIQDGDQIKKDMDRMVAFKKALITWAKWVDSNVDFNKTQVFFRGVSPTHYDGKEWDEPRVTNCSKETQPISGSTYPSGLPQSSYVVEGVLSGVPKPVQFLNITTLSQLRKDGHPSMYNGVNRSMDCTHWCIAGVPDTWNQLLYAALISQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DLT9</accession>
    <name>A0AA39DLT9_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_014745</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ARG7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9689231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DLT9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009733">
        <property type="term" value="P:response to auxin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003676">
        <property type="entry name" value="SAUR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31374">
        <property type="entry name" value="AUXIN-INDUCED PROTEIN-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31374:SF203">
        <property type="entry name" value="AUXIN-RESPONSIVE PROTEIN SAUR71-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02519">
        <property type="entry name" value="Auxin_inducible"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9689231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="163" mass="18483" checksum="E58A181CDB59E2E2" modified="2024-01-24" version="1">MASAVKRVEKIRQIVRLKQVMQRWKTMSVSLRPRSIRSFSDSDSDCNSGSIRRTPSGFLAVYVGVDRRRFVIPTRLLNLPIFVALLNKAEEEFGLRSSGGLVFPCEVGFFKEVLRFLEKDEAKYGGLGLDEFVKMVSEVGFDSCRETRNVVNGFTPLLQKARV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DME6</accession>
    <name>A0AA39DME6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cystatin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_012540</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9689921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DME6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004869">
        <property type="term" value="F:cysteine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00042">
        <property type="entry name" value="CY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000010">
        <property type="entry name" value="Cystatin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046350">
        <property type="entry name" value="Cystatin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018073">
        <property type="entry name" value="Prot_inh_cystat_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47364">
        <property type="entry name" value="CYSTEINE PROTEINASE INHIBITOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47364:SF2">
        <property type="entry name" value="CYSTEINE PROTEINASE INHIBITOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16845">
        <property type="entry name" value="SQAPI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00043">
        <property type="entry name" value="CY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54403">
        <property type="entry name" value="Cystatin/monellin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00287">
        <property type="entry name" value="CYSTATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0789">Thiol protease inhibitor</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041317722" description="Cystatin domain-containing protein" evidence="3">
        <location>
            <begin position="25"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Cystatin" evidence="4">
        <location>
            <begin position="29"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9689921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="118" mass="12968" checksum="660763D93DBBCC53" modified="2024-01-24" version="1">MAWGQNRLFLFLTAALLLLNGGSAARTEALVGGWRPIKNISDPHVQEIGEFAVTEHNKQATASLKFQSVVSGETQVVSGTNYRLVVVAEDGGVSNKYEAVVWEKPWMGFRNLTSFTRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DMU5</accession>
    <name>A0AA39DMU5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">MLO-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">MLO</name>
        <name evidence="10" type="ORF">PVL29_016308</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">May be involved in modulation of pathogen defense and leaf cell death.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="8">The C-terminus contains a calmodulin-binding domain, which binds calmodulin in a calcium-dependent fashion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the MLO family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DMU5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004326">
        <property type="entry name" value="Mlo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31942">
        <property type="entry name" value="MLO-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31942:SF54">
        <property type="entry name" value="MLO-LIKE PROTEIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03094">
        <property type="entry name" value="Mlo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="6 8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 8" id="KW-0568">Pathogenesis-related protein</keyword>
    <keyword evidence="4 8" id="KW-0611">Plant defense</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="61"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="147"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="267"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="292"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="347"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="390"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU280816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9687787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="438" mass="50531" checksum="3967F64E60CEF01F" modified="2024-01-24" version="1">MAEDESRSLEFTSTWIVAVVCSIIVLVSFAAERGLHRLGKFLLHKSQDALFEALQKLKEELMLLGFISLLLTVVQGPITKICIPDDVASHMLPCKMPTSHVSESYHRRYPPQTVNSIGKRLLLESSTGQHCAQGKVPLLSLEALHHLHIFIFVLAVVHVIFCATTMVLGGAKIRQWKKWEDSIRNEPVSRRNESVPELAERKETVHWRKSAIVSWMMSFFKQFYGSVTKSDYITLREGFIAAHCASNPKFDFHNYMMRTLEVDFKKVVGISWYLWLFVVFFLLLDVEGWHTYFWLAFLPLILLLLVGAKLEHIITRLAQEVDDEKAKGLEASATPSDEHFWFRRPSIVLYLIHFILFQNSFEIAFFFWIWSTYGFNSCIMEKLGYIIPRLLMGVLVQVLCSYSTLPLYVIVTQMGSMFKEGMFQQHVHQSLVVWARGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DMV1</accession>
    <name>A0AA39DMV1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">L-dopachrome isomerase</fullName>
            <ecNumber evidence="9">5.3.2.1</ecNumber>
            <ecNumber evidence="8">5.3.3.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">L-dopachrome tautomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Phenylpyruvate tautomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_014814</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>3-phenylpyruvate = enol-phenylpyruvate</text>
            <dbReference type="Rhea" id="RHEA:17097"/>
            <dbReference type="ChEBI" id="CHEBI:16815"/>
            <dbReference type="ChEBI" id="CHEBI:18005"/>
            <dbReference type="EC" id="5.3.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-dopachrome = 5,6-dihydroxyindole-2-carboxylate</text>
            <dbReference type="Rhea" id="RHEA:13041"/>
            <dbReference type="ChEBI" id="CHEBI:16875"/>
            <dbReference type="ChEBI" id="CHEBI:57509"/>
            <dbReference type="EC" id="5.3.3.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MIF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.2.1" evidence="9"/>
    <dbReference type="EC" id="5.3.3.12" evidence="8"/>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9689320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DMV1"/>
    <dbReference type="SMR" id="A0AA39DMV1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004167">
        <property type="term" value="F:dopachrome isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050178">
        <property type="term" value="F:phenylpyruvate tautomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.429.10:FF:000004">
        <property type="entry name" value="Tautomerase/MIF superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.429.10">
        <property type="entry name" value="Macrophage Migration Inhibitory Factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001398">
        <property type="entry name" value="Macrophage_inhib_fac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014347">
        <property type="entry name" value="Tautomerase/MIF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11954">
        <property type="entry name" value="D-DOPACHROME DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11954:SF6">
        <property type="entry name" value="MACROPHAGE MIGRATION INHIBITORY FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01187">
        <property type="entry name" value="MIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55331">
        <property type="entry name" value="Tautomerase/MIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0202">Cytokine</keyword>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00036735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00036823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00042730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9689320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="115" mass="12284" checksum="BF1089BA9018E5FB" modified="2024-01-24" version="1">MPTLNLFTNVPVDAVVASDILKDCTKAVAKIIGKPESYVMILLNGGVPIEFAGTEEPAAYGELISIGGLGPSVNGKLSSTIAEIIQTKLSIDSARFYVKFYDVERSFFGFNGSTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DPW9</accession>
    <name>A0AA39DPW9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_011736</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DPW9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000438">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054722">
        <property type="entry name" value="PolX-like_BBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039537">
        <property type="entry name" value="Retrotran_Ty1/copia-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057670">
        <property type="entry name" value="SH3_retrovirus"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648">
        <property type="entry name" value="TRANSPOSASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648:SF28">
        <property type="entry name" value="TRANSPOSON-ENCODED PROTEIN WITH RIBONUCLEASE H-LIKE AND RETROVIRUS ZINC FINGER-LIKE DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22936">
        <property type="entry name" value="Pol_BBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25597">
        <property type="entry name" value="SH3_retrovirus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="3">
        <location>
            <begin position="474"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="197"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="254"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="197"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="258"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9692786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="694" mass="78053" checksum="14B1358E466FA501" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DRC2</accession>
    <name>A0AA39DRC2_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_010063</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ARG7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DRC2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009733">
        <property type="term" value="P:response to auxin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003676">
        <property type="entry name" value="SAUR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31374">
        <property type="entry name" value="AUXIN-INDUCED PROTEIN-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31374:SF148">
        <property type="entry name" value="AUXIN-RESPONSIVE PROTEIN SAUR36-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02519">
        <property type="entry name" value="Auxin_inducible"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9694391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="128" mass="14328" checksum="4630E6AB9271BE75" modified="2024-01-24" version="1">MRRRGGALVLRLVRKMLTRVFTFSRRSNFTVRAAAAAAAVDDDCDDDMGPLPEDVKEGHFVVQTIYDGELKRFVIELGYLSHPGFLKLLELAEEEFGFEQEGVLAVPCGPSELLRILETRKEGSGDNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DT65</accession>
    <name>A0AA39DT65_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Brix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_008641</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DT65"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007109">
        <property type="entry name" value="Brix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045112">
        <property type="entry name" value="PPAN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12661">
        <property type="entry name" value="PETER PAN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12661:SF5">
        <property type="entry name" value="SUPPRESSOR OF SWI4 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04427">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00879">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50833">
        <property type="entry name" value="BRIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Brix" evidence="2">
        <location>
            <begin position="32"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="305"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="305"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="316"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9696521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="330" mass="37052" checksum="F664D779D69074AB" modified="2024-01-24" version="1">MARFRNNKKKGFVKPVVKKQPGVDHVTGEKIPHSIVFSRGKIPSSLKQLQMDLRKLMLPFTAVNLKEKRRNNLKDFLNVAGPLGVTHFLILSKTETAPYLKVARTPQGPTLTFKIHEYSLAVDVAQSQLRPRCPQDLFKNSPLIVLSGFGSGEQHLKLTTVMFQNIFPAIDVNTVKLSSCQRIVLLNYNKDTKFIDFRHYSIRLQPVGVSKRVRKFVQNHQVPDLKNLQDVSDFITKAGYGSESEVDDEAATVTLTSDLGRINKASSKSAVKLQEIGPRMTLQLIKIEEGLCSGGVIFNAYGNEAEKKKEVKEEEDKINEEDADEDADED</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DTH4</accession>
    <name>A0AA39DTH4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_010479</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DTH4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05476">
        <property type="entry name" value="pepsin_A_like_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000120">
        <property type="entry name" value="Aspartic proteinase nepenthesin-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000034">
        <property type="entry name" value="Aspartyl protease family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034161">
        <property type="entry name" value="Pepsin-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051708">
        <property type="entry name" value="Plant_Aspart_Prot_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032799">
        <property type="entry name" value="TAXi_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032861">
        <property type="entry name" value="TAXi_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47967">
        <property type="entry name" value="OS07G0603500 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47967:SF36">
        <property type="entry name" value="PEPTIDASE A1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14541">
        <property type="entry name" value="TAXi_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14543">
        <property type="entry name" value="TAXi_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase A1" evidence="8">
        <location>
            <begin position="95"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="7">
        <location>
            <begin position="165"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="113"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9695005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="474" mass="51832" checksum="EBD656DEDF7E3B0E" modified="2024-01-24" version="1">MRLEKSMASSSSIILILCGFTLFSILLLASSSPDKNPTTITLPLTPLFTKNPSSDPWQLLSHLTSASLARAHHLKHRKNTSSVNIPLFAHSYGGYSVSLSFGTPPQTLSFVMDTGSSLVWFPCTSRYVCASCSFPNIDPAKIPTFIPKLSSSAKILGCLNPKCGFVTDSDVRTRCPGCDQNSANCTKACPTYAIQYGLGSTVGLLLLESLVFAERTEPDFVVGCSILSYRQPSGIAGFGRGPSSLPKQMGLKKFSYCLLSHRFDDSPESSKMTLYVGPDSKDDKTGGLRYTPFRKNPVSSNFAFEEYYYVTLRHIIVGDKRVKIPFRFMVPGSDGNGGTIVDSGSTYTFMEKPVFEAVAAEFDRQMANYTRAADLEARSGLKPCFNLSGVDSLAFPSLVFQFKGGAKMELPVANYFSLVGDLSVLCLTIVSNEAVASTLSSGPSIILGNYQSQNFYTEYDLENERFGFRRQRCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DTM4</accession>
    <name>A0AA39DTM4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Beta-glucosidase 11-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_008638</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the glycosyl hydrolase 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DTM4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000069">
        <property type="entry name" value="Beta-glucosidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033132">
        <property type="entry name" value="GH_1_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001360">
        <property type="entry name" value="Glyco_hydro_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353:SF29">
        <property type="entry name" value="BETA-GLUCOSIDASE 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353">
        <property type="entry name" value="GLYCOSYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00232">
        <property type="entry name" value="Glyco_hydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00131">
        <property type="entry name" value="GLHYDRLASE1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00653">
        <property type="entry name" value="GLYCOSYL_HYDROL_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041384283" description="Beta-glucosidase 11-like" evidence="6">
        <location>
            <begin position="23"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9696518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="513" mass="58417" checksum="6B4A1D38D3345A23" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DU89</accession>
    <name>A0AA39DU89_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_009088</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DU89"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF78">
        <property type="entry name" value="OS12G0587133 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="483"/>
            <end position="763"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9697163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1215" mass="139542" checksum="93686522CE419C18" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DV09</accession>
    <name>A0AA39DV09_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Proline-rich protein 3-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_008669</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DV09"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33470">
        <property type="entry name" value="OS01G0164075 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33470:SF40">
        <property type="entry name" value="PROTEIN SEED AND ROOT HAIR PROTECTIVE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01190">
        <property type="entry name" value="Pollen_Ole_e_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041415898" description="Proline-rich protein 3-like" evidence="3">
        <location>
            <begin position="23"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="30"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="32"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="133"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="167"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9696565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="331" mass="36954" checksum="495D8CA78472700E" modified="2024-01-24" version="1">MALTRFFTTSMILFSLLAIALASNGGYGPNPELEKPKPEYNKGYDVKPAVEKPEPDYNKGYGSAPEAETPKPEYKKGYDLKPVMEKPDPEYEEGYVSKTEVEKPKPEYNNGYVPKPEIEKPKPEYNNGYVPKPEVEKPKPEYQKEYGPKPEVEKPKPEYNKGYGSKPEVEKPKPEYNKGYGPKPEVEKPKPEYEKKLLPTAIGVQGLIYCKSGLKTVPLPGAVARITCLAADEQGYEMAPFSFLSGPSDEKGYYFATLYPSQVKDNCKLTQCKAFLENSPLKTCNVPTDENKGIDGAPVVPSSNLKAKKMQLYSVKPLFYTSQPEPVSNGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DV12</accession>
    <name>A0AA39DV12_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">cytokinin dehydrogenase</fullName>
            <ecNumber evidence="3">1.5.99.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_009089</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>N(6)-dimethylallyladenine + A + H2O = 3-methyl-2-butenal + adenine + AH2</text>
            <dbReference type="Rhea" id="RHEA:13625"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15825"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:17660"/>
            <dbReference type="EC" id="1.5.99.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oxygen-dependent FAD-linked oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.5.99.12" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DV12"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019139">
        <property type="term" value="F:cytokinin dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009690">
        <property type="term" value="P:cytokinin metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.462.10">
        <property type="entry name" value="FAD-linked oxidases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="entry name" value="Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016170">
        <property type="entry name" value="Cytok_DH_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015345">
        <property type="entry name" value="Cytokinin_DH_FAD/cytokin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016164">
        <property type="entry name" value="FAD-linked_Oxase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050432">
        <property type="entry name" value="FAD-linked_Oxidoreductases_BP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006093">
        <property type="entry name" value="Oxy_OxRdtase_FAD_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13878:SF127">
        <property type="entry name" value="CYTOKININ DEHYDROGENASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13878">
        <property type="entry name" value="GULONOLACTONE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09265">
        <property type="entry name" value="Cytokin-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55103">
        <property type="entry name" value="FAD-linked oxidases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00862">
        <property type="entry name" value="OX2_COVAL_FAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041237033" description="cytokinin dehydrogenase" evidence="8">
        <location>
            <begin position="24"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="9">
        <location>
            <begin position="65"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9697164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="522" mass="58689" checksum="87C5E00A161FDC6E" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DV35</accession>
    <name>A0AA39DV35_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">small monomeric GTPase</fullName>
            <ecNumber evidence="3">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PVL29_008717</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Small GTPase that cycles between an active GTP-bound and an inactive GDP-bound state and mainly functions in vesicle-mediated endoplasmic reticulum (ER) to Golgi transport. The active GTP-bound form inserts into the endoplasmic reticulum membrane where it recruits the remainder of the coat protein complex II/COPII. The coat protein complex II assembling and polymerizing on endoplasmic reticulum membrane is responsible for both the sorting of cargos and the deformation and budding of membranes into vesicles destined to the Golgi.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Golgi apparatus</location>
            <location evidence="11">Golgi stack membrane</location>
            <topology evidence="11">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 18">Belongs to the small GTPase superfamily. SAR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DV35"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00879">
        <property type="entry name" value="Sar1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000261">
        <property type="entry name" value="GTP-binding protein SAR1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006687">
        <property type="entry name" value="Small_GTPase_SAR1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45684">
        <property type="entry name" value="RE74312P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51422">
        <property type="entry name" value="SAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 18" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 18" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="9 18" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 16" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 18" id="KW-0653">Protein transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 18" id="KW-0813">Transport</keyword>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="27"/>
            <end position="34"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="129"/>
            <end position="132"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00057753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR606687-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR606687-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU003926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ9696640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="193" mass="22007" checksum="5E729B7A5DA15A1D" modified="2024-01-24" version="1">MFLLDWFYGVLASLGLWQKEAKILFLGLDNAGKTTLLHMLKDERLVQHQPTQYPTSEELSIGKIKFKAFDLGGHQIARRVWKDYYAKVDAVVYLVDAYDKDRFAESKKELDALLSDEALANVPFLVLGNKIDIPYAASEDELRHHLGLTNFTTGKGRVNLADSNVRPLEVFMCSIVRKMGYGDGFKWLSQYIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DVF7</accession>
    <name>A0AA39DVF7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_009421</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 3">Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the peptidase C19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DVF7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02674">
        <property type="entry name" value="Peptidase_C19R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2230.10">
        <property type="entry name" value="DUSP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035927">
        <property type="entry name" value="DUSP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006615">
        <property type="entry name" value="Pept_C19_DUSP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057372">
        <property type="entry name" value="Ubiquitin_UBP8/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF18">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06337">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25242">
        <property type="entry name" value="Ubiquitin_UBP8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00695">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143791">
        <property type="entry name" value="DUSP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51283">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="DUSP" evidence="5">
        <location>
            <begin position="15"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="4">
        <location>
            <begin position="326"/>
            <end position="931"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00037450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9697584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="955" mass="107273" checksum="EF62F3BC7FCEE286" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DW93</accession>
    <name>A0AA39DW93_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_007487</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DW93"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000146">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase IOS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000228">
        <property type="entry name" value="Serine/threonine-protein kinase BIK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050823">
        <property type="entry name" value="Plant_Ser_Thr_Prot_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45621">
        <property type="entry name" value="OS01G0588500 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="85"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9698424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="443" mass="50294" checksum="F607F1E0C46D50CE" modified="2024-01-24" version="1">MKCFHFNNGERREEEDGVLSRASKVSWARSLSVASSSADVRRSEFDFDSRDLSDSVSFHEFLVQRRANDLRVFTFSELKSATRGFSRALMIGEGGFGCVYRGVVRVHADEAPDSKMDVAVKQLNRNGFQGHKEWINEVNFLGVVKHPNLVKLVGYCAEDDERGIQRLLVYELMCNKSLEDHLLARVPSSLPWMTRLKIAQDSARGLAYLHEEMDFQLIFRDFKTSNVLLDEDFNAKLSDFGLARQGPSQGVSHVSTSVVGTVGYAAPEYVHTGRLTAKSDVWSFGVVLYELITGRRAVERNLPRSEQKLLEWVRPYVSDSKKFHLIVDPRLEGEYCIKSAQKLASLANKCLSKQPKSRPKMSEVVEILGNIISEIAPQEEVAPQPINETDNVKEEAVEETEPIKQGNNYLRKVFDLKDMVNLRTKSVGKLDWRNWTPGMVRTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E1E9</accession>
    <name>A0AA39E1E9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein LAZ1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_002937</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E1E9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005178">
        <property type="entry name" value="Ostalpha/TMEM184C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23423">
        <property type="entry name" value="ORGANIC SOLUTE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03619">
        <property type="entry name" value="Solute_trans_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01417">
        <property type="entry name" value="Solute_trans_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="164"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="198"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="237"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="277"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="420"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="372"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="437"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9704618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="467" mass="52651" checksum="44A964E4B93CF2C9" modified="2024-01-24" version="1">MKLTGFLDINPLGSSTPTWASLVAGVFVLITLSLSMYLLFEHLSSYKNPEEQKFLIGVILMVPCYAIESFVSLVNPSISVDCAILRDCYEAFAMYCFGRYLVACLGGEERTIEFMERQGRASSKTPLLENNCEKGTVKHPFPMNYFLKPWKLGQWFYQVIKIGIVQYMIIKSLSAILAVILEAFSLYCEGDFKWGCGYPYIAVVLNFSQSWALYCLVQFYTVTKDELEHIKPLAKFLTFKSIVFLTWWQGVAIALLYDLGLFKSAIAQGLQLKSSVQDFIICIEMGVASIVHLYVFPAKPYELMGDRLSGSVSVRDSERPTKLRLPHPDIDIRSGMTIRESVRDVFIGGGGYIVNDVKFTVNQAVEPVEKGITKFNQKLHKISQNIKRHDKEKRRTKDDSCITPTRRVIRGIDDPLLNGSFSDSGVSRGKKHRRKSGYTSGESGGESSSDQSYSAYQIRGGRWVTKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E3G3</accession>
    <name>A0AA39E3G3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">AP complex subunit sigma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_005129</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <location evidence="2">Coated pit</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the adaptor complexes small subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E3G3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030122">
        <property type="term" value="C:AP-2 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035615">
        <property type="term" value="F:clathrin adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14833">
        <property type="entry name" value="AP2_sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000004">
        <property type="entry name" value="AP complex subunit sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016635">
        <property type="entry name" value="AP_complex_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027156">
        <property type="entry name" value="APS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11753">
        <property type="entry name" value="ADAPTOR COMPLEXES SMALL SUBUNIT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015588">
        <property type="entry name" value="AP_complex_sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0168">Coated pit</keyword>
    <keyword evidence="6" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AP complex mu/sigma subunit" evidence="11">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR015588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9703702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="142" mass="17105" checksum="FC6768247EE9991E" modified="2024-01-24" version="1">MIRFILLQNRQGKTRLAKYYVPFEESEKHKVEYEVHRLVVNRDPKHTNFVEFRTHKVIYRRYAGLFFSLCVDITDNELAYLECIHLFVEILDHFFSNVCELDLVFNFHKVYLILDEFILAGELQETSKKAIIERMGELEKQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E3N5</accession>
    <name>A0AA39E3N5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">RING-CH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_000404</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E3N5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16495">
        <property type="entry name" value="RING_CH-C4HC3_MARCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000337">
        <property type="entry name" value="Zinc finger family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022143">
        <property type="entry name" value="DUF3675"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033275">
        <property type="entry name" value="MARCH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23012">
        <property type="entry name" value="RING/FYVE/PHD ZINC FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23012:SF93">
        <property type="entry name" value="RING_FYVE_PHD ZINC FINGER SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12428">
        <property type="entry name" value="DUF3675"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="179"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="203"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="RING-CH-type" evidence="8">
        <location>
            <begin position="54"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="62"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="237"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9708341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="267" mass="30453" checksum="D8E97B0D17D9DB2D" modified="2024-01-24" version="1">MGDHFVLLVDRLLTESTLEAAIESRNRLMQASVSAIADTKVDCKSHKMDFKHGSSPRKLVECRICQDEDEDSNMETPCSCCGSLKYAHRSCVQRWCNEKGNTMCEICQQQFKPGYTAPPPLFQFRSIPINLRGNWEISRGELNNPRFIAMVSTDRNFLDPDYNEFSTSTSRSMMCCRSVAIIFMVLLILRHTLPVMINGAEGYTFPLFMLVLLRTAGIILPMYIMLRAVTAIQRRRRQQDPPTPYASTDEETEQPALQPQTHIIHVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E4C6</accession>
    <name>A0AA39E4C6_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_000842</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E4C6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9709061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="98" mass="10055" checksum="3E3CB9C86744ED8A" modified="2024-01-24" version="1">MSMIIGSLRQVIESHRHSPIESIGNMASWNKAATLIACFAILVVVAAAAAKEDMPGMKMGPSPAPSPSDAGRTITSSSALLTGLLAFIVSFLAVRGSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E4Y7</accession>
    <name>A0AA39E4Y7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Interferon-related developmental regulator N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_003341</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IFRD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E4Y7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039777">
        <property type="entry name" value="IFRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007701">
        <property type="entry name" value="Interferon-rel_develop_reg_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12354">
        <property type="entry name" value="INTERFERON-RELATED DEVELOPMENTAL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12354:SF1">
        <property type="entry name" value="INTERFERON-RELATED DEVELOPMENTAL REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05004">
        <property type="entry name" value="IFRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Interferon-related developmental regulator N-terminal" evidence="2">
        <location>
            <begin position="7"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9705232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="405" mass="46064" checksum="C260A75E2575C28F" modified="2024-01-24" version="1">MRILPQLRHSWGSVREEALCSIITGSTVKVQVQFAENNFVTLVYRSLALLKKGSPKEVSLATQAIALFAITLGPGGHTHEIYKDSLPPLSQALESGSESSKISSILDCLAIVTFVGAKDFEETEKSMQIIWKLIQPVALNVNARKHSPAILAAAISAWSFILTTVDGWRLNYKYCRGAISYLSDLIEYDDDSVRIAALEALALIFESGCFNSFSTEAKCSSSCLIHEGNTSTNGYSPIQEMKDNILHRVRRLSLKDEVDKDHMSDWLKVFEDGCCPKTLVMNWQNMLKSFAWYQLLQIKFMKRILGSGFNKHIQENEFLRELFEIVTPKREDVSGYGCQLYELDSKEVIAEYYEPELRKNNKSSIQRMFMSPNSFYNKAMTQLLNKRRMLSQKRRNGHYVVDDEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E4Z3</accession>
    <name>A0AA39E4Z3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GH10 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_003352</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 10 (cellulase F) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E4Z3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031176">
        <property type="term" value="F:endo-1,4-beta-xylanase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044846">
        <property type="entry name" value="GH10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001000">
        <property type="entry name" value="GH10_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31490">
        <property type="entry name" value="GLYCOSYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31490:SF2">
        <property type="entry name" value="GLYCOSYL HYDROLASE FAMILY 10 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00331">
        <property type="entry name" value="Glyco_hydro_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00633">
        <property type="entry name" value="Glyco_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51760">
        <property type="entry name" value="GH10_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GH10" evidence="5">
        <location>
            <begin position="196"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9705247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="535" mass="59581" checksum="05A85CABAEFE240A" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E500</accession>
    <name>A0AA39E500_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase</fullName>
            <ecNumber evidence="3">3.2.2.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_002335</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>NAD(+) + H2O = ADP-D-ribose + nicotinamide + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16301"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57967"/>
            <dbReference type="EC" id="3.2.2.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:16302"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the disease resistance TIR-NB-LRR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.6" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E500"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061809">
        <property type="term" value="F:NAD+ nucleosidase activity, cyclic ADP-ribose generating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050832">
        <property type="term" value="P:defense response to fungus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043068">
        <property type="term" value="P:positive regulation of programmed cell death"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10140:FF:000007">
        <property type="entry name" value="Disease resistance protein (TIR-NBS-LRR class)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32009:SF39">
        <property type="entry name" value="TIR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32009">
        <property type="entry name" value="TMV RESISTANCE PROTEIN N-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TIR" evidence="10">
        <location>
            <begin position="11"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9707304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="215" mass="24416" checksum="B2740BF39FA262AB" modified="2024-01-24" version="1">MAAPSFASSQWSYDAFLCFRGEDTRFSFAAHLYYALQRKGVNTFFDDHKIRRGESISPTLVRAIEGSRSSIIILSQNYASSTSCLEELVKILECRITLGQLVLPIFYNVDPSDVRKHKRSFGEALDEHEKTFKHDMDKVKNWRVALSEVANLAGWNSRKKEIVFDVLKELFGLFPGDAKRLVEEDSPIQKTEVVPHSITVHTAGHFGTRVLHALK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YHT6</accession>
    <name>A0AA38YHT6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PI-PLC X domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_026939</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YHT6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08588">
        <property type="entry name" value="PI-PLCc_At5g67130_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.190:FF:000048">
        <property type="entry name" value="PI-PLC X domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051057">
        <property type="entry name" value="PI-PLC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593:SF140">
        <property type="entry name" value="PLC-LIKE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26178">
        <property type="entry name" value="PI-PLC_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="408"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9670714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="428" mass="47132" checksum="153CBC7F5FCCFC9D" modified="2024-01-24" version="1">MFPSFSDQRSLCRASATPNGYFLLLLIISVLIINSAACSNRNCQVLDPCSLATDCGPGLYCGNCPAMGKNQPICTRGQATIPTTIINGLPFNKYSWLMTHNAFSIVDAPPLPGVQRITFYNQEDTVTNQLRNGVRGLMLDMYDFENDIWLCHSFRGQCYNFTAFQPAINTLKEVETFLSENPSEIVTIVIEDYVHTPKGLTNLFTNAGLVKYWFPVSKMPRKGEDWPTMTDMVQENHRLLVFTSIASKEADEGIAYQWRYMVENESGDGGVVQGSCPNRKESQPLNSKSASLFLQNSFPSFPVESDSCKEHSATLGELVGTCYKAAGNVMPNFLAVNFYMRSDGGGVFDVLDRMNGQTLCGCSTVSACQAGAPFGTCKNVAVSNTTQTPNTFGSFSGSVQLSGSASTIHFMNLLYFFLLFFSFLVFQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YJ92</accession>
    <name>A0AA38YJ92_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 13">Uroporphyrinogen decarboxylase</fullName>
            <ecNumber evidence="7 13">4.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_025250</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the decarboxylation of four acetate groups of uroporphyrinogen-III to yield coproporphyrinogen-III.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>uroporphyrinogen III + 4 H(+) = coproporphyrinogen III + 4 CO2</text>
            <dbReference type="Rhea" id="RHEA:19865"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57308"/>
            <dbReference type="ChEBI" id="CHEBI:57309"/>
            <dbReference type="EC" id="4.1.1.37"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Porphyrin-containing compound metabolism; chlorophyll biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 13">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 4/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 14">Belongs to the uroporphyrinogen decarboxylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.37" evidence="7 13"/>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YJ92"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004853">
        <property type="term" value="F:uroporphyrinogen decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015995">
        <property type="term" value="P:chlorophyll biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00717">
        <property type="entry name" value="URO-D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.210:FF:000006">
        <property type="entry name" value="Uroporphyrinogen decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00218">
        <property type="entry name" value="URO_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038071">
        <property type="entry name" value="UROD/MetE-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006361">
        <property type="entry name" value="Uroporphyrinogen_deCO2ase_HemE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000257">
        <property type="entry name" value="Uroporphyrinogen_deCOase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01464">
        <property type="entry name" value="hemE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21091">
        <property type="entry name" value="METHYLTETRAHYDROFOLATE:HOMOCYSTEINE METHYLTRANSFERASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21091:SF167">
        <property type="entry name" value="UROPORPHYRINOGEN DECARBOXYLASE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01208">
        <property type="entry name" value="URO-D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51726">
        <property type="entry name" value="UROD/MetE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00906">
        <property type="entry name" value="UROD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00907">
        <property type="entry name" value="UROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0149">Chlorophyll biosynthesis</keyword>
    <keyword evidence="8 13" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="10 13" id="KW-0456">Lyase</keyword>
    <keyword evidence="11 13" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Uroporphyrinogen decarboxylase (URO-D)" evidence="15">
        <location>
            <begin position="69"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="Uroporphyrinogen decarboxylase (URO-D)" evidence="16">
        <location>
            <begin position="188"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS00906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS00907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9671447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="409" mass="45168" checksum="29973F75CEB4C0B8" modified="2024-01-24" version="1">MSLSSPTSMCCCLGWSSSTSVQLGFLPARFFRVSSRRTKRFHFKCSSSVPDSDPLLVKAARGDPVTRPPAWMMRQAGRYMAVYRKLAEKYPSFRQRSETTDLIVEISLQPWEAFHPDGVIIFSDILTPLPAFGVPFDIEEVRGPVIHSPIRSEEGLKVLHPIDLKKLHFVGESLRILRQEVGGQAAVLGFVGAPWTIGTYIVEGGTTRTYTIIKSMCHTAPNVLRALLSHLTQAISEYIVFQVESGAHCIQIFDSWGGQLPPDMWECWSKPYIEEMVSVVRKRCPGTPIVLYINGNGGLLERMKGTGVDVIGLDWTVDMADGRKRLGSGISIQGNVDPAYLFSPLPALTDEIQRVVRCAGPRGHILNLGHGVLVGTPEEAVAHFFEVARNLNLSTLLEDNAVEEPKLAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YKH2</accession>
    <name>A0AA38YKH2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ataxin 2 SM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_025665</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YKH2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034063">
        <property type="term" value="P:stress granule assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045117">
        <property type="entry name" value="ATXN2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025852">
        <property type="entry name" value="SM_dom_ATX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12854">
        <property type="entry name" value="ATAXIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12854:SF12">
        <property type="entry name" value="POLYADENYLATE-BINDING PROTEIN INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14438">
        <property type="entry name" value="SM-ATX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ataxin 2 SM" evidence="2">
        <location>
            <begin position="19"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="209"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="222"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9672108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="575" mass="61072" checksum="0FD19309BC903964" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YN47</accession>
    <name>A0AA38YN47_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein phosphatase</fullName>
            <ecNumber evidence="1">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_023197</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 6">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PP2C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="1"/>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YN47"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039123">
        <property type="entry name" value="PPTC7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12320:SF14">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12320">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0464">Manganese</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="2">
        <location>
            <begin position="15"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU366020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9673490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="259" mass="28515" checksum="293E4077C08C28A6" modified="2024-01-24" version="1">MKILSERSLMMDLGSFYIPDKSKSSPMGDDAHFISKHHQTIGLADGVAGWAKQGIDGGEYARQLMDNCVTTLYAEDKEIVYPQMVLEEAYVNTNVEGSSTACIITLTDEYLNVINVGDSGFMLFRNGNMIYKSSIQQHFFNCPCQLGKSSRCDDPSIAEELILGVRAGDVVVVGTDGLFDNVFEREMEMVIRVLREEGCLEPQLLAKLLAELALENSLIRSGDSPYTIAALMEGKFRSGGKPDDITVIVARIVPPMDIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YN72</accession>
    <name>A0AA38YN72_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_023257</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YN72"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF78">
        <property type="entry name" value="OS12G0587133 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase zinc-binding" evidence="1">
        <location>
            <begin position="377"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9673579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="522" mass="59978" checksum="A10933210A9BC8EE" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YP96</accession>
    <name>A0AA38YP96_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_023508</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YP96"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044659">
        <property type="entry name" value="PELPK1_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33088">
        <property type="entry name" value="MUCIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33088:SF28">
        <property type="entry name" value="PROTEIN PELPK1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041406551" evidence="2">
        <location>
            <begin position="29"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="129"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9673994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="151" mass="16436" checksum="8029B864F1D93F96" modified="2024-01-24" version="1">MAHHHEPSILLPLLLITLSLMSCKEVFASRHLLEETALPKVPELPKPELPPLPTLPTFPKPELPPLPHVPTLPESTLPTLPKPELPTVPHVPALEKPELSPLPKLEVPKLPESPSLPHLPDLPKPTLPTVPTLPKDIPFPSLSPPHSTTSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YPK8</accession>
    <name>A0AA38YPK8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_023651</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YPK8"/>
    <dbReference type="SMR" id="A0AA38YPK8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000042">
        <property type="entry name" value="probable strigolactone esterase DAD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43039">
        <property type="entry name" value="ESTERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="20"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9674222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="266" mass="29387" checksum="274510749976671A" modified="2024-01-24" version="1">MGNTLLEALNVRVVGNGERVLVLAHGFGTDQSAWQRILPYFLPHFRIILYDLVCAGSVNPDYFDFRRYTTLDAFVDDLLNILDALGVDRCAYVGHSISGMIGILAAIRRPELFTKLVLIGASPRFLNDHDYHGGFEEGEIEKVFSAMEANYDAWVHGFAPLAVGADVPAAVREFSRTLFNMRPDITLFVSQTIFNSDFRGVLGLVKVPCCIIQTAKDASVPTSVAVYLKNHLGGRNTVEMLNVEGHLPHLSAPMLLAPVLRRALSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YQ45</accession>
    <name>A0AA38YQ45_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_023874</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YQ45"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33918:SF4">
        <property type="entry name" value="ABC-2 TYPE TRANSPORTER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33918">
        <property type="entry name" value="OS01G0704200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="167"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="213"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="245"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="320"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9674598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="355" mass="39504" checksum="00C8291E54E9CCDA" modified="2024-01-24" version="1">MELQSISCWLPSPDMRLCIHRPLSHRIVSTGHVRWKDQRFRFEKVSKSYLMPGLKKECSLSSFGRGATLCSLNSNSDAKLKYSGREITSVTGNGFDGVEPFRGKSGSVSFHGLTHQMVEESKLASSPFKEGTGSFLWVLAPVALISSLVLPQFFLDSAIEAILKDEVLVEIVATIFSEAMFYIGLATFLHVTDHVQRPYLQFSPKRWGLITGLKGYLTTAFFTMGFKIFAPLFAVYVTWPVLGPSALVAVAPFLVGCAAQLAFEMRLSKRGSSCWPLVPIIFEVYRLYQLSKAAHFMDILVLAMKEAPVTPDMVERGGALVAMVVTFQVLGLVCLWSLMTFLMRLFPSRPVAENY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YR08</accession>
    <name>A0AA38YR08_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_024127</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YR08"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37614">
        <property type="entry name" value="OS02G0121400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37614:SF2">
        <property type="entry name" value="OS02G0121400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9675046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="173" mass="19550" checksum="F951405647CCC21F" modified="2024-01-24" version="1">MKRKNHNCFVDDDEFEVAHVLKKEDLLKMVGSLTHQRELLKSEIQNVKLLCNTLKALNPELKTRKQHLSLDMTIVKARVESAEQVYLQQPLPLLRHSGQTSALVPFTVGSDVVYPILGPDLSPSLDLNVNIALNFDTTQLKRIAIAQDRKKRLQIYKVKKPKDGPSPSILSFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YRX8</accession>
    <name>A0AA38YRX8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C-JID domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_024428</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YRX8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045344">
        <property type="entry name" value="C-JID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050715">
        <property type="entry name" value="LRR-SigEffector_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45752:SF195">
        <property type="entry name" value="LEUCINE-RICH REPEAT (LRR) FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45752">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20160">
        <property type="entry name" value="C-JID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="C-JID" evidence="3">
        <location>
            <begin position="203"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9675500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="346" mass="39009" checksum="584B7E026AF7AC04" modified="2024-01-24" version="1">MCSKLQQLPENLGGLQSLQVLYAAFLDSISFQLLSLSGLCSLRILVLQKTDVMQGAIPSDICCLSSLEQLNLSNCNLIEGGIPSEIYNLSSLRALSLGENHFSSIPAGINKLSQLRVLNLRHCQRLLQIPELPSSLRLLDVHGCTLLETLSSPSSLLPSCLFKCFESTIQDSECGINNWMFTYDHGYCYSQELKIFISSSGKPGWTRYQNEESMVSIELPWNWYRNNDLLGFALCSVYVPPDKESEDGHQDGDDWCLECELTIGGGHQFNLVDRFLFQCRWCDCRIDDVSSQVLVRYYPKIRIPSEYASNKSLKAAFRGYFKGKPVEVENCGIHLIYANEQEPSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YTD6</accession>
    <name>A0AA38YTD6_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_024985</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9676250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YTD6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010333">
        <property type="term" value="F:terpene synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016102">
        <property type="term" value="P:diterpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00684">
        <property type="entry name" value="Terpene_cyclase_plant_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.600.10:FF:000007">
        <property type="entry name" value="Isoprene synthase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.130:FF:000001">
        <property type="entry name" value="Isoprene synthase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.130">
        <property type="entry name" value="Terpene synthase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034741">
        <property type="entry name" value="Terpene_cyclase-like_1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044814">
        <property type="entry name" value="Terpene_cyclase_plant_C1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001906">
        <property type="entry name" value="Terpene_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036965">
        <property type="entry name" value="Terpene_synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050148">
        <property type="entry name" value="Terpene_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005630">
        <property type="entry name" value="Terpene_synthase_metal-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225">
        <property type="entry name" value="OS04G0344100 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225:SF241">
        <property type="entry name" value="TERPENE SYNTHASE FAMILY, METAL-BINDING DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01397">
        <property type="entry name" value="Terpene_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03936">
        <property type="entry name" value="Terpene_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01019">
        <property type="entry name" value="Terpene_Cyclase_Like_1_C_Termi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Terpene synthase N-terminal" evidence="5">
        <location>
            <begin position="29"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="Terpene synthase metal-binding" evidence="6">
        <location>
            <begin position="247"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9676250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="535" mass="62649" checksum="763769E7A558689E" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YYL4</accession>
    <name>A0AA38YYL4_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_020945</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YYL4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9678897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="118" mass="12853" checksum="78A228A245069C72" modified="2024-01-24" version="1">MATLLVTFSPGNAAAAHGLHGAVATAMPCPLLVSVPRMDPSSQWYFGTVIDIGRVNIVSNYGMPDSADAYPHGVQKIFEVNIKELPEQILIPLCACHRNGKWPAPPSPWVAFERFKRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YYS0</accession>
    <name>A0AA38YYS0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cucumisin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_021195</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the peptidase S8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YYS0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02120">
        <property type="entry name" value="PA_subtilisin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04852">
        <property type="entry name" value="Peptidases_S8_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034197">
        <property type="entry name" value="Peptidases_S8_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045051">
        <property type="entry name" value="SBT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041469">
        <property type="entry name" value="Subtilisin-like_FN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10795">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17766">
        <property type="entry name" value="fn3_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 7" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Peptidase S8/S53" evidence="9">
        <location>
            <begin position="37"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="Subtilisin-like protease fibronectin type-III" evidence="10">
        <location>
            <begin position="526"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="635"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6 7">
        <location>
            <position position="45"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6 7">
        <location>
            <position position="108"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6 7">
        <location>
            <position position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9679188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="670" mass="72554" checksum="37C3BD8AD59FF8ED" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YZM7</accession>
    <name>A0AA38YZM7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_021539</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YZM7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000111">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:002727">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046956">
        <property type="entry name" value="RLP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063">
        <property type="entry name" value="LRR RECEPTOR-LIKE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063:SF101">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE FLS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041291675" description="Leucine-rich repeat-containing N-terminal plant-type domain-containing protein" evidence="13">
        <location>
            <begin position="29"/>
            <end position="1136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1082"/>
            <end position="1106"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="14">
        <location>
            <begin position="35"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="15">
        <location>
            <begin position="383"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9679641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1136" mass="126364" checksum="D1AC7D9F53A5E821" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z024</accession>
    <name>A0AA38Z024_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 20">Pyruvate kinase</fullName>
            <ecNumber evidence="6 20">2.7.1.40</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">PVL29_021542</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="23">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19 20">
            <text>pyruvate + ATP = phosphoenolpyruvate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18157"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.40"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 20">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Plastid</location>
            <location evidence="3">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 20">Belongs to the pyruvate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.40" evidence="6 20"/>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z024"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009570">
        <property type="term" value="C:chloroplast stroma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030955">
        <property type="term" value="F:potassium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004743">
        <property type="term" value="F:pyruvate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.33.10:FF:000003">
        <property type="entry name" value="Pyruvate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.60:FF:000025">
        <property type="entry name" value="Pyruvate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1380.20:FF:000008">
        <property type="entry name" value="Pyruvate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.33.10">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1380.20">
        <property type="entry name" value="Pyruvate kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001697">
        <property type="entry name" value="Pyr_Knase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011037">
        <property type="entry name" value="Pyrv_Knase-like_insert_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018209">
        <property type="entry name" value="Pyrv_Knase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015793">
        <property type="entry name" value="Pyrv_Knase_brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015795">
        <property type="entry name" value="Pyrv_Knase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036918">
        <property type="entry name" value="Pyrv_Knase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015806">
        <property type="entry name" value="Pyrv_Knase_insert_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004491">
        <property type="entry name" value="PRK05826.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01064">
        <property type="entry name" value="pyruv_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11817">
        <property type="entry name" value="PYRUVATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00224">
        <property type="entry name" value="PK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02887">
        <property type="entry name" value="PK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01050">
        <property type="entry name" value="PYRUVTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50800">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52935">
        <property type="entry name" value="PK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00110">
        <property type="entry name" value="PYRUVATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="17 20" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="12 20" id="KW-0418">Kinase</keyword>
    <keyword evidence="14 20" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0934">Plastid</keyword>
    <keyword evidence="16" id="KW-0630">Potassium</keyword>
    <keyword evidence="18" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="15" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Pyruvate kinase barrel" evidence="21">
        <location>
            <begin position="107"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate kinase C-terminal" evidence="22">
        <location>
            <begin position="468"/>
            <end position="556"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU000504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF00224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF02887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAJ9679647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="576" mass="63497" checksum="3B082BFA6631E64E" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z1N6</accession>
    <name>A0AA38Z1N6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">T-complex protein 1 subunit alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">CCT-alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_019841</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. Known to play a role, in vitro, in the folding of actin and tubulin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z1N6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03335">
        <property type="entry name" value="TCP1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000009">
        <property type="entry name" value="T-complex protein 1 subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000070">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012715">
        <property type="entry name" value="Chap_CCT_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054827">
        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02340">
        <property type="entry name" value="chap_CCT_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 10" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9680644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="550" mass="59723" checksum="1033AA885D71B8F8" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z3M8</accession>
    <name>A0AA38Z3M8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Exopolyphosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_017934</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9681796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z3M8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016462">
        <property type="term" value="F:pyrophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3210.10:FF:000025">
        <property type="entry name" value="Exopolyphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.150">
        <property type="entry name" value="Exopolyphosphatase. Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050273">
        <property type="entry name" value="GppA/Ppx_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048950">
        <property type="entry name" value="Ppx_GppA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003695">
        <property type="entry name" value="Ppx_GppA_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30005">
        <property type="entry name" value="EXOPOLYPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30005:SF0">
        <property type="entry name" value="RETROGRADE REGULATION PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02541">
        <property type="entry name" value="Ppx-GppA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21447">
        <property type="entry name" value="Ppx-GppA_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ppx/GppA phosphatase N-terminal" evidence="1">
        <location>
            <begin position="52"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="Ppx/GppA phosphatase C-terminal" evidence="2">
        <location>
            <begin position="198"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF21447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9681796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="382" mass="42943" checksum="D218F16467CF526B" modified="2024-01-24" version="1">MRSHIRSILQQCGIVEKIKHHGFEIAVGSSGTVQSIEKAIFSGFSRNVVSNDGALCGDLGRDWRFSREELRILVERLCGGGGEALERARRDRFFKRRSEFIVAGAVLLDEIFAMLDIEEMEVSGYALGEGVVAEMLLQVCDNYDFNANARWGSVIRLATRFLGKKKMGTASQCANITKVIFEGLRKCDMLAETQNQVKLPVSLNDKDLEYLEAACMLHNIGLITGEKGYHKQSCNIIMNGDHLYGYSTEEVKLIALLARHHRKKFPKPNHASLKEFSKEVKQKFKMLCAIIRVSIAVQKHRGLNIQGMEFSHSSEGFKLIVSETKDQSLLPGIVQPLAEGDEAELRKELGHFEMVFQEKLSIAVPSSSSELSEQSRHLQWMH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z3N0</accession>
    <name>A0AA38Z3N0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">S-adenosylmethionine synthase</fullName>
            <ecNumber evidence="16">2.5.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PVL29_017854</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Catalyzes the formation of S-adenosylmethionine from methionine and ATP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 16">
            <text>L-methionine + ATP + H2O = S-adenosyl-L-methionine + phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21080"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.5.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="16">Binds 1 potassium ion per subunit. The potassium ion interacts primarily with the substrate.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="16">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="16">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <text evidence="16">Binds 2 divalent ions per subunit. The metal ions interact primarily with the substrate. Can utilize magnesium, manganese or cobalt (in vitro).</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 16">Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 17">Belongs to the AdoMet synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.6" evidence="16"/>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9681664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z3N0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004478">
        <property type="term" value="F:methionine adenosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006556">
        <property type="term" value="P:S-adenosylmethionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18079">
        <property type="entry name" value="S-AdoMet_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.10:FF:000001">
        <property type="entry name" value="S-adenosylmethionine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.10:FF:000003">
        <property type="entry name" value="S-adenosylmethionine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.10:FF:000004">
        <property type="entry name" value="S-adenosylmethionine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00086">
        <property type="entry name" value="S_AdoMet_synth1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022631">
        <property type="entry name" value="ADOMET_SYNTHASE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022630">
        <property type="entry name" value="S-AdoMet_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022629">
        <property type="entry name" value="S-AdoMet_synt_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022628">
        <property type="entry name" value="S-AdoMet_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002133">
        <property type="entry name" value="S-AdoMet_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022636">
        <property type="entry name" value="S-AdoMet_synthetase_sfam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01034">
        <property type="entry name" value="metK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11964">
        <property type="entry name" value="S-ADENOSYLMETHIONINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02773">
        <property type="entry name" value="S-AdoMet_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02772">
        <property type="entry name" value="S-AdoMet_synt_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00438">
        <property type="entry name" value="S-AdoMet_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000497">
        <property type="entry name" value="MAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55973">
        <property type="entry name" value="S-adenosylmethionine synthetase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00376">
        <property type="entry name" value="ADOMET_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00377">
        <property type="entry name" value="ADOMET_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0170">Cobalt</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 16" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 16" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="13 16" id="KW-0630">Potassium</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 16" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="S-adenosylmethionine synthetase N-terminal" evidence="18">
        <location>
            <begin position="4"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="S-adenosylmethionine synthetase central" evidence="19">
        <location>
            <begin position="117"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="S-adenosylmethionine synthetase C-terminal" evidence="20">
        <location>
            <begin position="240"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU004462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF02773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAJ9681664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="393" mass="42977" checksum="66753F7AFE41288E" modified="2024-01-24" version="1">MDTFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNLVMIFGEITTKANVDYEKIVRDTCRTVGFVSEDVGLDSDNCKVLVNIEQQSPDIAQGVHGHLTKRPEEIGAGDQGHMFGYATDETPELMPLSHVLATKLGARLTEVRKNGTCPWLRPDGKTQVTVEYFNDHGAMVPIRVHTVLISTQHDETVTNDEIAADLKEHVIKPIIPEKFLDEKTIFHLNPSGRFVIGGPHGDAGLTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDTYATGKIPDKEILKIVKESFDFRPGMIAINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKAEKAQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z471</accession>
    <name>A0AA38Z471_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PWWP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_018010</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9681917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z471"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05162">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052657">
        <property type="entry name" value="PDP_family_Arabidopsis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000313">
        <property type="entry name" value="PWWP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10688">
        <property type="entry name" value="PWWP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10688:SF5">
        <property type="entry name" value="PWWP DOMAIN-CONTAINING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00855">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00293">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50812">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PWWP" evidence="2">
        <location>
            <begin position="211"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="462"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="633"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="789"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="823"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1024"/>
            <end position="1098"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1185"/>
            <end position="1220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="477"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="492"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="711"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="861"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="901"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1060"/>
            <end position="1069"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1192"/>
            <end position="1220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9681917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1255" mass="137164" checksum="350CF13E4767FEE0" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2025-10-08" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z4G0</accession>
    <name>A0AA38Z4G0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_018205</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057135">
        <property type="entry name" value="At4g27190-like_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050905">
        <property type="entry name" value="Plant_NBS-LRR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463:SF198">
        <property type="entry name" value="RPP4C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23247">
        <property type="entry name" value="LRR_RPS2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="167"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="1245"/>
            <end position="1417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9682215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="2165" mass="247708" checksum="652A2E2D461DDC0B" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z4X7</accession>
    <name>A0AA38Z4X7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myb/SANT-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_018400</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z4X7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045026">
        <property type="entry name" value="LIMYB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024752">
        <property type="entry name" value="Myb/SANT-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47584">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47584:SF17">
        <property type="entry name" value="MYB_SANT-LIKE DNA-BINDING DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12776">
        <property type="entry name" value="Myb_DNA-bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb/SANT-like" evidence="2">
        <location>
            <begin position="12"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="142"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9682471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="240" mass="28180" checksum="13CA226A9064A01E" modified="2024-01-24" version="1">MSQNVEISRVNWTDPIQKKRFIDLCLQEANKDFRLGGGLKSSAWPRIAKELEKLLGKCYTLKQLKNGWDYMKRQYLIWSKMMTMTGHGYNSVTKTFDWPVEKWEEYLHKYPEAKQFHFKPLANVEELEALFGGVLGVIASEAEESSTHSTSMPSETSISLEEDEDLPRNTNDEAEEQTQEEMNRIMNVLENFEGPSVKECMKILKRLLIYEDPLYYVAINAFCKKKEYREVWMEMESDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z5R1</accession>
    <name>A0AA38Z5R1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_018756</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z5R1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000054">
        <property type="entry name" value="Alpha/beta-Hydrolases superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051044">
        <property type="entry name" value="MAG_DAG_Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11614">
        <property type="entry name" value="PHOSPHOLIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="1">
        <location>
            <begin position="80"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9682891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="347" mass="39077" checksum="A5FCED0BBC78C07D" modified="2024-01-24" version="1">MAPAAKLEGVDKELQKILDAKMDEAPARRRAREAFKEIQLGIDHLLFKTPCDGVKMEEMYVVNSRGLEIFSKSWLPANSPPKAVICFCHGYGDTCTFFVEGIARKLAVSGYGFFAMDYPGFGLSDGLHAYIPSFDELVDDVVEHYSKVKEKPEFRTLPCFLFGESMGGAVLLKVHLKQPNAWTGAILVAPMCKIADDMVPPELLKQFLICIAHFLPKKKLVPQNDLAEMAFRDSKKRRLTAYNVIAYKDKPRLRTAVELLRTTQEIERRLKEVALPLLILHGEADTVTDPSVSKALYEKASSSDKKLNLYKNAYHALLEGEPDEMIIRIFDDIISWLDGHSTKTTHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z613</accession>
    <name>A0AA38Z613_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RCC1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_018781</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z613"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051553">
        <property type="entry name" value="Ran_GTPase-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058923">
        <property type="entry name" value="RCC1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45982">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45982:SF1">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25390">
        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="6"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="RCC1-like" evidence="4">
        <location>
            <begin position="8"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="58"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="111"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="171"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="217"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="269"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="322"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9682924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="395" mass="42332" checksum="4CDF3C1193600A35" modified="2024-01-24" version="1">MATKTKAIISWGSGEDGQLGIGNNEEKEWVCAVKALAAQNVCSVVAGSRNSLAICEDGKLFTWGWNQRGTLGHPPETKTENIPSQVKALANAKIVQAAIGGWHCLAVDDQGRAYAWGGNEYGQCGEEPERKDDTGRPLRRDITIPQRCASKLSVRQVAAGGTHSVVLTREGHVWTWGQPWPPGDIKQISTPVRVQGLEKVRLIAVGAFHNLALLEDGILWAWGNNEYGQLGTGDTQPRSQPIAVQGLSDLTLVDIAAGGWHSTALTDDGEVYGWGRGEHGRLGFGDDKSSKMVPQKVQLLVGEDIVQISCGGTHSVALTRNGRMFSFGRGDHGRLGYGRKVTTGHPMEVPINLPPATNLSGSGAGGHWCSKLVACGGRHTLAIVEWLAHEPEQLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z653</accession>
    <name>A0AA38Z653_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_018790</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z653"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37383">
        <property type="entry name" value="OS01G0694200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37383:SF1">
        <property type="entry name" value="OS01G0694200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9682937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="466" mass="50765" checksum="BE07B06FD4312DD9" modified="2024-01-24" version="1">MVPVVPAVAALMIEVQACKLSLPRPSISSSPPITSLLFEPHSNSLALMHSDSSFSLYPSLSPFSPPSPQSQALTLTLVPPPSSFATFLLLQNPRPNSGAHNPRVLFVVAAPHRAGAAVLLRFYVLQKTQLFAKAEVLCTQKDLQFEPKLGVLFNANHGVSVKLGGSINIFAMYSVSNSKIWVFSVKMAGDDRDDGVLLKLMKCAVIDCGVPAFSISVSGEFLILGEENGVRVFQLRPLVKGWIRKEQRESKNLNFPNGCGSKSAGVEANMEIACNGDLEGRIDLHRVSVKRRSVRFRQDSSEGSACFVAFKGKEVEHLKSMMPPLIPVKAVSIQALSAKKFLILDSDGDVHLLCLSIHHLGSEITCHMRQFTNTMKVQKLAVLPDTSTRGRTVWISDGFYSVHMMTVSDTDTSANEDDENDSEEKLKQISVTQAIFASERIQDIIPLAANALLILGQGSLFAYAIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z738</accession>
    <name>A0AA38Z738_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Nodulin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_018996</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z738"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd17354">
        <property type="entry name" value="MFS_Mch1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000446">
        <property type="entry name" value="Nodulin family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056555">
        <property type="entry name" value="NFD4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010658">
        <property type="entry name" value="Nodulin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21576:SF73">
        <property type="entry name" value="F1C9.29 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21576">
        <property type="entry name" value="UNCHARACTERIZED NODULIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23262">
        <property type="entry name" value="NFD4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06813">
        <property type="entry name" value="Nodulin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="77"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="176"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="210"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="238"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="357"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="398"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="430"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="455"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="489"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="553"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="domain" description="Nodulin-like" evidence="6">
        <location>
            <begin position="15"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="NFD4 C-terminal" evidence="7">
        <location>
            <begin position="357"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF06813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9683233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="591" mass="65145" checksum="FAB3514F24142720" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z7I4</accession>
    <name>A0AA38Z7I4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_019307</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant LTP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z7I4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01960">
        <property type="entry name" value="nsLTP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.110.10">
        <property type="entry name" value="Plant lipid-transfer and hydrophobic proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036312">
        <property type="entry name" value="Bifun_inhib/LTP/seed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016140">
        <property type="entry name" value="Bifunc_inhib/LTP/seed_store"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000528">
        <property type="entry name" value="Plant_nsLTP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33076">
        <property type="entry name" value="NON-SPECIFIC LIPID-TRANSFER PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00234">
        <property type="entry name" value="Tryp_alpha_amyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47699">
        <property type="entry name" value="Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00597">
        <property type="entry name" value="PLANT_LTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041335385" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein" evidence="2">
        <location>
            <begin position="25"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical" evidence="3">
        <location>
            <begin position="27"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9683675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="96" mass="9610" checksum="2AF9063D84C82296" modified="2024-01-24" version="1">MKGVVIALLVALAMVHFMAEPARAITCADVNKALAPCISYLTGGGGPTSACCGGCAKDAASRNPNIKEDAAAALPTKCGVQTNIPISRSTDCSTVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z883</accession>
    <name>A0AA38Z883_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Beta-hexosaminidase</fullName>
            <ecNumber evidence="7">3.2.1.52</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_016559</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.</text>
            <dbReference type="EC" id="3.2.1.52"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the glycosyl hydrolase 20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.52" evidence="7"/>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z883"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004563">
        <property type="term" value="F:beta-N-acetylhexosaminidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030203">
        <property type="term" value="P:glycosaminoglycan metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06562">
        <property type="entry name" value="GH20_HexA_HexB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000063">
        <property type="entry name" value="Beta-hexosaminidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.379.10">
        <property type="entry name" value="Chitobiase/beta-hexosaminidase domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025705">
        <property type="entry name" value="Beta_hexosaminidase_sua/sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015883">
        <property type="entry name" value="Glyco_hydro_20_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029018">
        <property type="entry name" value="Hex-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029019">
        <property type="entry name" value="HEX_eukaryotic_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22600">
        <property type="entry name" value="BETA-HEXOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22600:SF40">
        <property type="entry name" value="BETA-HEXOSAMINIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00728">
        <property type="entry name" value="Glyco_hydro_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14845">
        <property type="entry name" value="Glycohydro_20b2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001093">
        <property type="entry name" value="B-hxosamndse_ab_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00738">
        <property type="entry name" value="GLHYDRLASE20"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55545">
        <property type="entry name" value="beta-N-acetylhexosaminidase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041429863" description="Beta-hexosaminidase" evidence="9">
        <location>
            <begin position="26"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-hexosaminidase eukaryotic type N-terminal" evidence="11">
        <location>
            <begin position="39"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 20 catalytic" evidence="10">
        <location>
            <begin position="185"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR001093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001093-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF14845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9684124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="541" mass="61554" checksum="ECADB45911046428" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z8B3</accession>
    <name>A0AA38Z8B3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PsbP C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_016589</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z8B3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019898">
        <property type="term" value="C:extrinsic component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009654">
        <property type="term" value="C:photosystem II oxygen evolving complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015979">
        <property type="term" value="P:photosynthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1000.10">
        <property type="entry name" value="Mog1/PsbP, alpha/beta/alpha sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016123">
        <property type="entry name" value="Mog1/PsbP_a/b/a-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002683">
        <property type="entry name" value="PsbP_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040946">
        <property type="entry name" value="PSII_PsbP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31407">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31407:SF4">
        <property type="entry name" value="PSBP-LIKE PROTEIN 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01789">
        <property type="entry name" value="PsbP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55724">
        <property type="entry name" value="Mog1p/PsbP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PsbP C-terminal" evidence="1">
        <location>
            <begin position="86"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9684169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="238" mass="26551" checksum="36C8BB63C2CF7520" modified="2024-01-24" version="1">MASLQNSPAIHRTLFPNSFSQLGLQKHGQFRCCRRGVSLLVRAEQVSTTNREDGPGRRQVLAVGAIASWVSLVNQTSISFAAETKKGFLTVTDKKDGYSFLYPFGWQEVVIDGQDKVFKDVIEPLESVSVNTIPTVKQDIRELGSPQEVAEALIKKVLAPPSQKTKLVEAVEHDVDGKAYYTFEFTVQAPNFTRHALSAITIGNGKFYTVTTGANERRWEKMKDKLHTVIDSFQIFNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZBQ8</accession>
    <name>A0AA38ZBQ8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">PVL29_015116</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="25">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="25">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZBQ8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00028">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.90.10.10:FF:000039">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase SD2-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.90.10.30:FF:000003">
        <property type="entry name" value="Os04g0303100 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000248">
        <property type="entry name" value="S-receptor-like kinase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000178">
        <property type="entry name" value="serine/threonine-protein kinase PBS1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051343">
        <property type="entry name" value="G-type_lectin_kinases/EP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47976">
        <property type="entry name" value="G-TYPE LECTIN S-RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD2-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47976:SF66">
        <property type="entry name" value="G-TYPE LECTIN S-RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE SD2-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0430">Lectin</keyword>
    <keyword evidence="14 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8 22" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041283591" description="non-specific serine/threonine protein kinase" evidence="22">
        <location>
            <begin position="17"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="574"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="24">
        <location>
            <begin position="167"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="634"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="662"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="KAJ9686076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="943" mass="103761" checksum="A5BBCF004BE36AFC" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZBT9</accession>
    <name>A0AA38ZBT9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF688 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_015158</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZBT9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007789">
        <property type="entry name" value="DUF688"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33671">
        <property type="entry name" value="N-METHYLTRANSFERASE, PUTATIVE (DUF688)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33671:SF2">
        <property type="entry name" value="N-METHYLTRANSFERASE, PUTATIVE (DUF688)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05097">
        <property type="entry name" value="DUF688"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="219"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="346"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="578"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="229"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="346"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="365"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="578"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="624"/>
            <end position="653"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9686133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="691" mass="76507" checksum="F541D689375BF40D" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZBV8</accession>
    <name>A0AA38ZBV8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-CH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_015080</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZBV8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16495">
        <property type="entry name" value="RING_CH-C4HC3_MARCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46158">
        <property type="entry name" value="OS02G0165000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46158:SF2">
        <property type="entry name" value="OS02G0165000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="367"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="395"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="423"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="RING-CH-type" evidence="6">
        <location>
            <begin position="244"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="68"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="458"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="18"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="35"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="458"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="471"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="486"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="500"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="515"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9686027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="525" mass="58320" checksum="BB96237020659F29" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZEF5</accession>
    <name>A0AA38ZEF5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">WIBG Mago-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_015999</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZEF5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035145">
        <property type="term" value="C:exon-exon junction complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903259">
        <property type="term" value="P:exon-exon junction complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039333">
        <property type="entry name" value="PYM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015362">
        <property type="entry name" value="WIBG_mago-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036348">
        <property type="entry name" value="WIBG_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22959:SF0">
        <property type="entry name" value="PARTNER OF Y14 AND MAGO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22959">
        <property type="entry name" value="PYM PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09282">
        <property type="entry name" value="Mago-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01273">
        <property type="entry name" value="Mago-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101931">
        <property type="entry name" value="Pym (Within the bgcn gene intron protein, WIBG), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WIBG Mago-binding" evidence="3">
        <location>
            <begin position="28"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="70"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="155"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="183"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="23"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="85"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="155"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9687335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="236" mass="26335" checksum="C10986C4EDEEE639" modified="2024-01-24" version="1">MAMAGSEGREGEEELKQLAELSKTLKEGERLLAPTRRPDGTLRKPIRIRAGYVPQDEVAIYQSKGALWRKEMASQEGPPGYDPAMDAKPKTKSVKRNERKKEKRLQAALDKGKNLEQMVDGEVKQDGVLSAEDVNHGSEFVESVASQMNELTVTENPTTVTPPSESIECLNPGGAAPDIDKRIRALKKKIRLTEAQQQKASQQEMKPEQLDKMMKLEGWRKELKLLEDKKAELAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZF65</accession>
    <name>A0AA38ZF65_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">F-box/LRR-repeat protein 15-like leucin rich repeat domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_013934</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9687948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZF65"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001486">
        <property type="entry name" value="DNA repair protein rhp7 isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000777">
        <property type="entry name" value="RNI-like superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057207">
        <property type="entry name" value="FBXL15_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318:SF101">
        <property type="entry name" value="F-BOX_LRR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318">
        <property type="entry name" value="PARTNER OF PAIRED, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25372">
        <property type="entry name" value="DUF7885"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box/LRR-repeat protein 15-like leucin rich repeat" evidence="2">
        <location>
            <begin position="731"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="270"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="430"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="22"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="287"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="432"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9687948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="905" mass="99533" checksum="CC7E5DE5896CA473" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZG41</accession>
    <name>A0AA38ZG41_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Saposin B-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_014097</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZG41"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36058">
        <property type="entry name" value="NUCLEOPHOSMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36058:SF1">
        <property type="entry name" value="NUCLEOPHOSMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041338266" description="Saposin B-type domain-containing protein" evidence="2">
        <location>
            <begin position="29"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="167"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="222"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="224"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="245"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="295"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9688204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="304" mass="34639" checksum="A7225B58132BA076" modified="2024-01-24" version="1">MMKMKMKTIIVLVFSILVISTWLQVSHCSKKPVGVARRDDIPYIKCQVCEKLAAQLYHQVEKKQAQISPKKISEFEIIEISENVCNLKKEEADWILRIDIVEQGDKLKLVEQDSEGQCNSECKTIERACQEVMGYSDTDAAEYVYKNKPQIDSLVNYLCKDLTKACSASPPPVPKDRTPGEPFVPKSPKEAEMEKIMKSMEGMPGAPGMKMYSREDMMNMQNFGDEDADEDDDDEPHFPSKLGKVLREKESAKPDWKHTVTKGIKETGQALKRHASKVSNQIRQWWRGKITSSKKNSKAHKPEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZH12</accession>
    <name>A0AA38ZH12_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_014508</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZH12"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9688891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="180" mass="20639" checksum="6071A1E6258ECC31" modified="2024-01-24" version="1">MVRTLKVSVERKTFLVRWEGEHGGIWCSITEHSRGSVYGLGFEKEEVGWLIELWTKAIELKSHLGFNKKYKGKSCVHLMEVCFNNHGRFIRISKFTTNRKPTFLVIPEGEKGRGWENLKSALSSLLVVPPLNAYEKGRQYKVERFNHNHVGPLYRTFTNVVGDKGPRRGGIVPVGRWARG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZIH0</accession>
    <name>A0AA38ZIH0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Mitochondrial outer membrane protein porin 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_014916</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic mitochondrial porin (TC 1.B.8.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9689465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZIH0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046930">
        <property type="term" value="C:pore complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015288">
        <property type="term" value="F:porin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008308">
        <property type="term" value="F:voltage-gated monoatomic anion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07306">
        <property type="entry name" value="Porin3_VDAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.10:FF:000003">
        <property type="entry name" value="Outer mitochondrial membrane protein porin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.10">
        <property type="entry name" value="Porin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023614">
        <property type="entry name" value="Porin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001925">
        <property type="entry name" value="Porin_Euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027246">
        <property type="entry name" value="Porin_Euk/Tom40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11743:SF27">
        <property type="entry name" value="MITOCHONDRIAL OUTER MEMBRANE PROTEIN PORIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11743">
        <property type="entry name" value="VOLTAGE-DEPENDENT ANION-SELECTIVE CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01459">
        <property type="entry name" value="Porin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0626">Porin</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9689465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="277" mass="29843" checksum="AC9AE15A5D3B849E" modified="2024-01-24" version="1">MGNGPAPFSDIGKRTRDLLNKDYNFDHKFTLLMQSPGGMGVTATGWKKDQIFFGDVNTLYKSGNTTVDLKLDTHSNVSTKVTAHDILPCTKVACSFKIPDHKSGKLDVQYLHPHAAIDSSIGLNPTPLLELSAAIGSKDLSLGGEVGLNTASASFTKYNAGIGFNKPDFSVAVLLMDKGETLKASYIHSVNPPYGGTTVAAELTHKFNTYENSFTIGSAHVVDPYTMVKTRLSNNGKFAMLCQREWRPKSLITVSGEYDPKANNGATKLGLALALKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZKW0</accession>
    <name>A0AA38ZKW0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_012864</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZKW0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000660">
        <property type="entry name" value="RING/U-box superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15315">
        <property type="entry name" value="RING FINGER PROTEIN 41, 151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15315:SF102">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="151"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9690432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="247" mass="29074" checksum="1DA6435C5C5AFAFE" modified="2024-01-24" version="1">MYIASMRKSFKDSLKVLEADLQHANTLASDFPREYDGACLQMRMSYSPAAHLFLFLVQWTDCHLAGALGLLRILIYKVYVDGTTTMSTHERKASIREFYAVIYPSLLQLERGLTDTEDKKQKAVCMERYRRRDDEEHKQYSDVDIEREEECGICMEMNSKIVLPNCNHAMCLKCYREWRSRSQSCPFCRDSLKRVNSGDLWVFTDSRDIIDMVTVTRENLRRLFMYIDKLPLIIPDSLFDQYDSHLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZM50</accession>
    <name>A0AA38ZM50_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Carboxypeptidase</fullName>
            <ecNumber evidence="4">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_013730</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the peptidase S10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZM50"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF281">
        <property type="entry name" value="CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="42"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="7"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9691623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="369" mass="40943" checksum="871D12254E4108AE" modified="2024-01-24" version="1">MASHHCQPFQRPPLSLQPPSSASENLPFSGTRVPMDEVRSSYPMNVFGSIISSLFGLIFAFSFFFSPSNEEENMLYPESPTGTGFSYSANKSYTNVNDEIAARDNLVFLKNWFIKFPQYKNRELFIAGESYAGHFVPQLAQLILESSVKPLMDFDTNYNSVPHFYWSHGLISDSTYKLFSSKCNYSRMNREQTSGSLSPACLAVRSQYSQEVGDSVEQFDVTLNSCLPSLYPQPQVTENVDVCIGDKVNKYLNREDVQKSLHARLVGVANRKITMIPVMGSLVKFGIRTFVYSGDQDSVIPLFGTQILVNGLAKKLRLNTTVGGLTQVYGEILSFAIVRGGSHATPATQPARALVLFTAFLKGQPPPAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZMQ1</accession>
    <name>A0AA38ZMQ1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_011151</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9691906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZMQ1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01461">
        <property type="entry name" value="vWA_interalpha_trypsin_inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46503:SF9">
        <property type="entry name" value="INTER ALPHA-TRYPSIN INHIBITOR, HEAVY CHAIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46503">
        <property type="entry name" value="INTER-ALPHA-TRYPSIN INHIBITOR HEAVY CHAIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13768">
        <property type="entry name" value="VWA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFA" evidence="1">
        <location>
            <begin position="327"/>
            <end position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9691906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="746" mass="82415" checksum="6DC87C65DD36B531" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZMR8</accession>
    <name>A0AA38ZMR8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Cupin type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_011176</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the germin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9691941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZMR8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02241">
        <property type="entry name" value="cupin_OxOx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000047">
        <property type="entry name" value="Auxin-binding protein ABP19a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006045">
        <property type="entry name" value="Cupin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001929">
        <property type="entry name" value="Germin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31238">
        <property type="entry name" value="GERMIN-LIKE PROTEIN SUBFAMILY 3 MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00190">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00835">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0052">Apoplast</keyword>
    <keyword evidence="7 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 10" id="KW-0464">Manganese</keyword>
    <keyword evidence="5 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041449265" description="Cupin type-1 domain-containing protein" evidence="13">
        <location>
            <begin position="18"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="Cupin type-1" evidence="14">
        <location>
            <begin position="52"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="23"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9691941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="198" mass="20982" checksum="F59415B02BA19244" modified="2024-01-24" version="1">MLHVLLFFYFLVSSSHAAVQDFCVADLKAPQGPAGYSCKTPAEVTVDDFVYSGLGVAGNTSNFIKASINTAFADKFPGLNGLGLSMARVDLAPGGAIPMHSHPGSVIAAFVSSDNTVYSRTLKEGDIMVFPQGLLHFEVNTGRTPALIWVSFSSSNPGLQILSYALFSSSLPSELIEKTTFLDDDEVKRLKALLGGSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZP27</accession>
    <name>A0AA38ZP27_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">Pectinesterase</fullName>
            <ecNumber evidence="4 10">3.1.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_011096</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[(1-&gt;4)-alpha-D-galacturonosyl methyl ester](n) + n H2O = [(1-&gt;4)-alpha-D-galacturonosyl](n) + n methanol + n H(+)</text>
            <dbReference type="Rhea" id="RHEA:22380"/>
            <dbReference type="Rhea" id="RHEA-COMP:14570"/>
            <dbReference type="Rhea" id="RHEA-COMP:14573"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17790"/>
            <dbReference type="ChEBI" id="CHEBI:140522"/>
            <dbReference type="ChEBI" id="CHEBI:140523"/>
            <dbReference type="EC" id="3.1.1.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the pectinesterase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the PMEI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.11" evidence="4 10"/>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9691813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZP27"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004857">
        <property type="term" value="F:enzyme inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030599">
        <property type="term" value="F:pectinesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042545">
        <property type="term" value="P:cell wall modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045490">
        <property type="term" value="P:pectin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd15798">
        <property type="entry name" value="PMEI-like_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.40:FF:000010">
        <property type="entry name" value="Pectinesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000001">
        <property type="entry name" value="Pectinesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.40">
        <property type="entry name" value="Invertase/pectin methylesterase inhibitor family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035513">
        <property type="entry name" value="Invertase/methylesterase_inhib"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033131">
        <property type="entry name" value="Pectinesterase_Asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000070">
        <property type="entry name" value="Pectinesterase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006501">
        <property type="entry name" value="Pectinesterase_inhib_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01614">
        <property type="entry name" value="PME_inhib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31707">
        <property type="entry name" value="PECTINESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01095">
        <property type="entry name" value="Pectinesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04043">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00856">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101148">
        <property type="entry name" value="Plant invertase/pectin methylesterase inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00503">
        <property type="entry name" value="PECTINESTERASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0063">Aspartyl esterase</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="29"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="Pectinesterase inhibitor" evidence="12">
        <location>
            <begin position="68"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9691813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="578" mass="63173" checksum="5C9D8A8EF049D3E0" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZP50</accession>
    <name>A0AA38ZP50_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GDSL esterase/lipase 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_011116</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9691843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZP50"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016298">
        <property type="term" value="F:lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044552">
        <property type="entry name" value="GLIP1-5/GLL25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45966:SF12">
        <property type="entry name" value="GDSL ESTERASE_LIPASE 1-LIKE ISOFORM X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45966">
        <property type="entry name" value="GDSL-LIKE LIPASE/ACYLHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041450769" description="GDSL esterase/lipase 1" evidence="3">
        <location>
            <begin position="26"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9691843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="358" mass="39431" checksum="43A6542287505F78" modified="2024-01-24" version="1">MRSLSFQFCVLIIFGSLLIPAFCHGHDSHSQKPHKHVPLFVFGDSLFDPGNNLYLNTSHKEASAYWPYGETFFKRPTSEFMELPLTTAYLQPGTHRFTHGSNFASGGAGVLADTHPGTISLPLQLSYFKNVVKQLKQKLGEVKTKKLLMRAVYLFSIGGNDYFGYSMKNQNASQSSQTQFVGMVIRNLTNALEEIYQIGGRKIAFQNVGPLGCVPTNRAKTGNGACAEEASAMAKMHNAALANVLKNLQTRLPGFKYSIFDYYNTLSDKINHPSKYGFKEGKSACCGSGAYRASNCGGQGFGGTTTKFELCSIPGDYVWFDGGHTTERANRQLAELLWNGTPNCTAPHNIKQLFGHME</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZPC5</accession>
    <name>A0AA38ZPC5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Retrovirus-related Pol polyprotein from transposon TNT 1-94-like beta-barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_011738</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZPC5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054722">
        <property type="entry name" value="PolX-like_BBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34222">
        <property type="entry name" value="GAG_PRE-INTEGRS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34222:SF95">
        <property type="entry name" value="RRNA 2'-O-METHYLTRANSFERASE FIBRILLARIN-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22936">
        <property type="entry name" value="Pol_BBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Retrovirus-related Pol polyprotein from transposon TNT 1-94-like beta-barrel" evidence="2">
        <location>
            <begin position="324"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="204"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="263"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="204"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9692791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="424" mass="46504" checksum="D34F46B1D5568389" modified="2024-01-24" version="1">MATKNSIFSSVISGSPMITSEKLVGSENYLSWSASVELWFMGQGYENHLVTQKADIPKVDRVQWRKIDAQLCSVLWQSVDPKILLHFRAYKTCFKFWNQAKGLYTNDIQRLYKVASSIVNVRQQDMDLSTYIGQIASLKEEFLTVMPLTTDVGDQRTHIDKFFMVLTLIGLCPGLETVHDQILGSSSVPSLDDVFARLLRISSTQTSPSDNTSNSSMLVSQTNSRGGRSGNRGRCQRPPCTYYNKLGHTHDRCYQLHGQPPLTAHVAQSSDPQPLPPPSSSTSQDISLTDSGYDQATKSASITSVAQTGNASACLTHTSSLGPWILDSGTSDHISGNKDLFSSITTTSVLPTVTLANGSQTVAKGIGLAHPLTSLPLTYVLYTPECSFNLISISKITRTLNCSITFSDKFVTLQDRRTRKTIGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZPQ0</accession>
    <name>A0AA38ZPQ0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Receptor-like serine/threonine-protein kinase</fullName>
            <ecNumber evidence="17">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="ORF">PVL29_011918</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="26">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="26">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="17"/>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9693003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZPQ0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048544">
        <property type="term" value="P:recognition of pollen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00028">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00053">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01098">
        <property type="entry name" value="PAN_AP_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.90.10.10:FF:000017">
        <property type="entry name" value="Putative receptor protein kinase ZmPK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000059">
        <property type="entry name" value="S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000302">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.90.10.10:FF:000015">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.10">
        <property type="entry name" value="Bulb-type lectin domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.4.10">
        <property type="entry name" value="Hepatocyte Growth Factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000858">
        <property type="entry name" value="S_locus_glycoprot_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024171">
        <property type="entry name" value="SRK-like_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47974">
        <property type="entry name" value="OS07G0415500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47974:SF3">
        <property type="entry name" value="RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00954">
        <property type="entry name" value="S_locus_glycop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000641">
        <property type="entry name" value="SRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 17 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 18" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 17 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 17" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 21" id="KW-0732">Signal</keyword>
    <keyword evidence="4 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041368022" description="Receptor-like serine/threonine-protein kinase" evidence="21">
        <location>
            <begin position="21"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="452"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="24">
        <location>
            <begin position="17"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="23">
        <location>
            <begin position="278"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="25">
        <location>
            <begin position="330"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="509"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="537"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRNR" id="PIRNR000641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EMBL" id="KAJ9693003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="793" mass="89561" checksum="784DE2D448E757EA" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZQ86</accession>
    <name>A0AA38ZQ86_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">VanZ-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_011882</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZQ86"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35462">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35462:SF2">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9692960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="111" mass="12033" checksum="DEFB6016BCE22F5A" modified="2024-01-24" version="1">MENGDDWVAVDKVYHILFCFALTLIFSALANTTRYPFLRRYSVWVASILSLAAGAAKEVADEIGFFRSAGASPKDALADLLGVLIACLALSLRKSSRRPDPMDQVPGVSLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZSM0</accession>
    <name>A0AA38ZSM0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">PVL29_009827</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="23">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the leguminous lectin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZSM0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009738">
        <property type="term" value="P:abscisic acid-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009845">
        <property type="term" value="P:seed germination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06899">
        <property type="entry name" value="lectin_legume_LecRK_Arcelin_ConA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000108">
        <property type="entry name" value="L-type lectin-domain containing receptor kinase S.4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000096">
        <property type="entry name" value="L-type lectin-domain containing receptor kinase V.9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000491">
        <property type="entry name" value="Lectin-domain containing receptor kinase VI.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050528">
        <property type="entry name" value="L-type_Lectin-RKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001220">
        <property type="entry name" value="Legume_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27007">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00139">
        <property type="entry name" value="Lectin_legB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0430">Lectin</keyword>
    <keyword evidence="15 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9 21" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041446651" description="non-specific serine/threonine protein kinase" evidence="21">
        <location>
            <begin position="26"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="298"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="351"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="381"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAJ9694032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="681" mass="75060" checksum="98B0A49C46AA92DA" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZTD3</accession>
    <name>A0AA38ZTD3_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_010412</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZTD3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000109">
        <property type="entry name" value="POT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11654">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00854">
        <property type="entry name" value="PTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="16"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="57"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="164"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="315"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="348"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="388"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9694923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="607" mass="68542" checksum="07AE7B458A8C0756" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZU72</accession>
    <name>A0AA38ZU72_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DYW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_010528</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. PCMP-H subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZU72"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031425">
        <property type="term" value="P:chloroplast RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000344">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001050">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At2g33760"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000231">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032867">
        <property type="entry name" value="DYW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046849">
        <property type="entry name" value="E2_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF452">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14432">
        <property type="entry name" value="DYW_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20430">
        <property type="entry name" value="Eplus_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="123"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="193"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="224"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="294"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="325"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="396"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="DYW" evidence="4">
        <location>
            <begin position="540"/>
            <end position="632"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9695074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="632" mass="72004" checksum="8DC7533CCC623C06" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZUQ8</accession>
    <name>A0AA38ZUQ8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_010856</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZUQ8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052426">
        <property type="entry name" value="Plant_dev_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45801">
        <property type="entry name" value="OS07G0101800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45801:SF107">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR SUPERMAN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="22"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="86"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="321"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="326"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9695582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="353" mass="37183" checksum="EA7757E6E1A873EE" modified="2024-01-24" version="1">MQTIGNAAEGAAAPRPYVNFEYICKFCFRTFKSGHALGGHQNVHRNDQNREVGKYCGVSFGFENRSQRKVVATDQKTKRGPRVQHLRVGDGSSSGLNHQGGPTAPGWHPGVGIGLVAQDSILAMARPRLNNQGPPPPIATTLSPGSIGGETGAIYYAENMPGSRLNQGPQSASQVHPGLGDENPRIGVGSGGLRSTWAMPRAGLNVQGAHTEPGLDSVIGDNFSNLAAGNGATNGFNLRIEPTGLDENVDLTLDLTPAGAKPVRAPTMEESLKLTIAPPALAPRCNHSCRPPGQAKPPYDGFLEDHFGRWKLNNHLSKQAGSATYSETTVSSGSSGVEVSSRLSDGVDLELKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZXY5</accession>
    <name>A0AA38ZXY5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 21">Peroxidase</fullName>
            <ecNumber evidence="4 21">1.11.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">PVL29_008534</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="23">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:56136"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:139520"/>
            <dbReference type="ChEBI" id="CHEBI:139521"/>
            <dbReference type="EC" id="1.11.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18 21">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="18 21">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18 21">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="18 21">Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="21">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peroxidase family. Ascorbate peroxidase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="21">Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.7" evidence="4 21"/>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZXY5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140825">
        <property type="term" value="F:lactoperoxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00693">
        <property type="entry name" value="secretory_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.420.10:FF:000008">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.520.10:FF:000001">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.420.10">
        <property type="entry name" value="Peroxidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000823">
        <property type="entry name" value="Peroxidase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019793">
        <property type="entry name" value="Peroxidases_heam-ligand_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033905">
        <property type="entry name" value="Secretory_peroxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31235">
        <property type="entry name" value="PEROXIDASE 25-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00458">
        <property type="entry name" value="PEROXIDASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00461">
        <property type="entry name" value="PLPEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00435">
        <property type="entry name" value="PEROXIDASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50873">
        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 18 21" id="KW-0106">Calcium</keyword>
    <keyword evidence="13 20" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 21" id="KW-0349">Heme</keyword>
    <keyword evidence="15 21" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="12 18 21" id="KW-0408">Iron</keyword>
    <keyword evidence="8 18 21" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 21" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6 21" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 21" id="KW-0964">Secreted</keyword>
    <keyword evidence="9 21" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041487403" description="Peroxidase" evidence="21">
        <location>
            <begin position="23"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="Plant heme peroxidase family profile" evidence="22">
        <location>
            <begin position="24"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="16">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="18">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="19">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="34"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="67"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="119"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="199"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU362060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAJ9696358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="323" mass="35311" checksum="1AC0FEC99A085D1E" modified="2024-01-24" version="1">MKANLLLLVSMVVLGVLGVCQGGSLRKRYYRRACPHAEEIVQKVTWSHVSSNPVLPAKFIRMHFHDCFVRGCDGSVLLNSTKNSTAEKDAVPNRSLAGFDVIDDIKSQLEKTCPGVVSCADIVALAARDAVSFQFKKPMWEVLTGRRDGRVSLASEVPANIPAPVFNFSSLNQSFASKGLTVHDLVVLSGAHTIGVGHCNFFSNRLYNFTGKGDADPSLNPTYAAFLRTKCRNLTDTTAVEMDPKSSRNFDSHYFVILNQNKGLFQSDAALLTNKGARRIALELRKSADFFTEFGQSMKRMGAIGVLTGKAGEIRKKCSLVNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZZR7</accession>
    <name>A0AA38ZZR7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">SOSEKI DIX-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_007427</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="8">Homodimer. Forms long polymer filaments with other SOKs proteins polymers (e.g. SOK1, SOK2, SOK3 and SOK4) crucial for polar localization and biological activity. Binds to ANGUSTIFOLIA (AN).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the SOSEKI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZZR7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051258">
        <property type="term" value="P:protein polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051302">
        <property type="term" value="P:regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000067">
        <property type="term" value="P:regulation of root morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090708">
        <property type="term" value="P:specification of plant organ axis polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010369">
        <property type="entry name" value="SOK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048351">
        <property type="entry name" value="SOK_DIX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021182">
        <property type="entry name" value="SOK_magnoliopsida"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31083:SF4">
        <property type="entry name" value="PROTEIN SOSEKI 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31083">
        <property type="entry name" value="UPSTREAM OF FLC PROTEIN (DUF966)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06136">
        <property type="entry name" value="SOK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF031043">
        <property type="entry name" value="UCP031043"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SOSEKI DIX-like" evidence="10">
        <location>
            <begin position="39"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="127"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="183"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="396"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="17"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="145"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="210"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF06136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9698343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="428" mass="47843" checksum="AA270E8B5EE08034" modified="2024-01-24" version="1">MAATTSRARAELQLPKKWKERETSPERTKVWTEPNRRKVSVVYYLSRNGQLEHPHFMEVPLSSSDGLFLRDVINRLNVLRGNGMAGMYCWSAKRSYKNGFVWHDLAENDFIYPAHGQEYVLKGSELLEGSPKPKSNETMSSSFRKPPETHKSSDDSDFPVVVRRRNQSWSSIDLHEYKVYKAESTGESAGKAAADASTQTDDKRRRRRMIREEEVEPKTEEQEPYQNHSTELSREEISPPPSDSSPETLESLMKADGRLILGAGGGNQDQTVSITNPSVGRMKASSVLMQLISCGSMSFKDCGPTIVKDQGLALISQYKARLPRGAANQETSMENPRYGGVRLEDKEYFSGSLIETKKDQFPALKRSSSYNADGSSQLEMGDKEIDGVRAKCIPRKSKTSLAKKEGNAEISCRTSQHGSKRLEGSTPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZZR9</accession>
    <name>A0AA38ZZR9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Dof zinc finger protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_007353</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Transcription factor that binds specifically to a 5'-AA[AG]G-3' consensus core sequence.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZZR9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045174">
        <property type="entry name" value="Dof"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003851">
        <property type="entry name" value="Znf_Dof"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31992">
        <property type="entry name" value="DOF ZINC FINGER PROTEIN DOF1.4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31992:SF221">
        <property type="entry name" value="DOF ZINC FINGER PROTEIN DOF3.2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02701">
        <property type="entry name" value="Zn_ribbon_Dof"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01361">
        <property type="entry name" value="ZF_DOF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50884">
        <property type="entry name" value="ZF_DOF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3 9" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 8 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Dof-type" evidence="11">
        <location>
            <begin position="38"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="83"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="106"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU369094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9698221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="296" mass="32567" checksum="EDA3F966EBF21022" modified="2024-01-24" version="1">MDASSAQRQEMDTQSLESMLVCSKAQQERKPRPPEQALKCPRCDSTNTKFCYYNNYSLSQPRYFCKSCRRYWTKGGTLRNVPVGGGCRKNKRSSSSSSSKRTQDQPLTTNPNPLNVLPPLTYDSNDLTLAFARLQKQNGGQLGFDDHHHDLALLGNPNNTHCDVLGNQCVNTCGTTTPGLLDALRSNFLETPSGYHNLYYGFGNGGMGGEVENGGVGGEHMVLPYEEMSGATTTAVTVTTMKQELCHGREGENRVLWGFPWQQLNPDGSMGEVDSGRESWNGLASSWHGLLNSPLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A0H8</accession>
    <name>A0AA39A0H8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_007662</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A0H8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050231">
        <property type="entry name" value="Iron_ascorbate_oxido_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47990">
        <property type="entry name" value="2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00682">
        <property type="entry name" value="IPNSYNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0408">Iron</keyword>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="4">
        <location>
            <begin position="148"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9698706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="293" mass="33391" checksum="0F3871FC2589966F" modified="2024-01-24" version="1">MDLECMEMEKLGEVCREWGIFRLVNHGIPLSLLSQLEDHAKRLFGLPFESKEAIFTTPVSYFWGTPALTKSGVALSQTSQNVNWVEGFNVPLSQLPLLQTQDPIIDSFRLILEEYGRHLSRIARTLFNAMAKNLGHDPKQCESSLDESSGIVRVYRYPLRPNRDHASHMEVHTDSSVLSILNQDEVGGLEVLKDGEWLSASPISNTLVINVGDMMQVISNDEYKSVIHRVKVAKERERLSICYFVFPEENTIIKSSKYRPFSYNDFRSQVQEDIKALGHKVGLERFKVNNDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A2W8</accession>
    <name>A0AA39A2W8_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_008146</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DNA glycosylase family. DEMETER subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9699417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035514">
        <property type="term" value="F:DNA demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019104">
        <property type="term" value="F:DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141166">
        <property type="term" value="P:chromosomal 5-methylcytosine DNA demethylation pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="entry name" value="Helix-hairpin-Helix base-excision DNA repair enzymes (C-terminal)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044811">
        <property type="entry name" value="DME/ROS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003651">
        <property type="entry name" value="Endonuclease3_FeS-loop_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028924">
        <property type="entry name" value="Perm-CXXC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028925">
        <property type="entry name" value="RRM_DME"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46213:SF13">
        <property type="entry name" value="DEMETER-LIKE PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46213">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR DEMETER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15629">
        <property type="entry name" value="Perm-CXXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15628">
        <property type="entry name" value="RRM_DME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00525">
        <property type="entry name" value="FES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Permuted single zf-CXXC unit" evidence="12">
        <location>
            <begin position="1225"/>
            <end position="1256"/>
        </location>
    </feature>
    <feature type="domain" description="Demeter RRM-fold" evidence="11">
        <location>
            <begin position="1259"/>
            <end position="1359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="89"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="171"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="270"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="787"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="101"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="179"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="270"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="282"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="305"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="332"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="787"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="803"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="820"/>
            <end position="831"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF15628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF15629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9699417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1374" mass="155572" checksum="2A126F9A018A74CB" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A362</accession>
    <name>A0AA39A362_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_007896</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9699044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A362"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9699044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="83" mass="9547" checksum="7A4F2A368057FE02" modified="2024-01-24" version="1">MGHVKEDVTIAMAVVGVSDGDDIPLDKKTETGEEQEFKRESERKKRGSEKNLVELEREPERIPAAGFKEFSSCLRRVTERQRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A4A2</accession>
    <name>A0AA39A4A2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">MD-2-related lipid-recognition domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_008275</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the intermembrane transfer of phosphatidylglycerol and phosphatidylinositol.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NPC2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9699599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A4A2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032366">
        <property type="term" value="P:intracellular sterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00917">
        <property type="entry name" value="PG-PI_TP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.770:FF:000002">
        <property type="entry name" value="putative phosphatidylglycerol/phosphatidylinositol transfer protein DDB_G0282179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003172">
        <property type="entry name" value="ML_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033917">
        <property type="entry name" value="ML_PG-PI_TP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039670">
        <property type="entry name" value="NPC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11306">
        <property type="entry name" value="NIEMANN PICK TYPE C2 PROTEIN NPC2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11306:SF0">
        <property type="entry name" value="PHOSPHATIDYLGLYCEROL_PHOSPHATIDYLINOSITOL TRANSFER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02221">
        <property type="entry name" value="E1_DerP2_DerF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00737">
        <property type="entry name" value="ML"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041321819" description="MD-2-related lipid-recognition domain-containing protein" evidence="7">
        <location>
            <begin position="26"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="MD-2-related lipid-recognition" evidence="8">
        <location>
            <begin position="28"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9699599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="154" mass="16591" checksum="884767D825BAB775" modified="2024-01-24" version="1">MELSQLKLTLLLFFAVCLLVPSIQAKSVSYCDKKGEYAVKVQDIQISPDPVVPGKPATFTISASAGEAISGGKVVIEVSLFGVHVHTESHNLCEETSCPISGGNFELSHSQMLPGFTPPGSYTLKMKMEDESKHQLTCITFNFNIGFGSYVADS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A4C1</accession>
    <name>A0AA39A4C1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_006117</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the AP2/ERF transcription factor family. ERF subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A4C1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009873">
        <property type="term" value="P:ethylene-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000001">
        <property type="entry name" value="Ethylene-responsive transcription factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044808">
        <property type="entry name" value="ERF_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31190">
        <property type="entry name" value="DNA-BINDING DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31190:SF488">
        <property type="entry name" value="ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR ERF096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00367">
        <property type="entry name" value="ETHRSPELEMNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="8">
        <location>
            <begin position="20"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="78"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9700652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="130" mass="14613" checksum="9732A74DC4F71860" modified="2024-01-24" version="1">MDEDPKAEMKEQKEGGGEVRYRGVRRRPWGKFAAEIRDSTRHGARVWLGTFNSAEEAARAYDRAAYAMRGSLAILNFPNEYHTPGTASSSASSSMPEGDQQGKQVIELEYLDDKLLEDLLDSEDKKNKKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A846</accession>
    <name>A0AA39A846_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA topoisomerase</fullName>
            <ecNumber evidence="3">5.6.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Omega-protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Relaxing enzyme</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Swivelase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Untwisting enzyme</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PVL29_004133</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 20 21">
            <text>ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.</text>
            <dbReference type="EC" id="5.6.2.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the type IA topoisomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.1" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A846"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003917">
        <property type="term" value="F:DNA topoisomerase type I (single strand cut, ATP-independent) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00186">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03363">
        <property type="entry name" value="TOPRIM_TopoIA_TopoI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.65.10">
        <property type="entry name" value="Bacterial Topoisomerase I, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.460.10">
        <property type="entry name" value="Topoisomerase I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.20.10">
        <property type="entry name" value="Topoisomerase I, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.290.10">
        <property type="entry name" value="Topoisomerase I, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00952">
        <property type="entry name" value="Topoisom_1_prok"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000380">
        <property type="entry name" value="Topo_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003601">
        <property type="entry name" value="Topo_IA_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023406">
        <property type="entry name" value="Topo_IA_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013497">
        <property type="entry name" value="Topo_IA_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013824">
        <property type="entry name" value="Topo_IA_cen_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013825">
        <property type="entry name" value="Topo_IA_cen_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013826">
        <property type="entry name" value="Topo_IA_cen_sub3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023405">
        <property type="entry name" value="Topo_IA_core_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003602">
        <property type="entry name" value="Topo_IA_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013498">
        <property type="entry name" value="Topo_IA_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005733">
        <property type="entry name" value="TopoI_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028612">
        <property type="entry name" value="Topoisom_1_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025589">
        <property type="entry name" value="Toprim_C_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034149">
        <property type="entry name" value="TOPRIM_TopoI"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01051">
        <property type="entry name" value="topA_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42785:SF1">
        <property type="entry name" value="DNA TOPOISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42785">
        <property type="entry name" value="DNA TOPOISOMERASE, TYPE IA, CORE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01131">
        <property type="entry name" value="Topoisom_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13368">
        <property type="entry name" value="Toprim_C_rpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01396">
        <property type="entry name" value="Zn_ribbon_Top1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00417">
        <property type="entry name" value="PRTPISMRASEI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00437">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00436">
        <property type="entry name" value="TOP1Bc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56712">
        <property type="entry name" value="Prokaryotic type I DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00396">
        <property type="entry name" value="TOPO_IA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52039">
        <property type="entry name" value="TOPO_IA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0799">Topoisomerase</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Toprim" evidence="16">
        <location>
            <begin position="380"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="domain" description="Topo IA-type catalytic" evidence="17">
        <location>
            <begin position="511"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="190"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1166"/>
            <end position="1185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="239"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="271"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="288"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="298"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="324"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS52039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ9702245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1185" mass="132286" checksum="4C7DDC8127415E81" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A8M1</accession>
    <name>A0AA39A8M1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Sigma factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_003915</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9701908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A8M1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071482">
        <property type="term" value="P:cellular response to light stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06171">
        <property type="entry name" value="Sigma70_r4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1810">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000943">
        <property type="entry name" value="RNA_pol_sigma70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007624">
        <property type="entry name" value="RNA_pol_sigma70_r3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007630">
        <property type="entry name" value="RNA_pol_sigma70_r4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050239">
        <property type="entry name" value="Sigma-70_RNA_pol_init_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30603">
        <property type="entry name" value="RNA POLYMERASE SIGMA FACTOR RPO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30603:SF13">
        <property type="entry name" value="RNA POLYMERASE SIGMA FACTOR SIGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04539">
        <property type="entry name" value="Sigma70_r3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04545">
        <property type="entry name" value="Sigma70_r4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00046">
        <property type="entry name" value="SIGMA70FCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="Sigma2 domain of RNA polymerase sigma factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="Sigma3 and sigma4 domains of RNA polymerase sigma factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0731">Sigma factor</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RNA polymerase sigma-70 region 2" evidence="7">
        <location>
            <begin position="328"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase sigma-70 region 3" evidence="6">
        <location>
            <begin position="407"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase sigma-70 region 4" evidence="8">
        <location>
            <begin position="495"/>
            <end position="547"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9701908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="561" mass="63517" checksum="F23A390C434687F4" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A948</accession>
    <name>A0AA39A948_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_004046</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A948"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318:SF101">
        <property type="entry name" value="F-BOX_LRR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318">
        <property type="entry name" value="PARTNER OF PAIRED, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9702119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="643" mass="71289" checksum="30C13C1A04158BEE" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A9M2</accession>
    <name>A0AA39A9M2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_005008</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A9M2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000595">
        <property type="entry name" value="ATPase family AAA domain-containing protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021911">
        <property type="entry name" value="ATAD3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23075:SF13">
        <property type="entry name" value="AAA-TYPE ATPASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23075">
        <property type="entry name" value="PUTATIVE ATP-ASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12037">
        <property type="entry name" value="ATAD3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041313394" description="AAA+ ATPase domain-containing protein" evidence="6">
        <location>
            <begin position="25"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="383"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="33"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="188"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="34"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="50"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9703472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="627" mass="69898" checksum="C3BC57C1A966B6B9" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AAN4</accession>
    <name>A0AA39AAN4_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_004576</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AAN4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038934">
        <property type="entry name" value="At5g64816-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35548">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35548:SF1">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9702899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="126" mass="14031" checksum="4A714FCA95361EF7" modified="2024-01-24" version="1">MVEVWWSLLGAAIPAIVAGQALRMKRRHAEEQRIKSARGREKTSDEIFVCERVCTSKRMLKKVGTFSKDPSPDTCVTVCGVSELDACSDACARTVCVNQHQVPNWNDICLRRCQSECLKLSASRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AAZ6</accession>
    <name>A0AA39AAZ6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ATP synthase subunit e, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_002712</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATPase e subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AAZ6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015078">
        <property type="term" value="F:proton transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015986">
        <property type="term" value="P:proton motive force-driven ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008386">
        <property type="entry name" value="ATP_synth_F0_esu_mt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36028:SF6">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT E, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36028">
        <property type="entry name" value="OSJNBB0050O03.8 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05680">
        <property type="entry name" value="ATP-synt_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="4" id="KW-0138">CF(0)</keyword>
    <keyword evidence="5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9704283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="53" mass="5544" checksum="752CB922C83A3165" modified="2024-01-24" version="1">MAPPPGPYSGTSTLALVARVSAFSAGLVYGSIKLKYLKAKAKSQKKAEAKSHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AB49</accession>
    <name>A0AA39AB49_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">amidophosphoribosyltransferase</fullName>
            <ecNumber evidence="4">2.4.2.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_002711</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>5-phospho-beta-D-ribosylamine + L-glutamate + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + L-glutamine + H2O</text>
            <dbReference type="Rhea" id="RHEA:14905"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58681"/>
            <dbReference type="EC" id="2.4.2.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.14" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AB49"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004044">
        <property type="term" value="F:amidophosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009113">
        <property type="term" value="P:purine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00715">
        <property type="entry name" value="GPATase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01931">
        <property type="entry name" value="PurF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005854">
        <property type="entry name" value="PurF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035584">
        <property type="entry name" value="PurF_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01134">
        <property type="entry name" value="purF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11907">
        <property type="entry name" value="AMIDOPHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13537">
        <property type="entry name" value="GATase_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="12">
        <location>
            <begin position="95"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9704282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="582" mass="63002" checksum="5B9B0D76C9F3CC71" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AC34</accession>
    <name>A0AA39AC34_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Allene oxide synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_003096</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AC34"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016125">
        <property type="term" value="P:sterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11071">
        <property type="entry name" value="CYP74"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000024">
        <property type="entry name" value="Allene oxide synthase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286:SF302">
        <property type="entry name" value="ALLENE OXIDE SYNTHASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286">
        <property type="entry name" value="CYTOCHROME P450 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0349">Heme</keyword>
    <keyword evidence="4 6" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="6">
        <location>
            <position position="431"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR602403-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9704883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="479" mass="53492" checksum="D80528F0AB7A70D0" modified="2024-01-24" version="1">MSSSSSSSSSSQKDLNSSSSLSKLPLRKIPGDYGLPFFGREEFFNARMHKYQSTVFRANMPPGPFIASNPNVIVLLDSISFPILFDTSKVEKRNVLDGTYMPSTAFTGGYRVCAYLDPSETSHAPLKRLFMSALAARHHNFIPLFRNSLSELFTSLEDDISSKGEADFNDISDNMSFNFVFRLFCDKYPSETALGSQGPSIVTKWLFFQLAPLITLGLSLLPDVVEDLLLHTFPLPSIFVKSDYKKLYRAFYASASSILDEAESMGIKRDEACHNLVFLAGFNAYGGMKALFPSLIKWVGSAGEKLHRQLADEIRAVVKAEGGVSFAALEKMSLTKSVVYEALRIEPPVPFQYGKAKEDMVIHSHDAAFEIKKGEMIFGYQPFATKDPKVFENPVEFIGNRFMGEGERLLKYVYWSNGRESDNPTVENKQCAGKDLVLLLSRVMLVEFFLRYDTFDIESGTLLLGSSVTFKSITKATYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ACW6</accession>
    <name>A0AA39ACW6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Late embryogenesis abundant protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_003317</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ACW6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004926">
        <property type="entry name" value="LEA_3a"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35109">
        <property type="entry name" value="GLUTAMATE RACEMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35109:SF1">
        <property type="entry name" value="GLUTAMATE RACEMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03242">
        <property type="entry name" value="LEA_3a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9705196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="102" mass="10957" checksum="F149968C9D953A8B" modified="2024-01-24" version="1">MARSGVAKTRMLFLREATLVWRGLSKVAGAEGAPGKARGQTDDADNKGDGSSSSWVPHPRTGIYFPKGHDHVMDDVPDGAACFGQTYWLRTVDGVDKPDPDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AFU5</accession>
    <name>A0AA39AFU5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">CSC1-like protein At1g69450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_003613</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CSC1 (TC 1.A.17) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AFU5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005227">
        <property type="term" value="F:calcium-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045122">
        <property type="entry name" value="Csc1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003864">
        <property type="entry name" value="CSC1/OSCA1-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027815">
        <property type="entry name" value="CSC1/OSCA1-like_cyt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032880">
        <property type="entry name" value="CSC1/OSCA1-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018:SF104">
        <property type="entry name" value="ERD (EARLY-RESPONSIVE TO DEHYDRATION STRESS) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018">
        <property type="entry name" value="PROBABLE MEMBRANE PROTEIN DUF221-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14703">
        <property type="entry name" value="PHM7_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02714">
        <property type="entry name" value="RSN1_7TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13967">
        <property type="entry name" value="RSN1_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="86"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="307"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="400"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="434"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="498"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="551"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="578"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like N-terminal transmembrane" evidence="13">
        <location>
            <begin position="6"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like cytosolic" evidence="14">
        <location>
            <begin position="137"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like 7TM region" evidence="12">
        <location>
            <begin position="306"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="181"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF13967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF14703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9705628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="663" mass="76052" checksum="222C3FEF57D0BE54" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AHG7</accession>
    <name>A0AA39AHG7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_002219</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AHG7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF78">
        <property type="entry name" value="OS12G0587133 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="34"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9707127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="766" mass="87351" checksum="07B446AB243131AB" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AHT8</accession>
    <name>A0AA39AHT8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein FAR1-RELATED SEQUENCE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_000127</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Putative transcription activator involved in regulating light control of development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the FHY3/FAR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9707901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AHT8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004330">
        <property type="entry name" value="FAR1_DNA_bnd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031052">
        <property type="entry name" value="FHY3/FAR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018289">
        <property type="entry name" value="MULE_transposase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006564">
        <property type="entry name" value="Znf_PMZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31669:SF62">
        <property type="entry name" value="PROTEIN FAR1-RELATED SEQUENCE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31669">
        <property type="entry name" value="PROTEIN FAR1-RELATED SEQUENCE 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03101">
        <property type="entry name" value="FAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10551">
        <property type="entry name" value="MULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04434">
        <property type="entry name" value="SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00575">
        <property type="entry name" value="ZnF_PMZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="8">
        <location>
            <begin position="603"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="135"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="739"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="141"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="747"/>
            <end position="758"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9707901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="893" mass="102993" checksum="D24784BF4A88C470" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D3E1</accession>
    <name>A0AA39D3E1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Ribonuclease III domain-containing protein RNC1, chloroplastic</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Chloroplast ribonuclease III domain protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_026822</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Binds specific group II introns in chloroplasts and facilitates their splicing. Acts on both subgroup IIA and subgroup IIB introns. The substrates of the subgroup II also require the CRM domain proteins CAF1 or CAF2. Binds both single-stranded and double-stranded RNA non-specifically, but lacks endonuclease activity. Required for plastid ribosome biogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Interacts with RNA. Part of large ribonucleo-protein particles that contain CAF1 and/or CAF2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D3E1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00593">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1520.10:FF:000009">
        <property type="entry name" value="Ribonuclease III domain-containing protein RNC1, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1520.10:FF:000011">
        <property type="entry name" value="Ribonuclease III domain-containing protein RNC1, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1520.10">
        <property type="entry name" value="Ribonuclease III domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000999">
        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036389">
        <property type="entry name" value="RNase_III_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11207">
        <property type="entry name" value="RIBONUCLEASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11207:SF34">
        <property type="entry name" value="RIBONUCLEASE III DOMAIN-CONTAINING PROTEIN RNC1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00636">
        <property type="entry name" value="Ribonuclease_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00535">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69065">
        <property type="entry name" value="RNase III domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50142">
        <property type="entry name" value="RNASE_3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="3" id="KW-0934">Plastid</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="RNase III" evidence="14">
        <location>
            <begin position="172"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00057170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00065223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00071225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00075737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9670523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="542" mass="63533" checksum="689812C4A4814644" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D4W2</accession>
    <name>A0AA39D4W2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_027004</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D4W2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12432">
        <property type="entry name" value="RRM_ACINU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.30">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034257">
        <property type="entry name" value="Acinus_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032552">
        <property type="entry name" value="RSB_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003034">
        <property type="entry name" value="SAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036361">
        <property type="entry name" value="SAP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47031">
        <property type="entry name" value="SAP DNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47031:SF3">
        <property type="entry name" value="SAP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16294">
        <property type="entry name" value="RSB_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02037">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00513">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68906">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50800">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAP" evidence="2">
        <location>
            <begin position="13"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="154"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="253"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="409"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="433"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="551"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="159"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="264"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="325"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="365"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="415"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="433"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="552"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="562"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="585"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="608"/>
            <end position="618"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9670809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="693" mass="76543" checksum="DD7FDFC088C04C68" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D6Q8</accession>
    <name>A0AA39D6Q8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_027307</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9671257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D6Q8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF78">
        <property type="entry name" value="OS12G0587133 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase zinc-binding" evidence="1">
        <location>
            <begin position="286"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9671257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="478" mass="55330" checksum="C5F145F72AA600A3" modified="2024-01-24" version="1">MLNCKASGWPILYLGLPLGGNPKACGFWDPVIERISRRLDGWQKAYLSFGGRITLIQSCLTHMPCYFLSLFKIPASVAAKIERMQRDFLWSGVGEGKRDHLVNWDVVCKPKSRGGLGFGKISIRNVALLGKWLWRYPREGSALWHQVILSIYGSHSNGWDANNIVRWSHRCPWKAIALVYQEFSKFTRFVVGNGDRIRFWDDLWWGEQPLGVQYPRLLSVVTDKNALISSILGYTRPFSWNFNFRRNLSDFEIEDLEGLMRSFDRLRISPSVPDKRSWSLSPSGLFTVKSFFLALSQYSELPLVFPTKIVWNAQVPFKVKSFVWLVAHKKVNTNDLLQLRRPYKALSPDICKLCMKHGETVDHLFLHYSLTMGLWHRLFQSAKMDWVSPRSISDMLSSNFNGFGSSKRGIVLWQNACIALMWVVWRERNARIFEDKARNAEYLWDSICFLTSFWAFCSKVFKGIPLNILQLDWLAACN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D9K1</accession>
    <name>A0AA39D9K1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_023635</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D9K1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47938:SF2">
        <property type="entry name" value="OS06G0184866 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47938">
        <property type="entry name" value="RESPIRATORY COMPLEX I CHAPERONE (CIA84), PUTATIVE (AFU_ORTHOLOGUE AFUA_2G06020)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12854">
        <property type="entry name" value="PPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="155"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="190"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="225"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="260"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9674197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="312" mass="35701" checksum="F43BC5332F740C4E" modified="2024-01-24" version="1">MLKPGFSPSDVDEALRAQSDPDLALDIFRWTGQQRGYRHNHVTYLTMIKIAVSGKRFRHAETLIEEVIAGACSASVPLYNSIIRFCCGRKFLFNRAFDVYKKMLNSEDCKPSLETYTLLLNALLRKFNKLNVCYVYLHAVRSLTKQMKVSGVIPDTFALNMIIKAYSKCLEIDEAIRVFREMGLYGCEPNAYTYGYIANGLCEKGRVSQGLGFYREMRSKDLVPSSSTYMILICSLSMERRFEEATKILFDMLGNSMAPDLLTYKTLLEGLCREGKCNDAFELLEELRKKDAWMGEKTYKTLVKGLHFLSQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D9M2</accession>
    <name>A0AA39D9M2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PVL29_022112</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9676958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D9M2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000590">
        <property type="entry name" value="PR5-like receptor kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000178">
        <property type="entry name" value="serine/threonine-protein kinase PBS1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045874">
        <property type="entry name" value="LRK10/LRL21-25-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032872">
        <property type="entry name" value="WAK_assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025287">
        <property type="entry name" value="WAK_GUB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27009">
        <property type="entry name" value="RUST RESISTANCE KINASE LR10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13947">
        <property type="entry name" value="GUB_WAK_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14380">
        <property type="entry name" value="WAK_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 18" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041367200" description="non-specific serine/threonine protein kinase" evidence="18">
        <location>
            <begin position="29"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="269"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="338"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="621"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="636"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="366"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAJ9676958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="646" mass="72943" checksum="7AD6068D9FF9657A" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D9T2</accession>
    <name>A0AA39D9T2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Tetraspanin-8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_022237</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D9T2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044991">
        <property type="entry name" value="TET_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32191">
        <property type="entry name" value="TETRASPANIN-8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="26"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="63"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="91"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="252"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9677138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="322" mass="36322" checksum="0A9CB2EA4292CE4B" modified="2024-01-24" version="1">MFSLHKFLPSHFEGFTPNFPRKFHNVLMAILNVLTMLIALASLAAGIYFHFHGGTHCQKFLQTPLLLVGAFLFVVSLCGLVGSTFKVSFLLWIYLFVVFLMILGLLCFTIFALVVTNKGVSQVVSNRGYKEYRLGDYSNWLQNHLVNDKNWGRIKSCLMDTDICSRLGKEINDDAAAFYKKNLSPIQSGCCKPPTDCGYEFKNATFWVVPKTGPAVADTDCKTWSNDQKQLCYDCKSCRAGLLANIKSQWRTLAICNACIFVVLIFIYSVGCCAFRNNRRDKYYKYQRSSYTQWGAAPSGITEQTNANTREASHKPTGHTFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DAJ2</accession>
    <name>A0AA39DAJ2_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_022737</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DAJ2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33240:SF15">
        <property type="entry name" value="GAG-PRO-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33240">
        <property type="entry name" value="OS08G0508500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9677933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="515" mass="57127" checksum="6CD1D1E412B13A14" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DAU3</accession>
    <name>A0AA39DAU3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ARC6 IMS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_022954</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9678218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DAU3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009706">
        <property type="term" value="C:chloroplast inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010020">
        <property type="term" value="P:chloroplast fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058032">
        <property type="entry name" value="CDP1-like_a_solenoid_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057137">
        <property type="entry name" value="CDP1-like_a_solenoid_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025344">
        <property type="entry name" value="CDP1-like_IMS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044685">
        <property type="entry name" value="CPD1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33925:SF2">
        <property type="entry name" value="PLASTID DIVISION PROTEIN CDP1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33925">
        <property type="entry name" value="PLASTID DIVISION PROTEIN CDP1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23468">
        <property type="entry name" value="ARC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13355">
        <property type="entry name" value="ARC6-like_IMS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25515">
        <property type="entry name" value="Arm_PDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Plastid division protein CDP1-like 1st alpha solenoid" evidence="4">
        <location>
            <begin position="165"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="Plastid division protein CDP1-like 2nd alpha solenoid" evidence="3">
        <location>
            <begin position="356"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="Plastid division protein CDP1-like IMS" evidence="2">
        <location>
            <begin position="693"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="426"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9678218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="819" mass="90019" checksum="1EEE02FF75C92D12" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DBP7</accession>
    <name>A0AA39DBP7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycosyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_022568</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="5"/>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DBP7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080043">
        <property type="term" value="F:quercetin 3-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080044">
        <property type="term" value="F:quercetin 7-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032787">
        <property type="term" value="P:monocarboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000019">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000057">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058980">
        <property type="entry name" value="Glyco_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926">
        <property type="entry name" value="GLUCOSYL/GLUCURONOSYL TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926:SF1526">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26168">
        <property type="entry name" value="Glyco_transf_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase N-terminal" evidence="6">
        <location>
            <begin position="8"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF26168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9677650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="457" mass="50578" checksum="6AC3F6523BF3632D" modified="2024-01-24" version="1">MEKQGGHVVVLTYPAQGHINPLLQFSKRLASKGLKVTLATTHYTVNFIQSDRVGVEAISDGFDEGGFKQAPSLEAYLESFQTVGSRTLGELILKFNESASPVDCLVYDSILPWGLSVARQFGIYGAAFWTNSASVCSMYWQLRQGLLSLPVKQETVPVSMPGLPPLGLSDLPDFLAQPGQHSAYLSAVMEQISSLEQNDWVFMNSFDALESELVKAMSGLWSVVMIGPMVPSAYLDQQIEGDTVYGASLWKPTNDECLGWLETKPPKSVVYISFGSMAEIPVKQVEEIAWGLKESDYHFIWVVKESESGKLPIDFLNSMNETGLVVTWCNQLEVLAHKAVGCFVTHCGWNSILEGLSLGVPMVGMPQRVDQPTNAKFVEDVWREGVRAQKDEEGIVTQKELEKCIREIMVGERSEEIKRNACMWRESAKSAVSKNGSSDKNTDEFVGVLLKRGKKKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DGB9</accession>
    <name>A0AA39DGB9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Trichome birefringence-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_018806</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PC-esterase family. TBL subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DGB9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016413">
        <property type="term" value="F:O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029962">
        <property type="entry name" value="TBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026057">
        <property type="entry name" value="TBL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025846">
        <property type="entry name" value="TBL_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285">
        <property type="entry name" value="PROTEIN TRICHOME BIREFRINGENCE-LIKE 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285:SF13">
        <property type="entry name" value="TRICHOME BIREFRINGENCE-LIKE N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13839">
        <property type="entry name" value="PC-Esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14416">
        <property type="entry name" value="PMR5N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Trichome birefringence-like N-terminal" evidence="8">
        <location>
            <begin position="77"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Trichome birefringence-like C-terminal" evidence="7">
        <location>
            <begin position="131"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF14416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9682961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="439" mass="50539" checksum="54A772F41C23BAA2" modified="2024-01-24" version="1">MLGFLLHIYTFFPMRVPAIEPPNGKNTPHSTPQKLVHLSLTLILLTLIPLCLMNRSPTPVQSPKIHTGGSASLAIKKKCDMFSGRWVLYPNGPSYTNATCREIIPQQNCAKFGRPDTEFLKWRWKPDGCELPRFDPVQFLELVRGKSIGFIGDSVGRNQMQSLLCLLSNVTYPVDISYKYTSNTNFKRWFYTDYKFTLATFWSPFLVKAKDADPKGHSLNNLMNLYLDTVDEAWAAQIENFDYVIISAGQWFFRPLIFYENGQVVGCHGCNEKNITEVTKFYGYRKAFQTSFRTLQNLKNYKGLTFLRTFSPAHFENGAWNEGGNCARTRPFTKQEMKLDDYNLEFYLTQVEELRAAEREGRKRGLEFRLLDVTEAMVLRPDGHPNYYGLSPHKNVTIADCVHWCTPGPIDTWNEFLLQMLKMEAERRSFDGTLQKKAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DI22</accession>
    <name>A0AA39DI22_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_017123</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DI22"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9684971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="106" mass="11677" checksum="E46731CEA1245D77" modified="2024-01-24" version="1">MAINDKNGENTDLESRLGFSLSEPPISRHPNQRNVTRSSMDKGKSKMVGPGKLLVKKTRRIIQSIIAEAPSPTPVTKSAPSPSRPLQFQFTKAPKKKLATSARECY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DIU2</accession>
    <name>A0AA39DIU2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Phosphofructokinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_019309</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>beta-D-fructose 6-phosphate + ATP = beta-D-fructose 1,6-bisphosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DIU2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003872">
        <property type="term" value="F:6-phosphofructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.450:FF:000002">
        <property type="entry name" value="ATP-dependent 6-phosphofructokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022953">
        <property type="entry name" value="ATP_PFK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050929">
        <property type="entry name" value="PFKA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000023">
        <property type="entry name" value="Phosphofructokinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035966">
        <property type="entry name" value="PKF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45770">
        <property type="entry name" value="ATP-DEPENDENT 6-PHOSPHOFRUCTOKINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00365">
        <property type="entry name" value="PFK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00476">
        <property type="entry name" value="PHFRCTKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53784">
        <property type="entry name" value="Phosphofructokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphofructokinase" evidence="12">
        <location>
            <begin position="176"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9683677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="562" mass="62526" checksum="048D4F12F43047C5" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DJ22</accession>
    <name>A0AA39DJ22_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="6">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PVL29_017452</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It probably triggers the ubiquitin-mediated degradation of different substrates. Mediates the proteasomal-dependent degradation of ATG6, a component of the autophagosome complex. Requires TRAF1A/MUSE14 and TRAF1B/MUSE13 to target ATG6 for ubiquitination and subsequent regulation of autophagosome assembly. Modulates directly the ubiquitination and proteasomal-dependent degradation of FREE1, a component of the ESCRT-I complex. Modulates directly the ubiquitination and proteasomal-dependent degradation of ELC/VPS23A, a component of the ESCRT-I complex.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Autophagosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endosome</location>
            <location evidence="3">Multivesicular body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SINA (Seven in absentia) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="6"/>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DJ22"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005771">
        <property type="term" value="C:multivesicular body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000785">
        <property type="term" value="P:regulation of autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16571">
        <property type="entry name" value="RING-HC_SIAHs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03829">
        <property type="entry name" value="Sina"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000041">
        <property type="entry name" value="E3 ubiquitin-protein ligase SINAT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.210.10:FF:000004">
        <property type="entry name" value="E3 ubiquitin-protein ligase SINAT5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000311">
        <property type="entry name" value="E3 ubiquitin-protein ligase SINAT5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.210.10">
        <property type="entry name" value="Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018121">
        <property type="entry name" value="7-in-absentia-prot_TRAF-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052088">
        <property type="entry name" value="E3_ubiquitin-ligase_SINA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049548">
        <property type="entry name" value="Sina-like_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008974">
        <property type="entry name" value="TRAF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013010">
        <property type="entry name" value="Znf_SIAH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10315">
        <property type="entry name" value="E3 UBIQUITIN PROTEIN LIGASE SIAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10315:SF111">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE DIS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21362">
        <property type="entry name" value="Sina_RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03145">
        <property type="entry name" value="Sina_TRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21361">
        <property type="entry name" value="Sina_ZnF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51081">
        <property type="entry name" value="ZF_SIAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0967">Endosome</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="90"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="SIAH-type" evidence="17">
        <location>
            <begin position="143"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="10"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00054480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ9685420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="338" mass="38380" checksum="7B159891851189E2" modified="2024-01-24" version="1">MGLSNWLASSKRKKTRSKGGKCDLESKQQAILVMASGNTYFDDMRSKSEVIDPPQSEDMMEVSEHVNDPAQTTLKPNVTVSSSVRELLECPVCLNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQSFGCLGIYPYYSKLKHESQCVYRPYNCPYAGSECTVIGDIPYLVAHLKDDHKVDMHNGSTFNHRYVKSNPHEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYSYSLEVGGTGRKMIWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGRIWKEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DJR0</accession>
    <name>A0AA39DJR0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Nucleobase-ascorbate transporter 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_015430</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DJR0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006043">
        <property type="entry name" value="NCS2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037981">
        <property type="entry name" value="NCS2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11119">
        <property type="entry name" value="XANTHINE-URACIL / VITAMIN C PERMEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00860">
        <property type="entry name" value="Xan_ur_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="64"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="88"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="125"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="152"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="177"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="213"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="356"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="384"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="415"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="453"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9686529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="520" mass="57093" checksum="5714C05EAB61F763" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DKC5</accession>
    <name>A0AA39DKC5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein RAE1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_015840</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DKC5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000146">
        <property type="entry name" value="protein RAE1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10971">
        <property type="entry name" value="MRNA EXPORT FACTOR AND BUB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="22"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="69"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="111"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="250"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9687144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="350" mass="38893" checksum="9EB39211047A6B27" modified="2024-01-24" version="1">MSNFSSITTASNPNPNKSIEVAQPPSDSVSSLSFSPKANFLVATSWDNQVRCWEIMRNGPTVGSVPKASISHDQPVLCSAWKDDGTTVFSGGCDKQVKMWPLLSGGQPMTVAMHDAPIKEVAWIPEMNLLATGSWDKTLKYWDTRQPNPVHTQQLPDRCYALTVRHPLMVVGTADRNLIVFNLQNPQTEFKRITSPLKYQTRCVAAFPDQQGFLVGSIEGRVGVHHLDDAQQSKNFTFKCHREGNEIYSVNSLNFHPVHHTFATAGSDGAFNFWDKDSKQRLKAMSRCSQPIPCSTFNNDGSIFAYSVCYDWSKGAENHNPATAKTYIFLHLPQEAEVKGKPRIGSSGRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DKX8</accession>
    <name>A0AA39DKX8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Beta-glucosidase 12-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_017759</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the glycosyl hydrolase 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DKX8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019762">
        <property type="term" value="P:glucosinolate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009651">
        <property type="term" value="P:response to salt stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000020">
        <property type="entry name" value="Beta-glucosidase 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033132">
        <property type="entry name" value="GH_1_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001360">
        <property type="entry name" value="Glyco_hydro_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018120">
        <property type="entry name" value="Glyco_hydro_1_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353:SF318">
        <property type="entry name" value="BETA-GLUCOSIDASE 31-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353">
        <property type="entry name" value="GLYCOSYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00232">
        <property type="entry name" value="Glyco_hydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00131">
        <property type="entry name" value="GLHYDRLASE1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00572">
        <property type="entry name" value="GLYCOSYL_HYDROL_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00653">
        <property type="entry name" value="GLYCOSYL_HYDROL_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041426700" description="Beta-glucosidase 12-like" evidence="7">
        <location>
            <begin position="28"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9685822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="505" mass="57073" checksum="1813E47D9BD96B8D" modified="2024-01-24" version="1">MAAHQCSLLLGLLILVSSLAWTEPVEAASFNRSSFPAGFIFGTASASYQYEGAAKEGGRGPSIWDTFSHKYPERITDGSNGDVANDFYHHYKEDVHTMKELGLDAFRFSISWSRVLPRGKLSGGVNKEGISFYNNLINELLSKGLQPYVTIFHWDLPQALEDKYGGFLSPHIIDDFRDFAELCFKEFGDRVKYWITLNEPWTYSNGGYDQGTLAPGRCSKWVNGACTAGNSAIEPYLVGHHLLLSHAAAVKVYKDKYQASQKGKIGITLVSHWMVPYSDQKVDKKAAIRALDFMFGWFMNPLTYGDYPYSMRTLVGPRLPKFTPKQSILVKGSFDFLGLNYYTANYAANVPAANTVNVSYATDSLANLTTQRNGIPIGPTTGSSWLSVYPSGIRSLLLYVKRKYNNPLIYITENGISEVNNNTLTLKEALKDPQRIDYYYRHLLFLRLAIKDGVNVKGYFAWSLLDNYEWNSGYTVRFGIVFVDYDNDLKRYPKHSAIWFKKFLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DMX6</accession>
    <name>A0AA39DMX6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_013015</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DMX6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.395.10">
        <property type="entry name" value="Adenoviral Proteinase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9690638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="109" mass="12877" checksum="96BD21BFAAA52001" modified="2024-01-24" version="1">MRISCVRTVVEECTHFFNLNGHEKNLSSFPIKRPTWVHVQDNGWDCGVHVINHMRRFDFMNSSSTPSRWDSTLIRHKLAIELLLDDENSEKAIILDNVHNHTKIKRRRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DQD4</accession>
    <name>A0AA39DQD4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">(S)-2-hydroxy-acid oxidase</fullName>
            <ecNumber evidence="3">1.1.3.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_011139</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>2-hydroxyhexanoate + O2 = 2-oxohexanoate + H2O2</text>
            <dbReference type="Rhea" id="RHEA:69372"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:35177"/>
            <dbReference type="ChEBI" id="CHEBI:133738"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:69373"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>2-hydroxyoctanoate + O2 = 2-oxooctanoate + H2O2</text>
            <dbReference type="Rhea" id="RHEA:67940"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:133514"/>
            <dbReference type="ChEBI" id="CHEBI:176689"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:67941"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a (2S)-2-hydroxycarboxylate + O2 = a 2-oxocarboxylate + H2O2</text>
            <dbReference type="Rhea" id="RHEA:16789"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:58123"/>
            <dbReference type="EC" id="1.1.3.15"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:16790"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.3.15" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9691885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DQD4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003973">
        <property type="term" value="F:(S)-2-hydroxy-acid oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050665">
        <property type="term" value="P:hydrogen peroxide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02809">
        <property type="entry name" value="alpha_hydroxyacid_oxid_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000204">
        <property type="entry name" value="Peroxisomal (S)-2-hydroxy-acid oxidase GLO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012133">
        <property type="entry name" value="Alpha-hydoxy_acid_DH_FMN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000262">
        <property type="entry name" value="FMN-dep_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037396">
        <property type="entry name" value="FMN_HAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008259">
        <property type="entry name" value="FMN_hydac_DH_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578:SF67">
        <property type="entry name" value="PEROXISOMAL (S)-2-HYDROXYACID OXIDASE GLO3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578">
        <property type="entry name" value="S -2-HYDROXY-ACID OXIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01070">
        <property type="entry name" value="FMN_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000138">
        <property type="entry name" value="Al-hdrx_acd_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00557">
        <property type="entry name" value="FMN_HYDROXY_ACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51349">
        <property type="entry name" value="FMN_HYDROXY_ACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 13" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5 13" id="KW-0288">FMN</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN hydroxy acid dehydrogenase" evidence="14">
        <location>
            <begin position="1"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="253"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="79"/>
            <end position="81"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="284"/>
            <end position="288"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="307"/>
            <end position="308"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00051933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000138-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000138-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9691885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="364" mass="39827" checksum="D645F41539816970" modified="2024-01-24" version="1">MAAEPVNVNEFQELAKQALPKMYYDYFSGGAEDQHTLRENVEAFSRITFQPRILVDVSKIDMSTTVLGFNISSPIMIAPTAQQKLAHPEGEIATARAAAACNTIMVLSFMSTCTVEEVASSCNAIRFLQLYVFKRRDVSAQLVQRAERNGFKAIVLTADTPRLGRREADIKNRMVSPQLKNFEGLISTEVVTDKGSNIEALASGIFDASLSWKDIEWLRSITNLPILIKGVLTCEDAIKAVEVGVSGIVVSNHGARQLDYVPATISALEEVVLAVGGKVPVLFDGGIRRGTDIFKALALGAQAVFIGRPVIYGLAAKGEHGVRRVIEMLKDELEITMALSGCSSVKDISRRHVRTEQDRLRSML</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DQS6</accession>
    <name>A0AA39DQS6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein FAR1-RELATED SEQUENCE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_011689</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Putative transcription activator involved in regulating light control of development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the FHY3/FAR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DQS6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004330">
        <property type="entry name" value="FAR1_DNA_bnd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031052">
        <property type="entry name" value="FHY3/FAR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018289">
        <property type="entry name" value="MULE_transposase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006564">
        <property type="entry name" value="Znf_PMZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31669:SF236">
        <property type="entry name" value="PROTEIN FAR1-RELATED SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31669">
        <property type="entry name" value="PROTEIN FAR1-RELATED SEQUENCE 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03101">
        <property type="entry name" value="FAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10551">
        <property type="entry name" value="MULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04434">
        <property type="entry name" value="SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00575">
        <property type="entry name" value="ZnF_PMZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="7">
        <location>
            <begin position="548"/>
            <end position="584"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9692724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="666" mass="77351" checksum="5FBD7A27EC571A2D" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DR31</accession>
    <name>A0AA39DR31_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glutathione S-transferase</fullName>
            <ecNumber evidence="3">2.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_011938</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Is involved in the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 3">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GST superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9693024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DR31"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03058">
        <property type="entry name" value="GST_N_Tau"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000014">
        <property type="entry name" value="Tau class glutathione S-transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045073">
        <property type="entry name" value="Omega/Tau-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11260:SF773">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE U26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11260">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE, GST, SUPERFAMILY, GST DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01152">
        <property type="entry name" value="Main.3:_Omega-_and_Tau-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="4">
        <location>
            <begin position="3"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU369102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9693024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="136" mass="16243" checksum="B61F57A9733002A6" modified="2024-01-24" version="1">MAYEIILLDFWPSMFGMKVRIALAEKGLKYEHRDEDLFNKGPLLLEMNPIHKKIPVLIHNGKPICESQIIVQYIDEVWKDKSPLLPSDPYQRAQARFWADYIDKKKSIMGRNLERMENVTKENKIKNRYVLFYEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DRT2</accession>
    <name>A0AA39DRT2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Transmembrane 9 superfamily member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_009650</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the nonaspanin (TM9SF) (TC 9.A.2) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9693789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DRT2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072657">
        <property type="term" value="P:protein localization to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004240">
        <property type="entry name" value="EMP70"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766:SF163">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY MEMBER 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02990">
        <property type="entry name" value="EMP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9693789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="110" mass="12207" checksum="DC272056E4C40C13" modified="2024-01-24" version="1">MNEELSGWKLVVGHVFREPVFLKLLCVMIGDEVQITGLAHVTFVSAAPGFMSPASRGMLLTGMIILYLFSGNFSWYVGVRLWRTVKGTSEGWSKSTSAIPISLYFIFLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DTR8</accession>
    <name>A0AA39DTR8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_009689</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9693847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DTR8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47447">
        <property type="entry name" value="OS03G0856100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47447:SF28">
        <property type="entry name" value="PENTACOTRIPEPTIDE-REPEAT REGION OF PRORP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12854">
        <property type="entry name" value="PPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="14"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="207"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="242"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="277"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="347"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="416"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="451"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="486"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="734"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="769"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="840"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="875"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="910"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="945"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="980"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="41"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9693847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1051" mass="119421" checksum="2DD3851AEF3F76EB" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DV14</accession>
    <name>A0AA39DV14_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_007200</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Heterogeneous nuclear ribonucleoprotein (hnRNP)-protein binding the poly(A) tail of mRNA and probably involved in some steps of pre-mRNA maturation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with the poly(A) tail of mRNA in nucleus.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic granule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the polyadenylate-binding RBP47 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9697973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DV14"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12344">
        <property type="entry name" value="RRM1_SECp43_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12345">
        <property type="entry name" value="RRM2_SECp43_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000405">
        <property type="entry name" value="polyadenylate-binding protein RBP45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000650">
        <property type="entry name" value="Polyadenylate-binding protein RBP45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000144">
        <property type="entry name" value="Polyadenylate-binding protein RBP47B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050825">
        <property type="entry name" value="RBM42_RBP45_47-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47640:SF10">
        <property type="entry name" value="TRNA SELENOCYSTEINE 1-ASSOCIATED PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47640">
        <property type="entry name" value="TRNA SELENOCYSTEINE 1-ASSOCIATED PROTEIN 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 10" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="41"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="134"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="253"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="15"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00057395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00063471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9697973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="447" mass="49230" checksum="D06531C5C736D851" modified="2024-01-24" version="1">MEAQAHQQQQWMHPSHASASTVSSMASSQPYTQPTSVEEVRTLWIGDLQYWVDENYLHSCFAHTGEVISIKIIRNKITGQPEGYGFVEFVSHAAAERILQAYNGTQMPGTEQTFRLNWASFGIGERRPDAGPEHSIFVGDLAPDVTDYLLQETFRAQYPSVRGAKVVTDPNTGRTKGYGFVKFSDEMERNRAMTEMNGVYCSTRPMRISAATPKKTTGFQQQYAAAAKALYPAPAYTTPPLQALPTDNDINNTTIFVGNLDPNVTEEELKQIFSQFGELVYVKIPAGRGCGFVQFGTRTSAEEAIQRMQGTVIGQLVVRISWGRSPTAKQDLPGSWGQQADPSQWSSAYYGYGQGYDAYPYGATQDPSLYAYGAYAGYLQYPQQAEGVQDMAAMAGAVPAVEQRDELYDPLATPDVDKLNAAYLAVHGSAILGRSLWLKTSPFSQMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DVF1</accession>
    <name>A0AA39DVF1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PGG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_009411</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DVF1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026961">
        <property type="entry name" value="PGG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24177">
        <property type="entry name" value="CASKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24177:SF103">
        <property type="entry name" value="PGG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13962">
        <property type="entry name" value="PGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="150"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="184"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="229"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="261"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="PGG" evidence="2">
        <location>
            <begin position="141"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9697569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="305" mass="34011" checksum="5A04CC45B8081F8E" modified="2024-01-24" version="1">MQSTDNIAHTVCTDPACTALAKGTFEWTPTGGNIVLKAVENRQSHIYDFLLNSSLLDREVLFHAVDRKGNNALHLAGKLAGDRHLQRIPTSMLQMQWEVKWYQYVQNSLPPHFVVQKNRDGSTPDEIFQMEHQKLEDGSKQWLNSTSNSCSFIAALIATVAFASSASVPGGVKQDTGEPVFGNHLAFSIFAMASLVALCCSVISLLIFLGIFISKDQDKDFTTSLPRNFLFGLTSLFISMAAMLTCFCSGNFLMLKGQLKYAAILVYALTGLLMAYFVLKHFPLFIDLLKATFRKVPERIYKEYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DVT0</accession>
    <name>A0AA39DVT0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_011068</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DVT0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16180">
        <property type="entry name" value="EFh_PEF_Group_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044590">
        <property type="entry name" value="CML48/49/50"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46824">
        <property type="entry name" value="CALCIUM-BINDING PROTEIN CML48-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46824:SF1">
        <property type="entry name" value="CALCIUM-BINDING PROTEIN CML49-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13202">
        <property type="entry name" value="EF-hand_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="113"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="179"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="46"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9695917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="284" mass="30509" checksum="BDEA3F2BDB6BA733" modified="2024-01-24" version="1">MSGYPHAPPGHGYGYGAPPPAQPYAATPYGAPPPQGHQPYAPVATPYGAPSAPYAAPGEKPPKENKPHGGSSHQPPPSSMGGYPTSSMGGYPPSAPAFGSPFASLVPSAFPPGTDPNVVACFQMADQDGSGFIDDKELQGALSSYNQKFSLRTVHLLMYLFTNSNNRKIGPKEFTAVFYSLQNWRAIFEKFDRDRSGKIEAGELREALMSLGFAVSPVVLDLLVSKFDKSGGRNKAIEYDNFIECCLTVKGLTEKFKEKDSSYSGSATFTYENFMLTVLPFLIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DWV1</accession>
    <name>A0AA39DWV1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PVL29_007877</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9699024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.10.10:FF:000007">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 17.6-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.370:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 17.6-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000020">
        <property type="entry name" value="Transposon Tf2-6 polyprotein-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005162">
        <property type="entry name" value="Retrotrans_gag_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041373">
        <property type="entry name" value="RT_RNaseH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056924">
        <property type="entry name" value="SH3_Tf2-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03732">
        <property type="entry name" value="Retrotrans_gag"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17917">
        <property type="entry name" value="RT_RNaseH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08284">
        <property type="entry name" value="RVP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24626">
        <property type="entry name" value="SH3_Tf2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="11" id="KW-0229">DNA integration</keyword>
    <keyword evidence="15" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="13" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="8" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="18">
        <location>
            <begin position="588"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase catalytic" evidence="19">
        <location>
            <begin position="1111"/>
            <end position="1276"/>
        </location>
    </feature>
    <feature type="domain" description="Chromo" evidence="17">
        <location>
            <begin position="1421"/>
            <end position="1473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="212"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="284"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="8"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="35"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="284"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="324"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAJ9699024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1473" mass="170178" checksum="1EE7BCBDD19036E0" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DYZ6</accession>
    <name>A0AA39DYZ6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">LysM domain receptor-like kinase 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_004942</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DYZ6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00118">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000468">
        <property type="entry name" value="PTI1-like tyrosine-protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.350.10">
        <property type="entry name" value="LysM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018392">
        <property type="entry name" value="LysM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056562">
        <property type="entry name" value="LysM2_CERK1_LYK3_4_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056563">
        <property type="entry name" value="LysM3_LYK4_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036779">
        <property type="entry name" value="LysM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056561">
        <property type="entry name" value="NFP_LYK_LysM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052611">
        <property type="entry name" value="Plant_RLK_LysM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45927:SF11">
        <property type="entry name" value="LYSM DOMAIN RECEPTOR-LIKE KINASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45927">
        <property type="entry name" value="LYSM-DOMAIN RECEPTOR-LIKE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23446">
        <property type="entry name" value="LysM1_NFP_LYK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23472">
        <property type="entry name" value="LysM2_CERK1_LYK3_4_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23473">
        <property type="entry name" value="LysM3_LYK4_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51782">
        <property type="entry name" value="LYSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041406235" description="LysM domain receptor-like kinase 4" evidence="12">
        <location>
            <begin position="24"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="268"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="LysM" evidence="14">
        <location>
            <begin position="185"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="279"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="239"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="243"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9703361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="637" mass="69902" checksum="61121CA1EAD51C3C" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E1M1</accession>
    <name>A0AA39E1M1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_004929</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E1M1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9703330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="157" mass="16314" checksum="DB4C1A6400943590" modified="2024-01-24" version="1">MSMDSSSMEHSSGSNSVIYSGGGAADGSAATGGSGTGSFQGVGYGSNIGLVMPISTVIAHESGHGQGNGSFGDSKAKAISYDNIFGSTDPYHARSLYYLSQQSSAGMVKGSSAYDQGSGCNNWVPALAPLTTWQYAMEHLHSQYGMIHKAWKKGRRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E567</accession>
    <name>A0AA39E567_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_002517</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E567"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34452">
        <property type="entry name" value="MYOSIN HEAVY CHAIN-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34452:SF1">
        <property type="entry name" value="SPORULATION-SPECIFIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9707514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="293" mass="32943" checksum="525B08FC46BFEE73" modified="2024-01-24" version="1">MNTEQNRCDGSNGGKALSSKETVNEYMDKVNARVRFKPESTKLLSLEEEGGLNEEYSDSATGFDGSFNTSESLCAVKHDTSSTHEIDSLKSTISGDLNGLSYTQSQKGDPSDQRFLAQGSNDWIHGWSLDYYVDSDLEVVKSSIIELKLEVSSLQSHADEIGVETQKFAKQFTAEIASGEVLAEEVSVLKLECSKLKEDLEHLRNSKSISEFASWQIIRTDQDHGFKESQLRWQKGFLNMEDETRRRFLQPDLEALLHVLQDRKQGTRQAISMVRNNKHNGKIQREGAKVQGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E7F8</accession>
    <name>A0AA39E7F8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Hyaluronan/mRNA-binding protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_002122</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E7F8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039764">
        <property type="entry name" value="HABP4/SERBP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006861">
        <property type="entry name" value="HABP4_PAIRBP1-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019084">
        <property type="entry name" value="STM1-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12299:SF17">
        <property type="entry name" value="AT19571P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12299">
        <property type="entry name" value="HYALURONIC ACID-BINDING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04774">
        <property type="entry name" value="HABP4_PAI-RBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09598">
        <property type="entry name" value="Stm1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01233">
        <property type="entry name" value="HABP4_PAI-RBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hyaluronan/mRNA-binding protein" evidence="4">
        <location>
            <begin position="157"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="319"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="42"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="94"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="114"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="137"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="153"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="190"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="200"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9707002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="364" mass="39280" checksum="8176076E3462B954" modified="2024-01-24" version="1">MATTNPFDLLGDDDNDDPSQLASHLLKLAPAKKSSTPPPQAAPAQQAKPAKLPSKPLPPAQAVRETKNEVGRGGGRGGGRGYGRGRGGAGLNRDSFNNESTFNNNDGFSGGYRPSEEGDTGKSSERRGSGGPRAPYRGGGGRRGGFSNGEVGEGERPRRAYERRSGTGRGNEIKRDGAGRGNWGTPADEIAPENEEPINETEKNTSAEKQLGEENAADANKENPVNESEEKEPEDKEMTLEEYEKVLEEKRKALLALKTEERKVDVKDFEAMQQLSSKKDNDDVFIKLGSEKDKRKELADKEERAKKSVSINEFLKPADGERYYGGRGRGRGRGTRGGFAGNTYNNVAAPSIEDPGQFPTLGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E8T1</accession>
    <name>A0AA39E8T1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_001440</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E8T1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22159">
        <property type="entry name" value="F-box_AtTIR1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000006">
        <property type="entry name" value="Transport inhibitor response 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041567">
        <property type="entry name" value="COI1_F-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041101">
        <property type="entry name" value="Transp_inhibit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16134">
        <property type="entry name" value="F-BOX/TPR REPEAT PROTEIN POF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16134:SF45">
        <property type="entry name" value="PROTEIN AUXIN SIGNALING F-BOX 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18511">
        <property type="entry name" value="F-box_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18791">
        <property type="entry name" value="Transp_inhibit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="COI1 F-box" evidence="1">
        <location>
            <begin position="10"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="Transport inhibitor response 1" evidence="2">
        <location>
            <begin position="68"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9709959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="588" mass="65534" checksum="49A4B2D5CE435158" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YHX5</accession>
    <name>A0AA38YHX5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CTLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_026979</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YHX5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024964">
        <property type="entry name" value="CTLH/CRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006595">
        <property type="entry name" value="CTLH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050618">
        <property type="entry name" value="Ubq-SigPath_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12864">
        <property type="entry name" value="RAN BINDING PROTEIN 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10607">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00668">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50897">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CTLH" evidence="2">
        <location>
            <begin position="48"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="CTLH" evidence="2">
        <location>
            <begin position="465"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="376"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="634"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="376"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="398"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="634"/>
            <end position="654"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9670774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="712" mass="79256" checksum="877ED9C0BAAF1E26" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YHY4</accession>
    <name>A0AA38YHY4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_027000</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the peptidase A1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YHY4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000012">
        <property type="entry name" value="Aspartyl protease family protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000014">
        <property type="entry name" value="Aspartyl protease family protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032799">
        <property type="entry name" value="TAXi_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032861">
        <property type="entry name" value="TAXi_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13683:SF743">
        <property type="entry name" value="ASPARTIC PROTEINASE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13683">
        <property type="entry name" value="ASPARTYL PROTEASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14541">
        <property type="entry name" value="TAXi_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14543">
        <property type="entry name" value="TAXi_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 13" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041424815" description="Peptidase A1 domain-containing protein" evidence="15">
        <location>
            <begin position="22"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="16">
        <location>
            <begin position="100"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="451"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="460"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="476"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="118"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9670801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="530" mass="58081" checksum="5F2502517D18E960" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YI21</accession>
    <name>A0AA38YI21_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Cytochrome P450 CYP736A12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_027018</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YI21"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11072">
        <property type="entry name" value="CYP71-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000011">
        <property type="entry name" value="Cytochrome P450 83B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47943:SF2">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47943">
        <property type="entry name" value="CYTOCHROME P450 93A3-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10 11" id="KW-0349">Heme</keyword>
    <keyword evidence="7 10 11" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 11" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="10">
        <location>
            <position position="434"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9670829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="494" mass="56001" checksum="FF77DBBDFB57050B" modified="2024-01-24" version="1">MAWIWTALALIAIVFLFNMMKNKHKRLPPGPRGIPILGNMHMLGNLPHRALQALAKKYGPIMYMRLGFVPAIVVSSPQAAEQFLKTHDLVFANRPPHECSRHMLYDGKGISFSGYGPYWRSMRKLCTLELLTSHKINSFKPMRREEVGLLIKSFEEAARAGAAVDVSAKVALLTADMSCRMVFGKKYMEKDFDERGFKAVIQEAMQLAATPNIGDYIPCLLGLDLQGLTRRIKATGKVFDDFVEKIIDEHIHKPKEEGQTKDLVDVMLDLMGSEENEYNIQRANIKAISLDMMGGSMDTSATTIEWALSELIKHPPMMKKVRNELEKVVGMERMVEESDLETLEYLNMVVKETLRLHPVGPLLLPHESMEDCTVDGFHIPQKSRVIVNVWAIGRDPNAWTDADKFLPERFMESDIDFRGQHFQFIPFGSGRRGCPGMQLGLTLVRLVLAQLVHCFDWELPDNMLPSELDMTEEFGLTLPRAKHLVAIPTCRLHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YI34</accession>
    <name>A0AA38YI34_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">PVL29_027028</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="24">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YI34"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000015">
        <property type="entry name" value="Somatic embryogenesis receptor kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000024">
        <property type="entry name" value="Somatic embryogenesis receptor kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000016">
        <property type="entry name" value="Somatic embryogenesis receptor-like kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47988">
        <property type="entry name" value="SOMATIC EMBRYOGENESIS RECEPTOR KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10 22" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041421728" description="non-specific serine/threonine protein kinase" evidence="22">
        <location>
            <begin position="26"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="301"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="329"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KAJ9670844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="624" mass="68758" checksum="0ABF1B1DAD9C0A5B" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YJD3</accession>
    <name>A0AA38YJD3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_025312</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YJD3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021434">
        <property type="entry name" value="DUF3082"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35733">
        <property type="entry name" value="OS02G0307800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35733:SF1">
        <property type="entry name" value="OS02G0307800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11282">
        <property type="entry name" value="DUF3082"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="154"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="195"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="237"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="247"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9671550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="277" mass="29842" checksum="3FEC51A9ED8D9ABD" modified="2024-01-24" version="1">MLQSSQRLFSSFSLPLSPSHNPCLSPPFYSLSRPTSLHLVSAHLRTRAEPLLAQVPEDTTTAPEEGPIELPPSSSSIFATTDDPTPLQVATSVLLTGAISVFLFRSIRRRVKRAKELRLRSSGVKKTLKEEALDSLKAMGSGSVKAAPPSPVQALLGGITAGVIALILYKFTITIEASLNRQTMSDSFSVRQITITIRTIINGLCYLATFVFGINSVGLFLYSGQLALNSFMEGSTSEETQIKGEEQLSTPNTLADSPPDGTQLNSSEGDQRAQDEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YKY6</accession>
    <name>A0AA38YKY6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Pectinesterase</fullName>
            <ecNumber evidence="4 12">3.1.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_025830</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10 12">
            <text>[(1-&gt;4)-alpha-D-galacturonosyl methyl ester](n) + n H2O = [(1-&gt;4)-alpha-D-galacturonosyl](n) + n methanol + n H(+)</text>
            <dbReference type="Rhea" id="RHEA:22380"/>
            <dbReference type="Rhea" id="RHEA-COMP:14570"/>
            <dbReference type="Rhea" id="RHEA-COMP:14573"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17790"/>
            <dbReference type="ChEBI" id="CHEBI:140522"/>
            <dbReference type="ChEBI" id="CHEBI:140523"/>
            <dbReference type="EC" id="3.1.1.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the pectinesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.11" evidence="4 12"/>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YKY6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030599">
        <property type="term" value="F:pectinesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042545">
        <property type="term" value="P:cell wall modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045490">
        <property type="term" value="P:pectin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000008">
        <property type="entry name" value="Pectinesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033131">
        <property type="entry name" value="Pectinesterase_Asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000070">
        <property type="entry name" value="Pectinesterase_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31321">
        <property type="entry name" value="ACYL-COA THIOESTER HYDROLASE YBHC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31321:SF31">
        <property type="entry name" value="PECTINESTERASE QRT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01095">
        <property type="entry name" value="Pectinesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00503">
        <property type="entry name" value="PECTINESTERASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0063">Aspartyl esterase</keyword>
    <keyword evidence="5" id="KW-0134">Cell wall</keyword>
    <keyword evidence="8 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041481425" description="Pectinesterase" evidence="12">
        <location>
            <begin position="27"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="Pectinesterase catalytic" evidence="13">
        <location>
            <begin position="61"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9672363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="359" mass="39407" checksum="1BE6BE476795D7EA" modified="2024-01-24" version="1">MGFSGFSASLCVVGLILVLSGGGCEGKANDNLRFITWSDFTARRDRPLLTVEGRNNTTKVIVVDKNGGGDSDTVQGAVDMVPVQNKQRVKIQIRPGIYREKVYVPASKPYISFIGNQIRSDDVVITWHDKASDLDSNGFRLGTVRTASVTVESDYFCAAGITIENSVVARPGVPGMQAVALNINGDKAMFYNVRLLGAQDTLMDLSGTHYFNQCYIQGSIDFIFGGARSIYQGCVIESIATTSGAIAAHRMESPNDGTGFSFVNCTIIGTGKIYLGRAWGEYSTAVYSNCRIADMVTPSGWSDWNKPERRRTAMFAEFNNTGKGADRSRRVKWSKSLRLEEAMPFMDLNFIAAEKWLRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YLE1</accession>
    <name>A0AA38YLE1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">S-protein homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_025996</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the plant self-incompatibility (S1) protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YLE1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060320">
        <property type="term" value="P:rejection of self pollen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010264">
        <property type="entry name" value="Self-incomp_S1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31232">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31232:SF149">
        <property type="entry name" value="S-PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05938">
        <property type="entry name" value="Self-incomp_S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 6" id="KW-0713">Self-incompatibility</keyword>
    <keyword evidence="5 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041482286" description="S-protein homolog" evidence="6">
        <location>
            <begin position="24"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9672598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="171" mass="19968" checksum="29A082D9912976C2" modified="2024-01-24" version="1">MFPFSRYLFSFVLLVLLVRLCDAGLGVIEKKVNLRITNDLATGADLNVHCKSKDDDLGIRVLAPNQFFEFRFRPNFWGTTLYFCKFWWGGESHWFDIYVEKRDTSRCDSKCWWMVGPAGPCLLDRRFGIYELCENWNDPQFEGWSKKGTGNDAARVHNDSKYLEGNEKRGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YM98</accession>
    <name>A0AA38YM98_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SKP1 component POZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_026367</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SKP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9673056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YM98"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009867">
        <property type="term" value="P:jasmonic acid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016897">
        <property type="entry name" value="SKP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001232">
        <property type="entry name" value="SKP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016073">
        <property type="entry name" value="Skp1_comp_POZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11165">
        <property type="entry name" value="SKP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03931">
        <property type="entry name" value="Skp1_POZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00512">
        <property type="entry name" value="Skp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="SKP1 component POZ" evidence="4">
        <location>
            <begin position="49"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9673056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="157" mass="17477" checksum="2B2D91460729B50B" modified="2024-01-24" version="1">MNQFSLSLSLSLSLSLSLYIYIYVDINPHRSSLLPFFFFSQSSPTLTKIVTLKSSDGQTFAVDEVVALKSPTIKNILEDIGAVENNAVSVPSVDGKTLAKVIEYCNEHAKEPSGFDEKAEVDEMEKWDAEFVDLLDREFLFDVLSPHVVSTFTTMKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YQ36</accession>
    <name>A0AA38YQ36_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_023865</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YQ36"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20654">
        <property type="entry name" value="CYP82"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000026">
        <property type="entry name" value="Cytochrome P450 82C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050651">
        <property type="entry name" value="Plant_Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47947:SF39">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47947">
        <property type="entry name" value="CYTOCHROME P450 82C3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0349">Heme</keyword>
    <keyword evidence="5 7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="7">
        <location>
            <position position="433"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9674580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="491" mass="55585" checksum="4AF843CF76C72A62" modified="2024-01-24" version="1">MYFLLQYLNFTTVGVFATLLLSYCLLLWRSRAGNSKIAPEATATWPIIDHLHLLGGSDQLPHITLGNMADKYGPVFTIRLGVHRALVVSSWEMAKECLTTNDLAASSRPQLLASKHLGYNYAMFGFSPYGSYWRELLKDVRASEVVTSIKELYKLWTEKKNESGLVSVEMKQWFGDLTLNVILRMVAGKRYFSASDASENKQAQRCRRVFREFSHLSGLFATEKTLKKTAKEMDSIAQEWLEEHRRRKDSGKDNSTQDFMDVMLSVLDGKNLGDYDADTINKATCLALIVGGSDTTVVTLTWELSLLLNNRDTLKKAQEELDHIQVGKKRLVNEQDISKLVYLQAIVKETLRLYLPVTLGGPRQFTEDCTLGGYHVSKGTRLILNLSKIQKDPRIWLGPTEFQPERFLTTHKDLDPRGKYFEFIPFGIGRRACTGIAFALQMLHLTLANFLQAFDFSTPSNALVDMRESLGLTNMKSTPIEVLISPRMSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YQJ0</accession>
    <name>A0AA38YQJ0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_023955</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YQJ0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16664">
        <property type="entry name" value="RING-Ubox_PUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:001480">
        <property type="entry name" value="RING-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.20">
        <property type="entry name" value="Adaptor protein Cbl, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036537">
        <property type="entry name" value="Adaptor_Cbl_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058678">
        <property type="entry name" value="ARM_PUB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045210">
        <property type="entry name" value="RING-Ubox_PUB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23315:SF239">
        <property type="entry name" value="RING-TYPE E3 UBIQUITIN TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23315">
        <property type="entry name" value="U BOX DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25598">
        <property type="entry name" value="ARM_PUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04564">
        <property type="entry name" value="U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51698">
        <property type="entry name" value="U_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="U-box" evidence="6">
        <location>
            <begin position="253"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9674732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="741" mass="83360" checksum="8ED3781497AE3175" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YU73</accession>
    <name>A0AA38YU73_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">O-fucosyltransferase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_021954</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycan metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase GT106 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9676685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YU73"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006004">
        <property type="term" value="P:fucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11299">
        <property type="entry name" value="O-FucT_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024709">
        <property type="entry name" value="FucosylTrfase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019378">
        <property type="entry name" value="GDP-Fuc_O-FucTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31741:SF63">
        <property type="entry name" value="O-FUCOSYLTRANSFERASE 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31741">
        <property type="entry name" value="OS02G0726500 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10250">
        <property type="entry name" value="O-FucT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009360">
        <property type="entry name" value="UCP009360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="11" id="KW-0294">Fucose metabolism</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="50"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9676685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="562" mass="62983" checksum="5BEF8B85B744D718" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YU91</accession>
    <name>A0AA38YU91_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Conserved oligomeric Golgi complex subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Component of oligomeric Golgi complex 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_021928</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the COG2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9676652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YU91"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017119">
        <property type="term" value="C:Golgi transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009316">
        <property type="entry name" value="COG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024603">
        <property type="entry name" value="COG_complex_COG2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024602">
        <property type="entry name" value="COG_su2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12961">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX COMPONENT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12961:SF0">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12022">
        <property type="entry name" value="COG2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06148">
        <property type="entry name" value="COG2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Conserved oligomeric Golgi complex subunit 2 N-terminal" evidence="9">
        <location>
            <begin position="22"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="COG complex component COG2 C-terminal" evidence="10">
        <location>
            <begin position="405"/>
            <end position="700"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF06148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF12022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9676652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="738" mass="82405" checksum="42193657AC6BF4BB" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YVV3</accession>
    <name>A0AA38YVV3_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_022470</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YVV3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008581">
        <property type="entry name" value="DUF863_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33167:SF18">
        <property type="entry name" value="GB|AAF67766.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33167">
        <property type="entry name" value="TRANSCRIPTION FACTOR, PUTATIVE (DUF863)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05904">
        <property type="entry name" value="DUF863"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="261"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="375"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="553"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="261"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="300"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="564"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="582"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="617"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="627"/>
            <end position="637"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9677492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="637" mass="70037" checksum="C8BDD63AC37E2007" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YZH1</accession>
    <name>A0AA38YZH1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_021354</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YZH1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000383">
        <property type="entry name" value="Leucine-rich repeat receptor protein kinase EMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000111">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046956">
        <property type="entry name" value="RLP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063">
        <property type="entry name" value="LRR RECEPTOR-LIKE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063:SF98">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE FLS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041306888" description="Leucine-rich repeat-containing N-terminal plant-type domain-containing protein" evidence="13">
        <location>
            <begin position="22"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="833"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="14">
        <location>
            <begin position="29"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="15">
        <location>
            <begin position="200"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9679397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="915" mass="102377" checksum="8F29D2D0A620D495" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z0N2</accession>
    <name>A0AA38Z0N2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">NB-ARC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_019389</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z0N2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056789">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766:SF55">
        <property type="entry name" value="OS11G0492900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766">
        <property type="entry name" value="PLANT BROAD-SPECTRUM MILDEW RESISTANCE PROTEIN RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25019">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NB-ARC" evidence="5">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="6">
        <location>
            <begin position="232"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="7">
        <location>
            <begin position="338"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="R13L1/DRL21-like LRR repeat region" evidence="8">
        <location>
            <begin position="485"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="924"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="925"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="944"/>
            <end position="953"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF25019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9680090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="979" mass="110057" checksum="B8D2E807DE4B1989" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z145</accession>
    <name>A0AA38Z145_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF4219 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_019699</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z145"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025314">
        <property type="entry name" value="DUF4219"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35317:SF11">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35317">
        <property type="entry name" value="OS04G0629600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13961">
        <property type="entry name" value="DUF4219"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4219" evidence="1">
        <location>
            <begin position="13"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9680448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="94" mass="10597" checksum="F40AC7656910A9D8" modified="2024-01-24" version="1">MEESSLTTAPSILDGNNYETWDVRMTVHLQALDVWEAVEENYEVPPLGANPTVTQMKLHKERKTRNAKVKACLFATISPSILIKIMKIDLAVEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z1B2</accession>
    <name>A0AA38Z1B2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_019557</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z1B2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF78">
        <property type="entry name" value="OS12G0587133 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="331"/>
            <end position="609"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9680278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1060" mass="122582" checksum="A4EA855653972F3F" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z365</accession>
    <name>A0AA38Z365_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_020421</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9681541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z365"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9681541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="131" mass="14745" checksum="6570CAB39E73CED6" modified="2024-01-24" version="1">MPFMSTAHFTISHHPSYLFADSPCTSFLTFMLPMSIISPKQLSYPDKPIIIPTAHALKPFYHPSNPLHFHSYLSIPTYLLLTHLSDHPCFHSLPIPMRGHPPLFMPIPPPFISPPFFLSCSSIKPITVPSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z5Y7</accession>
    <name>A0AA38Z5Y7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Hexosyltransferase</fullName>
            <ecNumber evidence="13">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_018840</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2 13">Glycan metabolism; pectin biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Golgi apparatus membrane</location>
            <topology evidence="1 13">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the glycosyltransferase 8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="13"/>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z5Y7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047262">
        <property type="term" value="F:polygalacturonate 4-alpha-galacturonosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06429">
        <property type="entry name" value="GT8_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000056">
        <property type="entry name" value="Hexosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029993">
        <property type="entry name" value="GAUT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002495">
        <property type="entry name" value="Glyco_trans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32116">
        <property type="entry name" value="GALACTURONOSYLTRANSFERASE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32116:SF61">
        <property type="entry name" value="GALACTURONOSYLTRANSFERASE 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01501">
        <property type="entry name" value="Glyco_transf_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 13" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 13" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9683011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="544" mass="62038" checksum="7A1100034D3954B4" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z5Z6</accession>
    <name>A0AA38Z5Z6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_018758</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z5Z6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005504">
        <property type="term" value="F:fatty acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009627">
        <property type="term" value="P:systemic acquired resistance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04660">
        <property type="entry name" value="nsLTP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.110.10">
        <property type="entry name" value="Plant lipid-transfer and hydrophobic proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036312">
        <property type="entry name" value="Bifun_inhib/LTP/seed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016140">
        <property type="entry name" value="Bifunc_inhib/LTP/seed_store"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039265">
        <property type="entry name" value="DIR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044741">
        <property type="entry name" value="NsLTP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33122">
        <property type="entry name" value="LIPID BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33122:SF60">
        <property type="entry name" value="LIPID-TRANSFER PROTEIN DIR1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14368">
        <property type="entry name" value="LTP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00499">
        <property type="entry name" value="AAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47699">
        <property type="entry name" value="Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9682894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="119" mass="12471" checksum="B4359CDB231D1C4A" modified="2024-01-24" version="1">MDLGMLGSSPTYRSYPSTDNMEAYKRFVILALVLVVAVVANGQGSICRMTQDGLTACKPSVSGQSPLPPSPACCAAISKADLPCLCSFKNSALLPYLGIDPNMATQLPAKCNIVTSTHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z6Q8</accession>
    <name>A0AA38Z6Q8_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_019040</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z6Q8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051165">
        <property type="entry name" value="Multifunctional_ANK_Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24123:SF139">
        <property type="entry name" value="ANKYRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24123">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13606">
        <property type="entry name" value="Ank_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="87"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="311"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="348"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="485"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="552"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="585"/>
            <end position="617"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9683282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="761" mass="82377" checksum="6FC706C482EDA07C" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z6S2</accession>
    <name>A0AA38Z6S2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_018873</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z6S2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010038">
        <property type="term" value="P:response to metal ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.430:FF:000003">
        <property type="entry name" value="FERONIA receptor-like kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.430:FF:000007">
        <property type="entry name" value="FERONIA receptor-like kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000252">
        <property type="entry name" value="Receptor-like protein kinase FERONIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000645">
        <property type="entry name" value="Receptor-like protein kinase FERONIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.430">
        <property type="entry name" value="Galactose-binding lectin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045272">
        <property type="entry name" value="ANXUR1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024788">
        <property type="entry name" value="Malectin-like_Carb-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34590">
        <property type="entry name" value="OS03G0124300 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34590:SF5">
        <property type="entry name" value="OS04G0586500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12819">
        <property type="entry name" value="Malectin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 15" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041329974" description="Protein kinase domain-containing protein" evidence="15">
        <location>
            <begin position="23"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="441"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="521"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="51"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="413"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="817"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="60"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="827"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="849"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="876"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="550"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9683068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="887" mass="98457" checksum="C9D4A491F1599865" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z7L9</accession>
    <name>A0AA38Z7L9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Cytochrome P450 90A1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_016349</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Hormone biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="13">Plant hormone biosynthesis; brassinosteroid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9683809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z7L9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016132">
        <property type="term" value="P:brassinosteroid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010268">
        <property type="term" value="P:brassinosteroid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016125">
        <property type="term" value="P:sterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11043">
        <property type="entry name" value="CYP90-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286:SF44">
        <property type="entry name" value="3BETA,22ALPHA-DIHYDROXYSTEROID 3-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286">
        <property type="entry name" value="CYTOCHROME P450 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 14 15" id="KW-0349">Heme</keyword>
    <keyword evidence="10 14 15" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 15" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="binding site" description="axial binding residue" evidence="14">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9683809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="364" mass="41749" checksum="F85065ABD2CFBD3F" modified="2024-01-24" version="1">MKGNLHKKMHSLTMSFGNSSIIKDHLLLDIDRLIRFNMDSWTSRILLMEEAKKITFDLTVKQLMSFDPGEWTESLRKEYVLVIEGFFTVPFPLFSATYRRAIQARTKVAEALNLVVRERRKAKEEGEEEKKNDMLAALLDSGDNFSDEQIVDFILALLVAGYETTSTIMTLAIKFLTETPLALAQLREEHDEIRARISDPNVLEWSDYKSMPFTQCVVNETLRVANIIGGIFRRVTTDIHVKGYTIPKGWKVFASFRAVHLDHEYFKDARTFNPWRWQNNSGPTVPGSGVNVFTPFGGGPRLCPGYELARVVLSVFLHRMVTRYNWAPAEEDKLVFFPTTRTQKRYPINVERRKVSSAAEKGEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z8D4</accession>
    <name>A0AA38Z8D4_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_016567</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z8D4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007750">
        <property type="entry name" value="DUF674"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33103">
        <property type="entry name" value="OS01G0153900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33103:SF19">
        <property type="entry name" value="OS09G0544700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05056">
        <property type="entry name" value="DUF674"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9684137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="127" mass="14283" checksum="8D9A32481A2671F5" modified="2024-01-24" version="1">MALRLVRQITFFQILEKAEEGYVKGVVTYMVTDALVVKPLSTMSIVDLLNKPNINEVGGELEETVVDFTMLEGLKLLMASMQTKQSLANGFLCKILFGFMIGFEAAEEAWLCEIFFENIAYTIAKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z8M6</accession>
    <name>A0AA38Z8M6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ABC1 atypical kinase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_016513</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z8M6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010287">
        <property type="term" value="C:plastoglobule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05121">
        <property type="entry name" value="ABC1_ADCK3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004147">
        <property type="entry name" value="ABC1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051130">
        <property type="entry name" value="Mito_struct-func_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43173">
        <property type="entry name" value="ABC1 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43173:SF22">
        <property type="entry name" value="OS07G0227800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03109">
        <property type="entry name" value="ABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC1 atypical kinase-like" evidence="1">
        <location>
            <begin position="166"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9684058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="539" mass="59901" checksum="69E82B1382223132" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z944</accession>
    <name>A0AA38Z944_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">Laccase</fullName>
            <ecNumber evidence="4 13">1.10.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Benzenediol:oxygen oxidoreductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Diphenol oxidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Urishiol oxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_016670</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Lignin degradation and detoxification of lignin-derived products.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>4 hydroquinone + O2 = 4 benzosemiquinone + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:11276"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17594"/>
            <dbReference type="ChEBI" id="CHEBI:17977"/>
            <dbReference type="EC" id="1.10.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="13">Binds 4 Cu cations per monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Secreted</location>
            <location evidence="2 13">Extracellular space</location>
            <location evidence="2 13">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the multicopper oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.10.3.2" evidence="4 13"/>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z944"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052716">
        <property type="term" value="F:hydroquinone:oxygen oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046274">
        <property type="term" value="P:lignin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13849">
        <property type="entry name" value="CuRO_1_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13875">
        <property type="entry name" value="CuRO_2_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13897">
        <property type="entry name" value="CuRO_3_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000049">
        <property type="entry name" value="Laccase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000062">
        <property type="entry name" value="Laccase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033138">
        <property type="entry name" value="Cu_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002355">
        <property type="entry name" value="Cu_oxidase_Cu_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034288">
        <property type="entry name" value="CuRO_1_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034285">
        <property type="entry name" value="CuRO_2_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034289">
        <property type="entry name" value="CuRO_3_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017761">
        <property type="entry name" value="Laccase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03389">
        <property type="entry name" value="laccase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF522">
        <property type="entry name" value="LACCASE-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00079">
        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00080">
        <property type="entry name" value="MULTICOPPER_OXIDASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0052">Apoplast</keyword>
    <keyword evidence="10 13" id="KW-0186">Copper</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 13" id="KW-0439">Lignin degradation</keyword>
    <keyword evidence="7 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 13" id="KW-0964">Secreted</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041483220" description="Laccase" evidence="13">
        <location>
            <begin position="32"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="16">
        <location>
            <begin position="43"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="14">
        <location>
            <begin position="167"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="15">
        <location>
            <begin position="438"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9684298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="583" mass="64222" checksum="8FB3C94186ADA54B" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2025-10-08" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZB76</accession>
    <name>A0AA38ZB76_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Clustered mitochondria protein homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_017738</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">mRNA-binding protein involved in proper cytoplasmic distribution of mitochondria.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CLU family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd15466">
        <property type="entry name" value="CLU-central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000230">
        <property type="entry name" value="Clustered mitochondria protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2280.10:FF:000002">
        <property type="entry name" value="Clustered mitochondria protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2280.10">
        <property type="entry name" value="Hypothetical protein (hspc210)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03013">
        <property type="entry name" value="CLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033646">
        <property type="entry name" value="CLU-central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025697">
        <property type="entry name" value="CLU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028275">
        <property type="entry name" value="CLU_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027523">
        <property type="entry name" value="CLU_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007967">
        <property type="entry name" value="GSKIP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023231">
        <property type="entry name" value="GSKIP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12601:SF6">
        <property type="entry name" value="CLUSTERED MITOCHONDRIA PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12601">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT EIF-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13236">
        <property type="entry name" value="CLU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15044">
        <property type="entry name" value="CLU_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12807">
        <property type="entry name" value="eIF3_p135"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05303">
        <property type="entry name" value="GSKIP_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103107">
        <property type="entry name" value="Hypothetical protein c14orf129, hspc210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51823">
        <property type="entry name" value="CLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="Clu" evidence="5">
        <location>
            <begin position="357"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="1152"/>
            <end position="1185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="476"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="699"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="903"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1375"/>
            <end position="1445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="15"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="476"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="735"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1391"/>
            <end position="1412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9685801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1445" mass="157297" checksum="E8C4B88B9DFF5F55" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZET3</accession>
    <name>A0AA38ZET3_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_015959</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZET3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9687282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="72" mass="7126" checksum="FC5095F2BB169164" modified="2024-01-24" version="1">MATLIGVGVGADPGARASTVSTTTWNMRDMGGGAASSPGLDRGSMSGTKTNSVRLAAIVGFSGLRRISSCHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZH94</accession>
    <name>A0AA38ZH94_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_014495</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZH94"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015145">
        <property type="term" value="F:monosaccharide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17361">
        <property type="entry name" value="MFS_STP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000002">
        <property type="entry name" value="Sugar transport protein 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044778">
        <property type="entry name" value="MFS_STP/MST-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045262">
        <property type="entry name" value="STP/PLT_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23500">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 2, FACILITATED GLUCOSE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23500:SF109">
        <property type="entry name" value="SUGAR TRANSPORT PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="6" id="KW-0769">Symport</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="25"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="86"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="205"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="288"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="322"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="352"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="386"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="427"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="454"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="11">
        <location>
            <begin position="32"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9688877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="511" mass="55736" checksum="9CACFC21ED7DFF99" modified="2024-01-24" version="1">MEVGDGSFAPVGVSKQRAELYKGRLTTYVTVACLVAAVGGAIFGYDIGISGGVTSMDTFLEKFFHTVYLKKRRAEEDHYCKYNDQGLAAFTSSLYLAGLVASIVASPITRKYGRRASIVCGGISFLIGATLNAAAVNLGMLLLGRIMLGVGIGFGDQAVPLYLSEMAPAHLRGALNMMFQLATTSGIFTANMINYGTAKLPSWGWRLSLGLAAFPAVIMTVGGLLLPETPNSLIERGSREKGRQVLERIRGTNEVDAEFEDIVDASELANSIKHPFRNILERRNRPQLVMAICMPAFQILNGINSILFYAPVLFETMGFGNASLYSSALTGAVLVLSTLVSIGLVDRLGRRVLLISGGIQMVFCQVIVAIILGVKFGGNDELSKGYSVLVVIVICLFVVAFGWSWGPLGWTVPSEIFPLETRSAGQSITVAVNLLFTFIIAQCFLSMLCAFKHGIFLFFAGWIIIMTLFVYFFLPETKGVPIEEMIFVWRKHWFWKRMVPGTPDVDDIDGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZKA5</accession>
    <name>A0AA38ZKA5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Mg-protoporphyrin IX chelatase</fullName>
            <ecNumber evidence="10">6.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_012993</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Involved in chlorophyll biosynthesis. Catalyzes the insertion of magnesium ion into protoporphyrin IX to yield Mg-protoporphyrin IX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>protoporphyrin IX + Mg(2+) + ATP + H2O = Mg-protoporphyrin IX + ADP + phosphate + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13961"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57306"/>
            <dbReference type="ChEBI" id="CHEBI:60492"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.6.1.1"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="10">Redox regulation; active in reducing conditions, inactive in oxidizing conditions.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Porphyrin-containing compound metabolism; chlorophyll biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">The magnesium chelatase complex is a heterotrimer consisting of subunits CHLI, CHLD and CHLH.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">The magnesium chelatase complex is a heterotrimer consisting of subunits CHLI, CHLD, AND CHLH.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Plastid</location>
            <location evidence="10">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the Mg-chelatase subunits D/I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.6.1.1" evidence="10"/>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZKA5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009570">
        <property type="term" value="C:chloroplast stroma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010007">
        <property type="term" value="C:magnesium chelatase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016851">
        <property type="term" value="F:magnesium chelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015995">
        <property type="term" value="P:chlorophyll biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015979">
        <property type="term" value="P:photosynthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.80:FF:000001">
        <property type="entry name" value="Mg-protoporphyrin IX chelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000601">
        <property type="entry name" value="Mg-protoporphyrin IX chelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.80">
        <property type="entry name" value="Magnesium chelatase subunit I, C-Terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045006">
        <property type="entry name" value="CHLI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041628">
        <property type="entry name" value="ChlI/MoxR_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011775">
        <property type="entry name" value="Mg_chelatase_ATPase-isu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000523">
        <property type="entry name" value="Mg_chelatse_chII-like_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02030">
        <property type="entry name" value="BchI-ChlI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32039">
        <property type="entry name" value="MAGNESIUM-CHELATASE SUBUNIT CHLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32039:SF9">
        <property type="entry name" value="MAGNESIUM-CHELATASE SUBUNIT CHLI-2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17863">
        <property type="entry name" value="AAA_lid_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01078">
        <property type="entry name" value="Mg_chelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 10" id="KW-0149">Chlorophyll biosynthesis</keyword>
    <keyword evidence="10" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="4 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 10" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="10" id="KW-0934">Plastid</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="11">
        <location>
            <begin position="113"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9690615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="426" mass="46496" checksum="6E8E1AC6C1E6A395" modified="2024-01-24" version="1">MAGIFGTSSSSTAILASRPLSSASKSSIPSLSLPLCTWRSRGRKLYGGVGVQLKKGRSQFHVAVSNVATEINPAEKAQKLAAEESQRPVYPFAAIVGQDEMKLCLLLNVIDPKIGGVMIMGDRGTGKSTTVRSLVDLLPEIRVVSGDPFNSDPEDPESMGMEVRESILKGEQLSIVMTKINMVDLPLGATEDRVCGTIDIEKALTEGVKAFEPGLLAKANRGILYVDEVNLLDDHLVDVLLDSAASGWNTVEREGISISHPARFILIGSGNPEEGELRPQLLDRFGMHAQVGTVRDAELRVKIVEERARFDKNPKEFRDSYKAEQEKLQQQISSARSDLSSVQIDHDLRVKISKVCSELNVDGLRGDIVTNRAAKALAALKGRDNVIAEDIATVIPNCLRHRLRKDPLESIDSGLLVIEKFYEVFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZKH5</accession>
    <name>A0AA38ZKH5_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_012904</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZKH5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9690485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="30" mass="3581" checksum="403F3F961C78311F" modified="2024-01-24" version="1">MKLLFQCPCCSCFCFLMPKKRKPKKEEKGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZLS5</accession>
    <name>A0AA38ZLS5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">WPP domain-associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_013396</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZLS5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037490">
        <property type="entry name" value="WAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33883">
        <property type="entry name" value="WPP DOMAIN-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33883:SF10">
        <property type="entry name" value="WPP DOMAIN-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="336"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="454"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="601"/>
            <end position="663"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9691204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="903" mass="102921" checksum="08C6D34375FDFA5A" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZMA7</accession>
    <name>A0AA38ZMA7_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_013791</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZMA7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9691713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="73" mass="8439" checksum="67695C83008FB9F9" modified="2024-01-24" version="1">MKIISWNIQGLGSRKKRRVVKDFLHHENSDVVMFQETKREVCDMRFISSVWTTRNKDWTSLSACGALGGILII</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZMY1</accession>
    <name>A0AA38ZMY1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein FLX-like 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_011237</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FLX family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZMY1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009908">
        <property type="term" value="P:flower development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040353">
        <property type="entry name" value="FLX/FLX-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33405">
        <property type="entry name" value="PROTEIN FLX-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33405:SF20">
        <property type="entry name" value="PROTEIN FLX-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0221">Differentiation</keyword>
    <keyword evidence="5" id="KW-0287">Flowering</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="97"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="167"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="233"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9692040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="328" mass="37117" checksum="681847B46832ADBB" modified="2024-01-24" version="1">MPLPVLMFWLHQKPMVMEFELFTVWVLERIKNCCESIESITSRELEMAGRNRFPRDAFDGRRGFPPEGPFLRGPPLPRLPPHPAMLEEEFEMQHAEIRRLFGENRRLVEDRMALQQELGVAKEELHRMNLAIGDIRAEQELHSRELIEKGLKLEADLRATEPLKNEAVQLRAEIQKLNNIRQDLAGQVQNLSQEVARLQADNKQIPLLRAEIEGLHQELMRARTAVDYEKKGNIELMEQRQAMEKNLVSMAREVEKLRAELASTDARPWGAGISGGSYGMKFGSPDGGFPAPYGDGYGAHLGAADKGPFYGSGSASWGGLEKPRATRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZN01</accession>
    <name>A0AA38ZN01_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Nodulation signaling pathway 1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_011172</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the GRAS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9691937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZN01"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005202">
        <property type="entry name" value="TF_GRAS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31636">
        <property type="entry name" value="OSJNBA0084A10.13 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03514">
        <property type="entry name" value="GRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50985">
        <property type="entry name" value="GRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="84"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Leucine repeat II (LRII)" evidence="3">
        <location>
            <begin position="330"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="SAW" evidence="3">
        <location>
            <begin position="465"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="VHIID" evidence="3">
        <location>
            <begin position="272"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="128"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9691937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="546" mass="60433" checksum="E343409780E7E9C2" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZNZ0</accession>
    <name>A0AA38ZNZ0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tail fiber</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_011606</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZNZ0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35288">
        <property type="entry name" value="TAIL FIBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35288:SF2">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="160"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9692616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="206" mass="22801" checksum="C34653E613EA6A62" modified="2024-01-24" version="1">MASSKKWASSISSIASLIYFILIIFQIPLFRVPCRTGICTTPIELTSSQLIASEIFPAVVVKTLLYPGAIANAIIKNTSIPSYNNLLNDYNLINVKEAPVTTDLQYLEVLAGSYFSVAGAFLGLLKYGRVSLFGMLIIIWGLVKEVILRKPANTSPLKSVYMYPAMSIAVVCAFSSIRRDVRRLMRSCRARRAAKPPWSSSKSKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZPB3</accession>
    <name>A0AA38ZPB3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_011499</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZPB3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.430:FF:000003">
        <property type="entry name" value="Probable disease resistance protein At5g66910"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057135">
        <property type="entry name" value="At4g27190-like_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050905">
        <property type="entry name" value="Plant_NBS-LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463:SF220">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23247">
        <property type="entry name" value="LRR_RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="130"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9692465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="878" mass="100814" checksum="E649E4A36D86CAAA" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZRH5</accession>
    <name>A0AA38ZRH5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Acid phosphatase 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_009513</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">May function as somatic storage protein during early seedling development.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the APS1/VSP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9693590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZRH5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045735">
        <property type="term" value="F:nutrient reservoir activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07535">
        <property type="entry name" value="HAD_VSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005519">
        <property type="entry name" value="Acid_phosphat_B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010028">
        <property type="entry name" value="Acid_phosphatase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014403">
        <property type="entry name" value="APS1/VSP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01675">
        <property type="entry name" value="plant-AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31284">
        <property type="entry name" value="ACID PHOSPHATASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31284:SF9">
        <property type="entry name" value="HAD SUPERFAMILY, SUBFAMILY IIIB ACID PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03767">
        <property type="entry name" value="Acid_phosphat_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002674">
        <property type="entry name" value="VSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0758">Storage protein</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041254163" description="Acid phosphatase 1" evidence="4">
        <location>
            <begin position="23"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR002674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9693590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="252" mass="28401" checksum="D56B9DD7F01BAEF2" modified="2024-01-24" version="1">MRGVREVVVQVVFLAICSMANAIKPCPRATVVRDEVSYCLSWRMSVEANNMAGWRTVPMQCLPYIEGYMIGGQYDRDMDFIADQILSYVKGIVLSDDGMDAWILDVDDTCISNLFYYKGKRFGCDPYDPKGFKAWALKGGCPAISAVLGLFDKLVESGFKVILLTGRDEETLGQVTIDNLHNQGFIGYERLILRSAEHKGQGAIQYKSGIRKQLVEEGYRIWGNVGDQWSDLQGDYKGNRTFKLPNPMYFVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZS77</accession>
    <name>A0AA38ZS77_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_012241</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9693389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZS77"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000129">
        <property type="entry name" value="Leucine-rich repeat receptor-like kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000221">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase PXL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001347">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001646">
        <property type="entry name" value="Putative inactive leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000905">
        <property type="entry name" value="Receptor-like protein kinase 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046956">
        <property type="entry name" value="RLP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063">
        <property type="entry name" value="LRR RECEPTOR-LIKE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063:SF16">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041387332" evidence="13">
        <location>
            <begin position="23"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1011"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="14">
        <location>
            <begin position="34"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="15">
        <location>
            <begin position="108"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="15">
        <location>
            <begin position="396"/>
            <end position="562"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9693389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1068" mass="119857" checksum="6C9FFAE11397B762" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZU64</accession>
    <name>A0AA38ZU64_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Sulfoquinovosidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_010709</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the glycosyl hydrolase 31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZU64"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06594">
        <property type="entry name" value="GH31_glucosidase_YihQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14752">
        <property type="entry name" value="GH31_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1760">
        <property type="entry name" value="glycosyl hydrolase (family 31)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048395">
        <property type="entry name" value="Glyco_hydro_31_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000322">
        <property type="entry name" value="Glyco_hydro_31_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052990">
        <property type="entry name" value="Sulfoquinovosidase_GH31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044112">
        <property type="entry name" value="YihQ_TIM-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007746">
        <property type="entry name" value="PRK10426.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46959">
        <property type="entry name" value="SULFOQUINOVOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46959:SF2">
        <property type="entry name" value="SULFOQUINOVOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01055">
        <property type="entry name" value="Glyco_hydro_31_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21365">
        <property type="entry name" value="Glyco_hydro_31_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase family 31 TIM barrel" evidence="4">
        <location>
            <begin position="428"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 31 C-terminal" evidence="5">
        <location>
            <begin position="767"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF21365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9695370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="872" mass="99756" checksum="52ED826786ABEF03" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZVK4</accession>
    <name>A0AA38ZVK4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Eukaryotic translation initiation factor 3 subunit C</fullName>
            <shortName evidence="4">eIF3c</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Eukaryotic translation initiation factor 3 subunit 8</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">eIF3 p110</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_010885</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the eIF-3 subunit C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZVK4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03002">
        <property type="entry name" value="eIF3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027516">
        <property type="entry name" value="EIF3C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008905">
        <property type="entry name" value="EIF3C_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058999">
        <property type="entry name" value="EIF3CL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13937">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITATION FACTOR 3, SUBUNIT 8 EIF3S8 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13937:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05470">
        <property type="entry name" value="eIF-3c_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26569">
        <property type="entry name" value="EIF3CL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="6">
        <location>
            <begin position="625"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="135"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="171"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="454"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="861"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="12"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="54"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="142"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="175"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="199"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="209"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="454"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="861"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="871"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="896"/>
            <end position="905"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9695622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="934" mass="105747" checksum="B796935D45F64E44" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZWJ2</accession>
    <name>A0AA38ZWJ2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">CCT-theta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_010910</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZWJ2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03341">
        <property type="entry name" value="TCP1_theta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000008">
        <property type="entry name" value="T-complex protein 1 subunit theta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012721">
        <property type="entry name" value="Chap_CCT_theta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02346">
        <property type="entry name" value="chap_CCT_theta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 8" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9695668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="545" mass="58903" checksum="3C40C04D2C486BB2" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZWK3</accession>
    <name>A0AA38ZWK3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">HTH myb-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_010918</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MYB-CC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZWK3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000002">
        <property type="entry name" value="Myb family transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025756">
        <property type="entry name" value="Myb_CC_LHEQLE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006447">
        <property type="entry name" value="Myb_dom_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046955">
        <property type="entry name" value="PHR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01557">
        <property type="entry name" value="myb_SHAQKYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31499">
        <property type="entry name" value="MYB FAMILY TRANSCRIPTION FACTOR PHL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31499:SF2">
        <property type="entry name" value="MYB-RELATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14379">
        <property type="entry name" value="Myb_CC_LHEQLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="8">
        <location>
            <begin position="43"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9695683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="418" mass="46355" checksum="D9551D310403880F" modified="2024-01-24" version="1">MYHHHHHQGKNIHPSSRTPITPERNLFLQGGNGPGDSGLVLSTDAKPRLKWTPDLHERFIEAVNQLGGADKATPKTVMKLMGIPGLTLYHLKSHLQKYRLSKNLHGQANSGTSKTVVGERMPEANGALMSSPNIGNQTNKSLHLSETLQMIEAQRRLHEQLEVQRHLQLRIEAQGKYLQAVLEKAQETLGRQNLGAVGLEAAKVQLSELVSKVSTQCLHSAFSELKELQSLCPQQTQTQPTDCSIDSCLTSCEGSQRDQEIHNCGMGLRPYTNGSTPLEAKDIAEPPGLQHAVLKWCEDTKENRQFISSMQRDAERRTMTAERSNSDLSMRIGLQGEKGNGSNSYSEGRFKGRAEADNFVDRTNHGADSGNSVKQENEKMSHGYRLPCFGAKLDLNAHDENDVTLSCKQFDLNGFSWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZWU3</accession>
    <name>A0AA38ZWU3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Stachyose synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_008753</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolases 36 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZWU3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008811">
        <property type="entry name" value="Glycosyl_hydrolases_36"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31268">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31268:SF8">
        <property type="entry name" value="GALACTINOL--SUCROSE GALACTOSYLTRANSFERASE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05691">
        <property type="entry name" value="Raffinose_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9696691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="873" mass="96974" checksum="3293ECFFCDB1B106" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZXX6</accession>
    <name>A0AA38ZXX6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Ribophorin-2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_008941</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the oligosaccharyltransferase (OST) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SWP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZXX6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008250">
        <property type="term" value="C:oligosaccharyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055375">
        <property type="entry name" value="Ribophorin_II_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055374">
        <property type="entry name" value="Ribophorin_II_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055373">
        <property type="entry name" value="Ribophorin_II_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008814">
        <property type="entry name" value="Swp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12640:SF0">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12640">
        <property type="entry name" value="RIBOPHORIN II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05817">
        <property type="entry name" value="Ribophorin_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23861">
        <property type="entry name" value="Ribophorin_II_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23860">
        <property type="entry name" value="Ribophorin_II_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041486133" description="Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2" evidence="1">
        <location>
            <begin position="25"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="domain" description="Ribophorin II N-terminal" evidence="2">
        <location>
            <begin position="27"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="Ribophorin II second" evidence="4">
        <location>
            <begin position="317"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="Ribophorin II third" evidence="3">
        <location>
            <begin position="432"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU366029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9696950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="505" mass="54851" checksum="34658B45B0F37256" modified="2024-01-24" version="1">MARSPGGFLVLVLILTISIHKAASILDSHQPVALELFSPVHGSFGSLEESYDALQTFQILGIEKKPDISDTTCTLVLKTLGSSSSSLKDLFYALKVNGILKCDIDEEVFEGIKSRLQSVSNDASSLLDFYYSIGSLALIKEQAPKVDVLLTDADGIFQSIKALSQSDGRWRYSSNNPESSTYAAALLNYSTLPLFPSLVTYGTCLLLVRLSICSASIHQGLDFTGWLFQVYDGAFYFDEKIVDAREQHGPLSTTASVVRGLTAFADVTSGSLNIPGDKILGLAKFFLSVGIPGDAKDFFNQVYSLACLESNRVSIPLILSLPATVLSLTKNDQLKVRVNTVLGSSAPPLTVKLLQAFSSSSKNAFIIENQELKFDPENAVHFLDALPENVDVGNYVFVFEILLHDPEHQKIYATGSRTRVPIIVTGVIKVDTAEIVILDSDLGSVETKRKLDLAGENDVSLSANHLQKLRLSFQLATPLGHAFKPHQVCGQLSVSCMIFFPCLVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A0A7</accession>
    <name>A0AA39A0A7_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_007537</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the short-chain dehydrogenases/reductases (SDR) family. SDR65C subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A0A7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05329">
        <property type="entry name" value="TR_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045000">
        <property type="entry name" value="TR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42898:SF6">
        <property type="entry name" value="NADP-DEPENDENT MANNITOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42898">
        <property type="entry name" value="TROPINONE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9698506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="270" mass="29080" checksum="D0E7BAD53DE6828C" modified="2024-01-24" version="1">MAQTFGCSSGDSRWSLKGMTALVTGGTKGVGHAIVEELAGLGATIHTCSRKETELNECLKDWKAKGFEVSGSVCDLSSRAQREKLMETVSSVFNGKLNILVNNAAIVIQKPTVEVTAEEFSTIMAINFESVYHLSQLAHPLLKASGDGSIVFISSVAGVVSLKYLSAYSVTKGAMNQLTKNLACEWAEDNIRSNAVAPWYIKTPMVDQMLSNKTFLEEVINRTPLRRVGDPKEVSSLVAFLCLPASSYITGQTICVDGGVTVNGFEPNLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A120</accession>
    <name>A0AA39A120_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Association with the SNF1 complex (ASC) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_007864</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 5'-AMP-activated protein kinase beta subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9699000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A120"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02859">
        <property type="entry name" value="E_set_AMPKbeta_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.250.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032640">
        <property type="entry name" value="AMPK1_CBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006828">
        <property type="entry name" value="ASC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037256">
        <property type="entry name" value="ASC_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043554">
        <property type="entry name" value="KINB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46316">
        <property type="entry name" value="SNF1-RELATED PROTEIN KINASE REGULATORY SUBUNIT BETA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46316:SF9">
        <property type="entry name" value="SNF1-RELATED PROTEIN KINASE REGULATORY SUBUNIT BETA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16561">
        <property type="entry name" value="AMPK1_CBM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04739">
        <property type="entry name" value="AMPKBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01010">
        <property type="entry name" value="AMPKBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160219">
        <property type="entry name" value="AMPKBI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Association with the SNF1 complex (ASC)" evidence="3">
        <location>
            <begin position="197"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="33"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9699000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="286" mass="31372" checksum="FE39CBB5130E2FFD" modified="2024-01-24" version="1">MGNANGREEGANGRDDSTARSNGDSGVRDIYAPSSTHPARVASSDSMGNTPPQSPGRSRSPLMFAPQVPIAPLQRRDGPPPLNQMWQNEPQGGVEHPPEQGIPIMIAWNYGGNDVAVEGSWDNWTSRKTLQRSGKDHSILLVLPSGVYHYKIIVDGEWRYIPDLPFIADEMGRVCNLLDVNDNVPENLESVAEFEAPPSPNSSYGQAFPAEEDFAKEPVLVPPQLHLTVLGMPNSEEASCSKPQHVVLNHLFIEKGWASQSVVALGLTNRFQSKYVTVVLYKPLKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A1S5</accession>
    <name>A0AA39A1S5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Transcription factor RAX2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_008066</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9699286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A1S5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000015">
        <property type="entry name" value="Transcription factor RAX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48000">
        <property type="entry name" value="OS09G0431300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48000:SF72">
        <property type="entry name" value="TRANSCRIPTION FACTOR RAX2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="8">
        <location>
            <begin position="9"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="7">
        <location>
            <begin position="9"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="8">
        <location>
            <begin position="63"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="7">
        <location>
            <begin position="63"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9699286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="318" mass="35573" checksum="DE3956D31F58FD96" modified="2024-01-24" version="1">MGRAPCCDKANVKKGPWSPEEDSKLKAYIEKYGTGGNWIALPQKAGLKRCGKSCRLRWLNYLRPNIKHGEFSDDEDRIICTLFANMGSRWSVIATRLPGRTDNDIKNYWNTKLKKKVMGMFSSSQRKLPPFPSVQTPPSPSISSSYYIPPSTFPGHELPIPFPSAHLNNTTSPLTNISLLQTQETFAGPGQYYPVKDNLFMFASEASCSSSDGSGSQISFGRDIKQEEMGNFQSYIPTGFEENHKFMMGYGGSNGGHGHNANHWPEKPNGCFGESPLDQYNLDQEDVKQQLISCSNGGCTSSFFIDENKTQEKVMYYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A2V0</accession>
    <name>A0AA39A2V0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Acetate/butyrate--CoA ligase AAE7, peroxisomal</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_005565</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9699754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A2V0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12118">
        <property type="entry name" value="ttLC_FACS_AEE21_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000008">
        <property type="entry name" value="2,3-dihydroxybenzoate-AMP ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000003">
        <property type="entry name" value="Long-chain-fatty-acid--CoA ligase FadD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006020">
        <property type="entry name" value="PRK08162.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43859:SF7">
        <property type="entry name" value="ACETATE_BUTYRATE--COA LIGASE AAE7, PEROXISOMAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43859">
        <property type="entry name" value="ACYL-ACTIVATING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="3">
        <location>
            <begin position="26"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="4">
        <location>
            <begin position="467"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9699754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="567" mass="62240" checksum="A8905FA67A5EE4F5" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A2Z1</accession>
    <name>A0AA39A2Z1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Enoyl-CoA hydratase 1, peroxisomal</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_005601</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9699814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A2Z1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000054">
        <property type="entry name" value="probable enoyl-CoA hydratase 1, peroxisomal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004840">
        <property type="entry name" value="PRK06190.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43802">
        <property type="entry name" value="ENOYL-COA HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43802:SF1">
        <property type="entry name" value="IP11341P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9699814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="266" mass="29239" checksum="61C174D783E4522D" modified="2024-01-24" version="1">MALSSSSEELILVNREPSGVAFVTINRPRLLNSLTRPMMVDMARAFKSLDADDSVRVIVITGSGRAFCSGVDLTAAEEVFKGDVKDVEIDPVVQMERCRKPIVGAIGGFAVTAGFEIALACDILVAVKGTKFIDTHARFGIFPSWGLSQKLSRIIGANRAREVSLTAMPVTAEQAERWGFVNHVVEGSELLKKAREVAEAIIKNNQDLVMRYKSVINDGLKLDLGHALALEKERAHNYYNGMTQEQFKKMQEFIAGRSTKKPSPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A4F4</accession>
    <name>A0AA39A4F4_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_006131</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A4F4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058580">
        <property type="entry name" value="DUF2828"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056690">
        <property type="entry name" value="DUF7788"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011205">
        <property type="entry name" value="UCP015417_vWA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31373">
        <property type="entry name" value="OS06G0652100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31373:SF27">
        <property type="entry name" value="TROVE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11443">
        <property type="entry name" value="DUF2828"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25043">
        <property type="entry name" value="DUF7788"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015417">
        <property type="entry name" value="T31B5_30_vWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2828" evidence="1">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7788" evidence="2">
        <location>
            <begin position="91"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF11443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9700666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="240" mass="26696" checksum="C5FE0B1E4D79BAE1" modified="2024-01-24" version="1">MCSNQWGSLPYNRAASMAMKSYKSLFSKHDTERFGEYLEKVQTGKAKIAAAGALLPHEIIASLNEKDGEKVAELQWARMVEELSEKGRLTNCISVCDVSGSMSGTPMEVCVALGLLVSELSEDPWKGNVITFSASPELHKIQGDSLVSKTEFVRMMEWGANTDFQKVFDRILQVAVEGNLSEDQMIKRVFVFSDMEFDEACGRYNHCEQVKPGILQDETRKDPGGILNNIPIMLVASLFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2025-04-02" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A4R6</accession>
    <name>A0AA39A4R6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">COP1-interacting protein 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_006175</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31008:SF2">
        <property type="entry name" value="COP1-INTERACTING PROTEIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31008">
        <property type="entry name" value="COP1-INTERACTING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="363"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="773"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1051"/>
            <end position="1074"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1196"/>
            <end position="1217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1262"/>
            <end position="1344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="403"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="428"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="462"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="504"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="782"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="816"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="867"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="916"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="977"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1064"/>
            <end position="1074"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1196"/>
            <end position="1208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1294"/>
            <end position="1321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1322"/>
            <end position="1335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9700729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1344" mass="147735" checksum="686E3EF7340F7EEC" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A6F6</accession>
    <name>A0AA39A6F6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein cereblon</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Protein ohgata</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PVL29_003876</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Has an essential role in mediating growth by negatively regulating insulin signaling. It also has a role in maintaining presynaptic function in the neuromuscular junction synapses of third-instar larvae.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Likely a component of a DCX (DDB1-CUL4-X-box) protein ligase complex. May interact with pic/DDB1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the 4-toluene sulfonate uptake permease (TSUP) (TC 2.A.102) family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CRBN family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9701850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A6F6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031464">
        <property type="term" value="C:Cul4A-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15777">
        <property type="entry name" value="CRBN_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.130.40:FF:000009">
        <property type="entry name" value="ATP-dependent protease La domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.150.20:FF:000005">
        <property type="entry name" value="Blast:Protein cereblon homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1480:FF:000007">
        <property type="entry name" value="Lon protease homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.40">
        <property type="entry name" value="LON domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.20">
        <property type="entry name" value="Peptide methionine sulfoxide reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034750">
        <property type="entry name" value="CULT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003111">
        <property type="entry name" value="Lon_prtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046336">
        <property type="entry name" value="Lon_prtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004910">
        <property type="entry name" value="Yippee/Mis18/Cereblon"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14255">
        <property type="entry name" value="CEREBLON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14255:SF4">
        <property type="entry name" value="PROTEIN CEREBLON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02190">
        <property type="entry name" value="LON_substr_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03226">
        <property type="entry name" value="Yippee-Mis18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00464">
        <property type="entry name" value="LON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51788">
        <property type="entry name" value="CULT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51787">
        <property type="entry name" value="LON_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Lon N-terminal" evidence="16">
        <location>
            <begin position="89"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="CULT" evidence="17">
        <location>
            <begin position="440"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="220"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="221"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00014394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00046075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00046796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ9701850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="548" mass="62219" checksum="E70307653D2B9FA3" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A7I9</accession>
    <name>A0AA39A7I9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Hemoglobin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_004241</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the truncated hemoglobin family. Group II subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A7I9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019825">
        <property type="term" value="F:oxygen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005344">
        <property type="term" value="F:oxygen carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19755">
        <property type="entry name" value="TrHb2_AtGlb3-like_O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.490.10">
        <property type="entry name" value="Globins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044203">
        <property type="entry name" value="GlbO/GLB3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009050">
        <property type="entry name" value="Globin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012292">
        <property type="entry name" value="Globin/Proto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001486">
        <property type="entry name" value="Hemoglobin_trunc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47366">
        <property type="entry name" value="TWO-ON-TWO HEMOGLOBIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47366:SF1">
        <property type="entry name" value="TWO-ON-TWO HEMOGLOBIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01152">
        <property type="entry name" value="Bac_globin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46458">
        <property type="entry name" value="Globin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0349">Heme</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0561">Oxygen transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9702408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="171" mass="19728" checksum="477C713BA28BE14A" modified="2024-01-24" version="1">MQSLQEKASEWSGVSQNDAFEIDNTNLFQKLGLQTFINLSTNFYTRVFDDEEEWFRLIFANSKKEEAIQNQYEFFVQRMGGPPLYSQRKGHPALIGRHRPFPVTHQAAERWLHHMQQALDSVTEIDADSKTKMMNFFRHTAFFLVAGDELKNRNQGVPCKHGASKPAASMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A7W7</accession>
    <name>A0AA39A7W7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mechanosensitive ion channel protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_004028</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the MscS (TC 1.A.23) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A7W7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050982">
        <property type="term" value="P:detection of mechanical stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006820">
        <property type="term" value="P:monoatomic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.60:FF:000003">
        <property type="entry name" value="Predicted mechanosensitive ion channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016688">
        <property type="entry name" value="MscS-like_plants/fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023408">
        <property type="entry name" value="MscS_beta-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31618:SF20">
        <property type="entry name" value="MECHANOSENSITIVE ION CHANNEL PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31618">
        <property type="entry name" value="MECHANOSENSITIVE ION CHANNEL PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017209">
        <property type="entry name" value="Memb_At2g17000_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0407">Ion channel</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="184"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="222"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="266"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="296"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="529"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="561"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="domain" description="Mechanosensitive ion channel MscS" evidence="12">
        <location>
            <begin position="580"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="23"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR017209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9702087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="759" mass="85636" checksum="4C2749FAAB70BC80" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AA07</accession>
    <name>A0AA39AA07_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">WRKY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_004381</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AA07"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050832">
        <property type="term" value="P:defense response to fungus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031347">
        <property type="term" value="P:regulation of defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002237">
        <property type="term" value="P:response to molecule of bacterial origin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009751">
        <property type="term" value="P:response to salicylic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.80:FF:000008">
        <property type="entry name" value="WRKY transcription factor 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.80">
        <property type="entry name" value="WRKY domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003657">
        <property type="entry name" value="WRKY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036576">
        <property type="entry name" value="WRKY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044810">
        <property type="entry name" value="WRKY_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31429">
        <property type="entry name" value="WRKY TRANSCRIPTION FACTOR 36-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31429:SF38">
        <property type="entry name" value="WRKY TRANSCRIPTION FACTOR 40-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03106">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00774">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118290">
        <property type="entry name" value="WRKY DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50811">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="WRKY" evidence="8">
        <location>
            <begin position="97"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="197"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="13"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="64"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9702613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="257" mass="28597" checksum="8604079625E4803F" modified="2024-01-24" version="1">MAAFDGGIYNHKVEVLKIELERLRKENEDLRLALEIMGSKYEVLHSRLQKNMATISPDLGSSHDSNKRPRTEEVPVAKASQVFVRTNPKDKSLTVKDGFQWRKYGQKITKDNPSPRAYFRCSMAPQCPVKKKVQRCMEDSSILVATYEGAHNHEPPHDAGGSSYSPDSPIKGLVANFPCPTTVDPFQPTVTLDLTLSGTGTGQEKRRPQNFMEDDQKSNCSRVEEYVASLTKDTNFTLALAAAVARSITDQPKPTKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AA87</accession>
    <name>A0AA39AA87_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_005043</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AA87"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9703534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="93" mass="10529" checksum="112D622EA9B21CA2" modified="2024-01-24" version="1">MESLFSKTCIRALKYKFINEAAGPVECWLSTLSHGDSNIYELGLLSGHAISHNLLVPAEHFLYHSITEVAYKQWLCHCSHQLSHIVPTIFMSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AAL7</accession>
    <name>A0AA39AAL7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Leucine-rich repeat extensin-like protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_005365</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9704046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AAL7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009834">
        <property type="term" value="P:plant-type secondary cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044950">
        <property type="entry name" value="TED6/7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35697">
        <property type="entry name" value="OS08G0108300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35697:SF1">
        <property type="entry name" value="PROTEIN TRACHEARY ELEMENT DIFFERENTIATION-RELATED 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="173"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="12"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="271"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="282"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9704046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="293" mass="31492" checksum="A0A23668182D38C8" modified="2024-01-24" version="1">MASVQNYGYFPYFPLPPPHSPSPPKVMPPHTSPPPAKVAPPHLPTTPPPPPPFPTPAPPTSPSPPKVAPPHLPTTPPPPPFPTPASPTSPSPPKVAPPHQPIRPPPPPSFPTPAPPPHHSFPPPPPAAKPPSHAIPPPAPIIKPPPHPHFPPPPPHIVPTPPPPPPPGHHSTVIIIVFVSLGGLFFLAFLSVALCCFIKKKKKKMVQETDIVKIDEHLKVHEAIIPGPHGPQVMILSIEDDVHIQEEIKKNEEIQGSHVRSTQDIPQALDMEASTSESSQHHIMHNHKQQSQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ABG2</accession>
    <name>A0AA39ABG2_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_002877</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O-methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ABG2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000836">
        <property type="entry name" value="O-methyltransferase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000294">
        <property type="entry name" value="O-methyltransferase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016461">
        <property type="entry name" value="COMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001077">
        <property type="entry name" value="COMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012967">
        <property type="entry name" value="COMT_dimerisation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11746">
        <property type="entry name" value="O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08100">
        <property type="entry name" value="Dimerisation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00891">
        <property type="entry name" value="Methyltransf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005739">
        <property type="entry name" value="O-mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51683">
        <property type="entry name" value="SAM_OMT_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="O-methyltransferase dimerisation" evidence="7">
        <location>
            <begin position="20"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="O-methyltransferase C-terminal" evidence="6">
        <location>
            <begin position="125"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR005739-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9704526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="353" mass="38508" checksum="6D74273A4A1FE14E" modified="2024-01-24" version="1">MEKVVKTMEKEEAEAEVEMWKYIFGFVEMAVVKCAIELGVADVIESHAGPITLSSLSSSLGCSPSGLYRIMRFLVNRRLFKEVATSQGDTGYQQTPLSRRLMTRSENGMAALLLLESSPVMLAPWHGLSARLLGKGNATFDAAHGQDVWGYAASHPAHSKLINDAMACDARMAVSAIVNGCPEVFDGVSTLVDVGGGDGTALRTLIKARPLIRGINFDLPHVVSAAPKCNGVEYVSGDMFDTVPKADAAFIMWVLHDWGDEECIQILEKCRQAIPGDKGKVIIVEAVIQENEKEGGNNLKDVGLMLDMVMMAHTTAGKERTLKEWDYVLKKAGFNRYTVKPIRAVKSVIEAYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AEB3</accession>
    <name>A0AA39AEB3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glycoside hydrolase family 5 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_001561</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AEB3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31263">
        <property type="entry name" value="CELLULASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_5G14560)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31263:SF0">
        <property type="entry name" value="CELLULASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_5G14560)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00150">
        <property type="entry name" value="Cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="Ricin B-like lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041406040" description="Glycoside hydrolase family 5 domain-containing protein" evidence="5">
        <location>
            <begin position="25"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="6">
        <location>
            <begin position="67"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9706050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="545" mass="61188" checksum="2F51ABCCFF335537" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AGI4</accession>
    <name>A0AA39AGI4_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_001530</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AGI4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9706003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="87" mass="9555" checksum="4ED45912CCB405C0" modified="2024-01-24" version="1">MRGTAALLPAKNKLKQIYPPKGIWGSVSTSTLKREGNNDFREQGTITNEKIDPIVAFSKPPPLPPVLGPLVALSLLEAWWSRDSNDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AGR4</accession>
    <name>A0AA39AGR4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phytocyanin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_001604</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AGR4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13920">
        <property type="entry name" value="Stellacyanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000034">
        <property type="entry name" value="Cupredoxin superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028871">
        <property type="entry name" value="BlueCu_1_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039391">
        <property type="entry name" value="Phytocyanin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003245">
        <property type="entry name" value="Phytocyanin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33021">
        <property type="entry name" value="BLUE COPPER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33021:SF264">
        <property type="entry name" value="OS05G0570900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02298">
        <property type="entry name" value="Cu_bind_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00196">
        <property type="entry name" value="COPPER_BLUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51485">
        <property type="entry name" value="PHYTOCYANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0186">Copper</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041236593" description="Phytocyanin domain-containing protein" evidence="5">
        <location>
            <begin position="23"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="Phytocyanin" evidence="6">
        <location>
            <begin position="23"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9706123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="171" mass="18287" checksum="47F6E1D65E6B6CD6" modified="2024-01-24" version="1">MHLISFLMLAAVTCFMTAPVAAFNHIVGGSFGWSTPGNLSFYEDWAKPRTFGVGDKLVFPFRTGVHSVVQVSEEEFQNCTQNDAIDMHYSGPLIIELPKTGTFYYYCGVGTHCEAGQKLKVTVVNAEGSAGTPITPNASVSAPADHKSSAKEGCDVGMVSGMLVLLLSVFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AH88</accession>
    <name>A0AA39AH88_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_002127</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AH88"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050317">
        <property type="entry name" value="Plant_Fungal_Acyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31642:SF299">
        <property type="entry name" value="OS02G0653400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31642">
        <property type="entry name" value="TRICHOTHECENE 3-O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9707007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="372" mass="41274" checksum="2A6E9C482570043B" modified="2024-01-24" version="1">MAGEGSSSSTAATSVTMSRIEAVQSVMPAKVTDPRLSRKISVRDPLGCGIFGRRLHTVLYFKSVTKEDSGWLVAGWIRESLGRALVEQPLLGGRLRRVGDSEGEFEIVSNDSGVRLMEARTSMALSEFLELKDWEAVEADQLVFWKDIDEQNPQFYPLFYVQVTNFECGGYSIGISCSLLLADPLGMASFLKRWASIHNTLVSENDMSKLPIFYLLSLRRLNDTPSIHMISSNTSINRARAKIFNTAENMNSDDKICRTVASVCIEEAECHLGTKMASKYWLFLKKLSEDIKIEACSKEEIVPLSISGLSELRWDDSGVHEVAFFQGNRPIHVSFWIGSLINDGLVIIAPSSSDEGCSGKNVVVITPEEEEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AI61</accession>
    <name>A0AA39AI61_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C-JID domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_002492</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AI61"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045344">
        <property type="entry name" value="C-JID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011713">
        <property type="entry name" value="Leu-rich_rpt_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017:SF479">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN (TIR-NBS-LRR CLASS) FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20160">
        <property type="entry name" value="C-JID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07725">
        <property type="entry name" value="LRR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="C-JID" evidence="4">
        <location>
            <begin position="330"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="468"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="483"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF20160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9707480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="507" mass="57839" checksum="F1A227F09D7A2938" modified="2024-01-24" version="1">MKWLRLLKVYKSNFLMDFKREECKVHFSRGFKFHCEELRHLYWYEYPLKSLPNDFNLKNLVDLNMPYNQIKQLWKETKVLENLKFMNLKHSKFLTETLDFSRVTNLEQLVLKGCISLYKVHPLLGDSNKLNFLSLKNCNMLKSLPNSICDLKCLEIFILLNFGNLEMLKEFYADRTAIRVLPSSFSLLRNLEILSFKGCKGPPPSTSWLLLRSSNFHGLVWSPLLSFGSLKTLRLHACNISDEATLDSLGFLYSLEDLDLSENILVTLPSNISQLSHLKMLGPTNYTSLETISNQSFGSLLMTIKLKEHIYCPINRDGLLLPALSTVIFGSRIPDWIRYQSSGTEVKADLPPNWFNSNFLGLVSLADFSGLFWRSCTLFYSTSNHASSSLDVESDHMWLVYVPLPHFINWHQVTHIKASFRITTFMCLSAIKECGIGLVYVNEEVNYSVFSPPPPPPNESSVVLQEIHEEEPSGSGYSNIDGSESENSDYYSADEGEPTAPLLLGSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AJY8</accession>
    <name>A0AA39AJY8_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_002589</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AJY8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36034">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36034:SF2">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="374"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="374"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="385"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9707624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="854" mass="92819" checksum="2C269DC107D34D49" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D585</accession>
    <name>A0AA39D585_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Aminotransferase-like plant mobile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_027255</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9671184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D585"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34835">
        <property type="entry name" value="OS07G0283600 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="165"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="233"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9671184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="396" mass="45504" checksum="3158DDEB9444F5C3" modified="2024-01-24" version="1">MGIPHTGRKLIVDRGTVNASQMPSLQDIEAMMVDIDDIEEFKRLFLIFECATLLAPTSRLEGSHSLWYTPREQLLGNINWGEFVLEFLIQAIHEHRRKESVWIKGCLMFLQIFYFSISNFPVMYVELTTPRIAAWNDILVKQRIKLELEMLGGFGKVELVSISTEQDETEKNKAPAVDDPVDPDETEDTDVIYARMEATQRQITDAQSHLNSLIASYNRDMKVLRTRFTSSHYRTDEGQPSNFHEHINESGARFDTNNPQHSPVHSCGEGRSFEDDVGCTNEGVSERPNVEEGVFFTNEDHFHPGPHNMLVLVQSSMENNADVTPQAPEEIRPRRVKMKARHHRQRAAACKSPFVQFCVSKYKRLTEEETHVADYVFDESKDPRLVPNHKFSKYCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D5L7</accession>
    <name>A0AA39D5L7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_026152</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the RING-type zinc finger family. ATL subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D5L7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046948">
        <property type="entry name" value="ATL20-22-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025287">
        <property type="entry name" value="WAK_GUB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46279:SF9">
        <property type="entry name" value="OS01G0116300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46279">
        <property type="entry name" value="RING/U-BOX SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13947">
        <property type="entry name" value="GUB_WAK_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 15" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041445288" description="RING-type E3 ubiquitin transferase" evidence="15">
        <location>
            <begin position="23"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="Wall-associated receptor kinase galacturonan-binding" evidence="16">
        <location>
            <begin position="24"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00024209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF13947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9672803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="233" mass="26488" checksum="97BE5C752C8B04DA" modified="2024-01-24" version="1">MDRYLCLFLFLFVTLFVEMGGGRDECMPHHCGYPGFELSCSENNKTMLDLPVSVKLLVKKIAYKSQEIIVQDPDNCLARQLRNLNLAASPFHFKFEWDFTFFTCSSDKIELSRFLSIPCLSIPGNLLVIIGKYCVFYAVYSSGPVQYTDLSSCRRLYNWSKSICGSCTRGGKICRLKKSNSTEPENECIKVKGYECGAKILDGIYILKLLLDEFLAKVIYCFSCISPQNIELN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D5S6</accession>
    <name>A0AA39D5S6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Disease resistance R13L4/SHOC-2-like LRR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_027123</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Leucine-rich repeat protein that likely mediates protein interactions, possibly in the context of signal transduction.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SHOC2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D5S6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000405">
        <property type="entry name" value="Plant intracellular Ras-group-related LRR protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF54">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="6">
        <location>
            <begin position="318"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="116"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00037519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9670990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="557" mass="62077" checksum="A32F4DC4CA3FD832" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D678</accession>
    <name>A0AA39D678_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">S-protein homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_025994</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the plant self-incompatibility (S1) protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D678"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060320">
        <property type="term" value="P:rejection of self pollen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010264">
        <property type="entry name" value="Self-incomp_S1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31232">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31232:SF149">
        <property type="entry name" value="S-PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05938">
        <property type="entry name" value="Self-incomp_S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 6" id="KW-0713">Self-incompatibility</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="158"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="163"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9672596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="185" mass="21770" checksum="378F8EF34DE90B83" modified="2024-01-24" version="1">MKETKHQFTPAKKITTMFPFSRCLFSVVLFVFLLRLCDGAFIEKKVHLRITNDLGNGLDLNLHCKSQDDDLGAHVLAPHQFFEFSFRPNFWATTLYFCRFWWGGESHWFDIYVQNRDIERCNKKCWWMIDPTGPCLLNDKVKRYTYCENWNDQQIQGSSQIGTGNSTARMSNDSEYLEGNEKRGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D683</accession>
    <name>A0AA39D683_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Alpha-1,4 glucan phosphorylase</fullName>
            <ecNumber evidence="2">2.4.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_026008</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Allosteric enzyme that catalyzes the rate-limiting step in glycogen catabolism, the phosphorolytic cleavage of glycogen to produce glucose-1-phosphate, and plays a central role in maintaining cellular and organismal glucose homeostasis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>[(1-&gt;4)-alpha-D-glucosyl](n) + phosphate = [(1-&gt;4)-alpha-D-glucosyl](n-1) + alpha-D-glucose 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:41732"/>
            <dbReference type="Rhea" id="RHEA-COMP:9584"/>
            <dbReference type="Rhea" id="RHEA-COMP:9586"/>
            <dbReference type="ChEBI" id="CHEBI:15444"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="EC" id="2.4.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the glycogen phosphorylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.1" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D683"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008184">
        <property type="term" value="F:glycogen phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005980">
        <property type="term" value="P:glycogen catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000811">
        <property type="entry name" value="Glyco_trans_35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11468:SF4">
        <property type="entry name" value="ALPHA-GLUCAN PHOSPHORYLASE 2, CYTOSOLIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11468">
        <property type="entry name" value="GLYCOGEN PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00343">
        <property type="entry name" value="Phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9672611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="90" mass="10424" checksum="FCA746764EFA2D11" modified="2024-01-24" version="1">MSLSFKSWINDSGVNCSFHSRARQICAVLYPRDATESGKLLRLKQQFFLCNASLQNIIFRFKERKDGGSWQWSEFPSEVVVQLNDNTSYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D6H3</accession>
    <name>A0AA39D6H3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Receptor-like serine/threonine-protein kinase</fullName>
            <ecNumber evidence="17">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">PVL29_025505</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="24">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="17"/>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D6H3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048544">
        <property type="term" value="P:recognition of pollen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00028">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01098">
        <property type="entry name" value="PAN_AP_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000060">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.90.10.10:FF:000004">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000195">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.4.10:FF:000002">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.10">
        <property type="entry name" value="Bulb-type lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.4.10">
        <property type="entry name" value="Hepatocyte Growth Factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021820">
        <property type="entry name" value="S-locus_recpt_kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000858">
        <property type="entry name" value="S_locus_glycoprot_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024171">
        <property type="entry name" value="SRK-like_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32444:SF183">
        <property type="entry name" value="APPLE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32444">
        <property type="entry name" value="BULB-TYPE LECTIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11883">
        <property type="entry name" value="DUF3403"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08276">
        <property type="entry name" value="PAN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00954">
        <property type="entry name" value="S_locus_glycop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000641">
        <property type="entry name" value="SRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00473">
        <property type="entry name" value="PAN_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 17 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 17 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 17" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 20" id="KW-0732">Signal</keyword>
    <keyword evidence="4 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041455125" description="Receptor-like serine/threonine-protein kinase" evidence="20">
        <location>
            <begin position="21"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="434"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="22">
        <location>
            <begin position="21"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="23">
        <location>
            <begin position="336"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="497"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="525"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRNR" id="PIRNR000641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KAJ9671830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="811" mass="90671" checksum="5C936C4017145046" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D6Z9</accession>
    <name>A0AA39D6Z9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Retrotransposon Copia-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_026323</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D6Z9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029472">
        <property type="entry name" value="Copia-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37610">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37610:SF97">
        <property type="entry name" value="RETROTRANSPOSON GAG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14244">
        <property type="entry name" value="Retrotran_gag_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Retrotransposon Copia-like N-terminal" evidence="2">
        <location>
            <begin position="40"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9672999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="134" mass="15090" checksum="9A7586ABB48A4F04" modified="2024-01-24" version="1">MARRGSTTSSSNRFGTLDSTHSDQSQMDDYESPYFLDNGDHPTVSLVSHHLTGGNYNTWNRVMSMALNAKNKLGFVDGIISCPCATDLLFGTWSLCNSMVISWILNVVSKEIADNLLYIDTIVEVWRDLHDRFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D9X9</accession>
    <name>A0AA39D9X9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Alkaline/neutral invertase</fullName>
            <ecNumber evidence="6">3.2.1.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_024513</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Invertase that cleaves sucrose into glucose and fructose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.</text>
            <dbReference type="EC" id="3.2.1.26"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the glycosyl hydrolase 100 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.26" evidence="6"/>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D9X9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033926">
        <property type="term" value="F:endo-alpha-N-acetylgalactosaminidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004575">
        <property type="term" value="F:sucrose alpha-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005987">
        <property type="term" value="P:sucrose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024746">
        <property type="entry name" value="Glyco_hydro_100"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31916">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31916:SF15">
        <property type="entry name" value="ALKALINE_NEUTRAL INVERTASE D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12899">
        <property type="entry name" value="Glyco_hydro_100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5 6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9675625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="433" mass="49787" checksum="D165BA20CE94634B" modified="2024-01-24" version="1">MEGTKESGLKNVGSHCSISEMDDYDLSRVLEKPRLNIERQRSFDERSMSELSIGLARHLEHLDSMYSPGGRSGFDTPASSARNSFEPHPMVNEAWEALRRSLVFFRGQPVGTIAAYDHASEEVLNYDQGFDTFPTLLCADGCSMVDRRMGIYGYPIEIQALFFMALRCALAMLKQDAEGKECIERIVKRLHALSYHMRSYFWLDFQQLNDIYRYKTEEYSHTAVNKFNVIPDSIPDWVFDFMPTRGGYFIGNVSPARMDFRWFALGNCVAILSSLATPEQSMAIMDLIESRWEELVGEMPLKISYPTFENHEWRIITGCDPKNTRWSYHNGGSWPVLLWLLTAACIKTGRPQIARRAIDLAESRLLKDSWPEYYDGKLGRYVGKQARKFQTWSIAGYLVAKMMLEDPSHLGMISLEEDRQMKPLIKRSSSWTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DA66</accession>
    <name>A0AA39DA66_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase</fullName>
            <ecNumber evidence="3">3.2.2.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_025064</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>NAD(+) + H2O = ADP-D-ribose + nicotinamide + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16301"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57967"/>
            <dbReference type="EC" id="3.2.2.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:16302"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the disease resistance TIR-NB-LRR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.6" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9676359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061809">
        <property type="term" value="F:NAD+ nucleosidase activity, cyclic ADP-ribose generating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050832">
        <property type="term" value="P:defense response to fungus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043068">
        <property type="term" value="P:positive regulation of programmed cell death"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10140:FF:000007">
        <property type="entry name" value="Disease resistance protein (TIR-NBS-LRR class)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045344">
        <property type="entry name" value="C-JID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058192">
        <property type="entry name" value="WHD_ROQ1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017:SF570">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN (TIR-NBS CLASS)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20160">
        <property type="entry name" value="C-JID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23282">
        <property type="entry name" value="WHD_ROQ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0611">Plant defense</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="TIR" evidence="14">
        <location>
            <begin position="34"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1546"/>
            <end position="1571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1546"/>
            <end position="1563"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00061488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9676359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1571" mass="177328" checksum="0994ACD2C0C25789" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DAM2</accession>
    <name>A0AA39DAM2_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_024295</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DAM2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32108">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32108:SF9">
        <property type="entry name" value="REVERSE TRANSCRIPTASE RNASE H-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9675277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="638" mass="71452" checksum="EAF3423C2EA0332B" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DAZ4</accession>
    <name>A0AA39DAZ4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_022464</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DAZ4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057670">
        <property type="entry name" value="SH3_retrovirus"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439">
        <property type="entry name" value="GAG-POL-RELATED RETROTRANSPOSON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439:SF467">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25597">
        <property type="entry name" value="SH3_retrovirus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="2">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9677486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="789" mass="88426" checksum="0871A9202EF9B82A" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DC96</accession>
    <name>A0AA39DC96_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_021579</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the major facilitator superfamily. Phosphate:H(+) symporter (TC 2.A.1.9) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DC96"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17364">
        <property type="entry name" value="MFS_PhT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000175">
        <property type="entry name" value="Inorganic phosphate transporter 1-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0769">Symport</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="21"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="124"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="163"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="212"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="344"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="370"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="400"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="439"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="471"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="11">
        <location>
            <begin position="23"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="498"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="508"/>
            <end position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9679703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="517" mass="57608" checksum="8308788590E00198" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DDG9</accession>
    <name>A0AA39DDG9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Plastid division protein PDV1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_021580</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DDG9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010020">
        <property type="term" value="P:chloroplast fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038939">
        <property type="entry name" value="PDV1/PDV2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33600:SF4">
        <property type="entry name" value="PLASTID DIVISION PROTEIN PDV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33600">
        <property type="entry name" value="PLASTID DIVISION PROTEIN PDV2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="201"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9679704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="272" mass="30842" checksum="1E21F35AAAAF7483" modified="2024-01-24" version="1">MEIEEIEAILEKIWDLHDKLSDAIHSISRVHFLNSIKSLRKSDKSKPLNDVDENRFGFVYAKDFRVEDDDLAIQEAKSLNAIRTALENLEDQLEFFHTVQVHQQAERDAALARLEQSRIVLAIRLSEHHGKKYKVIEEALAFVGDVRDAGPFVLPDNLFGPPVSPPGKNVAPHEEKRSNFLIKVLISCFNFGKKSLKLDHMGGILGNAALVAVSMLALLHLNQVTYKDKIVLEPPQKREDNIYRNQNVKRTSRVESSLSSELNQLDVLYARG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DHC2</accession>
    <name>A0AA39DHC2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Scaffold protein Nfu/NifU N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_017073</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Molecular scaffold for [Fe-S] cluster assembly of mitochondrial iron-sulfur proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NifU family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DHC2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.130:FF:000001">
        <property type="entry name" value="NFU1 iron-sulfur cluster scaffold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.70:FF:000001">
        <property type="entry name" value="NifU-like protein 4, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.130">
        <property type="entry name" value="Fe-S cluster assembly (FSCA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.70">
        <property type="entry name" value="Scaffold protein Nfu/NifU, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014824">
        <property type="entry name" value="Nfu/NifU_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036498">
        <property type="entry name" value="Nfu/NifU_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001075">
        <property type="entry name" value="NIF_FeS_clus_asmbl_NifU_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11178">
        <property type="entry name" value="IRON-SULFUR CLUSTER SCAFFOLD PROTEIN NFU-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11178:SF1">
        <property type="entry name" value="NFU1 IRON-SULFUR CLUSTER SCAFFOLD HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08712">
        <property type="entry name" value="Nfu_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01106">
        <property type="entry name" value="NifU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00932">
        <property type="entry name" value="Nfu_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="Fe-S cluster assembly (FSCA) domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110836">
        <property type="entry name" value="Hypothetical protein SAV1430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Scaffold protein Nfu/NifU N-terminal" evidence="3">
        <location>
            <begin position="72"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9684908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="271" mass="29951" checksum="DCB75F462219F813" modified="2024-01-24" version="1">MKGLGRLIGRRLLCKRLEPWRKPLMPRSLIFSSFTTTSYLENPTSSSRFTSFKSSSLPKWSHFGGQRRTMFIQTQSTPNPASLMFYPGKPVMEVGSADFPNSRSAMSSPLAKSLYGIDGITRVFFGSDFITVTKSDDASWDFIKPEIFAAIMDFYSSGKPLFLDSNTAAAMDTAIHEDDSETVAMIKELLETRIRPAVQDDGGDIEYRGFDPETGIVKLKMQGACSGCPSSSVTLKSGIENMLMHYVPEVKGVEQELDAEDEDVALSGQMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DIY6</accession>
    <name>A0AA39DIY6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Endoplasmic reticulum-Golgi intermediate compartment protein 3-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_014998</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ERGIC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9685901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DIY6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045888">
        <property type="entry name" value="Erv"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012936">
        <property type="entry name" value="Erv_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039542">
        <property type="entry name" value="Erv_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10984">
        <property type="entry name" value="ENDOPLASMIC RETICULUM-GOLGI INTERMEDIATE COMPARTMENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10984:SF25">
        <property type="entry name" value="ENDOPLASMIC RETICULUM-GOLGI INTERMEDIATE COMPARTMENT PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07970">
        <property type="entry name" value="COPIIcoated_ERV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13850">
        <property type="entry name" value="ERGIC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="312"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="Endoplasmic reticulum vesicle transporter N-terminal" evidence="8">
        <location>
            <begin position="7"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="Endoplasmic reticulum vesicle transporter C-terminal" evidence="7">
        <location>
            <begin position="151"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9685901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="351" mass="40143" checksum="0E523D19E4E1BEB9" modified="2024-01-24" version="1">MGVKQFIKSLHAFPRAEEHLLQKTQSGAVVSIIGLVIMATLFLHELRYYLTTYTVHQMSVDLKRGETLPIHINMTFPSLPCDVLSVDAIDMSGKHEVDLDTNIWKLRLNRDGFIIGTEYLSDLVEKEHADHKHDHNKDHHGDSDQKLHAHSFDQDAENMVKKVKQALANGEGCRVYGVLDVQRVAGNFHISVHGLNIFVAQLIFDGAIHVNVSHIIHDLSFGPKYPGLHNPLDGTVRILHGASGTFKYYIKIVPTEYRYISKEVLPTNQFSVMEYFSPMNEFDRTWPAVYFLYDLSPVTVTIKEERRSFLHFITRLCAVLGGTFALTGMLDRWMYRFLEMLTKPNAKSVYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DJ27</accession>
    <name>A0AA39DJ27_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_017761</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DJ27"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041467415" evidence="1">
        <location>
            <begin position="27"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9685824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="69" mass="7349" checksum="CEB7CABDB96E5A9E" modified="2024-01-24" version="1">MVIQGSLFLTLLIVVSVLAWTEPVVATSLNRINFPADFAFGTASSSYQDGKGPSITDTFTHKYPGLSLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DK82</accession>
    <name>A0AA39DK82_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_015466</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DK82"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9686605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="126" mass="13967" checksum="0CDC778975EF6321" modified="2024-01-24" version="1">MAGTSVFDHGFKGYQILDRTETGGEENGSWRTLSRRKKGPYNMVTSLSYRQERAKKRQIFLQSYKLSTIDSTLGGSKNSCKLKRVMIKVKAVVVSIVSISRIGSWKSCNSRSAICASSPTSTAKCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DLC7</accession>
    <name>A0AA39DLC7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Translation initiation factor IF-3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_014602</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Chloroplast translation initiation factor that is essential for the coordination of leaf and chloroplast development. IF-3 binds to the 30S ribosomal subunit and shifts the equilibrium between 70S ribosomes and their 50S and 30S subunits in favor of the free subunits, thus enhancing the availability of 30S subunits on which protein synthesis initiation begins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Plastid</location>
            <location evidence="1 9">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the IF-3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9689033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DLC7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032790">
        <property type="term" value="P:ribosome disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.80:FF:000003">
        <property type="entry name" value="Translation initiation factor IF-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.110.10:FF:000003">
        <property type="entry name" value="Translation initiation factor IF-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.10">
        <property type="entry name" value="Translation initiation factor 3 (IF-3), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.80">
        <property type="entry name" value="Translation initiation factor 3 (IF-3), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036788">
        <property type="entry name" value="T_IF-3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036787">
        <property type="entry name" value="T_IF-3_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019813">
        <property type="entry name" value="Translation_initiation_fac3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001288">
        <property type="entry name" value="Translation_initiation_fac_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019815">
        <property type="entry name" value="Translation_initiation_fac_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019814">
        <property type="entry name" value="Translation_initiation_fac_3_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00168">
        <property type="entry name" value="infC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10938">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR IF-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10938:SF0">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR IF-3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00707">
        <property type="entry name" value="IF3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05198">
        <property type="entry name" value="IF3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55200">
        <property type="entry name" value="Translation initiation factor IF3, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54364">
        <property type="entry name" value="Translation initiation factor IF3, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00938">
        <property type="entry name" value="IF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="4 9" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0934">Plastid</keyword>
    <keyword evidence="6 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Translation initiation factor 3 N-terminal" evidence="12">
        <location>
            <begin position="88"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="Translation initiation factor 3 C-terminal" evidence="11">
        <location>
            <begin position="163"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="61"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="253"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="255"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00057772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF05198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9689033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="272" mass="30686" checksum="C2EE17963C4F6A4A" modified="2024-01-24" version="1">MAGLTSGIPFKPFALRTKTTSSPLYFSLDSQLFGVRLCSPTFSKCRSFSSSLHRSVTARYGGYRSSGDSWRSRQRDSDDDQALDVSSVRSASVRLIDEQQNMVGVVSKSEAIQMAEDAELDLVVLSPDADPPVLRIMDYNKYRYELQKKKRGQQKKSAANRMDLKELKMGYNIDSHDYSVRLKAARKFLQDGDKVKIIVNLKGRENEFRNIAIELIRRFQNDVGELATEESKNFSVRNIFIVLVPNKALLQKGQESSKKREKSPATEISAGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DMT0</accession>
    <name>A0AA39DMT0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Dof zinc finger protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_012907</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Transcription factor that binds specifically to a 5'-AA[AG]G-3' consensus core sequence.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DMT0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045174">
        <property type="entry name" value="Dof"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003851">
        <property type="entry name" value="Znf_Dof"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31992:SF108">
        <property type="entry name" value="DOF ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31992">
        <property type="entry name" value="DOF ZINC FINGER PROTEIN DOF1.4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02701">
        <property type="entry name" value="Zn_ribbon_Dof"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01361">
        <property type="entry name" value="ZF_DOF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50884">
        <property type="entry name" value="ZF_DOF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3 9" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 8 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Dof-type" evidence="11">
        <location>
            <begin position="36"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="77"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="237"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="26"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="102"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="244"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="265"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU369094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9690488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="300" mass="32732" checksum="0191307DAE33E20D" modified="2024-01-24" version="1">MQQDRDIGGGGSEDVDPPDRRLKPAQSENQQQQLPQKCPRCDSINTKFCYYNNYSLSQPRYFCKTCRRYWTQGGTLRNVPVGGGCRKTKRKPSASGDNSRSQLPTQQQQQNLAAPPPNIISTNSGVAVGAALRITEPANLSPAPAPPPAPTLSSVNPYYPAGAFLSSLPAMRPLNQPQPQPFNQPVNVGRDFGGSNLALLQGFNVPSLGSQQPQRQQAEFYQMGNRDRNIESLYGSGEDLIQPSRPTGHQNNWHQTFINPTAPDSNLWSIGGSSNSNSNPTSSSFNQNHWADLQGYGPPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DND3</accession>
    <name>A0AA39DND3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_014173</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DND3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000373">
        <property type="term" value="P:Group II intron splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.110.60:FF:000002">
        <property type="entry name" value="CRS2-associated factor 1, chloroplastic"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.60">
        <property type="entry name" value="YhbY-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044599">
        <property type="entry name" value="CAF1P_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001890">
        <property type="entry name" value="RNA-binding_CRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035920">
        <property type="entry name" value="YhbY-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46247">
        <property type="entry name" value="CRS2-ASSOCIATED FACTOR 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46247:SF1">
        <property type="entry name" value="CRS2-ASSOCIATED FACTOR 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01985">
        <property type="entry name" value="CRS1_YhbY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01103">
        <property type="entry name" value="CRS1_YhbY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75471">
        <property type="entry name" value="YhbY-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51295">
        <property type="entry name" value="CRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="CRM" evidence="9">
        <location>
            <begin position="242"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="CRM" evidence="9">
        <location>
            <begin position="360"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="69"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="202"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="460"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="80"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9688342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="753" mass="83465" checksum="54DC31B3130EA1A2" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DP30</accession>
    <name>A0AA39DP30_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Spermidine hydroxycinnamoyl transferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_014609</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9689047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DP30"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000008">
        <property type="entry name" value="Tryptamine hydroxycinnamoyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050317">
        <property type="entry name" value="Plant_Fungal_Acyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31642:SF324">
        <property type="entry name" value="SPERMIDINE HYDROXYCINNAMOYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31642">
        <property type="entry name" value="TRICHOTHECENE 3-O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="221"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9689047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="457" mass="51022" checksum="2AF3452AF3FCF11D" modified="2024-01-24" version="1">MVKLMATYTVRPAKETPGGYRCLSDCDQARTINHAPIIYFYPPVNMSLESATEILRHSLSEALVIFYPLAGRLHWIGGGRLVLECNALGALLIAVESDAKIDDFGDFRPTQEIQALIPSVDYNKPIHELPLLLVQVTKFSCGGISLGLGTSRIMTDGLSAFLFIQEWAKIARGEQPNCPPFLDRSILLAPENLTAPVFDHPAYSPQPLLIGNQDHMEERKRESATLTLKLSKDQVEKLKDKANKDRSAFNKNRPYTRYEAVAGHIWRTACKARQHESQQPTTLSIIVDARNRMEPPLPPRYFGSSIFRVTAESTSGELVSKPLGYASGKARQAIEKATHEYLQSALVYVKCQEDVTQFRNFHTVGYAKGDFYGNPNLKITSWAGLPIYGADFGWGQEIYMGPGVIRNDGKVFVLPSRGQDGSFLVVLGLQVRHIDAFQKFFYEDICLQKPLVINPSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DSA2</accession>
    <name>A0AA39DSA2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_009664</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9693805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DSA2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23127">
        <property type="entry name" value="RING-HC_BAH1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14482">
        <property type="entry name" value="SPX_BAH1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033326">
        <property type="entry name" value="BAH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46764">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE BAH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46764:SF2">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE BAH1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SPX" evidence="11">
        <location>
            <begin position="1"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="228"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9693805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="332" mass="38370" checksum="7D9EF044C67DF5A0" modified="2024-01-24" version="1">MKFGETFMEYLHGEQESFLDKFSHVEYKRLKKVLKTCRSLHGSYVADEQEDENGDALSQLCRYESCPLCDKMFFSELMKEASDIAGCFSSRVRHLLHLHIATGMQRYVLLLRQCFKNDRQTIAEEGRMLIDYVTMNAIAIRKILKKYDKVHSSVNGRDFKRKMQAKHIELLQSPWLIELGAFYLNFNESDEEESCKISCQFSCDLSTTEPVMTLRLPDSMKLECNLTCPICLDTLFIPHALSCGHLFCKSCACSAASVMIFQGLKAASPESKCPICREAGVYSNAVEMLELGLLLKRRCREYWKERLIAERAEMVKQSKDYWDSQTRYMVGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DU09</accession>
    <name>A0AA39DU09_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_008919</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DU09"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000001">
        <property type="entry name" value="MYB-related transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015495">
        <property type="entry name" value="Myb_TF_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10641">
        <property type="entry name" value="MYB FAMILY TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10641:SF1346">
        <property type="entry name" value="TRANSCRIPTION FACTOR MYB14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="HTH myb-type" evidence="7">
        <location>
            <begin position="9"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="6">
        <location>
            <begin position="9"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="7">
        <location>
            <begin position="62"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="6">
        <location>
            <begin position="62"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="115"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="128"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="138"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9696923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="268" mass="30132" checksum="C65D98C82CC813EF" modified="2024-01-24" version="1">MGRAPCCEKMGLKKGPWTPEEDQILINYIHLYGHGNWRALPKQAGLLRCGKSCRLRWTNYLRPDIKRGNFTIEEEETIIDLHERLGNRWSAIAAKLPGRTDNEIKNVWHTHLKKRLKHNHATPPPKRHSLDASHIEKQQNPIDSATNSRSESLGYGPVLSPQQSFSDISSAATTTTTATMSDTTTPCIKVDSLEDFPEMDENFWSEVLSSNKSGAAGDFPAAASGPQLQFPLSPRAVFGSSPYSTYDMDEFWYNIFTRSGELHELSEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DU32</accession>
    <name>A0AA39DU32_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_009433</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DU32"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006315">
        <property type="term" value="P:homing of group II introns"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090615">
        <property type="term" value="P:mitochondrial mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01651">
        <property type="entry name" value="RT_G2_intron"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024937">
        <property type="entry name" value="Domain_X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33642">
        <property type="entry name" value="COX1/OXI3 INTRON 1 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33642:SF3">
        <property type="entry name" value="NUCLEAR INTRON MATURASE 4, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01348">
        <property type="entry name" value="Intron_maturas2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="178"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="Domain X" evidence="2">
        <location>
            <begin position="580"/>
            <end position="681"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9697601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="801" mass="91124" checksum="6A31CFD81DF6BEDB" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DUD6</accession>
    <name>A0AA39DUD6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tetrapyrrole biosynthesis uroporphyrinogen III synthase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_011076</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DUD6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004852">
        <property type="term" value="F:uroporphyrinogen-III synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033014">
        <property type="term" value="P:tetrapyrrole biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06578">
        <property type="entry name" value="HemD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10090">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036108">
        <property type="entry name" value="4pyrrol_syn_uPrphyn_synt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003754">
        <property type="entry name" value="4pyrrol_synth_uPrphyn_synth"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38020">
        <property type="entry name" value="UROPORPHYRINOGEN-III SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38020:SF1">
        <property type="entry name" value="UROPORPHYRINOGEN-III SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02602">
        <property type="entry name" value="HEM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69618">
        <property type="entry name" value="HemD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tetrapyrrole biosynthesis uroporphyrinogen III synthase" evidence="1">
        <location>
            <begin position="37"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9695935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="311" mass="34085" checksum="3108B14F25784B34" modified="2024-01-24" version="1">MMNMAVTMQELSPIPPLLSNKKRAVAFTTPLNYAGRLSHLLQLKGWSPLCCPTIVVEPSPQTKASVQFFLSTKAQSNDNKKTPLEDFSAIAFTSRAGILVFSETLMRNEETPLEPYGDNFTVSALGKDAELLNEDFLSKLCKKPQRIRVTVPPVATPTGLVESLGVGRGRRVLCPVPLVAGLVEPPVVPNFLRDLALRDWVPVKVHAYETRWAGPRCAERVVRRSEGEEGLDAIVFTSTGEVEGLLKSLRELGLDWRMLKERYPGLVVAAHGPVTASGAERLGIRVDVVGSRFHSFDGVVDALALRWSASE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DWI4</accession>
    <name>A0AA39DWI4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Epidermal patterning factor-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_005975</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Controls stomatal patterning.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the plant cysteine rich small secretory peptide family. Epidermal patterning factor subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DWI4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010052">
        <property type="term" value="P:guard cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039455">
        <property type="entry name" value="EPFL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33109">
        <property type="entry name" value="EPIDERMAL PATTERNING FACTOR-LIKE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33109:SF55">
        <property type="entry name" value="EPIDERMAL PATTERNING FACTOR-LIKE PROTEIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17181">
        <property type="entry name" value="EPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041486437" description="Epidermal patterning factor-like protein" evidence="7">
        <location>
            <begin position="31"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="61"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9700436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="145" mass="16246" checksum="71676A48E758E2F6" modified="2024-01-24" version="1">MGVVRRRRHYHRHHRLLTALAFLLFASASAVNLAPHRHRGKNSKTHQTHLTQSRFFPHGFPGTGGLVGQEREERKRGSGSVALERVLTQKRLGGPGSSPPTCRSKCGRCTPCKAVHVPIQPGFSIPLEYYPEAWRCKCGNKLFMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DYT9</accession>
    <name>A0AA39DYT9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">chlorophyllase</fullName>
            <ecNumber evidence="4">3.1.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_005974</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a chlorophyll + H2O = a chlorophyllide + phytol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17327"/>
            <dbReference type="ChEBI" id="CHEBI:139291"/>
            <dbReference type="ChEBI" id="CHEBI:139292"/>
            <dbReference type="EC" id="3.1.1.14"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:19606"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Porphyrin-containing compound metabolism; chlorophyll degradation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.14" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DYT9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047746">
        <property type="term" value="F:chlorophyllase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015996">
        <property type="term" value="P:chlorophyll catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000159">
        <property type="entry name" value="Chlorophyllase-2, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048264">
        <property type="entry name" value="Chlorophyllase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017395">
        <property type="entry name" value="Chlorophyllase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33428:SF2">
        <property type="entry name" value="CHLOROPHYLLASE-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33428">
        <property type="entry name" value="CHLOROPHYLLASE-2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07224">
        <property type="entry name" value="Chlorophyllase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038128">
        <property type="entry name" value="Chlorophyllase_chloroplast"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0881">Chlorophyll catabolism</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="9">
        <location>
            <position position="146"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="9">
        <location>
            <position position="173"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="9">
        <location>
            <position position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00053022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR038128-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9700435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="322" mass="34701" checksum="8002F46B970867D6" modified="2024-01-24" version="1">MASHLTGVMSSSTCSTTATNVFEIGKHITVLLRAEPGTCTNKSSLPVPPPVQLLIATPSEAGEFPLLLLLHGYLLYNSFYSQLIQHIASHGFIVLAPQLYTVAGPDSSEEIKSAAALTNWLSKGLHDLLPPHVRPNLSKLGLAGHSRGGKTAFALALRKASTSLKFSALIGIDPVDGMDKGKQTPPPVLTYVPHSFDLDMAVLVIGSGLGEVKRNPLFPPCAPKGVNHEDFFKECREPACYFLAKDYGHFDMLDDETNGIRGKATHCLCKNGKSREPMRRFVGGIVVAFMKAYLAGDNSSLISIRDGHATPPVELQNVEFLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DZX7</accession>
    <name>A0AA39DZX7_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_004664</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DZX7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9702998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="229" mass="25418" checksum="E0D5ACFE16098041" modified="2024-01-24" version="1">MKIVGSQERPRGRSFLDHVAPSCIWPNQYAQSWIASPADEKDNWKSHGPHQQGCGGLCPGELVVEEVVAGHESIDEVVDLEKCIDTGLSHGNDPLSLNFYYGDCNKYHEDSKEFNENEQKPLLDMEENHCDPSGRGMFNVYLSYLDVDDENLQQMAFDSSKMMESENPLSNLELRKCLSVVSSFGDSGTSSSKKNPKASYQLLGDILALFASTSDAPTKDVAFRLNLAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E024</accession>
    <name>A0AA39E024_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Flavin-containing monooxygenase</fullName>
            <ecNumber evidence="6">1.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_002881</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the FMO family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.-.-.-" evidence="6"/>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E024"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004499">
        <property type="term" value="F:N,N-dimethylaniline monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000169">
        <property type="entry name" value="Flavin-containing monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000403">
        <property type="entry name" value="Flavin-containing monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000440">
        <property type="entry name" value="Flavin-containing monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000960">
        <property type="entry name" value="Flavin_mOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020946">
        <property type="entry name" value="Flavin_mOase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050346">
        <property type="entry name" value="FMO-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23023">
        <property type="entry name" value="DIMETHYLANILINE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00743">
        <property type="entry name" value="FMO-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000332">
        <property type="entry name" value="FMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0274">FAD</keyword>
    <keyword evidence="2 6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="5 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9704531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="517" mass="58763" checksum="0C5319FF4F89DDD0" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E0R7</accession>
    <name>A0AA39E0R7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF241 domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_005219</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E0R7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048364">
        <property type="term" value="P:root development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048367">
        <property type="term" value="P:shoot system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004320">
        <property type="entry name" value="BPS1_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33070:SF109">
        <property type="entry name" value="DOMAIN PROTEIN, PUTATIVE (DUF241)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33070">
        <property type="entry name" value="OS06G0725500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03087">
        <property type="entry name" value="BPS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9703853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="289" mass="32386" checksum="960C0EE3518DF186" modified="2024-01-24" version="1">MANRCHVRSISLPARSHPTTLKIQEELNKLKKWEASSTSLGTICNGLSGMEELYKCLDELLSLQSTQQAISHHQHEKWVEELLDGSVSLLDVCGATRDVISEFKENVGNLQSALRRRKGDVCIESSINNYICSRKKMNKDAKKLLAAMKKMDNKAEAYPLLDQDHQLSTVIKVLRDVNAMSISIFQSLVLFLSTPVLKSKPSRWSLVSKLMQKGVVSCEEKHENVRELENIDFALSAISGERAELEKMQIAQKGLGALEVSIKGLDNGLECMFRHLIKTRASLLNIISH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E1N5</accession>
    <name>A0AA39E1N5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_003881</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9701857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E1N5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48017">
        <property type="entry name" value="OS05G0424000 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="126"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="193"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="285"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="326"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="389"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="412"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="451"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="503"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="8">
        <location>
            <begin position="39"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9701857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="590" mass="65228" checksum="F8C4E7FF90A02AA0" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E2S2</accession>
    <name>A0AA39E2S2_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_001637</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E2S2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35704">
        <property type="entry name" value="OS02G0254600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35704:SF1">
        <property type="entry name" value="OS02G0254600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9706178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="157" mass="18113" checksum="1F08C613CBB5438B" modified="2024-01-24" version="1">MHTSYLQAFNNHLFIRLLLLIPLNLDPSKQLSALNFSLEASFPRRKAGFLEPKIMGNCMETCRERHEEEEGERNQERQGEEEEKGSGYGVRVKIVLTKEELEWMMFQLKDKGGKSLEDVLRKIERGRSSAAAAEKVEGWKPSLESIMESPEVVEMER</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E441</accession>
    <name>A0AA39E441_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_002772</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E441"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14798">
        <property type="entry name" value="RX-CC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.430:FF:000003">
        <property type="entry name" value="Probable disease resistance protein At5g66910"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Ribonuclease Inhibitor"/>
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    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
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    </dbReference>
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        <property type="entry name" value="Apaf_helical"/>
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        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
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        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038005">
        <property type="entry name" value="RX-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF1193">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RPP13-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="5">
        <location>
            <begin position="6"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="4">
        <location>
            <begin position="164"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="6">
        <location>
            <begin position="435"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="7">
        <location>
            <begin position="574"/>
            <end position="868"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9704362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="890" mass="102325" checksum="0AF9F10BA9DBA545" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E4E8</accession>
    <name>A0AA39E4E8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Cellulose synthase-like protein G3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_001667</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E4E8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016760">
        <property type="term" value="F:cellulose synthase (UDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030244">
        <property type="term" value="P:cellulose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000135">
        <property type="entry name" value="Cellulose synthase-like protein G3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005150">
        <property type="entry name" value="Cellulose_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13301">
        <property type="entry name" value="X-BOX TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03552">
        <property type="entry name" value="Cellulose_synt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="512"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="546"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="583"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="632"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="669"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="699"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="138"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="437"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="276"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="300"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR605150-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR605150-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR605150-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9706240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="722" mass="81587" checksum="A8AC09BEB1FE858B" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E5E1</accession>
    <name>A0AA39E5E1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Glycosyltransferase 61 catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_003639</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E5E1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016763">
        <property type="term" value="F:pentosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049625">
        <property type="entry name" value="Glyco_transf_61_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007657">
        <property type="entry name" value="Glycosyltransferase_61"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20961">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20961:SF38">
        <property type="entry name" value="PROTEIN O-LINKED-MANNOSE BETA-1,4-N-ACETYLGLUCOSAMINYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04577">
        <property type="entry name" value="Glyco_transf_61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="36"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 61 catalytic" evidence="10">
        <location>
            <begin position="321"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9705667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="494" mass="56298" checksum="8AED520603E0EB68" modified="2024-01-24" version="1">MTKAPSESSKTHYSKQHHQRGSSTNISHRSFYSPKLSVYMLTACVILFILFQIQSLHSPGPPSSSSSSSSSPSWPLIHSWNSVSQESPNCSHELHALAEKLSDAVTFLPLKDLRYAQTAQQGHTWFMSSMHDTRVEGEVQYQQYPSAASRGRLLCIKGRDIHDGLWNSYALAWRDALPRNATLLKGLTFVSYNHYNYDNIWHGLSAMMPFVAWHLRQGQCAVPTRWVLYHWGELRMKMGPWVKSLMQATFGGSVNIEEFGDSGDEGVACFEEAVVMRHNEGGMSRERRLEVYDMMRCKAREYCNVRIEGRGLAVIGLTMLMRTGARSFRNESAVVRIFQRECRKVEGCRLTVAYSNNLTFCQQVKLMSLTNILVSPHGAQLTNMFLMDRNSNVMEFFPKGWLKLAGVGQYVYHWIASWSGMKHKGAWRDPNGDDCPYPEDDRRCMSIYKNGRIGYNDTFFEEWARNILVEVKTRKMEEALNKNNVVVLGGCACS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YGT5</accession>
    <name>A0AA38YGT5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mannan endo-1,4-beta-mannosidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_026621</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YGT5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051017">
        <property type="term" value="P:actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007163">
        <property type="term" value="P:establishment or maintenance of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00257">
        <property type="entry name" value="beta-trefoil_FSCN-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008999">
        <property type="entry name" value="Actin-crosslinking"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057232">
        <property type="entry name" value="DUF7910"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010431">
        <property type="entry name" value="Fascin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018087">
        <property type="entry name" value="Glyco_hydro_5_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10551">
        <property type="entry name" value="FASCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10551:SF9">
        <property type="entry name" value="FASCIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00150">
        <property type="entry name" value="Cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25490">
        <property type="entry name" value="DUF7910"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50405">
        <property type="entry name" value="Actin-crosslinking proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00659">
        <property type="entry name" value="GLYCOSYL_HYDROL_F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7910" evidence="6">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="5">
        <location>
            <begin position="171"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9670185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="464" mass="52588" checksum="4C4E7679260B60C7" modified="2024-01-24" version="1">MLDGTEVQFKSLMLQKYVSAENGGGMGVTVDKDVASSWETFRLWRVSDSEFQFRTSQGQFLTCDGEGGSVSAIAGSSSIKETFYVERNYDNRVHIKLKNGNYLQATVANQLSADYPGMPGWDDNAATFEMTIVANYIHGDYQLANGYGHDKAKEVLKRHRNSFITIEDFIFLNRHGINTVRIPVGWWIAFDPNPPAPFIGGTLEALDNAFSWAQAYNIRCIIDLHAAPGSQNGMEHSASRDGSTGWPTSASYISQTLDVIDFLASRYGKHPTLLGIELLNEPSAASVSLDLLVSYYKQGYQIVRKYSSKVYVIVCQRIGNADPSELFQADIGSYNLVVDLHYYNLFDNFFVNMSPLDNIQFIYNSRETQLQALNSANGPLVFIGEWVNEWNVTNATQLDYQNFGRTQLEVYNAASFGWSYWTLKNDRKHWDFEWNIRNNYLQLGRSLQSSFPLFVLKKHMHTHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YGY7</accession>
    <name>A0AA38YGY7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Guanosine nucleotide diphosphate dissociation inhibitor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_026654</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Regulates the GDP/GTP exchange reaction of most RAB proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the Rab GDI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YGY7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005093">
        <property type="term" value="F:Rab GDP-dissociation inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.405.10:FF:000002">
        <property type="entry name" value="Guanosine nucleotide diphosphate dissociation inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.519.10:FF:000015">
        <property type="entry name" value="Guanosine nucleotide diphosphate dissociation inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000158">
        <property type="entry name" value="Rab GDP dissociation inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.405.10">
        <property type="entry name" value="Guanine Nucleotide Dissociation Inhibitor, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.519.10">
        <property type="entry name" value="Guanine Nucleotide Dissociation Inhibitor, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018203">
        <property type="entry name" value="GDP_dissociation_inhibitor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000806">
        <property type="entry name" value="RabGDI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11787:SF10">
        <property type="entry name" value="GUANOSINE NUCLEOTIDE DIPHOSPHATE DISSOCIATION INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11787">
        <property type="entry name" value="RAB GDP-DISSOCIATION INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00996">
        <property type="entry name" value="GDI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00892">
        <property type="entry name" value="RABGDI"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00891">
        <property type="entry name" value="RABGDIREP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00053142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9670235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="445" mass="49911" checksum="8652A721C6731497" modified="2024-01-24" version="1">MDEEYDVIVLGTGLKECILSGILSVNGLKVLHMDRNDYYGGESTSLNLNQLWKRFRGDDKPPENLGSSKEYNIDMIPKFMMANGALVRVLIHTNVTKYLNFKAVDGSFVYNKGKIHKVPATDVEALKSPLMGLFEKRRARKFFIYVQDYDENDPKSHEKMDLRKVTAKEVISKYGLDDNTVDFIGHALAMYTDDKYLDKPALDFIKKMKLYAESLARFQGGSPYIYPLYGLGELPQAFARLSAVYGGTYMLNKPECKVEFDENGKAFGVTSEGETAKCKKVVCDPSYLPDKVGTVGKVARAICIMSHPIPNTSDSHSVQVILPQKQLGRKSDMYLFCCSYSHNVAPKGKYIAFVTTEAETDNPEMELKPGIELLGQVDEIFFDAYDRYEPANKQDDDNCFISTSYDATTHFESTVEDVIAMYSRITGKNLDLTVDLSAASAADEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YH02</accession>
    <name>A0AA38YH02_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Dof zinc finger protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_026685</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Transcription factor that binds specifically to a 5'-AA[AG]G-3' consensus core sequence.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YH02"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045174">
        <property type="entry name" value="Dof"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003851">
        <property type="entry name" value="Znf_Dof"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31992:SF362">
        <property type="entry name" value="DOF ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31992">
        <property type="entry name" value="DOF ZINC FINGER PROTEIN DOF1.4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02701">
        <property type="entry name" value="Zn_ribbon_Dof"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01361">
        <property type="entry name" value="ZF_DOF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50884">
        <property type="entry name" value="ZF_DOF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3 9" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 8 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Dof-type" evidence="11">
        <location>
            <begin position="35"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="300"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="300"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU369094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9670290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="340" mass="36631" checksum="C4BEB589063A78FB" modified="2024-01-24" version="1">MMSPDNIPAKPVSKDETQGSGGRKTGAHRPPDQVLKCPRCDSPNTKFCYYNNYSLTQPRHFCKTCRRYWTKGGALRNVPIGGGCRKNKKIKSSSRLSGDSKDVGASSEMGGLKFFHGLSPAMDFQLGGLPFPRLHSSTPPVYNQFSSFGDISATSAASAAVSTPSCFSIDQPGSSSSLMGFNYPLPSALKQGETGGFGGAIQDMGSMNVHSSLASSIESLSSINQDLHWKLQQQRLAMLFGGENQKDSSVSSVPPENQNQKLQPILFQNLEISKPEVCAVGNSRKDSPSGNPATEWFFDTYTPVTPTTTNTSTGGNGNDNTSHWNGIQAWSDLHQYSTLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YI91</accession>
    <name>A0AA38YI91_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Sugar phosphate transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_027073</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YI91"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004853">
        <property type="entry name" value="Sugar_P_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050186">
        <property type="entry name" value="TPT_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03151">
        <property type="entry name" value="TPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="80"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="135"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="158"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="188"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="221"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="255"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="283"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="Sugar phosphate transporter" evidence="7">
        <location>
            <begin position="25"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="304"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="319"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9670908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="337" mass="36981" checksum="09500EAB7C813492" modified="2024-01-24" version="1">MNMSPATKADKKATVDAAAWMFNVVTSVGIIIVNKALMATYGFSFATTLTGLHFATTTLMTTVLRWLGYIQGSHLPVSELLRFVLFANFSIVGMNVSLMWNSVGFYQIAKLSMIPVSCFLEVMLDKMRYSRDTKLSIALVLLGVAVCTVTDVSVNAKGFIAAFIAVWSTALQQYYVHFLQRKYSLGSFNLLGHTAPVQAASLLLLGPFLDYWLTNKRVDNYQYSLISVMFIILSCTIAVGTNLSQFICIGRFTAVSFQVIGHMKTILVLILGFLFFGKEGLNLHVVLGMIIAVVGMIWYGNASSKPGGKERRSPALPINKSQKTDGLSESTEIDGKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YJK1</accession>
    <name>A0AA38YJK1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">VQ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_025360</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YJK1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008889">
        <property type="entry name" value="VQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039611">
        <property type="entry name" value="VQ_4/11/13/19/31/33"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33402">
        <property type="entry name" value="VQ MOTIF-CONTAINING PROTEIN 11-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33402:SF46">
        <property type="entry name" value="VQ MOTIF-CONTAINING PROTEIN 4-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05678">
        <property type="entry name" value="VQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VQ" evidence="5">
        <location>
            <begin position="54"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="72"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="179"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="18"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="49"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="72"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9671612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="210" mass="22890" checksum="74E0D0F3E38FC481" modified="2024-01-24" version="1">MENTAKTQDRENPSPITSPNSNGSNCSSSSNSNGLQIQTPPLTPKPISRSDSNPYPTTFVQADANSFKQVVQRLTGSSKPTQDPPTKACNIPPIRTGQKKQGFKLYERRSQLKNGLMINPLVHGYVQNNSGFSPRKPEILSPSLLDFPKLVLSPVTPLTEDPFNKSSEEKAIAEKGFYLHPSPMSTPRDSEPQLLPLFPVTSPRVSGSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YJU3</accession>
    <name>A0AA38YJU3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Receptor-like serine/threonine-protein kinase</fullName>
            <ecNumber evidence="13">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PVL29_025486</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="13"/>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YJU3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048544">
        <property type="term" value="P:recognition of pollen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00028">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01098">
        <property type="entry name" value="PAN_AP_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000060">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.90.10.10:FF:000005">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000951">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.10">
        <property type="entry name" value="Bulb-type lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000858">
        <property type="entry name" value="S_locus_glycoprot_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024171">
        <property type="entry name" value="SRK-like_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002">
        <property type="entry name" value="RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD1-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002:SF1075">
        <property type="entry name" value="RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08276">
        <property type="entry name" value="PAN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00954">
        <property type="entry name" value="S_locus_glycop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000641">
        <property type="entry name" value="SRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00473">
        <property type="entry name" value="PAN_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57414">
        <property type="entry name" value="Hairpin loop containing domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 16" id="KW-0732">Signal</keyword>
    <keyword evidence="4 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041276218" description="Receptor-like serine/threonine-protein kinase" evidence="16">
        <location>
            <begin position="29"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="438"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="18">
        <location>
            <begin position="30"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="19">
        <location>
            <begin position="339"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="516"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="544"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR000641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAJ9671794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="831" mass="94415" checksum="252A4B82F7820384" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YLE4</accession>
    <name>A0AA38YLE4_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_026012</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YLE4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9672615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="109" mass="12043" checksum="F22D17910F6A3AA4" modified="2024-01-24" version="1">MEQNHRAPTVSKTILDNTIPEESNMDTVGISKDGLGKDLEPPGSLVNTYYLTDKGKSMVVSPSDVDDAAKERVWMERELRDPLTCWNADIEGPSTRGFESFPSSPVKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YLN1</accession>
    <name>A0AA38YLN1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DDE Tnp4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_026103</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HARBI1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YLN1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045249">
        <property type="entry name" value="HARBI1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930:SF280">
        <property type="entry name" value="OS11G0202600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE Tnp4" evidence="8">
        <location>
            <begin position="2"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9672750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="196" mass="22852" checksum="B12803837172EA78" modified="2024-01-24" version="1">MAVCDFDMIFRFVVVGWEGTAHDSRVLIETIRNPQHNFPMPPSEKYYLVDAAYTHTRGFMAPYRNVRYWLSDFRSGGKAVGKEEIFNQCHARLRNVIERAFGVVKAHFPILKRMAPYSFTTQTKIVMTCFSIHNFLRQISVADRLFSEYDNEVELESDNANQNQNLTTSSFFAASDQEFMQQFRNQIANELFQVFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YMS4</accession>
    <name>A0AA38YMS4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HMA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_023068</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YMS4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47856">
        <property type="entry name" value="HEAVY METAL-ASSOCIATED ISOPRENYLATED PLANT PROTEIN 20-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47856:SF2">
        <property type="entry name" value="OS08G0512200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMA" evidence="1">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9673244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="94" mass="10655" checksum="00D6375946353E8E" modified="2024-01-24" version="1">METVELKVEMVGIHEKRLRKCLAKLKGIEKVEVDANSQKVVVTGYAHRNKILKAVKRGGLKVDFWSAQNELLTAYASASYASYGSLRFNNFNFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YNH2</accession>
    <name>A0AA38YNH2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_023334</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YNH2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004709">
        <property type="term" value="F:MAP kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050538">
        <property type="entry name" value="MAP_kinase_kinase_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016">
        <property type="entry name" value="MAP KINASE KINASE KINASE SSK2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016:SF29">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="47"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9673710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="70" mass="7705" checksum="CDF42C0EBA336F0A" modified="2024-01-24" version="1">MQALFRIGKGEPPPVPDSLSPDARDFILKCLQVNPDDRPKAAQLLNHQFVKRPPPTSSGSASPLYHGRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YNZ2</accession>
    <name>A0AA38YNZ2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Rho GTPase-activating protein REN1-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_023500</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YNZ2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00821">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00159">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025757">
        <property type="entry name" value="MIP1_Leuzipper"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052799">
        <property type="entry name" value="Rho_GAP_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46265">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46265:SF21">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN REN1-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14389">
        <property type="entry name" value="Lzipper-MIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="4">
        <location>
            <begin position="33"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="5">
        <location>
            <begin position="187"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="394"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="722"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="861"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="592"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="397"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="450"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="474"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="492"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="727"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="738"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="783"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="829"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="895"/>
            <end position="907"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9673983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="937" mass="102463" checksum="8F2E56EC29A7EF19" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YQ27</accession>
    <name>A0AA38YQ27_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Patatin</fullName>
            <ecNumber evidence="7">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_023805</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Lipolytic acyl hydrolase (LAH).</text>
    </comment>
    <comment type="domain">
        <text evidence="7">The nitrogen atoms of the two glycine residues in the GGXR motif define the oxyanion hole, and stabilize the oxyanion that forms during the nucleophilic attack by the catalytic serine during substrate cleavage.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the patatin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="7"/>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YQ27"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004620">
        <property type="term" value="F:phospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07214">
        <property type="entry name" value="Pat17_isozyme_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1090.10:FF:000005">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32176:SF109">
        <property type="entry name" value="PATATIN-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32176">
        <property type="entry name" value="XYLOSE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6 7" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="5 6 7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNPLA" evidence="9">
        <location>
            <begin position="21"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="391"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXGXXG" evidence="6">
        <location>
            <begin position="25"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="6">
        <location>
            <begin position="63"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DGA/G" evidence="6">
        <location>
            <begin position="216"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9674472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="413" mass="44888" checksum="50005B5DE5A0440D" modified="2024-01-24" version="1">MGEGPKSPLQPPTYGNLITILSIDGGGIRGLIPGTVLGFLESELQKLDGEDARISDYFDVIAGTSTGGLVTAMLTTPNENTGRPLFSAKDIKDFYLDHCPKIFPQHSHDPIPHVTKVVTALSGPKYDGKYLHNLVKEKLGETRLHQTLTNVVIPTFDIKCLQPTIFSTYQVKSRPSLDALLSDICIGTSAAPTYLPAHYFETKDPAGGVREFNLIDGGVAANNPTLVAIGEVTKEIIRGSPDFFPIKPMDYGRFLVISLGTGSSKAEEKYNADEAAKWGVLGWLTSGGSTPLVKVFTQASGDMVDLHLSEVFQALHSEKSYLRIQDDTLSGITSSVDIATKENLDDLVKIGEELLKKRVSRVNLDTGIFEPSNHETNEEALTSFARLLSQEKQRRDTRSPHGHAAASKRLGVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YQJ6</accession>
    <name>A0AA38YQJ6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_023905</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Accepts the ubiquitin-like protein NEDD8/RUB1 from the ECR1-AXR1 E1 complex and catalyzes its covalent attachment to other proteins.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein neddylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YQJ6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019788">
        <property type="term" value="F:NEDD8 transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23794">
        <property type="entry name" value="UBCc_UBE2F_UBE2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000005">
        <property type="entry name" value="NEDD8-conjugating enzyme Ubc12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="9">
        <location>
            <begin position="29"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="7">
        <location>
            <position position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00058311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9674654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="200" mass="22716" checksum="5EBDA645FB2ADF1B" modified="2024-01-24" version="1">MIRLFKEKEKLRETAESSDGRAPVKKLSAGELRLHKDISELNLPESCGISFPNGKDDLLNFEITIRPDEGFYGGGDFVFSFKVPLMYPHEAPKVKCKTKVYHPNIDLAGNVCLNILREDWNPVLNINTITYGLYHLFTQPSHEDPLNPDAAAVLRASSQAFQTVVRHVMTNGYRGETYYPQLMQAMQHTKAPSRHQSRLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YRK4</accession>
    <name>A0AA38YRK4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">laccase</fullName>
            <ecNumber evidence="5">1.10.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PVL29_024320</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>4 hydroquinone + O2 = 4 benzosemiquinone + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:11276"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17594"/>
            <dbReference type="ChEBI" id="CHEBI:17977"/>
            <dbReference type="EC" id="1.10.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Secreted</location>
            <location evidence="3">Extracellular space</location>
            <location evidence="3">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the multicopper oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.10.3.2" evidence="5"/>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YRK4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052716">
        <property type="term" value="F:hydroquinone:oxygen oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046274">
        <property type="term" value="P:lignin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13849">
        <property type="entry name" value="CuRO_1_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13875">
        <property type="entry name" value="CuRO_2_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034288">
        <property type="entry name" value="CuRO_1_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034285">
        <property type="entry name" value="CuRO_2_LCC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF410">
        <property type="entry name" value="LACCASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0052">Apoplast</keyword>
    <keyword evidence="11" id="KW-0186">Copper</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0439">Lignin degradation</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0964">Secreted</keyword>
    <keyword evidence="14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="17">
        <location>
            <begin position="35"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="15">
        <location>
            <begin position="156"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="16">
        <location>
            <begin position="397"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ9675319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="486" mass="54557" checksum="24A3DD30354A7A25" modified="2024-01-24" version="1">MELIKKKKKILLLELLGFLILTVLTFCMAAILLQVKETSYTWLRSTKKILTVNGLFPGPPIYMRRGDRLNINVRNKGKYNHGVKQPGNPWSDGPNYVTQCPIKLGANFTYEIVLSSEEGTLWWHAHSDWSRSTVHGPLIVLPQLGKIYPFAKPHKEVPIVLASWFRGDVMEIIETALQNGGEPNQSDAFTINGQPGDLYDCSTEGTFKMVVDYGKTYLLRIINAIMNKEMFFMVASHSLTVVGTDGAYIKPINTSYIMITPGQTMDVLITADQYPSYYYMASQFTVRLRSLANEDYPVDVPQTVDTHMYITISMNTLPCVNNSCDGPDGSRLASILNKISFVEPSIDVILAYYKGINGVYDTYFPNEPPYYFNFTGDDLPDTLLTPERGTKVKVLDYNSNVEIVLVDPPEVNTVGVPKNGWAAIRFRADNYECITTHTYLYMGMVHALSFGIERHASWGMDTVLIVKNGTTSSTSMLEPPSYLNPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YS25</accession>
    <name>A0AA38YS25_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_024379</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YS25"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000009">
        <property type="entry name" value="transcription factor MYB1R1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000154">
        <property type="entry name" value="Transcription factor SRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006447">
        <property type="entry name" value="Myb_dom_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01557">
        <property type="entry name" value="myb_SHAQKYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44042:SF15">
        <property type="entry name" value="DUPLICATED HOMEODOMAIN-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44042">
        <property type="entry name" value="DUPLICATED HOMEODOMAIN-LIKE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Myb-like" evidence="7">
        <location>
            <begin position="12"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="7">
        <location>
            <begin position="102"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="103"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="8">
        <location>
            <begin position="105"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="83"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9675434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="333" mass="38811" checksum="5C175569D17F4E7A" modified="2024-01-24" version="1">MDCDPFPSQFIRTHWTRLEDKIFEQALVIFPEEMPDRWQSIAQQLQGKTPEDMKLHYELLVEDVTNIENGNVEMPSYLEEAWRRETEPRTSHDSVGKRTKEVERKKGTPWTEVEHRLFLSGLVRFGKGDWRSISRHVVITRTPTQVASHAQKFYLRQNSVKKERKRSSIHDINTIDNFSSSDFPNNFSDQPKDEVIDMPDNFSDLPNNFPDQQQVHHFPNNFADQQAQDINTTLNNYPNYSTNFLDQQAQDINNTVLDNFPTNFPDQQAQDINTTLQNFPNYPTNFPDQQGQQVQHAQGEGIGYPTNFPELQLQTFRQQGGGGFQTFTHLFSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YU34</accession>
    <name>A0AA38YU34_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_021866</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9676562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YU34"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056712">
        <property type="entry name" value="DUF7810"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35736:SF3">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35736">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25102">
        <property type="entry name" value="DUF7810"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9676562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="588" mass="66146" checksum="DD55CB0487840407" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YUD7</accession>
    <name>A0AA38YUD7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Alpha-dioxygenase 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_022055</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9676861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YUD7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016702">
        <property type="term" value="F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001676">
        <property type="term" value="P:long-chain fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031408">
        <property type="term" value="P:oxylipin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09818">
        <property type="entry name" value="PIOX_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.640.10:FF:000011">
        <property type="entry name" value="Alpha-dioxygenase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.640.10">
        <property type="entry name" value="Haem peroxidase domain superfamily, animal type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034815">
        <property type="entry name" value="A_dioxygenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019791">
        <property type="entry name" value="Haem_peroxidase_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037120">
        <property type="entry name" value="Haem_peroxidase_sf_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050783">
        <property type="entry name" value="Oxylipin_biosynth_metab"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11903:SF25">
        <property type="entry name" value="ALPHA-DIOXYGENASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11903">
        <property type="entry name" value="PROSTAGLANDIN G/H SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03098">
        <property type="entry name" value="An_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50292">
        <property type="entry name" value="PEROXIDASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="12" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="4 13" id="KW-0349">Heme</keyword>
    <keyword evidence="10 13" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0925">Oxylipin biosynthesis</keyword>
    <keyword evidence="3" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="13">
        <location>
            <position position="382"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR619791-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9676861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="634" mass="72324" checksum="59557413B8EC5AF2" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YUH8</accession>
    <name>A0AA38YUH8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FAD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_022000</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9676767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YUH8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028348">
        <property type="entry name" value="FAD-binding_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049516">
        <property type="entry name" value="FAD-depend_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42842">
        <property type="entry name" value="FAD/NAD(P)-BINDING OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42842:SF3">
        <property type="entry name" value="FAD_NAD(P)-BINDING OXIDOREDUCTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21688">
        <property type="entry name" value="FAD-depend_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00419">
        <property type="entry name" value="ADXRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding" evidence="1">
        <location>
            <begin position="224"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-dependent protein C-terminal" evidence="2">
        <location>
            <begin position="418"/>
            <end position="633"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF21688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9676767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="707" mass="77697" checksum="E059ABC7CB83B921" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YVW5</accession>
    <name>A0AA38YVW5_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_022466</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YVW5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47487">
        <property type="entry name" value="OS06G0651300 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47487:SF20">
        <property type="entry name" value="ZINC FINGER, U1-TYPE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="U1-type" evidence="3">
        <location>
            <begin position="91"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="94"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="U1-type" evidence="3">
        <location>
            <begin position="136"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="139"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="U1-type" evidence="3">
        <location>
            <begin position="183"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="186"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="43"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9677488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="238" mass="27273" checksum="D8D20B5A78646CA3" modified="2024-01-24" version="1">MDDGRRGDVMPLELAMKRESAYRKKVEMMLLQLYSGESREDPFSSQVPSSSPSSGPSLSGTKRKTPTRSSQCLPPQQLQLLYSGHTLGNRKDDLFCKVCQVTCPSSFHLKQHLIGQKHKDTLDLKSGRNIREEEANTRKWCDLCKIWCTNKYSLEQHFKGKKHQDNVQELELGGKEGQGKRNQVKLYCELCKLWCMDEYAFNQHLKGKKHILNLHAFEEKRTAKGKGLAVDQSSTRKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YYI2</accession>
    <name>A0AA38YYI2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein PAM68, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_021038</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YYI2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021855">
        <property type="entry name" value="PAM68-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34575">
        <property type="entry name" value="PROTEIN PAM68, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34575:SF1">
        <property type="entry name" value="PROTEIN PAM68, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11947">
        <property type="entry name" value="DUF3464"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="140"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9678994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="198" mass="22384" checksum="52D0C33D84725841" modified="2024-01-24" version="1">MECCSLSTTTPLISPPSSTPALYISQFPIPTPKLYPKNRLLHSTPLCASLNRPKGFGPSPRKTEKRKKLKKNYSDDEDEEEEEEEEEGAEAEEGVIPEIVTNRMISRMGFSVGIPLMIGLLFFPFFYYLKVGLKIDVPTWVPFIVSFFFFGSALLGVSYGIVSSSWDPLREGSFLGWTEAQKNWPVFWQSLWGRSGKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z059</accession>
    <name>A0AA38Z059_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA-binding protein BIN4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_021696</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z059"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009330">
        <property type="term" value="C:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051276">
        <property type="term" value="P:chromosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042023">
        <property type="term" value="P:DNA endoreduplication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033246">
        <property type="entry name" value="BIN4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34810">
        <property type="entry name" value="DNA-BINDING PROTEIN BIN4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34810:SF1">
        <property type="entry name" value="DNA-BINDING PROTEIN BIN4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="357"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="140"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="399"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9679864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="428" mass="47266" checksum="7D6A92F811899528" modified="2024-01-24" version="1">MSRDSPIKRVEGACCLPAPVRRTLLSPQVKMGSSREESPDWLHCFQAPTHSALTLSSDSDSPPDDSPLREDKDADSPFNETSKAKSPRKRPRAEGPKSTKKQKVNNHKKKKGNEGRKKASVEKPSDGEPNAPNHSVWMLSSDSESCPDNSSMREDYIHHEESSVHKTTQFPDKEKDAVLSENGQESLLNEASKEKSPKKRVKVEDHISSKKKKANSDMKGKGHDDGMELLEEEASDKHTEPQVSSSGLPLVLSEKVHRSKALVECEGESIDLSGDMGAVGRIVILDTPSRNHEMFLDLKGTIYKTTIVPSRTFCIVSFGQSEAKVEAVMNDFIQLKPQSNVYEAETMVEGILEGFSFDSDDEADKMPKASTRQTDQNEGDEEQTNGKTKGKAEKASGVVRKKGKTAGAKPPKKVRKKAQVQKRTKTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z3Z1</accession>
    <name>A0AA38Z3Z1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Disease resistance protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_020389</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Confers resistance to late blight (Phytophthora infestans) races carrying the avirulence gene Avr1. Resistance proteins guard the plant against pathogens that contain an appropriate avirulence protein via an indirect interaction with this avirulence protein. That triggers a defense system including the hypersensitive response, which restricts the pathogen growth.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9681494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z3Z1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009626">
        <property type="term" value="P:plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14798">
        <property type="entry name" value="RX-CC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038005">
        <property type="entry name" value="RX-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF1152">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0381">Hypersensitive response</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0611">Plant defense</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="9">
        <location>
            <begin position="6"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="8">
        <location>
            <begin position="173"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="10">
        <location>
            <begin position="429"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="11">
        <location>
            <begin position="548"/>
            <end position="871"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9681494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="915" mass="105531" checksum="008D49BA38EEFA71" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z4I5</accession>
    <name>A0AA38Z4I5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">GTP-binding protein SAR1A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_017922</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the small GTPase superfamily. SAR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9681779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z4I5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00879">
        <property type="entry name" value="Sar1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000161">
        <property type="entry name" value="Small COPII coat GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006687">
        <property type="entry name" value="Small_GTPase_SAR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45684">
        <property type="entry name" value="RE74312P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51422">
        <property type="entry name" value="SAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11 14" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="130"/>
            <end position="133"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR606687-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9681779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="194" mass="22297" checksum="F7AA3C72F4698380" modified="2024-01-24" version="1">MSKVFFKEQFEFLFLFAISPRNGGWMIFSLMFVWLIGVDIGVLQRLVQHQPTQYPTSEELSIGKIKFKAFDLGGHQIARRVWKDYYAKVDAVVYLVDAYDKERFAESKKELDALLSDEALANVPFLVLGNKIDIPYAASEDELRYHLGLTNFTTGKGRVNLADSNVRPLEVFMCSIVRKMGYGDGFKWLSQYIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z5R7</accession>
    <name>A0AA38Z5R7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ASCH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_018370</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z5R7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06554">
        <property type="entry name" value="ASCH_ASC-1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.130.30:FF:000002">
        <property type="entry name" value="Activating signal cointegrator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.30">
        <property type="entry name" value="Hypothetical protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007374">
        <property type="entry name" value="ASCH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039128">
        <property type="entry name" value="TRIP4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12963:SF0">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12963">
        <property type="entry name" value="THYROID RECEPTOR INTERACTING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04266">
        <property type="entry name" value="ASCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01022">
        <property type="entry name" value="ASCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ASCH" evidence="2">
        <location>
            <begin position="14"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="272"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="285"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9682439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="323" mass="36390" checksum="B4EDFF1F142F8D3B" modified="2024-01-24" version="1">MTERNSWNYRNPCLTMHQPWASLLVYGIKRIEGRSWPAPIRGRLWIHAASKVPDEATVKAMEEFYREIYAVDGIYDLKFPENYPVSRLLGCVQVVGCSKREELACWKEVSEGVRLEALTDFCWLCEQPQKLLVPFEMRGNQGVYDLERKIYEAAVRGLAPVESPLPIKFPLPNPRNPFSLKPGSIFTRFPESKATEMEKSSALSSAIAGAQAAATQFSKKDQSSHNDAIQNRNIINAKRIGQFESKSWENNKSPAGNFNKGSNRGTQLFVKQGQSSYSEHDGRISSHNQLPRAGSNQHLRPPSKVCKSWTITVGIDLCSSLER</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z763</accession>
    <name>A0AA38Z763_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Anther-specific protein BCP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_019308</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z763"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041233081" description="Anther-specific protein BCP1" evidence="3">
        <location>
            <begin position="22"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="91"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9683676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="110" mass="10451" checksum="55A734FCFFEA8866" modified="2024-01-24" version="1">MAPKLLILALLFMAVVASTSAAAPAKAPEAASDDEIGEVGGDDSGDGGLSPSSDVVDAPVGGPVTEGAFSNLGPSAATGGNKENGAAELEVSAVAAGAAAVAAGFFFLKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z7A4</accession>
    <name>A0AA38Z7A4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_019347</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z7A4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008353">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07840">
        <property type="entry name" value="STKc_CDK9_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000043">
        <property type="entry name" value="probable serine/threonine-protein kinase At1g54610"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000021">
        <property type="entry name" value="probable serine/threonine-protein kinase At1g54610"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF188">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE C-2 C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="141"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="32"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="455"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="54"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="77"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9683731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="506" mass="56980" checksum="E61C3FDE168AA3CD" modified="2024-01-24" version="1">MGCISSKQAVSASPDFDISEAIEDDWSVISASSIKQRRGSGGASVKKANKVRKERKEEKEEKEKDKDKESEEEEEERKDNEGLELQRSKKGSVNGKSTFSVRFGNLHKVEAEQIIAGWPSWLSAAAGEAIHGWLPLRADSFEKLEKIGQGTYSTVYRARDVETGRIVALKKVRFDNFQPESVMFMSREITILRRLDHPNIMKLEGIITSRLSCSIYLVFEYMEHDLAGLVSCPDIKFSVAQVKCYMQQLLSAIEHCHLLGVMHRDIKASNILVNNEGVLKLADFGLANILRPKHKQILTSRVVTLWYRPPELILGSTSYGVSVDLWSVGCVFAELLIGKPLFKGRTEVEQLHKIFKLCGSPPDEYWKKSKFPHAAMFKPHHPYESTLRERFREFPTTALNLIETLLSVEPPKRGTASSALISEFFNTKPYACEPSSLPKYPPNKEIDAKCREEARRKTGGVGVRGSGVLRKPRRSRTSLQEPNSTSKFAVTEVLFHSLTIIPAVII</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z7Q7</accession>
    <name>A0AA38Z7Q7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_016391</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9683869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z7Q7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04873">
        <property type="entry name" value="ACT_UUR-ACR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11455">
        <property type="entry name" value="bHLH_AtAIG1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045847">
        <property type="entry name" value="AIG1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45844">
        <property type="entry name" value="TRANSCRIPTION FACTOR BHLH30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45844:SF18">
        <property type="entry name" value="TRANSCRIPTION FACTOR BHLH51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="7">
        <location>
            <begin position="108"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="97"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="97"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9683869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="297" mass="33168" checksum="5C860AC1B550997B" modified="2024-01-24" version="1">MGTISTTRCSEIEFTEWLIGSRINLLSPLYGGRFVAGYERGCLLDMENCLYSGWAQEADCTQCDAAPVLPWLPPPHVSASFQFHGFPSWSLPVEKMATEDRSDDRTATASKSHSQAEKRRRDRINAQLATLRKLIPKSEKMDKAALLGSVIDHVKDLKRQAMEVSDVFTVPTEVDEVTVDCEFDQGLVPSNTYKTPENIFIKASVCCEDRPELFSELIRALQGLKLTTVRADMASLSGRTKSILVLCSKDDKNSVCISTLKQSLKVVLSRIVSSSTASNYRITSKRQRFFLPSNFPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z8K7</accession>
    <name>A0AA38Z8K7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Cucumisin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_016718</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the peptidase S8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z8K7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02120">
        <property type="entry name" value="PA_subtilisin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04852">
        <property type="entry name" value="Peptidases_S8_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000006">
        <property type="entry name" value="Subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.80:FF:000002">
        <property type="entry name" value="Subtilisin-like protease SBT5.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034197">
        <property type="entry name" value="Peptidases_S8_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010259">
        <property type="entry name" value="S8pro/Inhibitor_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045051">
        <property type="entry name" value="SBT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041469">
        <property type="entry name" value="Subtilisin-like_FN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10795">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17766">
        <property type="entry name" value="fn3_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05922">
        <property type="entry name" value="Inhibitor_I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 7" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041468946" description="Cucumisin" evidence="8">
        <location>
            <begin position="19"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="domain" description="Inhibitor I9" evidence="10">
        <location>
            <begin position="38"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="9">
        <location>
            <begin position="135"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="domain" description="Subtilisin-like protease fibronectin type-III" evidence="11">
        <location>
            <begin position="639"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6 7">
        <location>
            <position position="143"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6 7">
        <location>
            <position position="207"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6 7">
        <location>
            <position position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9684393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="742" mass="78393" checksum="13BC7CD8823D4871" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z9H1</accession>
    <name>A0AA38Z9H1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ACT domain-containing protein ACR</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Protein ACT DOMAIN REPEATS</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_016936</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Binds amino acids.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z9H1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040217">
        <property type="entry name" value="ACR1-12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056816">
        <property type="entry name" value="ACR2/9/10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056805">
        <property type="entry name" value="ACT_ACR9/10_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31096:SF74">
        <property type="entry name" value="ACT DOMAIN-CONTAINING PROTEIN ACR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31096">
        <property type="entry name" value="ACT DOMAIN-CONTAINING PROTEIN ACR4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24914">
        <property type="entry name" value="ACR10_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24931">
        <property type="entry name" value="ACT_ACR9_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24926">
        <property type="entry name" value="ACT_ACR9_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ACT" evidence="3">
        <location>
            <begin position="115"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU369043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9684710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="415" mass="47129" checksum="849523E36FDA2666" modified="2024-01-24" version="1">MGILYDYEDVVLIRQAEKPGDPTEITVNCPDKIGLGCDLCRIILRFGLSISRGDSSTDGKWCYILFWVVGKPNTRWNLLHKRLLEVCPSCSSASGISYYKPEFHQQPKPPDVFLLKFWCYYDRKGLLHDITEVLCELELTIKRVKVSTAPDGRVMDLFFVTDTRNELHTQKRRDEAIYHLKDVLGDAMISCEIELAGPEVTGCSQGSSFLPPAITEEIFSLELPNGHQNGSPASNTLIVTMDNSLSPSHTHVHIICQDQKGLIYDIMRTLKDYNIQTSYGRFFANAKGIFEADLLVMQADGKKLVDPNKQNALSSRLKMELFRPLRVAVQSRGPDTELLVANPVELCGRGRPLVFYDITLALKILKIQIFSVEIGRHMIQDREWEVYRILLDEEEGSCVSRNKIEEGVRKKLMGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZA49</accession>
    <name>A0AA38ZA49_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_017186</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF78">
        <property type="entry name" value="OS12G0587133 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="483"/>
            <end position="763"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9685055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1422" mass="163862" checksum="0F707349A486E717" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZB27</accession>
    <name>A0AA38ZB27_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">ADP/ATP translocase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">ADP,ATP carrier protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_017670</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">ADP:ATP antiporter that mediates import of ADP into the mitochondrial matrix for ATP synthesis, and export of ATP out to fuel the cell. Cycles between the cytoplasmic-open state (c-state) and the matrix-open state (m-state): operates by the alternating access mechanism with a single substrate-binding site intermittently exposed to either the cytosolic (c-state) or matrix (m-state) side of the inner mitochondrial membrane.</text>
    </comment>
    <comment type="function">
        <text evidence="16">Catalyzes the exchange of ADP and ATP across the membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ADP(in) + ATP(out) = ADP(out) + ATP(in)</text>
            <dbReference type="Rhea" id="RHEA:34999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:35000"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="3 16">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZB27"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005471">
        <property type="term" value="F:ATP:ADP antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140021">
        <property type="term" value="P:mitochondrial ADP transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990544">
        <property type="term" value="P:mitochondrial ATP transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000001">
        <property type="entry name" value="ADP,ATP carrier protein, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002113">
        <property type="entry name" value="ADT_euk_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45635:SF41">
        <property type="entry name" value="ADP,ATP CARRIER PROTEIN 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45635">
        <property type="entry name" value="ADP,ATP CARRIER PROTEIN 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00927">
        <property type="entry name" value="ADPTRNSLCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0050">Antiport</keyword>
    <keyword evidence="11 14 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 14 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 15" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="187"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="252"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="291"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="14">
        <location>
            <begin position="84"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="14">
        <location>
            <begin position="189"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="14">
        <location>
            <begin position="289"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU368008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9685725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="386" mass="42249" checksum="2F4B682E83DD69D2" modified="2024-01-24" version="1">MIDQPQYPTIRQKVAGHLHHSQNIQAFDGRFQQPALHQRHYSAYGNYTNAALQYPMCRATCDLSMVGSNVSPVFVPAPKEKGFAGFAIDFLMGGVSAAVSKTAAAPIERVKLLIQNQDEMIKTGRLSEPYKGIGDCFSRTIKDEGFMSLWRGNTANVIRYFPTQALNFAFKDYFKRLFNFKKDRDGYWKWFAGNLASGGAAGASSLLFVYSLDYARTRLANDAKAAKKGGERQFNGLVDVYRKTLKSDGVAGLYRGFNISCVGIIVYRGLYFGMYDSLKPVVLTGGLQDSFFASFALGWLITNGAGLASYPIDTVRRRMMMTSGEAVKYKSSFDAFAQILKNEGAKSLFKGAGANILRAVAGAGVLAGYDKLQVIVFGKKYGSGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZCL5</accession>
    <name>A0AA38ZCL5_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_015011</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9685919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZCL5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008962">
        <property type="term" value="F:phosphatidylglycerophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000148">
        <property type="entry name" value="Haloacid dehalogenase superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006549">
        <property type="entry name" value="HAD-SF_hydro_IIIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027706">
        <property type="entry name" value="PGP_Pase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010021">
        <property type="entry name" value="PGPP1/Gep4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039923">
        <property type="entry name" value="Protodermal_1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01662">
        <property type="entry name" value="HAD-SF-IIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01668">
        <property type="entry name" value="YqeG_hyp_ppase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33210">
        <property type="entry name" value="PROTODERMAL FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33210:SF18">
        <property type="entry name" value="PROTODERMAL FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09419">
        <property type="entry name" value="PGP_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="354"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="386"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="415"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9685919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="598" mass="65201" checksum="C034F95383D55C7B" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZDA0</accession>
    <name>A0AA38ZDA0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_015528</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZDA0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd17039">
        <property type="entry name" value="Ubl_ubiquitin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="1">
        <location>
            <begin position="6"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="1">
        <location>
            <begin position="107"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9686720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="260" mass="29962" checksum="3B6858CF972060E2" modified="2024-01-24" version="1">MDRDSVIVHIEGLEEVVSFSIEIPSAATFRQLKEKIKEKTGVMVERQLILPKDGRVPMNDNSTLESRGFKHLAELVLKLLVEIFTGKFFYIEVGDDAKVADLKRDLVTFTVEGSEEVVKFRIHIPQSATILQLRERIQQYTGVDLDRQIIFDKETRLLWLDFQTVKECGYTSSGTIFLLVLPLPLGAQLCIKISSWAAEFHLIVQDNDTVSDLKKKIRTERKFSSEKRINLFHLFTEMHDHDPLYKYYVNVGSTITMTHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZGI2</accession>
    <name>A0AA38ZGI2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Actin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_013900</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the actin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9687899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZGI2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000218">
        <property type="entry name" value="actin, alpha sarcomeric/skeletal-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000205">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000047">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000150">
        <property type="entry name" value="Actin-related protein 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020902">
        <property type="entry name" value="Actin/actin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004001">
        <property type="entry name" value="Actin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00190">
        <property type="entry name" value="ACTIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00406">
        <property type="entry name" value="ACTINS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01132">
        <property type="entry name" value="ACTINS_ACT_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9687899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="376" mass="42113" checksum="BC41512C202ED560" modified="2024-01-24" version="1">MDFDEKQAVVCDNGSGLVKAGFAGDDAPCVVFPSVIGWPRNKHAMIGIGQKEMYLGDEAQARRGVLRLSYPIDHGMVRDWEAMERLWQHTFEKELRVSIEERPVLLTEPPLNPKFNKEKMVEIMFEAFEIPATYVAIQAVLSLYASGRTTGIVMDSGEGVTHVVPIYEGYALPHAIQRLDLAGRDLTDYLTKILTEEGYVFTTSAEREIVKDIKEQTTYVAMDFEKELATSRESLELDKQYELPDGQVITIGTGRFKCPEILFDPSKVGIESCGVHDIVLKSIRRCDMDIRRDMYGNVVLSGGTTMVPGLADRLAKELSSLAPPGVRVRVVAPPERKYSVWIGGSILASLSTFEQMWIKKEEYMESGSSIVHMKCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZJG8</accession>
    <name>A0AA38ZJG8_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_012575</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9689974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZJG8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9689974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="85" mass="9289" checksum="DB24617ECDCA1B41" modified="2024-01-24" version="1">MPQIHQILKTQVGKPKASKLRQHSYDARYASLVKIAKCLDSCEATEEDVSQVLRRLGDKLLEQDAVIVLSIPAGKESQKKVGGGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZKG5</accession>
    <name>A0AA38ZKG5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7705 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_013091</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZKG5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056122">
        <property type="entry name" value="DUF7705"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33916">
        <property type="entry name" value="EXPANSIN-LIKE EG45 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33916:SF12">
        <property type="entry name" value="NEPROSIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24804">
        <property type="entry name" value="DUF7705"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041407091" description="DUF7705 domain-containing protein" evidence="1">
        <location>
            <begin position="24"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7705" evidence="2">
        <location>
            <begin position="34"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9690756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="508" mass="56875" checksum="967E820DCEC55E09" modified="2024-01-24" version="1">MEKSKNPCCIAAVLLLVVGYVSGVLDSWEEAGCVSGVGDPGMKRDGLRLAIESWNQCNEVGDETPNMGSPRAADCFDVYKSAQLLNTQKKCSVCNAIPRLLVHRVTEEDNKLGVGQPFPGVQPEAVNNVDLYAAEKEIYLGSKCQVEDTPKPWQFWMIMLKSGNMDTYAAKCPKNGHKVGPYGPDDRFPCFGKGCMNHPLIYHNYTTLRGSTMKGRFYGSWDLDADLCKGMVGNISYHSVTWEKQLGEGSWVFHHVLRTSTKYPWLMLYLRSDATTGFSGGYHYPTRGMSKIIPESPYFKVTFTLNVIQGGGPSSQFYLMDIGSCWKNNGQPCDGDVTSDVTRYSEMIINPNTSALCRPDNLNVCPPYHTFPNGTRVHRNDTARYPYAAYHFYCSPGNGQHLEEPYNLCDPFSNPQPQEILQILPHPVWGDYGYPTKQGEGWIGDSRTWELDVGRLSQSLYFYQDPGTPPARRLWMSVDLGTEIYKDADQIAEWTVSGFDILVPANPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZKR8</accession>
    <name>A0AA38ZKR8_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_013033</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZKR8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02248">
        <property type="entry name" value="Peptidase_C1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000023">
        <property type="entry name" value="Senescence-specific cysteine protease SAG39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025661">
        <property type="entry name" value="Pept_asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025660">
        <property type="entry name" value="Pept_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039417">
        <property type="entry name" value="Peptidase_C1A_papain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00705">
        <property type="entry name" value="PAPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00640">
        <property type="entry name" value="THIOL_PROTEASE_ASN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00639">
        <property type="entry name" value="THIOL_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0788">Thiol protease</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041396759" evidence="7">
        <location>
            <begin position="25"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="9">
        <location>
            <begin position="36"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="8">
        <location>
            <begin position="122"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9690664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="340" mass="37213" checksum="F1A9D9277C4864D9" modified="2024-01-24" version="1">MALESKIICITLLIMGIWASQALSRTLHEVSMSERHEDWMGLYGRTYKDIAEKERRFKIFKENVEYIESVNSAGNRRYKLSINEFADQTNEEFRASRNGYKMSSRPRSSEITSFRYENVAAVPSSMDWRKKGAVTPIKDQGQCGCCWAFSAVAAMEGITQLKTGKLISLSEQELVDCDTSGEDQGCEGGLMDSAFEFIIGNGGLTTEANYPYKGVDATCNKKKAASSAAKIKNYEDVPANSEAALLNAVAQHPVSVAIDAGGSDFQFYSSGVFTGQCGTELDHGVTAVGYGKTDDGTKYWLVKNSWGTGWGEDGYIRMERDIGADEGLCGIAMEASYPTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZMC2</accession>
    <name>A0AA38ZMC2_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_013616</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZMC2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9691489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="117" mass="13384" checksum="35D65B52BDDA70F6" modified="2024-01-24" version="1">MDHFFTLDAEMVVQNRKSGKMNQTTINDAYKKEARERACMLITRWMYEAAIPFNAVTYPSFQPMIEAIGQYGVGMKGPTLHEVRVTNLKKELALTKDLMKDHMMEWGKNGCSIMSDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZNU0</accession>
    <name>A0AA38ZNU0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_011454</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZNU0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000438">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039537">
        <property type="entry name" value="Retrotran_Ty1/copia-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057670">
        <property type="entry name" value="SH3_retrovirus"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648">
        <property type="entry name" value="TRANSPOSASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648:SF28">
        <property type="entry name" value="TRANSPOSON-ENCODED PROTEIN WITH RIBONUCLEASE H-LIKE AND RETROVIRUS ZINC FINGER-LIKE DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25597">
        <property type="entry name" value="SH3_retrovirus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="3">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9692402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="524" mass="59154" checksum="CA16CE33DCAE9796" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZPW3</accession>
    <name>A0AA38ZPW3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Complex III subunit II</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Core protein II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_011336</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Substrate recognition and binding subunit of the essential mitochondrial processing protease (MPP), which cleaves the mitochondrial sequence off newly imported precursors proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the peptidase M16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZPW3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000022">
        <property type="entry name" value="mitochondrial-processing peptidase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000008">
        <property type="entry name" value="Mitochondrial-processing peptidase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050361">
        <property type="entry name" value="MPP/UQCRC_Complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001431">
        <property type="entry name" value="Pept_M16_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851">
        <property type="entry name" value="METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851:SF203">
        <property type="entry name" value="MITOCHONDRIAL-PROCESSING PEPTIDASE SUBUNIT ALPHA-1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00143">
        <property type="entry name" value="INSULINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="11">
        <location>
            <begin position="88"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="12">
        <location>
            <begin position="242"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00075935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9692218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="506" mass="54621" checksum="CAA0B843C65B1DD6" modified="2024-01-24" version="1">MYRTAASRLRALKGHGGNWRTARFASSSAVTARSSSSPGLFSWLTGEKSSSLSPLNLPLAGVSLPPPLPDYVEPSKTKITTLSNGVKIASETSPNPAASIGLYVDCGSIYETPLSFGATHLLERMAFKSTTNRSHLRVVREVEAIGGNVTASASREQMGYTFDALKTYVPEMVELLVDCVRNPVFLDWEVNEQLQKVKAELGELSNNPQGLLLEAIHSAGYSGALANPLLAPESAINRLNSTILEEFVAENYTAPRMVLAASGVEHEELLSIAEPLVSDLPSVPRPEEPKSVYVGGDYRCQADSGITHLALAFEVPGGWHNEKEAITLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNEYQQLQSFSAFNNIFNNTGIFGIYASTGSDFVAKAVDIAAGELLSIASPGQVDQVQLNRAKEATKSAVLMNLESRMIASEDIGRQILTYGERKPLEHFLKAVDEITLKDITTIGQRIISSPLTMASYGDVIHVPSYESVNRKFHSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZR47</accession>
    <name>A0AA38ZR47_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_009625</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9693756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZR47"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9693756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="127" mass="14735" checksum="652621603C0368FA" modified="2024-01-24" version="1">MAATDVAVHLYWNGRVKTKKYDVVYEGDDMNVMPIKLKHGTTYFGLLDKIYATTGIRRHDGWNVVCRYPISSREYKPIPIKNDEAVDLMLEVPCRSFRVYCVEIYLEATRCVECPHPELVESYRVVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZRQ1</accession>
    <name>A0AA38ZRQ1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Macrophage migration inhibitory factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_009850</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MIF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZRQ1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050178">
        <property type="term" value="F:phenylpyruvate tautomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.429.10:FF:000004">
        <property type="entry name" value="Tautomerase/MIF superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.429.10">
        <property type="entry name" value="Macrophage Migration Inhibitory Factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001398">
        <property type="entry name" value="Macrophage_inhib_fac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014347">
        <property type="entry name" value="Tautomerase/MIF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11954">
        <property type="entry name" value="D-DOPACHROME DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11954:SF25">
        <property type="entry name" value="LS1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01187">
        <property type="entry name" value="MIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55331">
        <property type="entry name" value="Tautomerase/MIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9694068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="112" mass="12113" checksum="F1DA23808F213881" modified="2024-01-24" version="1">MPCVDISTNVNLEGVDADPIFSDVTKAVASIIGKPENYVMVLLKGSVAISFEGNKEPAAFAEIISMGGINSEVKRKLIATIGTILQTKLSIPRTRFFLKVYDTTMAHKIAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZTF8</accession>
    <name>A0AA38ZTF8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Methyltransferase type 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_009858</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZTF8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057192">
        <property type="entry name" value="DUF7870"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44843">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44843:SF2">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25276">
        <property type="entry name" value="DUF7870"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="1">
        <location>
            <begin position="146"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7870" evidence="2">
        <location>
            <begin position="280"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7870" evidence="2">
        <location>
            <begin position="416"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9694079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="511" mass="57762" checksum="EBDB36D3A327099D" modified="2024-01-24" version="1">MEPTAGKPSFMRNMMVRVLLFGVLVIGVRFAYVVTIRGESCDLGDFCFFSLPDNFNLPGAGKIGASAIVVKDAARSTFAAPSRVDLWTSKGWRKAVQFYSSVFQDLIVDGYLTPNSKSLCVETSAGQDVFALREIGVVDAIGTAKKKSPPLVVSALPSRQPFDDDTFDFVFSGGGAFDKVMRPLDFASEIARTLKPEGFLVVHTKTKDEYSFNSFVDLFNCCTVVKTRELDGMDSSMPYIREIVLKKEPGIISHGTPKSHSNSVNKCSVPGHKKELFRKAEPLITEEPLKPWITLKRNIKNIKYLSSMADISFKQRYVYVDVGARSYGSSIGSWFRKQYPKQNKTFEVYAIEADKTFHDEYKLKKRVTLLPYAAWVRNETLFFEINQDPGHKDVEKGRGMGRIQPGQSSASSDAVVDQIEGFDFANWLKNSVTERDFVVMKMDVEGTEFDLIPRLFETGAICLIDEIFLECHYNRWQRCCPGERSSKYQKTYDQCLDLFSNLRNIFELDVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZTS6</accession>
    <name>A0AA38ZTS6_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_010577</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Plays an important role in the elongation step of protein synthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">P1 and P2 exist as dimers at the large ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic ribosomal protein P1/P2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZTS6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002182">
        <property type="term" value="P:cytoplasmic translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05833">
        <property type="entry name" value="Ribosomal_P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1410:FF:000002">
        <property type="entry name" value="60S acidic ribosomal protein P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01478">
        <property type="entry name" value="Ribosomal_L12_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038716">
        <property type="entry name" value="P1/P2_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027534">
        <property type="entry name" value="Ribosomal_P1/P2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044076">
        <property type="entry name" value="Ribosomal_P2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21141">
        <property type="entry name" value="60S ACIDIC RIBOSOMAL PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21141:SF115">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN P2W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00428">
        <property type="entry name" value="Ribosomal_60s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="127"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="137"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9695148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="160" mass="16511" checksum="4C9494F9ECB649E0" modified="2024-01-24" version="1">MDQKPSPSSLCILISPTSQLRQLHPVVSLSLYLSISASPNLLFRAEMKVIAAYLLAVLGGNTCPSANDLKDILGSVGAEADDDRIELLLSEVKGKDITELIASGREKLASVPSGGGVAVAATAVGGGSGGGAPAAPEPKKEEKVEEKEESDDDMGFSLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZVE5</accession>
    <name>A0AA38ZVE5_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_010503</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZVE5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043424">
        <property type="entry name" value="BLT-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31071">
        <property type="entry name" value="GB|AAF24581.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31071:SF7">
        <property type="entry name" value="OS04G0382800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="45"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="501"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="606"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="337"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="47"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="105"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="125"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="150"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="506"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="523"/>
            <end position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9695038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="658" mass="74225" checksum="B65D96045309219A" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZVV1</accession>
    <name>A0AA38ZVV1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Disease resistance RPP13-like protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_008408</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057135">
        <property type="entry name" value="At4g27190-like_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056789">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766:SF40">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766">
        <property type="entry name" value="PLANT BROAD-SPECTRUM MILDEW RESISTANCE PROTEIN RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25019">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23247">
        <property type="entry name" value="LRR_RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0611">Plant defense</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="8">
        <location>
            <begin position="6"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="7">
        <location>
            <begin position="177"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="10">
        <location>
            <begin position="435"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="domain" description="R13L1/DRL21-like LRR repeat region" evidence="11">
        <location>
            <begin position="698"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein At4g27190-like leucine-rich repeats" evidence="9">
        <location>
            <begin position="1111"/>
            <end position="1254"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="120"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF25019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9696152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1671" mass="189889" checksum="8B595D8EA1F605CA" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZW70</accession>
    <name>A0AA38ZW70_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_008290</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9695952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZW70"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009873">
        <property type="term" value="P:ethylene-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000001">
        <property type="entry name" value="Ethylene-responsive transcription factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31677">
        <property type="entry name" value="AP2 DOMAIN CLASS TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31677:SF157">
        <property type="entry name" value="AP2_ERF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00367">
        <property type="entry name" value="ETHRSPELEMNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0936">Ethylene signaling pathway</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="8">
        <location>
            <begin position="24"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9695952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="168" mass="18542" checksum="9B65429561C00208" modified="2024-01-24" version="1">MAVEPLNRRSQPSGSGGAVTKEVHFRGVRKRPWGRFAAEIRDPWKKTRKWLGTFDTAEEAARAYDDAARSLRGPKAKTNFGDGGLSFASAEAPVAGASELFGKGLQQHWLQPSLTGGGDSVSVPSRSEYTGYRLDAVDLMVSEEEKKMRQEKEKKPFLFDLNLPAPLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZWH4</accession>
    <name>A0AA38ZWH4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">histidine kinase</fullName>
            <ecNumber evidence="5">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">PVL29_008675</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="24">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ethylene receptor family.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="5"/>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZWH4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051740">
        <property type="term" value="F:ethylene binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038199">
        <property type="term" value="F:ethylene receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010105">
        <property type="term" value="P:negative regulation of ethylene-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.130:FF:000004">
        <property type="entry name" value="Ethylene receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000030">
        <property type="entry name" value="Ethylene receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.40:FF:000026">
        <property type="entry name" value="Ethylene response sensor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058544">
        <property type="entry name" value="ETR1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24423:SF625">
        <property type="entry name" value="ETHYLENE RESPONSE SENSOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24423">
        <property type="entry name" value="TWO-COMPONENT SENSOR HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25487">
        <property type="entry name" value="ETR1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01590">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55781">
        <property type="entry name" value="GAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0186">Copper</keyword>
    <keyword evidence="19" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11" id="KW-0936">Ethylene signaling pathway</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="18 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 22" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="17" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="23"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="56"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="83"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="23">
        <location>
            <begin position="351"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="593"/>
            <end position="614"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KAJ9696573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="637" mass="71158" checksum="AF9A962B11F11D71" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZXT8</accession>
    <name>A0AA38ZXT8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cyclin-dependent kinase inhibitor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_009134</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the CDI family. ICK/KRP subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZXT8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004861">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.365.10">
        <property type="entry name" value="p27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003175">
        <property type="entry name" value="CDI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044898">
        <property type="entry name" value="CDI_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044275">
        <property type="entry name" value="KRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46776">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE INHIBITOR 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02234">
        <property type="entry name" value="CDI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017811">
        <property type="entry name" value="CDK_inhib_pln"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 5" id="KW-0649">Protein kinase inhibitor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-dependent kinase inhibitor" evidence="7">
        <location>
            <begin position="172"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="36"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="86"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="147"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR017811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9697216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="218" mass="24155" checksum="AEB4D8A5EEB7DCB7" modified="2024-01-24" version="1">MGKYMKKAKITGDVTVMEVSSSLGVRTRAKTLALQRLQKTTHQEAPKPDTSYLELRSRRLEKPPLLCEPNKKQSPNPKASSRVELGSVNSNSVGSVPPGEKINAVETEDVGVEASYGENVLEFEGRDRSTRESTPCSLIRDPDTIATPGSTTRPTSSNATTRRVRSNVQGDIPTAAELEEFFTHAEEHAQKEFMDKYNFDIVNDSPFPGRFEWVQVSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A058</accession>
    <name>A0AA39A058_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">3-ketoacyl-CoA synthase</fullName>
            <ecNumber evidence="6">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_007410</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a very-long-chain acyl-CoA + malonyl-CoA + H(+) = a very-long-chain 3-oxoacyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:32727"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:90725"/>
            <dbReference type="ChEBI" id="CHEBI:90736"/>
            <dbReference type="EC" id="2.3.1.199"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the thiolase-like superfamily. Chalcone/stilbene synthases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="6"/>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A058"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009922">
        <property type="term" value="F:fatty acid elongase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00831">
        <property type="entry name" value="CHS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012392">
        <property type="entry name" value="3-ktacl-CoA_syn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013747">
        <property type="entry name" value="ACP_syn_III_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013601">
        <property type="entry name" value="FAE1_typ3_polyketide_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31561">
        <property type="entry name" value="3-KETOACYL-COA SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08541">
        <property type="entry name" value="ACP_syn_III_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08392">
        <property type="entry name" value="FAE1_CUT1_RppA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036417">
        <property type="entry name" value="3-ktacl-CoA_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="160"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="FAE" evidence="9">
        <location>
            <begin position="26"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-ketoacyl-[acyl-carrier-protein] synthase III C-terminal" evidence="10">
        <location>
            <begin position="331"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="247"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="333"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="337"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="366"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR036417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR036417-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9698314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="470" mass="53373" checksum="FC0E8C268D49F803" modified="2024-01-24" version="1">MEAMAYLFLLPVFYLFFLLWKFIDGKRDQECYILDYQCYKPTDDRKLSTEFCGDMMRRNKSLGLQEYKFLLKAIVNSGIGEETYGPRIVFSGNETHPSVLDGISEMDEFFNDSIEKLLDRVGVSPSEIDALVVNVSMITTAPSLAGRIINRYKMRENIKAFNLTGMGCSASLISINIVQNLFKSYKNMYALVVTSESLSPNWYTGNDRSMILSNCLFRSGGCAVLLTNKRALRHRAMLKLRCLVRTHHGAKDEAFGCCIQKEDDQGRLGFFLNKSLPKAATRAFIDNLRVISPKILPVRELLRFMVASLVRKMHQNSKGGGKVVINFKTGVDHFCIHTGGKAVIDGIGKSLELTEYDLEPARMTLHRFGNTSASSLWYVLAYMDEKKRLKKGDRVLMISFGAGFKCNSCMWEVARDLEGGNVWKDCIDSYPPESLSNPFMEKYGWINQEDENTFEEKLKEIDEAKALKLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2025-04-02" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A0H6</accession>
    <name>A0AA39A0H6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_007581</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16449">
        <property type="entry name" value="RING-HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37393">
        <property type="entry name" value="AT-RICH INTERACTIVE DOMAIN-CONTAINING PROTEIN 1A-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37393:SF1">
        <property type="entry name" value="AT-RICH INTERACTIVE DOMAIN-CONTAINING PROTEIN 1A-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="20"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="253"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="280"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="622"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="718"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="950"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1030"/>
            <end position="1257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1550"/>
            <end position="1575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1645"/>
            <end position="1680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="280"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="322"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="366"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="408"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="424"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="442"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="460"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="471"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="505"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="532"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="629"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="725"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="774"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="799"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="964"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1653"/>
            <end position="1665"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9698582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1680" mass="183368" checksum="FEC3227388422483" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A315</accession>
    <name>A0AA39A315_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pectinesterase inhibitor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_005622</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the PMEI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9699844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A315"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004857">
        <property type="term" value="F:enzyme inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15796">
        <property type="entry name" value="CIF_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.40:FF:000009">
        <property type="entry name" value="Invertase/pectin methylesterase inhibitor family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.40">
        <property type="entry name" value="Invertase/pectin methylesterase inhibitor family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034087">
        <property type="entry name" value="C/VIF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035513">
        <property type="entry name" value="Invertase/methylesterase_inhib"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006501">
        <property type="entry name" value="Pectinesterase_inhib_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01614">
        <property type="entry name" value="PME_inhib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35357:SF8">
        <property type="entry name" value="OS01G0111000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35357">
        <property type="entry name" value="OS02G0537100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04043">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00856">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101148">
        <property type="entry name" value="Plant invertase/pectin methylesterase inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041260613" description="Pectinesterase inhibitor domain-containing protein" evidence="4">
        <location>
            <begin position="24"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="Pectinesterase inhibitor" evidence="5">
        <location>
            <begin position="21"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9699844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="169" mass="17974" checksum="C7C8DC6E7042F427" modified="2024-01-24" version="1">MRSSVFLLCVVGLSLTQIQFSSADLVDQTCKQTPNYDLCVKTLRADPRSSKADVTGLALVMVDTIKAKATATLDRINGLLGTTPDPKTKAALSHFVELYDNAVLKADIPSAIEALKKGNPKFAEQGINDAANEADSCDRAFGGASPITSFNRNVSDLSRVASAIIRLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A633</accession>
    <name>A0AA39A633_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_006051</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A633"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11393">
        <property type="entry name" value="bHLH_AtbHLH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044658">
        <property type="entry name" value="bHLH92/bHLH041-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045239">
        <property type="entry name" value="bHLH95_bHLH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46665">
        <property type="entry name" value="TRANSCRIPTION FACTOR BHLH041-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46665:SF6">
        <property type="entry name" value="TRANSCRIPTION FACTOR BHLH92"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="214"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="BHLH" evidence="8">
        <location>
            <begin position="84"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="123"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9700559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="244" mass="28432" checksum="825BBF69B71282AD" modified="2024-01-24" version="1">MMDHFFDYGSQHEMFWFEYPPMNSNPPTQEFHSENSAFAPYRSQPIPGDGGVGGSNRRNMNRRMMELLRGNWSAIRYAGQPDRGRSYRHMLRERARRENQKQSYLALHSLLPHGTKSDKNSIIEMANHEIQKLQSSKEELKRRNQELKAKLREEEMMLNIGLRVGNPSSGIDSMEEVLKCLKNMDLKTTAIHSQFSPQQLSAVVKIQTQVLLSFNFPLFSFLFFSFLPFIFYSPSPQIEKAFKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A6C8</accession>
    <name>A0AA39A6C8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein ENDOSPERM DEFECTIVE 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_006874</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the QWRF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A6C8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005880">
        <property type="term" value="C:nuclear microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051225">
        <property type="term" value="P:spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007573">
        <property type="entry name" value="QWRF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31807">
        <property type="entry name" value="AUGMIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31807:SF6">
        <property type="entry name" value="PROTEIN ENDOSPERM DEFECTIVE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04484">
        <property type="entry name" value="QWRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="218"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="39"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="72"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="218"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="238"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9701686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="620" mass="67701" checksum="19EC2F6AE82AC599" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A780</accession>
    <name>A0AA39A780_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_004102</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A780"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033612">
        <property type="term" value="F:receptor serine/threonine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000221">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase PXL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001424">
        <property type="entry name" value="Protein kinase superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050647">
        <property type="entry name" value="Plant_LRR-RLKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056:SF29">
        <property type="entry name" value="RECEPTOR-LIKE PROTEIN KINASE HSL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="405"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="467"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="496"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9702197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="787" mass="87679" checksum="91A336810C4F0C9F" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A8H2</accession>
    <name>A0AA39A8H2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">ADP-ribosylation factor 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_004525</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">GTP-binding protein involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A8H2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04151">
        <property type="entry name" value="Arl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000510">
        <property type="entry name" value="ADP-ribosylation factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 12 14" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0519">Myristate</keyword>
    <keyword evidence="5 12 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="24"/>
            <end position="31"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="126"/>
            <end position="129"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00053326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9702826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="181" mass="20338" checksum="ADC302585D1E11B0" modified="2024-01-24" version="1">MGILFTRMFSSVFGNREARILVLGLDNAGKTTILYRLQMGEVVSTIPTIGFNVETVQYNNIKFQVWDLGGQTSIRPYWRCYFPNTQAIIYVVDSSDTDRIVIAKDEFHAILEEEELKGAIVLIFANKQDLPGALDDAALTEALELHKIKNRQWAIFKTCAIKGEGLYEGLDWLSNTLKSGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ABV6</accession>
    <name>A0AA39ABV6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">AT4G36440-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_005065</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ABV6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35752">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35752:SF1">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041216436" description="AT4G36440-like protein" evidence="2">
        <location>
            <begin position="24"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="317"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9703573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="410" mass="44826" checksum="B7471133B7574F30" modified="2024-01-24" version="1">MLSLGNCLDGHSLYCLLITTVTAVILQVPGASSIVAPSSNCYALDNSSHIFDFSSWLGQPFEYDGKDSDLVVRFCKDVESRSQTGYVDFGRFDKLNHFVAGSGHINFVQEFYNGDLLNCERTHDKMGRTAQVNIICGSCSNGQCTGELGCICNVTYESTCRVLVELAIPCKEQGVRVFQGFTVGFHPRSWEIVYNGMTQLGFDKSHHAFSFGTEQTYVALYMTAIASLSTLVQKPTVKIFPEHGLKVGLSGSGATGRPPTTLSPTMLVVDWSCEKAHDTPYEVDITIPVEGYEPIQFTLTKMCEYPQGKGGGTTRGWAIFGVASCIFIVLSTLFCCGGFVYKTRVERQRGLDALPGMTILSACLEAVSGGSHYSRAEDTNSAFINQASWERQPDAAQGTWKTSERKYGSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AD22</accession>
    <name>A0AA39AD22_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_003427</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AD22"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05381">
        <property type="entry name" value="CAP_PR-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.33.10:FF:000004">
        <property type="entry name" value="CAP, cysteine-rich secretory protein, antigen 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018244">
        <property type="entry name" value="Allrgn_V5/Tpx1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001283">
        <property type="entry name" value="CRISP-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10334">
        <property type="entry name" value="CYSTEINE-RICH SECRETORY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00837">
        <property type="entry name" value="V5TPXLIKE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01009">
        <property type="entry name" value="CRISP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041324508" description="SCP domain-containing protein" evidence="1">
        <location>
            <begin position="25"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="SCP" evidence="2">
        <location>
            <begin position="26"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9705375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="163" mass="18120" checksum="4F73ED4CE0C559AC" modified="2024-01-24" version="1">MDSSVLETLVIACVFFMLPSIFLAQNSPDDFVNAHNDVRATVGVQCLVWNTTLQEYAQSYANNRSSDCLLRLSGAPDYGENVFIGTPDNYTARDAVNTWAAERQYYNYDTNTCMMGQVCGHYTQLVWNTTTSVGCARVPCVNGSVFITCNYYLAGNVIGQRPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AE97</accession>
    <name>A0AA39AE97_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_003606</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AE97"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9705619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="64" mass="6289" checksum="5345BA5F3D10327F" modified="2024-01-24" version="1">MSSSSSSSQAIRGTTSSTLMSSLVLANNRSIPVSGSVLAAKEDKNAEEAEMEGEGGADGGVGAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AFU0</accession>
    <name>A0AA39AFU0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">transaldolase</fullName>
            <ecNumber evidence="3">2.2.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_002014</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; pentose phosphate pathway; D-glyceraldehyde 3-phosphate and beta-D-fructose 6-phosphate from D-ribose 5-phosphate and D-xylulose 5-phosphate (non-oxidative stage): step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transaldolase family. Type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.2" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AFU0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004801">
        <property type="term" value="F:transaldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00957">
        <property type="entry name" value="Transaldolase_TalAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000163">
        <property type="entry name" value="Transaldolase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001585">
        <property type="entry name" value="TAL/FSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004730">
        <property type="entry name" value="Transaldolase_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10683">
        <property type="entry name" value="TRANSALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10683:SF18">
        <property type="entry name" value="TRANSALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00923">
        <property type="entry name" value="TAL_FSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0570">Pentose shunt</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0704">Schiff base</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9706836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="396" mass="43515" checksum="FF873C10E218FAE6" modified="2024-01-24" version="1">MSLLLQSPPSSALSSSSRQEGRLRFLARSATPRFALSFQRSFPQIRASANFSSSLDKGLSTEFDAISDLSEIVPDTVIFDDFERFPPTAATVSSSLLLGICSLPDTIFRSAVETALADSVCYGFESPDARMSCFFNKALVNVGSDLVKLVPGRVSTEVDARLAYDTHGIIRKVHDLLTLYNEIDVPPERLLFKIPSTWQGIEASRLLESEGIQTHLTFVYSFCQAAAAAQAGASVIQIFVGRVRDWARNHSGDPEIEAALKRGEDPGLALVTKAYNYIHKHGYKSKLMAAAVRNKQDLFSLLGVDYIIAPLKVLESLKESITSPDEKYSFVRRLSPNSAASYSFSEEELVKWDQLNFASSMGPAAVELLAAGLEGYATQAKRVEELFEKIWPPPNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AG14</accession>
    <name>A0AA39AG14_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-CH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_001768</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AG14"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46214:SF16">
        <property type="entry name" value="OS10G0481450 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46214">
        <property type="entry name" value="ZINC FINGER, RING-CH-TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="112"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="RING-CH-type" evidence="5">
        <location>
            <begin position="35"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9706422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="182" mass="19755" checksum="6D0978F4509D62D5" modified="2024-01-24" version="1">MDITLTTILVSNGDSRVQVVVSDGDQLSPKKPHLSRTPALTMNAVCHADMEEDLIELGCHCRGWLAKAHRTYIDAWFRTRGSNKCEICKQVVANVPPPESLPSGGFRPVCEALTILILGLLVDVLVSIVFGVFTVNIIIATRIMSVLGFGTALCIAGRFSQSSRRIMQGAETNMNLGYLPAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AIG6</accession>
    <name>A0AA39AIG6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DDE Tnp4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_002637</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HARBI1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AIG6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058353">
        <property type="entry name" value="DUF8040"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045249">
        <property type="entry name" value="HARBI1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930:SF268">
        <property type="entry name" value="NUCLEASE HARBI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26138">
        <property type="entry name" value="DUF8040"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF8040" evidence="9">
        <location>
            <begin position="33"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="DDE Tnp4" evidence="8">
        <location>
            <begin position="131"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF26138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9707682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="205" mass="24172" checksum="D8991FF18BA07F9A" modified="2024-01-24" version="1">MYVAYTAILVLVYHHSNYLDRSISNEGDYERHALMERLTLMDNEYCHKQLRMGKDAFARLVNILRGIGHLRNSAHSNVEEQVAKFLHIVGQNLRNTTIKFYFKHYSETISHHLHQVLRAIISLDDDCIRVIDGSHFRVKVSNDVVQRYRRRKYYPTQNVLVTCSFDLKFTYVLPSWEGSASDLRILDYALTRDFDKLIVPQGDYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AK76</accession>
    <name>A0AA39AK76_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_000907</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AK76"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000736">
        <property type="entry name" value="Putative serine/threonine-protein kinase At1g18390 family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46008">
        <property type="entry name" value="LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-LIKE 1.4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46008:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041264723" description="Protein kinase domain-containing protein" evidence="5">
        <location>
            <begin position="36"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="265"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="331"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="359"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9709170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="640" mass="70559" checksum="0B49FCED4C360AC4" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AN34</accession>
    <name>A0AA39AN34_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein TIFY</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Jasmonate ZIM domain-containing protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_001442</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Repressor of jasmonate responses.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="2">The jas domain is required for interaction with COI1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the TIFY/JAZ family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AN34"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031347">
        <property type="term" value="P:regulation of defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000022">
        <property type="term" value="P:regulation of jasmonic acid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009611">
        <property type="term" value="P:response to wounding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018467">
        <property type="entry name" value="CCT_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040390">
        <property type="entry name" value="TIFY/JAZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010399">
        <property type="entry name" value="Tify_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33077">
        <property type="entry name" value="PROTEIN TIFY 4A-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33077:SF5">
        <property type="entry name" value="PROTEIN TIFY 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09425">
        <property type="entry name" value="Jas_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06200">
        <property type="entry name" value="tify"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00979">
        <property type="entry name" value="TIFY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51320">
        <property type="entry name" value="TIFY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1184">Jasmonic acid signaling pathway</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tify" evidence="4">
        <location>
            <begin position="100"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="59"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="70"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU369065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9709962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="211" mass="23534" checksum="30A0DD526C7DAE21" modified="2024-01-24" version="1">MSRAAMELDFFGMEKDGAAAKSQFQRFLDRRRSFRGMQSAISKINPDLLKTVIASGSVSQAPKNNPPFASRNSLSVPSTPKSDQNYLPALPVYSPVFRPVSAETTPITIFYNGTVSVFDVSPEQIESIMKLALDGSTKTVEPADSKLAIPPNEEQQLLETLNGDLPLARRKSLHRFLEKRKERLTSVSPYPPQIRYAVSDQEKITEELFRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ARF3</accession>
    <name>A0AA39ARF3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">PVL29_001317</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="24">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ARF3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000467">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000722">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000101">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001023">
        <property type="entry name" value="Os07g0541700 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046959">
        <property type="entry name" value="PRK1-6/SRF4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48007">
        <property type="entry name" value="LEUCINE-RICH REPEAT RECEPTOR-LIKE PROTEIN KINASE PXC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48007:SF36">
        <property type="entry name" value="RECEPTOR PROTEIN KINASE-LIKE PROTEIN ZAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="15 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9 22" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041290142" description="non-specific serine/threonine protein kinase" evidence="22">
        <location>
            <begin position="23"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="315"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="410"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="284"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="289"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KAJ9709799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="709" mass="77145" checksum="942411C1048A29DA" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D620</accession>
    <name>A0AA39D620_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_025611</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ubiquitin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D620"/>
    <dbReference type="SMR" id="A0AA39D620"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01803">
        <property type="entry name" value="Ubl_ubiquitin"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000016">
        <property type="entry name" value="Polyubiquitin 3"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019954">
        <property type="entry name" value="Ubiquitin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019956">
        <property type="entry name" value="Ubiquitin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050158">
        <property type="entry name" value="Ubiquitin_ubiquitin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10666">
        <property type="entry name" value="UBIQUITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00348">
        <property type="entry name" value="UBIQUITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00299">
        <property type="entry name" value="UBIQUITIN_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="7">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="7">
        <location>
            <begin position="77"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="7">
        <location>
            <begin position="153"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="7">
        <location>
            <begin position="229"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="7">
        <location>
            <begin position="305"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="7">
        <location>
            <begin position="381"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="7">
        <location>
            <begin position="457"/>
            <end position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9672039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="533" mass="59713" checksum="C09D194FC2E77CE8" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D7C6</accession>
    <name>A0AA39D7C6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="30">Acetyl-CoA carboxylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="28" type="ORF">PVL29_023372</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="28 29" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="28">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>N(6)-biotinyl-L-lysyl-[protein] + hydrogencarbonate + ATP = N(6)-carboxybiotinyl-L-lysyl-[protein] + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13501"/>
            <dbReference type="Rhea" id="RHEA-COMP:10505"/>
            <dbReference type="Rhea" id="RHEA-COMP:10506"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83144"/>
            <dbReference type="ChEBI" id="CHEBI:83145"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.14"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>hydrogencarbonate + acetyl-CoA + ATP = malonyl-CoA + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11308"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.4.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>biotin</name>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="28">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003989">
        <property type="term" value="F:acetyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004075">
        <property type="term" value="F:biotin carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06850">
        <property type="entry name" value="biotinyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000005">
        <property type="entry name" value="Acetyl-CoA carboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000043">
        <property type="entry name" value="acetyl-CoA carboxylase 1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000003">
        <property type="entry name" value="acetyl-CoA carboxylase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000005">
        <property type="entry name" value="acetyl-CoA carboxylase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000010">
        <property type="entry name" value="acetyl-CoA carboxylase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.460.10">
        <property type="entry name" value="Biotin dependent carboxylase carboxyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1770.10">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049076">
        <property type="entry name" value="ACCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049074">
        <property type="entry name" value="ACCA_BT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034733">
        <property type="entry name" value="AcCoA_carboxyl_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013537">
        <property type="entry name" value="AcCoA_COase_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005481">
        <property type="entry name" value="BC-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001882">
        <property type="entry name" value="Biotin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011764">
        <property type="entry name" value="Biotin_carboxylation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005482">
        <property type="entry name" value="Biotin_COase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011763">
        <property type="entry name" value="COA_CT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011762">
        <property type="entry name" value="COA_CT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45728:SF3">
        <property type="entry name" value="ACETYL-COA CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45728">
        <property type="entry name" value="ACETYL-COA CARBOXYLASE, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08326">
        <property type="entry name" value="ACC_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21385">
        <property type="entry name" value="ACCA_BT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02785">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00289">
        <property type="entry name" value="Biotin_carb_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01039">
        <property type="entry name" value="Carboxyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00878">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50979">
        <property type="entry name" value="BC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00188">
        <property type="entry name" value="BIOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50989">
        <property type="entry name" value="COA_CT_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50980">
        <property type="entry name" value="COA_CT_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="13 22" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="18" id="KW-0092">Biotin</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="9" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="15" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="17" id="KW-0464">Manganese</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="11 22" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Biotin carboxylation" evidence="25">
        <location>
            <begin position="46"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="24">
        <location>
            <begin position="199"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoyl-binding" evidence="23">
        <location>
            <begin position="680"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="domain" description="CoA carboxyltransferase N-terminal" evidence="26">
        <location>
            <begin position="1503"/>
            <end position="1842"/>
        </location>
    </feature>
    <feature type="domain" description="CoA carboxyltransferase C-terminal" evidence="27">
        <location>
            <begin position="1846"/>
            <end position="2161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00048600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="EMBL" id="KAJ9673781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="30">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2266" mass="252153" checksum="01584BBC7A393AD6" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D7Z8</accession>
    <name>A0AA39D7Z8_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_023531</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TTC36 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D7Z8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038906">
        <property type="entry name" value="TTC36"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21405">
        <property type="entry name" value="CDNA SEQUENCE BC021608"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21405:SF0">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="172"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9674034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="218" mass="23591" checksum="88A2B828A8DB5111" modified="2024-01-24" version="1">MGTEMVNDIAIQVGMILFTVLMFLYTSDIPGKALAKLRLWYNQTNLEAKRHFVLGAQRLAQARSPDTSPSDVISLAKTAAEEADKALSLNPKDAAAHILKALALDLQGFRLSALDSLDAALSPLAVKSLTDQERGDALIKRAQLKIGLDKVGRVDSAVADLVEAVSVGSEKDKAYCLLGECYEMKEMKEEATKAYQEAIKIQPNSALARDALDRLTTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D8Y2</accession>
    <name>A0AA39D8Y2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Proteasome subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_023251</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the proteasome, a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="function">
        <text evidence="5">Non-catalytic component of the proteasome, a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the 20S core complex of the 26S proteasome. The 26S proteasome is composed of a core protease (CP), known as the 20S proteasome, capped at one or both ends by the 19S regulatory particle (RP/PA700). The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the proteasome complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase T1B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D8Y2"/>
    <dbReference type="SMR" id="A0AA39D8Y2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03759">
        <property type="entry name" value="proteasome_beta_type_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000032">
        <property type="entry name" value="Proteasome subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033811">
        <property type="entry name" value="Proteasome_beta_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF10">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA TYPE-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3 6" id="KW-0647">Proteasome</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9673567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="204" mass="22920" checksum="6D9094E70F287B16" modified="2024-01-24" version="1">MSIFEYNGSALIAMVGKNCFAIASDRRFGVQLQTVATDFQKIYRIHDRVFLGLSGLATDAQTLYQRLVFRHKLYQLREERDMKPETFASLVSALLYEKRFGPYFCQPVIAGLGDEDKPFICTMDSIGAKELAKDFVVAGTASESLYGACEAMFKPDMEPEELFEIISQALLSSVDRDCLSGWGGHVYVVTPTEVKERILKGRMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DA43</accession>
    <name>A0AA39DA43_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF538 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_022461</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DA43"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.240.10">
        <property type="entry name" value="At5g01610-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036758">
        <property type="entry name" value="At5g01610-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007493">
        <property type="entry name" value="DUF538"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31676:SF74">
        <property type="entry name" value="DUF538 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31676">
        <property type="entry name" value="T31J12.3 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04398">
        <property type="entry name" value="DUF538"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141562">
        <property type="entry name" value="At5g01610-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9677483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="173" mass="19701" checksum="5FA31CA8156056ED" modified="2024-01-24" version="1">MEKALTKVGSFWISKKAKEEISNITEDLSTFSNTVEEKARWVFNKLKGKPAKALPDLLREHNLPPGLFPRNITCYEFDESRSKLIVYLPSACEVSFKDSSIIRYATRVKGTLARGKLTGIEGMKTKVLVWVKVTIVSVETYKSDKVWFTAGVKKSRPKDAYEMTRDAVKIEEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DBF4</accession>
    <name>A0AA39DBF4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Alpha-N-acetylglucosaminidase</fullName>
            <ecNumber evidence="7">3.2.1.50</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_022556</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>Hydrolysis of terminal non-reducing N-acetyl-D-glucosamine residues in N-acetyl-alpha-D-glucosaminides.</text>
            <dbReference type="EC" id="3.2.1.50"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the glycosyl hydrolase 89 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.50" evidence="7"/>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DBF4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004561">
        <property type="term" value="F:alpha-N-acetylglucosaminidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000107">
        <property type="entry name" value="Alpha-N-acetylglucosaminidase family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.379.10">
        <property type="entry name" value="Chitobiase/beta-hexosaminidase domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.670">
        <property type="entry name" value="N-acetyl-b-d-glucoasminidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029018">
        <property type="entry name" value="Hex-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007781">
        <property type="entry name" value="NAGLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024732">
        <property type="entry name" value="NAGLU_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024240">
        <property type="entry name" value="NAGLU_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024733">
        <property type="entry name" value="NAGLU_tim-barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12872">
        <property type="entry name" value="ALPHA-N-ACETYLGLUCOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12872:SF3">
        <property type="entry name" value="ALPHA-N-ACETYLGLUCOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05089">
        <property type="entry name" value="NAGLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12972">
        <property type="entry name" value="NAGLU_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12971">
        <property type="entry name" value="NAGLU_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041276086" description="Alpha-N-acetylglucosaminidase" evidence="9">
        <location>
            <begin position="24"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-N-acetylglucosaminidase N-terminal" evidence="11">
        <location>
            <begin position="50"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-N-acetylglucosaminidase tim-barrel" evidence="10">
        <location>
            <begin position="162"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-N-acetylglucosaminidase C-terminal" evidence="12">
        <location>
            <begin position="506"/>
            <end position="803"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00052030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00060996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00066522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00072202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF12971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF12972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9677634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="813" mass="92414" checksum="E1495F770EE01AEC" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2025-04-02" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DCI6</accession>
    <name>A0AA39DCI6_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_022322</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.790">
        <property type="entry name" value="Surp module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000061">
        <property type="entry name" value="Surp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035967">
        <property type="entry name" value="SWAP/Surp_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052650">
        <property type="entry name" value="Zinc_finger_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36886:SF7">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36886">
        <property type="entry name" value="PROTEIN FRIGIDA-ESSENTIAL 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01805">
        <property type="entry name" value="Surp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109905">
        <property type="entry name" value="Surp module (SWAP domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50128">
        <property type="entry name" value="SURP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SURP motif" evidence="5">
        <location>
            <begin position="306"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="4">
        <location>
            <begin position="771"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="2">
        <location>
            <begin position="771"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="186"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="386"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="554"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="594"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="793"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="840"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="983"/>
            <end position="1091"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1190"/>
            <end position="1239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1411"/>
            <end position="1437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="16"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="67"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="89"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="108"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="128"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="203"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="260"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="650"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="681"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="704"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="758"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="794"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="846"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="864"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="995"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1023"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1190"/>
            <end position="1200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1416"/>
            <end position="1429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9677257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1640" mass="177452" checksum="56471DD2080DBD39" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DDD4</accession>
    <name>A0AA39DDD4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">S-type anion channel SLAH2-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_021516</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SLAC1 S-type anion channel family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DDD4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008308">
        <property type="term" value="F:voltage-gated monoatomic anion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006873">
        <property type="term" value="P:intracellular monoatomic ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09323">
        <property type="entry name" value="TDT_SLAC1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.150">
        <property type="entry name" value="Voltage-dependent anion channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030183">
        <property type="entry name" value="SLAC/SLAH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004695">
        <property type="entry name" value="SLAC1/Mae1/Ssu1/TehA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038665">
        <property type="entry name" value="Voltage-dep_anion_channel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31269">
        <property type="entry name" value="S-TYPE ANION CHANNEL SLAH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31269:SF2">
        <property type="entry name" value="S-TYPE ANION CHANNEL SLAH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03595">
        <property type="entry name" value="SLAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="251"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="292"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="332"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="358"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="396"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="419"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="449"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="480"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="506"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="537"/>
            <end position="563"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9679614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="627" mass="70332" checksum="B360BD9AFA290C11" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DE29</accession>
    <name>A0AA39DE29_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Peptidase metallopeptidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_019848</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="11">Can bind about 5 Ca(2+) ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M10A family. Matrix metalloproteinases (MMPs) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DE29"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030574">
        <property type="term" value="P:collagen catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04278">
        <property type="entry name" value="ZnMc_MMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000018">
        <property type="entry name" value="Metalloendoproteinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033739">
        <property type="entry name" value="M10A_MMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001818">
        <property type="entry name" value="Pept_M10_metallopeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021190">
        <property type="entry name" value="Pept_M10A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002477">
        <property type="entry name" value="Peptidoglycan-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036365">
        <property type="entry name" value="PGBD-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201">
        <property type="entry name" value="MATRIX METALLOPROTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201:SF321">
        <property type="entry name" value="METALLOENDOPROTEINASE 4-MMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00413">
        <property type="entry name" value="Peptidase_M10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01471">
        <property type="entry name" value="PG_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00138">
        <property type="entry name" value="MATRIXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47090">
        <property type="entry name" value="PGBD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <keyword evidence="6 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041451829" description="Peptidase metallopeptidase domain-containing protein" evidence="12">
        <location>
            <begin position="22"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase metallopeptidase" evidence="13">
        <location>
            <begin position="143"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="266"/>
        </location>
    </feature>
    <feature type="binding site" description="in inhibited form" evidence="11">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR621190-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR621190-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9680651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="353" mass="39563" checksum="286718ECF9661051" modified="2024-01-24" version="1">MFPLLTLFSLLFFLLFSPCQPGRPPPELAPSPETVVTVKNGSYTWHGFQRFLGARRGSRISGMAELKKYFNRFGYLGFQDGNVTDVFDTRLESAVAAYQVKLGLPVTGRLDSETLLQMMSPRCGIRDAMQPMHAAMHYVYFPGKPRWARPIPMTLTYAFSPENLIGYLSLEDIRRAFKRSFARWASVIPVSFTETDTYSFADIKIGFYRGDHGDGEPFDGVLGVLAHAFSPESGKFHLDAAETWAVDLESEKSMVAIDLESVVTHEIGHLLGLAHSPVKESVMYPTLKPRAKKTDLKLDDIEAVQALYGSNPNFKFASSMESDISSNQAIDFRVRSYRKLSILLPILVLYSSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DER2</accession>
    <name>A0AA39DER2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_020175</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SINA (Seven in absentia) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9681159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DER2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16571">
        <property type="entry name" value="RING-HC_SIAHs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000041">
        <property type="entry name" value="E3 ubiquitin-protein ligase SINAT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.210.10:FF:000027">
        <property type="entry name" value="Pectinesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.210.10">
        <property type="entry name" value="Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018121">
        <property type="entry name" value="7-in-absentia-prot_TRAF-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052088">
        <property type="entry name" value="E3_ubiquitin-ligase_SINA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049548">
        <property type="entry name" value="Sina-like_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008974">
        <property type="entry name" value="TRAF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013010">
        <property type="entry name" value="Znf_SIAH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10315">
        <property type="entry name" value="E3 UBIQUITIN PROTEIN LIGASE SIAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10315:SF80">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SINAT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21362">
        <property type="entry name" value="Sina_RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03145">
        <property type="entry name" value="Sina_TRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21361">
        <property type="entry name" value="Sina_ZnF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51081">
        <property type="entry name" value="ZF_SIAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="11">
        <location>
            <begin position="20"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="SIAH-type" evidence="12">
        <location>
            <begin position="73"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9681159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="270" mass="30532" checksum="3733C28C372FC782" modified="2024-01-24" version="1">MGYRVADLHSLTKFQELLKCPVCFNFMQSPIYQCHNGHTLCSSCKARVLNKCPSCRQQLGNIRCLALEKMAESLELHCKYEEFGCPEIIPYHTKLMHEDSCNFRPYSCPWSGCPCSAVGDIPLLVSHLTDYHKAVMLYDCKFKLEFLIEDLYKYQNYKWDVTIINCFDKHFCLHAEAFLIGSTPVYMAFLSLIGNQAEAGNYSYSLEIGGNGRKLTFEGIPRSIRESKRRSLESADSLIVLGGMAFSLGGETRMSMLGVTGRIRKAQIPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DFE2</accession>
    <name>A0AA39DFE2_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_019470</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DFE2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9680182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="171" mass="19236" checksum="5D434849191E1B40" modified="2024-01-24" version="1">MPIHFAIIFNFPGINLPQQQNRMGTTRFRHSLHSYPSLFEAHPIITKVAIAALQLFGLSFTLEEYFAHLSPTPAALLRTSMVFSGSLLVASLVSILSSDEWRPVIYVIYVFLSLGNYLYRLVRKGCGCVQGIMEQRHVTRRSAILPLTNMDTHHRVRTDTSRSTASASLDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DG34</accession>
    <name>A0AA39DG34_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Aldose 1-epimerase</fullName>
            <ecNumber evidence="5">5.1.3.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_019275</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>alpha-D-glucose = beta-D-glucose</text>
            <dbReference type="Rhea" id="RHEA:10264"/>
            <dbReference type="ChEBI" id="CHEBI:15903"/>
            <dbReference type="ChEBI" id="CHEBI:17925"/>
            <dbReference type="EC" id="5.1.3.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 5">Carbohydrate metabolism; hexose metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the aldose epimerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.3" evidence="5"/>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DG34"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004034">
        <property type="term" value="F:aldose 1-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033499">
        <property type="term" value="P:galactose catabolic process via UDP-galactose, Leloir pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09019">
        <property type="entry name" value="galactose_mutarotase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.98.10:FF:000008">
        <property type="entry name" value="Aldose 1-epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015443">
        <property type="entry name" value="Aldose_1-epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008183">
        <property type="entry name" value="Aldose_1/G6P_1-epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047215">
        <property type="entry name" value="Galactose_mutarotase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014718">
        <property type="entry name" value="GH-type_carb-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008277">
        <property type="entry name" value="PRK11055.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10091">
        <property type="entry name" value="ALDOSE-1-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10091:SF0">
        <property type="entry name" value="GALACTOSE MUTAROTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01263">
        <property type="entry name" value="Aldose_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005096">
        <property type="entry name" value="GALM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041226620" description="Aldose 1-epimerase" evidence="9">
        <location>
            <begin position="24"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="192"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="323"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="90"/>
            <end position="91"/>
        </location>
        <ligand>
            <name>beta-D-galactose</name>
            <dbReference type="ChEBI" id="CHEBI:27667"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="192"/>
            <end position="194"/>
        </location>
        <ligand>
            <name>beta-D-galactose</name>
            <dbReference type="ChEBI" id="CHEBI:27667"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>beta-D-galactose</name>
            <dbReference type="ChEBI" id="CHEBI:27667"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR005096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR005096-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR005096-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR005096-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9683633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="359" mass="38979" checksum="AD8ECA48002E3080" modified="2024-01-24" version="1">MASISVFVCFITLVFCFATGSVAKKEEGRIYELRKGDFSAKFTKWGASIVSVVLPDKHGKLADVVLGYDSAKEYKNDTTNFGAIVGRVANRIGGAQFTLNGTHYKLVANDGKNTLHGGPKGFGKVAWRVSKYKKGGHSPYIMFSYHSLDGEEGFPGDLIATVTYMLVGDYQLIVTMKAKALNKATPVNLAQHAYWNLGGHTSGDILSNEIQIFGSHITPVDKELIPTGKIVSVKGTPYDFLKPCTIGSNINKLPSGFDINYVLDDIAPNKVGLAAMVHHKKSGRMLELYTNAPGLQFYTANSLKDVKGKGGFVYQAHAALCLETQGFPDSVNHPNFPSQIVTPGKSYHHSMLFKFSIGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DHM9</accession>
    <name>A0AA39DHM9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_018441</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DHM9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057135">
        <property type="entry name" value="At4g27190-like_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050905">
        <property type="entry name" value="Plant_NBS-LRR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463:SF198">
        <property type="entry name" value="RPP4C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23247">
        <property type="entry name" value="LRR_RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="954"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="6">
        <location>
            <begin position="158"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein At4g27190-like leucine-rich repeats" evidence="7">
        <location>
            <begin position="773"/>
            <end position="868"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9682522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="978" mass="111075" checksum="91DD385B1CDCD478" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DHW6</accession>
    <name>A0AA39DHW6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">60S acidic ribosomal protein P0</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_016840</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">Ribosomal protein P0 is the functional equivalent of E.coli protein L10.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">P0 forms a pentameric complex by interaction with dimers of P1 and P2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the universal ribosomal protein uL10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DHW6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05795">
        <property type="entry name" value="Ribosomal_P0_L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.105.20:FF:000001">
        <property type="entry name" value="60S acidic ribosomal protein P0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.105.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050323">
        <property type="entry name" value="Ribosomal_protein_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001790">
        <property type="entry name" value="Ribosomal_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040637">
        <property type="entry name" value="Ribosomal_uL10-like_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043164">
        <property type="entry name" value="Ribosomal_uL10-like_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043141">
        <property type="entry name" value="Ribosomal_uL10-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030670">
        <property type="entry name" value="uL10_eukaryotes"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45699">
        <property type="entry name" value="60S ACIDIC RIBOSOMAL PROTEIN P0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45699:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00428">
        <property type="entry name" value="Ribosomal_60s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00466">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17777">
        <property type="entry name" value="RL10P_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039087">
        <property type="entry name" value="L10E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160369">
        <property type="entry name" value="Ribosomal protein L10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL10-like insertion" evidence="8">
        <location>
            <begin position="114"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="288"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR039087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9684564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="320" mass="34231" checksum="7487EADD8FBE5948" modified="2024-01-24" version="1">MAVKPSKAEKKIAYDQKLCQLLDEYTQILIAAADNVGSNQLQNIRKGLRGDSVVLMGKNTMMKRSIRLHAEKTGNQAFLNLIPLLVGNVGLIFTRGDLKEVSEEVAKYKVGAPARVGLVAPIDVIVPPGNTGLDPSQTSFFQVLNIPTKINKGTVEIITPVELIKKGDKVGSSEAALLAKLGIRPFSYGLVVLSAYDNGSVFSPEVLDLTEEDLMEKFAAGVSMVTSLSLAISYPTLAAAPHMFINAYKNVLSVAVATEYSFPQADKVKEFLKDPSKFAVAVAPVAAADAGGGAAPPKEEEKKEEPAEESDDDMGFSLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DI49</accession>
    <name>A0AA39DI49_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_016744</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DI49"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005504">
        <property type="term" value="F:fatty acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009627">
        <property type="term" value="P:systemic acquired resistance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00010">
        <property type="entry name" value="AAI_LTSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.110.10">
        <property type="entry name" value="Plant lipid-transfer and hydrophobic proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036312">
        <property type="entry name" value="Bifun_inhib/LTP/seed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016140">
        <property type="entry name" value="Bifunc_inhib/LTP/seed_store"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039265">
        <property type="entry name" value="DIR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33122:SF65">
        <property type="entry name" value="INHIBITOR_LIPID-TRANSFER PROTEIN_SEED STORAGE 2S ALBUMIN SUPERFAMILY PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33122">
        <property type="entry name" value="LIPID BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14368">
        <property type="entry name" value="LTP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47699">
        <property type="entry name" value="Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041371809" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein" evidence="1">
        <location>
            <begin position="28"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical" evidence="2">
        <location>
            <begin position="23"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9684430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="118" mass="12272" checksum="FA647E4ECA63DCCB" modified="2024-01-24" version="1">MVLLKKYVGFLVLLVVLGITGFKGVDAAGECGNASPDTEAWKLAPCAAAAQNEKAAPSKSCCLQVKKIGQNPDCLCAVMLSNTAKSSGIKPEVAVTIPKRCNLADRPVGYKCGAYTLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DIS2</accession>
    <name>A0AA39DIS2_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_015343</name>
        <name evidence="2" type="ORF">PVL29_015345</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DIS2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9686391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9686393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="93" mass="10532" checksum="97EE9FC7E024690D" modified="2024-01-24" version="1">MIELHKQGVVDMSKLIWISFNINTEVCFKDPQTLKLFQHLCIGEEAIMVCVKIGKAVMDQTIECFFIFHKADNGGTVQHFHTHSLFCSCAQTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DK55</accession>
    <name>A0AA39DK55_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_015971</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DK55"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9687296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="116" mass="13233" checksum="AAD8C0FD96C83961" modified="2024-01-24" version="1">MHPLINFPTHRTRLHNHPKPITSAHRRQIKRINVSRLWEPEPTERLELEGDKDVEDEGLSDGEKSNDTRKGNIFGVETGSWVLPFSSSFDQVGRWCEGEKAFPLKKGEGALKGEDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DK61</accession>
    <name>A0AA39DK61_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Brix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_017199</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DK61"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034457">
        <property type="term" value="C:Mpp10 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042134">
        <property type="term" value="F:rRNA primary transcript binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10480:FF:000001">
        <property type="entry name" value="IMP4, U3 small nucleolar ribonucleoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10480">
        <property type="entry name" value="Probable brix-domain ribosomal biogenesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007109">
        <property type="entry name" value="Brix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044281">
        <property type="entry name" value="IMP4/RPF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22734">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RIBONUCLEOPROTEIN PROTEIN IMP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22734:SF2">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RIBONUCLEOPROTEIN PROTEIN IMP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04427">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00879">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50833">
        <property type="entry name" value="BRIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Brix" evidence="1">
        <location>
            <begin position="1"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9685072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="166" mass="19070" checksum="2CABBFD9956A2B9E" modified="2024-01-24" version="1">MKRVVISEIIETCRAHDFTDVVLVHEHRGVPDGIIISHLPFGPTVYFGLLNVVTRHDIKDEKAIGKMSEAYPHLTLNNFSTKLGERTANILKHLFPVPKPDTKRIITFANQSDYISFRHHVYEKRGGPKSIELKEVGPRFELRLYQVTGCLVHFSLIKKKKKKEDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DKQ2</accession>
    <name>A0AA39DKQ2_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_015821</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DKQ2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044659">
        <property type="entry name" value="PELPK1_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33088">
        <property type="entry name" value="MUCIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33088:SF28">
        <property type="entry name" value="PROTEIN PELPK1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041403281" evidence="2">
        <location>
            <begin position="29"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="111"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9687115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="133" mass="14535" checksum="B4EF957E720F0E9B" modified="2024-01-24" version="1">MARHHEPSILLPLLLITLSLMSGKEVLASRHLLETTLPTVPELPKVELPPLPTLPTLPKFELPPLPHVPTLQEPQLPTLPKPELPTVPHVPALEKPELPHLPDLPKPTLPTIPTLPKDIPFPSLSPPHSTTSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DLW7</accession>
    <name>A0AA39DLW7_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_015593</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DLW7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008507">
        <property type="entry name" value="DUF789"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32010:SF23">
        <property type="entry name" value="IG-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32010">
        <property type="entry name" value="PHOTOSYSTEM II STABILITY/ASSEMBLY FACTOR HCF136, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05623">
        <property type="entry name" value="DUF789"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9686812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="538" mass="59981" checksum="62512E2B7B8A4F85" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DMP1</accession>
    <name>A0AA39DMP1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Receptor-like serine/threonine-protein kinase</fullName>
            <ecNumber evidence="16">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">PVL29_012740</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="24">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="16"/>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DMP1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048544">
        <property type="term" value="P:recognition of pollen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00028">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01098">
        <property type="entry name" value="PAN_AP_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.90.10.10:FF:000001">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000467">
        <property type="entry name" value="Liguleless narrow1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001037">
        <property type="entry name" value="Putative G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.10">
        <property type="entry name" value="Bulb-type lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021820">
        <property type="entry name" value="S-locus_recpt_kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000858">
        <property type="entry name" value="S_locus_glycoprot_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024171">
        <property type="entry name" value="SRK-like_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002">
        <property type="entry name" value="RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD1-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002:SF1055">
        <property type="entry name" value="RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11883">
        <property type="entry name" value="DUF3403"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08276">
        <property type="entry name" value="PAN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00954">
        <property type="entry name" value="S_locus_glycop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000641">
        <property type="entry name" value="SRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00473">
        <property type="entry name" value="PAN_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="17" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 16" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 19" id="KW-0732">Signal</keyword>
    <keyword evidence="4 16" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041288726" description="Receptor-like serine/threonine-protein kinase" evidence="19">
        <location>
            <begin position="27"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="434"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="22">
        <location>
            <begin position="27"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="21">
        <location>
            <begin position="284"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="23">
        <location>
            <begin position="339"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
            <begin position="514"/>
            <end position="801"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR000641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KAJ9690236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="832" mass="93157" checksum="60AB7CF3BD19D29F" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DN30</accession>
    <name>A0AA39DN30_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_013119</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DN30"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000044">
        <property type="entry name" value="Putative LRR receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051824">
        <property type="entry name" value="LRR_Rcpt-Like_S/T_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48006">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN DDB_G0281931-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48006:SF66">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="1"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="237"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="240"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9690803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="257" mass="28415" checksum="93E7849BB9C40A3D" modified="2024-01-24" version="1">MENNSLARALFERSVLKLDWATRYKICVGIARGLTFLHEESTIMIVHRDIKATNVLLDGNLNAKISDFGLAKLNEGENTHISTRIAGTIGYMAPEYALWGYLTDKADVYSFGVVTLEIVSGKNNSNYRPDDTCTCLLDWAFVLQQKGSLMELVDPNLGTEFNEKEAERMIKVALLCTNASSKLRPTMSAALRMLEGQDIIPEVISDPSIYGKDMRISPLRDHYQHMEMQSSIGSLAPNFSSDGAQVGSSSSSAQDLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DQE4</accession>
    <name>A0AA39DQE4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Large ribosomal subunit protein uL30-like ferredoxin-like fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_012117</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL30 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9693233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DQE4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01657">
        <property type="entry name" value="Ribosomal_L7_archeal_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1390.20:FF:000024">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.20">
        <property type="entry name" value="Ribosomal protein L30, ferredoxin-like fold domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036919">
        <property type="entry name" value="Ribo_uL30_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039699">
        <property type="entry name" value="Ribosomal_uL30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035808">
        <property type="entry name" value="Ribosomal_uL30_euk_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016082">
        <property type="entry name" value="Ribosomal_uL30_ferredoxin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11524">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11524:SF16">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00327">
        <property type="entry name" value="Ribosomal_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55129">
        <property type="entry name" value="Ribosomal protein L30p/L7e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="20"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL30-like ferredoxin-like fold" evidence="5">
        <location>
            <begin position="177"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9693233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="274" mass="31401" checksum="7AE651CA643A4A8E" modified="2024-01-24" version="1">MKNEDGDDCDDWWWLVHKNIGASFLCIAVFAFLVRGAVSFHPYQVLAIIRSIRTMRLKGHWMEITLNMPAKEWCLVDPPNSNNINLSVGNENIPIKISTQRHNGNRAPQVQFSHDANGLAASILTHFPTKPTILHLGNTLHDHLGMQFFTTQKELIQLKREAKLTGGFYVDPEAKLVFIIRIRGINAMHPKMRKILQLLHLRQALGKFGIICMEDLIHEIMTVGPHFKEANNFLWPFKLKAPLGGLKKRRNHYVEGGDAGNRENYINELIRRMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DUA6</accession>
    <name>A0AA39DUA6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SOSS complex subunit B homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_008530</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DUA6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070876">
        <property type="term" value="C:SOSS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044818">
        <property type="term" value="P:mitotic G2/M transition checkpoint"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010212">
        <property type="term" value="P:response to ionizing radiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000072">
        <property type="entry name" value="SOSS complex subunit B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051231">
        <property type="entry name" value="SOSS-B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13356">
        <property type="entry name" value="OB FOLD NUCLEIC ACID BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13356:SF0">
        <property type="entry name" value="SOSS COMPLEX SUBUNIT B HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9696354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="137" mass="15257" checksum="A78ACF469B179B30" modified="2024-01-24" version="1">MLSLKDMVPAAQNNINTQFIILDKGKTTLEGKYKTCLALVADETAAVHFQLWGDECDAFEGGDIIRLVDGIFSYNRNNLVLRAGKKGKAEKVGEFCMAFVETPNMSEIRWIPDPSNPKKYVQDAVISPHSRIFPPVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DVA7</accession>
    <name>A0AA39DVA7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_010738</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PPR family. PCMP-H subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DVA7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000366">
        <property type="entry name" value="Pentatricopeptide (PPR) repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000125">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001176">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At2g35030, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000031">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046849">
        <property type="entry name" value="E2_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF373">
        <property type="entry name" value="TETRATRICOPEPTIDE-LIKE HELICAL DOMAIN SUPERFAMILY, DYW DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20430">
        <property type="entry name" value="Eplus_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12854">
        <property type="entry name" value="PPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="6">
        <location>
            <begin position="25"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="6">
        <location>
            <begin position="88"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="6">
        <location>
            <begin position="150"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="6">
        <location>
            <begin position="212"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="6">
        <location>
            <begin position="347"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="6">
        <location>
            <begin position="382"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="6">
        <location>
            <begin position="484"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9695422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="578" mass="65404" checksum="17A426609353A1DA" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DYX1</accession>
    <name>A0AA39DYX1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_006461</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DYX1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.690">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041309814" evidence="1">
        <location>
            <begin position="21"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9701124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="142" mass="15383" checksum="AFC51D0F7392F561" modified="2024-01-24" version="1">MPVGLLSLFLSVVFTQFLVCYKLPQMGNRRSLILGSGDIDTSTVVKADSDGCLGGASGASLVVNSPWKNPSGEWHVGYKVVYELCTDTLTSRLKSYDDKGPIIELCFGMENYGGSLLIHSLEDDPGCGEVADFVTVTNYRYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DZU2</accession>
    <name>A0AA39DZU2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein BIG GRAIN 1-like B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_004027</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in auxin transport. Regulator of the auxin signaling pathway.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the BIG GRAIN 1 (BG1) plant protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DZU2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039621">
        <property type="entry name" value="BG1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33541:SF12">
        <property type="entry name" value="PROTEIN BIG GRAIN 1-LIKE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33541">
        <property type="entry name" value="PROTEIN BIG GRAIN 1-LIKE A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0927">Auxin signaling pathway</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="53"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="116"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="116"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9702084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="425" mass="48329" checksum="4FB4DCD9B3ADAB38" modified="2024-01-24" version="1">MNKWEKSLREDKSGRERKNPSFSSSLLDEIYRSITDGDEDHKELHFYRETMAKKHSLRGSRGVREEEMARARGAGVTDKWMQKKCSEKVVSQHQRRSLPEIDRKWQYYQDPLFFSSSSSSSDSSYGGFSSSETESMKSFFAPPPRPRPIRTARLERSEQALSYGQSEFHMFDDYHHHSAITREPSKSEEEIIKSKSRALKIYGNLKKVKQPISPGGRLATFLNSLFTAGNTKKSTKTTSSAGGFEDCSSERKLNSQPSTCSSASSFARSCLNKNSPSVKEKSRNDAKRTVRFFPVSIIVDEDCRPCGRKCVYGEEGSTQVSASRKITKRNEDDLKFPDQTRRVEAAARELHRSFHQKKNDNIFNNFHVNYDEADEDDDAASCSSSDLFELDHLALIGNGRYQEELPVYETTHIDTNRAIANGLLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E118</accession>
    <name>A0AA39E118_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">2-oxoglutarate-dependent dioxygenase DAO</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Protein DIOXYGENASE FOR AUXIN OXIDATION</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_005458</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">2-oxoglutarate-dependent dioxygenase essential for auxin catabolism and maintenance of auxin homeostasis in reproductive organs. Catalyzes the irreversible oxidation of indole-3-acetic acid (IAA) to the biologically inactive 2-oxoindole-3-acetic acid (OxIAA).</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9704183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E118"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.330:FF:000017">
        <property type="entry name" value="2-oxoglutarate-dependent dioxygenase DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050231">
        <property type="entry name" value="Iron_ascorbate_oxido_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47990">
        <property type="entry name" value="2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0408">Iron</keyword>
    <keyword evidence="1 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="7">
        <location>
            <begin position="144"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00054658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00074102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00076740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9704183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="298" mass="32776" checksum="AB9350E1A29C953F" modified="2024-01-24" version="1">MAAGDIPSIDMQDFPEQSRRLREACEEWGCFRVVNHSIPLSLLSEMKAVVGSLLDLPLEVKRRNTDVIAGSGYMAPSKVNPLYESLGLYDMASQQAVDAFCSQLDASPHQRETIKIYAKAVQELAMDIGNKLAETMGLGGDLFKEWPCQFRINKYNFTPETVGSPGVQIHTDSSFLTILQDDENVGGLEVMDKSGAFVAVDPMPGTLLVNLGDIATVWSNGRLCNVKHRVQCKEAAVRLSIASFLLGPKEAAVEAPPELVDSEHPRLFVPFTYEDYRKLRLSTKLQAGEALALVRVDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E264</accession>
    <name>A0AA39E264_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">RCK N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_003479</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E264"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000036">
        <property type="entry name" value="Glutathione-regulated potassium-efflux system protein KefB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1530.20:FF:000011">
        <property type="entry name" value="K(+) efflux antiporter 3, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46157">
        <property type="entry name" value="K(+) EFFLUX ANTIPORTER 3, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46157:SF4">
        <property type="entry name" value="K(+) EFFLUX ANTIPORTER 3, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02254">
        <property type="entry name" value="TrkA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0050">Antiport</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0630">Potassium</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="RCK N-terminal" evidence="11">
        <location>
            <begin position="542"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="791"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="794"/>
            <end position="805"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9705458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="818" mass="89144" checksum="BE0BE0A9444260FD" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E461</accession>
    <name>A0AA39E461_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Homeobox protein knotted-1-like 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_000727</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TALE/KNOX homeobox family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E461"/>
    <dbReference type="SMR" id="A0AA39E461"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000076">
        <property type="entry name" value="Homeobox protein knotted-1-like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005539">
        <property type="entry name" value="ELK_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005540">
        <property type="entry name" value="KNOX1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005541">
        <property type="entry name" value="KNOX2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050224">
        <property type="entry name" value="TALE_homeobox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11850">
        <property type="entry name" value="HOMEOBOX PROTEIN TRANSCRIPTION FACTORS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03789">
        <property type="entry name" value="ELK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03790">
        <property type="entry name" value="KNOX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03791">
        <property type="entry name" value="KNOX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01188">
        <property type="entry name" value="ELK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01255">
        <property type="entry name" value="KNOX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01256">
        <property type="entry name" value="KNOX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51213">
        <property type="entry name" value="ELK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 5" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ELK" evidence="9">
        <location>
            <begin position="215"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="8">
        <location>
            <begin position="235"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox; TALE-type" evidence="5">
        <location>
            <begin position="236"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="193"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="194"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9708866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="318" mass="36295" checksum="E6FDFD9CBA68D57C" modified="2024-01-24" version="1">MEEVYGLNSTSDYSDKVLMSPENLILPADYQSFFSSAAAFRDYRIPVFGSNELISVASAISETASITPEIQREEDVSSVIKAKIASHPCYPRLLEAYIDCQKVGAPPEIACLLDEIRRENDVCKRDAVSTCLGADPELDEFMETYCDMLEKYKSDLARPFDEATTFLNKIEMQLGNLCNDASIRSLPDEAVVSSDEDFSGGEEVQEAQPRGEDQELKERLLRRFGGRISSLKLEFSKKKKKGKLPKEARQTLLEWWNLHYKWPYPTEADKIALAETTGLDQKQINNWFINQRKRHWKPSENMQFAVMDNHSGQFFTDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E4C0</accession>
    <name>A0AA39E4C0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Trypsin family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_000161</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9707963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E4C0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057906">
        <property type="entry name" value="Nal1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057904">
        <property type="entry name" value="Nal1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057905">
        <property type="entry name" value="Nal1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31521">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31521:SF2">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25819">
        <property type="entry name" value="Nal1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25608">
        <property type="entry name" value="NAL1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nal1 N-terminal" evidence="2">
        <location>
            <begin position="98"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="Nal1 C-terminal" evidence="3">
        <location>
            <begin position="190"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="547"/>
            <end position="596"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9707963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="596" mass="64949" checksum="6C62E1E3CBA8938C" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E5E9</accession>
    <name>A0AA39E5E9_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_003654</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E5E9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9705682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="141" mass="14208" checksum="E02DE30588C89DA4" modified="2024-01-24" version="1">MVVGGGERNGIEGIVVGIEGIEGMVGKVVGSGGNEVGAVWPWAKKVGVVGKDGNVGCGMVGRDGKKGIVGLGRVGSEGKVGSEEGKGGNVGFGRVGKIGVVGTEGGAMSKRGRAARLTSMLENESRTKRDKMKQLQEAIAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E5U3</accession>
    <name>A0AA39E5U3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">S-adenosylmethionine decarboxylase proenzyme</fullName>
            <ecNumber evidence="12">4.1.1.50</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PVL29_001125</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>S-adenosyl-L-methionine + H(+) = S-adenosyl 3-(methylsulfanyl)propylamine + CO2</text>
            <dbReference type="Rhea" id="RHEA:15981"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57443"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="4.1.1.50"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </cofactor>
        <text evidence="12">Binds 1 pyruvoyl group covalently per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 12">Amine and polyamine biosynthesis; S-adenosylmethioninamine biosynthesis; S-adenosylmethioninamine from S-adenosyl-L-methionine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the eukaryotic AdoMetDC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.50" evidence="12"/>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E5U3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004014">
        <property type="term" value="F:adenosylmethionine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099402">
        <property type="term" value="P:plant organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008295">
        <property type="term" value="P:spermidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006597">
        <property type="term" value="P:spermine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.50:FF:000001">
        <property type="entry name" value="S-adenosylmethionine decarboxylase proenzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.90.10:FF:000002">
        <property type="entry name" value="S-adenosylmethionine decarboxylase proenzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.50">
        <property type="entry name" value="S-adenosylmethionine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.90.10">
        <property type="entry name" value="S-adenosylmethionine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048283">
        <property type="entry name" value="AdoMetDC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001985">
        <property type="entry name" value="S-AdoMet_decarboxylase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016067">
        <property type="entry name" value="S-AdoMet_deCO2ase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018166">
        <property type="entry name" value="S-AdoMet_deCO2ase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00535">
        <property type="entry name" value="SAM_DCase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11570">
        <property type="entry name" value="S-ADENOSYLMETHIONINE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11570:SF15">
        <property type="entry name" value="S-ADENOSYLMETHIONINE DECARBOXYLASE PROENZYME 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01536">
        <property type="entry name" value="SAM_decarbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001355">
        <property type="entry name" value="S-AdenosylMet_decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56276">
        <property type="entry name" value="S-adenosylmethionine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01336">
        <property type="entry name" value="ADOMETDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="4 12" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="8 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="6 12" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="10 12" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="9 12" id="KW-0704">Schiff base</keyword>
    <keyword evidence="5 12" id="KW-0745">Spermidine biosynthesis</keyword>
    <keyword evidence="7 12" id="KW-0865">Zymogen</keyword>
    <feature type="chain" id="PRO_5042322207" description="S-adenosylmethionine decarboxylase beta chain" evidence="17">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042322208" description="S-adenosylmethionine decarboxylase alpha chain" evidence="17">
        <location>
            <begin position="71"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate; via pyruvic acid" evidence="13">
        <location>
            <position position="71"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor; for catalytic activity" evidence="13">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for processing activity" evidence="13">
        <location>
            <position position="234"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for processing activity" evidence="13">
        <location>
            <position position="247"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Cleavage (non-hydrolytic); by autolysis" evidence="16">
        <location>
            <begin position="70"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="modified residue" description="Pyruvic acid (Ser); by autocatalysis" evidence="15">
        <location>
            <position position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR001355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR001355-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR001355-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR001355-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR001355-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR001355-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ9709493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="358" mass="39715" checksum="505D8430D5B2A006" modified="2024-01-24" version="1">MALPVSAIGFEGYEKRLEISFSEPGIFSDPEGRGLRSLSRAQLDEILEPAECTIVGSLSNDIVDSYVLSESSLFVYPYKIIIKTCGTTKLLLSIPPILKLADILSLSVSCVRYTRGSFNFPGAQPYPHRHFSEEVAVLDSYFGKLGSGSKAYVMGSSDKSQKWHVYSVSAEVRSACDPVYTLEMCMTGLDREMASVFYKTHSSSAVKMTDTSGIRKILPDSEICDFEFEPCGYSMNAIEGAAISTIHVTPEDGFSYASFETVGYNPKYVNLSQLIERVLSCFQPNEFSVAVHADISGKLLERNCLLDVKGYCCEERSNKELGMGGSIVYHRFMKTEGLVSPRSILKCCWKEDDEEEKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E6I7</accession>
    <name>A0AA39E6I7_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_001474</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9710018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E6I7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019275">
        <property type="entry name" value="DUF2301"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36716">
        <property type="entry name" value="F3H9.20 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36716:SF2">
        <property type="entry name" value="F3H9.20 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10063">
        <property type="entry name" value="DUF2301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="179"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="208"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="232"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9710018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="290" mass="31373" checksum="A83804205A2BD2EA" modified="2024-01-24" version="1">MALRLLSATCVTVPKLQISKKRDGFTEARVSSSSSISRVSGRIGSIKCQALGESSVDGVVYQGTYGPWTVDSSDVREVLLYRSGLVTAAASFVVAASAAFWPENSLPSNVINENLDLLYTLGAGGLGLSLFLIHIYVTEIKRTLQALWALGVVGSLATYTTLAQPTGENLIQYVIDNPTAVWFIGPLFAALTGLVFKEGLCYGKLEAGILTFIIPTVLLGHLTGIMDNGVKLTLLASWMALFVIFASRKFTQPIKDDIGDKSVFMFNALAEEEKKALIEKLEQEKLKNLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E7J0</accession>
    <name>A0AA39E7J0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="1">PPIase</shortName>
            <ecNumber evidence="1">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_000959</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclophilin-type PPIase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="1"/>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E7J0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00317">
        <property type="entry name" value="cyclophilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044233">
        <property type="entry name" value="CYP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47511">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE CYP23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47511:SF1">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE CYP23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0697">Rotamase</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041488649" description="Peptidyl-prolyl cis-trans isomerase" evidence="1">
        <location>
            <begin position="25"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="2">
        <location>
            <begin position="35"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9709254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="226" mass="25355" checksum="3823C265D3BA82D5" modified="2024-01-24" version="1">MWRKGAWIVGLLCLALAFVVGASSQEPQLGSARVVFQTNYGDIEFGFFPGVAPKTVDHIFKLVRLGCFNTNHFFRVDRGFVAQVADVVGGRLAPMNEEQRDEAVKTVVGEFSDVKHVRGILSMGRYADPDSASSSFSMLLGDAPHLDGQYAIFGRVTKGDETLKKLEQLPTRREGIFVMPTERITILSSYFYDTKMETCEQEKSMLKRRLAASVVEIERQRMKCFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YH36</accession>
    <name>A0AA38YH36_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">PVL29_026702</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="25">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">Involved in resistance response to the pathogenic oomycetes Phytophthora infestans and Phytophthora capsici.</text>
    </comment>
    <comment type="function">
        <text evidence="20">Promotes hydrogen peroxide H(2)O(2) production and cell death.</text>
    </comment>
    <comment type="subunit">
        <text evidence="21">Interacts with ABCG40.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the leguminous lectin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="25">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YH36"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002229">
        <property type="term" value="P:defense response to oomycetes"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009626">
        <property type="term" value="P:plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06899">
        <property type="entry name" value="lectin_legume_LecRK_Arcelin_ConA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000103">
        <property type="entry name" value="L-type lectin-domain containing receptor kinase IX.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000168">
        <property type="entry name" value="L-type lectin-domain containing receptor kinase IX.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000240">
        <property type="entry name" value="Lectin-domain containing receptor kinase A4.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050528">
        <property type="entry name" value="L-type_Lectin-RKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000985">
        <property type="entry name" value="Lectin_LegA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019825">
        <property type="entry name" value="Lectin_legB_Mn/Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001220">
        <property type="entry name" value="Legume_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27007">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00139">
        <property type="entry name" value="Lectin_legB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00308">
        <property type="entry name" value="LECTIN_LEGUME_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00307">
        <property type="entry name" value="LECTIN_LEGUME_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 22" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0430">Lectin</keyword>
    <keyword evidence="16 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 22" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0611">Plant defense</keyword>
    <keyword evidence="17" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 23" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="277"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="24">
        <location>
            <begin position="343"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="373"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00058054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00058818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00063357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="KAJ9670322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="653" mass="72754" checksum="E6AF7C1494085F60" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YHM0</accession>
    <name>A0AA38YHM0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_026875</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YHM0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20653">
        <property type="entry name" value="CYP81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000023">
        <property type="entry name" value="Cytochrome P450 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050651">
        <property type="entry name" value="Plant_Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47947">
        <property type="entry name" value="CYTOCHROME P450 82C3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47947:SF62">
        <property type="entry name" value="CYTOCHROME P450, FAMILY 81, SUBFAMILY D, POLYPEPTIDE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11 12" id="KW-0349">Heme</keyword>
    <keyword evidence="8 11 12" id="KW-0408">Iron</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 12" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="11">
        <location>
            <position position="439"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9670592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="502" mass="57018" checksum="0D397874D272DFA7" modified="2024-01-24" version="1">MEARWLYSSLSFLFFALAVKFLLQRNKGKRLNLPPSPPGFPIIGHLHLLKGPFHRTLHRLSERHGPIVSLRFGSRPVIVVSSPSAVEECFTKNDVIFANRPKFVMGKYIGYDYTVISLAPYGDHWRNLRRLSAVEIFASNRLNLFLGIRRDEIKKLLLKLSRNSVENFAKVELKSMFSELLLNITMRMVAGKRFYGDDMKDVEEAREFREIVKEIFEFGGTSNPGDFFPILQWIDYQGYNKRALRLGKKLDVFLQGLLDECRSNKSDLENRNTMIDHLLSLQESEPEYYTDEIIKGLIGAIQAGGADTTAGTIEWAMSLLLNHPEVLKKARDELDTHIGHDCLIDETDLPNLQYLQSIISESLRLFPMAPVLVPHLSTEDCKLGGFDVPGGTMLLVNAWALHRDPKLWNDPTSFKPERFETGESETYKLLPFGVGRRACPGIGLANRVIGLTLGSLIQCFDWKRVDEKEIDMAEGQGLIMPKVEPLEAMCKTRQVMNNVSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YJ22</accession>
    <name>A0AA38YJ22_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Trichome birefringence-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_025168</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PC-esterase family. TBL subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YJ22"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016413">
        <property type="term" value="F:O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029962">
        <property type="entry name" value="TBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026057">
        <property type="entry name" value="TBL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025846">
        <property type="entry name" value="TBL_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285:SF30">
        <property type="entry name" value="PROTEIN TRICHOME BIREFRINGENCE-LIKE 42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285">
        <property type="entry name" value="PROTEIN TRICHOME BIREFRINGENCE-LIKE 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13839">
        <property type="entry name" value="PC-Esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14416">
        <property type="entry name" value="PMR5N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041356074" description="Trichome birefringence-like N-terminal domain-containing protein" evidence="7">
        <location>
            <begin position="27"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="Trichome birefringence-like N-terminal" evidence="9">
        <location>
            <begin position="45"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="Trichome birefringence-like C-terminal" evidence="8">
        <location>
            <begin position="98"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF14416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9671339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="374" mass="42408" checksum="D464469D8DAA4D6F" modified="2024-01-24" version="1">MALWACFLVQLAGLLYLLSCVPYIVCGELHINHVDHHTQTMQSDDCDWFLGSWVHDDSYPLYDASNCPFIGTGFDCLKNGRADKEYLKYRWKPNQCDLPRFNGQEFLEGLRGKRMLFVGDSLGFNQWKSLTCMLHSAVPSTNYTFKETKGGGTFSFQEYGVSVMIKKNGFLVDVVVDEKIGRILKLDSISTGNQWLGFDVLIFNSYHWWTHKGRYQTWDYFQVGDELIKEMDRVEAYKIAMTTWAKWVDENIDPSKTRVFFQGVSAVHVIGVDWGEPGAKQCIGQTEPVNGSVYPGGSLPAEDVLRSIISRMKKPAFLMDITLLTQLRKDGHPSVYAGQGKALVDCSHWCLAGVPDSWNELLYAALLGKRNQFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YJU6</accession>
    <name>A0AA38YJU6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Bulb-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_025480</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YJU6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00028">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.90.10.10:FF:000005">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.10">
        <property type="entry name" value="Bulb-type lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32444">
        <property type="entry name" value="BULB-TYPE LECTIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32444:SF235">
        <property type="entry name" value="OS01G0783900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Bulb-type lectin" evidence="4">
        <location>
            <begin position="47"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9671788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="188" mass="20362" checksum="056B7356B750AECD" modified="2024-01-24" version="1">MQHITHSLNVISFKGLCWHRRMFSLCICTDSSTSKFGVITAFLWIFLSTIVLGQSVADGDSLTSSGGTFELGFFTPGSSPHRYVGIWFKKIPVRKVVWVANRLNPVVGSSGVLQIGEDGNLVNMDGQNLTWSTGVSSVSNGSIVELLDSGNLVLRGGDSNGSFIWQGFVDLGKLCFGPNWAQKLKFGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YL62</accession>
    <name>A0AA38YL62_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_025879</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YL62"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025753">
        <property type="entry name" value="AAA_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058017">
        <property type="entry name" value="At3g28540-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050747">
        <property type="entry name" value="Mitochondrial_chaperone_BCS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23070">
        <property type="entry name" value="BCS1 AAA-TYPE ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14363">
        <property type="entry name" value="AAA_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25568">
        <property type="entry name" value="AAA_lid_At3g28540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA-type ATPase N-terminal" evidence="7">
        <location>
            <begin position="28"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="ATPase AAA-type core" evidence="6">
        <location>
            <begin position="232"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase At3g28540-like C-terminal" evidence="8">
        <location>
            <begin position="374"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="295"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="443"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="308"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF25568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9672441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="469" mass="53735" checksum="B26A36DFA7588B3A" modified="2024-01-24" version="1">MESGDMFRNVGSMVGSTMFMWAMFQNHFPQRLGDFIGRCYQKLVNFFYPYIEITFDEFTGKWGARSEAYKDIQTYLGYKSTRQASKLKGGLVKNSRSLVLGIDDHEEVVDVFQGVQVWCISGKQNLNRKSISIYPVRGQSYDRRYYTLTFHKRHWDLISGPYLNYVLREGKALKDRNRQKKLYTNKEGEWNWVAFEHPATFKTLALEPEKKAEIMEDLIAFSENEEYYRRIGTGKSTMIAAMANLLNYDVYDLELTGVENNTELKMLLMEISSKSIIVIEDIDCSLDLTGQRKKAATYEDSEEEEDEKEKKGGKEKGTKTSKVTLSGLLNFIDGLWSACGGERVIVFTTNHIEKLDQALIRKGRMDKHIELSYCSFEAFKVLARNYLNVESHHLFPKISELLREVDMTPADVAEHLTTKTLMKDAGIGLEGLISAIQRKKEARQKKKLSAKGAKSSRKNDRPSAGFCNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YM56</accession>
    <name>A0AA38YM56_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">glutathione transferase</fullName>
            <ecNumber evidence="1">2.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_026249</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3 9">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the GST superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="1"/>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YM56"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03185">
        <property type="entry name" value="GST_C_Tau"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03058">
        <property type="entry name" value="GST_N_Tau"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000018">
        <property type="entry name" value="Glutathione S-transferase U20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000014">
        <property type="entry name" value="Tau class glutathione S-transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045074">
        <property type="entry name" value="GST_C_Tau"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045073">
        <property type="entry name" value="Omega/Tau-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11260">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE, GST, SUPERFAMILY, GST DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11260:SF764">
        <property type="entry name" value="GLUTATHIONE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01152">
        <property type="entry name" value="Main.3:_Omega-_and_Tau-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="5">
        <location>
            <begin position="3"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="6">
        <location>
            <begin position="88"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9672914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="223" mass="25945" checksum="A98012E17C5B2B6D" modified="2024-01-24" version="1">MADEIILLDFWASHFGMRVRIALAEKGLKYEYREENLWNKSPLLLEMNPVHKKVPVLIHNGKPICESLMIVQYIDEVWKDKSPLLPSDPYQRAQARFWADYVDKKVPQLYHELGKKIWYTKGEEHEAGKKEFIECLKFFEGELGEKSYFGGENFGFEDVALVPLSCWFHTYESFGNFSVEGECPKLMAWAKRCSQKESVSSSLADPHKVHGFVMEMRKKLGID</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YMG4</accession>
    <name>A0AA38YMG4_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_026406</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9673112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YMG4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9673112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="184" mass="21030" checksum="EEE185CD7A232147" modified="2024-01-24" version="1">MRDVARKWVSRDGLVGTMMDSKCAVVMAEAFRRRRLQRQLCLFYFSLNSSINQTLLLAKLSIINRRVSYWSCSDKVGIDELVSSFTKLPFATILNAYKSTLIIPKIVESSKGSSGQCFPLITSKSEEYVSIQESTNHIWIPPLKGQVQNKIHNRKVATTEEKIWKQSRKETGKIFLDEASLHRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YQ61</accession>
    <name>A0AA38YQ61_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_023835</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YQ61"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33429:SF2">
        <property type="entry name" value="OS01G0888850 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33429">
        <property type="entry name" value="OS02G0708000 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="25"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9674518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="122" mass="12920" checksum="AC53CA059C895C65" modified="2024-01-24" version="1">MSMPLDQLQHPPPSLSYTSYSRHGSVGPVIAVLAVIAILGTLAAIVGRLCSGRRIMGHGQYDIESWLEEKCSSCIDGRVNPPPLPSADISAGSVPLSIPIHTQEETKPDEPSPQNPRPKGGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YQJ1</accession>
    <name>A0AA38YQJ1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Radical SAM core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_023942</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N(1)-methylguanosine(37) in tRNA(Phe) + pyruvate + S-adenosyl-L-methionine = 4-demethylwyosine(37) in tRNA(Phe) + 5'-deoxyadenosine + L-methionine + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:36347"/>
            <dbReference type="Rhea" id="RHEA-COMP:10164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10165"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64315"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="EC" id="4.1.3.44"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YQJ1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102521">
        <property type="term" value="F:tRNA-4-demethylwyosine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031591">
        <property type="term" value="P:wybutosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013917">
        <property type="entry name" value="tRNA_wybutosine-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034556">
        <property type="entry name" value="tRNA_wybutosine-synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13930">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT TRNA 4-DEMETHYLWYOSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13930:SF0">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT TRNA 4-DEMETHYLWYOSINE SYNTHASE TYW1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08608">
        <property type="entry name" value="Wyosine_form"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Radical SAM core" evidence="11">
        <location>
            <begin position="1"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="299"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="308"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9674713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="321" mass="36794" checksum="BC45A335A64400F0" modified="2024-01-24" version="1">METTPSLACANKCVFCWRHHTNPVGKSWRWKMDDPLEIVNSAIDLHLKMIRQMKGVPGVKAERLSEGLSPRHCALSLVGEPIMYPEINSLIEELHRRRISTFLVTNAQFPEKIKQLKPVTQLYVSVDAATKDSLKAIDRPLFGDFWERFVDSLKALREKQQRTVYRLTLVKGWNTEDIDAYFNLFSIGKPDFVEIKGVTYCGSSATSKLTMENVPWHADVKAFSEALALKSKGEYEVACEHVHSCCVLLAKTEKFKVNNQWFTWIDYEKFHDLVASGRPFNSKDYMAATPSWAVYGAEEGGFDPGQSRYRKERHHRPKTTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YRD1</accession>
    <name>A0AA38YRD1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_024243</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YRD1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF78">
        <property type="entry name" value="OS12G0587133 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9675215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="442" mass="50226" checksum="E34CC489100EE800" modified="2024-01-24" version="1">MDIEKAFDHVNWDFLMEVMSKMGFGHRWISWMKWCCSTATFSILINGSPSGFFHSSRGLRQGDPLSPYLFLFAMEALSQLLSSARNGGFISSFKVGGRGREGLTVSHPLFADDTLIFCDANADQLQYLSWTFMWFEAISGLKVNLSKTEAIPVGEGIPMETLASILGCKIGSLPTTYLGLPLGAPYKSTRVWDAVEERFRKRLSLWKRQYLSKGGRLTLLQSTLSSLPTYFLSLFVIPKRVRARLEKIQRDFLWGGGALENKPHLVSWKTICTTKNDGGLGIRKLATFNKALLGKWLWRFANENDSLWKQIISSKYDLQDGGWCSKVVRDRYGVGVWKAIRNGWEKFQSHSCFIVGDGTRVKFWKDLWCKNHSLEEAFPTLFNLSINKESWVAEAWEEDGVGGSWGPRFNRHLNDWEVGEVESLLGKLLPMTIRRGVDDSLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YS94</accession>
    <name>A0AA38YS94_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">Laccase</fullName>
            <ecNumber evidence="4 13">1.10.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Benzenediol:oxygen oxidoreductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Diphenol oxidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Urishiol oxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_024593</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Lignin degradation and detoxification of lignin-derived products.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>4 hydroquinone + O2 = 4 benzosemiquinone + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:11276"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17594"/>
            <dbReference type="ChEBI" id="CHEBI:17977"/>
            <dbReference type="EC" id="1.10.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="13">Binds 4 Cu cations per monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Secreted</location>
            <location evidence="2 13">Extracellular space</location>
            <location evidence="2 13">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the multicopper oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.10.3.2" evidence="4 13"/>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YS94"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052716">
        <property type="term" value="F:hydroquinone:oxygen oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046274">
        <property type="term" value="P:lignin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13849">
        <property type="entry name" value="CuRO_1_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13875">
        <property type="entry name" value="CuRO_2_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13897">
        <property type="entry name" value="CuRO_3_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033138">
        <property type="entry name" value="Cu_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002355">
        <property type="entry name" value="Cu_oxidase_Cu_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034288">
        <property type="entry name" value="CuRO_1_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034285">
        <property type="entry name" value="CuRO_2_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034289">
        <property type="entry name" value="CuRO_3_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017761">
        <property type="entry name" value="Laccase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03389">
        <property type="entry name" value="laccase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF487">
        <property type="entry name" value="LACCASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00079">
        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00080">
        <property type="entry name" value="MULTICOPPER_OXIDASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0052">Apoplast</keyword>
    <keyword evidence="10 13" id="KW-0186">Copper</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 13" id="KW-0439">Lignin degradation</keyword>
    <keyword evidence="7 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 13" id="KW-0964">Secreted</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041488380" description="Laccase" evidence="13">
        <location>
            <begin position="25"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="16">
        <location>
            <begin position="35"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="14">
        <location>
            <begin position="161"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="15">
        <location>
            <begin position="419"/>
            <end position="552"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9675741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="569" mass="63567" checksum="53128F00D5FEC190" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YSI2</accession>
    <name>A0AA38YSI2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase</fullName>
            <ecNumber evidence="3">3.2.2.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_024643</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>NAD(+) + H2O = ADP-D-ribose + nicotinamide + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16301"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57967"/>
            <dbReference type="EC" id="3.2.2.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:16302"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the disease resistance TIR-NB-LRR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.6" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061809">
        <property type="term" value="F:NAD+ nucleosidase activity, cyclic ADP-ribose generating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050832">
        <property type="term" value="P:defense response to fungus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043068">
        <property type="term" value="P:positive regulation of programmed cell death"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10140:FF:000007">
        <property type="entry name" value="Disease resistance protein (TIR-NBS-LRR class)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045344">
        <property type="entry name" value="C-JID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011713">
        <property type="entry name" value="Leu-rich_rpt_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058192">
        <property type="entry name" value="WHD_ROQ1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017:SF570">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN (TIR-NBS CLASS)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20160">
        <property type="entry name" value="C-JID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07725">
        <property type="entry name" value="LRR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23282">
        <property type="entry name" value="WHD_ROQ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0611">Plant defense</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="TIR" evidence="14">
        <location>
            <begin position="23"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="TIR" evidence="14">
        <location>
            <begin position="216"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1433"/>
            <end position="1469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00061488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9675800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1502" mass="170798" checksum="1FAA76E24B8F4D4C" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YVQ8</accession>
    <name>A0AA38YVQ8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ATP synthase subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_022524</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and the central stalk which is part of the complex rotary element. The gamma subunit protrudes into the catalytic domain formed of alpha(3)beta(3). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) and CF(0) have multiple subunits.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the ATPase gamma chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YVQ8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12151">
        <property type="entry name" value="F1-ATPase_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.80:FF:000001">
        <property type="entry name" value="ATP synthase gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1380.10:FF:000005">
        <property type="entry name" value="ATP synthase subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1380.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.80">
        <property type="entry name" value="ATP synthase, gamma subunit, helix hairpin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035968">
        <property type="entry name" value="ATP_synth_F1_ATPase_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000131">
        <property type="entry name" value="ATP_synth_F1_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023632">
        <property type="entry name" value="ATP_synth_F1_gsu_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01146">
        <property type="entry name" value="ATPsyn_F1gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11693">
        <property type="entry name" value="ATP SYNTHASE GAMMA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11693:SF22">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT GAMMA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00231">
        <property type="entry name" value="ATP-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039089">
        <property type="entry name" value="ATP_synthase_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00126">
        <property type="entry name" value="ATPASEGAMMA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52943">
        <property type="entry name" value="ATP synthase (F1-ATPase), gamma subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00153">
        <property type="entry name" value="ATPASE_GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 14" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="12 14" id="KW-0139">CF(1)</keyword>
    <keyword evidence="6 14" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="9 14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="5 14" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9677583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="322" mass="35151" checksum="E8075A40E22C9827" modified="2024-01-24" version="1">MAMAAFRREGRRFAPLITPRPIAAVRSSPIPEEQAPLGVRSISTQVVRNRMKSVKNIQKITKAMKMVAASKLRAVQTRAENSRGLWQPFTVLLGDSPSVDVKKNVIVTISSDKGLCGGINSTSVKTSKALHKFTSGPEKGTKYVVLGEKAKAQLMRDSKKDIELCITELQKNPLNYTQVSVLADEILKNVEYDALRIVFNKFRSVVSFVPTTSTVLSPEVVEREAEAGGKLGDLDSYEIEGGETKGEILQNLAEFQFSCVLFNAVLENACSEQGARMSAMDSSSRNAGDMLDRLTLTYNRTRQAAITTELTEIISGASALEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YY32</accession>
    <name>A0AA38YY32_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PGG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_020695</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YY32"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026961">
        <property type="entry name" value="PGG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24128:SF61">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN BDA1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24128">
        <property type="entry name" value="HOMEOBOX PROTEIN WARIAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13962">
        <property type="entry name" value="PGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="277"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="332"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="368"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="391"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="69"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="103"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="137"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="PGG" evidence="3">
        <location>
            <begin position="275"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9678598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="430" mass="47478" checksum="2DF23CDC91A0CE4D" modified="2024-01-24" version="1">MDRRLLDAAQAGKVEDLHQLLRENPLVLHTTALASAENPLHISSISGHVDFVKELIRLKPDFIKELNQDGFSPIHMAAANGRQEVVMELLKFDWKLCHLEGRDEKTPLHCAAMKGKVNVVQVILSACKECIEDVTVQKETALHLAVKNSQYEAVRVLVEKVREMKREDVIEFLLGDATIPGSGVTEVNLMNKTGLTALDVLLIFPSEAGDREIKEILHSAGAKRAQDIAFPPASFGTQNHARLNRTTTVETCSMQPNNLVNYFRFHRGRDAPGEVRSALLVIAVLVATATYQVGLSPPGGVWQDNSGTNQSNSTATNKAHFAGQSIFSTLGIISYGIFVLFNSIGFSVSLYIISILTSKFPMHFELQICLLAMFFTYNTAIITISPVNLKIFLIVLTSILPLIVSLVATWVREYVKKITKLAADMVNRIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YYK4</accession>
    <name>A0AA38YYK4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DUF659 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_021103</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YYK4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007021">
        <property type="entry name" value="DUF659"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008906">
        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32166:SF122">
        <property type="entry name" value="OS09G0499600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32166">
        <property type="entry name" value="OSJNBA0013A04.12 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04937">
        <property type="entry name" value="DUF659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF659" evidence="2">
        <location>
            <begin position="42"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="HAT C-terminal dimerisation" evidence="3">
        <location>
            <begin position="394"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="538"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="538"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="554"/>
            <end position="572"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9679071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="783" mass="89817" checksum="12274063EA35DD6D" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YZA0</accession>
    <name>A0AA38YZA0_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_021227</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YZA0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9679228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="68" mass="7189" checksum="8614184F56AA038B" modified="2024-01-24" version="1">MAAAAYDVPLLALKGINANLNFPNSALSFHIPKSTSAGDIRAAVVTSEYRQGSASEVQELGEPRHHPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YZE4</accession>
    <name>A0AA38YZE4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable RNA-binding protein 18</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">RNA-binding motif protein 18</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_021437</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YZE4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12355">
        <property type="entry name" value="RRM_RBM18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039157">
        <property type="entry name" value="RBM18_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21245">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="13"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="100"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="109"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00021141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00030780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9679515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="170" mass="18778" checksum="CBFCC5E97BB00CFF" modified="2024-01-24" version="1">MDPNCLTDKKCECRLFIGNLDKRVSEVALIKMFSPFGKIVGEDFMWHTCGPKRGEPRGFAFIEFSTKEEAELAKEKMHGKLICGRPLTVRLANEKSLMETAEAAPQRVGASNKSSLTGRSSGQMSRSAKIAAIKNKLKEMEEESNGTKRQKVADKVSCSNGLDRSSTKES</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z009</accession>
    <name>A0AA38Z009_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_021639</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z009"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000590">
        <property type="entry name" value="PR5-like receptor kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045874">
        <property type="entry name" value="LRK10/LRL21-25-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025287">
        <property type="entry name" value="WAK_GUB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27009">
        <property type="entry name" value="RUST RESISTANCE KINASE LR10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13947">
        <property type="entry name" value="GUB_WAK_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041221766" description="Protein kinase domain-containing protein" evidence="13">
        <location>
            <begin position="29"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="367"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="395"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9679789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="676" mass="77097" checksum="3C28007963B6C622" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2025-10-08" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z1J0</accession>
    <name>A0AA38Z1J0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">CCR4-NOT transcription complex subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_019864</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030015">
        <property type="term" value="C:CCR4-NOT core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000289">
        <property type="term" value="P:nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20710">
        <property type="entry name" value="NOT1_connector"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000012">
        <property type="entry name" value="Ccr4-Not transcription complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.800:FF:000001">
        <property type="entry name" value="CCR4-NOT transcription complex subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.790:FF:000002">
        <property type="entry name" value="Transcription regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.840:FF:000003">
        <property type="entry name" value="Transcription regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.800">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.840">
        <property type="entry name" value="CCR4-NOT transcription complex subunit 1 TTP binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007196">
        <property type="entry name" value="CCR4-Not_Not1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055454">
        <property type="entry name" value="CNOT1-like_NOT1_connector"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032191">
        <property type="entry name" value="CNOT1_CAF1_bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024557">
        <property type="entry name" value="CNOT1_dom_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032194">
        <property type="entry name" value="CNOT1_HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032193">
        <property type="entry name" value="CNOT1_TTP_bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038535">
        <property type="entry name" value="CNOT1_TTP_bind_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040398">
        <property type="entry name" value="Not1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13162">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13162:SF8">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25097">
        <property type="entry name" value="ARM_Cnot1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16415">
        <property type="entry name" value="CNOT1_CAF1_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16418">
        <property type="entry name" value="CNOT1_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16417">
        <property type="entry name" value="CNOT1_TTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12842">
        <property type="entry name" value="DUF3819"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04054">
        <property type="entry name" value="Not1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1 HEAT repeat" evidence="11">
        <location>
            <begin position="474"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1 TTP binding" evidence="10">
        <location>
            <begin position="641"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1 CAF1-binding" evidence="9">
        <location>
            <begin position="964"/>
            <end position="1184"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1" evidence="8">
        <location>
            <begin position="1310"/>
            <end position="1451"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1-like NOT1 connector" evidence="12">
        <location>
            <begin position="1660"/>
            <end position="1832"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-Not complex component Not1 C-terminal" evidence="7">
        <location>
            <begin position="2037"/>
            <end position="2398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1501"/>
            <end position="1523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1845"/>
            <end position="1880"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1846"/>
            <end position="1872"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF25097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9680673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2422" mass="268926" checksum="3CA7E7AE9252A041" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z1W3</accession>
    <name>A0AA38Z1W3_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_019997</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the synaptotagmin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z1W3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00030">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21677">
        <property type="entry name" value="SMP_SYT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000130">
        <property type="entry name" value="synaptotagmin-4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031468">
        <property type="entry name" value="SMP_LBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045050">
        <property type="entry name" value="Synaptotagmin_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039010">
        <property type="entry name" value="Synaptotagmin_SMP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10774:SF190">
        <property type="entry name" value="C2 CALCIUM_LIPID-BINDING ENDONUCLEASE_EXONUCLEASE_PHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10774">
        <property type="entry name" value="EXTENDED SYNAPTOTAGMIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17047">
        <property type="entry name" value="SMP_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51847">
        <property type="entry name" value="SMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="10" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="domain" description="SMP-LTD" evidence="16">
        <location>
            <begin position="66"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="15">
        <location>
            <begin position="247"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="493"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="391"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="496"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9680853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="524" mass="56438" checksum="9987CF1816D629D5" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z2F3</accession>
    <name>A0AA38Z2F3_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_020095</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ARR-like family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9681020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z2F3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009736">
        <property type="term" value="P:cytokinin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048511">
        <property type="term" value="P:rhythmic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17582">
        <property type="entry name" value="psREC_PRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045279">
        <property type="entry name" value="ARR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010402">
        <property type="entry name" value="CCT_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43874">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43874:SF146">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR-LIKE APRR9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06203">
        <property type="entry name" value="CCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51017">
        <property type="entry name" value="CCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="Response regulatory" evidence="12">
        <location>
            <begin position="52"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="CCT" evidence="13">
        <location>
            <begin position="662"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="318"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="571"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="614"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="689"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="9"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="703"/>
            <end position="714"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9681020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="714" mass="78829" checksum="1C94B0BE139194B6" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z3N9</accession>
    <name>A0AA38Z3N9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_017944</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10 15">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 15">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PP2C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9681811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z3N9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000013">
        <property type="entry name" value="probable protein phosphatase 2C 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="12">
        <location>
            <begin position="28"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9681811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="430" mass="46270" checksum="32E5F78418273025" modified="2024-01-24" version="1">MSETEVSRMKSSLVPLATLIGRELRNDKVEKPYIKYGQAALAKKGEDYFLIKADCQRVPGNPSTSFSVFAIFDGHNGISAAIFAKENLLNNVLSAIPQGIGREGWLQALPRALVAGFVKTDIEFQQKGETSGTTVTFVVIDGWTVTVASVGDSRCILDTQGGVVSLLTVDHRLEENAEERERVTASGGEVGRLNVFGGNEVGPLRCWPGGLCLSRSIGDTDVGEFIVPIPHVKQVKLSNAGGRLIIASDGIWDALSSDMAAKSCRGLPAELAAKLVVKEALRSRGLKDDTTCLVVDMIPSDNPVLPPTPRKKQNMLSSFIFGKRSQNFMSKSTNKLSAVGVVEELFEEGSAMLAERLGKDFPSNTNSGLFRCAVCQVDQDQSPSDGLSVNSGPFFSPASKPWEGPFLCANCRKKKDAMEGKRPSRPTITA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2025-10-08" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z4E6</accession>
    <name>A0AA38Z4E6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_018194</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057135">
        <property type="entry name" value="At4g27190-like_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050905">
        <property type="entry name" value="Plant_NBS-LRR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463:SF198">
        <property type="entry name" value="RPP4C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23247">
        <property type="entry name" value="LRR_RPS2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0611">Plant defense</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="167"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="1325"/>
            <end position="1464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9682200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="2212" mass="251580" checksum="4835F740F391F139" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z5D2</accession>
    <name>A0AA38Z5D2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_018597</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S1C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z5D2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06786">
        <property type="entry name" value="cpPDZ1_ScNma111-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06787">
        <property type="entry name" value="cpPDZ_AthDEGP7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025926">
        <property type="entry name" value="PDZ-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001940">
        <property type="entry name" value="Peptidase_S1C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46366:SF1">
        <property type="entry name" value="PDZ DOMAIN-CONTAINING PROTEIN C1685.05"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46366">
        <property type="entry name" value="PRO-APOPTOTIC SERINE PROTEASE NMA111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12812">
        <property type="entry name" value="PDZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13180">
        <property type="entry name" value="PDZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13365">
        <property type="entry name" value="Trypsin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00834">
        <property type="entry name" value="PROTEASES2C"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="3">
        <location>
            <begin position="269"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="515"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="517"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="529"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9682701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1083" mass="119538" checksum="B38F17C8386C57F7" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z5Z8</accession>
    <name>A0AA38Z5Z8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Chloroplastic group IIB intron splicing facilitator CRS2, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_018734</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PTH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z5Z8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004045">
        <property type="term" value="F:peptidyl-tRNA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1470:FF:000001">
        <property type="entry name" value="Peptidyl-tRNA hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1470">
        <property type="entry name" value="Peptidyl-tRNA hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00083">
        <property type="entry name" value="Pept_tRNA_hydro_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001328">
        <property type="entry name" value="Pept_tRNA_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018171">
        <property type="entry name" value="Pept_tRNA_hydro_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036416">
        <property type="entry name" value="Pept_tRNA_hydro_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00447">
        <property type="entry name" value="pth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17224:SF3">
        <property type="entry name" value="CHLOROPLASTIC GROUP IIB INTRON SPLICING FACILITATOR CRS2-B, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17224">
        <property type="entry name" value="PEPTIDYL-TRNA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01195">
        <property type="entry name" value="Pept_tRNA_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53178">
        <property type="entry name" value="Peptidyl-tRNA hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01195">
        <property type="entry name" value="PEPT_TRNA_HYDROL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01196">
        <property type="entry name" value="PEPT_TRNA_HYDROL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU004320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9682862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="246" mass="27263" checksum="A0B4D60E76E65537" modified="2024-01-24" version="1">MLYAVSGPSTSISYRRSLDYHGFHRRRLAPARLCVSASLPDANGIKVEYTPWLIVGLGNPGNKYHGTRHNVGFEMIDRISQEEGILLNTIQSKALIGIGSIGEVPILLAKPQAYMNFSGESVGPLAAYYQVPLRHILVAYDEMNLPNGVLRLAPRGGHGYHNGLKNVMGHLDGCREFPRLCIGIGNPPGTMDMKAYLLQKFSFEERKQIDAAQEQGIGAVRNLVLNGFNHSTTRFNLGQKYKYHKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z7M1</accession>
    <name>A0AA38Z7M1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Apyrase 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_016424</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GDA1/CD39 NTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9683913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z7M1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017110">
        <property type="term" value="F:nucleoside diphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009134">
        <property type="term" value="P:nucleoside diphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd24043">
        <property type="entry name" value="ASKHA_NBD_AtAPY7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.150">
        <property type="entry name" value="Exopolyphosphatase. Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000407">
        <property type="entry name" value="GDA1_CD39_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11782">
        <property type="entry name" value="ADENOSINE/GUANOSINE DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11782:SF125">
        <property type="entry name" value="APYRASE 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01150">
        <property type="entry name" value="GDA1_CD39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="120"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="606"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="740"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="294"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="325"/>
            <end position="329"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR600407-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR600407-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9683913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="770" mass="84816" checksum="735722A7DE14EEEF" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZBM5</accession>
    <name>A0AA38ZBM5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein DETOXIFICATION</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Multidrug and toxic compound extrusion protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_015075</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZBM5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13132">
        <property type="entry name" value="MATE_eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045069">
        <property type="entry name" value="MATE_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="51"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="97"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="132"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="170"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="201"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="229"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="272"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="353"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="397"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="423"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="451"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9686016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="504" mass="54886" checksum="2301D5AADE0B6184" modified="2024-01-24" version="1">MGSEEYQPLLLGLDSHARIPDLSSFAVEEFLTHKPVAVRWWPRLFGWESRLLWLLSGSSIVASIFNYMLSFVTLMFTGHLGALELAGASVASVGIQGLAYGIMLGMASAVQTVCGQAYGAKKYKAMGIICQRAIILHLGAAVLLTFLYWFSGAFLRVIGQSDSISAQGQIFARGLILQLYAFAISCPMQRFLQAQNIVNPLAYMAVGVFLLHVLLTWLVVYVLDYGLLGAALTLSFSWWILVVVIALYILLSPSCKETWTGFSSKAFKGMWPYFKLTVASAIMLCLEIWYNQGLVLISGLLSNPTISLDSISICMNYLNWDMQFMLGLSAATSVRVSNELGASHPKVAKLSVLVVNTNSIIISIFFSAIIMIFKVGLSKLFTSDAEVIEAVSNLTPLLAISVFLNGIQPILSGVAIGSGWQAIVAYVNLATYYLIGLPIGCVLGFKTSLGVAGIWWGMIIGVLLQTVTLIILTARTDWNAEVLKAAERLKNSANVENLNLLEDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZBV3</accession>
    <name>A0AA38ZBV3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">phosphoenolpyruvate carboxylase</fullName>
            <ecNumber evidence="5">4.1.1.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_015166</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PEPCase type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.31" evidence="5"/>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZBV3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008964">
        <property type="term" value="F:phosphoenolpyruvate carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015977">
        <property type="term" value="P:carbon fixation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048366">
        <property type="term" value="P:leaf development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1440.90:FF:000001">
        <property type="entry name" value="Phosphoenolpyruvate carboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.90">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00595">
        <property type="entry name" value="PEPcase_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021135">
        <property type="entry name" value="PEP_COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022805">
        <property type="entry name" value="PEP_COase_bac/pln-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018129">
        <property type="entry name" value="PEP_COase_Lys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033129">
        <property type="entry name" value="PEPCASE_His_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000584">
        <property type="entry name" value="PRK00009.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30523">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30523:SF47">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE CARBOXYLASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00311">
        <property type="entry name" value="PEPcase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00150">
        <property type="entry name" value="PEPCARBXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00781">
        <property type="entry name" value="PEPCASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00393">
        <property type="entry name" value="PEPCASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0120">Carbon dioxide fixation</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="10">
        <location>
            <position position="172"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="600"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9686145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="965" mass="110452" checksum="C197FA1BF37DC0DF" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZCM3</accession>
    <name>A0AA38ZCM3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_015367</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZCM3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032440">
        <property type="term" value="F:2-alkenal reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000121">
        <property type="entry name" value="Prostaglandin reductase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041694">
        <property type="entry name" value="ADH_N_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045010">
        <property type="entry name" value="MDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43205:SF80">
        <property type="entry name" value="2-ALKENAL REDUCTASE (NADP(+)-DEPENDENT)-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43205">
        <property type="entry name" value="PROSTAGLANDIN REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16884">
        <property type="entry name" value="ADH_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="2">
        <location>
            <begin position="77"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9686434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="361" mass="39522" checksum="F352A1D6611B6E77" modified="2024-01-24" version="1">MAGLEGVDNGGDENLTVVETREWIMLAYAAKGIPTSDHLKLRTTKLSLDADSIPKDHVAVELLWVSVDPYLRGRMSGQDEGLYFSQFALDEVITTCGIGRVIRSKSSKFSKGDLVSSSFLPFSEYSIIGATFLQVVDQTAGIEISSYLSTLGLPGFAAWVGIEVIGNPKSGSNVFISAAAGGVGMFAGQLAKFKGCRVVGSTGTDEKVKLLKEEFGYDEAFNYNKETDFDAALSKYFPNGIDLYLDNVGGKMLEAVLNHVNVRARIAVSGMISQYNQVWTERVGVRNLLNIVGKEVRIEGYLMGSHMDRFDDFTKEMVTYTKEGKLRSKHKIYYGIESFIESLGSIFSSSNIGKVVIQVKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZDC5</accession>
    <name>A0AA38ZDC5_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_015590</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZDC5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000320">
        <property type="entry name" value="Protein phosphatase 1 regulatory subunit pprA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15454">
        <property type="entry name" value="NISCHARIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15454:SF7">
        <property type="entry name" value="OS07G0106100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52075">
        <property type="entry name" value="Outer arm dynein light chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="121"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="579"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="123"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="579"/>
            <end position="589"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9686809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="672" mass="74374" checksum="99C5FA4D6A3983C0" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZFK0</accession>
    <name>A0AA38ZFK0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transcription termination factor MTERF4, chloroplastic</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Mitochondrial transcription termination factor 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_014071</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mTERF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZFK0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032502">
        <property type="term" value="P:developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.70.10:FF:000004">
        <property type="entry name" value="Transcription termination factor mterf4, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.70.10:FF:000005">
        <property type="entry name" value="Transcription termination factor MTERF4, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.70.10">
        <property type="entry name" value="Transcription termination factor 3, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003690">
        <property type="entry name" value="MTERF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038538">
        <property type="entry name" value="MTERF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13068">
        <property type="entry name" value="CGI-12 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13068:SF24">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02536">
        <property type="entry name" value="mTERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00733">
        <property type="entry name" value="Mterf"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="5" id="KW-0934">Plastid</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0806">Transcription termination</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00071985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00077581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9688155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="481" mass="54924" checksum="9D9AA9A59DBD4234" modified="2024-01-24" version="1">MISLLRRTKLLTLTLTSNPRTLRPFLRFLSSSSKFPEYEMPSVTWGVVQGRKERLVSRVIISDYLKTLGIIPDELEQVELPSTVEVMRERVEFLQKLGLTIDHLNEYPLMLGCSVRKNMIPVLGYLEKIGIPRSKLGEFVVNYPQVLHASVVVELAPVVKFLRGLDVDKQDIGYVLMKYPELLGFKLEGTMSTSVAYLVSIGVSPRDIGPMVTQYPYFLGMRVGTVIKPISDYLVSLGLPKKVLARMFEKRAYVLGYDLEECIKPNVDCLVSFGIRREALASVIAQFPQILGLPLKAKLSSQQYFFNLKLKIDPDGFARVIERMPQIVSLNQNVIMKPVEFLLGRGIPSVDVAKMVVKCPQLVALRVELMKNSFYFFKSEMGRQVKELVEFPEYFTYSLESRIKPRYQRLQSKGVRSSLDWFLNCSDQRFEERLQADYIEMETVGPSFCMGGKLELPGNEVVSDEEDESDDEELYRRTVSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZG11</accession>
    <name>A0AA38ZG11_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tubulin--tyrosine ligase-like protein 12 SET-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_013930</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9687942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZG11"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057954">
        <property type="entry name" value="SET_TTL12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027749">
        <property type="entry name" value="TTLL12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46088">
        <property type="entry name" value="TUBULIN--TYROSINE LIGASE-LIKE PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46088:SF1">
        <property type="entry name" value="TUBULIN--TYROSINE LIGASE-LIKE PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25556">
        <property type="entry name" value="SET_TTL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin--tyrosine ligase-like protein 12 SET-like" evidence="1">
        <location>
            <begin position="70"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin--tyrosine ligase-like protein 12 SET-like" evidence="1">
        <location>
            <begin position="388"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9687942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="870" mass="99588" checksum="DE47D521A1234246" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZGA1</accession>
    <name>A0AA38ZGA1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">20 kDa chaperonin, chloroplastic</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Chaperonin 10</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Protein Cpn21</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_013892</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the GroES chaperonin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9687883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZGA1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00320">
        <property type="entry name" value="cpn10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.33.40:FF:000001">
        <property type="entry name" value="10 kDa chaperonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.33.40:FF:000005">
        <property type="entry name" value="20 kDa chaperonin, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.33.40">
        <property type="entry name" value="GroES chaperonin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00580">
        <property type="entry name" value="CH10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020818">
        <property type="entry name" value="Chaperonin_GroES"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037124">
        <property type="entry name" value="Chaperonin_GroES_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018369">
        <property type="entry name" value="Chaprnonin_Cpn10_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017416">
        <property type="entry name" value="Cpn20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001531">
        <property type="entry name" value="PRK00364.2-2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10772">
        <property type="entry name" value="10 KDA HEAT SHOCK PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10772:SF63">
        <property type="entry name" value="20 KDA CHAPERONIN, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00166">
        <property type="entry name" value="Cpn10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038157">
        <property type="entry name" value="Chaperonin_21_chloroplast"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00297">
        <property type="entry name" value="CHAPERONIN10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00883">
        <property type="entry name" value="Cpn10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00681">
        <property type="entry name" value="CHAPERONINS_CPN10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00031971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00073031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00079398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9687883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="251" mass="26380" checksum="7081DC16EDDCC96B" modified="2024-01-24" version="1">MATAPTISTRSLPSFEGLRPSTVRISSMGLPLKAGGLTQRSFRSLVVRAATVVAPKYTSLKPLGDRVLVKIKTAEEKTVGGILLPTTAQTKPQGGEVVAVGEGKTIGKNKLDICVKTGAQVVYSKYAGTEVEFNGSNHLILKEDDIVGILETDDVKDLKPLNDRVLIKVAEAEEKTAGGLLLTEASKEKPSIGTVVAVGPGPLDEEGKRKPLSVSPGNTVLYSKYAGNDFKGSDGSDYIALKASDIMAVLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZH88</accession>
    <name>A0AA38ZH88_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_014596</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9689025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZH88"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9689025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="114" mass="12921" checksum="CB5EAE569ADEEC7D" modified="2024-01-24" version="1">MYRKFETSSSSNQIPQRALPAGAIHWEPPPEPAHGWKNMRPNVLKSLHIYEYNAGISGSEQKISSLSESTDKKNTSAEKQSRKENWVRFPGQLWSSVSTDFHSTNQTLFCVVVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZIX2</accession>
    <name>A0AA38ZIX2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cystatin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_012535</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9689916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZIX2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004869">
        <property type="term" value="F:cysteine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00042">
        <property type="entry name" value="CY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000010">
        <property type="entry name" value="Cystatin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046350">
        <property type="entry name" value="Cystatin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47364">
        <property type="entry name" value="CYSTEINE PROTEINASE INHIBITOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47364:SF2">
        <property type="entry name" value="CYSTEINE PROTEINASE INHIBITOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16845">
        <property type="entry name" value="SQAPI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00043">
        <property type="entry name" value="CY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54403">
        <property type="entry name" value="Cystatin/monellin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0789">Thiol protease inhibitor</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041274974" description="Cystatin domain-containing protein" evidence="3">
        <location>
            <begin position="24"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="Cystatin" evidence="4">
        <location>
            <begin position="24"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9689916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="112" mass="12078" checksum="A27A13DF04C7A257" modified="2024-01-24" version="1">MARGQYRLSLLLASLLLVAVASGALLGGWSPIKDVKNPHVREIGKFAVGAHNKLAVTKLKFQSVIKGQTQVVAGTNYRLTVMAKNGAASKKYKAVVWEKLDGTKELTSFQPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZJ37</accession>
    <name>A0AA38ZJ37_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Regulatory particle non-ATPase 13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_014715</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9689189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZJ37"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061133">
        <property type="term" value="F:endopeptidase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13314">
        <property type="entry name" value="PH_Rpn13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.2020.20:FF:000002">
        <property type="entry name" value="26S proteasome regulatory subunit RPN13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.70:FF:000001">
        <property type="entry name" value="Proteasomal ubiquitin receptor ADRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.2020.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.70">
        <property type="entry name" value="Proteasomal ubiquitin receptor Rpn13/ADRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044867">
        <property type="entry name" value="DEUBAD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006773">
        <property type="entry name" value="Rpn13/ADRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044868">
        <property type="entry name" value="Rpn13/ADRM1_Pru"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038633">
        <property type="entry name" value="Rpn13/ADRM1_Pru_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032368">
        <property type="entry name" value="RPN13_DEUBAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038108">
        <property type="entry name" value="RPN13_DEUBAD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12225">
        <property type="entry name" value="ADHESION REGULATING MOLECULE 1 110 KDA CELL MEMBRANE GLYCOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12225:SF0">
        <property type="entry name" value="PROTEASOMAL UBIQUITIN RECEPTOR ADRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04683">
        <property type="entry name" value="Rpn13_ADRM1_Pru"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16550">
        <property type="entry name" value="RPN13_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51916">
        <property type="entry name" value="DEUBAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51917">
        <property type="entry name" value="PRU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0647">Proteasome</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pru" evidence="8">
        <location>
            <begin position="11"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="DEUBAD" evidence="7">
        <location>
            <begin position="194"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="293"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9689189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="320" mass="35730" checksum="50C1707472F9EC05" modified="2024-01-24" version="1">MGSSSADTFPVMQEIMLEFRAGKMVFEGTRVTPDTRKGLVRIARGEEGLVHFQWLDRTNNVLEDDQIVFPDEAVFEKVNQASGRVYILKFNTDNRKFFFWMQEPKAEGDSQLCNSVNYHINRPLEFLGEEEQDVSGPVQISEDMVEDDISSRAGNLVGPSLGAEVTSDVTSLSGPVKLADLQRILSNIEPADGAGDPDEGLGLADILKPDLILPLIETLPMEQRLASYLPEGQWNPEDLLELLQSPPFRQQVDSFTYVLRTGQIDLSQFGIDPSKYKFTVLSFLEALEDTVCKASQSGQDDKDSRSQTSRNDNEPMDEAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZJE2</accession>
    <name>A0AA38ZJE2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">U3 small nucleolar RNA-associated protein 6 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_012420</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UTP6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9689712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZJE2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034388">
        <property type="term" value="C:Pwp2p-containing subcomplex of 90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000563">
        <property type="entry name" value="U3 small nucleolar RNA-associated protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013949">
        <property type="entry name" value="Utp6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056907">
        <property type="entry name" value="UTP6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055347">
        <property type="entry name" value="UTP6_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23271">
        <property type="entry name" value="HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN 66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23271:SF1">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 6 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08640">
        <property type="entry name" value="U3_assoc_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24892">
        <property type="entry name" value="UTP6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="U3 small nucleolar RNA-associated protein 6 N-terminal" evidence="8">
        <location>
            <begin position="9"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="U3 small nucleolar RNA-associated protein 6 homolog C-terminal" evidence="9">
        <location>
            <begin position="348"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="2"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF24892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9689712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="652" mass="75482" checksum="189D455D7D18B49E" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZJM9</accession>
    <name>A0AA38ZJM9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ribosomal RNA-processing protein 17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_012787</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRP17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZJM9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019186">
        <property type="entry name" value="Nucleolar_protein_12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14577">
        <property type="entry name" value="NUCLEOLAR PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14577:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09805">
        <property type="entry name" value="Nop25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="85"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="46"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="95"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="159"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9690306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="202" mass="22967" checksum="60E13969A354C322" modified="2024-01-24" version="1">MEAEVEAEGGQPRPPHAKHIKKRALRNKALSVSFNEKDLRDFVGGFHKRKKKRRKEAQQKLQESQRIKRIVERKKRKLERELVLYGGAPTATGSEPDECDDEHEQDDGVDEPIASVSGTTMYDNGDAKVIVTTSEISREEENYQTEKTQGAAPPATVGSKKKHNVPVSKKKAFKRVARHRSQPKLQRKRDKKKGGKKNKKIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZJZ1</accession>
    <name>A0AA38ZJZ1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Plant-specific domain TIGR01615 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_012889</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZJZ1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006502">
        <property type="entry name" value="PDDEXK-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01615">
        <property type="entry name" value="A_thal_3542"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31579:SF2">
        <property type="entry name" value="DUF506 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31579">
        <property type="entry name" value="OS03G0796600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04720">
        <property type="entry name" value="PDDEXK_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9690468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="309" mass="34600" checksum="8195F60866A8C433" modified="2024-01-24" version="1">MARATARIPAIHRNFLSANLELGFTAGQAASLSDMAFGFLEDGEGWPESFSSSGGCSENGALDDDEDDDDKEKNSSSVEENKNFWESQHQILHTTLCRTSSLESRIRNATKEALKEIQMDDNVCVCLRPVVGGCPGCLLREVSDRLQNAGYDSAVCKSKWRSSPNIPSGEHTFLDVIHNPNSKKGEVRVIIELNFRAEFEMARASEEYNRLIRRLPEVFVGKVERLHTLVKILCMAAKKCMKEKKMHMGPWRKHRYMQAKWLSTCERSTSITSSLSGDSGRLPKPKASMLTVDLMEKLPNMHCTAVEVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZKB2</accession>
    <name>A0AA38ZKB2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">LPPG:FO 2-phospho-L-lactate transferase CofD/UPF0052</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_013034</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZKB2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043743">
        <property type="term" value="F:LPPG:FO 2-phospho-L-lactate transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07187">
        <property type="entry name" value="YvcK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10680">
        <property type="entry name" value="CofD-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002882">
        <property type="entry name" value="CofD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038136">
        <property type="entry name" value="CofD-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31240">
        <property type="entry name" value="MATERNAL EFFECT EMBRYO ARREST 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31240:SF0">
        <property type="entry name" value="MATERNAL EFFECT EMBRYO ARREST 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01933">
        <property type="entry name" value="CofD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142338">
        <property type="entry name" value="CofD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9690666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="462" mass="50204" checksum="294930E2784322BF" modified="2024-01-24" version="1">MADLGLGPPFSRALPFHFPISPSRFHSAPRSLTMAADHPHSYSFSNPSPIPGPSTSQPSLLVFSGGTAFNGVVEELKKLTTCVAHVLPVSDDGGSTAEIVRVLGGPAVGDIRSRCLRLSDQSTSEALAVRKLLGHRLPLDAQIAKLEWYQIVEGEHALWEGVSKPYRETIRSFLAYFQNQILRRSKESFCFSNGSIGNFFFAGARIFFDSLDAAIFLFSRVSDIPTESLILPVISTNDRLTLGCELWDGSIIRGQNEISHPTSGSMQPINKEISSVPPLPSAIKRVFYMSSEGRNLLHEVFPTANPAVLDQLSNVDCIVYAMGSLFTSICPSLVLLGIGEIISSSSCLKVLLLNGTHDRETSGFSASRFVSAITDALNRTYGDPQNRLKNPPSQYINTLLVPKDGQIPVDVQCLASQGIFHVITVDSICDPDMGIIFDPKSLIRALADLIGRQTTTNVIEAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZNM1</accession>
    <name>A0AA38ZNM1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_011272</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZNM1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003872">
        <property type="entry name" value="Multidrug resistance associated protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF108">
        <property type="entry name" value="ABC TRANSPORTER C FAMILY MEMBER 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="55"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="99"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9692105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="389" mass="43176" checksum="13EBEEA50E2D4D95" modified="2024-01-24" version="1">MRMRSALMVAVYQKQLKLSSLGRRRHSSGQIVNYIAVDAYTTGEFPWWFHSAWSYILQLFLSIGVLLGVVGVGALSGLAPLLICGLLNYESHQVKSWGDKFKNFIESLGDVEFKWLAEAQYKKCYNTVLYWMSPTIVSSVTFLGCALFGSAPLNASTIFTIVAALRCMGEPVRMIPEAISVMIQAKISFERLNAFLLDDELKSEEMRRVNVFGSIAYVSQTSWIQSGTIHDNIQCGKPMDTTKYEKAIKACALDKDINSFDHGDETEIGQRGLNMSGGQKQRIQLARAVYNDADIYLLDDPFSAVDAHTAAILFNDCVIAALQHKTVMLVTHQVEFLSQMEKILVLEGGRVTQSGSYEEHLTTGTAFEQLVNAHKNAITVLDLSNNGGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZNS8</accession>
    <name>A0AA38ZNS8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Band 7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_011204</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the band 7/mec-2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9691992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZNS8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08829">
        <property type="entry name" value="SPFH_paraslipin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.479.30:FF:000004">
        <property type="entry name" value="Putative membrane protease family, stomatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="entry name" value="Band 7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050710">
        <property type="entry name" value="Band7/mec-2_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032435">
        <property type="entry name" value="STML2-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001972">
        <property type="entry name" value="Stomatin_HflK_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43327">
        <property type="entry name" value="STOMATIN-LIKE PROTEIN 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43327:SF10">
        <property type="entry name" value="STOMATIN-LIKE PROTEIN 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16200">
        <property type="entry name" value="Band_7_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00721">
        <property type="entry name" value="STOMATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="Band 7/SPFH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Band 7" evidence="5">
        <location>
            <begin position="67"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="352"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9691992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="414" mass="44666" checksum="01F14477EB56FC19" modified="2024-01-24" version="1">MNLCRAGRSSLNALRASKLVSPHRPISPLLLRHSPILQPPFAAAAVRHLRTGREPPISYEIQPPVNWGVRIVPEKKAYIIERFGKYVKTLESGIHLLIPLVDRIAYVHSLKEEAIPIPDQSAITKDNVSILIDGVLYVKIVDPKLASYGVENPIYAVIQLAQTTMRSELGKITLDKTFEERDTLNEKIVLAINEAAKDWGLKWDISPPRGVRAAMEMQAEAERKKRAQILESEGERQANINIADGNKSSVILESEAAKMDQVNRAQGEAEAILARSQATAKGIEMVSQALKESGGVEAASLRIAEQYIHAFSMIAKEGTTMLLPSTASNPANMIGQALTIYKSLVGNVNGDALPKDSHPGSLGNIKGHTPSGETGAGSPATPRGSDAAPQTREKADTARFGGPGFSLQSSGKVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZNY1</accession>
    <name>A0AA38ZNY1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_011572</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZNY1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016855">
        <property type="term" value="F:racemase and epimerase activity, acting on amino acids and derivatives"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001920">
        <property type="entry name" value="Asp/Glu_race"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21198:SF7">
        <property type="entry name" value="ASPARTATE-GLUTAMATE RACEMASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21198">
        <property type="entry name" value="GLUTAMATE RACEMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53681">
        <property type="entry name" value="Aspartate/glutamate racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9692581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="95" mass="10607" checksum="235068E0B8AEA4A4" modified="2024-01-24" version="1">MGGFEVVVPNRPTMEHTVIPVIESLNRKDMEGARNLLRIALQVLLVRAVNTVILASDDMRDLLPREDPLLKNCIDPTDALARSTINWTRSVEKGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZP67</accession>
    <name>A0AA38ZP67_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Subtilisin-like protease SBT1.5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_011105</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the peptidase S8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9691829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZP67"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02120">
        <property type="entry name" value="PA_subtilisin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04852">
        <property type="entry name" value="Peptidases_S8_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.2310:FF:000002">
        <property type="entry name" value="p69E protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000006">
        <property type="entry name" value="Subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000005">
        <property type="entry name" value="subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.80:FF:000003">
        <property type="entry name" value="Subtilisin-like protease SBT1.9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034197">
        <property type="entry name" value="Peptidases_S8_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010259">
        <property type="entry name" value="S8pro/Inhibitor_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045051">
        <property type="entry name" value="SBT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041469">
        <property type="entry name" value="Subtilisin-like_FN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10795">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17766">
        <property type="entry name" value="fn3_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05922">
        <property type="entry name" value="Inhibitor_I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 10" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 10" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041338391" description="Subtilisin-like protease SBT1.5" evidence="11">
        <location>
            <begin position="28"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="domain" description="Inhibitor I9" evidence="14">
        <location>
            <begin position="35"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="12">
        <location>
            <begin position="144"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="13">
        <location>
            <begin position="385"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="Subtilisin-like protease fibronectin type-III" evidence="15">
        <location>
            <begin position="678"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="9 10">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="9 10">
        <location>
            <position position="226"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="9 10">
        <location>
            <position position="562"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF05922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF17766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9691829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="788" mass="83674" checksum="761F781BFA778677" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZPA8</accession>
    <name>A0AA38ZPA8_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_011523</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZPA8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9692509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="161" mass="18087" checksum="8AD8DCC042964726" modified="2024-01-24" version="1">MCDNSSTIKLSKNPVMHGRSKHIDVRFHFLRDLTKEEVVKLVHCGTNDQVADILTKPLKLEVFLNTGLPKGSYDTHGNMVRHRILGNVELRQTVLILGPRFSHGKFIERPPLGPLLVGSLDPCGGWMVRALHHVPISHVLHSHLLATWLSFISPSLVLFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZRP3</accession>
    <name>A0AA38ZRP3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box/LRR-repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_009623</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9693754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZRP3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38926:SF5">
        <property type="entry name" value="F-BOX AND LEUCINE-RICH REPEAT PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38926">
        <property type="entry name" value="F-BOX DOMAIN CONTAINING PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9693754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="402" mass="45835" checksum="37E520D3AF19236F" modified="2024-01-24" version="1">MAATGVAVHLYWNGTAKTEKYDVVYVGDDMKVMPIKLNYGTTYAGLLDKIYAATGINRKGFELNIICRYPVSSREYKPIPIKNDEAVELMLEVPCRSSRVYCVEIYLEKEARQFALFFFFFFLSFAQMEGRKWEELNMDCLVNVFRRVGMESLLLDVPFVCKSWYKASLDPKCWEHLIFPKYIKPDGIWDTGPLGERLMIQYRESFSVTAFIKSVVARSKRNATLLRLPICCTKEALEYAAIESPTLKTLDLDAVLLFKQSTIIPQLISKWKNLEMLALGSRRNMVEILSQIGLHCNNFIKLFASGIYVGKDEATAMVTSLPNLKCLVLKGSTIEEKNLAMILQGCKELQLLDVRDCIGFWEGNAEILQLASHIPTFMCEGSIYEEYDTFIDGDVDSDYYSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZS99</accession>
    <name>A0AA38ZS99_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RBR-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="6">2.3.2.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_012267</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Might act as an E3 ubiquitin-protein ligase, or as part of E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the RBR family. Ariadne subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="6"/>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9693419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZS99"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22582">
        <property type="entry name" value="BRcat_RBR_unk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22584">
        <property type="entry name" value="Rcat_RBR_unk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1750:FF:000018">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000230">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="105"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="15">
        <location>
            <begin position="109"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="64"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9693419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="314" mass="36348" checksum="AC461C82230DF086" modified="2024-01-24" version="1">MYVPLRVDLKKATSSMFRCPAYKCKAMLELSPGIVPGPVFERWNPAKYEALDIESKKNQSPFEDSNKGGVSRESEYPWNSHKDQLRVLGESGKKVGEPSQGRFRFYGMCEICWERKESTGMFRNEGCSHSVCTDCISKHVEVKIESNSGMILCPGMDCRVVLDPERCKGFLPKTVVERWERAIIETLILDSEKFYCPFKDCSAMLLNDNAEAVMMMRESECPHCRRLFCVQCRAPWHSGMKCREVQRLNADERGREDMLLKKLAEEKKWKRCPQCKFYVEKIDGCVHLTCRCGFQFCYRCGGKWSVNHGTCQRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZSU0</accession>
    <name>A0AA38ZSU0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Alpha/beta hydrolase fold-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_010007</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDXG' lipolytic enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZSU0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050466">
        <property type="entry name" value="Carboxylest/Gibb_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23024">
        <property type="entry name" value="ARYLACETAMIDE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23024:SF139">
        <property type="entry name" value="CARBOXYLESTERASE 15-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="2">
        <location>
            <begin position="85"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9694295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="339" mass="37710" checksum="F27A05810AEAA709" modified="2024-01-24" version="1">MASNNKILEEVSGWLRVYHDNSVDRSWTGPPEVEFLMKPVPSHEDFKDGVATRDVLIDPNTGLSVRIYIPASENGFHVQDKMPLILHFHGGGFCISQADWFMYYHFYAWLVRSVRAVCVSVYLRLAPEHRLPAACDDAYAAFLWLRDVARGEMSESWLKSYADFGRVFLVGDSTGGNIVHDLAARVGGLESEPVRLAGGVAIHPGFLRAEPSKSFLELAESPLLTRDMVNKFMGLALPIGSSKDHPITCPMGAEAPPLAGLKLPPMLVVVAEKDLLRDTELEYCEAMKEAGKDVEVMMNPGMGHSFYFNKLAIEADPETKAQAELLIERIKSFITRQEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZSU6</accession>
    <name>A0AA38ZSU6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_009907</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZSU6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16664">
        <property type="entry name" value="RING-Ubox_PUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000689">
        <property type="entry name" value="RING-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058678">
        <property type="entry name" value="ARM_PUB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057623">
        <property type="entry name" value="PUB12-19-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045210">
        <property type="entry name" value="RING-Ubox_PUB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23315">
        <property type="entry name" value="U BOX DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23315:SF63">
        <property type="entry name" value="U-BOX DOMAIN-CONTAINING PROTEIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25598">
        <property type="entry name" value="ARM_PUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25368">
        <property type="entry name" value="PUB10_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04564">
        <property type="entry name" value="U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51698">
        <property type="entry name" value="U_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="U-box" evidence="10">
        <location>
            <begin position="267"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="7">
        <location>
            <begin position="409"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="655"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="207"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="659"/>
            <end position="670"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9694152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="677" mass="74530" checksum="B81BA6CF95343B96" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZU56</accession>
    <name>A0AA38ZU56_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">ABC transporter B family member 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_010706</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015421">
        <property type="term" value="F:ABC-type oligopeptide transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010329">
        <property type="term" value="F:auxin efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010328">
        <property type="term" value="F:auxin influx transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043481">
        <property type="term" value="P:anthocyanin accumulation in tissues in response to UV light"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009926">
        <property type="term" value="P:auxin polar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090374">
        <property type="term" value="P:oligopeptide export from mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009640">
        <property type="term" value="P:photomorphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009958">
        <property type="term" value="P:positive gravitropism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900459">
        <property type="term" value="P:positive regulation of brassinosteroid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008361">
        <property type="term" value="P:regulation of cell size"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009733">
        <property type="term" value="P:response to auxin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009637">
        <property type="term" value="P:response to blue light"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009741">
        <property type="term" value="P:response to brassinosteroid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048443">
        <property type="term" value="P:stamen development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18577">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18578">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03249">
        <property type="entry name" value="ABC_MTABC3_MDL1_MDL2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000009">
        <property type="entry name" value="ABC transporter B family member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000029">
        <property type="entry name" value="ABC transporter B family member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000066">
        <property type="entry name" value="ABC transporter B family member 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000033">
        <property type="entry name" value="ABC transporter B family member 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394:SF11">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="153"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="233"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="258"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="336"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="368"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="760"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="808"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="884"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="908"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="988"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1026"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="14">
        <location>
            <begin position="110"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="13">
        <location>
            <begin position="432"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="14">
        <location>
            <begin position="764"/>
            <end position="1051"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="13">
        <location>
            <begin position="1087"/>
            <end position="1323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="27"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1334"/>
            <end position="1354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="37"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="50"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1345"/>
            <end position="1354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9695361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1354" mass="148106" checksum="F70DD24AA4E0C4C4" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZV82</accession>
    <name>A0AA38ZV82_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_010421</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZV82"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004158">
        <property type="entry name" value="DUF247_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31170:SF25">
        <property type="entry name" value="BNAA09G04570D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31170">
        <property type="entry name" value="BNAC04G53230D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03140">
        <property type="entry name" value="DUF247"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9694934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="361" mass="40965" checksum="2B0311075A6475C7" modified="2024-01-24" version="1">MEAVDQEKLAPSISMVPIQLRSVKKEAYRPQMVTVGPYHHGNSVLLGMEKHKRHCMQTLLGRVQNPEDTLDDCYMAIKNLNQRVRECYADNMHFGAAELGTILLVDGCFILELFHRHIIQQHTSNSSDPDADNAWMVPTLRHDLELLENQIPFFILVELYKIFQKHLHIFSEYSLTSLALSFFHPNVHVNQEGMSMEPNHLLDLLHKSLLPTPSNDSSGSETRGFDLCASELLQAGIQFEKISESHLLDIKFDSGNGVIKIPPLSIHKTELLLRNLIALEHCGFHNSCKTLHISSYALMMRSLIRSLADVELLEEKGIISSNSVGGRRILCLINDICKNVDIKDFYFGELCKEVNAYCKSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZW60</accession>
    <name>A0AA38ZW60_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Folylpolyglutamate synthase</fullName>
            <ecNumber evidence="4">6.3.2.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Folylpoly-gamma-glutamate synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Tetrahydrofolylpolyglutamate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_010781</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>(6S)-5,6,7,8-tetrahydrofolyl-(gamma-L-Glu)(n) + L-glutamate + ATP = (6S)-5,6,7,8-tetrahydrofolyl-(gamma-L-Glu)(n+1) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10580"/>
            <dbReference type="Rhea" id="RHEA-COMP:14738"/>
            <dbReference type="Rhea" id="RHEA-COMP:14740"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:141005"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a monovalent cation</name>
            <dbReference type="ChEBI" id="CHEBI:60242"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; tetrahydrofolylpolyglutamate biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the folylpolyglutamate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.17" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZW60"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004326">
        <property type="term" value="F:tetrahydrofolylpolyglutamate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.20:FF:000011">
        <property type="entry name" value="Folylpolyglutamate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="entry name" value="Mur ligase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001645">
        <property type="entry name" value="Folylpolyglutamate_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11136:SF5">
        <property type="entry name" value="FOLYLPOLYGLUTAMATE SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11136">
        <property type="entry name" value="FOLYLPOLYGLUTAMATE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="MurD-like peptide ligases, peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0436">Ligase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9695473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="337" mass="37807" checksum="8A843A7F9472EBCD" modified="2024-01-24" version="1">MDVLQQRAHELEVPLEVAAPLDHKNLKGLKLSLSGDHQFINAGLAVSLCKCWLRSTGNWEKVLKNANQEDDLLEAFLRGLSTARLSGRAQTVYDTPPKSYNLSQVTENSFGDLIFYLDGAHSPESMDVCARWFSNAVKERRNSPSSSFVEVGNMVNGYIHHKKENTEESNKISKQILLFNCMEVRDPHILLPQLVRTCASSGTHFSKALFVPSISTYNKVTSGNSIAPLNLPARDLSWQFNLQRIWEKIIHNKDFVVDESFKMDSPGNLPPFEFLYENGSSGSPSSKRLSSSAVMPSLPLTIRWLRDCIKENPSLRLQVLVTGSLHLVGDVLKLLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZWD4</accession>
    <name>A0AA38ZWD4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Potassium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_008587</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Potassium transporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the HAK/KUP transporter (TC 2.A.72.3) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZWD4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015079">
        <property type="term" value="F:potassium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003855">
        <property type="entry name" value="K+_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053952">
        <property type="entry name" value="K_trans_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053951">
        <property type="entry name" value="K_trans_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00794">
        <property type="entry name" value="kup"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30540">
        <property type="entry name" value="OSMOTIC STRESS POTASSIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30540:SF87">
        <property type="entry name" value="POTASSIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02705">
        <property type="entry name" value="K_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22776">
        <property type="entry name" value="K_trans_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0630">Potassium</keyword>
    <keyword evidence="4 10" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="94"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="184"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="228"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="253"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="300"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="331"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="371"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="428"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="452"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="480"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="509"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="domain" description="K+ potassium transporter integral membrane" evidence="11">
        <location>
            <begin position="60"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="domain" description="K+ potassium transporter C-terminal" evidence="12">
        <location>
            <begin position="560"/>
            <end position="773"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU321113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF22776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9696436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="773" mass="86925" checksum="26DC08C13B4E9C34" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A099</accession>
    <name>A0AA39A099_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_007310</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A099"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051362">
        <property type="entry name" value="WD_repeat_creC_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14107:SF16">
        <property type="entry name" value="AT02583P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14107">
        <property type="entry name" value="WD REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="303"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="338"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="512"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="517"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9698165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="548" mass="58835" checksum="73AD18C5A2862968" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A0E0</accession>
    <name>A0AA39A0E0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycosyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_007622</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="5"/>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A0E0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035251">
        <property type="term" value="F:UDP-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000056">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000080">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050481">
        <property type="entry name" value="UDP-glycosyltransf_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48048">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48048:SF94">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9698646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="481" mass="53218" checksum="3698CBD742D5881C" modified="2024-01-24" version="1">MKKAELVFVPTPGAGHYISAVEFAKRLIHTDDRFSVTILHMRSSLHPHTDPYNTSLLASETHLRIIDLPPVDPPPSHLIHKSVEHYILLFFESFIPHVKDAITHLMSNPDSVPLAGLVLDFFCLPMIDVAKELGLPSYLYLTSGAGFLGLMLYLPTRHSQIGTEFEDSDPDLELRSFVNPVPVRVLPGAVSNKHGGYAAYIKVAQRFREARGIIVNTFSELEPYAVKSFADGQTPPVYTVGPVLDLGGQAHSGSGRVDHSKIMRWLDAQPESSVVFLCFGSMGTFDAPQVREIALGLERSGHRFLWALRLPGPDGKLGGRSDGTKLSEILPEGFLDRIGERGMICEWAPQMEVLGHKSIGGFLSHCGWNSILESIWNSVPMATWPMYAEQQLNAFGLVKELGLAVEMRLDYRQRGGEVVMAEEIDGAIRCVMEHDSMVRKKVKERGEMSRRAVMEGGSSSNSLGRLIADIINHPQFPTNTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A3Q7</accession>
    <name>A0AA39A3Q7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Dienelactone hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_005835</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A3Q7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002925">
        <property type="entry name" value="Dienelactn_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17630">
        <property type="entry name" value="DIENELACTONE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17630:SF52">
        <property type="entry name" value="ENDO-1,3-1,4-BETA-D-GLUCANASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01738">
        <property type="entry name" value="DLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dienelactone hydrolase" evidence="2">
        <location>
            <begin position="31"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9700204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="258" mass="27566" checksum="E81C541BFFC93892" modified="2024-01-24" version="1">MSTSRCYENPPSFSPGSGAGTVQEVGGGGLKAYVTGPLDSKLAILFVSDAFGYEAPNLRKLADKVTAAGFLVVAPDFFYGDPVDLSNPNFDRQVWIAAHGTDKGCEDAKAEIAALKSKGVSAIGAAGFCWGGKVVVNLASSDHIQAAVVLHPGRITDDEINDKEVKTPITILGAEIDDASPPEQLKLFGEILSAKSGMDCFVKIFPGVAHGWTVRYSVEDEWGVKSAEEAHGDVLNWFSKYTKGEMENEPQISRRINL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A7T9</accession>
    <name>A0AA39A7T9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SPX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_004352</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A7T9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016036">
        <property type="term" value="P:cellular response to phosphate starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14481">
        <property type="entry name" value="SPX_AtSPX1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031142">
        <property type="entry name" value="SPX_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45978:SF3">
        <property type="entry name" value="SPX DOMAIN-CONTAINING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45978">
        <property type="entry name" value="SPX DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03105">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SPX" evidence="2">
        <location>
            <begin position="1"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="189"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="190"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="201"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9702573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="284" mass="32658" checksum="12FB88C58E5E426F" modified="2024-01-24" version="1">MKFWKSLSNLIEETLPAWRDKFLSYKDLKKQLKLIYPKVDDAHPNKRARSDGGGGEASDVTKEVTDFVRLLEDEIEKFNIFFVEKEEEYVIKLKELQDGVAKMDSNEELMKIGREIVDFHGEMILLENYSALNYTGLVKILKKYDKRSGALIRLPFIQKVLQEPFFTTDLLYKLVKECETMLDHLFSMNKEPSASSSKGTKGKEPNDPDTTTESKERQLKVPKELAEIEHMESMYVKLTLSALRVLKEIRSGSSTVSTFSLPPLQTNSMEETWKKSTVLEQAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A7Z4</accession>
    <name>A0AA39A7Z4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">NB-ARC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_006801</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A7Z4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF955">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0611">Plant defense</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NB-ARC" evidence="4">
        <location>
            <begin position="361"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="5">
        <location>
            <begin position="602"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="6">
        <location>
            <begin position="764"/>
            <end position="1050"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="322"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="325"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9701578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1075" mass="123344" checksum="FB93EAF97AAA9924" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AA34</accession>
    <name>A0AA39AA34_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_004408</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AA34"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000223">
        <property type="entry name" value="probable receptor-like protein kinase At1g80640"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000342">
        <property type="entry name" value="Protein kinase superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47989">
        <property type="entry name" value="OS01G0750732 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47989:SF27">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041349780" description="Protein kinase domain-containing protein" evidence="9">
        <location>
            <begin position="17"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="75"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="155"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9702658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="455" mass="49559" checksum="BC4309447BEE648E" modified="2024-01-24" version="1">MKFFLLILPLLQSIWSFHFSIVEAISDPPLSYISAPLSSPPTGATSPISSSMAAFSPAIQEGSKENQMDSHKKTFIALIVASAALGVTILILLSLWIHKKCSQKSHKSNAQSSDAEKALTLGPLLAKFNTMKVIGTKGSVSLIDYKVLETATNNFQESNILGEGGFGCVYKARLDDNSHVAVKKIDGRGQDAEREFENEVDLLTKIQHPNIISLLGYSSHEESKFLVYELMQNGSLETQLHGPSHGSSLTWHIRMKIALDAARGLEYLHEHCNPPVIHRDLKSSNILLDSNFNAKLSDFGLAVIDGPQNKNNLTLSGTLGYLAPEYLLDGKLTDKSDVYAFGVVLLELLLGRKPVEKLAPAQCQSIVTWAMPQLTDRSKLPGIVDPVIRDTMDLKHLYQVAAVAVLCVQPEPSYRPLITDVLHSLIPLVPVELGGMLKVTQRAPPINTTVPSAGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AAK9</accession>
    <name>A0AA39AAK9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_005282</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AAK9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009809">
        <property type="term" value="P:lignin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05283">
        <property type="entry name" value="CAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000022">
        <property type="entry name" value="Cinnamyl alcohol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.180.10:FF:000004">
        <property type="entry name" value="probable cinnamyl alcohol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.180.10:FF:000100">
        <property type="entry name" value="Putative cinnamyl alcohol dehydrogenase 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047109">
        <property type="entry name" value="CAD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42683">
        <property type="entry name" value="ALDEHYDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="7">
        <location>
            <begin position="23"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9703936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="359" mass="39042" checksum="A4AC2D4D8A8C4AEB" modified="2024-01-24" version="1">MAKSPQEEHPQKAFGWAARDPSGILSPFHFSRRENSPDDVTVRVLFCGVCHTDLHSIKNDWGFTKYPIIPGHEFTGIVTKLGKNVKKFKLGDKVGVGVMVGSCRSCESCKQGLENYCPKMMFAYNSIYHDGTKNYGGYSNMVVVDQHFVLRFPDNLPLAAGAPLLCAGITVYSPMKYYGMTQAGKHLGVSGLGGLGHVAVKIGKAFGLKVTVISASPNKKDDAIKRLGSDAFLLSTDPAKMKAALGTMDYIIDTVAAVHPLGPLLGLLKVNGKLITLGLPEKPLDLPIFPLVLGRKLVGGSNIGSMKETQEMLEFCAKHNITADIEMIRMDYINTAMERLAKSDVRYRFVIDVAGSLSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ACB3</accession>
    <name>A0AA39ACB3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Alpha/beta hydrolase fold-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_003175</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDXG' lipolytic enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ACB3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050466">
        <property type="entry name" value="Carboxylest/Gibb_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033140">
        <property type="entry name" value="Lipase_GDXG_put_SER_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23024:SF257">
        <property type="entry name" value="ALPHA_BETA HYDROLASE FOLD-3 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23024">
        <property type="entry name" value="ARYLACETAMIDE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01174">
        <property type="entry name" value="LIPASE_GDXG_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="3">
        <location>
            <begin position="75"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9705003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="320" mass="35052" checksum="58A29A34436A1301" modified="2024-01-24" version="1">MDPKATEIAHDLPPFLRAYTDGRVERFFGTDVVPPSVDSETGVSTKDVAIAPERGVSARIFKPNTINPDQKLPLLIYYHGGALCLGSPYCTTYHNYVTSLVDEANIIAVSVDYRLAPEHPVPVPYEDSWAATQWVVSHSHGQGPEAWLNDHSDFKRVFLAGDSAGANIAHNMAIRAGVEGLGVVKLSGICLVHPYFGRREADCDSRGTGDSLVDKKPGVDNKWLFVCPNTSGINDPIINPAADQNLRKLGCSKVLICVAEKDGLRKRGWFYYEVLGKSGWGGALEIVETEGEDHVFYLFKPGCEKAVALMKRLASFMNQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AD55</accession>
    <name>A0AA39AD55_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">tRNA uridine 5-carboxymethylaminomethyl modification enzyme C-terminal subdomain domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_005433</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MnmG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9704144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AD55"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070899">
        <property type="term" value="P:mitochondrial tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.570:FF:000001">
        <property type="entry name" value="tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000094">
        <property type="entry name" value="tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000119">
        <property type="entry name" value="tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.570">
        <property type="entry name" value="GidA associated domain, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00129">
        <property type="entry name" value="MnmG_GidA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004416">
        <property type="entry name" value="MnmG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002218">
        <property type="entry name" value="MnmG-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020595">
        <property type="entry name" value="MnmG-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026904">
        <property type="entry name" value="MnmG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047001">
        <property type="entry name" value="MnmG_C_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044920">
        <property type="entry name" value="MnmG_C_subdom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040131">
        <property type="entry name" value="MnmG_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00136">
        <property type="entry name" value="mnmG_gidA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11806">
        <property type="entry name" value="GLUCOSE INHIBITED DIVISION PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11806:SF0">
        <property type="entry name" value="PROTEIN MTO1 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01134">
        <property type="entry name" value="GIDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13932">
        <property type="entry name" value="SAM_GIDA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SMART" id="SM01228">
        <property type="entry name" value="GIDA_assoc_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01280">
        <property type="entry name" value="GIDA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA uridine 5-carboxymethylaminomethyl modification enzyme C-terminal subdomain" evidence="8">
        <location>
            <begin position="532"/>
            <end position="606"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9704144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="649" mass="72953" checksum="143582A5B645141A" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ADG5</accession>
    <name>A0AA39ADG5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">procollagen-proline 4-dioxygenase</fullName>
            <ecNumber evidence="4">1.14.11.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_003319</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-prolyl-[collagen] + 2-oxoglutarate + O2 = trans-4-hydroxy-L-prolyl-[collagen] + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:18945"/>
            <dbReference type="Rhea" id="RHEA-COMP:11676"/>
            <dbReference type="Rhea" id="RHEA-COMP:11680"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:50342"/>
            <dbReference type="ChEBI" id="CHEBI:61965"/>
            <dbReference type="EC" id="1.14.11.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the P4HA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.2" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ADG5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004656">
        <property type="term" value="F:procollagen-proline 4-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.620:FF:000002">
        <property type="entry name" value="Prolyl 4-hydroxylase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045054">
        <property type="entry name" value="P4HA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044862">
        <property type="entry name" value="Pro_4_hyd_alph_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869:SF195">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13640">
        <property type="entry name" value="2OG-FeII_Oxy_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00702">
        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0408">Iron</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="16">
        <location>
            <begin position="159"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9705199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="284" mass="31891" checksum="8B6710A85235A041" modified="2024-01-24" version="1">MKGKGKGVWRPKLGLLLLFISWSFFFLAGLFGSMLFSQDVDGVRSQPRLLESVEEYSPMPHGETGESSVDLIPFQVLSWKPRALYFPRFATAEQCQSIIEMAKSNLRPSTLALRQGETDESTKGTRTSSGTFISSLEDKTGILDFVERKIAKATMIPRSHGEAFNILRYEIGQRYNSHYDAFNPAEYGPQTSQRVASFLLYLSDVEEGGETMFPFEHDLNIGTGYDYKKCIGLKVKPQRGDGLLFYSVFPNGTIDPTSLHGSCPVIAGEKWVATKWIRNEQQDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AEL1</accession>
    <name>A0AA39AEL1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_003106</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AEL1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046246">
        <property type="term" value="P:terpene biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050651">
        <property type="entry name" value="Plant_Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47947">
        <property type="entry name" value="CYTOCHROME P450 82C3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47947:SF8">
        <property type="entry name" value="CYTOCHROME P450 82C4-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0349">Heme</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="135"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9704894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="218" mass="24446" checksum="37D0243F3213CB00" modified="2024-01-24" version="1">MADKYGPIFCFLAKKCYTTSDKVFATRPRSLAIKLMGYDHGSFVFAPYGPYWCDVRKLAMVELLSNRQLEMHKHSFMGNGQWASNKDGPALAEMKERFGNLSLNVVVRAISGKPYFGTHACGDEPKRGKKAFDDFMILVGLFMVSDAIPFLGWLDTVKGFTARMKRVAKEEHRQNRLSANDNGAEQDFIHAMLSVIDDGQFSGRDPETIIKGTCSNLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AGH1</accession>
    <name>A0AA39AGH1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Replication factor C subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_002270</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AGH1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005663">
        <property type="term" value="C:DNA replication factor C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050238">
        <property type="entry name" value="DNA_Rep/Repair_Clamp_Loader"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669:SF52">
        <property type="entry name" value="OS10G0574500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669">
        <property type="entry name" value="REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="post-AAA+ oligomerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="9"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9707196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="742" mass="83318" checksum="6AA043C3D36B183F" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AGS6</accession>
    <name>A0AA39AGS6_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_002387</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Endomembrane system</location>
            <topology evidence="4">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AGS6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001447">
        <property type="entry name" value="Ras-related protein Rab-1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00037868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9707361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="172" mass="19762" checksum="8F40BBF155895114" modified="2024-01-24" version="1">MSNNYDYLFKFLLIGDSSVKKLCLFLRFADDSYVDSYTSTIGVDFKIRTIWDIAGQEQFKTITSSYYRGAHGIINVYNVTEMESFNNVKQWLNEIDRYVNDSVCKLLVGNKCDLVEKKVGDTKIAKAFADELGIPFLETSAKDSINVEMYSPNASNHQMGCSIFWKFQFLEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AHB4</accession>
    <name>A0AA39AHB4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PVL29_002509</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the RING-type zinc finger family. ATL subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AHB4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16461">
        <property type="entry name" value="RING-H2_EL5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45768:SF16">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE ATL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45768">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF13-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="17">
        <location>
            <begin position="94"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="191"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="203"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ9707506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="271" mass="30159" checksum="E708879196661E0B" modified="2024-01-24" version="1">MTYALSPPPTGFPGILPDEGVIRESSSSLSSKDGFSTSNIVTVILILSSLFFVSITLYLLLRYLNRRYLSLNESLPLFTFGSIRGRNSSSTDDCAVCLSKFEPHDQLRLLLICGHAFHARYIDTWLASNQTCPLCRYPIFATEADFMKAILASTNDGDSFRIELHFVKARRSYSVGSFDYIVDHQSEVTVASTHRRGGSESSAAEKNKDDARAQLSSTASISTHALSFRSSSRFFTGSGRRNEAANINDWDLEANRVGEEISKMFRWFSGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AJD2</accession>
    <name>A0AA39AJD2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_000250</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AJD2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001119">
        <property type="entry name" value="SLH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33740">
        <property type="entry name" value="GPI-ANCHORED ADHESIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33740:SF1">
        <property type="entry name" value="SLH DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00395">
        <property type="entry name" value="SLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51272">
        <property type="entry name" value="SLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="90"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="SLH" evidence="3">
        <location>
            <begin position="249"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="476"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9708095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="579" mass="65386" checksum="95110371EB7B60A8" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AM63</accession>
    <name>A0AA39AM63_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_000831</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AM63"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9709033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="67" mass="7542" checksum="FDA98A5A2922B580" modified="2024-01-24" version="1">MSQIIGITTAQSVWNSLERMSTAINRARLMELRFMLQTTKKGGMKMMDYILTSQGIIDNLAAIGENF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D4B0</accession>
    <name>A0AA39D4B0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DOG1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_025263</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D4B0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051886">
        <property type="entry name" value="Seed_Dev/Stress_Resp_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025422">
        <property type="entry name" value="TGA_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46354:SF12">
        <property type="entry name" value="DNA-BINDING PROTEIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46354">
        <property type="entry name" value="DOG1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14144">
        <property type="entry name" value="DOG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51806">
        <property type="entry name" value="DOG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DOG1" evidence="1">
        <location>
            <begin position="1"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9671468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="227" mass="25645" checksum="68D2095BD841C9BB" modified="2024-01-24" version="1">MSFHRFYASWFDHLNHLVHQLTLAPKPTTPQDNPALLQLVHKVISHYSQYYRAKSIAAQNDAVSLFTAPWSSSLERSLHWVAGWRPTTVFHLIYTETSARFESRIADILRGVRTGDLVDLSPAQLQRVSELQCETVREENEITSELAKWQEGAVELVEAGGDGNVEKKIGGLMSVLVKADELRMRTIWRVAEMLTPQQAVEFLIAAAELQFGVRVLGLNHDNQRGNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D4T8</accession>
    <name>A0AA39D4T8_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_027228</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9671141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D4T8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045927">
        <property type="term" value="P:positive regulation of growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021864">
        <property type="entry name" value="DUF3475"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007700">
        <property type="entry name" value="DUF668"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045021">
        <property type="entry name" value="PSI1/2/3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31730">
        <property type="entry name" value="OS01G0873900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31730:SF2">
        <property type="entry name" value="PROTEIN PSK SIMULATOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11961">
        <property type="entry name" value="DUF3475"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05003">
        <property type="entry name" value="DUF668"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF3475" evidence="3">
        <location>
            <begin position="165"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="DUF668" evidence="2">
        <location>
            <begin position="384"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="525"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="536"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="604"/>
            <end position="613"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF11961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9671141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="651" mass="72003" checksum="12307693D5F9E373" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D4Y5</accession>
    <name>A0AA39D4Y5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_027057</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D4Y5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35463">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35463:SF10">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9670884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="163" mass="17983" checksum="01D87D3B318FE13B" modified="2024-01-24" version="1">MDRFRRLTFSTAWFLFFFFIIFYPQSLCSDNVADGHGGQNNAETNEDEKPSTFVMLTDTLALLSRSQISTWNKVKSFLNQLQLHFSPPNLDFRGRDIGQSRMGEGDGPGGRMKEAAEESAKSAVAAVDESAKSAAKVVGEAVQMTAEMVKETVSSNQESEAEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D535</accession>
    <name>A0AA39D535_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Agenet domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_025255</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D535"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd20405">
        <property type="entry name" value="Tudor_Agenet_AtDUF_rpt1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20406">
        <property type="entry name" value="Tudor_Agenet_AtDUF_rpt2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008395">
        <property type="entry name" value="Agenet-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014002">
        <property type="entry name" value="Agenet_dom_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31917">
        <property type="entry name" value="AGENET DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31917:SF148">
        <property type="entry name" value="DUF724 DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05641">
        <property type="entry name" value="Agenet"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00743">
        <property type="entry name" value="Agenet"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Agenet" evidence="2">
        <location>
            <begin position="26"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="Agenet" evidence="2">
        <location>
            <begin position="97"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9671456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="160" mass="18518" checksum="35E1E12BD4870452" modified="2024-01-24" version="1">MEIINPQSQSQKPKKNPRNPAKRRKIHQDFDHHVEVCSKEDGFLGSYYEAKVLGKVGRNKLMVEYKTLLSDEDGQQPLKEIVEAADVRPVPPVILVTGFEVLDKLDAFDNDGWWVGRISGSYEDGKYFVYFESSGDELAYPIHRLRVHQEWEDGQWLVVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D5R9</accession>
    <name>A0AA39D5R9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_027105</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10 16">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 16">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the PP2C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D5R9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000004">
        <property type="entry name" value="Probable protein phosphatase 2C 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832:SF839">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C 47-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 12" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="13">
        <location>
            <begin position="93"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9670960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="388" mass="41883" checksum="7AA46B173E814883" modified="2024-01-24" version="1">MGPGTEVSPHTILAEGGGCCKGNVGAAMEDVNDETLDHSNQLNIGKPPRSLSVMRHCISSARLTGAADWESDIGIVSLKSPSSEKSGYLPVLRSGSWSEIGPKQYMEDEYICIDNFHKHFGAAPNIPSPGAFYGVFDGHGGIDAASFTRKNILKFIVEDAHFPVGIKKAVKSAFAKADHAFADASSLDRSSGTTALIALIFGSTMLVANAGDSRAVLGKRGRAVELSKDHKPNCTSERLRIEKLGGVIYDGYLNGQLSVARALGDWHMKGPKGSNCPLSSEPELQEIILTEEDEFLILGCDGLWDVMSSQCAVTMTRKELMSHNDPERCSKELVREALKRNSCDNLTVVVVCFSPDPPPRIEIPRSQRRRSISAEGLDLLKGVLNSNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D669</accession>
    <name>A0AA39D669_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_025265</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D669"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.240.10">
        <property type="entry name" value="At5g01610-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036758">
        <property type="entry name" value="At5g01610-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007493">
        <property type="entry name" value="DUF538"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31676:SF160">
        <property type="entry name" value="OS01G0652700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31676">
        <property type="entry name" value="T31J12.3 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04398">
        <property type="entry name" value="DUF538"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141562">
        <property type="entry name" value="At5g01610-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9671470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="94" mass="11061" checksum="8FAFF2F751DC8126" modified="2024-01-24" version="1">MAISLFQEFRLTSGLLPLANAIEVVFVKSSRYMWIVQQKKVEQHFKKICKLVNYNTEIHSYIEKRRMKKLRGVQAKVPMPWPPVSEITMHDQAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D8Q6</accession>
    <name>A0AA39D8Q6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Purple acid phosphatase</fullName>
            <ecNumber evidence="11">3.1.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_024012</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>a phosphate monoester + H2O = an alcohol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15017"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:67140"/>
            <dbReference type="EC" id="3.1.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.2" evidence="11"/>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D8Q6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00839">
        <property type="entry name" value="MPP_PAPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.380:FF:000001">
        <property type="entry name" value="Fe(3+)-Zn(2+) purple acid phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000034">
        <property type="entry name" value="Fe(3+)-Zn(2+) purple acid phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.380">
        <property type="entry name" value="Purple acid phosphatase-like, N-terminal"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041792">
        <property type="entry name" value="MPP_PAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039331">
        <property type="entry name" value="PAPs-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025733">
        <property type="entry name" value="PAPs_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015914">
        <property type="entry name" value="PAPs_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008963">
        <property type="entry name" value="Purple_acid_Pase-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22953">
        <property type="entry name" value="ACID PHOSPHATASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22953:SF35">
        <property type="entry name" value="FE(3+)-ZN(2+) PURPLE ACID PHOSPHATASE 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14008">
        <property type="entry name" value="Metallophos_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16656">
        <property type="entry name" value="Pur_ac_phosph_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49363">
        <property type="entry name" value="Purple acid phosphatase, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041485562" description="Purple acid phosphatase" evidence="11">
        <location>
            <begin position="27"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="12">
        <location>
            <begin position="57"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9674829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="918" mass="104673" checksum="3896E2649AB81F0A" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DBP9</accession>
    <name>A0AA39DBP9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_022716</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DBP9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000242">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF415">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="44"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="75"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="110"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9677904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="195" mass="21989" checksum="F575292DCA1D098D" modified="2024-01-24" version="1">MQMVGIEPDEISLVSVLPACAQLGALELRKWIHFYAVKAGFLRNICVCNALIEMYAKRGSIEEGRRLFDQMNERDVISWSTMIVGLANHGRAREAIELFQEMQNAKIEPNIITFVSLLSACAHAGLLNEGLRYFESMKRDYNIEPGVKHYGSLVNLLGLSGRLDQALELIKTMPMKLTSPKIAIKCDTHRANFYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DC65</accession>
    <name>A0AA39DC65_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">alanine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_020533</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.7" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DC65"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004813">
        <property type="term" value="F:alanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006419">
        <property type="term" value="P:alanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.980.10:FF:000004">
        <property type="entry name" value="Alanine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.980.10">
        <property type="entry name" value="Threonyl-trna Synthetase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018165">
        <property type="entry name" value="Ala-tRNA-synth_IIc_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050058">
        <property type="entry name" value="Ala-tRNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11777:SF9">
        <property type="entry name" value="ALANINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11777">
        <property type="entry name" value="ALANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="ThrRS/AlaRS common domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50860">
        <property type="entry name" value="AA_TRNA_LIGASE_II_ALA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Alanyl-transfer RNA synthetases family profile" evidence="11">
        <location>
            <begin position="51"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="279"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9678384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="504" mass="56287" checksum="B9074D3C9AAF5C87" modified="2024-01-24" version="1">MGISFSRQKPLDLTKFLVLSPSLQLNFCINPFKYENIFCKQKRVWKISSYDRRKLIAPDHTCTHMLNFALREVLGKHVDQKGSTVLPEKLRFDFSHGKPIHPDHLRRIESIVNDQIEDELDVYRKEATLADAKRINGVRAVFGEFVVDAKVQSIFYTSWFLGLPLPVRVLTIGQKVEDLLADPDNEEWLSVSAELCGGTHISNTREAKAFALLSEEGIAKGIRRITAVTTDRAFKAMELAFSLELEVDAASEAEGSLLEKKVASLRSRVDAAPISAPKKADLRTKISLLQDQVRKEQKKIAEENIQKAVKVATEMADGAASDGKAFCISLVDVGLDTTAVREAVLKVIEQKGISVTVFSTDETTNKAVVYAGVPENGEKFKQLEGCGNKVCINETELIDGTAVFRYNAMQSCLMQKQRERLAMTLVDKEMEFYIDSLTRVDYLSNQRMSVVFCTLDLMPSISLSPFFKEPQFKLCCPTPSQCLPSLDPGIVVPSSRLPKGLKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DD82</accession>
    <name>A0AA39DD82_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Probable Ufm1-specific protease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_022763</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C78 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DD82"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071567">
        <property type="term" value="F:deUFMylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.130:FF:000001">
        <property type="entry name" value="Probable Ufm1-specific protease 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012462">
        <property type="entry name" value="UFSP1/2_DUB_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049387">
        <property type="entry name" value="UFSP2-like_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48153">
        <property type="entry name" value="UFM1-SPECIFIC PROTEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48153:SF2">
        <property type="entry name" value="UFM1-SPECIFIC PROTEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07910">
        <property type="entry name" value="Peptidase_C78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20908">
        <property type="entry name" value="UfSP2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UFSP2 second" evidence="8">
        <location>
            <begin position="267"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="UFSP1/2/DUB catalytic" evidence="7">
        <location>
            <begin position="450"/>
            <end position="639"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00067779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9677977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="647" mass="71585" checksum="82B4E15F37616568" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DD94</accession>
    <name>A0AA39DD94_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ycf20-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_019901</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ycf20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DD94"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007572">
        <property type="entry name" value="Uncharacterised_Ycf20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33787">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33787:SF3">
        <property type="entry name" value="YCF20-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04483">
        <property type="entry name" value="DUF565"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9680723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="207" mass="23436" checksum="D8B8F95D5759FEDF" modified="2024-01-24" version="1">MVASPYHLTHASPKWVSSRYPVVGIVTCLRKTSFGLCFFRVAQPSLFNTSKRMVWSVRSSVDGNRLDPTPTNGTDGRTRLVRVIQGIQIKLSARILELRKDLPMKILFFLVGFYCATAFATVIGQTGDWDILSAALAVFVVELIGALMYRASIHLFDKVRRLITMFNYWKAGLCLGLFLDSFKYEMNNIIGSCNPFNFEIDDFPIFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DHP1</accession>
    <name>A0AA39DHP1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_018469</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DHP1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005162">
        <property type="entry name" value="Retrotrans_gag_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33437">
        <property type="entry name" value="OS06G0361200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33437:SF2">
        <property type="entry name" value="OS06G0361200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03732">
        <property type="entry name" value="Retrotrans_gag"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Retrotransposon gag" evidence="1">
        <location>
            <begin position="99"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9682552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="166" mass="19130" checksum="5F5E5B0E413144B4" modified="2024-01-24" version="1">MQVERQTAESASVASLSIQQLQDMINNTIKAQYGGPLQSTLMYSKPYTKRIDNLHMPVGYQPLKFQSFDGKGNPKQHVAHFIETCNNAGTDGDLLVKQFVHSLRGNAFDWYIDLAPECINNWDQMEREFLNRFYSTQRTVSMMELINTKQWKDEPVVNYINNVGVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DHY9</accession>
    <name>A0AA39DHY9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GDSL esterase/lipase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_017043</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DHY9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46020:SF4">
        <property type="entry name" value="OS04G0650200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46020">
        <property type="entry name" value="OSJNBB0059K02.9 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041417015" description="GDSL esterase/lipase" evidence="5">
        <location>
            <begin position="28"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9684866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="343" mass="38435" checksum="6D70BF33CF728299" modified="2024-01-24" version="1">MEKESHFFIVCFCLFICFKTIATEAHAHGFTKLFVFGDSYVDTGNRNFSARSWNRPYGITLPGKPAGHYSDGRVFSDYIASWMGIRSPTPYRWRKRENTSLEYGMNFAFGGTGVFDTLVSAPNMTIQIDLFQRQLQKKLYTKVDLNSSIALVSLAGNDYTAYLARNGTTEGLSAFTKSMIKQLGLNLQRIHGLGVRKIAVMNIQPLGCLPSETISDLYKNCSTSGNSVALFHNQVLEKTVEKLNNKSKESVSVILNMYKAFLSALKRQASQPGTLKFKDPLRPCCEAVSTGYKCGNSEHGKRMYVVCNNPNSTFFWDDVHPSQAGWRAVYLALKSSAYSHFLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DMJ3</accession>
    <name>A0AA39DMJ3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">glucose-6-phosphate 1-epimerase</fullName>
            <ecNumber evidence="3 5">5.1.3.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_014884</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>alpha-D-glucose 6-phosphate = beta-D-glucose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:16249"/>
            <dbReference type="ChEBI" id="CHEBI:58225"/>
            <dbReference type="ChEBI" id="CHEBI:58247"/>
            <dbReference type="EC" id="5.1.3.15"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the glucose-6-phosphate 1-epimerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.15" evidence="3 5"/>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9689409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DMJ3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047938">
        <property type="term" value="F:glucose-6-phosphate 1-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09020">
        <property type="entry name" value="D-hex-6-P-epi_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.98.10:FF:000022">
        <property type="entry name" value="Glucose-6-phosphate 1-epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008183">
        <property type="entry name" value="Aldose_1/G6P_1-epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025532">
        <property type="entry name" value="G6P_1-epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014718">
        <property type="entry name" value="GH-type_carb-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11122">
        <property type="entry name" value="APOSPORY-ASSOCIATED PROTEIN C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11122:SF13">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01263">
        <property type="entry name" value="Aldose_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016020">
        <property type="entry name" value="PHexose_mutarotase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="6">
        <location>
            <position position="167"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="271"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR016020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR016020-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR016020-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9689409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="314" mass="35376" checksum="5BF33C0B194766DB" modified="2024-01-24" version="1">MSSERVPVELCKGINGLEKVVLREVRGSSAEVYLYGGQVTSWKNDHGEELLFVSSKAIFRPPKAIRGGIPICFPQFGNHGSLEQHGFARNRVWSIDVDPPPFPTNTSSRAFIDLILKPSEEDMKIFPHSYEFRLRVALGPGGDLMLTSRIRNTSTEGRPLTFTFAYHTYFSVSDISEVRVEGLETLDYLDNLQNKERFTEQGDAITFESEVDKIYVSTPTKIAILDHEKKRTFVIRKDGLPDAVVWNPWDKKAKAMADFGDDEYKHMLCVEAAAVEKPITLKPGEEWRGRQELSAVPSSYCSGQLDPQKVLQSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DNL7</accession>
    <name>A0AA39DNL7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CASP-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_013529</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3 8">Homodimer and heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Casparian strip membrane proteins (CASP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DNL7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006459">
        <property type="entry name" value="CASP/CASPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006702">
        <property type="entry name" value="CASP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044173">
        <property type="entry name" value="CASPL"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01569">
        <property type="entry name" value="A_tha_TIGR01569"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36488">
        <property type="entry name" value="CASP-LIKE PROTEIN 1U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36488:SF8">
        <property type="entry name" value="CASP-LIKE PROTEIN 1U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04535">
        <property type="entry name" value="CASP_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="81"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="113"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="167"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="Casparian strip membrane protein" evidence="9">
        <location>
            <begin position="27"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9691373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="204" mass="21414" checksum="7A41F45DDECC22F4" modified="2024-01-24" version="1">MASVGEGKPELVGSKQGIVSVTKANRDQIVLVLRVVAFMATASATIVMCLNQETKTLLVGTVGSTPITATLKAKFQHTPAFVFFVVANGLASVHNLVMLVVDVFGGKLDCKGLRLVIITILDMVIVAVVAAGACSAAFMAELGKNGNSHAKWNKLCDKFESFCHQGAGALISSFIALLLLLLISAISIITLHNQKLTSPHATTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DP99</accession>
    <name>A0AA39DP99_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_013082</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DP99"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010227">
        <property type="term" value="P:floral organ abscission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039639">
        <property type="entry name" value="IDA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33599:SF13">
        <property type="entry name" value="FORMIN-LIKE PROTEIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33599">
        <property type="entry name" value="PROTEIN IDA-LIKE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041305479" evidence="5">
        <location>
            <begin position="28"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="66"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9690745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="164" mass="18145" checksum="15ED55AB670F183A" modified="2024-01-24" version="1">MATFFLKQPLSLTLALMCMAMTATVSAALTQRSEEGETSMMKKVQGDRGNIDPTFAAEKLRFYILSKGQTPPSGPSKRHNRRLQFTGGQTRFHALANQQTVPPSHDPSKRDSSTPSEFTRGKLDFQVLPKGQTVPPSGPSKKHYKDISCEKAHTMIIHPECNLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DS65</accession>
    <name>A0AA39DS65_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_010490</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Heterodimer with UBC35 or UBC36.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DS65"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23807">
        <property type="entry name" value="UEV_UBE2V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000019">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 variant 1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBC core" evidence="2">
        <location>
            <begin position="7"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00065118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9695018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="140" mass="16238" checksum="AEE3649683C2B4CA" modified="2024-01-24" version="1">MVIDLFPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQLKLFCDKDYPEKPPSVRFHSRINMTCVNHETGVVEPKKFGILANWQREYTMEDILTQLKKEMAAPQNRKLVQPPEGTYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DV71</accession>
    <name>A0AA39DV71_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Thaumatin-like protein 1b</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_010674</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thaumatin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DV71"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09218">
        <property type="entry name" value="TLP-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.110.10:FF:000002">
        <property type="entry name" value="Thaumatin-like protein 1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.110.10">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037176">
        <property type="entry name" value="Osmotin/thaumatin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001938">
        <property type="entry name" value="Thaumatin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31048">
        <property type="entry name" value="OS03G0233200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00314">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002703">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00347">
        <property type="entry name" value="THAUMATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00205">
        <property type="entry name" value="THN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49870">
        <property type="entry name" value="Osmotin, thaumatin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51367">
        <property type="entry name" value="THAUMATIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041222913" description="Thaumatin-like protein 1b" evidence="4">
        <location>
            <begin position="18"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="26"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="74"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="89"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="144"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="149"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="157"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="177"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="187"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR002703-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9695302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="239" mass="24916" checksum="D34C9975933E7960" modified="2024-01-24" version="1">MCLVTIFNVVCLDGVHSATFTFKNQCPYTVWPGTLTGAGMPQLSSTGFELASGASSSLNVPAPWSGRFWARTLCSTDSTGKFVCATADCGSNNVSCNGAGAIPPASLIELTIAANGGQDFYDVSLVDGFNLPLSVTPQGGSGDCNTTSCPANVNSACPSALAMKGSDGSVIGCKSACLVFNQPQYCCTGQYATPETCKPTDYSRFFKSRCSQAYSYAYDDKSSTFTCTGGPNYLITFCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DVT5</accession>
    <name>A0AA39DVT5_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_009271</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DVT5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="51"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9697375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="77" mass="8336" checksum="E829350202E4E937" modified="2024-01-24" version="1">MQPNQLRTDEGSKEVVSLAGNNPSDLRNGLNGVVSANRIGGSAMEDNSIEVLLEIILLQLIPVLMKGTIVMWMLNCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DWL2</accession>
    <name>A0AA39DWL2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_007314</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Vesicle trafficking protein that functions in the secretory pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Part of the t-SNARE complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the syntaxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DWL2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15841">
        <property type="entry name" value="SNARE_Qc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000037">
        <property type="entry name" value="Putative syntaxin-71-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006012">
        <property type="entry name" value="Syntaxin/epimorphin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF263">
        <property type="entry name" value="SYNTAXIN-72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05739">
        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00914">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="243"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="12">
        <location>
            <begin position="171"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00054128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00061857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9698170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="265" mass="29994" checksum="1DAB4F0334D706F4" modified="2024-01-24" version="1">MSVIDIIFRVDQICKKFDKYDVDKQHNLNAYGDDAFARLYASVEADIESALHKSEIALIETNRAAAVALNAEIRRTKARLLEEVVKLQKLVLKKVKGLTKEELSIRNDLVLALPERIRAIPDGSMAGAKQTANWAASASHKNITFDSSDGNFDSEFFQQSEESSQFRQEYEMRRMKQDQGLDVISEGLDTLKNLANDMNEELDRQVPLIDEIDTKVDKATSDIKNTNVRLKETVTKMRSSQNFCIDIILLCVILGIASYLYNVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DWV0</accession>
    <name>A0AA39DWV0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Rab5-interacting protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_007499</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EMC6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DWV0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097250">
        <property type="term" value="P:mitochondrial respirasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029008">
        <property type="entry name" value="EMC6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010742">
        <property type="entry name" value="RCAF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12906:SF0">
        <property type="entry name" value="GEL COMPLEX SUBUNIT OPTI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12906">
        <property type="entry name" value="PROTEIN C20ORF24 RAB5-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07019">
        <property type="entry name" value="EMC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="109"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9698440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="129" mass="14412" checksum="3415EA14F046773F" modified="2024-01-24" version="1">MKEGKSVKSNGQQQHHHQNGHLSPFKFAKLLDPEASWDKDQLGDVLHWIRQAVALVCGLLWGSIPLVGGIWIILFLLISSGIIYGYYAIVLKIDEEDFGGHGALLQEGLFASITLFLLAWILVYSLVHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DX66</accession>
    <name>A0AA39DX66_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_009176</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DX66"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="344"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9697267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="379" mass="43116" checksum="FA4BFDBEC29AEC0D" modified="2024-01-24" version="1">MKPHLQRFQHCGIENGFNEIPWLGGFNRVNDSDPYLETVGVVGSHLTKFRAESHDAADKNAWGSIDQQPSRVIKLENLQRQVGRYCSKTEARKLMHKRLGDDRMSQKIKPEISDRLGSLILRLALNVDEFNNSVEALLEKDEMSVAFHKLMIMEKCHGSWRIASLMDFFPTPVKLMSPYYPVSSIAISGKSFISFGGFTIWIHWPQASIPFSEDKFKYMKNLDPTNFQPALVSHAPSIWDTEETCDAFSFQKNLVFHQEGLPDMECLLDNPVHRDPFDPYMLDDLILSESLGGSKSLVATVWMHFKIAPPSGRTRKDLHQQLKLEKQSHLQMLKLAVDGSDGLKYEGGDSKRHGHRTSYSSGNGNLENHGAYGVYQFRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DXM3</accession>
    <name>A0AA39DXM3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_006921</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DXM3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080043">
        <property type="term" value="F:quercetin 3-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080044">
        <property type="term" value="F:quercetin 7-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000019">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926">
        <property type="entry name" value="GLUCOSYL/GLUCURONOSYL TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926:SF1560">
        <property type="entry name" value="UDP-GLYCOSYLTRANSFERASE 74E1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9701756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="429" mass="48590" checksum="F277985BD344D275" modified="2024-01-24" version="1">MFQFSKRLASKGLKVTLLITTSSISKSMHAQDSSIDIEIICEGFNQRKAESIEDSLERYRIAASQSLAELIEQRSRSNHPAKILVYDSILPWAQDVAERQGLHGASFFTQSCAVSAIYYHFNQMAFSSPLEGSVVALPSMPLFHVNDLPSFISDKVPDSALLNLLLNQFSNFQKVKWILFNTFTKLEDEVMNWMDSQRPIKTIGPTVPSMYLDKRLEHDRDYGLSLFKQNIDTCITWLDTKEIESVVYVSFGSVASLGEEQMEELAWGLKRSNSHFLWVVKELEEKKFPNNFVEETSGKGLVVSWCPQLEVLAHKAVGCFLTHCGWNSTLEALSLGVPMVAMPQFSDQTTNAKFIEDVWRVGVRVKADEKGIVKRQEIEMCIKEIMEGERGNEMKRNAERWKELAKDAVNEGGSSDKNIEEFVAEILCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DY94</accession>
    <name>A0AA39DY94_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Expansin-like EG45 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_004278</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DY94"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22269">
        <property type="entry name" value="DPBB_EG45-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007112">
        <property type="entry name" value="Expansin/allergen_DPBB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009009">
        <property type="entry name" value="RlpA-like_DPBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47480">
        <property type="entry name" value="EG45-LIKE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47480:SF1">
        <property type="entry name" value="EG45-LIKE DOMAIN CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03330">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50842">
        <property type="entry name" value="EXPANSIN_EG45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041369782" description="Expansin-like EG45 domain-containing protein" evidence="2">
        <location>
            <begin position="23"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like EG45" evidence="3">
        <location>
            <begin position="70"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9702461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="159" mass="17290" checksum="9FFD8175022618B3" modified="2024-01-24" version="1">MLERKSEHLVFIVLGLFHVCSGDIGTAGQYAPPYLRKHQRPIPVNQSFETDTVFPFLFFFFFFNLGIDAATACYGNDVSKFPSNNLFASAGDGIWDNGAACGREYFVKCLSAQTPGTCKAGQIIKVKVVDRASRNGVVLVLSTIAFGVIADPSVAWVTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DZC1</accession>
    <name>A0AA39DZC1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DDE Tnp4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_006348</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HARBI1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DZC1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045249">
        <property type="entry name" value="HARBI1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930:SF265">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN, HARBINGER TRANSPOSASE-DERIVED NUCLEASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE Tnp4" evidence="8">
        <location>
            <begin position="2"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9700975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="182" mass="21201" checksum="1630AD0821ACC8D0" modified="2024-01-24" version="1">MAVCDFDMIFRFVIVGWEGTTHDSRVLTKPFRNPQHNFPMPPSGFMAPYRNVRYWLGDFRSGGKAVGKEEIFNQCHARLRNVIEHAFGVVKAHFPILKRMTPYSFTTQTKIVMACFSIHNFLRQISIADRLFSEYDNEVELESDNANQNQNSTTSSFFAAFDQEFMQQFRDQIANELFQVFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E1J6</accession>
    <name>A0AA39E1J6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_004874</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E1J6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02205">
        <property type="entry name" value="CBS_pair_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050511">
        <property type="entry name" value="AMPK_gamma/SDS23_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780">
        <property type="entry name" value="AMP-ACTIVATED PROTEIN KINASE, GAMMA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780:SF46">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0129">CBS domain</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CBS" evidence="5">
        <location>
            <begin position="347"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="167"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9703255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="426" mass="45933" checksum="D8DC8C3A32A16A4B" modified="2024-01-24" version="1">MASVFLYHVVGDLTVGKPELVEFPETETVESAIRVIGESAEGSIPIWKRRSHVGVMENSEMRRQRFVGILNSLDIVAFLARDACLVDQEKAMKTPVSEVVVPNNSLLREVDPATRLIDALEMMKHGLKRLLVPKSVVWKGMSKRFSILYNGKWLKNLDASSSSTNLAPNANRPSSSSTPSSRNKFCCLSREDVIRFVIGCLGALAPLPLSSISSLGAISPNYYSIEASFPAIQVTQKLPQDPSAVAVVESTPDGQYKIIGEISACKLWKCDYLAAAWALANLSAGQFVMGVEDNVTSRSLPHFSVNSAGENNMANGGASTRQRKFSSRSIGFFSNPASPSFGASRSMYRGRSAPLTCKVTSSLAAVMAQMLSHRATHVWVTEADSEDILVGVVGYADILAAVIKQPAPVIPSTTPSGEGYATEIQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E1W7</accession>
    <name>A0AA39E1W7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Kinesin-like calmodulin-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_005459</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. KIN-14 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9704184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E1W7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13200">
        <property type="entry name" value="FERM_C_KCBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01366">
        <property type="entry name" value="KISc_C_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000018">
        <property type="entry name" value="Kinesin-like calmodulin binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000041">
        <property type="entry name" value="Kinesin-like calmodulin-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.530:FF:000005">
        <property type="entry name" value="Kinesin-like calmodulin-binding protein (ZWICHEL)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000131">
        <property type="entry name" value="Kinesin-like calmodulin-binding protein (ZWICHEL)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000090">
        <property type="entry name" value="Kinesin-like calmodulin-binding protein (ZWICHEL)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.530">
        <property type="entry name" value="MyTH4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002404">
        <property type="entry name" value="IRS_PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000857">
        <property type="entry name" value="MyTH4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038185">
        <property type="entry name" value="MyTH4_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011254">
        <property type="entry name" value="Prismane-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47972:SF16">
        <property type="entry name" value="KINESIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47972">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KLP-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02174">
        <property type="entry name" value="IRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00784">
        <property type="entry name" value="MyTH4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21989">
        <property type="entry name" value="RA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00139">
        <property type="entry name" value="MyTH4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56821">
        <property type="entry name" value="Prismane protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51016">
        <property type="entry name" value="MYTH4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="7 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8 11" id="KW-0505">Motor protein</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MyTH4" evidence="16">
        <location>
            <begin position="118"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="FERM" evidence="14">
        <location>
            <begin position="282"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="domain" description="Kinesin motor" evidence="15">
        <location>
            <begin position="892"/>
            <end position="1213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1244"/>
            <end position="1268"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="620"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="749"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="24"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1254"/>
            <end position="1268"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="973"/>
            <end position="980"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00075899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9704184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1268" mass="143574" checksum="36A200FAD83AAFC7" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E2U8</accession>
    <name>A0AA39E2U8_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_001678</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E2U8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="13"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9706268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="127" mass="14440" checksum="36EF8E57A23B5341" modified="2024-01-24" version="1">MPSSMMVRRGHSARPSPQSSPRPRAFPFNVTRISPSKERDKRAVSPNYFPPIRSGSVASRSISPNSSKAKLRNSSEPRSASVFTRTERDRAKDIEQQTSQSKRLFKALISMRKPKKDGTLYRYLDEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E3E4</accession>
    <name>A0AA39E3E4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Copper transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_003656</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the copper transporter (Ctr) (TC 1.A.56) family. SLC31A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E3E4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005375">
        <property type="term" value="F:copper ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007274">
        <property type="entry name" value="Cop_transporter"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04145">
        <property type="entry name" value="Ctr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0186">Copper</keyword>
    <keyword evidence="3 6" id="KW-0187">Copper transport</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="69"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9705684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="126" mass="14068" checksum="77588CD8AFF05C18" modified="2024-01-24" version="1">MAQHGGFWFGVDVDILFTGWPSGYGYFHFYMALVLVYMLSMCAQMFAMTPMTSPKMVPKSLIRHAALHCLRTFITFLVLLCVITFNLGVLITVLLGHVGGYVALTMYIHYHYPAPVADAPANDEKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E3M7</accession>
    <name>A0AA39E3M7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_002250</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E3M7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052426">
        <property type="entry name" value="Plant_dev_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45801:SF5">
        <property type="entry name" value="OS05G0286100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45801">
        <property type="entry name" value="OS07G0101800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="83"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="16"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="113"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="207"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="43"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="119"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9707168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="252" mass="27465" checksum="B7DDE353969F15F1" modified="2024-01-24" version="1">MAAELGLVSLTQLQKLAHSQQHQHPEEENPSSTSSSWMWNPKQAQTQAQAQAQAQEDDDSWEVRAFAEDTGNIMGTTWPPRSYTCTFCRREFRSAQALGGHMNVHRRDRARLHQTHPGSNNPTSSSSTNSSSTLIIPTQEFITNGGLCLLYQLPPNPNALFTPTSINSCLDSPSTLLSIAPYPPNNLISPCPPSINFSAPPQGIALCPSSKPEPSAVSNGDDDNENTSNNYNDSATEELDLELRLGHRPSPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E9J5</accession>
    <name>A0AA39E9J5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NAD-dependent epimerase/dehydratase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_000301</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E9J5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08958">
        <property type="entry name" value="FR_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000085">
        <property type="entry name" value="Dihydroflavonol reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF503">
        <property type="entry name" value="TETRAKETIDE ALPHA-PYRONE REDUCTASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="4">
        <location>
            <begin position="5"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9708172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="320" mass="35391" checksum="442BCB70D3EBEB66" modified="2024-01-24" version="1">MANFCVTGGTGFIAAYLVKRLLEDGHCVRTTVRDPGDVEKVGFLWELSGAKERLKIMKADLMVEGSFDEAVQGVDGVFHTASPVLVPYDENIKATLIDPCINGTLNVLRSCSKSSSLKRVVLTSSCSSIRYRDDVQQVSPLNESHWSDPEYCKRNNLWYAYAKTIAEKEAWKLAKENGIDLVVVNPSFVVGPLLARQPTSTLLMILSVVKGLRGEYPNTTVGFVHIDDVIAAHMLAMEESKASGRLVCSSSVAHWSQIIDMLRDKYPAYPFESKCSSSEGDNHPHSMDTSKIIQLGLPPFKTLAQMFDDCIKSFQDKGFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YG96</accession>
    <name>A0AA38YG96_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Omega-hydroxypalmitate O-feruloyl transferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_026459</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9669900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YG96"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050317">
        <property type="entry name" value="Plant_Fungal_Acyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31642:SF189">
        <property type="entry name" value="ACYLTRANSFERASE GLAUCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31642">
        <property type="entry name" value="TRICHOTHECENE 3-O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9669900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="447" mass="49785" checksum="7CD424C01F0976E0" modified="2024-01-24" version="1">MGTIYQDPPPLIQDLKVTVHSSSLVLPSSETERKSMFLSNIDKVLNFNVQTVHFFQAHPDFPPPTVVEKLETALQKILVPYDFLAGRLKMNPKLDRLEVDCNAAGAGFVVASSEFTLDEIGDLVYPNPAFQQLVVMSMDNLAPDDQPLCILQVTSFKCGGFVMGVTNNHATFDGLSFKMFLENLASLAADKPLVITPCNDRQLLAARSPPRVTFSHPELLKLKIPIGEESIAPAFDATPEELDFKIFRLMPGDISNLKAKAKTNDAKTRVTGFNVVATHIWRCKALSCDTGNDLDRLSTILYAVNIRPRLVPPLPPSYTGNGVLTAYATASCRDLQEKPFSRLVGMVAEGANRMTDEYARSAIDWGELYKGFPHGEFLISSWWRLGFAEVEYPWGKAKYSCPVVYHRKDIILFFPDIDADNNGVNILVALPSKEMEKFQTLFRKFLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YGK9</accession>
    <name>A0AA38YGK9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein SCO1 homolog 2, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_026552</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SCO1/2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YGK9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02968">
        <property type="entry name" value="SCO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000013">
        <property type="entry name" value="Blast:Protein SCO1 homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003782">
        <property type="entry name" value="SCO1/SenC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12151">
        <property type="entry name" value="ELECTRON TRANSPORT PROTIN SCO1/SENC FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12151:SF1">
        <property type="entry name" value="PROTEIN SCO1 HOMOLOG 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02630">
        <property type="entry name" value="SCO1-SenC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9670064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="269" mass="30311" checksum="437A2C7390298F89" modified="2024-01-24" version="1">MSLSRFILSASKKCLIEALNPLQRFGRRFQSRSYSRSSWYGNGRPDIHPPVPVKTQGSQSWSAFVIPAGLLGIAGGALFMHYNDERRAVLKGQGNNSERSAVLGPIIGGPFTLIDAKHQLVTEQNLLGNWVLLYFGYTSSPDVGPEQVQMMAKAIDFLESKQNVRLLPVFVTIDPHRDSPSQLQAYLKEFDSRIVGLTGPDAAIRQMAQEYRVYFRKVEEDGDDYLVESSHNMYLMSPNMEVVRCFGVEYNAEELSQEILKEVKRSITQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YH69</accession>
    <name>A0AA38YH69_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phosphotransferase</fullName>
            <ecNumber evidence="9">2.7.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_026736</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate metabolism; hexose metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the hexokinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.-" evidence="9"/>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YH69"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005536">
        <property type="term" value="F:D-glucose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004396">
        <property type="term" value="F:hexokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001678">
        <property type="term" value="P:intracellular glucose homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000321">
        <property type="entry name" value="Phosphotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.367.20:FF:000003">
        <property type="entry name" value="Phosphotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.367.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001312">
        <property type="entry name" value="Hexokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022673">
        <property type="entry name" value="Hexokinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022672">
        <property type="entry name" value="Hexokinase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19443">
        <property type="entry name" value="HEXOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19443:SF18">
        <property type="entry name" value="HEXOKINASE-LIKE 2 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00349">
        <property type="entry name" value="Hexokinase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03727">
        <property type="entry name" value="Hexokinase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00475">
        <property type="entry name" value="HEXOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51748">
        <property type="entry name" value="HEXOKINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 9" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="6 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Hexokinase N-terminal" evidence="10">
        <location>
            <begin position="41"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="Hexokinase C-terminal" evidence="11">
        <location>
            <begin position="247"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9670379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="489" mass="53841" checksum="34E1615E40669BF4" modified="2024-01-24" version="1">MRKAVVVAAVTTAAMAAAVAVLWSRWKQESERQGRKIRRILRKFARDCATPVSKLWLVADALVSDMNEALTSQETTTLNMLISYAASLPAGDEEGLYYGLNLRGSNFLILRARLRGKNESISELHREEVSIPSNVMGGTSQELFDYIALELAKFTSEHEVNTDDTPDRRKALGFIVSYPVDQAAASSGAAIKWKRFSVTDTAGKALASNFNRALEKHGVNLRVFQLVEDAIGHLAGGRYYNRDTVAAITLGMATTAAYVEPTQAVPEYQSSSNKSSEMVISMDWGNFNSRHLPITEFDTCLDVESSNPGSRIFEKLISGMYLGEIVRRVLLKMAQETALFGEDVPPKLRTPYLLRSPDMAAMHQDTSEDHEVVREKLMEIFRITNSTPLAREVVAEVCDIVAERGARLAGAGIVAITKKLGRIENKRSAVTVEGGLYEHYRVFRNYMHSSVWEMLGNELSDNVVIEHSHGGSGAGAIFVAASQTAEPNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YIX5</accession>
    <name>A0AA38YIX5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Chlorophyll a-b binding protein, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PVL29_025148</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">The light-harvesting complex (LHC) functions as a light receptor, it captures and delivers excitation energy to photosystems with which it is closely associated.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast thylakoid membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the light-harvesting chlorophyll a/b-binding (LHC) protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YIX5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009522">
        <property type="term" value="C:photosystem I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009523">
        <property type="term" value="C:photosystem II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016168">
        <property type="term" value="F:chlorophyll binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009765">
        <property type="term" value="P:photosynthesis, light harvesting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3460.10:FF:000001">
        <property type="entry name" value="Chlorophyll a-b binding protein, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3460.10">
        <property type="entry name" value="Chlorophyll a/b binding protein domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001344">
        <property type="entry name" value="Chloro_AB-bd_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022796">
        <property type="entry name" value="Chloroa_b-bind"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21649">
        <property type="entry name" value="CHLOROPHYLL A/B BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00504">
        <property type="entry name" value="Chloroa_b-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103511">
        <property type="entry name" value="Chlorophyll a-b binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 16 17" id="KW-0148">Chlorophyll</keyword>
    <keyword evidence="4 17" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="12 17" id="KW-0157">Chromophore</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 17" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="9 17" id="KW-0603">Photosystem I</keyword>
    <keyword evidence="15 17" id="KW-0604">Photosystem II</keyword>
    <keyword evidence="6 17" id="KW-0934">Plastid</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13 17" id="KW-0793">Thylakoid</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="binding site" description="axial binding residue" evidence="16">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>chlorophyll b</name>
            <dbReference type="ChEBI" id="CHEBI:61721"/>
            <label>1</label>
        </ligand>
        <ligandPart>
            <name>Mg</name>
            <dbReference type="ChEBI" id="CHEBI:25107"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="16">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>2</label>
        </ligand>
        <ligandPart>
            <name>Mg</name>
            <dbReference type="ChEBI" id="CHEBI:25107"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="16">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>chlorophyll b</name>
            <dbReference type="ChEBI" id="CHEBI:61721"/>
            <label>1</label>
        </ligand>
        <ligandPart>
            <name>Mg</name>
            <dbReference type="ChEBI" id="CHEBI:25107"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="16">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>chlorophyll b</name>
            <dbReference type="ChEBI" id="CHEBI:61721"/>
            <label>1</label>
        </ligand>
        <ligandPart>
            <name>Mg</name>
            <dbReference type="ChEBI" id="CHEBI:25107"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="16">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>chlorophyll b</name>
            <dbReference type="ChEBI" id="CHEBI:61721"/>
            <label>1</label>
        </ligand>
        <ligandPart>
            <name>Mg</name>
            <dbReference type="ChEBI" id="CHEBI:25107"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="16">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>chlorophyll b</name>
            <dbReference type="ChEBI" id="CHEBI:61721"/>
            <label>1</label>
        </ligand>
        <ligandPart>
            <name>Mg</name>
            <dbReference type="ChEBI" id="CHEBI:25107"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="16">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>chlorophyll b</name>
            <dbReference type="ChEBI" id="CHEBI:61721"/>
            <label>1</label>
        </ligand>
        <ligandPart>
            <name>Mg</name>
            <dbReference type="ChEBI" id="CHEBI:25107"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="16">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>4</label>
        </ligand>
        <ligandPart>
            <name>Mg</name>
            <dbReference type="ChEBI" id="CHEBI:25107"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="16">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>chlorophyll b</name>
            <dbReference type="ChEBI" id="CHEBI:61721"/>
            <label>1</label>
        </ligand>
        <ligandPart>
            <name>Mg</name>
            <dbReference type="ChEBI" id="CHEBI:25107"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR601344-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU363080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ9671314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="267" mass="28245" checksum="076BF9DC9C680030" modified="2024-01-24" version="1">MAASTMALSSPSLAGKALKLAPSASDLLGEGRVTMRKTGGRAKPVSSGSPWYGADRVKYLGPFSGEPPSYLTGEFPGDYGWDTAGLSADPETFAKNRELEVIHCRWAMLGALGCVFPELLARNGVKFGEAVWFKAGAQIFSEGGLDYLGNPNLVHAQSILAIWASQVILMGAVEGYRIGGGPLGEVVDPLYPGGSFDPLGLADDPEAFAELKVKEIKNGRLAMFSMFGFFVQAIVTGKGPLENLADHLADPVNNNAWAYATNFVPGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YLG9</accession>
    <name>A0AA38YLG9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">11-oxo-beta-amyrin 30-oxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_026003</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YLG9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050665">
        <property type="entry name" value="Cytochrome_P450_Monooxygen"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24282:SF255">
        <property type="entry name" value="CYTOCHROME P450 72A11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24282">
        <property type="entry name" value="CYTOCHROME P450 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11 12" id="KW-0349">Heme</keyword>
    <keyword evidence="8 11 12" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 12" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="binding site" description="axial binding residue" evidence="11">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9672605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="206" mass="23365" checksum="AC4E909B758087E1" modified="2024-01-24" version="1">MSAKDVIDECKLFYLAGQETTSVLLLWTMVLLSQHPDWQARAREEVLQVFGNNKPENDGLNHLKIVTMIFHEVLRLYPPVTVLTRMISKDTQVGDMYFPAGVQVSLPTILVHHDHEIWGDDAKEFNPERFAEGVSKATKNQVSFLPFGWGPRVCIGQHFAMMEAKIALAMILQRFSFELSPSYSHAPFSLITIQPQYGAHLILRGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YMX3</accession>
    <name>A0AA38YMX3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glutathione transferase</fullName>
            <ecNumber evidence="3">2.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_023160</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GST superfamily. Phi family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YMX3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043295">
        <property type="term" value="F:glutathione binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009407">
        <property type="term" value="P:toxin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03187">
        <property type="entry name" value="GST_C_Phi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03053">
        <property type="entry name" value="GST_N_Phi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000004">
        <property type="entry name" value="Glutathione S-transferase F2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000016">
        <property type="entry name" value="Glutathione S-transferase F2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034347">
        <property type="entry name" value="GST_Phi_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43900">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43900:SF96">
        <property type="entry name" value="GLUTATHIONE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01154">
        <property type="entry name" value="Main.5:_Phi-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0216">Detoxification</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="8">
        <location>
            <begin position="3"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="9">
        <location>
            <begin position="92"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9673429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="223" mass="25157" checksum="E9C84287CC04C228" modified="2024-01-24" version="1">MATPVKVYGPPMSTAVSRVLACLLEKDVNFQLIPVDMSKGEHKKPDYLKIQPFGQVPSFQDESISLFESRAICRYVSEKYANQGNKRLYGTNPLEKASIEQWLEAEGQSFNPPSSALVFQLAFAPRMKLKQDLGVIKQSEEKLAKVLDVYEQRLGESRFLAGDEFTLADLSHLPNTQYLVSVTEKGEMFTKRKNVGRWWSEISGRDSWKKVVALQKAPPPKSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YQ84</accession>
    <name>A0AA38YQ84_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PPPDE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_023912</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DeSI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YQ84"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0101005">
        <property type="term" value="F:deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1720.30">
        <property type="entry name" value="PPPDE domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008580">
        <property type="entry name" value="PPPDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042266">
        <property type="entry name" value="PPPDE_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12378">
        <property type="entry name" value="DESUMOYLATING ISOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12378:SF48">
        <property type="entry name" value="PUTATIVE THIOL PEPTIDASE FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05903">
        <property type="entry name" value="Peptidase_C97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01179">
        <property type="entry name" value="DUF862"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51858">
        <property type="entry name" value="PPPDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPPDE" evidence="4">
        <location>
            <begin position="41"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9674661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="247" mass="28143" checksum="8C47EB57315648D7" modified="2024-01-24" version="1">MKVGLKKGWKSIVPLRLRGKSATRFCIFPKVKSAGYGPGNTPVYLNVYDLTPMNGYVYWAGLGIFHSGVEVHGVEYAFGAHDYPTSGVFEVEPRQCPGFKFRKSILIGTTCLDPIQVREFMERHSASYNGDTYHLIVKNCNHFCKDICYKLTGRPIPKWVNRLAKIGSICNCILPEALKISAVRHDPNYQEYDSEKRRLRSAFNCLSSISMRQRQLSTSSMFLHSPLKGCLPPWELRRSTNRSLKDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YR22</accession>
    <name>A0AA38YR22_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_024110</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YR22"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052276">
        <property type="entry name" value="Diphthamide-biosynth_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44240:SF22">
        <property type="entry name" value="CHAPERONE PROTEIN DNAJ 11, CHLOROPLASTIC-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44240">
        <property type="entry name" value="DNAJ DOMAIN (PROKARYOTIC HEAT SHOCK PROTEIN)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="1">
        <location>
            <begin position="94"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9675002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="203" mass="22256" checksum="7E3E48419ED9B2F4" modified="2024-01-24" version="1">MVKLQPQYVYKLASSLQSSRLSIPSAAITAIQTKQQIPMFSAASFSSSHFVVPKVAVNVRASPPTCVRFRQPKVSASYTATAERTMSHIANPASLYDVLGIQMGATCQEIKAAYRRLARVSHPDVVANSQKDTSADEFIKIHAAYATLSDPEKRADYDRTLFRRRRPVSSPFSMSASAATMGSSSASVFSGHSSLRWETDQCW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YS59</accession>
    <name>A0AA38YS59_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">TOD1/MUCI70 glycosyltransferase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_024509</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YS59"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006852">
        <property type="entry name" value="TOD1_MUCI70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048354">
        <property type="entry name" value="TOD1_MUCI70_glycTrfase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12956">
        <property type="entry name" value="ALKALINE CERAMIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12956:SF24">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN (DUF616)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04765">
        <property type="entry name" value="TOD1_MUCI70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TOD1/MUCI70 glycosyltransferase-like" evidence="1">
        <location>
            <begin position="50"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9675620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="383" mass="44731" checksum="1B194922706C783F" modified="2024-01-24" version="1">MQQSSGLDYFKELHGTSRINLSSSCEMKLLNSTAQLVEPLESRKFARFSLQYTAVEEKPNGQEHWEPRFSGHQSLQEREESFFARDQKINCAFVKSPKGYPSTGFDLAEDDVRYISSCHIAVISCIFGNSDRLRSPAGKTISRLSRKNVCFVMFMDEITLQTLSSERQMPDRMGFIGLWKTVVVKNLPYTDMRRVGKIPKLLAHRLFPSARYSIWLDSKLRLQLDPLLILEYFLWRKGHEYAISNHYDRHCVWEEVAQNKKLNKYNHSIIDQQFAFYQADGLKRFNASDPNKLLPSNVPEGSFIVRAHTPMSNLFSCLWFNEVDRFTPRDQLSFAYTYQKLRRTNPGKPFHLNMFKDCERRAIAKLFRHRSEEKRNILQAAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YSG7</accession>
    <name>A0AA38YSG7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase</fullName>
            <ecNumber evidence="3">3.2.2.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_024689</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>NAD(+) + H2O = ADP-D-ribose + nicotinamide + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16301"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57967"/>
            <dbReference type="EC" id="3.2.2.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:16302"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the disease resistance TIR-NB-LRR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.6" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YSG7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061809">
        <property type="term" value="F:NAD+ nucleosidase activity, cyclic ADP-ribose generating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050832">
        <property type="term" value="P:defense response to fungus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043068">
        <property type="term" value="P:positive regulation of programmed cell death"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10140:FF:000007">
        <property type="entry name" value="Disease resistance protein (TIR-NBS-LRR class)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045344">
        <property type="entry name" value="C-JID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058192">
        <property type="entry name" value="WHD_ROQ1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017:SF570">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN (TIR-NBS CLASS)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20160">
        <property type="entry name" value="C-JID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23282">
        <property type="entry name" value="WHD_ROQ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0611">Plant defense</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="TIR" evidence="14">
        <location>
            <begin position="35"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00061488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9675855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1125" mass="127788" checksum="4F71D99C5CE91521" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YSZ3</accession>
    <name>A0AA38YSZ3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Retrovirus-related Pol polyprotein from transposon RE1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_024893</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9676113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YSZ3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439">
        <property type="entry name" value="GAG-POL-RELATED RETROTRANSPOSON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439:SF440">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9676113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="200" mass="23128" checksum="0E2DF1FA8B86B7F0" modified="2024-01-24" version="1">MVSQFMHVPRPEHFEAVYKILRYLKRTPGRGLLLKSQGHLQIEAYIDTNWVGSIVNRRSTSGYCSFVDSNLVTWRSKKQNVVARSSVEAEFIAVAHGICEIMWIRRLLEELKMTDSSPMKLYCDNKAAISVAHNPVLHDHTKHVEVDKHFIKEKIDNRLVCMTYIPIEEQVADVFTKGLHKRQFNFLVGKLAMEDIFKLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YTZ6</accession>
    <name>A0AA38YTZ6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_021833</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Probable RNA-binding protein that plays a role in meiosis and vegetative growth.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9676502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YTZ6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045927">
        <property type="term" value="P:positive regulation of growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045836">
        <property type="term" value="P:positive regulation of meiotic nuclear division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12524">
        <property type="entry name" value="RRM1_MEI2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12531">
        <property type="entry name" value="RRM3_MEI2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000063">
        <property type="entry name" value="MEI2-like protein 5 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000101">
        <property type="entry name" value="Protein MEI2-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034453">
        <property type="entry name" value="MEI2-like_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034454">
        <property type="entry name" value="MEI2-like_RRM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007201">
        <property type="entry name" value="Mei2-like_Rrm_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23189">
        <property type="entry name" value="RNA RECOGNITION MOTIF-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04059">
        <property type="entry name" value="RRM_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0469">Meiosis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="280"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="365"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="252"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="967"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="257"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="996"/>
            <end position="1021"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00058438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9676502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1032" mass="113948" checksum="A7B3845072D8E6A1" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YVY6</accession>
    <name>A0AA38YVY6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_022487</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YVY6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020847">
        <property type="entry name" value="AP_endonuclease_F1_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF78">
        <property type="entry name" value="OS12G0587133 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00726">
        <property type="entry name" value="AP_NUCLEASE_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="481"/>
            <end position="759"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9677518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1219" mass="140911" checksum="BE06EF7274972AB0" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YYY5</accession>
    <name>A0AA38YYY5_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_021033</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YYY5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027854">
        <property type="entry name" value="STMP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33528">
        <property type="entry name" value="OS07G0239500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33528:SF17">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15054">
        <property type="entry name" value="DUF4535"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9678989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="56" mass="6459" checksum="653DE9D047985F93" modified="2024-01-24" version="1">MGIFRSSFSFMVGTACGIYIAQNYNVPNIKKLANTAIFMAKHLEEKYRKPKKRDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YZJ0</accession>
    <name>A0AA38YZJ0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Neprosin PEP catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_021376</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YZJ0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053168">
        <property type="entry name" value="Glutamic_endopeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004314">
        <property type="entry name" value="Neprosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025521">
        <property type="entry name" value="Neprosin_propep"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31589:SF223">
        <property type="entry name" value="PROTEIN, PUTATIVE (DUF239)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31589">
        <property type="entry name" value="PROTEIN, PUTATIVE (DUF239)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03080">
        <property type="entry name" value="Neprosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14365">
        <property type="entry name" value="Neprosin_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52045">
        <property type="entry name" value="NEPROSIN_PEP_CD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Neprosin PEP catalytic" evidence="1">
        <location>
            <begin position="116"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS52045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9679427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="356" mass="39185" checksum="9038273680DBB7A8" modified="2024-01-24" version="1">MAISEGGVGATAGFEGVSHQHVSPGIGLAEELGRGGGDYSWGARSAQAQDLGGEERRNGGGWLLNRSIHQKTGSASRFKSYPAQMIKPGLVFKTLTEYGDIFDCVDINKQPALDHPLLKNHKVQQAYTRQSPGTYYGAQGGISLHKEPATDHQSHRSAITVSGGSPDKLNAIQVGWTADNFGKTGCRDLLCPGFVQVDKSLAPGMVFQQLSTIDGAQHDYYFSIFQNNSTDENWWLMGWPEKKIIGYWPKTLFPDIKESFTSLEWGGYVQVKDPNTKEYPQMGSGVFPEEGYGKAAYFKFIKLVKNSAGEFQDVSPKEVVTFNDRPTCYRVGQRAELLYWPGYHFFYGGPGGNCGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z0L3</accession>
    <name>A0AA38Z0L3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rhodanese domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_019466</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z0L3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00158">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044684">
        <property type="entry name" value="STR17/STR18/HARC1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44542">
        <property type="entry name" value="THIOSULFATE SULFURTRANSFERASE 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44542:SF12">
        <property type="entry name" value="THIOSULFATE SULFURTRANSFERASE 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="1">
        <location>
            <begin position="25"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9680178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="117" mass="12890" checksum="E91747E56090C9E2" modified="2024-01-24" version="1">MDSQSSATEVVTIDVHAVKDLINLGYRYLDVRAVEEFNKGHPGVENILNIPYLFITPEGRVRNPEFLEHVQSACSKEDHFIVGCQSGVRPFAATSVLVGTSFKDVKEVGKVSKALDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z1H7</accession>
    <name>A0AA38Z1H7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Fungal lipase-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_019773</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z1H7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047714">
        <property type="term" value="F:galactolipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008970">
        <property type="term" value="F:phospholipase A1 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00519">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002921">
        <property type="entry name" value="Fungal_lipase-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31403:SF8">
        <property type="entry name" value="PHOSPHOLIPASE A(1) DAD1, CHLOROPLASTIC-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31403">
        <property type="entry name" value="PHOSPHOLIPASE A1-IBETA2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01764">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4" id="KW-0934">Plastid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Fungal lipase-type" evidence="9">
        <location>
            <begin position="181"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9680554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="418" mass="46560" checksum="435D1A8FF2CFF14D" modified="2024-01-24" version="1">MRLQVRPPCTTRFLPCHSQHGTLTQCVKGMRLTVTNSVVLHQSFESSIVPVNSAKLGKKWMEYQGLNNWDGLLDPLDDTLRNEILRYGKFVEAAYRSFDFDPDSPTYATCKFSRNSLLNRSEIGYTGYKLTKNLHATCGVRLPRWVDRTPAWMSTQSCWIGYVAVCQDKEEIARLGRRDVVIAFRGTATCMEWVENLRATLTSSVGSTNNGGPMVESGFWSLYTSKLPTCPSLQEMVRDEIGRVIRSYGDEPLSITITGHSLGAALATLAAYDIAITFDHAPMVTVVSFGGPRVGNTSFRCQMEKSGTKILRIVNSDDVITKVPGFVIDNNDVAVQAAGLPSWLRKPVEAMQLGYADVGQELRLSSRESPYLKKNNVAACHDLKTYLHLVNGFVSSTCPFRATATRMLERQSREKAFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z1N4</accession>
    <name>A0AA38Z1N4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pectinesterase</fullName>
            <ecNumber evidence="7">3.1.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_019909</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Acts in the modification of cell walls via demethylesterification of cell wall pectin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>[(1-&gt;4)-alpha-D-galacturonosyl methyl ester](n) + n H2O = [(1-&gt;4)-alpha-D-galacturonosyl](n) + n methanol + n H(+)</text>
            <dbReference type="Rhea" id="RHEA:22380"/>
            <dbReference type="Rhea" id="RHEA-COMP:14570"/>
            <dbReference type="Rhea" id="RHEA-COMP:14573"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17790"/>
            <dbReference type="ChEBI" id="CHEBI:140522"/>
            <dbReference type="ChEBI" id="CHEBI:140523"/>
            <dbReference type="EC" id="3.1.1.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Secreted</location>
            <location evidence="7">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the pectinesterase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the PMEI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.11" evidence="7"/>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z1N4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004857">
        <property type="term" value="F:enzyme inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030599">
        <property type="term" value="F:pectinesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042545">
        <property type="term" value="P:cell wall modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045490">
        <property type="term" value="P:pectin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd15798">
        <property type="entry name" value="PMEI-like_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.40:FF:000013">
        <property type="entry name" value="Pectinesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000001">
        <property type="entry name" value="Pectinesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.40">
        <property type="entry name" value="Invertase/pectin methylesterase inhibitor family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035513">
        <property type="entry name" value="Invertase/methylesterase_inhib"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033131">
        <property type="entry name" value="Pectinesterase_Asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000070">
        <property type="entry name" value="Pectinesterase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006501">
        <property type="entry name" value="Pectinesterase_inhib_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018040">
        <property type="entry name" value="Pectinesterase_Tyr_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01614">
        <property type="entry name" value="PME_inhib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31707">
        <property type="entry name" value="PECTINESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01095">
        <property type="entry name" value="Pectinesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04043">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00856">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101148">
        <property type="entry name" value="Plant invertase/pectin methylesterase inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00800">
        <property type="entry name" value="PECTINESTERASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00503">
        <property type="entry name" value="PECTINESTERASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0063">Aspartyl esterase</keyword>
    <keyword evidence="7" id="KW-0134">Cell wall</keyword>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041486228" description="Pectinesterase" evidence="7">
        <location>
            <begin position="20"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="domain" description="Pectinesterase inhibitor" evidence="8">
        <location>
            <begin position="20"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9680733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="513" mass="55928" checksum="3D7C0F250C8471EA" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z483</accession>
    <name>A0AA38Z483_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_018145</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z483"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051119">
        <property type="term" value="F:sugar transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17358">
        <property type="entry name" value="MFS_GLUT6_8_Class3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000043">
        <property type="entry name" value="sugar transporter ERD6-like 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044775">
        <property type="entry name" value="MFS_ERD6/Tret1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050549">
        <property type="entry name" value="MFS_Trehalose_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48021">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48021:SF48">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="30"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="66"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="102"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="132"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="161"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="185"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="362"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="395"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="457"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="37"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9682110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="498" mass="54378" checksum="5EB63759D46061DE" modified="2024-01-24" version="1">MERASMEEGLLVEEKTADKYGGIGGSSSSITAAVVFSTAVAVCASFTYGCAAGYTSPAESGIIDDLALSVAEYSFFGSILTIGGILSAAISGKITDLIGRRGTMWFSEIFWTMGWLAIAFAKDHWWLDLGRLSIGFGIGLICYVVPVYIAEITPQNIRGGFTSAHMLMICCGFSLTFFVGTIISWRILALIGAIPCILQVIGLFFIPESPRWLAKVGREEDLVAALRRLRGVNADISQEAAEIQDYTEAFQHLSEARILDLLQRRYAHSLIVGVGLMVLQQFGGSNAIAYYASAIFESADFSSTFGIRAMAILQVLNSYFNRNYILWDFASCIPQTCEHILLQTPVTLLAVFLIDKCGRRPLLMVSAAGMCSSCLVVALSFLLQDLHQWKEITPILVLIGILAYTASFSMGVAGLPWVVMSEIFPINIKGSAGSLVTLSNWFCSWITTYTFNFIFEWSSAGTFLLFSIICGATVLFVAKLLPEIKGRRLEEIQATMIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z5Y1</accession>
    <name>A0AA38Z5Y1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">F-box/FBD/LRR-repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_018831</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z5Y1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053772">
        <property type="entry name" value="At1g61320/At1g61330-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006566">
        <property type="entry name" value="FBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055411">
        <property type="entry name" value="LRR_FXL15/At3g58940/PEG3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34145:SF28">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34145">
        <property type="entry name" value="OS02G0105600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08387">
        <property type="entry name" value="FBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24758">
        <property type="entry name" value="LRR_At5g56370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="13"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="F-box/LRR-repeat protein 15/At3g58940/PEG3-like LRR" evidence="3">
        <location>
            <begin position="105"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="FBD" evidence="2">
        <location>
            <begin position="359"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9683001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="425" mass="49306" checksum="989413663D781217" modified="2024-01-24" version="1">MVMMEKQTRMDRISNLPWDVLDTILVHLPLKDAARTSILSSNWRYKWISLSQFVFDDKCFSNTLLNKVDRWGEIMKIIHRIQENHSGPIDKFELAAYCCPNHLDLDQWMHFLAEKGIKEFVLKEFYFIRRFKLPCCLFSCQQLRHLELYGCRFKLPPEFKGFKCLASLQLRQVCVDSDTLESLILQCPVLERLILLKIDGLTCLKIHNPNLKYLKIDTEFGNIYLENNPLLSSIDIHPTSVNLEMNMFCSLEEWKACNLFRVLGCLSGVKRLSLSGHFLEFLGASNVPERLPERFNRLMALELREVSFKSLNEVMVFLAILQSSPNLEELSISHRDLEGGSYDIPIVEFLKSRYLLGSLNHLQVVRMRGIMVSRPEWELIKLILASSPALKTMTIVKYRGSRIPESVFLQVERASENVKIISLTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z629</accession>
    <name>A0AA38Z629_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_018893</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z629"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010038">
        <property type="term" value="P:response to metal ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.430:FF:000003">
        <property type="entry name" value="FERONIA receptor-like kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.430:FF:000007">
        <property type="entry name" value="FERONIA receptor-like kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000252">
        <property type="entry name" value="Receptor-like protein kinase FERONIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000039">
        <property type="entry name" value="receptor-like protein kinase FERONIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.430">
        <property type="entry name" value="Galactose-binding lectin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045272">
        <property type="entry name" value="ANXUR1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024788">
        <property type="entry name" value="Malectin-like_Carb-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34590">
        <property type="entry name" value="OS03G0124300 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34590:SF15">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12819">
        <property type="entry name" value="Malectin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 15" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041347480" description="Protein kinase domain-containing protein" evidence="15">
        <location>
            <begin position="24"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="428"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="506"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="789"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="790"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="535"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9683088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="856" mass="96015" checksum="9BA2068F9093E5C5" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z8K5</accession>
    <name>A0AA38Z8K5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sugar phosphate transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_016494</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z8K5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004853">
        <property type="entry name" value="Sugar_P_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050186">
        <property type="entry name" value="TPT_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03151">
        <property type="entry name" value="TPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="45"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="99"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="127"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="151"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="220"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="277"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="Sugar phosphate transporter" evidence="6">
        <location>
            <begin position="21"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9684028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="353" mass="38604" checksum="EC73642A448DA7FA" modified="2024-01-24" version="1">MSENKGFELGTIGALSLSVVSSVSIVICNKALISSLGFTFATTLTSWHLLVTFCSLHVALWMKLFEHKPFDAKAVMGFGILNGISIGLLNLSLGFNSVGFYQMTKLAIIPCTVLLETLFFRKKFSRSIQLSLSILLMGVGIATVTDLQLNALGSILSVLAVITTCIAQIMTNTIQKKFKVSSTQLLYQTCPYQALTLFISGPFLDWFLTNQNVFAFKYTLQVLVFIVLSCLISVSVNFSTFLVIGKTSPVTYQVLGHLKTCLVLAFGYVLLHDPFSWRNIIGILIALVGMVLYSYYCTREGQQKTSEASAQSSLQAKEGEADPLINIENETGILTDAAVPMAPVWSSNKDLHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z999</accession>
    <name>A0AA38Z999_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">mannose-1-phosphate guanylyltransferase</fullName>
            <ecNumber evidence="3">2.7.7.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_016703</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; GDP-alpha-D-mannose from alpha-D-mannose 1-phosphate (GTP route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.13" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z999"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004475">
        <property type="term" value="F:mannose-1-phosphate guanylyltransferase (GTP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009298">
        <property type="term" value="P:GDP-mannose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06425">
        <property type="entry name" value="M1P_guanylylT_B_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000013">
        <property type="entry name" value="mannose-1-phosphate guanyltransferase beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056729">
        <property type="entry name" value="GMPPB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045233">
        <property type="entry name" value="GMPPB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050486">
        <property type="entry name" value="Mannose-1P_guanyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22572">
        <property type="entry name" value="SUGAR-1-PHOSPHATE GUANYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25087">
        <property type="entry name" value="GMPPB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="8">
        <location>
            <begin position="2"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="Mannose-1-phosphate guanyltransferase C-terminal" evidence="9">
        <location>
            <begin position="251"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF25087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9684357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="361" mass="39565" checksum="DBF9860FBC32D6DA" modified="2024-01-24" version="1">MKALILVGGFGTRLRPLTLSVPKPLVDFANKPMILHQIEALKAVGVSEVVLAINYQPEVMLNFLKEFETKLGITITCSQETEPLGTAGPLALARDKLIDDSGEPFFVLNSDVISEYPFKEMIEFHKAHGGEASIMVTKVDEPSKYGVVVMEESIGRVDRFVEKPKLFVGNKINAGIYLLNPSVLDRIELRPTSIEKEVFPKIAAEKKLYAMVLPGFWMDIGQPRDYITGLRLYLDSLRKKSSSKLASGAHIVGNVLVDESAKIGEGCLIGPDVAIGPGCVVEAGVRLSRCTVMRGVRIKKHACISSSIIGWHSTVGQWARVENMTILGEDVHVCDEIYSNGGVVLPHKEIKSSILKPEIVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZAJ1</accession>
    <name>A0AA38ZAJ1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Flavin-containing monooxygenase</fullName>
            <ecNumber evidence="5">1.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_017482</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the FMO family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.-.-.-" evidence="5"/>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZAJ1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004499">
        <property type="term" value="F:N,N-dimethylaniline monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020946">
        <property type="entry name" value="Flavin_mOase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050346">
        <property type="entry name" value="FMO-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23023">
        <property type="entry name" value="DIMETHYLANILINE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00743">
        <property type="entry name" value="FMO-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0274">FAD</keyword>
    <keyword evidence="2 5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9685458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="115" mass="13426" checksum="4AE71DA2C361CA35" modified="2024-01-24" version="1">MVILFGYKYHFPFIETNGIVTIDDNRVGPLYKHVFPPRLAPWLSFIGLPKKDTPFMTTELQSKWLVHVLSGKVLLPIEKEMMSNIENYYHHMEETGVPKRFTHALTPNEVLHLFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZBF7</accession>
    <name>A0AA38ZBF7_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_017702</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZBF7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10747">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10719">
        <property type="entry name" value="DnaJ_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.20:FF:000005">
        <property type="entry name" value="Chaperone protein dnaJ 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000058">
        <property type="entry name" value="Chaperone protein dnaJ GFA2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.230.10:FF:000002">
        <property type="entry name" value="Molecular chaperone DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.230.10">
        <property type="entry name" value="Heat shock protein DnaJ, cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.260.20">
        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01152">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012724">
        <property type="entry name" value="DnaJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001305">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02349">
        <property type="entry name" value="DnaJ_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008035">
        <property type="entry name" value="PRK10767.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43096">
        <property type="entry name" value="DNAJ HOMOLOG 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43096:SF52">
        <property type="entry name" value="DNAJ HOMOLOG 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00684">
        <property type="entry name" value="DnaJ_CXXCXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="DnaJ/Hsp40 cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="HSP40/DnaJ peptide-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51188">
        <property type="entry name" value="ZF_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="1 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="J" evidence="7">
        <location>
            <begin position="93"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="CR-type" evidence="8">
        <location>
            <begin position="220"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CR-type" evidence="6">
        <location>
            <begin position="220"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9685760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="451" mass="49075" checksum="2809CA112E871CC6" modified="2024-01-24" version="1">MVRTNGAKLVRCLARKSLQSSLLEASPTPINESLLRGRYRCFGSGICNNSSRVIGIPNSTSVNHRNRLLFGVLNANWGAARWIHGTAYMAANDYYDTLGVSKNASASEIKKAYYGLAKKFHPDTNKEDPDAEKKFQEVQKAYEVLKDEEKRSLYDQVGHDAFEQANTEGGAGNPFHGGGFGNPFEDIFQNDFFKTIFKQNIGGQDVKVSIELSFMEAVQGCSKTVTFQTALPCEACGGSGVPPGTKPETCKRCRGSGMMFMQNGPFRLQSTCTQCGGTGKIVSSFCKSCKGDRVVRGSKSVKLDIMPGVDDNETLKVYRSGGADPEGNQPGDLYVTIRVRQDPVFRREGPDIHVDAVLSITQAILGGTIQVPTLTGDVVLKVRPGTQPGQKVVLKRKGVKGRNSLSFGDQYVHFNVSIPSNLTQRQRQLIEEFAKEEQGEYDKGAAAGASG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZBU4</accession>
    <name>A0AA38ZBU4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Cellulose synthase</fullName>
            <ecNumber evidence="21">2.4.1.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">PVL29_017658</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="24">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17 21">
            <text>[(1-&gt;4)-beta-D-glucosyl](n) + UDP-alpha-D-glucose = [(1-&gt;4)-beta-D-glucosyl](n+1) + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19929"/>
            <dbReference type="Rhea" id="RHEA-COMP:10033"/>
            <dbReference type="Rhea" id="RHEA-COMP:10034"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18246"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.4.1.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="21">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="21">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 21">Glycan metabolism; plant cellulose biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 21">Cell membrane</location>
            <topology evidence="2 21">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 21">Belongs to the glycosyltransferase 2 family. Plant cellulose synthase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.12" evidence="21"/>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZBU4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016760">
        <property type="term" value="F:cellulose synthase (UDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030244">
        <property type="term" value="P:cellulose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16617">
        <property type="entry name" value="mRING-HC-C4C4_CesA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000339">
        <property type="entry name" value="Cellulose synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000009">
        <property type="entry name" value="Cellulose synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005150">
        <property type="entry name" value="Cellulose_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027934">
        <property type="entry name" value="CES_Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13301">
        <property type="entry name" value="X-BOX TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03552">
        <property type="entry name" value="Cellulose_synt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14569">
        <property type="entry name" value="zf-UDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 21" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16 21" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="12 21" id="KW-0135">Cellulose biosynthesis</keyword>
    <keyword evidence="6 21" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="15" id="KW-0464">Manganese</keyword>
    <keyword evidence="14 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 21" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 21" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11 21" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 21" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="810"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="855"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="907"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="936"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="970"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="domain" description="Cellulose synthase RING-type zinc finger" evidence="23">
        <location>
            <begin position="10"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="664"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="22">
        <location>
            <begin position="664"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="674"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="active site" evidence="18">
        <location>
            <position position="394"/>
        </location>
    </feature>
    <feature type="active site" evidence="18">
        <location>
            <position position="781"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="358"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="364"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="365"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="394"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="535"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="536"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="560"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR605150-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR605150-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR605150-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU361116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF14569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KAJ9685707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1009" mass="112498" checksum="295B50C9619C62DC" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZC93</accession>
    <name>A0AA38ZC93_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_015249</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZC93"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36617:SF16">
        <property type="entry name" value="OS04G0516500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36617">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase zinc-binding" evidence="1">
        <location>
            <begin position="192"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9686252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="376" mass="43890" checksum="361A843F59CBA475" modified="2024-01-24" version="1">MEGKAHLVNWEVVCGDKVNGGLGIRKLTLMNRALLGKWAWRFAYDKEALWKQVLGAKHGLEDHGWRTKKAVGASGVGVWKEILKEADWCWDKMGFKVGKGNRIKFWTDVWCGDSALYQRFQQLYILAANRNALVEDLWDQNSGEGGWNLRFIRDFNDWEVEMVGELLQALRDFRIVREDDSVFWKGGGRGQFRVKDAYSWLDRPTDVIFPKNKIWMERVPTKISFFAWEATWGKILTLDRLQKRGWQLPNRCFLCACEAESVNHILIHCTVVKVIWDLVLGLVGVKWVFPNTVKEVLFSWGGAFVGKKRKKLWNSIPCFIFWTVWKERNRLAFRGGDLNIQRLKYSFVCNIWGWAKLYMEEGPHSLLDFLDWLASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZCZ2</accession>
    <name>A0AA38ZCZ2_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_015472</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZCZ2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009717">
        <property type="term" value="P:isoflavonoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000213">
        <property type="entry name" value="Coniferyl alcohol 9-O-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016461">
        <property type="entry name" value="COMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001077">
        <property type="entry name" value="COMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012967">
        <property type="entry name" value="COMT_dimerisation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11746">
        <property type="entry name" value="O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08100">
        <property type="entry name" value="Dimerisation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00891">
        <property type="entry name" value="Methyltransf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005739">
        <property type="entry name" value="O-mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51683">
        <property type="entry name" value="SAM_OMT_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="O-methyltransferase dimerisation" evidence="5">
        <location>
            <begin position="31"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="O-methyltransferase C-terminal" evidence="4">
        <location>
            <begin position="143"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9686614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="344" mass="37517" checksum="9AC9DF258730CA88" modified="2024-01-24" version="1">MVGTSETGDVLKVSSEADEAELMLQGQANIWRHMFAFADSMALKCAVELRIADIIHSHARPITLSQIATCIDSPSPDITCLARIMRFLVRAKIFTAAPPPPQSDGGETLYGLTPSSKWLLHDAELSLAPMVFMENHPSLMAPWHCFGTCVKEGGIAFEKAHGRQIWDFASENPEFNKLFNDGMACTAKISIKAVIAAYKDGFGCIGALVDVGGGTGGAVAELVKAYPHIKGINFDLPHVVATAPAYKGVSHVGGDMFESIPNADAIFMKKTGKVIIVDGVIREDGDEPFDETRLVMDLVMMAHTSHGQERTEDEWKKLLEEGGFPRYRILKIPTLQMIIEAYPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZDE0</accession>
    <name>A0AA38ZDE0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 12">Diacylglycerol kinase</fullName>
            <ecNumber evidence="11 12">2.7.1.107</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_015612</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Phosphorylates the second messenger diacylglycerol (DAG) to generate phosphatidic acid (PA), another important signaling molecule. PA is required for plant development and responses to abiotic stress and pathogen attack. May be involved in the accumulation of PA during cold stress.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11 12">
            <text>a 1,2-diacyl-sn-glycerol + ATP = a 1,2-diacyl-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10272"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.107"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3 11">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11 12">Belongs to the eukaryotic diacylglycerol kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.107" evidence="11 12"/>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZDE0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004143">
        <property type="term" value="F:ATP-dependent diacylglycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.40:FF:000007">
        <property type="entry name" value="diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10330:FF:000016">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037607">
        <property type="entry name" value="DGK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000756">
        <property type="entry name" value="Diacylglycerol_kin_accessory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001206">
        <property type="entry name" value="Diacylglycerol_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016961">
        <property type="entry name" value="Diacylglycerol_kinase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11255">
        <property type="entry name" value="DIACYLGLYCEROL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11255:SF98">
        <property type="entry name" value="DIACYLGLYCEROL KINASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00609">
        <property type="entry name" value="DAGK_acc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00781">
        <property type="entry name" value="DAGK_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF030829">
        <property type="entry name" value="Diacylglycerol_kinase_pln"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00045">
        <property type="entry name" value="DAGKa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00046">
        <property type="entry name" value="DAGKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50146">
        <property type="entry name" value="DAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 11 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 11" id="KW-0611">Plant defense</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 11" id="KW-0346">Stress response</keyword>
    <keyword evidence="4 11 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DAGKc" evidence="14">
        <location>
            <begin position="32"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="435"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="451"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00060336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR030829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9686837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="486" mass="54425" checksum="5CC07CF22E523D2C" modified="2024-01-24" version="1">MAYSNSEFDLKDFRIPDYILVPELEVEAVSYVPECPVIVFINSKSGGQLGGDLLITYRSLLNEKQVFDVNQEAPDKSLSRIYVNLEKLKHKEDDFAFKIQERLRIIVAGGDGTAGWLLGVVSDLKLSQPPPIATVPLGTGNNLPFSFGWGKKNPGTDSRAVESFLGQVKKAKEMKIDSWHILMRMKAPKEGACDPIAPLELPHSLHAFHRVSETDSLNMEGYHTFRGGFWNYFSMGMDAQVSYAFHTERKLHPEKFTNQLVNQGTYARLGCTQGWFAASLFHPASKNIAQVAKISVMKKVGGQWEELKISNSIRSIVCLNLPSFSGGFNPWGTPNAKRRRDRGLTAPFVDDRLIEIVGFRDAWHGLVLLAPKGHGTRLAQAHRIRFEFHKGAADHTFMRIDGEPWKQPLPVDDDTVVVEISHLGQVKMLATDNCKSKSCEHPTPPPAHETEEPDSNEEEEEGEEWKKFGAAETFRIPDDVDISQLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZDY7</accession>
    <name>A0AA38ZDY7_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_015933</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZDY7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9687256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="94" mass="10343" checksum="B3D7ECE4D352AF0A" modified="2024-01-24" version="1">MGREYSGFVSRVGGWSVWGRTHMVASGVAAEEPEQCSVLGLSVDGCTACEHRWGEELGCGRGKERGCDGSRKWSFLRTEMDEAGVCIDIDLRER</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZG97</accession>
    <name>A0AA38ZG97_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Retrovirus-related Pol polyprotein from transposon TNT 1-94</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_016295</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZG97"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9687764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="73" mass="8047" checksum="8C204BA5C5C0578C" modified="2024-01-24" version="1">MGKAKPVSSLLVSHPKLSSKQSPLSEKEKEEMRKVPYALAVGSLMYAMVCTRLDIAHSVGVVSRFFSNPCKEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZGZ0</accession>
    <name>A0AA38ZGZ0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Caffeoyl-CoA O-methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_014111</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-dependent O-methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZGZ0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050362">
        <property type="entry name" value="Cation-dep_OMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002935">
        <property type="entry name" value="SAM_O-MeTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10509:SF82">
        <property type="entry name" value="CAFFEOYL-COA O-METHYLTRANSFERASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10509">
        <property type="entry name" value="O-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01596">
        <property type="entry name" value="Methyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51682">
        <property type="entry name" value="SAM_OMT_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9688228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="242" mass="27230" checksum="44292BD7A6D1512D" modified="2024-01-24" version="1">MGESAKKTTVYKGLLKNEELYQYILETSVYPREPEPLKELRDATAAHPRAFIAAAPDAGQLIDMLLKLVNAKKTIEIGVFTGYSLLLTALSIPNDGKIIAIDVDRKNYEIGLPIIRRAGVEHKINFIESQALPVLDKLLEDHENEGSLDFAFVDADKGNYKNYHERLMKLLKVGGVVVYDNTLWYGTVALPEDLVEELVDGVHMKEMRKHIMQLNKYLATDSRVQICVAPLGDGITICRRIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZHS7</accession>
    <name>A0AA38ZHS7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">HTH myb-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_014281</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZHS7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000002">
        <property type="entry name" value="Myb family transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044787">
        <property type="entry name" value="HHO5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058673">
        <property type="entry name" value="HHO5-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006447">
        <property type="entry name" value="Myb_dom_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01557">
        <property type="entry name" value="myb_SHAQKYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31003">
        <property type="entry name" value="MYB FAMILY TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31003:SF19">
        <property type="entry name" value="MYB FAMILY TRANSCRIPTION FACTOR EFM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26575">
        <property type="entry name" value="HHO5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="8">
        <location>
            <begin position="247"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="230"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="367"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="34"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="234"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="377"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="403"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="433"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9688544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="463" mass="50881" checksum="CCB074F562DE4027" modified="2024-01-24" version="1">MMASPSELSLDCKPHSYSLLLKSFGDQPDQTQKLEDFLARLEEERLKIEVFKRELPLCMQLLTDAVEASRQQLQSYRANQGARPFLEEFIPLKHSTPKGSEKTSNMSDKANWMTSAQLWSPAGDETKQQSTLTSSKEPDIGFTVSPKLGFDNKQRNGGAFLPFSKERNSCPSPNLRALPDLALANSADHKDVEDKKCSETENGIAFSRRENCGKVVNGGATMEQVKAAITPSEAQTTAAAPTANTQTHRKARRCWSPDLHRRFVNALQMLGGSQVATPKQIRELMKVDGLTNDEVKSHLQKYRLHTRRPSPSPQAAGAAAPQLVVLGGIWVPPEYATAAHTGGPTLYGAHPPSHASPHYCGPPVPQDFYAAPAQPHHQLHHHSLHHQLHMYKASPQTQSSPDSDVRGAGDRSESIEDGKSESSSWKGESGENEGERKGLVALRDDGEESNRAMEVRLLSTSNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZIN4</accession>
    <name>A0AA38ZIN4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Hexokinase-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_012367</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9689642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZIN4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36706">
        <property type="entry name" value="UNNAMED PRODUCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9689642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="86" mass="10055" checksum="E52C71B4216C33D4" modified="2024-01-24" version="1">MSSRPVPRRESPWGTPEGEHRQPKPHRCNDRAEDVIQACFEGNPFKTVPGPFKLFWQCMRSKPGEEPTDPFHYLQLDPPKREVQLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZJA0</accession>
    <name>A0AA38ZJA0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Cysteine-rich receptor-like protein kinase 29</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PVL29_012654</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZJA0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23509">
        <property type="entry name" value="Gnk2-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000142">
        <property type="entry name" value="Cysteine-rich receptor-like protein kinase 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.430.20:FF:000002">
        <property type="entry name" value="Cysteine-rich receptor-like protein kinase 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.430.20:FF:000003">
        <property type="entry name" value="Cysteine-rich RLK (RECEPTOR-like protein kinase) 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001959">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.430.20">
        <property type="entry name" value="Gnk2 domain, C-X8-C-X2-C motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002902">
        <property type="entry name" value="GNK2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038408">
        <property type="entry name" value="GNK2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002:SF1073">
        <property type="entry name" value="CYSTEINE-RICH RECEPTOR-LIKE PROTEIN KINASE 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002">
        <property type="entry name" value="RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD1-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01657">
        <property type="entry name" value="Stress-antifung"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51473">
        <property type="entry name" value="GNK2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="12 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 17" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041313926" description="Cysteine-rich receptor-like protein kinase 29" evidence="17">
        <location>
            <begin position="25"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="290"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="19">
        <location>
            <begin position="28"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="19">
        <location>
            <begin position="137"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="350"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="250"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="252"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAJ9690111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="22">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="681" mass="76327" checksum="F463FB0F2862C542" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZJD8</accession>
    <name>A0AA38ZJD8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Chromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_014829</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9689339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZJD8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045358">
        <property type="entry name" value="Ty3_capsid"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19259">
        <property type="entry name" value="Ty3_capsid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chromo" evidence="2">
        <location>
            <begin position="421"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="255"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="394"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="255"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="291"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="398"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9689339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="480" mass="55272" checksum="6E2708B163F3951D" modified="2024-01-24" version="1">MERGLEDLREQIQDLREGMLGSQVQPVSHEEFMSFQDKVMSMFASVESRMEALAAHMEAREQEIRQELAIYKAAVSARVMATQGASRVEVPKPQGFSGKRDAKELDNFLWHMERYFEAIALTDEAAKVRTATLYLTDTATLWWRRRFADMEKGICTIETWEDFKREIKRQFYPEDVAYLVRKNMRRLKHTSSIRDYVKEFSSLMLEIPNMTEEELLFNFMDNLQGWAEQELRRRGVQDLATAMAVAESLTDYKRGDSSKVESLEDSHTTGGGDEASRDHYAPRMGSGKTPNVREGKGKAERKEFTPKIKCFLCDGPHWARDCPKRKALSAMIEEMEQEDEAHMGSMQLLGALQFNPKPSTPKTSLLSGVQVKEVKGERAEVARTHMEAVTKGKVNSMGKRKQHSKHQKRTGLHPSEASREKEVKNILAEQVTRRQGVPPVIEYLVRWKRLPKGQASWEHADALRRFWKHIERFQKEATTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZJY9</accession>
    <name>A0AA38ZJY9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Receptor-like serine/threonine-protein kinase</fullName>
            <ecNumber evidence="15">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">PVL29_012735</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="22">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="15"/>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZJY9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048544">
        <property type="term" value="P:recognition of pollen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00028">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01098">
        <property type="entry name" value="PAN_AP_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.90.10.10:FF:000001">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001019">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase B120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001037">
        <property type="entry name" value="Putative G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.10">
        <property type="entry name" value="Bulb-type lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021820">
        <property type="entry name" value="S-locus_recpt_kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000858">
        <property type="entry name" value="S_locus_glycoprot_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024171">
        <property type="entry name" value="SRK-like_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32444">
        <property type="entry name" value="BULB-TYPE LECTIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32444:SF198">
        <property type="entry name" value="BULB-TYPE LECTIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11883">
        <property type="entry name" value="DUF3403"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08276">
        <property type="entry name" value="PAN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00954">
        <property type="entry name" value="S_locus_glycop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000641">
        <property type="entry name" value="SRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00473">
        <property type="entry name" value="PAN_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 15" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="416"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="20">
        <location>
            <begin position="7"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="19">
        <location>
            <begin position="264"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="21">
        <location>
            <begin position="319"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="485"/>
            <end position="760"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR000641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAJ9690230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="778" mass="87886" checksum="101CAE1AC86C698A" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZNV9</accession>
    <name>A0AA38ZNV9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box/kelch-repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_011473</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZNV9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080037">
        <property type="term" value="P:negative regulation of cytokinin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000762">
        <property type="term" value="P:regulation of phenylpropanoid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044595">
        <property type="entry name" value="KMD1-4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46407">
        <property type="entry name" value="OS02G0208700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46407:SF3">
        <property type="entry name" value="OS02G0208700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9692432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="311" mass="34659" checksum="A6B427D83C2B4CAD" modified="2024-01-24" version="1">MDFIPGLPDDVAGQCLIRSEVEDPDFFRLRKTAGYTRPVFAMAQARVVPNRSSGGMKCPTLAYRVTLLDLETGNWSELPPVPGFSDGLPMFCQLVGVDSELVVVGGWDPDTWEISSSVFIYNFLSATWRRGADMPGARRSFFGCATSGLERVVYVAGGHDAEKNALKSALVYDRDECKGVFHRGKFHVIGGYCTEMQGRFERSAEAFDFANWYTCPRTCVDGGDMGMYMCHAGEVVALQDSRWQTVDKLPAEIRDTAYMTTWQGKLLVIGCRSFGEAHVAYMLDLKSRRWRKLVAPEEFCGHVQSGCCLEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZPJ2</accession>
    <name>A0AA38ZPJ2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Disease resistance protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_011576</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZPJ2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.430:FF:000003">
        <property type="entry name" value="Probable disease resistance protein At5g66910"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050905">
        <property type="entry name" value="Plant_NBS-LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463:SF220">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NB-ARC" evidence="7">
        <location>
            <begin position="160"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="8">
        <location>
            <begin position="413"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="9">
        <location>
            <begin position="554"/>
            <end position="698"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9692585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="905" mass="102884" checksum="A0BB2BE33DB3B8EB" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZQJ2</accession>
    <name>A0AA38ZQJ2_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_012272</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9693426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZQJ2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="162"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="181"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9693426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="213" mass="23057" checksum="F6359F010E2A86A0" modified="2024-01-24" version="1">MLGTILEYYGFPTESDKEKKHHCREVYTVSRWQGSVTKTESTLTSASLTTANEHHYVPPPNLILEDFGDGEDTPPRPPAATTPGATSTPQNPDSAAHILASLSSTPNIGPSASDGYVHISAEAFNQLLARLDMIQENQANIQLTQRQLVQRVDELTMAVQQWNSSSEKPASVHDAPPLSVPPTQETNSTTKESTVPPTILTPSEITPSHPSHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZRN1</accession>
    <name>A0AA38ZRN1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_012044</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FGGY kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9693133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZRN1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004370">
        <property type="term" value="F:glycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006071">
        <property type="term" value="P:glycerol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046167">
        <property type="term" value="P:glycerol-3-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006641">
        <property type="term" value="P:triglyceride metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10196:SF69">
        <property type="entry name" value="GLYCEROL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10196">
        <property type="entry name" value="SUGAR KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9693133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="156" mass="16619" checksum="34BCE861F5D98B10" modified="2024-01-24" version="1">MLGQACQKGEAQSTYGTGAFIFSIQSLEGSIASRWLGDNLGIISIASEIEELAAKVDSSGGVYFVLAFNGLFAPWWHDDARGAKDVLVSMHKDAGEKGEVKNEKGEFLLTVDGSETITNLLMQIQADLLRNPVVRPADIETTAIGAAYATRLAVGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZSJ8</accession>
    <name>A0AA38ZSJ8_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_009913</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ARG7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZSJ8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009733">
        <property type="term" value="P:response to auxin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003676">
        <property type="entry name" value="SAUR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31374">
        <property type="entry name" value="AUXIN-INDUCED PROTEIN-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31374:SF418">
        <property type="entry name" value="AUXIN-RESPONSIVE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02519">
        <property type="entry name" value="Auxin_inducible"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9694160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="94" mass="10591" checksum="1170AFD94F524068" modified="2024-01-24" version="1">MAVISRKRLELAARKLRSRSSCRVPHGYVPMYVGEEHKLYEVPVECLSSVMLQALLIQFEEEIPANGPIALSCSLQMFEWVMGVAMAEKTGHEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZT50</accession>
    <name>A0AA38ZT50_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_010278</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD(P)-dependent epimerase/dehydratase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZT50"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05243">
        <property type="entry name" value="SDR_a5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000253">
        <property type="entry name" value="Uncharacterized protein At5g02240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044163">
        <property type="entry name" value="SARED1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14194">
        <property type="entry name" value="NITROGEN METABOLIC REGULATION PROTEIN NMR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14194:SF86">
        <property type="entry name" value="OS05G0110300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13460">
        <property type="entry name" value="NAD_binding_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-binding" evidence="2">
        <location>
            <begin position="87"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9694727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="329" mass="35996" checksum="330BA1F56C371A42" modified="2024-01-24" version="1">MATATRVPLVSTTSSRLRHCHQSNWVESSFPLRRQPGSYRLFNKSLSSSSSSSSSSFLATPNRRSFRRLGVVVSAAMADLPTVLITGAGGRTGQIVYKKLKERADQFVARGLVRTEESKEKIGGADDVLVGDIRNADSIVPAIQGVDALIILTSAVPRMKPGFDPTKGQRPEFYFEEGAFPEQVDWIGQKNQIDVAKTAGVKHIVLVGSMGGTDPNHPLNSLGNGNILVWKRKAEQYLADSGIPYTIIRAGGLQDREGGIRELLVGKDDELLKTETKTITRNDVAEVCIQALQFEEAKFKAFDLASKPERVGTPTKDFKALFSQITTRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZUI2</accession>
    <name>A0AA38ZUI2_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_010783</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZUI2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34567">
        <property type="entry name" value="FK506-BINDING-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34567:SF3">
        <property type="entry name" value="FK506-BINDING-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="364"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9695476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="396" mass="44457" checksum="C7C940C4C2E20FD1" modified="2024-01-24" version="1">MGGRRQKGDYHHQEFQKTRSLNRKPPLGNWQPTVPSWEKKFCSSVGSFPWQRLLENKRFIYLYDNVLQWNDSAGKEAFHNAKNRFWAQINGLPCDISLPDPDIYIDEIDWNCSIDPEMILDLEQEPVDPDDCVKNEKVSSLGNSLLLLNQSFSCTGWGDAEEDIGKAADIRSGPGLWHHNQNVDNPWELSCTQNKGAVKATGWGDCEEPVKAMGWGDCEEPVEATGWGNSNEPVKATGWGNSNEPVKATGWGDWDQPVEATGWGDYYGAMEGPIWKDGWNNSSGWNQYENKYNDLENLKDRRAGGVWGTGNGNSRGEGGGYMSRYRSSRFNDNEYQADRGWRSGGGKRANFLYDEGPFLDKKAASQPRNTIHHCGPVSHHGSGKAGNSWSWKKPVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZUV5</accession>
    <name>A0AA38ZUV5_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_010310</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the short-chain dehydrogenases/reductases (SDR) family. SDR65C subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZUV5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045000">
        <property type="entry name" value="TR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42898">
        <property type="entry name" value="TROPINONE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42898:SF28">
        <property type="entry name" value="TROPINONE REDUCTASE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9694768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="267" mass="28817" checksum="1CC0AD40923A30CF" modified="2024-01-24" version="1">MAASEPSCRAKRWSLKGMTALVTGGTRGIGYAIVEELAELGAVVHTCSRNQTELNERLQEWEGKGFRVSGSVCDLTSRAQREKLMETVSSLFEGKLNILVNNAATVALKATTNYTAEDCSSILQTNFESPYHLCQLAHPLLKASGRGSIIFISSVAGLISLPHMSVYAATKGAINQVTKNLACEWAKDNIRTNAVAPWTVRTSVRPIMEPDNPMATSYPQLLARTPLHRIAEPDEVSPLVAFLCLPAASYITGQVICVDGGFSVNGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZVF5</accession>
    <name>A0AA38ZVF5_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_008344</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZVF5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009536">
        <property type="term" value="C:plastid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36773">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36773:SF1">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9696036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="98" mass="10882" checksum="22ACF5667672A54D" modified="2024-01-24" version="1">MAACVAASKEKCVKFAKDKCLGPFRDARIAVNGRDLTRKKKEEAVELIFWASMGGRRNSIGLENLGSWVLSKCKLGETNYRGSDLLDTQNVENSLCLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZZM1</accession>
    <name>A0AA38ZZM1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PTM/DIR17-like Tudor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_007224</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZZM1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd20404">
        <property type="entry name" value="Tudor_Agenet_AtEML-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047365">
        <property type="entry name" value="Tudor_AtPTM-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21743">
        <property type="entry name" value="PTM_DIR17_Tudor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="503"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="domain" description="PTM/DIR17-like Tudor" evidence="3">
        <location>
            <begin position="143"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="219"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="315"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="453"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="219"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="335"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="345"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="363"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9698014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="539" mass="59522" checksum="70A9E7A16795B30C" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A0C6</accession>
    <name>A0AA39A0C6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">MLO-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">MLO</name>
        <name evidence="10" type="ORF">PVL29_009444</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">May be involved in modulation of pathogen defense and leaf cell death.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="8">The C-terminus contains a calmodulin-binding domain, which binds calmodulin in a calcium-dependent fashion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the MLO family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A0C6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004326">
        <property type="entry name" value="Mlo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31942">
        <property type="entry name" value="MLO-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31942:SF52">
        <property type="entry name" value="MLO-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03094">
        <property type="entry name" value="Mlo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="6 8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 8" id="KW-0568">Pathogenesis-related protein</keyword>
    <keyword evidence="4 8" id="KW-0611">Plant defense</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="18"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="63"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="142"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="270"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="295"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="351"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="394"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU280816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9697618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="482" mass="55179" checksum="B70064454610F2E2" modified="2024-01-24" version="1">MAGGGAEEETTLEYTPTWVVAVVCTIIVAISLAVERLLHFLGKLLKKKNQKPLFQALQKIKEELMLLGFISLLLTVFQNLITKFCVPKHVVSHLLPCKLPEEKHATQSLSQWALGRHLLSSAPSSCSNEKVPFLSLEALHHLHIFIFVLAIVHVTFCVLTIIFGGAKIHQWKLWEDSVAKDNYDTEEVLKKKVTHVHDHAFIKDRFLGIGKNSAVMSWVHSFIKQFYASVTKSDYLTLRLGFIMTHCRQNRKFNFHKYMVRALEDDFKKVVGISWYLWIFVVVFLLLNVDGWHTYFWIAFIPFILLLAVGAKLEHVISQLAHEVAEKHAAIEGELVVKPSDDHFWFHRPRIVLFLIHIILFQNSFEMAFFFWILFTYGFDSCIMGKVQYIIPRLVIGVIIQVLCSYSTLPLYAIVTQMGTHFKKAIFDEHVQAGLVGWAQKARMKTTTGNPNRGTAIQLGRFVRSENAKEEITLTSAAEENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A1E4</accession>
    <name>A0AA39A1E4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Protein disulfide-isomerase</fullName>
            <ecNumber evidence="14">5.3.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_007294</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Acts as a protein-folding catalyst that interacts with nascent polypeptides to catalyze the formation, isomerization, and reduction or oxidation of disulfide bonds.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>Catalyzes the rearrangement of -S-S- bonds in proteins.</text>
            <dbReference type="EC" id="5.3.4.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the protein disulfide isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.4.1" evidence="14"/>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A1E4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003756">
        <property type="term" value="F:protein disulfide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02961">
        <property type="entry name" value="PDI_a_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02995">
        <property type="entry name" value="PDI_a_PDI_a'_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02982">
        <property type="entry name" value="PDI_b'_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02981">
        <property type="entry name" value="PDI_b_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000023">
        <property type="entry name" value="Protein disulfide-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000109">
        <property type="entry name" value="Protein disulfide-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000134">
        <property type="entry name" value="Protein disulfide-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000042">
        <property type="entry name" value="protein disulfide-isomerase A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005788">
        <property type="entry name" value="PDI_thioredoxin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005792">
        <property type="entry name" value="Prot_disulphide_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01130">
        <property type="entry name" value="ER_PDI_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01126">
        <property type="entry name" value="pdi_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18929">
        <property type="entry name" value="PROTEIN DISULFIDE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18929:SF246">
        <property type="entry name" value="PROTEIN DISULFIDE ISOMERASE-LIKE 1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13848">
        <property type="entry name" value="Thioredoxin_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00421">
        <property type="entry name" value="THIOREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 14" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10 12" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041485960" description="Protein disulfide-isomerase" evidence="14">
        <location>
            <begin position="26"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="16">
        <location>
            <begin position="68"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="16">
        <location>
            <begin position="421"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="26"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="548"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="29"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="46"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="59"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="557"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="12">
        <location>
            <begin position="124"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="12">
        <location>
            <begin position="463"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00054003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR605792-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9698129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="577" mass="64367" checksum="04F1AF81912CDAD5" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A1X9</accession>
    <name>A0AA39A1X9_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_008153</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9699426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A1X9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008586">
        <property type="entry name" value="DUF868_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31972">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31972:SF48">
        <property type="entry name" value="OS04G0407500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05910">
        <property type="entry name" value="DUF868"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9699426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="299" mass="33347" checksum="7ED0AAC24839E7F1" modified="2024-01-24" version="1">MRDIASCFGEYSVNVSDSSCSSYSNNACLSPSLIPSIQNAVTSVYKINLSTKKQLLIAVTWCKNQLGQGLSINLGDDPSAAFKLNTNSRLFRKKKGNKLLESDNLKIEVFWDLSTAKYNTGPEPVEGFYVMVVVDSEIGLLLGDMAEEAATRKLKSGTPVAKASLVSRREHCSGNTLYSTKAQFCDTGTAHDILIKCSGENEGLKHPVLSVSIDKKVVIRVKRLQWNFRGNQTIFVDGLLVDLMWDVHGWFFNPASGYAVFMFRTRSGMDSRLWLEEKLLQKDQDRLEFSLLIYACKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A287</accession>
    <name>A0AA39A287_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Transmembrane 9 superfamily member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_008212</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the nonaspanin (TM9SF) (TC 9.A.2) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9699511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A287"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072657">
        <property type="term" value="P:protein localization to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004240">
        <property type="entry name" value="EMP70"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766:SF119">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02990">
        <property type="entry name" value="EMP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="248"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="309"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="349"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="379"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="408"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="470"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="502"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="539"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="570"/>
            <end position="591"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9699511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="608" mass="69409" checksum="BB04E063E15BA8FD" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A7W3</accession>
    <name>A0AA39A7W3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_004383</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A7W3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12195">
        <property type="entry name" value="CIPK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14663">
        <property type="entry name" value="STKc_SnRK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000279">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000096">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.80:FF:000005">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.80">
        <property type="entry name" value="Kinase associated domain 1, KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018451">
        <property type="entry name" value="NAF/FISL_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004041">
        <property type="entry name" value="NAF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895">
        <property type="entry name" value="CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895:SF162">
        <property type="entry name" value="CBL-INTERACTING SERINE_THREONINE-PROTEIN KINASE 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03822">
        <property type="entry name" value="NAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50816">
        <property type="entry name" value="NAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="6 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="12"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="NAF" evidence="15">
        <location>
            <begin position="303"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9702618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="447" mass="50621" checksum="69E93E7D5945E9E2" modified="2024-01-24" version="1">MEEVRNMLFGKYEMGRLLGKGTFAKVYYAKHMVSGEGVAIKVINKDYVKKEGMMEQIKREISVMRLVRHPNIVELKEVMATKTKIFFVMEYVRGGELFAKVAKGKLKEDQSRKYFQQLISAIDFCHSRGVSHRDLKPENLLLDENEDLKVSDFGLSALPEQLRNDGLLHTQCGTPAYVAPEVLRKKGYDGAKADMWSCGVILFVLLAGFLPFQEENLMKMYRKVFKAEYQLPPWFSSEAKRLISKLLVADPQRRITIPAIMRVPWFRKGFSRPVAFSIEEPFPDKPEDSDILKLEAAKSTNSPSPAFFNAFEFISSMSSGFDLSSLFENQKKVGSMFTSKCSAAAIMAKIELVAKGLKFRVAKVKDFKLKLQGQSEGRKGRLSLTAEVFEVAPEVAIVELLKSAGDTLEYAKFCEEDVRPALKDIVWTWQGDNHNVNVSNNIVECEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A966</accession>
    <name>A0AA39A966_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_004830</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A966"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22162">
        <property type="entry name" value="F-box_AtSKIP3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025886">
        <property type="entry name" value="PP2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32278">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32278:SF130">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14299">
        <property type="entry name" value="PP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9703201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="277" mass="31442" checksum="5222B2E94C02E0EA" modified="2024-01-24" version="1">METESIIQATSSKFCAGVDFSALPEGCIAGILAWTSPRDACRMSTVSPEFLRAAESDALWETFLPADYREIIGRSPESSALLDFSSKKELFFRLCNSPLLIDGGKKSFWLDKESGKKFYMIAARELAIIWSETPRYWTWISLPQSRFTEVANLKSVCWFDIKGKMNTCMLSPGTNYAAYLIFQRNDIFYGFEDNPIESSIGVTGGESTKKVIYLDPEHDISPNPTRIDGWYEVELGEFFNEGRESVELEMSIMEVNRLNWKTGLLIEGIEIRPKFCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A9E1</accession>
    <name>A0AA39A9E1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Splicing factor 3B subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_004976</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SF3B4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A9E1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048026">
        <property type="term" value="P:positive regulation of mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12334">
        <property type="entry name" value="RRM1_SF3B4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12335">
        <property type="entry name" value="RRM2_SF3B4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000059">
        <property type="entry name" value="splicing factor 3B subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000121">
        <property type="entry name" value="Splicing factor 3b subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034158">
        <property type="entry name" value="SF3B4_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034159">
        <property type="entry name" value="SF3B4_RRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052084">
        <property type="entry name" value="SF3B4_spliceosome_assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48030">
        <property type="entry name" value="SPLICING FACTOR 3B SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48030:SF3">
        <property type="entry name" value="SPLICING FACTOR 3B SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="25"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="112"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="194"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="255"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="279"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="290"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="363"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00070533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9703425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="373" mass="40041" checksum="B2E46992CCD9628A" modified="2024-01-24" version="1">MTTRIAPGVGANLLGQHSAERNQDATAYVGNLDPQVTEELLWELFVQAGPVVNVYVPKDRVTNLHQGYGFVEFRSEEDADYAIKVLNMIKLYGKPIRVNKASQDKKSLDVGANLFVGNLDPDVDEKLLYDTFSAFGVIVTNPKIMRDPDTGNSRGFGFVSYDSFEASDAAIEAMNGQYLCNRQITVSYAYKKDTKGERHGTPAERVLAASNPSAPKSRPHTLFASGPPTLPNVPQANGNMGAPMPPRPFANGVVPPVPIPTIRPPPPQSGAFPPMQLAGPPSWQGQPQQPGQPGPPPMMPPPPLQQFRPPPPSMPPPPQQVMPGLSRPPPPPALMGGPPPVWRPPPPPQQLAGRPPTMLQMSMPPPPPPPAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A9J9</accession>
    <name>A0AA39A9J9_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_005034</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A9J9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.270">
        <property type="entry name" value="Methyltransferase, alpha-helical capping domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005299">
        <property type="entry name" value="MeTrfase_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042086">
        <property type="entry name" value="MeTrfase_capping"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31009">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE:CARBOXYL METHYLTRANSFERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03492">
        <property type="entry name" value="Methyltransf_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9703518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="365" mass="40331" checksum="1AA1EAB334B5A6C9" modified="2024-01-24" version="1">MLLPTREAKLLVKMLPMNGGNGKSSYGNNSLLQKRIILEVRPFLEDSIKDAFTSGIPNCVKLADLGCASGPNALSAISEIIHTIHGMSRGMNCKSPEFQVFLNDLPGNDFNNIFSLLPDFYEKLTKEDGTLGNCFITGVPGSFYSRIFPSRSLDFVHSSCSVHWLSQAPAGLEKNKGHIYIANGSPPTVIQAYTNQFQRDFSLFLGLRSEEIKPGGRMVITTIGRSMEDPSGGECCDLLELLAKSLSDMLAEGLIEEADLNSLNFAFYFPSEGEVRALVQEEGSFNLDKLVTFEASWDPFDESDKYRGAQNVANYMRSIAEPTLTSHFGGIIIGNLFGRYADHLAKHLLMEEGKYFFMVISLTNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A9Y4</accession>
    <name>A0AA39A9Y4_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_005142</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A9Y4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04458">
        <property type="entry name" value="CSP_CDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011129">
        <property type="entry name" value="CSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019844">
        <property type="entry name" value="CSD_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002059">
        <property type="entry name" value="CSP_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46565">
        <property type="entry name" value="COLD SHOCK DOMAIN PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46565:SF20">
        <property type="entry name" value="COLD SHOCK DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00313">
        <property type="entry name" value="CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00050">
        <property type="entry name" value="COLDSHOCK"/>
    </dbReference>
    <dbReference type="SMART" id="SM00357">
        <property type="entry name" value="CSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00352">
        <property type="entry name" value="CSD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51857">
        <property type="entry name" value="CSD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CSD" evidence="4">
        <location>
            <begin position="5"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="120"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="153"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="179"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="224"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="65"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="85"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9703725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="243" mass="23485" checksum="160316E848218537" modified="2024-01-24" version="1">MAQERSTGVVRWFSDQKGFGFITPNEGGEDLFVHQSSIKSDGFRSLGEGETVEFQIVLGEDGRTKAVDVTGPDGSSVQGSKRDSYGGGGGGGRGGRSGGGYGSGWRVGDRGGNGGGGAACYNCGGTGHLARDCVRRNNGGGGGGGGGGGGGGCYTCGQPGHLARDCSRPSGGGGGGGGCYNCGDYGHLARDCTQESGSAGRFGGGGGGRFGGGGGGGGGGGGGCYNCGKEGHFARECPNPNPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AAB1</accession>
    <name>A0AA39AAB1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_005205</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AAB1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000464">
        <property type="entry name" value="RNA-binding (RRM/RBD/RNP motifs) family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050441">
        <property type="entry name" value="RBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48034">
        <property type="entry name" value="TRANSFORMER-2 SEX-DETERMINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="59"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="135"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="22"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="153"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="197"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9703832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="229" mass="26602" checksum="5B0A7F7F7D4F8A3E" modified="2024-01-24" version="1">MADSPRKRYSKSPSPWREQSRSRSRSRSRSRSWSRPRHRSRSRSTSRSRGRIDTSNPGNTLYVTGLSTRVTERALEDHFSREGKVASCFLVMEPRTRISRGFAFVTMETVEDANRCIKHLNQSVLEGRYITVEKSRRKRARTPTPGHYLGLKNTRDSGYRGDRGRYRGGSGRDDYGYRRSPRPSPYRGGRDYSPRRSPPRGRSRRDRSRSIPYSPPYASPERGYGHRAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AAB5</accession>
    <name>A0AA39AAB5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phospholipase-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_005068</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AAB5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007942">
        <property type="entry name" value="PLipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35358">
        <property type="entry name" value="OS06G0711100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05278">
        <property type="entry name" value="PEARLI-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="440"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="21"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="35"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9703579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="505" mass="56027" checksum="F99859FE0BFC345D" modified="2024-01-24" version="1">MLDEETREELVEEDGTDTSMKRNRLSGRRARKRKEQGWGKIKEENDSQLLTNSFSQGPSSHAKIVPYSCASLMQAKALREAEIRIVDMLAKTVEGSTPASEDIQNSLKCTTSKPLPHIHGAEIHSTAAHSVPIQTLRDDFLLSVFSKPPMVSPVTPSRYTTRHAKMVARQRASLRHISSQEFEENHLSASPMEPLVAMPRDSSHAPSRYYRRHATTVLHKHANLSSVSTQGPEEKHPGGPASLLLEAAIGNPAPIGPVPFAATTNGREHIPLGEVTTASNIFENRPEESTHNQEQENVHLSQSLTLSLSEMAGLWDDGTSGDSGVGFLAVDSSSVTVGEYRVKEEFAPILRAVLLKHGDIAGNCSLQTMQSRSSFLEIVCAIVHKLQTTSLPYLTPVEINSMLAIVVDLELVKLEVGWLHQRLKEILDAMHLVKNITTLKEEQKKKYYLMEETKKELEALQEKVHLTEDKLAAMKAEAYKFSETISDAKTKVARLYQRPLLADLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ADL2</accession>
    <name>A0AA39ADL2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_003358</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ADL2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044715">
        <property type="entry name" value="WDR86-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44489">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44489:SF1">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <keyword evidence="3 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="24"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="128"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="161"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="284"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="24"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="128"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="64"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="71"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="88"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="100"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9705259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="411" mass="45493" checksum="E6ED143949EE8FEB" modified="2024-01-24" version="1">MDIKPSRLVGDKNERGRRHVNNMVCSYWLRGRCNRNPCRFLHQELPQNTHYQISNQFRKNCWQRNPDSDSKSASSLTSLDRSSGSTTPNRSSASNQSHRNNRERTLYSDRKGSSSGNGITHKVIGERVCKYWLHGNCVEADKCQYLHSWFKGHGVFKLAELNGHIMAISGIVLPSGSEKLYTASRDGYIRVWDCYTGQCDGVVNLGGEIGSLISAGPWVFAGIKDVVKAWNIESCADLSLDGPVGQIYAMVVNNDMLFARAQNGTIHAWKANKETNAFEPATTLEGHNGAVVSLTVGGGRLYSGSMDNTIRVWFATEAGKLEVTYTHNEEHGVLSLFGMNNSEGKLILLCSCNDNSVRLYELPSFTERAKIFAKEEVRAIQIGPGGLFFIGDGTGQVDVWKWQAEPTSLAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ADT1</accession>
    <name>A0AA39ADT1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_003649</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ADT1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.40">
        <property type="entry name" value="RutC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035959">
        <property type="entry name" value="RutC-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55298">
        <property type="entry name" value="YjgF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9705677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="95" mass="10164" checksum="86DD83BAF3872935" modified="2024-01-24" version="1">MLTWHKIPGHYSIGRTSVATTNLWRSSSSSLRSKPLACLSISTDASIKQAVQMDKASASLGPYSQAIKANDLVSASGVLGLIPETEKFILDNVED</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AEB6</accession>
    <name>A0AA39AEB6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">F-box protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_003012</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AEB6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010099">
        <property type="term" value="P:regulation of photomorphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037476">
        <property type="entry name" value="PCH1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36062">
        <property type="entry name" value="OS01G0687300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36062:SF1">
        <property type="entry name" value="OS01G0687300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="525"/>
            <end position="556"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9704759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="759" mass="85014" checksum="77223CE1FD586332" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AEI6</accession>
    <name>A0AA39AEI6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glycoside hydrolase family 5 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_001562</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AEI6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31263">
        <property type="entry name" value="CELLULASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_5G14560)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31263:SF0">
        <property type="entry name" value="CELLULASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_5G14560)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00150">
        <property type="entry name" value="Cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="Ricin B-like lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041398144" description="Glycoside hydrolase family 5 domain-containing protein" evidence="5">
        <location>
            <begin position="29"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="6">
        <location>
            <begin position="70"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9706051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="523" mass="59986" checksum="99270508FCA8D381" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AG69</accession>
    <name>A0AA39AG69_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_003646</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AG69"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9705674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="252" mass="27866" checksum="CF94BA7D1777D1BE" modified="2024-01-24" version="1">MYYPVLNGTLSLLRHSAEFDSSSVCYSPVLNRTPSHARYSAQFDSPPVSYSRVLNWTRSLPRYSAEFDSPSVAYSQSNSTPCLSHILRSSVGHDLFPGTQPNSVNSLSRILWCSTGKDLFLGTQSNSIPSLSRILRCSIGHDLFPGTQSNSIPVCLVFSHAQWDTISSSALSRIRFPICLVFSDSQLDKISCSVLSRIRFLVCLIFSDSQPDKISCSVLSRIRFPVCLIFSNSQPTLSLARYSAQFVSPSIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AGZ0</accession>
    <name>A0AA39AGZ0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Auxin-responsive protein SAUR36</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_002008</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ARG7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AGZ0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009733">
        <property type="term" value="P:response to auxin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003676">
        <property type="entry name" value="SAUR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31374">
        <property type="entry name" value="AUXIN-INDUCED PROTEIN-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31374:SF304">
        <property type="entry name" value="OS04G0537100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02519">
        <property type="entry name" value="Auxin_inducible"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9706820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="163" mass="18970" checksum="55AAB00DB7235B6B" modified="2024-01-24" version="1">MRKTRGFRLGRKLVTVFRWAFRRNQSPRVYKSLEHQSCRSRAMTKICNWGRRLQMKAKGLCFPKSGYVRVGHEPCETKPMEVPKGHLAVYVGDSGDDTHRVLVPVLYFNHPLFGELLRNAEKVYGFNHPGGITIPCPITEFEKVKTRIAAGEHFRRYRSTQRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AHK1</accession>
    <name>A0AA39AHK1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">UDP-glycosyltransferase 83A1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_002371</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AHK1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080043">
        <property type="term" value="F:quercetin 3-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080044">
        <property type="term" value="F:quercetin 7-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000061">
        <property type="entry name" value="UDP-glycosyltransferase 83A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000108">
        <property type="entry name" value="UDP-glycosyltransferase 83A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926">
        <property type="entry name" value="GLUCOSYL/GLUCURONOSYL TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926:SF1555">
        <property type="entry name" value="UDP-GLYCOSYLTRANSFERASE 83A1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9707344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="453" mass="50468" checksum="2B601046E3D7FE39" modified="2024-01-24" version="1">MGKPHILVVPLPAQGHVLPLMELSLCLAKQGLRVTFVNSEFIHERLVNALMERDNLGDQFRLVSIPDGLTDADRIIPGKLSEAIWGIMGEKLEELIGMIKRAGDDVSCVVADRGVGSALEVAAKMGIRRAAFCPTAAIITPLVFSIPKLINDGIIDNEGTPIKGQEIQFLPTNIPAINTKDFPWVWNGNLTMQKHLFKLIVRNNEAVKKADWLICNSAYDLEPSAFALAPEIIPVGPLLARNRLGNSAGSLWPEDSTCLKWLDQHPPCSVIYVAFGSMTIFNEKQFKELALGLELSNMPFLWVVRPNSIDCTKVAYPEGFQDRIAYRGKIVGWAPQQKVLSHPSVACFLSHCGWNSTIEGVSNGVSFLCWPYSVDQFLNERYISDVWKVGLGFNPDERGIITREEIKHKVEQLLGDENFRIRASNLKESAMNCVREGGSSYNNLQRFIQWLKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AHZ9</accession>
    <name>A0AA39AHZ9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Cytochrome P450 86B1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_000179</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9707991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AHZ9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11064">
        <property type="entry name" value="CYP86A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000044">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24296">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12 13" id="KW-0349">Heme</keyword>
    <keyword evidence="9 12 13" id="KW-0408">Iron</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 13" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="8 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="12">
        <location>
            <position position="480"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9707991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="540" mass="61726" checksum="8AFD41E39CA10941" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AI26</accession>
    <name>A0AA39AI26_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_002441</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AI26"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015495">
        <property type="entry name" value="Myb_TF_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47998">
        <property type="entry name" value="TRANSCRIPTION FACTOR MYB51-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47998:SF74">
        <property type="entry name" value="TRANSCRIPTION FACTOR TT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH myb-type" evidence="5">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="4">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9707420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="160" mass="18730" checksum="B038F01D80EE946B" modified="2024-01-24" version="1">MIRLHNLLGNKWSLITGRLPGRTANDVKNYWHPHRRDKTQTHSKTKVLKPQPYKLSKPSPRFELKTIAVIKPSSRSPHPNDDIALWESLLAGHAQMDQETDFLIFSSRGELISSLWVEETTTQTREPWMVWWNKSREVKVCGPRISTHVFPTQWCARFLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AJ98</accession>
    <name>A0AA39AJ98_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_002475</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AJ98"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039537">
        <property type="entry name" value="Retrotran_Ty1/copia-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057670">
        <property type="entry name" value="SH3_retrovirus"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648">
        <property type="entry name" value="TRANSPOSASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648:SF28">
        <property type="entry name" value="TRANSPOSON-ENCODED PROTEIN WITH RIBONUCLEASE H-LIKE AND RETROVIRUS ZINC FINGER-LIKE DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25597">
        <property type="entry name" value="SH3_retrovirus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="3">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9707458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="599" mass="67628" checksum="CD75C5DD72292F95" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AKQ2</accession>
    <name>A0AA39AKQ2_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_001042</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AKQ2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37178">
        <property type="entry name" value="PLANT/PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37178:SF1">
        <property type="entry name" value="PLANT_PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9709382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="283" mass="31673" checksum="523AFF3208D5CA7E" modified="2024-01-24" version="1">MDSLTSVSKPSIVFPPSNPLLPQRPRLVSLSLSLSSPASDARLRPRLSSFFALPCSPPHLSARRFLDVSCKSGAHDDADDLERALNLDGTIPGTSNEFVKRVSSRAYDMRRHLQQTFDSSSYDVLDANPWRETSKPVYVLTQKENQLCTMKTRRNRSEVERELGLLFSKGGKWRAKIGNQAKQSKTGTKTGTKFQMLVEDIREGVLVFEDENEAAKYCDLLQGGGQGCEGVAEIDASSVFDLCRKMRALAVLFRRGRTPPLPESLRLNLRARKRSLEDQEDLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D7H9</accession>
    <name>A0AA39D7H9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MOSC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_026284</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D7H9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030151">
        <property type="term" value="F:molybdenum ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005302">
        <property type="entry name" value="MoCF_Sase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14237:SF80">
        <property type="entry name" value="MOLYBDENUM COFACTOR SULFURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14237">
        <property type="entry name" value="MOLYBDOPTERIN COFACTOR SULFURASE MOSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03473">
        <property type="entry name" value="MOSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141673">
        <property type="entry name" value="MOSC N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51340">
        <property type="entry name" value="MOSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MOSC" evidence="1">
        <location>
            <begin position="566"/>
            <end position="733"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9672955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="735" mass="81590" checksum="F44ACAFC4577ECDB" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D7L1</accession>
    <name>A0AA39D7L1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_025694</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D7L1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041241388" evidence="1">
        <location>
            <begin position="21"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9672157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="305" mass="23682" checksum="6C7E24F30915C97D" modified="2024-01-24" version="1">MASKFLLLVLFGALVCTTSAVRKLLSQKGSFEDEKNFFHHHPGYGGGLGGGGGGGLGGGGGGGLGGGAGAGGGAGFGGGAGVGGGGGLGGGGGGGFGGGGGGGLGGGAGGGFGGGAGGGAGGGLGGGAGGGGGGGFGGGGGVGGGVGGGAGAGFGGGAGGGVGGGLLVSQKGSSEDEKMFFFGPGYDGGLGGGGGGGFGGGGGGGLDGGAGFGGGSGFGAGGGLGGGGGAGGGGGFGGGGGGGLGGGSGFGGGAGAGFGGGSGVGGGAGGGGGGGFGGGGGGGLGGGAGAGFGGGAGGGLGGGFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D9F9</accession>
    <name>A0AA39D9F9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_024162</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D9F9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000162">
        <property type="entry name" value="Adenine nucleotide alpha hydrolase-like domain kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000095">
        <property type="entry name" value="protein STRUBBELIG-RECEPTOR FAMILY 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47989:SF14">
        <property type="entry name" value="INACTIVE PROTEIN KINASE SELMODRAFT_444075"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47989">
        <property type="entry name" value="OS01G0750732 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="305"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="333"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9675100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="595" mass="67531" checksum="D281EED22CB59602" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D9X3</accession>
    <name>A0AA39D9X3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome b6-f complex subunit 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_023851</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D9X3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009512">
        <property type="term" value="C:cytochrome b6f complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00396">
        <property type="entry name" value="Cytb6_f_PetM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053333">
        <property type="entry name" value="Cytochrome_b6-f_sub7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012595">
        <property type="entry name" value="PetM_cyt_b6/f_cplx_su7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34951">
        <property type="entry name" value="B6F COMPLEX SUBUNIT, PUTATIVE, EXPRESSED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34951:SF1">
        <property type="entry name" value="B6F COMPLEX SUBUNIT, PUTATIVE, EXPRESSED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08041">
        <property type="entry name" value="PetM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103441">
        <property type="entry name" value="PetM subunit of the cytochrome b6f complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="90"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9674557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="122" mass="12387" checksum="B02F38D3025CECAA" modified="2024-01-24" version="1">MATASAILCPATVTARVRSSGSKTRKGEKKVVYVAGLNSFGGLKAHNSVVAMGVPVCTEQCFANVVSSLKAKGRGGGGALSSTCNAASEIFRIAAIMNALVLIGVAVGFVLLRIEASVEESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DB26</accession>
    <name>A0AA39DB26_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">HSF-type DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_020585</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the HSF family. Class A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DB26"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000057">
        <property type="entry name" value="Heat shock transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF427">
        <property type="entry name" value="HEAT SHOCK FACTOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00056">
        <property type="entry name" value="HSFDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00434">
        <property type="entry name" value="HSF_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0346">Stress response</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HSF-type DNA-binding" evidence="11">
        <location>
            <begin position="59"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="108"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="145"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="119"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9678443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="511" mass="56672" checksum="55846948E13EDAD5" modified="2024-01-24" version="1">MAASPAISSAASISNLPPPFLSKTYDMVDDRATDSVVSWSSGNNSFVVWNVPEFSRDLLPKFFKHNNFSSFVRQLNTYGFRKVDPDRWEFANEGFLRGQKHLLKSISRRKSTHVHTHNQQLQQQQQQQQQTQVQSSSGACVEVGKFGLEEEVERLKRDKNVLMQELVRLRQQQQTTDHQLQSVGQRVQDMEQRQQQMMSFLAKAVQSPGFLAQLVQQQNDSKRRITGVNKKRRLPKQEEEILSAKHATTAPDGRVVKYQPLMNEAAKAMLRQILKMNASPRREPSINPDAFLIDDVPSSNALDSLSSSSRVSGVTLSEVSPTSGQSYLPVESAYSSNHLSAGISETQSSPTVMTDSVKAAQFPEVNVPNSQKDTVLTDFTQMEGIIPESTVEIPNVGMVGTETGSPGYMDPMAGILDGVVPAETDFSLDSNVDSLLDGIPKLPGINDAFWEQFLATSPLTGDTDEIHSSMLEDNVVNEHELQPGVNGWNNTQHMDHLTERMGLLASKAKGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DB44</accession>
    <name>A0AA39DB44_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_022558</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DB44"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045182">
        <property type="entry name" value="JINGUBANG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22844">
        <property type="entry name" value="F-BOX AND WD40 DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22844:SF331">
        <property type="entry name" value="SIMILARITY TO GTP-BINDING REGULATORY PROTEIN AND WD-REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="66"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="187"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="230"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="275"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="320"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9677636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="409" mass="44844" checksum="AB30EF625A9BECCB" modified="2024-01-24" version="1">MGIMSAHQPSHSGDYSPSQFIHAQSVLISLSSQPSLPSVPSLTSQTQQQQLYLPSTTTTHHCITTFKGHTSYISSLALAGNFLFSGSSDKEIRLWEQNLNSELDHENISNNVVAVGKGAVKSIVVLGDKLFSGHQDHKIRVWKINDHEPHLQKCTRVATLPTLGDRAIKLLTPNNHVQIRRHKKCTWVHHVDTVSALALSKDNSLLYSVSWDRSLKIWRTTDFKCLESVGNAHDDAINALALSHDGYIYTGSADKTIKVWRKAPEDKKHSQVATLEKHNSGINALALSTDGSVLYSGASDRSIVVWEKDYSGNMVVVGALRGHSKSILCLAVVSDVVFSGSADNTIRIWRGIDKSYSCLAILEGHTGPVKCLTAATDGYNPSNTSTSYLVYSGSLDCDIKVWQIFIPLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DDJ3</accession>
    <name>A0AA39DDJ3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_020097</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9681023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DDJ3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901259">
        <property type="term" value="P:chloroplast rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21608">
        <property type="entry name" value="RRM2_NsCP33_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050502">
        <property type="entry name" value="Euk_RNA-bind_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048289">
        <property type="entry name" value="RRM2_NsCP33-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025">
        <property type="entry name" value="OS02G0815200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025:SF7">
        <property type="entry name" value="RNA-BINDING (RRM_RBD_RNP MOTIFS) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0934">Plastid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6 8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="85"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="210"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9681023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="291" mass="31376" checksum="8F52087C44C87234" modified="2024-01-24" version="1">MASASLPSSSASSSSILCKSTPTAPKCYVSSSLPSSIHFAHPKLSCSISISLSRTRFSTVVAVAAEDINTDDSSPQESKSSVRPCELYVCNLPRSCGISELLSMFKPHGTVQSIEVCRNAETGASRGSGYVTMSSMREAKAAIAALDGSPQHLSLSLSINESFTRLSPAVKDVGGREMRVRFSADMNFRRRNSEALNSGPLRNLIFESPYKLYVGNLAWAIKPEDLRNHFSQFGTVVSARVVHDRKAGKHRSYGFLSFSSAAECEAAMSLNGKEFRGRSLVVSAGMKRVES</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DE66</accession>
    <name>A0AA39DE66_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_019927</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DE66"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000431">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000513">
        <property type="entry name" value="Protein NSP-INTERACTING KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000371">
        <property type="entry name" value="Protein NSP-INTERACTING KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000562">
        <property type="entry name" value="Protein NSP-INTERACTING KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051824">
        <property type="entry name" value="LRR_Rcpt-Like_S/T_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48006:SF28">
        <property type="entry name" value="LEUCINE-RICH REPEAT PROTEIN KINASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48006">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN DDB_G0281931-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041375714" description="Protein kinase domain-containing protein" evidence="8">
        <location>
            <begin position="24"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="306"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="405"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="263"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9680756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="677" mass="73639" checksum="0968651195785783" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DG14</accession>
    <name>A0AA39DG14_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Alpha/beta hydrolase fold-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_018519</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDXG' lipolytic enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DG14"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041470337" description="Alpha/beta hydrolase fold-3 domain-containing protein" evidence="2">
        <location>
            <begin position="28"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="3">
        <location>
            <begin position="15"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9682614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="77" mass="8392" checksum="D0917782F4A72160" modified="2024-01-24" version="1">MIVSQWGQPFSVFHKLSLCVLLGVSCGANIADYVARRSVEAGKLLDPVKVVAQILMYPFFIGSVPTKSEIKLANLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DHI2</accession>
    <name>A0AA39DHI2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_016354</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9683815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DHI2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005162">
        <property type="entry name" value="Retrotrans_gag_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33223">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33223:SF8">
        <property type="entry name" value="OS04G0172440 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03732">
        <property type="entry name" value="Retrotrans_gag"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Retrotransposon gag" evidence="2">
        <location>
            <begin position="231"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="530"/>
            <end position="551"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9683815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="551" mass="61475" checksum="81279187E360B966" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DIM9</accession>
    <name>A0AA39DIM9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Retrovirus-related Pol polyprotein from transposon TNT 1-94</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_017428</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DIM9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039537">
        <property type="entry name" value="Retrotran_Ty1/copia-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057670">
        <property type="entry name" value="SH3_retrovirus"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648:SF22">
        <property type="entry name" value="REVERSE TRANSCRIPTASE TY1_COPIA-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648">
        <property type="entry name" value="TRANSPOSASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25597">
        <property type="entry name" value="SH3_retrovirus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Retroviral polymerase SH3-like" evidence="5">
        <location>
            <begin position="55"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase Ty1/copia-type" evidence="4">
        <location>
            <begin position="269"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="179"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="179"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9685389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="503" mass="56644" checksum="75F3ECD42832D1F0" modified="2024-01-24" version="1">MFQMKVPKQFWADAVSTTCFLINRMPTVVLKGDIPYKVIHPQKSLFPIELRIFGCTCYVRDTRPSVTKLDPKVLKCVFLVYSRLQKGYRCFSTDLNKYLVSTDVVFSKDTSFFSSPKSYVSEEEDEEWLVYQMVNSRPTVGQSSMVDSDASLAHLGTVVDVPTAPIKPPIVQVYSRRPVTTDTCPTPAPSSSDPPGDLDLPISLRKGKRQCKSTYSIANFVSCDHLSSSSSVFVASIDSILVPKTVTEALNYPGWKNAMLEEIRALEDNHTWKLVDLPPGKKVVGSKWVFAVKVNPNGFVARLKAKLVARGYAQTYRVDYFDTFSPIAKLNSWMIHQLDIKNAFLHGDLEEEVYMEQPPGFVAQREYEKVCCLKKALYGLKQSPCAWFGKFSKEIQAFGMNKSKKDHSVFYKKSIAGIILLVVYVDDIVIIGNDHAGISDLKAFMHSKFHTKDLGELKYFPGIEVSRSKKGMFLSQRKYVLDLLEETGKIEAKPCTTSMVLNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DLW5</accession>
    <name>A0AA39DLW5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">DNA ligase IV</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Polydeoxyribonucleotide synthase [ATP] 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PVL29_014815</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ATP-dependent DNA ligase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9689321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DLW5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032807">
        <property type="term" value="C:DNA ligase IV complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003910">
        <property type="term" value="F:DNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006297">
        <property type="term" value="P:nucleotide-excision repair, DNA gap filling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07903">
        <property type="entry name" value="Adenylation_DNA_ligase_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17722">
        <property type="entry name" value="BRCT_DNA_ligase_IV_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07968">
        <property type="entry name" value="OBF_DNA_ligase_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3260.10:FF:000005">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000173">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000044">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000072">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3260.10">
        <property type="entry name" value="DNA ligase, ATP-dependent, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.30">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044125">
        <property type="entry name" value="Adenylation_DNA_ligase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012309">
        <property type="entry name" value="DNA_ligase_ATP-dep_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012310">
        <property type="entry name" value="DNA_ligase_ATP-dep_cent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016059">
        <property type="entry name" value="DNA_ligase_ATP-dep_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012308">
        <property type="entry name" value="DNA_ligase_ATP-dep_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036599">
        <property type="entry name" value="DNA_ligase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029710">
        <property type="entry name" value="LIG4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45997">
        <property type="entry name" value="DNA LIGASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45997:SF1">
        <property type="entry name" value="DNA LIGASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16589">
        <property type="entry name" value="BRCT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04679">
        <property type="entry name" value="DNA_ligase_A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01068">
        <property type="entry name" value="DNA_ligase_A_M"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04675">
        <property type="entry name" value="DNA_ligase_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117018">
        <property type="entry name" value="ATP-dependent DNA ligase DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56091">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00697">
        <property type="entry name" value="DNA_LIGASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00333">
        <property type="entry name" value="DNA_LIGASE_A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50160">
        <property type="entry name" value="DNA_LIGASE_A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ATP-dependent DNA ligase family profile" evidence="17">
        <location>
            <begin position="305"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="18">
        <location>
            <begin position="627"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="18">
        <location>
            <begin position="815"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="580"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="914"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1102"/>
            <end position="1122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="986"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1005"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="1020"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1032"/>
            <end position="1044"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00031942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ9689321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1138" mass="128930" checksum="183BD5B679BF09AC" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DMQ6</accession>
    <name>A0AA39DMQ6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FLZ-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_014711</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FLZ family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9689185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DMQ6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044604">
        <property type="entry name" value="FLZ12/13/14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007650">
        <property type="entry name" value="Zf-FLZ_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47208">
        <property type="entry name" value="OS02G0174800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47208:SF1">
        <property type="entry name" value="OS02G0174800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04570">
        <property type="entry name" value="zf-FLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51795">
        <property type="entry name" value="ZF_FLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FLZ-type" evidence="5">
        <location>
            <begin position="179"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="FLZ-type" evidence="4">
        <location>
            <begin position="179"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9689185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="252" mass="27330" checksum="CE336598D7CD84B6" modified="2024-01-24" version="1">MESFSGKGRLALNLSIFTNSFEPKSQVWSLDSKVSSTTKSPRNFEGGVVGLGIVAAMNDLDNTHDSLSSKPCKASILAATSPRSNPIPIANRAKRTPKVEKCRPSSEMMEMEMEMSESYTCVISHCGNNLIKKRVYLDDKLNGVVDNITTAVPISSTDAAPAAAYWVASEAAPPFKTADFLSSCYLCQKKLHGLDIFMYRGEKAFCSAECRSNQMVSDEYKEKCGSEAMKSFDYSVSPCSGPMLFFAGVAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DN03</accession>
    <name>A0AA39DN03_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DYW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_013937</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. PCMP-H subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9687952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DN03"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000333">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000690">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032867">
        <property type="entry name" value="DYW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF436">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN ELI1, CHLOROPLASTIC-LIKE ISOFORM X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14432">
        <property type="entry name" value="DYW_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="185"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="247"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="348"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="DYW" evidence="4">
        <location>
            <begin position="563"/>
            <end position="655"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9687952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="655" mass="73793" checksum="225C46BF251BB820" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DN05</accession>
    <name>A0AA39DN05_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Glutamate receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_012980</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Glutamate-gated receptor that probably acts as non-selective cation channel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DN05"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015276">
        <property type="term" value="F:ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13686">
        <property type="entry name" value="GluR_Plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19990">
        <property type="entry name" value="PBP1_GABAb_receptor_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000172">
        <property type="entry name" value="Glutamate receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000054">
        <property type="entry name" value="Glutamate receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000188">
        <property type="entry name" value="Glutamate receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044440">
        <property type="entry name" value="GABAb_receptor_plant_PBP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019594">
        <property type="entry name" value="Glu/Gly-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015683">
        <property type="entry name" value="Ionotropic_Glu_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001320">
        <property type="entry name" value="Iontro_rcpt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017103">
        <property type="entry name" value="Iontropic_Glu_rcpt_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18966">
        <property type="entry name" value="IONOTROPIC GLUTAMATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00060">
        <property type="entry name" value="Lig_chan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10613">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037090">
        <property type="entry name" value="Iontro_Glu-like_rcpt_pln"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00079">
        <property type="entry name" value="PBPe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 13" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 13" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="8 13 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 13" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="577"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="638"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="813"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor C-terminal" evidence="16">
        <location>
            <begin position="453"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="871"/>
            <end position="938"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR037090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9690596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="938" mass="105337" checksum="9D307EB609A2988F" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DQ91</accession>
    <name>A0AA39DQ91_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_011980</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9693068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DQ91"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9693068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="90" mass="9707" checksum="270FBF1176041B7E" modified="2024-01-24" version="1">MIFPQDVQSEFTVKDLLSGNAAIHANRSPFSDVVKITGEGSLSPSKTSQQPMTNFLGDANQVKLKLAAEGPEPQACLNPIYLILLILPKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DQY2</accession>
    <name>A0AA39DQY2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">B-like cyclin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_011502</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclin family. Cyclin AB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DQY2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044772">
        <property type="term" value="P:mitotic cell cycle phase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20506">
        <property type="entry name" value="CYCLIN_AtCycA-like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20562">
        <property type="entry name" value="CYCLIN_AtCycA_like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000013">
        <property type="entry name" value="Cyclin A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000167">
        <property type="entry name" value="Mitotic cyclin 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046965">
        <property type="entry name" value="Cyclin_A/B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004367">
        <property type="entry name" value="Cyclin_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02984">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001771">
        <property type="entry name" value="Cyclin_A_B_D_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01332">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3 5" id="KW-0195">Cyclin</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="7">
        <location>
            <begin position="267"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin C-terminal" evidence="8">
        <location>
            <begin position="360"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="7">
        <location>
            <begin position="364"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="25"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="187"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="38"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9692470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="490" mass="55121" checksum="0CAF10D55FD57289" modified="2024-01-24" version="1">MEKENWATANAEELTTRVTRARAAAYRASKQMLPKNASMPQEQKRTSQSNSKRPALEEKNNAASATTAPPCKKRAVLKDVRNICCENLYMNCINATKIQKGNSKQARKGLVMLPKVAPSITVEISLLPADPKATVAQEVEKQEIEPEVIRLTIDGNLPSQLTSGTTLRKCGIADNQLEKHCSEIASQLHSSSNEAEKGSFEGKLTTSSNPDVKDIDSDDKDPQLCSLYAPEIYNNLHVAELNRRPCSNFMETVQRDITQSMRGILVDWLVEVSEEYKLVPDTLYLTVHLIDWFLSQNYIERQKLQLLGITCMLIASKYEEICAPRVEEFCCITDNTYSRGEVVKMESQVLNYFGFKIFAPTAKTFLRRFLRAAQASYKNPSLELEYLGNYLAELTLIDYGCLKYLPSIIAASAVFLARWTLDQSGHPWNPTLEHYTRYKASDLKTAVFALQDLQLNTSGCPLNAIRGKYRQNKFKSVASLSSPKLLQTLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DRD3</accession>
    <name>A0AA39DRD3_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_011827</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DRD3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9692905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="45" mass="4991" checksum="826642D3EFD5BBA0" modified="2024-01-24" version="1">MADALLSIVLDRLASLIQQQIHWEVSLVVGVETENQSLTDTLQIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DRH3</accession>
    <name>A0AA39DRH3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutathione S-transferase</fullName>
            <ecNumber evidence="1">2.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_011939</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Is involved in the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GST superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="1"/>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9693025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DRH3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045073">
        <property type="entry name" value="Omega/Tau-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11260">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE, GST, SUPERFAMILY, GST DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11260:SF764">
        <property type="entry name" value="GLUTATHIONE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="2">
        <location>
            <begin position="3"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU369102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9693025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="61" mass="7214" checksum="62EA7AC6C2AD5C31" modified="2024-01-24" version="1">MADEIILLDFWPSMFGLRVRVALAEKGLKYEYRQEDLRNKSPLLLEMNPVRKKIPVLVLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DTZ5</accession>
    <name>A0AA39DTZ5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Glycosyltransferase 2-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_009331</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the glycosyltransferase 2 family. Plant cellulose synthase-like C subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DTZ5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099402">
        <property type="term" value="P:plant organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048868">
        <property type="term" value="P:pollen tube development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000007">
        <property type="entry name" value="probable xyloglucan glycosyltransferase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32044">
        <property type="entry name" value="GLUCOMANNAN 4-BETA-MANNOSYLTRANSFERASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32044:SF67">
        <property type="entry name" value="XYLOGLUCAN GLYCOSYLTRANSFERASE 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13632">
        <property type="entry name" value="Glyco_trans_2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="111"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="174"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="495"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="638"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="11">
        <location>
            <begin position="319"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF13632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9697466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="688" mass="79128" checksum="89F9D353BAE79081" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DUB6</accession>
    <name>A0AA39DUB6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CASP-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_008551</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3 8">Homodimer and heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Casparian strip membrane proteins (CASP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DUB6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006459">
        <property type="entry name" value="CASP/CASPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006702">
        <property type="entry name" value="CASP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044173">
        <property type="entry name" value="CASPL"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01569">
        <property type="entry name" value="A_tha_TIGR01569"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36488">
        <property type="entry name" value="CASP-LIKE PROTEIN 1U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36488:SF8">
        <property type="entry name" value="CASP-LIKE PROTEIN 1U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04535">
        <property type="entry name" value="CASP_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="85"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="119"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="170"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="Casparian strip membrane protein" evidence="9">
        <location>
            <begin position="30"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9696384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="202" mass="21533" checksum="BF96CC800D48934D" modified="2024-01-24" version="1">MESQYRPNVDGVHNGVESHVKVVEKPRSVGSSSEFVLRILGLLLTLIAAVVAGVNKQTKIIPLTLIKTLPSLHVPVTAKWSDMSAFVYFVVSNAIACSYAAISLVLVTMLGRRGKGGRVLAVIVLDLHMVGLLFSANGAAAAVGVLGQYGNSHVEWKKVCNVFDSFCHHLVASLALSFLGSLSFLGLVLLAILNLHKKSSTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DWV5</accession>
    <name>A0AA39DWV5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_006229</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DWV5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001751">
        <property type="entry name" value="S100/CaBP7/8-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043145">
        <property type="entry name" value="Znf_ZZ_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00303">
        <property type="entry name" value="S100_CABP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="25"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="59"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9700811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="173" mass="19709" checksum="83C518FAF19FDFDC" modified="2024-01-24" version="1">MEEIREAALAYYQNGSEYQKCSANDFFNSLDQDRNGKIDVHEFINILSEMGYGLVNNSRFFQELDRDGNGSLDFSEFLTLFYIIKCPRPFCDGCGILLKGLFFTCVQCYESSNNNTFDLCSACYRGKRFSHQHAAFLDNYTLLARKRPITLGGEEQPNTSQGVPAWRKGSLNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DXL4</accession>
    <name>A0AA39DXL4_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_008016</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9699205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DXL4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36045">
        <property type="entry name" value="OS04G0558500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36045:SF2">
        <property type="entry name" value="OS04G0558500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9699205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="146" mass="16494" checksum="B54B4107CC2E8A41" modified="2024-01-24" version="1">MALKEPQFRSSGDPMEGSDAEELEELEADVKEMAQKVRHYRTTLPDHLKATFTSIISSQRPPLLEFVSGSEPEASGEPNLRQSRVLLAQGDHETSEKIQLLKQKISSNVSTIPVLLKRLNECMSMVDKLDLYNGIMHPAFKRRRTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DZR9</accession>
    <name>A0AA39DZR9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Glutamate receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PVL29_004532</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Glutamate-gated receptor that probably acts as a non-selective cation channel. May be involved in light-signal transduction and calcium homeostasis via the regulation of calcium influx into cells.</text>
    </comment>
    <comment type="function">
        <text evidence="15">Glutamate-gated receptor that probably acts as non-selective cation channel.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">May form heteromers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DZR9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015276">
        <property type="term" value="F:ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13686">
        <property type="entry name" value="GluR_Plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19990">
        <property type="entry name" value="PBP1_GABAb_receptor_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000037">
        <property type="entry name" value="Glutamate receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000103">
        <property type="entry name" value="Glutamate receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000169">
        <property type="entry name" value="Glutamate receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000758">
        <property type="entry name" value="Glutamate receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000195">
        <property type="entry name" value="Glutamate receptor 2.7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044440">
        <property type="entry name" value="GABAb_receptor_plant_PBP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019594">
        <property type="entry name" value="Glu/Gly-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015683">
        <property type="entry name" value="Ionotropic_Glu_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001320">
        <property type="entry name" value="Iontro_rcpt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017103">
        <property type="entry name" value="Iontropic_Glu_rcpt_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34836">
        <property type="entry name" value="OS06G0188250 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34836:SF1">
        <property type="entry name" value="OS09G0428600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00060">
        <property type="entry name" value="Lig_chan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10613">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037090">
        <property type="entry name" value="Iontro_Glu-like_rcpt_pln"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00079">
        <property type="entry name" value="PBPe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13 15" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 15" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="9 15 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 15" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 15" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="555"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="619"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="800"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor C-terminal" evidence="19">
        <location>
            <begin position="429"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="905"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="727"/>
            <end position="783"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR037090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR037090-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAJ9702833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="926" mass="103261" checksum="DF7891D5CD93C4C1" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DZV4</accession>
    <name>A0AA39DZV4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Retrotransposon Copia-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_005697</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9699982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DZV4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33511">
        <property type="entry name" value="OS06G0632400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9699982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="171" mass="19398" checksum="162E035F686C1FC7" modified="2024-01-24" version="1">MAGNNRQKKSSFFSIFSIFKACCSPGWDDTWDEAGNYGRRVCPSDEDSHGWVAEPGIDRKASDFIAKFYATRASSMAFEDFSSSTLARSHQNGINSSPNTTWMMSSLNQAFTIRLDRSNYLLWRTQMLNIVIANGLEEMIDSSRPCPPKFLENSESTNPKFQIWFSACEKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DZZ3</accession>
    <name>A0AA39DZZ3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PVL29_005772</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DZZ3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031349">
        <property type="term" value="P:positive regulation of defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045088">
        <property type="term" value="P:regulation of innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000032">
        <property type="entry name" value="Serine/threonine-protein kinase PBS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000248">
        <property type="entry name" value="Serine/threonine-protein kinase PBS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47985">
        <property type="entry name" value="OS07G0668900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47985:SF4">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE PBL27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0564">Palmitate</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-0611">Plant defense</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="87"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="373"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="16"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="373"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="419"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="451"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="471"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAJ9700102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="528" mass="57857" checksum="073EDE1B1062D5D3" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E129</accession>
    <name>A0AA39E129_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Folate-biopterin transporter 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_004476</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Folate-biopterin transporter (TC 2.A.71) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E129"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd17484">
        <property type="entry name" value="MFS_FBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039309">
        <property type="entry name" value="BT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004324">
        <property type="entry name" value="FBT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00788">
        <property type="entry name" value="fbt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31585">
        <property type="entry name" value="FOLATE-BIOPTERIN TRANSPORTER 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31585:SF44">
        <property type="entry name" value="FOLATE-BIOPTERIN TRANSPORTER 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03092">
        <property type="entry name" value="BT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="162"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="204"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="231"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="291"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="324"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="355"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="15"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9702760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="518" mass="57413" checksum="B68F68CCB14B38EA" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E2T2</accession>
    <name>A0AA39E2T2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">40S ribosomal protein S16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_003237</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E2T2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000007">
        <property type="entry name" value="40S ribosomal protein S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000754">
        <property type="entry name" value="Ribosomal_uS9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020574">
        <property type="entry name" value="Ribosomal_uS9_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001749">
        <property type="entry name" value="PRK00474.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569:SF16">
        <property type="entry name" value="RIBOSOMAL PROTEIN S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569">
        <property type="entry name" value="RIBOSOMAL PROTEIN S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00380">
        <property type="entry name" value="Ribosomal_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00360">
        <property type="entry name" value="RIBOSOMAL_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9705084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="144" mass="16399" checksum="695CA2CAD9104863" modified="2024-01-24" version="1">MASPVESVQCFGRKKTAVAVTYCKRGRGLIKINGCPIELVEPEILRYKAVEPILLLGRHRFAGVDMRIRVKGGGHTSQIYAIRQSIAKALVAFYQKYVDEQSKKEIKDILVRYDRTLLVADPRRCEPKKFGGRGARARFQKSYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E5A7</accession>
    <name>A0AA39E5A7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_001466</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9710006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E5A7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044822">
        <property type="entry name" value="Myb_DNA-bind_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47211">
        <property type="entry name" value="TRIHELIX TRANSCRIPTION FACTOR ASR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47211:SF2">
        <property type="entry name" value="TRIHELIX TRANSCRIPTION FACTOR ASR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13837">
        <property type="entry name" value="Myb_DNA-bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="39"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="202"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="19"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9710006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="341" mass="37312" checksum="6136EF9D5491D99B" modified="2024-01-24" version="1">MELERLSLAPAPVDDDADAVPNGVNAAGDDGSRAPRLPRWTRQEILVLIQGKKVAESRVRRGRTGGLAFGSAAQIEPKWASVSSYCKRHGVNRGPVQCRKRWSNLAGDYKKIKEWESQTRDESESFWVMRNDVRREKRLPGFFDREVYDMLDGVGAAPPGPSGLALGLAPAPEGEGMVAPEEEAEAVFDSGRSAAAEDGLFSDFEQSGGSPEKEPQAKEAPATVAAPVPISEKQYQPFPREGSSQGPASKRHPASNPEMASTSQEGRKRKRFTADGDEETTRLQDQLIEVLERNGRMLSAQLEAQNTNFQLDREQRKDQADCLVAVLSKLADALGRIADKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E625</accession>
    <name>A0AA39E625_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_001245</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E625"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11072">
        <property type="entry name" value="CYP71-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000011">
        <property type="entry name" value="Cytochrome P450 83B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47955">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 71 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 5" id="KW-0349">Heme</keyword>
    <keyword evidence="3 4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="2 4 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="236"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="4">
        <location>
            <position position="438"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9709673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="505" mass="57166" checksum="2D1E1BC0A2EDC7D3" modified="2024-01-24" version="1">MDDYTPQTICLLAVLFITLLLLSRRKWLQSPALRTPPSPPALPIIGHLHLLTDPPHHSLADLALKLGPIIQLRLGQVATVVVSSARLADLVLKTHDHIFANRPPLTAAQHLSFGCTDVTFSHGPYWRQARKICVTELFSPKRVTSFQLIRNEETHHLITSLSALSGSETDLSQLFFTLANNVLCRVAFGKRFMDDSEGEKKHIVDVLTETQALFGGFCIGDFFPDWKWLNTITGFNRRLRKNLEDLIAVYNEIIEEHVNEEKEREDFVGVLLRVQKRKDLEVAITDDNMKAIVLDMFVAGTDSTASALEWTMAELARKPHMMRKAQQEVRNIASGEGKVEESHLPQLHYMKAAIKETMRLHPPVPLLVPRQSMENCILDGYEIPAKTRLLVNAYAIGRDPQSWENPLDFNPERFVDGDVDVRGQDTRFIPFGGGRRGCPGYSFGLATVEIALARLLYHFDWELPHGVEADDIDLSEIFGLTTRKKSALILVPRYNRDHEIKENDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E7W1</accession>
    <name>A0AA39E7W1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">1-phosphatidylinositol 4-kinase</fullName>
            <ecNumber evidence="2">2.7.1.67</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_001094</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PI3/PI4-kinase family. Type II PI4K subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.67" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E7W1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004430">
        <property type="term" value="F:1-phosphatidylinositol 4-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044571">
        <property type="entry name" value="P4KG1-8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45800:SF11">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3-KINASE-RELATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45800">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-KINASE GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="8">
        <location>
            <begin position="161"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="514"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="515"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9709449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="640" mass="71456" checksum="F94DABABDE4A63A9" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YGZ9</accession>
    <name>A0AA38YGZ9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DYW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_026678</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. PCMP-H subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YGZ9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001103">
        <property type="entry name" value="Glycerol-3-phosphate dehydrogenase [NAD(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000351">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001767">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At5g15340, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000285">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein, chloroplastic"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032867">
        <property type="entry name" value="DYW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046849">
        <property type="entry name" value="E2_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF342">
        <property type="entry name" value="TETRATRICOPEPTIDE-LIKE HELICAL DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14432">
        <property type="entry name" value="DYW_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20430">
        <property type="entry name" value="Eplus_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="121"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="223"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="324"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="425"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="DYW" evidence="4">
        <location>
            <begin position="639"/>
            <end position="732"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9670274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="732" mass="81954" checksum="E0D31D45964456A8" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YIK7</accession>
    <name>A0AA38YIK7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_027212</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9671112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YIK7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF78">
        <property type="entry name" value="OS12G0587133 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="331"/>
            <end position="599"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9671112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1033" mass="119037" checksum="9777F4F9AE7EC5AA" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YM07</accession>
    <name>A0AA38YM07_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Post-GPI attachment to proteins factor 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_026274</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in the lipid remodeling steps of GPI-anchor maturation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Golgi apparatus membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the PGAP3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YM07"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007217">
        <property type="entry name" value="Per1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13148">
        <property type="entry name" value="PER1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13148:SF0">
        <property type="entry name" value="POST-GPI ATTACHMENT TO PROTEINS FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04080">
        <property type="entry name" value="Per1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="2 7" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041489731" description="Post-GPI attachment to proteins factor 3" evidence="7">
        <location>
            <begin position="23"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="157"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="193"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="223"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="249"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="278"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="308"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9672945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="342" mass="39792" checksum="1CEA06C57FBEE63E" modified="2024-01-24" version="1">MVDRYWIAFFAVFVYLVRVFEASVGDADPLYRACVEQCEKTGCVGERCFPHCKFPSDGAVVDSPWYLQEPLYLRWKQWDCQSDCRYYCMLDREKEREALGNGPVKYHGKWPFKRVYGIQEPASVALSALNLAMQFHGWLSFFILLNYKLPLKPNKKAYYEYTCLWHIYGLLSMNSWFWSAVFHSRDVDLTEKLDYSSAVALLGFSLILAILRSFNVRVEAARVMVSAPLLAFVTTHILYLNFYKFDYGWNMKVCVVMGVAQLLIWAIWAGVTRHPSRWKLWTVVVGGGLAMLLEIYDFPPYEGFVDAHALWHATTIPLSYIWWSFIKDDAEFQTANLLKKVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YRE2</accession>
    <name>A0AA38YRE2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_024278</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily. AB hydrolase 4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YRE2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034338">
        <property type="term" value="F:short-chain carboxylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000071">
        <property type="entry name" value="Embryogenesis-associated protein EMB8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050960">
        <property type="entry name" value="AB_hydrolase_4_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10794">
        <property type="entry name" value="ABHYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10794:SF63">
        <property type="entry name" value="ALPHA_BETA HYDROLASE 1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="457"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9675256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="497" mass="55185" checksum="5B669D641D280EB9" modified="2024-01-24" version="1">MRSIQHKPHSLPTSFFSGLSLPYPFPIISWLPSSDVFSGLRGSPVSLTFNSHSQIYEGVVSKCRILHGRYLATPWLSNPHFQTAFLNFFGRPPVVNYRSDLSLDWINDTTPIVVVIHGLSSDSTSASDCFYNAGWTEDIREVIAHLHHEYPVAPLFAVGTSIGANILVKYLGEDRENIPVAGAVAICNPWDLLIGDRFISRRLLQKFYDMALTSGLQGYAQLHQPRYSRLADWEGIKKSRSIRDFDTYATCLVGKFETADTYYRRCSSASFVGNVAVPLLCVSALDDPVCTREAIPWDECRANKNVILATPQHGGHLAFFEGIAATSLWWVRAVDEFLSVLHSSPFMHKQKKILNSSSPSSLESAIDQGPYVNFAEDGMVAAMGNEQTSTVVGPLSEGQNIHDKSSDNEIASYSEQDERMTTENSALAPNIAQTSKLAAKPSSIRKCLEKLSQHNRISMWLMAYIAITTSWPLVGPALQFVFKNKFRSVLPAAFHGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YRH9</accession>
    <name>A0AA38YRH9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF4216 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_024238</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YRH9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025312">
        <property type="entry name" value="DUF4216"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48258">
        <property type="entry name" value="DUF4218 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48258:SF3">
        <property type="entry name" value="FK506-BINDING PROTEIN 4-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13952">
        <property type="entry name" value="DUF4216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4216" evidence="1">
        <location>
            <begin position="2"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9675208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="126" mass="14413" checksum="673E8C4BA9AA387B" modified="2024-01-24" version="1">MFRIPVFKCDWVDNKNGLKVDDLGFTLVDFSKIAHKSDPFILASQTKQVFYVQDQLDPRWSVVLSTPQKDFLDMEGGEDFVDNSIEHHPFIGALPQIEAFDVMDDSYATCMRGNCEGIWIENKSSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YSI3</accession>
    <name>A0AA38YSI3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cyclin-dependent protein kinase inhibitor SMR3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_024703</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YSI3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004860">
        <property type="term" value="F:protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032875">
        <property type="term" value="P:regulation of DNA endoreduplication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040389">
        <property type="entry name" value="SMR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33142">
        <property type="entry name" value="CYCLIN-DEPENDENT PROTEIN KINASE INHIBITOR SMR13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33142:SF114">
        <property type="entry name" value="CYCLIN-DEPENDENT PROTEIN KINASE INHIBITOR SMR14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0649">Protein kinase inhibitor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="31"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="69"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9675870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="143" mass="16325" checksum="AEC20453D451C123" modified="2024-01-24" version="1">MRKMSHFQDQPSTDHHLQEQEQEQEAQGNIHDPELIQEKGGDNHVDDTVPSQEPGRPSLGELSAVEENEGFRTPKSMEHKIPAITECPPAPRKPTATRAIPMKRRISKVPRRLQFEEFEVLIPPKILASLLSRLRKPSRDGRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YTB4</accession>
    <name>A0AA38YTB4_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_024867</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9676079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YTB4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33780">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33780:SF10">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9676079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="110" mass="12449" checksum="BF4FEA716BC4724E" modified="2024-01-24" version="1">MRGASGSGSRWRSLQFFHIIFPTISLLLISGLADDPSNSKDVKKAERHDTHGNMIGIRIVIICLGLVAGAAFSVFLFKIWQKKKREEQHARLLKLFEDDDELEVELGIRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YTB8</accession>
    <name>A0AA38YTB8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">LITAF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_025029</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9676305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YTB8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9676305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="69" mass="7705" checksum="3584D20C71EF9BB6" modified="2024-01-24" version="1">MGIPYPAVYHPNQNPYQAGGVPIQQTIFQQTPAPFNCVYCGSSGYNNGPRDTVWDFSCCCFVIEIDNRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YTS9</accession>
    <name>A0AA38YTS9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Auxin response factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_025089</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Auxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs).</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimers and heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ARF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9676397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YTS9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10017">
        <property type="entry name" value="B3_DNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.1040:FF:000001">
        <property type="entry name" value="Auxin response factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.330.10:FF:000001">
        <property type="entry name" value="Auxin response factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000047">
        <property type="entry name" value="Auxin response factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.330.10">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010525">
        <property type="entry name" value="ARF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044835">
        <property type="entry name" value="ARF_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033389">
        <property type="entry name" value="AUX/IAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003340">
        <property type="entry name" value="B3_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015300">
        <property type="entry name" value="DNA-bd_pseudobarrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31384:SF96">
        <property type="entry name" value="AUXIN RESPONSE FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31384">
        <property type="entry name" value="AUXIN RESPONSE FACTOR 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06507">
        <property type="entry name" value="ARF_AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02309">
        <property type="entry name" value="AUX_IAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02362">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01019">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101936">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50863">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0927">Auxin signaling pathway</keyword>
    <keyword evidence="4 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="TF-B3" evidence="11">
        <location>
            <begin position="157"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="PB1" evidence="12">
        <location>
            <begin position="551"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="392"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="514"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="653"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="519"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="653"/>
            <end position="668"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9676397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="675" mass="75366" checksum="A35D5F876F77F90B" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YUL7</accession>
    <name>A0AA38YUL7_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_022032</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9676827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YUL7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9676827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="95" mass="11019" checksum="52ADE89FE8873ED7" modified="2024-01-24" version="1">MFAVTDLNKTSHIQGHLQKTNQKDDGWMNLQKGLELYRIIFMTKTMGSIPSMPLQFNQTASSMRKLKLTLKGFSKKKPYYLSSRRGWLQPPNVKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YUM6</accession>
    <name>A0AA38YUM6_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_022143</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YUM6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000011">
        <property type="entry name" value="Myb transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000107">
        <property type="entry name" value="MYB transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044676">
        <property type="entry name" value="EOBI/EOBII-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45675">
        <property type="entry name" value="MYB TRANSCRIPTION FACTOR-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45675:SF117">
        <property type="entry name" value="MYB-RELATED PROTEIN MYBAS2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="19"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="19"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="72"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="72"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="175"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9677001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="332" mass="37564" checksum="D140F1DB954D7B2F" modified="2024-01-24" version="1">MRIAMSTLSKSASSSSEEDAELRRGPWTLEEDTLLIHYIASHGEGRWNLLAKCSGLRRTGKSCRLRWLNYLKPDVKRGNLTPQEQLLILELHSKWGNRWSKIAQHLPGRTDNEIKNYWRTRVQKQARHLKIDSNSRAFQDVIRCVWMPRLLQKIEGSSSTSSTTSQVSIIPQSLNHADSQQHSAPPPPPVGAQTPVQGPLSTNETINNLEHNEKNSSSENCTSPSICSSESMNISQISQIPDYPTSPFQVMSNSDDYSTLLKGYYNVDNSTYEMETFNLASMSAPQGDFENPVGDCPMVESNWVDSDLADSLWNMDELWQFWKLQEGGPRLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YVC3</accession>
    <name>A0AA38YVC3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sulfotransferase</fullName>
            <ecNumber evidence="3">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_022299</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YVC3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11783">
        <property type="entry name" value="SULFOTRANSFERASE SULT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="5">
        <location>
            <begin position="84"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="19"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9677222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="354" mass="40933" checksum="54D6927162D3898B" modified="2024-01-24" version="1">MATIFSAEKSLTSQTSDEAVEHEQEQEADEGRSSESYTEIISTLPREKGWTTEYMYQYQGFWYLSTTGVEGVMRVQKHFKARNEDILLATLPKSGTTWFKPLIFAIMNRGRCDFATHPVLTTSPHDLVPPLENYFHQNIAYPNLDSLSSPQLLFHTHISFTSLPQSVINSQGRIVYVCRNPKDVFVSTFYFLNRMADKNVEPLSVEEAFEQFCKGVSFYGPFWDHVLGYWKASLEWPERVLFMKYEDMKKDSPFHLKRLAEFMGCPFSSEEERQGVVHEILKLCSFENLSNLKVNNTGTFQLGTRKIGKHIFFRQGEVGDWRNHLTNDMVDRLNKIVEQKLSGSGLTFHDSVER</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YWJ2</accession>
    <name>A0AA38YWJ2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4110 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_022795</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9678018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YWJ2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025183">
        <property type="entry name" value="DUF4110"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13422">
        <property type="entry name" value="DUF4110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4110" evidence="2">
        <location>
            <begin position="43"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9678018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="104" mass="11892" checksum="D81678378707B707" modified="2024-01-24" version="1">MVLLLPYFSNTCHVVLMVLNRSLPMHCVLSMNAFKCVDIVSLELQELRKDGFDLAEARYRELKPILDELAILEAEQKAEEAEGLETSMRKRGNKKNSKRSAPQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YXT8</accession>
    <name>A0AA38YXT8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Cellulose synthase-like protein D3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_020618</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the glycosyltransferase 2 family. Plant cellulose synthase-like D subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YXT8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016760">
        <property type="term" value="F:cellulose synthase (UDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051753">
        <property type="term" value="F:mannan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030244">
        <property type="term" value="P:cellulose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009409">
        <property type="term" value="P:response to cold"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000229">
        <property type="entry name" value="Cellulose synthase-like protein D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000040">
        <property type="entry name" value="cellulose synthase-like protein D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005150">
        <property type="entry name" value="Cellulose_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13301">
        <property type="entry name" value="X-BOX TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03552">
        <property type="entry name" value="Cellulose_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14570">
        <property type="entry name" value="zf-RING_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="922"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="957"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1055"/>
            <end position="1077"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1083"/>
            <end position="1101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1113"/>
            <end position="1133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="420"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="852"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="384"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="390"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="391"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="420"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="612"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="613"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="637"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR605150-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR605150-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR605150-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9678477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1149" mass="128831" checksum="968CA32DE5FF5CA5" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YY66</accession>
    <name>A0AA38YY66_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Disease resistance R13L4/SHOC-2-like LRR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_020880</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YY66"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000041">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000213">
        <property type="entry name" value="Tyrosine-sulfated glycopeptide receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046956">
        <property type="entry name" value="RLP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48061:SF12">
        <property type="entry name" value="DISEASE RESISTANCE LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48061">
        <property type="entry name" value="LEUCINE-RICH REPEAT RECEPTOR PROTEIN KINASE EMS1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="929"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="14">
        <location>
            <begin position="253"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="902"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="907"/>
            <end position="923"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9678814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="979" mass="108452" checksum="7283FD15B851C739" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YY91</accession>
    <name>A0AA38YY91_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_020828</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the AP2/ERF transcription factor family. ERF subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YY91"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009873">
        <property type="term" value="P:ethylene-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000001">
        <property type="entry name" value="Ethylene-responsive transcription factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044808">
        <property type="entry name" value="ERF_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31190">
        <property type="entry name" value="DNA-BINDING DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31190:SF499">
        <property type="entry name" value="ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR ERF105"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00367">
        <property type="entry name" value="ETHRSPELEMNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0936">Ethylene signaling pathway</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="10">
        <location>
            <begin position="119"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="81"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="81"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="192"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9678752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="278" mass="30887" checksum="E54A0A667C7E0E54" modified="2024-01-24" version="1">MADKVSSFQLIHQQLLSDFECVETFVSDVGEASQASTSESSVSTSHIVQVSDYLKPNEHENNSFFLHRSTSAPSGVFQFGTKSQKTSTLSQRRPPLSISVPPPPTSQPSSAPVAGEIRHYRGVRRRPWGKFAAEIRDSNRRGSRVWLGTFETAIEAARAYDQAAFKMRGSKAILNFPLEAGNWSGSDPPDTSGRKRERDCETQVREQVEIKVLKQEEVEFPKSDSTAAAADVLGISPLTPSSWRAVWEERDMKGIFNLPPLTPSSPHHWIAYSQLLVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z018</accession>
    <name>A0AA38Z018_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fungal lipase-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_021686</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z018"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002921">
        <property type="entry name" value="Fungal_lipase-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31479">
        <property type="entry name" value="ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31479:SF2">
        <property type="entry name" value="ALPHA_BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01764">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fungal lipase-type" evidence="2">
        <location>
            <begin position="137"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9679851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="344" mass="38752" checksum="0F0BB4847D25CD31" modified="2024-01-24" version="1">MALESEDFNLTELKQLTDVDWKNNHHCRAVAAGLVQGVYKSERKRQESQGPKVQSSHWQHSHFRQKIVLTDNKDSSIYGVVYEFKHTNSIPLPECAPKFVIAFRGTFLKSGSAEQDVNLIIKLLTAELPKDNSRCKPALDAVKEVVKEDETAIIWLAGHSLGSAIAMLVGKSMAQKGKYLETFLFNPPFLRSFLSRNINSPRLENVICSTKKVIRSGISFVGGDHLWEKRHGQFNALSHWIPNLFVNKDDPLCSGYIDHFKNRKIEAEIRSIRSAFTAAFGKDRELPIHLLPQAYLTISENSSSCNFFEAHGIKQWWYQMSIGEGFADSSTKVSIGDTNLVGEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z027</accession>
    <name>A0AA38Z027_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Thaumatin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_021697</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z027"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09218">
        <property type="entry name" value="TLP-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.110.10:FF:000001">
        <property type="entry name" value="THAUMATIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.110.10">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037176">
        <property type="entry name" value="Osmotin/thaumatin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001938">
        <property type="entry name" value="Thaumatin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017949">
        <property type="entry name" value="Thaumatin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31048">
        <property type="entry name" value="OS03G0233200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00314">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002703">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00347">
        <property type="entry name" value="THAUMATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00205">
        <property type="entry name" value="THN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49870">
        <property type="entry name" value="Osmotin, thaumatin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00316">
        <property type="entry name" value="THAUMATIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51367">
        <property type="entry name" value="THAUMATIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041392782" description="Thaumatin-like protein" evidence="3">
        <location>
            <begin position="18"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="278"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="30"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="78"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="93"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="147"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="152"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="160"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="183"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="193"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR002703-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9679866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="295" mass="31021" checksum="134211539EB05AC4" modified="2024-01-24" version="1">MLSWSFTLFLIAISVLSSPSSCTFTITNNCPHTIWPGTLAGAGTPQLPTTGLQLESGQSIRIPTSPGWSGRIWARTGCTFDESGAGTCQTGDCGGRLECDGIGAAPPTSLFEITLGAGTDKDFYDVSFVDGYNLPLIAVPRGVYGGCNATGCASDINIGCPKELQVVGNSGEEGGVVACKSACDAFGLDQYCCSGEFANPSTCRPSFYSSIFKRACPRAYSYAFDDGTSTFTCKAYEYAIIFCPDANGMKRSSDTLPGPLFQGRNDGEIAGMVSSSNIILPFSLSILLLLLHLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z1W1</accession>
    <name>A0AA38Z1W1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Calcium ion-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_019788</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z1W1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37754">
        <property type="entry name" value="CALCIUM ION-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37754:SF1">
        <property type="entry name" value="CALCIUM ION-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="172"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="172"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="190"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9680578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="202" mass="23032" checksum="BC0ABDB56332E5F8" modified="2024-01-24" version="1">MGLVQSLKNRGSSVAFIPLLLMGKGLTPQKLVTLAIQRVYRNLGDYDNLEDFHSSVLSIFEIANSAFPGKHFEAPSRGQVEEVFEKWKMSDDEEERKKTFTKLLEDTKLGTIDNFTLIVGLVTPPFSMVAKRAGENVTPFKMIKNVPDALLVPTVTVLTLFSLKLIRKAAEKYSEEADYKEKRHRRNKKKREEKEDEAKARK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z2D0</accession>
    <name>A0AA38Z2D0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_020146</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9681108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z2D0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13983">
        <property type="entry name" value="STKc_WNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000075">
        <property type="entry name" value="Probable serine/threonine-protein kinase WNK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000046">
        <property type="entry name" value="probable serine/threonine-protein kinase WNK9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050588">
        <property type="entry name" value="WNK_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13902">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE WNK WITH NO LYSINE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="30"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="440"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="455"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9681108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="633" mass="71416" checksum="EEEF6A058394C12B" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z2X6</accession>
    <name>A0AA38Z2X6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PORR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_020223</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9681242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z2X6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021099">
        <property type="entry name" value="PORR_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045040">
        <property type="entry name" value="PORR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31476">
        <property type="entry name" value="PROTEIN WHAT'S THIS FACTOR 1 HOMOLOG, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31476:SF4">
        <property type="entry name" value="PROTEIN WHAT'S THIS FACTOR 1 HOMOLOG, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11955">
        <property type="entry name" value="PORR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PORR" evidence="2">
        <location>
            <begin position="87"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="422"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="459"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9681242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="530" mass="61966" checksum="AEAA54BDFD5D963C" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z492</accession>
    <name>A0AA38Z492_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Germin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_018062</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May play a role in plant defense. Probably has no oxalate oxidase activity even if the active site is conserved.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Secreted</location>
            <location evidence="2 13">Extracellular space</location>
            <location evidence="2 13">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the germin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9681994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z492"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02241">
        <property type="entry name" value="cupin_OxOx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000005">
        <property type="entry name" value="Germin-like protein subfamily 1 member 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006045">
        <property type="entry name" value="Cupin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001929">
        <property type="entry name" value="Germin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019780">
        <property type="entry name" value="Germin_Mn-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31238">
        <property type="entry name" value="GERMIN-LIKE PROTEIN SUBFAMILY 3 MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00190">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00325">
        <property type="entry name" value="GERMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00835">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00725">
        <property type="entry name" value="GERMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 13" id="KW-0052">Apoplast</keyword>
    <keyword evidence="7 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 10 13" id="KW-0464">Manganese</keyword>
    <keyword evidence="6 10 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0964">Secreted</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041489098" description="Germin-like protein" evidence="13">
        <location>
            <begin position="24"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="Cupin type-1" evidence="14">
        <location>
            <begin position="64"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="33"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU366015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9681994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="220" mass="23499" checksum="0491CD6EDA3E25F8" modified="2024-01-24" version="1">MEKGVSFLVTVALMALASSFASASDPSPLQDTCVAIDEPKNAVFVNGKFCKNPNLTVAEDFFYKGLNIPGNTSNRVGSDVTTVNVDVIPGLNTLGISLVRIDYAPYGQNPPHTHPRATEILTVLEGTLLVGFVTSNPQNRLISKVLNKGDVFVFPIGLIHFQFNIGHTNAVAIGGLSSQNPGVITIANAVFGSNPPINPDFLATAFQLDKKVVEFLQARF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z5M2</accession>
    <name>A0AA38Z5M2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Alkane hydroxylase MAH1-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_018611</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z5M2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11064">
        <property type="entry name" value="CYP86A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24296">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8 9" id="KW-0349">Heme</keyword>
    <keyword evidence="6 8 9" id="KW-0408">Iron</keyword>
    <keyword evidence="4 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="8">
        <location>
            <position position="389"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9682715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="443" mass="50148" checksum="33C429ACEBDE4CFD" modified="2024-01-24" version="1">MSPTILFQYDQVLDLGAEILERCGGTFLVIRQSLWFAHSDMLVTCIPADAHHVMSTNFWNFPKGPEFRMMLDAFGNGLFISDLEDWSFHQRIVDISLDKVENGLVPVLEHASQQRLAVDFQDLFQRFGLDATCALITGCNPKSICLELPDVPFASAMEEAGKVIFLCHVYPKCIWKLQRWLGIGSERILIAAKKTLYDFAAERVSFMRERSNMGTNQTEEGLDILSAYINEDEAFQSSASNKVLIDNILSLLLAGKDTTSAAFSWFFWLVSKNPSVETKIIEELKAVVPAKETEKWHLFDAEELSKLVYLHGALCESLRLFPPNILPSGHRLDPSTKVIFHAYAMGRMACVWGKDCLEFKPERWVTGKGRTKHEPAYKFLSFSAGPRICLGKEVVFLQMKVVVAAIMHNYHFQMVEGHRVVPTVSTVLHMKHGLKVNVKNKWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z6K9</accession>
    <name>A0AA38Z6K9_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_019097</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z6K9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36397:SF1">
        <property type="entry name" value="OS04G0482900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36397">
        <property type="entry name" value="OSJNBA0081L15.1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9683373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="126" mass="14650" checksum="D7DDB704F2E28DE9" modified="2024-01-24" version="1">MAMASNLPAYQKSRGLQIFCRKKDRERDHHPYKVIEITPPPKNLGIRCFPSINRSHHSNKFFSIQLKNLQCGESVTIEGQAYTISAVTHRYQLRKGKYEPSEKRLDVLSTGRYILNLYLENLLQQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z7M2</accession>
    <name>A0AA38Z7M2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 19">Peroxidase</fullName>
            <ecNumber evidence="4 19">1.11.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PVL29_019186</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 19">
            <text>2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:56136"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:139520"/>
            <dbReference type="ChEBI" id="CHEBI:139521"/>
            <dbReference type="EC" id="1.11.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16 19">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="16 19">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16 19">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="16 19">Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="19">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peroxidase family. Ascorbate peroxidase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.7" evidence="4 19"/>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z7M2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140825">
        <property type="term" value="F:lactoperoxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00693">
        <property type="entry name" value="secretory_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.420.10:FF:000007">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.520.10:FF:000008">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.420.10">
        <property type="entry name" value="Peroxidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000823">
        <property type="entry name" value="Peroxidase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019794">
        <property type="entry name" value="Peroxidases_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019793">
        <property type="entry name" value="Peroxidases_heam-ligand_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033905">
        <property type="entry name" value="Secretory_peroxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31517">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31517:SF11">
        <property type="entry name" value="PEROXIDASE 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00458">
        <property type="entry name" value="PEROXIDASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00461">
        <property type="entry name" value="PLPEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00435">
        <property type="entry name" value="PEROXIDASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00436">
        <property type="entry name" value="PEROXIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50873">
        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16 19" id="KW-0106">Calcium</keyword>
    <keyword evidence="12 18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 19" id="KW-0349">Heme</keyword>
    <keyword evidence="13 19" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="11 16 19" id="KW-0408">Iron</keyword>
    <keyword evidence="8 16 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 19" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6 19" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 19" id="KW-0964">Secreted</keyword>
    <keyword evidence="9 19" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041485486" description="Peroxidase" evidence="19">
        <location>
            <begin position="18"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="Plant heme peroxidase family profile" evidence="20">
        <location>
            <begin position="21"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="62"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="16">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="17">
        <location>
            <position position="58"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="31"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="64"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="118"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="197"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU362060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAJ9683503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="323" mass="35752" checksum="5B0848D4C25E0D93" modified="2024-01-24" version="1">MALLLFSLLFFLGLVNPSESRLSVNYYQKSCPRFSQIMQDTITNKQITSPTTAAATLRLFFHDCFIEGCDASVLVSSTPFNQAERDADMNLSLPGDGFDVVVRAKTALELTCPGVVSCADILAVATRDLVTMVGGPFYKVPLGRRDGLVSRANRVEGNLPRPTMSISQIISIFAVRGFSVQEMVALSGAHTIGFSHCKEFSSGIYNYSRGSQSNPSYNPRFAEGLRKACSDYQKNPTLSVFNDIMTPNKFDNMYFQNLPKGLGLLATDHTMATDPRTRQFTELYAKNQSAFFEAFGRAMEKLGLYGIKTGRRGEIRRRCDALN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z8K9</accession>
    <name>A0AA38Z8K9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Cucumisin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_016721</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the peptidase S8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z8K9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02120">
        <property type="entry name" value="PA_subtilisin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04852">
        <property type="entry name" value="Peptidases_S8_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000006">
        <property type="entry name" value="Subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.80:FF:000002">
        <property type="entry name" value="Subtilisin-like protease SBT5.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034197">
        <property type="entry name" value="Peptidases_S8_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010259">
        <property type="entry name" value="S8pro/Inhibitor_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045051">
        <property type="entry name" value="SBT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041469">
        <property type="entry name" value="Subtilisin-like_FN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10795">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17766">
        <property type="entry name" value="fn3_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05922">
        <property type="entry name" value="Inhibitor_I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 7" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041433877" description="Cucumisin" evidence="8">
        <location>
            <begin position="23"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="domain" description="Inhibitor I9" evidence="10">
        <location>
            <begin position="37"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="9">
        <location>
            <begin position="134"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="domain" description="Subtilisin-like protease fibronectin type-III" evidence="11">
        <location>
            <begin position="638"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6 7">
        <location>
            <position position="142"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6 7">
        <location>
            <position position="206"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6 7">
        <location>
            <position position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9684396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="739" mass="78411" checksum="37B42B224EC76AE5" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZAI7</accession>
    <name>A0AA38ZAI7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF4283 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_017352</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZAI7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="397"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="515"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="417"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9685292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="764" mass="84460" checksum="1F6F3F87BD909992" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZAR0</accession>
    <name>A0AA38ZAR0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">PVL29_017139</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="24">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RLP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZAR0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000309">
        <property type="entry name" value="Leucine-rich repeat receptor protein kinase MSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000221">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase PXL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000041">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000111">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000445">
        <property type="entry name" value="MDIS1-interacting receptor like kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000851">
        <property type="entry name" value="Probable leucine-rich repeat receptor-like protein kinase At1g35710"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001375">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051716">
        <property type="entry name" value="Plant_RL_S/T_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48053">
        <property type="entry name" value="LEUCINE RICH REPEAT FAMILY PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48053:SF168">
        <property type="entry name" value="LRR RECEPTOR-LIKE KINASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="16 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 22" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="725"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="791"/>
            <end position="1078"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="820"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KAJ9684989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1090" mass="120198" checksum="C5F7399ADA4F87FC" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZBB4</accession>
    <name>A0AA38ZBB4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">DNA-directed RNA polymerase subunit beta</fullName>
            <ecNumber evidence="16">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">PVL29_017349</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="23">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol III is composed of mobile elements and RPC2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft.</text>
    </comment>
    <comment type="function">
        <text evidence="14">Essential for the completion of the three rounds of mitosis in female megaspores required for the development of mature gametophytes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 16">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="16"/>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZBB4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032549">
        <property type="term" value="F:ribonucleoside binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009561">
        <property type="term" value="P:megagametogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006386">
        <property type="term" value="P:termination of RNA polymerase III transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00653">
        <property type="entry name" value="RNA_pol_B_RPB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.270.10:FF:000006">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.270.10:FF:000011">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1070.20:FF:000002">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1100.10:FF:000014">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1100.10:FF:000020">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1110.10:FF:000006">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1070.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1100.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.270.10">
        <property type="entry name" value="DNA-directed RNA polymerase, subunit 2, domain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1110.10">
        <property type="entry name" value="RNA polymerase Rpb2, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015712">
        <property type="entry name" value="DNA-dir_RNA_pol_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007120">
        <property type="entry name" value="DNA-dir_RNAP_su2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037033">
        <property type="entry name" value="DNA-dir_RNAP_su2_hyb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007121">
        <property type="entry name" value="RNA_pol_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007644">
        <property type="entry name" value="RNA_pol_bsu_protrusion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007642">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037034">
        <property type="entry name" value="RNA_pol_Rpb2_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007645">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007646">
        <property type="entry name" value="RNA_pol_Rpb2_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007647">
        <property type="entry name" value="RNA_pol_Rpb2_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014724">
        <property type="entry name" value="RNA_pol_RPB2_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20856">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04563">
        <property type="entry name" value="RNA_pol_Rpb2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04561">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04565">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04566">
        <property type="entry name" value="RNA_pol_Rpb2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04567">
        <property type="entry name" value="RNA_pol_Rpb2_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00562">
        <property type="entry name" value="RNA_pol_Rpb2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01166">
        <property type="entry name" value="RNA_POL_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 16" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 16" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 16" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 16" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RNA polymerase beta subunit protrusion" evidence="19">
        <location>
            <begin position="51"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="18">
        <location>
            <begin position="202"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="20">
        <location>
            <begin position="454"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="21">
        <location>
            <begin position="555"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="22">
        <location>
            <begin position="637"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed RNA polymerase subunit 2 hybrid-binding" evidence="17">
        <location>
            <begin position="684"/>
            <end position="1036"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00053978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00058576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU363031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF04561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF04563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF04565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF04566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF04567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAJ9685289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1073" mass="121128" checksum="E33698CE0967F471" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZH04</accession>
    <name>A0AA38ZH04_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_014513</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZH04"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36617">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36617:SF15">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase zinc-binding" evidence="1">
        <location>
            <begin position="102"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9688896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="196" mass="22746" checksum="2D9F69165A0DC8B3" modified="2024-01-24" version="1">MLTRFVVGNGDKIRFWEDLWWGDQPLGSQYPRLFRVVLDKNIPISSILGSSRPFSWNFNFRRNLLDPQIEDLETLMRSLDGLHLSPSVSDARFWPLSSSGIFSVKSFFLALSQFSGSPQVFPSKFVWKSQVPFKVRSFIWLVAHKKVNSNDMLQLRRPYKAFSPDICVLCMKHRESVDHLFLHCSLTTGYGIDYFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZH13</accession>
    <name>A0AA38ZH13_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GH18 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_014525</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 18 family. Chitinase class V subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZH13"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004568">
        <property type="term" value="F:chitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02879">
        <property type="entry name" value="GH18_plant_chitinase_class_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.10:FF:000003">
        <property type="entry name" value="Class V chitinase CHIT5b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011583">
        <property type="entry name" value="Chitinase_II/V-like_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029070">
        <property type="entry name" value="Chitinase_insertion_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001579">
        <property type="entry name" value="Glyco_hydro_18_chit_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050314">
        <property type="entry name" value="Glycosyl_Hydrlase_18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177">
        <property type="entry name" value="CHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177:SF368">
        <property type="entry name" value="GH18 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00636">
        <property type="entry name" value="Glyco_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54556">
        <property type="entry name" value="Chitinase insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01095">
        <property type="entry name" value="GH18_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="GH18" evidence="8">
        <location>
            <begin position="270"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="60"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="64"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="98"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9688911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="618" mass="65932" checksum="913A406F6C52A5EC" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZI04</accession>
    <name>A0AA38ZI04_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_014789</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9689282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZI04"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9689282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="158" mass="17870" checksum="8392C1A8C690AD40" modified="2024-01-24" version="1">MSRSHLTKFSVKNTAESSIVSLPIQAQFQEQCGNWAKTWIEGPQCMFRKQRTEITQAEPFERNAGINTDSTYPSIIQSNSEVPVMSPRKSKGEIQREPLGLKGKVLSLGRVQKKLKGAERVQLIIRGSRLSKLLQRLITKQCLGKLEFTLYAHEKANL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZIB7</accession>
    <name>A0AA38ZIB7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_012351</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9689610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZIB7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17039">
        <property type="entry name" value="Ubl_ubiquitin_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10621:SF38">
        <property type="entry name" value="UBIQUITIN DOMAIN-CONTAINING PROTEIN 7SL RNA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10621">
        <property type="entry name" value="UV EXCISION REPAIR PROTEIN RAD23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="2">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="2">
        <location>
            <begin position="117"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="2">
        <location>
            <begin position="201"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9689610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="284" mass="32219" checksum="740F277560BB9E53" modified="2024-01-24" version="1">MDVIFEPQGGEPFSLELGFFDTVLEIKEKVQKYHGIPASRQTLFFNGHLLQDNRDIPSIQMFYNCRIQMIISPDPPSETPPPLINIEDSPPPNNLQEDPSPPPPPPPLPPPPPSTKIQILLRIPTSKIHLSIEMDLAHTVGRLKEKIHEMEGIPVNRMVLYWRGRELQDSRSLRDCELSDRSEIDVGIKPLPASGSGSKRLKLMVMSKCGTKKVPVEVNPLDNVGELRKELEKLDQRFQFHLPPEGYFFIYKQNVMDDDRSFRWHHVGQGDTVEIFNGCVTGGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZL38</accession>
    <name>A0AA38ZL38_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Remorin C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_013318</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the remorin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZL38"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005516">
        <property type="entry name" value="Remorin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31471">
        <property type="entry name" value="OS02G0116800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31471:SF49">
        <property type="entry name" value="REMORIN FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03763">
        <property type="entry name" value="Remorin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Remorin C-terminal" evidence="3">
        <location>
            <begin position="400"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="77"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="286"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="466"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="19"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="35"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="107"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="291"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="306"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="333"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="468"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9691086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="512" mass="57097" checksum="44D0A8ECC9235EA9" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZL50</accession>
    <name>A0AA38ZL50_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cytochrome b561 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_013226</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZL50"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140575">
        <property type="term" value="F:transmembrane monodehydroascorbate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08760">
        <property type="entry name" value="Cyt_b561_FRRS1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045150">
        <property type="entry name" value="CYB561D1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006593">
        <property type="entry name" value="Cyt_b561/ferric_Rdtase_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15422:SF24">
        <property type="entry name" value="DOMON RELATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15422">
        <property type="entry name" value="OS05G0565100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03188">
        <property type="entry name" value="Cytochrom_B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00665">
        <property type="entry name" value="B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50939">
        <property type="entry name" value="CYTOCHROME_B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4" id="KW-0349">Heme</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041271367" description="Cytochrome b561 domain-containing protein" evidence="12">
        <location>
            <begin position="30"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="53"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="89"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="123"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="155"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="190"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b561" evidence="13">
        <location>
            <begin position="4"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9690962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="264" mass="29812" checksum="7EC2E9726D0F5C7A" modified="2024-01-24" version="1">MPIFQKLLSFTIPACFALLVLLSFVGCSSHESKGHKRINNNVHKLSPQMTFDITLHGVLLWASTGFLMPIGVLTIRMCNREECGRKVKVIFYVHVTLQVLSVLLATAGAVLSVKNFENSFNNYHQRIGLALYGAIWVQALIGFCRPGRGSKGRSVWYFVHWILGTTVSVVGMINIYTGLEAYQKKTSRSITLWTVLFTAEVCLMAFFYLFQDKWEYMQKQGVIMGNEAVTPSDQVIPTQSENSLKEILTEPCRKKNALVNQFMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZML9</accession>
    <name>A0AA38ZML9_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_013717</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZML9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32183">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32183:SF6">
        <property type="entry name" value="CYSTEINE SULFINATE DESULFINASE_CYSTEINE DESULFURASE AND RELATED ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102405">
        <property type="entry name" value="MCP/YpsA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9691610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="292" mass="32691" checksum="B99868196E8CB4B7" modified="2024-01-24" version="1">MTTPLRLVLPPLGLTSATITPIASFSFSPSKLPILFHFLTSPSPNSSNPFIFSPSLALHRCFPEKRSTLSSRQMWLCGYMRRDQDVDSSRNDEVNGVHMFGSDDEVFTQVPTQAQSVVEGSGSLLVSEYKPVSDMDYLQELLAIQQEGPRAIGFFGTRNMGFLHQELIEILSYAMVITENHIYTSGASGTNAAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSKVQNVIEKPHNDHLHLIEASRLCNLDIISRVQQVICFAFHDSKLLMETCQEARNLRKIVTLFYLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZMP4</accession>
    <name>A0AA38ZMP4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Mono-/di-acylglycerol lipase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_011153</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9691908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZMP4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00519">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002921">
        <property type="entry name" value="Fungal_lipase-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005592">
        <property type="entry name" value="Mono/diacylglycerol_lipase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46023">
        <property type="entry name" value="LIPASE CLASS 3 PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46023:SF5">
        <property type="entry name" value="OS02G0780700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03893">
        <property type="entry name" value="Lipase3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01764">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mono-/di-acylglycerol lipase N-terminal" evidence="4">
        <location>
            <begin position="47"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="Fungal lipase-type" evidence="3">
        <location>
            <begin position="182"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="421"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="421"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="431"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="444"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9691908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="666" mass="72882" checksum="F078AD10F80CAA0E" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZPV7</accession>
    <name>A0AA38ZPV7_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_011973</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9693061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZPV7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000383">
        <property type="entry name" value="Leucine-rich repeat receptor protein kinase EMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046956">
        <property type="entry name" value="RLP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063">
        <property type="entry name" value="LRR RECEPTOR-LIKE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063:SF29">
        <property type="entry name" value="LRR RECEPTOR-LIKE KINASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="232"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9693061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="296" mass="33474" checksum="BE80640E8C28C1BA" modified="2024-01-24" version="1">MSSLKQLRLRGNMLNENIPEQLCGLSDLRILDLALNNLSGSIPPCLGHLSALNSVTILDSSLDDPYDDPYREGMELVVKGQDMEFERILPIVKLIDLSSNNIWGEIPYEITNLSNLGALNLSRNQLTRKIPEDIKAMQELETLDLSCNSLSGTNPPNMSSINSLNYLDLSHNLLSGPIPIANQFQTFNDPSMVYSNLGLCELPLSTKCSTPNEDHKDEQDEEDKEDGWEMSWFFMSMGLGFPMGFWAVYGSLALNKSWRQAYFQFIDETRDRLYVFIVVNVARLRRKMERNGGAHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZQ38</accession>
    <name>A0AA38ZQ38_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">NB-ARC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_011836</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZQ38"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056789">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766:SF45">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766">
        <property type="entry name" value="PLANT BROAD-SPECTRUM MILDEW RESISTANCE PROTEIN RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25019">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NB-ARC" evidence="4">
        <location>
            <begin position="109"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="5">
        <location>
            <begin position="346"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="R13L1/DRL21-like LRR repeat region" evidence="6">
        <location>
            <begin position="586"/>
            <end position="690"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9692914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="776" mass="88496" checksum="F30DE42FE193CC0D" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZQQ3</accession>
    <name>A0AA38ZQQ3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein LURP-one-related 5-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_009467</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LOR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9693528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZQQ3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.200">
        <property type="entry name" value="LURP1-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007612">
        <property type="entry name" value="LOR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038595">
        <property type="entry name" value="LOR_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025659">
        <property type="entry name" value="Tubby-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31087">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31087:SF95">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04525">
        <property type="entry name" value="LOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54518">
        <property type="entry name" value="Tubby C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9693528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="240" mass="26397" checksum="C586CC943258DA6D" modified="2024-01-24" version="1">MSRIHPTNNKCQDLSIKGASSGVDINNAGDDAVSTNPSCPSDVLTVWKRSSMTFQGTDGFTVFDQHGRLAFRVDNYSRKKGHVKGGLVLMDGAGKALLTLKPQEQILNMQHQWNGYKGEGGCGKSLSSSKVLFSMRRRSGLIQSKQEEAEVFMGGAKKQRTTPDFRIEGSFRRRNCKIRSAVTGELVAKIARKKVNTTVLLGDDVFSLAVQPGFDSQLIMAFVMILDRICVKPFTPFLCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZS73</accession>
    <name>A0AA38ZS73_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_010033</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZS73"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000573">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF399">
        <property type="entry name" value="CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041330804" evidence="2">
        <location>
            <begin position="23"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9694335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="326" mass="36450" checksum="F4290FC1A99886DD" modified="2024-01-24" version="1">MKLHCSWMVVLATLCVAPLCFTMEPVSESDKIGSLPGQPNVSFQQFGGYVTIDEKQGRALFYYFVEAVTDPTASKPLVLWLTGGPGCSSLGYGAFMEHGPFRPRGNTLFRNKHSWNREANMLYVESPAGVGFSYSHNKSFYDDINDEVTARDNLAFLEGWFIKFPKYRNRELFIAGDSYAGHYVPQLAQLVINSGKNLNLKGILIGNPLLDFNTDMNAQGDFFWSHGLISDSTHALLTSTCNYSRIMPWVFQMTKSLSPECYEVYNKSAGEIGGSVDPFDVLADQCLSSQEVCLTDEVDVYLNRKDVQESLHAQLVGTPNWTICYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZTV3</accession>
    <name>A0AA38ZTV3_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_010266</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZTV3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000449">
        <property type="entry name" value="MYB-related transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052844">
        <property type="entry name" value="Leaf_Dev_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47214">
        <property type="entry name" value="PROTEIN ROUGH SHEATH 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47214:SF3">
        <property type="entry name" value="TRANSCRIPTION FACTOR AS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="54"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="54"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="285"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9694707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="358" mass="41286" checksum="B123CC7CE5729519" modified="2024-01-24" version="1">MKERQRWRAEEDALLRAYVRQYGPREWNLVSQRMNTPLDRDAKSCLERWKNYLRPGIKKGSLTEEEQRLVIRLQAKHGNKWKKIAAEVPGRTAKRLGKWWEVFKEKQQREQKENNKVVDPIEEGKYDRILETFAEKLVKERPAPAFLMATSNGNFLHPDPPAPPPPTLLPPWLSNSNCTSTVRPPSPSVTLSLCPSTVATSPTIPWLQPERGPDATPLVLGSLPPHGAVPTSGENLLISELVECCRELEEGHRAWAAHKKEAAWRLRRVELQLESEKACRRREKMEEIESKVKALREEQKATLDRIEAEYREQLAGLRRDAESKEQKLAEQWSAKHLRLTKFIEQMGCRPRLAEPNGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZV16</accession>
    <name>A0AA38ZV16_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Zinc finger A20 and AN1 domain-containing stress-associated protein 8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_010954</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in environmental stress response.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZV16"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1110.10:FF:000001">
        <property type="entry name" value="Zinc finger AN1-type containing 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1110.10">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035896">
        <property type="entry name" value="AN1-like_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050652">
        <property type="entry name" value="AN1_A20_ZnFinger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002653">
        <property type="entry name" value="Znf_A20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000058">
        <property type="entry name" value="Znf_AN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10634">
        <property type="entry name" value="AN1-TYPE ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10634:SF104">
        <property type="entry name" value="ZINC FINGER A20 AND AN1 DOMAIN-CONTAINING STRESS-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01754">
        <property type="entry name" value="zf-A20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01428">
        <property type="entry name" value="zf-AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00259">
        <property type="entry name" value="ZnF_A20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00154">
        <property type="entry name" value="ZnF_AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118310">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51036">
        <property type="entry name" value="ZF_A20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51039">
        <property type="entry name" value="ZF_AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="A20-type" evidence="6">
        <location>
            <begin position="12"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="AN1-type" evidence="7">
        <location>
            <begin position="107"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9695732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="172" mass="18156" checksum="61495AC6ABF58995" modified="2024-01-24" version="1">MESHDETGCQAPEGPILCINNCGFFGSAATMNMCSKCHKDLALKQEQAKLAASSIGSIVNGSSSGNGKEPIVAGTVDVQAGPVEVKAISTEASNDSSSNQIIESKVKEGPNRCTACRKRVGLTGFNCKCGNLFCAVHRYSDKHDCPFDYRTAAQDAIAKANPVVKAEKLDKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZV70</accession>
    <name>A0AA38ZV70_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase Ty1/copia-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_011067</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZV70"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase Ty1/copia-type" evidence="1">
        <location>
            <begin position="3"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9695916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="81" mass="9189" checksum="9C2828D6D87967BB" modified="2024-01-24" version="1">MLIVYVDDMIITGNDDEEITRLQNELATEFEMKNLGGLKYFMGIEVARSKQGIFISQMKYVLDILTEVGMLECKPVDAPIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZWQ8</accession>
    <name>A0AA38ZWQ8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TMEM205-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_008711</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZWQ8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025423">
        <property type="entry name" value="TMEM205-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053009">
        <property type="entry name" value="Xanthocillin_Biosynth-Assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23241">
        <property type="entry name" value="LATE EMBRYOGENESIS ABUNDANT PLANTS LEA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23241:SF102">
        <property type="entry name" value="LD23009P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13664">
        <property type="entry name" value="DUF4149"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041218705" description="TMEM205-like domain-containing protein" evidence="6">
        <location>
            <begin position="27"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="42"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="184"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="TMEM205-like" evidence="7">
        <location>
            <begin position="47"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9696631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="213" mass="23402" checksum="FA31BDAFF5945BAA" modified="2024-01-24" version="1">MGWLTRFLGALAFLAVGVILSPETFGSESDGTQSSKLTAALKLFHLLCFATAWGASLWVTFIGGIIMFKNLPRHTFGNLQSKMFPAYFSMVGVCCAVSTASFGYLHPWKSSSTAEKYQLGFLLSAFAFSLTNLLVFTPMTIKVMKQRHKVEREHNIGEEIGWSKNQEVAKVNPKLAAMNKKFGMIHGLSSLANIMSFGSLAVHSLYLSGKMDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZXG9</accession>
    <name>A0AA38ZXG9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Hexosyltransferase</fullName>
            <ecNumber evidence="11">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_008690</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Glycan metabolism; pectin biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Endomembrane system</location>
            <topology evidence="10">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the glycosyltransferase 8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="11"/>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZXG9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000024">
        <property type="entry name" value="Hexosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002495">
        <property type="entry name" value="Glyco_trans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050748">
        <property type="entry name" value="Glycosyltrans_8_dom-fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13778:SF48">
        <property type="entry name" value="GALACTURONOSYLTRANSFERASE-LIKE 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13778">
        <property type="entry name" value="GLYCOSYLTRANSFERASE 8 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01501">
        <property type="entry name" value="Glyco_transf_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00060399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9696597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="450" mass="51669" checksum="112994A0A00F779E" modified="2024-01-24" version="1">MDSSLSASSFLKGSFVPTASGLRYREKGRLIRSIFHFLSLKTLVDRRGTLGFLVSSLASLVTNPLFEFKSEKKVLGAEVYPLKMMWFMRFSGLLFAIMVMILFSPHFQSFPPAEAIRSSHHDGNLRVPIVIPAGDSPRLFSFRKASAFNNAEECGFRDRNFSGKSSVCDPFLVHVAITLDVHYLRGSMAAVHSILQHSQCPEDIFFHFLVSETHLEILVRSTFPQLKFKVYYFNPEIVRNLISTSVREALEHPLNYARNYLADLLEPCVRRVIYLDSDLIVVDDIYKLWSTSLGTRTIGAPEYCHANFTRYFTDKFWSEKRYYGTFDGRKPCYFNTGVIVIDLAKWRRFGFTKRIERWMEVQKNNRIYELGSLPPYLLVFAGHVAPIEHRWNQHGLGGDNVKGSCRELHPGPVSLLHWSGSGKPWARLDMKAPCPIDAVWSPYDLYRPLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZYG5</accession>
    <name>A0AA38ZYG5_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_009068</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZYG5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9697137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="69" mass="7603" checksum="D9B1922BABA3AABE" modified="2024-01-24" version="1">MEIQLNDCKESKAQALMKSQTKQAPPSAVFPLGRMHGFRFVIIPVASSLTVSCRPPPVSLTTMKMMLIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZYI5</accession>
    <name>A0AA38ZYI5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cytokinin dehydrogenase 1 FAD/cytokinin binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_009092</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oxygen-dependent FAD-linked oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZYI5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019139">
        <property type="term" value="F:cytokinin dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009690">
        <property type="term" value="P:cytokinin metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.462.10">
        <property type="entry name" value="FAD-linked oxidases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="entry name" value="Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016170">
        <property type="entry name" value="Cytok_DH_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015345">
        <property type="entry name" value="Cytokinin_DH_FAD/cytokin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016164">
        <property type="entry name" value="FAD-linked_Oxase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050432">
        <property type="entry name" value="FAD-linked_Oxidoreductases_BP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13878:SF127">
        <property type="entry name" value="CYTOKININ DEHYDROGENASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13878">
        <property type="entry name" value="GULONOLACTONE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09265">
        <property type="entry name" value="Cytokin-bind"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55103">
        <property type="entry name" value="FAD-linked oxidases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="Cytokinin dehydrogenase 1 FAD/cytokinin binding" evidence="7">
        <location>
            <begin position="166"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="Cytokinin dehydrogenase 1 FAD/cytokinin binding" evidence="7">
        <location>
            <begin position="285"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF09265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9697167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="336" mass="38439" checksum="0CF7076D4D6FCDD5" modified="2024-01-24" version="1">MAKSCPISTSFIIISVFISHFTLSIGLTVGLYPSSIRDFASLVNFSYNCSSPFSIAARGQGHSLRGQAMLNNHSCRGGIRVTMNPILGSYVDVGDGQLWIDVLQATLKHEWLQSPRHPHGPQISNVYEMDVITGIITNPHLFFAVLGGLDQFGIITRARIALELGPKRVKWTHMLYDEFLEFSRDQEHLISINGLDYLEGSLFDHAKYISSLISKNDIIYYLEVVKYYDELTSHMDDEELLKGLNFLIGFVFTKDVPISRFYKLNFNSGVFKDIIPKMNQTIGPLLFCDITGIKIKGYLSRYRIMEDWMNHFSQKWKNFEDRKAQFDPKMILSPRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZZW7</accession>
    <name>A0AA38ZZW7_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_007402</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic/archaeal RNase P protein component 3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZZW7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005655">
        <property type="term" value="C:nucleolar ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000044">
        <property type="entry name" value="Polymerase/histidinol phosphatase-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002738">
        <property type="entry name" value="RNase_P_p30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13031:SF0">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13031">
        <property type="entry name" value="RIBONUCLEASE P SUBUNIT P30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01876">
        <property type="entry name" value="RNase_P_p30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="PHP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="354"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="609"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="381"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9698296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="667" mass="72759" checksum="A9D6DDC6B8D9F5B8" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A3I2</accession>
    <name>A0AA39A3I2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_008043</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9699253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A3I2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009651">
        <property type="term" value="P:response to salt stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03213">
        <property type="entry name" value="ABCG_EPDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000504">
        <property type="entry name" value="ABC transporter G family member 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052215">
        <property type="entry name" value="Plant_ABCG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48042">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48042:SF15">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="376"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="404"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="448"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="486"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="517"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="608"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="25"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="650"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="653"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="667"/>
            <end position="682"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9699253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="682" mass="75933" checksum="AC08CB800F58D047" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A434</accession>
    <name>A0AA39A434_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Calcium-transporting ATPase</fullName>
            <ecNumber evidence="19">7.2.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PVL29_006011</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">Catalyzes the hydrolysis of ATP coupled with the transport of calcium.</text>
    </comment>
    <comment type="function">
        <text evidence="18">This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol out of the cell or into organelles.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17 19">
            <text>Ca(2+)(in) + ATP + H2O = Ca(2+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18105"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 19">Membrane</location>
            <topology evidence="1 19">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 19">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.10" evidence="19"/>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A434"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005388">
        <property type="term" value="F:P-type calcium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd02081">
        <property type="entry name" value="P-type_ATPase_Ca_PMCA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000039">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000006">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000013">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000018">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006408">
        <property type="entry name" value="P-type_ATPase_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01517">
        <property type="entry name" value="ATPase-IIB_Ca"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093:SF434">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE 13, PLASMA MEMBRANE-TYPE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093">
        <property type="entry name" value="CATION TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 19" id="KW-0106">Calcium</keyword>
    <keyword evidence="4 19" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="11" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="15 19" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="16 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 19" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="151"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="335"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="385"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="809"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="833"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="946"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="976"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="20">
        <location>
            <begin position="92"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00053300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU361146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAJ9700486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1012" mass="111094" checksum="330F402827469771" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A4S5</accession>
    <name>A0AA39A4S5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_006285</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A4S5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47481">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47481:SF5">
        <property type="entry name" value="RIBONUCLEASE H-LIKE DOMAIN, GAG-PRE-INTEGRASE DOMAIN, GAG-POLYPEPTIDE OF LTR COPIA-TYPE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="2">
        <location>
            <begin position="219"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9700877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="282" mass="31815" checksum="23BDD05F4AF921B0" modified="2024-01-24" version="1">MDFAPVCVKFTNTNYSTWAFQFELFLKGKDLWGHIDGTDVEKPSTFDKSQDVGSSLSWVVLDARIMSWLLGSVEPHIVIHLQPHRSAQSMWAYLKKVYHQDNDALHFQLEHAIAMFQHGSLSIQDYYSAFFTLWHEYVDLVTADVPIAALSTIQTIHTTTRYDQFLMKLSPEYESVCSSLLNRSPVPSLDIVFGELLCEEQHLSTQAILEQSYGNSGTCFCCKEYGHIAATCPKKFCSYCKKKGHIIKECRIRPQNPTSAAHDSLSGTCSVPTPPAPDYCTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A5K8</accession>
    <name>A0AA39A5K8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">PGG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_006488</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A5K8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000911">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026961">
        <property type="entry name" value="PGG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24186:SF53">
        <property type="entry name" value="PGG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24186">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13962">
        <property type="entry name" value="PGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="468"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="506"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="541"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="572"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="100"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="182"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="287"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="PGG" evidence="10">
        <location>
            <begin position="459"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="428"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="429"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9701164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="618" mass="68338" checksum="AC59CCE3ECB1BC1A" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A767</accession>
    <name>A0AA39A767_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ER lumen protein retaining receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_006447</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ERD2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A767"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046923">
        <property type="term" value="F:ER retention sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006621">
        <property type="term" value="P:protein retention in ER lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000133">
        <property type="entry name" value="ER_ret_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10585">
        <property type="entry name" value="ER LUMEN PROTEIN RETAINING RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00810">
        <property type="entry name" value="ER_lumen_recept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00660">
        <property type="entry name" value="ERLUMENR"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="105"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="136"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="161"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="193"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="234"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9701099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="282" mass="32445" checksum="513A030C2994B23A" modified="2024-01-24" version="1">MRKGGKSWLNLKWVERQPMKVKILVGVLILGFCLVGLRHLMKYDSGFPLFLASQFIHLSGIIILIFKLIKTKTCSGLSLKSQELTAIVMATRAYCSFELEEHGLTLLGSAIFLSTLWVIYMIRFKLKPTYSKELDSVPLYSLVVPPAILAVLIHPEIDHRLIRIIWPFSFYLAAVSVLPQLRLMQNAKMVEPFTAYYVFALGVARFLNCGSWIIHIYENGGRYLMFYGNWTKLWVLMVILSEFVHTFILADFCYYYLKSVMYQQLLLELSSPSYSLGRGKNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A7C7</accession>
    <name>A0AA39A7C7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">PHD finger protein ALFIN-LIKE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_004024</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Histone-binding component that specifically recognizes H3 tails trimethylated on 'Lys-4' (H3K4me3), which mark transcription start sites of virtually all active genes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Interacts with H3K4me3 and to a lesser extent with H3K4me2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">The PHD-type zinc finger mediates the binding to H3K4me3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the Alfin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A7C7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd15613">
        <property type="entry name" value="PHD_AL_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000306">
        <property type="entry name" value="PHD finger alfin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045104">
        <property type="entry name" value="Alfin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021998">
        <property type="entry name" value="Alfin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044104">
        <property type="entry name" value="PHD_AL_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12321">
        <property type="entry name" value="CPG BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12321:SF98">
        <property type="entry name" value="PHD FINGER PROTEIN ALFIN-LIKE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12165">
        <property type="entry name" value="Alfin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="13">
        <location>
            <begin position="105"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="48"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="58"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="89"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU369089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9702079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="161" mass="18098" checksum="A3B2F75477E03E21" modified="2024-01-24" version="1">MDGGPPYNPRTVEEVFRDFKGRRAGMIKALTTDVEEFYQQCDPGCNGTAKKQVKEKSSVSNHSSNKSKSNSKRGSESQGKYSKPLQAKDEDEEGLEEEEEEEHGETLCGACGENYASDEFWICCDVCEKWFHGKCVKITPARAEHIKQYKCPSCSNKRARA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A7I2</accession>
    <name>A0AA39A7I2_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_003940</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9701945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A7I2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35505">
        <property type="entry name" value="OS01G0600300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35505:SF1">
        <property type="entry name" value="SNF2 DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="489"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="511"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9701945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="521" mass="58175" checksum="FB65DD05322C0EB0" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2025-04-02" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A7V2</accession>
    <name>A0AA39A7V2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MATH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_004376</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00121">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.210.10">
        <property type="entry name" value="Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002083">
        <property type="entry name" value="MATH/TRAF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008974">
        <property type="entry name" value="TRAF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47242">
        <property type="entry name" value="TRAF-LIKE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47242:SF1">
        <property type="entry name" value="TRAF-LIKE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22486">
        <property type="entry name" value="MATH_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00061">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50144">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MATH" evidence="3">
        <location>
            <begin position="69"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="MATH" evidence="3">
        <location>
            <begin position="235"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="MATH" evidence="3">
        <location>
            <begin position="403"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="domain" description="MATH" evidence="3">
        <location>
            <begin position="571"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="852"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="911"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1410"/>
            <end position="1550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="8"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="52"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9702603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1683" mass="188059" checksum="6D6AFC180C40FA4E" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A8A7</accession>
    <name>A0AA39A8A7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Glutamate receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PVL29_004542</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Glutamate-gated receptor that probably acts as a non-selective cation channel. May be involved in light-signal transduction and calcium homeostasis via the regulation of calcium influx into cells.</text>
    </comment>
    <comment type="function">
        <text evidence="15">Glutamate-gated receptor that probably acts as non-selective cation channel.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">May form heteromers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A8A7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015276">
        <property type="term" value="F:ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13686">
        <property type="entry name" value="GluR_Plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19990">
        <property type="entry name" value="PBP1_GABAb_receptor_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000037">
        <property type="entry name" value="Glutamate receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000103">
        <property type="entry name" value="Glutamate receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000169">
        <property type="entry name" value="Glutamate receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000758">
        <property type="entry name" value="Glutamate receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000195">
        <property type="entry name" value="Glutamate receptor 2.7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044440">
        <property type="entry name" value="GABAb_receptor_plant_PBP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019594">
        <property type="entry name" value="Glu/Gly-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015683">
        <property type="entry name" value="Ionotropic_Glu_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001320">
        <property type="entry name" value="Iontro_rcpt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017103">
        <property type="entry name" value="Iontropic_Glu_rcpt_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34836">
        <property type="entry name" value="OS06G0188250 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34836:SF1">
        <property type="entry name" value="OS09G0428600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00060">
        <property type="entry name" value="Lig_chan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10613">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037090">
        <property type="entry name" value="Iontro_Glu-like_rcpt_pln"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00079">
        <property type="entry name" value="PBPe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13 15" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 15" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="9 15 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 15" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 15" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="563"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="627"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="809"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor C-terminal" evidence="19">
        <location>
            <begin position="437"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="880"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="887"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="735"/>
            <end position="791"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR037090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR037090-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAJ9702843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="953" mass="105605" checksum="493298A204105C62" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A9Y1</accession>
    <name>A0AA39A9Y1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_004958</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A9Y1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008194">
        <property type="term" value="F:UDP-glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000051">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000054">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000056">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48046:SF1">
        <property type="entry name" value="GLYCOSYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48046">
        <property type="entry name" value="UDP-GLYCOSYLTRANSFERASE 72E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9703384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="828" mass="92206" checksum="BEF15AFA4E8C806A" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AC66</accession>
    <name>A0AA39AC66_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DDB1- and CUL4-associated factor 8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_002868</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AC66"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080008">
        <property type="term" value="C:Cul4-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045151">
        <property type="entry name" value="DCAF8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15574:SF21">
        <property type="entry name" value="DDB1- AND CUL4-ASSOCIATED FACTOR 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15574">
        <property type="entry name" value="WD REPEAT DOMAIN-CONTAINING FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="50"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="440"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="440"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="505"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9704509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="529" mass="59244" checksum="8C16C8AD28566801" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ACC9</accession>
    <name>A0AA39ACC9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Ferredoxin--NADP reductase, chloroplastic</fullName>
            <shortName evidence="15">FNR</shortName>
            <ecNumber evidence="15">1.18.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PVL29_005141</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>2 reduced [2Fe-2S]-[ferredoxin] + NADP(+) + H(+) = 2 oxidized [2Fe-2S]-[ferredoxin] + NADPH</text>
            <dbReference type="Rhea" id="RHEA:20125"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.18.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 15">Plastid</location>
            <location evidence="2 15">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the ferredoxin--NADP reductase type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.18.1.2" evidence="15"/>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ACC9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004324">
        <property type="term" value="F:ferredoxin-NADP+ reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008266">
        <property type="term" value="F:poly(U) RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015979">
        <property type="term" value="P:photosynthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06208">
        <property type="entry name" value="CYPOR_like_FNR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000048">
        <property type="entry name" value="Ferredoxin--NADP reductase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.80:FF:000008">
        <property type="entry name" value="Ferredoxin--NADP reductase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015701">
        <property type="entry name" value="FNR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035442">
        <property type="entry name" value="FNR_plant_Cyanobacteria"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43314">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF501178">
        <property type="entry name" value="FNR-PetH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000361">
        <property type="entry name" value="Frd-NADP+_RD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="12" id="KW-0249">Electron transport</keyword>
    <keyword evidence="9 15" id="KW-0274">FAD</keyword>
    <keyword evidence="7 15" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10 15 16" id="KW-0521">NADP</keyword>
    <keyword evidence="13 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="8" id="KW-0934">Plastid</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FAD-binding FR-type" evidence="17">
        <location>
            <begin position="107"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="276"/>
            <end position="277"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="306"/>
            <end position="307"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="316"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="345"/>
            <end position="346"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="384"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR000361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR000361-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ9703724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="386" mass="43404" checksum="CB6D70DAFFC0D26F" modified="2024-01-24" version="1">MATAVTAAVSLPSSKTTSLSARTSIISPERISLKKVYNVYSHDWIPHQIFGQVIILFVQVPLFYRDISAGGRVVSIRAQVTTEAPAKVEKISKKNEEGVIVNKFKPKNPYIGRCLLNTKITGDDAPGETWHMVFSTEGEVPYREGQSIGVIADGVDKNGKPHKLRLYSIASSAIGDFGDSKTVSLCVKRLVYTNENGELVKGVCSNFLCDLKPGAEVKITGPVGKEMLMPKDPNATIIMLATGTGIAPFRSFLWKMFFEKHEDYKFNGLAWLFLGVPTSSSLLYKEEFEKMKEKKPENFRLDFAVSREQSNAKGEKMYIQTRMAEYAEELWELLKKDNTYVYMCGLKGMEKGIDDIMVSLAARDGIDWIEYKRQLKKAEQWNVEVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ACE4</accession>
    <name>A0AA39ACE4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">methylcrotonoyl-CoA carboxylase</fullName>
            <ecNumber evidence="5">6.4.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">3-methylcrotonyl-CoA carboxylase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">3-methylcrotonyl-CoA:carbon dioxide ligase subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_003206</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>3-methylbut-2-enoyl-CoA + hydrogencarbonate + ATP = 3-methyl-(2E)-glutaconyl-CoA + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13589"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57344"/>
            <dbReference type="ChEBI" id="CHEBI:57346"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.4.1.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid degradation; L-leucine degradation; (S)-3-hydroxy-3-methylglutaryl-CoA from 3-isovaleryl-CoA: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the AccD/PCCB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.4.1.4" evidence="5"/>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ACE4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905202">
        <property type="term" value="C:methylcrotonoyl-CoA carboxylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004485">
        <property type="term" value="F:methylcrotonoyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006552">
        <property type="term" value="P:L-leucine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000004">
        <property type="entry name" value="Methylcrotonoyl-CoA carboxylase beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000007">
        <property type="entry name" value="Methylcrotonoyl-CoA carboxylase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034733">
        <property type="entry name" value="AcCoA_carboxyl_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011763">
        <property type="entry name" value="COA_CT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011762">
        <property type="entry name" value="COA_CT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045190">
        <property type="entry name" value="MCCB/AccD1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22855">
        <property type="entry name" value="ACETYL, PROPIONYL, PYRUVATE, AND GLUTACONYL CARBOXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22855:SF13">
        <property type="entry name" value="METHYLCROTONOYL-COA CARBOXYLASE BETA CHAIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01039">
        <property type="entry name" value="Carboxyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50989">
        <property type="entry name" value="COA_CT_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50980">
        <property type="entry name" value="COA_CT_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CoA carboxyltransferase N-terminal" evidence="9">
        <location>
            <begin position="50"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="CoA carboxyltransferase C-terminal" evidence="10">
        <location>
            <begin position="312"/>
            <end position="570"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00026116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00052347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9705048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="570" mass="61903" checksum="554757FCE3A6D25F" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ADJ3</accession>
    <name>A0AA39ADJ3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclin delta-3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_003239</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ADJ3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045287">
        <property type="entry name" value="PAB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35115">
        <property type="entry name" value="CYCLIN DELTA-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35115:SF1">
        <property type="entry name" value="PROTEIN IN CHLOROPLAST ATPASE BIOGENESIS, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9705087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="339" mass="37793" checksum="9631BF30A2F20F33" modified="2024-01-24" version="1">MNVGVGVVHRPACATALPLRRGRARVQCSSSSSGHVSFIKDVAATQPPEHLHCLLKMLQTRGETVISPGARQGLIPLAIPLSQKNSGAVTALLRWPTAPPRMEMPVVEVRKHGVWLLAKNVDQYIHRLLVEEDANNSHETNGELFHASSEAGKKLYRMGDFADSQIANIDVYLLKKVGLFPDVLERKIMRHFEEGDDVSALVTGEFYTKKDLFPGFGRPFVFNAEVLLKVGRNSEAKDAARVALKSPWWTLGCKYEEVARIAQWEDEQIEYIKERVTEEGRNEDLKKGKAPAQVALDEAAFLLDLASVEGTWADSLERIAECYKEAGLNDIARFILYND</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AGG5</accession>
    <name>A0AA39AGG5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Thaumatin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_001881</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AGG5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09217">
        <property type="entry name" value="TLP-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.110.10">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037176">
        <property type="entry name" value="Osmotin/thaumatin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001938">
        <property type="entry name" value="Thaumatin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017949">
        <property type="entry name" value="Thaumatin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31048">
        <property type="entry name" value="OS03G0233200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00314">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002703">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00347">
        <property type="entry name" value="THAUMATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00205">
        <property type="entry name" value="THN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49870">
        <property type="entry name" value="Osmotin, thaumatin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00316">
        <property type="entry name" value="THAUMATIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51367">
        <property type="entry name" value="THAUMATIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041336390" description="Thaumatin-like protein" evidence="2">
        <location>
            <begin position="25"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="74"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="89"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR002703-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9706632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="155" mass="16749" checksum="38E3BCD968AD031D" modified="2024-01-24" version="1">MGLLKSLSISSFLLATLFFTSAHAAIFNIQNHCSYTVWAGAVPGGGRRLDPRQSWSLTVRAGTTGARIWARTGCNFDGAGRGHCQTGDCGGLLQCTAYGTPPNTLAEFALNQFSGKDFYDISLVDGFNVPMAFNPTSNGCTSVTYIYIYIYIYMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AI51</accession>
    <name>A0AA39AI51_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_000228</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SYG1 (TC 2.A.94) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AI51"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016036">
        <property type="term" value="P:cellular response to phosphate starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14476">
        <property type="entry name" value="SPX_PHO1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004342">
        <property type="entry name" value="EXS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052486">
        <property type="entry name" value="PHO1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034092">
        <property type="entry name" value="PHO1_SPX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48477">
        <property type="entry name" value="PHOSPHATE TRANSPORTER PHO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48477:SF1">
        <property type="entry name" value="PHOSPHATE TRANSPORTER PHO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03124">
        <property type="entry name" value="EXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03105">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51380">
        <property type="entry name" value="EXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="393"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="433"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="478"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="514"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="661"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="709"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="domain" description="SPX" evidence="11">
        <location>
            <begin position="2"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="EXS" evidence="10">
        <location>
            <begin position="599"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="180"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="210"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9708066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="790" mass="91656" checksum="5F5E91A5FD354A95" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AIA8</accession>
    <name>A0AA39AIA8_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_002568</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the amino acid-polyamine-organocation (APC) superfamily. Polyamine:cation symporter (PHS) (TC 2.A.3.12) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AIA8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015203">
        <property type="term" value="F:polyamine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000041">
        <property type="entry name" value="Amino acid permease, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044566">
        <property type="entry name" value="RMV1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45826:SF2">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45826">
        <property type="entry name" value="POLYAMINE TRANSPORTER PUT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0769">Symport</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="30"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="142"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="166"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="239"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="278"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="337"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="363"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="394"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="422"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9707592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="473" mass="51950" checksum="C906B619883AB486" modified="2024-01-24" version="1">MGECKGVEYVGVDVDEVAPLGVDNFKKVSVLPLVFLIFYEVSGGAFGTENAVSAAGPLLALVGFLIFPFIWSIPEALITAEMGTMFPENGGYVVWVSSALGPYWGFQLGWMKWFSGVIDNALYPLLFLNYLSSTVPALSGGLPRIAVALALIVVLTYMNYRGLTIVGWVAIVLGLFSILPFVIMGLLSIPKLKPSRWLVVEKDVNWSSYLTTLFWNLNYWDSISTIAGEVENPKKTLPMALFCALILVVRSYFFPLLIGTGALPLDREHWTDGYFSHVARVIGGVWLSWWITGAAAASNLGMFITEMSSDSFQLLGMAERGMLPSVFAKRSRYGTPLIGILFSASGALLFSLMSFQQTIATENFLYCFGMILEFIAFVRLRIKYPAAARPYKIPLGTFGSILMCIPPTILICIMVALSSLKVVVVSLIVVIIGLLLQPCLKCAERKKWLKFSVSSDLPDLNGENDENVVPLVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AIQ2</accession>
    <name>A0AA39AIQ2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein DETOXIFICATION</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Multidrug and toxic compound extrusion protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_000390</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AIQ2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13132">
        <property type="entry name" value="MATE_eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1410.10:FF:000011">
        <property type="entry name" value="Cyclin-D1-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.10:FF:000016">
        <property type="entry name" value="Cyclin-D1-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1410.10">
        <property type="entry name" value="I/LWEQ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.10">
        <property type="entry name" value="Talin, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049317">
        <property type="entry name" value="GCIP-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049318">
        <property type="entry name" value="GCIP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045069">
        <property type="entry name" value="MATE_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20936">
        <property type="entry name" value="GCIP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13324">
        <property type="entry name" value="GCIP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="31"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="150"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="206"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="329"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="366"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="404"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-D1-binding protein 1-like N-terminal" evidence="8">
        <location>
            <begin position="552"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-D1-binding protein 1-like C-terminal" evidence="9">
        <location>
            <begin position="719"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="693"/>
            <end position="730"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF20936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9708317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="874" mass="93845" checksum="5C8581E196192495" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AKB8</accession>
    <name>A0AA39AKB8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">S-adenosylmethionine-dependent methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_000547</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AKB8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000103">
        <property type="entry name" value="SABATH methyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.270">
        <property type="entry name" value="Methyltransferase, alpha-helical capping domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005299">
        <property type="entry name" value="MeTrfase_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042086">
        <property type="entry name" value="MeTrfase_capping"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31009">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE:CARBOXYL METHYLTRANSFERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03492">
        <property type="entry name" value="Methyltransf_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9708560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="353" mass="39446" checksum="6FBB437C55086760" modified="2024-01-24" version="1">MQQSFPMNGGDGPHSYLKNSHFQRQATIVSRTMIEEAIAKKLDVKCFSSNPFRLADLGCSVGPNTFIAMQHIVEAVERKYLAQGLNSEMPEFQVFFNDHVANDFNTLFASLPTERRYFACGVPGSFHGRLFPESSIHFMFSSHALHWLSKVPEELLDKNSPAWNRGRIHYTSGPEEVSHAYAAQFEHDMEIFLSARAKELVVGAMIVLLIPALPNGIPASQSPFGVMFDVLGASLMDMAKEGLISEAEVDSFNLPIHLASPEQMTELVERNECLIIERMELVDPRSNLVGPINGKECAMFLRAVLEGVFAQHFGSGIIDQLFDRLSKKIMESSHQLESGNKEGTLLFVVLRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ALL1</accession>
    <name>A0AA39ALL1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_001407</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ALL1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43391:SF91">
        <property type="entry name" value="OS04G0390700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43391">
        <property type="entry name" value="RETINOL DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9709908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="271" mass="30705" checksum="5CE2DAF2BEF1AA6A" modified="2024-01-24" version="1">MDLNYLIHKSMSLVIIPLVLVSLCWILPLLFLFRLIFSFFQFFSQENMAGKVVLITGASSGIGEQMAYEYAKKGACLVIVARRKKKLEEVAAQARELGSPDVVVVCGDVSNINECKQFIDEAIHHFGRLDHLVNNAGVTGGYPFEEITDITKFALLMDVNFWGTVYPTYFAIPHLRKTKGKIFVNSSSCAFLQPPRLSFYNGVFEGFPVMNTEPATKSIVDSICRGERYVTEPQWYGAIHLLKDLCPEMVEWYYSIMYFKKKSSPFAEKAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ANQ5</accession>
    <name>A0AA39ANQ5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">S-acyltransferase</fullName>
            <ecNumber evidence="8">2.3.1.225</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Palmitoyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_001493</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12 8">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="8">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="8"/>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9710045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ANQ5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF324">
        <property type="entry name" value="S-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="56"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="85"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="207"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="250"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="9">
        <location>
            <begin position="163"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9710045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="426" mass="48461" checksum="E0D87EE9803CD262" modified="2024-01-24" version="1">MQGKFYTAPLPPQLSDSNRRIIDTNTPTMRVYQIWKGSNRFFCGGRLIFGPDARSLLLTVSMIVVPVILFCAFVSQRLIDEFNHHFGNLIVGISVALTVYIMILLFLTSARDPGIIPRNPHPPEPEDDTASSISTDWVGSQSGAPNIPPTKDVMVNGMVVKVKYCQTCMLYRPPRCSHCSICNNCVDRFDHHCPWVGQCIGKRNYRFFFMFVSSTTMLCLYVFAFCWVNIERIMEAYHCSLGRAFLKSPVSGILILYTFIAAWFVGGLTAFHLYLIFTNQTTYENFRYRYDGKMNPHNLGCWRNIREIFFSKIPSSKNNFRAQVKGDSSSVFNTSMSLGHAMSPEMPKRSFDIEMGKRQAVAAEEFEDIHSQIDSIGGSERCGPQPRHTNWDHKGNWEITPDIHMLAAEFGMQHGSTVREKIHGVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D5T3</accession>
    <name>A0AA39D5T3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_025477</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D5T3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14798">
        <property type="entry name" value="RX-CC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038005">
        <property type="entry name" value="RX-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF1172">
        <property type="entry name" value="DISEASE RESISTANCE RPP13-LIKE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="4">
        <location>
            <begin position="182"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9671784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="830" mass="95217" checksum="4EBF1D7B320C201F" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D6S8</accession>
    <name>A0AA39D6S8_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_023522</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D6S8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34464:SF5">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34464">
        <property type="entry name" value="OS09G0376300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="23"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9674018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="171" mass="19222" checksum="052D75594BCF4A9D" modified="2024-01-24" version="1">MSVSLTNLSWWLWSGKHQEPKRLSNGSSLNSSQDSGLREPDALKFPLVKGANMTSTSRRTKRKWQSREERKIDREYDVVLVPSDAGCVSGSESDDSDWSIGWLEPHGPEFQSDDELDSSFAVLVPCYGRGRNDVVEDANKKIFSTIGNIPDGYSAENGKYMEQWLSSLQRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D942</accession>
    <name>A0AA39D942_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">S-acyltransferase</fullName>
            <ecNumber evidence="11">2.3.1.225</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Palmitoyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_024458</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="11"/>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D942"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF43">
        <property type="entry name" value="PALMITOYLTRANSFERASE APP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0564">Palmitate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="61"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="180"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="223"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="12">
        <location>
            <begin position="136"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9675536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="426" mass="48540" checksum="FC9CA64D7B526251" modified="2024-01-24" version="1">MAKRVYQVWQGSNKFFLHGRFIFGPDAKSLLVTLLLIIVPVTIFCALVARHLRHEFSPYNAGYAVLVAAIAFTIYVLLLLFLTSARDPGIIPRNFHPPEEEIRYDSSVEAGGRHTPSLQFPRTKEVMVNGIAVKVKYCETCMLYRPPRCSHCSICNNCVERFDHHCPWVGQCIGLRNYRFFFMFVSSSTLLCIYVFAMSAFYIKVLMDNQNDTVWKAMKESPASVILMVYCFISLWFVGGLTGFHLYLISTNQTTYENFRYRADNRINVYDRGCLNNFLEVFCTKIKPSKNKFRAYVQEEVQRPPLPSTHQAAAAAAAEDLDADTREKVEDDLGIGEDLLKISQRRNIEEIDEELCDRGSNGPRHNSSEVDSVLDSDFRSLTIQAETRHPSWGRRSSSWEIAPEVLATNSGVTESRSHVTAREANH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DAM4</accession>
    <name>A0AA39DAM4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Radical S-adenosyl methionine domain-containing protein 1, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Putative heme chaperone</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_022800</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">May be a heme chaperone, appears to bind heme. Homologous bacterial proteins do not have oxygen-independent coproporphyrinogen-III oxidase activity. Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the anaerobic coproporphyrinogen-III oxidase family. HemW subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9678023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DAM4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004109">
        <property type="term" value="F:coproporphyrinogen oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006779">
        <property type="term" value="P:porphyrin-containing compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="entry name" value="tm_1862 like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034505">
        <property type="entry name" value="Coproporphyrinogen-III_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010723">
        <property type="entry name" value="HemN_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004559">
        <property type="entry name" value="HemW-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00539">
        <property type="entry name" value="hemN_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13932">
        <property type="entry name" value="COPROPORPHYRINIGEN III OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13932:SF5">
        <property type="entry name" value="RADICAL S-ADENOSYL METHIONINE DOMAIN-CONTAINING PROTEIN 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06969">
        <property type="entry name" value="HemN_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01065">
        <property type="entry name" value="anaerobic_coproporphyrinogen-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00562">
        <property type="entry name" value="HemN-like__clustered_with_heat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Radical SAM core" evidence="6">
        <location>
            <begin position="51"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="30"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00033094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00045130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9678023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="473" mass="52506" checksum="286372F1244DEB74" modified="2024-01-24" version="1">MLKSTFTPIFSIITPKPTTPKPSKTFTKAFSLSPPTVRKNDSHNPSTTTPPHNLPPVSAYIHLPFCRKRCHYCDFPIVALGSSFTPPPEDDPRILNYTQLICREIEATKPECYPTPPLETVFFGGGTPSLVPPRLVSDIVEALRLKFGLCLDAEMSMEMDPGTFDAEKLKGLMELGVNRVSLGVQAFQEEMLRGCGRAHGVKEIYEAIETVKSCGVENWSVDLISSLPHQTPEMWEESLQLVIEAQPTHVSVYDLQVERGTKFGILYTPGEFPLPSETQSADFYRMASTALSNAGYNHYEISSYCKGGFECKHNLTYWENKPFYGFGLGSASYVGGVRFSRPRKMKEYMGYVQRLETEVVGCGGNSVDAKDVAMDVVMLSLRTARGLDLKSFRNSFGGSFVASLCKAYRPYVKSGHVVCLDEQRRPVTADEFSSLLSKDDGIEERLAFVRLSDPDGFLLSNELISLAFQAIAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DCQ0</accession>
    <name>A0AA39DCQ0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_022442</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DCQ0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052448">
        <property type="entry name" value="DnaJ_C16_autophagy_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44303">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44303:SF2">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="3">
        <location>
            <begin position="46"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="642"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="642"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="670"/>
            <end position="680"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9677452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="698" mass="78807" checksum="6D9D59A10BDA4466" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DD51</accession>
    <name>A0AA39DD51_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_021568</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DD51"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021489">
        <property type="entry name" value="DUF3143"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35765">
        <property type="entry name" value="OS05G0569200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35765:SF2">
        <property type="entry name" value="OS05G0569200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11341">
        <property type="entry name" value="DUF3143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9679676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="155" mass="17577" checksum="CB696ACFFAE3F49C" modified="2024-01-24" version="1">MSTISVSKFISVDTTLFSPKSSTINPCFFPKFPPNSKPRKSLTLVFSSSSGAELSSAAEDEWLKKLPEKKKPLYSHSLPCVEAWLRNLGFFQSKEDRAIWFVEKPEWHAQLSLDVTDLYIRYLKSGPGNLEKDVERRFSYALSREDIENAILGGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DE02</accession>
    <name>A0AA39DE02_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Plasma membrane ATPase</fullName>
            <ecNumber evidence="16">7.1.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PVL29_019786</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15 16">
            <text>ATP + H2O + H(+)(in) = ADP + phosphate + 2 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:20852"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Cell membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIIA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.1" evidence="16"/>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DE02"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008553">
        <property type="term" value="F:P-type proton-exporting transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120029">
        <property type="term" value="P:proton export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02076">
        <property type="entry name" value="P-type_ATPase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000045">
        <property type="entry name" value="ATPase 4 plasma membrane-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000004">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000004">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000211">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006534">
        <property type="entry name" value="P-type_ATPase_IIIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01647">
        <property type="entry name" value="ATPase-IIIA_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42861">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 16" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="13 16" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 16" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 16" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 16" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="66"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="101"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="248"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="282"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="650"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="715"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="802"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="833"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="17">
        <location>
            <begin position="18"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU362083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ9680576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="968" mass="106579" checksum="51A4A50E804DDD47" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DEL4</accession>
    <name>A0AA39DEL4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glycosyl transferase CAP10 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_021344</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DEL4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006598">
        <property type="entry name" value="CAP10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051091">
        <property type="entry name" value="O-Glucosyltr/Glycosyltrsf_90"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12203">
        <property type="entry name" value="KDEL LYS-ASP-GLU-LEU CONTAINING - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12203:SF99">
        <property type="entry name" value="OS04G0534100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05686">
        <property type="entry name" value="Glyco_transf_90"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00672">
        <property type="entry name" value="CAP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl transferase CAP10" evidence="1">
        <location>
            <begin position="149"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9679387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="429" mass="50286" checksum="4E75210A59A357FB" modified="2024-01-24" version="1">MSLRGLYYLQSLPTTAVEKPILPTGTAHKPLKIPKKPPVKIEYPLNCSAGYLTRTCPRNYPTAVSPEDPDRPSPPECPHYFRWIYGDLRPWMKSGITREMVERTKRTATFKLVILNGRAYVEKYQRAFQTRDVFMLWGILQLLRRYPEKVPDLELMFDCVDWPVIKSKDYHGPNATAPPPLFRYCGDDATLDIPWESLLKDLKEGNKRSKWMEREPYTYDWIRESQEGYKQSDLASQCIHRYKIYIEGSAWSVSQKYILACDSVTLLVNPHYYDFFTRSLMPVHHYWPIREDDKCRSIKFAVDWGNRHKQKAQSIGKAASDFIQEDLKMDNVYDYMFHLLNEYAMLLKFKPTVPEKAVELCSERMGCGAEGLKKKFTMESMVKYPMDASPCTMPPPFSPLELQTFLKRKVNSIKQVEAWEKKFWENQNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DF09</accession>
    <name>A0AA39DF09_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_017833</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9681626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DF09"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010017">
        <property type="term" value="P:red or far-red light signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11445">
        <property type="entry name" value="bHLH_AtPIF_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000004">
        <property type="entry name" value="Basic helix-loop-helix transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047265">
        <property type="entry name" value="PIF1-like_bHLH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044273">
        <property type="entry name" value="PIF3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46807">
        <property type="entry name" value="TRANSCRIPTION FACTOR PIF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46807:SF1">
        <property type="entry name" value="TRANSCRIPTION FACTOR PIF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="702"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="domain" description="BHLH" evidence="7">
        <location>
            <begin position="462"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="46"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="178"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="289"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="409"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="46"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="193"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="220"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="296"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="430"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="462"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9681626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="731" mass="78826" checksum="DEAE9CFE400EEB5D" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DFG2</accession>
    <name>A0AA39DFG2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Lon protease homolog, mitochondrial</fullName>
            <ecNumber evidence="11">3.4.21.53</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_017913</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10 11">ATP-dependent serine protease that mediates the selective degradation of misfolded, unassembled or oxidatively damaged polypeptides as well as certain short-lived regulatory proteins in the mitochondrial matrix. May also have a chaperone function in the assembly of inner membrane protein complexes. Participates in the regulation of mitochondrial gene expression and in the maintenance of the integrity of the mitochondrial genome. Binds to mitochondrial DNA in a site-specific manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 11">
            <text>Hydrolysis of proteins in presence of ATP.</text>
            <dbReference type="EC" id="3.4.21.53"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homohexamer or homoheptamer. Organized in a ring with a central cavity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11 12 13">Belongs to the peptidase S16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.53" evidence="11"/>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9681760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DFG2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051131">
        <property type="term" value="P:chaperone-mediated protein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070407">
        <property type="term" value="P:oxidation-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd19500">
        <property type="entry name" value="RecA-like_Lon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000021">
        <property type="entry name" value="Lon protease homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000080">
        <property type="entry name" value="Lon protease homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.5270:FF:000001">
        <property type="entry name" value="Lon protease homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1480:FF:000006">
        <property type="entry name" value="Lon protease homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.130.40:FF:000007">
        <property type="entry name" value="Lon protease homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000015">
        <property type="entry name" value="Lon protease homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.5270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.40">
        <property type="entry name" value="LON domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03120">
        <property type="entry name" value="lonm_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004815">
        <property type="entry name" value="Lon_bac/euk-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054594">
        <property type="entry name" value="Lon_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008269">
        <property type="entry name" value="Lon_proteolytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027065">
        <property type="entry name" value="Lon_Prtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003111">
        <property type="entry name" value="Lon_prtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046336">
        <property type="entry name" value="Lon_prtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027503">
        <property type="entry name" value="Lonm_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008268">
        <property type="entry name" value="Peptidase_S16_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00763">
        <property type="entry name" value="lon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43718">
        <property type="entry name" value="LON PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43718:SF2">
        <property type="entry name" value="LON PROTEASE HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05362">
        <property type="entry name" value="Lon_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22667">
        <property type="entry name" value="Lon_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02190">
        <property type="entry name" value="LON_substr_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00830">
        <property type="entry name" value="ENDOLAPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00464">
        <property type="entry name" value="LON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51787">
        <property type="entry name" value="LON_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51786">
        <property type="entry name" value="LON_PROTEOLYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01046">
        <property type="entry name" value="LON_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 11 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 11 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 11 12" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11 12" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Lon N-terminal" evidence="16">
        <location>
            <begin position="101"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="Lon proteolytic" evidence="15">
        <location>
            <begin position="786"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="704"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="741"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="active site" evidence="11 12">
        <location>
            <position position="876"/>
        </location>
    </feature>
    <feature type="active site" evidence="11 12">
        <location>
            <position position="919"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="467"/>
            <end position="474"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00050665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00058071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9681760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="981" mass="107887" checksum="9580BF2CE56E961A" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DFR4</accession>
    <name>A0AA39DFR4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Receptor-like protein 12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_019018</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DFR4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000649">
        <property type="entry name" value="Leucine Rich Repeat family protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000920">
        <property type="entry name" value="mRNA, clone: RTFL01-33-G14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046956">
        <property type="entry name" value="RLP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063">
        <property type="entry name" value="LRR RECEPTOR-LIKE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063:SF16">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041227106" description="Receptor-like protein 12" evidence="13">
        <location>
            <begin position="24"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="942"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="14">
        <location>
            <begin position="43"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="15">
        <location>
            <begin position="357"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9683258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1007" mass="111456" checksum="3F41CD8865719E5A" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DGB3</accession>
    <name>A0AA39DGB3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Omega-hydroxypalmitate O-feruloyl transferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_016395</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9683873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DGB3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050898">
        <property type="entry name" value="Plant_acyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31147">
        <property type="entry name" value="ACYL TRANSFERASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31147:SF33">
        <property type="entry name" value="N-HYDROXYCINNAMOYL_BENZOYLTRANSFERASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="197"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="197"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9683873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="440" mass="48262" checksum="C6CE3CCE8905A8CA" modified="2024-01-24" version="1">MDTNFVRVKEAVLVAPSETTPSRVLALSALDSQLFLRFSIEYLLVYQSRPCSDHGVTADHVKAALGRALVPYYPLAGRVRVGSSGSNLEVVCRAQGAVFIEAVSDLTVADFERTPRHVTQWRNLLSLYVADVLRGAPPLVVQLTWLADGGAALGVGINHCLCDGIGSAEFLNAFAELATGRRSLSEFKPKPVWDRHLLDPKPLEPPRRASSSTHPEFNSVPDLSGFVTRFSQEPLVPTSITFDKRSLNELKQLASFTGRPSESSYTSFEVLSAHVWRSWARALNMPSNQTLKLLFSINIRNRVKPSLPSGYYGNAFVLGCAQTTVKEITEKGLGHMCGLVKRAKERVGDEYVRRVVDTVSGESRASPDSVGVLILSQWSRLGLERVDFGMGRPVNVGPICSDRYCLMLPVSDQRDSVKVMVAVPTSAVDQYIHLIGSPYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DGZ1</accession>
    <name>A0AA39DGZ1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_016801</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DGZ1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37721">
        <property type="entry name" value="OS05G0464200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37721:SF1">
        <property type="entry name" value="OS05G0464200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9684503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="126" mass="13916" checksum="2748BE4AAF435059" modified="2024-01-24" version="1">MDSHWVRCHLASRLLRPYIDPTKLLGLTNTPTSLQALKTSSLLPFRLKAAMDSKSTKPVKKEKAELPPRRRGRVKAWVMGDWAETLFSIMGVQTGKTEEGEGGESSKIDNPTGESGRALPGRKIKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DI34</accession>
    <name>A0AA39DI34_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_018772</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DI34"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF78">
        <property type="entry name" value="OS12G0587133 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase zinc-binding" evidence="1">
        <location>
            <begin position="252"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9682912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="352" mass="41159" checksum="6CD5F957501396C1" modified="2024-01-24" version="1">MKRLSLWKRQYLSKGGRLTLLESTLSSLPTYFLSLFVIPKRVCARLEKIQRDFLWGGGALENRPHLVSWKVICAAKKDGGLGIRSLAIFNKALVGKWLWRFGNENDSLWKQIISRKYDIQEGGWCSKGVRDHYGVGVWKAIRNGRENFRTHSRFIVGDGTRVKFWKDLWCENQSLEDAFPNLFNLTANKEGWVAEAWEEDGVGGSWGLRFNRHLNDWEVGEVESLLSKLHPLTVRRGVDDLLWWRENKNGTFSVKSFYSSFSRGIRPPFPARIVWTPWVPIRASFFGWEVAWSRLLTIDRLKRRGWSIPNRCYLCKNEEETTDHLLLFCEKAKMLWLLIFSLFGVQWVMHSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DI83</accession>
    <name>A0AA39DI83_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_017108</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DI83"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="424"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="424"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9684954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="461" mass="33526" checksum="E5BC20A96ACDBABA" modified="2024-01-24" version="1">MLGGGGGLGGGGVGWCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGRGGGGGGGGGVGGGGGGGGGGGGGGGGWGVGGGGGGIVGGGGGGGGGLGGGGWGVGGGGGGGGGGGVGGGGGGGGGGLGGGWGGGGGGGGGGLGGGGGGGVGGGGGGGGGGGGGWGGGLGGGGGGGGWGGGGGGGGGGGGGGGLGGGCWGGGGLGELGGGGGGGGGGGLGRGLGGCGWGVGFGGGVGGWLGGVGGGWVGGGGWGWGGGGGGWGGGGGVVGVGGGWGVGGGVGGGGGWGGFGGGGGGGGGGGGVGLGLVGGGGGGAWGGGGGVGGGGGGGGGGGGGGGGGGGGGGWGGGGGGGVGGGGGGVGGVGGGGGGGVGGGGGLGVGGGGGGGGVGGWGGGGEVGGGGGVGGGLGCVLGGGGGGGGWGGGGGGGVGGGGTWGGGLGWRGGGGGPPPSPKLFFMNHARRSFWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DJI8</accession>
    <name>A0AA39DJI8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Receptor-like serine/threonine-protein kinase</fullName>
            <ecNumber evidence="19">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">PVL29_015280</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="24">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="19"/>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DJI8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048544">
        <property type="term" value="P:recognition of pollen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00028">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000384">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000370">
        <property type="entry name" value="Receptor-like protein kinase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.90.10.10:FF:000002">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.10">
        <property type="entry name" value="Bulb-type lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000858">
        <property type="entry name" value="S_locus_glycoprot_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024171">
        <property type="entry name" value="SRK-like_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47974">
        <property type="entry name" value="OS07G0415500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47974:SF19">
        <property type="entry name" value="RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00954">
        <property type="entry name" value="S_locus_glycop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000641">
        <property type="entry name" value="SRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 19" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0430">Lectin</keyword>
    <keyword evidence="13 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 19" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7 21" id="KW-0732">Signal</keyword>
    <keyword evidence="5 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041386740" description="Receptor-like serine/threonine-protein kinase" evidence="21">
        <location>
            <begin position="25"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="407"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="23">
        <location>
            <begin position="25"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="463"/>
            <end position="735"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRNR" id="PIRNR000641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KAJ9686304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="735" mass="82851" checksum="5D5CB2640103A9A1" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DKP0</accession>
    <name>A0AA39DKP0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Disease resistance RPP13-like protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_017569</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056789">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766:SF51">
        <property type="entry name" value="DISEASE RESISTANCE RPP13-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766">
        <property type="entry name" value="PLANT BROAD-SPECTRUM MILDEW RESISTANCE PROTEIN RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25019">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0611">Plant defense</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="7">
        <location>
            <begin position="28"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="6">
        <location>
            <begin position="189"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="8">
        <location>
            <begin position="445"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="domain" description="R13L1/DRL21-like LRR repeat region" evidence="9">
        <location>
            <begin position="674"/>
            <end position="795"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF25019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9685582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1351" mass="152097" checksum="108B1A331D3E0429" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DLK7</accession>
    <name>A0AA39DLK7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Probable purine permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_014549</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the purine permeases (TC 2.A.7.14) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DLK7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005345">
        <property type="term" value="F:purine nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015211">
        <property type="term" value="F:purine nucleoside transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030182">
        <property type="entry name" value="PUP_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31376">
        <property type="entry name" value="OS09G0467300 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31376:SF10">
        <property type="entry name" value="PURINE PERMEASE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16913">
        <property type="entry name" value="PUNUT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="43"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="77"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="113"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="168"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="242"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="278"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="310"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="334"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU368015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9688946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="363" mass="39995" checksum="1D198FDDD6811B0B" modified="2024-01-24" version="1">MITFHCGERMEEALPRPSVPLWEQFSNFWTRAWETYKRKPTSYWVLLILSSGAMLVAFPASSILSRLYYDNGGTSKWIISWVAVAGWPLTALMLVPTYFYFKTSPTPMNLKLVMSYIVLGFLSAADNLMYAYAYAYLPASTAALLASSSLVFSALFGYFLVNNKLNAATINAIVIITAAVTIIALDSDSDRYDNVSDSQYIMGFIWDILGSALHGLIFALSELVFVKLLGRISFHVVLEQQVMVSLFAFIFTTIGVIVEKDFQGMASEARSFKGGKASYYLVLIWGAITFQLGVLGGTAVLYLSSTVLAGVLNAIRVPLTSIAAVILLHDPMSSYKILSLIITFWGFGYYIYGSSAASKDSLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DM37</accession>
    <name>A0AA39DM37_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.143</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Beta-1,2-N-acetylglucosaminyltransferase II</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">GlcNAc-T II</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Mannoside acetylglucosaminyltransferase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="28" type="ORF">PVL29_015756</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="28 29" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="28">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>an N(4)-{beta-D-GlcNAc-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc}-L-asparaginyl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = N(4)-{beta-D-GlcNAc-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[beta-D-GlcNAc-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc}-L-asparaginyl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12941"/>
            <dbReference type="Rhea" id="RHEA-COMP:13526"/>
            <dbReference type="Rhea" id="RHEA-COMP:14369"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:60615"/>
            <dbReference type="ChEBI" id="CHEBI:60651"/>
            <dbReference type="EC" id="2.4.1.143"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 24">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyltransferase 16 (GT16) protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="28">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.143" evidence="5"/>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DM37"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005795">
        <property type="term" value="C:Golgi stack"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008455">
        <property type="term" value="F:alpha-1,6-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009312">
        <property type="term" value="P:oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007754">
        <property type="entry name" value="GlcNAc_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12871:SF0">
        <property type="entry name" value="ALPHA-1,6-MANNOSYL-GLYCOPROTEIN 2-BETA-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12871">
        <property type="entry name" value="BETA-1,2-N-ACETYLGLUCOSAMINYLTRANSFERASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05060">
        <property type="entry name" value="MGAT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 25" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16 26" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="17 24" id="KW-0464">Manganese</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 24" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="178"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="27">
        <location>
            <begin position="180"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="349"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="26">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
            <begin position="181"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
            <begin position="263"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
            <begin position="314"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00014817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00029663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00031203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00032552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00032915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00093257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRSR" id="PIRSR607754-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRSR" id="PIRSR607754-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PIRSR" id="PIRSR607754-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PIRSR" id="PIRSR607754-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="EMBL" id="KAJ9687022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="437" mass="50262" checksum="2A4F22A34AEE62BF" modified="2024-01-24" version="1">MASNKKPRIKYAALRRFLAVVLITLLGVFLLIFLLRTNSISSFVDGAQDEEVDEGYKSNDNYSHYRQILNLPKQNKLSIRLDKLNQMPPRNIDLYPNLAKDHITIVLYVHNRPQYLQVVVQSLSQVVGISETLLIVSHDGYFEEMNKIVEGIEFCQVKQIFAPYSPHVFPNSFPGVSPTDCKDKDDPRKKHCEGNPDQYGNHRSPKIVSLKHHWWWMMNTVWDGLEETRGHSGHILFIEEDHFIFPNAYRSLRLLTALKPKKCPDCYAVNLAPCDVNSKGEGWENLIAERMGNVGYAFNRTVWRKIHRKATEFCSFDEYNWDITMWATVFPSFGRPVYTLRGPRTNAVHFGKCGLHQGQGERGACIDNGSVKIDVEEIDKVVNIKPEWGVHVYKKQAGYQAGFKGWGGWGDKRDRELCLNFAYMYHLRDYPSPSATS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DMD7</accession>
    <name>A0AA39DMD7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Retrovirus-related Pol polyprotein from transposon 17.6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_014756</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9689244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DMD7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.370:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 17.6-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041373">
        <property type="entry name" value="RT_RNaseH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34072">
        <property type="entry name" value="ENZYMATIC POLYPROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34072:SF57">
        <property type="entry name" value="RNA-DIRECTED DNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17917">
        <property type="entry name" value="RT_RNaseH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="7">
        <location>
            <begin position="48"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase RNase H-like" evidence="8">
        <location>
            <begin position="221"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9689244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="459" mass="53225" checksum="B0187B3D7F7508C9" modified="2024-01-24" version="1">MQEVVQAEVLKLLQAGIIYPISDSPWVSPTQVVPKKSGIRVVQNDKGEEVSTRLTSGWRVCIDYRKLNTVTRKNHFPLPFIDQVLQRVSGHPFYYFLDGYSGYFQIEIAVEDQEKTTFICPFETYAYRRMSFGLCNTPTTFQRCMLSIFSDMVERIMEVFMDDITVYGSAFDECLVNLEAMLNQCIEKDLVLNWEKYHFMNFEELKLFLTTAPIVRAPNWQLPFEVMCDASDFAIGAVLGQREDGKPYVIYYASKTLNESQRNYTTMEKELLAVVFALDKFRAYLLGSFIVVFTDHSTLKYLLTKQDAKVRLIRWILLLQEFNLQIKDKKGLENVVADHLSRLAIAHTSHNLPINDDFLKESLMLIEVAPWYAHIANYLVIGEVPSEWKTQDKKHFFAKIHAYYWEEPFLFKYCADQIVRKCVPEQEQQGILNHCHESACGGHFASQKIAMKVLQLGFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DMK1</accession>
    <name>A0AA39DMK1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">LysM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_012769</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DMK1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00118">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.350.10">
        <property type="entry name" value="LysM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018392">
        <property type="entry name" value="LysM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052210">
        <property type="entry name" value="LysM1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036779">
        <property type="entry name" value="LysM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34997">
        <property type="entry name" value="AM15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34997:SF1">
        <property type="entry name" value="PEPTIDOGLYCAN-BINDING LYSIN DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01476">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00257">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54106">
        <property type="entry name" value="LysM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51782">
        <property type="entry name" value="LYSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0147">Chitin-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0843">Virulence</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041217741" description="LysM domain-containing protein" evidence="3">
        <location>
            <begin position="29"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="LysM" evidence="4">
        <location>
            <begin position="42"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9690278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="91" mass="9809" checksum="213E4C6146E063A1" modified="2024-01-24" version="1">MAKANKAVTAFNLVLMLSLLIFISMAESRILGAKKATPECDTVVGVESGDTCFDIAEKFQLTADFFDSINPNLNCDALFVGQWVCVDGTVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DRA9</accession>
    <name>A0AA39DRA9_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_012128</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9693249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DRA9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051238">
        <property type="entry name" value="GDSL_esterase/lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45650:SF80">
        <property type="entry name" value="FINGER PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45650">
        <property type="entry name" value="GDSL-LIKE LIPASE/ACYLHYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041366742" evidence="8">
        <location>
            <begin position="28"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9693249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="371" mass="40492" checksum="A4F13785BA0C1497" modified="2024-01-24" version="1">MKESFYCNPAICCFLFILFSVSGSVHGRHDHGQLVPCFFIFGASSFDNGNNNALPTLAKANYPPYGIDFPAGPTGRFSNGRSIVDIISEFLGFDDFIPSFASTVGGEDILKGVNYASGGSGIRAETGQHAGARISMDGQLRNHQITVLRLTNRLGQNESAAKEYLNKCIYAAGLGTNDYVSNYFLPTLYPTSRIYTPEQYALVLAQQYSQQLKTLYTNDGARKVALFGLAPLGCAPSVVASEGATNGSACVDYINDAVQIFNNRLKELVDELNRNLTDAKFIYVNVYEIASEATSCPRFKVIDAPCCPVASNNTLILCTINQTPCPNRDEYLYWDALHLSEATNMVIASRSYIAQSPTDTYPIDISDLAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DVD9</accession>
    <name>A0AA39DVD9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_007443</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DVD9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12680">
        <property type="entry name" value="RRM_THOC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000273">
        <property type="entry name" value="THO complex subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051229">
        <property type="entry name" value="ALYREF_mRNA_export"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025715">
        <property type="entry name" value="FoP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19965">
        <property type="entry name" value="RNA AND EXPORT FACTOR BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19965:SF35">
        <property type="entry name" value="RNA ANNEALING PROTEIN YRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13865">
        <property type="entry name" value="FoP_duplication"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01218">
        <property type="entry name" value="FoP_duplication"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="87"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="178"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="19"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="192"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="218"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9698363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="243" mass="25802" checksum="B339E430FC56490A" modified="2024-01-24" version="1">MSNALDMSLDDLIKNNKRSGGGGNARGRGRGSGPGPARRLPNRGANRITPYSVSKAPETTWQHDMFADQAAAYPGQAGRTSAIETGTKLYISNLDYGVSNEDIKELFSEVGDLKRYSIHYDRSGRSKGTAEVVFSRRGDAVAAVKRYNNVQLDGKPMKIEIVGTNIATPAAVPSVTNGTFGNSNGVPRSAQGRGGSQGRPRGGSGGRGFGRGRGRGRGRGEKVSAEDLDADLEKYHSEAMQIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DWM6</accession>
    <name>A0AA39DWM6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">Long-chain-fatty-acid--CoA ligase</fullName>
            <ecNumber evidence="6 7">6.2.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_008764</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoAs for both synthesis of cellular lipids, and degradation via beta-oxidation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a long-chain fatty acid + ATP + CoA = a long-chain fatty acyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15421"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57560"/>
            <dbReference type="ChEBI" id="CHEBI:83139"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Lipid metabolism; fatty acid metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.3" evidence="6 7"/>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DWM6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010143">
        <property type="term" value="P:cutin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010025">
        <property type="term" value="P:wax biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05927">
        <property type="entry name" value="LC-FACS_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045311">
        <property type="entry name" value="LC-FACS_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272:SF6">
        <property type="entry name" value="LONG CHAIN ACYL-COA SYNTHETASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 7" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="8">
        <location>
            <begin position="63"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00026121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU369030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9696712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="662" mass="74174" checksum="1EBB876BA724E909" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DY37</accession>
    <name>A0AA39DY37_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Protein ELC-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_006471</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ubiquitin-conjugating enzyme family. UEV subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DY37"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000813">
        <property type="term" value="C:ESCRT I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008333">
        <property type="term" value="P:endosome to lysosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11685">
        <property type="entry name" value="UEV_TSG101-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052070">
        <property type="entry name" value="ESCRT-I_UEV_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037202">
        <property type="entry name" value="ESCRT_assembly_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017916">
        <property type="entry name" value="SB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008883">
        <property type="entry name" value="UEV_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23306:SF3">
        <property type="entry name" value="TUMOR SUPPRESSOR PROTEIN 101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23306">
        <property type="entry name" value="TUMOR SUSCEPTIBILITY GENE 101 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05743">
        <property type="entry name" value="UEV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09454">
        <property type="entry name" value="Vps23_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140111">
        <property type="entry name" value="Endosomal sorting complex assembly domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51312">
        <property type="entry name" value="SB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51322">
        <property type="entry name" value="UEV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="5 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="UEV" evidence="11">
        <location>
            <begin position="26"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="SB" evidence="10">
        <location>
            <begin position="341"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="163"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="283"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="173"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="205"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9701138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="421" mass="46928" checksum="C6A08AB524FB17EB" modified="2024-01-24" version="1">MGGPSPSPSPSPSPNPQLTQQWLSSVLSQRGPSALPYTEDVKWLIRQHLLSLSENYPSLQPKTATYTHNDGRTVNLLQADGTVPMLYLDVTYNIPIIIWLMESYPRHPPCVYVNPTRDMVIKRPHSHVNPSGMVSIPYLHSWVYPSSNLVDLARNLSHVFSRDPPLYSQRRPNPNPNPNPNPNPTPNYNFTPNAMMGAATTVRPVIPPRAYPPSPYGSGSGTGGGRIPPSPQRHTEDPNEVFRRNAVNKLVESLHADVGVLRKTSEAEMEGMFGGQGVLRQREEQLSRGVKELQDEKEGLEQQLQMVLMNADVLEAWLRENEVKLGNMGNLDVDNAFEPCDALSKQMLDCTSSDLAIEDTIYALDKAVQEGSISFDQYLKNVRLLSREQFFHRAMCAKLRAAQMQAQVASMAARVSSQYAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E053</accession>
    <name>A0AA39E053_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Apyrase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_006977</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GDA1/CD39 NTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E053"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017110">
        <property type="term" value="F:nucleoside diphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009134">
        <property type="term" value="P:nucleoside diphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000407">
        <property type="entry name" value="GDA1_CD39_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11782">
        <property type="entry name" value="ADENOSINE/GUANOSINE DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11782:SF83">
        <property type="entry name" value="GUANOSINE-DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01150">
        <property type="entry name" value="GDA1_CD39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR600407-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9701830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="212" mass="23324" checksum="5110D6DCCB6C41FE" modified="2024-01-24" version="1">MLKRSSRQHESLSDKIYRFRGVILVISIPLLLISFVLFLMPTTTTSRSGAFQQYPLVHRKASPHSQSRTYAVIFDAGSSGSRVHVFCFDSDLNLVHIGKDLELFLQTKPGLSAYPTEPQQAAESLLPLLDKAENVVPADLRRKTPVKVGATAGLRALGIDASDRILQAVKNFLKVKSTLKSKPEWVTVLDGTQEGAFQWKSSPCQMGGGVWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E4F5</accession>
    <name>A0AA39E4F5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">protein-S-isoprenylcysteine alpha-carbonyl methylesterase</fullName>
            <ecNumber evidence="10">3.1.1.n2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_000229</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>[protein]-C-terminal S-[(2E,6E)-farnesyl]-L-cysteine methyl ester + H2O = [protein]-C-terminal S-[(2E,6E)-farnesyl]-L-cysteine + methanol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48520"/>
            <dbReference type="Rhea" id="RHEA-COMP:12125"/>
            <dbReference type="Rhea" id="RHEA-COMP:12126"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17790"/>
            <dbReference type="ChEBI" id="CHEBI:90510"/>
            <dbReference type="ChEBI" id="CHEBI:90511"/>
            <dbReference type="EC" id="3.1.1.n2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the AB hydrolase superfamily. Isoprenylcysteine methylesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.n2" evidence="10"/>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E4F5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000084">
        <property type="entry name" value="Isoprenylcysteine alpha-carbonyl methylesterase ICME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049492">
        <property type="entry name" value="BD-FAE-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050300">
        <property type="entry name" value="GDXG_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081">
        <property type="entry name" value="AB HYDROLASE SUPERFAMILY PROTEIN C4A8.06C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081:SF33">
        <property type="entry name" value="KYNURENINE FORMAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20434">
        <property type="entry name" value="BD-FAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="132"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="BD-FAE-like" evidence="13">
        <location>
            <begin position="173"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF20434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9708068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="458" mass="50522" checksum="C6863416FB7FB7BE" modified="2024-01-24" version="1">MNPPILPITNPSASSAMVATTSLTANAATMLIQEHHDETARLLIDDRAVETKPFLPRASSYTSTGTAVNGSIFYQQRRRRIASDTSLASLTTDGGGRSEYVAHAASETYLLTRLGFKLLRCLGVGYRWITRFLALGCYALLLMPGFFQVGYYYFFSSRVRRGIVYGDQPRNRLDLYLPKNSDGPKPVVAFITGGAWIIGYKAWGSLLGQQLSERDIIVACIDYRNFPQGTISDMVNDASLGVSFVCNNIAEYGGDPNRIYLMGQSAGAHIAACTLLEQAIKECGEGESTSWSVSQIKAYFGLSGGYNLFNLVDHFHSRGLYRSIFLSIMEGEQSLHRYSPEVMVQDPNIKTAVSRLPPIILFHGTADYSIPADASKSFAETLQRVGVKAESIMYEGRTHTDVFLQDPMRGGRDDMFEDLVAMIHANDPEALAKDAVAPPRKRLVPEFMLKLARAVSPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E758</accession>
    <name>A0AA39E758_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_000472</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E758"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005162">
        <property type="entry name" value="Retrotrans_gag_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33223">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33223:SF8">
        <property type="entry name" value="OS04G0172440 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03732">
        <property type="entry name" value="Retrotrans_gag"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Retrotransposon gag" evidence="2">
        <location>
            <begin position="171"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="297"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="452"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="452"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9708445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="473" mass="52730" checksum="05F8088F95E02B48" modified="2024-01-24" version="1">MSDELASTLASIQEFMAGVSRRLDQIESSRQDHPVGISTDDTVPHVPQTEQVLPPRTSHGVPFHLSDHCETAPPSAAVVSPPIVTTTDDTRLTEQEARVERLEARMRQIRLQDGGLTWDDRDGIPAAILPAKFHMPNMEPYTGIGCPRIHLRLYSTVMRAHGIDGAHLVALFPLSLSGVAQRWFASVEPSRLRTWEDVAHEFLTQFASSADIDVSRRELEATRQRPDESISSFVSRWRAKVAGMIDWPKEQDQIDMVLRNLQPRFARRLVGVPFQDLKSLVHAAFSVEEAIARGLWTDTTPSPDSKGKKPIGPFTRSGETSTVRAHLSHPQYQYQPVYVQEPYIAQTSMQSRPPHPRATTHPPPRPYAQRLARQFTPLGMTLTRAFEKLKEAGLIVPLAPRPLPQPIPPHFRLHEHCSFHQTQGHATDYCSALHHAIQDLIDSGLVNLSGPSVTTNPLPTHSTHAVPPPPSLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E7S8</accession>
    <name>A0AA39E7S8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cell cycle checkpoint protein RAD1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_002288</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the rad1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E7S8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030896">
        <property type="term" value="C:checkpoint clamp complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00577">
        <property type="entry name" value="PCNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.70.10.10:FF:000011">
        <property type="entry name" value="Damaged DNA binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.70.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046938">
        <property type="entry name" value="DNA_clamp_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003021">
        <property type="entry name" value="Rad1_Rec1_Rad17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10870">
        <property type="entry name" value="CELL CYCLE CHECKPOINT PROTEIN RAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10870:SF0">
        <property type="entry name" value="CELL CYCLE CHECKPOINT PROTEIN RAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02144">
        <property type="entry name" value="Rad1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01245">
        <property type="entry name" value="RAD1REC1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55979">
        <property type="entry name" value="DNA clamp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9707227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="299" mass="33691" checksum="DC0BC89B4E3288CD" modified="2024-01-24" version="1">MSSSGPDLVCQLDNVQGMVDALTAVRWKRHQDAVMELSEHGVVLIVEETRCLQAKVYLQRELFVQYEYSAQGRPRFGVSLGLFVDCLNTFSVPGRSNMIEIRYPGPDMQLLLKSVDSMDACIYAEIRTRIPDTISWDYNFEPAGSTPLSFTVKSAALKEAIDDLEWPGSSIQLTLEPVPPSVTFRGEGHGDLQIDFMYYVNTDLLIAFHCDHRVSYRYKYKFLRATTSNIPSSVIRDNRGSKLTIGRGGMLKVQHLVSVAKPSIPHPYVDSAGHQQPSRIAYIEFFVKPEEYEDIQNGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E801</accession>
    <name>A0AA39E801_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sucrose phosphatase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_002388</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E801"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006380">
        <property type="entry name" value="SPP-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044161">
        <property type="entry name" value="SPS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46039">
        <property type="entry name" value="SUCROSE-PHOSPHATE SYNTHASE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46039:SF1">
        <property type="entry name" value="SUCROSE-PHOSPHATE SYNTHASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05116">
        <property type="entry name" value="S6PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="171"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="Sucrose phosphatase-like" evidence="4">
        <location>
            <begin position="143"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9707362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="276" mass="31335" checksum="8C8298B73C362EC7" modified="2024-01-24" version="1">MLNNGLPIDATKNRGLVDIIEVNFRLASMVSHLTVNSLLTHMTKKGIENALLKLLANNNLDVEDIPLKFSLDGDFKINGELDVTRQKELIESLTHLASFNSNYNCYDSNGDCTERLTTIIKNMTKDVRSMVDIVEYAMCSYRRYSYRVKPGAKITLLIELMAYTKRLQMRGFLCCLVHMLIPSSLNVVPLFALRAQALRHLLVKWGIELSKMVVLVWERGDTNYEDLLVGLLNAIILRDLFQMLDMIPQDNPSITFVEEGYEAQDIFATLLALGIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YIZ8</accession>
    <name>A0AA38YIZ8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Disease resistance protein At4g27190-like leucine-rich repeats domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_025154</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YIZ8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057135">
        <property type="entry name" value="At4g27190-like_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050905">
        <property type="entry name" value="Plant_NBS-LRR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463:SF204">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23247">
        <property type="entry name" value="LRR_RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0611">Plant defense</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Disease resistance protein At4g27190-like leucine-rich repeats" evidence="3">
        <location>
            <begin position="88"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="159"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9671322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="296" mass="33839" checksum="2DA04B7D3647C7BA" modified="2024-01-24" version="1">MLEAREGGEEVCGRKVVLEGCEIKQGGRGNDDYQLVLPTNVQILEIGRCFLPTSLSDVWPSLRIAKDLKLCFILECEGIEYLWWVEDCTASLSRLWLPTLPNLRVLFKFRPTSIGSFSGLKQLEVGECHNLKHMFTPELVKYHLQNLQFIHVYVCHQIEDIAVADHDQLEEEEEEEEEEEGIHEMNNLILCFPNLRSLELEGLPELKSIWKGIMTCDSLQQIVGNFCQKLKILPLSVRINDGDGQRQASTPPLKKIIGQKEWWNGTHSHAKSFFSTLLCTTLVKGKYHICSTNPFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YJY6</accession>
    <name>A0AA38YJY6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">PVL29_025499</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="22">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048544">
        <property type="term" value="P:recognition of pollen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00028">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd01098">
        <property type="entry name" value="PAN_AP_plant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.90.10.10:FF:000004">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000195">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001019">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase B120"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.90.10.30:FF:000003">
        <property type="entry name" value="Os04g0303100 protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001270">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.10">
        <property type="entry name" value="Bulb-type lectin domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.4.10">
        <property type="entry name" value="Hepatocyte Growth Factor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021820">
        <property type="entry name" value="S-locus_recpt_kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000858">
        <property type="entry name" value="S_locus_glycoprot_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002">
        <property type="entry name" value="RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD1-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002:SF214">
        <property type="entry name" value="RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11883">
        <property type="entry name" value="DUF3403"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08276">
        <property type="entry name" value="PAN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00954">
        <property type="entry name" value="S_locus_glycop"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00473">
        <property type="entry name" value="PAN_AP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="12 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7 18" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041470494" description="non-specific serine/threonine protein kinase" evidence="18">
        <location>
            <begin position="28"/>
            <end position="1567"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="413"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1200"/>
            <end position="1223"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="20">
        <location>
            <begin position="28"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="21">
        <location>
            <begin position="313"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="472"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="20">
        <location>
            <begin position="788"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="21">
        <location>
            <begin position="1101"/>
            <end position="1183"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="1255"/>
            <end position="1532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAJ9671822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1567" mass="177329" checksum="F5FFE8563AA25478" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YLN9</accession>
    <name>A0AA38YLN9_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_026092</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YLN9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057135">
        <property type="entry name" value="At4g27190-like_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050905">
        <property type="entry name" value="Plant_NBS-LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463:SF187">
        <property type="entry name" value="AND NB-ARC DOMAIN DISEASE RESISTANCE PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23247">
        <property type="entry name" value="LRR_RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="5">
        <location>
            <begin position="57"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein At4g27190-like leucine-rich repeats" evidence="4">
        <location>
            <begin position="364"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9672738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="466" mass="54264" checksum="9867CCB1C635ECF0" modified="2024-01-24" version="1">MSVVYSIAGWRNVLNELRQHVKRHTIDMENDVFKILEFNYNHLNDEKLQECLLYCALFPEDFMIRRVSLIRYWIAEGMVEEMGSWQEERDRGHAILEKLENACLLERGLNGEYVKMHDVIKGMAINLTTKNSRFMVKIARNLEDLPSEIEWSNNVERVSLMDSHLSTLMFFSYPPKAQDEGLPNSFFVHVLSLRVLDLSHTNIAFLPYSIYDKVKLRALILCDCAKLKQVGSLAKLKELRELNLDDNQMETIPDGIEKLVHLKHFYWSSYPFYSNPLSIPLSNLVQLQCLRLADRRLPDVGVEELSGLHNSNSYMKTKHCQRLTHYRVGLNGFRFLPREKFGFLEGGKNNDDYHLVLPTNVQFFQIIKCRLSTGLLDVSQSLKMATDLKACLISRCEGIEYLWTLFLEELQSLRVLFKLTPTYIVRCSILKHLYVSKCGNLKHLFTPKLVNDHLKNLQTIHVTDCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YMK6</accession>
    <name>A0AA38YMK6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_026427</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9673139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YMK6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase zinc-binding" evidence="1">
        <location>
            <begin position="27"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9673139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="220" mass="25258" checksum="969F6D6B64409CEE" modified="2024-01-24" version="1">MRSLDGLHLSPSVPDARFWPASSSRFFSVKSFFLSLSHFSGSPQDFPSKFVWKSQVPFKVRSFIWLVAHKKVNSNDMLQLRRPYKALSPDICALCMKQGESVDHLFLHCSLTTGLWHRLFQLANLEWVTPRSIVDMMSIKFDGFGASKRGRVLWQAASTAIIWVVWKERNARIFEDRARNSENLWDSIVFLASLWAFCSKAFKGTPLNVLQLNWLAVCSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YNR7</accession>
    <name>A0AA38YNR7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transducin/WD40 repeat-like superfamily protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_023381</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YNR7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000637">
        <property type="entry name" value="WD-40 repeat family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43991:SF12">
        <property type="entry name" value="WD REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_8G05640)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43991">
        <property type="entry name" value="WD REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_8G05640)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="317"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="22"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9673797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="449" mass="50712" checksum="6C21CC3BE9E33FE4" modified="2024-01-24" version="1">MAEFQNPDLEYAVDDYYDMSDFEDDSFSETDPQRNPVDAFDSDVEDNFEMNKPKTDTSALEARNGKDIQGIPWERLNFTRDKYRETRLKQYKNYENLSRPREELEKECKKIEKGSTFYDFQFNTRLVKSTIVHFQLRNLLWATSKHDVYLMQNYSVMHWSSLLRRGKEVLNVAKPLVPTLKCTGSLGQSLSRVQISTMTVKENLMVAGGFQGELICKNLSQPGVAFCAKLTTDDNAITNAVDVYHNPNGSLRVMAANNDAEVRIFDAENFACLNRFTYPWSVNNTSVSPDGKLLAVLGDSAECLLADAQTGKVVATLKGHLDYSFASAWHPDGRTLATGNQDTTCRLWDIRNLSESQVVLKGRMGAIRAVRFSSDGRFMAMAEPADFVHVFDTESGYVKGQEIDLFGEIAGISFSPDTEALFVGIADRTYGSLLEFNRRRCNQYLDSII</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YS06</accession>
    <name>A0AA38YS06_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Calcineurin-like phosphoesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_024405</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YS06"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47680">
        <property type="entry name" value="SHEWANELLA-LIKE PROTEIN PHOSPHATASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47680:SF2">
        <property type="entry name" value="SHEWANELLA-LIKE PROTEIN PHOSPHATASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9675462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="181" mass="20282" checksum="14BDA0E285AA0DC4" modified="2024-01-24" version="1">MNVEGDFRFVTQASLDEFRAWADWFCIGDTMKSLYDGLEKSKNPFVGIHLKFLSMIPFRFGFLSQNQTVLFVGDSVFVHGGLLPNYVRDGINGLKGRFSPGHLRGRHSHVLATIPDAKRMIMGHTIQEAGINGACEIIGNSELRVLTSNPSYKDRPEVDRKCEFGLLLSKHGLRQVKVKAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YTJ4</accession>
    <name>A0AA38YTJ4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase</fullName>
            <ecNumber evidence="3">3.2.2.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_025007</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>NAD(+) + H2O = ADP-D-ribose + nicotinamide + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16301"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57967"/>
            <dbReference type="EC" id="3.2.2.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:16302"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the disease resistance TIR-NB-LRR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.6" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9676277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061809">
        <property type="term" value="F:NAD+ nucleosidase activity, cyclic ADP-ribose generating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050832">
        <property type="term" value="P:defense response to fungus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043068">
        <property type="term" value="P:positive regulation of programmed cell death"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.430:FF:000002">
        <property type="entry name" value="Disease resistance protein (TIR-NBS-LRR class)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10140:FF:000007">
        <property type="entry name" value="Disease resistance protein (TIR-NBS-LRR class)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000386">
        <property type="entry name" value="Disease resistance protein RPS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045344">
        <property type="entry name" value="C-JID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011713">
        <property type="entry name" value="Leu-rich_rpt_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058546">
        <property type="entry name" value="RPS4B/Roq1-like_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058192">
        <property type="entry name" value="WHD_ROQ1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017:SF570">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN (TIR-NBS CLASS)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20160">
        <property type="entry name" value="C-JID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23286">
        <property type="entry name" value="LRR_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07725">
        <property type="entry name" value="LRR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23282">
        <property type="entry name" value="WHD_ROQ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0611">Plant defense</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="TIR" evidence="14">
        <location>
            <begin position="20"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1209"/>
            <end position="1249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="1209"/>
            <end position="1239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00061488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9676277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1374" mass="157340" checksum="FA2721B9EC1344BC" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YTN3</accession>
    <name>A0AA38YTN3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Succinate dehydrogenase assembly factor 2, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_021820</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9676473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YTN3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006121">
        <property type="term" value="P:mitochondrial electron transport, succinate to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034553">
        <property type="term" value="P:mitochondrial respiratory chain complex II assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.250:FF:000003">
        <property type="entry name" value="Succinate dehydrogenase assembly factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.250">
        <property type="entry name" value="Flavinator of succinate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005631">
        <property type="entry name" value="SDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036714">
        <property type="entry name" value="SDH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12469">
        <property type="entry name" value="PROTEIN EMI5 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12469:SF2">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE ASSEMBLY FACTOR 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03937">
        <property type="entry name" value="Sdh5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109910">
        <property type="entry name" value="YgfY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="154"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="170"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9676473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="186" mass="21019" checksum="1C0C888836D18453" modified="2024-01-24" version="1">MGSLRRGVLGLHRILNSTAIAPRASVSATSPIPSETLIPRPRFNWICGFSSDSIHSPEIDLSNEESKRRLFNRLLYRSKQRGFLELDLVLGKWVEEHIHSMDHNGIKSLVHVLDLENPDLWKWLTGQEQPPEAVSINPVFCAVREKVMNNLNSHSAPETRAMPGKPWVRGWDDIKKGRDSPVSGNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YUR7</accession>
    <name>A0AA38YUR7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Mediator of RNA polymerase II transcription subunit 25</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_022192</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Mediator complex subunit 25 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YUR7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021419">
        <property type="entry name" value="Mediator_Med25_VWA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12433">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12433:SF11">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11265">
        <property type="entry name" value="Med25_VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mediator of RNA polymerase II transcription subunit 25 von Willebrand factor type A" evidence="3">
        <location>
            <begin position="3"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF11265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9677071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="133" mass="14528" checksum="23127DA5D8380E43" modified="2024-01-24" version="1">MGTFLQWLSAISFSGDGYDDLAIAEGLADALLMFPRHPHGTQTQQRLLGRRHCILVAASNPFPFPIPVHLPKIQNLQGAQISGATTEFSLADPNMVAKLFTQFLILISKNFPEAHAALNEYNVTSTPSIQNPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YVC0</accession>
    <name>A0AA38YVC0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Transcription factor MYB35</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_022342</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YVC0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048658">
        <property type="term" value="P:anther wall tapetum development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009555">
        <property type="term" value="P:pollen development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000204">
        <property type="entry name" value="transcription factor MYB80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000015">
        <property type="entry name" value="Transcription factor RAX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015495">
        <property type="entry name" value="Myb_TF_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47994">
        <property type="entry name" value="F14D16.11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47994:SF5">
        <property type="entry name" value="F14D16.11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="10">
        <location>
            <begin position="9"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="9">
        <location>
            <begin position="9"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="9">
        <location>
            <begin position="62"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="10">
        <location>
            <begin position="66"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="258"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="261"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9677285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="337" mass="37723" checksum="980A0AE2B0E1983D" modified="2024-01-24" version="1">MVRPPCCDKLNVKRGLWTAEEDAKILAHVSKHGTGNWTAVPKKAGLRRCGKSCRLRWTNYLRPDLKHESFTPQEEELIVRLHATIGSRWSIIAQQLPGRTDNDVKNYWNTKLRKKLSEMGIDPITHKPFSQILADYGNIGGLPKIGTRIGSLTRDLKNALMLKSDQSSAITSEEFSTSEAAQEIFLTNNRNSHDNPSMNLLTQLKAIKLVTEASSWTHHGNISSLSSPSSSSTCLTPKAKSLHTFSWSDFLLEDAFLPSDPQDQQNMSKGNLDLQSEPNADGQQQVNPIEEMTNTVLSNGSSEASSSYDSSFLEAMLDRENDMFLEFPSLLEEPFYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YYI6</accession>
    <name>A0AA38YYI6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_020995</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YYI6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000383">
        <property type="entry name" value="Leucine-rich repeat receptor protein kinase EMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001488">
        <property type="entry name" value="Receptor-like protein EIX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046956">
        <property type="entry name" value="RLP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063">
        <property type="entry name" value="LRR RECEPTOR-LIKE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063:SF101">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE FLS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041288305" description="Leucine-rich repeat-containing N-terminal plant-type domain-containing protein" evidence="13">
        <location>
            <begin position="26"/>
            <end position="1087"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1034"/>
            <end position="1057"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="14">
        <location>
            <begin position="36"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="15">
        <location>
            <begin position="354"/>
            <end position="557"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9678950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1087" mass="120523" checksum="4477D9FD767E7CDE" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z0A1</accession>
    <name>A0AA38Z0A1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_021723</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z0A1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000234">
        <property type="entry name" value="WD repeat-containing protein LWD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045159">
        <property type="entry name" value="DCAF7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19919">
        <property type="entry name" value="WD REPEAT CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="166"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="255"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9679909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="336" mass="37754" checksum="00B032C0939F2E8D" modified="2024-01-24" version="1">MERSSQESHLRSENCVTYESPYPLYSMAISSSTQHNHPRIAIGSFIEEVSNRVDIVSFDEDALAIRTHPSLSFDHPYPPTKLMFHPNSRTSPDHLASSGEYLRLWEVRDNSIQPLSVLNNSKTSEFCAPLTSFDWNEVEPRRIGTSSIDTTCTIWDVERGVVETQLIAHDKEVYDIAWGEAGVFASVSADGSVRIFDLRDKEHSTIIYESPQPDTPLLRLAWNKQDVRYMATILMDSNKIVILDIRSPTMPVAELERHRASVNAISWAPQSSRHICSGGDDSQALIWELPTLAGPNGIDPMSVYLASSEINQLQWSAAQPEWIAIAFSNKLQLLKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z0K0</accession>
    <name>A0AA38Z0K0_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_021753</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the peptidase S8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z0K0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02120">
        <property type="entry name" value="PA_subtilisin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04852">
        <property type="entry name" value="Peptidases_S8_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.2310:FF:000002">
        <property type="entry name" value="p69E protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000005">
        <property type="entry name" value="subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.80:FF:000002">
        <property type="entry name" value="Subtilisin-like protease SBT5.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034197">
        <property type="entry name" value="Peptidases_S8_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010259">
        <property type="entry name" value="S8pro/Inhibitor_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045051">
        <property type="entry name" value="SBT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041469">
        <property type="entry name" value="Subtilisin-like_FN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10795">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17766">
        <property type="entry name" value="fn3_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05922">
        <property type="entry name" value="Inhibitor_I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 7" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041354125" evidence="8">
        <location>
            <begin position="28"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="domain" description="Inhibitor I9" evidence="11">
        <location>
            <begin position="37"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="9">
        <location>
            <begin position="141"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="10">
        <location>
            <begin position="362"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="Subtilisin-like protease fibronectin type-III" evidence="12">
        <location>
            <begin position="669"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6 7">
        <location>
            <position position="150"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6 7">
        <location>
            <position position="200"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6 7">
        <location>
            <position position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF05922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF17766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9679952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="774" mass="83282" checksum="B245A67D6C0FBF43" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z0Q2</accession>
    <name>A0AA38Z0Q2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TIR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_019523</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z0Q2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32009:SF121">
        <property type="entry name" value="SIMILAR TO PART OF DISEASE RESISTANCE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32009">
        <property type="entry name" value="TMV RESISTANCE PROTEIN N-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0520">NAD</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TIR" evidence="2">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9680238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="66" mass="7581" checksum="99D927F9C66AA502" modified="2024-01-24" version="1">MECQKDLGHAVFPIFYHVDPSHVRKQEGSFGEAFAGYEENWKDKIPRWRTALTEAANLSGWDLQDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z150</accession>
    <name>A0AA38Z150_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_019461</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z150"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041292832" evidence="1">
        <location>
            <begin position="16"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9680173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="118" mass="13135" checksum="B40499E5900F9A6A" modified="2024-01-24" version="1">MISLTLGTALLITLSQNVTVMAPVSLGIAFPSGISSMETKFQEMDQMHLDFQILMLIISNLLTTTDNFLTEWEFIPAYHYQLAVHHLKEKQSCLEFALLMTESAGATKQCLVPTTVNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z1R7</accession>
    <name>A0AA38Z1R7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein ECERIFERUM 1-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_019648</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sterol desaturase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z1R7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021940">
        <property type="entry name" value="CER1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006694">
        <property type="entry name" value="Fatty_acid_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050307">
        <property type="entry name" value="Sterol_Desaturase_Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11863">
        <property type="entry name" value="STEROL DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12076">
        <property type="entry name" value="CER1-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04116">
        <property type="entry name" value="FA_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="18"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="126"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="182"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid hydroxylase" evidence="8">
        <location>
            <begin position="133"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="Very-long-chain aldehyde decarbonylase CER1-like C-terminal" evidence="9">
        <location>
            <begin position="451"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="476"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9680384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="620" mass="71557" checksum="419C9A08E8255506" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z347</accession>
    <name>A0AA38Z347_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein yippee-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_020144</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the yippee family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9681104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z347"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034751">
        <property type="entry name" value="Yippee"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004910">
        <property type="entry name" value="Yippee/Mis18/Cereblon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039058">
        <property type="entry name" value="Yippee_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13848">
        <property type="entry name" value="PROTEIN YIPPEE-LIKE CG15309-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03226">
        <property type="entry name" value="Yippee-Mis18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51792">
        <property type="entry name" value="YIPPEE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Yippee" evidence="5">
        <location>
            <begin position="8"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU110713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9681104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="106" mass="12219" checksum="631BCC08E4C5085F" modified="2024-01-24" version="1">MADVSAPRIYSCCNCRNHVARHEDIISKNFQASHGRAFLFSHAMNIVEGPKENRNLITGLHVVADIYCKNCGMALGWKYDKAYEEAQKYKEGKFVLEKFKIMKENF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z4J9</accession>
    <name>A0AA38Z4J9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Galactose oxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_018238</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z4J9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02851">
        <property type="entry name" value="E_set_GO_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.80">
        <property type="entry name" value="Galactose oxidase/kelch, beta-propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011043">
        <property type="entry name" value="Gal_Oxase/kelch_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037293">
        <property type="entry name" value="Gal_Oxidase_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009880">
        <property type="entry name" value="Glyoxal_oxidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015202">
        <property type="entry name" value="GO-like_E_set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32208:SF71">
        <property type="entry name" value="GLYOXAL OXIDASE-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32208">
        <property type="entry name" value="SECRETED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07250">
        <property type="entry name" value="Glyoxal_oxid_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09118">
        <property type="entry name" value="GO-like_E_set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50965">
        <property type="entry name" value="Galactose oxidase, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Glyoxal oxidase N-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="Galactose oxidase-like Early set" evidence="3">
        <location>
            <begin position="394"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9682263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="498" mass="55657" checksum="36BEFE6ABE64A49A" modified="2024-01-24" version="1">MQLLWNNKVVIFDRTDFGPSNLSLPPHLCRHDPRDLALEIDCTAHSLLYCVDTNTFRPLKVQTDTWCSSGSVLPNGTLTQTGGFNDGDHVLRMFTPCDDYNCDWVEFPGYLSQRRWYASNQILPDGRIIIVGGRQQFNYEFYPRSSRGSNLYTLDFLRETRDANENNLYPFLHLLPDGNLFIFANTRSISLDYKRNRVVQEFPPITGGEPRNYPSSGSSVLLPINESQAIEAEVLVCGGAPPGAVSQALRGNFVSASLTCGRLKVTDENPNWVMEEMPVARVMGDMLLLPTGDVIIINGARLGTAGWEYGRSPVTNSVIYRPFGDVNQRFLVMSGSIRPRMYHSSAVLLPDGRILVGGSNPHIYYNFTNVLYPTDLSLEAYSPPYTSPAFSSLRPHILSQDENLRYGQSFSVVFNVCEYTNDRVLSANIVAPSFTTHTVAMNQRMVVLRVESVMQEAGNRYRLSVVGPSTPEIAPPGYYMLFVVHSGIPSPGTWVKIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z562</accession>
    <name>A0AA38Z562_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GAG-pre-integrase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_018499</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z562"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025724">
        <property type="entry name" value="GAG-pre-integrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054722">
        <property type="entry name" value="PolX-like_BBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47592:SF27">
        <property type="entry name" value="OS08G0421700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47592">
        <property type="entry name" value="PBF68 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13976">
        <property type="entry name" value="gag_pre-integrs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22936">
        <property type="entry name" value="Pol_BBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Retrovirus-related Pol polyprotein from transposon TNT 1-94-like beta-barrel" evidence="3">
        <location>
            <begin position="122"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="GAG-pre-integrase" evidence="2">
        <location>
            <begin position="214"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9682590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="307" mass="34368" checksum="B76787F3FAF77872" modified="2024-01-24" version="1">MLDLSFRPEASALLTRGPHAAESSGPSPRWSKRTYCEYCKKLGHIKDTCWALHGKPIDWKPRQPNKGHNHQASTETQADKTPTKICQSTSSVGFNSDQLAKLYELFSNFQASAYTMSQITPWIIDSGASNHMTDAHHLFSIYSPYAGNLKVKIADGTLSPVAGKGSIRISESITLNPILDVPNLSCNLLSISQLTKKSNCSAKFLLSHLLRNMGLYYFDEIDVHGQCPPTVCNSASHPKDSELLLWHKRMSHPSFQYLKHLFPSLCSNKTSLDFQCEVCELAKHHRASFPKSKYKAYIPFTLIHSDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z5L5</accession>
    <name>A0AA38Z5L5_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_018551</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z5L5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="139"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9682652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="163" mass="18388" checksum="805F11C5E076BF25" modified="2024-01-24" version="1">MATNLRARFRERQHKRLSDYIVISPSPSKKACPTPRPNSLSMPTLSTPTDAITSGLNEKPSSVCNIPYHEMRKPFLISGSINEESFECPHLSPHCPKTTYVPSREKMFELLRCFLSFTENEPPMQDMGTLFSATQSSPFFSLVTFLVFPLVSCFIMIFAASRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z8C3</accession>
    <name>A0AA38Z8C3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DUF3741 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_016552</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z8C3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022212">
        <property type="entry name" value="DUF3741"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032795">
        <property type="entry name" value="DUF3741-assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46836">
        <property type="entry name" value="AFADIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46836:SF7">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL N-ACETYGLUCOSAMINLYTRANSFERASE SUBUNIT P-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12552">
        <property type="entry name" value="DUF3741"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14383">
        <property type="entry name" value="VARLMGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF3741" evidence="3">
        <location>
            <begin position="9"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="domain" description="DUF3741" evidence="2">
        <location>
            <begin position="105"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="250"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="456"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="500"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="675"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="256"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="462"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="508"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="675"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="687"/>
            <end position="698"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9684110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="735" mass="80655" checksum="A373A6E3B93225B8" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z9P1</accession>
    <name>A0AA38Z9P1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PVL29_017040</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.10.10:FF:000007">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 17.6-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.370:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 17.6-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000020">
        <property type="entry name" value="Transposon Tf2-6 polyprotein-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005162">
        <property type="entry name" value="Retrotrans_gag_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041373">
        <property type="entry name" value="RT_RNaseH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056924">
        <property type="entry name" value="SH3_Tf2-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03732">
        <property type="entry name" value="Retrotrans_gag"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17917">
        <property type="entry name" value="RT_RNaseH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08284">
        <property type="entry name" value="RVP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24626">
        <property type="entry name" value="SH3_Tf2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="11" id="KW-0229">DNA integration</keyword>
    <keyword evidence="15" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="13" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="8" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="18">
        <location>
            <begin position="623"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase catalytic" evidence="19">
        <location>
            <begin position="1169"/>
            <end position="1334"/>
        </location>
    </feature>
    <feature type="domain" description="Chromo" evidence="17">
        <location>
            <begin position="1479"/>
            <end position="1531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="246"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="319"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="8"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="35"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="319"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="359"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAJ9684857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1531" mass="176373" checksum="5D6021336B222DF3" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z9T8</accession>
    <name>A0AA38Z9T8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_017198</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z9T8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9685071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="147" mass="17620" checksum="DB266D1C3620D28C" modified="2024-01-24" version="1">MICLVGKELQSFFLHVYVLPKKKKKTKRQIQWFGLTITNISLSIFSLVLHRFGGITLLRLYFYKQNIYLGRLRTAKNVSNARMQNSKLIEDLIKMFTGLKFYENIQIMTRFLNLDQLFQISFLHIPFFSFFLFLISSSTFHLRCWFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZA56</accession>
    <name>A0AA38ZA56_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_016911</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZA56"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF78">
        <property type="entry name" value="OS12G0587133 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="440"/>
            <end position="720"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9684674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="980" mass="112409" checksum="42586B12B781CDAA" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZB10</accession>
    <name>A0AA38ZB10_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glycerol-3-phosphate dehydrogenase [NAD(+)]</fullName>
            <ecNumber evidence="8">1.1.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_017536</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6 8">
            <text>sn-glycerol 3-phosphate + NAD(+) = dihydroxyacetone phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11092"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.8" evidence="8"/>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZB10"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141152">
        <property type="term" value="F:glycerol-3-phosphate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046168">
        <property type="term" value="P:glycerol-3-phosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.10:FF:000012">
        <property type="entry name" value="Glycerol-3-phosphate dehydrogenase [NAD(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000109">
        <property type="entry name" value="Glycerol-3-phosphate dehydrogenase [NAD(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000229">
        <property type="entry name" value="Glycerol-3-phosphate dehydrogenase [NAD(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006168">
        <property type="entry name" value="G3P_DH_NAD-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006109">
        <property type="entry name" value="G3P_DH_NAD-dep_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011128">
        <property type="entry name" value="G3P_DH_NAD-dep_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11728">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11728:SF33">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE DEHYDROGENASE [NAD(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07479">
        <property type="entry name" value="NAD_Gly3P_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01210">
        <property type="entry name" value="NAD_Gly3P_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00077">
        <property type="entry name" value="GPDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7" id="KW-0520">NAD</keyword>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycerol-3-phosphate dehydrogenase NAD-dependent N-terminal" evidence="10">
        <location>
            <begin position="148"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="Glycerol-3-phosphate dehydrogenase NAD-dependent C-terminal" evidence="11">
        <location>
            <begin position="276"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="7"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9685535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="465" mass="51807" checksum="446B7E448517229F" modified="2024-01-24" version="1">MVGTTEGMANSGYSNGSVQNSNGVQEEKLDELRRLIGKAEGDPMRIVGVGAGAWGSVFAAMLQDNYGHLRDKVQIRIWRRPGRFVDRATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLHADEILKDGFCLNMIDTPLCPMKVVTNLQEAVWDADIVINGLPSTETREVFEEISKYWKERITVPIIISLAKGIEAELEPEPRVITPTQMINRATGVPMENILYLGGPNIASEIYNKEYANARICGAEKWRKPLAKFLRQPHFIVWDNGDLITHEVMGGLKNAYAIGAGMVAALTNESATSKSVYFAHCTSEMIFITHLLSEDPEKLSGPLLADTYVTLLKGRNAWYGQKLADGELSLEMGDSIKGKGMIQGISAVKAFYELLSQSCLSILHSEENKPVAPVELCPILKTLYNILIAREASSQAILQALRDQTMNDPRDRIAMAQTHAFYRPSLLGQQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZDE8</accession>
    <name>A0AA38ZDE8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AUGMIN subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_015431</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZDE8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990498">
        <property type="term" value="C:mitotic spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031023">
        <property type="term" value="P:microtubule organizing center organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051225">
        <property type="term" value="P:spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028346">
        <property type="entry name" value="HAUS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16039">
        <property type="entry name" value="HAUS AUGMIN-LIKE COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16039:SF1">
        <property type="entry name" value="HAUS AUGMIN-LIKE COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15003">
        <property type="entry name" value="HAUS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="216"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="267"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9686532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="304" mass="34027" checksum="EB9F1C7D2161DFCB" modified="2024-01-24" version="1">MSMGSDTSWVGKKPLRRLGGMSDALSIAADLGFSVNPPPSQEELQNLSSTGEKGDDLIRVLRELTTVQRKIADLQVELQGRKEDKNVAHLTHVSEMEKKCETLARITAILKDVIQNKDRIIARLQQPYSLDCIPVEAEFQKQFSELLMKAASDYGALTASVADFQWTQNFKEPPSVWGEMLRPIPVALASCTRFFEAMSAMRESFATLQNLRVGNSASSLPTTPAKDPYQRIPGDSECVTPPPWRNEPGFDDLAVRSVRRQENERQEVEDENSEEGEFNQVDGTSHRRLSWPPSVKKSDGKSLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZDF4</accession>
    <name>A0AA38ZDF4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Polygalacturonase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_015625</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the glycosyl hydrolase 28 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZDF4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004650">
        <property type="term" value="F:polygalacturonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000142">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000152">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000743">
        <property type="entry name" value="Glyco_hydro_28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31375">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00295">
        <property type="entry name" value="Glyco_hydro_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00502">
        <property type="entry name" value="POLYGALACTURONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0134">Cell wall</keyword>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6 9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041465429" description="Polygalacturonase" evidence="10">
        <location>
            <begin position="25"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9686854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="401" mass="43236" checksum="EC103F919328DE80" modified="2024-01-24" version="1">MGLKLNITATSLLLLLASAAEVACDTIFYVTKYGERADGNTDISQALLKAWGDACSSPVASTVMTPDGTYALGQITIGGPCKAPINFIVQGTVKAPMDTRRFKAEAGWIAFQQIDQFTLSGGGVFDGQGKTVWGTKCPSSAYCNQLPILSSCIYMLMFNFITNSMVKDITSRDSKQFHINLLGCKNLTFYNVVISAPEESLNTDGIHIGRSSGINITDSTIETGDDCVSIGDGSEQINMQRVTCGPGHGISVGSLGKYPNEEPVVGISVKNRTLTNTQNGVRVKTWPASHQGTAYEMHFEDIVMNNVGNPIIIDQEHCPHNQCNLKIPSRIKLSSVSFRNIRGTTSTQVAVKLVCSQGVPCEDVELGDINLKYKGNEGHAMSQCKTIKPNLLGTQLPRTRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZEJ6</accession>
    <name>A0AA38ZEJ6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_016168</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZEJ6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.60">
        <property type="entry name" value="YhbY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040286">
        <property type="entry name" value="At3g25440-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001890">
        <property type="entry name" value="RNA-binding_CRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035920">
        <property type="entry name" value="YhbY-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31426">
        <property type="entry name" value="GROUP II INTRON SPLICING FACTOR CRS1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31426:SF5">
        <property type="entry name" value="OS04G0492900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01985">
        <property type="entry name" value="CRS1_YhbY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01103">
        <property type="entry name" value="CRS1_YhbY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75471">
        <property type="entry name" value="YhbY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51295">
        <property type="entry name" value="CRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="CRM" evidence="4">
        <location>
            <begin position="41"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="251"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="275"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="288"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9687571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="331" mass="37999" checksum="A6FE222DBC636E87" modified="2024-01-24" version="1">MTAEEKILYKLRKARRKEERLAEALTKIEPKESSETTHDPEILTPEEHFFFLKMGLKCKNYVPIGRRGIYQGVILNMHLHWKKHQTLKVVVKTFSLEEVKEIAVELARLTGGIVLDIHEENTIIMYRGKNYSQPPTEIMSPRITLSRKKALDKSKYRDGLRAVRKYIPRLEGDLELLQAQAKMQAENKTEAVEDFQNANIDSINSQGISNLQPENSDKLRELLAGNNESSEEESLTDSGTESCSEALSDIFESDSDTDTKEKAEQPLYLNEFEKFPVESDGEPEDFEEHLRQISADSRKAKSLEKDADLPQLDEVDQMFLRAASLLKKKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZEQ8</accession>
    <name>A0AA38ZEQ8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glucose-6-phosphate dehydrogenase NAD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_015751</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZEQ8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009570">
        <property type="term" value="C:chloroplast stroma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004345">
        <property type="term" value="F:glucose-6-phosphate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009051">
        <property type="term" value="P:pentose-phosphate shunt, oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001282">
        <property type="entry name" value="G6P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022674">
        <property type="entry name" value="G6P_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23429:SF13">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-DEHYDROGENASE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23429">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-DEHYDROGENASE G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00479">
        <property type="entry name" value="G6PD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="1" id="KW-0313">Glucose metabolism</keyword>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucose-6-phosphate dehydrogenase NAD-binding" evidence="4">
        <location>
            <begin position="101"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9687014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="318" mass="36171" checksum="1B9428772FBD4299" modified="2024-01-24" version="1">MATRINPCSSSSFKHDIHIPTRRFTIASSRTYHTSKWVSQISSRIHAKRHVELKASNGYPLNAVPLQDGNPLTEDHIAPQLKEGPFISGSESPESTLSITVVGASGDLAKKKIFPALFALFYEDWLPENFTVFGYARTKMTDEELRDMISRTLTCRIDKSANCGDKMDQFLKRCFYHSGQYSSEEHFAELDKKLKEKELPKSLTIHIKSTWAKVLIPFSGTALLSLTCMCFNIRFKLITTRTPWFGGMNLFRESMNIVRDGTNIFREVQTSLEKSGTNIVRQGTNFVRGGMNFFRESMNIVRDGTNIFREVQTSLEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZIP1</accession>
    <name>A0AA38ZIP1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Carboxypeptidase</fullName>
            <ecNumber evidence="9">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_014633</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the peptidase S10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="9"/>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9689083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZIP1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11320:FF:000003">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000579">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033124">
        <property type="entry name" value="Ser_caboxypep_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF32">
        <property type="entry name" value="SERINE CARBOXYPEPTIDASE-LIKE 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00560">
        <property type="entry name" value="CARBOXYPEPT_SER_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 9" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041489143" description="Carboxypeptidase" evidence="9">
        <location>
            <begin position="24"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9689083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="471" mass="53014" checksum="C0DBF1FDB334D57E" modified="2024-01-24" version="1">MALSPKWVFVVQILLTLINLNRATSSSSDPLVQQELDKVLQLPGQTFNISFAHYAGYVTVNEYTGRALFYWFIEAAEDPSSKPLVLWLNGGPGCSSIAYGQSEEIGPFHIKEDGKTLYLNPYSWNQAANILFLDSPVGVGFSYSNSSFDISSNGDIRTAKDSLKFLLEWFERFPQYKGRDFYVIGESYAGHYVPQLSQAIVRYNFATKAKSINLKGYMVGNALTDDFHDHLGLFQFMWSVGMISDQTYKLLNVFCDSQSFILSSELCDKIMDIAREEIGNIDLYSIFTPPCSVKIGFSNQLMKKLIMAGGISRKYDPCTQQHSVVYYNLPEVQQALHVYVDNATFKWATCSDEVSTTWKDSPKTVLDIYRELIHARLRIWIFSGDTDAVIPVTSTRYSINALKLPTVSPWRAWYDDGQVGGWTQDYAGLTFVTVRGAGHEVPLHKPKQAFTLFKAFLSGAPMPYMGQVSDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZLS2</accession>
    <name>A0AA38ZLS2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Cysteine-rich receptor-like protein kinase 25</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">PVL29_013577</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="23">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZLS2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009737">
        <property type="term" value="P:response to abscisic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23509">
        <property type="entry name" value="Gnk2-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000129">
        <property type="entry name" value="cysteine-rich receptor-like protein kinase 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000142">
        <property type="entry name" value="Cysteine-rich receptor-like protein kinase 10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.430.20:FF:000002">
        <property type="entry name" value="Cysteine-rich receptor-like protein kinase 10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000343">
        <property type="entry name" value="Cysteine-rich receptor-like protein kinase 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.430.20:FF:000003">
        <property type="entry name" value="Cysteine-rich RLK (RECEPTOR-like protein kinase) 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.430.20">
        <property type="entry name" value="Gnk2 domain, C-X8-C-X2-C motif"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002902">
        <property type="entry name" value="GNK2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038408">
        <property type="entry name" value="GNK2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002:SF1073">
        <property type="entry name" value="CYSTEINE-RICH RECEPTOR-LIKE PROTEIN KINASE 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002">
        <property type="entry name" value="RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD1-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01657">
        <property type="entry name" value="Stress-antifung"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51473">
        <property type="entry name" value="GNK2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="12 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 20" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041369781" description="Cysteine-rich receptor-like protein kinase 25" evidence="20">
        <location>
            <begin position="25"/>
            <end position="1185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="287"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="811"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="22">
        <location>
            <begin position="27"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="22">
        <location>
            <begin position="138"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="345"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="22">
        <location>
            <begin position="562"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="22">
        <location>
            <begin position="666"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="867"/>
            <end position="1140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="780"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="780"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="373"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="895"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAJ9691441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="25">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1185" mass="133253" checksum="F480B83859CF2E6C" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZLT5</accession>
    <name>A0AA38ZLT5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Small RNA 2'-O-methyltransferase</fullName>
            <ecNumber evidence="12">2.1.1.386</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Rotamase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PVL29_013407</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>small RNA 3'-end nucleotide + S-adenosyl-L-methionine = small RNA 3'-end 2'-O-methylnucleotide + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:37887"/>
            <dbReference type="Rhea" id="RHEA-COMP:10415"/>
            <dbReference type="Rhea" id="RHEA-COMP:10416"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74896"/>
            <dbReference type="ChEBI" id="CHEBI:74898"/>
            <dbReference type="EC" id="2.1.1.386"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyltransferase superfamily. HEN1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.386" evidence="12"/>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZLT5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090486">
        <property type="term" value="F:small RNA 2'-O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001510">
        <property type="term" value="P:RNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030422">
        <property type="term" value="P:siRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00048">
        <property type="entry name" value="DSRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000215">
        <property type="entry name" value="Hua enhancer1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000058">
        <property type="entry name" value="Small RNA 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056755">
        <property type="entry name" value="DSRM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026610">
        <property type="entry name" value="Hen1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040870">
        <property type="entry name" value="HEN1_dsRBD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040813">
        <property type="entry name" value="Hen1_Lam_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21404">
        <property type="entry name" value="HEN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21404:SF3">
        <property type="entry name" value="SMALL RNA 2'-O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17842">
        <property type="entry name" value="dsRBD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24995">
        <property type="entry name" value="DSRM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18441">
        <property type="entry name" value="Hen1_Lam_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21224">
        <property type="entry name" value="Hen1_LCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="10" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="dsRNA binding" evidence="17">
        <location>
            <begin position="23"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="Small RNA 2'-O-methyltransferase Hen1 La-motif C-terminal" evidence="16">
        <location>
            <begin position="225"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="HEN1 double-stranded RNA binding" evidence="15">
        <location>
            <begin position="360"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase FKBP-type" evidence="14">
        <location>
            <begin position="583"/>
            <end position="646"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00035025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF17842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF18441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF24995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ9691219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="950" mass="105498" checksum="0A3DDB5279419E1B" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZM58</accession>
    <name>A0AA38ZM58_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_013743</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZM58"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF78">
        <property type="entry name" value="OS12G0587133 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9691641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="562" mass="65030" checksum="10981B4A0C45601B" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZMI3</accession>
    <name>A0AA38ZMI3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GDSL esterase/lipase 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_011107</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9691831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZMI3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016298">
        <property type="term" value="F:lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044552">
        <property type="entry name" value="GLIP1-5/GLL25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45966:SF4">
        <property type="entry name" value="GDSL ESTERASE_LIPASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45966">
        <property type="entry name" value="GDSL-LIKE LIPASE/ACYLHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041324439" description="GDSL esterase/lipase 5" evidence="3">
        <location>
            <begin position="26"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9691831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="376" mass="42509" checksum="D96752358372785F" modified="2024-01-24" version="1">MARLISSLSCILIPTILLFPTSCLSKTQNPESQAAEASAALFIFGDSFFDSGNNNYINTTTLDQANFWPYGETYFKFPTGRFSDGRLISDFIAQYAKLPMIPPFLQPGVHQFYYGVNFASAGAGALVETFQGAVIDLKTQLKYYNEVVIWLRHKLGNFEAKMRLSRAVYLFSIGSNDYMSPFLTNSTILDSYSESEYVGMVIGNLTTVIKKIYSRGGRKFGFLNLPPLGCFPGLRVLKPDKNGSCLEKASMLAKLHNRALSKLLVKLENQLLGFKYSYYDLNSNLKQRMNRPAKYGFKEGKTACCGTGQFRGVFSCGGRRIVKEFQLCENPSEYVFWDSFHLTEKLYKQLADEMWSGTPYSDVRPYSLKNLFFPRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZPR2</accession>
    <name>A0AA38ZPR2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_011935</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9693021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZPR2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009791">
        <property type="term" value="P:post-embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000233">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase TDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000111">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051502">
        <property type="entry name" value="RLP_Defense_Trigger"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48062">
        <property type="entry name" value="RECEPTOR-LIKE PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48062:SF52">
        <property type="entry name" value="RECEPTOR-LIKE PROTEIN 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041252764" description="Leucine-rich repeat-containing N-terminal plant-type domain-containing protein" evidence="13">
        <location>
            <begin position="32"/>
            <end position="1119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1060"/>
            <end position="1083"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="14">
        <location>
            <begin position="36"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9693021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1119" mass="126730" checksum="35B512054954BC70" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZQ06</accession>
    <name>A0AA38ZQ06_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_011795</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZQ06"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9692869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="54" mass="5699" checksum="DDDD8A4F690C745E" modified="2024-01-24" version="1">MLSTGLLVDTNLGTSPPDTYRPPPAPIPYNVDLGNSQTPPATKESCVNKNDTTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZS86</accession>
    <name>A0AA38ZS86_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Carboxypeptidase</fullName>
            <ecNumber evidence="11">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_010036</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Probable carboxypeptidase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the peptidase S10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="11"/>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZS86"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11320:FF:000004">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000453">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033124">
        <property type="entry name" value="Ser_caboxypep_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF281">
        <property type="entry name" value="CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00560">
        <property type="entry name" value="CARBOXYPEPT_SER_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 11" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041487551" description="Carboxypeptidase" evidence="11">
        <location>
            <begin position="23"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9694341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="497" mass="55628" checksum="3BB86387E4243E90" modified="2024-01-24" version="1">MQLQPWIIMATISAFLIQICLTVESPSSADKIVSLPGQPQVGFQQFAGYITVDEKQQRHLFYYFVEAETDPASKPLVLWLNGGPGCSSIGAGAFCEHGPFKPSGEILVNNDYSWNKVANMLYLESPAGVGFSYSENTSFYAFVDDDMTARDNLIFLQRWFLKFPEYKNRDLFLTGESYAGHYVPQLAQLIVQSEVKFNLTGIAIGNPLLEFNTDFNSRAEYMWSHGLISDITYEAFTIICNYSQVRREIVMGSLSPACSGVKSQVSKELGKYIDSYDVTLDVCLPSVVSQSERLNQPRGTEKIDVCVEDETIKYLNRNDVQKALHAHLKGVSRWGVCSEVLKYEYRNLEIPTIHVVGAVLKSGIRVLVYSGDQDSVVPLTGTRTLVNGLAKDLGLNTTVHYRNWFQGRQVGGWTQVYGDKLSFATIRGASHEAPFSQPERSLVLFNTFLHGKPLPEATAGLMIKVIDMQFIIALDYVLPDFPIHLKFKQEINASSCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZTZ9</accession>
    <name>A0AA38ZTZ9_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_010032</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MOZART1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZTZ9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000931">
        <property type="term" value="C:gamma-tubulin ring complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031021">
        <property type="term" value="C:interphase microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033566">
        <property type="term" value="P:gamma-tubulin complex localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051415">
        <property type="term" value="P:microtubule nucleation by interphase microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090307">
        <property type="term" value="P:mitotic spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022214">
        <property type="entry name" value="MZT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28520">
        <property type="entry name" value="MITOTIC-SPINDLE ORGANIZING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28520:SF2">
        <property type="entry name" value="MITOTIC-SPINDLE ORGANIZING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12554">
        <property type="entry name" value="MOZART1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041282632" evidence="5">
        <location>
            <begin position="21"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9694334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="107" mass="12241" checksum="3E7DB7FE60F06A78" modified="2024-01-24" version="1">MEYTFKQRYFLLFLLRLVDWTKFFENRNPFQSICMDPEAAQTARESLELAFHMSNILDTGLDRHTLSVLITLCDLGLNPEALAAVVKELRREPCSSSPMPETPSSVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZVB6</accession>
    <name>A0AA38ZVB6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Signal recognition particle 9 kDa protein</fullName>
            <shortName evidence="9">SRP9</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_008292</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 9">Component of the signal recognition particle (SRP) complex, a ribonucleoprotein complex that mediates the cotranslational targeting of secretory and membrane proteins to the endoplasmic reticulum (ER). SRP9 together with SRP14 and the Alu portion of the SRP RNA, constitutes the elongation arrest domain of SRP. The complex of SRP9 and SRP14 is required for SRP RNA binding.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the SRP9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9695958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZVB6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005786">
        <property type="term" value="C:signal recognition particle, endoplasmic reticulum targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045900">
        <property type="term" value="P:negative regulation of translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.720.10:FF:000001">
        <property type="entry name" value="Signal recognition particle 9 kDa protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.720.10">
        <property type="entry name" value="Signal recognition particle alu RNA binding heterodimer, srp9/1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009018">
        <property type="entry name" value="Signal_recog_particle_SRP9/14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008832">
        <property type="entry name" value="SRP9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039914">
        <property type="entry name" value="SRP9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039432">
        <property type="entry name" value="SRP9_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12834">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 9 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12834:SF12">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 9 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05486">
        <property type="entry name" value="SRP9-21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017029">
        <property type="entry name" value="Signal_recog_particle_SRP9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54762">
        <property type="entry name" value="Signal recognition particle alu RNA binding heterodimer, SRP9/14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 9" id="KW-0733">Signal recognition particle</keyword>
    <feature type="domain" description="SRP9" evidence="10">
        <location>
            <begin position="5"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR017029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9695958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="103" mass="12036" checksum="5AD38124C94996F9" modified="2024-01-24" version="1">MVYIESWDEFVDRSVQLFRADPDSTRYVMKYRHCDGKLVLKVTDNRECLKFKTDQAQDAKKMEKLNNIFFTLMARGPEVDISEVTGKEAMEVQPAKKGRGRKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZWJ5</accession>
    <name>A0AA38ZWJ5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">STAS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_008396</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZWJ5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008271">
        <property type="term" value="F:secondary active sulfate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07042">
        <property type="entry name" value="STAS_SulP_like_sulfate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.750.24:FF:000002">
        <property type="entry name" value="Sulfate transporter 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="entry name" value="STAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018045">
        <property type="entry name" value="S04_transporter_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011547">
        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001902">
        <property type="entry name" value="SLC26A/SulP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00815">
        <property type="entry name" value="sulP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11814">
        <property type="entry name" value="SULFATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01740">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00916">
        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SpoIIaa-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01130">
        <property type="entry name" value="SLC26A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="128"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="157"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="265"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="318"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="359"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="393"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="418"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="455"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="domain" description="STAS" evidence="7">
        <location>
            <begin position="507"/>
            <end position="630"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9696132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="649" mass="70816" checksum="5A514EC1C7808AD0" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZYP2</accession>
    <name>A0AA38ZYP2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Smr domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_008751</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZYP2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000556">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000581">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000980">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:003743">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050872">
        <property type="entry name" value="PPR_P_subfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002625">
        <property type="entry name" value="Smr_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036063">
        <property type="entry name" value="Smr_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46128">
        <property type="entry name" value="MITOCHONDRIAL GROUP I INTRON SPLICING FACTOR CCM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46128:SF320">
        <property type="entry name" value="SMR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13812">
        <property type="entry name" value="PPR_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00463">
        <property type="entry name" value="SMR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160443">
        <property type="entry name" value="SMR domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50828">
        <property type="entry name" value="SMR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="221"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="256"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="292"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="327"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="362"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="397"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="432"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="467"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="502"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="636"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="domain" description="Smr" evidence="5">
        <location>
            <begin position="746"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="28"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="51"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9696689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="869" mass="96288" checksum="E435E42C7DFAD8DF" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZZC1</accession>
    <name>A0AA38ZZC1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Receptor-like serine/threonine-protein kinase</fullName>
            <ecNumber evidence="18">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">PVL29_007135</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="24">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="18"/>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9697864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZZC1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00028">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01098">
        <property type="entry name" value="PAN_AP_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000237">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.90.10.10:FF:000013">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase LECRK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000059">
        <property type="entry name" value="S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.90.10.10:FF:000024">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.10">
        <property type="entry name" value="Bulb-type lectin domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051343">
        <property type="entry name" value="G-type_lectin_kinases/EP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024171">
        <property type="entry name" value="SRK-like_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47976">
        <property type="entry name" value="G-TYPE LECTIN S-RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD2-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47976:SF108">
        <property type="entry name" value="G-TYPE LECTIN S-RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE LECRK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000641">
        <property type="entry name" value="SRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 18 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0430">Lectin</keyword>
    <keyword evidence="12 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 18 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 18" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 21" id="KW-0732">Signal</keyword>
    <keyword evidence="4 18" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041375409" description="Receptor-like serine/threonine-protein kinase" evidence="21">
        <location>
            <begin position="35"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="456"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="23">
        <location>
            <begin position="37"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="518"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="546"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRNR" id="PIRNR000641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KAJ9697864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="804" mass="89693" checksum="0D91744DB23005E7" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZZM0</accession>
    <name>A0AA38ZZM0_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_009154</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZZM0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9697243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="83" mass="9068" checksum="872982C8C45283EB" modified="2024-01-24" version="1">MMLMSSLGVVQAKPYLQAGAFEIVDESIKGNFDVESMRKAALIASRSVERDAAQRPVMAEVLAELKEAYSIQLSYLASCGHMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A143</accession>
    <name>A0AA39A143_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Receptor-like serine/threonine-protein kinase</fullName>
            <ecNumber evidence="19">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="ORF">PVL29_007885</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="26">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="26">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="19"/>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9699033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A143"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00028">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01098">
        <property type="entry name" value="PAN_AP_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.90.10.10:FF:000039">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase SD2-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000248">
        <property type="entry name" value="S-receptor-like kinase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000178">
        <property type="entry name" value="serine/threonine-protein kinase PBS1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.10">
        <property type="entry name" value="Bulb-type lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051343">
        <property type="entry name" value="G-type_lectin_kinases/EP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024171">
        <property type="entry name" value="SRK-like_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47976">
        <property type="entry name" value="G-TYPE LECTIN S-RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD2-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47976:SF30">
        <property type="entry name" value="RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000641">
        <property type="entry name" value="SRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 19 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 19" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0430">Lectin</keyword>
    <keyword evidence="13 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 19 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 19" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7 22" id="KW-0732">Signal</keyword>
    <keyword evidence="5 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041225029" description="Receptor-like serine/threonine-protein kinase" evidence="22">
        <location>
            <begin position="30"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="467"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="24">
        <location>
            <begin position="58"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="25">
        <location>
            <begin position="356"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="524"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="552"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRNR" id="PIRNR000641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EMBL" id="KAJ9699033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="840" mass="92633" checksum="A24D6B115356848D" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A1K8</accession>
    <name>A0AA39A1K8_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_008054</name>
        <name evidence="4" type="ORF">PVL29_008056</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9699270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9699272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A1K8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42861">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="55"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="93"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9699270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9699272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="119" mass="13991" checksum="8EA73B32D601FAB7" modified="2024-01-24" version="1">MSHGNHRHYGYCFSKWWWPSSRLARLYWYHCFSVGRTPQSVSLKKTMLLFYFQKVLIVISNFCVCSIAVGMIIKIIVMCPIQHYKYRDGIDNLLVLLIGGIPMQMPTIPFILYHVPFQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A310</accession>
    <name>A0AA39A310_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cullin-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_005727</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the cullin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A310"/>
    <dbReference type="SMR" id="A0AA39A310"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031461">
        <property type="term" value="C:cullin-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009867">
        <property type="term" value="P:jasmonic acid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000503">
        <property type="entry name" value="Cullin-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000013">
        <property type="entry name" value="Cullin-1 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.130:FF:000005">
        <property type="entry name" value="Cullin-1 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000020">
        <property type="entry name" value="Cullin-1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000021">
        <property type="entry name" value="Cullin-1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000025">
        <property type="entry name" value="Cullin-1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1310.10">
        <property type="entry name" value="Cullin Repeats"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.130">
        <property type="entry name" value="Cullin, Chain C, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045093">
        <property type="entry name" value="Cullin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059120">
        <property type="entry name" value="Cullin-like_AB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016157">
        <property type="entry name" value="Cullin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016158">
        <property type="entry name" value="Cullin_homology"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036317">
        <property type="entry name" value="Cullin_homology_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001373">
        <property type="entry name" value="Cullin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019559">
        <property type="entry name" value="Cullin_neddylation_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11932">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00888">
        <property type="entry name" value="Cullin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26557">
        <property type="entry name" value="Cullin_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10557">
        <property type="entry name" value="Cullin_Nedd8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00182">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00884">
        <property type="entry name" value="Cullin_Nedd8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75632">
        <property type="entry name" value="Cullin homology domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01256">
        <property type="entry name" value="CULLIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50069">
        <property type="entry name" value="CULLIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Cullin family profile" evidence="8">
        <location>
            <begin position="389"/>
            <end position="619"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00069612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9700035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="744" mass="86889" checksum="8A8FC16B13D00362" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A3E2</accession>
    <name>A0AA39A3E2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ATP citrate synthase</fullName>
            <ecNumber evidence="3">2.3.3.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_005594</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Heterooctamer of 4 alpha and 4 beta chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.8" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9699800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A3E2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003878">
        <property type="term" value="F:ATP citrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006085">
        <property type="term" value="P:acetyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06100">
        <property type="entry name" value="CCL_ACL-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000136">
        <property type="entry name" value="ATP-citrate synthase beta chain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.261:FF:000003">
        <property type="entry name" value="ATP-citrate synthase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.230.10">
        <property type="entry name" value="Cytochrome P450-Terp, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017440">
        <property type="entry name" value="Cit_synth/succinyl-CoA_lig_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016143">
        <property type="entry name" value="Citrate_synth-like_sm_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002020">
        <property type="entry name" value="Citrate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036969">
        <property type="entry name" value="Citrate_synthase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033847">
        <property type="entry name" value="Citrt_syn/SCS-alpha_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017866">
        <property type="entry name" value="Succ-CoA_synthase_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="SUCC_ACL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23118">
        <property type="entry name" value="ATP-CITRATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23118:SF42">
        <property type="entry name" value="ATP-CITRATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00285">
        <property type="entry name" value="Citrate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48256">
        <property type="entry name" value="Citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01216">
        <property type="entry name" value="SUCCINYL_COA_LIG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00399">
        <property type="entry name" value="SUCCINYL_COA_LIG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01217">
        <property type="entry name" value="SUCCINYL_COA_LIG_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ATP-citrate synthase/succinyl-CoA ligase C-terminal" evidence="12">
        <location>
            <begin position="173"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9699800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="608" mass="66079" checksum="D94E1C27519DFF0D" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A4C9</accession>
    <name>A0AA39A4C9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">At4g14310 8-bladed propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_006185</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A4C9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045289">
        <property type="entry name" value="At4g14310-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057442">
        <property type="entry name" value="Beta-prop_At4g14310"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35492">
        <property type="entry name" value="TRANSDUCIN/WD40 REPEAT-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35492:SF1">
        <property type="entry name" value="TRANSDUCIN_WD40 REPEAT-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25465">
        <property type="entry name" value="Beta-prop_At4g14310"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="At4g14310 8-bladed propeller" evidence="2">
        <location>
            <begin position="724"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="258"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="322"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="407"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="89"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="418"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9700743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1012" mass="110464" checksum="DC184C3077F0AB00" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A641</accession>
    <name>A0AA39A641_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_006774</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A641"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14798">
        <property type="entry name" value="RX-CC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038005">
        <property type="entry name" value="RX-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF955">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="5">
        <location>
            <begin position="146"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="4">
        <location>
            <begin position="311"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="6">
        <location>
            <begin position="548"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="7">
        <location>
            <begin position="686"/>
            <end position="986"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9701551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1014" mass="116183" checksum="BD6587028300E0DA" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2025-10-08" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A6M9</accession>
    <name>A0AA39A6M9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phosphoinositide phosphatase SAC9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_006899</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057554">
        <property type="entry name" value="SAC9_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057557">
        <property type="entry name" value="SAC9_C8D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057555">
        <property type="entry name" value="SAC9_GBDL_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057553">
        <property type="entry name" value="SAC9_GBDL_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002013">
        <property type="entry name" value="SAC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46817">
        <property type="entry name" value="PHOSPHOINOSITIDE PHOSPHATASE SAC9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46817:SF1">
        <property type="entry name" value="SAC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24765">
        <property type="entry name" value="SAC9_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24791">
        <property type="entry name" value="SAC9_C8D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24790">
        <property type="entry name" value="SAC9_GBDL_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24789">
        <property type="entry name" value="SAC9_GBDL_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02383">
        <property type="entry name" value="Syja_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50275">
        <property type="entry name" value="SAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAC" evidence="2">
        <location>
            <begin position="151"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="1">
        <location>
            <begin position="512"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9701719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1611" mass="178119" checksum="B9ADB9E2E87BBBC1" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A7Z9</accession>
    <name>A0AA39A7Z9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Trafficking protein particle complex II-specific subunit 120 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_004412</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A7Z9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058565">
        <property type="entry name" value="Ig_TRAPPC9_Trs120_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058567">
        <property type="entry name" value="Ig_TRAPPC9_Trs120_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058568">
        <property type="entry name" value="Ig_TRAPPC9_Trs120_4th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058564">
        <property type="entry name" value="TPR_TRAPPC9_Trs120"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013935">
        <property type="entry name" value="Trs120_TRAPPC9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058563">
        <property type="entry name" value="Trs120_TRAPPC9_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21512">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21512:SF5">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26254">
        <property type="entry name" value="Ig_TRAPPC9-Trs120_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26282">
        <property type="entry name" value="Ig_TRAPPC9-Trs120_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26283">
        <property type="entry name" value="Ig_TRAPPC9-Trs120_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26251">
        <property type="entry name" value="TPR_TRAPPC9-Trs120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08626">
        <property type="entry name" value="TRAPPC9-Trs120"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Trs120/TRAPPC9 N-terminal" evidence="3">
        <location>
            <begin position="9"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="Trs120/TRAPPC9 N-terminal" evidence="3">
        <location>
            <begin position="202"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="Trs120/TRAPPC9 TPR region" evidence="4">
        <location>
            <begin position="308"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="domain" description="Trs120/TRAPPC9 first Ig-like" evidence="5">
        <location>
            <begin position="523"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="domain" description="Trs120/TRAPPC9 third Ig-like" evidence="6">
        <location>
            <begin position="860"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="domain" description="Trs120/TRAPPC9 fourth Ig-like" evidence="7">
        <location>
            <begin position="1046"/>
            <end position="1176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF26251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF26254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF26282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF26283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9702666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1180" mass="127990" checksum="A01F0305F73312D0" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A834</accession>
    <name>A0AA39A834_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ApaG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_006813</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A834"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1470">
        <property type="entry name" value="ApaG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007474">
        <property type="entry name" value="ApaG_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036767">
        <property type="entry name" value="ApaG_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037883">
        <property type="entry name" value="Knr4/Smi1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47463">
        <property type="entry name" value="F-BOX PROTEIN SKIP16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47463:SF2">
        <property type="entry name" value="F-BOX PROTEIN SKIP16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04379">
        <property type="entry name" value="DUF525"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110069">
        <property type="entry name" value="ApaG-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160631">
        <property type="entry name" value="SMI1/KNR4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51087">
        <property type="entry name" value="APAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="ApaG" evidence="3">
        <location>
            <begin position="304"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9701594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="445" mass="49340" checksum="B09D491AD2DF0E77" modified="2024-01-24" version="1">MEMGLEDLGDLAINAIISKLDAKDTVAVSCVSKKLRVSASEESLWLNHCSQDLGLSSPIDPLGKPAPSFKETYQLWRESFGMYPWPLVKRVKRCWDRLKNWLSANFPEAGATLRKGATEVEIEALENILKVKLPLPTRLLYRFCDGQELTEEDVGGTALGSSLGIIGGYCFYDHLVNVCLLPLRQVILETKEITDQLGFSTTSKYVIVAASSTYIGKFFFLNCTTGQLYVGTRNLLSAGEMIPCVPNALISPMHDLNTGQQQDAMLLWLEEHVRRLQNGIIKLRKEGMIRSINLFPEEPPLCSTAVTNGVQVRASAVFIPEGCNLLDESHKYLFAYSIRMRLLPEGCIVNGTSFGSCQLNLRHWIIRANDHVVSDVNGEAVIGKYPLLYPGGEEFVYESCTFLSSSQGSIEGGFTFVPGRLADPKGSAFEVEVGRFPLQCPDYIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A9X8</accession>
    <name>A0AA39A9X8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Alpha/beta hydrolase fold-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_004347</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDXG' lipolytic enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A9X8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050466">
        <property type="entry name" value="Carboxylest/Gibb_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23024">
        <property type="entry name" value="ARYLACETAMIDE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23024:SF635">
        <property type="entry name" value="OS07G0162700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="2">
        <location>
            <begin position="70"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9702568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="310" mass="34674" checksum="183E3185990E80C1" modified="2024-01-24" version="1">MLLVADFPGFIQVFSDGSVKRYERETAPASNDSSSNGYKSKDVIISSTKPISARIFLPDTLDSSSHLPVLVYFHGGGFCAVSTTWSGHHTFLGDFAVASQSIVLSVDYRLAPENRLPIAYDDCYSSLEWLSCLACSDPWLERADLSRVFLSGESAGGNIIHYVALRTIQEQSCDQVKIKGLLPIHPFFGSQERTEKERASGEAENLAKTDLLWKLSLPEGSNRDHPWCNFEKAELSRAEWSSYPPVVVYVAGLDFLKERGVMYAAFLKKKGVEVKLVEAEGEDHMYHVLHPESKATRLLQKQMSEFIHNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AAE0</accession>
    <name>A0AA39AAE0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TFIIS N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_005292</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AAE0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00183">
        <property type="entry name" value="TFIIS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.10">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044790">
        <property type="entry name" value="MD26C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003617">
        <property type="entry name" value="TFIIS/CRSP70_N_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035441">
        <property type="entry name" value="TFIIS/LEDGF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017923">
        <property type="entry name" value="TFIIS_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47210">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 26C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47210:SF1">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 26C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08711">
        <property type="entry name" value="Med26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00509">
        <property type="entry name" value="TFS2N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47676">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51319">
        <property type="entry name" value="TFIIS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="305"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="TFIIS N-terminal" evidence="6">
        <location>
            <begin position="106"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="64"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="194"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="223"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="235"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="255"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9703950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="361" mass="41049" checksum="F0FDD3A7CA39E1AF" modified="2024-01-24" version="1">MDRDEFRLILRNSGVDVWEFINTAISVAYSDYGDELRHRRDGIVERMYMSSRCGSCNLRYQHSGLDKGCDEEKEDEKDDEKDKSPRTPQSINRSGSEDRYGGLLDEEEARILDIKEHLEAPDQPDDSIVDLLQTLADMDITFKALKETDIGRHVNRLRKHPSNDVRRLVKHLVRKWKDLVDEWVKSNPAGEFTSSALMAEGDSPPQKIPRTGHHQVLDFGYSPNPQNGSSGSDKNNAEPEPKVKAIPRKEVPTRPAQSAPVSSSFPPPNKQRDTAIDPDKFASARKRLHENYQEAQNGLILSSKLWIHNVLLLVLELMVCVGLFVEAKKQRTIQVMDIHDIPKPKNTFFAKPKVGSQGRHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AAH9</accession>
    <name>A0AA39AAH9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Carbamoyl phosphate synthase small chain, chloroplastic</fullName>
            <ecNumber evidence="4">6.3.5.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Carbamoyl phosphate synthetase glutamine chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PVL29_005320</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Small subunit of the arginine-specific carbamoyl phosphate synthase (CPSase). CPSase catalyzes the formation of carbamoyl phosphate from the ammonia moiety of glutamine, carbonate, and phosphate donated by ATP, the first step of the arginine biosynthetic pathway. The small subunit (glutamine amidotransferase) binds and cleaves glutamine to supply the large subunit with the substrate ammonia.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Heterodimer composed of 2 chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CarA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.5" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AAH9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005951">
        <property type="term" value="C:carbamoyl-phosphate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004088">
        <property type="term" value="F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006221">
        <property type="term" value="P:pyrimidine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01744">
        <property type="entry name" value="GATase1_CPSase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.20:FF:000001">
        <property type="entry name" value="Carbamoyl-phosphate synthase small chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000034">
        <property type="entry name" value="carbamoyl-phosphate synthase small chain, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.20">
        <property type="entry name" value="Carbamoyl-phosphate synthase small subunit, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01209">
        <property type="entry name" value="CPSase_S_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006274">
        <property type="entry name" value="CarbamoylP_synth_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002474">
        <property type="entry name" value="CarbamoylP_synth_ssu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036480">
        <property type="entry name" value="CarbP_synth_ssu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035686">
        <property type="entry name" value="CPSase_GATase1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01368">
        <property type="entry name" value="CPSaseIIsmall"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009475">
        <property type="entry name" value="PRK12838.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405:SF4">
        <property type="entry name" value="CARBAMOYL-PHOSPHATE SYNTHASE ARGININE-SPECIFIC SMALL CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405">
        <property type="entry name" value="CARBAMOYLTRANSFERASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00988">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00097">
        <property type="entry name" value="ANTSNTHASEII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00099">
        <property type="entry name" value="CPSGATASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00096">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01097">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52021">
        <property type="entry name" value="Carbamoyl phosphate synthetase, small subunit N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="6" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carbamoyl-phosphate synthase small subunit N-terminal" evidence="17">
        <location>
            <begin position="52"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00043861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00074572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00083899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM01097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ9703986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="426" mass="46245" checksum="032D68CA0306F111" modified="2024-01-24" version="1">MAIRCTGFGVKNWFASNPGNYASINARIFTIKCSSGATATAVGLGERPWKTSDARLVLEDGSVWKAKSFGASGTQVGEVVFNTSLTGYQEILTDPSYAGQFVLMTNPHIGNTGVNFDDEESSQCFLAGLVIRSLSIGTSNWRCADTLGDYLAERNIMGIYDVDTRAITRRLRQDGSLIGVLSTEDSKTDEELLKMSCSWDIVGVDLISGVSCNSPYEWVDKTGSEWDFNSNERAGETFRVIAYDFGIKHNILRRLASYGCKITVVPSTWPASETLKMKPDGVLFSNGPGDPSAVPYAVETVKEILGKVPVFGICMGHQLLGQALGGKTFKMKFGHHGGNHPVRNLRSGRVEISAQNHNYAVDPASLPEGVEVTHVNLNDGSCAGLAYPALNIMSLQYHPEASPGPHDSDPAFREFIQLMKQVKQNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ADR6</accession>
    <name>A0AA39ADR6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">glycerate dehydrogenase</fullName>
            <ecNumber evidence="10">1.1.1.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_002837</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(R)-glycerate + NAD(+) = 3-hydroxypyruvate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17905"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16659"/>
            <dbReference type="ChEBI" id="CHEBI:17180"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.29"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="9">Photosynthesis; photorespiration; 3-phospho-D-glycerate from glycine: step 3/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.29" evidence="10"/>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ADR6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030267">
        <property type="term" value="F:glyoxylate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008465">
        <property type="term" value="F:hydroxypyruvate reductase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009854">
        <property type="term" value="P:oxidative photosynthetic carbon pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05301">
        <property type="entry name" value="GDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000207">
        <property type="entry name" value="Glycerate dehydrogenase HPR, peroxisomal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050223">
        <property type="entry name" value="D-isomer_2-hydroxyacid_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029752">
        <property type="entry name" value="D-isomer_DH_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996">
        <property type="entry name" value="2-HYDROXYACID DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996:SF257">
        <property type="entry name" value="GLYOXYLATE REDUCTASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00065">
        <property type="entry name" value="D_2_HYDROXYACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0323">Glycolate pathway</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="4 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="7" id="KW-0601">Photorespiration</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase catalytic" evidence="12">
        <location>
            <begin position="58"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding" evidence="13">
        <location>
            <begin position="157"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00052847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00060654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00066607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9704459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="437" mass="48312" checksum="401F636BA69CCD59" modified="2024-01-24" version="1">MHINKTEHLKFERLRFFLPTPELLLASMAKPVSIEVWNPSGKYRVVSTKPMPGTRWIDLLVQQDCRVEICTQKKTILSVEDIIALIGDKCDGVIGQLTEDWGETLFSALSRAGGRAFSNMAVGYNNVDVNAANKYGVAVGNTPGVLTETTAELAASLSLSAARRIVEADEFMRAGLYDGWLPHLFVGNLLRGQTVGVIGAGRIGSAYARMMVEGFKMNLIYYDLYQATRLEKFVTAYGQFLKASGEQPVTWKRAASMDEVLREADVISLHPILDKTTYHLVNKERLSMMKKEAILINCSRGPVIDEVALVAHLKENPMFRVGLDVFEVTKQNSPFTFPSSFIIQLSIHFFQDEPYMKPGLAEMKNAIVVPHIASASKWTREGMATLAALNVLGKIKGYPVWHDPNKVEPFLNENSLPPAASPSIVNAKALGLPVSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AFN9</accession>
    <name>A0AA39AFN9_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_001644</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AFN9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36048">
        <property type="entry name" value="RIBOSOME MATURATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36048:SF1">
        <property type="entry name" value="RIBOSOME MATURATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="136"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="184"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="137"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="187"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9706190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="215" mass="24817" checksum="A10605F41755017F" modified="2024-01-24" version="1">MAAKPLTSEAIALTEKKMDMTLDDIIKMSKSTTVKDKKPRTPNKSQKFLSGIAQDKSAKMRRFMDSRSSLRQGVLAQRRSNFQANQFPLANEVARKAAAVPIRNRAFNRNRMPNWNRPRVGALHRNVANGGFTVKYQQQQHKQQQQHQHQQQQQQQQHQHQQVKPVPKQRPQTLDSLFADMKEQRMRVSSRQNNAGRQNGFGVQRLPRGRGRFGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AFR5</accession>
    <name>A0AA39AFR5_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_001951</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AFR5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9706727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="83" mass="8932" checksum="7C0BC255247D33BB" modified="2024-01-24" version="1">MVLSDSGVPAVLDAEVVNIFCFLIAAAILVAVMGFTLIWCAVCLPSPIPRPALSARTPLLRYLGVATIRPFRFRAGTEDPFLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AG98</accession>
    <name>A0AA39AG98_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">FAD-binding PCMH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_003799</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oxygen-dependent FAD-linked oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AG98"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.43.10:FF:000004">
        <property type="entry name" value="Berberine bridge enzyme-like 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.462.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="entry name" value="Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012951">
        <property type="entry name" value="BBE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32448">
        <property type="entry name" value="OS08G0158400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08031">
        <property type="entry name" value="BBE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041339110" description="FAD-binding PCMH-type domain-containing protein" evidence="8">
        <location>
            <begin position="26"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="9">
        <location>
            <begin position="75"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9705868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="472" mass="53028" checksum="71FA03BB117AE8F9" modified="2024-01-24" version="1">MEMSRSSFGLLLLLGILFVSSYAAADSVYDTFVQCLSSHSAPSDQASSIVYAQTNSSFIKVLQSYIRNQQFNTSSTPKPLIIVTPSDESQVQAAIICPRNIGIQLRIRSGGHDYDGLSYISDVPFFILDMFNLRSISVNINDKTAWVLAGATLGELSYRIWEKSKVHGFPVGICPTLGVGGHFSGAGYGYMLRRYGLSVYHVVDAQIVNVNRSILDRKSMGEDLFWAIRGGGGASFGVILSYKIKLVRVPEIVTVFRVEKTLAQNATGIVYQWQHITDKIDNDIFMRLLLLPITVKSDKGSAKAEKIPKTIRVTLVSLFLGDTRPVLFHWIESVLYWANFDNGTSRKSDYVQKPISKDGLEGLWKTMIELGKPGMVFNAYGGRMRNPLPSPRWEHIQDPVLDKKYLNLIRELYSYITPFVSNSPRGTDSYEEGKVYGVKYFMNNFDRLVKVKTAVDPQNFFRYEQSIPPLPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AHE8</accession>
    <name>A0AA39AHE8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF4283 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_002641</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AHE8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="180"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="229"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="296"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="601"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="256"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="334"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="383"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="450"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="614"/>
            <end position="626"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9707688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="667" mass="73510" checksum="472290B0B254CFE6" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AI15</accession>
    <name>A0AA39AI15_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">inositol-phosphate phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_000156</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Polyol metabolism; myo-inositol biosynthesis; myo-inositol from D-glucose 6-phosphate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the inositol monophosphatase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.25" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9707957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AI15"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008934">
        <property type="term" value="F:inositol monophosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006020">
        <property type="term" value="P:inositol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01639">
        <property type="entry name" value="IMPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.540.10:FF:000003">
        <property type="entry name" value="Inositol-1-monophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.80:FF:000002">
        <property type="entry name" value="Inositol-1-monophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.10">
        <property type="entry name" value="Fructose-1,6-Bisphosphatase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033942">
        <property type="entry name" value="IMPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020583">
        <property type="entry name" value="Inositol_monoP_metal-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020550">
        <property type="entry name" value="Inositol_monophosphatase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20854">
        <property type="entry name" value="INOSITOL MONOPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20854:SF17">
        <property type="entry name" value="PHOSPHATASE IMPL1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00377">
        <property type="entry name" value="IMPHPHTASES"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56655">
        <property type="entry name" value="Carbohydrate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00629">
        <property type="entry name" value="IMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00630">
        <property type="entry name" value="IMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR600760-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9707957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="402" mass="44050" checksum="976834732664A433" modified="2024-01-24" version="1">MGRSLLSSTVIPVRFPQIPKSIPPLDRSIHPIPPTFALNSQRIRGFSIPTKTFCTKAVLSESPNPKRYPKMGSDSVGPIPPSQLIQVVEHAANTGAQVVMEAVNKPRNVTYKGLTDLVTDTDKMSETAILEVVRKNFGDHLILGEEGGLIGDTSSDYLWCIDPLDGTTNFAHCYPSFAVSVGVLFRGKPAAAAVVEFVGGPMCWNTRIFSATTGNKVLSNELYLMHILYSNYEIKVGNVILTVYLFLGGGAFCNGQKIHASQTDLVEQSLLVTGFGYEHDDAWATNIDLFKEFTDVSRGVRRLGAAAVDMCHVALGIVEAYWEYRLKPWDMAAGVLIVEEAGGTVTCMDGGKFCVFDRSVLVSNGVLHSKLLERIGPATEKLKKKGIDFSLWYKPENYNTDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AIL0</accession>
    <name>A0AA39AIL0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Polygalacturonase QRT3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_000116</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9707884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AIL0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004650">
        <property type="term" value="F:polygalacturonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000046">
        <property type="entry name" value="Polygalacturonase QRT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039279">
        <property type="entry name" value="QRT3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33928:SF2">
        <property type="entry name" value="PECTATE LYASE SUPERFAMILY PROTEIN DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33928">
        <property type="entry name" value="POLYGALACTURONASE QRT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041237594" description="Polygalacturonase QRT3" evidence="1">
        <location>
            <begin position="23"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9707884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="472" mass="50909" checksum="FA21D4251807D692" modified="2024-01-24" version="1">MMKASWAICILLLLSLHKPTFSSFHQKLSEFQSRLQNTVPPPFPSLDGSQNGRVFYPIAYGADPTGVNESSDAILSALTDAFSIRNGVEMLPGINDLGGVVIDLQGGNYKISKPIRFPAGGGNVLVCQGTLRASDTFPDDEHLIELWSPNSQKLNTSSINPDDFSIIKAQNNMIYYEDITFRDILFDSNNSGGGLFMIDTARIRINNCFFLHFTTQGILVQKGHENFISSSFLGQHSTVGGDRGERDFSGTAIDLGGNDNAVTDVAIFSAAIGVLLRGQANIITGVHCYNKATGFGGVGILVKAGGSLTRISNCYLDFNAIVMEDPSQVHVTNGFFLGDGNVVLKSVQGHVRGLNIVDNMFNGNPSNMKAIVELDGQFTDIDQVVVDRNNAIGMSLKSTVARLTIPGNGTHWVADFSSLLLFPNRINHFQYSVYGQGGSGFVADSVINVSNNMLVVESQNPIQGLVSIVVEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AK43</accession>
    <name>A0AA39AK43_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_000606</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AK43"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044550">
        <property type="term" value="P:secondary metabolite biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19124">
        <property type="entry name" value="AKR_AKR4A_4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000014">
        <property type="entry name" value="NAD(P)-linked oxidoreductase superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044497">
        <property type="entry name" value="AKR4A/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11732">
        <property type="entry name" value="ALDO/KETO REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00798">
        <property type="entry name" value="ALDOKETO_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00063">
        <property type="entry name" value="ALDOKETO_REDUCTASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="5">
        <location>
            <begin position="19"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="4">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9708664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="316" mass="35393" checksum="AE9E4BBE1F43D3E7" modified="2024-01-24" version="1">MAKSPEVSLGACGLTMPVLGMGTSSYPPADPETAKLAIIEAIKAGYRHFDTAFAYGSEQPLGQAIAEALRLGLIKSRDDLFITSKLWSSYTDRDQVVPAIKMSLRNLQLDYLDMYLIHLPLKLSQEIRKTPVPKEILMPLDIKSVWEGMEECQRLGLTKAIGVSNFSPRMLEETLAIAEIPPAVNQVEMNPLWQQKKLREYCNAKGIHITAYSPLGANGTKWGDNRIVECDVLEDIAKARGKTSAQVSLRWVYEQGVSIVAKSFNKERMKQNLEIFDWSLTEEELERISQLPQRKGFLISSIIGAHDLTLQLDDEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AL68</accession>
    <name>A0AA39AL68_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">Pectate lyase</fullName>
            <ecNumber evidence="4 10">4.2.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_000932</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>Eliminative cleavage of (1-&gt;4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.</text>
            <dbReference type="EC" id="4.2.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="10">Binds 1 Ca(2+) ion. Required for its activity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 2/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the polysaccharide lyase 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.2.2" evidence="4 10"/>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AL68"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030570">
        <property type="term" value="F:pectate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018082">
        <property type="entry name" value="AmbAllergen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002022">
        <property type="entry name" value="Pec_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007524">
        <property type="entry name" value="Pec_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045032">
        <property type="entry name" value="PEL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31683">
        <property type="entry name" value="PECTATE LYASE 18-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31683:SF181">
        <property type="entry name" value="PECTATE LYASE 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04431">
        <property type="entry name" value="Pec_lyase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00544">
        <property type="entry name" value="Pectate_lyase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00807">
        <property type="entry name" value="AMBALLERGEN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00656">
        <property type="entry name" value="Amb_all"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 10" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041485346" description="Pectate lyase" evidence="10">
        <location>
            <begin position="27"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="domain" description="Pectate lyase" evidence="11">
        <location>
            <begin position="164"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9709219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="443" mass="49896" checksum="863BA8C5CE657C97" modified="2024-01-24" version="1">MAAPKSNSLLLLFVVMFMVVVPTIRANIAEYDDYWKAREKEAKTVAIDAFDPSPEDVNDDFNKNVEKSIFGKNGTRRNLKVNKGPCKATNPIDRCWRCRRNWAMDRRRLADCVLGFGRRTVGGKHGRLYIVTDPSDDDMLNPKPGTLRYAVIQPQPLWIVFGRSMIIRLKQELIVTSDKTIDGRGVNVHIAYGAGITIQFVRNVIIHGLHIHDIVSRPGGLIRDSVNHFGLRTRSDGDAISIFGSSHVWIDHNSLSECEDGLIDAIQGSTAITISNNLFTKHNETMLLGASDGYSGDIIMQVTVAFNHFGRGLVQRMPRCRWGFFHVVNNDYTHWNMYAVGGSAHPTIVSQGNRYVAAPLMDPKHDAKEVTKRDHATKAEWSNWTWRSEGDLMVNGAFFVESGVPFKKKPFSRYDMIKAKPGKFVPRLTRYSGALNCWRTSPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ALD2</accession>
    <name>A0AA39ALD2_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_000880</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ALD2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9709130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="137" mass="15407" checksum="BB918A41AC683BA6" modified="2024-01-24" version="1">MTNKARYKRESLCEKSMDLIMSIIKLSSLSLASKSFRTARLPAKRNRVPVTGAVTVSIPHQLPASQALQEPESGSKSYSYLIEIERKSPSYMIPEIESVDDRASDYIRGFHAKNRHDSSTASKQLSYILSPPPPFYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ALP4</accession>
    <name>A0AA39ALP4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Apyrase 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_000999</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GDA1/CD39 NTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ALP4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017110">
        <property type="term" value="F:nucleoside diphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009134">
        <property type="term" value="P:nucleoside diphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd24042">
        <property type="entry name" value="ASKHA_NBD_AtAPY3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.150">
        <property type="entry name" value="Exopolyphosphatase. Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000407">
        <property type="entry name" value="GDA1_CD39_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11782">
        <property type="entry name" value="ADENOSINE/GUANOSINE DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11782:SF3">
        <property type="entry name" value="APYRASE 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01150">
        <property type="entry name" value="GDA1_CD39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="483"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="195"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="225"/>
            <end position="229"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR600407-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR600407-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9709310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="529" mass="58752" checksum="BA6CFAA04FFC2E68" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ANQ3</accession>
    <name>A0AA39ANQ3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TOD1/MUCI70 glycosyltransferase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_000972</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ANQ3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006852">
        <property type="entry name" value="TOD1_MUCI70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048354">
        <property type="entry name" value="TOD1_MUCI70_glycTrfase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12956">
        <property type="entry name" value="ALKALINE CERAMIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12956:SF27">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04765">
        <property type="entry name" value="TOD1_MUCI70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="TOD1/MUCI70 glycosyltransferase-like" evidence="2">
        <location>
            <begin position="137"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9709274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="464" mass="54367" checksum="06C2CF8D1CAC065B" modified="2024-01-24" version="1">MMALFRHSAELLPERKDNVVIEFKSLNRNEHRIRRGRKFGRITKQRFSVWLTVLAVLFLIYLIFFGIKMLFHGKMEGGISTSIQVQEEIMGVDELDVSRQAKSKHRKKYPSCEVGLSDSVDHLVEPKDFVNFTQFSLDYIDKEEKPFGKSPSRFGGQQSLEEREKSFYARNQTLHCGFVKGPEGSPSTGFDLDANDKTYMNTCKVVVSSCIFGNSDFLRRPTRKRISEYSKKNVCFVMFVDEQTLSKLSLEGNFPDDGGYIGLWKIVVVRNLPYKDMRRTGKVPKFLSHRLFPSSMYSIWLDSKMRLNTDPMLILEYFLWRMRSEYAISNHYDRHCVWEEVLQNKRLNKYNHSAIDEQFNFYQSDGLTKFDPSDPNNPIPSYVPEGSFIVRAHTPMSNLFSCLWFNEVDRFTSRDQLSFAYTYLKLRRMNPDRPFFLNMFKDCERRALAKLFRHKAVPSPPPAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D5J0</accession>
    <name>A0AA39D5J0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fungal lipase-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_026956</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D5J0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00519">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002921">
        <property type="entry name" value="Fungal_lipase-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044819">
        <property type="entry name" value="OBL-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46086">
        <property type="entry name" value="ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46086:SF17">
        <property type="entry name" value="ALPHA_BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01764">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fungal lipase-type" evidence="2">
        <location>
            <begin position="232"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9670735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="520" mass="59560" checksum="848CB75210974BAD" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D6G4</accession>
    <name>A0AA39D6G4_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_026225</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D6G4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9672881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="81" mass="8757" checksum="F1A3BC1AAE4F2AA9" modified="2024-01-24" version="1">MEGLTFHSIESFCRGTALYTSQTQPIVNAGLECGRGMGIEDYSDWEVGDIVEAFSKRQKKPTPEEASASMTAALKVAGIEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D6Z1</accession>
    <name>A0AA39D6Z1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_025288</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D6Z1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015369">
        <property type="term" value="F:calcium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004713">
        <property type="entry name" value="CaH_exchang"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31503:SF79">
        <property type="entry name" value="CALCIUM-BINDING EF-HAND PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31503">
        <property type="entry name" value="VACUOLAR CALCIUM ION TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0050">Antiport</keyword>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041375413" description="EF-hand domain-containing protein" evidence="10">
        <location>
            <begin position="26"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="147"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="245"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="427"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="458"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="499"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="527"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="553"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="11">
        <location>
            <begin position="307"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="11">
        <location>
            <begin position="347"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9671512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="580" mass="64684" checksum="3565B22C479E3BF9" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D8G3</accession>
    <name>A0AA39D8G3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">PGG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_023496</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D8G3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000477">
        <property type="entry name" value="Ankyrin repeat family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026961">
        <property type="entry name" value="PGG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24186:SF37">
        <property type="entry name" value="PGG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24186">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13962">
        <property type="entry name" value="PGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="323"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="384"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="411"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="457"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="53"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="87"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="121"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="155"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="PGG" evidence="9">
        <location>
            <begin position="316"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9673975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="490" mass="54433" checksum="BC6B24811F5D7B99" modified="2024-01-24" version="1">MERKYEKIAKEEESVEGRERRLYEASVTGSVNSLKQLMAKDPLTLARAAVTCFNETPLHVAAMLGHLDFASYLLTHKPDMTMALDLRRRSPLHLASANGYVEMVNILLSANPDACLIRDEDGRTPLHLAVMKGEVEVARMLVGARPQVTRYKLDQGETILHSAVKQNRLGALKLLVELVGEVEFVNSKDDYDNTVLHTATALKQYETAKYLVERPEMEVNAVNGNGFTALDIIQHMPRDLKGMEIRESLVKAGALSFGNIPALPGKGHQLMGESGITMVIENPQLSPPPPAAEVLTEAKASTPLRSREKKIRENKKEWTKKKLDALMVAATLIAGMAFQAAVNPPGGVWSEEEDVGEGKKVLAGTSIMAHNYPKWYRLFMECNAVSFVASLCIVFLVVSGVPFVKKRILTWLLMIIMWITLTFMALTYLCSILVIAPTNDGTEAKVTDINDVVGVSLLVWFSLFAFVVLVHIIRFLIWCVREVGKAIIYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D914</accession>
    <name>A0AA39D914_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DYW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_024390</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. PCMP-H subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D914"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000031">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:002536">
        <property type="entry name" value="Tetratricopeptide repeat (TPR)-like superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032867">
        <property type="entry name" value="DYW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046849">
        <property type="entry name" value="E2_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF408">
        <property type="entry name" value="DYW DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14432">
        <property type="entry name" value="DYW_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20430">
        <property type="entry name" value="Eplus_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="155"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="257"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="292"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="DYW" evidence="5">
        <location>
            <begin position="471"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF14432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9675446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="563" mass="62552" checksum="10EC1AAD5DFF887B" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DBC8</accession>
    <name>A0AA39DBC8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_022690</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the DEAD box helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DBC8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd17966">
        <property type="entry name" value="DEADc_DDX5_DDX17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000079">
        <property type="entry name" value="probable ATP-dependent RNA helicase DDX17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="12">
        <location>
            <begin position="294"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="325"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="528"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="22"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="7">
        <location>
            <begin position="294"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="24"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="63"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="96"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="127"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="201"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9677861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="609" mass="64727" checksum="B69CD2D7B15116C5" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DBX6</accession>
    <name>A0AA39DBX6_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_021923</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9676642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DBX6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="120"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9676642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="155" mass="16849" checksum="ECC2969BAFE48B3B" modified="2024-01-24" version="1">MTPNEIWKWVWDWVFGWHCRGVTRKPPGKSVGLRAGISGNLGRTRPIRPMNHQPHILSLSQPQTLGGCQLTGHFQLSKPQAQAQAAHAQFQAQLQAQAQSHAQLHNGWTGNFGVCSPSVSAPGMGSAKRGSQKPPSRPHSSVNATTQLPGLRPWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DDE0</accession>
    <name>A0AA39DDE0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DHHA1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_022894</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9678142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DDE0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.310.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46922">
        <property type="entry name" value="DHHA1 DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46922:SF4">
        <property type="entry name" value="DHHA1 DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="DHH phosphoesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="377"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9678142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="398" mass="43797" checksum="F4521D74937FAFE1" modified="2024-01-24" version="1">MRNYGSSPRTPCPRVTSHFVDVGFPAMSHLPNAAAVLYHYPCPDGAFAALAAHLYLRATSTPTLFFPNRVYSPVSTHHLPLPQISHLYLLDFVGPPDFLPQVSPNVPQVTVLDHHKTALQLLQSWNPSNALKVIDVGRSGATIAFDYFKQRALEEGLLEVAGEFERVRRVFEYVEDADLWRWGLENSKAFSSGLNDMNLEFDVGLNPSLFDQLLSLDLELVINEGMVSLSKKQVLIDEALEQSYEIALGGGTFGHCLAVNADSISELRSELGHQLASKSRNLKLRGIGAVVYRVPELKNDQFLKISLRSVDNEDTTTISQEYGGGGHRNASSFMLSPAEFDKWKFVNAGGHETSSAPPMIVLEEHHASSVMNCIHESSLSSSSMHEEDGRGTSGDMVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DDM7</accession>
    <name>A0AA39DDM7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA mismatch repair proteins mutS family domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_021700</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DDM7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045910">
        <property type="term" value="P:negative regulation of DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001241">
        <property type="entry name" value="Endonuclease MutS2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007696">
        <property type="entry name" value="DNA_mismatch_repair_MutS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036187">
        <property type="entry name" value="DNA_mismatch_repair_MutS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046893">
        <property type="entry name" value="MSSS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005747">
        <property type="entry name" value="MutS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01069">
        <property type="entry name" value="mutS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48466:SF2">
        <property type="entry name" value="OS10G0509000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48466">
        <property type="entry name" value="OS10G0509000 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20297">
        <property type="entry name" value="MSSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005814">
        <property type="entry name" value="MutS_YshD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00533">
        <property type="entry name" value="MUTSd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48334">
        <property type="entry name" value="DNA repair protein MutS, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00486">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair proteins mutS family" evidence="6">
        <location>
            <begin position="530"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="712"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="192"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="381"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="715"/>
            <end position="742"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9679869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="858" mass="94596" checksum="7A29FF5E492D9756" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DE00</accession>
    <name>A0AA39DE00_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_019567</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DE00"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052160">
        <property type="entry name" value="Gypsy_RT_Integrase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47266">
        <property type="entry name" value="ENDONUCLEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="20"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9680289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="193" mass="22243" checksum="F35F5A9A61A08E14" modified="2024-01-24" version="1">MCRSCEKCQRLGKLTKRNQMPMNPILIVDLFDVWGIDFMGPFPMSFGNSYILVGVDYVSKWVEAIPYKHNDHKVVLKFLKEHIFSRFGVPKAIIGDGGTHFCNKPFETLLAKYGVKHKVATPYHPQTSGQVELANREIKNILMKVVTTSRKDWSIKLHDSLWAYRTAYKTILSMSPYRLVYGKACHLPVEVEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DE93</accession>
    <name>A0AA39DE93_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pectinesterase inhibitor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_021061</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the PMEI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DE93"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046910">
        <property type="term" value="F:pectinesterase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd15798">
        <property type="entry name" value="PMEI-like_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.40:FF:000005">
        <property type="entry name" value="Pectin methylesterase inhibitor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.40">
        <property type="entry name" value="Invertase/pectin methylesterase inhibitor family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035513">
        <property type="entry name" value="Invertase/methylesterase_inhib"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006501">
        <property type="entry name" value="Pectinesterase_inhib_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051955">
        <property type="entry name" value="PME_Inhibitor"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01614">
        <property type="entry name" value="PME_inhib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31080">
        <property type="entry name" value="PECTINESTERASE INHIBITOR-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31080:SF117">
        <property type="entry name" value="PLANT INVERTASE_PECTIN METHYLESTERASE INHIBITOR SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04043">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00856">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101148">
        <property type="entry name" value="Plant invertase/pectin methylesterase inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041321138" description="Pectinesterase inhibitor domain-containing protein" evidence="3">
        <location>
            <begin position="30"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="Pectinesterase inhibitor" evidence="4">
        <location>
            <begin position="37"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9679027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="202" mass="21609" checksum="C8CB3C1222C69029" modified="2024-01-24" version="1">MEGSFFSFHFLPALLIFLSLTSYINLSSAARPAADKASTEFIRTSCGTTTYPRLCYTSLSAHASVIQTSPKLLADTALAVTLSTARSTSSLMSKMVQSHGLKPREVAAMQDCVEELSDSVDQLRKAMGEMTQIKGSNFGPVMNDIQTWVSAALTDEDTCTDGFAGNAMNGNLKTAVRARVVNMAHLTSNALGLINGYASLHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DF60</accession>
    <name>A0AA39DF60_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_018571</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DF60"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080090">
        <property type="term" value="P:regulation of primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000218">
        <property type="entry name" value="Myb transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015495">
        <property type="entry name" value="Myb_TF_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47999:SF121">
        <property type="entry name" value="MYB TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47999">
        <property type="entry name" value="TRANSCRIPTION FACTOR MYB8-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="4"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="7"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="57"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="57"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9682673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="243" mass="27916" checksum="2757D37C85242E41" modified="2024-01-24" version="1">MDGPSGVRKGAWTREEDVLLRTCIEKYGEGKWHQVPFRSGLNRCRKSCRLRWLNYLRPDIKRGEFTTDEVDLIIRLHKLLGNRWALIAGRLPGRTSNDVKNYWNTHLNKKCFGKAGKDVAEKTAKVTVIKPRPRTFYKNLSYLKGKAAKESDVQSADHTLSKPHSTPPLSEDGFSWWDSLFSGKEGHEESTCFRDGSAKESISSFWDEETPAATKVGETFLEEKESDWNHFSFDMDLWDLLNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DFP0</accession>
    <name>A0AA39DFP0_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_019717</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DFP0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="20"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9680467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="98" mass="10961" checksum="3BDF492D82389CF1" modified="2024-01-24" version="1">MLRMQLMCMKQSMDKQTEASSSRRSSKSSQLWDIYAMCKSMNNQLKIVHQQCDRSIATWKALHPNALPPTPSQTDDEENGDDDGGDEKGEDAPANGNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DHQ2</accession>
    <name>A0AA39DHQ2_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_016484</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DHQ2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044550">
        <property type="term" value="P:secondary metabolite biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20658">
        <property type="entry name" value="CYP79"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000037">
        <property type="entry name" value="Cytochrome P450 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47944">
        <property type="entry name" value="CYTOCHROME P450 98A9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47944:SF4">
        <property type="entry name" value="OS09G0441700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8 9" id="KW-0349">Heme</keyword>
    <keyword evidence="6 8 9" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="8">
        <location>
            <position position="501"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9684010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="563" mass="63846" checksum="CC4572EE612F36E0" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DIU5</accession>
    <name>A0AA39DIU5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_004220</name>
        <name evidence="15" type="ORF">PVL29_006269</name>
        <name evidence="14" type="ORF">PVL29_014684</name>
        <name evidence="13" type="ORF">PVL29_017571</name>
        <name evidence="12" type="ORF">PVL29_019668</name>
        <name evidence="11" type="ORF">PVL29_026122</name>
    </gene>
    <organism evidence="13 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9689150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DIU5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.370:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 17.6-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000020">
        <property type="entry name" value="Transposon Tf2-6 polyprotein-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041373">
        <property type="entry name" value="RT_RNaseH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17917">
        <property type="entry name" value="RT_RNaseH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="10">
        <location>
            <begin position="813"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="139"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="99"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9672771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9680409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9685584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9689150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9700858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9702383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1123" mass="130554" checksum="57E2D38983E25AFD" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DJD6</accession>
    <name>A0AA39DJD6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ALOG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_015163</name>
        <name evidence="11" type="ORF">PVL29_015164</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the plant homeotic and developmental regulators ALOG protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DJD6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009299">
        <property type="term" value="P:mRNA transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009416">
        <property type="term" value="P:response to light stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040222">
        <property type="entry name" value="ALOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006936">
        <property type="entry name" value="ALOG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31165">
        <property type="entry name" value="PROTEIN G1-LIKE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31165:SF10">
        <property type="entry name" value="PROTEIN LIGHT-DEPENDENT SHORT HYPOCOTYLS 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04852">
        <property type="entry name" value="ALOG_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51697">
        <property type="entry name" value="ALOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="ALOG" evidence="9">
        <location>
            <begin position="27"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="13"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9686138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9686139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="167" mass="18676" checksum="05C6BDBDD5A15C82" modified="2024-01-24" version="1">MSSERGKDLAEGSSRSPGDQQSVTPSRYESQKRRDWNTFGQYLKNQRPPVPLSQCNCNHVLDFLRYLDQFGKTKVHLQGCMFFGQPEPPAPCTCPLRQAWGSLDALVGRLRAAYEENGGSPETNPFASGAIRIYLREVRECQSKARGIPYKKKKKKAGESTSAGQFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DK56</accession>
    <name>A0AA39DK56_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_017188</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C48 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DK56"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.395.10">
        <property type="entry name" value="Adenoviral Proteinase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="4">
        <location>
            <begin position="24"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9685057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="103" mass="12141" checksum="29975771E15AA120" modified="2024-01-24" version="1">MSIFIILYCVKVNVLSTIIRIQERRPLMDMTEFEFVVPEVVQQLNPMDCDIFVIKFMQLWSKGGLSRAIVNDKVIKYKEKLLTQLIISLKNEVRENVYQAMNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DL09</accession>
    <name>A0AA39DL09_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Aminotransferase-like plant mobile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_014995</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9685897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DL09"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010073">
        <property type="term" value="P:meristem maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019557">
        <property type="entry name" value="AminoTfrase-like_pln_mobile"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044824">
        <property type="entry name" value="MAIN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46033">
        <property type="entry name" value="PROTEIN MAIN-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46033:SF1">
        <property type="entry name" value="PROTEIN MAIN-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10536">
        <property type="entry name" value="PMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase-like plant mobile" evidence="2">
        <location>
            <begin position="62"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="485"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="488"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="515"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9685897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="632" mass="71180" checksum="8A0E45A8041DF40C" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DLG1</accession>
    <name>A0AA39DLG1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PGG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_014673</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9689138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DLG1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000676">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026961">
        <property type="entry name" value="PGG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24177">
        <property type="entry name" value="CASKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24177:SF103">
        <property type="entry name" value="PGG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13962">
        <property type="entry name" value="PGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="682"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="729"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="762"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="92"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="PGG" evidence="4">
        <location>
            <begin position="640"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="293"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="443"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="303"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="448"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="472"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9689138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="809" mass="89854" checksum="FDC1FCBE1E7B26D1" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DNY9</accession>
    <name>A0AA39DNY9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">40S ribosomal protein S15a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_013821</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DNY9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.30:FF:000001">
        <property type="entry name" value="40S ribosomal protein S15a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000630">
        <property type="entry name" value="Ribosomal_uS8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035987">
        <property type="entry name" value="Ribosomal_uS8_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003115">
        <property type="entry name" value="PRK04034.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11758">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S15A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00410">
        <property type="entry name" value="Ribosomal_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56047">
        <property type="entry name" value="Ribosomal protein S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9691748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="148" mass="16874" checksum="886B36F682405E2E" modified="2024-01-24" version="1">MTASTNIQQPFSTRIVIVSVLNDALKSMYNAEKRRKRQVMIKPSSKVIVKFLLVIQKHGYIGEFEYVDDHRSGKIVVELNGKLNKCDVISPHFDVGVKNIEPWTTRLLPSRKFGYIVLTTFASIMDHEEAPKMLVAKFLVSFTSHSFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DPB0</accession>
    <name>A0AA39DPB0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein ROOT HAIR DEFECTIVE 3 homolog</fullName>
            <ecNumber evidence="9">3.6.5.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Protein SEY1 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_011246</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Probable GTP-binding protein that may be involved in cell development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Endoplasmic reticulum membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the TRAFAC class dynamin-like GTPase superfamily. GB1/RHD3 GTPase family. RHD3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.-" evidence="9"/>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DPB0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016320">
        <property type="term" value="P:endoplasmic reticulum membrane fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01851">
        <property type="entry name" value="GBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002271">
        <property type="entry name" value="Protein ROOT HAIR DEFECTIVE 3 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03109">
        <property type="entry name" value="Sey1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030386">
        <property type="entry name" value="G_GB1_RHD3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008803">
        <property type="entry name" value="RHD3/Sey1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046758">
        <property type="entry name" value="Sey1/RHD3-like_3HB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45923">
        <property type="entry name" value="PROTEIN SEY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45923:SF2">
        <property type="entry name" value="PROTEIN SEY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05879">
        <property type="entry name" value="RHD3_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20428">
        <property type="entry name" value="Sey1_3HB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51715">
        <property type="entry name" value="G_GB1_RHD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="topological domain" description="Cytoplasmic" evidence="9">
        <location>
            <begin position="1"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="topological domain" description="Lumenal" evidence="9">
        <location>
            <begin position="702"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="9">
        <location>
            <begin position="726"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="domain" description="GB1/RHD3-type G" evidence="11">
        <location>
            <begin position="37"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="760"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="786"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="796"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="47"/>
            <end position="54"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9692063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="813" mass="90093" checksum="E08A886204B27551" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DRM0</accession>
    <name>A0AA39DRM0_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_010256</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the pseudouridine synthase TruB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DRM0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031429">
        <property type="term" value="C:box H/ACA snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000495">
        <property type="term" value="P:box H/ACA sno(s)RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990481">
        <property type="term" value="P:mRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031118">
        <property type="term" value="P:rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031120">
        <property type="term" value="P:snRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02572">
        <property type="entry name" value="PseudoU_synth_hDyskerin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21148">
        <property type="entry name" value="PUA_Cbf5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2350.10:FF:000001">
        <property type="entry name" value="H/ACA ribonucleoprotein complex subunit CBF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.10">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="entry name" value="PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012960">
        <property type="entry name" value="Dyskerin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002501">
        <property type="entry name" value="PsdUridine_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004802">
        <property type="entry name" value="tRNA_PsdUridine_synth_B_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032819">
        <property type="entry name" value="TruB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004521">
        <property type="entry name" value="Uncharacterised_CHP00451"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00425">
        <property type="entry name" value="CBF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003280">
        <property type="entry name" value="PRK04270.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00451">
        <property type="entry name" value="unchar_dom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23127">
        <property type="entry name" value="CENTROMERE/MICROTUBULE BINDING PROTEIN CBF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23127:SF0">
        <property type="entry name" value="H_ACA RIBONUCLEOPROTEIN COMPLEX SUBUNIT DKC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08068">
        <property type="entry name" value="DKCLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16198">
        <property type="entry name" value="TruB_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01509">
        <property type="entry name" value="TruB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01136">
        <property type="entry name" value="DKCLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dyskerin-like" evidence="5">
        <location>
            <begin position="51"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="PUA" evidence="4">
        <location>
            <begin position="300"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="445"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="445"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="499"/>
            <end position="536"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9694691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="586" mass="65662" checksum="4E54B696478930DF" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DSZ6</accession>
    <name>A0AA39DSZ6_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_011994</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9693082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DSZ6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9693082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="85" mass="9598" checksum="375ABFB1358C8DBC" modified="2024-01-24" version="1">MLDQKSTMKQALHFGMEGLGIFLHKIQNTMKVFVSPTIYATVQSKAAFVLSESVEVLKHLAKSDSAVIYIIEESRENIIAERTRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DU51</accession>
    <name>A0AA39DU51_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Vacuolar protein sorting-associated protein 62</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_009000</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DU51"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009291">
        <property type="entry name" value="Vps62"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48173">
        <property type="entry name" value="GNK2-HOMOLOGOUS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48173:SF1">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06101">
        <property type="entry name" value="Vps62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9697043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="569" mass="63484" checksum="C3B1E6F3C8120611" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DVW9</accession>
    <name>A0AA39DVW9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Exopolygalacturonase-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_005496</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the glycosyl hydrolase 28 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9699656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DVW9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004650">
        <property type="term" value="F:polygalacturonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000004">
        <property type="entry name" value="Pectin lyase-like superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000743">
        <property type="entry name" value="Glyco_hydro_28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31375">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00295">
        <property type="entry name" value="Glyco_hydro_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00502">
        <property type="entry name" value="POLYGALACTURONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0134">Cell wall</keyword>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6 9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041280718" description="Exopolygalacturonase-like" evidence="11">
        <location>
            <begin position="23"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9699656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="428" mass="45953" checksum="DDEF69165C7B99EA" modified="2024-01-24" version="1">MATARRIILYSFLLCSFALVSCRANAGSHPRVGRGRGSVAHHGGGGGRGPIPGETIFNVLQYGAKPGGKKDSTEAFMKAWVAACHWRGKARLLIPQGIFLIGQVTFQGPCNSPTPIIVQVAGTLKAVTDISEFASPEWVTFEHINGLIVTGGGTFDGQGDVVWKYNDCRRNSDCQLLPTSIKFNSITNGNLRGINSVNAKSFHIAMNRCQNFRAFGLRITAPEDSPNTDGIHISSSNFVKVSKSIISTGDDCISIGQGSTNISINKVTCGPGHGISIGSLGKYPDEKDVMGIIVKNSTLLNTDNGLRIKTWPGSPPSQASGILFQDIIIKNVKNPIIIDQLYCPSGSSCRTQPSRVRISNIHYRNIWGTSSSPLAVNLMCSPQFPCQNVELFDINLRYSGKRRVSALTSSCSNVKAGFGGVQIPPPCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DW61</accession>
    <name>A0AA39DW61_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Dienelactone hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_008477</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DW61"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000178">
        <property type="entry name" value="Carboxymethylenebutenolidase homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002925">
        <property type="entry name" value="Dienelactn_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17630">
        <property type="entry name" value="DIENELACTONE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17630:SF44">
        <property type="entry name" value="PROTEIN AIM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01738">
        <property type="entry name" value="DLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dienelactone hydrolase" evidence="3">
        <location>
            <begin position="29"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9696262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="237" mass="25678" checksum="3C5C2120A7811B70" modified="2024-01-24" version="1">MSGPQCCENPPVLSPSCGAGSVAEIGGLKAYFTGPPDSKLVILLVSDILGYEAPNLRKLADKVAAAGFYVVVPDFFHGDPFVPETKTLPVWIKYHGPDKGFEDVKPIIAALRSKGINSIGAAGFCWGAKVVVELAKVGDIQAAVLLHPSFTTVDDIKEIKAPISILGAETDHVSPPELVKQFEEVLATKPEIKSFVKIFPGVAHGWAVRYKVDDEEAVKRAEEAQQNMMDWFIEYVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DWS9</accession>
    <name>A0AA39DWS9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Disease resistance RPP13-like protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_007840</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057135">
        <property type="entry name" value="At4g27190-like_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056789">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766:SF51">
        <property type="entry name" value="DISEASE RESISTANCE RPP13-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766">
        <property type="entry name" value="PLANT BROAD-SPECTRUM MILDEW RESISTANCE PROTEIN RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25019">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23247">
        <property type="entry name" value="LRR_RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0611">Plant defense</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="7">
        <location>
            <begin position="9"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="6">
        <location>
            <begin position="180"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="9">
        <location>
            <begin position="438"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="domain" description="R13L1/DRL21-like LRR repeat region" evidence="10">
        <location>
            <begin position="695"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein At4g27190-like leucine-rich repeats" evidence="8">
        <location>
            <begin position="1018"/>
            <end position="1151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF25019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9698964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1357" mass="154038" checksum="5D8785C0525F49E7" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DX64</accession>
    <name>A0AA39DX64_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein transport protein SEC23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_006502</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10 11">Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasmic vesicle</location>
            <location evidence="11">COPII-coated vesicle membrane</location>
            <topology evidence="11">Peripheral membrane protein</topology>
            <orientation evidence="11">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Endoplasmic reticulum membrane</location>
            <topology evidence="11">Peripheral membrane protein</topology>
            <orientation evidence="11">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the SEC23/SEC24 family. SEC23 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DX64"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090110">
        <property type="term" value="P:COPII-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11287">
        <property type="entry name" value="Sec23_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.730:FF:000005">
        <property type="entry name" value="Protein transport protein SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.380:FF:000001">
        <property type="entry name" value="Protein transport protein SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1670:FF:000006">
        <property type="entry name" value="Protein transport protein SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000014">
        <property type="entry name" value="Protein transport protein SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000008">
        <property type="entry name" value="Protein transport protein SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1670">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.730">
        <property type="entry name" value="Sec23/Sec24 helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012990">
        <property type="entry name" value="Beta-sandwich_Sec23_24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007123">
        <property type="entry name" value="Gelsolin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036180">
        <property type="entry name" value="Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037364">
        <property type="entry name" value="Sec23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006900">
        <property type="entry name" value="Sec23/24_helical_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036175">
        <property type="entry name" value="Sec23/24_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006896">
        <property type="entry name" value="Sec23/24_trunk_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037550">
        <property type="entry name" value="Sec23_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006895">
        <property type="entry name" value="Znf_Sec23_Sec24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036174">
        <property type="entry name" value="Znf_Sec23_Sec24_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11141">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11141:SF0">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00626">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08033">
        <property type="entry name" value="Sec23_BS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04815">
        <property type="entry name" value="Sec23_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04811">
        <property type="entry name" value="Sec23_trunk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04810">
        <property type="entry name" value="zf-Sec23_Sec24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82754">
        <property type="entry name" value="C-terminal, gelsolin-like domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81811">
        <property type="entry name" value="Helical domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82919">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 11" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="4 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 11" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11" id="KW-0813">Transport</keyword>
    <keyword evidence="5 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zinc finger Sec23/Sec24-type" evidence="13">
        <location>
            <begin position="55"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 trunk" evidence="14">
        <location>
            <begin position="132"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 beta-sandwich" evidence="16">
        <location>
            <begin position="409"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 helical" evidence="15">
        <location>
            <begin position="527"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="12">
        <location>
            <begin position="639"/>
            <end position="727"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU365030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF04811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF04815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF08033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9701186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="771" mass="84892" checksum="A1ED5E1B29121198" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DYE1</accession>
    <name>A0AA39DYE1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PVL29_006682</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">May play a role in signal transduction pathways that involve calcium as a second messenger.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DYE1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000004">
        <property type="entry name" value="Calcium-dependent protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000050">
        <property type="entry name" value="Calcium-dependent protein kinase 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001864">
        <property type="entry name" value="Calcium-dependent protein kinase SK5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001294">
        <property type="entry name" value="CDPK-related kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="55"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="18">
        <location>
            <begin position="356"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="18">
        <location>
            <begin position="428"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="18">
        <location>
            <begin position="465"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00058232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ9701438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="554" mass="63052" checksum="D5C7F9A22D400280" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DZG8</accession>
    <name>A0AA39DZG8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein phosphatase 1 regulatory subunit 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_008247</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9699562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DZG8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025875">
        <property type="entry name" value="Leu-rich_rpt_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050836">
        <property type="entry name" value="SDS22/Internalin_LRR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46652:SF7">
        <property type="entry name" value="LEUCINE-RICH REPEAT AND IQ DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46652">
        <property type="entry name" value="LEUCINE-RICH REPEAT AND IQ DOMAIN-CONTAINING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12799">
        <property type="entry name" value="LRR_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="287"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="287"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9699562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="446" mass="50050" checksum="86D07C36397B1A81" modified="2024-01-24" version="1">MARLSSKQILQENQTDDHNAISALSLNHKALSHISCLTDFKNLERLDLSFNNLTSLQDLKLCVNLKWLSVLQNKLQSLEGIKELSKLTVLNAGKNKLRSMDEVKSLVSLRALILNDNEIGSICRLDRMKDLNTLVLSRNPVHEIGESLVKLKSITKLSLSKCQIQSIGSSLKSCIELKELRLAHNDIKTLPAELAYNTKLQNLDLGNNLITSWSDLKVIRSLVNLKNFNLQGNPIAEKEKLAKKIKRLLPNLQIFNARPTDKITKYEKGDKVDDFPLNVSTELEVKKKDKRDHGRAEKNKHNSMIESGVAHLVNGDLDVEKELKKKKRKANDEAKKKVPIHKEDHTRVERELNKKARKVERGGLSAIDDGETPFMELFAAETAENSKYSVEDRTDKAFQNSNSAGGSVSFGAKKKKTKRWGISPSIQLLSLPVEVGLGGPSTWDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DZR1</accession>
    <name>A0AA39DZR1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_005439</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9704156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DZR1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22157">
        <property type="entry name" value="F-box_AtFBW1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055290">
        <property type="entry name" value="At3g26010-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056592">
        <property type="entry name" value="Beta-prop_At3g26010-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017451">
        <property type="entry name" value="F-box-assoc_interact_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01640">
        <property type="entry name" value="F_box_assoc_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35546:SF21">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35546">
        <property type="entry name" value="F-BOX PROTEIN INTERACTION DOMAIN PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24750">
        <property type="entry name" value="b-prop_At3g26010-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9704156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="377" mass="43080" checksum="92FD8FC93E28E8A8" modified="2024-01-24" version="1">MESDDICMDVLSRLPTKTLLGLKCVCKRWHRIISDRSFIQDLLQRPEPLAGFFFQERYQWCAEDISTISYIPASMEGTQVQQTIFSFLPEDVVILGSCNGLVCCRSVFPSPDPSIFICNPSNKQWIRLQETTPDKESSYGLAFNPFQSTMDMSTNFKVVRVSQAQTDMDDSYFSFEIYSSQAGAWKKSKEICQCNHNLFKNKGIFAGGILHWLTDGDQVLSFNIENELSWLISAPFPTIHLTAIPEMCIGESRGRLHYIMISEDGLQVWALEDYYDSNWSLVHSTTLEVMEEENSQLLYNTRERVAQRISIDSIPWMDPLAFKDDLLLMRVSTKIILYCIGTRKMKELCNLSKLGRNSIFGPLVIPYSLSLAPLSQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E0E3</accession>
    <name>A0AA39E0E3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA-directed RNA polymerase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_006049</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">DNA-dependent RNA polymerase which catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E0E3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055029">
        <property type="term" value="C:nuclear DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04329">
        <property type="entry name" value="RNAP_II_Rpb7_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000043">
        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.120:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.120">
        <property type="entry name" value="RNA polymerase Rpb7-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036898">
        <property type="entry name" value="RNA_pol_Rpb7-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045113">
        <property type="entry name" value="Rpb7-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12709">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II, III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12709:SF3">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE V SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88798">
        <property type="entry name" value="N-terminal, heterodimerisation domain of RBP7 (RpoE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU369086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9700552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="177" mass="20044" checksum="8D6B0D35AD1133C2" modified="2024-01-24" version="1">MFLKVQLPWNVIIPAECLDAKGLMLQRSIIIRLLDAFSNKKATQELGYFLAVTTLENIGEGKVRQHSGDVLFPVVFSCVTFKLFRGEILDGVVHKVLKHGVILRCGPVENIYLSCQKMPDYRYVPGENPVFLNEKLSKIEKDVVVRFIVMGTKWLEAEREFQALVSLEGDYLGPLSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E4H4</accession>
    <name>A0AA39E4H4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Fatty acid hydroxylase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_001971</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sterol desaturase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E4H4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006694">
        <property type="entry name" value="Fatty_acid_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050307">
        <property type="entry name" value="Sterol_Desaturase_Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11863">
        <property type="entry name" value="STEROL DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04116">
        <property type="entry name" value="FA_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="35"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid hydroxylase" evidence="7">
        <location>
            <begin position="131"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9706764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="309" mass="35873" checksum="A14947D53DD4065F" modified="2024-01-24" version="1">MLPYKTLQEAEAFLGRNLTFPETIWLNYSANKSDYFLYCHNILFLFLIFSVVPLPLVFMELSRSSGLDKFKIQPKVRLSSADVFRCYKDVMSMFFFIVGPLQLVSYPSIKMIGIRTSLPLPSGWEILMQLAVYFMVEDFTNYWIHRFLHCKWGYEKIHRVHHEYTAPIGFAAPYAHWAEILILGIPSFLGPAMAPGHMITFWLWIALRQIEAIDTHSGYDFPWSPTKYIPFYGGADHHDYHHYVGGQSQSNFASVFTYCDYIYGTDKGYRYQKKLLAKVTFSAMYFLLEYDHGLSAALLGFLPCLILNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YHI5</accession>
    <name>A0AA38YHI5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_026859</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YHI5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050651">
        <property type="entry name" value="Plant_Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47947:SF26">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47947">
        <property type="entry name" value="CYTOCHROME P450 82C3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0349">Heme</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9670576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="250" mass="29013" checksum="222DD359C0DD22FA" modified="2024-01-24" version="1">MMIFSLRFGSRLVVIISSPSTVEECFTKNDIIFANRPIFLFGKYIGYNNTTVTSAPYGEHWRNLRRLSALEIFSPNWFNMFIGTRRNEIKILLKRLSQNSRDNFARVELRLMFTELTCDIIMRMAAGKRFYGEDVDFEEAKHFHEELVKINRMKDVIFQGLIDEHRSPTSLVNKNTMIDHLLSMQKPEPEYYTDEIIKGLALDLILAGTDTTATTIEWAILSLLLDHPDVLKKARVELDALVGKDRLMEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YIX4</accession>
    <name>A0AA38YIX4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">FYVE-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_027314</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9671267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YIX4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033565">
        <property type="term" value="C:ESCRT-0 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15760">
        <property type="entry name" value="FYVE_scVPS27p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47794">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47794:SF1">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FYVE-type" evidence="7">
        <location>
            <begin position="8"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="229"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="155"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="164"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9671267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="289" mass="31304" checksum="7423543551A86478" modified="2024-01-24" version="1">MSVEPPPFQEAARCDVCKCSFNTFRRRHHCRCCGRTLCHEHSSNQMALPQFGLHMSVRVCSDCFNDSSRLGKVDPVASLDGINSVVDSVATLNINPNVDLKTDEPTAVHNAVSGISDCKCGMPLCICEVPAAPVETVPLQRKTIAISTTHLNPKPKKTDCNLKNRGSTSNSKHGLVSNLGQVSNSSLDKPQMDYDANGEGLREAIKNSDTAAVKKLLSQGVDANYCDKQGLSVLHLAALFNQTDIVFILMESGASLEHKNAQGETPLDCAPATLQYKMQKKMEEGGQKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YJG3</accession>
    <name>A0AA38YJG3_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_025338</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YJG3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046394">
        <property type="term" value="P:carboxylic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.10.10:FF:000002">
        <property type="entry name" value="D-alanine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.10:FF:000008">
        <property type="entry name" value="D-amino-acid transaminase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.10.10">
        <property type="entry name" value="D-amino Acid Aminotransferase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001544">
        <property type="entry name" value="Aminotrans_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036038">
        <property type="entry name" value="Aminotransferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043132">
        <property type="entry name" value="BCAT-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043131">
        <property type="entry name" value="BCAT-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050571">
        <property type="entry name" value="Class-IV_PLP-Dep_Aminotrnsfr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42743">
        <property type="entry name" value="AMINO-ACID AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42743:SF8">
        <property type="entry name" value="OS01G0238500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01063">
        <property type="entry name" value="Aminotran_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56752">
        <property type="entry name" value="D-aminoacid aminotransferase-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9671577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="351" mass="38781" checksum="F2C2883832602CAC" modified="2024-01-24" version="1">MENCNSPSDQKSDAVTGAEVERDTDFRVHVFSSSSELLEKLHEKWDSGKEKPYPAMYSSVFGGIILDPAMMVIPIDDHMVHRGHGVFDTSIILNGYLYELDAHLDRFLRSAAKAKISSPFPRSTLRSILVHLTAASRCTEGTLRFWLTSGPGNFLLSPSGCPTSAFYAVVIKDKIYQHREGVKVITSTIPMKSPLFATTKNVNYLPNVLSVMEAEEKGAFASIWVDEEGHIAEGPNVNVAFITHDKELILPFFDKILSGCTAKRLLELAPKLVEQGRLKDVRTANLTVEEAKGSAEMMYVGSTLPLLPIVMWDEKAIGDGLVGELTMALSDLLWDDMVAGPETQRLLVTYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YK75</accession>
    <name>A0AA38YK75_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_025575</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YK75"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.430:FF:000003">
        <property type="entry name" value="Probable disease resistance protein At5g66910"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050905">
        <property type="entry name" value="Plant_NBS-LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463:SF202">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="166"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9671989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1004" mass="113483" checksum="17CCF23DFDEA4FF6" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YM28</accession>
    <name>A0AA38YM28_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">LOB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_026290</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LOB domain-containing protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YM28"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004883">
        <property type="entry name" value="LOB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31301:SF58">
        <property type="entry name" value="LOB DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31301">
        <property type="entry name" value="LOB DOMAIN-CONTAINING PROTEIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03195">
        <property type="entry name" value="LOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50891">
        <property type="entry name" value="LOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LOB" evidence="4">
        <location>
            <begin position="12"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="125"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="92"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9672961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="162" mass="17825" checksum="DB438A04C5BC8FB8" modified="2024-01-24" version="1">MKESGRKQGAVSPCAACKLLRRRCAQDCVFAPYFPADEPHKFASVHKVFGASNVNKMLQELPEHQRSDAVSSMVYEANARVRDPVYGCVGAISSLQQQIDALQTQLALVQAEVVQMRMNQFTFSTSNQATDSPENASHTSGLAASPQTKSFFSNMVESLWSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YMU5</accession>
    <name>A0AA38YMU5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_023137</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YMU5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48017">
        <property type="entry name" value="OS05G0424000 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="105"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="130"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="191"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="229"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="253"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="294"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="342"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="387"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="429"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="456"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="489"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="640"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="755"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="845"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="889"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="931"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="958"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="991"/>
            <end position="1017"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="8">
        <location>
            <begin position="102"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="8">
        <location>
            <begin position="610"/>
            <end position="1013"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9673381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1029" mass="112962" checksum="FA05ECC075FC4B3B" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YNH9</accession>
    <name>A0AA38YNH9_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_023293</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YNH9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36617:SF5">
        <property type="entry name" value="OS05G0421675 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36617">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9673648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="145" mass="16590" checksum="9284680F77C14A02" modified="2024-01-24" version="1">MGHLVTPSFGFVVGVGKKVRFWKDKWCGTTPLCEDFPSLFALTTSKEAWVNEVLIAAGEMGESWTPRFNRPFNDWELEEVERLFCHLDGKKVSVDEENRVRWMDSNDGVFSVKSLYRALQLVSLASFSSKIIWNSCVQPKLSFFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YQI7</accession>
    <name>A0AA38YQI7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_023990</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YQI7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF78">
        <property type="entry name" value="OS12G0587133 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9674791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="602" mass="68896" checksum="DBF0DBA13DDCAD71" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YRD0</accession>
    <name>A0AA38YRD0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Exonuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_024185</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the REXO1/REXO3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YRD0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06145">
        <property type="entry name" value="REX1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000019">
        <property type="entry name" value="RNA exonuclease NEF-sp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034922">
        <property type="entry name" value="REX1-like_exo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047021">
        <property type="entry name" value="REXO1/3/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Ribonucl_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801">
        <property type="entry name" value="RNA EXONUCLEASE REXO1 / RECO3 FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801:SF157">
        <property type="entry name" value="SMALL RNA DEGRADING NUCLEASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exonuclease" evidence="8">
        <location>
            <begin position="216"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="9"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9675133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="576" mass="64402" checksum="36AB90AE01940CC7" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YSG8</accession>
    <name>A0AA38YSG8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase</fullName>
            <ecNumber evidence="3">3.2.2.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_024569</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>NAD(+) + H2O = ADP-D-ribose + nicotinamide + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16301"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57967"/>
            <dbReference type="EC" id="3.2.2.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:16302"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the disease resistance TIR-NB-LRR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.6" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YSG8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061809">
        <property type="term" value="F:NAD+ nucleosidase activity, cyclic ADP-ribose generating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050832">
        <property type="term" value="P:defense response to fungus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043068">
        <property type="term" value="P:positive regulation of programmed cell death"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10140:FF:000007">
        <property type="entry name" value="Disease resistance protein (TIR-NBS-LRR class)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32009:SF39">
        <property type="entry name" value="TIR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32009">
        <property type="entry name" value="TMV RESISTANCE PROTEIN N-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TIR" evidence="10">
        <location>
            <begin position="18"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9675702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="168" mass="19031" checksum="FD0986CD9089898E" modified="2024-01-24" version="1">MASSSTRTASSSTSSISRNYDVFLSFQGEDTRKNFTDHLYTALVACGIQTFRDNEELDKGGDIASDLSRAIEESRIFIIIFSKNYAYSTWCLNELVKIVECMRQKDIMVLPVFYHVDPSDVRKQRGNFGDALAHHEGDADQEKKQMVQKWRIALTKAADLSGCHVDHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YSL5</accession>
    <name>A0AA38YSL5_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_024641</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YSL5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9675797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="94" mass="10245" checksum="CD41763CEA3355C1" modified="2024-01-24" version="1">MARRVELQPRVDEVETGAVAAIRQYPENAAPITGDRRDWFSYRLGVEEDGDENKEAAAVAAAMLSLRSSVVVPTAMLLMLMLALSALEVPISEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z0X9</accession>
    <name>A0AA38Z0X9_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_019609</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z0X9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9680341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="84" mass="9115" checksum="B79FD832DC88B2FE" modified="2024-01-24" version="1">MIGSHSSTTMATTLLPLAVIVMLFRHLLPLLYHPVDSATTCVSSEWNWPSHADGAPPSSLFSKPLRVRNAMVGLETGGEHLYVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z1C0</accession>
    <name>A0AA38Z1C0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein ECERIFERUM 1-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_019651</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sterol desaturase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z1C0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021940">
        <property type="entry name" value="CER1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006694">
        <property type="entry name" value="Fatty_acid_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050307">
        <property type="entry name" value="Sterol_Desaturase_Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11863">
        <property type="entry name" value="STEROL DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12076">
        <property type="entry name" value="CER1-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04116">
        <property type="entry name" value="FA_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="18"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="127"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="183"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid hydroxylase" evidence="7">
        <location>
            <begin position="134"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="Very-long-chain aldehyde decarbonylase CER1-like C-terminal" evidence="8">
        <location>
            <begin position="452"/>
            <end position="605"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9680387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="605" mass="69793" checksum="5744E15FAC5E7099" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z1X8</accession>
    <name>A0AA38Z1X8_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_019884</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z1X8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33918:SF3">
        <property type="entry name" value="CYTOCHROME P450 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33918">
        <property type="entry name" value="OS01G0704200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="151"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="191"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="226"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="297"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9680702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="332" mass="36895" checksum="A5FCDD1B0B1E9F16" modified="2024-01-24" version="1">MASLAQAPSTMASVTVPKVHYRGMKLFQPSISLHRFTFPHIRIKHPPVCCTKLTPWEPSPVTYSPTDEKGSDFLRGTSNIFETLNSDDTAEAPVTNTEELIHTSNQPSGQLQFLKWPMWLLGPSLLLGTGMVPTLWLPISSIFLGPNIASLLSLIGLDCIFNLGATLFLLMADACARPKNLAQPLNSEAPFSYQFWNMVATITGFVVPLIVFFGSQNGFFQPQLPFIPFAVLLGPYLLLLSVQILTEMLTWHWQSPVWLVTPVVYEAYRVLQLMRGLKLGAELSAPAWMMHTVRGLVCWWVLILGMQLMRVAWFAGFTAGAWPQKSSTLADG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z2P8</accession>
    <name>A0AA38Z2P8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cytochrome P450 76AD1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_020016</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z2P8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24299">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24299:SF59">
        <property type="entry name" value="CYTOCHROME P450 SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041329275" description="Cytochrome P450 76AD1-like protein" evidence="1">
        <location>
            <begin position="16"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9680879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="219" mass="24242" checksum="9E1537E422B5F7F5" modified="2024-01-24" version="1">MDYITFLLLLSFVWTCIHLLKLSPIGRKPGTAGLPPRPRPLPIIGNILKLGDKPYRSLANLFKTYGPVMSLKLGSTATIVISSSETAKEVLHRNDQAFSSRRVLDAVRAHNHHESSVVWVPASAHWRKIRKICTTQIFSVQHLDASQGLRRKIVQELLDHVEECCGRGCAVNIGGAAFTANLNLLSNIIFSINLAHHDSNFSQEFKNIVWGVMGILSGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z3R8</accession>
    <name>A0AA38Z3R8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">RCK N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_020429</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9681552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z3R8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015386">
        <property type="term" value="F:potassium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1530.20:FF:000007">
        <property type="entry name" value="K(+) efflux antiporter 2 chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004771">
        <property type="entry name" value="K/H_exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00932">
        <property type="entry name" value="2a37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46157:SF2">
        <property type="entry name" value="K(+) EFFLUX ANTIPORTER 1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46157">
        <property type="entry name" value="K(+) EFFLUX ANTIPORTER 3, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02254">
        <property type="entry name" value="TrkA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0050">Antiport</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0630">Potassium</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="610"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="638"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="664"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="693"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="722"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="754"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="797"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="838"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="910"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="934"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="970"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1132"/>
            <end position="1153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1160"/>
            <end position="1178"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="12">
        <location>
            <begin position="1018"/>
            <end position="1135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="250"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="371"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="258"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="374"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="456"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9681552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1239" mass="134922" checksum="4C0F0CACBB889CF1" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z3U7</accession>
    <name>A0AA38Z3U7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Formin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_018001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the formin-like family. Class-I subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9681900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z3U7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045010">
        <property type="term" value="P:actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2220">
        <property type="entry name" value="Formin, FH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015425">
        <property type="entry name" value="FH2_Formin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042201">
        <property type="entry name" value="FH2_Formin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027643">
        <property type="entry name" value="Formin-like_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23213:SF177">
        <property type="entry name" value="FORMIN-LIKE PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23213">
        <property type="entry name" value="FORMIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02181">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00498">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51444">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="13"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="174"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="FH2" evidence="5">
        <location>
            <begin position="553"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="112"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="254"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="385"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="144"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="254"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="391"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="434"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="461"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="482"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="497"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="508"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00025793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9681900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="974" mass="106257" checksum="A22F1B3CD11D5E79" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z466</accession>
    <name>A0AA38Z466_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Sodium/hydrogen exchanger</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_018119</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>K(+)(in) + H(+)(out) = K(+)(out) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:29467"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>Na(+)(in) + H(+)(out) = Na(+)(out) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:29419"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z466"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015386">
        <property type="term" value="F:potassium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015385">
        <property type="term" value="F:sodium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098719">
        <property type="term" value="P:sodium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018422">
        <property type="entry name" value="Cation/H_exchanger_CPA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004709">
        <property type="entry name" value="NaH_exchanger"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00840">
        <property type="entry name" value="b_cpa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110">
        <property type="entry name" value="SODIUM/HYDROGEN EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110:SF197">
        <property type="entry name" value="SODIUM_HYDROGEN EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01084">
        <property type="entry name" value="NAHEXCHNGR"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0050">Antiport</keyword>
    <keyword evidence="8 13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0630">Potassium</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="10 13" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="4 13 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 13" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="25"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="57"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="86"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="115"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="220"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="263"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="308"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="340"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="386"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="419"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="15">
        <location>
            <begin position="46"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9682080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="541" mass="59426" checksum="4BF6D2DE8F7A466C" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z4J8</accession>
    <name>A0AA38Z4J8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">S-acyltransferase</fullName>
            <ecNumber evidence="8">2.3.1.225</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Palmitoyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_018167</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="8">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="8"/>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z4J8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF286">
        <property type="entry name" value="PROTEIN S-ACYLTRANSFERASE 17-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="75"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="110"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="200"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="265"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="9">
        <location>
            <begin position="154"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9682145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="409" mass="46704" checksum="E3B537B27B2C2AE7" modified="2024-01-24" version="1">MAVQWLLVCHGLATLLVVVSFLCGQWPIFQGTFIERIHYFITFGAYDYFLGVFGSKGTNVILSVENFCCDRPNPILQVVYLAIIGATYYFIVQSTFSYIPGYYISGVHRYTSLLAVGVGVVLFLLTSFSDPGTVTAENVSKYLSAYPYDNIIYSEKECATCRIPKPARSKHCSICDRCVARFDHHCGWMNNCIGERNTRFFMAFLLWHFLLCIYGTVAIGLVLAGRLKELKVIHILTVYYGIENSFASLAPHVVQWLLGSYNTQLLLMVFLAIVSLLLAGFFSYHANLCFTNTTTNETFKWEDYIRWQQKLNEAKASTAALKAGISDLNCERKPPESKWKAFFRRSRLEDVEPVSKNNIYDKGFLRNLHEIIFPSHPDIPFHKPNQSLAKNLDKLDSLFIFIYGYYMVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2025-10-08" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z521</accession>
    <name>A0AA38Z521_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_018450</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057135">
        <property type="entry name" value="At4g27190-like_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050905">
        <property type="entry name" value="Plant_NBS-LRR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463:SF198">
        <property type="entry name" value="RPP4C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23247">
        <property type="entry name" value="LRR_RPS2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="167"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="1327"/>
            <end position="1464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9682531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="2232" mass="256054" checksum="D70931E967368EA6" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z6D2</accession>
    <name>A0AA38Z6D2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Polysaccharide biosynthesis domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_019002</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z6D2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045492">
        <property type="term" value="P:xylan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006514">
        <property type="entry name" value="IRX15/GXM/AGM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01627">
        <property type="entry name" value="A_thal_3515"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31444">
        <property type="entry name" value="OS11G0490100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21729">
        <property type="entry name" value="IRX15_IRX15L_GXM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9683241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="300" mass="33638" checksum="46FBE3420B6D3404" modified="2024-01-24" version="1">MKNRRVPFERPWLLTAVIAGMICAAILINSFGRTSSNSLLCSLSGAYTRPGHSDDSVAQTQLIAILHYATSRVVPQQSLAEIRVSFDVLQSLAPCNFLVYGLGHDSVMWSSFNPKGTTIFLEEDPKWVQTVLKQAPNLLAHTVRYRTHLSEADQLLGSYKSEPACLPPDVRLRDNTACRLALTGLPQEVYDTEWDLIMIDAPRGYFPEAPGRMGAIFTAAVMARARKRQGVTHVFLHDVNRRIEKVYAEEFLCKKNLVKAVGRLWHFAIPSTANDTMTSALLLKMFLTKSSTFLFLFFLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZBY5</accession>
    <name>A0AA38ZBY5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF455 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_015200</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZBY5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00657">
        <property type="entry name" value="Ferritin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007402">
        <property type="entry name" value="DUF455"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42782:SF4">
        <property type="entry name" value="DUF455 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42782">
        <property type="entry name" value="SI:CH73-314G15.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04305">
        <property type="entry name" value="DUF455"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9686190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="381" mass="42082" checksum="ECF5923487E9CEAD" modified="2024-01-24" version="1">MLSTTLRCAWCSSLFPPLSFSTKPLSSSSSSSSSLHYSPWSGLQSWRENPLNKYRLWGPNGPEPLPPSPCTPLEVCEGPMALASSLAEMGSLVLSTSDPLTKSKLSHLAYSRWRTEHLPIGDSQAPYRPARPAKPQLVSPKEIPAPKNSGLPLNAYMLHNLAHVELNAIDLAWDTVVRFSPYSKILGEGFFADFAHVADDESRHFAWCSQRLAELGFNYGDMPAHNLLWRECAKSSNNVAARLVAIPLVQEARGLDAGPRLVQKLIGFGDSRTSNIVARIADEEVAHVAVGVHWFVSVCQKMARAPCSTFKDLLKEYNVELKGPFNYSARDEAGIPRDWYDTSSTPEVKQDKSEPLSQVYERLACIISMENENSSLNRPPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZC49</accession>
    <name>A0AA38ZC49_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="9">Methylthioribulose-1-phosphate dehydratase</fullName>
                <shortName evidence="9">MTRu-1-P dehydratase</shortName>
                <ecNumber evidence="9">4.2.1.109</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="9">Enolase-phosphatase E1</fullName>
                <ecNumber evidence="9">3.1.3.77</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="9">2,3-diketo-5-methylthio-1-phosphopentane phosphatase</fullName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_015275</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>5-(methylsulfanyl)-D-ribulose 1-phosphate = 5-methylsulfanyl-2,3-dioxopentyl phosphate + H2O</text>
            <dbReference type="Rhea" id="RHEA:15549"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58548"/>
            <dbReference type="ChEBI" id="CHEBI:58828"/>
            <dbReference type="EC" id="4.2.1.109"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>5-methylsulfanyl-2,3-dioxopentyl phosphate + H2O = 1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:49252"/>
            <dbReference type="ChEBI" id="CHEBI:58828"/>
            <dbReference type="EC" id="3.1.3.77"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="9">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 2/6.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 3/6.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 4/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">In the C-terminal section; belongs to the HAD-like hydrolase superfamily. MasA/MtnC family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">In the N-terminal section; belongs to the aldolase class II family. MtnB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.109" evidence="9"/>
    <dbReference type="EC" id="3.1.3.77" evidence="9"/>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZC49"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043874">
        <property type="term" value="F:acireductone synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046570">
        <property type="term" value="F:methylthioribulose 1-phosphate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019509">
        <property type="term" value="P:L-methionine salvage from methylthioadenosine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01629">
        <property type="entry name" value="HAD_EP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.720.60:FF:000001">
        <property type="entry name" value="Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.225.10:FF:000010">
        <property type="entry name" value="Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000088">
        <property type="entry name" value="Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="entry name" value="Class II aldolase/adducin N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03116">
        <property type="entry name" value="Salvage_MtnB_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03118">
        <property type="entry name" value="Salvage_MtnBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001303">
        <property type="entry name" value="Aldolase_II/adducin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023943">
        <property type="entry name" value="Enolase-ppase_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017714">
        <property type="entry name" value="MethylthioRu-1-P_deHdtase_MtnB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027505">
        <property type="entry name" value="MtnB_viridiplantae"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027514">
        <property type="entry name" value="Salvage_MtnB_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01691">
        <property type="entry name" value="enolase-ppase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03328">
        <property type="entry name" value="salvage_mtnB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20371">
        <property type="entry name" value="ENOLASE-PHOSPHATASE E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20371:SF1">
        <property type="entry name" value="ENOLASE-PHOSPHATASE E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00596">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01133">
        <property type="entry name" value="C1.5.4:_Enolase-phosphatase_Li"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00044">
        <property type="entry name" value="enolase-phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01007">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="AraD/HMP-PK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 9" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="8 9" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Class II aldolase/adducin N-terminal" evidence="10">
        <location>
            <begin position="229"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Methylthioribulose-1-phosphate dehydratase" evidence="9">
        <location>
            <begin position="1"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Enolase-phosphatase E1" evidence="9">
        <location>
            <begin position="484"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor; for methylthioribulose-1-phosphate dehydratase activity" evidence="9">
        <location>
            <position position="355"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="312"/>
        </location>
        <ligand>
            <name>substrate</name>
            <label>1</label>
            <note>for methylthioribulose-1-phosphate dehydratase activity</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="332"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="405"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="487"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="489"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="622"/>
            <end position="623"/>
        </location>
        <ligand>
            <name>substrate</name>
            <label>2</label>
            <note>for enolase-phosphatase activity</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="656"/>
        </location>
        <ligand>
            <name>substrate</name>
            <label>2</label>
            <note>for enolase-phosphatase activity</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="682"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9686298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="723" mass="80732" checksum="EBE646D7542D3866" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZFV4</accession>
    <name>A0AA38ZFV4_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_014148</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZFV4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48205">
        <property type="entry name" value="OS01G0742766 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48205:SF1">
        <property type="entry name" value="OS01G0742766 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9688308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="98" mass="10712" checksum="094C6CCAD7D82B3D" modified="2024-01-24" version="1">MPEMEPESKKRRIGHAISRRMVSSPAAKNDVVSTSTDIPTVPLPAAHPGGIHFRIEQAKRHAVAQAQQEGCTANFKIFDSRFGNFLLPVIPTHAELNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZFY8</accession>
    <name>A0AA38ZFY8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">CRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_014058</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZFY8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000373">
        <property type="term" value="P:Group II intron splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.110.60:FF:000003">
        <property type="entry name" value="CRM-domain containing factor CFM3B, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.110.60:FF:000002">
        <property type="entry name" value="CRS2-associated factor 1, chloroplastic"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.60">
        <property type="entry name" value="YhbY-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045278">
        <property type="entry name" value="CRS1/CFM2/CFM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001890">
        <property type="entry name" value="RNA-binding_CRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035920">
        <property type="entry name" value="YhbY-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31846">
        <property type="entry name" value="CRS1 / YHBY (CRM) DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31846:SF7">
        <property type="entry name" value="CRS1 _ YHBY (CRM) DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01985">
        <property type="entry name" value="CRS1_YhbY"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01103">
        <property type="entry name" value="CRS1_YhbY"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75471">
        <property type="entry name" value="YhbY-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51295">
        <property type="entry name" value="CRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="3" id="KW-0934">Plastid</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="CRM" evidence="11">
        <location>
            <begin position="265"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="CRM" evidence="11">
        <location>
            <begin position="468"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="domain" description="CRM" evidence="11">
        <location>
            <begin position="681"/>
            <end position="781"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9688129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="884" mass="100174" checksum="C61DF03D4CA637B5" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZG49</accession>
    <name>A0AA38ZG49_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_014228</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZG49"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14798">
        <property type="entry name" value="RX-CC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038005">
        <property type="entry name" value="RX-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF1238">
        <property type="entry name" value="TOMV SUSCEPTIBLE PROTEIN TM-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="6">
        <location>
            <begin position="10"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="5">
        <location>
            <begin position="168"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="7">
        <location>
            <begin position="419"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="8">
        <location>
            <begin position="563"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="912"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="912"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="945"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="958"/>
            <end position="967"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9688455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="967" mass="109698" checksum="18E9B8DDFDCBE55F" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZGA6</accession>
    <name>A0AA38ZGA6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Oxidative stress 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_014140</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZGA6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051992">
        <property type="entry name" value="OxStress_Response_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33172">
        <property type="entry name" value="OS08G0516900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33172:SF103">
        <property type="entry name" value="PROTEIN OXIDATIVE STRESS 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="46"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="54"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9688295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="184" mass="19836" checksum="228A1FD832689A98" modified="2024-01-24" version="1">MGETAKQSFDDLGFKGIDANGTHQHNQWLIMGGADDNSDTISMEFSSFEDSDNSLESSSSLDLTEDASSSTSPLSNGPLYELSELMAHLPIKRGLSKYFQGKSQSFTSLASVKSLEDLAKKGTPSRKKMKSCKSYGGGLDLHKLYTPKATISKKASRGSLLSSLGRRGVIMGSCRPPSPLQKNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZIB1</accession>
    <name>A0AA38ZIB1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">WRKY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_014857</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9689375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZIB1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.80:FF:000004">
        <property type="entry name" value="WRKY transcription factor 65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.80">
        <property type="entry name" value="WRKY domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003657">
        <property type="entry name" value="WRKY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036576">
        <property type="entry name" value="WRKY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044810">
        <property type="entry name" value="WRKY_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018872">
        <property type="entry name" value="Zn-cluster-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31282">
        <property type="entry name" value="WRKY TRANSCRIPTION FACTOR 21-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10533">
        <property type="entry name" value="Plant_zn_clust"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03106">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00774">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118290">
        <property type="entry name" value="WRKY DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50811">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="WRKY" evidence="7">
        <location>
            <begin position="219"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="76"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="157"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="81"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="122"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9689375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="297" mass="32256" checksum="870EDEFF75296A7C" modified="2024-01-24" version="1">MAVDLLGFSKMDEQMAIQEAASAGLKSMEHLILLLNHHQPQSQQINHLDCREITDFTVSKFKQVISILNRTGHARFRRGPVTSSPSLSPYDMSNKSELPKPVESSPFHSNLVLSAKPDPLKSEGNASVSSTTSSFLSSITGDGSVSNGKLGTSLFVPPPAPAVSAGKPPLSSSQRRKCHEHGSSDNISGKLSVSGRCHCSKRRKNRVKRTIRVPAISSKIADIPADEYSWRKYGQKPIKGSPYPRGYYKCSSVRGCPARKHVERAPDDPAMLIVTYEGEHRHSQTPAPAGGLLFAST</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZP53</accession>
    <name>A0AA38ZP53_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SNRNP25 ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_011674</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZP53"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17058">
        <property type="entry name" value="Ubl_SNRNP25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039690">
        <property type="entry name" value="SNRNP25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040610">
        <property type="entry name" value="SNRNP25_ubiquitin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14942:SF9">
        <property type="entry name" value="OS02G0188500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14942">
        <property type="entry name" value="U11/U12 SMALL NUCLEAR RIBONUCLEOPROTEIN 25 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18036">
        <property type="entry name" value="Ubiquitin_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SNRNP25 ubiquitin-like" evidence="1">
        <location>
            <begin position="50"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9692703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="233" mass="25983" checksum="91F681E846167903" modified="2024-01-24" version="1">MRSGGEVGRRRGGCDSGGGGGCSVLCRSLSSLLPIDGNLSYRKLSLDLYKLSILKLDGTSFDVQIARNAKVAELKQAIEEVFSLSTNEGEENIISWSHVWGHFCLCYEGQKLVNDKAHIRDFGIQDGDQLQFIQHITIDRNSKKKRPKKESVACRQSQMLFAGSRQRDGDGTEDEEEIPMPEFKLAHFLKGWLPYSRARSMGRRGSEGRIYSPRFGGRCLGVRCRPGIIRLGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZPB1</accession>
    <name>A0AA38ZPB1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Disease resistance protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_011379</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZPB1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050905">
        <property type="entry name" value="Plant_NBS-LRR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463:SF180">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RPS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0611">Plant defense</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9692292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="148" mass="16711" checksum="4F0C7F51CC1FA32C" modified="2024-01-24" version="1">MKLDLEKAGEWGFAADGILNSNFQCLCHVIISQLPKLLHLTCLIYIPSLEFLGVTKCESMEELIGEEDSKGVILDDLPNLRSISEQALPFPSLTEIAVYNCPKLRKLPLDSNSATNSLKKIKGESSWWEELQWKDETLKSTFTPYFDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZPN3</accession>
    <name>A0AA38ZPN3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PIFI-like Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_011667</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast stroma</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZPN3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009570">
        <property type="term" value="C:chloroplast stroma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009579">
        <property type="term" value="C:thylakoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010478">
        <property type="term" value="P:chlororespiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057612">
        <property type="entry name" value="Ig_PIFI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044960">
        <property type="entry name" value="RCA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32429">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32429:SF9">
        <property type="entry name" value="PROTEIN POST-ILLUMINATION CHLOROPHYLL FLUORESCENCE INCREASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25419">
        <property type="entry name" value="Ig_PIFI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIFI-like Ig-like" evidence="2">
        <location>
            <begin position="83"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9692689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="282" mass="30397" checksum="469C99A2801BCA05" modified="2024-01-24" version="1">MAATTGSIFASSTQCFPTVTSVSGTNGSPSVAGRLVSNFMGASLRSRLPKMGKVVKVNGKVSAAAVTTTPVQETKEVKLPTWAMFELGRAPVYWKTMNGLPPSSGEKLKIFYNPAASKLVPNEDFGIGFNGGFNQPIMCGGEPRAMLQKTRGKADRPIYTIQICIPKHAVNLIFSFTNGTEWDGPYKLLFQVPKAWRNKPIEFFNQGLAEELSKEGACDKAIFPDTNIVIDRCALIGNLTLEGGDRCNLDFVIGCTDPSSPLYNPLANVDDGSCPIESDSED</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZPW0</accession>
    <name>A0AA38ZPW0_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_012002</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9693090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZPW0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9693090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="117" mass="12688" checksum="B511423828F7AADC" modified="2024-01-24" version="1">MSGGVVGEAGLVMSEVEGSGYEEKGYGGGYTQGNSCTWKVEGNELKVLKATEVVVLDDTKELCSSRGADVRDYSGIDLQENHIILSALGEVSIVTQVLQVVHARVRLSVMTRYFRPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZVG4</accession>
    <name>A0AA38ZVG4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_010844</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZVG4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009570">
        <property type="term" value="C:chloroplast stroma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02947">
        <property type="entry name" value="TRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000245">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044253">
        <property type="entry name" value="WCRKC1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47192:SF3">
        <property type="entry name" value="THIOREDOXIN-LIKE 3-1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47192">
        <property type="entry name" value="THIOREDOXIN-LIKE 3-2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0249">Electron transport</keyword>
    <keyword evidence="3" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Thioredoxin" evidence="4">
        <location>
            <begin position="56"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9695562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="185" mass="21365" checksum="85670E9DD3542D4C" modified="2024-01-24" version="1">MSILTANTHIVYREVLLRDPQNQLSGGGSCLFWSRSPGFGVDGRGGEWRKTKKRDLRVEAFWADMTRPNVIEMQPIKDCDQLDQILAQAQELHQPIIIDWMAAWCRKCIYLKPKLEKLATEYDTKIKFYSVDVNNVSQALVKRGNITKMPTIQLWKDGEMKAEVIGGHKAWIVIDEVREMIQKFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZVJ6</accession>
    <name>A0AA38ZVJ6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_011026</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the AP2/ERF transcription factor family. AP2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZVJ6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000004">
        <property type="entry name" value="AP2-like ethylene-responsive transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32467">
        <property type="entry name" value="AP2-LIKE ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32467:SF32">
        <property type="entry name" value="AP2-LIKE ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR SMOS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00367">
        <property type="entry name" value="ETHRSPELEMNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="9">
        <location>
            <begin position="70"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="235"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="274"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="308"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="308"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="324"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9695839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="429" mass="47370" checksum="823F1C89E55A1738" modified="2024-01-24" version="1">MASSSSEPGLKPESGGGSGSGGGETSEAAVASDQLLLYRGLKKAKKERGSTAKERISKMPPCAAGKRSSIYRGVTRHRWTGRYEAHLWDKSTWNQNQNKKGKQVYLGAYDDEEAAARAYDLAALKYWGPGTLINFPVTDYARDLEEMQNVSREEYLASLRRKSSGFSRGISKYRGLASNRWDQPFGRIAGQEYFNNMHYGMGDDAAAESEFFGGFCMERKIDLTGYIKWWGPNKTRQSDSLAKSSEETKQGSGEDIGSELKTLEWAIQPTEPYQMPRLGLPHEGKKQKSSTVSALSILSRSAAYKSLEEKASKKQENNTDNDENENKNKINKMDHGKAVEKSTSHDSGSERLGVALGLGGEFSIQRSVYPLTPLLSAPLLTNYSSVDPLADPILWTSLVPALPTGLPRTAEVTKTETSSTYTLFHQEEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZWR4</accession>
    <name>A0AA38ZWR4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">gibberellin 2beta-dioxygenase</fullName>
            <ecNumber evidence="9">1.14.11.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_008775</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>gibberellin A1 + 2-oxoglutarate + O2 = gibberellin A8 + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:15005"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:58524"/>
            <dbReference type="ChEBI" id="CHEBI:58594"/>
            <dbReference type="EC" id="1.14.11.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Hormone biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Plant hormone biosynthesis; gibberellin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the iron/ascorbate-dependent oxidoreductase family. GA2OX subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.13" evidence="9"/>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZWR4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045543">
        <property type="term" value="F:gibberellin 2-beta-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009685">
        <property type="term" value="P:gibberellin metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.330:FF:000025">
        <property type="entry name" value="Gibberellin 2-beta-dioxygenase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050231">
        <property type="entry name" value="Iron_ascorbate_oxido_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47990">
        <property type="entry name" value="2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00682">
        <property type="entry name" value="IPNSYNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="5 10" id="KW-0408">Iron</keyword>
    <keyword evidence="2 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="11">
        <location>
            <begin position="168"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00052204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00066708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9696726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="333" mass="37073" checksum="8DDAE120B12B1386" modified="2024-01-24" version="1">MVVPSPSPIRSKKTKAVGIPVIDLSLNRSAIAELIVNACEDYGFFKVVNHGVPKEIIGRLEEEGLSFFAKPSSEKQKAGPASPFGYGCKNIGFNGDRGELEYLLLHTNPVSISERSKAISNDPTEFSCAVNDYIQGVRELCCEILDLMGEGLWLQDKMVFSRMIRDVHSDSVIRVNHYPAVKDVKEWDPCDPIGFGEHSDPQILTILRSNDVPGLQIRLRDGLWVPVPPDPTEFCVFVGDALEAMTNGRLMSVRHRALTSSVKARLSMMYFGAPPLNAWISPLPDMVSPQKPSLFRPFSWVEYKKAAYSLRLGDRRLDLFKTNTSSTGDKVEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZWW1</accession>
    <name>A0AA38ZWW1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Coenzyme Q-binding protein COQ10 START domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_008820</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZWW1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd08866">
        <property type="entry name" value="SRPBCC_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005031">
        <property type="entry name" value="COQ10_START"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34060">
        <property type="entry name" value="POLYKETIDE CYCLASE / DEHYDRASE AND LIPID TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34060:SF1">
        <property type="entry name" value="POLYKETIDE CYCLASE _ DEHYDRASE AND LIPID TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03364">
        <property type="entry name" value="Polyketide_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Coenzyme Q-binding protein COQ10 START" evidence="1">
        <location>
            <begin position="109"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9696798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="274" mass="30646" checksum="F6020ED5F2CEC19C" modified="2024-01-24" version="1">MRAFPISSGTPHTLFFRGGGTSPGAAAAANRIRSPSSTSSSHALVGAAASPKLFSYKFGYSNEDSTSFRGEGDGDEEIEVEKLENNGVEEVEIEKLGNNSRRIRSKIVIDANLHTVWSILTDYEGLADFIPGLAVSQLVEKREKFARLFQIGQQDLAFGLKFNAKGIVDCYEKDLESLPSGQKRDIEFKMIEGDFQIFEGKWSIEQRNTNTWKGKDSSVGQEFYTTLTYVVDVEPKLWLPVYLVEGRLSREIKMNLTCIREEAKKRTRNPLSAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZZG0</accession>
    <name>A0AA38ZZG0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_006990</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9697647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZZG0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36617">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36617:SF15">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase zinc-binding" evidence="1">
        <location>
            <begin position="141"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9697647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="325" mass="38142" checksum="9D2375600C1C63D6" modified="2024-01-24" version="1">MDFIEDLGNMGGWCSKGVRDCYGVGVWKAIRNGWENFRSHSRFSVGDGTRVKFWKDLWCENQTLEEAFPTLFNVSVNKEGWVAEAWEEDGVGGNWGLRFNRHLNDWEVGEVESLLGKLHPLSIRRGVDDLLRWKENRNGTFSVKSSYSSLSRGIKPPFPARTIWTPWVPIRASFFGWEAAWSRLLTTDCLKRFGWSIPNRCFLCKNEEETTDHLLLFCEKARMLWLLIFSLFGVQWVMHSSVKRNLLGWHGSFVGKKREKTWRVAPLCLMWTIWKQRNRRAFDGAKRNNQDIKSIFLYTFVNWARVYIVEHTLSLIDFVDWLATK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZZW8</accession>
    <name>A0AA38ZZW8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GAG-pre-integrase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_009284</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZZW8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029472">
        <property type="entry name" value="Copia-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025724">
        <property type="entry name" value="GAG-pre-integrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054722">
        <property type="entry name" value="PolX-like_BBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34222:SF40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34222">
        <property type="entry name" value="GAG_PRE-INTEGRS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13976">
        <property type="entry name" value="gag_pre-integrs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22936">
        <property type="entry name" value="Pol_BBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14244">
        <property type="entry name" value="Retrotran_gag_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Retrotransposon Copia-like N-terminal" evidence="2">
        <location>
            <begin position="32"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="Retrovirus-related Pol polyprotein from transposon TNT 1-94-like beta-barrel" evidence="3">
        <location>
            <begin position="355"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="GAG-pre-integrase" evidence="1">
        <location>
            <begin position="461"/>
            <end position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9697393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="589" mass="66641" checksum="AA9F60EC8DE04D63" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A077</accession>
    <name>A0AA39A077_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DDE Tnp4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_007428</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HARBI1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A077"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045249">
        <property type="entry name" value="HARBI1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930:SF291">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE Tnp4" evidence="9">
        <location>
            <begin position="166"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9698344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="384" mass="43841" checksum="E31BD7725D2C5243" modified="2024-01-24" version="1">MGPVRGYKKRRKIEKREESSEEGSVDWWDEFSKRIAGLLSSSKGLDKFESVFKISRKTFNYICALVKEDMMAKPGNFIFTNGRPMSLNDQVAVALRRLSSGDSLLTIGDAFGLNHSTVSQVTWRFVEIMEERALHHLQWPSTEPEITEITSKFEKIRGLPNCCGAIDTTHIMMCLPSADSANSVWLDSENHHSMILQAIVDPEMRFRDIVTGWPGKMKDSSVLQSSNFFKLCEKGQRLNGKKIELAEGSEISEYIVGDSGYPLLPWLVTPYQGKELPESKAEFNRRHFATRMVAQRALARLKEMWKVIQGVMWRPDKNRLPRIILVCCLLHNIVIDLEDEVQDEMPLSHHHDLGYRQQICESADNNASIVRDKLSLYLSGRLPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A0L2</accession>
    <name>A0AA39A0L2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Tetraspanin-3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_007350</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A0L2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044991">
        <property type="entry name" value="TET_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32191">
        <property type="entry name" value="TETRASPANIN-8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="74"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="238"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9698217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="274" mass="30688" checksum="01196E1B3814BD0E" modified="2024-01-24" version="1">MRASNHLIGLLNFLTFLLSIPILGGGIWLSSRANNTDCMKFLQWPLIIIGVAIMVVSLAGFAGACYRNTFLMWFYLWAMFFIIAALVGFVIFAYAVTDKGSGRAVSNRVYLDYYLQDYSGWLQERVSEDSYWRKISSCVRDSKVCAKMGRNIGGVPESADLFYLRKLNPIESGCCKPPTECGYVYVNETVWNTGGGMVVSNSDCNNWSNDQGQLCYSCNSCKAGVLASLKKSWRKVSVINIVILIILVIVYVVACAAFRNNRRSIMMRHMVKQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A0R0</accession>
    <name>A0AA39A0R0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein LURP-one-related 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_007755</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LOR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A0R0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.200">
        <property type="entry name" value="LURP1-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007612">
        <property type="entry name" value="LOR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038595">
        <property type="entry name" value="LOR_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025659">
        <property type="entry name" value="Tubby-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31087">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31087:SF85">
        <property type="entry name" value="PROTEIN LURP-ONE-RELATED 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04525">
        <property type="entry name" value="LOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54518">
        <property type="entry name" value="Tubby C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9698826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="186" mass="20595" checksum="64FD9CF4D105A064" modified="2024-01-24" version="1">MASSAQAYPSDFQIPFDLFISKKHPGLPRGYLGLADSSGNIVYRVNRRSPKHNRVLLDAAGNPLISMRGSHNGSWQCFRGDGSGEDLIFRVERTLNTLTRTEFNIFLGGANCEDSTDFKMKGCPFQRSCTIYKGDSIVAQTSLMYKLGKAFVGRCKFRLTIFPGSGDHAVVVALIVIFFDGRRLSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A0W4</accession>
    <name>A0AA39A0W4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Exocyst subunit Exo70 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_007723</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Component of the exocyst complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the EXO70 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A0W4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.170:FF:000003">
        <property type="entry name" value="Exocyst subunit Exo70 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.170">
        <property type="entry name" value="Exocyst complex component Exo70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004140">
        <property type="entry name" value="Exo70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046364">
        <property type="entry name" value="Exo70_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12542:SF96">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT EXO70B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12542">
        <property type="entry name" value="EXOCYST COMPLEX PROTEIN EXO70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03081">
        <property type="entry name" value="Exo70_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20669">
        <property type="entry name" value="Exo70_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex subunit Exo70 C-terminal" evidence="5">
        <location>
            <begin position="268"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="148"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU365026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9698792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="626" mass="70939" checksum="CF4C8BFB47CBDDC5" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A147</accession>
    <name>A0AA39A147_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_007542</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A147"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9698517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="122" mass="13600" checksum="E60C5DDB7CCC40D7" modified="2024-01-24" version="1">MMTTPWLPLHGPPRAEREGTLSDSQRPTKPPIPPPFPSPEAPMPLPVAVPTRTNRILKRMPHMQISWRVPADTARFVEAMNEGPMEFRMFILLGYSNQKMGPLAHAEDAYLTLMQSNGGGVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A2F2</accession>
    <name>A0AA39A2F2_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_005536</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9699711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A2F2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37702">
        <property type="entry name" value="PROLINE-RICH FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37702:SF9">
        <property type="entry name" value="PROLINE-RICH FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041260891" evidence="2">
        <location>
            <begin position="26"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9699711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="181" mass="19545" checksum="EA286D8637AB07AF" modified="2024-01-24" version="1">MERPRRVRMLLFVAVFMVVQRQSWPATADVAAKSKYQIECTMCSACDNPCNQVPSPPPPNPSPPPPSPPPPSSSSNCPPPPSPPSNPTYYYSPPPPSQPTYVYSSPPPPNGYNGGGGGGGGGAFYPPPYQNYPAPPPPNPMLPYFPFWYHTPPPKSAANSVYFSDLTVYTIALISLLFCFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A3L9</accession>
    <name>A0AA39A3L9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_005915</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A3L9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13983">
        <property type="entry name" value="STKc_WNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000075">
        <property type="entry name" value="Probable serine/threonine-protein kinase WNK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000046">
        <property type="entry name" value="probable serine/threonine-protein kinase WNK9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024678">
        <property type="entry name" value="Kinase_OSR1/WNK_CCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050588">
        <property type="entry name" value="WNK_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13902">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE WNK WITH NO LYSINE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12202">
        <property type="entry name" value="OSR1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="24"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="538"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="619"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="544"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="640"/>
            <end position="649"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9700353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="731" mass="83952" checksum="1D5BE5A6CA3D93E0" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A4L4</accession>
    <name>A0AA39A4L4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">3'-5' exonuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_005617</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9699838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A4L4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06141">
        <property type="entry name" value="WRN_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051132">
        <property type="entry name" value="3-5_Exonuclease_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13620">
        <property type="entry name" value="3-5 EXONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13620:SF59">
        <property type="entry name" value="POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9699838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="217" mass="25199" checksum="5E33C08E45E67513" modified="2024-01-24" version="1">MAGTRMAIIERASGSEKYRMFSVVVHSTRITVTVTFNASVVRKWIRSALYFYRYQLNRLVVGLGVQWVPDFIRGEDNPAATLQLCIGRRCLVFQLLHSNGVPLLLRRFLLNPRYVFVGIWNHRDAYKLSRSRHALEVHRLVDLRHYAVTTRGEGLATKSMEEIAKEYLGFEGVGLEKSVSLSQWDEVRLSREQVWQACYDVFVCFQVGRKTKIWKKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A4L8</accession>
    <name>A0AA39A4L8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glutathione S-transferase</fullName>
            <ecNumber evidence="3">2.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_006286</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Is involved in the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GST superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A4L8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03185">
        <property type="entry name" value="GST_C_Tau"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03058">
        <property type="entry name" value="GST_N_Tau"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000012">
        <property type="entry name" value="Tau class glutathione S-transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000014">
        <property type="entry name" value="Tau class glutathione S-transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045074">
        <property type="entry name" value="GST_C_Tau"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045073">
        <property type="entry name" value="Omega/Tau-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11260:SF775">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE U10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11260">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE, GST, SUPERFAMILY, GST DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13410">
        <property type="entry name" value="GST_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01152">
        <property type="entry name" value="Main.3:_Omega-_and_Tau-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="4">
        <location>
            <begin position="2"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="5">
        <location>
            <begin position="86"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU369102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9700878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="224" mass="25362" checksum="B28275DEB08FB537" modified="2024-01-24" version="1">MAEVKLLGFWASPFSDRVIWALKLKGINYEYVEEDPYNKTQLLLQHNPVHKKVPVLIHAGKPIAESSMILEYIEETWPQNPLLPQGAYEKAMAHFWIKFGEEKMATICAVLRTAGAEQERAIKEAMELLKILEEQGLGDKKFFGGDAIGLADIAFGWLAYLFGVIEEVAGVKVLDANRLPRLHAWIDNLKEVPVIKENLPDGQKLLGHLKRRRETHLALPNCNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A4M0</accession>
    <name>A0AA39A4M0_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_005936</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ERG2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A4M0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006716">
        <property type="entry name" value="ERG2_sigma1_rcpt-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10868">
        <property type="entry name" value="SIGMA 1-TYPE OPIOID RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10868:SF1">
        <property type="entry name" value="SIGMA NON-OPIOID INTRACELLULAR RECEPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04622">
        <property type="entry name" value="ERG2_Sigma1R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="125"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="7"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9700377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="354" mass="39896" checksum="A83A8200D96F6E1D" modified="2024-01-24" version="1">MKTVLPTSSTAKSSTTTTTMAEERSETRDSCYFPGCRKDANCNCEMCLDSISATLDLLPASIQRSTLTKLSAPKTVERTPVSFNSSVMSTPESGTSKTSLSPAVRSTARVSLEQKKKKRKRVCRVGLYFWRLVVGVGLIFAAEFGFSCGVSRVLRPELSWDIVRSVGERSWVVKDLNEKVGFLQKELQGFVGGEVSKCSYIDSTWEVSQDGLLLNSRCTLYKSVTEEVTIWGWPLQTAGLLATGLSSRSFTILSGRVTEWPDGRIRNLLRKANSSWTHKKWSASVVQLDPNTLVLEYRRSSILENSRLFSAALQFLRFWMCRMVERMKQEFWVLSVFKIKYTDFTAKESDRIPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A4Q7</accession>
    <name>A0AA39A4Q7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">GATA-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_006312</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the type IV zinc-finger family. Class A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A4Q7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000018">
        <property type="entry name" value="GATA transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051140">
        <property type="entry name" value="GATA_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45658">
        <property type="entry name" value="GATA TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45658:SF42">
        <property type="entry name" value="GATA TRANSCRIPTION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00344">
        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0010">Activator</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041396484" description="GATA-type domain-containing protein" evidence="12">
        <location>
            <begin position="17"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="GATA-type" evidence="13">
        <location>
            <begin position="177"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="94"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="101"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9700920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="258" mass="29422" checksum="43166CD158F59785" modified="2024-01-24" version="1">MRFLFLFLEILNVEMATIFIALSCQSQNQSCTNKCARRFFLFQTFLRPTISIFLLLDAPVEEELEWLNKDVFPGVETFLDYLPTTVENIPKQQSPISVLENSSHSSSSNNSNSSTTTIMSCCENLRVPSRARSKRRRRRHKGFSDIPGQPWWWWSSQGNTNANNNSTTNSKQTITSPTIGRKCQHCQAEKTPQWRAGPLGPKTLCNACGVRYKSGRLVPEYRPASSPTFSSKVHSNSHRKIMEMRKLKQRDVVVRPCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A541</accession>
    <name>A0AA39A541_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CASP-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_005778</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3 8">Homodimer and heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Casparian strip membrane proteins (CASP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A541"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006459">
        <property type="entry name" value="CASP/CASPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006702">
        <property type="entry name" value="CASP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044173">
        <property type="entry name" value="CASPL"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01569">
        <property type="entry name" value="A_tha_TIGR01569"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36488">
        <property type="entry name" value="CASP-LIKE PROTEIN 1U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36488:SF8">
        <property type="entry name" value="CASP-LIKE PROTEIN 1U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04535">
        <property type="entry name" value="CASP_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="90"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="129"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="181"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="Casparian strip membrane protein" evidence="10">
        <location>
            <begin position="40"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="9"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9700108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="208" mass="21778" checksum="BE41AA0F7F70FEE9" modified="2024-01-24" version="1">MSSADIEKPAPPPLETEAPPPPPPPLPPPPPPPPPPADYTALDVVLRILLLLSTVAAVVVMVTGVETKLVSVPGVPVLVPNKAKFQNSPALIYFVAALSVVGLYSIISTLGSFLLIAKPSCSTKAILHLAIWDVLMLGLAASATGTAGGVAYIGLKGNSHVGWTKVCNVYGKFCRHVGSSVAVALFASILLVLLIWLSLYTLYSRIRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A7P3</accession>
    <name>A0AA39A7P3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ADP/ATP translocase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">ADP,ATP carrier protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_003963</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the exchange of ADP and ATP across the membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ADP(in) + ATP(out) = ADP(out) + ATP(in)</text>
            <dbReference type="Rhea" id="RHEA:34999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:35000"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9701982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A7P3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005471">
        <property type="term" value="F:ATP:ADP antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140021">
        <property type="term" value="P:mitochondrial ADP transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990544">
        <property type="term" value="P:mitochondrial ATP transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000071">
        <property type="entry name" value="probable ADP,ATP carrier protein At5g56450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002113">
        <property type="entry name" value="ADT_euk_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45635">
        <property type="entry name" value="ADP,ATP CARRIER PROTEIN 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45635:SF31">
        <property type="entry name" value="ADP_ATP TRANSLOCASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00927">
        <property type="entry name" value="ADPTRNSLCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="25"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="132"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="234"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9701982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="323" mass="35965" checksum="B28E34EA5E15B6FA" modified="2024-01-24" version="1">MGEEEGDEGVLRSRTRASGPPKWLTSFQKDLMAGAVMGGLVHTIVAPIERAKLLLQTQESNFAIVAGRHRRFKGMCDCIVRTVREEGILSLWRGNGSSVLRYYPSVALNFSLKDLYKNMLRSSNSLDGHVTYGVSANFIAGAAAGCTTLIIIYPLDIAHTRLAADVGKTEARQFRGIYHFLNTILKKDGIQGIYRGLPASLQGMVVHRGLYFGGFDTIKEMLSEESKPELALWKRWAAAQAVTTSAGLLSYPLDTVRRRMMMQSGLERPMYRSTSECWRTIYRTEGVASFYRGAVSNMFRSTGAAAILVLYDEVKKFMNWSGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ABF6</accession>
    <name>A0AA39ABF6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">HMA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_002830</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the HIPP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ABF6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000035">
        <property type="entry name" value="Heavy metal-associated isoprenylated plant protein 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22814">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN ATOX1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22814:SF302">
        <property type="entry name" value="HEAVY METAL-ASSOCIATED ISOPRENYLATED PLANT PROTEIN 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0104">Cadmium</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="6" id="KW-0636">Prenylation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMA" evidence="8">
        <location>
            <begin position="26"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9704447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="155" mass="17359" checksum="628DC5B7114FAD6C" modified="2024-01-24" version="1">MGALDHVSHLFDCSHGHSKLKKRRQLQTVEIKVKMDCEGCERKVRRAVEGMKGVTQVDVEPKHHKLTVVGYVDPAKVVSRVAHRTGKKAELWPYVPYDVVAHPYAPGVYDKKAPPGYVRNAYEDPQISRLARASSTEVRYTTAFSDENPAACAIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ACB6</accession>
    <name>A0AA39ACB6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_005133</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ACB6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050295">
        <property type="entry name" value="Plant_2OG-oxidoreductases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47991">
        <property type="entry name" value="OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="5">
        <location>
            <begin position="174"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9703709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="346" mass="39364" checksum="3248368EDA5C18EB" modified="2024-01-24" version="1">MEPTTVEPFPSETQTRLHPSFVLDEEQRPKVAYNHFSDAIPVISLAGIDEADGRRLDICQKIVEACEERGIFQVVDHGVDSHLISEMFRGKKGGFLVSSHLRGEAVRNWREFLTYFSYPLRERDYSMWPDKPEGLTVVMKEYSEKLMGLACKLLEVMSEAMGLEKEALFKACVDIDQKVLVNFYPKCPQPDLTLGLRRHTDPGTITLLLQDQVGGLQATRDGGKTWITVQPVEGAFVVNLGDHGHFMSNGRFKRAEHQVVVNSSCDRFSIATFQNPAPEAIVYALKIEEGEKAVLEEAISFQEMYVRKMNWDINRSWLKKPVKDHQSYDFDHQSKLAPKPAENIFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ACV3</accession>
    <name>A0AA39ACV3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 4">Proteasome assembly chaperone 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_003097</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Chaperone protein which promotes assembly of the 20S proteasome as part of a heterodimer with PSMG1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Forms a heterodimer with PSMG1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the PSMG2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ACV3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10900:FF:000004">
        <property type="entry name" value="Proteasome assembly chaperone 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10900:FF:000005">
        <property type="entry name" value="Proteasome assembly chaperone 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10900">
        <property type="entry name" value="PAC-like subunit"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019151">
        <property type="entry name" value="Proteasome_assmbl_chaperone_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016562">
        <property type="entry name" value="Proteasome_assmbl_chp_2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038389">
        <property type="entry name" value="PSMG2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12970">
        <property type="entry name" value="PROTEASOME ASSEMBLY CHAPERONE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12970:SF1">
        <property type="entry name" value="PROTEASOME ASSEMBLY CHAPERONE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09754">
        <property type="entry name" value="PAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF010044">
        <property type="entry name" value="UCP010044"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159659">
        <property type="entry name" value="Cgl1923-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00019186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR010044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9704884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="282" mass="31145" checksum="C6BDA3BDDD587FA5" modified="2024-01-24" version="1">MEFIVEEGKHLNNDCSTLVLPALSIGNVGQLAVDLLISSTRAERIGYLDDPYVLPCVGNDAYGPIPRGEVALPLEAYDSSLNKLTLVQQRSPVLKGMMVEFAKNLADFAADSGKKHVIVLSSLDFGQWQRIDMSSGSQIYYISSANMDGTDDDCEKLGWKKLHEYNPAQKRWKYLGTLAAGNAMQEDGLPFEDELEDEEDYYPSLPFAALFSCFKAKGLKVTCLLCYCSEGDNIPDAFNLADVACKLLELSPQNFHGDQGGNWAIPFSWKTVYGPPPDMSLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AD80</accession>
    <name>A0AA39AD80_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">inorganic diphosphatase</fullName>
            <ecNumber evidence="3">3.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_003475</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>diphosphate + H2O = 2 phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24576"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="EC" id="3.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.1" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AD80"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004427">
        <property type="term" value="F:inorganic diphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006796">
        <property type="term" value="P:phosphate-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00412">
        <property type="entry name" value="pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.80.10:FF:000007">
        <property type="entry name" value="Inorganic pyrophosphatase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.80.10">
        <property type="entry name" value="Inorganic pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008162">
        <property type="entry name" value="Pyrophosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036649">
        <property type="entry name" value="Pyrophosphatase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10286">
        <property type="entry name" value="INORGANIC PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00719">
        <property type="entry name" value="Pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50324">
        <property type="entry name" value="Inorganic pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00387">
        <property type="entry name" value="PPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9705453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="285" mass="31824" checksum="9A8ADB755B756629" modified="2024-01-24" version="1">MAVARLAVIANPLLSLNRISQVRSLRCNNGVSLSVSKRLFTCKALHNPQLHVKEQGQPETLDYRVFFHDSSGKTVSPWHDLPLHLGDGVFNFIVEIPKESSAKMEVATDEPYTPIKQDTKKGKLRYYPYNINWNYGLLPQTWEDPSFANSEVEGAFGDNDPVDVVEIGEIRAKIGQILKVKPLGALAMIDEGELDWKIVAISLDDPRASLVNDVDDVEKHFPGTLTAIRDWFRDYKIPDGKPANKFGLGNKAANKDYALKVINETNESWAKLVKRSIPAGELSLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AD88</accession>
    <name>A0AA39AD88_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_002721</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AD88"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000525">
        <property type="entry name" value="Pentatricopeptide (PPR) repeat-containing protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000755">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF386">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12854">
        <property type="entry name" value="PPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="94"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="305"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="376"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9704293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="491" mass="54717" checksum="9208DED943806912" modified="2024-01-24" version="1">MIDGLQSQSHWIPPLTSNWHSTQKFDSINGFLRHCSQTKNVRGVRKLHAHLLRTGLLFISLSFQSKLIFTYTTCLHRNNSKTFTTLLNCIHSTDPLPFNMILSDYCRSGFSFLALQTFSFMHTNGVPLDTYSFCCSLVASCSMKNVKFGAQIHARVEKSGWLSSVFVGSALVDLYAKLSLIKDAAMVFDEIPVKNTVCANALLSGYGEAKLWVEGLDFVRKMRSLNLNCDHFTLSAMLRSCAGLSAIELGRQVHGSVIRKVSDVEADVFLQSSLIEMYGKCGSAEKAGQVFNLAGFGYKGERKRDVVLWTSMLAVYGRNGHFEKVIQLYKEMLREGIRPDGVAFVTVISACGHTGHVKLGIKYFESMVSDFGLEPGPEHYSCVVDLLCRAGELEKAWKMVNEMTPKENGSYSISMWGALLNACNEFGNVELGKLAAHKALEMDPHNVGIYVLLSNMYAKFSMWDEIGQLRELMKESGLKKDVGCSWIEITS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AE68</accession>
    <name>A0AA39AE68_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NmrA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_003020</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AE68"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05259">
        <property type="entry name" value="PCBER_SDR_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008030">
        <property type="entry name" value="NmrA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050608">
        <property type="entry name" value="NmrA-type/Isoflavone_red_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045312">
        <property type="entry name" value="PCBER-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43349">
        <property type="entry name" value="PINORESINOL REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43349:SF34">
        <property type="entry name" value="PINORESINOL-LARICIRESINOL REDUCTASE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05368">
        <property type="entry name" value="NmrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NmrA-like" evidence="3">
        <location>
            <begin position="3"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9704773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="310" mass="34487" checksum="E0F0A899114C653E" modified="2024-01-24" version="1">MGKSRVLIIGATGNLGHQLAKASLQSSHPTFALVRDSAFSHPHKRHILQTLSDAGATLLEGSIEDESSLVEAMKQVDVVICAVSSKQVLDQKPVIKAIKRAGCIKKFIPSEFGLDPEKTQTSDLDHGFYSRKAEIRHLIEAEGIPYTYISCNFFMNYLLPSLVQLGAKTPPMDKVTIFGNGNVKGVFVKQSDIAAFTISAVDDPRTLNKVVYLRPPGNVYSMNELVELWESKIGKKLDKVYVTEEELLKKIKETPFPDNMDMVFIYSAFVKGDQTYFDIEASGGVDGTQLYPHQKYTTISEYLDTLLEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AFP1</accession>
    <name>A0AA39AFP1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Prokaryotic-type class I peptide chain release factors domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_001977</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AFP1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003747">
        <property type="term" value="F:translation release factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000046">
        <property type="entry name" value="Peptidyl-tRNA hydrolase ICT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000352">
        <property type="entry name" value="Pep_chain_release_fac_I"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006718">
        <property type="entry name" value="PRK09256.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47352">
        <property type="entry name" value="CLASS I PEPTIDE CHAIN RELEASE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47352:SF1">
        <property type="entry name" value="CLASS I PEPTIDE CHAIN RELEASE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00472">
        <property type="entry name" value="RF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110916">
        <property type="entry name" value="Peptidyl-tRNA hydrolase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00745">
        <property type="entry name" value="RF_PROK_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Prokaryotic-type class I peptide chain release factors" evidence="1">
        <location>
            <begin position="105"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS00745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9706773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="230" mass="25858" checksum="566DFE18FB6114C0" modified="2024-01-24" version="1">MAVIRCTTNMIVKEIFHPSLSLRSERMFQMVLPNFSVYCGTVNRGISYSRIRCAAADSGDGGRKVSARLSQVQQLLQEAEERSLSFGNEPTPKITLDHVTVSFARSGGPGGQNVNKVNTKVDMRFNVKNAYWLSDRIRERIMQMEKNRINKDGEIVISSTKTRTQKGNIEDALGKLQAIIDAASYVPPPPSEEQKKKVAKLAAIGEQKRLQNKKVLSQKKAFRRSRDSWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AFT7</accession>
    <name>A0AA39AFT7_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_001967</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AFT7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36595">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36595:SF3">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041367609" evidence="3">
        <location>
            <begin position="22"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="31"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9706757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="166" mass="19182" checksum="39E9BA696230B026" modified="2024-01-24" version="1">MSILPFLILCVASMLFLSLHSSPSIVECENLLGSSFSTVLMVFVFNTVIFAFLVKGYMPSYDEFDHFDSSLPFLLMVDGMKEEHEEDRCNNIDTSDDDDNDDNCYPCSDGYNEDDDDDGSDEEVGWADEEQDDDDLDKRIEEFIAKVNKGWREEWLRENLHDRFVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AIC6</accession>
    <name>A0AA39AIC6_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_000032</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9707764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AIC6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33673">
        <property type="entry name" value="SUPPRESSOR SRP40-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33673:SF3">
        <property type="entry name" value="SUPPRESSOR SRP40-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="137"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="280"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="331"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="331"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="343"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9707764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="378" mass="41503" checksum="8F96EBF246F9F4B2" modified="2024-01-24" version="1">MPSITMSPHNPYFPNIFHFIFFLHFRTFSINNFKINSKAKFKTKILAIFLSQSTFQFYQLAMDLEHGSGSSKHSRTHPESTVNEADPFSPAAIKLGSQFSTSSSSSSSSSADLFQVDTNLATPSKTKSDKDAQLELKDENDTGFLGPASPSQVSDLRQEPSFHNMSPTQSPPVQSMERSGDDEPYRIPSSVFARSQSTTPLEWSVASNESLFSIHVGNSSFSREHIFMLGGDHTKSGELTKSGELIVFSSSPPARLGDNVSKSVDTGKDLEMTAIANEPIKDDVPVPPTSADQNVEKIARPEVPFNSSSVRRSDASATSSRSFAFPILTEEERNVSIKEQPEKQLSQKQPQPRATEAISSYAASSSWLSCAWCPYKCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AJD6</accession>
    <name>A0AA39AJD6_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_000635</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AJD6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="243"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="267"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="310"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="372"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="413"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="670"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="779"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="942"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1065"/>
            <end position="1202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1216"/>
            <end position="1242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="251"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="318"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="417"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="490"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="673"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="706"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="849"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="944"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="963"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="983"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1005"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1145"/>
            <end position="1160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1185"/>
            <end position="1198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1218"/>
            <end position="1230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9708711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1242" mass="136295" checksum="DBB5492450810BB7" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AJV7</accession>
    <name>A0AA39AJV7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine-containing phosphotransfer protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_002557</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Functions as a two-component phosphorelay mediators between cytokinin sensor histidine kinases and response regulators (B-type ARRs). Plays an important role in propagating cytokinin signal transduction.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="2">Histidine-containing phosphotransfer domain (HPt) contains an active histidine that mediates the phosphotransfer.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AJV7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009927">
        <property type="term" value="F:histidine phosphotransfer kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043424">
        <property type="term" value="F:protein histidine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009736">
        <property type="term" value="P:cytokinin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.160">
        <property type="entry name" value="HPT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045871">
        <property type="entry name" value="AHP1-5/YPD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036641">
        <property type="entry name" value="HPT_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28242:SF5">
        <property type="entry name" value="HISTIDINE-CONTAINING PHOSPHOTRANSFER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28242">
        <property type="entry name" value="PHOSPHORELAY INTERMEDIATE PROTEIN YPD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47226">
        <property type="entry name" value="Histidine-containing phosphotransfer domain, HPT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0932">Cytokinin signaling pathway</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0902">Two-component regulatory system</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU369004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9707579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="118" mass="13987" checksum="6AADDA9B7E349DEB" modified="2024-01-24" version="1">MNLFRQYSSHMSPKKFLKGFLDGQFTQLQLQDESNPGLWLKWSLNKKKVDFKRVDSHRVKHACIVFHNYYKEQNTDVCLSCLQQVKQEYPFVKSKLETLFRLEQQILTVGGSIPIMME</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AJY9</accession>
    <name>A0AA39AJY9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NHL repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_000826</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AJY9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001258">
        <property type="entry name" value="NHL_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13833">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13833:SF57">
        <property type="entry name" value="NHL REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01436">
        <property type="entry name" value="NHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101898">
        <property type="entry name" value="NHL repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041243396" description="NHL repeat-containing protein" evidence="4">
        <location>
            <begin position="22"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="241"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="368"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="472"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9709026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="491" mass="54268" checksum="BA6D0A67EB221754" modified="2024-01-24" version="1">MGKSVLVVVLTLVVLSGGVSSALATASPAKIVTGFVSDAVPVVMKWLWSLKATTKTAISARPMMKFESGYTVETVFDGSKLGIEPYSIEVLASGELLILDSANSNLYKISSSLSQYTRPKLVTGSAEGYSGHVDGRLREARMNHPKGLTVDDKGNIYVADTLNMAIRKISDAGVTTIAGGKLGRGGGHVDGPSEDAKFSNDFDVVYIGSSCSLLVIDRGNQAIREIQLHFDDCAYQYGSGFPLGIAVLIAAGFFGYMLALLQRRVGTIVSSENDQANPSIAHSTYQKPLKSVRPPLIPTEDEMERQEEGFFGSLGKLFVYAGACIAEIFGGMIPGLKKKPHSYQYQNQQNYQQPQKHSNAWPLQESFVIPDEDEPPSIDTRTPTPRKTYPFMSKDAEKMHQIRQSRAFVSGWDGDFQQQQKQHHHRHYSSTPHTYYEQNCEKTNEIVFGAVQEQQVRREPVDIKPVNYGDPIYDHHNIRSRTHSKGYTQGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ALB7</accession>
    <name>A0AA39ALB7_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_000375</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ALB7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000007">
        <property type="entry name" value="Two-component response regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044841">
        <property type="entry name" value="LUX/BOA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006447">
        <property type="entry name" value="Myb_dom_plants"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01557">
        <property type="entry name" value="myb_SHAQKYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31442:SF40">
        <property type="entry name" value="HOMEODOMAIN-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31442">
        <property type="entry name" value="HOMEODOMAIN-LIKE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="92"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="189"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="353"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="92"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="193"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9708284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="550" mass="61187" checksum="3C5DF93836E4A720" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AM18</accession>
    <name>A0AA39AM18_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Vinorine synthase-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_001254</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AM18"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31623:SF118">
        <property type="entry name" value="BAHD ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31623">
        <property type="entry name" value="F21J9.9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9709684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="398" mass="44816" checksum="D40742B439227D4A" modified="2024-01-24" version="1">MKPEIISRETIKPSSPTPHSQRWYKLSVFDQMSYPVYIPIILFYTSNGGDNPCNDKGVDFFEARMDCHLQGFLNHPEFEALNLLAQGQIQCNNLDLRSTLIVQITFFDCGGMAIGVSMSHKVADISSMSAFLNDWATMARQSGEEEISAQFITSFFPPHGSLDIPEYVPRKINCAVRRFVFNATKLDALKTLATSHGVENPSRVQVVTALLYKCADAASRAGSDSLRASDLLQMVNLRRLVIPPLPDKSFGNLAWCFSISATEEGEVEFHDLVAQLKEGIAGFRLTYGKSFSWYELSRLVSEFRKASASSVKRKGNMVRFTCTSWCRFPLYQVDFGWGKPVWISCTNVMWNTFILMDTRSGDGIEALVSLEEQNMNVFEHDEELLRFASLNPNAFEAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ANK9</accession>
    <name>A0AA39ANK9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">YdbS-like PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_000953</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ANK9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005182">
        <property type="entry name" value="YdbS-like_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35688">
        <property type="entry name" value="NAD(P)-LINKED OXIDOREDUCTASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35688:SF2">
        <property type="entry name" value="NAD(P)-LINKED OXIDOREDUCTASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03703">
        <property type="entry name" value="bPH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="88"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="YdbS-like PH" evidence="3">
        <location>
            <begin position="113"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="55"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9709244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="201" mass="21918" checksum="99DB0FEB7CEBB6EB" modified="2024-01-24" version="1">MASTSATTSSAPILSPATTSTTHHSLQFSTKLSFTKLPSIPSSRTNRPHSKLHVSSPTNKPTATTSKSTSKPTEETIFFDGGAHYGDLVANLLLGFTLLWLPLTLAAVSRAFFLRYRFTNLRVTVISGLTGQDRSDFSYKVIKDVQVVPRFVGEWGDIVITLKDGTKVDLRSVPKFREIAQYCLSMAGQSEVLKESGAKGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ANS3</accession>
    <name>A0AA39ANS3_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_001106</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ANS3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9709468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="122" mass="13207" checksum="C45DA44845F00C73" modified="2024-01-24" version="1">MGQEPKVLVSFTIESLEIISIPAIQTIILKSILLISFTVAGILMVLPSEQIDGNPVPTIIFKGHPSIFHAFLISVSFAFNGAFTALMMESPSKFARFCGYYSIASMASAISVLMFAIFLQLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D7J1</accession>
    <name>A0AA39D7J1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_026324</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9673000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D7J1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.430:FF:000003">
        <property type="entry name" value="Probable disease resistance protein At5g66910"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057135">
        <property type="entry name" value="At4g27190-like_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050905">
        <property type="entry name" value="Plant_NBS-LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463:SF220">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23247">
        <property type="entry name" value="LRR_RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="8">
        <location>
            <begin position="169"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="25"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9673000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="927" mass="106271" checksum="1965A2487F8FF370" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D7T9</accession>
    <name>A0AA39D7T9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">cysteine synthase</fullName>
            <ecNumber evidence="4">2.5.1.47</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Cysteine synthase-like protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_025845</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate</text>
            <dbReference type="Rhea" id="RHEA:14829"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:58340"/>
            <dbReference type="EC" id="2.5.1.47"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion outer membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cysteine synthase/cystathionine beta-synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.47" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D7T9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004124">
        <property type="term" value="F:cysteine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01561">
        <property type="entry name" value="CBS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000077">
        <property type="entry name" value="Cysteine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000049">
        <property type="entry name" value="Cysteine synthase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000096">
        <property type="entry name" value="Related to cysteine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050214">
        <property type="entry name" value="Cys_Synth/Cystath_Beta-Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001216">
        <property type="entry name" value="P-phosphate_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10314">
        <property type="entry name" value="CYSTATHIONINE BETA-SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00901">
        <property type="entry name" value="CYS_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="44"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="15">
        <location>
            <begin position="84"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00078545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9672382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="469" mass="50665" checksum="C55AFEDAD9A51320" modified="2024-01-24" version="1">MKAVRWNAAITCKETRTLERPFADCSEFRRSRVRVTTTMAPVKVTGAVFAAFSIAILSYLLCNSRSRKTHKPISKKKPRRGLIEAIGNTPLIRINSLSEATGCEILGKAEFLNPGGSVKDRVAVKIIEEALESGQLAPGGVVTEGSAGSTAISLATVAPAYGCKCHVVIPDDVAIEKSQILEALGATVERVRPVSITHKDHYVNVARRRALEANELASKHGKYIGMDADGLVQANGHIPEEEKKNSVFSSNCKGGFFADQFENLANFRAHYEGTGPEIWEQTGGDLHAFVAAAGTGGTLAGVSRSLQEKSPNIKCFLIDPPGSGLFNKITRGVMYTREEAEGRRLKNPFDTITEGIGINRLTQNFLMAELDGAFRGTDMEAVEMSRYLLKNDGLFVGSSSAMNCVGAVRVAQSIGPGHTIVTILCDSGMRHLSKFYDSQYLSQHGLTPTATGLEFLGISSFNSLMSEHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D8B2</accession>
    <name>A0AA39D8B2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WAT1-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_024481</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the drug/metabolite transporter (DMT) superfamily. Plant drug/metabolite exporter (P-DME) (TC 2.A.7.4) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D8B2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030184">
        <property type="entry name" value="WAT1-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31218">
        <property type="entry name" value="WAT1-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="17"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="190"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="222"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="254"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="287"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="311"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="18"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="192"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9675578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="361" mass="39931" checksum="0C137CD069232D59" modified="2024-01-24" version="1">MKGLVGHVMAMESHRPYVAMLFIQFVYAGMALFSKAAIAKGMNPYVFVVYRQACASLALAPFAFFLERKKDAPLSYSTLCKIFLVSLCGLTLSLNLYYVAIGFTSATFAAATTNTIPAITFIMAVFLGMESIPMKHFHGIAKVLGSVVGVSGAMVFAFVKGPALKFMDWYPEIKKGISDSSVEQNSKGEWIKGSLMMLAANTAWSLWLILQGPIIKQYPAKLRLTTLQCFFSCIQSVVLAAVVERNPSSWKLAWDLNLLSIAYCGIVVTGITYWLQVWTIEKKGPVFTSMFTPLALIITAVFSAFLWKETLYWGSVGGAVLLVVGLYSVLWGKNREDGKSEILEQRQESKEEIVLECITHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DAS1</accession>
    <name>A0AA39DAS1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Mediator of RNA polymerase II transcription subunit 30</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_021893</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9676600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DAS1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034568">
        <property type="entry name" value="MED30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36406">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36406:SF2">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="41"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="123"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="52"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9676600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="174" mass="18734" checksum="61CF30420C4F9D7F" modified="2024-01-24" version="1">MEDKAMKATQELALEGQKHLEETIESAFQILSSMNDELCNPTLWSTPPPPATAASSHSSASLNGDASSDFSHHMEIAGGGALDDARLRYKSSVASLRAVLNAIQSSQKAKAFEASSTASGSASQADQAEIEKLEEHASNLRKELADKNKYLKLLIDQLRDLITDISTWQSPCSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DC78</accession>
    <name>A0AA39DC78_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">chitinase</fullName>
            <ecNumber evidence="2">3.2.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_021552</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1-&gt;4)-beta-linkages in chitin and chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.14"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the glycosyl hydrolase 18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.14" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DC78"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008843">
        <property type="term" value="F:endochitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02877">
        <property type="entry name" value="GH18_hevamine_XipI_class_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000015">
        <property type="entry name" value="Acidic endochitinase SE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045321">
        <property type="entry name" value="Cts1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001579">
        <property type="entry name" value="Glyco_hydro_18_chit_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050542">
        <property type="entry name" value="Glycosyl_Hydrlase18_Chitinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45708:SF21">
        <property type="entry name" value="ACIDIC ENDOCHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45708">
        <property type="entry name" value="ENDOCHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01095">
        <property type="entry name" value="GH18_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0146">Chitin degradation</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041436822" description="chitinase" evidence="11">
        <location>
            <begin position="26"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="GH18" evidence="12">
        <location>
            <begin position="26"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9679658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="297" mass="32054" checksum="F7BD666AC2597745" modified="2024-01-24" version="1">MARTPQSTPLLISLSVLAFLQTSYAGGIAIYWGQNGNEGTLTQTCNTGKYSYVNIAFLNKFGNGRTPEINLAGHCNPASNGCTSVSTGIRNCQNRGIKVMLSIGGGVGSYSLSSSNDAQKVANYLWNNFLGGQSPSRPLGDAVLDGIDFDIELGSTNYWDDLARALSGFSKRGRKVYLTAAPQCPFPDRFLGAALNTGLFDYVWVQFYNNPPCQYTTGNTNKLLNSWNRWTSSINSRIFLGLPASSAAAGSGFMPANVLTSQILPVIKRSPKYGGVMLWSKYYDDQSGYSSTIKSSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DCY0</accession>
    <name>A0AA39DCY0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">phosphogluconate dehydrogenase (NADP(+)-dependent, decarboxylating)</fullName>
            <ecNumber evidence="3">1.1.1.44</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_019642</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 6-phosphogluconate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.44" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DCY0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004616">
        <property type="term" value="F:phosphogluconate dehydrogenase (decarboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019521">
        <property type="term" value="P:D-gluconate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006114">
        <property type="entry name" value="6PGDH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006183">
        <property type="entry name" value="Pgluconate_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11811">
        <property type="entry name" value="6-PHOSPHOGLUCONATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00393">
        <property type="entry name" value="6PGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01350">
        <property type="entry name" value="6PGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0311">Gluconate utilization</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0570">Pentose shunt</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="6-phosphogluconate dehydrogenase C-terminal" evidence="7">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9680378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="118" mass="13136" checksum="63E44FCDD84D0573" modified="2024-01-24" version="1">MVHNGIEYGDMQLIAKAYDVPKSVGKLSNEELRDVFSEWNKGELLSFLIEISADIFGIKDDKGEGCVYEGLIQAFRGLTFVYVLLGLNGHQAYGDQTLDIVTLNLPQCWSSMVVQFGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DD73</accession>
    <name>A0AA39DD73_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Neprosin PEP catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_021383</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DD73"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1320.10">
        <property type="entry name" value="Outer-capsid protein sigma 3, large lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053168">
        <property type="entry name" value="Glutamic_endopeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004314">
        <property type="entry name" value="Neprosin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31589:SF223">
        <property type="entry name" value="PROTEIN, PUTATIVE (DUF239)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31589">
        <property type="entry name" value="PROTEIN, PUTATIVE (DUF239)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03080">
        <property type="entry name" value="Neprosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52045">
        <property type="entry name" value="NEPROSIN_PEP_CD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Neprosin PEP catalytic" evidence="1">
        <location>
            <begin position="1"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS52045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9679434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="212" mass="23428" checksum="7AE4A268B39BF233" modified="2024-01-24" version="1">MVSVSGDTDFIQVGWMVNKGVYGDGITRFFTMWTADYFETTGCMNTYCPGFVQVNSKIPLSSNFDQVSTVNGTQYDYPISIVQDQSTLDWWLIAGPNITAIGYWPRDLFPFLNTIAIHVEWGGYLQKADPTSTTAPQMGSGLFPEQGYGKAAYFKQIQIVQGEGGFVDPTADSVNLISDRPQCYKVGPSAELLFYTGYHFYYGGPGGDKCNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DDA3</accession>
    <name>A0AA39DDA3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_021444</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DDA3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080084">
        <property type="term" value="F:5S rDNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051061">
        <property type="entry name" value="Zinc_finger_trans_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46179:SF13">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46179">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="65"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="94"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="132"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="238"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="242"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="265"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9679524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="275" mass="31753" checksum="9CAF9E45839B6921" modified="2024-01-24" version="1">MGGERKEPVFGEVRRYFCDYCGICRSKKSHVNAHMLSHHQYQVGNLLRSSIKWPQSHTTCFERPFTCPVDDCHSSYRRKVHLTRHLLKHQGKLFTCPVQNCNSRFAFQSSIKRHVKEFHDESPPNNVGSKQYVCSEIGCGKVFKFASKLQTHEDSHVKLDLEVVCSEPGCFKHFTNEQCLKTCGTKQLRKNIKRHLRTHKSGCSSEGIRCHFKGCLHTFSTKTRCHVYTHGDLEELDEQFRQRPRGGRKRKSPPVEALLRKRVTPPSQSDSILSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DDM1</accession>
    <name>A0AA39DDM1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_019474</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DDM1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053258">
        <property type="entry name" value="Ca-permeable_cation_channel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34115">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34115:SF5">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9680186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="193" mass="21713" checksum="D71FA51C2F4C3B42" modified="2024-01-24" version="1">MFIDHATVINILGIDSLQQHNHNHISSTSRNSILPLLSLILPLLLDLINLKFQKEVDSLFYAHPITIKVAIAGLLVFALAFGIDFTFHSSHLSPTCAALLRTTMVFSGYLSLASLASLLFPDACRPLIYLMYVLLSLSNYLYRLIRKWCEGMMHKLQTLSTGMTRTPCLPIANMDYYYTSQIEAHVDPQVPQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DFN2</accession>
    <name>A0AA39DFN2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Phosphoribosyl-AMP cyclohydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_018939</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1-(5-phospho-beta-D-ribosyl)-5'-AMP + H2O = 1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:20049"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58435"/>
            <dbReference type="ChEBI" id="CHEBI:59457"/>
            <dbReference type="EC" id="3.5.4.19"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>1-(5-phospho-beta-D-ribosyl)-ATP + H2O = 1-(5-phospho-beta-D-ribosyl)-5'-AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22828"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:59457"/>
            <dbReference type="ChEBI" id="CHEBI:73183"/>
            <dbReference type="EC" id="3.6.1.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/9.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 3/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">In the C-terminal section; belongs to the PRA-PH family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">In the N-terminal section; belongs to the PRA-CH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DFN2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004635">
        <property type="term" value="F:phosphoribosyl-AMP cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004636">
        <property type="term" value="F:phosphoribosyl-ATP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11534">
        <property type="entry name" value="NTP-PPase_HisIE_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.810:FF:000001">
        <property type="entry name" value="Histidine biosynthesis bifunctional protein HisIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1080">
        <property type="entry name" value="MazG-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.810">
        <property type="entry name" value="Phosphoribosyl-AMP cyclohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01019">
        <property type="entry name" value="HisIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023019">
        <property type="entry name" value="His_synth_HisIE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008179">
        <property type="entry name" value="HisE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021130">
        <property type="entry name" value="PRib-ATP_PPHydrolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002496">
        <property type="entry name" value="PRib_AMP_CycHydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038019">
        <property type="entry name" value="PRib_AMP_CycHydrolase_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03188">
        <property type="entry name" value="histidine_hisI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42945">
        <property type="entry name" value="HISTIDINE BIOSYNTHESIS BIFUNCTIONAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42945:SF1">
        <property type="entry name" value="HISTIDINE BIOSYNTHESIS BIFUNCTIONAL PROTEIN HIS7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01502">
        <property type="entry name" value="PRA-CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01503">
        <property type="entry name" value="PRA-PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101386">
        <property type="entry name" value="all-alpha NTP pyrophosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141734">
        <property type="entry name" value="HisI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphoribosyl-AMP cyclohydrolase" evidence="13">
        <location>
            <begin position="86"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00008299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9683153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="289" mass="32557" checksum="1DB7E21E7149F989" modified="2024-01-24" version="1">MAVSYSHCLQSLRVTPRSRLFVSNVDCWRDNRIMKSYSPVSASSKKPQQDLSLEAKVETLLDSVKWDDKGLAVAIAQNVDTGAVLMQGFVNRDALATTISSQKATFYSRSRSKLWTKGETSLNFINIHDIFLDCDRDSIIYLGKPDGPTCHTGSETCYYSSVFDLLKNPQGEESNNLALTTLYSLESTISQRKAEVASPQNGKPSWTKRLLLDDKLLCSKIREEADELCRTHEENEDKSRTASEMADVLYHTMVLLSLKDVKMEEVLQVLRHRFSQSGIEEKKSRATQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DG15</accession>
    <name>A0AA39DG15_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_018560</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the plant CAR protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DG15"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009738">
        <property type="term" value="P:abscisic acid-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04038">
        <property type="entry name" value="C2_ArfGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044562">
        <property type="entry name" value="CAR1-11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45933:SF11">
        <property type="entry name" value="PROTEIN C2-DOMAIN ABA-RELATED 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45933">
        <property type="entry name" value="PROTEIN C2-DOMAIN ABA-RELATED 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0938">Abscisic acid signaling pathway</keyword>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="8" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="12">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9682661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="165" mass="18876" checksum="A7B9489E68A9C380" modified="2024-01-24" version="1">MEHLHGLLRIHVHRGVNLAKRDVRSSDPYVIVRMGKQKLKTRVVNKNVNPEWNEDLTLSVDDTNLPVKIFVYDRDTFSLDDKMGDAEFQICPFVEALKMKLDGLPSGTIITRVQPNRENCLAEESCIIWTEGKVVQNLVLRLRNVESGEVELQLQWIDIPGSRGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DHA9</accession>
    <name>A0AA39DHA9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Pectate lyase</fullName>
            <ecNumber evidence="4 9">4.2.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_016544</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>Eliminative cleavage of (1-&gt;4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.</text>
            <dbReference type="EC" id="4.2.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="9">Binds 1 Ca(2+) ion. Required for its activity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 2/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the polysaccharide lyase 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.2.2" evidence="4 9"/>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DHA9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030570">
        <property type="term" value="F:pectate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018082">
        <property type="entry name" value="AmbAllergen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002022">
        <property type="entry name" value="Pec_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045032">
        <property type="entry name" value="PEL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31683:SF144">
        <property type="entry name" value="PECTATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31683">
        <property type="entry name" value="PECTATE LYASE 18-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00544">
        <property type="entry name" value="Pectate_lyase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00807">
        <property type="entry name" value="AMBALLERGEN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00656">
        <property type="entry name" value="Amb_all"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0106">Calcium</keyword>
    <keyword evidence="8 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Pectate lyase" evidence="10">
        <location>
            <begin position="31"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9684101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="331" mass="37169" checksum="7999C1A64F0AFADE" modified="2024-01-24" version="1">MASLPYTDVDSNLRDLAGKAEGFGRLAIGGLHGPIYSVTTLADDGPGSLREGCRRREPLWIVFEISGTINLSSYLSVSSYKTIDGRGQRIKFTGKGLRLKECEHIIICNLEFEGGRGHDVDGIQIKPNSRHIWIDRCGLHDYDDGLIDITRQSTDITVSRCYFSQHDKAMLIGADPSHIGDRCIRVTIHHCFFDGTRQRQPRVRFGKVHLYNNYTRNWSVYAVCASVESQIYSQNNIYEAGEKKVTFKYYTEKAADMEEAKSGLIRSEGDLFLNGAQSCLLTGIGEECIFHPSEYYPTWTMEMASDSLKNVLQVCTGWQAIPRPAELMTSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DI33</accession>
    <name>A0AA39DI33_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_017159</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DI33"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14703">
        <property type="entry name" value="bZIP_plant_RF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044797">
        <property type="entry name" value="At4g06598-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044759">
        <property type="entry name" value="bZIP_RF2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46835:SF4">
        <property type="entry name" value="B-ZIP PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46835">
        <property type="entry name" value="BASIC-LEUCINE ZIPPER (BZIP) TRANSCRIPTION FACTOR FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="228"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9685016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="308" mass="34229" checksum="0CDAFFCD763D1819" modified="2024-01-24" version="1">MSRQANLPPRCPFQKKPITRPIHDPIFPNLGNDNELYPRHHKSSSQSSISEEQPAWFDDLLNGPDSNLRGIFHRRSASDSVALLDGVVDHFPSLQPLKDDENSVCDRIGSGLESACMYGPNSPRQRGNQSFSENAIASALSEYVSKSPLEDLDGSFCISGINQFDLKENACGADGELNAETKAVKRHSGQRSRVRKLQYIAELERTVDVYQTLESELAIRVASLLQLRVALSMENSKLKQQLAKLQQQKLIMDGQHKSLRKEVERLKGGLADSINSQIRTYTGSNTGAEAVRSEVSWLKLDVGKLNLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DIJ0</accession>
    <name>A0AA39DIJ0_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_017499</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DIJ0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35482">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35482:SF1">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9685481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="108" mass="12430" checksum="7FA7735F887AFA80" modified="2024-01-24" version="1">MVDRPDEARVMYERLQTHPNALVSKKARQFIFGFQAMEMMKVTSQLPPKNTGYQNFFEAFIEDKADYSLKDNEVEVEVEVELGQALPYIIFLVSPIFIVLLIALQKGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DJG8</accession>
    <name>A0AA39DJG8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein argonaute 16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_015442</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the argonaute family. Ago subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DJG8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02846">
        <property type="entry name" value="PAZ_argonaute_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04657">
        <property type="entry name" value="Piwi_ago-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000091">
        <property type="entry name" value="Protein argonaute 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.260.10:FF:000008">
        <property type="entry name" value="Protein argonaute 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.260.10">
        <property type="entry name" value="paz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014811">
        <property type="entry name" value="ArgoL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032472">
        <property type="entry name" value="ArgoL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032473">
        <property type="entry name" value="Argonaute_Mid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032474">
        <property type="entry name" value="Argonaute_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003100">
        <property type="entry name" value="PAZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036085">
        <property type="entry name" value="PAZ_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003165">
        <property type="entry name" value="Piwi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045246">
        <property type="entry name" value="Piwi_ago-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22891">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08699">
        <property type="entry name" value="ArgoL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16488">
        <property type="entry name" value="ArgoL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16487">
        <property type="entry name" value="ArgoMid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16486">
        <property type="entry name" value="ArgoN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02170">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02171">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01163">
        <property type="entry name" value="DUF1785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00949">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00950">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101690">
        <property type="entry name" value="PAZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50821">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50822">
        <property type="entry name" value="PIWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="3" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="PAZ" evidence="8">
        <location>
            <begin position="271"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="Piwi" evidence="9">
        <location>
            <begin position="557"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="13"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="142"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9686561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="904" mass="100939" checksum="88F17C2C0976C09D" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DJW7</accession>
    <name>A0AA39DJW7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">HTH myb-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_015243</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DJW7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000007">
        <property type="entry name" value="Two-component response regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044825">
        <property type="entry name" value="GLK1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006447">
        <property type="entry name" value="Myb_dom_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01557">
        <property type="entry name" value="myb_SHAQKYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31312">
        <property type="entry name" value="TRANSCRIPTION ACTIVATOR GLK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31312:SF1">
        <property type="entry name" value="TRANSCRIPTION ACTIVATOR GLK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="7">
        <location>
            <begin position="152"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="70"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="283"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="83"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="116"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="290"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9686245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="432" mass="47211" checksum="BB3000CB6CF6AA7A" modified="2024-01-24" version="1">MLAVSPLRSTNDEREGEMESFSIGAGDFPDFSEGNLLECIDFDDLFIGLHDGEILPDLEMDPEILAEFSVSAGEESDVNTSVTEKKVEDNPKKEEEDKVSGTGSGSGLGSGSSSSRGEEIVSKRSESVATNASPKEVEKGRKSSAQSKNPQGKRKVKVDWTPELHRRFVQAVEQLGVDKAVPSRILEIMGIDCLTRHNIASHLQKYRSHRKHLLAREAEAASWSQKRQIYGVAAGGGGHKREMGGWIPPTMGFPHITPMHHHPFRPLHVWGHPTVDQSAMHMWPKHLPHSPSPPPPPPPTWPPSATPPSPSPDPYWHPHHQRVPNGLTQGTPCFPQPLAAPRFATPPVTGIPPHPMYKLDHHHPGIGVPARHSGPFPLFDFHPSKETVDAAIGDALSKPWQPLPLGLKPPSLDGVMVELQRQGVPKIPPTCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DK94</accession>
    <name>A0AA39DK94_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_016051</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DK94"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48227:SF1">
        <property type="entry name" value="DNA LIGASE 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48227">
        <property type="entry name" value="DNA TOPOISOMERASE 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9687401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="130" mass="14593" checksum="91937D9469A6830D" modified="2024-01-24" version="1">MKTISGRVTSSNPISLSKATSVLTTFVAAETGASQAVAAYLRRTVEAFNELTQLHRELKGSKSGRKHKRAKEGHENPRLSELTNEYDIETESAKGIDEKKKKKRKSVEVEEGGVEYSEEQQRKKKRKKSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DKG2</accession>
    <name>A0AA39DKG2_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_015926</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DKG2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9687249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="195" mass="21055" checksum="DD708498E93B4ED5" modified="2024-01-24" version="1">MNHQTPLSARNLLAVIQEPQSYVLPIIPKRLPKVVVLGEYYVLKDLPFYEEACEADAKAQGTLRRALSENGRISSSAACPPVKKKKSSVKAVKTSGPALASPFASTPSASTSTDSSASTLEVNLDLLNFGQSDSGILHSEPEPIALGAINEPKLEEGMPTDLRPDFKERHHKCLHEAITVDTPPTKRTCPEGVQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DKI7</accession>
    <name>A0AA39DKI7_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_016274</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DKI7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17414">
        <property type="entry name" value="MFS_NPF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000109">
        <property type="entry name" value="POT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11654">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00854">
        <property type="entry name" value="PTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="51"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="114"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="163"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="205"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="239"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="344"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="393"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="426"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="467"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="499"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="548"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="31"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9687731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="609" mass="66969" checksum="B5A35B01EA0A6B60" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DNH8</accession>
    <name>A0AA39DNH8_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_014241</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DNH8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041437980" evidence="2">
        <location>
            <begin position="25"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9688477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="452" mass="34750" checksum="67B6E9D2A57FC326" modified="2024-01-24" version="1">MAGKFGCGVLWVWVVVVGCACVEGRKLDEKNDGGLKKPEWFFDGNPGWGGGGLGGGSGGWKGQSFGHSFSFGKGVGFSFGSGKPGIGGKPDCVGGGGGGGGGGGSGGGGGIGGGGGIGGGSGGGIGGGGGKHHKKKEKKQHYGGGVGGGIGSGGGTGGGVGGGGGTGGGVGGGGGTGGGVGGGGGGGTGGGIGGGGGAGGGGGIGGGAGGGIGKGIGGGGGAGGGGAGGGIGKGIGGGGGAGGGGGGGGIGGGAGGGIGKGIGGGGGAGGGGGGGGIGGGAGGGIGKGIGGGGGAGGGGAGGGIGKGIGGGGGAGGGVGGGGAGGGIGKGIGGGGGMGGGGGGIGKGIGGGAGGGFGGGIGKGIGGGIGGGGGGGGGIGKGIGGGAGGGFGGGVGKGIGGGGGGFGGGGGGGSGGGIGGGFGGGSGVGGGIGDGGGGFGGGGGAGGGIGGGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DPV9</accession>
    <name>A0AA39DPV9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_011084</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9691794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DPV9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000062">
        <property type="entry name" value="protein STRUBBELIG-RECEPTOR FAMILY 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000095">
        <property type="entry name" value="protein STRUBBELIG-RECEPTOR FAMILY 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000125">
        <property type="entry name" value="Protein STRUBBELIG-RECEPTOR FAMILY 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046959">
        <property type="entry name" value="PRK1-6/SRF4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48007">
        <property type="entry name" value="LEUCINE-RICH REPEAT RECEPTOR-LIKE PROTEIN KINASE PXC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48007:SF13">
        <property type="entry name" value="PROTEIN STRUBBELIG-RECEPTOR FAMILY 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041347081" description="Protein kinase domain-containing protein" evidence="12">
        <location>
            <begin position="24"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="288"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="408"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="235"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="313"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="701"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="245"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="267"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="315"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="715"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="437"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9691794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="724" mass="78559" checksum="E3BB7720F09A8C59" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DT32</accession>
    <name>A0AA39DT32_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein BIC1-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_010553</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DT32"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009785">
        <property type="term" value="P:blue light signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22645">
        <property type="entry name" value="BIC1_CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040374">
        <property type="entry name" value="BIC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34207">
        <property type="entry name" value="PROTEIN BIC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34207:SF2">
        <property type="entry name" value="PROTEIN BIC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9695109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="180" mass="20177" checksum="693F07185A3F767F" modified="2024-01-24" version="1">MAEEETNSMDRQTSSEHPNHPIDVSVAPERKKPKHHQPCEDQVVEPHNPFDADQAESQRQSPVTRDECSTRDETELQAGSSCAVGDVSKEPIKTETLASSEDNGRERLKRHRVEVAGSVWIPDIWGQEELMKDWIDCSVFDASLVTSRIMSARAALVAEGRRPSPSGLRIENRCKIYSLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DWE9</accession>
    <name>A0AA39DWE9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA sliding clamp PCNA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_007585</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processivity during elongation of the leading strand.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the PCNA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DWE9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043626">
        <property type="term" value="C:PCNA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030337">
        <property type="term" value="F:DNA polymerase processivity factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006275">
        <property type="term" value="P:regulation of DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019985">
        <property type="term" value="P:translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00577">
        <property type="entry name" value="PCNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.70.10.10:FF:000001">
        <property type="entry name" value="Proliferating cell nuclear antigen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.70.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00317">
        <property type="entry name" value="DNApol_clamp_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046938">
        <property type="entry name" value="DNA_clamp_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000730">
        <property type="entry name" value="Pr_cel_nuc_antig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022649">
        <property type="entry name" value="Pr_cel_nuc_antig_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022659">
        <property type="entry name" value="Pr_cel_nuc_antig_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022648">
        <property type="entry name" value="Pr_cel_nuc_antig_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00590">
        <property type="entry name" value="pcna"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11352">
        <property type="entry name" value="PROLIFERATING CELL NUCLEAR ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11352:SF0">
        <property type="entry name" value="PROLIFERATING CELL NUCLEAR ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02747">
        <property type="entry name" value="PCNA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00705">
        <property type="entry name" value="PCNA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00339">
        <property type="entry name" value="PCNACYCLIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55979">
        <property type="entry name" value="DNA clamp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01251">
        <property type="entry name" value="PCNA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00293">
        <property type="entry name" value="PCNA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proliferating cell nuclear antigen PCNA N-terminal" evidence="8">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Proliferating cell nuclear antigen PCNA C-terminal" evidence="9">
        <location>
            <begin position="127"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9698589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="266" mass="29576" checksum="2F63555859182417" modified="2024-01-24" version="1">MLELRLVQGSLLKKVLEAIKDLVNDANFDCSATGFSLQAMDSSHVALVALLLRSEGFEHYRCDRNISMGMNLNNMSKMLKCAGNDDIITIKADDGSDTVTFMFESPTQDKIADFEMKLMDIDSEHLGIPEAEYHAIVRMPSSEFQRICKDLSSIGDTVVISVTKEGVKFSTRGDIGTANIVCRQNTTVDKPEEATVIEMNEPVSLTFALRYMNSFTKATPLSNTVTISLSSELPVVVEYKIAEMGYIRFYLAPKIEEDEDETKPQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DXR0</accession>
    <name>A0AA39DXR0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dirigent protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_007100</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Secreted</location>
            <location evidence="4">Extracellular space</location>
            <location evidence="4">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the plant dirigent protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9697807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DXR0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009699">
        <property type="term" value="P:phenylpropanoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.480.10">
        <property type="entry name" value="Allene oxide cyclase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044859">
        <property type="entry name" value="Allene_oxi_cyc_Dirigent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004265">
        <property type="entry name" value="Dirigent"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21495">
        <property type="entry name" value="NUCLEOPORIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03018">
        <property type="entry name" value="Dirigent"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0052">Apoplast</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9697807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="192" mass="21155" checksum="FE13C59CF26FCF0B" modified="2024-01-24" version="1">MAKALLISPSYFIFSVFLVFSYTTIPIFGEEYSYAKTMDLEKMNLPKEKVTHFQLYWQDVVSGSNATSVTVIDALNNSTFFGSVNIIDNSLTVGPNLSSKTVGKSQGLYASTGLEESSLLMVMNFAFTDGKYNGSTFTVVGRNNVNAKVREMPIIGGSGLFRFARGYVLASTYSFSDNGDGTIEYNCYVIHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DYJ9</accession>
    <name>A0AA39DYJ9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">UDP-glycosyltransferases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_007623</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DYJ9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035251">
        <property type="term" value="F:UDP-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000056">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050481">
        <property type="entry name" value="UDP-glycosyltransf_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48048">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48048:SF45">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9698647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="164" mass="18604" checksum="39CF62F34FF42BF7" modified="2024-01-24" version="1">MLPEGFMERMGGKGMICGWAPHVKVLAHEAIAGFVSHCGWNSILESVWNSVPIVIWPLYAEQKLNTFEMVKELGLAVEMRLDSRFDGDVVMAEEIDGAIRRVMEADSTVRKMVKEMGEKSRRALMEGGSSYNSFERLIHAMIKYPVTSTSLFSFYYQFQMFGSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E0C3</accession>
    <name>A0AA39E0C3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MPBQ/MBSQ family SAM-binding methyltransferase profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_006015</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. MPBQ/MBSQ MT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E0C3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051741">
        <property type="term" value="F:2-methyl-6-phytyl-1,4-benzoquinone methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000095">
        <property type="entry name" value="2-methyl-6-phytyl-1,4-hydroquinone methyltransferase, chloroplastic-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044649">
        <property type="entry name" value="MPBQ/MSBQ_MT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031164">
        <property type="entry name" value="SAM_MPBQ_MSBQ_MT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44516">
        <property type="entry name" value="2-METHYL-6-PHYTYL-1,4-HYDROQUINONE METHYLTRANSFERASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44516:SF4">
        <property type="entry name" value="2-METHYL-6-PHYTYL-1,4-HYDROQUINONE METHYLTRANSFERASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51734">
        <property type="entry name" value="SAM_MPBQ_MSBQ_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="306"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="MPBQ/MBSQ family SAM-binding methyltransferase profile" evidence="3">
        <location>
            <begin position="68"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="SAM motif I" evidence="1">
        <location>
            <begin position="117"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="SAM motif II" evidence="1">
        <location>
            <begin position="162"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="SAM motif III" evidence="1">
        <location>
            <begin position="203"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9700492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="340" mass="38635" checksum="142A97D00A9FA0D9" modified="2024-01-24" version="1">MASVMLSGAESLKPIRAIAPSGIGLAGSDFPKRFSSQKALVCYTRNPRTRTIAPKCSLSSSRPVSQPRFIQHKQEAFWFYRFLSIVYDHIINPGHWTEDMRDEALEPADLYDRNMTVVDVGGGTGFTTLGIVKHVDAKNVTILDQSPHQLAKAKEKEPLKECRIIEGDAEDLPFPTDYADRYISAGSIEYWPDPQRGIREAYRVLKIGGKACIIGPVHPTFWLSRFFADMWMLFPKEEEYIEWFRKAGFKNVQIKRIGPKWYRGVRRHGLIMGCSVTGEKPYTGDSPLQLGPKVEDVKKPVNKFVFLLRLILGAIVAAYFLVIPIYMWLKDKIVPKGWPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E0R2</accession>
    <name>A0AA39E0R2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Serine carboxypeptidase-like 18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_003387</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E0R2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019748">
        <property type="term" value="P:secondary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12670:FF:000002">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000072">
        <property type="entry name" value="Serine carboxypeptidase-like 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12670">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF335">
        <property type="entry name" value="SERINE CARBOXYPEPTIDASE-LIKE 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041362148" description="Serine carboxypeptidase-like 18" evidence="2">
        <location>
            <begin position="22"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9705326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="462" mass="52083" checksum="2A2983A50B3278A2" modified="2024-01-24" version="1">MNLQYFLLLPLLLFSATAVVSQNIVRTLPGFSGELPFKLETGYVSVGDIEFFYYFVESQSNPGADPLILYINGGPGCSGLNGFVYQVGPVAFNITDYTGGLPTLLLYPHSWTKTANIIFLDAPVGTGFSYATTTQAYTTSDTLSAIQTTGFLKNWLNDHPDFKSNPFFLGTDSYSGILAPIIAQEIIDGNEVGEEPHINLKGYLIGCPHTDTTLEKNSRIPYAHRMALISDSLYEAAKTSCNGRYVDVEPSNAKCVEAIESILLCIEQISLQNILEPNCAFLTPKQNKEIRRSLQENSRSFLLPSQHTTGDAWCRNFEYLLSDIWTNYKSVQEALYVRPGTVKEFFRCNISLSYTVNVNNVVGYHKNLTNSGLQVLVFSGDHDMVIPHVGIEQWIKSLNISIDKDWRPWYVDGQVAGYTRKYTNNGYRLTYSTIKGAGHSPPEYKRRECYEMFYRWIHYYPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E1L2</accession>
    <name>A0AA39E1L2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_006942</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E1L2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032440">
        <property type="term" value="F:2-alkenal reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08295">
        <property type="entry name" value="double_bond_reductase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000121">
        <property type="entry name" value="Prostaglandin reductase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041694">
        <property type="entry name" value="ADH_N_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045010">
        <property type="entry name" value="MDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43205">
        <property type="entry name" value="PROSTAGLANDIN REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43205:SF7">
        <property type="entry name" value="PROSTAGLANDIN REDUCTASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16884">
        <property type="entry name" value="ADH_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="2">
        <location>
            <begin position="59"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9701782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="344" mass="38089" checksum="6872C6FF81FAF115" modified="2024-01-24" version="1">MADGEEVGNTQVIFRGYVSGFPNESDLYLANSRISLKVPEGSKAVVVKNLYLSCDPYMRLRMSYDANYFEPFRPGSVMTGNGVAKVMDSGHPSFSKGDLVWGITRWEEYTLVTATESLFKIPDTDVPLSYYIGILGVPGLTAYVGFYELCTPKKGEYVYVSSASGAVGQLVGQFAKLLGCYVVGSAGTKEKVDLLKNKFGFDEAFNYKEEQDLATCLKRYFPEGIDIYFENVGGAMLDAVLPNMRVHGRIAACGMISQYNLDKPEGIYNLMNVISKQIKMQGFITPSYFHLYPKFMETILPHIKEGKVAYVEDIAEGLQNAPSALIRLFSGHNVGKQVIQVAME</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E208</accession>
    <name>A0AA39E208_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Magnesium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_003370</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Magnesium transporter that may mediate the influx of magnesium.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the CorA metal ion transporter (MIT) (TC 1.A.35.5) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E208"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12823">
        <property type="entry name" value="Mrs2_Mfm1p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.128.330:FF:000001">
        <property type="entry name" value="Magnesium transporter MRS2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.340">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039204">
        <property type="entry name" value="MRS2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13890:SF35">
        <property type="entry name" value="MAGNESIUM TRANSPORTER MRS2-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13890">
        <property type="entry name" value="RNA SPLICING PROTEIN MRS2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22099">
        <property type="entry name" value="MRS2-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0406">Ion transport</keyword>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="387"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="430"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU366041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9705278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="459" mass="50477" checksum="B1F9E0BE78045060" modified="2024-01-24" version="1">MMRNPPSIPSKSGLAPPEEDPDSVRPTGGVGAGGAAGIRKKGTGIRAWLLLDTTGQAQVVEAGKHSIMRRTGLPARDLRILDPLLSYPSTVLGRERAIVINLEHIKAIITCQEVLLLNSKDPSVTPFVEELQRRLLRLHHATKSHEGGVHASNTDWTNLYDLGEPQSRVVSPQNFSGDFSQFQDEDEGAKADGKPGLENQDGLKVLPFEFIALEACLEAACSCLENEVRDELEHLLDDDEDMAEMYLTDKMIQQHLENSSVSSIHERDGMDDGVLHADMDEGIPAEISLEANGVSATYEGDLQNIDNPQEHLFGTPHALGRDSHGTHTSTTHSAISKHLDVEELEMLLEAYFVQIDGTLNKLSTLREYVDDTEDYINIMLDDKQNHLLQMGVMLTTATLVVSAFVVVAGIFGMNIHIELFDDKPSGLQDFLWTVGGGATGSIFLYVIAIAWCRHKRLLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E327</accession>
    <name>A0AA39E327_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Carboxypeptidase</fullName>
            <ecNumber evidence="11">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_004906</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Probable carboxypeptidase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the peptidase S10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="11"/>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E327"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019748">
        <property type="term" value="P:secondary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12670:FF:000001">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000143">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12670">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033124">
        <property type="entry name" value="Ser_caboxypep_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF346">
        <property type="entry name" value="CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00560">
        <property type="entry name" value="CARBOXYPEPT_SER_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 11" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041490415" description="Carboxypeptidase" evidence="11">
        <location>
            <begin position="26"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9703297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="496" mass="55292" checksum="15D46C42F0FEE20A" modified="2024-01-24" version="1">MAMASFYLFSSSLCMLFSFVVFTEAAPKGSLITHLPGFNGIFPSKHYSGYVDIGGEPAKNLFYYFVVSERSPAKDPLVLWLNGGPGCSSFDGLVYEHGPFNFEAGKTPKSLPTLHLNPHSWSKVSSIIYLDSPTGVGFSFSKNTRQYKTGDVQTASDTHEFLLRWFKEFPEFNTNPFYVSGESYAGVYVPTLSAAIVKGIKSGAKPTINFKGYLVGNGVTDMEFDANALVPFTHGMGLISSEMFEKARDNCGGNYYSNESKSCIEELNKIYNALSGLNQYDILEPCYHRPAKKGEETGNTTLPLSFKQLGATNRSLPVRTRMFGRAWPFRAPVKDGILPLWPELMKQNPIPCTDDQVASVWLNDKGVRTAIHAQQKDVIGEWEICTGRLHYSSDSGSMLQYHRNLTAKGYRALIYSGDHDMSVPFTGSEAWTRSLGYKIVDKWRSWISNDQVAGYTQGYEHGLTFLTIKGSGHTVPEYKPREALDFFGRWLEGKAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E4P0</accession>
    <name>A0AA39E4P0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="4">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_003149</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cyclophilin-type PPIase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E4P0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016018">
        <property type="term" value="F:cyclosporin A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01926">
        <property type="entry name" value="cyclophilin_ABH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000029">
        <property type="entry name" value="peptidyl-prolyl cis-trans isomerase D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000009">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF561">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0697">Rotamase</keyword>
    <keyword evidence="7 10" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="11">
        <location>
            <begin position="7"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="10">
        <location>
            <begin position="298"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9704962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="361" mass="40288" checksum="5DE13A923713A16D" modified="2024-01-24" version="1">MGRPRCYLDISIGEELEGRVVVELYNDIVPRTAENFRALCTGEKGIGPNTGVPLHYKGVCFHRVIRGFMIQGGDISAGNGTGGESIYGLKFEDENFELKHERKGMLSMANSGANTNGSQFFITTTRTSHLDGKHVVFGKVVKGMGVVRSIEHVTTGENDCPALNVLIADCGEIPAGVDDGVSNFFKDGDTYPDWPLDLDEHPNDLSWWTNAVDSVKAFGNEHFKKQDYKMALRKYRKALRYLDVCWEKEGIDEEKSSCLRKTKSLIFTNSSACKLKLGDLKGALLDTDFAMRDEENNVKALYRQGQAYMALNDIDAAAESFKKALELEPNDGGIKRELAAAKKKIADRREQERKAYSRMFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E6N7</accession>
    <name>A0AA39E6N7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Thioesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_000322</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 4-hydroxybenzoyl-CoA thioesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E6N7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016297">
        <property type="term" value="F:fatty acyl-[ACP] hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00586">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.129.10:FF:000037">
        <property type="entry name" value="acyl-acyl carrier protein thioesterase ATL3, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050563">
        <property type="entry name" value="4-hydroxybenzoyl-CoA_TE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31793">
        <property type="entry name" value="4-HYDROXYBENZOYL-COA THIOESTERASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31793:SF27">
        <property type="entry name" value="NOVEL THIOESTERASE SUPERFAMILY DOMAIN AND SAPOSIN A-TYPE DOMAIN CONTAINING PROTEIN (0610012H03RIK)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13279">
        <property type="entry name" value="4HBT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4" id="KW-0934">Plastid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9708205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="208" mass="23332" checksum="8F992D6C2FD56746" modified="2024-01-24" version="1">MLQALLSPTHMAVPASRAHTRGLRLYRPPLLLPAPQPPSNCRSPRLRSVPAVRSASGLAFDFKSGKGMSGFLDVELKVRDYELDQYGVVNNAVYASYCQHGRHELLEKIGVNADAVARTGDALALSELTLKFLAPLRSGDRFVVKVRVSDSSAARLYFEHFIFKLPNEEPILEARATAVWLDKNYRPVRIPTEIRSKLVQFLRHEESH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39EB80</accession>
    <name>A0AA39EB80_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_000732</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39EB80"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12242">
        <property type="entry name" value="OS02G0130600 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12242:SF10">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="201"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="238"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="271"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="308"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9708877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="347" mass="40452" checksum="88BDFA62D7E12DBA" modified="2024-01-24" version="1">MNFLTGSSKHSWLPIMAADTTTLSYWLNWRFFLCAIWVLSMMVVASMLVWKYEGFNKTKARRRENQKERAGSLYEDETWRTCLKEIHPGWLLGYRMIAFITLFTLLTANIVIDGGGIFYFYTQWTFALVTIYFALGTSCSICGYCQYRNKAGIDRVDYEGSDAEQGSYIAPTFRENANTSDMSKNFGTYEQSHVRKTARMWIYAFQIIFQMCAGAVMLTDLVFWLILVPFLSGKSFKLNFLIVCMHSVNAVFLIGDMILNCMRFPLFRIAYFVLWTSVFVIFQWIIHACVSMWWPYPFLDLSSPYAPAWYFGVGLMHILCYGICALIFRMKHFLLSRSFPESYQGMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YIP2</accession>
    <name>A0AA38YIP2_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_027247</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9671173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YIP2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9671173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="118" mass="13876" checksum="61DEBD0DF4F4DCC7" modified="2024-01-24" version="1">MVMEEKVEEEARASGSESSLSTLHLKLLQVEQELYRLKSRRKEDSKANIHADVEEIACLRAKVAELEWSEAKLKARVEELTREVGDRKEMLHELDKISPNFFSYFPHYFLRKIVEKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YIP5</accession>
    <name>A0AA38YIP5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">3-phosphoinositide-dependent protein kinase-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_027263</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9671194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YIP5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015919">
        <property type="term" value="P:peroxisomal membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034571">
        <property type="entry name" value="APEM9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36361">
        <property type="entry name" value="PROTEIN APEM9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36361:SF1">
        <property type="entry name" value="PROTEIN APEM9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="281"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9671194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="353" mass="39588" checksum="974AA9C17EE7099A" modified="2024-01-24" version="1">MVAIWEEIELSESYLVCCMFEEAATIASSVLKHVCDNKSTDVGEDDQLYDIMESAGMVLVQSLKELGRTLEILNELRQLFGSVTAIPIQVLLAGACFEISEGSTFGVKQFLEEFLGKWRYVDEQYYVPAGREPNVVCVGGCDRSFTLGIGEYLEVVDVYVVTLLGSVLNDMEHAFSWVEKAELPEEKRKELLMTLHSLYPPKAKLSQGSLSNSLVDDHEAHCSSSKEVILPEGHLKGLKPQYPPINGEKDRRKTVLNLSKRMDSCFWWYRSITLKLGNTRVVISNGKITLGCLIFLAYYIFRRKRATLKRIASRQVSSMKEALTSLWQLAFSYQVNPLAALQPLPAAAPGNRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YJQ9</accession>
    <name>A0AA38YJQ9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">WD repeat-containing protein 44</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_025422</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YJQ9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000712">
        <property type="entry name" value="Transducin/WD40 repeat-like superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000329">
        <property type="entry name" value="WD repeat-containing protein 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040324">
        <property type="entry name" value="WDR44/Dgr2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14221">
        <property type="entry name" value="WD REPEAT DOMAIN 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14221:SF67">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 44-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="399"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="512"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="552"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="25"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="84"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="161"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="337"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="457"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="167"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="189"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="344"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="461"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9671703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="876" mass="96084" checksum="62B8675EDEE6DAC6" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YLA9</accession>
    <name>A0AA38YLA9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Regulatory protein RecX</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_025907</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RecX family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YLA9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006282">
        <property type="term" value="P:regulation of DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01114">
        <property type="entry name" value="RecX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053924">
        <property type="entry name" value="RecX_HTH_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003783">
        <property type="entry name" value="Regulatory_RecX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33602">
        <property type="entry name" value="REGULATORY PROTEIN RECX FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33602:SF1">
        <property type="entry name" value="REGULATORY PROTEIN RECX FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02631">
        <property type="entry name" value="RecX_HTH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RecX second three-helical" evidence="5">
        <location>
            <begin position="208"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9672479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="326" mass="37803" checksum="3DA6DDFA1A803F41" modified="2024-01-24" version="1">MSTFLGNFSCKFSFQFQYRHFSVAWMKKNNALCCMKNRAYGSVPVRYIPKKSYKVEDSKSLKSSEEEEIQDCVRIRASDGGRSYRTTFAWDEKNKIQDKRPMSNLIFDAESESEEAIDCDVRVVDFEFMQEPEDVVKEFRIYQEKDYPELDVSQVSKSKQDAEKLAIELLATRAYTAVELKKKLHGKRVACDITEAVINDFQSRGLINDALYAETFSRSRWSSSSWGPRRIKQALFKKGVSEADAEKAVKLVFKDDGSGGDQESRHGLSNLAMDHLFVQASKQWLRGHDVPRETRKSRIIRWLQYRGFTWAVVSFILKKLESQYPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YNI4</accession>
    <name>A0AA38YNI4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">glycine hydroxymethyltransferase</fullName>
            <ecNumber evidence="5">2.1.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_023345</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the interconversion of serine and glycine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O = (6S)-5,6,7,8-tetrahydrofolate + L-serine</text>
            <dbReference type="Rhea" id="RHEA:15481"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="EC" id="2.1.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SHMT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.1" evidence="5"/>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YNI4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004372">
        <property type="term" value="F:glycine hydroxymethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019264">
        <property type="term" value="P:glycine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00378">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000097">
        <property type="entry name" value="Serine hydroxymethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00051">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001085">
        <property type="entry name" value="Ser_HO-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049943">
        <property type="entry name" value="Ser_HO-MeTrfase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039429">
        <property type="entry name" value="SHMT-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000586">
        <property type="entry name" value="PRK00011.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680:SF7">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00464">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Serine hydroxymethyltransferase-like" evidence="10">
        <location>
            <begin position="127"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00059150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9673731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="584" mass="64734" checksum="7B176B0D22D00659" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YPZ8</accession>
    <name>A0AA38YPZ8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Hexosyltransferase</fullName>
            <ecNumber evidence="12">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_023771</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the glycosyltransferase 8 family. Glycogenin subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="12"/>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YPZ8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02537">
        <property type="entry name" value="GT8_Glycogenin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000018">
        <property type="entry name" value="Hexosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002495">
        <property type="entry name" value="Glyco_trans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050587">
        <property type="entry name" value="GNT1/Glycosyltrans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11183">
        <property type="entry name" value="GLYCOGENIN SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01501">
        <property type="entry name" value="Glyco_transf_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9674427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="517" mass="60912" checksum="6536AA4CB9A1FC3F" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YRF8</accession>
    <name>A0AA38YRF8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_024216</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YRF8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052650">
        <property type="entry name" value="Zinc_finger_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041367">
        <property type="entry name" value="Znf-CCCH_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36886">
        <property type="entry name" value="PROTEIN FRIGIDA-ESSENTIAL 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36886:SF8">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18044">
        <property type="entry name" value="zf-CCCH_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="191"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="282"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="329"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="191"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="282"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="329"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="52"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="91"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="135"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="222"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="300"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="354"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="135"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="158"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="171"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="223"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="309"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="354"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="400"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="421"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9675178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="921" mass="102771" checksum="D86B024CEDDFBC86" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YS97</accession>
    <name>A0AA38YS97_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_024494</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YS97"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000512">
        <property type="entry name" value="Leucine-rich repeat receptor-like serine/threonine-protein kinase BAM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001492">
        <property type="entry name" value="Phytosulfokine receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052941">
        <property type="entry name" value="StomDev_PlantInt_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48004:SF59">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING N-TERMINAL PLANT-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48004">
        <property type="entry name" value="OS01G0149700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041292627" description="Leucine-rich repeat-containing N-terminal plant-type domain-containing protein" evidence="8">
        <location>
            <begin position="36"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="616"/>
            <end position="639"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9675597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="651" mass="70279" checksum="53619E9753F7A615" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YSJ2</accession>
    <name>A0AA38YSJ2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_024717</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YSJ2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052160">
        <property type="entry name" value="Gypsy_RT_Integrase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47266">
        <property type="entry name" value="ENDONUCLEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="39"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9675885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="349" mass="40552" checksum="DEFBF6786187A10F" modified="2024-01-24" version="1">MKVIQSGFWWPSLFKDAHSMCKGCDRCQRLGKLTRRNMMPLNPILIVDVFDVWGIDFMGPFPMSFGHSYILVGVDYVSKWVEAIPCRSNDHKVVLKFLKENIFSRFGVPKAIISDGGTHFCNKPFETLLAKYGVKHKVATPYHPQTSGQVELANREIKNILMKVVNVNRKDWSIKLLDSLWAYRTAYKTILGMSPYRLVYGKACHLPVEIEYKAWWAIKKLNMDLTRAGLKRCLDLNELEEMRNDAYLNSKIAKERLKKWHDQLVNQKNFAKGQRVLLYDSKLHLFPGKLKSRWTGPFIIHDVQPNGVVELLNFNSTRTFKVNGHRLKPYMESFSRDKEEFILLDPPPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YSN5</accession>
    <name>A0AA38YSN5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_024665</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YSN5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF78">
        <property type="entry name" value="OS12G0587133 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="483"/>
            <end position="778"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9675823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1215" mass="139525" checksum="86E5601AC7E3805C" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YSN9</accession>
    <name>A0AA38YSN9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="27">Wall-associated receptor kinase 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">PVL29_024721</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="25">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Serine/threonine-protein kinase that may function as a signaling receptor of extracellular matrix component. Binding to pectin may have significance in the control of cell expansion, morphogenesis and development.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="19">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="25">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YSN9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000038">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000084">
        <property type="entry name" value="Wall-associated receptor kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000043">
        <property type="entry name" value="Wall-associated receptor kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045274">
        <property type="entry name" value="WAK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025287">
        <property type="entry name" value="WAK_GUB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27005:SF468">
        <property type="entry name" value="OS01G0310500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27005">
        <property type="entry name" value="WALL-ASSOCIATED RECEPTOR KINASE-LIKE 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13947">
        <property type="entry name" value="GUB_WAK_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 19" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="13 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7 22" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041286426" description="Wall-associated receptor kinase 2" evidence="22">
        <location>
            <begin position="23"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="354"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="24">
        <location>
            <begin position="300"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="425"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="454"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00058961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="KAJ9675889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="27">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="751" mass="83341" checksum="5E5D11716AD26473" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YTP1</accession>
    <name>A0AA38YTP1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">TIR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_025048</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9676332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YTP1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045344">
        <property type="entry name" value="C-JID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011713">
        <property type="entry name" value="Leu-rich_rpt_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47186:SF63">
        <property type="entry name" value="C-JID DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47186">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20160">
        <property type="entry name" value="C-JID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07725">
        <property type="entry name" value="LRR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="TIR" evidence="3">
        <location>
            <begin position="2"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="5">
        <location>
            <begin position="178"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="5">
        <location>
            <begin position="333"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="C-JID" evidence="4">
        <location>
            <begin position="602"/>
            <end position="636"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF20160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9676332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="680" mass="78176" checksum="533B57CC379A3E81" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YUW9</accession>
    <name>A0AA38YUW9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Strictosidine synthase conserved region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_022243</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the strictosidine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YUW9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000032">
        <property type="entry name" value="Protein STRICTOSIDINE SYNTHASE-LIKE 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018119">
        <property type="entry name" value="Strictosidine_synth_cons-reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10426:SF136">
        <property type="entry name" value="PROTEIN STRICTOSIDINE SYNTHASE-LIKE 9-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10426">
        <property type="entry name" value="STRICTOSIDINE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03088">
        <property type="entry name" value="Str_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63829">
        <property type="entry name" value="Calcium-dependent phosphotriesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041324974" description="Strictosidine synthase conserved region domain-containing protein" evidence="6">
        <location>
            <begin position="27"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="Strictosidine synthase conserved region" evidence="7">
        <location>
            <begin position="150"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9677145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="336" mass="36014" checksum="099514FD534BDC8D" modified="2024-01-24" version="1">MNPTSTVAMISIFLLIFFSVPHTVLTLSVFTQLQLPPNVTGPEALAFDLLGGGPYTGVSDGRVLKYGGPSTGFTDFAYTTPTRTKAVCDGTTDPNSGPTCGRPLGVGFNYRTGQLYIADAYSGLLVVGSNGGLATPLASTAEGVPFRFLNSLDVDQLTGNVYFTDASSVYELRDITQAVENNDASGRLLKYDPSTKQVTVLIRGLSGPAGAAVSRDGSFVLVSEFIANRTHKFWLRGPKANTSELFFTFQGRPDNIKTSTTDTFWVAVNIGKSVPTTVPTGQRLDAYGNVLQTVNFEAEYGSTMISEVQGRGERSLCVGSRDTRYVGVYTLKVLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YVN0</accession>
    <name>A0AA38YVN0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_022440</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YVN0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.150.80:FF:000006">
        <property type="entry name" value="NAC domain-containing protein 100-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.80">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003441">
        <property type="entry name" value="NAC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036093">
        <property type="entry name" value="NAC_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31744:SF104">
        <property type="entry name" value="NAC DOMAIN-CONTAINING PROTEIN 100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31744">
        <property type="entry name" value="PROTEIN CUP-SHAPED COTYLEDON 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02365">
        <property type="entry name" value="NAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101941">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51005">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NAC" evidence="6">
        <location>
            <begin position="16"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="202"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9677450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="360" mass="40647" checksum="18853FA775C824DB" modified="2024-01-24" version="1">MENIAGRSVEDDQMILPPGFRFHPTDEELITHYLSEKVVDSNFSARAIGEVNLNNSEPWQLPGKAKMGEKEWYFFCVRDRKYPTGLRTNRATEAGYWKATGKDKEIFRGKSLVGMKKTLVFYQGRAPKGEKTNWVMHEYRLEGKFSLHNLPQTAKNEWVICRVFQKSSGGKKTHISGIARLSSYGNELNPSVLPPLMDASPAYTPRTRPATSQSPHVPCFSNPTDVPRNQEELFDCFNNPLLAISSNSSDIFQRFHLPNPFYSSQAATIPGTLQYPTPFLMQDQPSLRALLENHGSNMRQSQKLEREMISVSQETGLSTDINTEISSVVSNLELGKRPFEDQHVPSSSVGPVDLDPLWSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YY18</accession>
    <name>A0AA38YY18_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_000983</name>
        <name evidence="2" type="ORF">PVL29_020876</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YY18"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9678810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9709288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="75" mass="8537" checksum="EBBBBD13B6C79951" modified="2024-01-24" version="1">MSRQNTIVRLHNAGGHLRGRVNSETQFRFLPIIHRKTLQKQRSETRASPTTNSMEDKEPLKPSAVVNELSDPVKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z0P0</accession>
    <name>A0AA38Z0P0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Nephrocystin-3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_021791</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9680027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z0P0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009658">
        <property type="term" value="P:chloroplast organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45641:SF19">
        <property type="entry name" value="NEPHROCYSTIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45641">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_6G03870)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13374">
        <property type="entry name" value="TPR_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="195"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9680027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="573" mass="64255" checksum="B450365E4494D125" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z124</accession>
    <name>A0AA38Z124_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Heme O synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_019676</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z124"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008495">
        <property type="term" value="F:protoheme IX farnesyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006784">
        <property type="term" value="P:heme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13957">
        <property type="entry name" value="PT_UbiA_Cox10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.140:FF:000006">
        <property type="entry name" value="Protoheme IX farnesyltransferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00154">
        <property type="entry name" value="CyoE_CtaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006369">
        <property type="entry name" value="Protohaem_IX_farnesylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01473">
        <property type="entry name" value="cyoE_ctaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43448">
        <property type="entry name" value="PROTOHEME IX FARNESYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43448:SF2">
        <property type="entry name" value="PROTOHEME IX FARNESYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="174"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="203"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="230"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="271"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="297"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="330"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="368"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="368"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00030253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9680418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="420" mass="45206" checksum="C72A12C3F49C27DE" modified="2024-01-24" version="1">MWRNSQSLYSKLVFSRNPNPTFLASSSISSIDAIVRPFSIASDGTRTVGSTINTTSFSARDAVDLARHYGRCYCELSKARLSMLVVATSGTGFVLGSGNVIDFGGLFWTCAGTMMVAASANSLNQVFEINNDAKMKRTMRRPLPSGRLSIPHALTWASSVGVAGTAILACKANMLAAGLAASNLLLYAFVYTPLKQIHPVNTWVGAVVGAIPPLLGWAAAAGQVSLNGMILPAALYFWQIPHFMALAYLCRDDYAAGGYRMLSFADASGRRTAVVALRNSLYLIPLGFIAYDWGVTSGWFCLESSLLTLAISAAAFAFHRDRTKQKARRMFHASLLYLPVFMSGLIVHRVTDNQQGLVQDNSEKTVELSSSSGTLEQENENVKSQNKARWAAAGSPAKSPVAYASVAPFPFLPAPIYLAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z1P0</accession>
    <name>A0AA38Z1P0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Chalcone/stilbene synthase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_019832</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thiolase-like superfamily. Chalcone/stilbene synthases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z1P0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030639">
        <property type="term" value="P:polyketide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012328">
        <property type="entry name" value="Chalcone/stilbene_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011141">
        <property type="entry name" value="Polyketide_synthase_type-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11877">
        <property type="entry name" value="HYDROXYMETHYLGLUTARYL-COA SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11877:SF46">
        <property type="entry name" value="TYPE III POLYKETIDE SYNTHASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02797">
        <property type="entry name" value="Chal_sti_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Chalcone/stilbene synthase C-terminal" evidence="3">
        <location>
            <begin position="7"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9680630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="106" mass="12134" checksum="22CF97F6BB979EB5" modified="2024-01-24" version="1">MLAAGRDTLKLRSEKLECRRRALMDYGNVSSNTIFYVMEYMREGLKRKGGEEWGPALAFGPGITFQRNSDSKPVTTDNLHAYMLHFHLLSSVDLYVMECLGLWTLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z582</accession>
    <name>A0AA38Z582_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_018430</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z582"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009251">
        <property type="term" value="P:glucan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.300:FF:000003">
        <property type="entry name" value="Beta-D-glucan exohydrolase isoenzyme ExoI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1700">
        <property type="entry name" value="Glycoside hydrolase family 3 C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.300">
        <property type="entry name" value="Glycoside hydrolase, family 3, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051915">
        <property type="entry name" value="Cellulose_Degrad_GH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002772">
        <property type="entry name" value="Glyco_hydro_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036881">
        <property type="entry name" value="Glyco_hydro_3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001764">
        <property type="entry name" value="Glyco_hydro_3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036962">
        <property type="entry name" value="Glyco_hydro_3_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30620:SF77">
        <property type="entry name" value="LYSOSOMAL BETA GLUCOSIDASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30620">
        <property type="entry name" value="PERIPLASMIC BETA-GLUCOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00933">
        <property type="entry name" value="Glyco_hydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01915">
        <property type="entry name" value="Glyco_hydro_3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00133">
        <property type="entry name" value="GLHYDRLASE3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52279">
        <property type="entry name" value="Beta-D-glucan exohydrolase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041349937" evidence="3">
        <location>
            <begin position="24"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 3 N-terminal" evidence="4">
        <location>
            <begin position="71"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 3 C-terminal" evidence="5">
        <location>
            <begin position="428"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9682505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="541" mass="58533" checksum="9DB068128E66B657" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z698</accession>
    <name>A0AA38Z698_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_018841</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z698"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14702">
        <property type="entry name" value="bZIP_plant_GBF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000020">
        <property type="entry name" value="BZIP transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044521">
        <property type="entry name" value="AtbZIP8/43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045314">
        <property type="entry name" value="bZIP_plant_GBF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46324">
        <property type="entry name" value="BASIC LEUCINE ZIPPER 43-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46324:SF3">
        <property type="entry name" value="BASIC LEUCINE ZIPPER 43-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="8">
        <location>
            <begin position="82"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="163"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="135"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="186"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9683012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="198" mass="22840" checksum="AD31F49B30B6161D" modified="2024-01-24" version="1">MQSSEVTGLHYFVPSNPTPYPAHFSMAGNNAPSIHFSGFSSSPLSNFHVVSPQVHEFNPQISCFSSNSTSDEADEQQLSLINERKQRRMISNRESARRSRMRKQKHLDELWSQVVWLRNENHQLIDKLNHVSECHDQVLQENVQLKEEASELRQMVTDLQLNSPYPNLRDLEDEPSDTPYLRDESLNPSTTSSMDMIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z870</accession>
    <name>A0AA38Z870_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PVL29_019313</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="18">Plastid</location>
            <location evidence="18">Chloroplast thylakoid membrane</location>
            <topology evidence="18">Single-pass membrane protein</topology>
            <orientation evidence="18">Stromal side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the peptidase M41 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z870"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010304">
        <property type="term" value="P:PSII associated light-harvesting complex II catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19501">
        <property type="entry name" value="RecA-like_FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000001">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000001">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.760:FF:000035">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FTSH 6 chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.720.210:FF:000002">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FTSH chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.720.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.760">
        <property type="entry name" value="Peptidase M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01458">
        <property type="entry name" value="FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005936">
        <property type="entry name" value="FtsH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011546">
        <property type="entry name" value="Pept_M41_FtsH_extracell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000642">
        <property type="entry name" value="Peptidase_M41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037219">
        <property type="entry name" value="Peptidase_M41-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01241">
        <property type="entry name" value="FtsH_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23076:SF118">
        <property type="entry name" value="ATP-DEPENDENT ZINC METALLOPROTEASE FTSH 6, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23076">
        <property type="entry name" value="METALLOPROTEASE M41 FTSH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06480">
        <property type="entry name" value="FtsH_ext"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01434">
        <property type="entry name" value="Peptidase_M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140990">
        <property type="entry name" value="FtsH protease domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="9 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0934">Plastid</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0793">Thylakoid</keyword>
    <keyword evidence="13" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="20">
        <location>
            <begin position="246"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00060455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAJ9683684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="676" mass="73026" checksum="9FCA891EFDAE6402" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z8N7</accession>
    <name>A0AA38Z8N7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">Laccase</fullName>
            <ecNumber evidence="4 13">1.10.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Benzenediol:oxygen oxidoreductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Diphenol oxidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Urishiol oxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_016437</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Lignin degradation and detoxification of lignin-derived products.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>4 hydroquinone + O2 = 4 benzosemiquinone + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:11276"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17594"/>
            <dbReference type="ChEBI" id="CHEBI:17977"/>
            <dbReference type="EC" id="1.10.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="13">Binds 4 Cu cations per monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Secreted</location>
            <location evidence="2 13">Extracellular space</location>
            <location evidence="2 13">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the multicopper oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.10.3.2" evidence="4 13"/>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9683939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z8N7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052716">
        <property type="term" value="F:hydroquinone:oxygen oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046274">
        <property type="term" value="P:lignin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13849">
        <property type="entry name" value="CuRO_1_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13875">
        <property type="entry name" value="CuRO_2_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13897">
        <property type="entry name" value="CuRO_3_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000049">
        <property type="entry name" value="Laccase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000062">
        <property type="entry name" value="Laccase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033138">
        <property type="entry name" value="Cu_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002355">
        <property type="entry name" value="Cu_oxidase_Cu_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034288">
        <property type="entry name" value="CuRO_1_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034285">
        <property type="entry name" value="CuRO_2_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034289">
        <property type="entry name" value="CuRO_3_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017761">
        <property type="entry name" value="Laccase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03389">
        <property type="entry name" value="laccase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF431">
        <property type="entry name" value="LACCASE-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00079">
        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00080">
        <property type="entry name" value="MULTICOPPER_OXIDASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0052">Apoplast</keyword>
    <keyword evidence="10 13" id="KW-0186">Copper</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 13" id="KW-0439">Lignin degradation</keyword>
    <keyword evidence="7 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 13" id="KW-0964">Secreted</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041489505" description="Laccase" evidence="13">
        <location>
            <begin position="32"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="16">
        <location>
            <begin position="40"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="14">
        <location>
            <begin position="166"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="15">
        <location>
            <begin position="426"/>
            <end position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9683939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="577" mass="62974" checksum="84085BC61F49910D" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z8N8</accession>
    <name>A0AA38Z8N8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Methyltransferase</fullName>
            <ecNumber evidence="11">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_016697</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Membrane</location>
            <topology evidence="11">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the methyltransferase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="11"/>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z8N8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000043">
        <property type="entry name" value="probable methyltransferase PMT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004159">
        <property type="entry name" value="Put_SAM_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10108:SF979">
        <property type="entry name" value="METHYLTRANSFERASE PMT11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10108">
        <property type="entry name" value="SAM-DEPENDENT METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03141">
        <property type="entry name" value="Methyltransf_29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 11" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="85"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU366043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9684349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="677" mass="76619" checksum="38374BA7FE60CD7A" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z9W7</accession>
    <name>A0AA38Z9W7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein FAR1-RELATED SEQUENCE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_017226</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Putative transcription activator involved in regulating light control of development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the FHY3/FAR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z9W7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004330">
        <property type="entry name" value="FAR1_DNA_bnd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031052">
        <property type="entry name" value="FHY3/FAR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018289">
        <property type="entry name" value="MULE_transposase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006564">
        <property type="entry name" value="Znf_PMZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31669:SF160">
        <property type="entry name" value="PROTEIN FAR1-RELATED SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31669">
        <property type="entry name" value="PROTEIN FAR1-RELATED SEQUENCE 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03101">
        <property type="entry name" value="FAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10551">
        <property type="entry name" value="MULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04434">
        <property type="entry name" value="SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00575">
        <property type="entry name" value="ZnF_PMZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="8">
        <location>
            <begin position="548"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="19"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="673"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="19"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="31"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="673"/>
            <end position="691"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9685113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="836" mass="94783" checksum="23C97355D988D5D5" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z9Z8</accession>
    <name>A0AA38Z9Z8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Protein transport protein Sec61 subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_016850</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SEC61-beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z9Z8"/>
    <dbReference type="SMR" id="A0AA38Z9Z8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005784">
        <property type="term" value="C:Sec61 translocon complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030671">
        <property type="entry name" value="Sec61-beta/Sbh"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016482">
        <property type="entry name" value="SecG/Sec61-beta/Sbh"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13509">
        <property type="entry name" value="SEC61 SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03911">
        <property type="entry name" value="Sec61_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="74"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="43"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9684584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="108" mass="10915" checksum="05C84510AFF905CE" modified="2024-01-24" version="1">MVRGSSQSQASSSVTSRPSTVGMAPRGSAAATAGMRRRRLGTGSAGGGSGGFGSGSSGSNMLRFYTDDAPGLKITPTVVLVMSLCFIGFVTALHVFGKIYRHRSGGVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZD11</accession>
    <name>A0AA38ZD11_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Secretory carrier-associated membrane protein</fullName>
            <shortName evidence="7">Secretory carrier membrane protein</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_015490</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">Probably involved in membrane trafficking.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Cytoplasmic vesicle</location>
            <location evidence="7">Secretory vesicle membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the SCAMP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZD11"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055038">
        <property type="term" value="C:recycling endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032588">
        <property type="term" value="C:trans-Golgi network membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030658">
        <property type="term" value="C:transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007273">
        <property type="entry name" value="SCAMP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10687:SF76">
        <property type="entry name" value="SECRETORY CARRIER-ASSOCIATED MEMBRANE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10687">
        <property type="entry name" value="SECRETORY CARRIER-ASSOCIATED MEMBRANE PROTEIN SCAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04144">
        <property type="entry name" value="SCAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="121"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="152"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="185"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="271"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9686642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="290" mass="32879" checksum="2C4AE07E465F79E7" modified="2024-01-24" version="1">MAGRYESNPFDEEDVNPFANPGSVPPAANSRLSPLPPEPAGYRGATVDIPLDGAKDTKAKERELQAKEAELRKREQELKRREDAVARAGIVIEEKNWPPFFPLIHHDIANEIPIHQQKLQYVAFTTYLGLVLCLTWNIVAVTTAWIKGEGPTIWFLAIIYFISGVPGAYVLWYRPLYRAMRTDSALKFGWFFLFYLLHIAFCIFASVAPPIVFKGKSLTGILPAVDLLSSHALVGVSSKLQTFMMNGIISLDHCDYENYCWHVILLDHYNLEKLLSGFMVLAFFFFPFFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZDK6</accession>
    <name>A0AA38ZDK6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Exostosin GT47 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_015794</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 47 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZDK6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004263">
        <property type="entry name" value="Exostosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040911">
        <property type="entry name" value="Exostosin_GT47"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11062:SF300">
        <property type="entry name" value="EXOSTOSIN GT47 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11062">
        <property type="entry name" value="EXOSTOSIN HEPARAN SULFATE GLYCOSYLTRANSFERASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03016">
        <property type="entry name" value="Exostosin_GT47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="Exostosin GT47" evidence="7">
        <location>
            <begin position="154"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9687078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="484" mass="55554" checksum="540179699ADB6AB6" modified="2024-01-24" version="1">MQLGSNKPLILILLSLIPLLILISTLVIVSSTPRSLNSSWFSFSFSWNPEEIKPLSTALSSSASSSFNASLPDVHIAGGPYFPPPLEKKQETWKDKRYSKLERLEASLARARSSIREAARNGSLKSTHEDPDYVPQGPIYRNANAFHRSYLEMEKLFKIYVYEEGEPPMFHDGPCKSIYSTEGRFIHEMEKGSVYRTTDPDQALLYFLPFSVVMMVQYLYVPDSHEIHAIENTVIDYIHLISHNHPFWNRSLGADHFMLSCHDWGPRASTSVPYLYNNSIRVLCNANTSEGFNPSKDVSFPEIHLRTGEMSGPLGGLSPSRRPILGFFAGRLHGHIRYLLLEQWKDKDKDLQVYDQLPNGVSYDSMLKKSRFCLCPSGYEVASPRVVEAIYAECVPVLISDNYVPPFNDVLNWKSFAVQVQVRDIANIKRILMGISQTQYLRMYRRVKQVQRHFMVNAAPQRFDVFHMTIHSIWLRRLNIRIRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZE42</accession>
    <name>A0AA38ZE42_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GOST seven transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_016006</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZE42"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053937">
        <property type="entry name" value="GOST_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009637">
        <property type="entry name" value="GPR107/GPR108-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21229:SF1">
        <property type="entry name" value="GH17801P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21229">
        <property type="entry name" value="LUNG SEVEN TRANSMEMBRANE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06814">
        <property type="entry name" value="GOST_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="210"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="239"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="271"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="305"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="344"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="389"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="422"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="domain" description="GOST seven transmembrane" evidence="8">
        <location>
            <begin position="206"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="457"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="460"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="489"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF06814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9687345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="499" mass="55935" checksum="8C9196D1629B265C" modified="2024-01-24" version="1">MASSPSSPLLLTFVSSINASIHLYDHQGFREVGNSYLLSGGSEGIVASSTPGTAISHAHSFIRFENITFWRSKEAADQHSDMGHSTGLIQVVIFEAADRDNIGGSAYGGQRSICCTPDLAKLEGCKQGEVIRIPSATDINWPIVLNVQFSGKSLSTSMANSEVPITKTGMYNLFFISCDPKLKGLVMNGKTLWKNPDGYLPGRMAPLMKFYVYMSLAYVLLCVTWFFQYMRFWKDILQLQHCITVVIALGLFEMILWYFEFENFNRTGTRPVGITTWVVTVGAVRRTVSRLLILSVSMGYGVMRPTLGGLTSKVLLLGITYFVATELLDITEYVGTINDVSGRARLFLVLPDAFLDAFLILWIFTSLSKTLEQLQAKRSSVKLDTYRKFSNSLAVAVIASVVWIGYEVYFKATDPFNERWQSAWIITAFWDILAFGLLCVICYLWAPSQSSQRYAYSDEKGEEFDDEETQSLTRGKPEGDISLAKQDNLEDEEEEDKRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZF17</accession>
    <name>A0AA38ZF17_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_016233</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZF17"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054722">
        <property type="entry name" value="PolX-like_BBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34222:SF83">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34222">
        <property type="entry name" value="GAG_PRE-INTEGRS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22936">
        <property type="entry name" value="Pol_BBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="2">
        <location>
            <begin position="174"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9687664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="343" mass="37772" checksum="DACE04258BC15198" modified="2024-01-24" version="1">MSWLLGSVEPHIVTHLRPHRSAQSMWAYLKKVYHQDNDARRFQLEHAIAMFQHGSLSIQDYYSAFLTLWHEYADLVTADVSIATLSTIQTIHATTQRDQFLMKLRSEYESVRSSLLNRSPVPSLDICFGELLREEQRLSTQAILEQSHGSSGTATVAYAAQGRGPPMHSKNLQCFCCKEYGHIAATCPKKFCSYCKKKGHIIKECRIRPQNRQAQAFQTSIIVPPVATPAAHDSPSAACSVPAPPVPDYCTPEMVQWMLISALSAMGFQGNNSTKLWYVDAGASNHMTNNPTALCHVRPYAGQSSIQTANGSSLPIAAIGDASSKFTDVFLAPQLSTNLISVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZK18</accession>
    <name>A0AA38ZK18_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_012829</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZK18"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010333">
        <property type="term" value="F:terpene synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016102">
        <property type="term" value="P:diterpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.600.10:FF:000036">
        <property type="entry name" value="cis-abienol synthase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.130:FF:000002">
        <property type="entry name" value="Ent-copalyl diphosphate synthase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.130">
        <property type="entry name" value="Terpene synthase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001906">
        <property type="entry name" value="Terpene_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036965">
        <property type="entry name" value="Terpene_synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050148">
        <property type="entry name" value="Terpene_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005630">
        <property type="entry name" value="Terpene_synthase_metal-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31739:SF25">
        <property type="entry name" value="(E,E)-GERANYLLINALOOL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31739">
        <property type="entry name" value="ENT-COPALYL DIPHOSPHATE SYNTHASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01397">
        <property type="entry name" value="Terpene_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03936">
        <property type="entry name" value="Terpene_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01014">
        <property type="entry name" value="Terpene_Cyclase_Like_1_N-term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Terpene synthase N-terminal" evidence="5">
        <location>
            <begin position="203"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="Terpene synthase metal-binding" evidence="6">
        <location>
            <begin position="479"/>
            <end position="717"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9690377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="843" mass="96532" checksum="4BB860C2EAE3D80F" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZKL2</accession>
    <name>A0AA38ZKL2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Trans-resveratrol di-O-methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_012942</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZKL2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009717">
        <property type="term" value="P:isoflavonoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000213">
        <property type="entry name" value="Coniferyl alcohol 9-O-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000057">
        <property type="entry name" value="O-methyltransferase ZRP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016461">
        <property type="entry name" value="COMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001077">
        <property type="entry name" value="COMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012967">
        <property type="entry name" value="COMT_dimerisation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11746">
        <property type="entry name" value="O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08100">
        <property type="entry name" value="Dimerisation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00891">
        <property type="entry name" value="Methyltransf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005739">
        <property type="entry name" value="O-mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51683">
        <property type="entry name" value="SAM_OMT_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="O-methyltransferase dimerisation" evidence="6">
        <location>
            <begin position="21"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="O-methyltransferase C-terminal" evidence="5">
        <location>
            <begin position="135"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR005739-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9690545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="361" mass="40752" checksum="D6B6A6A9B0F9DA92" modified="2024-01-24" version="1">MDLIHGDGGSELFQAQSHMYKHIFNYINSMALKCAVELGIPDIVHTHNHPITLPQLVSDLHLPLTKTSYVNRLMRVLVHSGFFATAKVHENQEEEDEGYILTPSSRLLLSKDNNNVPNLSAFVLAMLDPVLVTPWHFLGDWFRGNDLTAFDTAHGKSFWDYGSHDHKFFNLFNEAMASDSRMMSLVIKDCRPVFEGLRSLVDLGGGKGLIARIISEAFPQLKCTVFDLPHVVSNFPECGNLKYVGGDMFQSVPTADAILLKLVLHSLSDEECVKILKKCREAIPSKEEGGKVIVIDIVINEKKEEHDITEAKLLFDLLMMSLVTGRERNEKEWEKLFLEAGFSHYKINPIFGLRSLIEVFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZKN2</accession>
    <name>A0AA38ZKN2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RNA ligase/cyclic nucleotide phosphodiesterase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_013124</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZKN2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004113">
        <property type="term" value="F:2',3'-cyclic-nucleotide 3'-phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009187">
        <property type="term" value="P:cyclic nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1140.10:FF:000007">
        <property type="entry name" value="Cyclic phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1140.10">
        <property type="entry name" value="Cyclic phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012386">
        <property type="entry name" value="Cyclic-nucl_3Pdiesterase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009097">
        <property type="entry name" value="Cyclic_Pdiesterase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28141">
        <property type="entry name" value="2',3'-CYCLIC-NUCLEOTIDE 3'-PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28141:SF1">
        <property type="entry name" value="2',3'-CYCLIC-NUCLEOTIDE 3'-PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07823">
        <property type="entry name" value="CPDase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017903">
        <property type="entry name" value="CPDase_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55144">
        <property type="entry name" value="LigT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9690810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="217" mass="24237" checksum="704117F1B483F8D2" modified="2024-01-24" version="1">MFRDIWLIRILGFLGFVGLSLFVWSISVDPPDMTEETESRKEVYSVWGIPPEEVGARVKKVMEGLRSEFGGPAFEPHVTVVGAISLTEADALHKFRSACDGLKAYDASVDSVATGTFFYQCVYLLLHPMPEVVEASAHCTGHFGFRSSTPYMPHLSLLYGDLSEEDKKKAQEKAKVLDESISSLNFTITRLALYKTDTEDKTLTSWQKVSECDLIPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZKR7</accession>
    <name>A0AA38ZKR7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_013163</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZKR7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.330:FF:000001">
        <property type="entry name" value="Protein SRG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050295">
        <property type="entry name" value="Plant_2OG-oxidoreductases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47991">
        <property type="entry name" value="OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="7">
        <location>
            <begin position="209"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="27"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9690870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="365" mass="40945" checksum="0878B40A0CF07255" modified="2024-01-24" version="1">MEEPKEAASLGSSLLVPSVQELAKDSLTTVPPRYLRPEQDPPFSSHTSTTPLPHIPVIDFHCLLSGDGVESELEKLHHACKEWGFFQLKNHGVSSSLMEKVKAEIQEFFNLPMEEKRKFWQQPGQIEGFGQAFVVSEEQKLDWGDLFFMVTLPPHLRKPHLFPKLPLPFRDTLEVYAVELKNLAMAILGFMAKALKMEAKDMKALFEEGFQAMRMNYYPPCPQPDQVIGLTPHSDSVGLTILLQVNEMEGLQVRKNGIWIPIKALPDAFVVNIGDVLEIVTNGNYRSIEHRAVVNSVKERLSIATFYNPKIEGDVGPAPSLVTPHSPALFKNVSVADYLKGLFSRELHGKSYLDVLKIDSEAESN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZMN7</accession>
    <name>A0AA38ZMN7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">MADS-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_013303</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZMN7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1810.10:FF:000006">
        <property type="entry name" value="Agamous-like MADS-box protein AGL62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11945">
        <property type="entry name" value="MADS BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11945:SF775">
        <property type="entry name" value="MADS-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00404">
        <property type="entry name" value="MADSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="6">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9691064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="211" mass="23467" checksum="1B486571EDBBD573" modified="2024-01-24" version="1">MGRQKIEIKKIEKKSSLEVTFSKRRTGLFKKAGELCVLCGAEAAVIVFSPGRRAFVFGHPSADAVIDRFLHRETNSRALVPVGQVHGHVQRQYLEALGRLEVKREQEETVGGDGEGGFWWDAPIENMGLNELEQFRGSLEELRKKVADRVEEMNMMMMMESGPSSSTAMVEYAAPPQEYNSSGGALHGHDLATHGVPHGFDFGFGFDEHLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZQD2</accession>
    <name>A0AA38ZQD2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_012202</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9693345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZQD2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33223">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33223:SF8">
        <property type="entry name" value="OS04G0172440 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9693345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="102" mass="11745" checksum="CF7E1A450694D93E" modified="2024-01-24" version="1">MRDPKETISWDDTDDVPVATLLVGFKMPEIERYTGVGCPCIHLRLYSTVMRALGLDEVQLLALFPLSLSDTTQRWYASLGSSRRRTWEDLAQEFLRQYSFGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZQL3</accession>
    <name>A0AA38ZQL3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">NB-ARC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_011600</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZQL3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.430:FF:000003">
        <property type="entry name" value="Probable disease resistance protein At5g66910"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057135">
        <property type="entry name" value="At4g27190-like_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050905">
        <property type="entry name" value="Plant_NBS-LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463:SF220">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23247">
        <property type="entry name" value="LRR_RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NB-ARC" evidence="8">
        <location>
            <begin position="161"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="10">
        <location>
            <begin position="417"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="11">
        <location>
            <begin position="543"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein At4g27190-like leucine-rich repeats" evidence="9">
        <location>
            <begin position="752"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="36"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9692609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="899" mass="102053" checksum="CC74B37737C0DF26" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZSS1</accession>
    <name>A0AA38ZSS1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_009982</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the peroxisomal membrane protein PXMP2/4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZSS1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007248">
        <property type="entry name" value="Mpv17_PMP22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266:SF80">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04117">
        <property type="entry name" value="Mpv17_PMP22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="163"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="196"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9694265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="276" mass="30593" checksum="273B68D0AEE6DF71" modified="2024-01-24" version="1">MAPNPITLTRRSPLLFRFLGFSRNSIYSSRIPTNPIRISTVRSYSTCHFFCYPSNSSVRTVFKDLGRSEIGFSKMGSDYFRISAVSDGGSGGTGGFGDGNSGGKSEGSGGGGDWSLLSWYLALLEKYPVLTKAITSACLTLVGDLICQLVIDQVPSLDLKRTFLFTLLGLVLVGPTLHFWYLYLSKLVTIPGASGAFLRLLLDQFLFSPTFIGVFLSTLVTLEGRPSQVVPKLQQEWFSAVLANWQLWIPFQFLNFRFVPQQFQELLSKLSSTDFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZTI0</accession>
    <name>A0AA38ZTI0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_010146</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Probable component of the PAM complex at least composed of a mitochondrial HSP70 protein, TIMM44 and TIMM14. The complex interacts with the TIMM23 component of the TIM17:23 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZTI0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001405">
        <property type="term" value="C:PAM complex, Tim23 associated import motor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000001">
        <property type="entry name" value="Import inner membrane translocase subunit tim14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12763">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12763:SF51">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM14-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="7">
        <location>
            <begin position="53"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00059031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00063640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9694512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="112" mass="12014" checksum="BFCE56B702918553" modified="2024-01-24" version="1">MLFGTPLVAGLAVAAAALAGRYGIQAWQAFKARPPTLRKFYEGGFQPTMTKREAALILGIRESTPADKVKEAHRKVMVANHPDAGGSHYLASKINEAKDVMLGKTRGSESAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZUA4</accession>
    <name>A0AA38ZUA4_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_010751</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZUA4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004158">
        <property type="entry name" value="DUF247_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31170:SF25">
        <property type="entry name" value="BNAA09G04570D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31170">
        <property type="entry name" value="BNAC04G53230D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03140">
        <property type="entry name" value="DUF247"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="325"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="450"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9695436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="473" mass="53941" checksum="C1C51150B8807245" modified="2024-01-24" version="1">MEPQISICMEGAAHADTTPQPTNTNVTTGESCDEHVIDIKVWYGGDEASNIVKERTPCESGLPRIPRVPATLRDSLNPTLPQWIQPSVVSIGPYHYNNRNLQRAQKLKSVHAEKFIKESNQEKVDLYREIKTETPQLRKCYDNLGRLKYTDNQLATMFLLDGCFLLHFINCSIKADGFSNLDFTNHEIAYIKQDLFLLENQLPYKVLELLFKGAKISKPSMEEKIKDFVASHVPFPGGISEEEENQQPCHLLHYLQGVILNKPETSSPRLKQDKENQGRWRTKWDSCKNVQELRKVGIHFKPSPTSYLTDISFKSHFCTSGCLKLPIISMNTSTMIIFLNLVAFESSNTSKTTTNNLGIISYLCFLDSLIDRWDDVKELQAAGILRNFAGEQREVAQFFNVVCSKLVPNLSVYEDVILQIDNHVKRHDNSRWRKGYFEFKQKYFSSPWSFIALIGAIIGLTLTGIEAFTSVFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZV24</accession>
    <name>A0AA38ZV24_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PAR1 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_010368</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZV24"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009489">
        <property type="entry name" value="PAR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33649">
        <property type="entry name" value="PAR1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33649:SF4">
        <property type="entry name" value="PAR1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06521">
        <property type="entry name" value="PAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041318049" description="PAR1 protein" evidence="2">
        <location>
            <begin position="26"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="163"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9694858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="183" mass="19734" checksum="924051B91D334801" modified="2024-01-24" version="1">MAFSSSPSSFILFLVSSLLLHAAFGELICEELPNDKCAFSIASSGKRCLLEKYARENGEMEYQCRTSEVIVERMSEYIETDQCVSACGVDRSSVGISSDSLLEPQFTAKLCSSNCYHNCPNIIDLYFNLAAGEGVFLPDLCEARRINPHRSMVELLSSGATPGPVAPQSLSSHAAGAPALPPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZYY7</accession>
    <name>A0AA38ZYY7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Lipoxygenase</fullName>
            <ecNumber evidence="18">1.13.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">PVL29_007148</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="22">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Plant lipoxygenase may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 17">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="18">Lipid metabolism; oxylipin biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the lipoxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.-" evidence="18"/>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9697893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZYY7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016165">
        <property type="term" value="F:linoleate 13S-lipoxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034440">
        <property type="term" value="P:lipid oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031408">
        <property type="term" value="P:oxylipin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01751">
        <property type="entry name" value="PLAT_LH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.245.10:FF:000002">
        <property type="entry name" value="Lipoxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.60:FF:000005">
        <property type="entry name" value="Lipoxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.375.10:FF:000001">
        <property type="entry name" value="Lipoxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.375.10">
        <property type="entry name" value="Lipoxygenase-1, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.372.10">
        <property type="entry name" value="Lipoxygenase-1, Domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.245.10">
        <property type="entry name" value="Lipoxygenase-1, Domain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.60.20">
        <property type="entry name" value="PLAT/LH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000907">
        <property type="entry name" value="LipOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013819">
        <property type="entry name" value="LipOase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036226">
        <property type="entry name" value="LipOase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020834">
        <property type="entry name" value="LipOase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020833">
        <property type="entry name" value="LipOase_Fe_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001246">
        <property type="entry name" value="LipOase_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042057">
        <property type="entry name" value="Lipoxy_PLAT/LH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027433">
        <property type="entry name" value="Lipoxygenase_dom_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001024">
        <property type="entry name" value="PLAT/LH2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036392">
        <property type="entry name" value="PLAT/LH2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11771">
        <property type="entry name" value="LIPOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00305">
        <property type="entry name" value="Lipoxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01477">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00087">
        <property type="entry name" value="LIPOXYGENASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00468">
        <property type="entry name" value="PLTLPOXGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00308">
        <property type="entry name" value="LH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49723">
        <property type="entry name" value="Lipase/lipooxygenase domain (PLAT/LH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48484">
        <property type="entry name" value="Lipoxigenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00711">
        <property type="entry name" value="LIPOXYGENASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00081">
        <property type="entry name" value="LIPOXYGENASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51393">
        <property type="entry name" value="LIPOXYGENASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50095">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="11 17" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="15 18" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="13 17" id="KW-0408">Iron</keyword>
    <keyword evidence="4 18" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="14" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 17" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8 18" id="KW-0925">Oxylipin biosynthesis</keyword>
    <keyword evidence="6" id="KW-0934">Plastid</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="PLAT" evidence="20">
        <location>
            <begin position="89"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoxygenase" evidence="21">
        <location>
            <begin position="212"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="276"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="19">
        <location>
            <begin position="277"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU003975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAJ9697893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="903" mass="102396" checksum="86598F4F43FAFE8F" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZZC4</accession>
    <name>A0AA38ZZC4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Vacuolar fusion protein MON1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_007287</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Plays an important role in membrane trafficking through the secretory apparatus.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MON1/SAND family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZZC4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043972">
        <property type="entry name" value="FUZ/MON1/HPS1_longin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043971">
        <property type="entry name" value="FUZ/MON1/HPS1_longin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043970">
        <property type="entry name" value="FUZ/MON1/HPS1_longin_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004353">
        <property type="entry name" value="Mon1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13027">
        <property type="entry name" value="SAND PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13027:SF7">
        <property type="entry name" value="VACUOLAR FUSION PROTEIN MON1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19036">
        <property type="entry name" value="Fuz_longin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19037">
        <property type="entry name" value="Fuz_longin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19038">
        <property type="entry name" value="Fuz_longin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01546">
        <property type="entry name" value="YEAST73DUF"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FUZ/MON1/HPS1 first Longin" evidence="3">
        <location>
            <begin position="179"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="FUZ/MON1/HPS1 second Longin" evidence="4">
        <location>
            <begin position="310"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="FUZ/MON1/HPS1 third Longin" evidence="5">
        <location>
            <begin position="456"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="50"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="108"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU367048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF19037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF19038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9698118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="571" mass="63642" checksum="C40FE587C01E9034" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZZI3</accession>
    <name>A0AA38ZZI3_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_009081</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZZI3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044961">
        <property type="entry name" value="MS5/SDI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36326">
        <property type="entry name" value="PROTEIN POLLENLESS 3-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36326:SF14">
        <property type="entry name" value="PROTEIN SULFUR DEFICIENCY-INDUCED 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0802">TPR repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9697154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="214" mass="24287" checksum="96ED833E7DE6D68F" modified="2024-01-24" version="1">MQESSRRKSSRRGDKDPSCVIHKVPSGDTPYVRAKHAQAIEAIKSCRGLCPKQAQESLDNVLIDLYKVDLPRRGLQWKTYQDCSSHCKRCQVSIKQETSRKLGNLGWSYMQKSNYMAAEMVYKKAQMIDSDANKACNLALCLIKEARYTEAHSVLNEVLQGKIPGSKDCKAQNRAQEPMLEVGPRWLPPWTTLKLARLDLEDDFIDGLEKVLSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZZX8</accession>
    <name>A0AA38ZZX8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">PGG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_007408</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZZX8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026961">
        <property type="entry name" value="PGG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24186:SF18">
        <property type="entry name" value="ANKYRIN REPEAT FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24186">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13962">
        <property type="entry name" value="PGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="358"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="400"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="444"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="470"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="97"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="131"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="165"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="199"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="233"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="PGG" evidence="10">
        <location>
            <begin position="347"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9698311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="540" mass="59074" checksum="66EE889A52272006" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A113</accession>
    <name>A0AA39A113_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Bacterial surface antigen (D15) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_007184</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Plastid</location>
            <location evidence="3">Chloroplast outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9697948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A113"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009707">
        <property type="term" value="C:chloroplast outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009658">
        <property type="term" value="P:chloroplast organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009793">
        <property type="term" value="P:embryo development ending in seed dormancy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.310:FF:000013">
        <property type="entry name" value="Outer envelope protein 80 chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.50:FF:000006">
        <property type="entry name" value="Outer envelope protein 80, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.310:FF:000014">
        <property type="entry name" value="Outer envelope protein 80, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.310">
        <property type="entry name" value="membrane protein fhac"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.50">
        <property type="entry name" value="membrane protein fhac: a member of the omp85/tpsb transporter family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000184">
        <property type="entry name" value="Bac_surfAg_D15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039910">
        <property type="entry name" value="D15-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12815:SF32">
        <property type="entry name" value="OUTER ENVELOPE PROTEIN 80, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12815">
        <property type="entry name" value="SORTING AND ASSEMBLY MACHINERY SAMM50 PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01103">
        <property type="entry name" value="Omp85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0934">Plastid</keyword>
    <keyword evidence="1" id="KW-1002">Plastid outer membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bacterial surface antigen (D15)" evidence="5">
        <location>
            <begin position="370"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="105"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9697948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="673" mass="73034" checksum="AE65D4E1975852F6" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A1H2</accession>
    <name>A0AA39A1H2_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_007317</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A1H2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34468">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED FUTSCH-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34468:SF3">
        <property type="entry name" value="OS03G0288900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="65"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="105"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9698174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="239" mass="26783" checksum="B89F8E391D48E27E" modified="2024-01-24" version="1">MDLHSTQFAGKVQSLKSRTTGKNVRAGKNNSKDPPIAREKRAFGTSRSTNIPAKTETEKPAIKPTTGTTQKQSKSSRATAEEKKLPEKNKARLKKNSVCFQEKVAESPAKQHDDGPRTPAAKPKVSTPYHTAENCSKCRFDRLETATYWLAQIKLSESVGKHFVSAAFFRLALECKAEPIRNIRNELKGYLGRHGHLLGSNEWKDVNESYGLIENESSNVEQLHQELKHQLEEGKKSEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A1W0</accession>
    <name>A0AA39A1W0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_007468</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A1W0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832:SF228">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C 23-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="2">
        <location>
            <begin position="251"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="322"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="378"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="322"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="385"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9698399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="724" mass="80248" checksum="D47BEEA6A98AE849" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A3L4</accession>
    <name>A0AA39A3L4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Exostosin GT47 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_008075</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 47 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9699298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A3L4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010417">
        <property type="term" value="P:glucuronoxylan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004263">
        <property type="entry name" value="Exostosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040911">
        <property type="entry name" value="Exostosin_GT47"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11062">
        <property type="entry name" value="EXOSTOSIN HEPARAN SULFATE GLYCOSYLTRANSFERASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11062:SF229">
        <property type="entry name" value="GLUCURONOXYLAN GLUCURONOSYLTRANSFERASE IRX7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03016">
        <property type="entry name" value="Exostosin_GT47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="Exostosin GT47" evidence="8">
        <location>
            <begin position="97"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="441"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9699298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="460" mass="52780" checksum="1BA5E9C0DAF35667" modified="2024-01-24" version="1">MVDFSRPSRNRGFYVRMRLLHKQGRQEKSYCFRYFRWVLWLSLSLYFFSSYLITNHPRKLTAIPKVTVSNSKASRALFETVNTTIQQQPRDHQDLLKDLKVYIYDLPSKYNVDWLSNERCSNHLFASEVALHKALQESDVRTFDPWEADFFFVPVYVSCNFSTVNGFPAIGHARPLLASAIQHISTQLPFWNRSLGADHVFVASHDYGACFHAMEDVARADGIPEFLKKSIVLQTFGVKHQHPCQDVENVLIPPYVSPEKVQSTLDSAPANGQRDIWVFFRGKMEVHPKNISGRFYSKAVRTAIWQKYSGNRKFHLKRHRFAGYQSEIVRSVFCLCPLGWAPWSPRLVESVVLGCVPVIIADGIRLPFSEAIRWPEISLTVAEKDVGKLGMILDDVAATNLSTIQKNLWDPQNKRALLFNNQVQEGDATWQVLNALSHKLDRSHRRSRQSSNGHATRDVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A4J4</accession>
    <name>A0AA39A4J4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ENTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_006173</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Clathrin-coated vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A4J4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030125">
        <property type="term" value="C:clathrin vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03571">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276:SF95">
        <property type="entry name" value="ENTH_VHS FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276">
        <property type="entry name" value="EPSIN/ENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01417">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="5">
        <location>
            <begin position="26"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9700726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="325" mass="36684" checksum="B01C59ECA7455F7A" modified="2024-01-24" version="1">MGTLFINQIKRQAASFIQEKYRTARLALTDITPAELLAEEATNCDPIGPDAKTMTIIAEASFDIDDFWRIVDVLHKSKRRLFTIDWKQWRQSYKTLVLLDFLLTHGPEDFAEEFQCDSDVIEELGTFRYVDEKGFDWGILMQKKSENIIDLLRGGETLKEARFKALKITKEIQGFGNYMASPSSSSSSGSSRTSSFGSYSTSSSAWNDIEHELNKYERQSPTKDALGSYAHGGIYEDGKPTTFQQTNENFEAAHLWDCTSIQETGSLLDSEGEEDAKKDGFISGICSKFVGISPSRTNSEKATLRNYSDVGRVTKKKFHRQFSVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A4Q9</accession>
    <name>A0AA39A4Q9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">S-protein homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_006317</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the plant self-incompatibility (S1) protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A4Q9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060320">
        <property type="term" value="P:rejection of self pollen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010264">
        <property type="entry name" value="Self-incomp_S1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31232">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31232:SF8">
        <property type="entry name" value="S-PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05938">
        <property type="entry name" value="Self-incomp_S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 6" id="KW-0713">Self-incompatibility</keyword>
    <keyword evidence="5 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041485474" description="S-protein homolog" evidence="6">
        <location>
            <begin position="17"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9700926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="141" mass="16748" checksum="BA4C867EC6D018A5" modified="2024-01-24" version="1">MKRLTSFLLMFCFSLSIIFMSFRQSQFFSGSSYDVRVVNGFTDNSSLVLVIWCSSQHNDLGGRALQAGDDFSWSLRTNLWATTLFHCTMKWDQRRTSFEAFQVQRDSQRCRPFRTCFWLVKEDGFYFSNDQVNWKKDFSWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A538</accession>
    <name>A0AA39A538_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Vesicle-associated membrane protein 724</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_005738</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in the targeting and/or fusion of transport vesicles to their target membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endomembrane system</location>
            <topology evidence="8">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the synaptobrevin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A538"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14824">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15843">
        <property type="entry name" value="R-SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000004">
        <property type="entry name" value="Vesicle-associated membrane protein 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.50:FF:000009">
        <property type="entry name" value="Vesicle-associated membrane protein 724"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.50">
        <property type="entry name" value="Longin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010908">
        <property type="entry name" value="Longin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001388">
        <property type="entry name" value="Synaptobrevin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051097">
        <property type="entry name" value="Synaptobrevin-like_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21136">
        <property type="entry name" value="SNARE PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21136:SF72">
        <property type="entry name" value="VESICLE-ASSOCIATED MEMBRANE PROTEIN 724"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13774">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00219">
        <property type="entry name" value="SYNAPTOBREVN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01270">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50859">
        <property type="entry name" value="LONGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="194"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="Longin" evidence="11">
        <location>
            <begin position="10"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="12">
        <location>
            <begin position="130"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9700049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="221" mass="25148" checksum="E6D19E8BF231C6C5" modified="2024-01-24" version="1">MSQESFIYSFVARGTMVLAEYTEFTGNFPAIATQCLQRLPSANNKFTYNCDHHTFNFLVEDGYAYCVVAKESVGKQVSIAFLERLKADFKKRYGGGKADTATAKSLNKDFGPIMKEHMQYIIDHAEEIEKLLKVKAQVSEVKSIMLENIDKTLERGENLTILADKTEDLRSQAQQFKKQGSQVRRKMWFQNMKIKLVVLGILLILALVIWVSICHGFNCSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A5T2</accession>
    <name>A0AA39A5T2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_006680</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A5T2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015798">
        <property type="term" value="P:myo-inositol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015791">
        <property type="term" value="P:polyol transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17360">
        <property type="entry name" value="MFS_HMIT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000073">
        <property type="entry name" value="MFS myo-inositol transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050814">
        <property type="entry name" value="Myo-inositol_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020:SF12">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="28"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="69"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="103"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="126"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="159"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="187"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="272"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="310"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="341"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="374"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="410"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="437"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="32"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9701432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="499" mass="53161" checksum="A66FF04BFBE89DA9" modified="2024-01-24" version="1">MTIDSLPGSSGYLDSYPGRRITYFSNGYILGLTAVAGIGGLLFGYDTGVISGALLYIKDDFEVVGQSSFLQETIVSMALVGAMIGAAAGGWINDAYGRKKATLLADIVFTIGAIVMAAAPNPYVLIAGRLLVGLGVGVASVTAPVYIAEASPSEIRGGLVSTNVLMITGGQFLSYLVNLAFTEVPGTWRWMLGVSGVPSVIQFSLMLFLPESPRWLYLKGNKSQAISVLSKIYDPERLEDEIDQLAAAAEEERQRKNAVRYRDVFKSKEMRLAFLAGAGLQAFQQFTGINTVMYYSPTIVQMAGFHSNQLALLLSLIVAAMNAAGTIVGIYLIDHVGRRKLALSSLSGVFVSLVILSGSFYMQSSGSGSGLYGWLAVLGLALYIACFSPGMGPVPWAVNSEIYPEAYRGICGGMSATINWISNLIVAQSFLSVATAVGTGATFLILAGVAVLAFVFVIVFLPETKGLTFEEVDQLWKERARGHSSGEGLLENQDDNEAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A6A1</accession>
    <name>A0AA39A6A1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Chromatin modification-related protein MEAF6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_006153</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EAF6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A6A1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000123">
        <property type="term" value="C:histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015418">
        <property type="entry name" value="Eaf6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13476">
        <property type="entry name" value="CHROMATIN MODIFICATION-RELATED PROTEIN MEAF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09340">
        <property type="entry name" value="NuA4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="76"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="2"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="118"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="131"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9700693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="144" mass="15992" checksum="F5C3566A96BBAD5F" modified="2024-01-24" version="1">MLASLVGKRERLQDELRVIEKQVYEMETNYLQDSSHFGNVLKGFEGFLSSSKNTTNLKRSRKFQLEDRLFSLSSVTSPAAEELGVGRDDGRSDFGQGRSKGGSLPTNGQGKPKKGRTAPRDGKRIRPSSEADLDDEDDPDLSLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A9T3</accession>
    <name>A0AA39A9T3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Disease resistance RPP13-like protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_004755</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A9T3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056789">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766:SF40">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766">
        <property type="entry name" value="PLANT BROAD-SPECTRUM MILDEW RESISTANCE PROTEIN RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25019">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0611">Plant defense</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="7">
        <location>
            <begin position="19"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="6">
        <location>
            <begin position="176"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="8">
        <location>
            <begin position="431"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="domain" description="R13L1/DRL21-like LRR repeat region" evidence="9">
        <location>
            <begin position="694"/>
            <end position="818"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF25019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9703115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1279" mass="144225" checksum="67059FF31BA3D83D" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AA11</accession>
    <name>A0AA39AA11_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_004337</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Chloroplast inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AA11"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009706">
        <property type="term" value="C:chloroplast inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000150">
        <property type="entry name" value="Adenine nucleotide transporter BT1, chloroplastic/mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24089">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7" id="KW-0934">Plastid</keyword>
    <keyword evidence="7" id="KW-1001">Plastid inner membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 13" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="113"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="207"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="301"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9702554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="397" mass="42440" checksum="DA01C13410E3696F" modified="2024-01-24" version="1">MGRRGLQVFDDNRKGVFPNCDLGLQWGIQEGAFHPGGLFASVGQVGMGFGISPNSPNSRDNNNAGLKFPCMDLYVKYVSSPDGFKILGIPEAAEEGSSNKGKVGLKLKVKVANPSLRRLISGAIAGAVSRTAVAPLETIRTHLMVGSSGHSTTEVFNNIMKTDGWKGLFRGNLVNVIRVAPSKAIELFAYDTVNKNLSPKPGEQPKIPIPASLVAGACAGVSSTLVTYPLELLKTRLTIQGDVYNGLLDAFVKILQEGGPAELYRGLTPSLIGVVPYAATNYFAYDTLRKTYRKILKQEKIGNIETLLIGSLAGAISSSATFPLEVARKHMQVGALSGRQVYKNVLHALSSILEQEGIPGLYKGLGPSCLKLVPAAGISFMCYEACKRILVENEEDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AAP7</accession>
    <name>A0AA39AAP7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Increased DNA methylation 1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_005426</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9704130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AAP7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056511">
        <property type="entry name" value="IDM1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042163">
        <property type="entry name" value="PHF12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46309">
        <property type="entry name" value="PHD FINGER PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46309:SF14">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23209">
        <property type="entry name" value="IDM1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Increased DNA methylation 1 C-terminal" evidence="1">
        <location>
            <begin position="5"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF23209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9704130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="95" mass="11051" checksum="77E52BD84AA72C0E" modified="2024-01-24" version="1">MHECFEPIIEHHTKRDLIADIVYNSVSKFKRLDFRGFYIMALQKDDEFVCAATLRIHGHKVAEMPLVATAFKYRGQGMCQVLIHELEKVIFLNIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AB14</accession>
    <name>A0AA39AB14_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_005153</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AB14"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47747:SF3">
        <property type="entry name" value="OS03G0853600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47747">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P38-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="117"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9703745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="180" mass="20784" checksum="2A48DB03550E43B4" modified="2024-01-24" version="1">MVMEEKVEEEARALESESSVGLHPHGKLLSYYLGLSFSLFLALLPSSSSSISQLSTLHLKLLQVEQELCHLKSGRKEDSKANARVVKIFIGHREPWCREEKHMGDQIHAIAEEIAYLRAEVAELERFEAELKARVEELTREVGEREEMLHEPDKICLNFSLLFFSYFPHYFLRKIVGKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ADT4</accession>
    <name>A0AA39ADT4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Protein CHROMOSOME TRANSMISSION FIDELITY 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_003320</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acetyltransferase family. ECO subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ADT4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061733">
        <property type="term" value="F:protein-lysine-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028005">
        <property type="entry name" value="AcTrfase_ESCO_Znf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028009">
        <property type="entry name" value="ESCO_Acetyltransf_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45884">
        <property type="entry name" value="N-ACETYLTRANSFERASE ECO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45884:SF2">
        <property type="entry name" value="N-ACETYLTRANSFERASE ECO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13880">
        <property type="entry name" value="Acetyltransf_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13878">
        <property type="entry name" value="zf-C2H2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="N-acetyltransferase ESCO zinc-finger" evidence="11">
        <location>
            <begin position="110"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase ESCO acetyl-transferase" evidence="12">
        <location>
            <begin position="300"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="21"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF13878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF13880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9705200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="373" mass="41238" checksum="E14E8515B900463E" modified="2024-01-24" version="1">MQAKISSFFKPSSAPKSPPIFDDDDDADDETTPFSAMEEPPIFGDDNETTSFWEKEPKVVITYKRRAPNPDWEKSETDGGLIAETMKKPIVVDSSLPSRTLLNKKRSYAQFHLELGQSDFLLHSCSTCGLKYSPGDEGDEQVHKAFHKNYTHGIQFKGWRNERIIRIPSAGGGRIILVLDGDPPAQKNKVHEVVKMMEIELGGGWIFHKNCKVYLFISSQRVAGCLVAEPIKKAYKILSSSADERSNDTSSKEARPNSNTLQFGTVSFQREVIKRAPSVNSCEVLDGTPNGPVVCENEAVPATCGIRAIWVTPSNRRKHIASQLLDAVRKSFCMGFVLKSSQLAFSQPTSAGMALASNYFGSGSFLVYKTNKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AEB7</accession>
    <name>A0AA39AEB7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF7026 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_003494</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AEB7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054290">
        <property type="entry name" value="DUF7026"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22950">
        <property type="entry name" value="DUF7026"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7026" evidence="1">
        <location>
            <begin position="66"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9705477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="165" mass="18842" checksum="4E8873DC544FEB3A" modified="2024-01-24" version="1">MAMRVINILPPKGPKLHPPSAFFLPTNLPKPGPRSLIHCTNSISDAELSSDLATEVKKMNTHLVLRKEAMEKSREMLFTEMCQYLGMGSEEVKRRWRKMGEEEKRVLLKGFISEWGVNFHPLSAKSVKEMVEEYLHEDDPSPHSSSLSLLFPGLKRMFMVSQSNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AER8</accession>
    <name>A0AA39AER8_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_003216</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AER8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9705058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="72" mass="7262" checksum="EE35861B4B7AB409" modified="2024-01-24" version="1">MAAKAPTAIGFSSKVVSGNPSESDMRSTPSAMAWSIPANVSEAGGSYVSLEKSTLYMASRALGAMPVAVPLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AF28</accession>
    <name>A0AA39AF28_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">FAD-binding PCMH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_003792</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oxygen-dependent FAD-linked oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AF28"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.43.10:FF:000004">
        <property type="entry name" value="Berberine bridge enzyme-like 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.462.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="entry name" value="Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012951">
        <property type="entry name" value="BBE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32448">
        <property type="entry name" value="OS08G0158400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08031">
        <property type="entry name" value="BBE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041412322" description="FAD-binding PCMH-type domain-containing protein" evidence="8">
        <location>
            <begin position="23"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="9">
        <location>
            <begin position="74"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9705860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="551" mass="61727" checksum="04F883C1849FBF36" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AFH4</accession>
    <name>A0AA39AFH4_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_001586</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AFH4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047801">
        <property type="entry name" value="Peptidase_C45"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34180:SF1">
        <property type="entry name" value="BETA-ALANYL-DOPAMINE_CARCININE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34180">
        <property type="entry name" value="PEPTIDASE C45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9706085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="121" mass="13568" checksum="DE676A4F6685E91B" modified="2024-01-24" version="1">MKVQDENSISRQKRAAQLPKQSKDDFLSLLGDSEDAKYLIYMTGTLEDDTNTSSLSSLFFTILPHFSGPTLYTLCTAVLDLDKQALRNFSCILNIFKRVQDVAVKARGCLLQLCILKGLIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AFK7</accession>
    <name>A0AA39AFK7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Thaumatin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_001886</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thaumatin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AFK7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09217">
        <property type="entry name" value="TLP-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.110.10:FF:000003">
        <property type="entry name" value="Thaumatin I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.110.10">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037176">
        <property type="entry name" value="Osmotin/thaumatin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001938">
        <property type="entry name" value="Thaumatin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017949">
        <property type="entry name" value="Thaumatin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31048">
        <property type="entry name" value="OS03G0233200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00314">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002703">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00347">
        <property type="entry name" value="THAUMATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00205">
        <property type="entry name" value="THN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49870">
        <property type="entry name" value="Osmotin, thaumatin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00316">
        <property type="entry name" value="THAUMATIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51367">
        <property type="entry name" value="THAUMATIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041279033" description="Thaumatin-like protein" evidence="4">
        <location>
            <begin position="25"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="33"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="74"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="89"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="146"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="154"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="168"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="178"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR002703-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9706637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="225" mass="23977" checksum="19A9A5D00EF49B39" modified="2024-01-24" version="1">MGLCKILSISSFLLTTLFFTSSYAATFNIQNHCSYTVWAAAVPGGGMQLSSGQSWSLNVNAGTTGGRVWARTNCNFDASGNGKCETGDCGGILQCTAYGTPPNTLAEFALNQYSNLDFFDISLVDGFNVPMNFNPTSNGCTRGISCTADIVGQCPAELKTAGGCNNPCTVFKTDEYCCNSGSCSATTYSKFFKDRCPDAYSYPKDDQTSTFTCPAGTNYEVIFCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AFW8</accession>
    <name>A0AA39AFW8_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_001798</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AFW8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="21"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9706474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="516" mass="56789" checksum="4F140BE1FD97B547" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AG11</accession>
    <name>A0AA39AG11_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA-directed RNA polymerase III subunit RPC6</fullName>
            <shortName evidence="6">RNA polymerase III subunit C6</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_002081</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the eukaryotic RPC34/RPC39 RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AG11"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006383">
        <property type="term" value="P:transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000116">
        <property type="entry name" value="DNA-directed RNA polymerase III subunit RPC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007832">
        <property type="entry name" value="RNA_pol_Rpc34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016049">
        <property type="entry name" value="RNA_pol_Rpc34-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12780">
        <property type="entry name" value="RNA POLYMERASE III DNA DIRECTED , 39KD SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05158">
        <property type="entry name" value="RNA_pol_Rpc34"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028763">
        <property type="entry name" value="RNA_pol_Rpc34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR028763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9706941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="239" mass="26176" checksum="0C68C0FBB5D1EAE6" modified="2024-01-24" version="1">MSRSQGSSLKRKRPDTNTASQPLLDAERVLYDLIRSKEDMGIWTRDMKRETNLPDNVVTKSLKALQVKKLIKEVVNIQSKGRKHYMAVEFEPSKELTGGAWYVEGNLDTEYIKILKETCVKVLSKLKVATAEGISESINKTGLCATECSTQQIAEILRALVLDNEIMEVRSSGSGEFSSIPIGAVCYRCTNKGGLGGGGPTIGAMASIPCGVCPRITQCTPDGVISPKTCAYYTKWLDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AG73</accession>
    <name>A0AA39AG73_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_002160</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AG73"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="222"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="103"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9707043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="296" mass="33238" checksum="3A17DAEB2DB75026" modified="2024-01-24" version="1">MIDRYREDEDVAASKGKAPEDQSRGYTQKEEVVVSKKMWTTLFPPSFDRRQEIRRRSEPIFPAKPSSVGEARPLEEDLRSGPQLESEGTVRPLFRRPLRNRSSGEGTSSTSGVVELRRRSEPILLAKPSSVSEARPMEEDFGSGPQLEREGTASPLFRCLLPNRCSGEGTSLTSGVVALRNHHFEENKEGSKGRVGSDLCGFTVTGSPSSPKIRGKGPIFEGYVCLLLLSHPSLFLPLLVAWFFLCRVPLVQIFLVQSLFPSFLWKIELILKFCSIKTMLALFFKKMLVFLAVMRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AIS3</accession>
    <name>A0AA39AIS3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_002262</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AIS3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901259">
        <property type="term" value="P:chloroplast rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050502">
        <property type="entry name" value="Euk_RNA-bind_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025">
        <property type="entry name" value="OS02G0815200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025:SF6">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="89"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="188"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="166"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="266"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="170"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9707188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="288" mass="31740" checksum="606B351F3F780148" modified="2024-01-24" version="1">MAALEASLSIFSLYRSSSSKLLFSHRPPPSMSLHSSAFIPSICLKYPLYPLPLLYTNPTTKFSFELCSAVQEIEVEAKPEQTQEPNQKRKLFVLNLPWSLSVVDIKNLFGECGTVTDVEIIKQKDGRSRGYAFVTMASGEEAQAGVEKFDSYELSGRIIRVDFAKRFKKPSPPPPPPPAGPPAGETRHKLYVSNLAWKVRSTHLREFFSSNFNPVSVRVVFDSPSGRSGGYGFASFATKEEAEAVISALDGKELMGRPVHLKFSVKTIDESGDEKEGGDISEGQPEEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AK74</accession>
    <name>A0AA39AK74_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DYW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_000910</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. PCMP-H subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AK74"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000474">
        <property type="entry name" value="Pentatricopeptide repeat protein PPR986-12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:002121">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At1g59720, chloroplastic/mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000396">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At2g36730"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032867">
        <property type="entry name" value="DYW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046849">
        <property type="entry name" value="E2_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF508">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14432">
        <property type="entry name" value="DYW_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20430">
        <property type="entry name" value="Eplus_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="92"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="195"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="267"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="DYW" evidence="4">
        <location>
            <begin position="516"/>
            <end position="609"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9709173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="609" mass="68820" checksum="B200765862E9A82E" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2025-10-08" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AQ84</accession>
    <name>A0AA39AQ84_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">UvrD-like helicase ATP-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_001190</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000326">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045529">
        <property type="entry name" value="DUF6469"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039904">
        <property type="entry name" value="TRANK1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014016">
        <property type="entry name" value="UvrD-like_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21529">
        <property type="entry name" value="MAMMARY TURMOR VIRUS RECEPTOR HOMOLOG 1, 2 MTVR1, 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21529:SF4">
        <property type="entry name" value="TPR AND ANKYRIN REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20073">
        <property type="entry name" value="DUF6469"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00580">
        <property type="entry name" value="UvrD-helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51198">
        <property type="entry name" value="UVRD_HELICASE_ATP_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 5" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UvrD-like helicase ATP-binding" evidence="7">
        <location>
            <begin position="1086"/>
            <end position="1482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="2741"/>
            <end position="2807"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="2748"/>
            <end position="2758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="2792"/>
            <end position="2807"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="1107"/>
            <end position="1114"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9709589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="2807" mass="320446" checksum="1BAE1224B3222183" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D3X4</accession>
    <name>A0AA39D3X4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_026648</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D3X4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04873">
        <property type="entry name" value="ACT_UUR-ACR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045847">
        <property type="entry name" value="AIG1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45844:SF17">
        <property type="entry name" value="TRANSCRIPTION FACTOR BHLH131"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45844">
        <property type="entry name" value="TRANSCRIPTION FACTOR BHLH30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="7">
        <location>
            <begin position="55"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9670226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="239" mass="26622" checksum="75676CFCF85D0CCA" modified="2024-01-24" version="1">MHAFGNPEEEMQYFQSYHEFGSCSNQNNSLDGGPESQDAINDGNSNLETEARAMEARDKHRVAERDRRKRTNCHYSTLRNLLPNATKLPKASVLARVIRRLTELREAAAELWTRDEGDGTEELLFPGETNELRLGRCEGEGEGEVEGEGGVVKAMLNCEDRAELLSEVSTAVRSVGGRVVRAEMVTVGGWTKCLLWVHGLGQDGLEALRRALKVVVDRPSFFCGSGRGFRGNKRIRFSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D554</accession>
    <name>A0AA39D554_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_025798</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D554"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46241">
        <property type="entry name" value="ARMADILLO REPEAT-CONTAINING PROTEIN 4 ARMC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46241:SF1">
        <property type="entry name" value="OUTER DYNEIN ARM-DOCKING COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13646">
        <property type="entry name" value="HEAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ARM" evidence="3">
        <location>
            <begin position="343"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="2">
        <location>
            <begin position="385"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="448"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9672323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="471" mass="50966" checksum="E8D5B2EC88C0928B" modified="2024-01-24" version="1">MDQPEAKQQKMSLDWEESFNLFEEAVASESTAMQIQAIIKVAQLSNYAPDNVLTRAVPILTQLLGGSSSPLVQEAAAYCLKCIAHQGDRDDRLAKLIGQSGAIPITLRLLQHSYGGLQGVLVKFLWSIVTFGTHNRVIVVRNGGLEVIINMLGSCVGSTRRHLLEILSALVWLREVRRVITSLEGLQFLVEAIKFGGMASRERAAYAVGSLGVARRARPALVDLGAMQALMELLREGDVSAKLVAGNALGVISAHVDCIRPLAQAGAIPLYAELLRGAEPVGKEIAEDVFCVLAIAEVNAVSIAQHLAQILRENDDITKAAAAEILGHLSRYKHSVPFITNSGAIPVLVELLRQGSDEVKEKASGAIAQLSYNAGDRAALADAGAIPILMDLLGDDQPEELRNKAAEALISFSEDPSQRDRISEAFNIPSFQDIHNRLAQLLASDDNGEHMARPSRQMMSMEQLPWDPNLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D5N2</accession>
    <name>A0AA39D5N2_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_026195</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D5N2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041455291" evidence="1">
        <location>
            <begin position="19"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9672848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="58" mass="6546" checksum="65740DAEB33AFE08" modified="2024-01-24" version="1">MPLSQALWKLTEVGLLMALTPRPLPQPVPPQFRIHLHCAYHQGPGHETDRCTALRHAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D7Z4</accession>
    <name>A0AA39D7Z4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_023761</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D7Z4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21742">
        <property type="entry name" value="MobB_NDR_LATS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05599">
        <property type="entry name" value="STKc_NDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000042">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000106">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000102">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059233">
        <property type="entry name" value="MobB_NdrA/B/Cbk1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050839">
        <property type="entry name" value="Rho-assoc_Ser/Thr_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22988:SF76">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_135, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22988">
        <property type="entry name" value="MYOTONIC DYSTROPHY S/T KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="107"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="14">
        <location>
            <begin position="411"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="56"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9674411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="525" mass="60308" checksum="C2788EE8856E1FA8" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D8A4</accession>
    <name>A0AA39D8A4_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_024465</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="342"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="636"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="345"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="370"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="639"/>
            <end position="652"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9675548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1437" mass="156940" checksum="6DAD6E1B23C8DB1B" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DB65</accession>
    <name>A0AA39DB65_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phospholipid scramblase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_020670</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the phospholipid scramblase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DB65"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017128">
        <property type="term" value="F:phospholipid scramblase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005552">
        <property type="entry name" value="Scramblase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025659">
        <property type="entry name" value="Tubby-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23248:SF9">
        <property type="entry name" value="PHOSPHOLIPID SCRAMBLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23248">
        <property type="entry name" value="PHOSPHOLIPID SCRAMBLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03803">
        <property type="entry name" value="Scramblase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54518">
        <property type="entry name" value="Tubby C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9678563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="427" mass="48936" checksum="8660FB1BCE704B4A" modified="2024-01-24" version="1">MNSIKNWHRLAKNSKFTQEISKLHATCYLAVNRHLNGLQPQATMLEQAVLTRNVAWRSKLPSCLEGNFLRSNLSAISISRQFGQSARGDPQMDRDFLVQLWVVDRKAKGSRGKRKRKAVKYGADSKIVYGNQLSSQFPFGRWFSGASVTKEKPSEQEKPVLKQPPLSQSVTGFLEPASPEEARVAPLLARSNLLITRDIEWANLVLGFEQENRYAMVDVCYPQSPVGFIREQSNVIMRQLLRLRRPFVAYIVDAMGNELFRVRRPFWWITSSIYAEINGKEVGVVHRRWHLWRRVYDLYLGNKQFAAVENPGFWNWTFTLKDIDGKVLAEIDRDWRGFGFEIFTDAGQYVIRFGSSDPNSKSGPAKAIQELEVVRPLTLSERAVAVALAVSLDNDYFSRHGGCVRHLPFLSLHPLYFSLFKYKTCVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DC05</accession>
    <name>A0AA39DC05_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_022787</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9678010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DC05"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000660">
        <property type="entry name" value="RING/U-box superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15315:SF35">
        <property type="entry name" value="F21J9.10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15315">
        <property type="entry name" value="RING FINGER PROTEIN 41, 151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="2">
        <location>
            <begin position="156"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9678010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="252" mass="29595" checksum="B78AB838B8FC1E5A" modified="2024-01-24" version="1">MLDYQLARSSFRDSLKALEADIQHANFLAASIPRTKNGSWLQMKLAYNNLTPIFLFFIQWMNCSCTYLLPSYLNLVHIVIYKVHPERRLKISSYGRKATIREFYAVILPSLQHLHSYSSELDYAQEEDQSLQPVVRKRPEERKNLLDVDLEREDECGICLEPCTKMVLPNCCHMMCISCFRDWNTKSESCPFCRVSLKRVNSGDLWVLPCRDDVVDMETVSKEDVLHFYLYIHNLPKVIPDALFLMYYEFLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DC87</accession>
    <name>A0AA39DC87_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_020850</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DC87"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9678776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="205" mass="21903" checksum="CAB632C405BD27D9" modified="2024-01-24" version="1">MQVPLVASTSTVGDDPTEVIEARISETSETMLRLAMSLVRDVGALRSRRGGSEGNSSIPCPSTEHHTEDQPMRPTPVRQPSAEEIEPEDNLVPTPHSPVGMGPSPAPTYSEPLVPQSQAEASLPAEVTVVTVEDAQEGEGAGGSGSQAPKPSSMKRYKWTAKRIVKRPPACKSPFVAQCVKQFPKIPHADRVVADYALAEMGDPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DEK1</accession>
    <name>A0AA39DEK1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_019982</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; phospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DEK1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071618">
        <property type="term" value="F:lysophosphatidylethanolamine acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07991">
        <property type="entry name" value="LPLAT_LPCAT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045252">
        <property type="entry name" value="LPCAT1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23063:SF52">
        <property type="entry name" value="LYSOPHOSPHATIDYLCHOLINE ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23063">
        <property type="entry name" value="PHOSPHOLIPID ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="12" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="90"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="16">
        <location>
            <begin position="457"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="16">
        <location>
            <begin position="497"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="38"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9680830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="557" mass="63403" checksum="A9303FCCC0BF4B32" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DF48</accession>
    <name>A0AA39DF48_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">DNA repair protein RAD4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_018545</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the XPC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DF48"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000111">
        <property type="term" value="C:nucleotide-excision repair factor 2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071942">
        <property type="term" value="C:XPC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.20.110:FF:000002">
        <property type="entry name" value="DNA repair protein Rad4 family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.20.110">
        <property type="entry name" value="Rad4, beta-hairpin domain BHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.260.10">
        <property type="entry name" value="Transglutaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018327">
        <property type="entry name" value="BHD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004583">
        <property type="entry name" value="DNA_repair_Rad4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018325">
        <property type="entry name" value="Rad4/PNGase_transGLS-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018326">
        <property type="entry name" value="Rad4_beta-hairpin_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036985">
        <property type="entry name" value="Transglutaminase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12135:SF0">
        <property type="entry name" value="DNA REPAIR PROTEIN COMPLEMENTING XP-C CELLS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12135">
        <property type="entry name" value="DNA REPAIR PROTEIN XP-C / RAD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10403">
        <property type="entry name" value="BHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10404">
        <property type="entry name" value="BHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03835">
        <property type="entry name" value="Rad4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01030">
        <property type="entry name" value="BHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01031">
        <property type="entry name" value="BHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad4 beta-hairpin" evidence="7">
        <location>
            <begin position="625"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="domain" description="Rad4 beta-hairpin" evidence="8">
        <location>
            <begin position="678"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="99"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="353"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="103"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="365"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9682643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="755" mass="82654" checksum="8DA16C5085577CD0" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DFJ8</accession>
    <name>A0AA39DFJ8_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_018861</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DFJ8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050142">
        <property type="entry name" value="MADS-box/MEF2_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002487">
        <property type="entry name" value="TF_Kbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48019">
        <property type="entry name" value="SERUM RESPONSE FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01486">
        <property type="entry name" value="K-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00404">
        <property type="entry name" value="MADSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51297">
        <property type="entry name" value="K_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="7">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="K-box" evidence="8">
        <location>
            <begin position="46"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="80"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9683048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="205" mass="23423" checksum="DB19C2A44A8462B9" modified="2024-01-24" version="1">MGRGRVQLKRIENKISRQVTFSKRRSGLLKKAHEISVLCDAEVALIGNWSMDYPKLTARIEVLQRNLRHFVGEDLDPLSLRELQNLEQQLDTALKRIRTRKNQLMHESISELQKKEKSLVEQNNALAKKVKEKEKAEQNNRAQWEQQNNIGQNSSAFVVPPPPLQLPSLTIGKTELKLDLVPIPSCHHGCCATSMNKGWRSFPAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DFK6</accession>
    <name>A0AA39DFK6_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_017997</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9681895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DFK6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="166"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9681895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="192" mass="22345" checksum="D11FFF7275D4411C" modified="2024-01-24" version="1">MSRKEGGEKQSNMEGGSSCFSLHFGQIVPQLHLQNLHQQHHQQQQTIDYSRLTSFLEYDDSFLSVWNWKSIDDDDVNSSSHREESRPRYIEFQPGSLFNHDVFDLKLEYMKFEQLLEKASTSTKICDYEEVSNSTEINNYEEALSSKEIGDYEEVSSSTEINNYEEALSSKEIDDYEETSSSIETNDFKNED</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DIV2</accession>
    <name>A0AA39DIV2_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_017748</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DIV2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37748">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37748:SF1">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="13"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9685811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="62" mass="6465" checksum="2A81C9BD638F78EC" modified="2024-01-24" version="1">MVFSAIISCFLPSSSSSRVSDDAGGSGSKVPSMGKEKMSKSKSSGAPIVLSYFPLNSYLSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DJX9</accession>
    <name>A0AA39DJX9_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_017023</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DJX9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31656">
        <property type="entry name" value="ROOT CAP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041432124" evidence="1">
        <location>
            <begin position="26"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9684832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="276" mass="31255" checksum="39E3AE8E57A4C30A" modified="2024-01-24" version="1">MKTKLRGMCTLFLLILWFSASMTTSQPIKPCKVGCRGVRYSPCYMVWFPSPLRCPTSYFMDCMTCKPLCSCSLLGVICEDPRFVGRDGITFYFQSSKDQDFYLVSNANLHINARFMGKRNPNLGVTILVAARRTSTWENNIDHLTLSFDDKPISLLTNEGSKWLPPSFLIVSVMHTSEVNMVMIESHYIVVVPIIVHDSRVHGYNIIDEDCFDNLELGFKFYKLSSAMNGSKTKFGAPMLILGSLHKFSSSNDNVKEYANLQCNSGINEIGVLYRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DL56</accession>
    <name>A0AA39DL56_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">STAS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_014243</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DL56"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008271">
        <property type="term" value="F:secondary active sulfate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07042">
        <property type="entry name" value="STAS_SulP_like_sulfate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.750.24:FF:000002">
        <property type="entry name" value="Sulfate transporter 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="entry name" value="STAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018045">
        <property type="entry name" value="S04_transporter_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011547">
        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001902">
        <property type="entry name" value="SLC26A/SulP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00815">
        <property type="entry name" value="sulP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11814">
        <property type="entry name" value="SULFATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01740">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00916">
        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SpoIIaa-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01130">
        <property type="entry name" value="SLC26A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="66"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="123"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="151"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="181"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="236"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="260"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="312"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="352"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="383"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="411"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="443"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="domain" description="STAS" evidence="7">
        <location>
            <begin position="501"/>
            <end position="623"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9688481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="648" mass="71316" checksum="A265B8DE9643C06F" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DLS1</accession>
    <name>A0AA39DLS1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Hexosyltransferase</fullName>
            <ecNumber evidence="13">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_014919</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Golgi apparatus membrane</location>
            <topology evidence="2 13">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the glycosyltransferase 31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="13"/>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9689468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DLS1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.50:FF:000027">
        <property type="entry name" value="Hexosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002659">
        <property type="entry name" value="Glyco_trans_31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214:SF351">
        <property type="entry name" value="BETA-1,3-GALACTOSYLTRANSFERASE PVG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214">
        <property type="entry name" value="BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01762">
        <property type="entry name" value="Galactosyl_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10 13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12 13" id="KW-0464">Manganese</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9689468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="323" mass="37867" checksum="DE39B3E9DDE82165" modified="2024-01-24" version="1">MTMNPNFQQVVMKPTKHERSRFIIFSFFILFLCAVVSIYEVRSGNLLKFGRRTFFSYNASSSASLPPLNSTSDDIRIFIGILTLPDQYQRRHLLRIIYGTQSPAGAKVEVKFVFCNLTKEDQKVLVALEIMRYDDIIILNCTENMNQGKTYTYFSSLPEMLNSTEGPSPPYHYVMKADDDTYLRLDNLVESLRPLPREDLYYGCVVPCRSMDPFVHFMSGMGYLVSWDIVEWIRVSEIPKNHMVGPEDRVFADWLREGRRGRNRHMAKWAMYDYPEPWSVCTHELWPNTIGVHLLKKQEKWIETLKYFNVTQNLKPSKLYHIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DLT8</accession>
    <name>A0AA39DLT8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cyclin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_015538</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclin family. Cyclin U/P subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DLT8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013922">
        <property type="entry name" value="Cyclin_PHO80-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615:SF121">
        <property type="entry name" value="CYCLIN-U1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08613">
        <property type="entry name" value="Cyclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9686737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="220" mass="24633" checksum="E760FCADE5A945D3" modified="2024-01-24" version="1">MLVADDVNDHRIRPEMTGVESTTPRVLAVLAFAMERLVARNEGLVGESIGLMGKSLNVFHGVRAPSISIPKYLERIYKYTNCSPSCFVVGYVYIDRLVHKHPDSLVISLNVHRLLVTSVMVASKILDDVHYNNAFYARVGGVSNAELNRLELELLFMLDFGVVVSSRVFESYCFYLENEMLWNGAGQRMERAIVPNSVDEVTEISVEDTQTSSSPPQMVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DP49</accession>
    <name>A0AA39DP49_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Glutamate receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_012979</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DP49"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015276">
        <property type="term" value="F:ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19990">
        <property type="entry name" value="PBP1_GABAb_receptor_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000172">
        <property type="entry name" value="Glutamate receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000188">
        <property type="entry name" value="Glutamate receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044440">
        <property type="entry name" value="GABAb_receptor_plant_PBP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015683">
        <property type="entry name" value="Ionotropic_Glu_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001320">
        <property type="entry name" value="Iontro_rcpt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34836">
        <property type="entry name" value="OS06G0188250 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34836:SF9">
        <property type="entry name" value="RECEPTOR LIGAND BINDING REGION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00060">
        <property type="entry name" value="Lig_chan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="455"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="485"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="509"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="646"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="domain" description="Receptor ligand binding region" evidence="14">
        <location>
            <begin position="58"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor C-terminal" evidence="13">
        <location>
            <begin position="457"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="705"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="705"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="726"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="758"/>
            <end position="768"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9690595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="774" mass="86616" checksum="C48AF912E0649DC6" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DQR8</accession>
    <name>A0AA39DQR8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">PVL29_009502</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="22">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9693578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DQR8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000328">
        <property type="entry name" value="Leucine-rich repeat protein kinase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000383">
        <property type="entry name" value="Leucine-rich repeat receptor protein kinase EMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000129">
        <property type="entry name" value="Leucine-rich repeat receptor-like kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000453">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase HSL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45974:SF23">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45974">
        <property type="entry name" value="RECEPTOR-LIKE PROTEIN 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="14 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8 20" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041218855" description="non-specific serine/threonine protein kinase" evidence="20">
        <location>
            <begin position="24"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="532"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="597"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="871"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="625"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAJ9693578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="925" mass="101412" checksum="CCE06AEDD24C58C2" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DR57</accession>
    <name>A0AA39DR57_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_012011</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9693099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DR57"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9693099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="248" mass="29332" checksum="28093AD9C9933F6D" modified="2024-01-24" version="1">MVSRRLPERRSYAGEYRRIGDRRRIESLRVSENKRKKVSYPAHYRGRDTIRASIREYQTDRELNPCEYRRLQDRNDGIRASIGEEIKDEESNPCEYWRIKERKHPLRASIGEYQIDRESNPCEYRRIQDRNDGIRASTGEEIVFGRVPKSNRQTGNQSVRVLKNPRQKGCTGESKTEMMISGRVPEKRSYSGEYKRVTDRRGINPSEYCRIQDRKDGIRASTREEIVFGRVSRNRRKAKNRIRASTGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DS11</accession>
    <name>A0AA39DS11_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">PVL29_009824</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="24">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the leguminous lectin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DS11"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002229">
        <property type="term" value="P:defense response to oomycetes"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06899">
        <property type="entry name" value="lectin_legume_LecRK_Arcelin_ConA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000108">
        <property type="entry name" value="L-type lectin-domain containing receptor kinase S.4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000086">
        <property type="entry name" value="L-type lectin-domain containing receptor kinase S.4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000039">
        <property type="entry name" value="receptor-like protein kinase FERONIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050528">
        <property type="entry name" value="L-type_Lectin-RKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001220">
        <property type="entry name" value="Legume_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27007">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00139">
        <property type="entry name" value="Lectin_legB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0430">Lectin</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10 22" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041372921" description="non-specific serine/threonine protein kinase" evidence="22">
        <location>
            <begin position="21"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="349"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="379"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KAJ9694029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="680" mass="74696" checksum="4235547B7B62B836" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DS72</accession>
    <name>A0AA39DS72_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">mannan endo-1,4-beta-mannosidase</fullName>
            <ecNumber evidence="4">3.2.1.78</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_009942</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random hydrolysis of (1-&gt;4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.</text>
            <dbReference type="EC" id="3.2.1.78"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.78" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DS72"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016985">
        <property type="term" value="F:mannan endo-1,4-beta-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000012">
        <property type="entry name" value="Mannan endo-1,4-beta-mannosidase 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045053">
        <property type="entry name" value="MAN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31451">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31451:SF54">
        <property type="entry name" value="MANNAN ENDO-1,4-BETA-MANNOSIDASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26410">
        <property type="entry name" value="GH5_mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041434189" description="mannan endo-1,4-beta-mannosidase" evidence="8">
        <location>
            <begin position="29"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="9">
        <location>
            <begin position="72"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF26410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9694209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="471" mass="53209" checksum="05052DC66BFC6505" modified="2024-01-24" version="1">MDTYVGKLGSKIFFSVAIFMILFQSSSSTAVGVSGDEDLETLVENVADHLSYSSSNYGVYEMDDLGNEPWTMVQKKGNQFVVNDKPFYVNGFNTYWLMVFAVDQSTRGKVSEVFQQAASVGLTVCRTLAFNDGQWRALQKSPSVYDEEVFKGLDFVVSEARKYKIRLILSLINNWEGYGGKAQYVKWGKEAGLNLTSDDDFFSHPTLRSYYKANVKTVLNRVNTFTSITYKEDPTIFAWELMNEPRCTSDPTGNKLQSWIQEMAVYVKSMDPKHLLEIGLEGFYGPSTPDKVQFNPNTYAQQVGTDFIRNHLVLGVDFASVHIYPDSWISQSITDAHLDFTRSWMQAHIEDSEKYLGMPVVFAEFGVSSKDKGYNSSFRDTLISTVYKVVLNSTRKGGSGAGSLLWQLFPDGTDYMDDGYAIVLSKSPSTSNIIALQSTRLMMFNSMCARNCRWICHKKHPLDAFLFHDDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DSD2</accession>
    <name>A0AA39DSD2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UMP kinase</fullName>
            <ecNumber evidence="3">2.7.4.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Uridine monophosphate kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_010625</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; CTP biosynthesis via de novo pathway; UDP from UMP (UMPK route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UMP kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.22" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DSD2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033862">
        <property type="term" value="F:UMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006225">
        <property type="term" value="P:UDP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04254">
        <property type="entry name" value="AAK_UMPK-PyrH-Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000238">
        <property type="entry name" value="Aspartate/glutamate/uridylate kinase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1160.10:FF:000028">
        <property type="entry name" value="Uridylate kinase isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="entry name" value="Acetylglutamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01220_B">
        <property type="entry name" value="PyrH_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044822">
        <property type="entry name" value="Myb_DNA-bind_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015963">
        <property type="entry name" value="Uridylate_kinase_bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42833:SF1">
        <property type="entry name" value="UMP KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42833">
        <property type="entry name" value="URIDYLATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13837">
        <property type="entry name" value="Myb_DNA-bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="Carbamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb/SANT-like DNA-binding" evidence="9">
        <location>
            <begin position="192"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="Aspartate/glutamate/uridylate kinase" evidence="8">
        <location>
            <begin position="338"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="113"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="235"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="121"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="133"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00032092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9695228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="575" mass="62815" checksum="D3C6C4124C77DE6F" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DVD3</accession>
    <name>A0AA39DVD3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_010800</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DVD3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000007">
        <property type="entry name" value="Two-component response regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006447">
        <property type="entry name" value="Myb_dom_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046955">
        <property type="entry name" value="PHR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01557">
        <property type="entry name" value="myb_SHAQKYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31499">
        <property type="entry name" value="MYB FAMILY TRANSCRIPTION FACTOR PHL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31499:SF85">
        <property type="entry name" value="TRANSCRIPTION FACTOR MYB-RELATED FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Myb-like" evidence="5">
        <location>
            <begin position="116"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9695497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="195" mass="21859" checksum="E23EF9D430C10CA3" modified="2024-01-24" version="1">MRQKGHISTCSYTPRDFSQKVNIWIVSIAGKSEKGASSNDVPHLDNEDGMQIREALQLQLDLQKRLHEQYIEVFGYLCKSSPLALQAQCATSSSEGVSIASADPVSPVLRSKPRIRWTPDLHEHFVDCVNRLGGAETTPKAILKLMDSEGLTIFHVKSHLQKYRIAKYMPESAEGKPLFDVNSWTILYTCSDPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DX41</accession>
    <name>A0AA39DX41_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Trichome birefringence-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_007664</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PC-esterase family. TBL subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DX41"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016413">
        <property type="term" value="F:O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029962">
        <property type="entry name" value="TBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026057">
        <property type="entry name" value="TBL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025846">
        <property type="entry name" value="TBL_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285:SF14">
        <property type="entry name" value="PROTEIN PMR5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285">
        <property type="entry name" value="PROTEIN TRICHOME BIREFRINGENCE-LIKE 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13839">
        <property type="entry name" value="PC-Esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14416">
        <property type="entry name" value="PMR5N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041447721" description="Trichome birefringence-like N-terminal domain-containing protein" evidence="7">
        <location>
            <begin position="33"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="Trichome birefringence-like N-terminal" evidence="9">
        <location>
            <begin position="58"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="Trichome birefringence-like C-terminal" evidence="8">
        <location>
            <begin position="111"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF14416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9698710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="392" mass="44594" checksum="8D1B87725AA3ED45" modified="2024-01-24" version="1">MDFPSAPSSFYAFGSCFTFLVLLLVRPHIASSALLLGLKHHNNHHHNRRPFLQANQSTCALFMGTWVRDDAYPLYQFSDCPFIDAEFNCQMYGRPDTDYLKYRWKPTNCELPRFNGLEFLLRMKGKTVMFVGDSLGRNQWESLVCMISTAVPGSPTQIIRGDPLSTLKFLEYGVAVSFYRAPYLVDIEAVQGKRVLRLGDISGNGNAWRGVDVLSFNTGHWWSHKGSLQGWDYIEMDGTLYQDMDRLVAFEKGLRTWARWVDTNVDRTRTRVFFQSTSPTHYNPSEWSAGAVTTTKNCYGETAPMSGMTYPGAYPDQMRVVEEVMSQMSGPVYLLDITLLSEMRKDGHPSIYSGDLSPAQRANPDRSADCSHWCLPGLPDTWNQLFYTALFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DXE1</accession>
    <name>A0AA39DXE1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Isoflavone 2'-hydroxylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_006709</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DXE1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20653">
        <property type="entry name" value="CYP81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000023">
        <property type="entry name" value="Cytochrome P450 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050651">
        <property type="entry name" value="Plant_Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47947">
        <property type="entry name" value="CYTOCHROME P450 82C3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47947:SF62">
        <property type="entry name" value="CYTOCHROME P450, FAMILY 81, SUBFAMILY D, POLYPEPTIDE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11 12" id="KW-0349">Heme</keyword>
    <keyword evidence="8 11 12" id="KW-0408">Iron</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 12" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="11">
        <location>
            <position position="440"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9701471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="508" mass="57170" checksum="FCF33117ABD840A6" modified="2024-01-24" version="1">MEVSSLHSSLSLLFFFLLLVIGILFPRRRYGNLPPSPPAVPIIGHLHLLKQPVHRSLQRLSLKYGPIFSLRFGSQHVVIVSSPSAVEECFTKNDVVLANRPRLASGKYLGFNYTSMSTASYGEHWRNLRRLSALEIFSSSRLNMFLGIRRDEVKLLLLRLARDSREGFAKVELRPMLTELTFNIITRMVAGKRYYGEGVEFEEAKRFREIISEIFKLGGAPSNPTDFLPILRWIGFGDHEKKLKKTTRETKVILQGLIDEHRSGNDRGSVDNNSMIDHLLSLQKTEPEYYTDDIIKGLVLDLILAGTDTTAATMEWAMTLLLNHPDVLKKAKAELDIHVGKDRLIEESDLPKLRYLQSIISETLRVFPVAPLLVPHMSSDDCQIGGFDVPGGTLLLINAWAIHRDPQVWEDPTSFIPERYENGERENYKLLPFGIGRRACPGAGLAHRVVGLALGSLIQCCDWKKISKTTIDTTEGKGLTMPKLEPLEAMCKACEIIKTGSLELENNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DY74</accession>
    <name>A0AA39DY74_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Respiratory burst oxidase homolog protein H</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PVL29_007393</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Calcium-dependent NADPH oxidase that generates superoxide.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer and homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RBOH (TC 5.B.1.3) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DY74"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016174">
        <property type="term" value="F:NAD(P)H oxidase H2O2-forming activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd06186">
        <property type="entry name" value="NOX_Duox_like_FAD_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.80:FF:000026">
        <property type="entry name" value="Putative respiratory burst oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000049">
        <property type="entry name" value="Respiratory burst oxidase homolog A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000019">
        <property type="entry name" value="Respiratory burst oxidase homolog A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000778">
        <property type="entry name" value="Cyt_b245_heavy_chain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013112">
        <property type="entry name" value="FAD-bd_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013121">
        <property type="entry name" value="Fe_red_NAD-bd_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013623">
        <property type="entry name" value="NADPH_Ox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050369">
        <property type="entry name" value="RBOH/FRE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11972">
        <property type="entry name" value="NADPH OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11972:SF54">
        <property type="entry name" value="RESPIRATORY BURST OXIDASE HOMOLOG PROTEIN J-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08022">
        <property type="entry name" value="FAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08030">
        <property type="entry name" value="NAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08414">
        <property type="entry name" value="NADPH_Ox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00466">
        <property type="entry name" value="GP91PHOX"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01169">
        <property type="entry name" value="NADPH_oxidase_subgroup_(NOX)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="10" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="15 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0521">NADP</keyword>
    <keyword evidence="14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="292"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="410"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="448"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="637"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="19">
        <location>
            <begin position="168"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="20">
        <location>
            <begin position="502"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="27"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00055023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAJ9698287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="23">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="828" mass="94654" checksum="4E3E087B5D0FF0D4" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E080</accession>
    <name>A0AA39E080_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pre-rRNA-processing protein TSR2 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_005931</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TSR2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E080"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019398">
        <property type="entry name" value="Pre-rRNA_process_TSR2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21250">
        <property type="entry name" value="PRE-RRNA-PROCESSING PROTEIN TSR2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10273">
        <property type="entry name" value="WGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9700372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="112" mass="12617" checksum="0239597D085D231B" modified="2024-01-24" version="1">MESTNRNCVVASTQGGRLQDGISRHLSQWNGLQMAVQNQWGGRDSHQKSLQLAADIFSWFSESKAPLCVEDLENLLHESMLLSFNTEIEDGSIEQVAEELMIMHEDCQQGNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E1H4</accession>
    <name>A0AA39E1H4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Alpha-mannosidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_005157</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E1H4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004559">
        <property type="term" value="F:alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006013">
        <property type="term" value="P:mannose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1360:FF:000001">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.98.30:FF:000004">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.30">
        <property type="entry name" value="Golgi alpha-mannosidase II, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041147">
        <property type="entry name" value="GH38_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011682">
        <property type="entry name" value="Glyco_hydro_38_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050843">
        <property type="entry name" value="Glycosyl_Hydrlase_38"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11607">
        <property type="entry name" value="ALPHA-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11607:SF61">
        <property type="entry name" value="ALPHA-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17677">
        <property type="entry name" value="Glyco_hydro38C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07748">
        <property type="entry name" value="Glyco_hydro_38C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 38 C-terminal" evidence="2">
        <location>
            <begin position="14"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolases family 38 C-terminal" evidence="3">
        <location>
            <begin position="308"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF17677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9703749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="416" mass="46681" checksum="D497878BD8D15F55" modified="2024-01-24" version="1">MDSPHNESIEVGPGSLKMSFSSTTGQLERMFDSKRGVDLPIQQSYLWYGSSDGGLDSQPSSAYIFRPNGAPPTVVSRSVPLKVMRGPLVDEVHQQFSPWIYQVTRLYKDKDHAEVEFTIGPIPVDDSVGKEVITRMTANMVTNKVFYTDSSGRDFLKRVRDYREDWSLSVNQPEAGNYYPINLGIFTKDKKSEFSVLVDRATGGSSIKDGQVELMLHRRMIFYDSGGLGEALDEITCVENTCEGLTVRGNYYMSINLLGDGAQWRRTTGQEIYSPLLLAFTHEKLETWTASYLTKGTVMEPNYSLPLNVAVITLQELDDGSILLRLAHLYEAGEDAKYSTLAKVELQKMFRGKKIKEIKETNLSTNQEKSEMKSLKWKVEGDNGDETAPLRGGPVDNSTLVVELGPMEIRTFLLEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E3P5</accession>
    <name>A0AA39E3P5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Thaumatin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_000424</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E3P5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.110.10">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037176">
        <property type="entry name" value="Osmotin/thaumatin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001938">
        <property type="entry name" value="Thaumatin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31048">
        <property type="entry name" value="OS03G0233200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00314">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00347">
        <property type="entry name" value="THAUMATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00205">
        <property type="entry name" value="THN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49870">
        <property type="entry name" value="Osmotin, thaumatin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51367">
        <property type="entry name" value="THAUMATIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9708371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="167" mass="18102" checksum="D301E0755A3259F8" modified="2024-01-24" version="1">MIFSQLFSLKNTEENKRKRKGKRRGKFYPACGIRIPVFRSGWSGRFWGRTGCNFDGLGSCVTGNCGSRTIKCNSVGAAPPTTLVEFTLGTGGQDFYNVSLVHGCNLSMIVEGSGWSRMWASTGCTVDLNQGCPVELKVGDSNMCRSACEAFRSPEYSCSSAYSTPAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E457</accession>
    <name>A0AA39E457_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RCC1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_003864</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E457"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058923">
        <property type="entry name" value="RCC1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051625">
        <property type="entry name" value="Signaling_Regulatory_Domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22872">
        <property type="entry name" value="BTK-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25390">
        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="RCC1-like" evidence="4">
        <location>
            <begin position="60"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="106"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="164"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="216"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="268"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9705955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="329" mass="34945" checksum="1193DAFDCB12EA3C" modified="2024-01-24" version="1">MASCGFGAKAMVDAWALVTSTLLFCLRLIPIWIQFVLLLLVAFIRRLSARLAKFSHVCRGYGGFGALGHSVYHRELLPRMVEGSWNGKIRHIATAGTHTAAITESGELYTWGRDQGDGRLGLGPNCGPNEGGGLSIPSKVKALPVPVAAVSCGGFFTMVLTQEGQLWNWGANSNYELGRGDKVGGWKPQPIPSLQDVRIIQIASGGYHSLALTDDGKVLSWGYGQHGQLGHSSIENQKIPAVIEALADEHVIYIACGGSSSAAITDNGKLYMWGNAKDSQLGVPGLPETQPLPVEVKFLMEDDGLGPHNVLSVAVGASHAMCLVSRSGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YHI2</accession>
    <name>A0AA38YHI2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Metal transporter Nramp3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_026845</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NRAMP (TC 2.A.55) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YHI2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015086">
        <property type="term" value="F:cadmium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034755">
        <property type="term" value="P:iron ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000379">
        <property type="term" value="P:positive regulation of reactive oxygen species metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00221">
        <property type="entry name" value="NRAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001046">
        <property type="entry name" value="NRAMP_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01197">
        <property type="entry name" value="nramp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037982">
        <property type="entry name" value="Nramp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11706:SF109">
        <property type="entry name" value="METAL TRANSPORTER NRAMP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11706">
        <property type="entry name" value="SOLUTE CARRIER PROTEIN FAMILY 11 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01566">
        <property type="entry name" value="Nramp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00447">
        <property type="entry name" value="NATRESASSCMP"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="96"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="139"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="169"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="200"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="290"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="332"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="356"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="381"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="411"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="444"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="476"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9670558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="520" mass="57116" checksum="345D5F07DC4A43D5" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YJK4</accession>
    <name>A0AA38YJK4_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_025362</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YJK4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36808">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR ATRX-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36808:SF1">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR ATRX-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="105"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9671615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="123" mass="13375" checksum="99ED06513BA1E17B" modified="2024-01-24" version="1">MEVIHASTGLRKIKSEASGDIGDLESILRHSFRGVQMNAKTSAYQKDETEAVKQSSTSKAELVKINASKVDGTRAVGANQAVEQITMPTIRRESTHSSRNLGKIPDGRYGENETRASERGVIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YK43</accession>
    <name>A0AA38YK43_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Receptor-like serine/threonine-protein kinase</fullName>
            <ecNumber evidence="13">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PVL29_025524</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="13"/>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YK43"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048544">
        <property type="term" value="P:recognition of pollen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00028">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01098">
        <property type="entry name" value="PAN_AP_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000060">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.90.10.10:FF:000004">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000195">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.4.10:FF:000002">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.10">
        <property type="entry name" value="Bulb-type lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.4.10">
        <property type="entry name" value="Hepatocyte Growth Factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000858">
        <property type="entry name" value="S_locus_glycoprot_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024171">
        <property type="entry name" value="SRK-like_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32444:SF183">
        <property type="entry name" value="APPLE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32444">
        <property type="entry name" value="BULB-TYPE LECTIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08276">
        <property type="entry name" value="PAN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00954">
        <property type="entry name" value="S_locus_glycop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000641">
        <property type="entry name" value="SRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00473">
        <property type="entry name" value="PAN_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4 15" id="KW-0732">Signal</keyword>
    <keyword evidence="3 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041336098" description="Receptor-like serine/threonine-protein kinase" evidence="15">
        <location>
            <begin position="24"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="440"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="17">
        <location>
            <begin position="24"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="18">
        <location>
            <begin position="339"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="508"/>
            <end position="793"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR000641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ9671894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="818" mass="91990" checksum="D669B1E1E87EA01F" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YKU5</accession>
    <name>A0AA38YKU5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phase-change related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_025748</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YKU5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010800">
        <property type="entry name" value="GRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37389:SF16">
        <property type="entry name" value="GLYCINE-RICH CELL WALL STRUCTURAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37389">
        <property type="entry name" value="NODULIN-24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07172">
        <property type="entry name" value="GRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041425572" description="Phase-change related protein" evidence="2">
        <location>
            <begin position="27"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9672254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="88" mass="9383" checksum="480687FBD8643465" modified="2024-01-24" version="1">MAYSKTFLFVGLLFAVVLILSSEVSARELAETAETLINVEDEKHGGYGHGHDHEKGHGHNHEKGHGHGEPGHGGAGEDVEDETEGAKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YLK2</accession>
    <name>A0AA38YLK2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PLATZ transcription factor family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_026044</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YLK2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006734">
        <property type="entry name" value="PLATZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31065:SF56">
        <property type="entry name" value="OS11G0428700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31065">
        <property type="entry name" value="PLATZ TRANSCRIPTION FACTOR FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04640">
        <property type="entry name" value="PLATZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9672662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="109" mass="12341" checksum="2AEBD24D8D108164" modified="2024-01-24" version="1">MLVPPWVERFLSTRFYSNCTKHTDAAHKECNMYCIDCRSDAFCSHCRISLHSGNVVIQIRQSSHHNAVKVIEVEKLLDVSGVQSCVVNKFKVVYLDRNTQVKPSNGNAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YQ24</accession>
    <name>A0AA38YQ24_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Nucleobase-ascorbate transporter 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_023840</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YQ24"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006043">
        <property type="entry name" value="NCS2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037981">
        <property type="entry name" value="NCS2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11119">
        <property type="entry name" value="XANTHINE-URACIL / VITAMIN C PERMEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00860">
        <property type="entry name" value="Xan_ur_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="112"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="143"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="176"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="201"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="236"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="379"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="407"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="438"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="477"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="9"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9674539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="545" mass="59729" checksum="E1E418330893136F" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YS99</accession>
    <name>A0AA38YS99_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_024506</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YS99"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033612">
        <property type="term" value="F:receptor serine/threonine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000512">
        <property type="entry name" value="Leucine-rich repeat receptor-like serine/threonine-protein kinase BAM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000388">
        <property type="entry name" value="Leucine-rich repeat receptor-like tyrosine-protein kinase PXC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000454">
        <property type="entry name" value="Leucine-rich repeat receptor-like tyrosine-protein kinase PXC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001492">
        <property type="entry name" value="Phytosulfokine receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050647">
        <property type="entry name" value="Plant_LRR-RLKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056:SF17">
        <property type="entry name" value="LEUCINE-RICH REPEAT RECEPTOR PROTEIN KINASE EMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041421018" description="Protein kinase domain-containing protein" evidence="14">
        <location>
            <begin position="25"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="594"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="670"/>
            <end position="946"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9675617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="946" mass="102323" checksum="D8782B9B9F1067C3" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YU83</accession>
    <name>A0AA38YU83_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7950 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_021961</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9676700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YU83"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057710">
        <property type="entry name" value="DUF7950"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33595:SF7">
        <property type="entry name" value="OS12G0242500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33595">
        <property type="entry name" value="VON WILLEBRAND FACTOR A DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25821">
        <property type="entry name" value="DUF7950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7950" evidence="2">
        <location>
            <begin position="164"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9676700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="291" mass="32227" checksum="C8A5380D912F152F" modified="2024-01-24" version="1">MMMDAKGGCCIARYAGGAYDMSKVDRIMLRFRPIAPKPTSAGSVSGGSTLKSDEVYLRTGRGKRRYVRDNSKRCNRKRKASPEEKRDEIVGEAVVTLPLLPETPDRKDWSPPGQSDLLSVTKPQKNLPIWLSFANGSIEGHCGSDPAVNTDRTVVMPQPVRLVGSCVTVECVTDTWVDGDWLGSTDEEKINNLDRDTCPGFVSDGLNRVTWTNAAYRRMVGHVTPGPEMMVWLVMKARVPVTFPAITCRVRLQYRCGKEKSSLTLPCDVWRMDGGGFAWRLDVKAALSLGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YUJ8</accession>
    <name>A0AA38YUJ8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">CDP-diacylglycerol--serine O-phosphatidyltransferase</fullName>
            <ecNumber evidence="12">2.7.8.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Phosphatidylserine synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_022077</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes a base-exchange reaction in which the polar head group of phosphatidylethanolamine (PE) is replaced by L-serine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a CDP-1,2-diacyl-sn-glycerol + L-serine = a 1,2-diacyl-sn-glycero-3-phospho-L-serine + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16913"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
            <dbReference type="ChEBI" id="CHEBI:58332"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="2.7.8.8"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="12">Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from CDP-diacylglycerol: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Endoplasmic reticulum membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.8" evidence="12"/>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9676894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YUJ8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003882">
        <property type="term" value="F:CDP-diacylglycerol-serine O-phosphatidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106245">
        <property type="term" value="F:L-serine-phosphatidylethanolamine phosphatidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006659">
        <property type="term" value="P:phosphatidylserine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004277">
        <property type="entry name" value="PSS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15362">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15362:SF7">
        <property type="entry name" value="PHOSPHATIDYLSERINE SYNTHASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03034">
        <property type="entry name" value="PSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3 12" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8 12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 12" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="11 12" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="48"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="85"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="230"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="306"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="336"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="367"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="401"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU368094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9676894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="432" mass="50539" checksum="E2FF5FD05A5ACF60" modified="2024-01-24" version="1">MPFLIDMEPNGHRKMRRRNLIAQESSSLNLSEVIDELDPWTAWAYRPHTIKLLFIGTCFLIWASGALDPDNTSSGDLVTSVKRGIFAMIAVFLAYSLLQAPATVLIRPHPAIWRLVHGLAVVYLISLTFLLFQKRDDARQFMRFLHPNLGVELPERSYGSDCRIYVPENPSSRFKNVYETLFDEFVLAHIFGWWGKAIMIRNQPLLWLLSFGFELMELTFRHMLPNFNECWWDSIVLDVLVCNWFGIWAGMRTVRYFDGRTYEWVGISRQPNIISKVKRTLGQFTPAQWDKDEWHPLLGPWRFVQVLFLCIVFLTVELNTFFLKFCLWIPAQNPLIVYRLILWWLIAIPTIREYNSFLQDRTPAKKLGAFCWLSIAACIVELLICIKFGNGLYPNPMPRWLVTFWISVGVALVVFLIVWSWQLHKALEQKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YX80</accession>
    <name>A0AA38YX80_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Expansin-like EG45 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_022919</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9678177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YX80"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009664">
        <property type="term" value="P:plant-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007118">
        <property type="entry name" value="Expan_Lol_pI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002963">
        <property type="entry name" value="Expansin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007112">
        <property type="entry name" value="Expansin/allergen_DPBB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31867">
        <property type="entry name" value="EXPANSIN-A15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01225">
        <property type="entry name" value="EXPANSNFAMLY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50842">
        <property type="entry name" value="EXPANSIN_EG45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041299228" description="Expansin-like EG45 domain-containing protein" evidence="3">
        <location>
            <begin position="22"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="215"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like EG45" evidence="4">
        <location>
            <begin position="50"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9678177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="236" mass="25629" checksum="D0CD997ABD233E82" modified="2024-01-24" version="1">MVMGVVVYFASLISLFSLLQAKIPGIYSSGAWESARATFYGGSDASGAMGGVCGYGNLYSQGYGGNPVALSTVLSNHGLICGVYFRLKCANDPKPRHSGTPSILIVAASFYLPKLCSSPGKRPRYETLIGYLLLLPAQRAEYQAPTGPEARLVTGPTTPSATHIPMPSTLFPKPISKMKALRAIHGFFYYYFLVLESLYGSKIKWKTFGGPSLEHISLFSFLVLYAHTFSQIVFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YYU8</accession>
    <name>A0AA38YYU8_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_021051</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YYU8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010666">
        <property type="entry name" value="Znf_GRF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33680:SF1">
        <property type="entry name" value="OS05G0489500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33680">
        <property type="entry name" value="OS07G0190500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06839">
        <property type="entry name" value="Zn_ribbon_GRF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51999">
        <property type="entry name" value="ZF_GRF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GRF-type" evidence="7">
        <location>
            <begin position="95"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="GRF-type" evidence="7">
        <location>
            <begin position="162"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="6">
        <location>
            <begin position="263"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="6">
        <location>
            <begin position="297"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="65"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="65"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9679017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="322" mass="33567" checksum="9FC0A0494EC6ED8A" modified="2024-01-24" version="1">MAEPDDAVLVVEDEDFLSAVAAVEANALSNKRRRTYTSDAAKEGVYTAALRGSNSLAWKQSATAASGAPSSRGSFSVSSSGKSDGGDSSPPEKPCPCGLGTCLILTSNTQKNPGRKFYKCALREENGGCGFFQWCDNSSGISNDAFGAINSGLNSSFPDLKCPCGAGSCLILTAKTGNNAGQQFYRCPANQGGSCGFFKWCNDQTVPAGLPVSASRGYNTTTNASIKSYQTLPAGLPVGASRGYNTTTNASIKSYGAQSGSSCFKCGKEGHWAKDCQMSSPEPLANSGGRPASSGTCYKCGKPGHWARDCSSSQDTKVWQRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YYX2</accession>
    <name>A0AA38YYX2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_021127</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YYX2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000590">
        <property type="entry name" value="PR5-like receptor kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000178">
        <property type="entry name" value="serine/threonine-protein kinase PBS1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045874">
        <property type="entry name" value="LRK10/LRL21-25-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025287">
        <property type="entry name" value="WAK_GUB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27009">
        <property type="entry name" value="RUST RESISTANCE KINASE LR10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13947">
        <property type="entry name" value="GUB_WAK_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041429318" description="Protein kinase domain-containing protein" evidence="14">
        <location>
            <begin position="26"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="238"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="527"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="537"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9679097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="548" mass="62103" checksum="8137E1B86705AB88" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YZY5</accession>
    <name>A0AA38YZY5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_021654</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YZY5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000178">
        <property type="entry name" value="serine/threonine-protein kinase PBS1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045874">
        <property type="entry name" value="LRK10/LRL21-25-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27009">
        <property type="entry name" value="RUST RESISTANCE KINASE LR10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="62"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9679806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="310" mass="35054" checksum="78978534473468E6" modified="2024-01-24" version="1">MRNSFPFLAQDPLSKYYGLIYHAYCTSKSKKENQIKIESFLEDYKALKPSTYSYVDIKKITSKFKEKLGEGGYGAVYKGKLSNDILVAVKVLNNSKGNGEEFINEVGTISRIHHVNVVCLVGFCADGSKLVLIYEFLPNESLEKFIFSVAGENHSLGWEKLQHIAIGIAKGIEYLHQAKGTIGYIAPEVLSRNFGNASHKSDVYSFGMLLLEMVGGRKNIDVTVENIGQVYFLEWVYYHLNQGKELNIRVEKRGDTKIAKKLTIVGLWCIQWYPVDRPSMKVAVQMLEGEGDNLTMPPNPFASWFQQEQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z099</accession>
    <name>A0AA38Z099_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_021611</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z099"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000590">
        <property type="entry name" value="PR5-like receptor kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000178">
        <property type="entry name" value="serine/threonine-protein kinase PBS1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045874">
        <property type="entry name" value="LRK10/LRL21-25-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025287">
        <property type="entry name" value="WAK_GUB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27009">
        <property type="entry name" value="RUST RESISTANCE KINASE LR10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13947">
        <property type="entry name" value="GUB_WAK_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041236874" description="Protein kinase domain-containing protein" evidence="14">
        <location>
            <begin position="29"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="265"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="298"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="371"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="399"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9679753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="690" mass="78512" checksum="70FE346E3EF4E27F" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z0W3</accession>
    <name>A0AA38Z0W3_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_019372</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z0W3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9680067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="90" mass="10168" checksum="943B204782C0D7E8" modified="2024-01-24" version="1">MEEKGVFGIEAERLPQFEGTSEVRDCLARYGAVKANKLNRPRHELGEIVTEIRKGTHTQYLTYPSPSPLLSSSLLSNLPHLTNIFHYLVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z170</accession>
    <name>A0AA38Z170_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_019665</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z170"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF78">
        <property type="entry name" value="OS12G0587133 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="331"/>
            <end position="597"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9680404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1048" mass="120972" checksum="CDE7CF61B8801C88" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z2C1</accession>
    <name>A0AA38Z2C1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_020138</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SINA (Seven in absentia) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9681093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z2C1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16571">
        <property type="entry name" value="RING-HC_SIAHs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000041">
        <property type="entry name" value="E3 ubiquitin-protein ligase SINAT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.210.10:FF:000027">
        <property type="entry name" value="Pectinesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.210.10">
        <property type="entry name" value="Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018121">
        <property type="entry name" value="7-in-absentia-prot_TRAF-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052088">
        <property type="entry name" value="E3_ubiquitin-ligase_SINA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049548">
        <property type="entry name" value="Sina-like_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008974">
        <property type="entry name" value="TRAF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013010">
        <property type="entry name" value="Znf_SIAH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10315">
        <property type="entry name" value="E3 UBIQUITIN PROTEIN LIGASE SIAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10315:SF80">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SINAT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21362">
        <property type="entry name" value="Sina_RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03145">
        <property type="entry name" value="Sina_TRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21361">
        <property type="entry name" value="Sina_ZnF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51081">
        <property type="entry name" value="ZF_SIAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="11">
        <location>
            <begin position="22"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="SIAH-type" evidence="12">
        <location>
            <begin position="75"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9681093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="276" mass="31034" checksum="AAF417FDD8CB9D8A" modified="2024-01-24" version="1">MGNRVVPGDLHSLTIFEELLKCSVCFEYMNRPIYQCHNGHTLCSGCKARVLNKCPTCRQQLGDIRCLALEKMAESLQLHCKYEEFGCPEIIPYYTKLMHEDSCNFRPYSCPCSGWECSVVGDIPLLVSHLTDYHEAVMFNSCDFELGFLTEDLRENSGCRWIAAIINCYGKYFCVHTEACFHASTPICVVFLSLIGNQAEACNYSYSLEIGGNGRKLTFEGIPRSIRESERRSLESADSLIVLGSMALSLGGETREPKLEIRGRIWKSQIPVCTCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z4X4</accession>
    <name>A0AA38Z4X4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_018290</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z4X4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19145">
        <property type="entry name" value="AKR_AKR13D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050791">
        <property type="entry name" value="Aldo-Keto_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43625">
        <property type="entry name" value="AFLATOXIN B1 ALDEHYDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43625:SF81">
        <property type="entry name" value="OS01G0618100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="3">
        <location>
            <begin position="20"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9682340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="342" mass="37925" checksum="D760597C6C342A8D" modified="2024-01-24" version="1">MGEIQIPRVKLGNQGLEVSKLGFGCMGLSGIYNNPVPDDVGIAIIKHAFSKGITFFDTADAYGPFTNEVLIGKVLKELPREKIQLATKFGIAGFDLATGVTVNGTRKYVRSCCEASLKRLDVEYIDLYYQHRVDKSVPIEETMEELKKLVAEGKIKYIGLSEPSPDTIRRAHAVHPITALQMEWSLWTRDIEEEIVPLCRELGIGIVPYSPLGRGFFGGKVVGENAPANNFLRLNPRFQGENFEKNKNIYTKMEMLAEKHRCTPAQLALAWVLHQGDDVAPIPGTTKIKNLDDNISSLRLNLTKEVSKEISDAVPLTEVAGARAPDHFISTSWQFADTPARV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z4Y0</accession>
    <name>A0AA38Z4Y0_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_018171</name>
        <name evidence="2" type="ORF">PVL29_025791</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z4Y0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48434">
        <property type="entry name" value="(RAPE) HYPOTHETICAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48434:SF1">
        <property type="entry name" value="(RAPE) HYPOTHETICAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="197"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9672311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9682153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="249" mass="29809" checksum="F97E3BA9AF93F745" modified="2024-01-24" version="1">MNITDWNQKPFTSKTFSQPYHPMSYNYIDYMDAWYNMFYLQSYQHSWFIQFSRTCNFQFPAWFQKWWTFFGLLDSIFPPDIQEKFEFYKKRTSSQPFTQPKLLTFCSRLRIPWILTWNIIKKQEQPHPFPLSLSREFNIKWWDKFNHDFVCQQKVLELISARPGSSNSKDSSNSSQGPTQEEFLTLKSKCQASLAAATDLEQYRQELLQTLKQFDDQQENEDNEEIVISDDSYVDPEFILYGGPMGQAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z4Z1</accession>
    <name>A0AA38Z4Z1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">mitogen-activated protein kinase kinase kinase</fullName>
            <ecNumber evidence="2">2.7.11.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_018082</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.25" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z4Z1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004709">
        <property type="term" value="F:MAP kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd06606">
        <property type="entry name" value="STKc_MAPKKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000382">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050538">
        <property type="entry name" value="MAP_kinase_kinase_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016">
        <property type="entry name" value="MAP KINASE KINASE KINASE SSK2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016:SF56">
        <property type="entry name" value="MAPKK KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="61"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="546"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="94"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="583"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="609"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="624"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="651"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9682019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="671" mass="73662" checksum="B55CC61957736B99" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z4Z2</accession>
    <name>A0AA38Z4Z2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF4005 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_018278</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Binds to multiple calmodulin (CaM) in the presence of Ca(2+) and CaM-like proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IQD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z4Z2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025064">
        <property type="entry name" value="DUF4005"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32295:SF263">
        <property type="entry name" value="DUF4005 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32295">
        <property type="entry name" value="IQ-DOMAIN 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13178">
        <property type="entry name" value="DUF4005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4005" evidence="5">
        <location>
            <begin position="249"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="289"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="305"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="315"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9682322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="391" mass="43759" checksum="0D662C5EBC134EFE" modified="2024-01-24" version="1">MGKAGRWIRSLLGGKKEEKYKKKYGSFSGENVEISTAIVPRTPKEKRRWSFGHKSSRAFGSIVFPPLVNEGSPKQKNEQSFGMAVAVAATATAQTTTAETNGAGKHSVVEHAAATKIQAIFRSYLARKALCALRGLVKLQALVRGHLVRKQANTTLRRMHALMAIQVRARVQRIQVAEEAQIVVNRQSSVHRNFHQDNWLRIGLGEKMEVNQDGTQGDSNNRHGFLNYSQIQRIEQGIIKYYSGEVTPHHYSPMYRSNSTRKSFCFPQADCHADSSPHYPFLPNYMANTESSKAKARSQSEPKQRPKWSNKQKSRQTTLVGEASGSQHVQRQHPTSHAGSISIENPEPWLIKLYQSTMAIKDSECDSNSTVTSTSNYCTPAVAYEPHVTMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z6U0</accession>
    <name>A0AA38Z6U0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protease Do-like PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_019172</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S1C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z6U0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000012">
        <property type="entry name" value="protease Do-like 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000131">
        <property type="entry name" value="Protease Do-like 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041517">
        <property type="entry name" value="DEGP_PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046449">
        <property type="entry name" value="DEGP_PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001940">
        <property type="entry name" value="Peptidase_S1C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45980">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45980:SF18">
        <property type="entry name" value="PROTEASE DO-LIKE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17815">
        <property type="entry name" value="PDZ_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13365">
        <property type="entry name" value="Trypsin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00834">
        <property type="entry name" value="PROTEASES2C"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Protease Do-like PDZ" evidence="6">
        <location>
            <begin position="431"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="20"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="30"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="59"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="70"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF17815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9683481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="579" mass="63576" checksum="35FBF1094797D5BB" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z822</accession>
    <name>A0AA38Z822_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_019344</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z822"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31635">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31635:SF196">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="322"/>
            <end position="600"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9683728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="609" mass="71222" checksum="48BF8A555F4D6DA3" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z9Y0</accession>
    <name>A0AA38Z9Y0_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_016831</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the IQD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z9Y0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1190">
        <property type="entry name" value="iswi atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32295">
        <property type="entry name" value="IQ-DOMAIN 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32295:SF216">
        <property type="entry name" value="PROTEIN IQ-DOMAIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="319"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="391"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="179"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="18"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="28"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="319"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="336"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9684554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="481" mass="52914" checksum="E200F0E50253DEE2" modified="2024-01-24" version="1">MGKKGNWFSAVKKALSPEPKEKKDKTTPKSKKKWFGKHKNLDPVSSSTENAMPIPAPAPPVEDVKLTEAENEQSKHAYSVALATAVAAEAAVAAAHAAAEVVRLTSVTRFSGKSKEEVAAIRIQTAFRGYLARRALRALRGLVRLKSLIQGQSVKRQATTTLRCMQTLARVQSQIRARRIRMSEENQALQRQLQLKRDKELEKLRASMGDDWDDSIQSKEQLEANLQSKQEAAVRRERALAYAFSHQQTWKNSSKPANPTFMDPNNPHWGWSWLERWMAARPWESRSAMEKELISDHASVKSTTSRAISIGEISKAYARRDLNLDKKPSPTAQKLSRPPSRQSPSTPSKAQSSSSVTGKTKPASPKGSGWGADDDSRSMLSIQSERYRRHSIAGSSVRDDESLASSPAVPSYMASTESTRARSRLPSPLGLEKNGTPEKASGSSAKKRLSFPASPVGPRRHSGPPRVETSSIKAISPAHRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZAR6</accession>
    <name>A0AA38ZAR6_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_017424</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZAR6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9685385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="73" mass="8381" checksum="AAC263A6C61A4991" modified="2024-01-24" version="1">MGLQSLTSLKELRILLCPKLRSLVPKEGLPPTLAELKIEGCPILKKRCLKEKGKYWRKIAHIPYIDIDDIVQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZCE6</accession>
    <name>A0AA38ZCE6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PVL29_015294</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. Two pore calcium channel subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZCE6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000325">
        <property type="term" value="C:plant-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005245">
        <property type="term" value="F:voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000094">
        <property type="entry name" value="Two pore calcium channel protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000129">
        <property type="entry name" value="Two pore calcium channel protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000055">
        <property type="entry name" value="Two pore calcium channel protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044581">
        <property type="entry name" value="TPC1_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46988">
        <property type="entry name" value="TWO PORE CALCIUM CHANNEL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46988:SF2">
        <property type="entry name" value="TWO PORE CALCIUM CHANNEL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="5" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="14" id="KW-0407">Ion channel</keyword>
    <keyword evidence="12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="10" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="69"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="126"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="212"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="284"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="437"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="475"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="505"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="655"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="17">
        <location>
            <begin position="369"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="691"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="691"/>
            <end position="707"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ9686327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="738" mass="85835" checksum="84118DBC27517074" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZE31</accession>
    <name>A0AA38ZE31_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_015661</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZE31"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000041">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000213">
        <property type="entry name" value="Tyrosine-sulfated glycopeptide receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046956">
        <property type="entry name" value="RLP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48061:SF12">
        <property type="entry name" value="DISEASE RESISTANCE LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48061">
        <property type="entry name" value="LEUCINE-RICH REPEAT RECEPTOR PROTEIN KINASE EMS1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 12" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041453481" description="Leucine-rich repeat-containing N-terminal plant-type domain-containing protein" evidence="12">
        <location>
            <begin position="18"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="13">
        <location>
            <begin position="29"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="14">
        <location>
            <begin position="264"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9686892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="979" mass="108948" checksum="0B778D7A8BE2FFBE" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZEX5</accession>
    <name>A0AA38ZEX5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_016296</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZEX5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099402">
        <property type="term" value="P:plant organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.150.80:FF:000007">
        <property type="entry name" value="NAC domain-containing protein 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.80">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003441">
        <property type="entry name" value="NAC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036093">
        <property type="entry name" value="NAC_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31744:SF194">
        <property type="entry name" value="OS01G0888300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31744">
        <property type="entry name" value="PROTEIN CUP-SHAPED COTYLEDON 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02365">
        <property type="entry name" value="NAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101941">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51005">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NAC" evidence="7">
        <location>
            <begin position="21"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="173"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="317"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="177"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="317"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9687766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="377" mass="42755" checksum="69811D0B9D24B882" modified="2024-01-24" version="1">MAIAANMSQEDNKDEHEHDMVMPGFRFHPTEEELVEFYLRRKVEGKRFNVELITSLDLYRYDPWELPALAAIGEKEWFFYVPRDRKYRNGDRPNRVTTSGYWKATGADRMIRTENFRPIGLKKTLVFYSGKAPKGIRTSWIMNEYRLPQQETERSQKAEISLCRVYKRAGVEDHPSLPRSLPSRPSSSRGLQADKRQQDAAQHAIERFQAFGGQSQHVEMEKMIGESEGSSSTDVTTALGLSQQNAYGAIPALSATLAPPVPEEEDRLLLHHSKQGCSLVPPACTTLFTGSSSLPPHAVDDLHRLVNYQQASINQQQQYHNNPNQPSHQFSTLPVPQSSQSLALNTLPGSLQAAFSDRLWEWNPIPEASRDYNSPFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZFE9</accession>
    <name>A0AA38ZFE9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DYW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_016069</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. PCMP-H subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZFE9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:002148">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At2g29760, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000348">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032867">
        <property type="entry name" value="DYW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF540">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14432">
        <property type="entry name" value="DYW_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="129"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="230"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="261"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="366"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="DYW" evidence="5">
        <location>
            <begin position="581"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="22"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF14432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9687433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="673" mass="74356" checksum="698BB7E4CA6B76BD" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZGY7</accession>
    <name>A0AA38ZGY7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MATH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_014398</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00121">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.210.10">
        <property type="entry name" value="Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002083">
        <property type="entry name" value="MATH/TRAF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008974">
        <property type="entry name" value="TRAF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47242">
        <property type="entry name" value="TRAF-LIKE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47242:SF1">
        <property type="entry name" value="TRAF-LIKE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22486">
        <property type="entry name" value="MATH_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00061">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50144">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MATH" evidence="2">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="MATH" evidence="2">
        <location>
            <begin position="157"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="MATH" evidence="2">
        <location>
            <begin position="320"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="domain" description="MATH" evidence="2">
        <location>
            <begin position="484"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1278"/>
            <end position="1401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9688727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1553" mass="175598" checksum="483FD3A92F6D9B8D" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZI13</accession>
    <name>A0AA38ZI13_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_014917</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9689466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZI13"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9689466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="36" mass="4035" checksum="A96566B67747E6DC" modified="2024-01-24" version="1">MGKATKKSGRELGIGGWLYGLHTCREWIERKLMGNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZIG1</accession>
    <name>A0AA38ZIG1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Stigma-specific STIG1-like protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_012389</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the STIG1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9689676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZIG1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006969">
        <property type="entry name" value="Stig-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33227:SF21">
        <property type="entry name" value="F12F1.21 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33227">
        <property type="entry name" value="STIGMA-SPECIFIC STIG1-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04885">
        <property type="entry name" value="Stig1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9689676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="148" mass="16272" checksum="9ADD674ED72EABE2" modified="2024-01-24" version="1">MAAIRSVSSAEEEDFMFDLDQDDTITAEAENENENSDQLPLLESEQPISLRGTSRFLTEATRIKVTCDKYPTVCRAKGSAGKYCCKKKCVNVRTDKLNCGACGKKCRYSEICCKGECVNPSSDRRNCGGCGKKCKKGSFCVHGMCSYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZJ89</accession>
    <name>A0AA38ZJ89_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Cysteine-rich receptor-like protein kinase 29</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">PVL29_012644</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="23">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZJ89"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23509">
        <property type="entry name" value="Gnk2-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000129">
        <property type="entry name" value="cysteine-rich receptor-like protein kinase 10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000142">
        <property type="entry name" value="Cysteine-rich receptor-like protein kinase 10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.430.20:FF:000002">
        <property type="entry name" value="Cysteine-rich receptor-like protein kinase 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.430.20">
        <property type="entry name" value="Gnk2 domain, C-X8-C-X2-C motif"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002902">
        <property type="entry name" value="GNK2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038408">
        <property type="entry name" value="GNK2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002:SF1104">
        <property type="entry name" value="CYSTEINE-RICH RECEPTOR-LIKE PROTEIN KINASE 27-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002">
        <property type="entry name" value="RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD1-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01657">
        <property type="entry name" value="Stress-antifung"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51473">
        <property type="entry name" value="GNK2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="12 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 20" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041349025" description="Cysteine-rich receptor-like protein kinase 29" evidence="20">
        <location>
            <begin position="26"/>
            <end position="1189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="274"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="802"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="22">
        <location>
            <begin position="29"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="22">
        <location>
            <begin position="133"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="330"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="22">
        <location>
            <begin position="556"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="22">
        <location>
            <begin position="660"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="860"/>
            <end position="1137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="768"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="771"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="358"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="888"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAJ9690091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="25">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1189" mass="132573" checksum="0BD6BDB9BB71EEBE" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZJE0</accession>
    <name>A0AA38ZJE0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_012697</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZJE0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010019">
        <property type="term" value="P:chloroplast-nucleus signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031930">
        <property type="term" value="P:mitochondria-nucleus signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47936:SF5">
        <property type="entry name" value="PENTACOTRIPEPTIDE-REPEAT REGION OF PRORP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47936">
        <property type="entry name" value="PPR_LONG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="91"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="162"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="197"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="232"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="267"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9690171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="379" mass="42988" checksum="A81470072FEB07E9" modified="2024-01-24" version="1">MSSLARRLRAIFTSPLPATTKTPAKTIKRSSSNNSTLKEMVDKFKKSSDSKRFRSRYGYYEKAVLTLAKAKKFSFIEDILEHQKQYNEISTEVFAVRLITLYGKAGMFEHAHKLFDEMPKLNCERTVMSFNALLSACVNSKKFDKIDGFFQELPGNLGIVPDVVSYNIIVNAFCEMGSLDSALSVLDEMEKVGLEPDLITFNTLLNAFYQNGSYADGEKIWDLMKKNNVAPNVRSYNAKLRGLISENRMSEAVELIDEMKTSGIKPDVFTLSSLMKGFCNSGNLEEAKRWYSEIAKNELPSVRATYMTLIPFLVEKGDFEMATELCKEVFSRRWLIEPALLQQVLEGLVKESKIEAATELVELAKLKKFRITLELPSEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZKG9</accession>
    <name>A0AA38ZKG9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DC-UbP/UBTD2 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_013088</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZKG9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.225.20">
        <property type="entry name" value="Ub domain-containing protein, DC-UbP/UBTD2, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032752">
        <property type="entry name" value="DC-UbP/UBTD2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038169">
        <property type="entry name" value="DC-UbP/UBTD2_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039869">
        <property type="entry name" value="UBTD1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13609">
        <property type="entry name" value="UBIQUITIN DOMAIN CONTAINING 1 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16455">
        <property type="entry name" value="UBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DC-UbP/UBTD2 N-terminal" evidence="2">
        <location>
            <begin position="15"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9690753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="114" mass="12837" checksum="0145FD2979F23010" modified="2024-01-24" version="1">MGCTGSSRAKGDENSKKIRRPKPWKHSQPLTRTQLKQMRDEFWDTAPHYGGQKEIWDALRAATEADLTLAQAIVDSAGVIVQSADLTTCYDERGAKYELPKYVLSEPTNLIRDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZM82</accession>
    <name>A0AA38ZM82_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_013550</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C48 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZM82"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.395.10">
        <property type="entry name" value="Adenoviral Proteinase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="4">
        <location>
            <begin position="153"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9691400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="269" mass="30984" checksum="362BBDBD62A055C1" modified="2024-01-24" version="1">MAKSKESSKQYSLQKGVVPKENYTTVKGLFLRSSGKPLKMHEEGTKNDILSCERSLQDTKNMNVNEHYLHTLSNEKVCTEDLNEKNEDACATSIEVYMIPDENENIFPLPIKRSRRDYGKEPLRALCGYPDHVMKTRTSRERKPSKFKVSPFVHNLRKMVKREASEVEFCQTFFKLSDIGKDVFQFSIDWAPSIPTQENGWDCGVHVIRHMQRFKNGDSMTSSDFCNSIKIRQEIACDLVLHEGNREKHTIVAIVCTKTSTRAMKKLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZNP2</accession>
    <name>A0AA38ZNP2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PWWP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_011285</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZNP2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05162">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044679">
        <property type="entry name" value="PWWP2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000313">
        <property type="entry name" value="PWWP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33697">
        <property type="entry name" value="T17B22.17 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33697:SF1">
        <property type="entry name" value="TUDOR_PWWP_MBT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00855">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50812">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PWWP" evidence="2">
        <location>
            <begin position="16"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="318"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="427"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="715"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="443"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="482"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="721"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="745"/>
            <end position="754"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9692135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="794" mass="87137" checksum="FCD4567776231205" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZP75</accession>
    <name>A0AA38ZP75_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">DNA N(6)-methyladenine demethylase</fullName>
            <ecNumber evidence="13">1.14.11.51</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_011133</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>an N(6)-methyl-2'-deoxyadenosine in DNA + 2-oxoglutarate + O2 = a 2'-deoxyadenosine in DNA + formaldehyde + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:49524"/>
            <dbReference type="Rhea" id="RHEA-COMP:12418"/>
            <dbReference type="Rhea" id="RHEA-COMP:12419"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:90615"/>
            <dbReference type="ChEBI" id="CHEBI:90616"/>
            <dbReference type="EC" id="1.14.11.51"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:49525"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="14">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the alkB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.51" evidence="13"/>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9691873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZP75"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035516">
        <property type="term" value="F:broad specificity oxidative DNA demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141131">
        <property type="term" value="F:DNA N6-methyladenine demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035515">
        <property type="term" value="F:oxidative RNA demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035513">
        <property type="term" value="P:oxidative RNA demethylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.590:FF:000013">
        <property type="entry name" value="2-oxoglutarate-dependent dioxygenase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.590">
        <property type="entry name" value="Alpha-ketoglutarate-dependent dioxygenase AlkB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004574">
        <property type="entry name" value="Alkb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027450">
        <property type="entry name" value="AlkB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037151">
        <property type="entry name" value="AlkB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16557">
        <property type="entry name" value="ALKYLATED DNA REPAIR PROTEIN ALKB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16557:SF2">
        <property type="entry name" value="NUCLEIC ACID DIOXYGENASE ALKBH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13532">
        <property type="entry name" value="2OG-FeII_Oxy_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9 14" id="KW-0408">Iron</keyword>
    <keyword evidence="5 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="16">
        <location>
            <begin position="347"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="66"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="105"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="365"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="367"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="425"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00052047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00066586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR604574-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9691873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="457" mass="50223" checksum="65C007A96A4804C4" modified="2024-01-24" version="1">MNRGSARRAGGYRGRSSRSGVGTSLRYVPVGAGRHSTNDALECKDGATPNGREAPKQPSPGSISRQSEHELTLGHSYKQDRPSSGNNEGLSHMGINQHGQNPLESASSSSQPSGCPSASDSAHENHLPPLSDLSLKENTKNLKNESQIQTPIKYGINSMPFQNKGPQFEKNSLNKTIGTGNIELSEHPGVVEPFDICPVKTGTSIVLKGPLLSKNREKRRESKRAEEGLKGEVIRSGMVLLKGYISTSDQVKIVKKCRELGLGSGGFYQPGYRDGAKLNLKMMCLGKNWDPETGKYEDERPVDNAKPPPIPDEFCHLVKEAIQDSQALLSKEKIEASKVEKELPWMTPDICIVNFYTTSGRLGLHQDKDERQENLRKGLPVVSFSIGNSAEFLYSDQRDVFNADKVLLESGDVLIFGGKSRLIFHGVTSICPNTSPQVLLEETDLRHGRLNLTFRQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZPX2</accession>
    <name>A0AA38ZPX2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_012023</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9693111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZPX2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029472">
        <property type="entry name" value="Copia-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025724">
        <property type="entry name" value="GAG-pre-integrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054722">
        <property type="entry name" value="PolX-like_BBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039537">
        <property type="entry name" value="Retrotran_Ty1/copia-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057670">
        <property type="entry name" value="SH3_retrovirus"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648:SF22">
        <property type="entry name" value="REVERSE TRANSCRIPTASE TY1_COPIA-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648">
        <property type="entry name" value="TRANSPOSASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13976">
        <property type="entry name" value="gag_pre-integrs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22936">
        <property type="entry name" value="Pol_BBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14244">
        <property type="entry name" value="Retrotran_gag_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25597">
        <property type="entry name" value="SH3_retrovirus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="3">
        <location>
            <begin position="541"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="7"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9693111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="800" mass="92232" checksum="0B7FDB62AE0C3F6F" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZQC7</accession>
    <name>A0AA38ZQC7_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_012196</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9693333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZQC7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9693333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="90" mass="10410" checksum="821F8E9DDA9D2EF0" modified="2024-01-24" version="1">MDHFFTPDAEMVVQNRKSGRMNQTTINDAYKKEARERACMLIIRWMYEAAIPFNAVTYPSFQPMIEAIGQYGVGMKGPTLYEVRVTNLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZQI5</accession>
    <name>A0AA38ZQI5_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_012022</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9693110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZQI5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9693110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="34" mass="3873" checksum="E597A81CC7458F00" modified="2024-01-24" version="1">MKMSVKKTEMLVKYPAAFSNYPMSSSSKMEQQAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZQX5</accession>
    <name>A0AA38ZQX5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein DETOXIFICATION</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Multidrug and toxic compound extrusion protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_009547</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9693636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZQX5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13132">
        <property type="entry name" value="MATE_eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045069">
        <property type="entry name" value="MATE_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="146"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="183"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="212"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="244"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="287"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="318"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="361"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="405"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="434"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="464"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9693636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="518" mass="57551" checksum="8361BFBEA8B20AD8" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZRT5</accession>
    <name>A0AA38ZRT5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rhamnogalacturonase A/B/Epimerase-like pectate lyase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_012095</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9693208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZRT5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004650">
        <property type="term" value="F:polygalacturonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000046">
        <property type="entry name" value="Polygalacturonase QRT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039279">
        <property type="entry name" value="QRT3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024535">
        <property type="entry name" value="RHGA/B-epi-like_pectate_lyase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33928:SF4">
        <property type="entry name" value="PECTATE LYASE SUPERFAMILY PROTEIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33928">
        <property type="entry name" value="POLYGALACTURONASE QRT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12708">
        <property type="entry name" value="Pect-lyase_RHGA_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041402505" description="Rhamnogalacturonase A/B/Epimerase-like pectate lyase domain-containing protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="Rhamnogalacturonase A/B/Epimerase-like pectate lyase" evidence="3">
        <location>
            <begin position="71"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9693208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="473" mass="51170" checksum="9D8E51B6E0694E2F" modified="2024-01-24" version="1">MARGDLLIKLFVSLCFIFGHGESSYAGVGSYDDQILQMASIKASFLKRDVISPSRSHTIQPISSSSREHHATDYGADPTGRSDSTEALQQAIFDAFASPIDGHLMQGITNLGGVELHLDGGTYMISRPLRLPDIGGGNFMIHGGSLRASSDFPTDRHLIELSSSSKSLSYEYITLKDLMLDSNFRGGGIAVINSIRTTIDNCYISHFTTNGILIQGGHETYLRSSFIGQHITVGSDPREKDFSGIGINIVGNDNAVTDVVVFSAAIGVLIEGQANVLTGVHCYNKATSFGGVGIYLKSPGFTQNRILNCYLDFTGVVMDDPVQVQISNSFFLGNAYISIKSLKGIVEGVSIVHNMFSGDYTGVPIVQLDQSNEPFTLIDQVIVDHNNVRGMRLRSTVARGSTWGNGTTWTVDFSKVLLFPNLIKNVQYALQAGKLFPKHVLRNLSGNAVTVESDVPVSATLHVVVDQSMDGYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZTZ7</accession>
    <name>A0AA38ZTZ7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3-like 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_010031</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family. RAD16 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZTZ7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008094">
        <property type="term" value="F:ATP-dependent activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016818">
        <property type="term" value="F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16509">
        <property type="entry name" value="RING-HC_HLTF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014905">
        <property type="entry name" value="HIRAN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050628">
        <property type="entry name" value="SNF2_RAD54_helicase_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626:SF17">
        <property type="entry name" value="HELICASE-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08797">
        <property type="entry name" value="HIRAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00910">
        <property type="entry name" value="HIRAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
        <location>
            <begin position="256"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="13">
        <location>
            <begin position="640"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="15">
        <location>
            <begin position="714"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="315"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="317"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9694333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="884" mass="99208" checksum="CD048F84A9C2B482" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZUV2</accession>
    <name>A0AA38ZUV2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_010944</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Endomembrane system</location>
            <topology evidence="12">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZUV2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11393">
        <property type="entry name" value="bHLH_AtbHLH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01868">
        <property type="entry name" value="Rab11_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000274">
        <property type="entry name" value="ras-related protein RABA5a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000021">
        <property type="entry name" value="Transcription factor bHLH130 family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045239">
        <property type="entry name" value="bHLH95_bHLH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-0636">Prenylation</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="15">
        <location>
            <begin position="327"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="103"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="367"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="103"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9695718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="596" mass="65323" checksum="6D6DDE285BCD64E8" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZWC3</accession>
    <name>A0AA38ZWC3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_008400</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZWC3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071244">
        <property type="term" value="P:cellular response to carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902456">
        <property type="term" value="P:regulation of stomatal opening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009637">
        <property type="term" value="P:response to blue light"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010114">
        <property type="term" value="P:response to red light"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001659">
        <property type="term" value="P:temperature homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd13999">
        <property type="entry name" value="STKc_MAP3K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000034">
        <property type="entry name" value="Kinase suppressor of Ras 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000310">
        <property type="entry name" value="Serine/threonine-protein kinase HT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF271">
        <property type="entry name" value="ATMRK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="99"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="16"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="30"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9696140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="406" mass="45141" checksum="FBA562126B4767A7" modified="2024-01-24" version="1">MKMDLRSDDEVLVSAKAEISRTGEGETVSDKSSISSSDLGSKKDGGNTSNKDLFLRADKIDFKSWDIQLDKHLSRVISRDREVNTNTKKEDWEIELSKLDIRSVIAHGTYGTVYRGVYDGQDVAVKVLDWGEDGLATAAETAALRTSFRQEVAVWHKLDHPNVTKFIGASMGTSDLRIPSNSISSDGRNPVPSRACCVVVEYLPGGTLKKFLIRNRRKKLAFKIVIQLALDLSRGLSYLHSKKIVHRDVKTENMLLDVHRTLKIADFGVARVEAQNPRDMTGETGTLGYMAPEVLDGKPYNRKCDVYSFGICLWETYCCDMPYPDLSFADISSAVVRQNLRPDIPRCCPSSLASIMRKCWDGNPDKRPDMDEVVRLLEAIDTSKGGGMIPEDQASSCFCFSVARGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZY00</accession>
    <name>A0AA38ZY00_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Acyl-[acyl-carrier-protein] hydrolase</fullName>
            <ecNumber evidence="11">3.1.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_009285</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Plays an essential role in chain termination during de novo fatty acid synthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Plastid</location>
            <location evidence="1 11">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the acyl-ACP thioesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.-" evidence="11"/>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZY00"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000036">
        <property type="term" value="F:acyl carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016297">
        <property type="term" value="F:fatty acyl-[ACP] hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00586">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.129.10:FF:000014">
        <property type="entry name" value="Acyl-[acyl-carrier-protein] hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049427">
        <property type="entry name" value="Acyl-ACP_TE_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002864">
        <property type="entry name" value="Acyl-ACP_thioesterase_NHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045023">
        <property type="entry name" value="FATA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31727">
        <property type="entry name" value="OLEOYL-ACYL CARRIER PROTEIN THIOESTERASE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31727:SF6">
        <property type="entry name" value="OLEOYL-ACYL CARRIER PROTEIN THIOESTERASE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01643">
        <property type="entry name" value="Acyl-ACP_TE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20791">
        <property type="entry name" value="Acyl-ACP_TE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="10 11" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="7 11" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="6 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 11" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9 11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5 11" id="KW-0934">Plastid</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Acyl-ACP thioesterase N-terminal hotdog" evidence="13">
        <location>
            <begin position="89"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-ACP thioesterase-like C-terminal" evidence="14">
        <location>
            <begin position="252"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="314"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF20791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9697395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="376" mass="42514" checksum="4D555784BE6D73D3" modified="2024-01-24" version="1">MSVSMLKAPCNAADQLQALAQCRAHAAPLRLPRRRISGVSFSAPPRAPVVAVLDRVAAADGEQSRLAAAGSGNLADRLRLGSLTEDRLSYKERFIVRCYEVGINKTATVETIANLLQEVGCNHAQSVGFSTDGFATTHTMRKLHLIWVTARMHIEIYKYPAWSDVIEIETWCQGEGRIGTRRDWILKDYATGQVIGRATSKWVMMNQDTRRLQKVSDDVRDEYLIYCPRELRLAFPEENNSSLRKISKLEDPSQYYRLGLVPRRADLDMNQHVNNVTYIGWVLESMPQEIIDTHELQTITLDYRRECQHDDIVDSLTSPEPTEDAKTIPELQGTNGSATATKGQQDCCQFLHLLRLSGEGLEINRGRTEWRKKTAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A1W9</accession>
    <name>A0AA39A1W9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_008097</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9699332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A1W9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010447">
        <property type="term" value="P:response to acidic pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010044">
        <property type="term" value="P:response to aluminum ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000145">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044300">
        <property type="entry name" value="STOP1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058196">
        <property type="entry name" value="zf-C2H2_STOP1/2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059161">
        <property type="entry name" value="Znf-C2H2_STOP1/2_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46352">
        <property type="entry name" value="PROTEIN SENSITIVE TO PROTON RHIZOTOXICITY 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46352:SF8">
        <property type="entry name" value="PROTEIN SENSITIVE TO PROTON RHIZOTOXICITY 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23115">
        <property type="entry name" value="zf-C2H2_STOP2_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23118">
        <property type="entry name" value="zf-C2H2_STOP2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="223"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9699332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="390" mass="43014" checksum="088FC5134920E8F3" modified="2024-01-24" version="1">MIPGGTSNCFVNGSQGLQMYGMTAEGSVSSSLGGNSVLEAHSCSLVYSFSILKDKVHQVQSLISIFVPPNQGQPESMAMAVAGMGNLIQEVIAAASSMMYNCQQMGYGAAPGNSGTNHGLPQQGVELSDGRVCGNTGVVQMGEERGQGFYSSDQSLDWYGDNHNNSNTNDHSRTIIVSNNEKVESRELPRGSTQMNEGLGGVLPKTFDIVELDAEDLLAKYTHYCQICGKGFKRDANLRMHMRAHGDEYKSNAALSNPMKNNGSEMENKDDLIKLPRKYSCPQEGCRWNRKHAKFQPLKSMICVKNHYKRSHCPKMYICKRCNQKQFSVLSDLRTHEKHCGDLKWLCSCGTTFSRKDKLMGHVALFVGHTPAINSLSKPVKFHQNASRLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A374</accession>
    <name>A0AA39A374_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_005729</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A374"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9700037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="65" mass="7293" checksum="16CABF3AE593CEC6" modified="2024-01-24" version="1">MVKEAVDRVTDDYVKSAIDWMEVYKGIPATCNGNFYIFQLGGNCLLVSLILGLESLFMGGRSQWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A6M5</accession>
    <name>A0AA39A6M5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Retrovirus-related Pol polyprotein from transposon TNT 1-94</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_006704</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A6M5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025724">
        <property type="entry name" value="GAG-pre-integrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054722">
        <property type="entry name" value="PolX-like_BBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47592">
        <property type="entry name" value="PBF68 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47592:SF31">
        <property type="entry name" value="ZINC FINGER, CCHC-TYPE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13976">
        <property type="entry name" value="gag_pre-integrs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22936">
        <property type="entry name" value="Pol_BBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="216"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase catalytic" evidence="4">
        <location>
            <begin position="438"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="157"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="157"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9701465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="540" mass="61607" checksum="F4DAE5F1B9F7788B" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A7G5</accession>
    <name>A0AA39A7G5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BURP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_004218</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A7G5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044816">
        <property type="entry name" value="BURP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004873">
        <property type="entry name" value="BURP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31236:SF2">
        <property type="entry name" value="BURP DOMAIN PROTEIN RD22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31236">
        <property type="entry name" value="BURP DOMAIN PROTEIN USPL1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03181">
        <property type="entry name" value="BURP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01045">
        <property type="entry name" value="BURP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51277">
        <property type="entry name" value="BURP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BURP" evidence="2">
        <location>
            <begin position="87"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9702381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="269" mass="30222" checksum="0063B0D3D275CE09" modified="2024-01-24" version="1">MIKEVVAVSHASLPSEDYWKLALPNTAMPRAVRDLLLPNSMEDGSSINASEIVLNVFPGNDSVQYQNPSAAETPTEDRPQDTSRKGYFLEKDLHSSTKMKMDFRKTTNRALFLPHQIADSIPFSSDKLPEILSRFSLKQDSEEAEIMKETIQECEQPALEGDSRFCATSLESLVDFSISELGKNIKAISTEVEMGSQEYRLGVEVKVVADKSVLCHKQKCTHTLCFTATQSTTQGFTHFHLWEPRMEPKLKLWLLAISIHRLGTQACNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A7R1</accession>
    <name>A0AA39A7R1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_004155</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A7R1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000131">
        <property type="entry name" value="Monoglyceride lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051044">
        <property type="entry name" value="MAG_DAG_Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11614">
        <property type="entry name" value="PHOSPHOLIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="1">
        <location>
            <begin position="133"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9702274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="399" mass="44603" checksum="B667F22AD907EC53" modified="2024-01-24" version="1">MESVQMEELTSGASGRIIPVFKNLRRSVLSWQSIRRSLIFIHSIFLWLILLLPRHRLSSPAQSPPAPVKSCRRRSVFRRDEEDALKRRALAEGLEMVTESEDGTSLCRCATSLFYGTRRNALFCRSWFPVAGEMKGIMIIIHGLNEHSGRYADFAKQLTSRSFGVYAMDWIGHGGSDGLHGYVPSLDHVVADTGAFLEKIKSENPGIPCFLFGHSTGGAVVLKAASYPEIEGILEGIVLTSPALRVKPAHPIVGAVAPIFSLVVPRFQFKGANKRGIPVSRDPAAMLAKYSDPLVYTGPIRVRTGHEILRISSYLTRNFKSVTVPFFVLHGTADRVTDPLASQDLYTEAASRCKNIKLYDGFLHDLLFEPEREEIAQDIIDWMEKRLCGHDFENVHSHW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A821</accession>
    <name>A0AA39A821_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_004439</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A821"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35711:SF1">
        <property type="entry name" value="ECTODERMAL, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35711">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9702708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="175" mass="19205" checksum="0B7D624BC52E3800" modified="2024-01-24" version="1">MGTLPLNAVVLKDGFASSLMEAVVAETAKSLSCILFMMGSLLNGMVLLSMGPATFEWFPIPKHHGLKQSDPENKDASDTEDDEEDDDDADDQDDDDDQDDLSGEEGNNKGDGDPEDVPQANGDGASEDDEDDDDDDDDDDDEDDDDNDSDDGDEEREEEEDEEDEIPQPPTKRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AAA5</accession>
    <name>A0AA39AAA5_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_004943</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Cold-regulated 413 protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AAA5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008892">
        <property type="entry name" value="COR413"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33596">
        <property type="entry name" value="COLD-REGULATED 413 PLASMA MEMBRANE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33596:SF4">
        <property type="entry name" value="COLD-REGULATED 413 PLASMA MEMBRANE PROTEIN 4-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05562">
        <property type="entry name" value="WCOR413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="67"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="95"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="133"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="164"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9703362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="190" mass="20319" checksum="C12C46E087709941" modified="2024-01-24" version="1">MKIVDTVAIAHALALAAYADAASAEQSSSQQTFSSSTTGGRAAFQWGGTISATFLLILNQVGRRSSIQSSLLVLFLLTSFPTLLLNIVRGQIGRWFAFLAVAANLFFPQKFPVAGFILLVATPDWLANGLRDSIVGGVFCLLLGVCLVITEIRGIGGCSRCECNLLCFGFSVCISSLFFFTILYLSLGSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AAS4</accession>
    <name>A0AA39AAS4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein FAR1-RELATED SEQUENCE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_005451</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Putative transcription activator involved in regulating light control of development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the FHY3/FAR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9704175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AAS4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004330">
        <property type="entry name" value="FAR1_DNA_bnd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031052">
        <property type="entry name" value="FHY3/FAR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018289">
        <property type="entry name" value="MULE_transposase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006564">
        <property type="entry name" value="Znf_PMZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31669:SF293">
        <property type="entry name" value="PROTEIN FAR1-RELATED SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31669">
        <property type="entry name" value="PROTEIN FAR1-RELATED SEQUENCE 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03101">
        <property type="entry name" value="FAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10551">
        <property type="entry name" value="MULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04434">
        <property type="entry name" value="SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00575">
        <property type="entry name" value="ZnF_PMZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="8">
        <location>
            <begin position="571"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="697"/>
            <end position="719"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9704175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="735" mass="83778" checksum="FD57CB80CF108019" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AAW9</accession>
    <name>A0AA39AAW9_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_004678</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AAW9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9703019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="232" mass="25188" checksum="AEBF8AA862370548" modified="2024-01-24" version="1">MAPKRTQVVHVNKGNDKIAIAQSTHDTTTGPMTRSKAKSTSSISTKQISESACLLKQVRTRDEHQPLITLVSRGAKSQSPRSREKLPSTLQDFGDKSPCFVTDANSSTGSHFGLPVGMSKEESYSNHFDSFTSPFSVTMLVMAIGTTSVEEQLAEMAHAIAKLTKTVEEKDMQIASLINKVEAQVQNMGESSQGLNHLPNVPSPLDDAPHAYRTVQDERQMTESASVVSLSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ABD7</accession>
    <name>A0AA39ABD7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ADF-H domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_002812</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the actin-binding proteins ADF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ABD7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030042">
        <property type="term" value="P:actin filament depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11286">
        <property type="entry name" value="ADF_cofilin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000025">
        <property type="entry name" value="Actin-depolymerizing factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002108">
        <property type="entry name" value="ADF-H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017904">
        <property type="entry name" value="ADF/Cofilin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11913">
        <property type="entry name" value="COFILIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00241">
        <property type="entry name" value="Cofilin_ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00102">
        <property type="entry name" value="ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51263">
        <property type="entry name" value="ADF_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ADF-H" evidence="3">
        <location>
            <begin position="11"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9704417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="143" mass="16361" checksum="05BAB9686FAF521F" modified="2024-01-24" version="1">MAMAFKMATTGMWVTDECKNSFMEMKWKKVHRYIVFKIDEGSRLVTVDKVGGPGEGYDELAASLPTDDCRYAVFDFDFVTNDNCRKSKIFFIAWSPTASRIRAKMLYATSKEGLRRVLDGIHYDMQATDPTEMGMDVIKDRAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ABD9</accession>
    <name>A0AA39ABD9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_005258</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ABD9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47591:SF13">
        <property type="entry name" value="OS02G0293900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47591">
        <property type="entry name" value="ZINC FINGER PROTEIN ZAT2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="161"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="278"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="36"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="182"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="238"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="36"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="87"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="238"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="265"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="334"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9703902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="364" mass="38555" checksum="1C5825B5116A02AC" modified="2024-01-24" version="1">MFPALYNCYHGTALEAGDIVQNFVYPSLLHFQSFMDNNPKDSSASSPNRDRQRRSPQHANPDEHEAGGPPPTEHQGPEQHETGGTAPESSTAVSQTATEIPAATQERQSTIRVQVEVVGGDGSGQRGRGAKRSRAGEEGAGPSGTKAKKKPQEFDAPAVASPCSVCGRRFGSWKALFGHMRSHPDRDWRGIHPPPRFNREGTPEGADDYQEPDDNVVREQAENLLLGVAHEVAARLGAAPEPGKAAGEAPGGAGRDVSGSPGAKTADEGGKTETETGHRCTICSRVFSSGPALGGHMRSHSDRQDQAALSSSTQRRRGLGIDLNLPNEPEAEPPRSPSSPPPPPPEGKEGGLDLNRPPPPSDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ADB2</accession>
    <name>A0AA39ADB2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Aminotransferase class I/classII large domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_003451</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. LL-diaminopimelate aminotransferase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ADB2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009862">
        <property type="term" value="P:systemic acquired resistance, salicylic acid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000099">
        <property type="entry name" value="LL-diaminopimelate aminotransferase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01642">
        <property type="entry name" value="DapL_aminotrans_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019942">
        <property type="entry name" value="DapL/ALD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03542">
        <property type="entry name" value="DAPAT_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43144">
        <property type="entry name" value="AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="6">
        <location>
            <begin position="91"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00061511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9705407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="460" mass="50496" checksum="3CCD4B7991F67AFA" modified="2024-01-24" version="1">MYCLMSSWPSMSSSSSCFLGQTHFDSRTPNACLPTKDSWICKYVATPATETTAHTTKVSRNANIAKLQAGYLFPEIARRRSAHMLKYPEAQVISLGIGDTTEPIPEVITSGMAKKAHALSTLEGYSGYGAEQGEKQLRAAIALTYYGDLSIEESDIFVSDGAKSDISRLQVMFGSNVTMAVQDPSYPAYVDSSVILGQTGQFQKDVEKYRNIEYMKCNPENGFFPDLSTVSRTDIIFFCSPNNPTGNAATREQLTRLVQFAKDNGSILVYDSAYAMYISDDSPRSIFEIPGAKEVAIEVSSFSKYAGFTGVRLGWTVVPKELRYSDGFPVAKDFNRIVCTTFNGASNISQAGGMACLSPEGLEAMHKVVGFYKENTNRIMETFTSLGFSVYGGQNAPYVWVRFPGQSSWDVFSEILEKTHVVTTPGSGFGPAGDGFIRVSAFGHRGNVLEACKRFKQLWQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ADW6</accession>
    <name>A0AA39ADW6_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_003756</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ADW6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9705801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="187" mass="20415" checksum="18D26C23C1192C6E" modified="2024-01-24" version="1">MHVPSVASTSIASDDATEVIEARINETSETMLRLALSLVRDVGALRCWRGGSEGNSSIPCPSAEHHTEYQLMRQTPVRQPSVEEIEPQDSLAPIPHSPIGMEPSLAPTYSEPLAPQSHPEASLLPEMTIEEPREYDGASGSGRQALKPSSMKRYKRTAKRIVKRPPACKSPSVAQCVKQFPKIPHAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AGW4</accession>
    <name>A0AA39AGW4_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_002442</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AGW4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9707421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="136" mass="15663" checksum="377ADD635424FAAE" modified="2024-01-24" version="1">MGTLEIELMIGPTTFPTLFQVLRILTFFNLLLDRPWIHRAGVIPSSLHQKVKFIHDGQVITLQSMGDMFITAEPVLQISHSDDDLFLTGFTFDEVQTLEMEDFCRDFVAMSFDQHDSTMVLDIMRSMSYLPEMRLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AI45</accession>
    <name>A0AA39AI45_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Amine oxidase</fullName>
            <ecNumber evidence="20">1.4.3.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">PVL29_001911</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="25">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a primary methyl amine + O2 + H2O = an aldehyde + H2O2 + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:16153"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:228804"/>
            <dbReference type="EC" id="1.4.3.21"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:16154"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="20">
            <name>L-topaquinone</name>
            <dbReference type="ChEBI" id="CHEBI:79027"/>
        </cofactor>
        <cofactor evidence="20">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="20">Contains 1 topaquinone per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="19">
            <name>L-topaquinone</name>
            <dbReference type="ChEBI" id="CHEBI:79027"/>
        </cofactor>
        <text evidence="19">Contains 1 topaquinone per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="16">Amine and polyamine degradation; putrescine degradation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Secreted</location>
            <location evidence="4">Extracellular space</location>
            <location evidence="4">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="19 20">Topaquinone (TPQ) is generated by copper-dependent autoxidation of a specific tyrosyl residue.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 20">Belongs to the copper/topaquinone oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="25">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.-" evidence="20"/>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AI45"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008131">
        <property type="term" value="F:primary methylamine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009308">
        <property type="term" value="P:amine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.98.20:FF:000004">
        <property type="entry name" value="Amine oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.40:FF:000005">
        <property type="entry name" value="Amine oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.40:FF:000024">
        <property type="entry name" value="Amine oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.20">
        <property type="entry name" value="Copper amine oxidase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049947">
        <property type="entry name" value="Cu_Am_Ox_Cu-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049948">
        <property type="entry name" value="Cu_Am_ox_TPQ-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000269">
        <property type="entry name" value="Cu_amine_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015798">
        <property type="entry name" value="Cu_amine_oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036460">
        <property type="entry name" value="Cu_amine_oxidase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016182">
        <property type="entry name" value="Cu_amine_oxidase_N-reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015800">
        <property type="entry name" value="Cu_amine_oxidase_N2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015802">
        <property type="entry name" value="Cu_amine_oxidase_N3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10638:SF41">
        <property type="entry name" value="AMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10638">
        <property type="entry name" value="COPPER AMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01179">
        <property type="entry name" value="Cu_amine_oxid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02727">
        <property type="entry name" value="Cu_amine_oxidN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02728">
        <property type="entry name" value="Cu_amine_oxidN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49998">
        <property type="entry name" value="Amine oxidase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54416">
        <property type="entry name" value="Amine oxidase N-terminal region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01164">
        <property type="entry name" value="COPPER_AMINE_OXID_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01165">
        <property type="entry name" value="COPPER_AMINE_OXID_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0052">Apoplast</keyword>
    <keyword evidence="13 20" id="KW-0186">Copper</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 20" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 20" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0964">Secreted</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="11 18 20" id="KW-0801">TPQ</keyword>
    <feature type="domain" description="Copper amine oxidase N2-terminal" evidence="23">
        <location>
            <begin position="77"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="Copper amine oxidase N3-terminal" evidence="24">
        <location>
            <begin position="169"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="Copper amine oxidase catalytic" evidence="22">
        <location>
            <begin position="291"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="50"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="50"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="18">
        <location>
            <position position="370"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate; via topaquinone" evidence="18">
        <location>
            <position position="458"/>
        </location>
    </feature>
    <feature type="modified residue" description="2',4',5'-topaquinone" evidence="19">
        <location>
            <position position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00051381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR600269-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR600269-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU000672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF02727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF02728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="KAJ9706664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="727" mass="81583" checksum="943BA2F2448B6947" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AJU5</accession>
    <name>A0AA39AJU5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">7-dehydrocholesterol reductase</fullName>
            <ecNumber evidence="16">1.3.1.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Sterol Delta(7)-reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PVL29_000425</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ERG4/ERG24 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.21" evidence="16"/>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AJU5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047598">
        <property type="term" value="F:7-dehydrocholesterol reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016132">
        <property type="term" value="P:brassinosteroid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006695">
        <property type="term" value="P:cholesterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001171">
        <property type="entry name" value="ERG24_DHCR-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21257:SF38">
        <property type="entry name" value="7-DEHYDROCHOLESTEROL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21257">
        <property type="entry name" value="DELTA(14)-STEROL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01222">
        <property type="entry name" value="ERG4_ERG24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0152">Cholesterol biosynthesis</keyword>
    <keyword evidence="4" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0521">NADP</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="15" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="11" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="14" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="78"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="107"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00038851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00042688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ9708372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="321" mass="35957" checksum="049795A835FDE40F" modified="2024-01-24" version="1">MAEAKTVHSPALTYFSMISLPSSNYLPSLRPTVIAWKLIACYAAFEAALQLFLPGMTVEGPISPCGNRSVYKANGMQAYAVTLITYLSLWWFGILNSAIVYDHLGEMYPTLNFGSFIFCIFLYTKGMELYPRTGKNFDIKVFTNCRFGMILSEENGKVAASMIVNTVLMLVHVTKFFGWASCVPNLCKTYYPSSQPCPSNHSLTNHTSYPPIPITCSYLHLSPHHNPKVRKDAFIAFGSGLNFCNTQSSLLFPSSTPSLGQTTSALGQSTMALSVLIYLPLFFSTILVIMNFRRLLLYRVVGKTFKSFFEKFRFKSNCYQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AK95</accession>
    <name>A0AA39AK95_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Aminotransferase-like plant mobile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_000890</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AK95"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34835">
        <property type="entry name" value="OS07G0283600 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="165"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="233"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9709142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="396" mass="45466" checksum="EA3A056E76E051E1" modified="2024-01-24" version="1">MGIPHTGRKLIVDRGTVNASQMPSLQDIEAMMVDIDDIEEFKRLFLIFACATLLAPTSRLEGSHSLWYTPREQLLGNINWGEYVLEFFIQAIHEHRRKESVWIKGCLMFLQIFYFSISNFPVMYVELTTPRIAAWNDILVKQRIKLELEMLGGFGKVELVSISTEQDETEKNKAPAVDDPVDPDETEDTDVIYARMEATQRQITDAQSHLNSLIASYNRDMKVLRTRFTSSHYRTDEGQPSNFHEHVNESGARFDTNNPQHSPVHSCGEGRSFEDDVGCTNEGVSERPNVEEGVFFTNEDHLHPGPHNMLVLVQSSMENNADVTPQAPEEIRPRRVKMKARRHRQRAAACKSPFVQFCVSKYKRLTEEETHVADYVFNESKDPRLVPNHKFSKYCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AKE8</accession>
    <name>A0AA39AKE8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Aminotransferase class I/classII large domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_000706</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>glycine + 2-oxoglutarate = glyoxylate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:14089"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="EC" id="2.6.1.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>glyoxylate + L-alanine = glycine + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:24248"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="EC" id="2.6.1.44"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid degradation; L-alanine degradation via transaminase pathway; pyruvate from L-alanine: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8">Photosynthesis; C4 acid pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AKE8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008453">
        <property type="term" value="F:alanine-glyoxylate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047958">
        <property type="term" value="F:glycine:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004021">
        <property type="term" value="F:L-alanine:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009853">
        <property type="term" value="P:photorespiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1970:FF:000001">
        <property type="entry name" value="Alanine aminotransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000012">
        <property type="entry name" value="alanine aminotransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000010">
        <property type="entry name" value="Alanine aminotransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045088">
        <property type="entry name" value="ALAT1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11751">
        <property type="entry name" value="ALANINE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11751:SF373">
        <property type="entry name" value="GLUTAMATE--GLYOXYLATE AMINOTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="11">
        <location>
            <begin position="84"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00052537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9708829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="491" mass="54483" checksum="BD97DA5846A7F5C1" modified="2024-01-24" version="1">MSHKGLDYETLNENVKKVQYAVRGELYLRASELQKEGKKIIFTNVGNPHALGQKPLTFPRQVVALCQAPFLLDDPNVGLVFPADAIARAKHYLSLTTGGLGAYSDSRGLPGIRKEVAEFIERRDGYPSDPELIFLTDGASKGVMQILNTIIRGEKDGILVPVPQYPLYSAAISLFGGSLVPYYLEETANWGLDVNNLHSSVAEARSKGITVRAMVIINPGNPTGQCLSEANLKEILNFCNQENLVLLGDEVYQQNIYQDERPFISARKVLLDMGPPISKELQLVSFHTVSKGYWGECGQRGGYFEMTNIPPQTVDEIYKVASISLSPNVPGQIFLGVMVNPPKPGDISYLQFMRESKGILESLRRRAQIMTDGFNSCRNVVCNFTEGAMYSFPQIRLPPKAIEAAKKLGKVADVFYCLKLLEATGISTVPGSGFGQKEGVFHLRTTILPAEEDMPAIMESFKKFNDEFMEQYEDHRGYSRIREGETSRVSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AL69</accession>
    <name>A0AA39AL69_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Glyceraldehyde-3-phosphate dehydrogenase</fullName>
            <ecNumber evidence="10">1.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_001270</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.-" evidence="10"/>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AL69"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004365">
        <property type="term" value="F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18126">
        <property type="entry name" value="GAPDH_I_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05214">
        <property type="entry name" value="GAPDH_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000001">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000020">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020831">
        <property type="entry name" value="GlycerAld/Erythrose_P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020830">
        <property type="entry name" value="GlycerAld_3-P_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020829">
        <property type="entry name" value="GlycerAld_3-P_DH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020828">
        <property type="entry name" value="GlycerAld_3-P_DH_NAD(P)-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006424">
        <property type="entry name" value="Glyceraldehyde-3-P_DH_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01534">
        <property type="entry name" value="GAPDH-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10836">
        <property type="entry name" value="GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10836:SF109">
        <property type="entry name" value="GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02800">
        <property type="entry name" value="Gp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00044">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000149">
        <property type="entry name" value="GAP_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00078">
        <property type="entry name" value="G3PDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00846">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00071">
        <property type="entry name" value="GAPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="3 7" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyceraldehyde 3-phosphate dehydrogenase NAD(P) binding" evidence="11">
        <location>
            <begin position="8"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="158"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="17"/>
            <end position="18"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="157"/>
            <end position="159"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="217"/>
            <end position="218"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="322"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="site" description="Activates thiol group during catalysis" evidence="8">
        <location>
            <position position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9709710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="340" mass="36907" checksum="D31D345D3FB13890" modified="2024-01-24" version="1">MESSDKKIKIGINGFGRIGRLVARVALQSNDVELVAVNDPFITTDYMTYMFKYDSVHGHWKHHDVKVKDSKTLLFGEKSVTVFGVRNPEEIPWAETGADYVVESTGVFTDKDKAAAHLKGGAKKVIISAPSKDAPMFVVGVNEKEYKPHIDIVSNASCTTNCLAPLAKVINDRFGIVEGLMTTVHAITATQKTVDGPSNKDWRGGRAASFNIIPSSTGAAKAVGKVLPALNGKLTGMSFRVPTVDVSVVDLTVRLEKSATYDEVKAAIKEESEGKLKGILGYTEDDVVSTDFIGDSRSSIFDAKAGIALNANFLKLVSWYDNEWGYSSRVIDLIRHMASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D409</accession>
    <name>A0AA39D409_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">oligopeptidase A</fullName>
            <ecNumber evidence="8">3.4.24.70</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_026710</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>Hydrolysis of oligopeptides, with broad specificity. Gly or Ala commonly occur as P1 or P1' residues, but more distant residues are also important, as is shown by the fact that Z-Gly-Pro-Gly-|-Gly-Pro-Ala is cleaved, but not Z-(Gly)(5).</text>
            <dbReference type="EC" id="3.4.24.70"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9">Binds 1 zinc ion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the peptidase M3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.70" evidence="8"/>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D409"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006518">
        <property type="term" value="P:peptide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06456">
        <property type="entry name" value="M3A_DCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1370.10:FF:000017">
        <property type="entry name" value="Oligopeptidase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000009">
        <property type="entry name" value="Oligopeptidase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.40">
        <property type="entry name" value="Endopeptidase. Chain P, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1370.10">
        <property type="entry name" value="Neurolysin, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034005">
        <property type="entry name" value="M3A_DCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024077">
        <property type="entry name" value="Neurolysin/TOP_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024080">
        <property type="entry name" value="Neurolysin/TOP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045666">
        <property type="entry name" value="OpdA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045090">
        <property type="entry name" value="Pept_M3A_M3B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001567">
        <property type="entry name" value="Pept_M3A_M3B_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11804:SF83">
        <property type="entry name" value="LD37516P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11804">
        <property type="entry name" value="PROTEASE M3 THIMET OLIGOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01432">
        <property type="entry name" value="Peptidase_M3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19310">
        <property type="entry name" value="TOP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 9" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2 9" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Oligopeptidase A N-terminal" evidence="12">
        <location>
            <begin position="122"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M3A/M3B catalytic" evidence="11">
        <location>
            <begin position="324"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="240"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00026100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF19310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9670331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="792" mass="88514" checksum="023F51D189425896" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D5K0</accession>
    <name>A0AA39D5K0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">NB-ARC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_026097</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D5K0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057135">
        <property type="entry name" value="At4g27190-like_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050905">
        <property type="entry name" value="Plant_NBS-LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463:SF187">
        <property type="entry name" value="AND NB-ARC DOMAIN DISEASE RESISTANCE PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23247">
        <property type="entry name" value="LRR_RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0611">Plant defense</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="598"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="7">
        <location>
            <begin position="134"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="9">
        <location>
            <begin position="364"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein At4g27190-like leucine-rich repeats" evidence="8">
        <location>
            <begin position="668"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="21"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="780"/>
            <end position="811"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9672743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="918" mass="107511" checksum="FC2634A12F85D8CD" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D6H0</accession>
    <name>A0AA39D6H0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Mitochondrial phosphate carrier protein 1, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_027121</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D6H0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990547">
        <property type="term" value="P:mitochondrial phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000070">
        <property type="entry name" value="Mitochondrial phosphate carrier protein 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044677">
        <property type="entry name" value="SLC25A3/Pic2/Mir1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45671:SF14">
        <property type="entry name" value="PHOSPHATE CARRIER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45671">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25 (MITOCHONDRIAL CARRIER PHOSPHATE CARRIER), MEMBER 3, LIKE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="18"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="111"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="212"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9670987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="310" mass="34413" checksum="436074E3B869767F" modified="2024-01-24" version="1">MRGVEERRVCGELTPGPGYFWVCTIGGMLSAGTTHVAITPLDVLKVNMQVYPIKYNTISSCFSTLLREQGPSAFWRGWATKFFGYGVQGGFRFGLYEYFKKLYSDVLVDHNRSFIFFASSASAEVLANVALCPFEAVKVRVQAQPHFAKGLLDGFPKLYASEGLHGFYRGLVPLWGRNLPFSMIMFSTFEHSVDFLYRNVIHRRKEDCSRVQQLGVTCLAGCAAGSVASLITNPADNIVASLYNRKADSLLLAVKKIGLMNLFTRSLPIRIILVGPVVTLQWLVYDTIKVLSGLPTSGEVLTDKEVNKKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D7B9</accession>
    <name>A0AA39D7B9_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_023128</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D7B9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048364">
        <property type="term" value="P:root development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048367">
        <property type="term" value="P:shoot system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004320">
        <property type="entry name" value="BPS1_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33070">
        <property type="entry name" value="OS06G0725500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33070:SF49">
        <property type="entry name" value="OS06G0725500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03087">
        <property type="entry name" value="BPS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9673359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="292" mass="33350" checksum="4CBE349543D7B16A" modified="2024-01-24" version="1">MVAAVFRRTLSFPNSSLSRPSKPAKTYHVRSISLPCRPHPLISQLKEEIKELRAWESKLGERTSAWLCEGLSRLRTVHYSLDDILQLPQTQELLRRQPAAVEKLLEDFLGFVDLYGLFQTSVLALKEEQSAAQVAIRRRDESKVALFVKAQKRMNKEMGKLISTVRSTGRFPNGDGELISIIRDVNQVTVLVSVALFNGVSSSWASRKFPWKGLRLSKKAKRVKVEECIQEFQQVGINIFWGLKKKGNEEVRIDLKRMQALENCIRGIELCSERVFRSLINTRVSLLNILTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D7Q7</accession>
    <name>A0AA39D7Q7_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_025774</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D7Q7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="13"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9672292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="61" mass="6892" checksum="A7EBEC022D2F850B" modified="2024-01-24" version="1">MVTCYPSTPLKKALHVGCFLFGATLMCIGGYLSFANVERQQALIKARNDFVRERLKRRSGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D8A0</accession>
    <name>A0AA39D8A0_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_023738</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D8A0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000008">
        <property type="entry name" value="Tryptamine hydroxycinnamoyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051283">
        <property type="entry name" value="Sec_Metabolite_Acyltrans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31896">
        <property type="entry name" value="FAMILY REGULATORY PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G14730)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31896:SF12">
        <property type="entry name" value="HXXXD-TYPE ACYL-TRANSFERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9674364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="454" mass="50554" checksum="7F90B0E3DB506DDF" modified="2024-01-24" version="1">MATPAVRHISECFIKPKYKSEESKEPYYLTPWDLAMLSLNYIQKGLLFTKPSAVNDPQNFMTTLLDRLKDSLALILVHFYPLAGRLVTKREENPPSYLVFVDCNNSPGAKFIHTAADITISDILSPIHVPSVVQSFFDHDRVVNHDAHTLSLSLLSIQVTELLDGIFIGCSANHSIVDGTSFWHFLNAWSEIFNLQEKNTSISRPPILKRWFPDGCGPVVSLPFTHHDRFNSRIEAPILRERIFHFSSESIAKLKAKANAECNSNKISSFQALSALVWRSITRAHGLPHEQKTICGWAINNRTRLDPPLPENNFGNSIQIVSGIATAGELLEHNLGWAAWLLHKAVVSHTDKAVRGWLESWLQSPVVYQLGLFFDPNNVIIGSSPRFNKYGNEFGLGKALALRSGNAHKFDGMVSCYPGREGGGSIDLEVCLTLNSMSALESNPEFMEAVSISS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D9U8</accession>
    <name>A0AA39D9U8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">mannan endo-1,4-beta-mannosidase</fullName>
            <ecNumber evidence="4">3.2.1.78</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_025039</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random hydrolysis of (1-&gt;4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.</text>
            <dbReference type="EC" id="3.2.1.78"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.78" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9676316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D9U8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016985">
        <property type="term" value="F:mannan endo-1,4-beta-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000012">
        <property type="entry name" value="Mannan endo-1,4-beta-mannosidase 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045053">
        <property type="entry name" value="MAN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31451">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31451:SF53">
        <property type="entry name" value="MANNAN ENDO-1,4-BETA-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26410">
        <property type="entry name" value="GH5_mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041453989" description="mannan endo-1,4-beta-mannosidase" evidence="8">
        <location>
            <begin position="24"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="9">
        <location>
            <begin position="31"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF26410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9676316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="402" mass="45366" checksum="96F163A05BBA0AAA" modified="2024-01-24" version="1">MAYYHRSSLLSVLFLLVALVCEARVLQSPGFVQTQNTQFVLDGSPFFFNGFNSYWMMNVAADPSQKNKISEVFGQATASRLSVCRTWAFNDGGNQALQISPGVYDERVFQGLDFVISEAKRYGVRLILSLSNNYKDFGGRPQYVNWAKSAGAPVNKDDDFYTSEVVKGYYKNHVKRVLTRINTITRVAYKDDPTIMAWELINEPRCQVDYSGKTLNGWIQEMATYVKSIDNKHLLTVGMEGFYGDSMPEKKAINPGYQVGTDFISNHLIKEIDFTTIHAYPDIWLSGKDDSSQMAFMQRWTMSHWTDSRTIIKKPMVFSEFGKSSKDPGYSLSARDSFLNAVYTNIYNFARNGGIGGGLVWQLMAEGMQSYDDGYEIVLSQTPSTSGLITQQSNKMIALDRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DAQ4</accession>
    <name>A0AA39DAQ4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_022874</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Probable component of the PAM complex at least composed of a mitochondrial HSP70 protein, TIMM44 and TIMM14. The complex interacts with the TIMM23 component of the TIM17:23 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9678113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DAQ4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001405">
        <property type="term" value="C:PAM complex, Tim23 associated import motor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000001">
        <property type="entry name" value="Import inner membrane translocase subunit tim14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12763">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12763:SF42">
        <property type="entry name" value="OS03G0776900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="7">
        <location>
            <begin position="81"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00059031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00063640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9678113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="140" mass="15600" checksum="1EA9F7F4183EB59B" modified="2024-01-24" version="1">MKKLFILNLGVAMFGDNWCWNSELCHFAMESPLVLGVTVATAALGGRYMIRAWQALKARPSVPRIRRFYEGGFQHSMTRREAALILGVREHAVMEKIKEAHRRVMVANHPDSGGSHYLASKINEAKDVLMGRAKGTGSAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DD64</accession>
    <name>A0AA39DD64_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PVL29_021314</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DD64"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000590">
        <property type="entry name" value="PR5-like receptor kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000059">
        <property type="entry name" value="S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045874">
        <property type="entry name" value="LRK10/LRL21-25-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025287">
        <property type="entry name" value="WAK_GUB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27009">
        <property type="entry name" value="RUST RESISTANCE KINASE LR10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13947">
        <property type="entry name" value="GUB_WAK_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7 19" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041286614" description="non-specific serine/threonine protein kinase" evidence="19">
        <location>
            <begin position="25"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
            <begin position="282"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="310"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAJ9679345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="587" mass="66521" checksum="24D50AD4154D6E02" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DE47</accession>
    <name>A0AA39DE47_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Trans-resveratrol di-O-methyltransferase-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_019644</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DE47"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009717">
        <property type="term" value="P:isoflavonoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000213">
        <property type="entry name" value="Coniferyl alcohol 9-O-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000057">
        <property type="entry name" value="O-methyltransferase ZRP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016461">
        <property type="entry name" value="COMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001077">
        <property type="entry name" value="COMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012967">
        <property type="entry name" value="COMT_dimerisation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11746">
        <property type="entry name" value="O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08100">
        <property type="entry name" value="Dimerisation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00891">
        <property type="entry name" value="Methyltransf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005739">
        <property type="entry name" value="O-mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51683">
        <property type="entry name" value="SAM_OMT_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="O-methyltransferase dimerisation" evidence="6">
        <location>
            <begin position="21"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="O-methyltransferase C-terminal" evidence="5">
        <location>
            <begin position="131"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR005739-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9680380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="348" mass="38796" checksum="1C330B63A450D89F" modified="2024-01-24" version="1">MDLGSAERADELLQAQAHVVNHAFNFMNSMALKCAIDLAIPDVIHSHGQPMLLSQLVTALSVQPSKVPCLHRLMRLLVHSGFFSQQQVIHNNEQEEGYSLTSASRFLLKDEPLTGLPLSLLHLNPVLTAPWHFLSGWFRNGDPTPFYTAHGKPYWDYTAQEPDLNDLFNEAMASDSRIIQFKGLSSVVDVGAGTGTMTKAIAKAFPHLKCIVFDQPHVVAHLQGGGNLEVVGGDMFEAIPSANAVILKWILHNWSDEECVKILKKCKEAIPSKDKAGKLMIIDMVMKNNKGDDQAVETQLFWDMLMMTVLTGKQRNENEWKKLFVTAGFTHYKISAVLGFRSLIEVYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DE62</accession>
    <name>A0AA39DE62_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_020982</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DE62"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000111">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001347">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000299">
        <property type="entry name" value="Piriformospora indica-insensitive protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001488">
        <property type="entry name" value="Receptor-like protein EIX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000213">
        <property type="entry name" value="Tyrosine-sulfated glycopeptide receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046956">
        <property type="entry name" value="RLP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063">
        <property type="entry name" value="LRR RECEPTOR-LIKE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063:SF101">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE FLS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041364303" description="Leucine-rich repeat-containing N-terminal plant-type domain-containing protein" evidence="13">
        <location>
            <begin position="26"/>
            <end position="1061"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1035"/>
            <end position="1058"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="14">
        <location>
            <begin position="33"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="15">
        <location>
            <begin position="341"/>
            <end position="544"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9678937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1061" mass="117372" checksum="2DADCFC02CF483DF" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DEV0</accession>
    <name>A0AA39DEV0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_018304</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DEV0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35218:SF9">
        <property type="entry name" value="ENDONUCLEASE_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35218">
        <property type="entry name" value="RNASE H DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="1">
        <location>
            <begin position="6"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9682358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="151" mass="17159" checksum="4D875203A2E01D47" modified="2024-01-24" version="1">MKIRILSWNIRGANNCDKIKVIKALIKKNRVDLGCLQETKIQEMSRGLIRSLGVGRFLKWGAVDSRGSTGGIVVFWDNRDGFSWTFTGVYGSTIRRDRECLWNELEAIYGLWNGLWCVAGDFNAILSPEEHSRGGSLNSDMRRFSDVIEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DH09</accession>
    <name>A0AA39DH09_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_016332</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9683786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DH09"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9683786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="209" mass="23407" checksum="B4379B55316F579F" modified="2024-01-24" version="1">MFAPSHSIIHNLPHSWSSLHHPLNLHPSIPIFTTVKPIFPANPLHVRQFDNQAPPANHKGTDPEPCKRQTVEDFEHERPIWKELLSLICSKTLSKTSLMVLMGTDAVMGCSVKLHVERMGDKILLCRVHSLVGNIWLIFTKGDLKGVSDQISIEDHMSRWIPGNLNRDHLGKHQSGPEKLSLPIVPCASVSLQSLNETVKLVKQRGAWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DJZ4</accession>
    <name>A0AA39DJZ4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">peroxidase</fullName>
            <ecNumber evidence="3">1.11.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_015822</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:56136"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:139520"/>
            <dbReference type="ChEBI" id="CHEBI:139521"/>
            <dbReference type="EC" id="1.11.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="9">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the peroxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.7" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DJZ4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140825">
        <property type="term" value="F:lactoperoxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000823">
        <property type="entry name" value="Peroxidase_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31235">
        <property type="entry name" value="PEROXIDASE 25-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50873">
        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0349">Heme</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="6 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Plant heme peroxidase family profile" evidence="12">
        <location>
            <begin position="43"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="20"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9687116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="105" mass="11413" checksum="48FD211B4C6786B3" modified="2024-01-24" version="1">MAAALAQGQGTRVGFYSYTCPEVESIVKDCYCSFQFQPNHCFDASILITGSSIERTAGPNSLFRGYEVIDDAQTRLEATCPGVVSCADIHSLAARDYVLLVCIYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DLW9</accession>
    <name>A0AA39DLW9_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_012358</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9689618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DLW9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9689618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="311" mass="34577" checksum="21A40F7B5D907724" modified="2024-01-24" version="1">MNPITSLPIIQRHNLFHCQEGNEQCEKHNHPLRSHILHPNKHMRHKSEQQAHNRVQKPNRKTLSPKLHTRPLIKEYKFGGIVPIGQKHGHFPNYIMHGARNTEEIDKHSTPQPNLRIMKQCLAGQNLLHNKRRPHQEGRHKESQLLLGEHRRPLDWADRELGPGLDEVGHRVGHARNVPLLHLLRILEALHIGANKSDVGERVDPKLGETGLGLFELDVAEEELGGGGRVLVVGDEGGGEEAELGAKALEVEFGLVMELSGVEEDEDRVGPRGCGGGEEAAEGVIVEVDHARFGSRLEREKLSNPVGFGAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DMC7</accession>
    <name>A0AA39DMC7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Chromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_014731</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9689214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DMC7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005162">
        <property type="entry name" value="Retrotrans_gag_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03732">
        <property type="entry name" value="Retrotrans_gag"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chromo" evidence="2">
        <location>
            <begin position="483"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="318"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="456"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="318"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="353"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="460"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9689214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="546" mass="62437" checksum="6344FE8095020AB6" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DMT6</accession>
    <name>A0AA39DMT6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Prolamin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_014784</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9689275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DMT6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031982">
        <property type="term" value="C:vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009567">
        <property type="term" value="P:double fertilization forming a zygote and endosperm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080155">
        <property type="term" value="P:regulation of double fertilization forming a zygote and endosperm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000008">
        <property type="term" value="P:regulation of protein localization to cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008502">
        <property type="entry name" value="Prolamin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31181">
        <property type="entry name" value="EGG CELL-SECRETED PROTEIN 1.4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31181:SF67">
        <property type="entry name" value="PROLAMIN-LIKE PROTEIN (DUF1278)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05617">
        <property type="entry name" value="Prolamin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Prolamin-like" evidence="2">
        <location>
            <begin position="45"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9689275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="122" mass="13622" checksum="3931702342E6EB75" modified="2024-01-24" version="1">MAEKSTKVLAVLLMVSCMVAYVPTGLALRPIEHKLFHLPTGFFSDCWKAFTGLNGCVTQFYAAIITGGYDRIRPTCCQAINSIVNRCWHTFFHNDPSIPPSLKRYCVQSTTVAKPLSQDNLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DQ76</accession>
    <name>A0AA39DQ76_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_011937</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9693023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DQ76"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010330">
        <property type="term" value="C:cellulose synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051211">
        <property type="term" value="P:anisotropic cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001006">
        <property type="term" value="P:regulation of cellulose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044297">
        <property type="entry name" value="CSI1/2/3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46369">
        <property type="entry name" value="PROTEIN CELLULOSE SYNTHASE INTERACTIVE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46369:SF1">
        <property type="entry name" value="PROTEIN CELLULOSE SYNTHASE INTERACTIVE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9693023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="113" mass="12689" checksum="303208DAF11BEC3F" modified="2024-01-24" version="1">MDLDETDAVDALQVVSLLAKMKQSMNFTYSPWSALAEIPSSLESLVRCLAEFAFSGAIVLDDLLVEQSRSIGSLTNRKMNSSSLGELVELSYSLVLQRNVNRLQWMHLMYQDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DQG9</accession>
    <name>A0AA39DQG9_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_012906</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DQG9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.700">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47372">
        <property type="entry name" value="DAUER UP-REGULATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47372:SF24">
        <property type="entry name" value="LATE EMBRYOGENESIS ABUNDANT PROTEIN (LEA) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="156"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="175"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="234"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9690487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="270" mass="29422" checksum="57E20D3F04284D11" modified="2024-01-24" version="1">MAAMFIAQNTMLNLSRAFPRNRSIALRSPRKVSRVCFTSASKSSEGRNAAEEARENGSNREGRASEMTERAKEKTRESMDRTKEQAEGVKDRAKQRAEDMAEKGKEGTYKATETAEEAKEKAKERAREMKEKTSDMAESAAERAKEGTYKATETAEQAKESAKESAREAKEKTSDMAASAADKAKQGASKATETVGAVVDKAKEGGSKAAETAQNLGEKAKQTVQGAWGAAKETTQKIKETVVGKSEDKGKSAEDYVEDLRMPEDKDKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DR89</accession>
    <name>A0AA39DR89_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myosin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_012085</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9693189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DR89"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="1" id="KW-0505">Motor protein</keyword>
    <keyword evidence="1" id="KW-0518">Myosin</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myosin motor" evidence="2">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9693189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="62" mass="7180" checksum="528E6F1CB115D9E6" modified="2024-01-24" version="1">MDQKVKPNRDLMAPLSLRKELDLKSAYEYKYWKQSNCYSITGVDDAEQLHAVVEALDIVHVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DRJ3</accession>
    <name>A0AA39DRJ3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 19">Peroxidase</fullName>
            <ecNumber evidence="4 19">1.11.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PVL29_013719</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 19">
            <text>2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:56136"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:139520"/>
            <dbReference type="ChEBI" id="CHEBI:139521"/>
            <dbReference type="EC" id="1.11.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16 19">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="16 19">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16 19">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="16 19">Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="19">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peroxidase family. Ascorbate peroxidase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.7" evidence="4 19"/>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DRJ3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140825">
        <property type="term" value="F:lactoperoxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00693">
        <property type="entry name" value="secretory_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.420.10:FF:000001">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.520.10:FF:000008">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.420.10">
        <property type="entry name" value="Peroxidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000823">
        <property type="entry name" value="Peroxidase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019794">
        <property type="entry name" value="Peroxidases_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019793">
        <property type="entry name" value="Peroxidases_heam-ligand_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033905">
        <property type="entry name" value="Secretory_peroxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31517">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31517:SF59">
        <property type="entry name" value="PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00458">
        <property type="entry name" value="PEROXIDASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00461">
        <property type="entry name" value="PLPEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00435">
        <property type="entry name" value="PEROXIDASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00436">
        <property type="entry name" value="PEROXIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50873">
        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 16 19" id="KW-0106">Calcium</keyword>
    <keyword evidence="12 18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 19" id="KW-0349">Heme</keyword>
    <keyword evidence="13 19" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="11 16 19" id="KW-0408">Iron</keyword>
    <keyword evidence="8 16 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 19" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6 19" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 19" id="KW-0964">Secreted</keyword>
    <keyword evidence="19" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041490386" description="Peroxidase" evidence="19">
        <location>
            <begin position="28"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="Plant heme peroxidase family profile" evidence="20">
        <location>
            <begin position="33"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="74"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="16">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="17">
        <location>
            <position position="70"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="43"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="76"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="130"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="209"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU362060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAJ9691612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="336" mass="37266" checksum="75335813B0141AF5" modified="2024-01-24" version="1">MAKVSFSLLFAFYFLFLQWVLVPYTATAYNASGLSYDFYEKSCPNVERIIHNVVSQKFTEAFSTAGGALRIFFHDCFVEGCDASVLIASSKTNKAERDAEINLSLPGDGYEVFFRAKRALELQCPGVVSCTDVMAIATRDLLNLVGAPRWEVLKGRRDGLVSKASRVAGNIPEPTQTVSELISLFKSKGLSVLDMVALSGGHTIGFSHCDQFMSRIYSFNKTFDIDPTMDKDYAQMLQESCPEKTFDRNIVLPNDVSTPQAFDNAYYNNLQKGLGLLSSDQILALDPTTQGYVNSMAENQQVFFRHFVRAMIKLGEIGVKTGSNGEIRRDCGVFNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DRV3</accession>
    <name>A0AA39DRV3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Disease resistance R13L4/SHOC-2-like LRR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_012238</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9693385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DRV3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000383">
        <property type="entry name" value="Leucine-rich repeat receptor protein kinase EMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000041">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001347">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000213">
        <property type="entry name" value="Tyrosine-sulfated glycopeptide receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046956">
        <property type="entry name" value="RLP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063">
        <property type="entry name" value="LRR RECEPTOR-LIKE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063:SF16">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="787"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="13">
        <location>
            <begin position="9"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9693385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="842" mass="94575" checksum="12F6D2993447072D" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DSD8</accession>
    <name>A0AA39DSD8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_009738</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9693910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DSD8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050872">
        <property type="entry name" value="PPR_P_subfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46128">
        <property type="entry name" value="MITOCHONDRIAL GROUP I INTRON SPLICING FACTOR CCM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46128:SF145">
        <property type="entry name" value="PENTACOTRIPEPTIDE-REPEAT REGION OF PRORP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12854">
        <property type="entry name" value="PPR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="15"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="140"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="196"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="231"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="266"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="301"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="336"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="371"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="406"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="476"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="511"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="581"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="616"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="652"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="756"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="791"/>
            <end position="825"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9693910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="842" mass="94713" checksum="79CEF5FC2431DDDB" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DUE3</accession>
    <name>A0AA39DUE3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glutathione transferase</fullName>
            <ecNumber evidence="3">2.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_008605</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GST superfamily. Phi family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DUE3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043295">
        <property type="term" value="F:glutathione binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009407">
        <property type="term" value="P:toxin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03187">
        <property type="entry name" value="GST_C_Phi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000004">
        <property type="entry name" value="Glutathione S-transferase F2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000016">
        <property type="entry name" value="Glutathione S-transferase F2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034347">
        <property type="entry name" value="GST_Phi_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43900">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43900:SF37">
        <property type="entry name" value="GLUTATHIONE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0216">Detoxification</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="8">
        <location>
            <begin position="2"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="9">
        <location>
            <begin position="88"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9696459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="215" mass="24628" checksum="CA82843A79F50411" modified="2024-01-24" version="1">MAVRKLYGALDCPSTIRALASLLEHDVEFELIPVDLQAGELKKMSSLSPFGELPVFQEGDLTIFESRTIMRYISHEYGKRGEEQVYEIPKLQGIAAAWIDVEDHQFDPPASKLIWELVFKPQKGLPTDEGVVEEEEAKLVKVLDVYEERLSNSVFLGGDKFTSADLTHIPSLYLLMKTPVKRLFEERPRVRAWYRSVTSRPGWEKMVEMVEKVRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DUG7</accession>
    <name>A0AA39DUG7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_007231</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DUG7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.80">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003441">
        <property type="entry name" value="NAC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036093">
        <property type="entry name" value="NAC_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31719">
        <property type="entry name" value="NAC TRANSCRIPTION FACTOR 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31719:SF179">
        <property type="entry name" value="OS08G0148400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02365">
        <property type="entry name" value="NAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101941">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51005">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NAC" evidence="6">
        <location>
            <begin position="41"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="197"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="197"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9698021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="401" mass="45263" checksum="E4AEEF5C55F8A708" modified="2024-01-24" version="1">MVGGTQVPVGGSRIPKPEEAGEEIRILDYRFKNKDEFLNSMPPGYKFSPTDEEVILYYLRPKIENKPLPINRIVEVDLYQYDPEDLTALFPPCGNYNDEIAAWFFFTPRDRKYPNGMRPSRRAGKGYWKAIAGDKAIKCEDQTIGYRKVLVFHRGKAPKGEKTNWLMYEFRGKEPPRSPSANTMSLDIVLSKIYKKPNKPLTRRSSTHAPEPEPEPPHPVKVEAGGMEQNADQTRLFTQGTGQELNQIISYVDPYEEQCYYNGSQMPLQTQQPQPTTEIFQTPQEHDNFALQSNTQFPILDPSVFMPLPTLHQPIHCIGNGFGGQGLDNFDIMNGAIGNSSFDAGNNIDFGNVPGFDSIDYGNPGICPMINYGNDAAPMSSNMNGNYDRRLLHGRNNNRQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DXF9</accession>
    <name>A0AA39DXF9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Calcium-transporting ATPase</fullName>
            <ecNumber evidence="19">7.2.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PVL29_006003</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">Catalyzes the hydrolysis of ATP coupled with the transport of calcium.</text>
    </comment>
    <comment type="function">
        <text evidence="18">This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol out of the cell or into organelles.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17 19">
            <text>Ca(2+)(in) + ATP + H2O = Ca(2+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18105"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 19">Membrane</location>
            <topology evidence="1 19">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 19">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.10" evidence="19"/>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DXF9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005388">
        <property type="term" value="F:P-type calcium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000039">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000006">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000013">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000018">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006408">
        <property type="entry name" value="P-type_ATPase_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01517">
        <property type="entry name" value="ATPase-IIB_Ca"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093:SF434">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE 13, PLASMA MEMBRANE-TYPE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093">
        <property type="entry name" value="CATION TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 19" id="KW-0106">Calcium</keyword>
    <keyword evidence="4 19" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="11" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="15 19" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="16 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 19" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="132"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="169"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="327"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="377"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="800"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="937"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="968"/>
            <end position="987"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="20">
        <location>
            <begin position="86"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00053300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU361146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAJ9700478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1003" mass="109845" checksum="9ADB43CF952FD694" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DXG3</accession>
    <name>A0AA39DXG3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF4220 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_006769</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DXG3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007658">
        <property type="entry name" value="DUF594"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31325">
        <property type="entry name" value="OS01G0798800 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04578">
        <property type="entry name" value="DUF594"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9701546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="200" mass="23124" checksum="28EBDA9EC5F7F4F2" modified="2024-01-24" version="1">MKCFCRLGWSIGEEFDQSILIWHLATDLCYYTDLNKNSSPVKNSDRKASKLLSDYMVYLLVKRPVMLPDGIGKIRFQDSCAEATEIFQCVKDRIQACKKLLLQVNTEIPSLKVKGDRSKSMLFDACKLAKSLQSLESEKEWNCEKKWEMMSHVWVEMLCYAACHCPHNRHAEELAKGGELLTHVWLLMAHFGITEHVYQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DY87</accession>
    <name>A0AA39DY87_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_005627</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9699850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DY87"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33726:SF17">
        <property type="entry name" value="OS06G0620700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33726">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9699850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="82" mass="9759" checksum="05E98A8BEE905357" modified="2024-01-24" version="1">MCGRWSASTFRWPEFDFSISFSIFRWPEFDFSYFTAGWSTQSFSWLDFSILDSVLWTFVYVLESLALVTMLCFFFVFCGCTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E085</accession>
    <name>A0AA39E085_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_005945</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E085"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46067">
        <property type="entry name" value="ACYL-COA N-ACYLTRANSFERASES (NAT) SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46067:SF18">
        <property type="entry name" value="ACYL-COA N-ACYLTRANSFERASES (NAT) SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13302">
        <property type="entry name" value="Acetyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="6"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9700387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="172" mass="19949" checksum="5432E648F2FFCD35" modified="2024-01-24" version="1">MESSRISLRPFKLSDVEDFMSWAGDDRVTRYLRWNTITSREEAFKYLKEVAIPHPWRRSICLDDRSIGYISVKPESGDDRCRAHIGYALGWEYWGQGIVTVALKMALSIVFKEFQDLVRVQALVEVENKGSQRVLEKVGFLKEGLLRKYGFNKGEVRDMFIYGFLSTDPIPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E1F2</accession>
    <name>A0AA39E1F2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Late embryogenesis abundant protein LEA-2 subgroup domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_001558</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E1F2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055301">
        <property type="entry name" value="Lea14-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004864">
        <property type="entry name" value="LEA_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31852">
        <property type="entry name" value="LATE EMBRYOGENESIS ABUNDANT (LEA) HYDROXYPROLINE-RICH GLYCOPROTEIN FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03168">
        <property type="entry name" value="LEA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117070">
        <property type="entry name" value="LEA14-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="35"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="Late embryogenesis abundant protein LEA-2 subgroup" evidence="3">
        <location>
            <begin position="92"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9706046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="209" mass="23290" checksum="A61EF65F8E53AFBB" modified="2024-01-24" version="1">MAEKDQAKPLAPAGLRSDEEEAMSRELKKLHPPKYIKCCGCITALILILAVTLLVLAFTIFRVKDPVIKMNGIRFGQLELEDGANLTLVADVSVKNPNVASFRFSNATTSITYNGTMIGQARTPPGEAKARRTLRMNVTVEIIPEKIMAVPRLLSSRAINISSYTRIAGRVKILKIIKKNVVVKLNCTMMVNLTSREIERQSCKRHLWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E1X9</accession>
    <name>A0AA39E1X9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PRISE-like Rossmann-fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_003310</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E1X9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd08948">
        <property type="entry name" value="5beta-POR_like_SDR_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000808">
        <property type="entry name" value="Iridoid synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055222">
        <property type="entry name" value="PRISE-like_Rossmann-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32487">
        <property type="entry name" value="3-OXO-DELTA(4,5)-STEROID 5-BETA-REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32487:SF0">
        <property type="entry name" value="3-OXO-DELTA(4,5)-STEROID 5-BETA-REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22917">
        <property type="entry name" value="PRISE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRISE-like Rossmann-fold" evidence="3">
        <location>
            <begin position="106"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9705188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="410" mass="46600" checksum="5E1C4BEDC0F8A6AE" modified="2024-01-24" version="1">MEENWLLFPHPICVVELKTKSIQNPPNLSCFLAQRKLQEDEAHPTNYQGVGLIVGVTGIVGNSLAEILPLRDTPGGPWKVYGVARRPQPAWNADNCVEYIQCDVFDLEETSSKLSKLTDVTHIFYVTWANMGSEAENCRVNGDMFRNVLSAVIPNAPNLQHICLQTGRKHYIGPFEAFGKIEPHDPPYHEEMPRLDVENFYHVQEDILFEEVRKKEGLTWSVHRPGIIFGFSPYSMMNAIGTLCVYATICKHEGLPLRFPGTQDTWNGYWDASDADLIAEHHIWAAVDPFAKNEAFNCSNGDVFKWKHLWKVLAEQFGLEFHEPEGQGLSLEKMMKDKGPVWDEIVREKGLVPTKLEEVGQWWYADVILSAGSTLDSMNKSKEHGFLGFRNSKSSFLSWIDKMKGYKLVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E2U7</accession>
    <name>A0AA39E2U7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">60S ribosomal export protein NMD3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_004712</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Acts as an adapter for the XPO1/CRM1-mediated export of the 60S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the NMD3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E2U7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039768">
        <property type="entry name" value="Nmd3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007064">
        <property type="entry name" value="Nmd3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048899">
        <property type="entry name" value="NMD_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048898">
        <property type="entry name" value="OB_NMD3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12746:SF2">
        <property type="entry name" value="60S RIBOSOMAL EXPORT PROTEIN NMD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12746">
        <property type="entry name" value="NONSENSE-MEDIATED MRNA DECAY PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04981">
        <property type="entry name" value="NMD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21193">
        <property type="entry name" value="NMD_SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21192">
        <property type="entry name" value="OB_NMD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Nmd3 N-terminal" evidence="8">
        <location>
            <begin position="19"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="60S ribosomal export protein NMD3 SH3" evidence="10">
        <location>
            <begin position="251"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="60S ribosomal export protein NMD3 OB-fold" evidence="9">
        <location>
            <begin position="315"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF21192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF21193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9703057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="510" mass="58519" checksum="5DEDFE86DF42857F" modified="2024-01-24" version="1">MAEEAGMFVVHQTVGSVLCCKCGIPMAPNAANMCVKCLRSEIDITEGLQKNVTIVHCPECDTYLQPPKTWIKAQLESKELLTFCVKRLKNLNRVRLIHAEFIWTEPHSKRIKVKLRVQKEVLNGAILEQAYIVEYVQQEHMCESCSRVQANPDQWVASVQLRQHVSHRRTFFYLEQLILRHDAAGRAIKIKQMDQGIDFFFANRSHGVKFVEFIGKVTPIRSRHDKQLVSHDTKSNNYNYKYTFSVEICPICREDLICLPPKVAVNLGNLGPLVICTKVSNSIALLDPFTLRHCFMDADQYWRSSFKALLSSRQLVEYIVLDVEIVSSEVNVGGSKYALADAQVARVSDFGKNDTIFNIRTHLGHLLNPGDHALGYDLYAANSNDMELEKYRGFVLPEAILIKKSYEEKRQRKRGKPRSWKLKSLNMEVDDSKGRADQEKMNTEYEVFLRDLEENPELRFNISLYRNKEYQPSEMASMTDGEDLPSVPLEELLADLDLSEEEDGDDSMRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E310</accession>
    <name>A0AA39E310_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NmrA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_003018</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NmrA-type oxidoreductase family. Isoflavone reductase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E310"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05259">
        <property type="entry name" value="PCBER_SDR_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008030">
        <property type="entry name" value="NmrA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050608">
        <property type="entry name" value="NmrA-type/Isoflavone_red_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045312">
        <property type="entry name" value="PCBER-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43349:SF35">
        <property type="entry name" value="PHENYLCOUMARAN BENZYLIC ETHER REDUCTASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43349">
        <property type="entry name" value="PINORESINOL REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05368">
        <property type="entry name" value="NmrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NmrA-like" evidence="4">
        <location>
            <begin position="3"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9704770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="309" mass="33931" checksum="EBE99171551A26E3" modified="2024-01-24" version="1">MSEKSKILIIGGTGYIGKFIVAASARSGHPTFALVRESNVSNPSKSEIIESFKSSGVTLVYGDLHDHESLVKAIKQVDVVISTVGRAQFSDQVKIIAAIKEAGNVKRFFPSEFGNDVDRIHAVGPAKIAEGIPYTYVSSNFFAGFFLPRLSQLEATAAFFVSRLSQPGATGPPRDKIVIPGDGNPKAVFNKEDDIGTYTIKAVDDPRTLNKNLYIRPPQNTYSYNEIVSLWEKKIGKTLEKIYVPEEQVLKNIQEASAPLNAILSIDHSVFIKGDQTNFEIEPSFGVEASELYPDVKYTTVDELLNQLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E4C8</accession>
    <name>A0AA39E4C8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Thaumatin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_001878</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thaumatin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E4C8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.110.10:FF:000003">
        <property type="entry name" value="Thaumatin I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.110.10">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037176">
        <property type="entry name" value="Osmotin/thaumatin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001938">
        <property type="entry name" value="Thaumatin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017949">
        <property type="entry name" value="Thaumatin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31048">
        <property type="entry name" value="OS03G0233200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00314">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002703">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00347">
        <property type="entry name" value="THAUMATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00205">
        <property type="entry name" value="THN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49870">
        <property type="entry name" value="Osmotin, thaumatin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00316">
        <property type="entry name" value="THAUMATIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51367">
        <property type="entry name" value="THAUMATIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041206270" description="Thaumatin-like protein" evidence="4">
        <location>
            <begin position="25"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="33"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="74"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="89"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="146"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="154"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="168"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="178"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR002703-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9706629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="225" mass="24110" checksum="58493B80D36098DB" modified="2024-01-24" version="1">MGLFKSLSISFFLLATLFFTSAHAAIFNIQNHCPYTVWAAAVPGGGRRLDPSQTWSLTVNPGTTGARIWARTGCSFDGAGRGQCQTGDCGGVLQCTAYGAAPNTLAEFALNQFNNLDFFDISLVDGFNVPLAFNPTSNGCTRGIQCTADIVGQCPSQLRAQGGCNNPCTVFKTDQYCCNSGSCGPTDYSRFFKDRCPDAYSYPKDDQTSTFTCPGGTDYDVNFCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YH51</accession>
    <name>A0AA38YH51_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">tRNA-dihydrouridine synthase</fullName>
            <ecNumber evidence="6">1.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_026722</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 6 8">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the dus family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.-" evidence="6"/>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YH51"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017150">
        <property type="term" value="F:tRNA dihydrouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02801">
        <property type="entry name" value="DUS_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035587">
        <property type="entry name" value="DUS-like_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001269">
        <property type="entry name" value="DUS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052582">
        <property type="entry name" value="tRNA-DUS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018517">
        <property type="entry name" value="tRNA_hU_synthase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45936">
        <property type="entry name" value="TRNA-DIHYDROURIDINE(20) SYNTHASE [NAD(P)+]-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45936:SF1">
        <property type="entry name" value="TRNA-DIHYDROURIDINE(20) SYNTHASE [NAD(P)+]-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01207">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006621">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01136">
        <property type="entry name" value="UPF0034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3 6 8" id="KW-0288">FMN</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="DUS-like FMN-binding" evidence="9">
        <location>
            <begin position="9"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="110"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="11"/>
            <end position="13"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="232"/>
            <end position="233"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR006621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR006621-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR006621-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9670354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="326" mass="36350" checksum="DFF23F519696F2EE" modified="2024-01-24" version="1">MEYGNRLVLAPMVRVGTLPFRLLAAQYGADITYGEEIIDHKLIKCERRLNEDIGSIDFVEKGTHNVVFRTCHEEKNRVVFQMGTSDAQRALTAAQIVCKDVAAVDINMGCPKSFSISGGMGAALLTRPELIHDILTTLKRNLDIPVTCKIRLLKSSQETVELAQRIEKTGVSALAVHGRRIADRPRDPAKWNEIADIVAALSIPVIANGDVFEYDDFQRIKVATGASSVMVARGALWNASVFSPEGKVPWEDVKVEYIRKSILWDNDIKSTKHTLKEMIMHHSCLGLPEGKAVIKSETLADLAKLYGEEKYYQFVRGNRLLLKGSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YIS7</accession>
    <name>A0AA38YIS7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ribonuclease P</fullName>
            <ecNumber evidence="5">3.1.26.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_027275</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.</text>
            <dbReference type="EC" id="3.1.26.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.5" evidence="5"/>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9671210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YIS7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11980">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033443">
        <property type="entry name" value="PROP1-like_PPR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031595">
        <property type="entry name" value="PRORP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13547">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13547:SF1">
        <property type="entry name" value="MITOCHONDRIAL RIBONUCLEASE P CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17177">
        <property type="entry name" value="PPR_long"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16953">
        <property type="entry name" value="PRORP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="13" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PROP1-like PPR" evidence="16">
        <location>
            <begin position="2"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="PRORP" evidence="15">
        <location>
            <begin position="176"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF16953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF17177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9671210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="319" mass="36109" checksum="FD5DDDA726DD55C8" modified="2024-01-24" version="1">MEDPEMAFNLKQLKSCGIPPKLRSYGPPLFGFCKKGDADRAYEVDAHMVESGVVAEEPELCALLGLSVEWRQVDWVYEMMHRLRASVRQVSESTAEVENWDVGKVREGVFKGGGCWHGQGWLGKGKWKVVRTEMDEAGVCQSCGEKLVCIDIDPRETENFASSLTKLACPREVKADFVQFQLNSVVNRLHQISPSKRLPLVILHRSCVTGGPAQNPNNEKLIQSWKKSGALYATPVCSNDNWYWLYAAVNCNCLLVTNDEMRDHLLTMTRRGPVLHMPLPYSIVIQHVPTVTGDDLETPRQWLCATRTRKNHRHPQHLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YJ35</accession>
    <name>A0AA38YJ35_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">folate gamma-glutamyl hydrolase</fullName>
            <ecNumber evidence="3 9">3.4.19.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_025208</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 9">
            <text>(6S)-5,6,7,8-tetrahydrofolyl-(gamma-L-Glu)(n) + (n-1) H2O = (6S)-5,6,7,8-tetrahydrofolate + (n-1) L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:56784"/>
            <dbReference type="Rhea" id="RHEA-COMP:14738"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:141005"/>
            <dbReference type="EC" id="3.4.19.9"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C26 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.9" evidence="3 9"/>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YJ35"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034722">
        <property type="term" value="F:gamma-glutamyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046900">
        <property type="term" value="P:tetrahydrofolylpolyglutamate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000024">
        <property type="entry name" value="Folate gamma-glutamyl hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015527">
        <property type="entry name" value="Pept_C26_g-glut_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011697">
        <property type="entry name" value="Peptidase_C26"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11315:SF0">
        <property type="entry name" value="FOLATE GAMMA-GLUTAMYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11315">
        <property type="entry name" value="PROTEASE FAMILY C26 GAMMA-GLUTAMYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07722">
        <property type="entry name" value="Peptidase_C26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51275">
        <property type="entry name" value="PEPTIDASE_C26_GGH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8 9">
        <location>
            <position position="200"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="313"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00051589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR615527-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9671398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="386" mass="42597" checksum="7BE27850052AF39E" modified="2024-01-24" version="1">MPAHFLSSSPSASTSSTASPADSSDMPKDAVSASSSSSSSGRYSEIWNYLWVPLLLSVSKELTAAKAESTILLPSDVRLGSARSSPSCPAPDPKLNFRPVVGILTHPGDGASGRLNNDTNASYIAASYVKFVESAGARVIPLIYNEPLEILHEKLNLVNGVIFTGGWAKSGLYYKTVGEIFKKSLEKNDVGDHFPVLAICLGFELLTMIISKDQKILEEFSAADQASTLKFMENTDIRGTVFQRFPPDLLRKLSTDCLVMQNHHYGISPERFQENIDLSSFFKILTTSTDEDDKVYVSTVHARSYPVTAFQWHPEKNAFEWGLSRIPHSEDAVQVTQHVANFFISEARKSLNRPPARKVLDNLIYNYSPTYCGKAGKGYDEVYIFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YKW8</accession>
    <name>A0AA38YKW8_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_025811</name>
        <name evidence="2" type="ORF">PVL29_025815</name>
        <name evidence="3" type="ORF">PVL29_025818</name>
    </gene>
    <organism evidence="1 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YKW8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9672343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9672347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9672350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="63" mass="6975" checksum="01253D3871E65EAE" modified="2024-01-24" version="1">MPRHTRKLISFSVGLGEDKSQEKFPSTISIAVSELPFGSLSITSLGRSSRRRKDAILSRETLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YMI0</accession>
    <name>A0AA38YMI0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glycosyltransferase family 92 protein</fullName>
            <ecNumber evidence="8">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_023043</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the glycosyltransferase 92 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="8"/>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YMI0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008166">
        <property type="entry name" value="Glyco_transf_92"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21461:SF56">
        <property type="entry name" value="GALACTAN BETA-1,4-GALACTOSYLTRANSFERASE GALS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21461">
        <property type="entry name" value="GLYCOSYLTRANSFERASE FAMILY 92 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01697">
        <property type="entry name" value="Glyco_transf_92"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="83"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9673201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="508" mass="57502" checksum="C4EC7776190CE04A" modified="2024-01-24" version="1">MRKDASPATVPGNSVGKLFLCFETKPLVATLLALTLVMVIWNLQPYYENLISTTNRCSAAAAAAAATSTSTTIATGNASILPYTPSSSSSSSVSEKKVSTPKASSNADPNKRLFYAYGNAASLFVLMGAYRGGPRTFAVVGLASKPLHVYGRPWYKCEWISSNGSASLRAKAYKMLPDWGYGRVYTVVVVNCTFAENPNEDNAGGKLVLRAYYGESPRKFEKFTVLEEQPGSYNESNYRPPYKYEYLYCGSSLYGNLSAARIREWMAYHAWFFGPKSHFVFHDAGGVSPAVRAALEPWVRAGWATIQDIRGQAEFDGYYYNQFLVVNDCLHRYRHAANWTFYFDVDEYIYLPDGSTLESVLEEFSDYTQFTIEQNPMSSVLCLNDSSRNYSREWGFEKLLFRDSRTRIRRDRKYAIQAKNAYSTGVHMSENVIGKTLHKTETKIRYYHYHNSISVKGEPCREFLPISAKNNVTWFDKLPYVYDDNMTKVANTIKQFERNTIGSIHVFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YML9</accession>
    <name>A0AA38YML9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein MULTIPLE CHLOROPLAST DIVISION SITE 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_023077</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YML9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009706">
        <property type="term" value="C:chloroplast inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010020">
        <property type="term" value="P:chloroplast fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034572">
        <property type="entry name" value="MCD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36317">
        <property type="entry name" value="PROTEIN MULTIPLE CHLOROPLAST DIVISION SITE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36317:SF1">
        <property type="entry name" value="PROTEIN MULTIPLE CHLOROPLAST DIVISION SITE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="105"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="303"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="317"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9673261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="335" mass="37825" checksum="0FAB5FD6C31AC02E" modified="2024-01-24" version="1">MASVWNPQFHSISSQPSICGWKHGISSNILELRRELGLSYSRYNWVQQRTARSFLFRALSKSANSDEDKVVKTETEINGWKTSIGKLQETIKSLPPVVFVMKKDVGINFAIGLCVATTILVVAVRVYVARKVSNSRPGSVADLVRRGQLRSDRRGISRPLKYEDPFNNPLVKVGKSNSTVEMCGKVYRLAPVTLTKEQQAIHQKRRSRAYQWKRPTMFLKEGDSIPSDVDPDTVRWIPANHPFATTASEIDEGLAQNNVYQKHGVPFRIQAEHEALQRKLEALQSEQKLNKLVIDPTNAKDFERPFKHLKSHEQAGQGPSNHIGSDSSPEEMQKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YQK3</accession>
    <name>A0AA38YQK3_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_024022</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YQK3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048437">
        <property type="term" value="P:floral organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00265">
        <property type="entry name" value="MADS_MEF2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1810.10:FF:000016">
        <property type="entry name" value="MADS-box transcription factor 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050142">
        <property type="entry name" value="MADS-box/MEF2_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033896">
        <property type="entry name" value="MEF2-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002487">
        <property type="entry name" value="TF_Kbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48019">
        <property type="entry name" value="SERUM RESPONSE FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01486">
        <property type="entry name" value="K-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00404">
        <property type="entry name" value="MADSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51297">
        <property type="entry name" value="K_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00350">
        <property type="entry name" value="MADS_BOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0287">Flowering</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="8">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="K-box" evidence="9">
        <location>
            <begin position="84"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="84"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9674841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="226" mass="26224" checksum="8617F0B1F6CCAB0A" modified="2024-01-24" version="1">MARGKIEIKRIENSTNRQVTYSKRRNGIFKKASELTVLCDAKVSIIMLSSTGKLHEYISPSTTTKQVFDQYQNTLGVDLWSYHYERMQENLKKLKDVNKNLRKEIRQRMGEHLSDLSVEELRDLQQEMESSLKMVRDRKYQVINNQIETYKKKVRNVEQIHRNLLHEFDARDRDQHYGLVDDGGDYESVLGFSNGSSPVFALSLQPNPPNDLHSGVGSDLTFTLLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YRK0</accession>
    <name>A0AA38YRK0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Serine decarboxylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_024262</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-serine + H(+) = ethanolamine + CO2</text>
            <dbReference type="Rhea" id="RHEA:45824"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57603"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7 8">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the group II decarboxylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YRK0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000069">
        <property type="entry name" value="Serine decarboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051151">
        <property type="entry name" value="Group_II_Decarboxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021115">
        <property type="entry name" value="Pyridoxal-P_BS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002748">
        <property type="entry name" value="PRK02769.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46101">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46101:SF2">
        <property type="entry name" value="SERINE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00392">
        <property type="entry name" value="DDC_GAD_HDC_YDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="5 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 7 8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="7">
        <location>
            <position position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00050312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR602129-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9675237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="442" mass="49608" checksum="92B91811448BE1D0" modified="2024-01-24" version="1">MVGSALVEAMSGDLGGKVEILAEDFDPTAVVVEPVPPVVENGGEMGREEEEKRSKEIVLGRNVHTTCYPYNLDFDYGALSQLQHFSINNLGDPFIESNYGVHSRQFEVGVLDWFARLWEIEKDEYWGYITNCGTEGNLHGILVGREVLPDGILYASQETHYSVFKAARMYRMECVKVATSLSGEIDCADFKAKLLANKDKPAIINVNIGTTVKGAVDDLDLVIETLEECGFTHDRFYIHCDGALFGLMMPFVKRAPKVTFKKPIGSVSVSGHKFVGCPMPCGVQITRMEHVNALSRNVEYLASRDATIMGSRNGHAPIFLWYTLNRKGYKGFQKEVQKCLRNAHYLKDRLRDAGISAMLNELSSTVVFERPVDDEFVRRWQLACQGNIAHVVVMPNVTIEKLDYFLDELIEKRNTWFLDKKVQPPCVAADIGSENCFCALHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YRT7</accession>
    <name>A0AA38YRT7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PB1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_024325</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YRT7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000270">
        <property type="entry name" value="PB1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20930">
        <property type="entry name" value="OVARIAN CARCINOMA ANTIGEN CA125-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20930:SF0">
        <property type="entry name" value="PROTEIN ILRUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00564">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00666">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PB1" evidence="1">
        <location>
            <begin position="4"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9675329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="107" mass="11919" checksum="7AC00ED20C5A8C2A" modified="2024-01-24" version="1">MESTKVIKVKYGNTLRRFNVCVDENGQLDLDINGLRAKVITLFNLVLDADLTLTCIDEDGDVVTLVGDEDLHDVMRQRMKVLRITVLLNIGKDGRSHTRSSGSSTPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YTW3</accession>
    <name>A0AA38YTW3_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_021887</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9676593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YTW3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31903">
        <property type="entry name" value="F12F1.11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31903:SF4">
        <property type="entry name" value="OS11G0490300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="136"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9676593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="211" mass="24410" checksum="F4BDB13C04AA5B61" modified="2024-01-24" version="1">MKIKRKGKVYPSSSSSSSSYKDAISVFKLLPATILALASALSVDDREVLAYMITRSMTTANPSSIIERQKKKCKTPHNVQKLPLFECGCFDCYTSFWFRWDSSPDRELIHHAIEAFEDQLTTTEFFKKNGRGRKRDKLVGRRDAERSADISEEKRTEPEPLKRRRHFPFSVFSIFFNIGQWPFALGRTQLRMVENGSKMWANDISLWGALW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YV95</accession>
    <name>A0AA38YV95_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_022329</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YV95"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009500">
        <property type="entry name" value="DUF1118"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06549">
        <property type="entry name" value="DUF1118"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="175"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9677269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="202" mass="20889" checksum="37F20F44B5AF3B7A" modified="2024-01-24" version="1">MEVRAIAFGGSSRGLSSSSSASFITSIQKPPPPTFSPLLIHSMATQKPTLPPASKTISSRKASTVFPLGEKGPRSSPLATAPPIKVLTRVEQLKLLTKAEKAGLLSAAEKYGFSLSSIEKLGLLSKAEELGILSAATDSGTPGALLSLSLVLLLLGPSCAFLVPEDYPWEVALQLVVAVVCVVGGSAAFAASNFVSNLQKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YY19</accession>
    <name>A0AA38YY19_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_020815</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the AP2/ERF transcription factor family. ERF subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YY19"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009873">
        <property type="term" value="P:ethylene-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000001">
        <property type="entry name" value="Ethylene-responsive transcription factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044808">
        <property type="entry name" value="ERF_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31190">
        <property type="entry name" value="DNA-BINDING DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31190:SF499">
        <property type="entry name" value="ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR ERF105"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00367">
        <property type="entry name" value="ETHRSPELEMNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0936">Ethylene signaling pathway</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="10">
        <location>
            <begin position="119"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="82"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="184"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="96"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="194"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9678739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="277" mass="30810" checksum="D235B6C5671BE36A" modified="2024-01-24" version="1">MAEEASSLHFIHQQLFFDFEAFESFVSHVDDPSQTSTSDSSASTSDINPLSNYFNPHEDENNPFLVHCSTSAPPGLFQFQTKSPKTSTLSHRRSPLSISVPQPAVSQSPAESDSGDIRHYRGVRRRPWGKFVAEIRDPNRRGSRAWLGTFETAIEAARAYDRAAFKMRGSKAILNFPLEADNWSGSDPPAISGRKRERDSETAERQQVEIKVSKQDEHLPESDGTLAAASNVLGVSPLTPSNFRAGWEETDMEGIFHLPPLTPLSPHPWIAYSQLMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YYG2</accession>
    <name>A0AA38YYG2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myb/SANT-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_021009</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YYG2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024752">
        <property type="entry name" value="Myb/SANT-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46929">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46929:SF3">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12776">
        <property type="entry name" value="Myb_DNA-bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb/SANT-like" evidence="2">
        <location>
            <begin position="50"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9678964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="108" mass="12411" checksum="E071931E5CAF41D7" modified="2024-01-24" version="1">MDEVRSSNQIPMDEMNSSNPVNVGSMSSDQMEHSEGFEVHKSVSEKRNLTWIDEMDNFLVDRLMEQMHKGQKIGRVFAKTAYAIVAQEIGENFELSCNSEHIKNRMKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YZK2</accession>
    <name>A0AA38YZK2_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_021523</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YZK2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9679623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="51" mass="5766" checksum="6200063E589B4DCE" modified="2024-01-24" version="1">MHGGAPSPSWRDDQPLKPGVVKLLRRKAYISTLISAMLAFQALRHKALQFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YZR9</accession>
    <name>A0AA38YZR9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Reticulon-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_021513</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Endoplasmic reticulum membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YZR9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009617">
        <property type="term" value="P:response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003388">
        <property type="entry name" value="Reticulon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045064">
        <property type="entry name" value="Reticulon-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10994">
        <property type="entry name" value="RETICULON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10994:SF193">
        <property type="entry name" value="RETICULON-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02453">
        <property type="entry name" value="Reticulon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50845">
        <property type="entry name" value="RETICULON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="79"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="104"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="192"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="Reticulon" evidence="7">
        <location>
            <begin position="68"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9679610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="254" mass="28123" checksum="27F8361B49355DBF" modified="2024-01-24" version="1">MADHAGESESAMESVMEKITAKLHGHDSQSSSDSEDGKKSLVEAVKSKSYRLFGREKPLHKVLGGGKPADVFLWRNKKISGSVLGCATAIWVLFEMLEYQLLPLLCHILMLCLASLFLWSNASIVIKKSPPNIPVVGVPEKAVMEITSALLFAINRGLTSLHNIASGRHLKQFLAVTAGLWVLSAVGRWCNFITLFYLLFVLLHTVPVIYEKYEDEVDSSAEKVKTGIKKQYRVLCEKVLKGQPGDVLKVRKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z0S1</accession>
    <name>A0AA38Z0S1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">CDC20/Fizzy WD40 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_019550</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin-protein ligase complex that controls progression through mitosis and the G1 phase of the cell cycle.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat CDC20/Fizzy family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z0S1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010997">
        <property type="term" value="F:anaphase-promoting complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990757">
        <property type="term" value="F:ubiquitin ligase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905786">
        <property type="term" value="P:positive regulation of anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033010">
        <property type="entry name" value="Cdc20/Fizzy"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056150">
        <property type="entry name" value="WD40_CDC20-Fz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918">
        <property type="entry name" value="CELL DIVISION CYCLE 20 CDC20 FIZZY -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918:SF8">
        <property type="entry name" value="FI02843P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24807">
        <property type="entry name" value="WD40_CDC20-Fz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 8" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="CDC20/Fizzy WD40" evidence="10">
        <location>
            <begin position="125"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="171"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="255"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="391"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF24807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9680271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="450" mass="49643" checksum="BADC1A20A4A662E6" modified="2024-01-24" version="1">MDAGSLTSSNKYQSKCPDQRRTVRENLDRFIPNRSAMDFDYAHYMLTEGRKGKENPAASSPSKEAYRKQMAETLNINRTRILAFKNKPPTPVELIPQEFYSASIPQQSKTSKPRRHIPQTSERTLDAPDLVDDYYLNLLDWGSSNVLAIALGGTVYLWDASDGSTSELVTLEDESGPVTSVSWAPDGRHIGIGLNNSDVQLWDSTANRLLRTLKGGHASRVGSLAWNNHVLTTGGMDGKIINNDVRVRSHIVETYRGHRQEVCGLKWSASGQQLASGGNDNLLYVWDKSSASSNSPSQWLHRMEDHTAAVKALAWCPFQGNLLASGGGGGDRCIKFWNTHTGACLNSVDTGSQVCALLWNKNERELLSSHGFTQNQLTLWKYPSMVKMAELTGHTSRVLFMAQSPDGCTVASAAGDETLRFWNVFGTPEVAAKPAPKAHPEPFAHLNRIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z1U8</accession>
    <name>A0AA38Z1U8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Subtilisin-like protease SBT1.1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_019974</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the peptidase S8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z1U8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02120">
        <property type="entry name" value="PA_subtilisin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04852">
        <property type="entry name" value="Peptidases_S8_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.2310:FF:000001">
        <property type="entry name" value="Subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000006">
        <property type="entry name" value="Subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000005">
        <property type="entry name" value="subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034197">
        <property type="entry name" value="Peptidases_S8_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010259">
        <property type="entry name" value="S8pro/Inhibitor_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045051">
        <property type="entry name" value="SBT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041469">
        <property type="entry name" value="Subtilisin-like_FN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10795">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17766">
        <property type="entry name" value="fn3_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05922">
        <property type="entry name" value="Inhibitor_I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 7" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041471033" description="Subtilisin-like protease SBT1.1" evidence="9">
        <location>
            <begin position="21"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="domain" description="Inhibitor I9" evidence="12">
        <location>
            <begin position="27"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="10">
        <location>
            <begin position="141"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="11">
        <location>
            <begin position="378"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="Subtilisin-like protease fibronectin type-III" evidence="13">
        <location>
            <begin position="664"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="589"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="592"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6 7">
        <location>
            <position position="149"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6 7">
        <location>
            <position position="223"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6 7">
        <location>
            <position position="548"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF05922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF17766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9680821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="768" mass="80925" checksum="78601638AD29CE0D" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z327</accession>
    <name>A0AA38Z327_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Clu domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_020198</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9681207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019750">
        <property type="term" value="P:chloroplast localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd15466">
        <property type="entry name" value="CLU-central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000024">
        <property type="entry name" value="Tetratricopeptide repeat (TPR)-like superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033646">
        <property type="entry name" value="CLU-central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025697">
        <property type="entry name" value="CLU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028275">
        <property type="entry name" value="CLU_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027523">
        <property type="entry name" value="CLU_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12601">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT EIF-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12601:SF17">
        <property type="entry name" value="PROTEIN REDUCED CHLOROPLAST COVERAGE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15044">
        <property type="entry name" value="CLU_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12807">
        <property type="entry name" value="eIF3_p135"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51823">
        <property type="entry name" value="CLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 7" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="Clu" evidence="9">
        <location>
            <begin position="310"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="925"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="126"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="578"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1170"/>
            <end position="1260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1400"/>
            <end position="1420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1491"/>
            <end position="1525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1540"/>
            <end position="1563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="138"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="580"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1177"/>
            <end position="1193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1199"/>
            <end position="1209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1224"/>
            <end position="1237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1514"/>
            <end position="1524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9681207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1847" mass="202917" checksum="6CB90FECAC24F93E" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z683</accession>
    <name>A0AA38Z683_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_018951</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z683"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase zinc-binding" evidence="1">
        <location>
            <begin position="2"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9683166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="114" mass="13759" checksum="739134AB375A6D08" modified="2024-01-24" version="1">MRRGWSMVNRCNLCKENEESANHILIHCGKTRELWTLLLSSFGVVWVFPDSVRNLLLEWKIKGLGKKRSIVWRMAPICLFWCIWGEQNRRTFQEEELSDTRLRKLFLRSLFEWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z6M7</accession>
    <name>A0AA38Z6M7_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_019029</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z6M7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000129">
        <property type="entry name" value="Leucine-rich repeat receptor-like kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000213">
        <property type="entry name" value="Tyrosine-sulfated glycopeptide receptor 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046956">
        <property type="entry name" value="RLP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063">
        <property type="entry name" value="LRR RECEPTOR-LIKE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063:SF16">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 14" id="KW-0732">Signal</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041382471" evidence="14">
        <location>
            <begin position="20"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="923"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="15">
        <location>
            <begin position="42"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="16">
        <location>
            <begin position="295"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="890"/>
            <end position="915"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9683269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="988" mass="109421" checksum="551C3FAD250FFB2D" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z7W3</accession>
    <name>A0AA38Z7W3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_019200</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AAA ATPase family. BCS1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z7W3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19510">
        <property type="entry name" value="RecA-like_BCS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001122">
        <property type="entry name" value="AAA-ATPase ASD, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025753">
        <property type="entry name" value="AAA_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058017">
        <property type="entry name" value="At3g28540-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050747">
        <property type="entry name" value="Mitochondrial_chaperone_BCS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23070">
        <property type="entry name" value="BCS1 AAA-TYPE ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14363">
        <property type="entry name" value="AAA_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25568">
        <property type="entry name" value="AAA_lid_At3g28540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="10">
        <location>
            <begin position="239"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="309"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="460"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="322"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="460"/>
            <end position="506"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9683519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="516" mass="59906" checksum="7389EDFCA5DC2D10" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z8Q2</accession>
    <name>A0AA38Z8Q2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Legume lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_016772</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the leguminous lectin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z8Q2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06899">
        <property type="entry name" value="lectin_legume_LecRK_Arcelin_ConA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001220">
        <property type="entry name" value="Legume_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050258">
        <property type="entry name" value="Leguminous_Lectin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32401">
        <property type="entry name" value="CONCANAVALIN A-LIKE LECTIN FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32401:SF15">
        <property type="entry name" value="L-TYPE LECTIN-DOMAIN CONTAINING RECEPTOR KINASE VIII.2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00139">
        <property type="entry name" value="Lectin_legB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0430">Lectin</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041458616" description="Legume lectin domain-containing protein" evidence="4">
        <location>
            <begin position="31"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="279"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="Legume lectin" evidence="5">
        <location>
            <begin position="30"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9684466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="343" mass="37622" checksum="5AC12F5E0F1B842A" modified="2024-01-24" version="1">MPTFSFPSYSSTLFPLAFCFLALSAKPISAFSFQDFRKTSFDSEIALYGDARISNDGSSVTLTPPLASSFGLLAYNRGFRLLEATSFSTDFSFSISPDNGDGLALVVLPNDFALKLDSSAFGLSRKIRFLGVEFDTSMDENVGDPNGNHVGIDVGSLVSASVRNLSSANLLLNSGVKLRSWIDYNANSKRLEVWLGKLGSSRPNDPILSYPIDLSKMWKGEEVFVGISASSGNSSQTSSVYSWSFRLRSVPNWLHSQPVDPRRERGEPLRMQKSSACPLTVLAGVIFSTGCLALMAFVLLFVWTIFVARHTTVAAAATEEEYPKFPVDFRYQKIDVVDTKDVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZA13</accession>
    <name>A0AA38ZA13_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_017143</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZA13"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11072">
        <property type="entry name" value="CYP71-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000011">
        <property type="entry name" value="Cytochrome P450 83B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47955:SF22">
        <property type="entry name" value="CYTOCHROME P450 83B1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47955">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 71 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12 13" id="KW-0349">Heme</keyword>
    <keyword evidence="9 12 13" id="KW-0408">Iron</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 13" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="8 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="binding site" description="axial binding residue" evidence="12">
        <location>
            <position position="374"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9684995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="433" mass="49421" checksum="110CE8AADA6E54B6" modified="2024-01-24" version="1">MILRLGFVPTLVVSSARMAKEVMKTHDLEFSGRPSLLGLQKLSYNGLDVAFSPYNDYWREMKKICVLHLFNSKRAQSFRPIREDEVLEMIKKISQFASASKLTNLSEILISLTSTIICRVAFSKRYDDEGCERSRFQKLVGEGQAVVGGFYFSDHFPVMGWVDKLTGMIARAEENFKEFDLFYQEIIDEHLDPNRPEPEKEDITDVLLKLRKNRLFTIDLTFDHIKAVLMNIFLPGTDTSAATVVWAMTMLMKNPTTMKKAQEEIRNLIGKKCFVDEDDLQKLPYLKAIVKETMRLHPASPLLVPRETLEKCVIDGYEIPPKILVYVNAWAIGRDPESWENPEEFTPERFLGTSIDFKGQDYQLIPFGGGRRICPGLNLGAAVVELKLANLLYSFDWEMPAGMNKEDIDINVKPGVTMHKKNALCLLARIPSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZAJ7</accession>
    <name>A0AA38ZAJ7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Disease resistance RPP13-like protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_017474</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056789">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766:SF51">
        <property type="entry name" value="DISEASE RESISTANCE RPP13-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766">
        <property type="entry name" value="PLANT BROAD-SPECTRUM MILDEW RESISTANCE PROTEIN RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25019">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0611">Plant defense</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="7">
        <location>
            <begin position="11"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="6">
        <location>
            <begin position="181"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="8">
        <location>
            <begin position="437"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="domain" description="R13L1/DRL21-like LRR repeat region" evidence="9">
        <location>
            <begin position="696"/>
            <end position="820"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF25019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9685446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1324" mass="150364" checksum="76BA816359D698A4" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZAU2</accession>
    <name>A0AA38ZAU2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Annexin 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_017492</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZAU2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009409">
        <property type="term" value="P:response to cold"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009651">
        <property type="term" value="P:response to salt stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009414">
        <property type="term" value="P:response to water deprivation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000006">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000014">
        <property type="entry name" value="annexin D4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000021">
        <property type="entry name" value="annexin D4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.10">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001464">
        <property type="entry name" value="Annexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018502">
        <property type="entry name" value="Annexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037104">
        <property type="entry name" value="Annexin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10502">
        <property type="entry name" value="ANNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10502:SF196">
        <property type="entry name" value="ANNEXIN D4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00191">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00196">
        <property type="entry name" value="ANNEXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00335">
        <property type="entry name" value="ANX"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47874">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0041">Annexin</keyword>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0111">Calcium/phospholipid-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9685474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="314" mass="36206" checksum="1ABB637028D15EF5" modified="2024-01-24" version="1">MALSHEFQALTKSFSGFGVDEKSMISILGKWHQDDRKSYRKGCPQFFIQDDRLFEKWDDRHVAFLKHEFLRLKNAVVLWTMHPWERDARLMKEALVKGPQAYGVIIEVASTRSSEQLLGARRAYHSLFDHSIEEDVAYHINDSRRKLLVGLVSSYRYEGPKVNEEIAKSEAKTLFAAIKNADKKNPIEDEEVVRILTTRSKPHLKVIFKHYKEINGKNIDEDLDDELSLDETMQCLCTPQTYFSKVLGAAFQNGADEHAKEALTRVIVTRADDDMKEIKEEYQKKYGVSLSKKIEDAVNGNYKDFLLTLIARGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZCF8</accession>
    <name>A0AA38ZCF8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cellulose synthase-like protein H1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_015390</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZCF8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016760">
        <property type="term" value="F:cellulose synthase (UDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030244">
        <property type="term" value="P:cellulose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000145">
        <property type="entry name" value="Cellulose synthase-like protein H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005150">
        <property type="entry name" value="Cellulose_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13301">
        <property type="entry name" value="X-BOX TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03552">
        <property type="entry name" value="Cellulose_synt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="574"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="613"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="667"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="702"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="732"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="185"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="136"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="464"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="271"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR605150-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR605150-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR605150-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9686466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="751" mass="84359" checksum="36BFEB4AF2792CC0" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZET4</accession>
    <name>A0AA38ZET4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Chloride channel protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_016264</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the chloride channel (TC 2.A.49) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZET4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009705">
        <property type="term" value="C:plant-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005247">
        <property type="term" value="F:voltage-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04591">
        <property type="entry name" value="CBS_pair_voltage-gated_CLC_euk_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03685">
        <property type="entry name" value="ClC_6_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3080.10:FF:000004">
        <property type="entry name" value="Chloride channel ClC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3080.10">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051280">
        <property type="entry name" value="Cl-channel/antiporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014743">
        <property type="entry name" value="Cl-channel_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002251">
        <property type="entry name" value="Cl_channel_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001807">
        <property type="entry name" value="ClC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11689:SF144">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11689">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN CLC FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00654">
        <property type="entry name" value="Voltage_CLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00762">
        <property type="entry name" value="CLCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01120">
        <property type="entry name" value="CLCHANNELPLT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81340">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0129">CBS domain</keyword>
    <keyword evidence="12 14" id="KW-0868">Chloride</keyword>
    <keyword evidence="11" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="6" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 14" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="90"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="136"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="183"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="252"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="287"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="339"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="379"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="461"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="547"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="15">
        <location>
            <begin position="711"/>
            <end position="775"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9687706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="777" mass="84930" checksum="7202AA760887F23C" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZFP2</accession>
    <name>A0AA38ZFP2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_016165</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DExH box helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd17917">
        <property type="entry name" value="DEXHc_RHA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000819">
        <property type="entry name" value="ATP dependent RNA helicase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000002">
        <property type="entry name" value="Putative ATP-dependent RNA helicase DHX36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056328">
        <property type="entry name" value="DSRM_DHX29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059023">
        <property type="entry name" value="RNA_hel_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056890">
        <property type="entry name" value="UBA_DHX29-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF246">
        <property type="entry name" value="DEXH-BOX ATP-DEPENDENT RNA HELICASE DEXH4, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24385">
        <property type="entry name" value="DSRM_DHX29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26026">
        <property type="entry name" value="RNA_hel_CTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24899">
        <property type="entry name" value="UBA_DHX29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="624"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="871"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="9"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="42"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00060772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9687568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1399" mass="155294" checksum="C4BA95F0E0702AEA" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZHG6</accession>
    <name>A0AA38ZHG6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF4283 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_014512</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZHG6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="173"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="246"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="305"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="588"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="252"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="333"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="376"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="596"/>
            <end position="608"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9688895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="649" mass="71704" checksum="25597FB138FE506D" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZKE5</accession>
    <name>A0AA38ZKE5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rho termination factor-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_013069</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZKE5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011112">
        <property type="entry name" value="Rho-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036269">
        <property type="entry name" value="Rho_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34449">
        <property type="entry name" value="RHO TERMINATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34449:SF2">
        <property type="entry name" value="RHO TERMINATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07498">
        <property type="entry name" value="Rho_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68912">
        <property type="entry name" value="Rho N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho termination factor-like N-terminal" evidence="2">
        <location>
            <begin position="228"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="143"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9690726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="261" mass="28980" checksum="0193E9F63CD4292F" modified="2024-01-24" version="1">MVAVVFCSHSALRLQSFYAFSKPKFGKSAVSLQETADGFSPFASRRDLPLTISSIKSDGNRRGRPPRKNHISGRTEKGDKNKTPPSSDGKLSESSNQDEIIALFRRIQSSISKGESLGTKKRISDSSVDKTSAESVLEILRQSRKQVKGRPSKKEGDQVLTQRRGVPKKDQGIPDKQYVADLKSARPPSNFVKRSPIPSHTTPRGKAVKLQNEVSVRTISFNELPKVEEMKVTELKELAKSRGIKGYSKMKKSELVELLRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZKN5</accession>
    <name>A0AA38ZKN5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_013151</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZKN5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.330:FF:000001">
        <property type="entry name" value="Protein SRG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050295">
        <property type="entry name" value="Plant_2OG-oxidoreductases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47991">
        <property type="entry name" value="OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="6">
        <location>
            <begin position="205"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9690856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="362" mass="40677" checksum="B55D3AB1C466817F" modified="2024-01-24" version="1">MASTQSSSFRAVPIQSVQELIKEPIPAVPQPYILDDPQPPILSASTPLPLLPTIDMKHLIMGETAGSELEKLHSTCKEWGFFQLVNHGVSSSLVEKLKSEIGEFYKLPLEERMKYKMRPGDVEGYGHLPIRSEDQKLDWGDRFYMITNPIHTRKPYLLPELPSSLRDSLECYLAELQKLAMLLLGSMAKALKLEKGEMEELFEDGMQSVRMTYYPPCPQPELVMGLTPHSDASGITILLQINGVDGLQIKKDGVWIPVNILPDALVVNVGDVLEILSNGVYTSIEHRATVNAAKERISIAMFFNPKFSAQIKPAPSLINPQNPPLFKEVGMEKYCKDFFSRKLDGKSYLEHMKIKNAEEYST</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZLE1</accession>
    <name>A0AA38ZLE1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="25">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">PVL29_013425</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="25">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Serine/threonine-protein kinase that may function as a signaling receptor of extracellular matrix component. Binding to pectin may have significance in the control of cell expansion, morphogenesis and development.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="19">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="25">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZLE1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000038">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000084">
        <property type="entry name" value="Wall-associated receptor kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000628">
        <property type="entry name" value="Wall-associated receptor kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000043">
        <property type="entry name" value="Wall-associated receptor kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045274">
        <property type="entry name" value="WAK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025287">
        <property type="entry name" value="WAK_GUB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27005">
        <property type="entry name" value="WALL-ASSOCIATED RECEPTOR KINASE-LIKE 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27005:SF353">
        <property type="entry name" value="WALL-ASSOCIATED RECEPTOR KINASE-LIKE 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13947">
        <property type="entry name" value="GUB_WAK_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 19" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="13 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7 22" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041342185" evidence="22">
        <location>
            <begin position="31"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="408"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="24">
        <location>
            <begin position="353"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="484"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="513"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00058961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="KAJ9691246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="757" mass="83810" checksum="51F1C16DCBDD4D0A" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZNX0</accession>
    <name>A0AA38ZNX0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Late embryogenesis abundant protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_011558</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZNX0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039624">
        <property type="entry name" value="LEA1/2/D7/KIN2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34191:SF9">
        <property type="entry name" value="F6D8.10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34191">
        <property type="entry name" value="LATE EMBRYOGENESIS ABUNDANT PROTEIN (LEA) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="72"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9692566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="99" mass="10526" checksum="D6F0FE0271ABBC54" modified="2024-01-24" version="1">MASQQQPRNLSQSDQICQDGPCEQNASYTQQASNFLQQTGEQVKNMAQGAAEAVKNTLGMNNENADNSVTANLPSNTSNPSNLSNPSNPSNLSNPTTKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZPG4</accession>
    <name>A0AA38ZPG4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">G-patch domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_011205</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9691993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZPG4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000467">
        <property type="entry name" value="G_patch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045166">
        <property type="entry name" value="Spp2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026822">
        <property type="entry name" value="Spp2/MOS2_G-patch"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15818">
        <property type="entry name" value="G PATCH AND KOW-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15818:SF2">
        <property type="entry name" value="G-PATCH DOMAIN AND KOW MOTIFS-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12656">
        <property type="entry name" value="G-patch_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25088">
        <property type="entry name" value="GPKOW_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50174">
        <property type="entry name" value="G_PATCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G-patch" evidence="4">
        <location>
            <begin position="153"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="200"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="56"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="216"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9691993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="420" mass="47698" checksum="379AC62B8AC3A206" modified="2024-01-24" version="1">MKLSFSLSSSNSKPVSRKPNVKRSDDDAPPHVYVTEFDASKTLADTQTKRLIVPPKENEWRPEKKMKNLDLPLQSTTESTDIRFELESSDAAAGDSSMSYGLNLRENSKSENEVAAANHPDESVEATLLKKLKQDLRRLPDDQTLDDFAEVPVEGFGAALLAGYGWTEGRGIGRNRKEDVKIVEYNRSFAKQGFAFFSSAQKENSDSKIRANNAAESKEDENLKKSKDERRRDEKRDSLSIHRERREKEDKRRREEGPRGERLKHENGLSKKSSSRREEERSSRREEGRSIRREVEKITPWLTSHIRVRVISKDFKGGRFYLKKAEILDVVGPTTCDISMDESRELIQGVDQELLETALPRRGGPVLVLFGKHKGAYGSLVERDSEQETGVVRDADSHALLNVRLEQIAEYVGDPSYIGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZQL1</accession>
    <name>A0AA38ZQL1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_012215</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9693362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZQL1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000440">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025724">
        <property type="entry name" value="GAG-pre-integrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054722">
        <property type="entry name" value="PolX-like_BBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039537">
        <property type="entry name" value="Retrotran_Ty1/copia-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057670">
        <property type="entry name" value="SH3_retrovirus"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648">
        <property type="entry name" value="TRANSPOSASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648:SF28">
        <property type="entry name" value="TRANSPOSON-ENCODED PROTEIN WITH RIBONUCLEASE H-LIKE AND RETROVIRUS ZINC FINGER-LIKE DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13976">
        <property type="entry name" value="gag_pre-integrs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22936">
        <property type="entry name" value="Pol_BBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25597">
        <property type="entry name" value="SH3_retrovirus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="3">
        <location>
            <begin position="321"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="46"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="101"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="47"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="104"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9693362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="583" mass="64313" checksum="1EDBBE8E6CEDF3B4" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZQP3</accession>
    <name>A0AA38ZQP3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Gag1-like clamp domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_011649</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZQP3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025124">
        <property type="entry name" value="Gag1-like_clamp"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33373:SF1">
        <property type="entry name" value="DUF4050 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33373">
        <property type="entry name" value="OS07G0479600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13259">
        <property type="entry name" value="clamp_Gag1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gag1-like clamp" evidence="1">
        <location>
            <begin position="6"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9692668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="109" mass="12268" checksum="899B6621D8861D19" modified="2024-01-24" version="1">MDNSAVQSQGSISSISTSNQILVPHGAAGANVPSEFVNHGLLLWNQTRQNWIRNKRSDNQAQQIQEPKINWNATYDSLLGSNKPFRQPVPLSEMVDFLVDVWEQEGLYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZTQ3</accession>
    <name>A0AA38ZTQ3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_010560</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZTQ3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000270">
        <property type="entry name" value="Alpha/beta-Hydrolases superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45763:SF51">
        <property type="entry name" value="ALPHA_BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45763">
        <property type="entry name" value="HYDROLASE, ALPHA/BETA FOLD FAMILY PROTEIN, EXPRESSED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="120"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9695121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="376" mass="42671" checksum="06C37CF1277FD00F" modified="2024-01-24" version="1">MAGGVNRKISAASARAHTRKSRQSSSFRLPSGMFKKILVVLLMGFLAWAYQAIQSPPPKICGSSDGPPVTASRIKLSDGRHLAYKEHGVPKDRAKYKIVYVHGFDSCRHDAIVATTLSPEIIEELGIYIVSFDRPGYGESDPNPKRTVKSLPLDIEELADQLGLGSKFYVIGFSMGGQVIWSCLKYIPHRLAGATLIAPVVNYWWPSFPANLSKEAYYQQFRQDQWALRVAHYTPWLTYWWNTQKWFPACSVAEHSTDILSRQDKELMPKLSKRKEYMAQVRQQGEFESIHRDLMIGFGTWEFDPMDLKNPFPNNEGSVHLWHGDEDAMVPVSLQRYIAQQLPWIQFHEIPGAGHLFPYAEGRTNAMIKALLTGEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZWN0</accession>
    <name>A0AA38ZWN0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_008736</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZWN0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37172">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37172:SF3">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9696666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="265" mass="29717" checksum="68FCE187361C9C2D" modified="2024-01-24" version="1">MLRETFHILTVTLLSLLLPLSFLLLARLSIAHYLLSLGTNIAIPQPFSLVFSFFLYTNPTLLHALVSIISLATLVHGLTGRMALLSEATSQSFQPRLYTAWIFLCTLQVCVGLGIEVSIAAGIESTSLGRERSLLSRVVFFLGLHETMLHWSRTIVKPVVDDTIFGAAREERWAERVIMASSLGALWWWRLRDEVESLAIVAEMKRDLLMGVGVADFVGWWLYYLTVTIGMVRVVKGLMWVGMVLLCRRKVEQTNGDSCGDEDKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZY13</accession>
    <name>A0AA38ZY13_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_009194</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054722">
        <property type="entry name" value="PolX-like_BBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039537">
        <property type="entry name" value="Retrotran_Ty1/copia-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057670">
        <property type="entry name" value="SH3_retrovirus"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648">
        <property type="entry name" value="TRANSPOSASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648:SF28">
        <property type="entry name" value="TRANSPOSON-ENCODED PROTEIN WITH RIBONUCLEASE H-LIKE AND RETROVIRUS ZINC FINGER-LIKE DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22936">
        <property type="entry name" value="Pol_BBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25597">
        <property type="entry name" value="SH3_retrovirus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="6">
        <location>
            <begin position="490"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="229"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="250"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9697292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1358" mass="154248" checksum="A5159D7420560FB4" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZYB4</accession>
    <name>A0AA38ZYB4_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_009335</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0587 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZYB4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008584">
        <property type="entry name" value="CXXC_Zn-binding_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12857">
        <property type="entry name" value="CXXC MOTIF CONTAINING ZINC BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12857:SF0">
        <property type="entry name" value="CXXC MOTIF CONTAINING ZINC BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05907">
        <property type="entry name" value="CXXC_Zn-b_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9697471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="145" mass="16579" checksum="F4310723AA2B2D6B" modified="2024-01-24" version="1">MPKVMLVVSIEMKNIALLQPRAGSDDTTINYLFKMECEQCSWVSIEEVCVSLGVKEKPIVRSRRRNYNNASLRVGHGNPLSEHHCIEVRLMLFNYDELIPVTYSLNDGWLAVMIHVDLVGQEYRNVIDGEKVTIMNLEAKFVPRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZZT0</accession>
    <name>A0AA38ZZT0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_007341</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZZT0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45763:SF36">
        <property type="entry name" value="AB HYDROLASE-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45763">
        <property type="entry name" value="HYDROLASE, ALPHA/BETA FOLD FAMILY PROTEIN, EXPRESSED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="171"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9698207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="489" mass="55723" checksum="F5DC2B9DD9B8546D" modified="2024-01-24" version="1">MEVRSMEYGNGVSELEAESFKDQLKGFLKASAEMGVELVKGCRDIVKQSLGNENSYVVRKFGGHWNRLSRRLSFLNEFLPEDRDPTHAWSVVLFVSVLAFTALNNTEYATPTPLLKEVYIHPPNASRILLPDGRYLAYQEQGVLAERARYSMMAPHSFLSSRLSGIPGVKAPLLEEFGIRLVTYDLPGFGESDPHPNRNLESSALDMLYLANALGVNGKFWVVGHSGGSMHAWAALRYIPDRLAGAAMFAPVVNPYDPSMTKQERYGTWEKWTFRRKLMYFLARRFPRFLSYLYRKSFLSGKHGQIDKWLSLSLGKRDKALIAEPIFVEFWQRDVEESIRQGDAKPFVEEAVMQVSDWGFSLGELKMQKKHRGSGILHWLKSKYSQEEEELMGFLGPIHIWQGMDDRVVPPSMTDFVHRILPGATIHKLPYEGHFTYFYFCDKCHRQIFNTLFETPQGPLVTIEEVDQTPIEGGVEDAEEVTLGDSATK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A083</accession>
    <name>A0AA39A083_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_007232</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A083"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.80">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003441">
        <property type="entry name" value="NAC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036093">
        <property type="entry name" value="NAC_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31719">
        <property type="entry name" value="NAC TRANSCRIPTION FACTOR 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31719:SF179">
        <property type="entry name" value="OS08G0148400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02365">
        <property type="entry name" value="NAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101941">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51005">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NAC" evidence="6">
        <location>
            <begin position="41"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="197"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="197"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9698022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="381" mass="43280" checksum="CC7A87A94CE8D2E4" modified="2024-01-24" version="1">MVGGTQVPVGGSRIPKPEEEEKEIRILDYRFKNKDEFLNSMPPGYNFSPTDEEVILYFLRPKIENMPLPINRIMEVNIYQYDPEDLTALFPPCGNHNDGIDQWFFFTPRDRKYPNGKRPSRRAGKGYWKATGGDKAIKCENQTIGYRKVLVFYRGKAPKGEKTIWLMHEFRGKEPPRSPSANIMRLDIVLSKIYKKPNKPLTRRSSTHAPEPEPEPPHPVKVEAGGMEQNGDQTRLFTLGTGQELNQIISYVDPYEEQCFYNSSQMPLQTQQPQPTTEIFQTPQEHDNFALQSNTQFPILDPSVFMPLPTLHQPIHSIGNEFGGRGLDNFDIMNGAFGNSSFDAGNNIDFGNPGLYPMINYGNDAAPMSSNMNGNYGRHLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A146</accession>
    <name>A0AA39A146_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_007194</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9697964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A146"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36586:SF43">
        <property type="entry name" value="EXTENSIN-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36586">
        <property type="entry name" value="PROLINE-RICH EXTENSIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041395480" evidence="2">
        <location>
            <begin position="24"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="163"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="294"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="407"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9697964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="531" mass="57430" checksum="1F7F3FE9C5058096" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A2X0</accession>
    <name>A0AA39A2X0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Aspartic proteinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_005691</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the peptidase A1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9699975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A2X0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06098">
        <property type="entry name" value="phytepsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000009">
        <property type="entry name" value="Aspartic proteinase A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000044">
        <property type="entry name" value="Lysosomal aspartic protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.225.10">
        <property type="entry name" value="Saposin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033869">
        <property type="entry name" value="Phytepsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007856">
        <property type="entry name" value="SapB_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008138">
        <property type="entry name" value="SapB_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011001">
        <property type="entry name" value="Saposin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008139">
        <property type="entry name" value="SaposinB_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF20">
        <property type="entry name" value="ASPARTIC PROTEINASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05184">
        <property type="entry name" value="SapB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03489">
        <property type="entry name" value="SapB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47862">
        <property type="entry name" value="Saposin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50015">
        <property type="entry name" value="SAP_B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="7 10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 11" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041354553" description="Aspartic proteinase" evidence="12">
        <location>
            <begin position="22"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="14">
        <location>
            <begin position="83"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="domain" description="Saposin B-type" evidence="13">
        <location>
            <begin position="313"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="Saposin B-type" evidence="13">
        <location>
            <begin position="369"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="101"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="288"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="114"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="279"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9699975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="500" mass="54874" checksum="0201CBD73E42B574" modified="2024-01-24" version="1">MRLKYLLVANCLLWAWACCLALDDSSDGLVRIGLKKKPLDLARLHAARITRGNGFHARGLGKVDDNYPKANTVYLKNYMDAQYYGEIGIGSPPQTFSVVFDTGSSNLWVPSSKCYFSIACYFHARYRAVLSRTYSKNGRHCKINYGSGSISGFFSQDHVQIGEIVIKNQVFTEATKEGLFAFSLAQFDGILGLGFQNASVGKIPPIWYNMVQQSLVSMEIVSFWLNRDPKAKIGGEVIFGGVDWRHFMGDHTFVPITRKDYWQIEVGDILIAGSSTGFCEGGCAAIVDTGTSLIAGPTTVVTQINHAIGAEGIVSFNCKNVVNKYGQLIWQFLVSGFQPENVCSDVGLCAYNGTKNASAGMETVPGNGENAACTFCEMIAFWIQVQLKEHKAKEKVFQYVNELCENLPNPGGKDFVNCDALATMPVISFAIGDKYFPLTAEQYTLRVEVNCTTVCLSGFTALDVPRPDGPLWVLGDVFLGAYHTIFDFGNLQVGFAKSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A3T6</accession>
    <name>A0AA39A3T6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_005868</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A3T6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00268">
        <property type="entry name" value="DEADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044742">
        <property type="entry name" value="DEAD/DEAH_RhlB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050547">
        <property type="entry name" value="DEAD_box_RNA_helicases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47963:SF10">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DDX6_DHH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47963">
        <property type="entry name" value="DEAD-BOX ATP-DEPENDENT RNA HELICASE 47, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="110"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="8">
        <location>
            <begin position="321"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9700249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="481" mass="53933" checksum="A16330CC33E45AD3" modified="2024-01-24" version="1">MASLPTAQFLSLFRTFSGKSCPPRALAPSLNSNRRTVATIHFTQRVSKSNFNAKPAQATLNSSFIIEASDTGERTLTLREICQGCVPEHVLRRIEEVGFIVPTDVQQQALPVLLSGRDCILHAQTGSGKTLTYLLLIFSVVSTKKSAVQALIVVPTRELGIQVTKVARMLAAKPMEPELEQKSCTVMALLDGGMLKRHKSWLKAEPPTIVVATVRSLCQMLEKQIFKLESMRVLVIDEVDFMFNSSKQVGSLRKLLTSYSSTNNRQTVFASASIPQHRRFLHDCIQQKWTKSDVVHVHVNPIEPMPSSLHHRFVICNKYERRQTLLSLLHSDAPECGIIFVGEQSEKSKKAGNAPSATLLVDFLKTSYGGPDILLLEEDMNFNLRAASLSEVRQRGGYLLISTDIAARGVDLPETTHIYNFDLPRTVVDYLHRAGRAARKPFSDQKCCVTNIITSEERFVLQRYENELMFHCEELFLQTQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A3X7</accession>
    <name>A0AA39A3X7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">GTP-binding protein OBGC, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_005764</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A3X7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009658">
        <property type="term" value="P:chloroplast organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01898">
        <property type="entry name" value="Obg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.210.12:FF:000001">
        <property type="entry name" value="GTPase Obg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000515">
        <property type="entry name" value="GTPase Obg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.350">
        <property type="entry name" value="GTP-binding protein OBG, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.210.12">
        <property type="entry name" value="GTP1/OBG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01454">
        <property type="entry name" value="GTPase_Obg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014100">
        <property type="entry name" value="GTP-bd_Obg/CgtA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036346">
        <property type="entry name" value="GTP-bd_prot_GTP1/OBG_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006074">
        <property type="entry name" value="GTP1-OBG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006169">
        <property type="entry name" value="GTP1_OBG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036726">
        <property type="entry name" value="GTP1_OBG_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045086">
        <property type="entry name" value="OBG_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015349">
        <property type="entry name" value="OCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02729">
        <property type="entry name" value="Obg_CgtA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03595">
        <property type="entry name" value="Obg_CgtA_exten"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008954">
        <property type="entry name" value="PRK12296.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008955">
        <property type="entry name" value="PRK12297.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008956">
        <property type="entry name" value="PRK12299.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11702">
        <property type="entry name" value="DEVELOPMENTALLY REGULATED GTP-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11702:SF44">
        <property type="entry name" value="GTP-BINDING PROTEIN OBGC, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09269">
        <property type="entry name" value="DUF1967"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01018">
        <property type="entry name" value="GTP1_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102741">
        <property type="entry name" value="Obg GTP-binding protein C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82051">
        <property type="entry name" value="Obg GTP-binding protein N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00905">
        <property type="entry name" value="GTP1_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51883">
        <property type="entry name" value="OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51881">
        <property type="entry name" value="OCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Obg" evidence="10">
        <location>
            <begin position="172"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="OBG-type G" evidence="8">
        <location>
            <begin position="335"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="domain" description="OCT" evidence="9">
        <location>
            <begin position="528"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="293"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="608"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="18"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9700085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="631" mass="69428" checksum="F447B2361E591D22" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A478</accession>
    <name>A0AA39A478_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Pyruvate, phosphate dikinase</fullName>
            <ecNumber evidence="3 12">2.7.9.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PVL29_005812</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>pyruvate + phosphate + ATP = phosphoenolpyruvate + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10756"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.9.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12 15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the PEP-utilizing enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.9.1" evidence="3 12"/>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A478"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050242">
        <property type="term" value="F:pyruvate, phosphate dikinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015979">
        <property type="term" value="P:photosynthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.60:FF:000040">
        <property type="entry name" value="Pyruvate, phosphate dikinase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000038">
        <property type="entry name" value="Pyruvate, phosphate dikinase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.10:FF:000009">
        <property type="entry name" value="Pyruvate, phosphate dikinase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.10">
        <property type="entry name" value="Phosphohistidine domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.189.10">
        <property type="entry name" value="Pyruvate Phosphate Dikinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008279">
        <property type="entry name" value="PEP-util_enz_mobile_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018274">
        <property type="entry name" value="PEP_util_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000121">
        <property type="entry name" value="PEP_util_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023151">
        <property type="entry name" value="PEP_util_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036637">
        <property type="entry name" value="Phosphohistidine_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002192">
        <property type="entry name" value="PPDK_AMP/ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010121">
        <property type="entry name" value="Pyruvate_phosphate_dikinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004531">
        <property type="entry name" value="PRK05878.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01828">
        <property type="entry name" value="pyru_phos_dikin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22931">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE DIKINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22931:SF9">
        <property type="entry name" value="PYRUVATE, PHOSPHATE DIKINASE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00391">
        <property type="entry name" value="PEP-utilizers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02896">
        <property type="entry name" value="PEP-utilizers_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01326">
        <property type="entry name" value="PPDK_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000853">
        <property type="entry name" value="PPDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52009">
        <property type="entry name" value="Phosphohistidine domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00742">
        <property type="entry name" value="PEP_ENZYMES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00370">
        <property type="entry name" value="PEP_ENZYMES_PHOS_SITE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate phosphate dikinase AMP/ATP-binding" evidence="17">
        <location>
            <begin position="143"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate phosphate dikinase AMP/ATP-binding" evidence="17">
        <location>
            <begin position="386"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="PEP-utilising enzyme mobile" evidence="16">
        <location>
            <begin position="507"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="domain" description="PEP-utilising enzyme C-terminal" evidence="18">
        <location>
            <begin position="603"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="13">
        <location>
            <position position="540"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="13">
        <location>
            <position position="918"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="646"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="703"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="832"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="832"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="853"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="854"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="855"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="856"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="856"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR000853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000853-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000853-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000853-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF01326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ9700167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="958" mass="104424" checksum="4128DA36887F7133" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A4G7</accession>
    <name>A0AA39A4G7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_006225</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A4G7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04051">
        <property type="entry name" value="C2_SRC2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044750">
        <property type="entry name" value="C2_SRC2/BAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32246:SF143">
        <property type="entry name" value="CALCIUM-DEPENDENT LIPID-BINDING (CALB DOMAIN) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32246">
        <property type="entry name" value="INGRESSION PROTEIN FIC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="2">
        <location>
            <begin position="71"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="288"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="378"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="567"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="577"/>
            <end position="596"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9700803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="596" mass="64357" checksum="E190965D59D6E0AF" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A660</accession>
    <name>A0AA39A660_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">NB-ARC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_006804</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A660"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF955">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0611">Plant defense</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NB-ARC" evidence="4">
        <location>
            <begin position="379"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="5">
        <location>
            <begin position="620"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="6">
        <location>
            <begin position="782"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="348"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9701581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1094" mass="125329" checksum="7BBF29BD40ABB901" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A6M3</accession>
    <name>A0AA39A6M3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Early nodulin-93</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_003904</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9701882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A6M3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005050">
        <property type="entry name" value="Enod93"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33605">
        <property type="entry name" value="EARLY NODULIN-93"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33605:SF3">
        <property type="entry name" value="EARLY NODULIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03386">
        <property type="entry name" value="ENOD93"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9701882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="110" mass="11694" checksum="2BD0836650B28C82" modified="2024-01-24" version="1">MGFGSETGDISTPRKTGFSITSIAESRKIVRAECVQEGVREATKAAGVAAVISTVATLGAVRYVPWVKQHINYTGRALIISAASVAAFFITADKTILECGKRNTTYDKPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A7B4</accession>
    <name>A0AA39A7B4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">tRNA (guanine-N(7)-)-methyltransferase</fullName>
            <ecNumber evidence="9">2.1.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">tRNA (guanine(46)-N(7))-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">tRNA(m7G46)-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_004110</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>guanosine(46) in tRNA + S-adenosyl-L-methionine = N(7)-methylguanosine(46) in tRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42708"/>
            <dbReference type="Rhea" id="RHEA-COMP:10188"/>
            <dbReference type="Rhea" id="RHEA-COMP:10189"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74480"/>
            <dbReference type="EC" id="2.1.1.33"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="9">tRNA modification; N(7)-methylguanine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.33" evidence="9"/>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A7B4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043527">
        <property type="term" value="C:tRNA methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008176">
        <property type="term" value="F:tRNA (guanine(46)-N7)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000158">
        <property type="entry name" value="tRNA (guanine-N(7)-)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03055">
        <property type="entry name" value="tRNA_methyltr_TrmB_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025763">
        <property type="entry name" value="Trm8_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003358">
        <property type="entry name" value="tRNA_(Gua-N-7)_MeTrfase_Trmb"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00091">
        <property type="entry name" value="tRNA (guanosine(46)-N7)-methyltransferase TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23417">
        <property type="entry name" value="3-DEOXY-D-MANNO-OCTULOSONIC-ACID TRANSFERASE/TRNA GUANINE-N 7 - -METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23417:SF16">
        <property type="entry name" value="TRNA (GUANINE-N(7)-)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02390">
        <property type="entry name" value="Methyltransf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51625">
        <property type="entry name" value="SAM_MT_TRMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 9" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="2 9" id="KW-0820">tRNA-binding</keyword>
    <feature type="active site" evidence="9">
        <location>
            <position position="151"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="95"/>
            <end position="96"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="128"/>
            <end position="129"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="226"/>
            <end position="228"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9702211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="251" mass="28388" checksum="D3C4A6A412A90299" modified="2024-01-24" version="1">MLKEGNSKISKSTHLPRKRFFRARAHSNPLSDSHFPVPISPSHVDYSVHFPQLFSSSDEGGDSKKIEFADVGCGFGGLLISLSTLFPQTLMIGMEIRDKVTEYVKERILALRVANPGQYQNVSVVRTNSMKYIPNYFGKGQLSKMFFLFPDPHFKEKNHRRRVISTHLLDEYAYVLGIGGIIYTITDVEELGDWMNECLEGHPMFEAITEEELEADPVVKLLSSATEEGQKVARNEGQTFQAVYRRIAPSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A9M7</accession>
    <name>A0AA39A9M7_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_004997</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A9M7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614">
        <property type="entry name" value="HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614:SF132">
        <property type="entry name" value="PROTEIN-LYSINE METHYLTRANSFERASE C42C1.13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10294">
        <property type="entry name" value="Methyltransf_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9703456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="270" mass="30073" checksum="FD812AF3FE4A2AF0" modified="2024-01-24" version="1">MVAGESDEDETDPMTVLLPDEPEVEKRLTSADEDAQKQRLQDNHIHSIDTTVVIRQLPSQGLSFQLWPAATTLVTLLDQLRSHPTNSPLSPILSALSNGQDHRPLHILELGSGTGLVGIVAAATLSANVTVTDLPHVIPNLQFNAEMNSHIWGPHGGTVEVAPLRWGEADDVELIGREFDLILASDVVYHDHLYDPLLQTLRILMGLEGADNKKMMFVMAHLRRWKKDSAFFRKAKKHFQVETLHVDRPTQGSRVGVITYSFRGKSQNFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AA03</accession>
    <name>A0AA39AA03_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_005161</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AA03"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000695">
        <property type="entry name" value="MYB transcription factor MYB48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000001">
        <property type="entry name" value="MYB-related transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015495">
        <property type="entry name" value="Myb_TF_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47994">
        <property type="entry name" value="F14D16.11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47994:SF5">
        <property type="entry name" value="F14D16.11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="HTH myb-type" evidence="6">
        <location>
            <begin position="9"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="5">
        <location>
            <begin position="9"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="6">
        <location>
            <begin position="62"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="5">
        <location>
            <begin position="62"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9703755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="242" mass="27212" checksum="F693B44C7743E72A" modified="2024-01-24" version="1">MGRSPCCEKAHTNKGAWTKEEDDRLIAHIQAHGEGCWRSLPKAAGLLRCGKSCRLRWINYLRPDLKRGNFTDEEDELIIKLHSLLGNKWSLIAGRLPGRTDNEIKNYWNTHIRRKLLSRGIDPLTHRPINEPAVDVATVSFGGVLKKEEVMSSGFGCKSRNPDEENCPDLNLELRISPPHHHPSPPVVDSRERGMLCFYCSLGLEKSKECSCSSGSGNGSSDDFLGMKNGVLDYRNLEMKRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ABG5</accession>
    <name>A0AA39ABG5_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_004954</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ABG5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004311">
        <property type="term" value="F:geranylgeranyl diphosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43281">
        <property type="entry name" value="FARNESYL DIPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43281:SF24">
        <property type="entry name" value="OS07G0580900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9703378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="73" mass="8260" checksum="68C972BCB1637602" modified="2024-01-24" version="1">MALDNVVPLRDPMGIRKAMRYTVLNGGKRNPLFCELVGGQESTVMPVACAMKMMYTMSLMQDDLLCMDNDDRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AC06</accession>
    <name>A0AA39AC06_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_003044</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat G protein beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AC06"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000580">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016346">
        <property type="entry name" value="G-protein_beta_1-5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001632">
        <property type="entry name" value="WD40_G-protein_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19850">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN BETA G PROTEIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25391">
        <property type="entry name" value="WD40_Gbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002394">
        <property type="entry name" value="GN-bd_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00319">
        <property type="entry name" value="GPROTEINB"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="61"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="152"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="200"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="244"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9704811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="381" mass="41355" checksum="F297F35E3554EEB3" modified="2024-01-24" version="1">MSVAELKERHMAATETVNSLRERLKQKRLLLLDTDVAGYARAQGKSPVTFGPTDLVCCRTLQGHTGKVYSLDWTTEKNRIVSASQDGRLIVWNALTSQKTHAIKLPCAWVMTCAFSPSGQSVACGGLDSVCSIFNLNSPTDKDGNLQISKMLSGHKGYVSSCQYVPDEDTHLITSSGDQTCVLWDITTGLRTSVFGGEFQSGHTADVLSVSISGSNSRMFVSGSCDATARLWDTRAASRAVRTFHGHEGDVNTVRFFPDGNRFGTGSDDGTCRLFDIRTGHQLQVYSQQHGDNEDPHVTSIAFSISGRLLFAGYSNGDCYVWDTLLAKVVLNLGSLQNSHDSRISCLGLSADGSALCTGSWDTNLKVSSLSFTFIYTMLLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ACA0</accession>
    <name>A0AA39ACA0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dirigent protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_002794</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Secreted</location>
            <location evidence="4">Extracellular space</location>
            <location evidence="4">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the plant dirigent protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ACA0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009699">
        <property type="term" value="P:phenylpropanoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.480.10">
        <property type="entry name" value="Allene oxide cyclase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044859">
        <property type="entry name" value="Allene_oxi_cyc_Dirigent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004265">
        <property type="entry name" value="Dirigent"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21495">
        <property type="entry name" value="NUCLEOPORIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03018">
        <property type="entry name" value="Dirigent"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0052">Apoplast</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041482066" description="Dirigent protein" evidence="4">
        <location>
            <begin position="25"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9704390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="190" mass="20860" checksum="A64107A728AEA5D8" modified="2024-01-24" version="1">MEKTALVLVAFSLAVAMAMPLVHSTIGDPKEVDEWLHKIRLAKQKMTRFHFYYHDIQNVSSMKVAQASITDKSPTLFGLTNMMDDPLTEGPELTSKQVGRAQGLYGLSGLNEFSLLVVMNLAFTSGEYNRSTLTILGRYESAQLTIQEMPIVGGSGVFRLAHGIASGRTYSRNATSGNAVVEFNIVAIHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AD36</accession>
    <name>A0AA39AD36_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PPPDE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_003068</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DeSI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AD36"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0101005">
        <property type="term" value="F:deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1720.30">
        <property type="entry name" value="PPPDE domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008580">
        <property type="entry name" value="PPPDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042266">
        <property type="entry name" value="PPPDE_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12378">
        <property type="entry name" value="DESUMOYLATING ISOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12378:SF83">
        <property type="entry name" value="PPPDE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05903">
        <property type="entry name" value="Peptidase_C97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01179">
        <property type="entry name" value="DUF862"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51858">
        <property type="entry name" value="PPPDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPPDE" evidence="4">
        <location>
            <begin position="74"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9704840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="337" mass="38319" checksum="9E4C414D23FC3368" modified="2024-01-24" version="1">MLLKTIPSHFNGCSLQQGWHRALISSRLQPRGNMKSGSKKQWNSIVPFRLRGKSATRFCMYPKVKSAGRSPGNTPVYLNVYDLTAINGYVYWAGLGVFHSGVEVHGVEFAFGAHDYPASGVFEVEPRQCPGFKFRKAIFMGTTCLDPGQFREFMERYSANYNGDTYHLIIKNCNHFCEDICYKLTGNRIPKWVNRLARIGSLCNCILPQALKASAVRHKPNFQNYEIEKKRLKTAFSCLSSISMHQKEVSLSSLFLHSHYKGCLPPWELKTSKNNLYCNVIDLDMLSYIFIAEEMDGCILLKPSLTYKGFYFLFFSGLNILCLCISNPANPVEEYSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ADJ0</accession>
    <name>A0AA39ADJ0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SWIM-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_003529</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ADJ0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06410">
        <property type="entry name" value="PB1_UP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018289">
        <property type="entry name" value="MULE_transposase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004332">
        <property type="entry name" value="Transposase_MuDR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006564">
        <property type="entry name" value="Znf_PMZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31973">
        <property type="entry name" value="POLYPROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31973:SF157">
        <property type="entry name" value="SWIM-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03108">
        <property type="entry name" value="DBD_Tnp_Mut"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10551">
        <property type="entry name" value="MULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04434">
        <property type="entry name" value="SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00575">
        <property type="entry name" value="ZnF_PMZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="5">
        <location>
            <begin position="582"/>
            <end position="614"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9705527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="713" mass="81847" checksum="20D490B284E31E29" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AEE0</accession>
    <name>A0AA39AEE0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ABC-type xenobiotic transporter</fullName>
            <ecNumber evidence="3">7.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_001590</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O + xenobioticSide 1 = ADP + phosphate + xenobioticSide 2.</text>
            <dbReference type="EC" id="7.6.2.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.2" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008559">
        <property type="term" value="F:ABC-type xenobiotic transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18579">
        <property type="entry name" value="ABC_6TM_ABCC_D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18580">
        <property type="entry name" value="ABC_6TM_ABCC_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000003">
        <property type="entry name" value="ABC transporter C family member 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000923">
        <property type="entry name" value="ABC transporter C family member 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000169">
        <property type="entry name" value="ABC transporter C family member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000002">
        <property type="entry name" value="ABC transporter C family member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056228">
        <property type="entry name" value="ABCC10-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044746">
        <property type="entry name" value="ABCC_6TM_D1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044726">
        <property type="entry name" value="ABCC_6TM_D2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF263">
        <property type="entry name" value="ABC-TYPE XENOBIOTIC TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24358">
        <property type="entry name" value="ABCC10_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="33"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="86"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="121"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="153"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="186"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="322"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="454"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="544"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="930"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="966"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1058"/>
            <end position="1082"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1103"/>
            <end position="1122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1152"/>
            <end position="1174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1181"/>
            <end position="1202"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="15">
        <location>
            <begin position="323"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="14">
        <location>
            <begin position="640"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="15">
        <location>
            <begin position="941"/>
            <end position="1210"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="14">
        <location>
            <begin position="1247"/>
            <end position="1481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9706095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1498" mass="166899" checksum="12E26A4F07DDA28F" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AEK1</accession>
    <name>A0AA39AEK1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_003092</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Cold-regulated 413 protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AEK1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008892">
        <property type="entry name" value="COR413"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33596:SF1">
        <property type="entry name" value="COLD-REGULATED 413 PLASMA MEMBRANE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33596">
        <property type="entry name" value="COLD-REGULATED 413 PLASMA MEMBRANE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05562">
        <property type="entry name" value="WCOR413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="120"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="144"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="180"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9704879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="202" mass="22519" checksum="8786798614A45F2D" modified="2024-01-24" version="1">MGKKGYLAMRTDTDTTDLISSDLRDLGNAAKKLATHAIKLGSLGFGTTFLEWVASFAAIYLLILDRTNWKTNILTALLIPYIFFSLPSILFNFFSGQVGKWIAFIAVVLRLFFPKRFPDWLEMPAALILLIVVAPSLFSSTIRGDWIGLVICLAIGCYLLQEHIRASGGFRNSFTQRHGISNTIGLIVLLVYPVWALVLDFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AFL6</accession>
    <name>A0AA39AFL6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF7963 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_001713</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AFL6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058269">
        <property type="entry name" value="DUF7963"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32166:SF24">
        <property type="entry name" value="F16P17.2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32166">
        <property type="entry name" value="OSJNBA0013A04.12 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25908">
        <property type="entry name" value="DUF7963"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7963" evidence="1">
        <location>
            <begin position="8"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9706324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="681" mass="77835" checksum="9CA03155E35A5D7E" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AGC3</accession>
    <name>A0AA39AGC3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Haloacid dehalogenase-like hydrolase domain-containing protein Sgpp</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_002213</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AGC3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07505">
        <property type="entry name" value="HAD_BPGM-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="entry name" value="Putative phosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051600">
        <property type="entry name" value="Beta-PGM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46193">
        <property type="entry name" value="6-PHOSPHOGLUCONATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46193:SF9">
        <property type="entry name" value="HALOACID DEHALOGENASE-LIKE HYDROLASE DOMAIN-CONTAINING PROTEIN SGPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9707121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="252" mass="27754" checksum="289439FCE22419DA" modified="2024-01-24" version="1">MPKTISPSKSVKYSLSGLAPLEAVLFDIDGTLCDSDPLHYYAFRELLLQIGYNGGVPITEEFFIEKVAGKHNDDIARGLFPDWDLEKGLKFTDDKEALFRKLAKDQVKPLNGLHKIRKWIEDRGLKRAAVSNAPRPNAELMISLLGLSDFFHAVVVGSECDRAKPFPDPYLKALEVLQVSKDHTFVFEDSVSGIKAGVAAEMPVVGLTTRNPESLLMEAKPVFLIRDYDDPKLWAALAELDQKGAPGATAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AGC7</accession>
    <name>A0AA39AGC7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein downstream neighbor of Son</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_003836</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DONSON family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AGC7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033260">
        <property type="term" value="P:nuclear DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024861">
        <property type="entry name" value="Donson"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12972">
        <property type="entry name" value="DOWNSTREAM NEIGHBOR OF SON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12972:SF0">
        <property type="entry name" value="PROTEIN DOWNSTREAM NEIGHBOR OF SON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02064">
        <property type="entry name" value="DONSON"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041435545" description="Protein downstream neighbor of Son" evidence="5">
        <location>
            <begin position="32"/>
            <end position="664"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9705913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="664" mass="72167" checksum="7E94545E88A8C303" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ALC5</accession>
    <name>A0AA39ALC5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transposase-associated domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_000502</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ALC5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029480">
        <property type="entry name" value="Transpos_assoc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13963">
        <property type="entry name" value="Transpos_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase-associated" evidence="2">
        <location>
            <begin position="3"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9708493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="140" mass="16263" checksum="F498BF21D8863347" modified="2024-01-24" version="1">MDKSWMNLRDRLCKEYVNGIRTFIQVAKNHVNKEGKSRCPCRDCQNAFWKSINDIEIHLYRFGIATTYQRWIFHGEKVDINYYKPVDLNSGIDKVDHLDDDDDDGDDDNNDDLVDTSQSRYVPMGNIQEGSKTGGRQSSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D4D8</accession>
    <name>A0AA39D4D8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">UDP-glycosyltransferases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_025320</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D4D8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035251">
        <property type="term" value="F:UDP-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000037">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050481">
        <property type="entry name" value="UDP-glycosyltransf_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48049">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48049:SF91">
        <property type="entry name" value="UDP-GLYCOSYLTRANSFERASE 79B7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9671558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="363" mass="40109" checksum="C11CBD17F8F5A504" modified="2024-01-24" version="1">MDRTTDQVEAALRALKPGFLLFDFPYWAPALASKLGIKSIYYSAVCAAALARHPVPGGQVSKDRPIAAVPPPGYPSLTVVLRPHEAWMEQLPYAPFGEGVNLYQRLTTGMKCCDAISIRTCHEIEGPFCDYLASQYGKPVLLTGPVLPKPLPTPSEDRWEQWLSGFKPGSVIFCAFGSQHFPEKDQFQELLLGLELTGLPFLVALKPPTGAATIEEALPEGFQERVGGRGVVHGGWVPQPSILSHPSVGCFVSHCGFGSMWESLTSDPQIVLVPELLDQILNTRLLAEVLKVAVEIEKEENGWFLKESLCRAVRSVMDEESEVGGLVRKNHAKWKETLTSQGFMSNYIDNFVHQLQQLLVKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D5X3</accession>
    <name>A0AA39D5X3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_026421</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9673133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D5X3"/>
    <dbReference type="SMR" id="A0AA39D5X3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36338">
        <property type="entry name" value="OS02G0495900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36338:SF1">
        <property type="entry name" value="OS02G0495900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9673133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="127" mass="14796" checksum="92932DCD385F0AF2" modified="2024-01-24" version="1">MSVLWEKSETWRWIVRKSRDSKPFFLAFATICGVVPGVIGYCVMQFTNSRNPELEAQLRSNARPDSLMMGKVNQERLAEFLGELQRKEDTNDRYVAALRGETLTRKPYERIQPIPKQSNMEAKEQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D6Q6</accession>
    <name>A0AA39D6Q6_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_025650</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D6Q6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1720.10">
        <property type="entry name" value="endopeptidase domain like (from Nostoc punctiforme)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31354">
        <property type="entry name" value="OS01G0793500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31354:SF2">
        <property type="entry name" value="OS01G0793500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041467992" evidence="1">
        <location>
            <begin position="34"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9672085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="524" mass="59463" checksum="CF92FCF0B8C874D2" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D7P7</accession>
    <name>A0AA39D7P7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phase-change related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_025754</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D7P7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010800">
        <property type="entry name" value="GRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37389:SF16">
        <property type="entry name" value="GLYCINE-RICH CELL WALL STRUCTURAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37389">
        <property type="entry name" value="NODULIN-24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07172">
        <property type="entry name" value="GRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041329976" description="Phase-change related protein" evidence="2">
        <location>
            <begin position="27"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9672262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="88" mass="9335" checksum="AEA40D972E20E716" modified="2024-01-24" version="1">MAYSKAFLLLGLLCAVVLVLSSEVSARELAEAAQTQGSVEEAKHWGHGHGHGHWGHGHGHGHRHGHHGKPGHGGAAETQENESEAREN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D835</accession>
    <name>A0AA39D835_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">mitogen-activated protein kinase kinase kinase</fullName>
            <ecNumber evidence="2">2.7.11.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_023838</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.25" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D835"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004709">
        <property type="term" value="F:MAP kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000357">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050538">
        <property type="entry name" value="MAP_kinase_kinase_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016">
        <property type="entry name" value="MAP KINASE KINASE KINASE SSK2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016:SF12">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="355"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="332"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="95"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="333"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="384"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9674531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="716" mass="78795" checksum="FAA056A8C242001A" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D8W2</accession>
    <name>A0AA39D8W2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Tubulin beta chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_024103</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11 12">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="12">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the tubulin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D8W2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02187">
        <property type="entry name" value="beta_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000002">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000002">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000005">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013838">
        <property type="entry name" value="Beta-tubulin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002453">
        <property type="entry name" value="Beta_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01163">
        <property type="entry name" value="BETATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00228">
        <property type="entry name" value="TUBULIN_B_AUTOREG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9 12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 12" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="14">
        <location>
            <begin position="49"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="15">
        <location>
            <begin position="248"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="427"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="431"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9674994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="448" mass="50213" checksum="9DE34C130BE8F6F9" modified="2024-01-24" version="1">MREILHVQGGQCGNQIGSKFWEVICDEHGVDPTGRYNGDGSSDLQLERINVYYNEASGGRYVPRAVLMDLEPGTMDSIRSGPYGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELIDAVLDVVRKEAENCDCMQGFQVCHSLGGGTGSGMGTLLISKMREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLSTPSFGDLNHLISATMSGVTCCLRFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTSRGSQQYISLTVPELTQQMWDAKNMMCAADPRHGRYLTASAMFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDIPPRGLKMASTFVGNSTSIQEMFRRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATADDEGEYEDGAEENYEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D999</accession>
    <name>A0AA39D999_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Laccase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_024360</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the multicopper oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D999"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002355">
        <property type="entry name" value="Cu_oxidase_Cu_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF261">
        <property type="entry name" value="LACCASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00080">
        <property type="entry name" value="MULTICOPPER_OXIDASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0186">Copper</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Plastocyanin-like" evidence="9">
        <location>
            <begin position="17"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="10">
        <location>
            <begin position="220"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9675399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="394" mass="44675" checksum="20753D4A3388DAB4" modified="2024-01-24" version="1">MFYINQEHTIHVIFIFLSVDYGKTYLLRIVNVVVHAELFFAISQHNLTAVGTDATYTKPLVTDYIMIAPGQTMDVLVTANQTPSSCYMAARQFVTVDPTQAGYVNMKVTAILEYSGNYTPPSSPSFPANFPPYHQLDIAYGFVEQLRSLANTEHPVNVPLNITTRMFITASVNVVSYKFEGAEESHIATSLNNISFMDPSSSILDAYYRHISGVYYPDFPDYPPSYYNFTFIEEDLPSGITKPAMKVKVLNYNESVEIIFQGTNILLSVEDHPMHLHRYTFYVIGMGHRNFNPESDPKAFNLVDPPEQNTIVVRKNGWVALRFVANNPGMFFKILLLRLHCHLERHFTWGMATVFIVKDGGTPETSLLPPPDYMPPCIPPSSIHLEDFNDRWSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DAB9</accession>
    <name>A0AA39DAB9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">PVL29_022016</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="25">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="25">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9676796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DAB9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000309">
        <property type="entry name" value="Leucine-rich repeat receptor protein kinase MSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000177">
        <property type="entry name" value="Leucine-rich repeat receptor-like serine/threonine-protein kinase At1g17230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000356">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000445">
        <property type="entry name" value="MDIS1-interacting receptor like kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000400">
        <property type="entry name" value="Nuclear pore complex protein NUP107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000416">
        <property type="entry name" value="Probable leucine-rich repeat receptor-like protein kinase At5g63930"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051420">
        <property type="entry name" value="Ser_Thr_Kinases_DiverseReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48005">
        <property type="entry name" value="LEUCINE RICH REPEAT KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48005:SF95">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="16 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10 23" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 22" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="23">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041335520" description="non-specific serine/threonine protein kinase" evidence="23">
        <location>
            <begin position="25"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="704"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="24">
        <location>
            <begin position="769"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="798"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="KAJ9676796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1034" mass="113417" checksum="03FA48B6F0D8A051" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DAX7</accession>
    <name>A0AA39DAX7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein TIFY</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Jasmonate ZIM domain-containing protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_021998</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Repressor of jasmonate responses.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="2">The jas domain is required for interaction with COI1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the TIFY/JAZ family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9676765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DAX7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031347">
        <property type="term" value="P:regulation of defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000022">
        <property type="term" value="P:regulation of jasmonic acid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009611">
        <property type="term" value="P:response to wounding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018467">
        <property type="entry name" value="CCT_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040390">
        <property type="entry name" value="TIFY/JAZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010399">
        <property type="entry name" value="Tify_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33077">
        <property type="entry name" value="PROTEIN TIFY 4A-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33077:SF90">
        <property type="entry name" value="PROTEIN TIFY 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09425">
        <property type="entry name" value="Jas_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06200">
        <property type="entry name" value="tify"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00979">
        <property type="entry name" value="TIFY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51320">
        <property type="entry name" value="TIFY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1184">Jasmonic acid signaling pathway</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tify" evidence="4">
        <location>
            <begin position="198"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="262"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="307"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU369065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9676765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="365" mass="38090" checksum="0CE1C9CA381446F0" modified="2024-01-24" version="1">MERDFLGLGSGKVLPSGKEESSDSVPARGLGMQWSFPNKVSAIPHFLSFKAVQEDRPRRGVYDHPFASSGLMPVSTADALESNRQPHPGVVQKNLNLEKQVGSQFVMTGYPLQHLDARSVRRAHEVGKFPASNQPNHSISVAFSNPVLQSQIAFSGPSVGGSAVKPQPLGGVPVAGPVSFPTICSFIGSTDQKKASKPPGPATQLTIFYAGSVNVFDGISPEQAQAIMLLAGNVPPVSPNTTLPAPQGQGQIIMPSGGDGFIRNQPHTSQPGSGLPTPISAPSNVGSQPGGGPNSIGELMAGKTKGALTSTNQPEPPNVVNSLGSTTPTYIPAVPQARKASLARFLEKRKERMMSASPYHGSTPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DB35</accession>
    <name>A0AA39DB35_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_024634</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DB35"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9675787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="218" mass="23743" checksum="653DFA935539A922" modified="2024-01-24" version="1">MCFQNFLKASACSQLTCLLGSPFHWTRYSITGGTPCLLVTRSARIFFTFFSREASDGCRPVRFRCLECCFLLPIEFTFPLVTSSMCVRATSARSPFAPFTCTPDNKEVLGVLGFVLNWRAPSSCIEPICASSSCSLSSIMALSAFLFGQSRAQCGPSHRKHLILGVNSFLSALPLPSRTLGVLPDPFLGALWSLGTSSPPPMAWLSSKDSTSEEFPLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DCB0</accession>
    <name>A0AA39DCB0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_020494</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DCB0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016709">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11075">
        <property type="entry name" value="CYP77_89"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000012">
        <property type="entry name" value="Cytochrome P450 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051103">
        <property type="entry name" value="Plant_metabolite_P450s"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24298:SF800">
        <property type="entry name" value="CYTOCHROME P450 89A2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24298">
        <property type="entry name" value="FLAVONOID 3'-MONOOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12 13" id="KW-0349">Heme</keyword>
    <keyword evidence="9 12 13" id="KW-0408">Iron</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 13" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="8 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="12">
        <location>
            <position position="473"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9678340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="519" mass="59446" checksum="E0AB3AF164EA444F" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DCM6</accession>
    <name>A0AA39DCM6_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_020914</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DCM6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9678864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="190" mass="21491" checksum="139C8F3B789B32DA" modified="2024-01-24" version="1">MSMAIVVMPMKVTCVPFNGNGGVHHHDPRDLEGPNRHHELLRKVHDVSRYDSCRAHDVVYRRVRVVLLHDGCDALLHDGHDGLRVHDVLHLHGRQEHLEEEDGDGLGEVSWRGRSDPHEYVIGKTVPEHGGGVPRCDHSDHHVDGVLRHDHGVHGVYDSYEHKETDDDEAFSRQNLTRGSLDVVVASLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DD60</accession>
    <name>A0AA39DD60_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_019820</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DD60"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044715">
        <property type="entry name" value="WDR86-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44489">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44489:SF1">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="25"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="145"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="269"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="309"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="25"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="53"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="14"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="55"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="88"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9680618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="434" mass="47315" checksum="E67B2407FB0D1282" modified="2024-01-24" version="1">MDDKRVKVGGKAPVRTRGRGGYGKKKNGAVCMYWLQGRCNRNPCRFVHAAGEVNITPNPTNSRSYTYHRKNPTDSTSNSDSKQKKQKQQQQQSLSLTSGPSGDRTHASHISQQDSHPESHEESSLSTRGSSGDGIANLFMRLAQLDGHNKTVTGIALPSGSDKLYTASGDGDLRVWDCHTGRCVHQGNLGAQIGCLISQGPWLFAGLRNLVKAWNLKTETQYSIDGPVGQVYALEATTEDVLFAGMEDGNIFTWKYDPKTDSFPLITIFKGHTASVLSLQVGCKSLYSGSMDNTIRRWDLDTLTCSQTLLGHSAAVTSLVLSGDGCLLSCSLDQTIKIWACRQDGSMGVFDTRNEEHPILALFGMHDAHDKPILCCSCNDNSVILYELPSFTKRGTIFGKQEVGRIQSGLGGLFFTGDGTGQVAVWKWLTPRTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DES6</accession>
    <name>A0AA39DES6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_021476</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Required for integral membrane and secreted preprotein translocation across the endoplasmic reticulum membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Interacts with OEP61/TPR7.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DES6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031207">
        <property type="term" value="C:Sec62/Sec63 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006620">
        <property type="term" value="P:post-translational protein targeting to endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000044">
        <property type="entry name" value="DnaJ protein ERDJ2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000038">
        <property type="entry name" value="DnaJ protein ERDJ2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3380.10:FF:000007">
        <property type="entry name" value="DnaJ protein ERDJ2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.10">
        <property type="entry name" value="Sec63 N-terminal domain-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004179">
        <property type="entry name" value="Sec63-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24075">
        <property type="entry name" value="SEC63 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24075:SF0">
        <property type="entry name" value="TRANSLOCATION PROTEIN SEC63 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02889">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00973">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158702">
        <property type="entry name" value="Sec63 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="66"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="192"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="14">
        <location>
            <begin position="99"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="614"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="624"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="676"/>
            <end position="688"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00056231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00064009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9679567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="688" mass="76840" checksum="722DC82B6AD656F0" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DF87</accession>
    <name>A0AA39DF87_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_020408</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">May function as a signaling molecule by regulating the trafficking of other regulators.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MCTP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9681520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DF87"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd04022">
        <property type="entry name" value="C2A_MCTP_PRT_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08378">
        <property type="entry name" value="C2B_MCTP_PRT_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04019">
        <property type="entry name" value="C2C_MCTP_PRT_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08379">
        <property type="entry name" value="C2D_MCTP_PRT_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000323">
        <property type="entry name" value="C2 calcium/lipid-binding plant phosphoribosyltransferase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000090">
        <property type="entry name" value="C2 domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000119">
        <property type="entry name" value="C2 domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000128">
        <property type="entry name" value="C2 domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047257">
        <property type="entry name" value="C2B_MCTP_PRT_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047258">
        <property type="entry name" value="C2C_MCTP_PRT_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047255">
        <property type="entry name" value="C2D_MCTP_PRT_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013583">
        <property type="entry name" value="MCTP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047259">
        <property type="entry name" value="QUIRKY-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31425:SF32">
        <property type="entry name" value="MULTIPLE C2 DOMAIN AND TRANSMEMBRANE REGION PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31425">
        <property type="entry name" value="PHOSPHORIBOSYLANTHRANILATE TRANSFERASE ISOFORM 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08372">
        <property type="entry name" value="PRT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="736"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="820"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="850"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="965"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="10">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="10">
        <location>
            <begin position="262"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="10">
        <location>
            <begin position="426"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="10">
        <location>
            <begin position="595"/>
            <end position="720"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00053146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9681520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1022" mass="116444" checksum="8221A9F11AA6238F" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DFZ3</accession>
    <name>A0AA39DFZ3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glabrous enhancer-binding protein-like DBD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_018257</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GeBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DFZ3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007592">
        <property type="entry name" value="GEBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053932">
        <property type="entry name" value="GeBP-like_DBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31662">
        <property type="entry name" value="BNAANNG10740D PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31662:SF1">
        <property type="entry name" value="OS01G0249900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04504">
        <property type="entry name" value="GeBP-like_DBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glabrous enhancer-binding protein-like DBD" evidence="3">
        <location>
            <begin position="126"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="234"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="40"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="70"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="105"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="245"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="261"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9682285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="395" mass="43876" checksum="43DA4327A9215E92" modified="2024-01-24" version="1">MASAEDPTTVFGEEDLDDDEDEDSEEEEENLALPSHQDDDVLDEDDEDDEDDDVLDEASASPSTSGDPHVSSSSAAPVVTVPVVTVAVPGVPNGEPIPAAVDSASDPKRPRAGEEKKPFDESRRLFQRLWTDEDEIELLQGFLEYSAQRGPNSSSHHHDTTAFYDQIKSKLQLDFNKNQLVEKLRRLKKKYRNVVSRISSGKEFSFKSPHDQSTFEISRKIWSTTMAITGVPEETGLEDDDANPNPNPTTNPSPNPNPTTKGFNSYSIDVNSAEKKVPRSRKRSRAKLDEKPVLNNQNHEMPSSIPSLIEETVRSCLSPLFKELLHNAVNGPSCGARGFGGMALNPMPLSFGGTEVTDERWRKQQILELEVYSKRLELVQEQIRSALQELRGMGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DHX4</accession>
    <name>A0AA39DHX4_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_016858</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DHX4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9684594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="118" mass="13313" checksum="B0BDF5D65BD42963" modified="2024-01-24" version="1">MANSALPATCWMKCLVHKDTVTEKHVTERLIELEPEAAVSYLLLDNSYTDVEKCMPAKRIKDLLKDGIITMKPGLSWIEVEVKPCGKRVGVEKRRGRKGLSPQVNKWMGSEEDDGVDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DIT6</accession>
    <name>A0AA39DIT6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Germin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_015371</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May play a role in plant defense. Probably has no oxalate oxidase activity even if the active site is conserved.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Secreted</location>
            <location evidence="2 13">Extracellular space</location>
            <location evidence="2 13">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the germin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DIT6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02241">
        <property type="entry name" value="cupin_OxOx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000005">
        <property type="entry name" value="Germin-like protein subfamily 1 member 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006045">
        <property type="entry name" value="Cupin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001929">
        <property type="entry name" value="Germin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019780">
        <property type="entry name" value="Germin_Mn-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31238">
        <property type="entry name" value="GERMIN-LIKE PROTEIN SUBFAMILY 3 MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00190">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00325">
        <property type="entry name" value="GERMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00835">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00725">
        <property type="entry name" value="GERMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 13" id="KW-0052">Apoplast</keyword>
    <keyword evidence="7 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 10 13" id="KW-0464">Manganese</keyword>
    <keyword evidence="6 10 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0964">Secreted</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041485595" description="Germin-like protein" evidence="13">
        <location>
            <begin position="21"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="Cupin type-1" evidence="14">
        <location>
            <begin position="59"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="30"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU366015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9686438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="215" mass="22837" checksum="B6B1927FDD74A4DB" modified="2024-01-24" version="1">MAKHFTLLSLLALTCCIAFAFEPKPLQDFCVADPNSSARVNGLVCKNPMLAQANDFSFSGLHVAGNTSNPFGSRVTPVTVAQLPGLNTLGISMARIDYAPWGINPPHAHPRASEILTVLEGPILVGFVTSNPENRLITKVLQKGDVFVFPVGLIHFQRNVGYGNAVAIAALSSQNPGVITIANAVFGSNAPIASEVLAKAFQVDKNIVDQLQSKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DN83</accession>
    <name>A0AA39DN83_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_013160</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DN83"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.330:FF:000001">
        <property type="entry name" value="Protein SRG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050295">
        <property type="entry name" value="Plant_2OG-oxidoreductases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47991">
        <property type="entry name" value="OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="6">
        <location>
            <begin position="209"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9690866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="366" mass="41171" checksum="A52EEF36CBC710BC" modified="2024-01-24" version="1">MDAQKLTSLGGSLLVPSVQELAKKTLTTVPPRYLRPEQDPPFLSDSSTTCSSIHVPVIDIHRLVSGDVLDSELDKLHNACKDWGFFQLTNHGVSSTLVEKVKVEVHEFFNLPMEEKKKFWQQPEDLEGFGQAFVLSEEQKLDWADMFYMITLPTYLRKPHLFPKLPISLRDSLEVYAVELRNLAMTILGFIAKALKMEANDMKELFEEGHQGMRMNYYPPCPQPDQVIGLTPHSDPVGLTILLQVNEMEGLQIRKDGMWVPIKPLPGAFIVNIGDILEIVTNGAYRSIEHRATVNSIKERLSIATFYSAKLNGDMGPAPSLVSPDSPALFKRIGVSDYFKGLFSRELYGKSYLEVLRSETGEAQNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DQ80</accession>
    <name>A0AA39DQ80_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Receptor-like serine/threonine-protein kinase</fullName>
            <ecNumber evidence="14">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PVL29_012736</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="14"/>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DQ80"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048544">
        <property type="term" value="P:recognition of pollen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00028">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01098">
        <property type="entry name" value="PAN_AP_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.90.10.10:FF:000001">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000467">
        <property type="entry name" value="Liguleless narrow1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001037">
        <property type="entry name" value="Putative G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.10">
        <property type="entry name" value="Bulb-type lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021820">
        <property type="entry name" value="S-locus_recpt_kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000858">
        <property type="entry name" value="S_locus_glycoprot_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024171">
        <property type="entry name" value="SRK-like_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002:SF1082">
        <property type="entry name" value="OS06G0693000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002">
        <property type="entry name" value="RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD1-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11883">
        <property type="entry name" value="DUF3403"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08276">
        <property type="entry name" value="PAN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00954">
        <property type="entry name" value="S_locus_glycop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000641">
        <property type="entry name" value="SRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00473">
        <property type="entry name" value="PAN_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="4 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="478"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="18">
        <location>
            <begin position="71"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="19">
        <location>
            <begin position="383"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="562"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="590"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR000641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAJ9690232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="880" mass="99529" checksum="A9F5A3BF30FF5EEE" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DSN6</accession>
    <name>A0AA39DSN6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Cation/H(+) antiporter 20</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_010241</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the monovalent cation:proton antiporter 2 (CPA2) transporter (TC 2.A.37) family. CHX (TC 2.A.37.4) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DSN6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006885">
        <property type="term" value="P:regulation of pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1530.20:FF:000003">
        <property type="entry name" value="Cation/H(+) antiporter 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057291">
        <property type="entry name" value="CHX17_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057290">
        <property type="entry name" value="CHX17_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050794">
        <property type="entry name" value="CPA2_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32468">
        <property type="entry name" value="CATION/H + ANTIPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32468:SF98">
        <property type="entry name" value="CATION_H+ EXCHANGER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23256">
        <property type="entry name" value="CHX17_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23259">
        <property type="entry name" value="CHX17_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0050">Antiport</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0630">Potassium</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="55"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="91"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="120"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="153"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="190"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="223"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="263"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="341"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="406"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="12">
        <location>
            <begin position="42"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H(+) antiporter central" evidence="13">
        <location>
            <begin position="487"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H(+) antiporter C-terminal" evidence="14">
        <location>
            <begin position="626"/>
            <end position="796"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF23256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF23259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9694674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="825" mass="89376" checksum="17FFD13B4C76F570" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DU84</accession>
    <name>A0AA39DU84_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Acyl-activating enzyme 18, peroxisomal</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_007065</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9697766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DU84"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44378">
        <property type="entry name" value="ACYL-ACTIVATING ENZYME 17, PEROXISOMAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44378:SF1">
        <property type="entry name" value="ACYL-ACTIVATING ENZYME 18, PEROXISOMAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="242"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="2">
        <location>
            <begin position="189"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="3">
        <location>
            <begin position="642"/>
            <end position="707"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9697766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="727" mass="80528" checksum="70671FCF09CE8546" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DVS2</accession>
    <name>A0AA39DVS2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DYW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_008188</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. PCMP-H subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9699476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DVS2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000366">
        <property type="entry name" value="Pentatricopeptide (PPR) repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000382">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000196">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At4g14850"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000073">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000031">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000201">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032867">
        <property type="entry name" value="DYW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046849">
        <property type="entry name" value="E2_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24015:SF1856">
        <property type="entry name" value="OS05G0455900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24015">
        <property type="entry name" value="OS07G0578800 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14432">
        <property type="entry name" value="DYW_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20430">
        <property type="entry name" value="Eplus_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="157"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="258"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="360"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="457"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="559"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="domain" description="DYW" evidence="4">
        <location>
            <begin position="774"/>
            <end position="866"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9699476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="866" mass="96051" checksum="11B72D9C58B22B71" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DYQ9</accession>
    <name>A0AA39DYQ9_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_004716</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DYQ9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000213">
        <property type="entry name" value="Tyrosine-sulfated glycopeptide receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051502">
        <property type="entry name" value="RLP_Defense_Trigger"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48062:SF21">
        <property type="entry name" value="RECEPTOR-LIKE PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48062">
        <property type="entry name" value="RECEPTOR-LIKE PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9703061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="754" mass="85253" checksum="AC1DF38704573E88" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DYZ9</accession>
    <name>A0AA39DYZ9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_007917</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9699067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DYZ9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044653">
        <property type="entry name" value="AZF1/2/3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45988">
        <property type="entry name" value="C2H2 TYPE ZINC FINGER TRANSCRIPTION FACTOR FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45988:SF18">
        <property type="entry name" value="C2H2-TYPE ZINC FINGER FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="113"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="387"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="78"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="173"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="395"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="173"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="395"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9699067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="445" mass="48397" checksum="E5D698C6EDA4E8C2" modified="2024-01-24" version="1">MGEAVPCDGEERQGLGSAGLPENRLSLKPINIPMGGGDEVDDGKSSKVIVEARLECNIRSKGLGCSEGFKGRMRARLDDQGDRVSRSNKNKTWNLKNGSEGGVAENIGGTDQAKCPVCSKSFHSKKAMYGHMRCHPEREWRGINPPPSTKTASCSSVSQEIDGLSHASMTSTVVSSDISPSQPPLQWTKTGRRRQNRIINSYSSSGSSCNISSSQNKGIEEKHQVSECAGLQKRQQLKMLKLGNLRNYPSKKLKIHERGALGFRGQEDAGNKSGASWKDFLKIGFNLGPKEVDTDKKSDGDGIIPGGQLDDQMDVSDNKRFLKTLLPRKYKCGTCNKTGTCNKTFPTYQSLTMGNKYASSSHTAASEEEGQALGTSKHAKQLVQKSHKCRTCNKSFPTGQSLGGHQTTHRQKPAQLAAPKQEASQSAGPRVLDFDLNELPPMEKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DZ94</accession>
    <name>A0AA39DZ94_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_008101</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9699337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DZ94"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15539">
        <property type="entry name" value="PHD1_AIRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000494">
        <property type="entry name" value="Acyl-CoA N-acyltransferase with RING/FYVE/PHD-type zinc finger domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000506">
        <property type="entry name" value="Acyl-CoA N-acyltransferase with RING/FYVE/PHD-type zinc finger domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056511">
        <property type="entry name" value="IDM1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032308">
        <property type="entry name" value="TDBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47025">
        <property type="entry name" value="AUTOIMMUNE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47025:SF2">
        <property type="entry name" value="AUTOIMMUNE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23209">
        <property type="entry name" value="IDM1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16135">
        <property type="entry name" value="TDBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="8">
        <location>
            <begin position="497"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="9">
        <location>
            <begin position="694"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="31"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="358"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="56"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="74"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="134"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="148"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="184"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="378"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9699337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="856" mass="93782" checksum="BBDCA013DD8CEF98" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E0W1</accession>
    <name>A0AA39E0W1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycosyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_004808</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="5"/>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E0W1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035251">
        <property type="term" value="F:UDP-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000020">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000095">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050481">
        <property type="entry name" value="UDP-glycosyltransf_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48048">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48048:SF30">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9703179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="477" mass="52149" checksum="F565513FBDF187CD" modified="2024-01-24" version="1">MESVVLYPSPGMGHLISMVELGKLILKHHPSFSITIFIVTPPYNTGSTAPYLARVSSAIPSITFHHLPTISLPLDSFSSPNHETLAFELLRLNNPNVHQALVSISNNSSVRALIVDGFCTAALSVAAQLNIPCYYFFTSGACCLASFLYLPFIHQQTTKSFKDLNIHLHIPGLPPIPASDMAKPILDREDKAYEFFLNRSIHLPRSAGIIVNNFEALEPRAIKTILDGLCVLDGPTPPIFCTGPLIAADDRSGGGGGGGGGIPECLTWLESQPKRSVLFLCFGSLGLFSEEQLKEVAVGLERNGQRFLWVVRSPPSKDPSRRFLAPPEPDLNSLLPDGFLDRTKERGLVVKSWAPQVAVLNHASIGGFVTHCGWNSVLEAVCAGVPMVAWPLYAEQRFNRVVLVEEMKLAFPMEESEEGFVTATEVEKRVRELMEWEEGNTLRLRIMAMKEAAETAMSDGGSSGTALTKLVKSWRPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E2E3</accession>
    <name>A0AA39E2E3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring)</fullName>
            <ecNumber evidence="5">1.2.4.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_005412</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[protein] + 3-methyl-2-oxobutanoate + H(+) = N(6)-[(R)-S(8)-2-methylpropanoyldihydrolipoyl]-L-lysyl-[protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:13457"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10497"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83142"/>
            <dbReference type="EC" id="1.2.4.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Heterotetramer of alpha and beta chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the BCKDHA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.4.4" evidence="5"/>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9704110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E2E3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003863">
        <property type="term" value="F:branched-chain 2-oxo acid dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009083">
        <property type="term" value="P:branched-chain amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02000">
        <property type="entry name" value="TPP_E1_PDC_ADC_BCADC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000015">
        <property type="entry name" value="2-oxoisovalerate dehydrogenase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050771">
        <property type="entry name" value="Alpha-ketoacid_DH_E1_comp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43380:SF11">
        <property type="entry name" value="2-OXOISOVALERATE DEHYDROGENASE SUBUNIT ALPHA 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43380">
        <property type="entry name" value="2-OXOISOVALERATE DEHYDROGENASE SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-0630">Potassium</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Dehydrogenase E1 component" evidence="13">
        <location>
            <begin position="144"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00052792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9704110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="481" mass="53657" checksum="03C2197E31A8097C" modified="2024-01-24" version="1">MALSLGKSKRILQCVKSKICVVGLGKNSFWSGWFRHGCPSSMAPLAPTCRKPDAVLIDAATQFATRRFESTKTEKHVDSLCDAEDNQLLDFPGGKVAFTSEMRFIPESPMERAHCYRVLDDNGQPNMSNFAQVSKEVAVKMYANMLTLQVMDTIFYEAQRQGRISFYVTSIGEEAINVASAAALSIDDVIFPQYREPGVLLWRGFTLQEFANQCFGNKADYGKGRQMPIHYGSNKHNYVTVSSTVATQIPQAVGAAYSLKMDGKDACTVTYFGDGGSSTGDFHAALNFAAVMEAPVIFICRNNGWAISTPVSDQFRSDGVVVRGRGYGVRSIRVDGNDALAMYTAVHAARKMAITEHRPILIEALTYRAGHHSTSDDSSKYRPVSEIELWRMARDPVSRLRRWIESNDWWSGEAESELRSNVRKEVLDAIQVAERVEKPPIAEIFTDVYDAPPSNLCEQEKLLRETIRRFPQDYPPDVPAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E2P3</accession>
    <name>A0AA39E2P3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cytochrome b5 heme-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_002364</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the cytochrome b5 family. MAPR subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E2P3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005496">
        <property type="term" value="F:steroid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050577">
        <property type="entry name" value="MAPR/NEUFC/NENF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10281">
        <property type="entry name" value="MEMBRANE-ASSOCIATED PROGESTERONE RECEPTOR COMPONENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10281:SF4">
        <property type="entry name" value="NEUFERRICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0754">Steroid-binding</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="5">
        <location>
            <begin position="52"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9707336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="255" mass="28058" checksum="163929BD8E027FAF" modified="2024-01-24" version="1">MVETERERERERGKVRSQTNFSGIAVPISLLVLLLGLSFSSKPSPSSPSRLFTAQELALHNGTDDSLPILLAILGSVFDVTKGKSHYGLGGGYNHFSGRDASRAFVSGNFSGDGLTDSLRGLSSTEVKSVVEWRDFYFRSYTFVGKLVGLYYDSHGNATKQLKGVEAKAARGAQLLEKQKSEEAKLPSCNSRWSQDEGGKVWCDNGYPRLIQRPLEIALTGKMSKRCACFKEDQLGQQGLEVYQGCDYHAKVCQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E3B4</accession>
    <name>A0AA39E3B4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Diacylglycerol O-acyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_003238</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a long chain fatty alcohol + a fatty acyl-CoA = a long-chain alcohol wax ester + CoA</text>
            <dbReference type="Rhea" id="RHEA:38443"/>
            <dbReference type="ChEBI" id="CHEBI:17135"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:77636"/>
            <dbReference type="ChEBI" id="CHEBI:235323"/>
            <dbReference type="EC" id="2.3.1.75"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>an acyl-CoA + a 1,2-diacyl-sn-glycerol = a triacyl-sn-glycerol + CoA</text>
            <dbReference type="Rhea" id="RHEA:10868"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:64615"/>
            <dbReference type="EC" id="2.3.1.20"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Glycerolipid metabolism; triacylglycerol biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">In the N-terminal section; belongs to the long-chain O-acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E3B4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004144">
        <property type="term" value="F:diacylglycerol O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047196">
        <property type="term" value="F:long-chain-alcohol O-fatty-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045034">
        <property type="entry name" value="O-acyltransferase_WSD1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009721">
        <property type="entry name" value="O-acyltransferase_WSD1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004255">
        <property type="entry name" value="O-acyltransferase_WSD1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31650">
        <property type="entry name" value="O-ACYLTRANSFERASE (WSD1-LIKE) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31650:SF41">
        <property type="entry name" value="O-ACYLTRANSFERASE WSD1-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06974">
        <property type="entry name" value="WS_DGAT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03007">
        <property type="entry name" value="WS_DGAT_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="181"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="O-acyltransferase WSD1-like N-terminal" evidence="12">
        <location>
            <begin position="108"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="O-acyltransferase WSD1 C-terminal" evidence="13">
        <location>
            <begin position="306"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF03007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF06974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9705085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="461" mass="51992" checksum="456240774913E01D" modified="2024-01-24" version="1">MGSPEIERDEPVTPAGRLFLRPEMDQIINCVIGAKNPFEVDAVKKEMANSMMLKHPRFCSLMVRDRHGREYWRKTEIEIDRHFIILNDSLDGDDDEDAVNGYLADLAVSSPLSTDKPLWEIHFLTAHKCAVVRIHHALGDGISLMSLLLACFRRSDCPDQLPTMGSSSQPKPRNSRRRNRLWELLNIAWFTLVFVVEFILRSLWVKDRKTTVSGGAGVELWPRKLATAKFLLDDMKTVKNAIPNGTINDVLFGVISAGLSRYLDHRSPNKLQEGIQITGVAMVNLRKQPGLQEMAELMQSKSGSRWGNKFGMLLLPVYYHKGLVDPLDYIKKAKTMIDRKKQSLEAYFSYKIGYFVMNFLGSKIACVLNYRIVCNTTFTISNVLGPREEITIVGNPVTYIRANSTGLPHAITMHMVSYAGRADLQILVAKDIIPDPKFLAKCFEEALLEMKEAAAEATSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E3K3</accession>
    <name>A0AA39E3K3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Zinc metallopeptidase EGY3, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_003399</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M50B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E3K3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031969">
        <property type="term" value="C:chloroplast membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044838">
        <property type="entry name" value="EGY1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31412:SF2">
        <property type="entry name" value="ZINC METALLOPEPTIDASE EGY3, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31412">
        <property type="entry name" value="ZINC METALLOPROTEASE EGY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0934">Plastid</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="278"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="311"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="383"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="493"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="543"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="28"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="130"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="44"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9705340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="579" mass="63342" checksum="C50D33B12A2E4528" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E496</accession>
    <name>A0AA39E496_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dirigent protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_001578</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Secreted</location>
            <location evidence="4">Extracellular space</location>
            <location evidence="4">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the plant dirigent protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E496"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009699">
        <property type="term" value="P:phenylpropanoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.480.10">
        <property type="entry name" value="Allene oxide cyclase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044859">
        <property type="entry name" value="Allene_oxi_cyc_Dirigent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004265">
        <property type="entry name" value="Dirigent"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46442">
        <property type="entry name" value="DIRIGENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46442:SF9">
        <property type="entry name" value="DIRIGENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03018">
        <property type="entry name" value="Dirigent"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0052">Apoplast</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041480152" description="Dirigent protein" evidence="4">
        <location>
            <begin position="22"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9706075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="185" mass="20935" checksum="2DB9A73B8ADD5367" modified="2024-01-24" version="1">MGARSLVVLLFLMFILSFSSAYQGKKKQYKPCKQMVLYFHDVIYNGQNAANATSAIVGAPQWGNLTILADKFHFGNVVVFDDPITLDNNFHSKPVGRAQGMYIYDTKNTFTAWLGFTFVLKSTDFQGTLNFMGADPTLVKTRDITVVSGTGDFFMHRGIATIMTDAFEDDVYFRLRVDIKLYECW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E5Z7</accession>
    <name>A0AA39E5Z7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transcription factor CBF/NF-Y/archaeal histone domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_001210</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NFYB/HAP3 subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E5Z7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016602">
        <property type="term" value="C:CCAAT-binding factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22907">
        <property type="entry name" value="HFD_NFYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027113">
        <property type="entry name" value="Transc_fact_NFYB/HAP3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11064">
        <property type="entry name" value="CCAAT-BINDING TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11064:SF83">
        <property type="entry name" value="NUCLEAR TRANSCRIPTION FACTOR Y SUBUNIT B-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00615">
        <property type="entry name" value="CCAATSUBUNTA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription factor CBF/NF-Y/archaeal histone" evidence="4">
        <location>
            <begin position="9"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9709613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="152" mass="17027" checksum="ED2F000EA0866FDC" modified="2024-01-24" version="1">MADKQDLLLPIANVGRIMKQILPPRAKVSKEAKETVQECVSEFVMFVTGEASAKCRKEDRQTVTVDDICWALSSLGLDDYAGATVRYLHKYREFERERVNQKVQNEVHSTRAKSGASNYKCIQSGKQTETPTPLLLFKVTDQNGSSSLTKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E6J5</accession>
    <name>A0AA39E6J5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transferase, transferring glycosyl groups</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_001487</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9710033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E6J5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038941">
        <property type="entry name" value="At4g14100-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33880">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33880:SF19">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041225242" description="Transferase, transferring glycosyl groups" evidence="1">
        <location>
            <begin position="27"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9710033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="218" mass="25120" checksum="C551C8A931AF168B" modified="2024-01-24" version="1">MASATKHPTILIVFLIHCLCLSRSSSKSTDPTPTPAPWPPQFNSLLYMNVNGSNQIVDLWYDWPKGRNLNIIQNQLGKRLYDLEWDNGTSFFYTLDSTQECRTKHFEVGILRPDWLDGAHYLGQREVDGFLCNVWEKVDFIWYYEDVVTKRPVHWRFFNGMSAHVMTFKVGAVREDEKWQAPVYCFGTTDPKAETTSAGNPRERLVDGVLRGSMSLSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E6W3</accession>
    <name>A0AA39E6W3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein uL15/eL18 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_000696</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E6W3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.100.10.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L27a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.100.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01341">
        <property type="entry name" value="Ribosomal_uL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030878">
        <property type="entry name" value="Ribosomal_uL15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021131">
        <property type="entry name" value="Ribosomal_uL15/eL18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036227">
        <property type="entry name" value="Ribosomal_uL15/eL18_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001196">
        <property type="entry name" value="Ribosomal_uL15_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11721">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11721:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00828">
        <property type="entry name" value="Ribosomal_L27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52080">
        <property type="entry name" value="Ribosomal proteins L15p and L18e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00475">
        <property type="entry name" value="RIBOSOMAL_L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL15/eL18" evidence="6">
        <location>
            <begin position="72"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="21"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9708804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="146" mass="16359" checksum="1BF1953934CD551E" modified="2024-01-24" version="1">MTTRFKKHRKKRGHVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVGMRYFHKLRNKFFCPIVNVDKLWSLIPQEVKAKASPENAPMIDVTQFGYVKVLGKGFLPESQPIVVKAKLISKVAEKKIKEAGGAVVLTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E8D4</accession>
    <name>A0AA39E8D4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Urea-proton symporter DUR3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">High-affinity urea active transporter DUR3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_002575</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">High-affinity urea-proton symporter involved in the active transport of urea across the plasma membrane into root cells. May play an important role in urea uptake by plant cells at low external urea concentrations.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E8D4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015204">
        <property type="term" value="F:urea transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11476">
        <property type="entry name" value="SLC5sbd_DUR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1730.10:FF:000006">
        <property type="entry name" value="Urea active transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031155">
        <property type="entry name" value="DUR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00813">
        <property type="entry name" value="sss"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46154">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46154:SF4">
        <property type="entry name" value="UREA ACTIVE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="39"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="158"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="218"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="288"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="333"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="436"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="461"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="489"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="529"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="588"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="619"/>
            <end position="643"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00059650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00072203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00079542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9707602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="701" mass="75925" checksum="993EC2BF7CB29FB9" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YGG0</accession>
    <name>A0AA38YGG0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RPW8 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_026515</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9669994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YGG0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008808">
        <property type="entry name" value="Powdery_mildew-R_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766">
        <property type="entry name" value="PLANT BROAD-SPECTRUM MILDEW RESISTANCE PROTEIN RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766:SF3">
        <property type="entry name" value="RPW8 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05659">
        <property type="entry name" value="RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51153">
        <property type="entry name" value="RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="RPW8" evidence="4">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9669994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="863" mass="97303" checksum="370FDE5B1DF90AFD" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YGR9</accession>
    <name>A0AA38YGR9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_026605</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YGR9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006970">
        <property type="term" value="P:response to osmotic stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14662">
        <property type="entry name" value="STKc_SnRK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000237">
        <property type="entry name" value="Serine/threonine-protein kinase SAPK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000085">
        <property type="entry name" value="Serine/threonine-protein kinase SRK2E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343">
        <property type="entry name" value="SERINE/THREONINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343:SF476">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE SRK2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="4"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9670157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="338" mass="38210" checksum="98D061F49E7B9148" modified="2024-01-24" version="1">MERYEILKDIGSGNFGVAKLVRDKWSGELYAVKYIERGQKIDEHVQREIMNHRSLKHPNIVRFKEVLLTPTHLAIVMEYAAGGELFGRICNAGRFSEDEARYFFQQLISGVSYCHSMQICHRDLKLENTLLDGSPAPRLKICDFGYSKSSVLHSQPKSTVGTPAYIAPEVLSRKEYDGKIADVWSCGVTLYVMLVGAYPFEDPDDPRNFRKTIARILSVHYSIPDYVRVSMECKHLLSRIFVANPDKRITIPEIQNHPWFIRNLPIEFMQGGEASAQTDYVNDPAQSVDEIVKIIQEARIEGPKVGGHFFGASMDLDDLDADADIDDIETSGDFVCAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YHG4</accession>
    <name>A0AA38YHG4_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_026811</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NPH3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YHG4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18312">
        <property type="entry name" value="BTB_POZ_NPY3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043454">
        <property type="entry name" value="NPH3/RPT2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027356">
        <property type="entry name" value="NPH3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32370">
        <property type="entry name" value="OS12G0117600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03000">
        <property type="entry name" value="NPH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51649">
        <property type="entry name" value="NPH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="BTB" evidence="5">
        <location>
            <begin position="29"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="NPH3" evidence="6">
        <location>
            <begin position="211"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="489"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="547"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="593"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="558"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="598"/>
            <end position="610"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9670505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="629" mass="69482" checksum="BF0E43B94718A0D6" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YHX8</accession>
    <name>A0AA38YHX8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PI-PLC X domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_026952</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YHX8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08588">
        <property type="entry name" value="PI-PLCc_At5g67130_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051057">
        <property type="entry name" value="PI-PLC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593:SF89">
        <property type="entry name" value="PLC-LIKE PHOSPHODIESTERASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26178">
        <property type="entry name" value="PI-PLC_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041376874" description="PI-PLC X domain-containing protein" evidence="2">
        <location>
            <begin position="28"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="367"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="373"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9670731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="410" mass="44791" checksum="744BFDE0D0F68FED" modified="2024-01-24" version="1">MMKTCNLHYPILGMFIIFGLFVGFSSSLEIGETCNGDSTCDAGLTCQTCSANGNTRPRCTRIQPVNATSKVKGLAFNKYSWLTTHNSYAKTGGSTLGPANQEDSVTNQLQNGVRGLMLDMYDFNDDIWLCHSFRGTCYNFTAFQPAINVLKEIQAFLEANPSEIVTIFIEDYVTSSQGLTKVFNASGLSKYWFPLSRMPTNGEDWPTVDDMAQQNQRLVVFTSKKSKEASEGIAYEWNYVVENQYGDGGMIAGSCPNRAESSAMNTKTRSLIIQNYFPDNPNSTEACADNSAPLISMMKTCYEAAGDRWPNFIAVDFYQRSDGGGAPEAVDEANGQLTCGCASISYCKENATFGSCDVPVLSPPPPASISPSTNVPNTSNDSSTSHRDGNLLQARWMLGTMLTAILLLWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YJR4</accession>
    <name>A0AA38YJR4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Regulator of chromosome condensation (RCC1) family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_025417</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YJR4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09917">
        <property type="entry name" value="F-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051625">
        <property type="entry name" value="Signaling_Regulatory_Domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22872">
        <property type="entry name" value="BTK-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22872:SF2">
        <property type="entry name" value="INHIBITOR OF BRUTON TYROSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00415">
        <property type="entry name" value="RCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="221"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9671691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="242" mass="26877" checksum="586E90FF9ECB5BC4" modified="2024-01-24" version="1">MANRYWSVSIEELPSHLILEILSSGRLSAVDLVCLELTSRTFGASHGLYPHKFRSLVDFAAFQLCGSHPIYVALSLNAQKDLFDRCGENWKRVLRFLQSVEQSSYISETSAGNMQITTGRYHTLLIKDSSVYSCGSSLCGVLGHGPETTQCVAFSRINFPPLAHVAQVSASHNHAAFVMQSGEVFTCGDNSSFCCGHGDTVRPIFRPRLVEALKGVPCKQVAFCCYWFFLFAFLSFYLNFSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YLG7</accession>
    <name>A0AA38YLG7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WPP domain-interacting protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_025974</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YLG7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044696">
        <property type="entry name" value="WIP1/2/3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34562:SF8">
        <property type="entry name" value="WPP DOMAIN-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34562">
        <property type="entry name" value="WPP DOMAIN-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="594"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="59"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="185"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="441"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="208"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="229"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="245"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="277"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9672569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="625" mass="68203" checksum="7FAE3D6AB7F01FF0" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YLU3</accession>
    <name>A0AA38YLU3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glutathione S-transferase</fullName>
            <ecNumber evidence="3">2.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_026170</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Is involved in the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GST superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YLU3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03185">
        <property type="entry name" value="GST_C_Tau"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03058">
        <property type="entry name" value="GST_N_Tau"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000018">
        <property type="entry name" value="Glutathione S-transferase U20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000014">
        <property type="entry name" value="Tau class glutathione S-transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045074">
        <property type="entry name" value="GST_C_Tau"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045073">
        <property type="entry name" value="Omega/Tau-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11260">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE, GST, SUPERFAMILY, GST DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11260:SF764">
        <property type="entry name" value="GLUTATHIONE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13410">
        <property type="entry name" value="GST_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01152">
        <property type="entry name" value="Main.3:_Omega-_and_Tau-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="4">
        <location>
            <begin position="5"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="5">
        <location>
            <begin position="91"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU369102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9672822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="221" mass="25257" checksum="8108E5A93921AD5B" modified="2024-01-24" version="1">MANTDHIVLLDSLGSPFGMRVRLALAAKGIEYQIEEEDQTQSKSSLLLKVNPVHKKIPVLIHNGKPICESLIIVEYIDEVWKDRCPLLPADPYQRAKAKFWADFIDKMVHPPSDKVWAANGEEQEAGKKEFIDGIKLLEGELKSNPYFGGETLGFLDIAFLPLYYRFHTFETFGKFSIEAECPKLVAWGKRCMEEEFVYASLPHPHKIYDLVVQYKKKLGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YMD6</accession>
    <name>A0AA38YMD6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_026430</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. ABCD family. Peroxisomal fatty acyl CoA transporter (TC 3.A.1.203) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9673144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YMD6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03223">
        <property type="entry name" value="ABCD_peroxisomal_ALDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050835">
        <property type="entry name" value="ABC_transporter_sub-D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11384">
        <property type="entry name" value="ATP-BINDING CASSETTE, SUB-FAMILY D MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11384:SF59">
        <property type="entry name" value="LYSOSOMAL COBALAMIN TRANSPORTER ABCD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="61"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9673144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="348" mass="38854" checksum="B30E6C49FABF8408" modified="2024-01-24" version="1">MITVLIPPMYAGEFDDILDNSSSKDLSDPSEEINLVYYDVKSLTLLESNGSMAMDQCQKLLDIENLTLLTPKSKTALIWDLSLEIYEKDHLLVTGPSGSGKTSLLRALAGLWSTGRGKITFYVKGAEDHLLGAAPLEATPSQDTYPGVEQPKSKDSRGIFFLPQRPYMVLGTLRQQLLYPTWAEDVIPMTDGDKPDGTLPFLMGVSTSENEGENPRKPTTDDIIQVLEDVRLGYILSRFNSLDSTYEWSSVLSLGEQQRLAFARLLLSKPNLVLLDESTSALDEANEAHLYQQIEAAGITYVSIGHRRTLYGYHNRLLHISAADPNSTQRNWYIEPINQDTMYNLSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YN68</accession>
    <name>A0AA38YN68_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA-(apurinic or apyrimidinic site) lyase</fullName>
            <ecNumber evidence="3">4.2.99.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_023247</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-phospho-2'-deoxyribonucleoside-DNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66592"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:16897"/>
            <dbReference type="Rhea" id="RHEA-COMP:17067"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:157695"/>
            <dbReference type="ChEBI" id="CHEBI:167181"/>
            <dbReference type="EC" id="4.2.99.18"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the type-1 OGG1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.99.18" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YN68"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034039">
        <property type="term" value="F:8-oxo-7,8-dihydroguanine DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140078">
        <property type="term" value="F:class I DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006285">
        <property type="term" value="P:base-excision repair, AP site formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.30:FF:000012">
        <property type="entry name" value="N-glycosylase/DNA lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1670.10:FF:000005">
        <property type="entry name" value="N-glycosylase/DNA lyase OGG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="entry name" value="Helix-hairpin-Helix base-excision DNA repair enzymes (C-terminal)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012904">
        <property type="entry name" value="OGG_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052054">
        <property type="entry name" value="Oxidative_DNA_repair_enzyme"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10242">
        <property type="entry name" value="8-OXOGUANINE DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10242:SF2">
        <property type="entry name" value="N-GLYCOSYLASE_DNA LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07934">
        <property type="entry name" value="OGG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55945">
        <property type="entry name" value="TATA-box binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HhH-GPD" evidence="13">
        <location>
            <begin position="184"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="374"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="16"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="34"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="379"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9673563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="399" mass="43846" checksum="E06992F33AE528C2" modified="2024-01-24" version="1">MHSLKLPRTMKRRRPIPQPPSTPPTPTLLKTLASNSKPTSNSSKKPRILLKTPQTPPPTTPISPKWVPLNIAKSELSLALTFPTGQTFRWKQTTPLQYTGVIGSHLISLKHLQNGNVAYLIHQSPSEENARSALLDFLNVGISLSEMWEVFKASDSRFAELAQYLGGARVLRQDPLECLIQFLCSSNNNIGRITKMVDFVSSLGNYLGSIGGFDFYEFPSLDRLSMVSEEDFREAGFGYRAKYIIGTVKALQSKSGGGIEWLASLREMDLQEVVDALSTLPGVGPKVAACIALFSLDQHHAIPVDTHVWQIATRYLIPELAGARLTPKLCSRVVDAFVSKYGKYAGWAQTLLFIAELPSQKTILPPKFRATEENKDTNIKASTTGNGPCGSSLSISRSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YNS0</accession>
    <name>A0AA38YNS0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CRAL-TRIO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_023377</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YNS0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008526">
        <property type="term" value="F:phosphatidylinositol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.525.10:FF:000008">
        <property type="entry name" value="Phosphatidylinositol transfer protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052578">
        <property type="entry name" value="PI_Transfer_CRAL-TRIO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45824:SF6">
        <property type="entry name" value="F16L1.9 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45824">
        <property type="entry name" value="GH16843P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03765">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01100">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="2">
        <location>
            <begin position="84"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="263"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="307"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9673787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="361" mass="41066" checksum="C980B7A831FCAB93" modified="2024-01-24" version="1">MSTGLKKSPSNGYEKPSTSKEQQEKINEVRRLIGPLPDRLSIYCSDASISRYLTARNWNVKKATKMLKETLKWRSEYKPEEIRWEDIAHEAETGKIYRANYIDKHGRTVLVMKPSCQNSKSTKGQIRYLVYCMENAILNLPPNQEQMVWMIDFQGFNLSNISVKLTRETAHVLQDHYPERLGLAILYNPPKFFEPFWTVVKPFLEPKTCNKVKFVYSDDLNAKKIMEDLFDMDKLESAFGGNDTMGFNINKYSERMREDDKKVPSFWTRDSHPSGAALQPALANDPTLTPINLQSDSDASDKESAENPPSLTHINLQSDSDASDKERAENSPSHGVNSGEGPLDNNVLVSDESRNRAVGIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YPC4</accession>
    <name>A0AA38YPC4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_023630</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YPC4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF78">
        <property type="entry name" value="OS12G0587133 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9674190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="570" mass="65729" checksum="2BA4CC3FE68F2631" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YRR6</accession>
    <name>A0AA38YRR6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Gfo/Idh/MocA-like oxidoreductase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_024400</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Gfo/Idh/MocA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YRR6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055170">
        <property type="entry name" value="GFO_IDH_MocA-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46368">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46368:SF19">
        <property type="entry name" value="GFO_IDH_MOCA-LIKE OXIDOREDUCTASE N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22725">
        <property type="entry name" value="GFO_IDH_MocA_C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase N-terminal" evidence="2">
        <location>
            <begin position="6"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="GFO/IDH/MocA-like oxidoreductase" evidence="3">
        <location>
            <begin position="148"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9675456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="363" mass="39572" checksum="9F26DDCB0868FFBB" modified="2024-01-24" version="1">MAETPIRLGILGYGEISRRLSKLSKAIALVSNATLVAIGSRRIHKATGITSADGFPSWVKMYESYEAVLDDPDVDAVYVPLPAKLQPKWAVAAAEKKKHVLLEKPVAPDVVELDRVLAACEASGVQLMDGTLWMHHPRTPEMKEFISDTQRFGQLKSIHCCFTYYANPEFLENDFRLKPDPDGLGALGDCGWYCIRAILWAADHQLPNSVTALPGSVFNNAGVILSCGASLDWADGKVATFHCSVLSNLTMEITATGTKGTLQLHDFVIPFKHDTASYSTASESSLEELSTGWKARPSEHSVANDLPQEALMMREFVGLVAGIKRDGKEPDNKWPAMTRKTQLVLDAVKASIEKGCEAVEVGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YSW4</accession>
    <name>A0AA38YSW4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase</fullName>
            <ecNumber evidence="3">3.2.2.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_024859</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>NAD(+) + H2O = ADP-D-ribose + nicotinamide + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16301"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57967"/>
            <dbReference type="EC" id="3.2.2.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:16302"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the disease resistance TIR-NB-LRR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.6" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9676065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YSW4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061809">
        <property type="term" value="F:NAD+ nucleosidase activity, cyclic ADP-ribose generating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050832">
        <property type="term" value="P:defense response to fungus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043068">
        <property type="term" value="P:positive regulation of programmed cell death"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10140:FF:000007">
        <property type="entry name" value="Disease resistance protein (TIR-NBS-LRR class)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32009:SF39">
        <property type="entry name" value="TIR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32009">
        <property type="entry name" value="TMV RESISTANCE PROTEIN N-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TIR" evidence="11">
        <location>
            <begin position="26"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9676065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="176" mass="20182" checksum="082E135424DB02D1" modified="2024-01-24" version="1">MASSSNSKRPFSSSSSSSSSSSNSKWRYDVFLSFRGEDTRKNFTSHLYKALDHANIETFKDDKELRRGEEIAPELLKAIEGSRIALIVFSKTYAHSKWCLDELAKIMECQEEKGQKVFPIFYDVEPSEVRNQTGIYGEAFNNHESNADEEKKKKIEKWRIALRKAGNLSGFPLQDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YUW8</accession>
    <name>A0AA38YUW8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">H/ACA ribonucleoprotein complex subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Nucleolar protein family A member 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_022248</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Common component of the spliceosome and rRNA processing machinery.</text>
    </comment>
    <comment type="function">
        <text evidence="8">Required for ribosome biogenesis. Part of a complex which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Pseudouridine ('psi') residues may serve to stabilize the conformation of rRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
            <location evidence="1 8">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the eukaryotic ribosomal protein eL8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YUW8"/>
    <dbReference type="SMR" id="A0AA38YUW8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031429">
        <property type="term" value="C:box H/ACA snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031120">
        <property type="term" value="P:snRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000015">
        <property type="entry name" value="H/ACA ribonucleoprotein complex subunit NHP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050257">
        <property type="entry name" value="eL8/uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002415">
        <property type="entry name" value="H/ACA_rnp_Nhp2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018492">
        <property type="entry name" value="Ribosomal_eL8/Nhp2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23105">
        <property type="entry name" value="RIBOSOMAL PROTEIN L7AE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00881">
        <property type="entry name" value="L7ARS6FAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00883">
        <property type="entry name" value="NUCLEARHMG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="9">
        <location>
            <begin position="40"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9677151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="157" mass="17143" checksum="A005D1D6C961FFE6" modified="2024-01-24" version="1">MGSDSEAERSAHKEKEKKKLSALAPIAKPLAGKKLCKRTLKLIRRAAEHKCLKRGVKEVVKGIRRGDKGFCVIAGNITPIDVITHVPILCEEADIPYVYVPSKEDLANAGSTKRPTCCVLVLTKPTKGELGQEEQEKLKAEYTQVVSDVSGLTSTLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YV20</accession>
    <name>A0AA38YV20_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">UDENN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_022180</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YV20"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032483">
        <property type="term" value="P:regulation of Rab protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000360">
        <property type="entry name" value="DENN domain and WD repeat-containing protein SCD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000624">
        <property type="entry name" value="DENN domain and WD repeat-containing protein SCD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.200:FF:000006">
        <property type="entry name" value="DENN domain and WD repeat-containing protein SCD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11500:FF:000007">
        <property type="entry name" value="DENN domain and WD repeat-containing protein SCD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001194">
        <property type="entry name" value="cDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005112">
        <property type="entry name" value="dDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043153">
        <property type="entry name" value="DENN_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051696">
        <property type="entry name" value="DENN_Domain_GEFs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037516">
        <property type="entry name" value="Tripartite_DENN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005113">
        <property type="entry name" value="uDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12296:SF21">
        <property type="entry name" value="DENN DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12296">
        <property type="entry name" value="DENN DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02141">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03456">
        <property type="entry name" value="uDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00801">
        <property type="entry name" value="dDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00799">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00800">
        <property type="entry name" value="uDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50211">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="UDENN" evidence="5">
        <location>
            <begin position="19"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="950"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="990"/>
            <end position="1030"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1035"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1073"/>
            <end position="1112"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1113"/>
            <end position="1145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="517"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="777"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="520"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="535"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9677057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1249" mass="138020" checksum="C62AEFCB4214575A" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YV97</accession>
    <name>A0AA38YV97_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_022265</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YV97"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9677177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="119" mass="12521" checksum="374C2EEABCFE33D5" modified="2024-01-24" version="1">MAKSLSSWRRVGNLELIQLSTMVHRRESENAVQDKTIVGDEGYRVWKAITKADAMQLVTIIFGQFSFLSTSVGITGDILILISSPSSAISDSRVAGINGLSGGDGGGATSCDVRSGVTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YVS4</accession>
    <name>A0AA38YVS4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">malate dehydrogenase</fullName>
            <ecNumber evidence="3">1.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_022422</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(S)-malate + NAD(+) = oxaloacetate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21432"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.37"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the LDH/MDH superfamily. MDH type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.37" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YVS4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030060">
        <property type="term" value="F:L-malate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006108">
        <property type="term" value="P:malate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01337">
        <property type="entry name" value="MDH_glyoxysomal_mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000017">
        <property type="entry name" value="Malate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.110.10:FF:000001">
        <property type="entry name" value="Malate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.110.10">
        <property type="entry name" value="Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022383">
        <property type="entry name" value="Lactate/malate_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001236">
        <property type="entry name" value="Lactate/malate_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015955">
        <property type="entry name" value="Lactate_DH/Glyco_Ohase_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001252">
        <property type="entry name" value="Malate_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010097">
        <property type="entry name" value="Malate_DH_type1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01772">
        <property type="entry name" value="MDH_euk_gproteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11540">
        <property type="entry name" value="MALATE AND LACTATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11540:SF16">
        <property type="entry name" value="MALATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02866">
        <property type="entry name" value="Ldh_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00056">
        <property type="entry name" value="Ldh_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56327">
        <property type="entry name" value="LDH C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00068">
        <property type="entry name" value="MDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Lactate/malate dehydrogenase N-terminal" evidence="8">
        <location>
            <begin position="96"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="Lactate/malate dehydrogenase C-terminal" evidence="9">
        <location>
            <begin position="240"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9677427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="413" mass="43437" checksum="7BAB586574E32CC7" modified="2024-01-24" version="1">MAATSSTAITIGSSASIGSKAIPLSRSKRFGVSVNSQNCLKSFSGLKAASSISCESEASFLGKESSAALRNSFAPKAGKQNLRHQYYLQPQASSSKVAILGAAGGIGQPLALLIKMSPLVSALHLYDIANVKGVAADLSHCNTPAQVLDFTGTSELANSLKGVDVVVIPAGVPRKPGMTRDDLFNINANIVKTLVEAVADNCPDAFIHIISNPVNSTVPIAVEVLKRKGVYDPKKVFGVTTLDVVRANTFVAQKKNLRLIDVDVPVVGGHAGITILPLLSKTKPSVSFTDEEVEELTVRIQNAGTEVVEAKAGAGSATLSMAYAAARFVESSLRALDGDTDVYECAYVQSELTELPFFASRVKIGKKGIEAVISSDLQGLTEYEEKALEALKPELKASIEKGVAFAQKQTVTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YVT3</accession>
    <name>A0AA38YVT3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_022446</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YVT3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000558">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At5g39710"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050872">
        <property type="entry name" value="PPR_P_subfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46128">
        <property type="entry name" value="MITOCHONDRIAL GROUP I INTRON SPLICING FACTOR CCM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46128:SF82">
        <property type="entry name" value="PENTACOTRIPEPTIDE-REPEAT REGION OF PRORP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="11"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="157"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="192"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="227"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="262"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="297"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="332"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="367"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="402"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="437"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="472"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="555"/>
            <end position="589"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9677462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="665" mass="73773" checksum="3BE500B30F87E732" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YWG0</accession>
    <name>A0AA38YWG0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">peptide-methionine (S)-S-oxide reductase</fullName>
            <ecNumber evidence="2">1.8.4.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Peptide-methionine (S)-S-oxide reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Protein-methionine-S-oxide reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_022752</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MsrA Met sulfoxide reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.8.4.11" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YWG0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008113">
        <property type="term" value="F:peptide-methionine (S)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1060.10:FF:000002">
        <property type="entry name" value="Peptide methionine sulfoxide reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1060.10">
        <property type="entry name" value="Peptide methionine sulphoxide reductase MsrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01401">
        <property type="entry name" value="MsrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002569">
        <property type="entry name" value="Met_Sox_Rdtase_MsrA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036509">
        <property type="entry name" value="Met_Sox_Rdtase_MsrA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050162">
        <property type="entry name" value="MsrA_MetSO_reductase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00401">
        <property type="entry name" value="msrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42799">
        <property type="entry name" value="MITOCHONDRIAL PEPTIDE METHIONINE SULFOXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42799:SF18">
        <property type="entry name" value="PEPTIDE-METHIONINE (S)-S-OXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01625">
        <property type="entry name" value="PMSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55068">
        <property type="entry name" value="Peptide methionine sulfoxide reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptide methionine sulphoxide reductase MsrA" evidence="6">
        <location>
            <begin position="25"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00030273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00030643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9677961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="190" mass="21214" checksum="8A864C06F9D67F39" modified="2024-01-24" version="1">MAANSSNPALDLDPDRPDHPGLELAQFGAGCFWGVELAFQRLVGVVKTEVGFSQGHVPDPTYKLVCTGTTNHVEVIRVQFDPDVCPYSNLLSLFWSRHDPTSLNRQGADVGTQYRSGIYYYNEAQARLAHESLEAKQKELNDKKIVTEILPAKRFYRAEEYHQQYLEKGGGKGLCQSAEKGCTDPIRCYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YWV8</accession>
    <name>A0AA38YWV8_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_022730</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YWV8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9677924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="105" mass="12056" checksum="7E204FF08CBA3646" modified="2024-01-24" version="1">MDHFFTPNAEMVVQNQRSGKMNQTTINNAYKKEAKERACMLITRWMYEADIPFNVVTYPSFQPMIEAIGQYGVGVKGLTLHEVSVTNLKKELALTKDLMKDHMVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YWZ5</accession>
    <name>A0AA38YWZ5_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_022912</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9678169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YWZ5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032567">
        <property type="entry name" value="RTL1-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15503">
        <property type="entry name" value="LDOC1 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15503:SF45">
        <property type="entry name" value="RNA-DIRECTED DNA POLYMERASE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08284">
        <property type="entry name" value="RVP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9678169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="142" mass="15211" checksum="3AC21C7852F7EF0B" modified="2024-01-24" version="1">MKIKGTIGLKEAIILVDSGATHNFLSLDLVQQLALPLTTTTSYGVMMGTGISVKGKGICRGVCISMQGLTVVEDFLPLELGNTDVILGMPWLGTLGDVKVNWKMITMKIKMGKVVIVLKGDPSLSGTEVFLKAMVRALQHHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YZ56</accession>
    <name>A0AA38YZ56_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_021353</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YZ56"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000649">
        <property type="entry name" value="Leucine Rich Repeat family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001347">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046956">
        <property type="entry name" value="RLP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063">
        <property type="entry name" value="LRR RECEPTOR-LIKE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063:SF98">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE FLS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041369237" description="Leucine-rich repeat-containing N-terminal plant-type domain-containing protein" evidence="13">
        <location>
            <begin position="27"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="14">
        <location>
            <begin position="33"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="15">
        <location>
            <begin position="236"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="795"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="803"/>
            <end position="813"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9679396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="852" mass="94145" checksum="55693303C187AB43" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YZX9</accession>
    <name>A0AA38YZX9_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_021535</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YZX9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046956">
        <property type="entry name" value="RLP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063:SF103">
        <property type="entry name" value="LEUCINE-RICH RECEPTOR-LIKE KINASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063">
        <property type="entry name" value="LRR RECEPTOR-LIKE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="646"/>
            <end position="669"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9679637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="678" mass="75367" checksum="3FCD2FE02024E442" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2025-10-08" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z4F5</accession>
    <name>A0AA38Z4F5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_018197</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057135">
        <property type="entry name" value="At4g27190-like_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050905">
        <property type="entry name" value="Plant_NBS-LRR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463:SF198">
        <property type="entry name" value="RPP4C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23247">
        <property type="entry name" value="LRR_RPS2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="167"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="1069"/>
            <end position="1207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9682203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1981" mass="226111" checksum="BF10E4182CF1C803" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z4T5</accession>
    <name>A0AA38Z4T5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cysteine synthase</fullName>
            <ecNumber evidence="8">2.5.1.47</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_018252</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate</text>
            <dbReference type="Rhea" id="RHEA:14829"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:58340"/>
            <dbReference type="EC" id="2.5.1.47"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7 8">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the cysteine synthase/cystathionine beta-synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.47" evidence="8"/>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z4T5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004124">
        <property type="term" value="F:cysteine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01561">
        <property type="entry name" value="CBS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000006">
        <property type="entry name" value="Cysteine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000130">
        <property type="entry name" value="Cysteine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005856">
        <property type="entry name" value="Cys_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050214">
        <property type="entry name" value="Cys_Synth/Cystath_Beta-Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005859">
        <property type="entry name" value="CysK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001216">
        <property type="entry name" value="P-phosphate_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01139">
        <property type="entry name" value="cysK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01136">
        <property type="entry name" value="cysKM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10314">
        <property type="entry name" value="CYSTATHIONINE BETA-SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00901">
        <property type="entry name" value="CYS_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 8" id="KW-0198">Cysteine biosynthesis</keyword>
    <keyword evidence="5 7 8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="9">
        <location>
            <begin position="8"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="7">
        <location>
            <position position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR605856-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9682280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="313" mass="33606" checksum="E2593E46E4638D42" modified="2024-01-24" version="1">MEDIGKDVTELIGNTPLVYLNKVVDGCLARIAVKLEAMEPCSSVKDRIALSMIKGAEDKGLIQPGKTVLIEPTSGNSGIGLAYIAAVKGYKLILAMPASMSIERRIVLRAFGDELHLTDPVKGIKGMHEKAELLEKTPNGYMLKQFANPAKPKIHYETRPEIWRGCGGEVDVFVSGVGPGEELGRFLKEKNPDIKVYGVELAESAVLNGGEPALHKIQAIDPGFITPILDVSILDEVISSEEAIETAKLLALKEGLLVGISSGAAAAAAIKIAKRPENAGKLIVVVFPSFGERCLSTVLFDSIRQEAENMTFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z6K7</accession>
    <name>A0AA38Z6K7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_019096</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z6K7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05574">
        <property type="entry name" value="STKc_phototropin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000020">
        <property type="entry name" value="serine/threonine-protein kinase D6PK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000028">
        <property type="entry name" value="serine/threonine-protein kinase D6PK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000032">
        <property type="entry name" value="Serine/threonine-protein kinase D6PK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45637">
        <property type="entry name" value="FLIPPASE KINASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="286"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="24"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="238"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="24"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="238"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9683370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="678" mass="74704" checksum="068B3367373D61A1" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z7V3</accession>
    <name>A0AA38Z7V3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DOG1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_016379</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9683852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z7V3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051886">
        <property type="entry name" value="Seed_Dev/Stress_Resp_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025422">
        <property type="entry name" value="TGA_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46354">
        <property type="entry name" value="DOG1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46354:SF10">
        <property type="entry name" value="TRANSCRIPTION FACTOR TGA5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14144">
        <property type="entry name" value="DOG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51806">
        <property type="entry name" value="DOG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DOG1" evidence="1">
        <location>
            <begin position="10"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9683852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="240" mass="27958" checksum="2A8E560078966706" modified="2024-01-24" version="1">MPPANNNPNPEPFEIFFRGWLVRHEEVRQLLQQAAERDCDETREDEEARMQELIGRVVAHYAEYYKAKQRVVREDVMTLLEPPWLTPFERSLLWIAGFMPGFAFRLVTNCVKDLTGEQTRMMEQLRTETAAEEMDLTADLLKVQRSPTMISLVEMATRGREWADGERDAVKEQIEMVKLAMEMLVECADCLRYKTALKIMEILNPSQNVKFLLAITGLQLRVRNWGLQREAESRNDNSPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z836</accession>
    <name>A0AA38Z836_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF674 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_016570</name>
        <name evidence="2" type="ORF">PVL29_016577</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z836"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007750">
        <property type="entry name" value="DUF674"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33103">
        <property type="entry name" value="OS01G0153900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33103:SF19">
        <property type="entry name" value="OS09G0544700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05056">
        <property type="entry name" value="DUF674"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9684142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9684153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="174" mass="19638" checksum="5636E89BDACB15D8" modified="2024-01-24" version="1">MATKAAPKMSLKLLINKKDNKVVFAETEKDFIDFLFNLLTLPIGTIVSFLPKENCLRNLHESINKLEEAYLLPHQSMDSPLKLNTPIDAILPFFDLPLSWSDRHVKDDLVTYMVANDLSVTPMSMTSTMALFKKYNISEVGVLEEKVVAIGFEEALLLLHCALHSKEALTKVFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z8U4</accession>
    <name>A0AA38Z8U4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF674 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_016573</name>
        <name evidence="2" type="ORF">PVL29_016580</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z8U4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007750">
        <property type="entry name" value="DUF674"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33103">
        <property type="entry name" value="OS01G0153900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33103:SF19">
        <property type="entry name" value="OS09G0544700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05056">
        <property type="entry name" value="DUF674"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9684147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9684157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="314" mass="34998" checksum="7476149D8C0F5102" modified="2024-01-24" version="1">MASNPSTPKMSLKLLINKKDDKVVFAEAEKDFVDFLFNLLTLPIGTTTSFLSKGICLGGLHESIKKLDGAYLQPNQSADSPLKLNTLAYAILPFFNSSSSRSHGYVQGLVTYMVTDDLSVTPMSMATTMALFKKYNIEEVDVLEEKVVAIGLDEALQLLKGALQSKAALTKAFLEKEETKTNVNHRMDYQPLIIEPASHRIDHRPSTIKPPSYQIDCRPSTIKPPSHQIYRRPLTLELPSHQIDCQPSTIEPPSHQIYRQPLTLELPSHQIDCQPSTIEPPSHQIDCKPPKSATTLVTIQIKPRRSLSCGCVMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z9I7</accession>
    <name>A0AA38Z9I7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Trichome birefringence-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_017085</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PC-esterase family. TBL subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z9I7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016413">
        <property type="term" value="F:O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029962">
        <property type="entry name" value="TBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026057">
        <property type="entry name" value="TBL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025846">
        <property type="entry name" value="TBL_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285:SF155">
        <property type="entry name" value="PROTEIN TRICHOME BIREFRINGENCE-LIKE 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285">
        <property type="entry name" value="PROTEIN TRICHOME BIREFRINGENCE-LIKE 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13839">
        <property type="entry name" value="PC-Esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14416">
        <property type="entry name" value="PMR5N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041372386" description="Trichome birefringence-like N-terminal domain-containing protein" evidence="7">
        <location>
            <begin position="28"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="Trichome birefringence-like N-terminal" evidence="9">
        <location>
            <begin position="62"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="Trichome birefringence-like C-terminal" evidence="8">
        <location>
            <begin position="114"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF14416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9684921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="388" mass="45299" checksum="FF8F04BFDED1C073" modified="2024-01-24" version="1">MAKCSSICPKVLLLLLVFFTPFQLSFFHGKSSEFDLEDEAWLEEKDDEVNMVQSGHDSLGSSCEFSDGKWVYDLSYPLYDSSCPYLSTPVTCQKNGRPDSDYEKWRWKPHGCSIPRFDALHFLGRMRRKRIMLVGDSIMRNQWESLVCLVQGVIPTGRKTVTYDGPSMTFHALDFETSIEFCWAPFLVELKKGPQNKRILHLDLIEENAKYWRGVDVLVYDSAHWWTHSDKWSSWDYYMEGNTVLRSMNPMVAYQKGLTTWAKWVDLNLDPHKTRVIFRSVSPRHNRQNGWKCYNQKQPLEFFSHQLHVPEQMVVLKGVLKGMRFPVYLQDITMMSALRKDGHPSVYTRAMDQEQKQHPRDFTSDCSHWCLPGVPDAWNEMLSALLQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZA27</accession>
    <name>A0AA38ZA27_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Beta-catenin-like protein 1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_017196</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZA27"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000245">
        <property type="entry name" value="Beta-catenin-like protein 1 isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039678">
        <property type="entry name" value="CTNNBL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013180">
        <property type="entry name" value="CTNNBL1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14978:SF0">
        <property type="entry name" value="BETA-CATENIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14978">
        <property type="entry name" value="BETA-CATENIN-LIKE PROTEIN 1 NUCLEAR ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08216">
        <property type="entry name" value="CTNNBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01156">
        <property type="entry name" value="DUF1716"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Beta-catenin-like protein 1 N-terminal" evidence="6">
        <location>
            <begin position="35"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9685069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="540" mass="61645" checksum="B5F489A0F82316E7" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZAF6</accession>
    <name>A0AA38ZAF6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF4216 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_017112</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZAF6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025312">
        <property type="entry name" value="DUF4216"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48258">
        <property type="entry name" value="DUF4218 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48258:SF3">
        <property type="entry name" value="FK506-BINDING PROTEIN 4-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13952">
        <property type="entry name" value="DUF4216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4216" evidence="1">
        <location>
            <begin position="2"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9684958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="111" mass="12804" checksum="9D20184B72B03493" modified="2024-01-24" version="1">MFRILVFKCDWVDNSNGIKVDDLGFTLVDFNKTAHKSYPFILASQAKQVFYVQDQHDQRWSVVLSTPQKDFLDRERGDDLVDNFVEHQPFIGALPQVEAFNAMDDSDAICM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZC69</accession>
    <name>A0AA38ZC69_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Chromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_015160</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZC69"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045358">
        <property type="entry name" value="Ty3_capsid"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19259">
        <property type="entry name" value="Ty3_capsid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chromo" evidence="2">
        <location>
            <begin position="483"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="318"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="456"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="318"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="353"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="460"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9686135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="546" mass="62658" checksum="DE317929459C1908" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZE10</accession>
    <name>A0AA38ZE10_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_015857</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZE10"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47447">
        <property type="entry name" value="OS03G0856100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47447:SF28">
        <property type="entry name" value="PENTACOTRIPEPTIDE-REPEAT REGION OF PRORP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="181"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="216"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="251"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="286"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="321"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="356"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="426"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9687167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="482" mass="54345" checksum="0922CC60F999BBC6" modified="2024-01-24" version="1">MKMAMNKLRKTLPKPIHPPSQFTPPTASPNLIPSTPIPTLSLANTSHHQLHQFLKAHLTPSFTPQDLLIFLRNKLHYHPKFAHFDLHIFTWASTLDSFRHDHSTYEWMVKTLAITDRFDDPHSLLQGMVSNPCPCSDGIFSCPRSEPIFRFALNAFCRVGRLEDAEIAFASMRRMIDGKPSAAMYNILINGYVKCREHEKVMTVYEKMIKNRVMPDVFTFNSLISSCCRNSQFGLALEMFKEMKDKGCSPNVVSFNTLIKGSFRERKFEEGIGMAYEMLELGCEFSNVTCEILVDGLCREGRVLEACDLLIDFSGKGALPNGFDYFCLIEALCGEENVDRALELLDQLWGKGNAPSSIACTTLIEGLRRFGKTEEAFQLMEKMLREGILPDSVTCNCLLQDLCEVGRSFEANELRLLASSKGLDSDGMTFNILVNGYSREGKRKEGKRLLDKMLDLGFIPDLASYNRLMNGLSNGGSSHQHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZEB2</accession>
    <name>A0AA38ZEB2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">FAS1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_015761</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">May be a cell surface adhesion protein.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the fasciclin-like AGP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZEB2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048364">
        <property type="term" value="P:root development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048367">
        <property type="term" value="P:shoot system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.180.10:FF:000008">
        <property type="entry name" value="Fasciclin-like arabinogalactan protein 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.180.10:FF:000010">
        <property type="entry name" value="Fasciclin-like arabinogalactan protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.180.10">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036378">
        <property type="entry name" value="FAS1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000782">
        <property type="entry name" value="FAS1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033254">
        <property type="entry name" value="Plant_FLA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32382">
        <property type="entry name" value="FASCICLIN-LIKE ARABINOGALACTAN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32382:SF4">
        <property type="entry name" value="FASCICLIN-LIKE ARABINOGALACTAN PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02469">
        <property type="entry name" value="Fasciclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00554">
        <property type="entry name" value="FAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82153">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50213">
        <property type="entry name" value="FAS1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0654">Proteoglycan</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041254029" description="FAS1 domain-containing protein" evidence="13">
        <location>
            <begin position="27"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="14">
        <location>
            <begin position="26"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="14">
        <location>
            <begin position="185"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="334"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="359"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9687027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="407" mass="43670" checksum="81E9FC5D19D54011" modified="2024-01-24" version="1">MQHAPVFTSVLTLSVCLLFLSCATHAHNITHILAKHPEFSTFNHYLTITHLAGEINRRQTITVLAVSNAGMADLLSKHLSVFVIKNVLSLHVLVDYFGARKLHQITDGTASSATLFQSTGSAPGTAGFVNITDLKHGKVGFGAQDSGHLNSYFVKSVEEIPYNISVLEISQILTSAVAEAPAEAPSLNLTNMLTKQGCKAFGDLLEASGAIKTFEENVDGGLTVFCPSDDVIKGFMPKYKNLTAAGKVSLLLYHGIPIYQSMQMLKSSNGLTNTLATDGAKKYDFTVQTEGEDITLETKVVTARVTGTVLDQEPVVLYKVNKVLLPRELFKGTVEDDEAESPKASSPKSSKSKKADTADSPDADSPESDDSEDSTADQDDENGSGRFDGGRWASVLLTVFAGAFVLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZHJ0</accession>
    <name>A0AA38ZHJ0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_014417</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZHJ0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000041">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000213">
        <property type="entry name" value="Tyrosine-sulfated glycopeptide receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051502">
        <property type="entry name" value="RLP_Defense_Trigger"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48062:SF21">
        <property type="entry name" value="RECEPTOR-LIKE PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48062">
        <property type="entry name" value="RECEPTOR-LIKE PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="898"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="13">
        <location>
            <begin position="29"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9688752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="944" mass="104516" checksum="DC817BC5C80A22F3" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZJQ2</accession>
    <name>A0AA38ZJQ2_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_012716</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZJQ2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01098">
        <property type="entry name" value="PAN_AP_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000467">
        <property type="entry name" value="Liguleless narrow1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002:SF1095">
        <property type="entry name" value="G-TYPE LECTIN S-RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE RKS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002">
        <property type="entry name" value="RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD1-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08276">
        <property type="entry name" value="PAN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00473">
        <property type="entry name" value="PAN_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="206"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="8">
        <location>
            <begin position="106"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="149"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9690197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="490" mass="55046" checksum="2128435643BDE728" modified="2024-01-24" version="1">MVTEMLTTFIFNIRFWNTGDEVSMEFTLVNSSTFSSIKLGSDGLYQRYTDPCDNYGRCGPNSNCDVYTGAGFECTCLAGFEPKSQRDWSLRDGSGGCVRIRGTNTCRSGEGFIKIAGVKPPDASTARVNESLNLEGCKKECLNDCNCRAYTGANVSAGASGCLSWYGDLMDIRTLAQGGQDLFVRVDAIILAENERKKTFFHKKRMIVILAVGVVFLMIPTICSSWLIMKKRKATRLKHYSKAKEIDENGENPKLQFFDLNCNETKKSMLTWRKRFEIIIGIARGILYLHQDSRLRIIHRDLKASNILLDIDMIPKFSDFGMARLFGKNQIEGSTKRVVGTYGYMSPKYAMEGLFSIKSDVYSFGVLLLEIIIRRRNIAYYYDSPSFNLVRYVWSLWKEGKALDIVDPSLEKSNHANEVLRCVQIGLLCVQDSAIDRPTMLTIIFMLGNNSTLPPPNQPAFVMKTCHNGANSFSVVVNSVNDVTISMDAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZL01</accession>
    <name>A0AA38ZL01_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">VQ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_013274</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZL01"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008889">
        <property type="entry name" value="VQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039608">
        <property type="entry name" value="VQ_1/10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34777:SF25">
        <property type="entry name" value="VQ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34777">
        <property type="entry name" value="VQ MOTIF-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05678">
        <property type="entry name" value="VQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VQ" evidence="1">
        <location>
            <begin position="16"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9691026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="96" mass="10836" checksum="9B5F92332B6AD3AF" modified="2024-01-24" version="1">MDCKNQKPVKVVIIGTQYIETNPLSFKSVVQSLTGKDSCVAWIEESSYKSRKRRRTVDKPSGDAPAELSNCRSFNDWDGLMLELPPAEELSWVWGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZL92</accession>
    <name>A0AA38ZL92_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Homeobox-leucine zipper protein ATHB-14-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_013373</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HD-ZIP homeobox family. Class III subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZL92"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010014">
        <property type="term" value="P:meristem initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14686">
        <property type="entry name" value="bZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08875">
        <property type="entry name" value="START_ArGLABRA2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.530.20:FF:000020">
        <property type="entry name" value="homeobox-leucine zipper protein ATHB-15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000197">
        <property type="entry name" value="Homeobox-leucine zipper protein REVOLUTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044830">
        <property type="entry name" value="HD-Zip_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013978">
        <property type="entry name" value="MEKHLA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002913">
        <property type="entry name" value="START_lipid-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45950">
        <property type="entry name" value="HOMEOBOX-LEUCINE ZIPPER PROTEIN ATHB-14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45950:SF7">
        <property type="entry name" value="HOMEOBOX-LEUCINE ZIPPER PROTEIN ATHB-14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08670">
        <property type="entry name" value="MEKHLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01852">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00234">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50848">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0221">Differentiation</keyword>
    <keyword evidence="6 10 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 10 11" id="KW-0371">Homeobox</keyword>
    <keyword evidence="9 10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="14">
        <location>
            <begin position="12"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="START" evidence="15">
        <location>
            <begin position="156"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="10">
        <location>
            <begin position="14"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="131"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="86"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="131"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9691171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="867" mass="95423" checksum="185B8527D9F96CF6" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZLK0</accession>
    <name>A0AA38ZLK0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="8">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_013308</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="domain">
        <text evidence="8">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the DEAD box helicase family. DDX51/DBP6 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="8"/>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZLK0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd17956">
        <property type="entry name" value="DEADc_DDX51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001539">
        <property type="entry name" value="ATP-dependent RNA helicase DDX51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 8" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="63"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="322"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="494"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="503"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9691070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="515" mass="57480" checksum="883BC4018F381C0C" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZMJ5</accession>
    <name>A0AA38ZMJ5_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_013247</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZMJ5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36350:SF2">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36350">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9690989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="335" mass="38493" checksum="C268FDB1AEE86E44" modified="2024-01-24" version="1">MESTIGLRFSTLPNQYSICMPSGFHAARATKPHLSFSHSSLSLPQSSRNRPIEHIRGPITCMLDGSLPPTYYRVDKGRESMASASPFLHNIGSGRWTNHKGNAAQIDGHSLARVPLPGTTSIDHYIELMELAKKLKESRQYDLAVEILKLHEPKVSEDPEAAYQVQMEIMHILYLQEKFTEAMVYAEKLYDNKAIYAKRFQFELFYATKLLEIAKKLKESQHYDLAVKLLKVHDPEVENYPDASYSVKMEIMHMLFLQEKYEEAWTYCDTLSKKEVIYFPQFKSGLFYALKSGILCGLNRHEEAIKFMHKFNQNRTEEVPLATPEKDESTKNVGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZMM4</accession>
    <name>A0AA38ZMM4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">pyruvate dehydrogenase (acetyl-transferring)</fullName>
            <ecNumber evidence="4">1.2.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_013280</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[protein] + pyruvate + H(+) = N(6)-[(R)-S(8)-acetyldihydrolipoyl]-L-lysyl-[protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:19189"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10478"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83111"/>
            <dbReference type="EC" id="1.2.4.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Tetramer of 2 alpha and 2 beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.4.1" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZMM4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004739">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07036">
        <property type="entry name" value="TPP_PYR_E1-PDHc-beta_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000001">
        <property type="entry name" value="Pyruvate dehydrogenase E1 beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.920:FF:000006">
        <property type="entry name" value="Pyruvate dehydrogenase E1 component subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006667">
        <property type="entry name" value="PRK09212.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43257">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43257:SF2">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0934">Plastid</keyword>
    <keyword evidence="9" id="KW-0630">Potassium</keyword>
    <keyword evidence="12" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="15">
        <location>
            <begin position="82"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00025211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00051231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9691034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="402" mass="43729" checksum="3A0B13D11CB26D3C" modified="2024-01-24" version="1">MAAIFQGIGAAAALPSTEKFHSQSRRFVSARKGSLFVVRSDGRPSLGSSPRSRGAQHLITNAVAAKADASATSTASKPGHELLLFEALREGLEEEMDRDPRVCVMGEDVGHYGGSYKVTKGLATKYGDLRVLDTPIAENSFTGMGIGAAMTGLRPIIEGMNMGFLLLAFNQISNNCGMLHYTSGGQFKIPVVIRGPGGVGRQLGAEHSQRLESYFQSIPGIQMVACSTPYNAKGLMKAAIRSENPVILFEHVLLYNLKERIPDEEYVLSLEEAEMVRPGEHVTILTYSRMRYHVMQAAKTLVNKGYDPEVIDIRSLKPFDLYTIGNSVKKTHRVLIVEECMRTGGIGASLTAAITENFIDYLDAPIVCLSSQDVPTPYAGTLEEWTVVQPAQIVTAVEQLCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZNQ0</accession>
    <name>A0AA38ZNQ0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PVL29_011323</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZNQ0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000129">
        <property type="entry name" value="Leucine-rich repeat receptor-like kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000095">
        <property type="entry name" value="protein STRUBBELIG-RECEPTOR FAMILY 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000039">
        <property type="entry name" value="receptor-like protein kinase FERONIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024788">
        <property type="entry name" value="Malectin-like_Carb-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45631">
        <property type="entry name" value="OS07G0107800 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45631:SF206">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12819">
        <property type="entry name" value="Malectin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7 18" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041358460" description="non-specific serine/threonine protein kinase" evidence="18">
        <location>
            <begin position="23"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="513"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="549"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="607"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="635"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAJ9692194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="912" mass="101817" checksum="EF361F13753A6B67" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZQ08</accession>
    <name>A0AA38ZQ08_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_012047</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9693136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZQ08"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9693136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="114" mass="13098" checksum="5308AA5F4835F10A" modified="2024-01-24" version="1">MPRARNRLTIRPRRTTQSSPISHGNVIEGRAPIYPTVGSKCNNVSLIREFEALTPPIFRGGPNFLEAKNWMKEIKKILNIMAVPKEMRVSLASFMLRDEVDNWWDMIKTTQDAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZSP9</accession>
    <name>A0AA38ZSP9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WAT1-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_010006</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the drug/metabolite transporter (DMT) superfamily. Plant drug/metabolite exporter (P-DME) (TC 2.A.7.4) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZSP9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030184">
        <property type="entry name" value="WAT1-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31218">
        <property type="entry name" value="WAT1-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="17"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="190"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="222"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="254"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="286"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="311"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="8">
        <location>
            <begin position="17"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="8">
        <location>
            <begin position="192"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="363"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9694294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="396" mass="43561" checksum="DC75AA78CE6BA673" modified="2024-01-24" version="1">MEGKGCLGNFFQRCKPYIAMISLQFGYAGMNIITKVSLNRGMSHYVLVVYRHAFATAVIAPFALVLERKVRPKITLPIFLQLFVLGLLGPVIDQNFYYAGLKFTSPTFSCAMSNMLPAMTFVMAVLCRMEKLDMKKVRCQAKVVGTVVTVAGAMLMTLYKGPIVEMVWSHYVHPHKTNGAENSGSSDKDWLKGSILLIIATFAWASFFILQAITMRRYQAHLSLTSIVCFLGTLQSIAVTFVMEHKPSVWTIGWDMNLLAAAYAGIVSSSIAYYVQGLVMQKRGPVFVTAFSPLMMIIVAIMGSFILAEKIFLGGVIGAVLIVAGLYSVLWGKYKEYKEKEAETIPEAIKGAGEDGGMKTVMEETEEDNDIEMQKSQANKLSTPAVAVQSVEAPKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZWZ3</accession>
    <name>A0AA38ZWZ3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">SANT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_008577</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZWZ3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000374">
        <property type="entry name" value="Arginine-glutamic acid dipeptide repeat protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056067">
        <property type="entry name" value="DUF7650"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057712">
        <property type="entry name" value="DUF7952"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13859">
        <property type="entry name" value="ATROPHIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13859:SF11">
        <property type="entry name" value="GRUNGE, ISOFORM J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24662">
        <property type="entry name" value="DUF7650"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25826">
        <property type="entry name" value="DUF7952"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="DUF7952" evidence="7">
        <location>
            <begin position="187"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7650" evidence="6">
        <location>
            <begin position="355"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="648"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="686"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="755"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="648"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="706"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="755"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="773"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="825"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="843"/>
            <end position="853"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF24662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF25826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9696425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="881" mass="98098" checksum="250727A37F641D82" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZYB8</accession>
    <name>A0AA38ZYB8_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_009408</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZYB8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9697566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="117" mass="13290" checksum="7D550F316FA580DC" modified="2024-01-24" version="1">MTSQISVEVVHDPSSRSVHTTSGVGRFVGRNHSPGQLVIASPGTPLLEILVAKDSDEKRHENFSKNFHESCTCFIEYAETDYLRDCALYLYDKMLQLALSYQFSSDMEKEFRIIHTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZZQ8</accession>
    <name>A0AA38ZZQ8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">P-type Ca(2+) transporter</fullName>
            <ecNumber evidence="3">7.2.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PVL29_007404</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>Ca(2+)(in) + ATP + H2O = Ca(2+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18105"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.10" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZZQ8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005388">
        <property type="term" value="F:P-type calcium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000021">
        <property type="entry name" value="calcium-transporting ATPase, endoplasmic reticulum-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000014">
        <property type="entry name" value="Calcium-transporting ATPase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000028">
        <property type="entry name" value="Calcium-transporting P-type ATPase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000077">
        <property type="entry name" value="ECA1 (ER-TYPE CA2+-ATPASE 1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000065">
        <property type="entry name" value="Sarcoplasmic/endoplasmic reticulum calcium ATPase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42861">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08282">
        <property type="entry name" value="Hydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-1278">Translocase</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="112"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="315"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="355"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="894"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="982"/>
            <end position="1001"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1013"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1041"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="18">
        <location>
            <begin position="55"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ9698301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1093" mass="119880" checksum="CCC73C59E470C0D5" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A0E2</accession>
    <name>A0AA39A0E2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">UV-damaged DNA-binding protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_007637</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat DDB2/WDR76 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A0E2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080008">
        <property type="term" value="C:Cul4-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009411">
        <property type="term" value="P:response to UV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000408">
        <property type="entry name" value="protein DAMAGED DNA-BINDING 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033312">
        <property type="entry name" value="DDB2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15169">
        <property type="entry name" value="DAMAGE-SPECIFIC DNA BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15169:SF0">
        <property type="entry name" value="DNA DAMAGE-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="3 14" id="KW-0853">WD repeat</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="167"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="353"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="518"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="27"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="51"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00079162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9698670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="559" mass="62710" checksum="45F638B630513557" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A0U4</accession>
    <name>A0AA39A0U4_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_007697</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A0U4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32108">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32108:SF9">
        <property type="entry name" value="REVERSE TRANSCRIPTASE RNASE H-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9698762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="206" mass="23181" checksum="FE425FD4522639E1" modified="2024-01-24" version="1">MVLRNLQPRFARRLVGVPFQDLKSLVHAAFNVEEAITRGLWTDTTPSPDNKGKKPVGSYSRSGEYQYQPVYVQEPYIAQTSMQPRPPHPRATTHPPPRPYAQRPARQFTPLGMTLTRAFEKLRDASLIVPLAPRPLPHPIPPHFRSHEHCLYHQIQGHDTERCLALYHMIQDLIDSGLVNLAGPSVTTNPLPTHSTHAVPPPPSLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A1I0</accession>
    <name>A0AA39A1I0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Tr-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_007326</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A1I0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01883">
        <property type="entry name" value="EF1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03693">
        <property type="entry name" value="EF1_alpha_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03705">
        <property type="entry name" value="EF1_alpha_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000003">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000005">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000255">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00118_A">
        <property type="entry name" value="EF_Tu_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039297">
        <property type="entry name" value="COX7a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054696">
        <property type="entry name" value="GTP-eEF1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050100">
        <property type="entry name" value="TRAFAC_GTPase_members"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004539">
        <property type="entry name" value="Transl_elong_EF1A_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00483">
        <property type="entry name" value="EF-1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008969">
        <property type="entry name" value="PRK12317.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23115">
        <property type="entry name" value="TRANSLATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02238">
        <property type="entry name" value="COX7a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22594">
        <property type="entry name" value="GTP-eEF1A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="14">
        <location>
            <begin position="153"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9698189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="595" mass="66322" checksum="12523E3C60D1D70E" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A1Z5</accession>
    <name>A0AA39A1Z5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Nodulin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_008081</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9699304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A1Z5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd17354">
        <property type="entry name" value="MFS_Mch1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000446">
        <property type="entry name" value="Nodulin family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056555">
        <property type="entry name" value="NFD4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010658">
        <property type="entry name" value="Nodulin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21576:SF29">
        <property type="entry name" value="NODULIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21576">
        <property type="entry name" value="UNCHARACTERIZED NODULIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23262">
        <property type="entry name" value="NFD4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06813">
        <property type="entry name" value="Nodulin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="33"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="97"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="164"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="192"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="230"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="257"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="345"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="384"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="423"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="448"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="539"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="domain" description="Nodulin-like" evidence="7">
        <location>
            <begin position="32"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="NFD4 C-terminal" evidence="8">
        <location>
            <begin position="355"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="292"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="580"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="292"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="580"/>
            <end position="591"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF06813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9699304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="599" mass="65651" checksum="26E4DB82333B5E8D" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A2E3</accession>
    <name>A0AA39A2E3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Bet v I/Major latex protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_005526</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the BetVI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9699696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A2E3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010427">
        <property type="term" value="F:abscisic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009738">
        <property type="term" value="P:abscisic acid-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07816">
        <property type="entry name" value="Bet_v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.530.20:FF:000007">
        <property type="entry name" value="Major pollen allergen Bet v 1-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000916">
        <property type="entry name" value="Bet_v_I/MLP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024949">
        <property type="entry name" value="Bet_v_I_allergen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050279">
        <property type="entry name" value="Plant_def-hormone_signal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31213">
        <property type="entry name" value="OS08G0374000 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31213:SF55">
        <property type="entry name" value="STRESS-INDUCED PROTEIN SAM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00407">
        <property type="entry name" value="Bet_v_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00634">
        <property type="entry name" value="BETALLERGEN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01037">
        <property type="entry name" value="Bet_v_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00451">
        <property type="entry name" value="PATHOGENESIS_BETVI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0568">Pathogenesis-related protein</keyword>
    <keyword evidence="2 4" id="KW-0611">Plant defense</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bet v I/Major latex protein" evidence="5">
        <location>
            <begin position="1"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9699696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="159" mass="17275" checksum="3899E03C934E98EC" modified="2024-01-24" version="1">MGVFTYESEVTSSIPPAKMFKAAILDSDNLIPKVRPQAIKSVEIIQGKGGPGTIKKIHFGEGSKFKSMTHRVDAIDKENFTFSYTVVDGDVLTGGIESISHELKVVASPDGGSIYKNTSKYHTKSDVEISEEHIKGGKEEALALFKAIEAYVLAHPDAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A2S2</accession>
    <name>A0AA39A2S2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Lysosomal Pro-X carboxypeptidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_008118</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S28 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9699357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A2S2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008239">
        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070008">
        <property type="term" value="F:serine-type exopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.980:FF:000006">
        <property type="entry name" value="Serine carboxypeptidase S28 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.980">
        <property type="entry name" value="Serine carboxypeptidase S28, SKS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008758">
        <property type="entry name" value="Peptidase_S28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042269">
        <property type="entry name" value="Ser_carbopepase_S28_SKS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11010:SF96">
        <property type="entry name" value="LYSOSOMAL PRO-X CARBOXYPEPTIDASE-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11010">
        <property type="entry name" value="PROTEASE S28 PRO-X CARBOXYPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05577">
        <property type="entry name" value="Peptidase_S28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9699357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="506" mass="57094" checksum="250E8DD3534584A8" modified="2024-01-24" version="1">MNSSSLKVLLQWPISILAFVIFFSTTSTCVSATQPHLQRLSVRGRTFLREPETFSESVSEEFQTFYYNQTLDHFNYRPESYSTFQQRYVVNFKYWGGANASAPIFAYLGAEGDLDDDLPTIGFLTDNAHQFKALLVYIEHRYYGKSIPFGSREEALKNASIRGYFNSAQAIADYAEVLIHVKKKFFAENSPVIVVGASYGGMLASWFRLKYPHVALGALASSAPILYFEDITPQNGYYSIVTKDFREASESCYKTIRESWSEIDRVASEPNGLSILSTKFRTCDQLNNSDELKDYLDTIYCTAAQYNDPPMYPVTMACGGIDGAPEGSDILSRIFAGVVAFRGNRSCYHTKTNYFSIETIEGWAWQRCSEMVMPIGRGDNDTMFPPAPFNLTTFIRDCTSFYGVSPRPHWVTTYYGGHNIELILRRFASNIIFSNGLRDPYSSGGVLKNISDSVLGIFTVNGSHCLDILPTKSTDPEWLVTQRKAEVEIIESWIAQYYADLHAITK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A578</accession>
    <name>A0AA39A578_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_006334</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A578"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052258">
        <property type="entry name" value="Diverse_Func_Domain-Protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37612">
        <property type="entry name" value="FIBROIN HEAVY CHAIN FIB-H LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37612:SF11">
        <property type="entry name" value="GLYCINE-RICH CELL WALL STRUCTURAL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041392619" evidence="2">
        <location>
            <begin position="24"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9700954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="164" mass="14260" checksum="23D8F4512D2DEFD2" modified="2024-01-24" version="1">MAGLRSLALVALLVSSFVVVSQSRVARKDLGVDRGGVGVGVGVGVGVGLGLGGSGSGSGSGSGSGSGASSGAGSAASSGSGSGSGSEAGSYAGSRAGSGSGSNQGRGSGSGSGQGHGQGSGSGSGSGSGHGEGYGEGSGRGSGSGEGSGHGEGYGSGEGSGRGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A5K0</accession>
    <name>A0AA39A5K0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_006257</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A5K0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF78">
        <property type="entry name" value="OS12G0587133 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="169"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9700842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="902" mass="103811" checksum="76B8F42B63FEA49B" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A734</accession>
    <name>A0AA39A734_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_003939</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">The B regulatory subunit may modulate substrate selectivity and catalytic activity, and may also direct the localization of the catalytic enzyme to a particular subcellular compartment.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">PP2A consists of a common heteromeric enzyme, composed of a catalytic subunit (subunits C), a constant regulatory subunit (subunit A), and a variety of regulatory subunits such as subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families). Interacts with SIC/RON3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the phosphatase 2A regulatory subunit B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9701944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A734"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000569">
        <property type="entry name" value="Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000609">
        <property type="entry name" value="Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000009">
        <property type="entry name" value="PP2A_PR55"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018067">
        <property type="entry name" value="PP2A_PR55_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11871">
        <property type="entry name" value="PROTEIN PHOSPHATASE PP2A REGULATORY SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037309">
        <property type="entry name" value="PP2A_PR55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00600">
        <property type="entry name" value="PP2APR55"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01024">
        <property type="entry name" value="PR55_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01025">
        <property type="entry name" value="PR55_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 6" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="70"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="445"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="453"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00062838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU331113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9701944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="514" mass="57634" checksum="0DEC50066EC291C8" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A8H3</accession>
    <name>A0AA39A8H3_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_004621</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A8H3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35107">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35107:SF2">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041282070" evidence="2">
        <location>
            <begin position="28"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="112"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9702948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="186" mass="20192" checksum="EF55754E20D7F1B3" modified="2024-01-24" version="1">MAPLTQSVLSLLSAALLVGFLSAPAAARPCKTLFISSYSVSFHPNFPDQNNPNSAGTAGILTIFTEIRQFNPRPPLTFVDVAEDQRPIERPPRFGLGLGFDSDSLRDRTKDVLSVVVALLFGVGCGALTAATMYLGWSLFSNRHRYSDFDYDDFEDDDVDMSPKKVGYVKIPTPADASTPVAKEAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A9Q4</accession>
    <name>A0AA39A9Q4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TPX2 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_004723</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TPX2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A9Q4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005880">
        <property type="term" value="C:nuclear microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030295">
        <property type="term" value="F:protein kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090307">
        <property type="term" value="P:mitotic spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060236">
        <property type="term" value="P:regulation of mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027329">
        <property type="entry name" value="TPX2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009675">
        <property type="entry name" value="TPX2_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14326">
        <property type="entry name" value="TARGETING PROTEIN FOR XKLP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14326:SF39">
        <property type="entry name" value="TPX2 (TARGETING PROTEIN FOR XKLP2) PROTEIN FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06886">
        <property type="entry name" value="TPX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TPX2 C-terminal" evidence="6">
        <location>
            <begin position="76"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF06886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9703070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="188" mass="22115" checksum="1710E86BACF285CD" modified="2024-01-24" version="1">MESSHTRAAQKLVKASSQSAVPWSSTNRGVVRYNIPAQQGFRSHSWSKDYLKDQLGDKSKVFQKPPLKENTKPVEFKLHTQQRAVKRAMFNYSVATKLYFMEQQKKLEERLQKLIEEEEVRMLRKEMIPRAQLMPFFDRPFYPQRSNRPLTVPREPCFHNISSKCWSCISCSGLYDFHQQCPAMKPIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A9Z0</accession>
    <name>A0AA39A9Z0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_005136</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A9Z0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11446">
        <property type="entry name" value="bHLH_AtILR3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044579">
        <property type="entry name" value="bHLH11/121"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057075">
        <property type="entry name" value="bHLH_IRO3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47001:SF1">
        <property type="entry name" value="TRANSCRIPTION FACTOR BHLH11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47001">
        <property type="entry name" value="TRANSCRIPTION FACTOR BHLH121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23177">
        <property type="entry name" value="bHLH_IRO3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="8">
        <location>
            <begin position="50"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="205"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="90"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="34"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="205"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="236"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="281"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="300"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9703716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="327" mass="36189" checksum="857C98C97FB77D64" modified="2024-01-24" version="1">MMDQWKADDFTQSIAPELGPPNPLSLPSSNNRLPEARQRTEVEVKDSIAARKVQKADREKLRRDRLNEHFLELGNTLDPERPKNDKATILADTIQMLKDLTAEVNRLKVECAALSEESRELVQEKNELREEKVALKSDIDNLNVQYQQRLRVMFPWAPIDPSVVMGPSPYSYPVPVPVPSGPIPMHPSLQPFPFFGNQNPSGIPNPCSTFIPYQTPANPPTEVPSAQYASASHVSSKPDSKSKSSDRERSGNTERCDESNDVATDLELKTPGSSSQQDLLTGEKKGKQSQRKERSATDGSCSSRYSSSQGLQDSSSNSVGDLPKSDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AAQ1</accession>
    <name>A0AA39AAQ1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_005064</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AAQ1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35110">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35110:SF3">
        <property type="entry name" value="OS08G0360000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9703572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="121" mass="14059" checksum="18C0EDBF7ABE4BED" modified="2024-01-24" version="1">MFATAIRYLAKKPKPKQKPIELKTPPEQTKTLTRVIFDIVKEHGPLTIADTWEHVQQVGLRGLTSKRHMKIVLRWMRERQKLRLICNHVGPHKQFLYTTWFAKSNVNQPKPANGSSLPKLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AEA3</accession>
    <name>A0AA39AEA3_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_003859</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AEA3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34287:SF4">
        <property type="entry name" value="OS04G0504200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34287">
        <property type="entry name" value="OS06G0551500 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="145"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9705947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="172" mass="19596" checksum="C889BE490D804445" modified="2024-01-24" version="1">MSSALAVAVAVAVPEEEEEEEGRLIKVVEYVESSLCEELLCKFPDTSPFDFDYSQSSIWSPLVPRPQKSLALNWDLGTPPPKLSYRLGLDKTSLKKVTANLKNNIFTMFKKKQKKRRRSFIKPSDHFSPTPPSTKGWSRVLKAASKHFKKKKKKDPTNLHVKLSNYLRQSDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AES8</accession>
    <name>A0AA39AES8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_003230</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AES8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000381">
        <property type="entry name" value="Putative receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000420">
        <property type="entry name" value="Putative receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043891">
        <property type="entry name" value="SPARK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47989">
        <property type="entry name" value="OS01G0750732 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47989:SF36">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19160">
        <property type="entry name" value="SPARK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041356663" description="Protein kinase domain-containing protein" evidence="9">
        <location>
            <begin position="26"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="250"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="325"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="608"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="608"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="353"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9705073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="673" mass="74179" checksum="2DECB94C62634970" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AJH5</accession>
    <name>A0AA39AJH5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF868 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_000681</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AJH5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008586">
        <property type="entry name" value="DUF868_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31972:SF11">
        <property type="entry name" value="DUF868 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31972">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05910">
        <property type="entry name" value="DUF868"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9708783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="300" mass="34108" checksum="00F5E006169829A1" modified="2024-01-24" version="1">MHEAIGIPACFSSGERAVDDQAAVTRSSQSVFMSVYRTKIAGQCRLITITWCKDLLLHGLSVSVQGPDGDSHYQSKVELKPWYFWRKQGSKHFTVDGKTVDVVWDLKAAKFNGETEPRSDYYVAIVCEEEVVLLLGDLKKDAYRKTGCRPALIEPTLVSRKEHLFGKKKFSTKVKFHEKGNFHEISIECNNSLNKNSSAGEFDHEMEIRIDGRLANHVKHLQWKFRGNESVIVNKTRVEVYWDVHDWLFSSGPRQGLFIFKPISSSTLLSTQIADSTAGEECSEGRSSKFCLFLYAWKVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AJQ9</accession>
    <name>A0AA39AJQ9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nucleotide-diphospho-sugar transferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_002647</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AJQ9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052636">
        <property type="term" value="F:arabinosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052325">
        <property type="term" value="P:cell wall pectin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053250">
        <property type="entry name" value="Glycosyltransferase_77"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005069">
        <property type="entry name" value="Nucl-diP-sugar_transferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46936">
        <property type="entry name" value="ARABINOSYLTRANSFERASE XEG113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46936:SF1">
        <property type="entry name" value="ARABINOSYLTRANSFERASE XEG113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03407">
        <property type="entry name" value="Nucleotid_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="15"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleotide-diphospho-sugar transferase" evidence="3">
        <location>
            <begin position="152"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9707698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="626" mass="71436" checksum="F1F813A1F83D6CA2" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AJT5</accession>
    <name>A0AA39AJT5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Beta-glucuronosyltransferase GlcAT14A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_000413</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AJT5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015020">
        <property type="term" value="F:glucuronosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044610">
        <property type="entry name" value="GLCAT14A/B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003406">
        <property type="entry name" value="Glyco_trans_14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45719:SF14">
        <property type="entry name" value="BETA-GLUCURONOSYLTRANSFERASE GLCAT14A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45719">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02485">
        <property type="entry name" value="Branch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9708357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="403" mass="45905" checksum="E913B9222DF4EDB5" modified="2024-01-24" version="1">MAMVAEKKWLFTLFSAAFVSLLLFLSSICGFNASYALSFHKPFSSTVHHGLHYPPAFAYYISGGRGHKDRILRLLLAVYHPRNRYLLHLGADASDEERRLLASAVKSVPAIRAFGNVDVVGKPDRLTYMGSSNIAATLRAASILLRVDSGWNWFITLSAMDYPLITQDDLSHVFSSVRRDLNFIDHTSDLGWKESQRVHPIVVDPSIYLARRSQIFHATEKRPTPDSFKFFTGSPWVILNRSFLEFCILGWDNLPRTLLMYFTNVILSDEGYFHSVICNSPEFRNTTVNNDLRYVIWDSPPKMEPHFLNVSDYDQTVQSGAAFARQFKKDDPVLNMIDEKILKRGRNRVVPGAWCTGRKSWWMDPCSNWGDANVLRPGPQARKLEESVTNLLEDSNSQSNQCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AKK3</accession>
    <name>A0AA39AKK3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">P-loop containing nucleoside triphosphate hydrolases superfamily protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_000252</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the arsA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AKK3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02035">
        <property type="entry name" value="ArsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025723">
        <property type="entry name" value="ArsA/GET3_ATPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053262">
        <property type="entry name" value="ArsA_ATPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040612">
        <property type="entry name" value="ArsA_HSP20-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43868">
        <property type="entry name" value="OS02G0711200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43868:SF1">
        <property type="entry name" value="P-LOOP CONTAINING NUCLEOSIDE TRIPHOSPHATE HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02374">
        <property type="entry name" value="ArsA_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17886">
        <property type="entry name" value="ArsA_HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ArsA/GET3 Anion-transporting ATPase-like" evidence="3">
        <location>
            <begin position="42"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="ArsA HSP20-like" evidence="4">
        <location>
            <begin position="382"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="354"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF17886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9708103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="443" mass="47935" checksum="F79B66A7CF14922D" modified="2024-01-24" version="1">MASTSLLLSSASSRNPNSELSIRRRRHGALATTASSEEDHSTKLVTFLGKGGSGKTTSAIFAAQHYAMAGFNTCLVIHSQDPTAEYLLNCKIGTSPIICNNNLSAVRLETTKMLLEPLHLLKKADAELNITQGVLEGVVGEELGVLPGMDSAFTLLALERLVGFLGNLGRRNLQKDKYDIIIYDGINTEETLRMIGVTSRARLYLKYLRNMAERTDIGRLAGPSLLRLVDEAMSLSTRGSNLNGKMSAEIWDILERAIERGSSAFGEPRKFGCYLVVDPNNPASVSSALRYWGCAIQAGAQVSGAFGTASPHSDVESVEIVKNFSPLPFALCPHVPMGSLPDWNAIISSNPSEDARDLLSAPGSSSSSSNVMEPVKFDPSKKSVSLLMPGFDKSEIKLYQYRGGSELLVEAGDQRRVIRLPPEIQGRVGGAKFADRKLVITMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AN58</accession>
    <name>A0AA39AN58_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">TIR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_000887</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the disease resistance TIR-NB-LRR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050832">
        <property type="term" value="P:defense response to fungus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043068">
        <property type="term" value="P:positive regulation of programmed cell death"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10140:FF:000007">
        <property type="entry name" value="Disease resistance protein (TIR-NBS-LRR class)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058192">
        <property type="entry name" value="WHD_ROQ1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017:SF385">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN (TIR-NBS-LRR CLASS)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23282">
        <property type="entry name" value="WHD_ROQ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0611">Plant defense</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="TIR" evidence="10">
        <location>
            <begin position="19"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9709139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1399" mass="157288" checksum="5C506EEB540C859A" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AQK8</accession>
    <name>A0AA39AQK8_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_001235</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43939:SF68">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 290 KDA-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43939">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 158"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="228"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="351"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="561"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="716"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="940"/>
            <end position="1170"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1347"/>
            <end position="1374"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1466"/>
            <end position="1538"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1596"/>
            <end position="1654"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9709649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1864" mass="209058" checksum="D6EA3C9B2EBC5A9F" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D6M2</accession>
    <name>A0AA39D6M2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_025568</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D6M2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11446">
        <property type="entry name" value="bHLH_AtILR3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14686">
        <property type="entry name" value="bZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000104">
        <property type="entry name" value="Transcription factor bHLH34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044818">
        <property type="entry name" value="ILR3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46133">
        <property type="entry name" value="BHLH TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46133:SF1">
        <property type="entry name" value="TRANSCRIPTION FACTOR ILR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="7">
        <location>
            <begin position="145"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="193"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9671980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="306" mass="33594" checksum="2D3BB483D2B9D34F" modified="2024-01-24" version="1">MGHTAQKPNLRISTLAICLVANYPTFFHTRIPFSPSRKSPFSLSPNTLTLLLLPGNCRRASSIFGDSFEMVSPEATNWLYEYGLIEDIPVPDSNFGNTNSGFAWTPVQALNTSANVSVEIDGSFGDSDGIKETGSKKRVRSESCGASSSKACREKLRRDRLNDKFMELGSILEPGRPPKTDKSSILIDAVRMVSQLRGESQKLKDSNSSLQEKIKELKAEKNELRDEKQRLKAEKEKLEQQLKAMNAQPSFLPPVPSIPAAFAAQGQAGGNKLVPFIGYPGVAMWQFMPPAAVDTSQDHVLRPPVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D750</accession>
    <name>A0AA39D750_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_025418</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D750"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050511">
        <property type="entry name" value="AMPK_gamma/SDS23_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780">
        <property type="entry name" value="AMP-ACTIVATED PROTEIN KINASE, GAMMA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780:SF128">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0129">CBS domain</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CBS" evidence="5">
        <location>
            <begin position="334"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="298"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9671692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="394" mass="42519" checksum="96ECEFA87DC1AD09" modified="2024-01-24" version="1">MAFSLLAHVVSDLCLGKPALRSLSISATVGEALSALKTSEDSFISVWSCDHSANIQDECRCVGKICMVDVVCYLCTDDNLLSPSSALKSPVSDLLPNIPGLVMHVEPHSSLLEAIDLILQGAQNLVVPIRSSISNSSRRKLYQKPQTSPTTMHKGCEYCWLTQEDVVRYLLSSIGLLSPIAALPIDTLRIIDTDVLAINYHSPASSSLPAILRSLRDQTSVAVVDENGGLIGEISPFTLACCDETVAAAITTLSSGDLMAYIDCGGPPEEIVRMVKTRLKQRNLEGMLEEFALDSSSTLSLSSSSSDEESLPSPKTALSRSGKYSRSSSYSARMVRRAEAIVCHPGSSLVAVMIQAIAHRVNYVWVIEDDCCLAGIVTFSSMLKIFREHLESMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D9G5</accession>
    <name>A0AA39D9G5_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_024170</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D9G5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33133">
        <property type="entry name" value="OS08G0107100 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33133:SF51">
        <property type="entry name" value="THH1_TOM1_TOM3 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9675115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="152" mass="17600" checksum="A34EE0C3123496BD" modified="2024-01-24" version="1">MVTFLSVFVAFFAYNIVAKQILIVWVVGIRPVKIGILINFFVVWQLAIMISALEDLYGFRVMVKSRALISGYLQMAMVILLKLNFSLFVIQMAFERLVVHEASLGMAYAILCFLLLLKLFLFRLSYRHENIDKATLFDHLEVYMGEYGKIFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DAM6</accession>
    <name>A0AA39DAM6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF4283 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_025131</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9676450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DAM6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025558">
        <property type="entry name" value="DUF4283"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34427">
        <property type="entry name" value="DUF4283 DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34427:SF5">
        <property type="entry name" value="DUF4283 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14111">
        <property type="entry name" value="DUF4283"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4283" evidence="1">
        <location>
            <begin position="156"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9676450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="568" mass="63133" checksum="D9CA95D2F8EEED51" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DBG2</accession>
    <name>A0AA39DBG2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_022389</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DBG2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040008">
        <property type="term" value="P:regulation of growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11442">
        <property type="entry name" value="bHLH_AtPRE_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000082">
        <property type="entry name" value="Transcription factor ILI6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044293">
        <property type="entry name" value="PRE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46446">
        <property type="entry name" value="TRANSCRIPTION FACTOR PRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46446:SF3">
        <property type="entry name" value="TRANSCRIPTION FACTOR PRE3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23174">
        <property type="entry name" value="bHLH_ILI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0341">Growth regulation</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="7">
        <location>
            <begin position="5"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="49"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9677380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="91" mass="10219" checksum="9470F39A1448F921" modified="2024-01-24" version="1">MSSRRSRSRQSGSSRITDDQINDLVSKLQQLLPEIRGRQSDKVSAAKVLQETCNYIRSLNREVDDLSERLSELLATTDSAQAAIIRSLLTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DBV1</accession>
    <name>A0AA39DBV1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">MADS-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_022824</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9678054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DBV1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000987">
        <property type="term" value="F:cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00266">
        <property type="entry name" value="MADS_SRF_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050142">
        <property type="entry name" value="MADS-box/MEF2_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033897">
        <property type="entry name" value="SRF-like_MADS-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48019">
        <property type="entry name" value="SERUM RESPONSE FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00404">
        <property type="entry name" value="MADSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="7">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="94"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9678054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="331" mass="37975" checksum="71091D1774DBD3CE" modified="2024-01-24" version="1">MARGKLKMELIANEKLRCRTFRNRQKGLKKKLHELSTLCDVEACMIIYCDQNGNGTYSSQPDVWPENHYEVQRIVNKYISERKEDHGKRTVALSDILESRKRKAELELQKLQEKNGKTKGQTSETRLELDGLSYEKLMEINDKLDRKLEHVKSLIDLKKGQAGLMSKTPVNSSHIPGLPTAEQAFQGTKMVLYDLDFPDTSSGVVESYLNRMMMNMENNGYPEFGSGKASSSELLHHSTQFQDPIHCDPPHGMFKNNTDPSTSYQKMGTVPTKQEMSVSKNTMMFNTHPTNSMPQMGLSENMMFDMDHSIFMQQISAYRQYLLDGYLFFHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DDM0</accession>
    <name>A0AA39DDM0_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_021690</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SPIRAL1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DDM0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010005">
        <property type="term" value="C:cortical microtubule, transverse to long axis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043622">
        <property type="term" value="P:cortical microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039613">
        <property type="entry name" value="SPR1/2/3/4/5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33403:SF48">
        <property type="entry name" value="PROTEIN SPIRAL1-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33403">
        <property type="entry name" value="SPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0493">Microtubule</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="93"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="98"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9679855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="118" mass="11449" checksum="F60BF2008174A6E0" modified="2024-01-24" version="1">MGRGVSSGGGQSSLGYLFGSGEAPKPASNNAPVPPSEAPAVNNGPSPKPTTPAQPADVTKQIPAGIHGNLANNYYRADGQNCGNFITDRPSTKVHAAPGGGSSLGYLFGGGGGGGSGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DDY0</accession>
    <name>A0AA39DDY0_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_019473</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DDY0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053258">
        <property type="entry name" value="Ca-permeable_cation_channel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34115">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34115:SF5">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9680185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="200" mass="22498" checksum="A024A35E075A1424" modified="2024-01-24" version="1">MPIHIATLFTFLGIHLPQQQNRMATTRSAILAILTLVVPLLLDLIQLKFQNESISAFEAHPITTKVAIAALQLFALSFTLEEYFAHLSPTPAALLRTSMVFSGSLLVASLVSILSSDGWRPVIYVIYVFLSLGNYLYRLVRKGCERVQRIMEKLQTLFARMQQRPVRRNLAILPLSSMDTRHRVRIDTSRSTASTSLVIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DFW4</accession>
    <name>A0AA39DFW4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NB-ARC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_018379</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DFW4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050905">
        <property type="entry name" value="Plant_NBS-LRR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463:SF198">
        <property type="entry name" value="RPP4C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0611">Plant defense</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NB-ARC" evidence="4">
        <location>
            <begin position="68"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="4">
        <location>
            <begin position="146"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9682449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="258" mass="28723" checksum="28FD3927D7AA6381" modified="2024-01-24" version="1">MEEAGKFLEDGKKANKSCFMGLCPNLKLEVVEIQGARKFERLSYRAPLPGIGSATLRGYKALESRMSTLNQIMEALRDGDDNMIGVWGMGGVGKTTLAEQVAKQAKEHKLFDEVVMASVFQNPDLRKIQGQLADMVGLKFEEETQLDLEEVRIPFGDDHKRCKIVLTSRNKHVLSNEMGTQKDIPVLHLTEKEALVLFKKIVGDSIDKQDLQAIVINVAKECKGLPIALVIVAKALKNKSVYMEECFTTVKKGLCQQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DG70</accession>
    <name>A0AA39DG70_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_018421</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DG70"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9682495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="83" mass="8798" checksum="93D49CB599386011" modified="2024-01-24" version="1">MPPSRTPSPTVLAAMYSEVQTSRLNRSLPLPSVLKSLFKIVYSPLSSVTGNPGEIADEPTSSSYKKLKVGIALSSGQASKDTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DGB5</accession>
    <name>A0AA39DGB5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ascorbate ferrireductase (transmembrane)</fullName>
            <ecNumber evidence="11">7.2.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_018796</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>Fe(3+)(out) + L-ascorbate(in) = monodehydro-L-ascorbate radical(in) + Fe(2+)(out) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:30403"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
            <dbReference type="ChEBI" id="CHEBI:59513"/>
            <dbReference type="EC" id="7.2.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.2.1.3" evidence="11"/>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DGB5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140571">
        <property type="term" value="F:transmembrane ascorbate ferrireductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd08766">
        <property type="entry name" value="Cyt_b561_ACYB-1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1770:FF:000001">
        <property type="entry name" value="Cytochrome b reductase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043205">
        <property type="entry name" value="CYB561/CYBRD1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006593">
        <property type="entry name" value="Cyt_b561/ferric_Rdtase_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10106">
        <property type="entry name" value="CYTOCHROME B561-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10106:SF0">
        <property type="entry name" value="LD36721P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03188">
        <property type="entry name" value="Cytochrom_B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00665">
        <property type="entry name" value="B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50939">
        <property type="entry name" value="CYTOCHROME_B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4" id="KW-0349">Heme</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="46"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="84"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="114"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="147"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="186"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="229"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b561" evidence="14">
        <location>
            <begin position="46"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00051575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9682946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="265" mass="28982" checksum="88D8DCCD1E4ED8C6" modified="2024-01-24" version="1">MNPSTSQSHTPIPVQEDALSLLPVSKVLSLDSTMAVPVVRFPIIRLVRFIGVVVTAFVLTWAVHYRGGMALISENKDLIFNVHPVLMVIGLILLNGEAMLAYKTVSGTKNFKKLVHLSLQFLAFCLSLVGVWAALKFHNERGIDNFYSLHSWLGLACLFLFGIQWATGFATFWYPGGSRNSRASLLPWHVFFGVYIYALAVATATTGILEKATFLQTNKVISRYSMEALLVNSLGILIVVLGGFVILAVVTPANGKGDIPRGSIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DGJ5</accession>
    <name>A0AA39DGJ5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_018995</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DGJ5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021775">
        <property type="entry name" value="DUF3339"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33128:SF66">
        <property type="entry name" value="DUF3339 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33128">
        <property type="entry name" value="OS05G0103400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11820">
        <property type="entry name" value="DUF3339"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9683231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="69" mass="7706" checksum="195B0EE01E81370B" modified="2024-01-24" version="1">MSDWGAVFVAVMLFILLMPGLLIQIPGRGRFIEFTNFQTSGVSILVHSLIYFTLICIFLLAIGVHMYIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DJ43</accession>
    <name>A0AA39DJ43_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_017498</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DJ43"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF78">
        <property type="entry name" value="OS12G0587133 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="463"/>
            <end position="743"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9685480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1196" mass="138138" checksum="632F802D87773B09" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DNZ5</accession>
    <name>A0AA39DNZ5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Katanin p80 WD40 repeat-containing subunit B1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_013836</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">May participate in a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cytoplasm</location>
            <location evidence="1 7">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the WD repeat KATNB1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DNZ5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008352">
        <property type="term" value="C:katanin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007019">
        <property type="term" value="P:microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051013">
        <property type="term" value="P:microtubule severing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000192">
        <property type="entry name" value="Katanin p80 WD40 repeat-containing subunit B1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002029">
        <property type="entry name" value="Katanin p80 WD40 repeat-containing subunit B1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03022">
        <property type="entry name" value="Katanin_p80_B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028021">
        <property type="entry name" value="Katanin_C-terminal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026962">
        <property type="entry name" value="KTNB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19845">
        <property type="entry name" value="KATANIN P80 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19845:SF0">
        <property type="entry name" value="KATANIN P80 WD40 REPEAT-CONTAINING SUBUNIT B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13925">
        <property type="entry name" value="Katanin_con80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4 7" id="KW-0493">Microtubule</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 8" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="12"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="55"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="97"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="139"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="181"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Katanin p80 subunit C-terminal" evidence="10">
        <location>
            <begin position="561"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="359"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="448"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="376"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="470"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9691763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="722" mass="79061" checksum="FEB784BCD473EA26" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DPH8</accession>
    <name>A0AA39DPH8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Xyloglucan endotransglucosylase/hydrolase</fullName>
            <ecNumber evidence="12">2.4.1.207</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_014939</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Secreted</location>
            <location evidence="12">Cell wall</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Secreted</location>
            <location evidence="12">Extracellular space</location>
            <location evidence="12">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="12">Contains at least one intrachain disulfide bond essential for its enzymatic activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the glycosyl hydrolase 16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.207" evidence="12"/>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9689497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DPH8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016762">
        <property type="term" value="F:xyloglucan:xyloglucosyl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042546">
        <property type="term" value="P:cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010411">
        <property type="term" value="P:xyloglucan metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02176">
        <property type="entry name" value="GH16_XET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000025">
        <property type="entry name" value="Xyloglucan endotransglucosylase/hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044791">
        <property type="entry name" value="Beta-glucanase/XTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008263">
        <property type="entry name" value="GH16_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010713">
        <property type="entry name" value="XET_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016455">
        <property type="entry name" value="XTH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31062">
        <property type="entry name" value="XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASE PROTEIN 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00722">
        <property type="entry name" value="Glyco_hydro_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06955">
        <property type="entry name" value="XET_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005604">
        <property type="entry name" value="XET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01034">
        <property type="entry name" value="GH16_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 12" id="KW-0052">Apoplast</keyword>
    <keyword evidence="1 12" id="KW-0134">Cell wall</keyword>
    <keyword evidence="12" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 12" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 12" id="KW-0732">Signal</keyword>
    <keyword evidence="4 12" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041488835" description="Xyloglucan endotransglucosylase/hydrolase" evidence="12">
        <location>
            <begin position="30"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="14">
        <location>
            <begin position="26"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="233"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="233"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="10">
        <location>
            <position position="106"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="110"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="11">
        <location>
            <position position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR005604-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR005604-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9689497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="297" mass="32979" checksum="66F5E39B5D41DE94" modified="2024-01-24" version="1">MAPIYSSSSAVSTALLISVVVSFQMAASAGNFNQDFDITWGDGRAKILNNGALLTLSLDKTSGSGFQSKNEYLFGKIDMQLKLVPGNSAGTVTAYYLSSQGSTHDEIDFEFLGNLSGDPYILHTNVFGQGKGNREQQFYLWFDPTADFHAYSILWNPQRIIFSVDGTPIREFKNSESIGVPYPKNQPMRLYSSLWNADDWATRGGLVKTDWTQAPFTASYRNFNADACIWSSGASSCSSNTPTSTSTSTDWYSQELDSTSQERMKWVQKNYMIYNYCTDTKRFPQGLPPECTATTTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DPV6</accession>
    <name>A0AA39DPV6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phospholipase/carboxylesterase/thioesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_012504</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily. AB hydrolase 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9689872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DPV6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008474">
        <property type="term" value="F:palmitoyl-(protein) hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000232">
        <property type="entry name" value="Probable carboxylesterase SOBER1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050565">
        <property type="entry name" value="LYPA1-2/EST-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003140">
        <property type="entry name" value="PLipase/COase/thioEstase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10655:SF17">
        <property type="entry name" value="LYSOPHOSPHOLIPASE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10655">
        <property type="entry name" value="LYSOPHOSPHOLIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02230">
        <property type="entry name" value="Abhydrolase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipase/carboxylesterase/thioesterase" evidence="6">
        <location>
            <begin position="37"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9689872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="253" mass="27504" checksum="DFB7D832FC0BD846" modified="2024-01-24" version="1">MDLITVSTKPIALLTITLGSTIFFILLLQSHTFSPKPDSMARSFVLWLHGLGDSGPANEPIKTLFTSPEFRNTIWKFPSAPSNPVTCNYGSVMPSWFDIHEIPVTADSPKDENGVLKAVQNVHAMIDKELAAGTNPKNIFVCGFSQGGALTLASVLLYPRTLGGGAVFSGWVPFNSTMIEQMPADAKKTPLLWSHGMADRTVLFEAGQAGPPFLEQAGVSCEFKAYPGLAHSISNEELRYLESWIKTRLQSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DQD8</accession>
    <name>A0AA39DQD8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">K Homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_011399</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DQD8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006307">
        <property type="term" value="P:DNA alkylation repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1140.10">
        <property type="entry name" value="Cyclic phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019510">
        <property type="entry name" value="AKAP7-like_phosphoesterase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009210">
        <property type="entry name" value="ASCC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009097">
        <property type="entry name" value="Cyclic_Pdiesterase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13360">
        <property type="entry name" value="ACTIVATING SIGNAL COINTEGRATOR 1 COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13360:SF1">
        <property type="entry name" value="ACTIVATING SIGNAL COINTEGRATOR 1 COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10469">
        <property type="entry name" value="AKAP7_NLS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55144">
        <property type="entry name" value="LigT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="3">
        <location>
            <begin position="156"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="264"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="269"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9692319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="514" mass="58014" checksum="54AB58CCCC475E72" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DRW4</accession>
    <name>A0AA39DRW4_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_012252</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9693400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DRW4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9693400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="100" mass="10763" checksum="AF22B95C68F3A660" modified="2024-01-24" version="1">MSPIEEGFSFEPKAIVAAGVGDVGLDGEFGPSAEQAFFEGERSIALEVISSFFSSSQIIGGTNWFTPTSTSSLEDEEDKEDEEDEADDKNEATRGKVLTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DV99</accession>
    <name>A0AA39DV99_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">L-ascorbate peroxidase</fullName>
            <ecNumber evidence="3">1.11.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_007365</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peroxidase family. Ascorbate peroxidase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.11" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DV99"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016688">
        <property type="term" value="F:L-ascorbate peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000302">
        <property type="term" value="P:response to reactive oxygen species"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00691">
        <property type="entry name" value="ascorbate_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.520.10:FF:000003">
        <property type="entry name" value="Cytosolic ascorbate peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.420.10:FF:000003">
        <property type="entry name" value="L-ascorbate peroxidase, cytosolic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.420.10">
        <property type="entry name" value="Peroxidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044831">
        <property type="entry name" value="Ccp1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002207">
        <property type="entry name" value="Peroxidase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019794">
        <property type="entry name" value="Peroxidases_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019793">
        <property type="entry name" value="Peroxidases_heam-ligand_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31356:SF59">
        <property type="entry name" value="L-ASCORBATE PEROXIDASE 1, CYTOSOLIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31356">
        <property type="entry name" value="THYLAKOID LUMENAL 29 KDA PROTEIN, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00459">
        <property type="entry name" value="ASPEROXIDASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00458">
        <property type="entry name" value="PEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00435">
        <property type="entry name" value="PEROXIDASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00436">
        <property type="entry name" value="PEROXIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50873">
        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0349">Heme</keyword>
    <keyword evidence="11" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="8" id="KW-0630">Potassium</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Plant heme peroxidase family profile" evidence="13">
        <location>
            <begin position="74"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="113"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9698243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="250" mass="27422" checksum="CFE8DB455FEC1694" modified="2024-01-24" version="1">MGKCYPTVSEEYKKAIEKAKKKLRGLIAEKNCAPIMLRIAWHSAGTFDVKTKTGGPFGTMKKPEELAHGANNGLDIAVRLLEPIKEQFPIISYADFYQLAGVVAVEVTGGPEVPFHPGREDKPNPPPEGRLPDATKGCDHLRQVFCSQMGLSDKDIVALSGGHTLGRCHKERSGFEGPWTSNPLIFDNSYFTILLSGEKEGLLQLPSDKALLSDPAFRPLVEKYAADEDAFFEDYSEAHLKLSELGFADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DWX1</accession>
    <name>A0AA39DWX1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_008969</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the mTERF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DWX1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.70.10:FF:000001">
        <property type="entry name" value="Mitochondrial transcription termination factor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.70.10">
        <property type="entry name" value="Transcription termination factor 3, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003690">
        <property type="entry name" value="MTERF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038538">
        <property type="entry name" value="MTERF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13068">
        <property type="entry name" value="CGI-12 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13068:SF133">
        <property type="entry name" value="MITOCHONDRIAL TRANSCRIPTION TERMINATION FACTOR FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02536">
        <property type="entry name" value="mTERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00733">
        <property type="entry name" value="Mterf"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0806">Transcription termination</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9696997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="389" mass="43696" checksum="73EFE1AA01B4FBB4" modified="2024-01-24" version="1">MSCIFLCERMWLSPSWRRIGGASMTQLHFLGNITPFVIRCFSSSKRHSFTVSYLINSCGLSPETAISTSKKVQFENPENPDSVLALLRNHGCTDAHISKIVAKHPLLLLANPEKTLLPKLQFLGSVGLSHVDLAKVLASTPSILRMSLEKTLIPTYNLLKGVVIGDENAAKALTKQCRISCGNVEKTVAPNATLLREIGVPMAHISFLVTNYPTLCQKRDKFSKTVKKVMEMGFDPQRLLFVNALQVICQMSESTWEQKINAYKRCGLSEDEIVLAFRNHPICFQLSEKKIMSTMDYLVNMGWQPASIARVPAVLFFNLERRIVPRCSVAKVLLLKGLVKKDLCLGTFLKLTDRAFIDRFIIKYQKYVPQLLDVYHGKVGFQELGLVSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DX04</accession>
    <name>A0AA39DX04_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TOG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_005723</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DX04"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005881">
        <property type="term" value="C:cytoplasmic microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024395">
        <property type="entry name" value="CLASP_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21567:SF65">
        <property type="entry name" value="ARM REPEAT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21567">
        <property type="entry name" value="CLASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12348">
        <property type="entry name" value="CLASP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TOG" evidence="2">
        <location>
            <begin position="61"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9700028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="297" mass="32799" checksum="2E04C8079E99045E" modified="2024-01-24" version="1">MALRSLDNALPTTPERPKKQAKVAVLTQKQSDFGVNDENKAPLPPTADATIDYISSENLQAMPDPETQITGLTEGLDSKDWTKVCESLNNARRFALYHSALMAPILEKVLLVLVKAMKNPRSALSKTSIMASTDIFNTFGDELLQPSNSDAFDHMLLQLLLKASQDKKFVCEEADKALKAMVGSMTPLPLLQKLQAYVNHANLRIRAKAAISISHCVSKMGLEGMKEFGLVSLVQIAAELLNDRLPEAREAARSTVISIYDAFTENEEQKQESWQNFCQSNLPPLHAQSMVKIISSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DX06</accession>
    <name>A0AA39DX06_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Myb/SANT-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_006349</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DX06"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045026">
        <property type="entry name" value="LIMYB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024752">
        <property type="entry name" value="Myb/SANT-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47584">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47584:SF17">
        <property type="entry name" value="MYB_SANT-LIKE DNA-BINDING DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12776">
        <property type="entry name" value="Myb_DNA-bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb/SANT-like" evidence="1">
        <location>
            <begin position="12"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9700976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="257" mass="30262" checksum="744E142E9A74A09E" modified="2024-01-24" version="1">MSQNVEISRANWTDPIQRKHFIDLCLQEANKGFRSGGGLKSSAWPQIAKEFEKLLGKRYTSKQLKNGWDYMKRQYLILSKTMTMTGHGYNSVTKTFDWPTEKWEEYLQKYPEAKQFHFKPLANVEELEALFGEVLAIGFKNWSSRGVIASRAEESSTHSIFMPSETSISLEEDEDFPRNTNNEQTQEEMNRIMNVLENFEGPLAKECIKILKRLLTYEDPLYYVAINSFCKKKEYREVWMEMESDEERMGWIQSLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DX35</accession>
    <name>A0AA39DX35_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">1-phosphatidylinositol 4-kinase</fullName>
            <ecNumber evidence="2">2.7.1.67</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_009100</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PI3/PI4-kinase family. Type II PI4K subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.67" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DX35"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004430">
        <property type="term" value="F:1-phosphatidylinositol 4-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044571">
        <property type="entry name" value="P4KG1-8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45800:SF11">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3-KINASE-RELATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45800">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-KINASE GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9697177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="147" mass="16604" checksum="5178B11D5B04809F" modified="2024-01-24" version="1">MVNFEAKTTIPSHLLKRNIHVHNDKQTKQSIIKGLCLPSLEMYPCLDAPGKTLGQPSLKRSVRVREKSFKEVAAYLLDYNHFANVPSTALVKITHSIFNANDRQFIPHDFDVGDHGTSSFTVSTVHRIGTLEIRISKHRNLSSQLLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DXN0</accession>
    <name>A0AA39DXN0_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_006137</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DXN0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9700673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="176" mass="17747" checksum="589B85EBF090B1E4" modified="2024-01-24" version="1">MGLVACAPGVVSLPGVGFEFCQMACVLEGASLPGVVCQMGLVACVRGVASLPGEGYEFCQMAFVLVAASLPGVVCQMGLVACVRGVASLPGEGYEFCQMAFVLVAASLPGVVCQMGLVACVRGAASLPGEGYEFCQMAFVLVAASLPGVVCQMGLVACVRELASLPCEVQLVAGVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DXS3</accession>
    <name>A0AA39DXS3_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_005687</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9699969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DXS3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015421">
        <property type="term" value="F:ABC-type oligopeptide transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010329">
        <property type="term" value="F:auxin efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010328">
        <property type="term" value="F:auxin influx transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090374">
        <property type="term" value="P:oligopeptide export from mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18577">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18578">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03249">
        <property type="entry name" value="ABC_MTABC3_MDL1_MDL2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000009">
        <property type="entry name" value="ABC transporter B family member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000066">
        <property type="entry name" value="ABC transporter B family member 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000044">
        <property type="entry name" value="ABC transporter B family member 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394:SF16">
        <property type="entry name" value="ABC TRANSPORTER B FAMILY MEMBER 4-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="69"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="120"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="193"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="218"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="296"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="705"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="747"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="833"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="929"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="14">
        <location>
            <begin position="73"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="13">
        <location>
            <begin position="395"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="14">
        <location>
            <begin position="707"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="13">
        <location>
            <begin position="1028"/>
            <end position="1265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9699969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1273" mass="138134" checksum="EBDE844E7203BA3E" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DYP2</accession>
    <name>A0AA39DYP2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Ferritin</fullName>
            <ecNumber evidence="14">1.16.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_007701</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation.</text>
    </comment>
    <comment type="function">
        <text evidence="14">Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 14">
            <text>4 Fe(2+) + O2 + 4 H(+) = 4 Fe(3+) + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:11148"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="1.16.3.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Oligomer of 24 subunits. There are two types of subunits: L (light) chain and H (heavy) chain. The major chain can be light or heavy, depending on the species and tissue type. The functional molecule forms a roughly spherical shell with a diameter of 12 nm and contains a central cavity into which the insoluble mineral iron core is deposited.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the ferritin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.16.3.1" evidence="14"/>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DYP2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008199">
        <property type="term" value="F:ferric iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004322">
        <property type="term" value="F:ferroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01056">
        <property type="entry name" value="Euk_Ferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1260.10:FF:000006">
        <property type="entry name" value="Ferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001519">
        <property type="entry name" value="Ferritin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009040">
        <property type="entry name" value="Ferritin-like_diiron"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014034">
        <property type="entry name" value="Ferritin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008331">
        <property type="entry name" value="Ferritin_DPS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11431">
        <property type="entry name" value="FERRITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11431:SF126">
        <property type="entry name" value="FERRITIN-2, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00210">
        <property type="entry name" value="Ferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00204">
        <property type="entry name" value="FERRITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50905">
        <property type="entry name" value="FERRITIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="9 13 14" id="KW-0408">Iron</keyword>
    <keyword evidence="3 14" id="KW-0409">Iron storage</keyword>
    <keyword evidence="6 13 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0934">Plastid</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Ferritin-like diiron" evidence="15">
        <location>
            <begin position="86"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00026060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR601519-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9698767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="258" mass="29210" checksum="3AE26662C60D59C7" modified="2024-01-24" version="1">MLLKSSASFSLSSSASFIGARKNAYSSTVLQFPWRNENVYGGKFVIKASRESGKASTLGAWFDPFEEVKKEHLIVPTCPQDSLARLKFSSPCETAINEQINVEYNVSYAYHTLYAYFDRDNIALKGLAKFCKESSTEEREHAEKLMEYQNKRGGKVKLRVIVRPPSEFDNHEKGDALHAMELALALEKLTTQKLLDLHKVAVENDDPHLVDFIESEFLTEQVESIKKVAEYVAQLRRLGKGYGVWHFDQMLLNHGVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DYS6</accession>
    <name>A0AA39DYS6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">tRNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_006975</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DYS6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02799">
        <property type="entry name" value="tRNA_bind_EMAP-II_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000225">
        <property type="entry name" value="tyrosine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002547">
        <property type="entry name" value="tRNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051270">
        <property type="entry name" value="Tyrosine-tRNA_ligase_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11586:SF47">
        <property type="entry name" value="NUCLEIC ACID-BINDING, OB-FOLD-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11586">
        <property type="entry name" value="TRNA-AMINOACYLATION COFACTOR ARC1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01588">
        <property type="entry name" value="tRNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50886">
        <property type="entry name" value="TRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1 3" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="TRNA-binding" evidence="5">
        <location>
            <begin position="95"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="58"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9701828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="263" mass="27997" checksum="AB57F0346E1A560C" modified="2024-01-24" version="1">MAAGNVLKGGGVLLVSCRNHASNVLSTSSSVPFRAGIKTEGSKRGMFWRHLSFCAVTTSSSTPPPPPPPPPPPPADASSPPPPLSSDDGDPIKDAASMLDIRVGLVLKAWRHEEADSLYVEEVDVGEPEPRIICSGLVKYVPLDHLQDKKVVVLANLKPRNMRGVKSCGMLMAASDASHENVELLVPPEGSIPGERIWFGSEDEKQSQPAAASPNQIQKKKIWELVQPHLKTDVSGVAVLGVNLMRTSAGVVVSRSLKNANIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E184</accession>
    <name>A0AA39E184_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Natural resistance associated macrophage protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_004598</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NRAMP (TC 2.A.55) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E184"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015086">
        <property type="term" value="F:cadmium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034755">
        <property type="term" value="P:iron ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001046">
        <property type="entry name" value="NRAMP_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11706:SF104">
        <property type="entry name" value="METAL TRANSPORTER NRAMP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11706">
        <property type="entry name" value="SOLUTE CARRIER PROTEIN FAMILY 11 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01566">
        <property type="entry name" value="Nramp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041256717" description="Natural resistance associated macrophage protein" evidence="7">
        <location>
            <begin position="28"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="104"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="142"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="180"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="207"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9702925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="267" mass="30300" checksum="A508EBCFC23C1264" modified="2024-01-24" version="1">MRRCREEYLTWARRLLWFMAELALIEADIQEVIGSAIEIKIFSRVLVPKFSSKTIRQAVEVIGCVIMPHNVFLHSALVQSRKINPHRKGRVQEALNYYSIESTVALLISFMINLFVTTIFAKGFCALVFNRFEASLDVLNEWLNVLQSMQIPFALIPLLTLVSKEQVMGVFKVGPLLERVAWTVAALVIVINGYLLLDFFMAEVDGVLFGFLVCAGTTLYVAFIFYLVSHGGALSSIWFSLVTSKLEVCFHWELRDLKNIIIILSTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E1F8</accession>
    <name>A0AA39E1F8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_006825</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E1F8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000021">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957:SF456">
        <property type="entry name" value="YPT_RAB-GAP DOMAIN OF GYP1P SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="2">
        <location>
            <begin position="365"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="150"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="549"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9701617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="693" mass="78659" checksum="7F5AF79E810D57CC" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E1L1</accession>
    <name>A0AA39E1L1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_004909</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E1L1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9703300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="81" mass="9456" checksum="5453FBC3BCF98D62" modified="2024-01-24" version="1">MIKELKTSGHVFTNKQQVEVVIKSLPKNYEHIVVNMTHDKSVKTFDDIVCHLELVVKRLMVARLNEQAHVIESNSRKNFRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E1M7</accession>
    <name>A0AA39E1M7_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_001680</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E1M7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06407">
        <property type="entry name" value="PB1_NLP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045012">
        <property type="entry name" value="NLP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055081">
        <property type="entry name" value="NLP1-9_GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000270">
        <property type="entry name" value="PB1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034891">
        <property type="entry name" value="PB1_NLP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003035">
        <property type="entry name" value="RWP-RK_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32002:SF35">
        <property type="entry name" value="PROTEIN NLP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32002">
        <property type="entry name" value="PROTEIN NLP8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22922">
        <property type="entry name" value="GAF_NLP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00564">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02042">
        <property type="entry name" value="RWP-RK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00666">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51519">
        <property type="entry name" value="RWP_RK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RWP-RK" evidence="6">
        <location>
            <begin position="582"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="domain" description="PB1" evidence="7">
        <location>
            <begin position="904"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="688"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="688"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="726"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="769"/>
            <end position="792"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9706271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="999" mass="110120" checksum="750E46C708CD6944" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YIW5</accession>
    <name>A0AA38YIW5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase</fullName>
            <ecNumber evidence="3">3.2.2.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_027334</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>NAD(+) + H2O = ADP-D-ribose + nicotinamide + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16301"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57967"/>
            <dbReference type="EC" id="3.2.2.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:16302"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the disease resistance TIR-NB-LRR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.6" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9671299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YIW5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061809">
        <property type="term" value="F:NAD+ nucleosidase activity, cyclic ADP-ribose generating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050832">
        <property type="term" value="P:defense response to fungus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043068">
        <property type="term" value="P:positive regulation of programmed cell death"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10140:FF:000007">
        <property type="entry name" value="Disease resistance protein (TIR-NBS-LRR class)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045344">
        <property type="entry name" value="C-JID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058546">
        <property type="entry name" value="RPS4B/Roq1-like_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058192">
        <property type="entry name" value="WHD_ROQ1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017:SF570">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN (TIR-NBS CLASS)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20160">
        <property type="entry name" value="C-JID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23286">
        <property type="entry name" value="LRR_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23282">
        <property type="entry name" value="WHD_ROQ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0611">Plant defense</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="TIR" evidence="14">
        <location>
            <begin position="27"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1048"/>
            <end position="1096"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1062"/>
            <end position="1096"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00061488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9671299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1096" mass="126555" checksum="8922B84315D10E88" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YJ44</accession>
    <name>A0AA38YJ44_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_025221</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YJ44"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9671413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="129" mass="14092" checksum="2A090E70D581D62D" modified="2024-01-24" version="1">MATPSSISLIFILVLVFSIPTFETRKVLSLKKAEVSSMDDEGHARVVNINGRLFTLHLPSINDHRMLTESVPSPGDGNSIHFPSINDHRMLPESIPSPGDGNSMHFPSTNDHRMLPESIPSPGDGNSLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YKU9</accession>
    <name>A0AA38YKU9_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_025794</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YKU9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044289">
        <property type="entry name" value="ATL67-70"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46592">
        <property type="entry name" value="RING-H2 FINGER PROTEIN ATL67"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46592:SF6">
        <property type="entry name" value="RING-H2 FINGER PROTEIN ATL67"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9672319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="153" mass="17705" checksum="F20A484240140A06" modified="2024-01-24" version="1">MFLFSSELIFHLHTHHLPILCNPYPSLLFFHHRSFPHLPFQPRPRLRHRHRLHHPSLHHPSCLLSLLSSSPPSPHHSQNPNFAANSSYSIVLPCVIFVAEDEDKDQNVVVGLDQAVINSYPKFPFSKSNTHLDSVCSICLYEYKDSEMLRMYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YL68</accession>
    <name>A0AA38YL68_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_025920</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YL68"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="361"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9672499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="451" mass="50867" checksum="F0AF7737C4B91C65" modified="2024-01-24" version="1">MEQTESDIKRVLQDYQKIGSGARCEEARIEVLNREIKVLEDRVRGSGSVEVERHRMRNLLDYQSTLIARAASRTELLAALHEDLLLLFIRRKQLRGSDGAEGSGVDAAPNVSLSSALSGGDDENQRFAKNCSELRSMHIMRMSHHEDLEEETTADQQPMPLGVKLLQINMNEDASLTSEDGCEYGDLQYKNNILDNIAMSILDPNANIDNIFKEAVENSNILCVESEGSKKSHRKASCAKRLFVDFDETGNPTGPSAGAFKRSINSYVCQLLPIRFKQIGEIPVEDYKSVVNALTERYSFDLDMSYTRKKISSCYRQHKYKLLIQVKKDLERGIEPQKPSYVDEEDWNAFVAKTKDEEFDRISKKNKASRSQQSALNRKDAQSTAHKRKKGDQGPEGISIWSKLHKPKQGMAKWAAWSGNLFIHVGISDYFLEADAVGVFNRVSREAVGHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YL73</accession>
    <name>A0AA38YL73_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_025902</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YL73"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009658">
        <property type="term" value="P:chloroplast organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010027">
        <property type="term" value="P:thylakoid membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040299">
        <property type="entry name" value="RF2K-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34938">
        <property type="entry name" value="PROTEIN FERTILITY RESTORER RF2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34938:SF1">
        <property type="entry name" value="PROTEIN FERTILITY RESTORER RF2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="138"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9672470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="170" mass="17586" checksum="CA7B1BF4B728E6C9" modified="2024-01-24" version="1">MSSLTASAIAISAKRPPEGECRGQIKILGPVPVGLYGRLLSFGGTQVIAPVGLYRQSLSFGRTQAIAHTRKQSASLICASALNARCGAEQTQTVTRQSSTITIAPVEGKEKSPELDDGGTGFPPRDDGDGGGGGGGGGGGWAGGFFFFGFLAFLGFLKDKESEGPYRDER</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YLX7</accession>
    <name>A0AA38YLX7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">glutathione transferase</fullName>
            <ecNumber evidence="1">2.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_026189</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the GST superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="1"/>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YLX7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03185">
        <property type="entry name" value="GST_C_Tau"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03058">
        <property type="entry name" value="GST_N_Tau"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000018">
        <property type="entry name" value="Glutathione S-transferase U20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000014">
        <property type="entry name" value="Tau class glutathione S-transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045074">
        <property type="entry name" value="GST_C_Tau"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045073">
        <property type="entry name" value="Omega/Tau-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11260">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE, GST, SUPERFAMILY, GST DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11260:SF764">
        <property type="entry name" value="GLUTATHIONE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01152">
        <property type="entry name" value="Main.3:_Omega-_and_Tau-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="5">
        <location>
            <begin position="3"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="6">
        <location>
            <begin position="88"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9672842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="219" mass="25397" checksum="23D495BDC824A439" modified="2024-01-24" version="1">MADEIILLDFWPSMFGMRVRVALAEKGLKYEYKEEDLRNKSPLLLEMNPVHKKIPVLIHNGKPICESLIIVQYIDEVWKDKAPLLPSDPYQRAQARFWADYVDKKLYELGRKIWSTKGEEQETAKKEFIECLKLLEGELGEKPYFGGEKIGFVDVALVTFSCWFHTYESFGNFSIEAECPKLIAWTKRCMEKESVSSSLEDPHKVHGFVMGIRKKLGIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YMQ2</accession>
    <name>A0AA38YMQ2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_023103</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">May be involved in plant defense signaling.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YMQ2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000228">
        <property type="entry name" value="Serine/threonine-protein kinase BIK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000032">
        <property type="entry name" value="Serine/threonine-protein kinase PBS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050823">
        <property type="entry name" value="Plant_Ser_Thr_Prot_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45621">
        <property type="entry name" value="OS01G0588500 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="82"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="370"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="29"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="386"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00054261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9673308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="418" mass="46084" checksum="E20871186DC443FD" modified="2024-01-24" version="1">MGNCCGAQANVNPASSKPPSPAELSKNNIKQDKSIKERSGSGSGNGSSNGGEKTETAETGKIITPNLKMFTFAELKSATRNFRPDTMLGEGGFGRVFKGWVDEKTYAPTKVSVGIPVAVKKSNPESEQGLKEWQSEVKFLGKFTHPNLVKLLGYCWEDKQFLLVYEYMQKGSLENHLFRVGAEPLAWEIRLKIAIGAARGLAFLHTSEKTVIYRDFKSSNILLDGDYNAKLSDFGLAKLGPSNGDSHVTTRIVGTYGYAAPEYIATGHLYVKSDVYGFGVVLLEMLTGKQALDMNRPPGQQILVEWAKPSLRHKKKLKKIMDLRLRDQYPLKAAAQAAELILNCLESDPKNRPPMEEVLETLKGINEIKEKPNSKEVKVATTQPQLHHHRSPYHPRHGGAGAGIGARTPPRGRMDRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YN50</accession>
    <name>A0AA38YN50_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NAD(P)H-quinone oxidoreductase subunit M, chloroplastic</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">NAD(P)H dehydrogenase subunit M</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">NADH-plastoquinone oxidoreductase subunit M</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_023220</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">NDH shuttles electrons from NAD(P)H:plastoquinone, via FMN and iron-sulfur (Fe-S) centers, to quinones in the photosynthetic chain and possibly in a chloroplast respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton translocation, and thus conserves the redox energy in a proton gradient.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a plastoquinone + NADH + (n+1) H(+)(in) = a plastoquinol + NAD(+) + n H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:42608"/>
            <dbReference type="Rhea" id="RHEA-COMP:9561"/>
            <dbReference type="Rhea" id="RHEA-COMP:9562"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17757"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:62192"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a plastoquinone + NADPH + (n+1) H(+)(in) = a plastoquinol + NADP(+) + n H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:42612"/>
            <dbReference type="Rhea" id="RHEA-COMP:9561"/>
            <dbReference type="Rhea" id="RHEA-COMP:9562"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17757"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:62192"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the chloroplast NDH complex, composed of a mixture of chloroplast and nucleus encoded subunits. Component of the NDH subcomplex A, at least composed of ndhH, ndhI, ndhJ, ndhK, ndhL, ndhM, ndhN and ndhO.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Chloroplast thylakoid membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Stromal side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NDH complex subunit M family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YN50"/>
    <dbReference type="SMR" id="A0AA38YN50"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016655">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018922">
        <property type="entry name" value="NdhM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36900">
        <property type="entry name" value="NAD(P)H-QUINONE OXIDOREDUCTASE SUBUNIT M, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36900:SF1">
        <property type="entry name" value="NAD(P)H-QUINONE OXIDOREDUCTASE SUBUNIT M, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10664">
        <property type="entry name" value="NdhM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-0521">NADP</keyword>
    <keyword evidence="8" id="KW-0618">Plastoquinone</keyword>
    <keyword evidence="6" id="KW-0874">Quinone</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="37"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="37"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9673521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="208" mass="24167" checksum="15D7927A3D657570" modified="2024-01-24" version="1">MAATSSYTACTKFSMLGWIGGKRELRKRRAFFISAQQQAEVEESQQVNAQEEEQEKMKQQGKQKLPRPVEPQVNVKSKNMSREYGGQWLSSVTRHVRIYAAYIDPVTCEFDQTQMDKLTLILDPTNEFVWTSETCNKVYAYFQELVDHYEGAPLTEYTLRLIGSDIEHYIRKLLYDGEIKYNMNARVLNFSMGKPRILFNDGLPQNVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YP00</accession>
    <name>A0AA38YP00_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Carbonic anhydrase</fullName>
            <ecNumber evidence="3 9">4.2.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Carbonate dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_023509</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">Reversible hydration of carbon dioxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>hydrogencarbonate + H(+) = CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:10748"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="EC" id="4.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the beta-class carbonic anhydrase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.1" evidence="3 9"/>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YP00"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015976">
        <property type="term" value="P:carbon utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00884">
        <property type="entry name" value="beta_CA_cladeB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1050.10:FF:000003">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1050.10">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045066">
        <property type="entry name" value="Beta_CA_cladeB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001765">
        <property type="entry name" value="Carbonic_anhydrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015892">
        <property type="entry name" value="Carbonic_anhydrase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036874">
        <property type="entry name" value="Carbonic_anhydrase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11002:SF19">
        <property type="entry name" value="BETA CARBONIC ANHYDRASE 6, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11002">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00484">
        <property type="entry name" value="Pro_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00947">
        <property type="entry name" value="Pro_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53056">
        <property type="entry name" value="beta-carbonic anhydrase, cab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00704">
        <property type="entry name" value="PROK_CO2_ANHYDRASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0702">S-nitrosylation</keyword>
    <keyword evidence="5 8 9" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601765-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9673996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="313" mass="34923" checksum="D6459845EDB0751E" modified="2024-01-24" version="1">MAALRPSSVSFDSAVSGPTNSFMGLLHKSPIFDSRKKLVRVGETHLGSLPSVKRNLVSRLEASSDSLGLGQHLTSNKMGNKMESLDKTDQGLDFFEELKHRFLCFKKQKYLEEPEHFQALANAQSPKFMVIACADSRVCPSNILGFQPGEAFMIRNVANLVPPVENGPSETNAALEFAVNTLEVENILVIGHSSCAGIEALVSMRDDVNSSSFVENWVANGKVAKLRTKAAAGHLSFYQQCKHCEKESINHSLLNLLTYPWIEDRERKGLLSIHGGYYDFLNCTFEKWTIDFKRSSIEKEGPKCLVKNRSFWC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YP82</accession>
    <name>A0AA38YP82_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_023585</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the amino acid/polyamine transporter 2 family. Amino acid/auxin permease (AAAP) (TC 2.A.18.2) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YP82"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000055">
        <property type="entry name" value="Amino acid permease 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48017">
        <property type="entry name" value="OS05G0424000 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0769">Symport</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="41"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="110"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="151"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="173"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="264"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="305"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="369"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="392"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="431"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="11">
        <location>
            <begin position="38"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9674121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="465" mass="51856" checksum="C19474AD0BF416DF" modified="2024-01-24" version="1">MAGNLNTNTLFVEQAPETIEKGNIGKNLDDDGRFKRTVLSLAWAISQLGWIAGPIVLVVFSFITYFTSTLLADCYRSPDPITGKRNYTYMDVVRANLGGRKVQLCGIAQYGNLIGVTIGYTITASISMVAVRRSNCYHKHGHQAKCNPSDYPYMIIFACIQIVLSQIPNFHKLSWLSILAAVMSFSYASIGIGLSIARVAGGAHARTTLTGRTVGVDLSGSEKVWRTFESIGNIAFAYAYSTVLVEIQDTLKSSPPENKVMKKATFVGISTTSLFYVLCGCVGYAAFGNDAPGNFLTGFGFFEPFWLIDLANVFIAIHLIGAYQVFCQPVFGFVEKWCNKRWPESKFITTERCIDVPLYGIYYLNLFRLVWRTVYVIVTAVIAMIFPFFNDVMGFLGAASFWPLTVYFPIEMHIARTKIPKFSFTWTWLKILSWTCLMVSVVAAAGSTQGLIKKIEKYKPFQTQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YPJ2</accession>
    <name>A0AA38YPJ2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">LOB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_023661</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LOB domain-containing protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YPJ2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004883">
        <property type="entry name" value="LOB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31304">
        <property type="entry name" value="LOB DOMAIN-CONTAINING PROTEIN 38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31304:SF1">
        <property type="entry name" value="LOB DOMAIN-CONTAINING PROTEIN 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03195">
        <property type="entry name" value="LOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50891">
        <property type="entry name" value="LOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LOB" evidence="3">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="176"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="185"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9674240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="222" mass="24160" checksum="879E5BB985240822" modified="2024-01-24" version="1">MSCNGCRVLRKGCSDSCILRTCLQWIENPEAQGYATLFVSKFFGRSDLMSFISAVPENKRPALFQSLLFEACGRTVNPVNGAVGLLWTGNWHMCQAAVDTVLAGGTLRPIPGILAGPIKPGFDEASATICTDAWKLRDAYSLTKPFMRDNASEEAKRIETQLPHLGISATPRFSNKINAGRGKRSGTSFCSEGSSGTMSYDGSDDDRNMSGCQERKLLNLFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YQN0</accession>
    <name>A0AA38YQN0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 14">Mitogen-activated protein kinase</fullName>
            <ecNumber evidence="2 14">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_023991</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="14">Activated by threonine and tyrosine phosphorylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Interacts with MKK3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="2 14"/>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YQN0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07858">
        <property type="entry name" value="STKc_TEY_MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000206">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000046">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="37"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00062946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9674792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="376" mass="43239" checksum="33F05998CD601A5D" modified="2024-01-24" version="1">MMFREMATPVEPPNGTRCEGKHYYSMWQTLFEIDTKYVPIKPIGRGAYGIVCSSVNKETNEKVAIKKIHNAFDNRVDALRTLREIKLLRHLRHENVISLKDVMMPIHRRSFKDVYLVYELMDTDLHQIIKSSQALTNDHCQYFLFQLLRGLKYLHSANILHRDLKPGNLLINANCDLKICDFGLARTSTGKGQFMTEYVVTRWYRAPELLLCCDNYGTSIDVWSVGCIFAELLGRKPIFPGTECLNQLKLIINILGSQREADIEFIDNPKARKYIKSLPFSPGTPFSRLYPNAHPMAIDLLQKMLIFDPSKRIGVTEALQHPFMSSLYDPSKNPPAQVPIDLDIDEDLGEEMIREMMWKEMLHYHPEAVTTIGEGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YRD3</accession>
    <name>A0AA38YRD3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">8-oxoguanine DNA glycosylase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_024196</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YRD3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034039">
        <property type="term" value="F:8-oxo-7,8-dihydroguanine DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006285">
        <property type="term" value="P:base-excision repair, AP site formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012904">
        <property type="entry name" value="OGG_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052054">
        <property type="entry name" value="Oxidative_DNA_repair_enzyme"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10242">
        <property type="entry name" value="8-OXOGUANINE DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10242:SF2">
        <property type="entry name" value="N-GLYCOSYLASE_DNA LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07934">
        <property type="entry name" value="OGG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55945">
        <property type="entry name" value="TATA-box binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="8-oxoguanine DNA glycosylase N-terminal" evidence="7">
        <location>
            <begin position="25"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9675147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="267" mass="30436" checksum="A8164121EB335AB4" modified="2024-01-24" version="1">MHSLKLPSTMKRRRPIPQPPSTLPTLSLVLTFPIGQIFRWKQTTPLQYTSVIGSHLISLKHLQNGDVAYLIHQSPSKENARSALLDFLNVGISLSEMWEVFKALNSRFAELTQHLGGARVLRQDPLECLFQFLCSLNNNIGRITKMVDFVLSLGNYLGSVGGFDFLQRKILEWLAYFREMDLQEVVDPLSILSGVGPKVVACIALFSLDQHHAIPVDKHVWQGFSNLIFKITFFKFSKNFKIPNLIFKMPSSKFKFPNLIFKIPFSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YSE9</accession>
    <name>A0AA38YSE9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_024541</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the DExH box helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YSE9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd17917">
        <property type="entry name" value="DEXHc_RHA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000060">
        <property type="entry name" value="DEA(D/H)-box RNA helicase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000526">
        <property type="entry name" value="DExH-box ATP-dependent RNA helicase DExH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000002">
        <property type="entry name" value="Putative ATP-dependent RNA helicase DHX36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059023">
        <property type="entry name" value="RNA_hel_CTD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF103">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26026">
        <property type="entry name" value="RNA_hel_CTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00358">
        <property type="entry name" value="DSRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="248"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="170"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1035"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="1062"/>
            <end position="1071"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00060772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9675672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1071" mass="121131" checksum="3C57436BB98020E7" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YSH5</accession>
    <name>A0AA38YSH5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">Laccase</fullName>
            <ecNumber evidence="4 13">1.10.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Benzenediol:oxygen oxidoreductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Diphenol oxidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Urishiol oxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_024579</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Lignin degradation and detoxification of lignin-derived products.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>4 hydroquinone + O2 = 4 benzosemiquinone + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:11276"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17594"/>
            <dbReference type="ChEBI" id="CHEBI:17977"/>
            <dbReference type="EC" id="1.10.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="13">Binds 4 Cu cations per monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Secreted</location>
            <location evidence="2 13">Extracellular space</location>
            <location evidence="2 13">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the multicopper oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.10.3.2" evidence="4 13"/>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YSH5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052716">
        <property type="term" value="F:hydroquinone:oxygen oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046274">
        <property type="term" value="P:lignin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13849">
        <property type="entry name" value="CuRO_1_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13875">
        <property type="entry name" value="CuRO_2_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13897">
        <property type="entry name" value="CuRO_3_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033138">
        <property type="entry name" value="Cu_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002355">
        <property type="entry name" value="Cu_oxidase_Cu_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034288">
        <property type="entry name" value="CuRO_1_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034285">
        <property type="entry name" value="CuRO_2_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034289">
        <property type="entry name" value="CuRO_3_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017761">
        <property type="entry name" value="Laccase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03389">
        <property type="entry name" value="laccase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF487">
        <property type="entry name" value="LACCASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00079">
        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00080">
        <property type="entry name" value="MULTICOPPER_OXIDASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0052">Apoplast</keyword>
    <keyword evidence="10 13" id="KW-0186">Copper</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 13" id="KW-0439">Lignin degradation</keyword>
    <keyword evidence="7 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 13" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Plastocyanin-like" evidence="16">
        <location>
            <begin position="17"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="14">
        <location>
            <begin position="143"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="15">
        <location>
            <begin position="410"/>
            <end position="543"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9675717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="560" mass="62609" checksum="12ED3F2CD78C8EFA" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YTL1</accession>
    <name>A0AA38YTL1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_025084</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9676384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YTL1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.870">
        <property type="entry name" value="Elongation Factor G (Translational Gtpase), domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9676384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="54" mass="5919" checksum="6510273657200CC6" modified="2024-01-24" version="1">MKLSPVVHAAVQCRVAFNLPKLVEGLKHLAKSDSVVVYIVEESGENITAKQSRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YUQ4</accession>
    <name>A0AA38YUQ4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_022109</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9676955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YUQ4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032872">
        <property type="entry name" value="WAK_assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025287">
        <property type="entry name" value="WAK_GUB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33138">
        <property type="entry name" value="OS01G0690200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33138:SF79">
        <property type="entry name" value="WALL-ASSOCIATED RECEPTOR KINASE GALACTURONAN-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13947">
        <property type="entry name" value="GUB_WAK_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14380">
        <property type="entry name" value="WAK_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041202849" description="non-specific serine/threonine protein kinase" evidence="7">
        <location>
            <begin position="30"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="Wall-associated receptor kinase galacturonan-binding" evidence="8">
        <location>
            <begin position="37"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="Wall-associated receptor kinase C-terminal" evidence="9">
        <location>
            <begin position="160"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF14380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9676955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="262" mass="29462" checksum="07E6F76DA9D43CEF" modified="2024-01-24" version="1">MHPNLSPSSFVIPAIIFLLSHHFPNFVCSDEFHYSNCNSPVECGGLGKIQYPFWVNGAQPDYCGHPRFKLDCLHGNFPTIQIMHRDYLVIDIDNDTQILKMMAIFHELSCSRFNITMDSTLFSYTPDVEIATVFYDCSSIDPHPGSYNFSCVINSFPDDAYLVTNTSTASEFASECNFNASFPVVVPKSADGVTEILREGFKVKWTVNKTECDECVGSGGRCGYNTTLYQPCCFCPDNPYRNKCPNLRSSVYPSSLSMLFRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YV17</accession>
    <name>A0AA38YV17_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">phenylalanine--tRNA ligase</fullName>
            <ecNumber evidence="5">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Phenylalanyl-tRNA synthetase beta subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_022246</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>tRNA(Phe) + L-phenylalanine + ATP = L-phenylalanyl-tRNA(Phe) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="5"/>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YV17"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009328">
        <property type="term" value="C:phenylalanine-tRNA ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.56.10:FF:000005">
        <property type="entry name" value="Phenylalanine--tRNA ligase beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.40.10:FF:000002">
        <property type="entry name" value="phenylalanine--tRNA ligase beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.56.10:FF:000004">
        <property type="entry name" value="Phenylalanyl-tRNA synthetase, beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.40.10">
        <property type="entry name" value="Phenylalanyl-trna Synthetase, Chain B, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005146">
        <property type="entry name" value="B3/B4_tRNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045060">
        <property type="entry name" value="Phe-tRNA-ligase_IIc_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004531">
        <property type="entry name" value="Phe-tRNA-synth_IIc_bsu_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020825">
        <property type="entry name" value="Phe-tRNA_synthase-like_B3/B4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041616">
        <property type="entry name" value="PheRS_beta_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040659">
        <property type="entry name" value="PhetRS_B1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005147">
        <property type="entry name" value="tRNA_synthase_B5-dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00471">
        <property type="entry name" value="pheT_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10947:SF0">
        <property type="entry name" value="PHENYLALANINE--TRNA LIGASE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10947">
        <property type="entry name" value="PHENYLALANYL-TRNA SYNTHETASE BETA CHAIN AND LEUCINE-RICH REPEAT-CONTAINING PROTEIN 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03483">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03484">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18262">
        <property type="entry name" value="PhetRS_B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17759">
        <property type="entry name" value="tRNA_synthFbeta"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00873">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00874">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56037">
        <property type="entry name" value="PheT/TilS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="Putative DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51483">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0436">Ligase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B5" evidence="16">
        <location>
            <begin position="303"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00033189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9677149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="556" mass="63179" checksum="96FA23884EE5834A" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YXH3</accession>
    <name>A0AA38YXH3_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_020651</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YXH3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000041">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000213">
        <property type="entry name" value="Tyrosine-sulfated glycopeptide receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046956">
        <property type="entry name" value="RLP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063">
        <property type="entry name" value="LRR RECEPTOR-LIKE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063:SF101">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE FLS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041413843" evidence="13">
        <location>
            <begin position="26"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="900"/>
            <end position="923"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9678528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1046" mass="116363" checksum="BF386C4C694516C0" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YXR6</accession>
    <name>A0AA38YXR6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">alanine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_020523</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.7" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YXR6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004813">
        <property type="term" value="F:alanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006419">
        <property type="term" value="P:alanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.980.10:FF:000004">
        <property type="entry name" value="Alanine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.980.10">
        <property type="entry name" value="Threonyl-trna Synthetase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018165">
        <property type="entry name" value="Ala-tRNA-synth_IIc_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050058">
        <property type="entry name" value="Ala-tRNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11777:SF9">
        <property type="entry name" value="ALANINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11777">
        <property type="entry name" value="ALANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="ThrRS/AlaRS common domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50860">
        <property type="entry name" value="AA_TRNA_LIGASE_II_ALA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Alanyl-transfer RNA synthetases family profile" evidence="11">
        <location>
            <begin position="51"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="262"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9678374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="503" mass="56305" checksum="52FD68CA47F90177" modified="2024-01-24" version="1">MGISFSRQKPLDLTKFLVLPPSLQLNFCINPFKYENIFCKQKRVWKISSYDRRKLIAPNHTCTHMLNFALREVLGNHVDQKGSTVLPEKLRFDFSHGKPIHPDHLRRIESIVNDQIEDELDVYGKEAALADAKRINGVRAVFGEVYPDPVRVLTIGQKVEDLLADPDNEEWLSVSAELCGGTHISNTREAKAFALLSEEGITKGIRRITTVTTDRAFKAMELAFSLELEVDAASEAEGSLLEKKVSSLRSRVDAAPIPAPKKADLRTKISLLQDQVRKEQKKIAEENIQKAVKVATEMADGAASDGKAFCISLVDVGLDTTAVREAVLKVIEQKGISVTVFSTDETTNKAVVYAGVPENGEKFKQLELSEWLTAALGLYNIRIPTNYLWLCINETELIDGTAVFRYNAMQSCLMKQRERLAMTLVDKEMEIYIHSLTRVDYLSNQRMSVVFCTLDLMPSISLSPFFKEPQFKLCCPTPSQCLPSLDPGIVVPSSRLPKGLKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YYT6</accession>
    <name>A0AA38YYT6_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_021018</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YYT6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9678973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="106" mass="11514" checksum="2AC28ACBBE484A2B" modified="2024-01-24" version="1">METMALCEGKNKENIPPFSSKQTHPVLGITPSSTKTIRRKFRKPLADITNPFNPSPQSASASASHDGSQLPPSVAVCAPSSRKRKAAGDTDSMQSTLFKALRRDFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YZW1</accession>
    <name>A0AA38YZW1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">HMA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_021456</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the HIPP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YZW1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051863">
        <property type="entry name" value="HIPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45811">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45811:SF80">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0488">Methylation</keyword>
    <keyword evidence="4" id="KW-0636">Prenylation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMA" evidence="7">
        <location>
            <begin position="3"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="69"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9679543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="127" mass="14399" checksum="411B241CDAEB9506" modified="2024-01-24" version="1">METKKIILKLEVFDDKAKQKAMKNVSSLPGVTSISMDMKDKKLTVIGDVDPVCVVGKLRKSCHTEILSVGPAKEPEKKEPKKPKKEEKDPKDQWADFVKARQAYSPYYAPHYYIRSVEEDPNACVIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z2P6</accession>
    <name>A0AA38Z2P6_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_020085</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z2P6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000357">
        <property type="entry name" value="Caffeic acid 3-O-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000061">
        <property type="entry name" value="Caffeic acid O-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016461">
        <property type="entry name" value="COMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001077">
        <property type="entry name" value="COMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012967">
        <property type="entry name" value="COMT_dimerisation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11746">
        <property type="entry name" value="O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08100">
        <property type="entry name" value="Dimerisation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00891">
        <property type="entry name" value="Methyltransf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005739">
        <property type="entry name" value="O-mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51683">
        <property type="entry name" value="SAM_OMT_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="O-methyltransferase dimerisation" evidence="6">
        <location>
            <begin position="30"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="O-methyltransferase C-terminal" evidence="5">
        <location>
            <begin position="173"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR005739-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9680998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="394" mass="43341" checksum="49AD94B76D53BC54" modified="2024-01-24" version="1">MGSSTEERQCSHGSTGEEEVQEAEHYCNAMQLVASSLLPMVMQTAIELGLFHIIAKAGQASASEIASQLPANNPAAPVMLDRILYFLTSHSVLTCSALNADGGRLKRVYGLTPVSKYFVPDQDGISLSPLLTLIQDKVFMDSWSVCSIFFVLSTLSVGSQLTPVISQYFFRCHLKNSIIEGGIPFNRAHGVNAFEYPGKNPRFNQAFNTAMLNHTIMITNKIVESYKGFGNLKQVVDVGGGLGTTLGILTSKYPSIKAINFDLPHVIQDALPYPGVEHLGGDMFESVPNGEAIFMKWILHDWSDEHCLKLLKNCYKALPEQGMAIVVEGFLPEIPEGSASVQALCEGDLIMMTQSPGGRERTRQEFLDLAMAAGFAGIRFECLVYNYWIMEFFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z307</accession>
    <name>A0AA38Z307_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_020350</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9681435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z307"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46567">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46567:SF1">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9681435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="68" mass="7249" checksum="4D1A4BD040C052AF" modified="2024-01-24" version="1">MPLQQDHDIEIDPWTLLEDGAGAGPSSGNTAVIGSGDHANLRASSWLRGTVRVRRTDLTYIGAVDDDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z4R5</accession>
    <name>A0AA38Z4R5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">S-adenosylmethionine-dependent methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_018231</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z4R5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37886">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT METHYLTRANSFERASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37886:SF1">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT METHYLTRANSFERASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9682250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="331" mass="36812" checksum="5CDB2DFC2C2D3F2F" modified="2024-01-24" version="1">MALSSKGGVSTVTVLVLSISVAAVFLFFLLSSLSPCSCPIPPAPSSVGSDVGDGGGGGGRSGSGAFEGRISTSAEDVEWVKDQITVNGLRMQDNVLRKGINPRTRQQQLQDLIEFKGISHYEEPEVGNHTALPCPGELLVEQHHSNYGEPWAGGRDVFEFLAESVHLSPDSRVLEIGCGTLRVGLHFIRYLNPEHYHCLERDELSLMAAFRYELPSQGLLYKRPLIVRGEDMDFSRFGSGVVYDLMYASAVFLHMPDKLVWVGLERLANKLKPYEGRIFVSHNIKFCSRLGGDECTKRLTSLGLEYVAKRTHDSLLFNHYEIWFEFRRSKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z9W1</accession>
    <name>A0AA38Z9W1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_017224</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z9W1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000242">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF415">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="44"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="75"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="110"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9685111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="195" mass="21800" checksum="1D8172F030A9E480" modified="2024-01-24" version="1">MQMVGIEPDEISLVSVLPACAQLGALELGKWIHFYADKAGFLRNICVCNALIEMYAKRGSIDEGRRLFDQMNERDVISWSTMIVGLANHGRAREAIELFQEMQNAKIEPNIITFVSLLSACAHAGLLNEGLRYFESMKRDYNIEPGVEHYGCLVNLLGLSGRLDQALELIKTMPMKLTSPKIAVKPDTHQANFCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZD57</accession>
    <name>A0AA38ZD57_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_015213</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="5">Forms heterodimers with BZIP18, BZIP43 and VIP1/BZIP51.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZD57"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14703">
        <property type="entry name" value="bZIP_plant_RF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000057">
        <property type="entry name" value="Basic leucine zipper 61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044759">
        <property type="entry name" value="bZIP_RF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052483">
        <property type="entry name" value="bZIP_transcription_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46391">
        <property type="entry name" value="BASIC LEUCINE ZIPPER 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46391:SF20">
        <property type="entry name" value="BASIC LEUCINE ZIPPER 61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="7">
        <location>
            <begin position="170"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="104"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="269"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="105"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="145"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="283"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00066000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9686204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="295" mass="33172" checksum="480A9605E7B89091" modified="2024-01-24" version="1">MAQLPPKVPAMAANWPSFPHQKIPSMGNLAPPATQNPSWVDEFLDFSSAKRGTHRRSASDSIAFLEAPDDCRNSGAAPRPAGAGNEFDQFDDEQFMSMFTDAMAPTISSSNPSTPSDHYSFNEEKTTTIEQQQQQQQLKNESEEVQSSCKLESQTPPQPSMTTSNDRIVDPKRVKRILANRQSAQRSRVRKLQYISELERSVTSLQTEVSVLSPRVAFLDHQRLLLNVDNSALKQRIAALAQDKIFKDAHQDALKREIERLRQVYHQQNLKTTENAAPPTASDVKDFKEEDVLNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZDL6</accession>
    <name>A0AA38ZDL6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Vacuolar cation/proton exchanger</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_015804</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Vacuolar cation/proton exchanger (CAX). Translocates Ca(2+) and other metal ions into vacuoles using the proton gradient formed by H(+)-ATPase and H(+)-pyrophosphatase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family. Cation/proton exchanger (CAX) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZDL6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009705">
        <property type="term" value="C:plant-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015369">
        <property type="term" value="F:calcium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.30:FF:000008">
        <property type="entry name" value="Vacuolar cation/proton exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.30:FF:000012">
        <property type="entry name" value="Vacuolar cation/proton exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="entry name" value="NCX, central ion-binding region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004713">
        <property type="entry name" value="CaH_exchang"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004798">
        <property type="entry name" value="CAX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00846">
        <property type="entry name" value="caca2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00378">
        <property type="entry name" value="cax"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31503">
        <property type="entry name" value="VACUOLAR CALCIUM ION TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31503:SF48">
        <property type="entry name" value="VACUOLAR CATION_PROTON EXCHANGER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12" id="KW-0050">Antiport</keyword>
    <keyword evidence="8 12" id="KW-0106">Calcium</keyword>
    <keyword evidence="6 12" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="10 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <keyword evidence="5 12" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="105"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="135"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="169"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="204"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="239"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="297"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="327"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="364"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="393"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="14">
        <location>
            <begin position="105"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="14">
        <location>
            <begin position="296"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="36"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU365028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9687093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="447" mass="48439" checksum="97960F47CB5B1435" modified="2024-01-24" version="1">MDNQNSQMVGIHSHLEMGSLEERAVLEFEDESLFSPDSASRKTHSREGPLVLAPSPASEPSGTKCTSNSIFNSIKIVVFSTKINLLVPFGPAAILVDKFTDNHSWVFLLSLLGIIPLAERLGYATEQLALFTGPTVGGLLNATFGNATELIISVYALKNGMIRVVQQSLLGSILSNMLLVLGCAFFSGGMVFSKKEQVFSKAAAGVNSGLLLMAVMGLLFPAVLHFTHTELHFGSSELALSRFSSCIMLVAYAAYLVFQLKSQKNLYVPVDEGGNQTEGDGDGDEDEAPEISKWESIIWLTILTLWISVLSEYLVNAIEGASVAWKMPIAFISVILLPIVGNAAEHASAIIFAMKDKLDISLGVAIGSSTQISMFGIPFCVVVGWIMGRPMDLNFQLFETATLFMTVIVVAFMLQVCPRRKLSLSNSISSIILKENLFNVNARILVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZE07</accession>
    <name>A0AA38ZE07_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_015963</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZE07"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003832">
        <property type="entry name" value="DUF212"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02681">
        <property type="entry name" value="DUF212"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9687286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="147" mass="16040" checksum="AC89F0954CEC377C" modified="2024-01-24" version="1">MGFFVFSSTFRAFNFKSSPSFFRPRSSVVSLSISNPQNPNPKPSSYSLVLNPFVVFHTIRGLASRGFEFKEEFNGGYYQNGALGGMPSSHTTLCMTLMTFMVLCHGVADSFFPVCVRCAGMQVEVLVGAVLGIMIVCIYCQGCLVAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZEB7</accession>
    <name>A0AA38ZEB7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_016083</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZEB7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF78">
        <property type="entry name" value="OS12G0587133 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="361"/>
            <end position="641"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9687450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1093" mass="125711" checksum="23A378E4A93E964E" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZFI1</accession>
    <name>A0AA38ZFI1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_014034</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZFI1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035251">
        <property type="term" value="F:UDP-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050481">
        <property type="entry name" value="UDP-glycosyltransf_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48049">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48049:SF60">
        <property type="entry name" value="UDP-GLYCOSYLTRANSFERASE 91B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9688081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="170" mass="18792" checksum="AE929E86244C9C41" modified="2024-01-24" version="1">MPAPRGDRIQDCMDSEASDFFRLAKVIEGCRFGDSLSLLQKLYQQPVVPIGLLPTEVNDSEGDESWGTLRQWLYEKTENSVLYVALGTELTLKKSGLPFVWVVKTKDDPFITGFEARQILAHPSVGGFLTHCGWSSVIEALGLGRVLVLFPGAFQISGRWQDSLKVSKSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZG36</accession>
    <name>A0AA38ZG36_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Subtilisin-like protease SBT6.1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PVL29_014216</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the peptidase S8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZG36"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07479">
        <property type="entry name" value="Peptidases_S8_SKI-1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000011">
        <property type="entry name" value="subtilisin-like protease SBT6.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055143">
        <property type="entry name" value="MBTP1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057060">
        <property type="entry name" value="MBTPS1_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057032">
        <property type="entry name" value="MBTPS1_4th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050131">
        <property type="entry name" value="Peptidase_S8_subtilisin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034185">
        <property type="entry name" value="Site-1_peptidase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806:SF7">
        <property type="entry name" value="MEMBRANE-BOUND TRANSCRIPTION FACTOR SITE-1 PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806">
        <property type="entry name" value="PEPTIDASE S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23001">
        <property type="entry name" value="MBTP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23094">
        <property type="entry name" value="MBTPS1_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23090">
        <property type="entry name" value="MBTPS1_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 12" id="KW-0645">Protease</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041458033" description="Subtilisin-like protease SBT6.1" evidence="14">
        <location>
            <begin position="28"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1007"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="domain" description="Membrane-bound transcription factor site-1 protease-like N-terminal" evidence="16">
        <location>
            <begin position="58"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="15">
        <location>
            <begin position="210"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="MBTPS1 third" evidence="18">
        <location>
            <begin position="490"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="domain" description="MBTPS1 fourth" evidence="17">
        <location>
            <begin position="619"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="12">
        <location>
            <position position="219"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="12">
        <location>
            <position position="250"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="12">
        <location>
            <position position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF23001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF23090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF23094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ9688431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="21">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1046" mass="117029" checksum="139B3E5DEC276D2B" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZIE9</accession>
    <name>A0AA38ZIE9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Plasma membrane ATPase</fullName>
            <ecNumber evidence="16">7.1.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PVL29_014880</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15 16">
            <text>ATP + H2O + H(+)(in) = ADP + phosphate + 2 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:20852"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Cell membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIIA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.1" evidence="16"/>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9689404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZIE9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008553">
        <property type="term" value="F:P-type proton-exporting transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120029">
        <property type="term" value="P:proton export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02076">
        <property type="entry name" value="P-type_ATPase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000045">
        <property type="entry name" value="ATPase 4 plasma membrane-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000004">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000004">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000211">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006534">
        <property type="entry name" value="P-type_ATPase_IIIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01647">
        <property type="entry name" value="ATPase-IIIA_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42861">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 16" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="13 16" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 16" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 16" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 16" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="98"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="133"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="276"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="314"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="682"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="747"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="788"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="821"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="854"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="17">
        <location>
            <begin position="50"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU362083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ9689404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="986" mass="108647" checksum="8DB5BBC939378BD7" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZIQ5</accession>
    <name>A0AA38ZIQ5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PGG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_012474</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9689821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZIQ5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026961">
        <property type="entry name" value="PGG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24177">
        <property type="entry name" value="CASKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24177:SF314">
        <property type="entry name" value="PROTEIN ACCELERATED CELL DEATH 6-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13962">
        <property type="entry name" value="PGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="455"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="488"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="532"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="568"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="domain" description="PGG" evidence="3">
        <location>
            <begin position="445"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="298"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="298"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9689821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="657" mass="75063" checksum="B01BF3A3B570150E" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZL80</accession>
    <name>A0AA38ZL80_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_013004</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZL80"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041430705" evidence="2">
        <location>
            <begin position="23"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9690627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="80" mass="8915" checksum="C059B36511A5F761" modified="2024-01-24" version="1">MAILKLFVCSILVLLLSARTQPRPLNPHVESRNLSTSFQASIKASREPFEVKMNEENPMKTENSKPVRLSPGGPDPVHHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZLL3</accession>
    <name>A0AA38ZLL3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_013485</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZLL3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16454">
        <property type="entry name" value="RING-H2_PA-TM-RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15710">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE PRAJA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15710:SF196">
        <property type="entry name" value="F6A14.12 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="182"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9691320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="230" mass="26605" checksum="EE9F3DF49906D2C8" modified="2024-01-24" version="1">MPNNRHQEHLVMDDIQRVESWDFIPIRLLRYETAQYYFPSTPGDTVGMLIKEDDTFIMEQIFKIRHDVLISYPAADSFFSPVLRNYGVSPDDQVRIIRQVSALACSMKLPIVRSIAVFIMDVIVVDINESQELWEILDLYGEEDHEDIDMDMEEDQMIKTVPATKSSIDKLEKVGLDLSQDCSICLESLPIGLQVVRMPCSHVFHSDCIVKWLERSCLCPMCRFQMPCVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZNI9</accession>
    <name>A0AA38ZNI9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">polynucleotide adenylyltransferase</fullName>
            <ecNumber evidence="5">2.7.7.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PVL29_011349</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>RNA(n) + ATP = RNA(n)-3'-adenine ribonucleotide + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11332"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17347"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="ChEBI" id="CHEBI:173115"/>
            <dbReference type="EC" id="2.7.7.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the poly(A) polymerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.19" evidence="5"/>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZNI9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990817">
        <property type="term" value="F:poly(A) RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031123">
        <property type="term" value="P:RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05402">
        <property type="entry name" value="NT_PAP_TUTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.590:FF:000002">
        <property type="entry name" value="Nuclear poly(A) polymerase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.460.10:FF:000002">
        <property type="entry name" value="Poly(A) polymerase alpha, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1410.10:FF:000001">
        <property type="entry name" value="Putative poly(A) polymerase gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.590">
        <property type="entry name" value="Poly(A) polymerase predicted RNA binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011068">
        <property type="entry name" value="NuclTrfase_I-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007012">
        <property type="entry name" value="PolA_pol_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048840">
        <property type="entry name" value="PolA_pol_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007010">
        <property type="entry name" value="PolA_pol_RNA-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10682">
        <property type="entry name" value="POLY A POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10682:SF22">
        <property type="entry name" value="POLYNUCLEOTIDE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04928">
        <property type="entry name" value="PAP_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20750">
        <property type="entry name" value="PAP_NTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04926">
        <property type="entry name" value="PAP_RNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55003">
        <property type="entry name" value="PAP/Archaeal CCA-adding enzyme, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81631">
        <property type="entry name" value="PAP/OAS1 substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Poly(A) polymerase nucleotidyltransferase" evidence="18">
        <location>
            <begin position="20"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="Poly(A) polymerase central" evidence="17">
        <location>
            <begin position="219"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="Poly(A) polymerase RNA-binding" evidence="16">
        <location>
            <begin position="367"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="493"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="622"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="563"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="622"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="643"/>
            <end position="675"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF04926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF20750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ9692237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="756" mass="84542" checksum="ADC121C9C2B2E560" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZNU7</accession>
    <name>A0AA38ZNU7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GRAM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_013756</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GEM family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZNU7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037848">
        <property type="entry name" value="GEM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004182">
        <property type="entry name" value="GRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31969">
        <property type="entry name" value="GEM-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02893">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00568">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GRAM" evidence="2">
        <location>
            <begin position="88"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9691664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="210" mass="23618" checksum="1EDAAB632E1D9E7C" modified="2024-01-24" version="1">MKNQLAGHVFRTPISCQCDKPSSSSQSLVVTHDKVDRMKNMMNKHGQKADNFVHGIREHLRIGSTLSETVKGKLSMGARILQLGGVKRVFKQIFGIGEGEKLLKASQCYLSTTAGPIAGLLFISTHRVAFCSERSIKISSPNGELVRIHYKVSIPLRNIKRVNQSANVKMPSQKYMEIVTIDDFEFWYMGFLNYQKAFHCLQQALSQSQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZP18</accession>
    <name>A0AA38ZP18_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Jacalin-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_011290</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the jacalin lectin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZP18"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09612">
        <property type="entry name" value="Jacalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.100.10.30:FF:000001">
        <property type="entry name" value="Jacalin-related lectin 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.100.10.30">
        <property type="entry name" value="Jacalin-like lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001229">
        <property type="entry name" value="Jacalin-like_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033734">
        <property type="entry name" value="Jacalin-like_lectin_dom_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036404">
        <property type="entry name" value="Jacalin-like_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47293:SF15">
        <property type="entry name" value="JACALIN-RELATED LECTIN 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47293">
        <property type="entry name" value="JACALIN-RELATED LECTIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01419">
        <property type="entry name" value="Jacalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00915">
        <property type="entry name" value="Jacalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51101">
        <property type="entry name" value="Mannose-binding lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51752">
        <property type="entry name" value="JACALIN_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0430">Lectin</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Jacalin-type lectin" evidence="4">
        <location>
            <begin position="1"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="165"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="172"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9692142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="184" mass="20182" checksum="E9891F703B702CA0" modified="2024-01-24" version="1">MVVGTWGGDGGSSWDDGSYSGVREITIVYDRCIDWIRVVYDKNGKPVIGQKHGGVGGNKTTEIKLQYPEEILISVSGHYCPVVYGGSPVIRSLTFKSNRRTFGPFGVEEGTPFSLSMDGGQIVGFQGRSGWYLDAIGFHLSRARSTKLFEKVQKKLQKIASMAISAPKDSEATPQVKKNQSMRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZQQ7</accession>
    <name>A0AA38ZQQ7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Exocyst subunit Exo70 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_012326</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Component of the exocyst complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the EXO70 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9693496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZQQ7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.170">
        <property type="entry name" value="Exocyst complex component Exo70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004140">
        <property type="entry name" value="Exo70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046364">
        <property type="entry name" value="Exo70_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12542">
        <property type="entry name" value="EXOCYST COMPLEX PROTEIN EXO70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12542:SF85">
        <property type="entry name" value="EXOCYST SUBUNIT EXO70 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03081">
        <property type="entry name" value="Exo70_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20669">
        <property type="entry name" value="Exo70_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex subunit Exo70 C-terminal" evidence="4">
        <location>
            <begin position="277"/>
            <end position="632"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU365026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9693496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="668" mass="75901" checksum="C6716A253804608F" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZTI9</accession>
    <name>A0AA38ZTI9_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_010155</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZTI9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35286">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35286:SF1">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9694527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="192" mass="21545" checksum="AF79E59E77A77F23" modified="2024-01-24" version="1">MSSYNNGTANFDNLLMQTLMGRLQIRPQNPLLPQSLEDLLFDPAITSHLSSDDDNDDDSKTQLAKEESKLEKEIIRIILSGNIQSLKPNSGQAVTIGEHHICVGFHEETGSDYRVWEWHGHIMLFDEENGYTPEYIYGNYFERVPVKAAAKVSGEEKDEKVGNMGLRELIESGDSGSSRIIHRNMNSGSPSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZUG1</accession>
    <name>A0AA38ZUG1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_010761</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZUG1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004158">
        <property type="entry name" value="DUF247_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31549:SF191">
        <property type="entry name" value="DUF247 DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31549">
        <property type="entry name" value="PROTEIN, PUTATIVE (DUF247)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03140">
        <property type="entry name" value="DUF247"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="424"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="241"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="251"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9695446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="450" mass="51012" checksum="DF3363AA5E4CD46C" modified="2024-01-24" version="1">MDGVGATKGEISYDEHLIDIIDPQVWNDTHKASDARERTPSIPKIPTALKNCQNQKLQGWYKPNVISIGPYYYGGDHLQEAQKLKSGHAMKFIEESKQNRDALFLKIIKKTPELKKCYCYHNKTISDSSDEKLATIFLLDGCFLLHFINYSSEKDGSKLLGLTNHEMTHIKQDLFLLENQLPYKVLKLLLKGAKNSNPMEEKIKDFVASHAPFHGGIRVKKVNPKPYHLLHYLQGIILDKPETSSPTQKQNEGDKGHRRGRLEGDSCKNIQELKKVGIHFKPSPTSYLTDISFEPHFGITGCLKLPTFSMNTSTMIIFLNLIAFESSDTTSNLGVISYLCFLDSLIDRWDDVKELQAAGIIRNFVGERADVAQFFNSVCSKLVPNPSAYDDVKEQIQNHVDRHQNCTLRTWYIQSKQKYFSSPWSFIALTAAVIGLLLTGIQAYTSLFFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZVU5</accession>
    <name>A0AA38ZVU5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">CNNM transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_008468</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZVU5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030026">
        <property type="term" value="P:intracellular manganese ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010960">
        <property type="term" value="P:magnesium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04590">
        <property type="entry name" value="CBS_pair_CorC_HlyC_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.580.10:FF:000021">
        <property type="entry name" value="DUF21 domain-containing protein At4g14240-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.580.10:FF:000042">
        <property type="entry name" value="DUF21 domain-containing protein isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045095">
        <property type="entry name" value="ACDP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002550">
        <property type="entry name" value="CNNM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044751">
        <property type="entry name" value="Ion_transp-like_CBS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12064">
        <property type="entry name" value="METAL TRANSPORTER CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12064:SF97">
        <property type="entry name" value="METAL TRANSPORTER CNNM-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01595">
        <property type="entry name" value="CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51846">
        <property type="entry name" value="CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0129">CBS domain</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 8 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="32"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="117"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="147"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="CNNM transmembrane" evidence="11">
        <location>
            <begin position="28"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="330"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="377"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="456"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="347"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="380"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9696240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="504" mass="54540" checksum="993F1B5D41E29559" modified="2024-01-24" version="1">MSLLNALTLGSMPTTGEFVLRTEDIEFGSVWWFVYAGVSCVLVLFAGIMSGLTLGLMSLGLVELEILQRSGTSAEKKQAAAILPVVQKQHQLLVTLLLCNACAMEALPIYLDKIFHPFVAILLSVTFVLAFGEIIPQAICTRYGLSVGANFVWLVRILMIICYPIAFPIGKVLDAVLGHNDALFRRAQLKALVSIHGQEAGKGGELTHDETTIISGALDLTEKTAEEAMTPIESTFSLDVNSKLDWEAIGKILARGHSRVPVYSGNPKNIIGLLLVKSLLTVRAETETPVSAVSIRKIPRVPADMPLYDILNEFQKGSSHMAAVVKVKGKNKNPLPKGDGERFEENSVANGNSQYTTPLLAKDNDRSENVVVDIDKVPKPTNTNKQIPSQQNGATTNSLPHLPEDIEDGEVIGIITLEDVFEELLQEEIVDETDVYVDVHKRIRVAAAAAASFVARAPSSRRLTGQKPAGGQGRQGTPKKSAEDDSFWAKSPGNLGEALLGSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZW55</accession>
    <name>A0AA38ZW55_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Membrane-anchored ubiquitin-fold protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_008556</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">May serve as docking site to facilitate the association of other proteins to the plasma membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZW55"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd01814">
        <property type="entry name" value="Ubl_MUBs_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017000">
        <property type="entry name" value="MUB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040015">
        <property type="entry name" value="UBL3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039540">
        <property type="entry name" value="UBL3-like_ubiquitin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13169:SF12">
        <property type="entry name" value="MEMBRANE-ANCHORED UBIQUITIN-FOLD PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13169">
        <property type="entry name" value="UBIQUITIN-LIKE PROTEIN 3 HCG-1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13881">
        <property type="entry name" value="Rad60-SLD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF032572">
        <property type="entry name" value="MUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="6" id="KW-0564">Palmitate</keyword>
    <keyword evidence="8" id="KW-0636">Prenylation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="10">
        <location>
            <begin position="8"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR032572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9696390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="119" mass="13217" checksum="FE1C62C83DCE0160" modified="2024-01-24" version="1">MAEGEEHIELKFRIYDGTDIGHRTYASSTTVATLKQRLVAEWPPDKTVIPKSVNDMKLIHAGKVLENSKTLAESRITFGDLPSGVITMHVVVQPPVAKKKTDKNQDEMPKQNSCSCIIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZXJ0</accession>
    <name>A0AA38ZXJ0_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_009082</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the MS5 protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZXJ0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044961">
        <property type="entry name" value="MS5/SDI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36326">
        <property type="entry name" value="PROTEIN POLLENLESS 3-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36326:SF14">
        <property type="entry name" value="PROTEIN SULFUR DEFICIENCY-INDUCED 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13374">
        <property type="entry name" value="TPR_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 7" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="153"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9697155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="364" mass="41771" checksum="C92B299CFE92D702" modified="2024-01-24" version="1">MQESSRRGDKDPFCAIHKLPSGDTPYARAKRALMVEKDPEAAIVLFWSAINARDRVDSALKDMVVVMKRLDRSEEAIEAIKSFRNLCPKQAQESLDNILIDLYKKCGRTNEHIDMLKHKLRLIYQGKAFNGKPTKTARSNRKKFQITIKQETSRILGNLGWVYMQKSNYMAAEVVYKKAQMIDPDANKACNLALCLINQGRYTEAHSILKEVLQGKLPNSEDCKAQNRAQELMLEVEPKWLPPSETIKLEGFDLENDFSDGFEKVVYKKAQMIDPDANKACNLALCLINQGRYTEAHSILKEELMLEVEPKWLPLSETINLEAFELEDDFSDGFEKVLNIWAPFRTKRLPIFEEISSYRNQLAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZXR7</accession>
    <name>A0AA38ZXR7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Retrotransposon Copia-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_008861</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZXR7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34222:SF40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34222">
        <property type="entry name" value="GAG_PRE-INTEGRS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="222"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="273"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="284"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9696860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="383" mass="42753" checksum="2249751B0E6FADCD" modified="2024-01-24" version="1">MAAQRGNNHESSQSATHERVSEIHSSMGPVGAFDNSPLHLTVENLNGKNNREWAQAIKLVIDGKGKLGLLTGETRRPPPTDVAASQKWQSENSFITSCLINSMKLAIGKTYMFLPTAKDMKQGDREVTEYYTEMLGLWQDLDLSCEEEWECTGDSVCFKKKMENERVFEFLAGLNREFDDVRSKVLSHRPLSSIREVFSEVRREESRRRVMLDLSFGPEGSALLTHRPHTAAGRGPHAAGSSGPSPRQSKRTYCEHCKKLGHTKDTCWALHGKPANWKPRQPNKAHSNQASTETRADKTPTEICQSTSSVRFNSDQLAKLYELFFNFQTSGQSSTTLSSGSLAKKGTFLTVLSTMSQITPWIIDSGASDHMTNAHHLFSTHSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A0L9</accession>
    <name>A0AA39A0L9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PVL29_007575</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A0L9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009742">
        <property type="term" value="P:brassinosteroid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000016">
        <property type="entry name" value="probable serine/threonine-protein kinase At4g35230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000154">
        <property type="entry name" value="probable serine/threonine-protein kinase At4g35230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000069">
        <property type="entry name" value="probable serine/threonine-protein kinase At5g41260"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045845">
        <property type="entry name" value="BSK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025749">
        <property type="entry name" value="Sphingomyelin_synth-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058209">
        <property type="entry name" value="TPR_BSK1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45863">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE BSK5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45863:SF7">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE BSK5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14360">
        <property type="entry name" value="PAP2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25575">
        <property type="entry name" value="TPR_BSK1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1070">Brassinosteroid signaling pathway</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0519">Myristate</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="109"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="169"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="225"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="376"/>
            <end position="631"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ9698569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="808" mass="91096" checksum="47F902347009212A" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A0M4</accession>
    <name>A0AA39A0M4_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_007721</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A0M4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9698790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="57" mass="6511" checksum="36397047D970E840" modified="2024-01-24" version="1">MIRELKTSGHVFTDEQQVEAAVKSLPKSCEHMVVNMTHNKSVKTFDNIVCHLELEVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A2T7</accession>
    <name>A0AA39A2T7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transcription factor UPBEAT1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_005611</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9699827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A2T7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11444">
        <property type="entry name" value="bHLH_AtIBH1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044549">
        <property type="entry name" value="bHLH_AtIBH1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044660">
        <property type="entry name" value="IBH1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33124">
        <property type="entry name" value="TRANSCRIPTION FACTOR IBH1-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33124:SF39">
        <property type="entry name" value="TRANSCRIPTION FACTOR UPBEAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9699827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="111" mass="12743" checksum="EC74E04E160CB66A" modified="2024-01-24" version="1">MGASPNPPPSALDLKGMVLQNRAKRRQWRKRSIRGGTQRRILLKRRALLGGSRRHRSDGVMRRVRTLKSLVPNGQSMALDGLFAETAYYIMWLQMRVKVMKTMVNFLSTCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A3P4</accession>
    <name>A0AA39A3P4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DUF659 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_008076</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9699299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A3P4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007021">
        <property type="entry name" value="DUF659"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008906">
        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32166:SF122">
        <property type="entry name" value="OS09G0499600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32166">
        <property type="entry name" value="OSJNBA0013A04.12 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04937">
        <property type="entry name" value="DUF659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF659" evidence="2">
        <location>
            <begin position="42"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="HAT C-terminal dimerisation" evidence="3">
        <location>
            <begin position="409"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="532"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="532"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="550"/>
            <end position="568"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9699299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="779" mass="89174" checksum="BF212A6F7DCB7115" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A8N1</accession>
    <name>A0AA39A8N1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">WRKY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_004458</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A8N1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.80:FF:000006">
        <property type="entry name" value="WRKY transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.80">
        <property type="entry name" value="WRKY domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003657">
        <property type="entry name" value="WRKY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036576">
        <property type="entry name" value="WRKY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044810">
        <property type="entry name" value="WRKY_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31221:SF322">
        <property type="entry name" value="WRKY TRANSCRIPTION FACTOR 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31221">
        <property type="entry name" value="WRKY TRANSCRIPTION FACTOR PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03106">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00774">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118290">
        <property type="entry name" value="WRKY DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50811">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="WRKY" evidence="8">
        <location>
            <begin position="133"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="WRKY" evidence="8">
        <location>
            <begin position="306"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="80"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="226"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="80"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="226"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="253"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9702739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="450" mass="49786" checksum="3401FD0B31B2DBF8" modified="2024-01-24" version="1">MADQRSQTLAPVPHPSHYDHDCPSFSDLFNGVIHSDFPTIQNVGFQGQFEMSHQEVLASVTAKAAQAPVQMDQPVCMYSSTESSSVPKSEISMPNSTPGVENPLALPQDNASIKQRADQKDFSDHKTKLADTVVMNIPNDGYNWRKYGQKQVKSTESSRSYYRCTYSDCDAKKKVQQCHQSGFVTEVIYKGFHNHDPPRKIRCTQLRKSAAVSPVEGSDTVYPTAQKLGNSDLSNYNSEPGKASVAMPELERQYSSNSDSNTGIKAEEESGDVVERKRRMKEGGLACSAPLFKTIKEPKIVVHAAGDVGISSDGYRWRKYGQKMVKGNPHPRSYYRCTSAGCPVRKHVERDTDDKTTIIVTYEGKHDHDRPVPKKRHSPRTAAFLIAAAAAMNNNQCKKTETLANQISSNQQSADIEHDLTCQKALELGGEKALESARTLLSIGIEIKPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A8W0</accession>
    <name>A0AA39A8W0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Expansin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_004779</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Causes loosening and extension of plant cell walls by disrupting non-covalent bonding between cellulose microfibrils and matrix glucans. No enzymatic activity has been found.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the expansin family. Expansin A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A8W0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009664">
        <property type="term" value="P:plant-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002963">
        <property type="entry name" value="Expansin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31867">
        <property type="entry name" value="EXPANSIN-A15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01226">
        <property type="entry name" value="EXPANSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0134">Cell wall</keyword>
    <keyword evidence="1" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0964">Secreted</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU365023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9703146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="66" mass="7251" checksum="67F04536F02C71C0" modified="2024-01-24" version="1">MPMFLKIAEYRAEIVSVAFSLVLCHEQGGIGFTINCFQYFNLVLITNVVGVGDIVRANIKRSKTGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A9W7</accession>
    <name>A0AA39A9W7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_005126</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the WUS homeobox family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A9W7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099402">
        <property type="term" value="P:plant organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044555">
        <property type="entry name" value="WUSCHEL-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45940:SF2">
        <property type="entry name" value="WUSCHEL-RELATED HOMEOBOX 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45940">
        <property type="entry name" value="WUSCHEL-RELATED HOMEOBOX 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 9 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="31"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="33"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="255"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="18"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="258"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9703698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="280" mass="31359" checksum="07E8ED43BDA4AAA8" modified="2024-01-24" version="1">MEPQQQLQQQQQQNQQQPNEDSGSSKGSFLCRQSSTRWTPTTDQIRILKDLYYNNGVRSPSAEQIQRISARLRQYGKIEGKNVFYWFQNHKARERQKKRFTTDMPMQRSLGNAGWRPDDPIHNKFHTIPTPGISSPSSSSSPSVLAVGQMGSFGYGSVERSFTDCSISAGGGRGGVGGSVNQSFEWVGMDPYSSSYALFDKRKTMGESFEEEQEEEATPEIETLPLFPMHAEDITGFCNIKPESDAYYSGWYRPADAKSSSRTSLELSLNSYAGRSPDSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AAI5</accession>
    <name>A0AA39AAI5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Chitin-binding type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_005283</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AAI5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004568">
        <property type="term" value="F:chitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016998">
        <property type="term" value="P:cell wall macromolecule catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050832">
        <property type="term" value="P:defense response to fungus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00325">
        <property type="entry name" value="chitinase_GH19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00035">
        <property type="entry name" value="ChtBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.20.10:FF:000001">
        <property type="entry name" value="Endochitinase (Chitinase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.530.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.20.10">
        <property type="entry name" value="Endochitinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.10">
        <property type="entry name" value="Endochitinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001002">
        <property type="entry name" value="Chitin-bd_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018371">
        <property type="entry name" value="Chitin-binding_1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036861">
        <property type="entry name" value="Endochitinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016283">
        <property type="entry name" value="Glyco_hydro_19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000726">
        <property type="entry name" value="Glyco_hydro_19_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22595:SF79">
        <property type="entry name" value="CHITINASE 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22595">
        <property type="entry name" value="CHITINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00187">
        <property type="entry name" value="Chitin_bind_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00182">
        <property type="entry name" value="Glyco_hydro_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001060">
        <property type="entry name" value="Endochitinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00451">
        <property type="entry name" value="CHITINBINDNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00270">
        <property type="entry name" value="ChtBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="Lysozyme-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57016">
        <property type="entry name" value="Plant lectins/antimicrobial peptides"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00026">
        <property type="entry name" value="CHIT_BIND_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50941">
        <property type="entry name" value="CHIT_BIND_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00773">
        <property type="entry name" value="CHITINASE_19_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00774">
        <property type="entry name" value="CHITINASE_19_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 7" id="KW-0147">Chitin-binding</keyword>
    <keyword evidence="4 6 7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041465747" description="Chitin-binding type-1 domain-containing protein" evidence="8">
        <location>
            <begin position="21"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-1" evidence="9">
        <location>
            <begin position="21"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="148"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6 7">
        <location>
            <begin position="24"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6 7">
        <location>
            <begin position="33"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6 7">
        <location>
            <begin position="38"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6 7">
        <location>
            <begin position="56"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="104"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="178"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="285"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001060-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001060-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9703937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="324" mass="34309" checksum="4E68842A1B061294" modified="2024-01-24" version="1">MRIWGLLLFSLLLLVGASFGQEQCGSQAGGALCSGGLCCSQYGYCGSTPAYCSTGCQSQCTSGGSPSTPSTPTPTPSGGGGDISSLISKSLFEQMLKHRNDAACPGKGFYTYEAFISAAKSFGGFGTTGDTDTRKREIAAFLAQTSHETTGGWATAPDGPYAWGYCFVQEQGNPGDYCVANQQWPCAAGKKYYGRGPIQISYNYNYGPAGRAINYDLLNNPDAVATDATISFKTALWFWMTPQSPKPSCHDVITGRWTPSSADTAAGRVPGYGVITNIINGGIECGKGSNAQVEDRIGFYKRYCDLMGIGYGNNLDCNNQSHFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AAM7</accession>
    <name>A0AA39AAM7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Aminotransferase class V domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_005376</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9704061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AAM7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43586">
        <property type="entry name" value="CYSTEINE DESULFURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43586:SF8">
        <property type="entry name" value="CYSTEINE DESULFURASE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="3">
        <location>
            <begin position="107"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="21"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9704061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="653" mass="72690" checksum="54349F60F8E470A8" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AAV9</accession>
    <name>A0AA39AAV9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Glutamate decarboxylase</fullName>
            <ecNumber evidence="3 10">4.1.1.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_004667</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-glutamate + H(+) = 4-aminobutanoate + CO2</text>
            <dbReference type="Rhea" id="RHEA:17785"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:59888"/>
            <dbReference type="EC" id="4.1.1.15"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8 9">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the group II decarboxylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.15" evidence="3 10"/>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AAV9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004351">
        <property type="term" value="F:glutamate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006538">
        <property type="term" value="P:L-glutamate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06450">
        <property type="entry name" value="DOPA_deC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000022">
        <property type="entry name" value="Glutamate decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.160:FF:000001">
        <property type="entry name" value="Glutamate decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.50:FF:000002">
        <property type="entry name" value="Glutamate decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010107">
        <property type="entry name" value="Glutamate_decarboxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01788">
        <property type="entry name" value="Glu-decarb-GAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43321">
        <property type="entry name" value="GLUTAMATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43321:SF16">
        <property type="entry name" value="GLUTAMATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="4 10" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="7 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="6 8 9" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="8">
        <location>
            <position position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR602129-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9703004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="488" mass="55280" checksum="DA8C54911E8E1B87" modified="2024-01-24" version="1">MALSETASESNASIHSTFASRYVRVSLPRFKMPTNSIPKEAAYQIINDELMLDGNPRLNLASFVTTWMEPECDKLIMASINKNYVDMDEYPVTTELQNRCVNMIAHLFNAPLGESEAAVGVGTVGSSEAIMLAGLAFKRQWQNKRKAEGKPYDKPNIVTGANVQVCWEKFARYFEVELKEVKLRDGYYVMDPVKAVELVDENTICVAAILGSTLNGEFEDVKHLNDLLTKKNQETGWDTPIHVDAASGGFIAPFLYPELEWDFRLPLVKSINVSGHKYGLVYAGIGWVIWRSKDDLPDELIFHINYLGADQPTFTLNFSKGSSQVIAQYYQLIRLGYEGYRNIMENCQENAMVLKEGLLKTGHFNIVSKDKGVPLVAFSLKDNSCHNEFEVSDMLRRYGWIVPAYTMPPDAQHVTVLRVVIREDFSRTLAERLVNDIQKVLYELDTLPAKVTAKVQESNKKSEIETQREITEAWKKFVREKKKTNGVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ACQ4</accession>
    <name>A0AA39ACQ4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Polysaccharide biosynthesis domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_003246</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ACQ4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045492">
        <property type="term" value="P:xylan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006514">
        <property type="entry name" value="IRX15/GXM/AGM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01627">
        <property type="entry name" value="A_thal_3515"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31444">
        <property type="entry name" value="OS11G0490100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21729">
        <property type="entry name" value="IRX15_IRX15L_GXM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="26"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9705106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="304" mass="33686" checksum="DEDA333226FE224A" modified="2024-01-24" version="1">MKSNGNTKLIVLHPSIHKQGGASHRLWLLFFVCFFTLAFVLTLINTAVPTPTTAASAASAHSPLPHSAMEALLHYAAMSNITGHMTAAEMASIAGVLRRCSSPCNFLIFGLTHETLLWKALNSNGRTIFLDESEFYIAKFEEKHPDIEAYDVQYTTKVSEMSELIRSAKEQLRNECRPVQNLLFSDCKLGINDLPNHLYQVDWDVILVDGPRGYFPAAPGRMSAIFTAGVLARSKKGGAAKTHVFVHDMNRDVERICSNEFLCKENLVETTDLLGHFVVETMKPNSFRFCRNPTKSSSPSSSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ADA9</accession>
    <name>A0AA39ADA9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_003258</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ADA9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000400">
        <property type="entry name" value="Nuclear pore complex protein NUP107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053213">
        <property type="entry name" value="RLP29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48009">
        <property type="entry name" value="LEUCINE-RICH REPEAT (LRR) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48009:SF9">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="63"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="9">
        <location>
            <begin position="90"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="10">
        <location>
            <begin position="159"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9705124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="446" mass="48250" checksum="0CFA156A1A81A556" modified="2024-01-24" version="1">MRVVRKWSPTGQRPCTTDIPILISKTHAEAQGPTHFPTPPLQRPLLLFQIQKGDREVMPLQQFLALLAMSHLSLLLLFSVFSATHALTLPSDVSALQSFKASIKPSSVSPWSCLASWNFFTDPCSVPRRTHFTCGISCSADSTRVISITLDPAGYAGTLSPAIGKLTQLTVLDLSDNSFSGYVPSALSSLSNLQILTLRSNSFSGPLPQAITAIKSLESLDISHNFLSGSLPKTIGSLSSLRRLDLSFNRITGTLPKLPSSLSELALRSNSLSGYLLKSSFDGLTRLEVVELSANAFTGPIQSWFFLLPSLQQVNLANNSFTGLEILKPTSSDLVAVDLGFNRIKGYIPTNFPAFPLLSSLSLRYNQLRGPIPMDYSKKETLKRLFLDGNFLNGKAPVGFFSGWSGVSGSLGDNCLQSCPTSSQLCLPSQKPNSICKQAYGGKPRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ADQ4</accession>
    <name>A0AA39ADQ4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MULE transposase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_003617</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ADQ4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018289">
        <property type="entry name" value="MULE_transposase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31973:SF195">
        <property type="entry name" value="MUDR FAMILY TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31973">
        <property type="entry name" value="POLYPROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10551">
        <property type="entry name" value="MULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MULE transposase" evidence="1">
        <location>
            <begin position="414"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9705632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="780" mass="90739" checksum="299475CE3B22AB24" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AEJ1</accession>
    <name>A0AA39AEJ1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_001631</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the PP2C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AEJ1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000041">
        <property type="entry name" value="Protein phosphatase 2C 51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0464">Manganese</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="11">
        <location>
            <begin position="101"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="25"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="27"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="87"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9706170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="393" mass="42476" checksum="85C01BBC9583ED68" modified="2024-01-24" version="1">MDSVETIKPRVDPQLGADAYVKKRRTGIKEESSFRSSKGVEEESKMDDGAVKLNSELSTSGSSAPESSASQSGGVSDGAVVSEGEEQGGKEGEKRASTCVPHGSMSVIGRRRAMEDALTVAPGEFASYDFYAVYDGHGGAKVAYACRDRLHRLLAKEIEDAIDGEGRIDWENVMVASFSKMDEEINGEANQGEDRSTSSLLRSMGSTAVVVVVGPEKLVVANCGDSRAVLCRRGVAVPLSRDHKPDRPDERERVEAAGGKVINWNGFRILGVLSTSRSIGDYFLKPYVTPKPEVTVWEREEFDDFIVIASDGLWDVITNELACKIVRRCFDGQIRRRVSEGMSGSCAAEAAAMLTELAMAQGSKDNISVVVVELKKHLCHGGLKLKNPNSPSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AG63</accession>
    <name>A0AA39AG63_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Expansin-like B1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_002146</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the expansin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AG63"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.760">
        <property type="entry name" value="Expansin, cellulose-binding-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007118">
        <property type="entry name" value="Expan_Lol_pI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007112">
        <property type="entry name" value="Expansin/allergen_DPBB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007117">
        <property type="entry name" value="Expansin_CBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036749">
        <property type="entry name" value="Expansin_CBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009009">
        <property type="entry name" value="RlpA-like_DPBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31692">
        <property type="entry name" value="EXPANSIN-B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31692:SF2">
        <property type="entry name" value="EXPANSIN-LIKE B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03330">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01357">
        <property type="entry name" value="Expansin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01225">
        <property type="entry name" value="EXPANSNFAMLY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49590">
        <property type="entry name" value="PHL pollen allergen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50843">
        <property type="entry name" value="EXPANSIN_CBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50842">
        <property type="entry name" value="EXPANSIN_EG45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041420748" description="Expansin-like B1" evidence="2">
        <location>
            <begin position="25"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like EG45" evidence="3">
        <location>
            <begin position="46"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like CBD" evidence="4">
        <location>
            <begin position="163"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9707028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="251" mass="27370" checksum="B1FC1F2EA0547E6F" modified="2024-01-24" version="1">MGFPPSHYCCFLCVIVLLPVLCKGENTFTYSLATYYGSADGLGTPTGACGFGEYGRSVNGGNVAAVSRLYRDGTGCGACYQVRCNMPNLCADDGIEVVVTDQGGGDNTAFILSKRGFSMLALPNRAADLIPYGMVGIEYRRVPCHYPDSNIFFKVHEHSRFPDYLAIVILYQAGLSDITSVNIWKEDSQAWRCMGNSHGAVWDMSNPPKGPVSLRLQVSRGGGQKWVQLMNVIPGDWKAGVAYDSLFQLDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AGY6</accession>
    <name>A0AA39AGY6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Cation/H+ exchanger domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_001581</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the monovalent cation:proton antiporter 2 (CPA2) transporter (TC 2.A.37) family. CHX (TC 2.A.37.4) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AGY6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006885">
        <property type="term" value="P:regulation of pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1530.20:FF:000003">
        <property type="entry name" value="Cation/H(+) antiporter 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057291">
        <property type="entry name" value="CHX17_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057290">
        <property type="entry name" value="CHX17_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050794">
        <property type="entry name" value="CPA2_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32468">
        <property type="entry name" value="CATION/H + ANTIPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32468:SF144">
        <property type="entry name" value="CATION_H(+) ANTIPORTER 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23256">
        <property type="entry name" value="CHX17_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23259">
        <property type="entry name" value="CHX17_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0050">Antiport</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0630">Potassium</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="42"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="79"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="108"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="142"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="176"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="206"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="247"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="325"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="389"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="13">
        <location>
            <begin position="29"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H(+) antiporter central" evidence="14">
        <location>
            <begin position="465"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H(+) antiporter C-terminal" evidence="15">
        <location>
            <begin position="611"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="652"/>
            <end position="671"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF23256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF23259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9706079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="771" mass="83179" checksum="8BC66F08BA9222E1" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AI03</accession>
    <name>A0AA39AI03_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_000149</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Involved in the targeting and/or fusion of transport vesicles to their target membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Early endosome membrane</location>
            <topology evidence="11">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the synaptobrevin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9707942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AI03"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14824">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15843">
        <property type="entry name" value="R-SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000010">
        <property type="entry name" value="Vesicle-associated membrane protein 726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.50:FF:000014">
        <property type="entry name" value="vesicle-associated membrane protein 727"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.50">
        <property type="entry name" value="Longin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010908">
        <property type="entry name" value="Longin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001388">
        <property type="entry name" value="Synaptobrevin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051097">
        <property type="entry name" value="Synaptobrevin-like_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21136:SF92">
        <property type="entry name" value="LONGIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21136">
        <property type="entry name" value="SNARE PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13774">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00219">
        <property type="entry name" value="SYNAPTOBREVN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01270">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50859">
        <property type="entry name" value="LONGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="194"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="Longin" evidence="14">
        <location>
            <begin position="10"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="15">
        <location>
            <begin position="130"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9707942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="221" mass="24915" checksum="223A78B05403346B" modified="2024-01-24" version="1">MGQKSLIYAFVARGTVILAEYTEFSGNFNSIAFQCLQKLPATSNKFTYNCDAHTFTYLLDNGYTYCVVADESVGRQVPMAFLERIRDDFVSRYGGEKAATAPANSLNKDFSSKLKEHMQYCVDHPEEISKLAKVKDQVSEVKGVMMENIEKVLDRGEKIELLVDKTQNLQEQAQDFRSAGTKIRRKMWLQNMKIKLIVLGILVALILIIVLSVCHGLNCGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AJ90</accession>
    <name>A0AA39AJ90_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Importin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_000208</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the importin beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AJ90"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000459">
        <property type="entry name" value="ARM repeat superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056840">
        <property type="entry name" value="HEAT_IPO9_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058669">
        <property type="entry name" value="TPR_IPO7/11-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997">
        <property type="entry name" value="IMPORTIN-7, 8, 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997:SF9">
        <property type="entry name" value="IMPORTIN-9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25018">
        <property type="entry name" value="HEAT_IPO9_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25758">
        <property type="entry name" value="TPR_IPO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="6">
        <location>
            <begin position="27"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9708035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1016" mass="112384" checksum="DBF75E2420434079" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AK90</accession>
    <name>A0AA39AK90_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CCZ1/INTU/HSP4 first Longin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_000558</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CCZ1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AK90"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035658">
        <property type="term" value="C:Mon1-Ccz1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013176">
        <property type="entry name" value="Ccz1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043987">
        <property type="entry name" value="CCZ1/INTU/HSP4_longin_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13056">
        <property type="entry name" value="VACUOLAR FUSION PROTEIN CCZ1 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13056:SF0">
        <property type="entry name" value="VACUOLAR FUSION PROTEIN CCZ1 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19031">
        <property type="entry name" value="Intu_longin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCZ1/INTU/HSP4 first Longin" evidence="2">
        <location>
            <begin position="16"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9708582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="528" mass="59184" checksum="92ECE554145D29D6" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AL37</accession>
    <name>A0AA39AL37_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_000911</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AL37"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10747">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.20:FF:000030">
        <property type="entry name" value="DNAJ heat shock family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.20:FF:000002">
        <property type="entry name" value="Dnaj homolog subfamily b member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.260.20">
        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051339">
        <property type="entry name" value="DnaJ_subfamily_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24078:SF524">
        <property type="entry name" value="DNAJ HEAT SHOCK FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24078">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="HSP40/DnaJ peptide-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="4"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9709174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="274" mass="31254" checksum="4AD0F492635C3CB3" modified="2024-01-24" version="1">MAIDYYKLLRVNQNASAEELKKAYKRLAIKWHPDKNPNHNRVEAEAKFKQISEAYDVLSDSRKRQIYDLYGNQYASSFNHRDARDVFAELFGSGSKAMEKRLDCSLEELYQGSKREIKISRTVIRESGKVRIVEETLLITIEPGWKKGTKITFPMKGNQEPGMAPSDLIFVVHEKPHALYEREGNDLVVKQSISLLEALTGKTLILTTLDGRNLTIPVTDIVRPGYVMVIPDEGMPMSKEPTKKGNLRIKFDVKFPPRLTAQQKYEVKRVLVGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ALC6</accession>
    <name>A0AA39ALC6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_001318</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the DEAD box helicase family. DEAH subfamily. PRP16 sub-subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000007">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000615">
        <property type="entry name" value="pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000018">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase prp16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF91">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR ATP-DEPENDENT RNA HELICASE PRP16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
        <location>
            <begin position="601"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="15">
        <location>
            <begin position="786"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="104"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1220"/>
            <end position="1289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="137"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="148"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="166"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="226"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="288"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="311"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1220"/>
            <end position="1263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9709800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1289" mass="145101" checksum="FA49BED185ACB0F9" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D4W1</accession>
    <name>A0AA39D4W1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_027281</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9671216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D4W1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051222">
        <property type="entry name" value="PPR/CCM1_RNA-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47942:SF15">
        <property type="entry name" value="OS12G0557800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47942">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT (TPR)-LIKE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12854">
        <property type="entry name" value="PPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="181"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="251"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="286"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="321"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="356"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="391"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="426"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="461"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="496"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="531"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="566"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="601"/>
            <end position="635"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9671216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="659" mass="74469" checksum="2358FD8B0BEB1265" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D5M4</accession>
    <name>A0AA39D5M4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_025592</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D5M4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22157">
        <property type="entry name" value="F-box_AtFBW1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013187">
        <property type="entry name" value="F-box-assoc_dom_typ3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017451">
        <property type="entry name" value="F-box-assoc_interact_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011043">
        <property type="entry name" value="Gal_Oxase/kelch_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050796">
        <property type="entry name" value="SCF_F-box_component"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01640">
        <property type="entry name" value="F_box_assoc_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31672">
        <property type="entry name" value="BNACNNG10540D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31672:SF13">
        <property type="entry name" value="F-BOX PROTEIN CPR30-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08268">
        <property type="entry name" value="FBA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50965">
        <property type="entry name" value="Galactose oxidase, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="21"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9672011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="368" mass="42974" checksum="3A17EB210B8AB86C" modified="2024-01-24" version="1">MDDFGSKLMRVTQSENVFEVEEILTNILSRLPVKSLLVCKSVCKYWQGLICSPSFIHLHLIQSQENPSYVFCPSYVFWDNNAHWVIKTDGETTQSFPVYDGFYFRDMICSFNGLICCTTPRKSGSCIIRRSPRRGMDIIIYNPATRKVLSLPPTPKTHYAPKLGVSFGPTIDGYEIFQFFLIREEQLYECMVYSSITGSWEFIGTVAHAPFNSFNHVCIDGIVYWFSRSRIDGRLVGHILTVDREEKFSIIRIPEEETLYPLLVNLEGCLCLVAENGLDQNRFDIWALQNSKESVWVKKWSDYMPSFKIEQLQYIVVRKNEILFGSWKQYLFYNMGTRTWREFNWEYGRENGFSFPLAYTESLLSCKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D5V8</accession>
    <name>A0AA39D5V8_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_027202</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9671095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D5V8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000323">
        <property type="term" value="C:lytic vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035493">
        <property type="term" value="P:SNARE complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15157:SF25">
        <property type="entry name" value="OS07G0418000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15157">
        <property type="entry name" value="UV RADIATION RESISTANCE-ASSOCIATED GENE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9671095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="268" mass="30524" checksum="747E3BBFFB2C5DEB" modified="2024-01-24" version="1">MGDLYALDFDGILCDSCGESSLSAVKAAKVRWPGLFDGVDSKLEDWIVDQMFIIRPVVETGYENLLLVRLLLEMRLPSIRKSSVAEVLTIEGILENWSKLKPVIMEEWDEKREPLVDLFGKVRDEWMEDDLATWIDANRFYPGVADALKFASSKVYIVTTKQGRFAEALLRELAGVMIPSERIYGLGTGPKVEVLKQLQKQSEHQGLTLHFVEDRLATLKNVIKEPELDGWNLYLGKWGYNTQKEREEAAGISRIQLLELSDFSKKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D775</accession>
    <name>A0AA39D775_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Diacylglycerol kinase</fullName>
            <shortName evidence="8">DAG kinase</shortName>
            <ecNumber evidence="8">2.7.1.107</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_025466</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a 1,2-diacyl-sn-glycerol + ATP = a 1,2-diacyl-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10272"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.107"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the eukaryotic diacylglycerol kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.107" evidence="8"/>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D775"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004143">
        <property type="term" value="F:ATP-dependent diacylglycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10330:FF:000016">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037607">
        <property type="entry name" value="DGK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000756">
        <property type="entry name" value="Diacylglycerol_kin_accessory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001206">
        <property type="entry name" value="Diacylglycerol_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11255">
        <property type="entry name" value="DIACYLGLYCEROL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11255:SF29">
        <property type="entry name" value="DIACYLGLYCEROL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00609">
        <property type="entry name" value="DAGK_acc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00781">
        <property type="entry name" value="DAGK_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00045">
        <property type="entry name" value="DAGKa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00046">
        <property type="entry name" value="DAGKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50146">
        <property type="entry name" value="DAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0346">Stress response</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DAGKc" evidence="10">
        <location>
            <begin position="32"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="449"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9671767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="479" mass="53785" checksum="D359A2D24B146138" modified="2024-01-24" version="1">MDNSKSENILKDFYIPNYILLPESEVRNASHIPSSPVLVFINSKSGGQLGGELLCTYGALLNKNQVFDLDNEGPDKVLHQFYFNLEKLKHSGDILASEIQNRLRIIVAGGDGTANWLLGVVSDLKLAQPPPIAMVPLGTGNNLPFSFGWGKRNPGSDHQSVESFLDQVRTAREMKIDSWHITLRVRPPEEGSYDPTLELPHSLHAFQHVYPTKKPNMEGCCTFQGGFWNYFSVGMDAHVSYAFHSERKLHPEKFKNQLVNQSIYAKLACKEGLFCTSLLYPSSWNIAKLTKVRIMKKQGQWEDLPIPCSIRSIICLNLPSFSGGLNPWGMPSRKALHNRDFTPLFVDDGIIEVVGFRDAWHGLCLYVPRGHGTRLAQVKRIRFDFHHSAANHVFMRIDGEPWKQPLPVDDNPVVVEISHLGQVTVLATPHCRSTSITESSSLVSHVDIKEDSSETEDSDMPRKFGAADTFRMSDDHLMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D7V6</accession>
    <name>A0AA39D7V6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cellulose synthase-like protein E1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_025871</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Thought to be a Golgi-localized beta-glycan synthase that polymerize the backbones of noncellulosic polysaccharides (hemicelluloses) of plant cell wall.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D7V6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016760">
        <property type="term" value="F:cellulose synthase (UDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030244">
        <property type="term" value="P:cellulose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000138">
        <property type="entry name" value="Cellulose synthase isolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005150">
        <property type="entry name" value="Cellulose_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13301">
        <property type="entry name" value="X-BOX TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03552">
        <property type="entry name" value="Cellulose_synt"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="505"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="543"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="591"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="631"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="665"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="320"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR605150-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR605150-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9672427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="685" mass="78357" checksum="8E97B69D061059E7" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D8D2</accession>
    <name>A0AA39D8D2_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_024524</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D8D2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47292:SF3">
        <property type="entry name" value="PROTEIN WAVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47292">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR (TFIIS) FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="215"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="560"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="217"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9675638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="903" mass="97433" checksum="5411A9B76ACBFA3F" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D916</accession>
    <name>A0AA39D916_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glycosyltransferase</fullName>
            <ecNumber evidence="2">2.4.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_023914</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the glycosyltransferase 77 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.-" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D916"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080147">
        <property type="term" value="P:root hair cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005069">
        <property type="entry name" value="Nucl-diP-sugar_transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044290">
        <property type="entry name" value="RRA1/2/3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46581">
        <property type="entry name" value="ARABINOSYLTRANSFERASE RRA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46581:SF3">
        <property type="entry name" value="ARABINOSYLTRANSFERASE RRA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03407">
        <property type="entry name" value="Nucleotid_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="2" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleotide-diphospho-sugar transferase" evidence="4">
        <location>
            <begin position="189"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="75"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9674665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="434" mass="49276" checksum="0AEBD4B9503ED2A1" modified="2024-01-24" version="1">MAGRREGLLTRNSNGNSLRGSRIAVAITIGVLLGCVFAFLYPHGLFRSDPQITNPRFVKSNLQVGSPSCESPERLKMLKSDIVALSEKNADLKKQVRELTEKLRLAEQGKDQAQKQFMVLGEQHKAGPFGTVKSLRTNPTLIPDDSVNPRLAKILEEVAVRKELIVALANSNVKSSLAVWFANIKRVGIPNYLVVALDDDIENFCKSNNVPVYKRDPDEDIDSVARTGGNHAVSGLKFRILREFLQLGYSVLLSDIDIVYLQNPFEYLYRDSDVESMTDGHNNYTAYGYNDVFDEPAMGWARYAHTMRIWVYNSGFFYIRPTIPSIELLDRVADRLAHSKAWDQAVFNEELFFPSHPGYTGLHASRRTMDFYLFMNSKVLFKTVRKDAKLKKLKPVIVHVNYHPDKLSRMEAVVEYYVNGKQDALDRFPDGSNW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D9B6</accession>
    <name>A0AA39D9B6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase Ty1/copia-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_024623</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D9B6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase Ty1/copia-type" evidence="1">
        <location>
            <begin position="29"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9675776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="164" mass="18678" checksum="A74C8E2A63C7B2DA" modified="2024-01-24" version="1">MITRSKLKNDPSLKSQMVTFAATRSDITPRARFDKLSQCLLHLGFYCGKADSSLFILRKGQSIVLLLIYVDDIIVTGNDNNIISDLISTLSSEFSLKDLRSLHYFLGLEVKYLPNGLFVSQTKYTRDLLEHTKMMECTHINTPMALKSIITLSDEQPINPTRYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DAS6</accession>
    <name>A0AA39DAS6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">F-box/LRR-repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_021901</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9676615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DAS6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032436">
        <property type="term" value="P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019494">
        <property type="entry name" value="FIST_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14939">
        <property type="entry name" value="F-BOX ONLY PROTEIN 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14939:SF5">
        <property type="entry name" value="F-BOX ONLY PROTEIN 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10442">
        <property type="entry name" value="FIST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01204">
        <property type="entry name" value="FIST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="12"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="FIST C-domain" evidence="2">
        <location>
            <begin position="321"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9676615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="527" mass="57730" checksum="0986FA4FEDC794D6" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DB85</accession>
    <name>A0AA39DB85_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">MADS-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_022433</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DB85"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010152">
        <property type="term" value="P:pollen maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080092">
        <property type="term" value="P:regulation of pollen tube growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1810.10:FF:000010">
        <property type="entry name" value="Agamous-like MADS-box protein AGL30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050142">
        <property type="entry name" value="MADS-box/MEF2_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48019">
        <property type="entry name" value="SERUM RESPONSE FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00404">
        <property type="entry name" value="MADSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="7">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="77"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9677439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="375" mass="42465" checksum="7FCD48D26B2A202C" modified="2024-01-24" version="1">MGRVKLKIKRLESTSNRQVTYSKRRNGILKKARELSILCDIEIILLMFSPTGRPTLFHGARSNIEEVIAKFAQLTPQERAKRKLESLEALKKTFKKLDHDVNLQDFLGASTQTIEELTNQARLLQAQLTEVHKRLSYWSNPDKVDSTEHLRQMEDSLRESLNRIRVHKENFGKHQLMSLECASQFQNGMHLPLIMDGVQEAQPLSWLPNNENQHLILPEEPSYLPQRDIECSADASIPGYSGYYSTGKQTEIGNSGQVDEQGQEGAALNQLSGNSNLRLQLSEQYLYSPFGNLNLPDEKKLKPEMEMNLQGNPVDYQVNGNFEIPAPIYDNRQHTWVSASGPCSIAMFDENSFSQGNIPPIVHSAFPDSQQNSLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DDE1</accession>
    <name>A0AA39DDE1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_021505</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DDE1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000111">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046956">
        <property type="entry name" value="RLP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063">
        <property type="entry name" value="LRR RECEPTOR-LIKE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063:SF101">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE FLS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041402507" description="Leucine-rich repeat-containing N-terminal plant-type domain-containing protein" evidence="13">
        <location>
            <begin position="25"/>
            <end position="1029"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="980"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="14">
        <location>
            <begin position="39"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="15">
        <location>
            <begin position="105"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9679600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1029" mass="113878" checksum="AE29FEAB4D005D31" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DDF2</accession>
    <name>A0AA39DDF2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_021758</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DDF2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000014">
        <property type="entry name" value="GTPase-activating protein gyp7 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000022">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957:SF507">
        <property type="entry name" value="OS08G0547200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="34"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="Rab-GAP TBC" evidence="3">
        <location>
            <begin position="104"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="171"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="256"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="195"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9679961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="552" mass="61763" checksum="82A63B630C637197" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DFL5</accession>
    <name>A0AA39DFL5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein ECERIFERUM 1-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_019655</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sterol desaturase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DFL5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021940">
        <property type="entry name" value="CER1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006694">
        <property type="entry name" value="Fatty_acid_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050307">
        <property type="entry name" value="Sterol_Desaturase_Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11863">
        <property type="entry name" value="STEROL DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12076">
        <property type="entry name" value="CER1-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04116">
        <property type="entry name" value="FA_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="19"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="99"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="127"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="184"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="230"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="329"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid hydroxylase" evidence="7">
        <location>
            <begin position="133"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="Very-long-chain aldehyde decarbonylase CER1-like C-terminal" evidence="8">
        <location>
            <begin position="451"/>
            <end position="614"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9680392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="619" mass="72260" checksum="045113691BD9BD3F" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DGH4</accession>
    <name>A0AA39DGH4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_018921</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DGH4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF78">
        <property type="entry name" value="OS12G0587133 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9683124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="622" mass="71793" checksum="0C3AA0166FB1F66C" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DHY2</accession>
    <name>A0AA39DHY2_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_018672</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DHY2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038582">
        <property type="entry name" value="Ribosomal_eS31_euk-type_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9682792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="101" mass="11317" checksum="508413DFDA1B6BB1" modified="2024-01-24" version="1">MSSGVLCVAQRIVSSMEQKEEGCQNDDHVSLAKDYIIQEDSTLYLVLRLRGSVKKCKKKAKPAILQSYKVDDSGKIKRLRNERPNSECVNACNLGEVESQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DJC9</accession>
    <name>A0AA39DJC9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_017667</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DJC9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36616">
        <property type="entry name" value="BNAC07G32700D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36616:SF4">
        <property type="entry name" value="OS03G0174800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041460399" description="Secreted protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9685720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="67" mass="7972" checksum="C0E019899F613E45" modified="2024-01-24" version="1">MLQLFFAVAFSSVPLTLYIPPLRSLNLFVETMEDIVRETSMHTDRLYPRVRQAFFRILDCMLCNNMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DNA6</accession>
    <name>A0AA39DNA6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_013208</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DNA6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009791">
        <property type="term" value="P:post-embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000453">
        <property type="entry name" value="Leucine-rich receptor-like protein kinase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000722">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000233">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase TDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001387">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase FLS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001009">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase FLS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000676">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase FLS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000299">
        <property type="entry name" value="Piriformospora indica-insensitive protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27000">
        <property type="entry name" value="LEUCINE-RICH REPEAT RECEPTOR-LIKE PROTEIN KINASE FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27000:SF616">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE FLS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 15" id="KW-0732">Signal</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041409068" description="Protein kinase domain-containing protein" evidence="15">
        <location>
            <begin position="24"/>
            <end position="1168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="808"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="871"/>
            <end position="1157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9690941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1168" mass="127067" checksum="AA4C09E4421CC1EA" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DNN1</accession>
    <name>A0AA39DNN1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_013561</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DNN1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000438">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025724">
        <property type="entry name" value="GAG-pre-integrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054722">
        <property type="entry name" value="PolX-like_BBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039537">
        <property type="entry name" value="Retrotran_Ty1/copia-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057670">
        <property type="entry name" value="SH3_retrovirus"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648">
        <property type="entry name" value="TRANSPOSASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648:SF28">
        <property type="entry name" value="TRANSPOSON-ENCODED PROTEIN WITH RIBONUCLEASE H-LIKE AND RETROVIRUS ZINC FINGER-LIKE DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13976">
        <property type="entry name" value="gag_pre-integrs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22936">
        <property type="entry name" value="Pol_BBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25597">
        <property type="entry name" value="SH3_retrovirus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="780"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase catalytic" evidence="4">
        <location>
            <begin position="363"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="81"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="81"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9691418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="814" mass="90256" checksum="465BC1E2532B6A12" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DQF4</accession>
    <name>A0AA39DQF4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pseudouridine synthase RsuA/RluA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_012135</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9693263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DQF4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02869">
        <property type="entry name" value="PseudoU_synth_RluA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.10">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050188">
        <property type="entry name" value="RluA_PseudoU_synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21600">
        <property type="entry name" value="MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21600:SF53">
        <property type="entry name" value="RNA PSEUDOURIDINE SYNTHASE 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pseudouridine synthase RsuA/RluA-like" evidence="1">
        <location>
            <begin position="188"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9693263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="479" mass="53805" checksum="753E2688480051B2" modified="2024-01-24" version="1">MWKNVLHECQGLFLVVRHYSRIAPPRPASAAPVIRVSNNVAQLGSPKEGPKPRQLLSLPPFPAHPLPGKSLVNVSGLPTHVTAISWIKYYFDEIPGFVVQSHFNKGLVHMECSNLDDSPLEKEGQIKPLRKIKPNEVMEAGTRIYVPVSVAEARISKRFDTIPSGTLHPNADEIEYLQRLVMYKDPAILVLNKPPKVPLKGNLPVHNSMDALAAAALTFDYEEGPKLVHRLDRESSGLLLMGRTKESVAHLHWLFSDINKAKSPSKAWDGACEATCQRYWALVIGSPKQKEGVIHAPLSKVLLDDGRTERVILANQSGLEASQEAITEYRVLGPMINGCSWIELCPRTSRKHQLRVHSAEALGTPIVGDYKYGWFVHRRWKQMPRVDLEPTTGKPYKMRRPEGLDVQKGSVLSKVPLLHLHCRELILPNIAKFLPILDLKPENQNQYPKLSSKPDILRFVASMPNHMKISWNLMSSYLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DX70</accession>
    <name>A0AA39DX70_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_005849</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DX70"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35124">
        <property type="entry name" value="CYTOCHROME P450 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35124:SF1">
        <property type="entry name" value="CYTOCHROME P450 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9700223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="627" mass="71606" checksum="F00D6A700817407D" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DY24</accession>
    <name>A0AA39DY24_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">Sucrose synthase</fullName>
            <ecNumber evidence="2 6">2.4.1.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_005495</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Sucrose-cleaving enzyme that provides UDP-glucose and fructose for various metabolic pathways.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>an NDP-alpha-D-glucose + D-fructose = a ribonucleoside 5'-diphosphate + sucrose + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16241"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17992"/>
            <dbReference type="ChEBI" id="CHEBI:37721"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:76533"/>
            <dbReference type="EC" id="2.4.1.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the glycosyltransferase 1 family. Plant sucrose synthase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.13" evidence="2 6"/>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9699655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DY24"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016157">
        <property type="term" value="F:sucrose synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005985">
        <property type="term" value="P:sucrose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03800">
        <property type="entry name" value="GT4_sucrose_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1230:FF:000001">
        <property type="entry name" value="Sucrose synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.330:FF:000001">
        <property type="entry name" value="Sucrose synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000006">
        <property type="entry name" value="Sucrose synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000549">
        <property type="entry name" value="Sucrose synthase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000368">
        <property type="entry name" value="Sucrose_synth_GT-B1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012820">
        <property type="entry name" value="Sucrose_synthase_pln/cyn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056736">
        <property type="entry name" value="SUS_EPBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056735">
        <property type="entry name" value="SUS_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02470">
        <property type="entry name" value="sucr_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45839">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45839:SF9">
        <property type="entry name" value="SUCROSE SYNTHASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00862">
        <property type="entry name" value="GT-B_Sucrose_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24862">
        <property type="entry name" value="SUS_EPBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24861">
        <property type="entry name" value="SUS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sucrose synthase N-terminal" evidence="9">
        <location>
            <begin position="8"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Sucrose synthase EPBD" evidence="10">
        <location>
            <begin position="155"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="Sucrose synthase first GT-B" evidence="8">
        <location>
            <begin position="265"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="7">
        <location>
            <begin position="566"/>
            <end position="728"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU280817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF24861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF24862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9699655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="808" mass="92531" checksum="20FEFB3C76FD716F" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2025-06-18" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DZ49</accession>
    <name>A0AA39DZ49_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_008014</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9699202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15532">
        <property type="entry name" value="PHD2_CHD_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15489">
        <property type="entry name" value="PHD_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20401">
        <property type="entry name" value="Tudor_AtPTM-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056618">
        <property type="entry name" value="Chromo_PTM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018501">
        <property type="entry name" value="DDT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047365">
        <property type="entry name" value="Tudor_AtPTM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46508">
        <property type="entry name" value="PHD FINGER FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46508:SF5">
        <property type="entry name" value="PHD-FINGER AND DNA BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24294">
        <property type="entry name" value="Chromo_PTM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02791">
        <property type="entry name" value="DDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21743">
        <property type="entry name" value="PTM_DIR17_Tudor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00571">
        <property type="entry name" value="DDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50827">
        <property type="entry name" value="DDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DDT" evidence="9">
        <location>
            <begin position="396"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="8">
        <location>
            <begin position="592"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="67"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="70"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="90"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9699202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1704" mass="188314" checksum="7CDE37309117AD1D" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DZK3</accession>
    <name>A0AA39DZK3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WAT1-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_005301</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the drug/metabolite transporter (DMT) superfamily. Plant drug/metabolite exporter (P-DME) (TC 2.A.7.4) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DZK3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030184">
        <property type="entry name" value="WAT1-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31218">
        <property type="entry name" value="WAT1-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="102"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="188"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="219"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="257"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="282"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="309"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="17"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="189"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9703961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="399" mass="43106" checksum="74D966E34CCBEF2A" modified="2024-01-24" version="1">MATGKLRNLLEGLKPAMTMVIVQIIFGGLNIMYKLARNDGMSMKILVAYRNISATAIMVPLALIFEGKSRPKLTWMIFLQGSLCGLFGGSLGQNLYAESLNLTSATFVAAMTNLVPAMTFVMAVFLRMERLAIGTIAGKAKVLGTIMSLGGAMILTFYKGVEIKLWSTNINLLHHGAAALQEPSTNQVLGSLLAVAGCVCIAVWLIVQTKMSMVYPSYSGTALMCVCASIQSVVYAMCTERDWSAWKLGWNIRLLTVVYSGVLASGLIVTLIAWVSRMRGPLFVSSFFPLMLVTVAILGSLLLHEQLHIGSIIAAVLIIVGLYIVLWGKGKEMKQTAQIHAAQSFREQDLRHIVIENSSNSECKPKASGIIGPIEPADLGPGLTISPTANIEIHLQCLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DZQ3</accession>
    <name>A0AA39DZQ3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 15">Peroxidase</fullName>
            <ecNumber evidence="4 15">1.11.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PVL29_004496</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:56136"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:139520"/>
            <dbReference type="ChEBI" id="CHEBI:139521"/>
            <dbReference type="EC" id="1.11.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12 15">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="12 15">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12 15">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="12 15">Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peroxidase family. Ascorbate peroxidase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.7" evidence="4 15"/>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DZQ3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140825">
        <property type="term" value="F:lactoperoxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00693">
        <property type="entry name" value="secretory_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.420.10:FF:000001">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.420.10">
        <property type="entry name" value="Peroxidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000823">
        <property type="entry name" value="Peroxidase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019793">
        <property type="entry name" value="Peroxidases_heam-ligand_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033905">
        <property type="entry name" value="Secretory_peroxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31517">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31517:SF17">
        <property type="entry name" value="PEROXIDASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00458">
        <property type="entry name" value="PEROXIDASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00461">
        <property type="entry name" value="PLPEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00435">
        <property type="entry name" value="PEROXIDASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50873">
        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 15" id="KW-0106">Calcium</keyword>
    <keyword evidence="10 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 15" id="KW-0349">Heme</keyword>
    <keyword evidence="15" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="9 12 15" id="KW-0408">Iron</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 12 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5 15" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0964">Secreted</keyword>
    <keyword evidence="15" id="KW-0732">Signal</keyword>
    <keyword evidence="16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041489465" description="Peroxidase" evidence="15">
        <location>
            <begin position="30"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="141"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="Plant heme peroxidase family profile" evidence="17">
        <location>
            <begin position="30"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="71"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="12">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="280"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="13">
        <location>
            <position position="67"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="40"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="73"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="122"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="230"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU362060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ9702788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="358" mass="39198" checksum="66F0C492744C5645" modified="2024-01-24" version="1">METKLLVFSPKLLILLFFSFAAFPSPSSSSLAFNFYGASCPSAEFIVKNTVRSASSFDPTIPGKLLRLLFHDCMVEGCDASVLLQGNDTERSDPANASLGGFSVINSAKRVLEIFCPGTVSCADILALAARDAVEIVPSPSLFLLWIFFSLPFLVGYLVMKYWEQVGGPMLQIPTGRRDGRASVASVVRFNIIDTSFSMDEMMKLFSSKGLSLDDLVILSGAHTIGSAHCSAFSDRFQADSKGKLTSIDASLDKAYANELRKKCPAGVSSSVTVNNDPETSFSFDNQYYRNLMAHKGLFQSDSVLFNDKRTKKMVEDLANDQNSFFERWGQSFLKLTIIGVKSDDEGEIRQSCEVANG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E0M6</accession>
    <name>A0AA39E0M6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">PXMP2/4 family protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_003324</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the peroxisomal membrane protein PXMP2/4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E0M6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007248">
        <property type="entry name" value="Mpv17_PMP22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266:SF18">
        <property type="entry name" value="OS12G0508100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04117">
        <property type="entry name" value="Mpv17_PMP22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9705206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="247" mass="27731" checksum="E4172E0ABBC880E9" modified="2024-01-24" version="1">MRGVFLKNGLVHRFSQICKSADTKNPFLIPRDRATRDNHHYHNFLNKSRESASGAFSIPFSSASSSSRSRIGFVGWYLGLVHSRPLLTKSVTSSLIYAAADCTSQTISRQSSESYDFMRTLRMAGYGMLILGPSLHFWFNFISKVLPQRDIITTLKKICLGQTTFGPFMTAIFFSANAAVQGENGSDIIARLNRDLIPTLINGVMYWPICDFVTFKFIPCPFTGMCKVSVIHIKLRLINCGWVYSEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E117</accession>
    <name>A0AA39E117_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Dynamin-related protein 3A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_003611</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E117"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08771">
        <property type="entry name" value="DLP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1240:FF:000013">
        <property type="entry name" value="Dynamin-related protein 3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1240:FF:000018">
        <property type="entry name" value="Dynamin-related protein 3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001027">
        <property type="entry name" value="dynamin-related protein 3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1240">
        <property type="entry name" value="Dynamin, middle domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022812">
        <property type="entry name" value="Dynamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001401">
        <property type="entry name" value="Dynamin_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019762">
        <property type="entry name" value="Dynamin_GTPase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000375">
        <property type="entry name" value="Dynamin_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030381">
        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003130">
        <property type="entry name" value="GED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020850">
        <property type="entry name" value="GED_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566">
        <property type="entry name" value="DYNAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566:SF21">
        <property type="entry name" value="DYNAMIN RELATED PROTEIN 1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01031">
        <property type="entry name" value="Dynamin_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02212">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00195">
        <property type="entry name" value="DYNAMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00053">
        <property type="entry name" value="DYNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00302">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00410">
        <property type="entry name" value="G_DYNAMIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51718">
        <property type="entry name" value="G_DYNAMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51388">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="8 12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-0505">Motor protein</keyword>
    <keyword evidence="5 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="3" id="KW-0962">Peroxisome biogenesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynamin-type G" evidence="15">
        <location>
            <begin position="43"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="GED" evidence="14">
        <location>
            <begin position="657"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="751"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="772"/>
            <end position="789"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9705626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="831" mass="91431" checksum="4E286050E99F15E1" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E3R1</accession>
    <name>A0AA39E3R1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WAT1-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_005310</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the drug/metabolite transporter (DMT) superfamily. Plant drug/metabolite exporter (P-DME) (TC 2.A.7.4) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E3R1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030184">
        <property type="entry name" value="WAT1-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31218">
        <property type="entry name" value="WAT1-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="139"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="220"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="252"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="282"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="15"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="220"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9703972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="322" mass="35381" checksum="E650E0E5E85810AD" modified="2024-01-24" version="1">MGGKLCNIVDGAKPAIVMVVVQLVFGGVNIFYKLAINDGMNTRVLIAYRYIFATAFISPLAFILERKSGPRLTWMIALQGFLCGLFGGALGQNLYVESIALTSPTFSAAMANLIPAVTFIFAIFLGLEKLAIRTWTGKAKVTSTLLGIGGAMVLTFYKGPQINIWSTKLFLLCNLVDNPGDSDSIFFSNAPKVPSYKFINPISPLECEQAKMGEKYPFDYSSTALMCATASVQTVVYAMCKERNWSAWKLGWNIRLLTVAYTGFLASGLMVVLVSWCVRKRGPLFVSIFNPLMLVFVAILGTLILDEKLHLGRSPLRSPFYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E3X3</accession>
    <name>A0AA39E3X3_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_000567</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E3X3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9708596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="134" mass="14442" checksum="5FF2B4D28C6C1F42" modified="2024-01-24" version="1">MVLPFTPKIRGKGLRFMGNCGLLVAENLEVIPSSTFQSPSSYFPPSCGFTSSFLSPSAPILPSSDIQSLNPLENRVNSEFFFKKDDDGTVGQICVGILNLVLDVNQIAYPNQLTESVNPLMPKTTSPSNLATVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E5A5</accession>
    <name>A0AA39E5A5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WAT1-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_000834</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the drug/metabolite transporter (DMT) superfamily. Plant drug/metabolite exporter (P-DME) (TC 2.A.7.4) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E5A5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030184">
        <property type="entry name" value="WAT1-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31218">
        <property type="entry name" value="WAT1-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="35"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="69"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="156"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="197"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="225"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="251"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="18"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9709043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="304" mass="33421" checksum="36D5E398808B8891" modified="2024-01-24" version="1">MWSLKLTLVMLTIESLDMGLNTISKAAMSRGMSNFVFVVYSNAFAIPFLLICCLLFHRRKPPPPLTTAILCRIFVLGLISCSIQMFMFVGIRYSSPTLASAMTDLVPAFTFILAILTRMENLDLRVRSSRAKSIGTIVSITGALTMTMYKASIIRAYPAELMVTLICCTFVTMQSSIVSLMAERNPSAWRLKPDIELIAIVYSAVLVVGLRSVACTWVMRRKGPFFAAMFKPVGIIIAVVMGVTFLGDTLYLGSVVAAAIIAVGFYTVMWGKAREETLLEDVPAHGMESSSHNVPLLQNKTFQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E5R8</accession>
    <name>A0AA39E5R8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Chorismate mutase</fullName>
            <ecNumber evidence="3 7">5.4.99.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_001082</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>chorismate = prephenate</text>
            <dbReference type="Rhea" id="RHEA:13897"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:29934"/>
            <dbReference type="EC" id="5.4.99.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Metabolic intermediate biosynthesis; prephenate biosynthesis; prephenate from chorismate: step 1/1.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.5" evidence="3 7"/>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E5R8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004106">
        <property type="term" value="F:chorismate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046417">
        <property type="term" value="P:chorismate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901747">
        <property type="term" value="P:prephenate(2-) biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.590.10:FF:000001">
        <property type="entry name" value="Chorismate mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.590.10">
        <property type="entry name" value="Chorismate mutase, AroQ class superfamily, eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036263">
        <property type="entry name" value="Chorismate_II_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008238">
        <property type="entry name" value="Chorismate_mutase_AroQ_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037039">
        <property type="entry name" value="CM_AroQ_sf_eucaryotic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002701">
        <property type="entry name" value="CM_II_prokaryot"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01802">
        <property type="entry name" value="CM_pl-yst"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21145">
        <property type="entry name" value="CHORISMATE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21145:SF15">
        <property type="entry name" value="CHORISMATE MUTASE 3, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01817">
        <property type="entry name" value="CM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017318">
        <property type="entry name" value="Chor_mut_AroQ_eu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48600">
        <property type="entry name" value="Chorismate mutase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51169">
        <property type="entry name" value="CHORISMATE_MUT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5 7" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="6 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chorismate mutase" evidence="8">
        <location>
            <begin position="154"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR017318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9709433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="273" mass="31259" checksum="69AB4DBD88ADA9ED" modified="2024-01-24" version="1">MNSLISREGYVVYPHKERVDASENYTLDSIRHFLIRQEDSIIFSLLERAQYCYNADTYDPHAFSKDGFHGSLVEFIVKETEKLHAQMGRYDSPNEHPFFPAQLPKPMLPPLKYPQVLHPCADSVNLNNKVWNMYFKNLLPRLVKAGDDGNHGSAAVCDTNCLQALSKRIHYGKFVAEAKFLESSDVYEAAIREQDRDKLMALLTYEAVEAAIKKRVEAKAKAFGQEVRSMGEGHDGDDPAYKIKPRLVANLYGDWIMPLTKEVQVEYLLRRLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E629</accession>
    <name>A0AA39E629_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">UBR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_001636</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E629"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15542">
        <property type="entry name" value="PHD_UBR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19677">
        <property type="entry name" value="UBR-box_UBR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000439">
        <property type="entry name" value="Putative E3 ubiquitin-protein ligase UBR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040204">
        <property type="entry name" value="UBR7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047506">
        <property type="entry name" value="UBR7-like_UBR-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003126">
        <property type="entry name" value="Znf_UBR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13513">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UBR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13513:SF9">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UBR7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02207">
        <property type="entry name" value="zf-UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00396">
        <property type="entry name" value="ZnF_UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51157">
        <property type="entry name" value="ZF_UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBR-type" evidence="5">
        <location>
            <begin position="39"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="UBR-type" evidence="4">
        <location>
            <begin position="39"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9706177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="423" mass="47909" checksum="A5304A708B041B72" modified="2024-01-24" version="1">MADVFEEEAEQTVSIQEYLKDVEDQELEADLVLGGDEGKECTYIKGYMKRQAIFSCLTCTMDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGEFFCKLFPNKDIENVENSYNHNFKGSYCTCGRPYPDPDIEEQEEMIQCCICEDWFHEEHLGLESSDEIPRDEEGEPLYEDFICQSCSGVFSFLTLYPKSIWAGVRQHDAPVNNNKEKDVLEGPPSVCGSSQKLENDPYSHNSQQMDHAITNTVSENVPVGKNTFLGENTEKNEGSSQGIQDACPSSSTCVIGVDLLVAPPVFDGSKPMFLSKNWRDILCRCEKCVDFYTQKCINFLLDKEDSIVEYEKMAKQKREEKLHQQEGVELNLLDKLGHVGKMEFLNGVADMKNEIHAFLESFDPSKPITSADVHQVFENLAKKRRRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E6H0</accession>
    <name>A0AA39E6H0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DUF4378 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_001800</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E6H0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022212">
        <property type="entry name" value="DUF3741"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025486">
        <property type="entry name" value="DUF4378"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47212">
        <property type="entry name" value="ADHESIN-LIKE PROTEIN, PUTATIVE (DUF3741)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47212:SF4">
        <property type="entry name" value="ADHESIN-LIKE PROTEIN, PUTATIVE (DUF3741)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12552">
        <property type="entry name" value="DUF3741"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14309">
        <property type="entry name" value="DUF4378"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF3741" evidence="2">
        <location>
            <begin position="146"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4378" evidence="3">
        <location>
            <begin position="637"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="202"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="286"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="500"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="286"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="307"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="512"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9706477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="747" mass="84246" checksum="941228A1FEFB194A" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39EA60</accession>
    <name>A0AA39EA60_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DYW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_000458</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. PCMP-H subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39EA60"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000366">
        <property type="entry name" value="Pentatricopeptide (PPR) repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000031">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001966">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:003114">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032867">
        <property type="entry name" value="DYW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF347">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14432">
        <property type="entry name" value="DYW_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="99"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="200"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="270"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="301"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="DYW" evidence="4">
        <location>
            <begin position="518"/>
            <end position="610"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9708427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="610" mass="68097" checksum="1237FBB22A970852" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YHY2</accession>
    <name>A0AA38YHY2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">TF-B3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_026975</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YHY2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10017">
        <property type="entry name" value="B3_DNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.330.10">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003340">
        <property type="entry name" value="B3_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015300">
        <property type="entry name" value="DNA-bd_pseudobarrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044837">
        <property type="entry name" value="REM16-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31391:SF135">
        <property type="entry name" value="B3 DOMAIN-CONTAINING PROTEIN OS01G0234100-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31391">
        <property type="entry name" value="B3 DOMAIN-CONTAINING PROTEIN OS11G0197600-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02362">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01019">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101936">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50863">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TF-B3" evidence="8">
        <location>
            <begin position="140"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="45"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="293"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="448"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="48"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9670769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="505" mass="56839" checksum="3E8804EAB52BA1A7" modified="2024-01-24" version="1">MGVDQNVVKQEVEDEVFRMASSRESRRSREEDVTLAQLSQTVRTFAPSTPQSLTKTKLKSSTPTKQKKCVEKKEKGFGIKRRRSSFRLKHEASDHSPKSASCKRNKAAVDGSCTPLKAKTSALIRAEEVQSNLGPEFPSFVKVLVRSHVSSCFWMGLPGPFCDMHLPKKDVIITLEDESGESCQIKYIAGKTGLSAGWRRFSMDHKLLEGDVLVFHLVEPTAFKVYIIRANDLTEVDGALGLLELDTHTKNDAGKLVAVSDLDTVACKSSKRKHSKSLPLVVFQKKNRKTGSIPNLMQREQSENDSEGTGSEVLEGSKFFEPVVQFKDISFDNFNILVDGLLIDSELPEDTRAKYYELCCSQNAFLHEKIVEGLNCRLIAEIISQTVQIADTLRACKLTTSRVEFTNWERTLKAFEVLGMNVGFLQARLDHLVSLAFDSEGAMYSRQYMEVTIERAHAEDEMRDLEDKLMELKAASERMDADIETLKEAVENHVIRFQEEVVAPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YJ79</accession>
    <name>A0AA38YJ79_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DOG1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_025238</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YJ79"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051886">
        <property type="entry name" value="Seed_Dev/Stress_Resp_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025422">
        <property type="entry name" value="TGA_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46354:SF12">
        <property type="entry name" value="DNA-BINDING PROTEIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46354">
        <property type="entry name" value="DOG1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14144">
        <property type="entry name" value="DOG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51806">
        <property type="entry name" value="DOG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DOG1" evidence="1">
        <location>
            <begin position="1"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9671433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="231" mass="26627" checksum="F116502A3D13E6A5" modified="2024-01-24" version="1">MSFQSFNEAWFDHLHDLLQQLRLAPKATAPDRDRALLDLVHKVRTHYSQYYRAMSYTARHDVLSLFAAPWSSSLERSLHWVAGWRPAIAFHLIYTHSSILFETHIVDILRGAHNGDLGDLSPAQLQRVSELQCQTVREENEITAELSECFNSVSGLVGAVFDPEENVERLRNVVERAENLRFRTICSVVEILNPQQAVEFLVAVMELQFWIRGMGLCHDRLRENLRGFRGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YJ83</accession>
    <name>A0AA38YJ83_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Bulb-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_025229</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YJ83"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009505">
        <property type="term" value="C:plant-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00028">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.10">
        <property type="entry name" value="Bulb-type lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035446">
        <property type="entry name" value="SLSG/EP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32444">
        <property type="entry name" value="BULB-TYPE LECTIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32444:SF10">
        <property type="entry name" value="CURCULIN-LIKE (MANNOSE-BINDING) LECTIN FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002686">
        <property type="entry name" value="SLG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041216805" description="Bulb-type lectin domain-containing protein" evidence="4">
        <location>
            <begin position="28"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="5">
        <location>
            <begin position="45"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9671421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="444" mass="49553" checksum="55FA595CE72E6C0D" modified="2024-01-24" version="1">MSCSPPSFTLSFIFLSFISISSFTVHASVPLKNTFKYVNEGEFGPYIVEYGGDYRGLPIFASPFQFCFYNTTPNAYTLALRMAIQRTEPIYRWVWEANRGKPVGENATLTFGTDGNLVLAHADGRVAWQTGTANKGVVGLKLLSNGNLVLHDSTGKFIWQSFDYPTDVLLVGQSLRVGGVTKLVSRASAADNSDGKYSLVMEPKRLAMYYKSSNSPTPMLYATSSVWFTIDKGSLQNVTLTGSLDDLGYAYYLNLNYHLSNSPYPTGGAILVRPKYNSTSTFLRLDMDGNIRLYTYFDKVDYQGWQVTYTLFDRDSGGESECQLPERCGKFGLCEDSQCVACPSPKGLMGWSKDCAPLKLSGCGVNDFHYYKLEGVDHFMDKYSDGDGPMKEKQCGDKCSKDCKCLGYFYHTHTSMCWIAYDLKTLSKVQNSTHLAYIKAPNKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YJ96</accession>
    <name>A0AA38YJ96_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORF/ORRM1/DAG-like MORF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_025272</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YJ96"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016554">
        <property type="term" value="P:cytidine to uridine editing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080156">
        <property type="term" value="P:mitochondrial mRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039206">
        <property type="entry name" value="MORF/ORRM1/DAG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054059">
        <property type="entry name" value="MORF/ORRM1/DAG-like_MORF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31346">
        <property type="entry name" value="MULTIPLE ORGANELLAR RNA EDITING FACTOR 2, CHLOROPLASTIC-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31346:SF4">
        <property type="entry name" value="MULTIPLE ORGANELLAR RNA EDITING FACTOR 8, CHLOROPLASTIC_MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21864">
        <property type="entry name" value="MORF_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="MORF/ORRM1/DAG-like MORF" evidence="4">
        <location>
            <begin position="96"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="62"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="211"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="211"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="255"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="291"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="318"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="422"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF21864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9671490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="438" mass="46290" checksum="317CEBCD42286AB8" modified="2024-01-24" version="1">MATLTRCLTRSLPALSSLVSRSTPSLSLSTVSCSTFSLLRLRPLSAAAISVLRHLPQSTSARSFATRQTSSSLNDPNPNWSNRPPKETILLDGCDFEHWLVVMEKPEGEPTRDEIIDSYIKTLAMIVGSEEEARMKIYSVSTRCYFAFGALVSEELSLKIKELPRVRWVLPDSYLDVKNKDYGGEPFIDGKAVPYDPKYHEEWIRNNARANERNRRNDRPRNFDRSRNFERRRENMQNRDFQAPMQNSPPNMGGGPPPNMGGGPPPNMGGGPPPNMGGGPPPNMGMGGGPPSNNFGGPQNNFRGGPPNNYGGAPPSNYGGGAPPSNYGGGAPPSNYGGGAPPSNYGGGAPPSNFGGGAPPSNFGGGAPPSNYGGGAPPSNYGGGAPPSNYGGGAPPNNYGGGMPQSNFGGGTPPNNAGGNASQQQWWNADQQPGRNSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YLY1</accession>
    <name>A0AA38YLY1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Photosystem II 10 kDa polypeptide, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_026228</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Associated with the oxygen-evolving complex of photosystem II.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Chloroplast thylakoid membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the psbR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YLY1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009654">
        <property type="term" value="C:photosystem II oxygen evolving complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015979">
        <property type="term" value="P:photosynthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006814">
        <property type="entry name" value="PSII_PsbR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34369">
        <property type="entry name" value="PHOTOSYSTEM II 10 KDA POLYPEPTIDE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34369:SF7">
        <property type="entry name" value="PHOTOSYSTEM II 10 KDA POLYPEPTIDE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04725">
        <property type="entry name" value="PsbR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="11" id="KW-0604">Photosystem II</keyword>
    <keyword evidence="7" id="KW-0934">Plastid</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0793">Thylakoid</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="113"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9672888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="139" mass="14365" checksum="8355201A92F55EAB" modified="2024-01-24" version="1">MAASVMSSLSLKPSPFSVERRAVKGLPSLARSSSSFRVEASGGKKIKTDKPYGIGGGMKLSEGLDASGRKGKGKGVYQFVDKYGANVDGYSPIYSPEEWSPGGDVYVGGTTGLLIWAVTLAGLLLGGALLVYNTSALAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YNX7</accession>
    <name>A0AA38YNX7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Phosphoribosylaminoimidazole-succinocarboxamide synthase, chloroplastic</fullName>
            <ecNumber evidence="3">6.3.2.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">SAICAR synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_023480</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate + ATP = (2S)-2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamido]succinate + ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:22628"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58443"/>
            <dbReference type="ChEBI" id="CHEBI:77657"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SAICAR synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.6" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YNX7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009570">
        <property type="term" value="C:chloroplast stroma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004639">
        <property type="term" value="F:phosphoribosylaminoimidazolesuccinocarboxamide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01414">
        <property type="entry name" value="SAICAR_synt_Sc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000199">
        <property type="entry name" value="Phosphoribosylaminoimidazole-succinocarboxamide synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000015">
        <property type="entry name" value="Phosphoribosylaminoimidazole-succinocarboxamide synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00137">
        <property type="entry name" value="SAICAR_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028923">
        <property type="entry name" value="SAICAR_synt/ADE2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018236">
        <property type="entry name" value="SAICAR_synthetase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009251">
        <property type="entry name" value="PRK12607.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43700">
        <property type="entry name" value="PHOSPHORIBOSYLAMINOIMIDAZOLE-SUCCINOCARBOXAMIDE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43700:SF1">
        <property type="entry name" value="PHOSPHORIBOSYLAMINOIMIDAZOLE-SUCCINOCARBOXAMIDE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01259">
        <property type="entry name" value="SAICAR_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01057">
        <property type="entry name" value="SAICAR_SYNTHETASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01058">
        <property type="entry name" value="SAICAR_SYNTHETASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAICAR synthetase/ADE2 N-terminal" evidence="11">
        <location>
            <begin position="89"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00073447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9673953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="384" mass="43003" checksum="9BBF6174FDC6DDC4" modified="2024-01-24" version="1">MAQCIPGLNPPKTLSGNVFSSISYCKTGPRLIKFLTMSSQQHQSQQLSLDALISSNRKEEVVGAVRHSLSNCLSETNLHLTVPALKSKTRGKVRDVYDGGDYLVLVTTDRQSAFDRVLASIPFKGQVLNETSLWWFDKTQHIAPNAVVSAPDKNVTIAKKCSVFPVEFVVRGYVTGSTDTSLWTVYKNGVRNYCGNALPDGMVKNQKLPANILTPTTKAADHDVPIAPDEIVKLGLMTQADYDEVSRKALSLFEYGQHVALEHGLILVDTKYEFGKGSDGIVLLIDEVHTPDSSRYWIANSYEECFRNGIEPENVDKEFLRLWFKEHCNPYEDEVLPDAPAELVSELAWRNLYMIESPGMFPWPWHLCNKCAKVFVVVSLLKFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YQL0</accession>
    <name>A0AA38YQL0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">50S ribosomal protein L31</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_023973</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL31 family. Type A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YQL0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.30">
        <property type="entry name" value="Ribosomal protein L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034704">
        <property type="entry name" value="Ribosomal_bL28/bL31-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002150">
        <property type="entry name" value="Ribosomal_bL31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042105">
        <property type="entry name" value="Ribosomal_bL31_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00105">
        <property type="entry name" value="L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001809">
        <property type="entry name" value="PRK00528.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33280">
        <property type="entry name" value="50S RIBOSOMAL PROTEIN L31, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33280:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL31C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01197">
        <property type="entry name" value="Ribosomal_L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01249">
        <property type="entry name" value="RIBOSOMALL31"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143800">
        <property type="entry name" value="L28p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9674762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="135" mass="15252" checksum="2BE931A2956953F3" modified="2024-01-24" version="1">MALTLSNTFLQRKLSPPTLPIKKANAVRACRPQWTCRKKEIHPQFYEDAKVYCNGELVMTTGGTQKEYVVDVWSGNHPFYLGNRSGLLVDADQVEKFRKKFGEISQIMEIPVLKYGEIVLPPKRKPGSGKGGKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YVE0</accession>
    <name>A0AA38YVE0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Strictosidine synthase conserved region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_022242</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the strictosidine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YVE0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018119">
        <property type="entry name" value="Strictosidine_synth_cons-reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10426:SF136">
        <property type="entry name" value="PROTEIN STRICTOSIDINE SYNTHASE-LIKE 9-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10426">
        <property type="entry name" value="STRICTOSIDINE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03088">
        <property type="entry name" value="Str_synth"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63829">
        <property type="entry name" value="Calcium-dependent phosphotriesterase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041398694" description="Strictosidine synthase conserved region domain-containing protein" evidence="5">
        <location>
            <begin position="19"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="domain" description="Strictosidine synthase conserved region" evidence="6">
        <location>
            <begin position="138"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="Strictosidine synthase conserved region" evidence="6">
        <location>
            <begin position="393"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="domain" description="Strictosidine synthase conserved region" evidence="6">
        <location>
            <begin position="555"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="domain" description="Strictosidine synthase conserved region" evidence="6">
        <location>
            <begin position="847"/>
            <end position="939"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9677144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1031" mass="111989" checksum="24AD4C3FFD4C922D" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YVR3</accession>
    <name>A0AA38YVR3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HNH nuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_022427</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YVR3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00085">
        <property type="entry name" value="HNHc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002711">
        <property type="entry name" value="HNH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003615">
        <property type="entry name" value="HNH_nuc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33427">
        <property type="entry name" value="HNH ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33427:SF3">
        <property type="entry name" value="HNH ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01844">
        <property type="entry name" value="HNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00507">
        <property type="entry name" value="HNHc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HNH nuclease" evidence="2">
        <location>
            <begin position="102"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9677432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="198" mass="22295" checksum="6CEF4B9557B02D4A" modified="2024-01-24" version="1">MRPIKERRRSRSSPARDRPIGPSGFEPRPSGSRSTRLSPSSTSLLDTPFTTEEEDATLFEKLKISPNPNFNPRSFPYSVKQQCWEKAEKIKGRDPERWRRDALGNIVFRKLVGCPGCLCHDYDHIVPYSKGGKSTLENCQVLQATVNRSKGNRTEISKADLIQKSSYCRVSGRDMDLLELSAYGNVRRGQDSGGCTIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YW85</accession>
    <name>A0AA38YW85_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_022685</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YW85"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF78">
        <property type="entry name" value="OS12G0587133 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="114"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9677853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="835" mass="95369" checksum="2183CC665E952DCC" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YXE1</accession>
    <name>A0AA38YXE1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">O-fucosyltransferase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_020608</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycan metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase GT106 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YXE1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006004">
        <property type="term" value="P:fucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024709">
        <property type="entry name" value="FucosylTrfase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019378">
        <property type="entry name" value="GDP-Fuc_O-FucTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31741">
        <property type="entry name" value="OS02G0726500 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31741:SF69">
        <property type="entry name" value="RHAMNOGALACTURONAN I RHAMNOSYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10250">
        <property type="entry name" value="O-FucT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009360">
        <property type="entry name" value="UCP009360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="11" id="KW-0294">Fucose metabolism</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9678466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="320" mass="36864" checksum="80827B6A0837114D" modified="2024-01-24" version="1">MVAIARFLNVTLIIPKIKPPFGLIQVRILKELPPRLKTKVELGMFYSLPPVSWSNISYHLHQILPLVQKHKVVNMNKTDAHLANNGLPLEIQKLLCRVNFSSLRLTSQIEELGRRVVRILRENGPFLVLHLSYEMDMLAFSSCIRSCNDKEAEELARMRYAYPWWKEKIIDSKVKSREGLCPLTLGETALILSALGIIMFKFILLLDKYMVEKEEWPVQKETVLKPSDLRFFQNHISQMAALDYLVSLESDIFVPTYDGNMAKGVEGHCRFLGFKKTIQLDRRLLVNLADENKNGGAPTTFRKMLNMENYHRMSISLGVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YXZ3</accession>
    <name>A0AA38YXZ3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_020661</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YXZ3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000041">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000213">
        <property type="entry name" value="Tyrosine-sulfated glycopeptide receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046956">
        <property type="entry name" value="RLP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063">
        <property type="entry name" value="LRR RECEPTOR-LIKE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063:SF101">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE FLS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="889"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="13">
        <location>
            <begin position="20"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9678538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1029" mass="113910" checksum="517012046546EBFF" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YY98</accession>
    <name>A0AA38YY98_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SMC hinge domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_020976</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YY98"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051276">
        <property type="term" value="P:chromosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1060.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1480.30">
        <property type="entry name" value="Designed four-helix bundle protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010935">
        <property type="entry name" value="SMC_hinge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036277">
        <property type="entry name" value="SMC_hinge_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43977">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06470">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00968">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75553">
        <property type="entry name" value="Smc hinge domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SMC hinge" evidence="3">
        <location>
            <begin position="363"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="108"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="169"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="564"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="736"/>
            <end position="780"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9678931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="964" mass="109338" checksum="994916AA00C7D5BF" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YYM2</accession>
    <name>A0AA38YYM2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Clp ATPase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_021145</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YYM2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000002">
        <property type="entry name" value="ATP-dependent Clp protease ATP-binding subunit ClpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050052">
        <property type="entry name" value="ATP-dep_Clp_protease_ClpX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019489">
        <property type="entry name" value="Clp_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48102:SF7">
        <property type="entry name" value="ATP-DEPENDENT CLP PROTEASE ATP-BINDING SUBUNIT CLPX-LIKE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48102">
        <property type="entry name" value="ATP-DEPENDENT CLP PROTEASE ATP-BINDING SUBUNIT CLPX-LIKE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07724">
        <property type="entry name" value="AAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10431">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01086">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Clp ATPase C-terminal" evidence="3">
        <location>
            <begin position="105"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9679125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="229" mass="24656" checksum="021D0B9BE34FA404" modified="2024-01-24" version="1">MSATVVSLSSQAGYVGEDVESILHKLLMVAEFNVEAAQQGLVYIDEVDKITKKAKSLNISRDVSGEGVQQALLKMLEGTVSASIVESGDLIAYGLIPEFTGRFPISVLTEPENALGKQYKKMLSMNNVKLHFTENALRLVAKKAIAKNPGARSLRGILEKILTEAMFEIPDVKTGTDMVDAVLVDEEAVESIEAQGCGAKVLRGEGALEQFLHETKSSNPLACFPCAPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YZL0</accession>
    <name>A0AA38YZL0_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_021521</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YZL0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9679621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="110" mass="12157" checksum="84253CE2E724B989" modified="2024-01-24" version="1">MHPDHSSRPHLTRLLGAMSKPSTAVCIRKLTAVLLRECILYNEFNGNGRWSLPKPMLSTIVMSNGARTAVLRGRSYLVKGVAWDLIGSLIVSQTDNETVVIKAMVHSFHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z191</accession>
    <name>A0AA38Z191_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ripening-related protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_019610</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the kiwellin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z191"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd22270">
        <property type="entry name" value="DPBB_kiwellin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039271">
        <property type="entry name" value="Kiwellin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33191:SF58">
        <property type="entry name" value="RIPENING-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33191">
        <property type="entry name" value="RIPENING-RELATED PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24300">
        <property type="entry name" value="KWL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041340642" description="Ripening-related protein 2" evidence="5">
        <location>
            <begin position="27"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9680342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="188" mass="20392" checksum="DF2D7D7F2CF7C477" modified="2024-01-24" version="1">MKTFGLKASLLLMAILFFIDFLNVEGQVCHPSGKIRGKKPPPGECNQGNDSDCCKEGKLYTTYKCSPTVSSHTKAYLTLNGFEKDEDGGAPSECDNQYHSDDTPVVALSTGWYSQGSRCLNNITITANGRSVVAMVVDECDSTMGCDSDHDYQPPCANNIVDASRAVWKALGVPHDQWGGLDITWTDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z3U2</accession>
    <name>A0AA38Z3U2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Homeobox protein knotted-1-like 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_017908</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TALE/KNOX homeobox family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9681755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z3U2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005539">
        <property type="entry name" value="ELK_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005540">
        <property type="entry name" value="KNOX1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005541">
        <property type="entry name" value="KNOX2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050224">
        <property type="entry name" value="TALE_homeobox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11850">
        <property type="entry name" value="HOMEOBOX PROTEIN TRANSCRIPTION FACTORS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03789">
        <property type="entry name" value="ELK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03790">
        <property type="entry name" value="KNOX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03791">
        <property type="entry name" value="KNOX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01188">
        <property type="entry name" value="ELK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01255">
        <property type="entry name" value="KNOX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01256">
        <property type="entry name" value="KNOX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51213">
        <property type="entry name" value="ELK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 5" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ELK" evidence="9">
        <location>
            <begin position="194"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="8">
        <location>
            <begin position="214"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox; TALE-type" evidence="5">
        <location>
            <begin position="215"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="161"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="168"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9681755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="310" mass="34900" checksum="C84243B6E6BE93B5" modified="2024-01-24" version="1">MDDLYRLQHEGISISGANAAAATIDFRRSLENSNVYRFESVETEAGMSDLIKSQIVNHPRYPNLVSAYIECRKVGAPPEMASLLEEIGRESQPMNSRSGEIGADPELDEFMESYCEVLHRYKEELSKPFDEATSFLSDIESQLSNLCKGALTAGTSGSYYSDEAAGTSEEELSCGEAEVSESQESLGARPGDQELKEMLMRKYSGYLSSLRKEFLKKRKKGKLPKDARTMLLEWWNTHYRWPYPTEDEKLKLSEVTGLDQKQINNWFINQRKRHWKPSEDMRFALIDGVGGNMGGGPVFLDTRGGTGSNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z3W2</accession>
    <name>A0AA38Z3W2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">U1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_017926</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9681785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z3W2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000491">
        <property type="entry name" value="zinc finger matrin-type protein 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040107">
        <property type="entry name" value="Snu23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45986">
        <property type="entry name" value="ZINC FINGER MATRIN-TYPE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45986:SF1">
        <property type="entry name" value="ZINC FINGER MATRIN-TYPE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="U1-type" evidence="6">
        <location>
            <begin position="83"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="22"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="143"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="22"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="150"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="165"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9681785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="204" mass="23883" checksum="36AF9479551D34E8" modified="2024-01-24" version="1">MAQSSNNAVGVDNTFRRKFDKEEYLERARKREQEEEEGGRHKSKSKGPPVQRKPLKHRDYEVDLESRLGKTQVVTPIAPLSQQAGYYCSVCECVVKDSANYLDHINGKKHQRALGMSMRVERASLQQVQERFEVLKKRRTPGSFTEQDLDERILKQQQEEEERKRQRRERKKEKKKEKALEEEPELDPDVATMMGFGGFRSSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z4C9</accession>
    <name>A0AA38Z4C9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">40S ribosomal protein S21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_017978</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS21 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9681867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z4C9"/>
    <dbReference type="SMR" id="A0AA38Z4C9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1230.20:FF:000002">
        <property type="entry name" value="40S ribosomal protein S21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1230.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001931">
        <property type="entry name" value="Ribosomal_eS21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018279">
        <property type="entry name" value="Ribosomal_eS21_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038579">
        <property type="entry name" value="Ribosomal_eS21_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10442">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01249">
        <property type="entry name" value="Ribosomal_S21e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002148">
        <property type="entry name" value="Ribosomal_S21e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00996">
        <property type="entry name" value="RIBOSOMAL_S21E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR002148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9681867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="82" mass="9233" checksum="345156994EF6C4E1" modified="2024-01-24" version="1">MQNEEGQNMDLYIPRKCSATNRLITSKDHASVQINIGHLDESGTYTGQFTTFALCGFVRAQGDADSALDRLWQKKKAEVRQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z556</accession>
    <name>A0AA38Z556_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Mon2/Sec7/BIG1-like dimerisation and cyclophilin-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_018492</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z556"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032629">
        <property type="entry name" value="DCB_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16213">
        <property type="entry name" value="DCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mon2/Sec7/BIG1-like dimerisation and cyclophilin-binding" evidence="1">
        <location>
            <begin position="10"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9682578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="162" mass="17481" checksum="CFE5848ADF5A6F35" modified="2024-01-24" version="1">MVCSCVDNLSSHIHGKPLLGVIRICYIIALNSKSPINQATSKAMLTQMISIIFRRMETDPVCTSSGSAANKEATLADNLNYEVETSSGDQTEKEMTLGDALSMNQVKDIALASVEELQNLAGGVDIKGLEAVLDKAVHLEDGKKMTRGINMCYLSKSSKSRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z587</accession>
    <name>A0AA38Z587_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_018528</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z587"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9682623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="130" mass="14484" checksum="723D8B8977E5F31E" modified="2024-01-24" version="1">MRHNIDVMHVEKNVYDNVVNTLLNIVGKTKDTEKALLGSRSGYIKGLGHGPRPPSSSSKSTHKSHREIELENELKATRELLQSQETRIQHQQSQIAQLASFVSEMSQHMHIPGSSSRSSSPLDNTPLMDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z6B8</accession>
    <name>A0AA38Z6B8_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_018870</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z6B8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08588">
        <property type="entry name" value="PI-PLCc_At5g67130_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051057">
        <property type="entry name" value="PI-PLC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593:SF51">
        <property type="entry name" value="F21F23.12 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26178">
        <property type="entry name" value="PI-PLC_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041341444" evidence="1">
        <location>
            <begin position="22"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9683060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="393" mass="43597" checksum="402C7B7761A1F43C" modified="2024-01-24" version="1">MGLGANLFFILLSLFFSVATACTDGKCRLLDECSTDEDCGAGLYCFSCHEGYSRSRCVRSSITDQFKVLNNSLPFNKYAFLTTHNSYAIDGEQSHTGVPRLTFTNQEDSVTQQLNNGARGLMLDTYDFKGDVWLCHSIGGECHDYTAFGPAIDTLREVEAFLSANPVEIVTLILEDYVKAPNGLTKVFTDAGLMKYWFPVTSMPQNGEDWPLVSDMVAKNQRLIVFTSIKSKQESEGIAYQWNYMVENQYGGGGMYDLGNCLARGESSPLNDEAKSLVLVNYFNTIPLKQPICEQNSGGLINMLQTCYEAAGNRWANFVAVDYYKRSEGGGSFQAADTMNAKLLCGCHDIHACAALSTYGACTPSEAITNITHYERVQALMKFVWKMNHIRVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z7D5</accession>
    <name>A0AA38Z7D5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">WIYLD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_016328</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9683781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z7D5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.850">
        <property type="entry name" value="Histone-lysine N methyltransferase , C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043017">
        <property type="entry name" value="WIYLD_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018848">
        <property type="entry name" value="WIYLD_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34271">
        <property type="entry name" value="NUCLEOLAR HISTONE METHYLTRANSFERASE-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34271:SF1">
        <property type="entry name" value="NUCLEOLAR HISTONE METHYLTRANSFERASE-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10440">
        <property type="entry name" value="WIYLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WIYLD" evidence="2">
        <location>
            <begin position="8"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="132"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9683781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="202" mass="22740" checksum="A6D661ED57A410AF" modified="2024-01-24" version="1">MPPRRRAKKVGLKRMDAAVENMRLLGFSDELVRSTIKRLLEVYGDAGWLFIEEGSYKLLIDTILDEQENGLFKGDSSKNNATDDNGLSSTAMPSTGVEDATSWTNEIGSEDCNPPAEEERTNERDISLDQNPLHSPPHLISSSAPIDSLLMQRRRRFCGWISDNEPNDICTKQLIPATVMQLEKLIHGTDTHSKSRWDEKPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z9L9</accession>
    <name>A0AA38Z9L9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Retrovirus-related Pol polyprotein from transposon 17.6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_017128</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z9L9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.370:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 17.6-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041373">
        <property type="entry name" value="RT_RNaseH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17917">
        <property type="entry name" value="RT_RNaseH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="310"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="8">
        <location>
            <begin position="67"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase RNase H-like" evidence="9">
        <location>
            <begin position="248"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase zinc-binding" evidence="10">
        <location>
            <begin position="450"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF17917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9684976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="504" mass="58416" checksum="6F0CEB021BAC8D93" modified="2024-01-24" version="1">MEEEAKPIRQPQRRLNPHLQEVVRAEVLKLLQAGIIYPISDSPWVSPTQVVPKKSGITVIQNESGEEIATRLTSGWRVCIDYRKLNVVTKKDHFPLPFIDQVLERVSGHPFYCFLDGYSGYFQIEIDVEDQEKTTFTCPFGTYAYRRMPFGLCNAPVTFQRCMLSIFSDMVERIMEVFMDDITVYGGTFEECLVNLEAVLNRCIEKDLVLNWEKCHFMFIWDEKCQKSFEQLKQFLTTAPIVRAPNWQLPFEVMCDASDFAIGAVLGQKEDGKPYVIYYASKTLNEAQRNYTTTEKELLAVVFALDKFRAYWVGSFIIVFTYHSALKYLLTKQDAKARLIRWILLLQEFDLQIRDKKGVENVVADHLSRLAIAHNSHVLLINDDFPEESLMLLEKAPWYAHIANYLVTGEVPSEWKAQDRKHFFAKIHAYYWEEPFLFKYCADQIIRKCVPEQEQQGILSHCHENACGGHFASQKTAMKVLQSGFTCPSLFKDSHIMCRSCDRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z9Y9</accession>
    <name>A0AA38Z9Y9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NAD-dependent epimerase/dehydratase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_017252</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z9Y9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08958">
        <property type="entry name" value="FR_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000085">
        <property type="entry name" value="Dihydroflavonol reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF575">
        <property type="entry name" value="NAD-DEPENDENT EPIMERASE_DEHYDRATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="4">
        <location>
            <begin position="9"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9685146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="313" mass="34410" checksum="BCF776DE5841088C" modified="2024-01-24" version="1">MSGEGKVVSVIGASGFIASWLVKLLLQHHDPKKTQHLLSLDGAQERLHLFKADLLEEGSFDSVVDGCDGVFHSASPVALEAINPQAELIDPALKGTINVLRSCSKVPSVKRVVVTSSLASVFLTGKPLTPEVLIDESWFSDPALCKESKQWYVLSKTLAEEAAWKFSKENGIDMVTINPGWVIGPLLQPTLNLSAEQVLNLINGAQTFPNISSWWVDVRDVANAHIQAYEIPEASGRYCLVERDLHNSEILKILRKLYPGLPLPEKCADDKPYAPSSQVSQEKAKSLVIHFTPLEVSLKDTVESLKEKNFVSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZAR1</accession>
    <name>A0AA38ZAR1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Polygalacturonase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_017447</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZAR1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051801">
        <property type="entry name" value="GH28_Enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31339:SF12">
        <property type="entry name" value="ENDO-POLYGALACTURONASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31339">
        <property type="entry name" value="PECTIN LYASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9685412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="138" mass="14974" checksum="3F5C29A00731AAD5" modified="2024-01-24" version="1">MSQAQEEKNNKGNEFKNFPTKAQPLQRVVLEVVSGILVLGLLMSVRVAECGRASSVEYLAMNCWKHSAVLKIFGGNGDGKKMKTKAFKSAIDHLSERASDGGAELIVPPGKWLTGSFNLTSHFTLYVHKDAVILGGQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZBD8</accession>
    <name>A0AA38ZBD8_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_017714</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZBD8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37748">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37748:SF1">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="14"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9685772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="58" mass="6162" checksum="6746BD4A686E7B63" modified="2024-01-24" version="1">MVFSAILSCFVPSSSSSRVFNDAGGSSVKEPKSKSSKSSSRAPIPVSYFPVNSYLSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZC51</accession>
    <name>A0AA38ZC51_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Dihydroorotase, mitochondrial</fullName>
            <ecNumber evidence="3">3.5.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_015277</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24296"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30864"/>
            <dbReference type="ChEBI" id="CHEBI:32814"/>
            <dbReference type="EC" id="3.5.2.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the metallo-dependent hydrolases superfamily. DHOase family. Class II DHOase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.2.3" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZC51"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004151">
        <property type="term" value="F:dihydroorotase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006221">
        <property type="term" value="P:pyrimidine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01294">
        <property type="entry name" value="DHOase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000006">
        <property type="entry name" value="Dihydroorotase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00219">
        <property type="entry name" value="PyrC_classII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004721">
        <property type="entry name" value="DHOdimr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002195">
        <property type="entry name" value="Dihydroorotase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00856">
        <property type="entry name" value="pyrC_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43137">
        <property type="entry name" value="DIHYDROOROTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43137:SF1">
        <property type="entry name" value="DIHYDROOROTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001237">
        <property type="entry name" value="DHOdimr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00482">
        <property type="entry name" value="DIHYDROOROTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00483">
        <property type="entry name" value="DIHYDROOROTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="10">
        <location>
            <begin position="41"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00069884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9686301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="377" mass="41624" checksum="D41F713C6F822054" modified="2024-01-24" version="1">MSSRHPDMPCASILPFARIEVPNQVKVKGARMELSLTQPDDWHLHLRDGDLLEAVVSHSASHFGRGIVMPNLKPPITTTAAAVAYRESILKALPANCDFTPLMTLYLTDKTSPNEIKLARNSGVVFAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQNMPLLVHGEVTNPEVDVFDREKVFIDTVLIPLIQKLPRLKVVMEHITTMDAVRFVESCNEGSVAATVTPQHLVLNRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGSRKFFLGTDSAPHERRRKECPCGCAGIYNAPVALSLYAKVFEEAGALDKLEAFTSFNGPDFYGLPRNTSKIKLSKTPWKVPEYYSFSFGDIVPMFAGETLDWLPSSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZD85</accession>
    <name>A0AA38ZD85_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phytocyanin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_015548</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZD85"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04216">
        <property type="entry name" value="Phytocyanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000003">
        <property type="entry name" value="Blue copper"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039391">
        <property type="entry name" value="Phytocyanin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003245">
        <property type="entry name" value="Phytocyanin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33021">
        <property type="entry name" value="BLUE COPPER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33021:SF492">
        <property type="entry name" value="UCLACYANIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02298">
        <property type="entry name" value="Cu_bind_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51485">
        <property type="entry name" value="PHYTOCYANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0186">Copper</keyword>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041421019" description="Phytocyanin domain-containing protein" evidence="5">
        <location>
            <begin position="21"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="Phytocyanin" evidence="6">
        <location>
            <begin position="21"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="119"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="126"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="167"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="181"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="206"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9686749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="243" mass="24616" checksum="B0955D5C9D2F781D" modified="2024-01-24" version="1">MLRSQMSLAVTALLIQLGMAANYTVGGPNGGWDTSSNLQAWASAQTFIVGDNLIFQFTPNHDVLEVSKADYNTCSTSNPTQTYSSSPAVIPLSSPGKRCFICGMAGHCSQGMKIELDTLASSSPPSASPSSPPTSSPASPVTPPTSSPSSPVTPPTSSPASPVTAPTSPPPLEMTPPPKSTPTISPASLSTTPTSSPAPSSDVPSAEPPTSSPSPSPSSAHKGSFEASLIMGFGLMMTILLAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZEG7</accession>
    <name>A0AA38ZEG7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_015735</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000440">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025724">
        <property type="entry name" value="GAG-pre-integrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054722">
        <property type="entry name" value="PolX-like_BBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057670">
        <property type="entry name" value="SH3_retrovirus"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439">
        <property type="entry name" value="GAG-POL-RELATED RETROTRANSPOSON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439:SF484">
        <property type="entry name" value="REVERSE TRANSCRIPTASE TY1_COPIA-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13976">
        <property type="entry name" value="gag_pre-integrs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22936">
        <property type="entry name" value="Pol_BBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25597">
        <property type="entry name" value="SH3_retrovirus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="3">
        <location>
            <begin position="484"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="209"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="254"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="210"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="267"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9686998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1343" mass="149339" checksum="CA31027C1C2FDFF8" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZGC3</accession>
    <name>A0AA38ZGC3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_014054</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZGC3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11393">
        <property type="entry name" value="bHLH_AtbHLH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044658">
        <property type="entry name" value="bHLH92/bHLH041-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045239">
        <property type="entry name" value="bHLH95_bHLH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055477">
        <property type="entry name" value="DUF7049"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055478">
        <property type="entry name" value="DUF7050"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46665:SF1">
        <property type="entry name" value="SPERMATOGENESIS- AND OOGENESIS-SPECIFIC BASIC HELIX-LOOP-HELIX-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46665">
        <property type="entry name" value="TRANSCRIPTION FACTOR BHLH041-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23132">
        <property type="entry name" value="DUF7049"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23133">
        <property type="entry name" value="DUF7050"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="8">
        <location>
            <begin position="341"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="169"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="387"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="184"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9688122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="522" mass="58736" checksum="96632C7D353DBAD0" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZHE7</accession>
    <name>A0AA38ZHE7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DUF659 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_014498</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZHE7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007021">
        <property type="entry name" value="DUF659"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008906">
        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32166:SF74">
        <property type="entry name" value="OS05G0256350 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32166">
        <property type="entry name" value="OSJNBA0013A04.12 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04937">
        <property type="entry name" value="DUF659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF659" evidence="2">
        <location>
            <begin position="150"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="HAT C-terminal dimerisation" evidence="3">
        <location>
            <begin position="512"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="676"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="682"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="703"/>
            <end position="716"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9688880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="716" mass="82558" checksum="E2B77B94DE836F95" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZHH7</accession>
    <name>A0AA38ZHH7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Histone H2A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_014707</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9689181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZHH7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002119">
        <property type="entry name" value="Histone_H2A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23430">
        <property type="entry name" value="HISTONE H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00414">
        <property type="entry name" value="H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9689181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="107" mass="11911" checksum="C3B004901A58782B" modified="2024-01-24" version="1">MEVVERVKKGARGRNGGGPKKKPISHSVKVGLQFPVGHISKYLKKGRHSQRIGTVAEVLELAGNAVGDNKKNRALLAVRNDEELRNHHQRDEVEIIIGMVMEDVREV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZI58</accession>
    <name>A0AA38ZI58_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">PVL29_014953</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="25">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="25">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9689520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZI58"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000383">
        <property type="entry name" value="Leucine-rich repeat receptor protein kinase EMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000101">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000358">
        <property type="entry name" value="Putative leucine-rich repeat receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000661">
        <property type="entry name" value="Serine-threonine protein kinase plant-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45974:SF29">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45974">
        <property type="entry name" value="RECEPTOR-LIKE PROTEIN 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 22" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="13 22" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="11 23" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="23">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041376445" description="non-specific serine/threonine protein kinase" evidence="23">
        <location>
            <begin position="23"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="24">
        <location>
            <begin position="647"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="675"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="KAJ9689520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="928" mass="102496" checksum="669412E8DF72C62C" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZK06</accession>
    <name>A0AA38ZK06_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Oligopeptide transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_012605</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oligopeptide OPT transporter (TC 2.A.67.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZK06"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035673">
        <property type="term" value="F:oligopeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004648">
        <property type="entry name" value="Oligpept_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004813">
        <property type="entry name" value="OPT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00727">
        <property type="entry name" value="ISP4_OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00728">
        <property type="entry name" value="OPT_sfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22601">
        <property type="entry name" value="ISP4 LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03169">
        <property type="entry name" value="OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0571">Peptide transport</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="32"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="122"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="199"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="269"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="331"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="360"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="443"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="490"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="519"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="564"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="593"/>
            <end position="614"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9690027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="653" mass="72850" checksum="A774CB18CBC4EB30" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZKF6</accession>
    <name>A0AA38ZKF6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">cysteine--tRNA ligase</fullName>
            <ecNumber evidence="5">6.1.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Cysteinyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PVL29_012881</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Plastid</location>
            <location evidence="3">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.16" evidence="5"/>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZKF6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004817">
        <property type="term" value="F:cysteine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006423">
        <property type="term" value="P:cysteinyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00672">
        <property type="entry name" value="CysRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000009">
        <property type="entry name" value="Cysteine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1910:FF:000003">
        <property type="entry name" value="Cysteine--tRNA ligase CPS1, chloroplastic/mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1910">
        <property type="entry name" value="Cysteine-tRNA ligase, C-terminal anti-codon recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00041">
        <property type="entry name" value="Cys_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015803">
        <property type="entry name" value="Cys-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024909">
        <property type="entry name" value="Cys-tRNA/MSH_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032678">
        <property type="entry name" value="tRNA-synt_1_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00435">
        <property type="entry name" value="cysS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10890:SF26">
        <property type="entry name" value="CYSTEINE--TRNA LIGASE 1, CYTOPLASMIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10890">
        <property type="entry name" value="CYSTEINYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01406">
        <property type="entry name" value="tRNA-synt_1e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00983">
        <property type="entry name" value="TRNASYNTHCYS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="7" id="KW-0436">Ligase</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0934">Plastid</keyword>
    <keyword evidence="13" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="tRNA synthetases class I catalytic" evidence="18">
        <location>
            <begin position="21"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00031499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF01406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ9690455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="516" mass="58371" checksum="FDC474C948188967" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZLJ0</accession>
    <name>A0AA38ZLJ0_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_013381</name>
        <name evidence="5" type="ORF">PVL29_013385</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZLJ0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001865">
        <property type="entry name" value="Ribosomal_uS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005707">
        <property type="entry name" value="Ribosomal_uS2_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023591">
        <property type="entry name" value="Ribosomal_uS2_flav_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11489">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN SA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00395">
        <property type="entry name" value="RIBOSOMALS2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52313">
        <property type="entry name" value="Ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9691183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9691187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="111" mass="12326" checksum="F0382CF25E064B27" modified="2024-01-24" version="1">MPTVLQFEKLHLCSLWAVLFQDGSLNGDDIAIENPKDPIVQSDRIHGQRAVLKFAQYIGVHAITGECIPRALTNRPQISFNDLRLLILTDPRTDHQPVKEAALGNAPTIAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZM07</accession>
    <name>A0AA38ZM07_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Disease resistance R13L4/SHOC-2-like LRR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_013678</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZM07"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050905">
        <property type="entry name" value="Plant_NBS-LRR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463:SF198">
        <property type="entry name" value="RPP4C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="4">
        <location>
            <begin position="267"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9691566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="442" mass="50666" checksum="E341619264A94F4C" modified="2024-01-24" version="1">MRTQKEFHLQHLGEDEAWNLFKKTAGDTVKKRELRPIAVDVAKKCEGLPVAIVTIANALRDERVGVWENALEELRRSAPTNIRGVRKNVYSCVELSYDHLEGDEVKSLFLLCALLGDGDISMDRLLQYAMGLNLFKGIYSWDKASNKLITLVENLKASSLLLDGEYEDDEKSLFFYESMLLRMHDVVRDVARSIASKDIHRFVVKEAVGLQEEWQWMDGCRNCTRISLKCKIIYELPRGLVCPELEFLLLNSSNYLKIPDAFFQDTKQLRVLDLSEVSLSPPPSSLGFLTNLRTLCLNLNRGELKDIAVIGELKKLQVFSLVDSVIRQLPKKMMQLSDLRVLDLQHCGWLEVIPRNVISSLSRLEYLSMKESFHIEWEAEGFNSRKRINACLSELKHLSSLRTLEIVVSDPSLLPEDDMLFDNLNLTRYTIVIGNRMVYDGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZN47</accession>
    <name>A0AA38ZN47_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Jacalin-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_011292</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the jacalin lectin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZN47"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09612">
        <property type="entry name" value="Jacalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.100.10.30:FF:000001">
        <property type="entry name" value="Jacalin-related lectin 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.100.10.30">
        <property type="entry name" value="Jacalin-like lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001229">
        <property type="entry name" value="Jacalin-like_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033734">
        <property type="entry name" value="Jacalin-like_lectin_dom_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036404">
        <property type="entry name" value="Jacalin-like_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47293:SF15">
        <property type="entry name" value="JACALIN-RELATED LECTIN 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47293">
        <property type="entry name" value="JACALIN-RELATED LECTIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01419">
        <property type="entry name" value="Jacalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00915">
        <property type="entry name" value="Jacalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51101">
        <property type="entry name" value="Mannose-binding lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51752">
        <property type="entry name" value="JACALIN_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0430">Lectin</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Jacalin-type lectin" evidence="3">
        <location>
            <begin position="39"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9692146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="197" mass="21499" checksum="6E1DB0568EB519C8" modified="2024-01-24" version="1">MLIETFHPPGDLYQHHSPPHLPCSSSHLSNWWINMEGGKMLSVVVGPWGGGGSSWDDGSYSGVREITICYGWCIDSIRVVYDKNGEPVSGDKHGGSGGQSSEIKLQYPEEKLIGVSGHYHPFWFCGPVIRSLTFNSNRRTFGPFGAQVGTPFSLTTDGGQIVGFQGRSGWYLDAIGFRWSGTDPSTKLLSPMVCTIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZQS8</accession>
    <name>A0AA38ZQS8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Prolamin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_009492</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9693561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZQS8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008502">
        <property type="entry name" value="Prolamin-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05617">
        <property type="entry name" value="Prolamin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041379023" description="Prolamin-like domain-containing protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="Prolamin-like" evidence="3">
        <location>
            <begin position="53"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9693561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="129" mass="14941" checksum="4FF0985134CE193A" modified="2024-01-24" version="1">MARLTYVIPTMVAFFLCSAMAVDLRNLNDKTVPWYAQVIPPEPFQGFYGKIIQCMWKLDPICGKQVYTRIFNPSTSMNDYCCRNIATVGVTCNRYLGWALSLFEKFKRYAGLIDTRSKIIFIDCIKRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZR14</accession>
    <name>A0AA38ZR14_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Intron-binding protein aquarius</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_009558</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CWF11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9693661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17935">
        <property type="entry name" value="EEXXQc_AQR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000507">
        <property type="entry name" value="Pre-mRNA-splicing factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003210">
        <property type="entry name" value="RNA helicase aquarius"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048966">
        <property type="entry name" value="Aquarius_b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048967">
        <property type="entry name" value="Aquarius_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032174">
        <property type="entry name" value="Aquarius_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026300">
        <property type="entry name" value="CWF11_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045055">
        <property type="entry name" value="DNA2/NAM7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887:SF5">
        <property type="entry name" value="RNA HELICASE AQUARIUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16399">
        <property type="entry name" value="Aquarius_N_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21143">
        <property type="entry name" value="Aquarius_N_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21144">
        <property type="entry name" value="Aquarius_N_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038901">
        <property type="entry name" value="AQR_cwf11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="1" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNA helicase aquarius N-terminal" evidence="5">
        <location>
            <begin position="51"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="domain" description="RNA helicase aquarius beta-barrel" evidence="6">
        <location>
            <begin position="547"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="domain" description="RNA helicase aquarius insertion" evidence="7">
        <location>
            <begin position="767"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase helicase" evidence="3">
        <location>
            <begin position="1022"/>
            <end position="1113"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase-like C-terminal" evidence="4">
        <location>
            <begin position="1122"/>
            <end position="1314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1406"/>
            <end position="1491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1407"/>
            <end position="1420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1421"/>
            <end position="1433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1452"/>
            <end position="1463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1464"/>
            <end position="1473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR038901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF16399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF21143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF21144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9693661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1491" mass="171460" checksum="FD49C5047904E247" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZR55</accession>
    <name>A0AA38ZR55_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cytochrome P450 82C4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_011815</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZR55"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20654">
        <property type="entry name" value="CYP82"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000026">
        <property type="entry name" value="Cytochrome P450 82C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050651">
        <property type="entry name" value="Plant_Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47947">
        <property type="entry name" value="CYTOCHROME P450 82C3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47947:SF1">
        <property type="entry name" value="CYTOCHROME P450 82E3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11 12" id="KW-0349">Heme</keyword>
    <keyword evidence="8 11 12" id="KW-0408">Iron</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 12" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="11">
        <location>
            <position position="458"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9692893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="518" mass="57609" checksum="3314840ED9F8EE90" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZSF9</accession>
    <name>A0AA38ZSF9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_009877</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZSF9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47447">
        <property type="entry name" value="OS03G0856100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47447:SF28">
        <property type="entry name" value="PENTACOTRIPEPTIDE-REPEAT REGION OF PRORP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="181"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="218"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="253"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="288"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="323"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="358"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9694100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="468" mass="53550" checksum="1120906C8D1311B6" modified="2024-01-24" version="1">MQTLKNTIANQSPSPQSPINSHFLSILIDSFRIYNSDPTPNAYRFVISTLTRSRQFHHLPPLLHRLEKVEKFETPEFIFINLIKVYGNANMFEEAVDLFFRIPNFRCVPSVYSLNSLLYVLCKRREGLVMVPQILLKSQAMNIRLEESSFRILVAALCRIKKHNYAIRILNYMLNDGYAVDAKMCSFILSSLCEQKGLSGDEVLRFMEEMRKLGFYPGRVDCNNVIRFLVKEGMMMDALGVFDQMKTDGIKPDTVSYTMILKGVTADGDYEKADDLFDEMLVLGVVPDIHAYNVYINSLCKQNNIEEGVRMLASMRELGCKPDYVTYNMLLEGMSKVRDLGGMRELAREMELEGVQWNWETYRIMLDGLVGKGEIDESCSLLEEMLDKYFSCWCSTFDEIICELCQRGLVCKALQLVNKMVSKTIAPGARAWEALLLGSVEFSFAETSLTELVNPIQIHPVRLPEHQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZSM7</accession>
    <name>A0AA38ZSM7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_009981</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZSM7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000024">
        <property type="entry name" value="Somatic embryogenesis receptor kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47988">
        <property type="entry name" value="SOMATIC EMBRYOGENESIS RECEPTOR KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041387020" description="Leucine-rich repeat-containing N-terminal plant-type domain-containing protein" evidence="4">
        <location>
            <begin position="28"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="5">
        <location>
            <begin position="28"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="6">
        <location>
            <begin position="76"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9694264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="215" mass="23536" checksum="5247D19616FD1D69" modified="2024-01-24" version="1">MGRGFGSMAALVALTVLSVMRVGLVRGNSEGDALYTLRRSLSDPDNVLQSWDPNLVNPCTWFHITCNQDGRVTRVDLGSSNLSGHLVPELGKLEHLQYLELYKNNIQGTIPVELGNLKNLISLDLYNNNISGVIPPALGKLKSLVFLRLNDNQLTGPIPRELVGISTLKVVDVSNNNLCGTIPTTGPFEHILLNKLHDIRASGLGLIIIRHYLLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZSR3</accession>
    <name>A0AA38ZSR3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein DETOXIFICATION</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Multidrug and toxic compound extrusion protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_009567</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9693673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZSR3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13132">
        <property type="entry name" value="MATE_eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045069">
        <property type="entry name" value="MATE_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="113"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="143"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="179"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="206"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="251"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="284"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="328"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="372"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="401"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="431"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9693673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="485" mass="53558" checksum="AEFC9787349668BD" modified="2024-01-24" version="1">MDNNMEERLLSSEADSTSNLKGRIWDESKKMWRVAFPAILSRVTSYGMLVVTQSFVGHISQLDLSGYALTQNVIIRFVNGIVLGMSSATETLCGQAFGAKQYHMMGIYLQRSWIVDLVVATILSPIFIFATPLFKLLGQEDDIAIAAGNISLWFLPILYYFVFSMTIQMYLQAQLKNMIIGWLSGSSIVLHVLLSWIFVIKLNLGIPGAMGALIISGWSMIIGQFIYIFGGWCPQTWRGFSKAAFSDILPVVKLSISSGFMICLELWYYAIILLLAGYLKNASVAISAFSICLNINAWEFMIPFGFLSGSCVRVSNELGRGNAKAAKFSIKVVLSTSICIGVFFWLLCLVFGHDIAYLFTSDEEVVEMVSSLSVLLSFSILLNSVQPVLTGVAVGGGWQAVVAIVNVGCYYVIGIPLGVLLAYVADLSVRGMWIGMLCGVGAQTLVLMYMTWRTDWNDQVKKASDRLNRWLLQSPEESNQSTLQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZTJ6</accession>
    <name>A0AA38ZTJ6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Heat shock protein 70</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_010250</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZTJ6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd10233">
        <property type="entry name" value="ASKHA_NBD_HSP70_HSPA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.10:FF:000028">
        <property type="entry name" value="Heat shock 70 kDa protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.30:FF:000019">
        <property type="entry name" value="Heat shock 70 kDa protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000172">
        <property type="entry name" value="Heat shock 70 kDa protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000465">
        <property type="entry name" value="Heat shock cognate 70 kDa protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000026">
        <property type="entry name" value="Heat shock protein 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001413">
        <property type="entry name" value="PRK00290.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0346">Stress response</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="619"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="619"/>
            <end position="628"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9694685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="649" mass="71083" checksum="9D01016DFF21E526" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZTM9</accession>
    <name>A0AA38ZTM9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Kinesin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_010530</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Responsible for microtubule translocation. May be important for the organization of phragmoplast-specific arrays of microtubules. Plays an essential role in stabilizing the mitotic spindle. Required during mitotic cytokinesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. KIN-5/BimC subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZTM9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005876">
        <property type="term" value="C:spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008574">
        <property type="term" value="F:plus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090307">
        <property type="term" value="P:mitotic spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051231">
        <property type="term" value="P:spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01364">
        <property type="entry name" value="KISc_BimC_Eg5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000019">
        <property type="entry name" value="Kinesin-like protein KIN-5D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047149">
        <property type="entry name" value="KIF11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047241">
        <property type="entry name" value="KIF11-like_kin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47970:SF12">
        <property type="entry name" value="KINESIN FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47970">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6 10" id="KW-0505">Motor protein</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="12">
        <location>
            <begin position="12"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="419"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="98"/>
            <end position="105"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9695076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1009" mass="113460" checksum="57434F9E9B71A32C" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZUR9</accession>
    <name>A0AA38ZUR9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dirigent protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_010869</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Secreted</location>
            <location evidence="4">Extracellular space</location>
            <location evidence="4">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the plant dirigent protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZUR9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009699">
        <property type="term" value="P:phenylpropanoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.480.10">
        <property type="entry name" value="Allene oxide cyclase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044859">
        <property type="entry name" value="Allene_oxi_cyc_Dirigent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004265">
        <property type="entry name" value="Dirigent"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21495">
        <property type="entry name" value="NUCLEOPORIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03018">
        <property type="entry name" value="Dirigent"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0052">Apoplast</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041488861" description="Dirigent protein" evidence="4">
        <location>
            <begin position="20"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9695597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="188" mass="20471" checksum="697335DC0410FFE9" modified="2024-01-24" version="1">MAKTLTYFAFLFFSTVAAAVGDGHTFSRDLSPESLDLKREKLSHLHFYFHDAITGPNPTVMRVAEAAVTNKSATLFGGVAVLDDPLTVGPEPSSKLVGRAQGMYAAASQEELGLLMVMNLAFMEGKYNGSTLSVLGRNTIFSKVREMPIVGGSGVFRYARGYVEARTYSFNVISGNAVVEYNVYAFHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZX67</accession>
    <name>A0AA38ZX67_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_008953</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZX67"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF78">
        <property type="entry name" value="OS12G0587133 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase zinc-binding" evidence="1">
        <location>
            <begin position="377"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9696975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="561" mass="64337" checksum="475EB42FFA2536DB" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZX79</accession>
    <name>A0AA38ZX79_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_008900</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZX79"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16461">
        <property type="entry name" value="RING-H2_EL5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044600">
        <property type="entry name" value="ATL1/ATL16-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46913">
        <property type="entry name" value="RING-H2 FINGER PROTEIN ATL16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46913:SF8">
        <property type="entry name" value="RING-TYPE E3 UBIQUITIN TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="37"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="12">
        <location>
            <begin position="128"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="217"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="238"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9696901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="351" mass="39882" checksum="9A21D2E61B32BD57" modified="2024-01-24" version="1">MDRFHMHFSNHGSEALVSIKTHENPIYQPSSPASDTAFPILAIAVLSIMATAFLLVSYYIFVIKCCLSWHHIELLRRFSTPQSRQQEDPLMDYSPTFLNRGLDESLIHQIPTFLFRRGQSEEGSFHGCVVCLNEFQEHEMIRVLPNCSHAFHLDCIDIWLQSNANCPLCRSSISGTTRYRNDPIIAPSSSPQDPRPFSEALMGGDDDFVVIELGGGDDRGVTLPPRQQERADSRELLVQSTGPSPTKLQQKLENKKSRKFHYVSSMGDECIDVREKDDQFSIQPIRRSFSMDSAADPQLYMTVQEIIRHKNRPLSEVSTSQECDSRVRRSFFSFGHGRGSRNAVLPIEFLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZXA3</accession>
    <name>A0AA38ZXA3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_008888</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZXA3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052426">
        <property type="entry name" value="Plant_dev_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45801">
        <property type="entry name" value="OS07G0101800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45801:SF117">
        <property type="entry name" value="OS07G0417400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="33"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="149"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="53"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9696887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="175" mass="19668" checksum="ED6F45571B9ECE8E" modified="2024-01-24" version="1">MESGPREEARNSSEETERPEQVDDEDMITGPSYDCVFCKRGFTTAQALGGHMNIHRKDRAKAKPPSSVLPKKPEENFVNSGFCVSILSYKPPNSTAPEAQINYQEYILASTSPARDPIDDFRAEKQETPNVCKEDWRTCLSLQVGPSCAYDSKEKGQDEKQEDELDLELRLGHNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A0A9</accession>
    <name>A0AA39A0A9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Isovaleryl-CoA dehydrogenase, mitochondrial</fullName>
            <ecNumber evidence="5">1.3.8.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PVL29_007320</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>3-methylbutanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = 3-methylbut-2-enoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:12276"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57344"/>
            <dbReference type="ChEBI" id="CHEBI:57345"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="EC" id="1.3.8.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 15 16">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid degradation; L-leucine degradation; (S)-3-hydroxy-3-methylglutaryl-CoA from 3-isovaleryl-CoA: step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 16">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.8.4" evidence="5"/>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A0A9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008470">
        <property type="term" value="F:3-methylbutanoyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006552">
        <property type="term" value="P:L-leucine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01156">
        <property type="entry name" value="IVD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.540.10:FF:000007">
        <property type="entry name" value="Isovaleryl-CoA dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000003">
        <property type="entry name" value="isovaleryl-CoA dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000004">
        <property type="entry name" value="Isovaleryl-CoA dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034183">
        <property type="entry name" value="IVD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884:SF12">
        <property type="entry name" value="ISOVALERYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016578">
        <property type="entry name" value="HsaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00072">
        <property type="entry name" value="ACYL_COA_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00073">
        <property type="entry name" value="ACYL_COA_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15 16" id="KW-0274">FAD</keyword>
    <keyword evidence="7 16" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10 16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="19">
        <location>
            <begin position="29"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="18">
        <location>
            <begin position="146"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="17">
        <location>
            <begin position="253"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="266"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="147"/>
            <end position="156"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="180"/>
            <end position="182"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="202"/>
            <end position="203"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="264"/>
            <end position="267"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="360"/>
            <end position="364"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="387"/>
            <end position="388"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="389"/>
            <end position="391"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00052875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR634183-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR634183-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR634183-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAJ9698180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="405" mass="44594" checksum="F00AF21CE4032323" modified="2024-01-24" version="1">MMQRVFSARSLFRKDRLRAAFSTALLFDDTQIQFKESIAQFAQENIAPHASRIDRTNYFPEEVNLWKLMGDFNLHGITAPEEYGGLGLGYLYHCIAMEEISRASGSVALSYGAHSNLCINQLVRNGNPAQKQKYLPKLISGEHVGALAMSEPNAGSDVVSMKCKADRVDGGYILNGNKMWCTNGPIAQTLVVYAKTDITAHSKGITAFIIEKGMPGFSTAQKLDKLGMRGSDTCELVFENCFVPEENVLGQEGKGVYVMMSGLDLERLVLAAGPLGLMQACIDAVLPYVRQREQFGRPIGEFQFIQGKLADMYTSLQSSRSYVYSVARNCENGKIDPKDCAGVILCAAERATQVALQAIQCLGGNGYVNEYPTGRLLRDAKLYEIGAGTSEIRRMIIGRELFKEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A3H3</accession>
    <name>A0AA39A3H3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptide-methionine (R)-S-oxide reductase</fullName>
            <ecNumber evidence="5">1.8.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_005815</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the reduction of methionine sulfoxide (MetSO) to methionine in proteins. Plays a protective role against oxidative stress by restoring activity to proteins that have been inactivated by methionine oxidation. MSRB family specifically reduces the MetSO R-enantiomer.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-methionyl-[protein] + [thioredoxin]-disulfide + H2O = L-methionyl-(R)-S-oxide-[protein] + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:24164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="Rhea" id="RHEA-COMP:12313"/>
            <dbReference type="Rhea" id="RHEA-COMP:12314"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16044"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:45764"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.8.4.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="5">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the MsrB Met sulfoxide reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.8.4.12" evidence="5"/>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A3H3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033743">
        <property type="term" value="F:peptide-methionine (R)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030091">
        <property type="term" value="P:protein repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.20">
        <property type="entry name" value="Peptide methionine sulfoxide reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028427">
        <property type="entry name" value="Met_Sox_Rdtase_MsrB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002579">
        <property type="entry name" value="Met_Sox_Rdtase_MsrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011057">
        <property type="entry name" value="Mss4-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00357">
        <property type="entry name" value="peptide-methionine (R)-S-oxide reductase MsrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46081">
        <property type="entry name" value="PEPTIDE METHIONINE SULFOXIDE REDUCTASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46081:SF11">
        <property type="entry name" value="PEPTIDE METHIONINE SULFOXIDE REDUCTASE B4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01641">
        <property type="entry name" value="SelR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51316">
        <property type="entry name" value="Mss4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51790">
        <property type="entry name" value="MSRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MsrB" evidence="6">
        <location>
            <begin position="11"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU365044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9700171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="138" mass="14918" checksum="A17B7FB0CFCB4604" modified="2024-01-24" version="1">MAAEGSIQKSEEEWRAILSPEQYQILRQKATEPRGTGEYDKFYGDGIYNCAGCGTPLYKSTTKFNSGCGWPAFYEGLPGAINRTPDPDGRRTEITCAACGGHLGHVFKGEGFSTPTDERHCVNSVSIKFAPPNSSASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A4K8</accession>
    <name>A0AA39A4K8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Beta-amylase</fullName>
            <ecNumber evidence="3 10">3.2.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_006272</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>Hydrolysis of (1-&gt;4)-alpha-D-glucosidic linkages in polysaccharides so as to remove successive maltose units from the non-reducing ends of the chains.</text>
            <dbReference type="EC" id="3.2.1.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the glycosyl hydrolase 14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.2" evidence="3 10"/>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A4K8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016161">
        <property type="term" value="F:beta-amylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001554">
        <property type="entry name" value="Glyco_hydro_14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018238">
        <property type="entry name" value="Glyco_hydro_14_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001371">
        <property type="entry name" value="Glyco_hydro_14B_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31352">
        <property type="entry name" value="BETA-AMYLASE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31352:SF31">
        <property type="entry name" value="BETA-AMYLASE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01373">
        <property type="entry name" value="Glyco_hydro_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00750">
        <property type="entry name" value="BETAAMYLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00842">
        <property type="entry name" value="GLHYDLASE14B"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00506">
        <property type="entry name" value="BETA_AMYLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00679">
        <property type="entry name" value="BETA_AMYLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6 10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 10" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="281"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="479"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="394"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="399"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="441"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="480"/>
            <end position="481"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="515"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601554-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR601554-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9700863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="573" mass="63229" checksum="CEBE1C51E7356B8B" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A4X6</accession>
    <name>A0AA39A4X6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">KIB1-4 beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_006237</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A4X6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050942">
        <property type="entry name" value="F-box_BR-signaling"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005174">
        <property type="entry name" value="KIB1-4_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44259:SF15">
        <property type="entry name" value="F-BOX PROTEIN KIB2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44259">
        <property type="entry name" value="OS07G0183000 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03478">
        <property type="entry name" value="Beta-prop_KIB1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KIB1-4 beta-propeller" evidence="1">
        <location>
            <begin position="59"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9700819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="374" mass="42426" checksum="B35D0A1F0B3D7331" modified="2024-01-24" version="1">MALKGEFRPCFQTPWLMLPSSRVEEGEEDGEGEGGISFFDLAEKIEYGDVCMCGEVHVYEGCCVGSSHGWLVLLDETANLSVFDPFSGDQIQVGSLKEGLEFTDVDAYTVMKDPRAFVPKAIVMSDGKKNKDKDGVVLIYGSRKKLAFCTYRDGKFMDLDGAHQPYHDIIYFDNVVCALSDTGSVEVWDFSGSFPTKITDMTPSFPLKTANFGRSFKDLYSSHFYLVESCGELLFVVRFIGEFVDGDGKPVREADLLGDEDIQPKICPYKTLLFHVYRLDRYKIKWVEMESLGDQVVFLGGNHSMSLRVGDCPGCVKDSIYFTDSYWERVDEDYSYGGHDMGYYNLRDRSVKPFYDTNRCKMKPTPFWVVSNPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A5A7</accession>
    <name>A0AA39A5A7_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_006549</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endomembrane system</location>
            <topology evidence="7">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A5A7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01868">
        <property type="entry name" value="Rab11_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000274">
        <property type="entry name" value="ras-related protein RABA5a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0636">Prenylation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9701250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="286" mass="31847" checksum="8E6D3DCA1FBA8218" modified="2024-01-24" version="1">MTLKALPFIMNPSVTLPTTAAPSTASNYYCRASFTPLLYCFSFFSPLESLLHVSDLFLINREKTKVTEFSIMEDEEGEEYLFKVVIIGDSAVGKSNLLSRYARNEFNINSKATIGVEFQTQSMEIDGKEVKAQIWDTAGQERFRAVTSAYYRGAVGALVVYDISRRSTFDSVARWLDELKTHSETTVARMLVGNKSDLENIRDVSVEEGKSLAEAEGLFFIETSALDSTNVKKAFEIVIREIYNNISRKVLNSDSYKAELSVNRVSLVGNGADGAKQANSRYSCCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A5Q4</accession>
    <name>A0AA39A5Q4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Polygalacturonase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_006705</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the glycosyl hydrolase 28 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A5Q4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004650">
        <property type="term" value="F:polygalacturonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000016">
        <property type="entry name" value="Polygalacturonase 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000743">
        <property type="entry name" value="Glyco_hydro_28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31375">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00295">
        <property type="entry name" value="Glyco_hydro_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00502">
        <property type="entry name" value="POLYGALACTURONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0134">Cell wall</keyword>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6 9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041238125" description="Polygalacturonase" evidence="10">
        <location>
            <begin position="26"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9701466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="393" mass="41287" checksum="5F0AB719839AC611" modified="2024-01-24" version="1">MVQYVSSSSLLLAAVLFMVLQPYWATAVTYNVVNLGAKGDGHTDSTKAFLNAWAAACGSASPATIYVPPGRYLLRNAVFRGCKNHAITFRIDATLVAPSDYRVIGNAANWLAFQDVTGVSIIGGVLDGQGTGLWACKRSGKSCPNGATTLGLTKSNNILIRGLTSLNSQMFHIVINGCHIVKLQGVRVSASGHSPNTDGIHVQLSSGVTILDTQIKTGDDCVSIGPGATNLWIENVACGPGHGISIGSLGKDLEEEGVQNVTVKTVTFTGSQNGVRIKSWARASNGFVKGVVFQHILMVNVQNPIVIDQNYCPGHKNCPGQVSGVKVSDVTYQDIHGTSASEVAVKFDCSSKNPCSGIKLQDVKLTYRNKAPESSCVNAGGMASGFVEPASCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A687</accession>
    <name>A0AA39A687_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">chitinase</fullName>
            <ecNumber evidence="2">3.2.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PVL29_006866</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1-&gt;4)-beta-linkages in chitin and chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.14"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.14" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A687"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008843">
        <property type="term" value="F:endochitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016998">
        <property type="term" value="P:cell wall macromolecule catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050832">
        <property type="term" value="P:defense response to fungus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031640">
        <property type="term" value="P:killing of cells of another organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00325">
        <property type="entry name" value="chitinase_GH19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00035">
        <property type="entry name" value="ChtBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.10:FF:000006">
        <property type="entry name" value="Agglutinin isolectin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.20.10:FF:000001">
        <property type="entry name" value="Endochitinase (Chitinase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.530.10:FF:000052">
        <property type="entry name" value="Endochitinase PR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.530.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.20.10">
        <property type="entry name" value="Endochitinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.10">
        <property type="entry name" value="Endochitinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001002">
        <property type="entry name" value="Chitin-bd_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018371">
        <property type="entry name" value="Chitin-binding_1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036861">
        <property type="entry name" value="Endochitinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016283">
        <property type="entry name" value="Glyco_hydro_19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000726">
        <property type="entry name" value="Glyco_hydro_19_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22595:SF194">
        <property type="entry name" value="CHITINASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22595">
        <property type="entry name" value="CHITINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00187">
        <property type="entry name" value="Chitin_bind_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00182">
        <property type="entry name" value="Glyco_hydro_19"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001060">
        <property type="entry name" value="Endochitinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00451">
        <property type="entry name" value="CHITINBINDNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00270">
        <property type="entry name" value="ChtBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="Lysozyme-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57016">
        <property type="entry name" value="Plant lectins/antimicrobial peptides"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00026">
        <property type="entry name" value="CHIT_BIND_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50941">
        <property type="entry name" value="CHIT_BIND_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00773">
        <property type="entry name" value="CHITINASE_19_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00774">
        <property type="entry name" value="CHITINASE_19_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0929">Antimicrobial</keyword>
    <keyword evidence="11" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="9" id="KW-0146">Chitin degradation</keyword>
    <keyword evidence="5 16" id="KW-0147">Chitin-binding</keyword>
    <keyword evidence="10 15 16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0295">Fungicide</keyword>
    <keyword evidence="12" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0611">Plant defense</keyword>
    <keyword evidence="13" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 17" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041245057" description="chitinase" evidence="17">
        <location>
            <begin position="25"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-1" evidence="18">
        <location>
            <begin position="24"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="14">
        <location>
            <position position="133"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15 16">
        <location>
            <begin position="29"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15 16">
        <location>
            <begin position="34"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="89"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="151"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="238"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR001060-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR001060-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ9701672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="270" mass="28406" checksum="BDF9A98795D72445" modified="2024-01-24" version="1">MAVKLLTILLVGVLLAALQGSTVAQNCGCPSGLCCSKFGYCGTGNAYCGEGCQAGPCNSSGGSSGGVSVADVVTQAFFDGIINQTAGNCEGKPFYTRAAFLSALNSYPGFGKGGSANQSKREIAAFFAHVTHETGHFCYINEMGGASKNYCNSSYTQYPCVAGQKYYGRGPLQLTWNYNYGPAGRSIGFNSLSNPGIVATNPDISFKTALWFWMNSVHSVINQGFGATIRAINGAAECNGRKPSEVNARVQYYKNYTSQFGVSPGDNVTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A882</accession>
    <name>A0AA39A882_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_004448</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A882"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35834:SF3">
        <property type="entry name" value="ARM REPEAT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35834">
        <property type="entry name" value="ARMADILLO-TYPE FOLD PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9702722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="381" mass="42297" checksum="DCDC6DF0605DB313" modified="2024-01-24" version="1">MGEASNKHNQHVFTVLEAIKKASKDLQTNPISFTNTCQSTIKSLLELKNEANSFLSSDPSLLKLSQLLNSLKALLDKLENSQGYGLRSFVRRQLTYYEASRVASSIIDEIKAWVDHESLKNLVKTIEESGDEDEKIEVLKKFESRVSQGFDRDLQDLILRTKIFTIVESILCDSQCSVSIREEAAAVIVSLVRFNKDVFVGLVLMGPTIRVLISISSCRSIQVLTSLIKLIKSPLIDIIESNGEIPKIISLLSSPDLSIQVVAMDCILEIGYFGRKEAIEAMFGDGLIEKLMELQRSENEGDSITETENGGGGCEEPEMGLKRRFSSCVARFAVQLEVGEGLRQREKIEFKLEVLKRVKESSVSEAEAATIVAEVLWGSSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ACB9</accession>
    <name>A0AA39ACB9_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_002819</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ACB9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000695">
        <property type="entry name" value="MYB transcription factor MYB48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000001">
        <property type="entry name" value="MYB-related transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015495">
        <property type="entry name" value="Myb_TF_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47994">
        <property type="entry name" value="F14D16.11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47994:SF5">
        <property type="entry name" value="F14D16.11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="HTH myb-type" evidence="6">
        <location>
            <begin position="9"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="5">
        <location>
            <begin position="9"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="6">
        <location>
            <begin position="62"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="5">
        <location>
            <begin position="62"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9704427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="251" mass="27764" checksum="DDD91CE87466DD34" modified="2024-01-24" version="1">MGRSPCCEKAHTNKGAWTKEEDDRLIAYIRAHGEGCWRSLPKAAGLLRCGKSCRLRWINYLRPDLKRGNFTEEEDELIIKLHSLLGNKWSLIAGRLPGRTDNEIKNYWNTHIRRKLLNRGIDPSTHRPINEPSPDVTTISFAATVKEEEKINISSTGGFGCKTEKNPVTEKCPDLNLELRISPPYQPQAEAPLKTGGRSSSTTLCFACSLGIPDSEECSCSIGTSSGSSSSGYDFLGLTSGVLDYRGLEMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ACH7</accession>
    <name>A0AA39ACH7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Alpha/beta hydrolase fold-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_003174</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDXG' lipolytic enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ACH7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050466">
        <property type="entry name" value="Carboxylest/Gibb_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033140">
        <property type="entry name" value="Lipase_GDXG_put_SER_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23024">
        <property type="entry name" value="ARYLACETAMIDE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23024:SF467">
        <property type="entry name" value="CARBOXYLESTERASE 12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01174">
        <property type="entry name" value="LIPASE_GDXG_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="3">
        <location>
            <begin position="76"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9705002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="322" mass="35827" checksum="436197D211598805" modified="2024-01-24" version="1">MDSSDNSELTFNFPPFLRVFKDGRVERFMGTDTVPPSLNVENGVHSKDIVIEPETGISAKLYIPKITYPSQKLPLLIYFHGGGFCIETSSSPTYHNYLDSLVAEGNVVAVSVNYRRAPEDPLPVAYDDCWTAVKWVVSHSNSQGLEPWLNDHANFNHLFLAGDSAGANLAHNMAIRAGTRVNELGSVKVSGIILVHPYFWGKDPIGSEMNDLQKKARVDSLWHFVCPTTSGCDDPLINPATDPQLGSLGCEKVLIFLAEKDMLRDRGWFYYETLGKSGWDGAVDLTEAEAEDHVFHIFKPTCEKAVAMLKRMALFLNPQNFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AD12</accession>
    <name>A0AA39AD12_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">SWI/SNF complex subunit SWI3D</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_003049</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AD12"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02336">
        <property type="entry name" value="ZZ_RSC8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000014">
        <property type="entry name" value="SWI/SNF complex subunit SMARCC2 isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041984">
        <property type="entry name" value="Rsc8/Ssr1/Ssr2_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032451">
        <property type="entry name" value="SMARCC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007526">
        <property type="entry name" value="SWIRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000433">
        <property type="entry name" value="Znf_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043145">
        <property type="entry name" value="Znf_ZZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12802:SF41">
        <property type="entry name" value="BRAHMA ASSOCIATED PROTEIN 155 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12802">
        <property type="entry name" value="SWI/SNF COMPLEX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04433">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16495">
        <property type="entry name" value="SWIRM-assoc_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00569">
        <property type="entry name" value="ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00291">
        <property type="entry name" value="ZnF_ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50934">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01357">
        <property type="entry name" value="ZF_ZZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50135">
        <property type="entry name" value="ZF_ZZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIRM" evidence="14">
        <location>
            <begin position="161"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="ZZ-type" evidence="13">
        <location>
            <begin position="321"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="12">
        <location>
            <begin position="375"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="16">
        <location>
            <begin position="375"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="15">
        <location>
            <begin position="378"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="433"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="606"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="940"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="9"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="26"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="41"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="53"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="114"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="438"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="459"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="511"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="627"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="693"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="719"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="735"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="778"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="806"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="831"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="970"/>
            <end position="987"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="988"/>
            <end position="998"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9704817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="19">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1015" mass="110091" checksum="42C119BE32D5DF72" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AE05</accession>
    <name>A0AA39AE05_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_003793</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
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            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
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            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
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        </lineage>
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    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001268">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein, mitochondrial isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF460">
        <property type="entry name" value="OS01G0815900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="155"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="186"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9705861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="305" mass="34503" checksum="6D161E589AF4B90A" modified="2024-01-24" version="1">MKLSLTIKLSAHLQQRNLLSLLLKHQTRRSAMQQLHSHLITTGAHYHCMDVTSWNTLLRHYSLGIFPQEAFLLFRHLRLHHVSPPLSFNSFSFSFLLKACAYLQHRKGGLQLHALTLKAGFEFHVYVHTVLLNVYAVCGALLEAKQVFDEMPVRNSVTWNVLITGLAKWGELHLARSLFDEMPMPTDVSWTTIIGGYTRMNQPKQALALFCTKFIDEGIKPTEITLLAIFPAISNLGALEICQLVHTYGEKRGLNASDIRIRNSLLDTYAKCGCIESASRKLGLMDISNLCICNAWNGERSHSKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AEW2</accession>
    <name>A0AA39AEW2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_001675</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AEW2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16461">
        <property type="entry name" value="RING-H2_EL5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044600">
        <property type="entry name" value="ATL1/ATL16-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46913:SF23">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RHA4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46913">
        <property type="entry name" value="RING-H2 FINGER PROTEIN ATL16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="12">
        <location>
            <begin position="102"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="154"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="165"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="193"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="206"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9706262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="247" mass="27551" checksum="6620A1B8F7E0B0DF" modified="2024-01-24" version="1">MGLPQTPTPHLYPEALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASTPSSTAPILPSSMNQAASFLSPSCEVGLKGELKGKLPTILFDEELKARDSLCCVCLGEFEIKEELHQVPSCKHVFHADCIYHWLRTNSTCPLCRCSVFPNTKAGNPVSPVRPDQPDNQNSNQHRQNVSSQQQEENIGVRLDISDSSSGQQMVPTDGSSSSSLREPGRSSELPTCMDSEMGYQDQESIVLHIETHSET</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AID0</accession>
    <name>A0AA39AID0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Fiber protein Fb34</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_000308</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the DESIGUAL family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AID0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009606">
        <property type="entry name" value="DEAL/Modifying_wall_lignin1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052222">
        <property type="entry name" value="DESIGUAL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31769">
        <property type="entry name" value="OS07G0462200 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06749">
        <property type="entry name" value="DUF1218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="95"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="142"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9708183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="190" mass="20560" checksum="63872E18420E14B2" modified="2024-01-24" version="1">MGEGKGSTLVHLLVVVLSLVAFGFAVAAERRRSVGTIVTDDRNATYCVYNSDVATGYGVGGFLFLLTSESLLMGVTKCMCFGRPLAPGGNRAWSIIYFVSSWMTFLVAEACLIAGATKNAYHTKYRGMIYAQNFSCETLRKGVFIAGAVFVVATMILNVYYYMYFSKATDTQAVRKANRASSTVGMAGYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AKR0</accession>
    <name>A0AA39AKR0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">O-fucosyltransferase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_000671</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycan metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase GT106 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AKR0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006004">
        <property type="term" value="P:fucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11299">
        <property type="entry name" value="O-FucT_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024709">
        <property type="entry name" value="FucosylTrfase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019378">
        <property type="entry name" value="GDP-Fuc_O-FucTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052272">
        <property type="entry name" value="GT106_glycosyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31933">
        <property type="entry name" value="O-FUCOSYLTRANSFERASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31933:SF1">
        <property type="entry name" value="PROTEIN PECTIC ARABINOGALACTAN SYNTHESIS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10250">
        <property type="entry name" value="O-FucT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009360">
        <property type="entry name" value="UCP009360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="10" id="KW-0294">Fucose metabolism</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9708770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="383" mass="44307" checksum="30301FA4F129C933" modified="2024-01-24" version="1">MQICNAVAVAKIMNATLILPLLKQDQIWKDQTKFEDIFDVDHFIDYLKDDVRIVRDIPEWFTDKSELLTSIRRTVKNIPKYAPAQFYIDNVLPRIKEKKIMALKPFVDRLGYDNVPPEINRLRCRVNYHALKFLPEIEQMADQLASRMRNRTGSSNPYMALHLRFEKGMVGLSFCDFVGTREEKAQMAEYRKKEWPRRYKNGSHLWQLALQKRKEGRCPLEPGEVAVILRAMGYPKETQIYVASGKVYGGQNRMAPLRNMFPNLVTKEELATKEELDGFRKHVTSLAALDFLVCLKSDVFVMTHGGNFAKLIIGARRYMGHRLKSIKPDKGLMSKSFGDPYMGWATFVEDVVVTHQTRTGLPEETFPNYDLWENPLTPCMCKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AKU9</accession>
    <name>A0AA39AKU9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_000343</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AKU9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000590">
        <property type="entry name" value="PR5-like receptor kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000178">
        <property type="entry name" value="serine/threonine-protein kinase PBS1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045874">
        <property type="entry name" value="LRK10/LRL21-25-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27009">
        <property type="entry name" value="RUST RESISTANCE KINASE LR10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041289723" description="Protein kinase domain-containing protein" evidence="14">
        <location>
            <begin position="35"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="268"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="333"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="361"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9708238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="638" mass="71910" checksum="A07986078E4042CF" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AM09</accession>
    <name>A0AA39AM09_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_000603</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AM09"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044550">
        <property type="term" value="P:secondary metabolite biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000014">
        <property type="entry name" value="NAD(P)-linked oxidoreductase superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11732">
        <property type="entry name" value="ALDO/KETO REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00063">
        <property type="entry name" value="ALDOKETO_REDUCTASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="4">
        <location>
            <begin position="45"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="3">
        <location>
            <position position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9708659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="317" mass="35701" checksum="B6504CD6D1BAFB20" modified="2024-01-24" version="1">MRSIPEMALGSTGKDIPLVGMGTAVYPFAPSEAMIDSILTAIELEKPLGETIKKAVELGLIKSRNELFITSKLWWSDAHHDRVLPALQNTLKNLQLDYLDLYLIHWPVSLKPGKYELPVNKDDLLPIDLGSVWKAMEDCQKLGLTKAIGVNNFSCKKLEELLQTASILPAVNQVGSERGLFVKVEMNPLWQQKKLREFCAEKGIHITAYSPLGAKGTLWGTDRVMECEVLKEIARETGKSIAQVCLRWVYEQGVSLLVKSFNKERIKQNLEIFDWELSAQDLEKIIQIPQFKGFPGVEFVCQTGPYKSLVDLWDGEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AM30</accession>
    <name>A0AA39AM30_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_001274</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the amino acid/polyamine transporter 2 family. Amino acid/auxin permease (AAAP) (TC 2.A.18.2) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AM30"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000033">
        <property type="entry name" value="Lysine histidine transporter 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48017">
        <property type="entry name" value="OS05G0424000 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="147"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="170"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="257"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="296"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="342"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="368"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="402"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="10">
        <location>
            <begin position="25"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9709714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="435" mass="48871" checksum="A1A2EBD722428BB8" modified="2024-01-24" version="1">MEDEQKASRGKDIDNWLPITSSRDAKWWYSAFHNVTAMVGSGVLALPYAMAGLGWGPGVVVLILSWIITLYTLWQMVEMHEMVPGKRFDRYHELGQHAFGEKLGLWVVVPQQLIVEVGVDIVYMVTGGKSLQKFYNIVCSECQRLRTIYFIMIFASCHFVLSHLPNFNSISGVSFSAAAMSLTYSTIAWIGSAHKGVIPDVDYKYKSSSTPGKCFHFFHALGEVAFAYAGHNVVLEIQATIPSTPEKPSKGPMWKGVMFAYFIVAICYFPVALVGYRVFGNSVADNILITLEKPGWLIASANIFVVIHVIGSYQIYAIPVFDMMETFLVKKLNFTPCFRLRLITRTAYVAFTMFIAMMIPFFGSLMAFLGGLAFAPTTYFLPCIMWLAVYKPKRFSLSWFSNWTCITLGVVLMILAPIGALRQIIIQSKTYKLFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AM56</accession>
    <name>A0AA39AM56_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_000689</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AM56"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33132">
        <property type="entry name" value="OSJNBB0118P14.9 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33132:SF92">
        <property type="entry name" value="SERINE-RICH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="116"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9708794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="133" mass="14273" checksum="5FF4F7B1502F3A76" modified="2024-01-24" version="1">MLPTDPSSPTPNFIHCSPYLRLKEGLDSLGKFFVELQAIPEPLGDDKQGYEAEIPAVAEGSGVPVLTRQISNAKTNCLCSPTTHAGSFRCRLHRAPSLQRTKSIDSASQRESFPKANTASGNTTNNASTVEAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AMR0</accession>
    <name>A0AA39AMR0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NTF2-related export protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_001379</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Facilitates protein transport into the nucleus. Interacts with various nucleoporins and with Ran-GDP. Could be part of a multicomponent system of cytosolic factors that assemble at the pore complex during nuclear import.</text>
    </comment>
    <comment type="function">
        <text evidence="5">Has a role in nuclear-cytoplasmic transport of proteins and mRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Interacts with RAN1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus envelope</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AMR0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00780">
        <property type="entry name" value="NTF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.50:FF:000005">
        <property type="entry name" value="Nuclear transport factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045875">
        <property type="entry name" value="NTF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002075">
        <property type="entry name" value="NTF2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018222">
        <property type="entry name" value="Nuclear_transport_factor_2_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12612">
        <property type="entry name" value="NUCLEAR TRANSPORT FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02136">
        <property type="entry name" value="NTF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50177">
        <property type="entry name" value="NTF2_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="97"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="126"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="NTF2" evidence="7">
        <location>
            <begin position="6"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00058161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00062736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU369002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9709872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="145" mass="16024" checksum="18D1B54A455CF8DD" modified="2024-01-24" version="1">MDPDAVAKAFVEHYYSTFDANRANLANLYQESSMLTFEGQKIQGSPNIVAKLTSLPFQQCQHSITTVDCQPSGPAGGMLVFVSGNLQLAGEQHALKFSQLGISHLICVFIFYMIPFVERYKFVSNVYFLCLLACALNFVLGSILI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AQS1</accession>
    <name>A0AA39AQS1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_001250</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AQS1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31623">
        <property type="entry name" value="F21J9.9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31623:SF17">
        <property type="entry name" value="F21J9.9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9709679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="108" mass="12197" checksum="0F51F08ACBD28299" modified="2024-01-24" version="1">MGEIKVEVISRDTIKPSSPTPAHLRHFQLSFLDQTLTPTLMPIILFYPMDGDVKKTLSKTLIQFYPLAGRVEDNLFIDCSDQGIPYLEARPKSSANFRSSHATWTPCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D548</accession>
    <name>A0AA39D548_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Scarecrow-like transcription factor PAT1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_025788</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the GRAS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D548"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005202">
        <property type="entry name" value="TF_GRAS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31636">
        <property type="entry name" value="OSJNBA0084A10.13 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03514">
        <property type="entry name" value="GRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50985">
        <property type="entry name" value="GRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="76"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Leucine repeat I (LRI)" evidence="3">
        <location>
            <begin position="173"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="VHIID" evidence="3">
        <location>
            <begin position="252"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Leucine repeat II (LRII)" evidence="3">
        <location>
            <begin position="333"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="SAW" evidence="3">
        <location>
            <begin position="471"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="VHIID" evidence="3">
        <location>
            <begin position="283"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="76"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9672308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="545" mass="60546" checksum="D75D395A43A9FD2B" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D5D6</accession>
    <name>A0AA39D5D6_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_026853</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D5D6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20653">
        <property type="entry name" value="CYP81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000023">
        <property type="entry name" value="Cytochrome P450 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050651">
        <property type="entry name" value="Plant_Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47947">
        <property type="entry name" value="CYTOCHROME P450 82C3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47947:SF62">
        <property type="entry name" value="CYTOCHROME P450, FAMILY 81, SUBFAMILY D, POLYPEPTIDE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-0349">Heme</keyword>
    <keyword evidence="8 11" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041297327" evidence="12">
        <location>
            <begin position="21"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="11">
        <location>
            <position position="433"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9670570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="500" mass="56820" checksum="0A121555F7E4AE1F" modified="2024-01-24" version="1">MFCTALFIFCLILLKVFVRGRRRHRNLPPSPPPLPIIGHLHLLKGGGLHRTFRSLSEKYGPILFLQLGSQPTVIVSSPSVVEECFTKNDTVLANRPLFLLGKYLGYNFTALAWAGYGDHWRNLRRLSTLEVFSPSRLDLIVGIQKDEVKCLLQRLSPDSRDGFGKVELKSKFSELTFNIITRAVAGKRFYGEDVDAEVSVNFRNLINEIFQSAEATNPADLLPIFRWIDYQGFERKMIEVSRKSDVFMQGLIDEHRSDRSSLESRNTMIDHLLSLQKSQPEYYTDEIIKGLIMVLILAGTETSATTTEWAMALLLNHPNSLKKAIAEIDDRVGPERIMDETDLPNLPYLQNIVRETLRLYPPGPLLVPHVASEECEIGGYHIPKHTMLMVNAWAIQRDPKLWQDATSFRPERFETGKAETYKFLPYGVGRRACPGASMANRLIGLTLGTLIQCYIWERVSDKEVDMSGAEGLTMPKKTPLEAMCKPRDVLKKVFEEYKTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D6N9</accession>
    <name>A0AA39D6N9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_026401</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9673106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D6N9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF78">
        <property type="entry name" value="OS12G0587133 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase zinc-binding" evidence="1">
        <location>
            <begin position="378"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9673106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="539" mass="62184" checksum="4578E9E73F2AC5A5" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D8C9</accession>
    <name>A0AA39D8C9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF829 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_023789</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D8C9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008547">
        <property type="entry name" value="DUF829_TMEM53"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12265:SF0">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12265">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05705">
        <property type="entry name" value="DUF829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9674454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="408" mass="46008" checksum="4B73E41A0F931425" modified="2024-01-24" version="1">MWGFGGKFYWGRNETGGKIEGIVVVFAWMSSQEKHLKNYVQLYSSLGWNSLICHPEFLNVFFPEKATSLAADILNELVKELKTRPCPVVFASFSGGPKACMYKVLQIIDGKCEAQLNPDEYRLVRDSVAGHIYDSSPADFTSDVGTRFLLKPTVLKVSHPPRLVSWIANGIASSLDALFLNRFESQRAEYWQTLYSSVFMGAPYLILCSEDDDLAPYQIICNFAQRLQELGGDVKLVKWNSSPHVDHYRYHMIDYKAAVTELLEKAAIIYSQRIRRLEAERKSLEVHDETSNPICQPKKATTSSNECFGRVALELNDHFISPSSLEYQEGRNVGSVRNEHKEGLIHLSSLPIINAHGVLGQVLFDVCVPKDVEDWDIRSSGSSSAPASAQTRKHTPFNPIRCIRRSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D8E6</accession>
    <name>A0AA39D8E6_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_024552</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D8E6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33696:SF1">
        <property type="entry name" value="T22J18.15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33696">
        <property type="entry name" value="T22J18.15-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="22"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9675683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="199" mass="22150" checksum="A1066C845F54E537" modified="2024-01-24" version="1">MDSSTKILPNEGDTKFPATNQLSSRRSQSLSSLSSCSSSPESSFSGDSPTSPVTPPQFSGIPFSWEKIPGIPKKQTSRKKENSPLSLLPLPPSTTPIPSKRFNHQDQETSMIRKKKNISESFRRDPFFAAFVECCKEDEDQETSPNIWKSSKVPRNLSDRLGLINIYTSCKSTCTVSESIIFVPRSARSSYHLLNRRSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D9Q6</accession>
    <name>A0AA39D9Q6_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_023736</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D9Q6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000008">
        <property type="entry name" value="Tryptamine hydroxycinnamoyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051283">
        <property type="entry name" value="Sec_Metabolite_Acyltrans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31896">
        <property type="entry name" value="FAMILY REGULATORY PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G14730)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31896:SF12">
        <property type="entry name" value="HXXXD-TYPE ACYL-TRANSFERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9674362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="454" mass="50636" checksum="4B29B545119E7EB1" modified="2024-01-24" version="1">MATPTVRLISECFIKPKFTSEETKEPCYLTPWDLAMLSVNYIQKGLLFSKPPAVDDPQNFMATLLDRLKDSLALTLDHFYPLAGRLATKKEDNPPSYVVFVDCNNSPGAKFIHAAADMTVSDILSPIYVPQVIQSFFDHDRVINHDGHTLSLLSIQVTELVDGIFIGCSINHSMVDGTSFWHFFNAWSEVFTAQEKNSSISLSRPPILKRWFPDGHGPIINLPFTHHDQFISRFEAPVLRERIFHFSSESIAKLKAKANAECNSNKISSLQALSALVWRSITRARCFPPEQETSCRLATGNRTRLDPPLPENYFGNSIQTVRGIATAGELLEHDLGWAAWLLHQAVVGHTDKAVRGWLESWFQSHFIYQLGLFFDPNSIMMGSSPRFNKYGIEFGLGKGLALRSGYAHKFDGKASCYPGREGGGSIDLEVCLPPNSMSALESDQEFMAAVSLSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DEE8</accession>
    <name>A0AA39DEE8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_021199</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DEE8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017887">
        <property type="entry name" value="TF_TCP_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005333">
        <property type="entry name" value="Transcription_factor_TCP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31072">
        <property type="entry name" value="TRANSCRIPTION FACTOR TCP4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31072:SF1">
        <property type="entry name" value="TRANSCRIPTION FACTOR TCP9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03634">
        <property type="entry name" value="TCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51369">
        <property type="entry name" value="TCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TCP" evidence="7">
        <location>
            <begin position="58"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="27"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="141"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9679192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="169" mass="17569" checksum="EC4D7AE9EEF6C5C9" modified="2024-01-24" version="1">MASPSNPNEENLSIEKPTTLEISEAFPAPSETVSKSSGVEGEQEISTKTAIALPKKRAVDRHVKVEGRGRRIRMSSDSTARLFELTRQLGLKSEGETIRWLLKNAEVDIIRATGSGTIPAIATSVGGTLVVPRVTTGEAAATTSNAGSGEGLSESCGLAPISSAAPRGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DEG8</accession>
    <name>A0AA39DEG8_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_020106</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9681041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DEG8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9681041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="93" mass="10135" checksum="CC4D227F4E16606B" modified="2024-01-24" version="1">MAIKILSITTLNPGRVVQIEAPSHEDLVQYKHEVGDITIVVASKSCSSEDLKAIRAYCHELDYSYEGPLSIEVPSAVKENTESTSGQKGTTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DFS9</accession>
    <name>A0AA39DFS9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glucose-6-phosphate dehydrogenase (NADP(+))</fullName>
            <ecNumber evidence="3">1.1.1.49</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_019055</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>D-glucose 6-phosphate + NADP(+) = 6-phospho-D-glucono-1,5-lactone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15841"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57955"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="EC" id="1.1.1.49"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glucose-6-phosphate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.49" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DFS9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004345">
        <property type="term" value="F:glucose-6-phosphate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009051">
        <property type="term" value="P:pentose-phosphate shunt, oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00966">
        <property type="entry name" value="G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001282">
        <property type="entry name" value="G6P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019796">
        <property type="entry name" value="G6P_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022675">
        <property type="entry name" value="G6P_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022674">
        <property type="entry name" value="G6P_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23429:SF0">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23429">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-DEHYDROGENASE G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02781">
        <property type="entry name" value="G6PD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00479">
        <property type="entry name" value="G6PD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00079">
        <property type="entry name" value="G6PDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00069">
        <property type="entry name" value="G6P_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0313">Glucose metabolism</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucose-6-phosphate dehydrogenase NAD-binding" evidence="9">
        <location>
            <begin position="4"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose-6-phosphate dehydrogenase C-terminal" evidence="10">
        <location>
            <begin position="121"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9683303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="359" mass="41331" checksum="693ABC144F136714" modified="2024-01-24" version="1">MTEKKIKYVSGSYDAEDGFRLLDKEIAKHEFSKNSQEGSSRRLFYLALPPSVYPSVCRMIKLCCMNKSNLGGWTRIVVEKPFGKDLESAEQLSAQIGELFDEPQIYRIDHYLGKELVQNLLVLRFANRMFMPLWNRDNIDNVQIVFREDFGTEGRGGYFDEYGIIRDIIQNHLLQVLCLVAMERPVSLKPEHIRDEKVKVLQSVLPITDDEVVLGQYEGYTDDPTVPDLSNTPTFASMILRIHNERWEGVPFILKAGKALNSRKAEIRIQFKDVPGDIYRCQRQGRNEFVIRLQPLEAIYMKLTAAWEIFTPLLHRIDNGEMKPIPYKPGSRGPSEADELLSKSGYVQTHGYIWIPPTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DH18</accession>
    <name>A0AA39DH18_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Disease resistance protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_016355</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9683816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DH18"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14798">
        <property type="entry name" value="RX-CC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.430:FF:000003">
        <property type="entry name" value="Probable disease resistance protein At5g66910"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038005">
        <property type="entry name" value="RX-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF1185">
        <property type="entry name" value="DISEASE RESISTANCE RPP8-LIKE PROTEIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="6">
        <location>
            <begin position="5"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="5">
        <location>
            <begin position="170"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="7">
        <location>
            <begin position="440"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="8">
        <location>
            <begin position="575"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="534"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="534"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="546"/>
            <end position="557"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9683816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="943" mass="108519" checksum="C18B53A6FB5F4334" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DHM3</accession>
    <name>A0AA39DHM3_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_017304</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DHM3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34370:SF2">
        <property type="entry name" value="GAG-POL POLYPROTEIN_RETROTRANSPOSON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34370">
        <property type="entry name" value="OS04G0600100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="103"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="214"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9685223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="238" mass="26853" checksum="DBE0AFC91DD291F6" modified="2024-01-24" version="1">MSLPSKVCLHQLKLLSHHQGFNPSIPRVPTFHKLNNFSQFHHHHRHLRFCSINGHAGASGMSNGHDYSTNHEDSSAKGENGEGELYTSKDLLKKLKRYGISGILSYGLLNTAYYLTTFLLVWFYIAPAPGRMGYWRQLKGKNLFQKVSFCTSFSKYLDIWNPIGRFVKIMAMVWAGSQVNKLVRAGGALALAPIVDRGLSWFTVKFKFESQGKAFMAIVGFCFGLALILFFIVTLLWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DKM4</accession>
    <name>A0AA39DKM4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_015767</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Nudix hydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DKM4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047631">
        <property type="term" value="F:ADP-ribose diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035529">
        <property type="term" value="F:NADH pyrophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04670">
        <property type="entry name" value="NUDIX_ASFGF2_Nudt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000016">
        <property type="entry name" value="nudix hydrolase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000015">
        <property type="entry name" value="Nudix hydrolase 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003293">
        <property type="entry name" value="Nudix_hydrolase6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040618">
        <property type="entry name" value="Pre-Nudix"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13994:SF26">
        <property type="entry name" value="NUDIX HYDROLASE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13994">
        <property type="entry name" value="NUDIX HYDROLASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18290">
        <property type="entry name" value="Nudix_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01356">
        <property type="entry name" value="GFGPROTEIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="4">
        <location>
            <begin position="116"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9687040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="291" mass="32473" checksum="39C2033DD3A55CBD" modified="2024-01-24" version="1">MSGSTTSMMVDEQRLPENNVQQIELLTGTEDSYGGVRVEIKNRMDSDVFGDVLRASISQWRQKGTKGVWIKLPIQHANLVEAAVKEGFWYHHAEPDYLMLVLWIPKTAHTLPANASHRVGIGAFVINSKGEVLVVQENSGRFKGTGVWKLPTGVANEGEDICTAAIREVEEETGIKTEFVEILAFRQSHKAFFTKSDLFFVCMLQPLSSEIQKQEEELEAAQWMAVEEYAAQPFVKKNPLFDSIANICLAKKDMKYTGFSPFSATSSSGKTNILYFNNQDLKQPLSSGNQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DNB0</accession>
    <name>A0AA39DNB0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF538 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_012700</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DNB0"/>
    <dbReference type="SMR" id="A0AA39DNB0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.240.10:FF:000002">
        <property type="entry name" value="Uncharacterized protein At3g07460"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.240.10">
        <property type="entry name" value="At5g01610-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036758">
        <property type="entry name" value="At5g01610-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007493">
        <property type="entry name" value="DUF538"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31676">
        <property type="entry name" value="T31J12.3 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31676:SF28">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04398">
        <property type="entry name" value="DUF538"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141562">
        <property type="entry name" value="At5g01610-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9690174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="171" mass="19328" checksum="BD0FFB7F0DBA5E73" modified="2024-01-24" version="1">MDSLLLLLLHHRHRPFAMSSMLTISAILVLLPFTNSYSIQDLLRSRGLPAGLFPNNVKSYNLDQRGRLEVYLEGPCMAKFENRVYFERVVTANLSYGGLIGVEGLTQEELFLWLPVKDIIVDDPNSGLILFDIGLAHKQLSLSLFEDPPVCKPQGVLEISGRKNKEFQTQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DNY8</accession>
    <name>A0AA39DNY8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_013773</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DNY8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050667">
        <property type="entry name" value="PPR-containing_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47939">
        <property type="entry name" value="MEMBRANE-ASSOCIATED SALT-INDUCIBLE PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47939:SF13">
        <property type="entry name" value="OS03G0201400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="9"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="44"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="79"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="114"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="149"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="184"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="219"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="260"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9691691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="318" mass="35650" checksum="A427BA30EC631D90" modified="2024-01-24" version="1">MRLKGIEPDSYTYGSLISGMCKEGRLEEASGLFDKMVEIGLVPNAVTHNTLIDGYCNKGDFKRAFSYRDEMVKKGIMPSVSTYNLLVHALFMEGRMGEADDMIKEMRKKGIIPDAITYNILINGYSRCGNAKKAFDLHNEMLSKGIEPTCVTHTSLIYVLSRRNRMKEADDLFEKILDQGVCPDVIMFNAMIDSHCANGNVEHALMLLKEMDRKSVPPDEVTFNTLMQGRCREGKVEEARMLLDEMKVQDEMLSIGFNPTLLTFNALIKCLCRNQDGDLAEELLKEMVNKGISPDDSTYLSLIKGIGNVDTLVEDNAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DQC5</accession>
    <name>A0AA39DQC5_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_012812</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DQC5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9690352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="228" mass="26373" checksum="3757352346A68781" modified="2024-01-24" version="1">MVEGYNFFMQPLVNIPRLNEEEDWRRTSIFQTRGVCQGKLCTLIIDGGKDHPNPYQIAWVNGTSILVSSHCLVTFNFNNNFELSAWCDENTYTLVHNGCNKILHPMKESPPHKEPKEKLASLKLEEPSNTLTKKQVKVTRKEEEIINDESRKQEVMELILDQPVEELKEVVEASKESMLNFPRPNIITSGGKHEEFVEISFEHRDLRILFFYKTVNKKSMGSNFPQAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DQW1</accession>
    <name>A0AA39DQW1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mal d 1-associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_009717</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9693881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DQW1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053346">
        <property type="entry name" value="Fra_a_1-associated"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35722">
        <property type="entry name" value="MAL D 1-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35722:SF1">
        <property type="entry name" value="MAL D 1-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9693881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="193" mass="21198" checksum="4E4AB9C48183CFC1" modified="2024-01-24" version="1">MGWVWEDDAGEANTAGDVTGFQNPNLRSEDRCSTRRIVKSQCRTEEVKPGKFVRKCQKTQQILKDCVGRPTEVVQSNEEYTEDDVTDLVVKGSAPLGLPEQGHFDFPGLRSDIEAIERNLFGGLNRFFEAAEEMKNGFFSVFGAPPVFDGESSSSSTRRGISFEGSNPTKEASAEPFKAESGYGDLSGLAREV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DRJ9</accession>
    <name>A0AA39DRJ9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_010212</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the AP2/ERF transcription factor family. AP2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DRJ9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000002">
        <property type="entry name" value="AP2-like ethylene-responsive transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000004">
        <property type="entry name" value="AP2-like ethylene-responsive transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32467">
        <property type="entry name" value="AP2-LIKE ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32467:SF226">
        <property type="entry name" value="AP2_ERF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00367">
        <property type="entry name" value="ETHRSPELEMNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="10">
        <location>
            <begin position="151"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="AP2/ERF" evidence="10">
        <location>
            <begin position="243"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="123"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="340"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="126"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="359"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9694631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="495" mass="54341" checksum="A16165F869CD08A7" modified="2024-01-24" version="1">MLDLNLNDVSDDSFHGSTQAVDPGKLLDLSSNQMEDSGSFNSSIVNADALSSTTADDDSSSNHPDHCCGIVAYSFDFFKQNEDHDNKNSDSVTRQFFPVTSGGEGDCSLGSSLQHPFILQVGSGGPLLPKKPQQQQQVKKSRRGPRSRSSQYRGVTFYRRTGRWESHIWDCGKQVYLGGFDTAHAAARAYDRAAIKFRGADADINFAVSDYDEDIKQMSNFTKEEFVHVLRRGSTGFSRGSSKYRGVTLHKCGRWEARMGQFLGKKYIYLGLFDSEVEAARAYDKAAIKCNGREAVTNFEPSTYEGEMTFPADNGGSSQNLNLLPNLDLHLGVAPYFSDEPMSDEPVRNSKANGSPSQEGHDAMPDDTGERIENSTSATLGVHPSYGLVMASNHPPIWSGVNSRVFPVYKERAIEKRIEVDHLPNWAWQIHGPYGGATPPIFSTAASSGFLSSVTTASPANPTQGSLQTTTVLQHYYSPSLTNTTSISPYYYCRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2025-06-18" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DSE5</accession>
    <name>A0AA39DSE5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_011517</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03233">
        <property type="entry name" value="ABCG_PDR_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03232">
        <property type="entry name" value="ABCG_PDR_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000179">
        <property type="entry name" value="ABC transporter G family member 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000059">
        <property type="entry name" value="ABC transporter G family member 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034001">
        <property type="entry name" value="ABCG_PDR_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034003">
        <property type="entry name" value="ABCG_PDR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013581">
        <property type="entry name" value="PDR_assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48040:SF20">
        <property type="entry name" value="PLEIOTROPIC DRUG RESISTANCE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48040">
        <property type="entry name" value="PLEIOTROPIC DRUG RESISTANCE PROTEIN 1-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08370">
        <property type="entry name" value="PDR_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="521"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="554"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="605"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="639"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="664"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="748"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1185"/>
            <end position="1203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1215"/>
            <end position="1234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1271"/>
            <end position="1291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1298"/>
            <end position="1322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1328"/>
            <end position="1347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1359"/>
            <end position="1379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1406"/>
            <end position="1429"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="152"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="839"/>
            <end position="1091"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9692497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1437" mass="162285" checksum="92C7BB7C5D5E3AE5" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DU11</accession>
    <name>A0AA39DU11_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycosyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_009378</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="5"/>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DU11"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080043">
        <property type="term" value="F:quercetin 3-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080044">
        <property type="term" value="F:quercetin 7-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000057">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000756">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926">
        <property type="entry name" value="GLUCOSYL/GLUCURONOSYL TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926:SF1560">
        <property type="entry name" value="UDP-GLYCOSYLTRANSFERASE 74E1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9697526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="456" mass="50557" checksum="200DF851D393E164" modified="2024-01-24" version="1">MEKEKRTHKAHCIILPYPSQGHINPMLQFSKRLVHNGAKVTLAATRFISKSLVGDSGPITIETISDGYDEGGSAQAESDGAYLERFQVVGSETLGSLIEKLKSSGCPVDCVVYDAFLPWALDVAKQLGLVGAVFFTQSCTVNDIYYHVHKGMLKLPLSEPEVVVPGLFPLQACDLPSFVYLYGSYPAFFDMVVNQFSNIEKVDWVFCNTFYKLEEKVVDWMAKICPLRTIGPTLPSAYLDKRLGDDKDYGLNMLKPVTGACMEWLDSKPKGSVVYASYGSFAVLEPEQLEEVAWGLRRSNAYFLMVVRESEQAKLPQNFKKETAEKGLVVSWCPQLEVLAHRAIGCFLTHGGWNSTLEALSLGVPMVVAPLWTDQPTNAKFVEDVWGIGLRARADDKGIVRREVLENCIGKVMGSDGLKEIKNNAMKWKNLAREAVDEGGSSDKCIDEFVAKLAVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DUL1</accession>
    <name>A0AA39DUL1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Protein transport protein Sec61 subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_007315</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SEC61-beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DUL1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005784">
        <property type="term" value="C:Sec61 translocon complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030671">
        <property type="entry name" value="Sec61-beta/Sbh"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016482">
        <property type="entry name" value="SecG/Sec61-beta/Sbh"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13509">
        <property type="entry name" value="SEC61 SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03911">
        <property type="entry name" value="Sec61_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="73"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="42"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9698171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="107" mass="10766" checksum="626550C2115C932F" modified="2024-01-24" version="1">MARGSSQSQSSTTATSRPGPVGMAPRGSAAATAGMRRRRVGSGSGGTSGGYGGGSSGSNMLRFYTDDAPGLKITPTVVLVMSLCFIGFVTALHVFGKIYRYKAGGGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DVE1</accession>
    <name>A0AA39DVE1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Translation elongation factor EF1B beta/delta subunit guanine nucleotide exchange domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_007906</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">EF-1 is composed of 4 subunits: alpha, beta (1B-alpha=beta'), delta (1B-beta), and gamma (1B-gamma).</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the EF-1-beta/EF-1-delta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9699056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DVE1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005853">
        <property type="term" value="C:eukaryotic translation elongation factor 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00292">
        <property type="entry name" value="EF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.60:FF:000001">
        <property type="entry name" value="Elongation factor 1-beta 1 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036219">
        <property type="entry name" value="eEF-1beta-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049720">
        <property type="entry name" value="EF1B_bsu/dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014038">
        <property type="entry name" value="EF1B_bsu/dsu_GNE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014717">
        <property type="entry name" value="Transl_elong_EF1B/ribsomal_bS6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001326">
        <property type="entry name" value="Transl_elong_EF1B_B/D_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11595">
        <property type="entry name" value="EF-HAND AND COILED-COIL DOMAIN-CONTAINING FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11595:SF84">
        <property type="entry name" value="ELONGATION FACTOR 1-BETA 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00736">
        <property type="entry name" value="EF1_GNE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00888">
        <property type="entry name" value="EF1_GNE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54984">
        <property type="entry name" value="eEF-1beta-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00824">
        <property type="entry name" value="EF1BD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00825">
        <property type="entry name" value="EF1BD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="5 6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Translation elongation factor EF1B beta/delta subunit guanine nucleotide exchange" evidence="8">
        <location>
            <begin position="137"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="99"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9699056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="226" mass="24478" checksum="C411872CD6F6162E" modified="2024-01-24" version="1">MAITFSDLHTEAGLKSLDVFLSGKAYISGDHLTKDDVKVYAAVLEKPGSHFPNASKWYSCVSSHLAKSFPGKAVGVRVGCQGAPVEAAPKEVKEAAPAGDDDDDLDLFGDETEEEKKAAEEREAAVKASSKKKESGKSSVLLDVKPWDDETDMKKLEEAVRSIEMPGLLWGASKLAPVGYGIKKLQIMMTIVDDLVSVDSLIEERLTVEPISEYVQSCDIVAFNKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DWB6</accession>
    <name>A0AA39DWB6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_008091</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9699323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DWB6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000096">
        <property type="entry name" value="Basic helix-loop-helix (BHLH) DNA-binding superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051358">
        <property type="entry name" value="TF_AMS/ICE1/BHLH6-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31945:SF17">
        <property type="entry name" value="TRANSCRIPTION FACTOR FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31945">
        <property type="entry name" value="TRANSCRIPTION FACTOR SCREAM2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="8">
        <location>
            <begin position="125"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="102"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="111"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9699323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="316" mass="35229" checksum="029096A2F1673B50" modified="2024-01-24" version="1">MDRRDASAGTLANVTDFGFHDFINEPNFEQLIELIRGESADSIVKFCPNYDCEHMNGCFDDNQFGSSVGELFEFDPATATATASNPDSVIDSLPSIDGEMKEGEEIDGEDSSGNTTTTPTKGTKVDRSRTLISERRRRVRMKEKLYALRSLVPNITKMDKASIVGDAVLYLQQLQMQAKKLKAEIAGLESSLVLGKERYNGLVEIPKKTQVARSHHPMCGKIFQMDVFQVEERGFYVRLGCNRGERVAVSLYKALESLTGFNIQSSNLATFSETFVLTFTLNVRECDESMNLPNLKLWLTGALLNQGFEFKTLPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DZD5</accession>
    <name>A0AA39DZD5_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_006390</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DZD5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9701020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="143" mass="16926" checksum="67AD8AB206B67714" modified="2024-01-24" version="1">MSPYRLVYGKACHLPMEIEYKAWWAIKKLNMDLTRAGLKRCLDLNELEEMRNDAYLNSKISKERLKKWHDQLVNQKNFAKGQRVLLYDSKLHLFPGKLKSRWTGPFIIHDVQPNGVVELLNFNSTRTFKVNGHRLKPYMESFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E1R1</accession>
    <name>A0AA39E1R1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Calmodulin-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_003162</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E1R1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012442">
        <property type="entry name" value="DUF1645_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33095">
        <property type="entry name" value="OS07G0619500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33095:SF81">
        <property type="entry name" value="OS07G0619500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07816">
        <property type="entry name" value="DUF1645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="298"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="159"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="181"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="203"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="298"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9704978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="365" mass="40398" checksum="1D23BD347B38B7EE" modified="2024-01-24" version="1">MEMEVVIPVPAVDFNFDSACSTPFISAPSSPQRFGNFSSAPTSPSRASAFYREFNDFNFSGRDSSASAASVIPFDWEEKPGIPKVKTAATRSTTRDEYDDDQDFAFDFSGQLERTSLSADELFDGGKIRPLRPPPRLQLSNNNPDENFKSAISSPKSPRSPKKMFSPRHRKDTDPFAAAIERTRKEAGTEPQYHRGRERKTGSSSSSSATSTAATSRKVTRSLSPLRVSDITFGSEHSVPQNINNSKLSSSSSKSSRIWRLRDFLLFRSASEGRAASKDPLRKYSLLTKKNEDVKNCSFRSTDSSGSVSSSRRRGPVSAHEMHYTVNRAVSEEMRRKTFLPYKQGLLGCLGFNSTVHEISKGLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E1S6</accession>
    <name>A0AA39E1S6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pectinesterase inhibitor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_005368</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the PMEI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9704049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E1S6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004857">
        <property type="term" value="F:enzyme inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14859">
        <property type="entry name" value="PMEI_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.40:FF:000011">
        <property type="entry name" value="Cell wall / vacuolar inhibitor of fructosidase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.40">
        <property type="entry name" value="Invertase/pectin methylesterase inhibitor family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035513">
        <property type="entry name" value="Invertase/methylesterase_inhib"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006501">
        <property type="entry name" value="Pectinesterase_inhib_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01614">
        <property type="entry name" value="PME_inhib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35357:SF8">
        <property type="entry name" value="OS01G0111000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35357">
        <property type="entry name" value="OS02G0537100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04043">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00856">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101148">
        <property type="entry name" value="Plant invertase/pectin methylesterase inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041429865" description="Pectinesterase inhibitor domain-containing protein" evidence="5">
        <location>
            <begin position="22"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="Pectinesterase inhibitor" evidence="6">
        <location>
            <begin position="28"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9704049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="182" mass="20050" checksum="EDDC8FEB5BC6B594" modified="2024-01-24" version="1">MGSTGLLFLFLLMSLLQFFHPQLVLVSGDYDLIQKTCRSTKYYDLCISSLKSDPNSPNADTKGLAMIMVGIGEANATAISSYLSSELLGSANDTSMKKILKECVNRYTYSSDALQASLQSLAMEAYDYAYVHVIAAADYPNACRNAFKRCPRLAYPPELGPREDGLKHLCDVVLGIINLLDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E1Y0</accession>
    <name>A0AA39E1Y0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Neprosin PEP catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_001847</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E1Y0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053168">
        <property type="entry name" value="Glutamic_endopeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004314">
        <property type="entry name" value="Neprosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025521">
        <property type="entry name" value="Neprosin_propep"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31589:SF223">
        <property type="entry name" value="PROTEIN, PUTATIVE (DUF239)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31589">
        <property type="entry name" value="PROTEIN, PUTATIVE (DUF239)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03080">
        <property type="entry name" value="Neprosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14365">
        <property type="entry name" value="Neprosin_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52045">
        <property type="entry name" value="NEPROSIN_PEP_CD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041439614" description="Neprosin PEP catalytic domain-containing protein" evidence="1">
        <location>
            <begin position="25"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="Neprosin PEP catalytic" evidence="2">
        <location>
            <begin position="150"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS52045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9706571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="393" mass="43870" checksum="C38C5F5EC5E28ACE" modified="2024-01-24" version="1">MGLKVVLPCLLVCYLFLYYNGVEGYRKLSKEEDLEIEKELKRLNKPAVKTIKTKYGDIYDCVDFYKQPAFDHPLLKNHNFHPQMMPTSPPRRVSPEKEVPKPDYKQVKLGLEGGGCPMGTVPIRRTTKDDLITAKLYSEMHASKINPLTDEEPGKHFAVSRTTENIDYDGVGAMLSVWNLPAKAPQYTSGRVKIKNGAESLEAGWTVNPGLYGGDTRTRMYIYTNAGQAHCFNTQCGYIQASTDIPVDMVVEPVSTYGQRPYYITLSIYQDTINLNWYLKYDDNRTVIGWWPSRIFTNLGSTATGAEWGGEVFSPPNVPSPAMGSGHRLKLDTNYDAFCAQANIVVNNTIIKPPANLRQFADSFNFDITNRRDVGGDPGYLILYGGVSGATGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E505</accession>
    <name>A0AA39E505_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase Ty1/copia-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_002350</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E505"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439:SF475">
        <property type="entry name" value="CYSTEINE-RICH RLK (RECEPTOR-LIKE PROTEIN KINASE) 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439">
        <property type="entry name" value="GAG-POL-RELATED RETROTRANSPOSON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase Ty1/copia-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9707319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="282" mass="32241" checksum="104055F56753D5AD" modified="2024-01-24" version="1">MDVKNAFLHGELDREIYMNQPMGFQSEGHPEYVCKLWKALYGLKQAPRAWYGKIAEFLTQSGYSVTPVDSSLFVKANGGKLAIVLVYVDDLIITGDDVEEICRTKENLSVRFEMKELGQLKLFLGLKMDRTHEGIFLCQQKYAKDLLKKFGMLECKPISILMEPNAKMCEHEGKDLKDATMYRQLVGSLLYLTLTRPDISYAVGVMSRYMQNPKKPHLEAIRRILRHVKGTIDYGLLYKKGEDCKLVGYCDADYAGDHDTRRSTTRYVFMLGSGAISWCSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ED85</accession>
    <name>A0AA39ED85_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_001256</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ED85"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31623:SF118">
        <property type="entry name" value="BAHD ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31623">
        <property type="entry name" value="F21J9.9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9709687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="429" mass="48098" checksum="2690D33C6A893DB7" modified="2024-01-24" version="1">MEIMKPEIISRQTIKPSSPTPDSKKIYKLSLLDLLNAHRIYVPIIFFYQNKEGDNSVDVAHISSGLKKSLSETLTHYYPLAGRIKDGVTVECNDEGAYFSEARIDCHLEGFLKHPEARDDLDLLMPVEIQSNSSEMGSLLLIQINFFSCGGIAIGIRVSHKVSDLHSISAFLNDWATMARKSGEEISAELTPFFPPGDSLSMPEAEPYKGNLIKRRFVFDAVKLGALKAMATQSGVENPTRVEVVAALIYRCAVAASKVSSGSLKPSVLLQSVNLRKRIVPPLPEKSFGNMVWFYRIHTARESEIEFHALVGQLKAGLAHFCDTYGKNFTGQELIRLMFKERAELTHSNDNHHVNKYICTSWCRTSLYQTDFGWGKPIWLRPTGIGSKNIICLIDTREGDGIEAFVTLEEQEMAVFERDEELLQFSSLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YGC9</accession>
    <name>A0AA38YGC9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TF-B3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_026486</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9669947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YGC9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10017">
        <property type="entry name" value="B3_DNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.330.10">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003340">
        <property type="entry name" value="B3_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015300">
        <property type="entry name" value="DNA-bd_pseudobarrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044837">
        <property type="entry name" value="REM16-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31391:SF155">
        <property type="entry name" value="B3 DOMAIN-CONTAINING PROTEIN OS11G0197600"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31391">
        <property type="entry name" value="B3 DOMAIN-CONTAINING PROTEIN OS11G0197600-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02362">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01019">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101936">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50863">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TF-B3" evidence="6">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9669947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="104" mass="11973" checksum="85974599DF40EA51" modified="2024-01-24" version="1">MGPSYLGRSRHGLNIPMWFVERYFKTDDKCITLWGSDGRTWSTSYRLGRRSCGKRIAELLSSGWKIFVQDNQLKFGDVCVFVLIKSPGILLKVVIFRNSEDADH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YHP9</accession>
    <name>A0AA38YHP9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">WRKY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_026911</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YHP9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.80:FF:000004">
        <property type="entry name" value="WRKY transcription factor 65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.80">
        <property type="entry name" value="WRKY domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003657">
        <property type="entry name" value="WRKY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036576">
        <property type="entry name" value="WRKY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044810">
        <property type="entry name" value="WRKY_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018872">
        <property type="entry name" value="Zn-cluster-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31282">
        <property type="entry name" value="WRKY TRANSCRIPTION FACTOR 21-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10533">
        <property type="entry name" value="Plant_zn_clust"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03106">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00774">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118290">
        <property type="entry name" value="WRKY DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50811">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="WRKY" evidence="7">
        <location>
            <begin position="259"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="41"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="95"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="179"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="44"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="179"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="202"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9670649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="337" mass="36770" checksum="82028A351E1DCFB7" modified="2024-01-24" version="1">MAVELMMGYRNDGFAAKIEENAVQEAAAGLESVEKLIRLLSHGQQQQSQQQPQPQQQLGRSSGEMEMDCRAVADVAVSKFKKVISLLGRTRTGHARFRRAPLSSPPNQSEETQGGGSRVYCPVPIQQVPPVPIQNQHQPHNDPPMVFARNGVIDRKDSTTTINFSYSSAISANSFMSSLTGDTDSKQPSSSSAFQITNLSQVSSVGRPPLSSSSMKRKCSSSDNPGSGKCGGSSGRCHCSKRRKMKLKRVVRVPAISMKMADIPPDDFSWRKYGQKPIKGSPHPRGYYKCSSVRGCPARKHVERALDDPKMLIVTYEGEHNHSLNVAEAAGLILESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YHV3</accession>
    <name>A0AA38YHV3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Acetyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_026934</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YHV3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051283">
        <property type="entry name" value="Sec_Metabolite_Acyltrans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31896">
        <property type="entry name" value="FAMILY REGULATORY PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G14730)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31896:SF43">
        <property type="entry name" value="PROTEIN ENHANCED PSEUDOMONAS SUSCEPTIBILITY 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9670701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="295" mass="33016" checksum="3AFF4A2EEDE7B063" modified="2024-01-24" version="1">MAPEFSLFTKPLMALPWGTSWDPVYVPRIVSSFFPLDGTRNCYGVSEPLLAVQVTELVDDIFIGCTMNHVVADGTSFWHFFNSWSGISRGSNKIFLSPVFKRWFMPHTHFPIRIPQSALVFGRPPSSPTQERVMGTNEKGKNFHFTNEKIAMLKVRGNVEMGTNEIFSLQALLAHLCMYFGVAIQPGFTTMKADGLLGQGLGHAAWQMNRMIATYTYTEAEATNFLESWVKNPKPFPPRNTTTNDILYMTGSHWFNVYGTDFGWGSPVAVRCGGGNRYDGRTTSSQGAEEVSNEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YI47</accession>
    <name>A0AA38YI47_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transcription factor CBF/NF-Y/archaeal histone domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_027032</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NFYB/HAP3 subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YI47"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016602">
        <property type="term" value="C:CCAAT-binding factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22907">
        <property type="entry name" value="HFD_NFYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000035">
        <property type="entry name" value="Nuclear transcription factor Y subunit B-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027113">
        <property type="entry name" value="Transc_fact_NFYB/HAP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003956">
        <property type="entry name" value="Transcrpt_fac_NFYB/HAP3_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11064">
        <property type="entry name" value="CCAAT-BINDING TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11064:SF150">
        <property type="entry name" value="NUCLEAR TRANSCRIPTION FACTOR Y SUBUNIT B-11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00615">
        <property type="entry name" value="CCAATSUBUNTA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00685">
        <property type="entry name" value="NFYB_HAP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription factor CBF/NF-Y/archaeal histone" evidence="7">
        <location>
            <begin position="27"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="189"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="9"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="193"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9670848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="208" mass="22874" checksum="2F81C5CBBDB5B3DE" modified="2024-01-24" version="1">MTGKKAQNSPVGSPSSDTSSSKEQDRFLPIANVSRIMKKSLPANAKISKEAKETVQECVSEFISFITGEASDKCQREKRKTINGDDLLWAMTMLGFENYVGPLKVYLSKYRETEGEKNNVVARHEDQSAVFNSNQMNKANKSIPINPDFQGFNGGFYSLGPQMNGDEAAAAMGYGDSLVTGAFNRSRIRENDGNGNRTMASYSSPQWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YIG5</accession>
    <name>A0AA38YIG5_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_027151</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9671030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YIG5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9671030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="47" mass="5031" checksum="192AA536A7DE2164" modified="2024-01-24" version="1">MGGYGRQPFVMRGGFDGVHGEPDYVDAALITIFQIHLEEGPGGILVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YJ29</accession>
    <name>A0AA38YJ29_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">PVL29_025180</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="23">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YJ29"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000217">
        <property type="entry name" value="probable LRR receptor-like serine/threonine-protein kinase At1g53430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000838">
        <property type="entry name" value="Probable LRR receptor-like serine/threonine-protein kinase At1g53440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000916">
        <property type="entry name" value="Probable LRR receptor-like serine/threonine-protein kinase At1g53440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.430:FF:000004">
        <property type="entry name" value="Putative leucine-rich repeat receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000044">
        <property type="entry name" value="Putative LRR receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000433">
        <property type="entry name" value="Putative LRR receptor-like serine/threonine-protein kinase isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.430">
        <property type="entry name" value="Galactose-binding lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051824">
        <property type="entry name" value="LRR_Rcpt-Like_S/T_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021720">
        <property type="entry name" value="Malectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48006">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN DDB_G0281931-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48006:SF60">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11721">
        <property type="entry name" value="Malectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="14 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8 21" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041446370" description="non-specific serine/threonine protein kinase" evidence="21">
        <location>
            <begin position="25"/>
            <end position="1025"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="603"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="664"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="971"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="971"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="999"/>
            <end position="1008"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAJ9671360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1025" mass="113848" checksum="6C7CF9846305F6FB" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YJK0</accession>
    <name>A0AA38YJK0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TOD1/MUCI70 glycosyltransferase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_025375</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YJK0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006852">
        <property type="entry name" value="TOD1_MUCI70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048354">
        <property type="entry name" value="TOD1_MUCI70_glycTrfase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12956">
        <property type="entry name" value="ALKALINE CERAMIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12956:SF61">
        <property type="entry name" value="TRNA (MET) CYTIDINE ACETYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04765">
        <property type="entry name" value="TOD1_MUCI70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="TOD1/MUCI70 glycosyltransferase-like" evidence="3">
        <location>
            <begin position="217"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="553"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="571"/>
            <end position="594"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9671640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="604" mass="68515" checksum="62DBEB209C851C12" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YJX4</accession>
    <name>A0AA38YJX4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Apple domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_025481</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YJX4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048544">
        <property type="term" value="P:recognition of pollen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01098">
        <property type="entry name" value="PAN_AP_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000858">
        <property type="entry name" value="S_locus_glycoprot_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32444">
        <property type="entry name" value="BULB-TYPE LECTIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32444:SF247">
        <property type="entry name" value="OS01G0958200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08276">
        <property type="entry name" value="PAN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00954">
        <property type="entry name" value="S_locus_glycop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00473">
        <property type="entry name" value="PAN_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Apple" evidence="3">
        <location>
            <begin position="107"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9671789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="215" mass="24438" checksum="0176223D8B6F28BA" modified="2024-01-24" version="1">MFVTDYEGGMLYFTYSRYNDTFPSILIMNYTGIFEKLEWIEERKEWSSVWEAPDGRCVYGICGPFGICSESESPICRYLNGFEPKFLDEWSKGDWSGGCVRRTPLQCEKNSITSKGRKGDGFLKLEGLKLPDFADFLSDVSSEECEESCLRNCSCVAYSYTSGIGCMIWHGSILDDQEFSIGGEKLFLRLAEVELGNSSTPNLLAQKQRELVKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YK54</accession>
    <name>A0AA38YK54_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_025553</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YK54"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010333">
        <property type="term" value="F:terpene synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016102">
        <property type="term" value="P:diterpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00684">
        <property type="entry name" value="Terpene_cyclase_plant_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.600.10:FF:000007">
        <property type="entry name" value="Isoprene synthase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.130:FF:000001">
        <property type="entry name" value="Isoprene synthase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.130">
        <property type="entry name" value="Terpene synthase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034741">
        <property type="entry name" value="Terpene_cyclase-like_1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044814">
        <property type="entry name" value="Terpene_cyclase_plant_C1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001906">
        <property type="entry name" value="Terpene_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036965">
        <property type="entry name" value="Terpene_synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050148">
        <property type="entry name" value="Terpene_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005630">
        <property type="entry name" value="Terpene_synthase_metal-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225:SF221">
        <property type="entry name" value="(-)-GERMACRENE D SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225">
        <property type="entry name" value="OS04G0344100 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01397">
        <property type="entry name" value="Terpene_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03936">
        <property type="entry name" value="Terpene_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01019">
        <property type="entry name" value="Terpene_Cyclase_Like_1_C_Termi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01604">
        <property type="entry name" value="Terpene_Cyclase_Like_1_C_Termi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Terpene synthase N-terminal" evidence="5">
        <location>
            <begin position="31"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="Terpene synthase metal-binding" evidence="6">
        <location>
            <begin position="260"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9671959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="556" mass="64193" checksum="75CFCB77FC637654" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YKX8</accession>
    <name>A0AA38YKX8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cytochrome P450 71D10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_025826</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YKX8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11072">
        <property type="entry name" value="CYP71-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000008">
        <property type="entry name" value="Cytochrome P450 71D8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47955:SF8">
        <property type="entry name" value="CYTOCHROME P450 71D11-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47955">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 71 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8 9" id="KW-0349">Heme</keyword>
    <keyword evidence="6 8 9" id="KW-0408">Iron</keyword>
    <keyword evidence="4 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="8">
        <location>
            <position position="445"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9672358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="508" mass="57319" checksum="7C322540BF09E758" modified="2024-01-24" version="1">MELQFSFFSILCTFLLFIYLLKRLGKPSRTTHPPPNLPPGPWKLPIIGNMHQLVGSLPHHSLRNLAKKHGPLMHLQLGEVSAIVVSSREMAKEVMKTHDIIFSQRPCILAASIVSYDCTDIAFAPYGGYWRQIRKISILELLSAKRVQSFRSVREEEVLNLVKSISSQEGVSINLTESIFSLTFSIISRAAFGKKCKDQEAFSVTLEKFADSGGGFTIADVFPSIKLLHVVSGIRQKLEKIHKKLDTILENIINEHKARSAASEISEAEVDEDLVDVLLKVQKQGDLEFPLTTDNIKAILLDLFIAGSETSSTAVEWAMAEMLKNPGVMAKAQAEVRDIFSRKGNADETMIHELKFLKLVIKETLRLHPPVPLLIPRESRESCAINGYEIPVKTRVIINAWAVARDPEHWNAAESFNPERFLDSSIDYQGTNFEYIPFGAGRRMCPGILFGMANVEIALAQLLYYFDWKLPNGIQHEELDMTEDFRTSLRRKLNLHLIPITYRPLPVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YLQ5</accession>
    <name>A0AA38YLQ5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cationic amino acid transporter C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_026119</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. Cationic amino acid transporter (CAT) (TC 2.A.3.3) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YLQ5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000035">
        <property type="entry name" value="Cationic amino acid transporter 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029485">
        <property type="entry name" value="CAT_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43243:SF62">
        <property type="entry name" value="CATIONIC AMINO ACID TRANSPORTER 8, VACUOLAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43243">
        <property type="entry name" value="INNER MEMBRANE TRANSPORTER YGJI-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13906">
        <property type="entry name" value="AA_permease_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="83"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="126"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="206"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="274"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="322"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="396"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="431"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="456"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="487"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="domain" description="Cationic amino acid transporter C-terminal" evidence="9">
        <location>
            <begin position="462"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9672768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="520" mass="57585" checksum="AD1F07EAD824EFCF" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YNK7</accession>
    <name>A0AA38YNK7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">STAS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_023369</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YNK7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008271">
        <property type="term" value="F:secondary active sulfate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07042">
        <property type="entry name" value="STAS_SulP_like_sulfate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.750.24:FF:000002">
        <property type="entry name" value="Sulfate transporter 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="entry name" value="STAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018045">
        <property type="entry name" value="S04_transporter_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011547">
        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001902">
        <property type="entry name" value="SLC26A/SulP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00815">
        <property type="entry name" value="sulP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11814">
        <property type="entry name" value="SULFATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01740">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00916">
        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SpoIIaa-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01130">
        <property type="entry name" value="SLC26A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="72"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="150"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="208"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="263"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="288"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="381"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="406"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="437"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="478"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="domain" description="STAS" evidence="7">
        <location>
            <begin position="547"/>
            <end position="654"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9673773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="664" mass="72216" checksum="C5F6F1841DE36729" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YPJ0</accession>
    <name>A0AA38YPJ0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_023628</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YPJ0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF78">
        <property type="entry name" value="OS12G0587133 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="223"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9674188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="796" mass="91975" checksum="C073624C8028CE30" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YQ71</accession>
    <name>A0AA38YQ71_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_023848</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YQ71"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9674552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="166" mass="16693" checksum="08104F388CD5B426" modified="2024-01-24" version="1">MEIGVEVEGIGDLVVVESGVEVEGSGRAVVVEIGVEVEGIGDLVVVESGVEVEGSGRAVVAEIGVEVEGSGRAVVVEIGVEVEGIGDLVVVESGVEVEGSGGVVGVESGAKLVVVGGTAVDGQKVVAWNVVLDGAKILEGDASLELEIEHWDRGMALVLQSVQKVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YTA4</accession>
    <name>A0AA38YTA4_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_024858</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the plant Proton pump-interactor protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9676064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YTA4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055282">
        <property type="entry name" value="PPI1-4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32219:SF3">
        <property type="entry name" value="CALPONIN-LIKE DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32219">
        <property type="entry name" value="RNA-BINDING PROTEIN YLMH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="20"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9676064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="117" mass="12946" checksum="DED6A62D91FB4F16" modified="2024-01-24" version="1">MVPAEAERADNKLVRKVVNQKNQTAKTKKPMKHASEAVPAPVSGRGEIEGTEEKEHQQTKKEEELARKVEELRSEEEAAMLKEQSLLEEKANVKEALESKRGTAEKANCSVSAATFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YTL9</accession>
    <name>A0AA38YTL9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_025026</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9676302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YTL9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14011">
        <property type="entry name" value="PK_SCY1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051177">
        <property type="entry name" value="CIK-Related_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12984:SF6">
        <property type="entry name" value="SCY1-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12984">
        <property type="entry name" value="SCY1-RELATED S/T PROTEIN KINASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="39"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="735"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="823"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="878"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="746"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="823"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="837"/>
            <end position="846"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9676302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="931" mass="101076" checksum="7A74427E2A1E3026" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YVH6</accession>
    <name>A0AA38YVH6_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_022441</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="7">Can form complexes with MYC2, MYC3 or MYC4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YVH6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080090">
        <property type="term" value="P:regulation of primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000394">
        <property type="entry name" value="MYB transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000001">
        <property type="entry name" value="MYB-related transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015495">
        <property type="entry name" value="Myb_TF_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10641">
        <property type="entry name" value="MYB FAMILY TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10641:SF586">
        <property type="entry name" value="TRANSCRIPTION FACTOR MYB16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="9"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="9"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="62"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="62"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00062314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9677451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="331" mass="36952" checksum="D86184BC6DBE430B" modified="2024-01-24" version="1">MGGSPCCDTTALKRGPWTPEEDRKLLAYIQEHGHGSWRCLPENAGLQRCGKSCRLRWTNYLRPDIKRGKFSLQEEQTIIQLHALLGNRWSAIATHLRNRTDNEIKNYWNTHLKKRLARMGIDPVTHKPSHAALSSPNGDSKNAANLSHMAQWESARLEAEARLVKESKHRQTQHASASAPAPAQLLNKMATRLTPPRCLDVLNSWENLGSKLKIGSSGSNRDIPSPTSTLSFLENVSTISRVESSEITGILGTFGTEIDAFGTQQCDINMTWTMESTDENVNFVHLLLSRSGDGILTDNGGRDGEELEDNKNYWNDITNLMTCHPSRSPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YWF3</accession>
    <name>A0AA38YWF3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="1">2.3.2.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_022582</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="1"/>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YWF3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23797">
        <property type="entry name" value="UBCc_UBE2H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000024">
        <property type="entry name" value="Ubiquitin-conjugating enzyme 5, E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="10">
        <location>
            <begin position="3"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="136"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="142"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="152"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="7">
        <location>
            <position position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00056190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9677684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="169" mass="19305" checksum="7CD47D95DCFE8CCC" modified="2024-01-24" version="1">MMSDYKVETINDGLNEFNVEFHGPKESLYEGGVWKIRVELPDAYPYKSPSIGFVNKIFHPNVDELSGSVCLDVINQSWSPMFDLLNVFEVFLPQLLLYPNASDPLNGDAASMMMKNKDQYEKKVKEYCERYAKKENITNERLEEDSEDEDFSDGKSESSDEDIAGHADP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YWT0</accession>
    <name>A0AA38YWT0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DYW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_022820</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. PCMP-H subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9678050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YWT0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000242">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000427">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032867">
        <property type="entry name" value="DYW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF461">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14432">
        <property type="entry name" value="DYW_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="71"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="172"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="273"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="DYW" evidence="4">
        <location>
            <begin position="468"/>
            <end position="560"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9678050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="560" mass="62843" checksum="41FDEB837FA77ACF" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YX12</accession>
    <name>A0AA38YX12_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_023008</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9678273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YX12"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="58"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9678273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="151" mass="17807" checksum="BAB00810D22EAD85" modified="2024-01-24" version="1">MKKAMMATWSESEESSEKEKEKEVANMCFMAIDDLDELNSNFSDEDMHNIFEELYEDFEKISLKNNSLKKKIQELEKELEEVKERFSIVEISKTHLEKENEILKKRNEWLTSSLSIFSCGQKSFEMILASQKCVFDKQGLGFKSSKNQKYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YXE3</accession>
    <name>A0AA38YXE3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_020592</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YXE3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010158">
        <property type="term" value="P:abaxial cell fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000002">
        <property type="entry name" value="Myb family transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044847">
        <property type="entry name" value="KAN_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006447">
        <property type="entry name" value="Myb_dom_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01557">
        <property type="entry name" value="myb_SHAQKYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31496">
        <property type="entry name" value="TRANSCRIPTION FACTOR KAN2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31496:SF25">
        <property type="entry name" value="TRANSCRIPTION FACTOR KAN3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0221">Differentiation</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="168"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="288"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="10"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="288"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9678450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="340" mass="38381" checksum="2231DCF482C5E082" modified="2024-01-24" version="1">MELFPAQPDLSLQISPPNAKPTSSWSRRDHQEADLGFWRSSMDSNKATPSRKPDAASDLSLANPKALEPSSNHIHPLHPKYHTLLAPPQLQQKYHHHQEGLHQQIGFLRPIRGIPIYQSTPPSFSFPHQHSLDSSPSTISSTCFNSNGLQRSRFISRFPTKRSMRAPRMRWTTTLHARFVHAVELLGGHERATPKSVLELMDVKDLTLAHVKSHLQMYRTIKTTDRPPASSGQLDGLEGRLAGELSEDNMVDIPKNPQRCELVHQSRLGFDQDLDSCSLWKNSSRDDAWLHGKPRDSRGSTTPFEDTASHERVVMDVNSTSFSRMSPKKPNLEFTLGRSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YY39</accession>
    <name>A0AA38YY39_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_020843</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YY39"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22155">
        <property type="entry name" value="F-box_AtADLO1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46976:SF2">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46976">
        <property type="entry name" value="PROTEIN ARABIDILLO 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="F-box" evidence="3">
        <location>
            <begin position="32"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="2">
        <location>
            <begin position="424"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="2">
        <location>
            <begin position="465"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="2">
        <location>
            <begin position="552"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="2">
        <location>
            <begin position="593"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="2">
        <location>
            <begin position="637"/>
            <end position="679"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9678769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="870" mass="93668" checksum="021A00B6D16F086A" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YYQ3</accession>
    <name>A0AA38YYQ3_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_021036</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YYQ3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000406">
        <property type="entry name" value="Leucine-rich repeat (LRR) family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000041">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000111">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001347">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001646">
        <property type="entry name" value="Putative inactive leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046956">
        <property type="entry name" value="RLP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063">
        <property type="entry name" value="LRR RECEPTOR-LIKE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063:SF16">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041255590" evidence="13">
        <location>
            <begin position="27"/>
            <end position="1036"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="986"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="14">
        <location>
            <begin position="35"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="15">
        <location>
            <begin position="112"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="15">
        <location>
            <begin position="396"/>
            <end position="543"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9678992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1036" mass="113590" checksum="ADDF1635D748E328" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z0T6</accession>
    <name>A0AA38Z0T6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">NB-ARC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_019401</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z0T6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056789">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766:SF40">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766">
        <property type="entry name" value="PLANT BROAD-SPECTRUM MILDEW RESISTANCE PROTEIN RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25019">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NB-ARC" evidence="5">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="6">
        <location>
            <begin position="236"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="R13L1/DRL21-like LRR repeat region" evidence="7">
        <location>
            <begin position="500"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="938"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="938"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="951"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="971"/>
            <end position="984"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF25019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9680102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="984" mass="111842" checksum="9DE1652863F2B37D" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z179</accession>
    <name>A0AA38Z179_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_019504</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z179"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9680218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="81" mass="8999" checksum="EEAC71FB7D04432E" modified="2024-01-24" version="1">MKRSDAVPNKFTYPSLIKACSKVCAVKEGVTFHGLAMRCGVGGDVFVMMSLIDLYGKCGRFCVHTRCLMKCVRGMWFLGQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z1A2</accession>
    <name>A0AA38Z1A2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Acyl-activating enzyme 19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_019556</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z1A2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043041">
        <property type="term" value="P:amino acid activation for nonribosomal peptide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05930">
        <property type="entry name" value="A_NRPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000012">
        <property type="entry name" value="D-alanine--D-alanyl carrier protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000883">
        <property type="entry name" value="Putative acyl-activating enzyme 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052091">
        <property type="entry name" value="Beta-ala_Activ/Resist"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_b-propeller_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002372">
        <property type="entry name" value="PQQ_rpt_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44394">
        <property type="entry name" value="BETA-ALANINE-ACTIVATING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44394:SF1">
        <property type="entry name" value="BETA-ALANINE-ACTIVATING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13570">
        <property type="entry name" value="Beta-prop_ACSF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="4">
        <location>
            <begin position="140"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="5">
        <location>
            <begin position="530"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="domain" description="Pyrrolo-quinoline quinone repeat" evidence="6">
        <location>
            <begin position="822"/>
            <end position="1140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9680277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1149" mass="127495" checksum="BA870CA6ECC4A0FD" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z5F6</accession>
    <name>A0AA38Z5F6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Disease resistance R13L4/SHOC-2-like LRR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_018479</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z5F6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766:SF70">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RGA4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766">
        <property type="entry name" value="PLANT BROAD-SPECTRUM MILDEW RESISTANCE PROTEIN RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0611">Plant defense</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="3">
        <location>
            <begin position="66"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9682562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="383" mass="43615" checksum="68B7E1DBD4E4F057" modified="2024-01-24" version="1">MYDLVHDLAILAAQPECSMLNFDKANEPKEKETISRFLGKLNNIRTISFLNSDVGLKNYTFFITCLLKFKYMRVLGLHNLSSEELPNSILNLKHLKYLDLMGNYNIKKLPNSIWKLHQLQILHLGQCDFPHKEREVGILCFNSLMILCIMGCHLESLMEGMQSFTALRTLMIVKSPCLSSLPRHLLALKNLIITYCESLDLGNRNGDREDNIQGFGSLRNFFISRLPKLEILPMWILQCENFRALLKEEESLSLTSLNALGMGHLTGLTQLKIKDCLQLSSFLEKMFHFPALTHLTIENYPALAEGCELGIGEDWNKIAHLQDIYLDGERIGSTLHNQVTKSGLDEAATRTSVVHQLYFISLFVGNWNAYLVVGLWSSFDSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z8V1</accession>
    <name>A0AA38Z8V1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_016585</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z8V1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007750">
        <property type="entry name" value="DUF674"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33103">
        <property type="entry name" value="OS01G0153900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33103:SF19">
        <property type="entry name" value="OS09G0544700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05056">
        <property type="entry name" value="DUF674"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9684163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="96" mass="10303" checksum="834CBDC48C1446ED" modified="2024-01-24" version="1">MASEANYIFSDTEKAEEGYVKGAVTYMVTDVLVVKPLSTMSIVDLLNKSNINEVGGELEEKGVDSTMREGLKLLINASLQSKTALTNVFLGNTGQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZDP1</accession>
    <name>A0AA38ZDP1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_015817</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZDP1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044659">
        <property type="entry name" value="PELPK1_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33088">
        <property type="entry name" value="MUCIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33088:SF28">
        <property type="entry name" value="PROTEIN PELPK1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041295076" evidence="2">
        <location>
            <begin position="29"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="113"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9687111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="135" mass="14762" checksum="635E00A432EDB999" modified="2024-01-24" version="1">MARHQEPSILFPLLLITLSLMSGKEVLASRHLLETTLPTVPELPKVELPPLPTLPTLPKFELPPLPKVELPPLPHVPTLQEPQLPTLPKPELPTVPHVPALEKPELPKPTLPTIPTLPKDIPFPSLSPPHSTTSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZF27</accession>
    <name>A0AA38ZF27_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_016243</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZF27"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15568">
        <property type="entry name" value="PHD5_NSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10567">
        <property type="entry name" value="SWIB-MDM2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.200">
        <property type="entry name" value="Plus-3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.245.10">
        <property type="entry name" value="SWIB/MDM2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045894">
        <property type="entry name" value="At5g08430-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035445">
        <property type="entry name" value="GYF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058668">
        <property type="entry name" value="NERD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055198">
        <property type="entry name" value="NSD_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004343">
        <property type="entry name" value="Plus-3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036128">
        <property type="entry name" value="Plus3-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036885">
        <property type="entry name" value="SWIB_MDM2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003121">
        <property type="entry name" value="SWIB_MDM2_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46851">
        <property type="entry name" value="OS01G0884500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46851:SF23">
        <property type="entry name" value="SWIB_MDM2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25980">
        <property type="entry name" value="NERD_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22908">
        <property type="entry name" value="PHD_NSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03126">
        <property type="entry name" value="Plus-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02201">
        <property type="entry name" value="SWIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00719">
        <property type="entry name" value="Plus3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55277">
        <property type="entry name" value="GYF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159042">
        <property type="entry name" value="Plus3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47592">
        <property type="entry name" value="SWIB/MDM2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51360">
        <property type="entry name" value="PLUS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51925">
        <property type="entry name" value="SWIB_MDM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DM2" evidence="6">
        <location>
            <begin position="222"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="Plus3" evidence="5">
        <location>
            <begin position="354"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="542"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="729"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="557"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="626"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="674"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="730"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="741"/>
            <end position="751"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9687679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="825" mass="93222" checksum="B40C20334500FF37" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZH10</accession>
    <name>A0AA38ZH10_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glucan endo-1,3-beta-D-glucosidase</fullName>
            <ecNumber evidence="3">3.2.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_014372</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;3)-beta-D-glucosidic linkages in (1-&gt;3)-beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.39"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the glycosyl hydrolase 17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.39" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZH10"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000005">
        <property type="entry name" value="Glucan endo-1,3-beta-glucosidase 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000490">
        <property type="entry name" value="Glyco_hydro_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044965">
        <property type="entry name" value="Glyco_hydro_17_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32227">
        <property type="entry name" value="GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00332">
        <property type="entry name" value="Glyco_hydro_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="377"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9688685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="398" mass="44394" checksum="3A10A46D35A8C8ED" modified="2024-01-24" version="1">MEWEMKSWRFFYRFLRVFFLVFAMNGSLTVQAFTGTYGINYGRIADNIPSPDEVVTLLRAAKIKNVRIYDADHSVLEAFSGTGLEIVVGVPNGNLKDMNASEDHAESWVKENVQSFLPDTHIRGIAVGNEVLGGDQELWGVLLGAVKNVHKALEKFHLTDLIQVSTAHSQAVFSNSYPPSSCIFNQNIVQYMKPLLEFFSEIHSPFCLNAYPFLDYMGDPANIDINYALFQSTQGIYDTKAKLHYDNMLDAQIDAAYAALENSGFKKMEVIVTETGWASRGDENEAAATSTNARTYNYNLRKRLAKKKGTPLRPKNVVKAYIFAVFNENLKPGPTSERNFGLFKADGSIAYDIGFKGLKSSATSSILSLKKIRAQDWSASFSLVFTACAAVLLLFLRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZH71</accession>
    <name>A0AA38ZH71_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PPPDE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_014303</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DeSI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZH71"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0101005">
        <property type="term" value="F:deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1720.30">
        <property type="entry name" value="PPPDE domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008580">
        <property type="entry name" value="PPPDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042266">
        <property type="entry name" value="PPPDE_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12378">
        <property type="entry name" value="DESUMOYLATING ISOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12378:SF80">
        <property type="entry name" value="IP06716P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05903">
        <property type="entry name" value="Peptidase_C97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01179">
        <property type="entry name" value="DUF862"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51858">
        <property type="entry name" value="PPPDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPPDE" evidence="5">
        <location>
            <begin position="15"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="166"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9688587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="215" mass="23982" checksum="5CEA709A9900F83F" modified="2024-01-24" version="1">MGAANTSISGSEYETQVILNVYDLTPLNNYIYWVGCGIFHSGIEVHDKEYGFGAHDFPASGVFEVEPRSCPGFIYRNSIILGRIKMPPSEFRSFIENVAAEYHGDTYHLISKNCNHFTDDICWRLTGKRIPGWVNRLARLGALCSCLLPESLQVTTVKQLPEYHNYSGKEEGSESVSTATPHDPTESDDADQDKLHLISQSGGSEELSFVKEAQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZIK7</accession>
    <name>A0AA38ZIK7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Shikimate O-hydroxycinnamoyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_014594</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9689023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZIK7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000015">
        <property type="entry name" value="Spermidine hydroxycinnamoyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000008">
        <property type="entry name" value="Tryptamine hydroxycinnamoyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050317">
        <property type="entry name" value="Plant_Fungal_Acyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31642:SF11">
        <property type="entry name" value="SHIKIMATE O-HYDROXYCINNAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31642">
        <property type="entry name" value="TRICHOTHECENE 3-O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="195"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9689023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="433" mass="47960" checksum="D5F7D074A1E77E22" modified="2024-01-24" version="1">MKIEVKKWSMVCPAKHTPKHTLWMSNLDLVVPRIHVPTVYLYKPNAFCSNFFDAGRLKEALSDALVQFYPVAGRLGLDANGRIQINCNGEGVLFQEAEADAIIDDLGDFTPSHQLRHLIPVVDHSADISSYPLLLLQVTYFKCGGVCLGVAIHHMVADGSGCIHFINSWSDITRGIPIAISPFFDRTILRARDPPAPRFSHVEHHPPPPLHGPSTPKPTSTLILHLTPEHLNALKAKSVRHGSPSPSSVKYSTYETLAAHLWRCICKARGLPHDQPTKLYIPTDGRLRLRPTLPPGYFGNALFTSTLTAKSGDLQSEALSDTIQRIHNAIAGMEDEYLRSAVDYLEMQPNLTALVRGAHTFRSPNLVVGSWTRLPINDANFGWGRPMHMGLGSGVFEGNVCIQRSPTKDNHLSVVICLEAQPMQLFKDLLYDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZJ12</accession>
    <name>A0AA38ZJ12_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Factor of DNA methylation 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_014786</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9689278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZJ12"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080188">
        <property type="term" value="P:gene silencing by siRNA-directed DNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12266">
        <property type="entry name" value="RRM_like_XS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2890">
        <property type="entry name" value="XS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045177">
        <property type="entry name" value="FDM1-5/IDN2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005379">
        <property type="entry name" value="FDM1-5/IDN2_XH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005380">
        <property type="entry name" value="XS_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038588">
        <property type="entry name" value="XS_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005381">
        <property type="entry name" value="Znf-XS_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21596:SF3">
        <property type="entry name" value="FACTOR OF DNA METHYLATION 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21596">
        <property type="entry name" value="RIBONUCLEASE P SUBUNIT P38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03469">
        <property type="entry name" value="XH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03468">
        <property type="entry name" value="XS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03470">
        <property type="entry name" value="zf-XS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0943">RNA-mediated gene silencing</keyword>
    <feature type="domain" description="Zinc finger-XS" evidence="6">
        <location>
            <begin position="43"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="XS" evidence="4">
        <location>
            <begin position="115"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="Factor of DNA methylation 1-5/IDN2" evidence="5">
        <location>
            <begin position="502"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="348"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="348"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9689278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="633" mass="73876" checksum="F05CBC8BB90E7BC9" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZJV8</accession>
    <name>A0AA38ZJV8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Apple domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_012709</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZJV8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048544">
        <property type="term" value="P:recognition of pollen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01098">
        <property type="entry name" value="PAN_AP_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000858">
        <property type="entry name" value="S_locus_glycoprot_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32444">
        <property type="entry name" value="BULB-TYPE LECTIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32444:SF63">
        <property type="entry name" value="G-TYPE LECTIN S-RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE RKS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08276">
        <property type="entry name" value="PAN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00954">
        <property type="entry name" value="S_locus_glycop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041332181" description="Apple domain-containing protein" evidence="4">
        <location>
            <begin position="19"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="5">
        <location>
            <begin position="245"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9690185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="326" mass="37044" checksum="CBB5F4DF9E3EF181" modified="2024-01-24" version="1">MTWLKLLQFCSSVVHCLSLHIPSVNATVTQLLDPGNLVLIQNDDKRVVWQGFDYPTHHLIPYMKLGLDRRTDFNRFLTSWKFPTDPGTGKNSLRKYTSGSAQIFLYQGSEPLWRSGNWNRLRWSGLPVMMHRTIINASFLNNQDEISYMFTVVNAPVFSRMTVDLDDYLQRYTWQETKGKWFSFYTAPRDRCDKYSRCGPNSNWDNSLAEFECTCLVGFEPKSPRDWFLKDGSAGRLRKEGAKVCGNGEGFVKVEDAKPPDTTVARVNMNISMEACREECRKECSCSGYAAANVSGSGSGCLSWHGGWGRKDLCVCGCHHSRYASI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZK60</accession>
    <name>A0AA38ZK60_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein DETOXIFICATION</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Multidrug and toxic compound extrusion protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_012442</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9689759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZK60"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13132">
        <property type="entry name" value="MATE_eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045069">
        <property type="entry name" value="MATE_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="138"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="167"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="200"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="229"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="283"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="316"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="351"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="397"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="426"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="457"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9689759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="501" mass="54191" checksum="71715E5F73FE3A01" modified="2024-01-24" version="1">MESTQDELAQPILLPKQYSSSPEPVSSELEEVLSNTDLSYFRRIGSATLIELNILFRLAGPAVVVYLLNNVTSMSTQIFCGHLGNLELAASALGNNGIQIFSYGLMLGMGSAVETLCGQAYGAHKFDMLGVYLQRSTILLTATGIPLMIIYIFSKDLLLLLGESTEIASAAAIFVYGLIPQIFAYATNFPIQKFLQAQSIVFPSAYISAATLCVHLLLSWLAIYKLGLGLLGASLILSLSWWIIVVAQFVYILVSDRCKYTWTGFSLQAFSGLWEFLKLSTASAVMLCLETWYYQILVLIAGLLENAEIALDSLSICMTISGWVFMIAVGFNAAASVRVSNELGAGHPKSASFAVIVVTSTSFIIAVFLAILVMLLRHVMSYVFTGGTTVADAVSDLAPFLAVSIVLNGIQPVLSGVAVGCGWQAFVAYVNVGCYYVVGIPLGSLLGFKFDLGVKGIWSGMIGGTVMQTLILLWVTYRTDWIKEVSINAWDLNGFQALMLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZLZ7</accession>
    <name>A0AA38ZLZ7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_013663</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZLZ7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044822">
        <property type="entry name" value="Myb_DNA-bind_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33492:SF12">
        <property type="entry name" value="HOMEODOMAIN-LIKE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33492">
        <property type="entry name" value="OSJNBA0043A12.37 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13837">
        <property type="entry name" value="Myb_DNA-bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="13"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="211"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="217"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9691551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="301" mass="33414" checksum="94591EEA45F167F4" modified="2024-01-24" version="1">MADQGGSSSVAVMRDYRKGNWTVNETMILIEAKKMDDQRRMKRSGENEGRSKPAELRWKWVEDYCWRKGCLRSQNQCNDKWDNLMRDYKKVRDYERRLLSERGDSGDHDGGSSSSSSYWRIDKNERKEKNLPSNMLRQIYEGLVEVVERKGVSTTSSATAAAAAAAAAAHQRVMSGGGGGLGLGSIPNIGYVVDRIEPPLPPLLQLHNPSPVSVAALPPPPPPLPPPPSAPQPPQQPQQPHIAYPQPLPSLAQILIQVSIQTHQQRGGEEVVEKAQVVVLLLQVEAPQLKWVLQFQKVLPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZM33</accession>
    <name>A0AA38ZM33_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Fungal lipase-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_013330</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZM33"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047714">
        <property type="term" value="F:galactolipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008970">
        <property type="term" value="F:phospholipase A1 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00519">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000065">
        <property type="entry name" value="Phospholipase A1-II 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002921">
        <property type="entry name" value="Fungal_lipase-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31403:SF11">
        <property type="entry name" value="OS12G0614500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31403">
        <property type="entry name" value="PHOSPHOLIPASE A1-IBETA2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01764">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4" id="KW-0934">Plastid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Fungal lipase-type" evidence="9">
        <location>
            <begin position="216"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9691107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="510" mass="58030" checksum="48DFE84086E108AD" modified="2024-01-24" version="1">MKAISSSCIHHHLPVAHHRRNNQTFIIRGFQEVATLPKQQNASSRLSQSLSNLLRLHVDTPPRTDFQSSTGISLIDEKHSTPTCSPKEDISTKWGEIHGSCDWDNLLDPLQPWLRREIVKYGEFAQATYDAFDFDSFSEYCGSCRYNQHKLFKELGLDKNGYMVSKYIYAMSHIDVPRWLERSHLLDTWSKDSNWMGYVAVSDDQESRRIGRRDIVVAWRGTVAPSEWYEDFQRKLEPVGPGEAKVEHGFLSLYTSKRESSRYNKSSASDQVMKEVTRLVQLYKQRGEQVSLTITGHSLGGALALLNAYEAATSLPGLPISVISFGAPRVGNIAFRDELHQLGVKTLRVVVKQDIVPRMPGLVFNESLQKFDDITGTLKWVYTHVGAELKLDVQSSPYLKRGFNLPGFHSLETYLHLIDGFHSKTSTFREDARRDVALVNKACDMLVDELRIPHAWYQFENKGLVRNSHGRWVKQVRDPEDIPSPARAAHHQAFSVEMLETDEGLALTLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZN53</accession>
    <name>A0AA38ZN53_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">PVL29_011305</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="25">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the leguminous lectin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="25">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002229">
        <property type="term" value="P:defense response to oomycetes"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000240">
        <property type="entry name" value="Lectin-domain containing receptor kinase A4.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000129">
        <property type="entry name" value="Leucine-rich repeat receptor-like kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000394">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000146">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase IOS1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024788">
        <property type="entry name" value="Malectin-like_Carb-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45631">
        <property type="entry name" value="OS07G0107800 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45631:SF206">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12819">
        <property type="entry name" value="Malectin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 22" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="13 22" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Protein kinase" evidence="24">
        <location>
            <begin position="498"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="24">
        <location>
            <begin position="1136"/>
            <end position="1410"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="24">
        <location>
            <begin position="1517"/>
            <end position="1816"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="1449"/>
            <end position="1469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="1451"/>
            <end position="1460"/>
        </location>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="1165"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="KAJ9692166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1816" mass="200945" checksum="9434B06ADA5B6620" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZST7</accession>
    <name>A0AA38ZST7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Probable DNA helicase MCM8</fullName>
            <ecNumber evidence="3">3.6.4.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Minichromosome maintenance 8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PVL29_010224</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the MCM family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZST7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17759">
        <property type="entry name" value="MCM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22247">
        <property type="entry name" value="MCM8_WHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.10:FF:000007">
        <property type="entry name" value="DNA helicase MCM8 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056875">
        <property type="entry name" value="MCM8/REC_WHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF47">
        <property type="entry name" value="DNA HELICASE MCM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25051">
        <property type="entry name" value="WHD_MCM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9 16" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0469">Meiosis</keyword>
    <keyword evidence="4 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="17">
        <location>
            <begin position="342"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00042306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00069556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU004070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ9694653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="783" mass="86742" checksum="BCF1C6A897F205B7" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZTM0</accession>
    <name>A0AA38ZTM0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_010525</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZTM0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.330:FF:000005">
        <property type="entry name" value="1-aminocyclopropane-1-carboxylate oxidase homolog 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10209:SF546">
        <property type="entry name" value="1-AMINOCYCLOPROPANE-1-CARBOXYLATE OXIDASE HOMOLOG 4-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10209">
        <property type="entry name" value="OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="7">
        <location>
            <begin position="208"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9695070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="363" mass="40873" checksum="E03485240CC36989" modified="2024-01-24" version="1">MSSADYDRMKEVKEFAESKIGVKGLSDSGIASIPRIFIHPPQTLSQLKSTSSSSFSSIHIPVIDLSNLDSPHHRPKIVDQIREATKTWGFFQVINHGVALSVLEETINAVKSFHDQPHQVKAKLSVGEERRGVVYTSNNDLYRSEAATWHDYLQVWMAPQPPEVERIPEICRREVIAWDTHAKNVAETLMELLSEGLGLEPGKFKELTFSDTRLFAGVCYPHCPQPDLTMGLASHTDPTVLTVLLQNHVPGLQVKHGDEWVDVKPVPGGLIINAGDFLQIVSNGEYNSIQHRVIANPCKEPRLSLVMFFNLAKWKESDSYGPLPELLSPEKPAIYRNFTRQEYDDNFYSKGLDSKSFIDKIRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZUM5</accession>
    <name>A0AA38ZUM5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_010545</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZUM5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47941">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9695097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="417" mass="47743" checksum="058F94A2BB87836F" modified="2024-01-24" version="1">MVSNPCPCSDGVFSCPKSEPIFRFALNAFCKVGILVDAEIAFANMGRMIDGKLVRREHEKVMAVYEKMIKDRVKPDVFIFNTLIRSSRNSLFGLALKMFKEMKDKGRSPNVFSFHTLIKGSFRERKFEEGTGMAYEVLELGCQFSNGKEHYLMGLIVFVSLRRFVVKKMWGGHWSLWTAMEERKCTKLDCLYYFDRRIEEIRKNRRSISINGKDGKREGILPCRVTYNCLLQDLCEVGRTCEANILRLLASASPRWFHGFHFALRTTTLGSSLGLHSSAHSASAFAAPTCVLVAPCHTNPNIWTKFGFLHLQHTGYNNKGLPHDIFWFLENRRKGKKNKARELTLVCFDDMENDVEKEMKEKSQASVRLPLSFHQPASSLHQWLPLPRSLLPSTIIISATFQCFGKSTPAWLTVFDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZUN8</accession>
    <name>A0AA38ZUN8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Eukaryotic translation initiation factor NCBP</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Novel cap-binding protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">mRNA cap-binding protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_010896</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">EIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G. EIF4E is also known to interact with other partners. In higher plants two isoforms of EIF4F have been identified, named isoform EIF4F and isoform EIF(iso)4F. Isoform EIF4F has subunits p220 and p26, whereas isoform EIF(iso)4F has subunits p82 and p28.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the eukaryotic initiation factor 4E family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZUN8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016281">
        <property type="term" value="C:eukaryotic translation initiation factor 4F complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000340">
        <property type="term" value="F:RNA 7-methylguanosine cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009615">
        <property type="term" value="P:response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.760.10:FF:000008">
        <property type="entry name" value="Eukaryotic translation initiation factor NCBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.760.10">
        <property type="entry name" value="RNA Cap, Translation Initiation Factor Eif4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023398">
        <property type="entry name" value="TIF_eIF4e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001040">
        <property type="entry name" value="TIF_eIF_4E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019770">
        <property type="entry name" value="TIF_eIF_4E_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960:SF18">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E HOMOLOGOUS PROTEIN, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01652">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55418">
        <property type="entry name" value="eIF4e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00813">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="6 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0810">Translation regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="14"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="27"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00074446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00078952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9695640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="225" mass="25905" checksum="96712993B0CB6331" modified="2024-01-24" version="1">MEITEKKEAESKGLNPQSVTEATSFNSDKEAEDRERQARDLKAGLHPLKHKFVFWYTRRTPGVRTQTSYEDNIKKIVDFSTVEGFWICYCHLARPSALPSPTDLHLFKEGIRPLWEDSANCNGGKWIIRFKKVVSGRFWEDLVLALVGDQLDYGDNICGAVLSIRFNEDILSVWNRNASDHQAVMALRDAIKRNLKLPHGYVMEYKAHDASLRDNSSYRNTWLRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZWA1</accession>
    <name>A0AA38ZWA1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_010815</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZWA1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9695519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="62" mass="6951" checksum="2E40B0C79480B688" modified="2024-01-24" version="1">MVPEDVPFGESPISPDWGIINVLSGHMIDPTSCCFDGNLEEHAGRYYDSSRRPRVVFQGDPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZWW7</accession>
    <name>A0AA38ZWW7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pectin acetylesterase</fullName>
            <ecNumber evidence="6">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_008825</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 6">Secreted</location>
            <location evidence="2 6">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the pectinacetylesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="6"/>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZWW7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009505">
        <property type="term" value="C:plant-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052793">
        <property type="term" value="F:pectin acetylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004963">
        <property type="entry name" value="PAE/NOTUM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21562">
        <property type="entry name" value="NOTUM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21562:SF98">
        <property type="entry name" value="PECTIN ACETYLESTERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03283">
        <property type="entry name" value="PAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0134">Cell wall</keyword>
    <keyword evidence="5 6" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041482564" description="Pectin acetylesterase" evidence="6">
        <location>
            <begin position="25"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9696810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="408" mass="44740" checksum="DD722563191BB74F" modified="2024-01-24" version="1">MSVMAVGVVAVAWCLFFVNGHVLGEDGFQGLLDLEDIPSPNPRMVGLTLIPGAAAKGAVCLDGTLPGYHFQPGFGSGANSWLIQLEGGGWCNNIKSCVFRKTTLRGSSKYMEKTLAFTGILSNKAEENPDFFNWNRVKLRYCDGASFSGEGQNKATGLYFRGQRIFEAGMEELMSKGMKNADQALLSGCSAGGLASILHCDEFRGLFPKITKVKCLSDAGLFLDATDAAGGHTIRNLYAGVVSLQGVEKNLPSTCTTRLDPTSCFFPENLINNITTPLFLLNAAYDAWQFQESLVPSSVDPHGEWKACKSNQSNCNSTQIQFLQDFRMEMLDDVKGFASYDQNGLFINSCFAHCQTLRQDTWFADDSPLIGKKKIAESVGDWYFDRRPVKAIDCPYPCDSTCHNLVFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZX56</accession>
    <name>A0AA38ZX56_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_008281</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9695939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZX56"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11064">
        <property type="entry name" value="CYP86A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24296">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0349">Heme</keyword>
    <keyword evidence="6 8" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="8">
        <location>
            <position position="449"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9695939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="507" mass="57749" checksum="68599D9E44F264DD" modified="2024-01-24" version="1">MFEFSLLLSPFLFILPLLFFFFFSFIRTKSSSSPSASALKSYPIIGSYLEIFANLNRRVQWTSDLIRASPSATVVIHRCLGIRVVLTGNHSNVQHILKTHFHNYHKGQSFTINLSDFLGGGIFNADGDNWKFQRQISSHEFNTKSLRKFIETVVDAELSDRLIPILSTAAANNSVLDFQDILQRFAFDNICKIAFGYDPAYLLPSLPKAKFAVAFEDAARISSERFTSILPIVWKLKRLMGVGSEKRLKLAVAEVREFAKNIVREKKEELKHKSSLDSTDLLSRFLSSGHSDEDFVTDIVISFILAGRDTTSAALTWFFWLLFKHPDVEDQILNEINEKSELPVFDEVKDMVYTHASLCESMRFYPPVPVDSKEVVSDDVLPDGTVVRKGMRVIYHPYAMGRLEELWGSDWPEFRPERWLQRDPDTGKWSFVARDPYTYPVFQAGPRICLGKEMAFLQMKRVVAGVLRQFQVVPVVEDGGEPVFTADLAAKMKGGFPVRIVERADRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZXT6</accession>
    <name>A0AA38ZXT6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">LOB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_008891</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LOB domain-containing protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZXT6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009755">
        <property type="term" value="P:hormone-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004883">
        <property type="entry name" value="LOB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31529">
        <property type="entry name" value="LOB DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31529:SF23">
        <property type="entry name" value="LOB DOMAIN-CONTAINING PROTEIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03195">
        <property type="entry name" value="LOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50891">
        <property type="entry name" value="LOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LOB" evidence="3">
        <location>
            <begin position="7"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="88"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9696890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="195" mass="21478" checksum="33CD6E7E21273A3C" modified="2024-01-24" version="1">MASGSGSPCGACKFLRRKCASDCVFAPYFCSEQGPARFAAIHKVFGASNVSKLLLHVPVADRCEAVVTIAYEAQARIRDPVYGCVAHIFALQQQVACLQAQLMQLKAQLANNMGNLENQWQGNVSGAPSFQNFPAYMNSVSPQCSLESIDHNSDGMAMQDIQSRDEFSFQTCLRKRPPHNDLGELQELALRMMRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZXV8</accession>
    <name>A0AA38ZXV8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_008832</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZXV8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010017">
        <property type="term" value="P:red or far-red light signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11445">
        <property type="entry name" value="bHLH_AtPIF_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000004">
        <property type="entry name" value="Basic helix-loop-helix transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047265">
        <property type="entry name" value="PIF1-like_bHLH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044273">
        <property type="entry name" value="PIF3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46807:SF3">
        <property type="entry name" value="BHLH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46807">
        <property type="entry name" value="TRANSCRIPTION FACTOR PIF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="6">
        <location>
            <begin position="386"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="156"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="220"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="325"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="169"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="328"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="348"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="386"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9696822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="574" mass="61840" checksum="C2D3918A44667259" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZXX4</accession>
    <name>A0AA38ZXX4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Basic blue protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Plantacyanin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_009145</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZXX4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11013">
        <property type="entry name" value="Plantacyanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000013">
        <property type="entry name" value="basic blue protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039391">
        <property type="entry name" value="Phytocyanin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003245">
        <property type="entry name" value="Phytocyanin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041844">
        <property type="entry name" value="Plantacyanin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33021">
        <property type="entry name" value="BLUE COPPER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33021:SF469">
        <property type="entry name" value="PHYTOCYANIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02298">
        <property type="entry name" value="Cu_bind_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51485">
        <property type="entry name" value="PHYTOCYANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0186">Copper</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041241385" description="Basic blue protein" evidence="6">
        <location>
            <begin position="33"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="Phytocyanin" evidence="7">
        <location>
            <begin position="33"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00071970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00082491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9697232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="129" mass="13835" checksum="EA13D9D70007A525" modified="2024-01-24" version="1">MALQGRCSANHAIALTAILLVFVLLHVKASQATTFMVGDSSGWTFNMNNWASGKKFKAGDKLVFKYNPSFHNVVATDEDGYNGCSTASPSSKVYSTGNDAVKLLKGHNYFICGVPGHCDMGLKIRVNAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZZE0</accession>
    <name>A0AA38ZZE0_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_007295</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZZE0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36721:SF15">
        <property type="entry name" value="EN_SPM-LIKE TRANSPOSON PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36721">
        <property type="entry name" value="PROLINE-RICH FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041470336" evidence="3">
        <location>
            <begin position="24"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="127"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="35"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9698130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="168" mass="17123" checksum="D03461288DBA0B3D" modified="2024-01-24" version="1">MARLFRFCIALTAVLLLVEFAVAVDPPELSPAPAPASGAEDVAPTPHTLSPPLSPAAPSPSPYFGSPPAPPLASESSPSPSPDSSPSPTPSPTPSSSDVGDIQHTSTENEDGEEGESSGGLKGGQKAGIALGVIACVAVLGFGCLVYKKRQDNIQRSRYGYAARTEIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZZG5</accession>
    <name>A0AA38ZZG5_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_007236</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZZG5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009515">
        <property type="entry name" value="DUF1138"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34267:SF17">
        <property type="entry name" value="OS06G0114500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34267">
        <property type="entry name" value="OS11G0161033 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06592">
        <property type="entry name" value="DUF1138"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9698027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="81" mass="8931" checksum="261BF4DF2639BD6A" modified="2024-01-24" version="1">MATKYIIGSVVGAFALAYAFDSAISDRKLFGGSTPKTVKDEVWWEETDKKAKVWPRTAGPPVALNPITRQNYTILKARSEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A183</accession>
    <name>A0AA39A183_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NAB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_007798</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the NET family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A183"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011684">
        <property type="entry name" value="NAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051861">
        <property type="entry name" value="NET_actin-binding_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32258">
        <property type="entry name" value="PROTEIN NETWORKED 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32258:SF3">
        <property type="entry name" value="PROTEIN NETWORKED 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07765">
        <property type="entry name" value="KIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51774">
        <property type="entry name" value="NAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAB" evidence="5">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="61"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="114"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="168"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="227"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="373"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="503"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="123"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="148"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9698899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="574" mass="65971" checksum="982106026067E3E3" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A2S0</accession>
    <name>A0AA39A2S0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">PVL29_005593</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="22">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9699796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A2S0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.430:FF:000003">
        <property type="entry name" value="FERONIA receptor-like kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.430:FF:000007">
        <property type="entry name" value="FERONIA receptor-like kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000058">
        <property type="entry name" value="Receptor-like protein kinase FERONIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000039">
        <property type="entry name" value="receptor-like protein kinase FERONIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.430">
        <property type="entry name" value="Galactose-binding lectin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045272">
        <property type="entry name" value="ANXUR1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024788">
        <property type="entry name" value="Malectin-like_Carb-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34590">
        <property type="entry name" value="OS03G0124300 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34590:SF5">
        <property type="entry name" value="OS04G0586500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12819">
        <property type="entry name" value="Malectin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0278">Fertilization</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="12 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7 20" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041405238" description="non-specific serine/threonine protein kinase" evidence="20">
        <location>
            <begin position="26"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="439"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="527"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="833"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="833"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="555"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAJ9699796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="859" mass="94273" checksum="0B9B8CDE227ACD1C" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A376</accession>
    <name>A0AA39A376_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transcription factor CBF/NF-Y/archaeal histone domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_005728</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NFYB/HAP3 subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A376"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016602">
        <property type="term" value="C:CCAAT-binding factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22907">
        <property type="entry name" value="HFD_NFYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000035">
        <property type="entry name" value="Nuclear transcription factor Y subunit B-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027113">
        <property type="entry name" value="Transc_fact_NFYB/HAP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003956">
        <property type="entry name" value="Transcrpt_fac_NFYB/HAP3_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11064">
        <property type="entry name" value="CCAAT-BINDING TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11064:SF149">
        <property type="entry name" value="OS01G0935100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00615">
        <property type="entry name" value="CCAATSUBUNTA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00685">
        <property type="entry name" value="NFYB_HAP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription factor CBF/NF-Y/archaeal histone" evidence="7">
        <location>
            <begin position="22"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="116"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9700036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="138" mass="15420" checksum="3CD726459C76C3ED" modified="2024-01-24" version="1">MVDTLGASASTDDGSVKEQDRLLPIANVSRIMKQTLPTNAKISKEAKETMQECVSEFISFVTGEASEKCKKERRKTVNGDDVCWALAALGFDDYAGPLKRYLQRYRELEGDRVLNQEKAGNAEENDEPSLYRGSQPRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A7D0</accession>
    <name>A0AA39A7D0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_006966</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A7D0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035770">
        <property type="term" value="C:ribonucleoprotein granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901259">
        <property type="term" value="P:chloroplast rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000963">
        <property type="term" value="P:mitochondrial RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044528">
        <property type="term" value="P:regulation of mitochondrial mRNA stability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.960.10">
        <property type="entry name" value="VSR Endonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050870">
        <property type="entry name" value="FAST_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013584">
        <property type="entry name" value="RAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058917">
        <property type="entry name" value="RESC6_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21228">
        <property type="entry name" value="FAST LEU-RICH DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21228:SF40">
        <property type="entry name" value="LD45607P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08373">
        <property type="entry name" value="RAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26188">
        <property type="entry name" value="RESC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00952">
        <property type="entry name" value="RAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51286">
        <property type="entry name" value="RAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RAP" evidence="2">
        <location>
            <begin position="581"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="140"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="156"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9701817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="656" mass="74055" checksum="C4A29078965F0C5F" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A8B8</accession>
    <name>A0AA39A8B8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_004491</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A8B8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36617">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36617:SF15">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase zinc-binding" evidence="1">
        <location>
            <begin position="124"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9702782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="319" mass="36451" checksum="848C852E434AADC9" modified="2024-01-24" version="1">MVVRWSHRCPWKAIAQVSQNFSPFVLLVVGNGERIRFWEDLWWGNQTLCSQFAGLYRVISVKNLTVSNVLGNSFPLSWNLNFCRNLTDSEIDLLQSLMSSLSSVLFSPSLADSREWSLLSSGLFSVKSFFLALSKVSNPILFLPAKFLWSSKAPSKVKAFAWLVAHGWVNTNDKLQLRRPYKSLCPQWCILCKGNGESIDHLFLYCPVTIGLWHKLFNLAGLTWVPPRSIEEMLVIAFKGLGNSLRGKTLWQIACLTLLWMVWQERNNRIFEEEGRTEEMLWDLILFYSSLWASCTAAFRGVPLSVLQLSWKAVCVSKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AA39</accession>
    <name>A0AA39AA39_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_005124</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AA39"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36347">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36347:SF1">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="139"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="49"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9703696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="180" mass="19904" checksum="61B298FB61B954CD" modified="2024-01-24" version="1">MAMETNLLQIPFLYSKSSSSSSPILSLTKPHLQIFIRSQPHRLRSLTVTPINSSSSSDVPPPVPPPVTPPETVEIRFRRGSKKRRQTQQQDGGVAIKAAETSSSTPPPPLKDWKDMSITEKAIELYMGEKGALFWLNKFAYASIFIVIGAWILFRFVGPALNLYQLDSAPLSPSSMFKGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AAP9</accession>
    <name>A0AA39AAP9_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_004655</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AAP9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000023">
        <property type="entry name" value="protein REVEILLE 6 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006447">
        <property type="entry name" value="Myb_dom_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01557">
        <property type="entry name" value="myb_SHAQKYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12802:SF155">
        <property type="entry name" value="DEUBIQUITINASE MYSM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12802">
        <property type="entry name" value="SWI/SNF COMPLEX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="55"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="7">
        <location>
            <begin position="55"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="8">
        <location>
            <begin position="58"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="113"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="159"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="186"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="279"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="129"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="160"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="186"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="218"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="283"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="309"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9702988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="479" mass="53314" checksum="B6D84A3CDCA895F7" modified="2024-01-24" version="1">MTVEDQSGGSRSNTFLSAGNLISLDAGTHSLTAAELKDQFSCRDDLAPKARKPYTITKQRERWTEEEHKKFLEALKLYGRAWRRIEEHVGTKTAVQIRSHAQKFFSKVVRETTGGNTNSVEPIEIPPPRPKRKPVHPYPRKLVHPLRKGISVLEPPVRSVSPNFSASERENQSPTSVLSAVGSETLGSTLSNTQNGSPSPVSSAAGVNPDSMFLPELNQPTEENASPSPVLTTSSFVPDEPLPMKFELFPKEIFAKECSVEEASTRTLKLFGRTVLVTDSHRPSSPTTSKSQPSDINNEGMHVETLPWNSTPTESSPENTSFSWSHLQPQPSGAFYYMHFQKEDSNCVEAGTTAPLPWWTFYGGIPIPFLPLHHQDSIKAHEDTKFAETHDKELQKEGSWTGSNTGSVNDGGNCDTNWDVETQSHQPCLDTMEKESDTAFELKLSERSAFSRGRTNTDKCVKGFMPYKDVWQRETPSHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ACQ2</accession>
    <name>A0AA39ACQ2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PRISE-like Rossmann-fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_003308</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ACQ2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd08948">
        <property type="entry name" value="5beta-POR_like_SDR_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000808">
        <property type="entry name" value="Iridoid synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055222">
        <property type="entry name" value="PRISE-like_Rossmann-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32487">
        <property type="entry name" value="3-OXO-DELTA(4,5)-STEROID 5-BETA-REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32487:SF0">
        <property type="entry name" value="3-OXO-DELTA(4,5)-STEROID 5-BETA-REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22917">
        <property type="entry name" value="PRISE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRISE-like Rossmann-fold" evidence="3">
        <location>
            <begin position="89"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9705186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="389" mass="44102" checksum="843C27C1596FF2F6" modified="2024-01-24" version="1">MSWWWSGAIGAAKRKLQEDEAHPTNYQGVGLIVGVTGIVGNSLAEILPLRDTPGGPWKVYGIARRSQPAWNADNCVEYIQCDVLDPEETSSKLSKLTDVTHIFYVTWANMGSEAENCRVNGDMFRNVLSAVIPNAPNLQHICLQTGRKHYIGPFEAFGKIEPHDPPYHEEMPRLDVENFYHVQEDILFEEVRKKEGLTWSVHRPGIIFGFSPYSMMNAIGTLCVYATICKYEGLPLRFPGTQDTWNGYWDASDADLIAEHHIWAAVDPFAKNEAFNCSNGDVFKWKHLWKVLAEQFGLEFHEPEGQGLSLEKMMKEKGPVWDEIVREKGLVPTKLEEVGQWWFADVILSAGSTLDSMNKSKEHGFLGFRNSKSSFLSWIDKMKAYKLVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ADM0</accession>
    <name>A0AA39ADM0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Cysteine protease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_003250</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ADM0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02248">
        <property type="entry name" value="Peptidase_C1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.160:FF:000002">
        <property type="entry name" value="Cysteine protease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000068">
        <property type="entry name" value="Cysteine protease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.160">
        <property type="entry name" value="Granulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000118">
        <property type="entry name" value="Granulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037277">
        <property type="entry name" value="Granulin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025661">
        <property type="entry name" value="Pept_asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025660">
        <property type="entry name" value="Pept_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039417">
        <property type="entry name" value="Peptidase_C1A_papain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00396">
        <property type="entry name" value="Granulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00705">
        <property type="entry name" value="PAPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00277">
        <property type="entry name" value="GRAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57277">
        <property type="entry name" value="Granulin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00640">
        <property type="entry name" value="THIOL_PROTEASE_ASN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00639">
        <property type="entry name" value="THIOL_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041381763" description="Cysteine protease" evidence="8">
        <location>
            <begin position="29"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="443"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="11">
        <location>
            <begin position="52"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="10">
        <location>
            <begin position="140"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="Granulins" evidence="9">
        <location>
            <begin position="379"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9705110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="495" mass="55431" checksum="40D1A53127D26C40" modified="2024-01-24" version="1">MAVSQSPIMASFLFYFFFLLAALFSASAADMSIISYGDRLEKRTDAEVMAVYEAWLVKHGKSYNALGERERRFEIFKDNLRFIEEHNAVNRTYKVGLNRFADLTNEEYRSRYLGRRDETRRGLRASRVSDRYSFRAGEDLPESVDWREKGAVVPVKDQGNCGSCWAFSTVAAVEGINQIVTGDLISLSEQELVDCDKSYNQGCNGGLMDYAFEFIINNGGIDSEEDYPYRAADTTCDPNRKNARVVSIDGYEDVPQNDERSLKKAVANQPVSVAIEAGGRAFQLYQSGVFTGQCGTQLDHGVVAVGYGTEKGVDYWIVRNSWGPNWGESGYVKLERNLAGTETGKCGIAIEPSYPIKNGQNPPKPGPSPPSPSKPSVVCDEYYTCPEESTCCCIYEYSGFCFEWGCCPLEGATCCDDHYSCCPHEYPVCDVDAGTCLMVITTLLNRMFLVFFFHAIFVYLYFPMEQSKDNPLSVKAWRRTPARPIFAPRPVIEVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AFN0</accession>
    <name>A0AA39AFN0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_001657</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SINA (Seven in absentia) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AFN0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16571">
        <property type="entry name" value="RING-HC_SIAHs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03829">
        <property type="entry name" value="Sina"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000041">
        <property type="entry name" value="E3 ubiquitin-protein ligase SINAT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000483">
        <property type="entry name" value="E3 ubiquitin-protein ligase SINAT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.210.10:FF:000004">
        <property type="entry name" value="E3 ubiquitin-protein ligase SINAT5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.210.10">
        <property type="entry name" value="Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018121">
        <property type="entry name" value="7-in-absentia-prot_TRAF-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052088">
        <property type="entry name" value="E3_ubiquitin-ligase_SINA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049548">
        <property type="entry name" value="Sina-like_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008974">
        <property type="entry name" value="TRAF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013010">
        <property type="entry name" value="Znf_SIAH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10315">
        <property type="entry name" value="E3 UBIQUITIN PROTEIN LIGASE SIAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10315:SF161">
        <property type="entry name" value="RING-TYPE E3 UBIQUITIN TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21362">
        <property type="entry name" value="Sina_RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03145">
        <property type="entry name" value="Sina_TRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21361">
        <property type="entry name" value="Sina_ZnF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51081">
        <property type="entry name" value="ZF_SIAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="11">
        <location>
            <begin position="55"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="SIAH-type" evidence="12">
        <location>
            <begin position="108"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9706212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="317" mass="36096" checksum="F9CBC72FC7A1942E" modified="2024-01-24" version="1">MEIDSIECVSSTDGIYEEEIHHHHHHHFSSSSKPHSNVVSPAISPATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKSRVHNRCPTCRQELGDIRCLALEKVAESLELPCKYCSLGCPEIFPYYSKLKHEAQCNFRPYNCPYAGSECAIVGDIPFLVSHLRDDHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFNCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDEIEARNFSYSLEVGANGRKLIWEGTPRSIRDSHKKVRDSHDGLIIQRNMALFFSGGDRKELKLRVTGRIWKEQQNPDAGVCIPNLCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AFW4</accession>
    <name>A0AA39AFW4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Chromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_003632</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AFW4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045358">
        <property type="entry name" value="Ty3_capsid"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19259">
        <property type="entry name" value="Ty3_capsid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chromo" evidence="2">
        <location>
            <begin position="483"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="318"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="455"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="318"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="460"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9705658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="546" mass="62398" checksum="2976E4E2ADF7E5AE" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AG99</accession>
    <name>A0AA39AG99_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_003687</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AG99"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="169"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9705719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="180" mass="18834" checksum="AB28CA74BEC638D0" modified="2024-01-24" version="1">MEKQKKELTMLRYINSNDMKITPRTMAPCLLRTVHKYSQGLVVTGGGERNAIEGIVVGIVGMEGIVGKVVGSGGNVRFGIEGMVGMLGSGGSVALGKDGWVVDKDGSVGGSVGCGIGRVGSEGKGGNLGFGRVGITSVVGTEGGAVSKRRRAARLTSMLENESRTKRDNTRRKNVHHDTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AI59</accession>
    <name>A0AA39AI59_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_000237</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AI59"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041361544" evidence="2">
        <location>
            <begin position="26"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9708078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="120" mass="12351" checksum="852948B4EBE59258" modified="2024-01-24" version="1">MIERVYSVFLCFLLISVLFFPLAAAGRVLLGDEGGDHSTGRSKIDVKPSHSGQAGSALGHSSPSHATPSGVHTATANTANRAAQVVPCGSSAGQPFGANCVKPRQPERSCGCTNRGCIRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AI72</accession>
    <name>A0AA39AI72_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BCNT-C domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_002498</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AI72"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011421">
        <property type="entry name" value="BCNT-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027124">
        <property type="entry name" value="Swc5/CFDP1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48407">
        <property type="entry name" value="CRANIOFACIAL DEVELOPMENT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48407:SF1">
        <property type="entry name" value="CRANIOFACIAL DEVELOPMENT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07572">
        <property type="entry name" value="BCNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="BCNT-C" evidence="2">
        <location>
            <begin position="139"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9707495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="214" mass="24535" checksum="899F2C84D389F95F" modified="2024-01-24" version="1">MLTSMATTKELELFTISSVCDLEKKNKLLIETVKEVAAISKSEAQPEKSDVVWEQMNKGVSIKQDVQQKGSSIIENGTNDEVKRLTTAALSVVRMLQQLLQRYETLWVEIEVKKLVDVASEEASERAKGLAGTTSATEHNKGLEEELDAYKISDQYVEKASLLQRADYREFERERDAQLPLQARRKARYERGSMKVIPWFASFFFLIFFYFCCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AJ15</accession>
    <name>A0AA39AJ15_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Carboxypeptidase</fullName>
            <ecNumber evidence="10">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_000175</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the peptidase S10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="10"/>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9707982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AJ15"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11320:FF:000001">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000013">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033124">
        <property type="entry name" value="Ser_caboxypep_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF280">
        <property type="entry name" value="SERINE CARBOXYPEPTIDASE-LIKE 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00560">
        <property type="entry name" value="CARBOXYPEPT_SER_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 10" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041481314" description="Carboxypeptidase" evidence="10">
        <location>
            <begin position="25"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9707982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="475" mass="53474" checksum="FEC38700974C1F31" modified="2024-01-24" version="1">MVSMAYSLCNLLTFCLVLQAMTAAASVAGAEELQQEKDRVKDLPGQPAVEFRHYSGYVKLRPQDEKALFYWFFEAQGGVLEKPLVLWLNGGPGCSSIAYGAAQELGPFLVRSNGTQLVLNDFSWNKVANILFLEAPVGVGFSYTNKSSDLLELGDRITAEDSHAFLVQWFKRFPSLKTHDFYITGESYAGHYVPQLAELIYERNKRSSSKDSYINLKGFMIGNAVINDETDDMGLIEFAWSHAIISDQIYYGIMKNCDFKSDNLTNLCIKYAEGFLEAYSDIDVYSIYTPVCLISSNKTYRKLVTAPRLFTQHDLWHQLPSGYDPCTKDYAEKYFNREDVQKALHANVTKLPYPYTPCSNVIRQWNDSPDTMLPTIQKLLKAGLRIWVYSGDTDGRVPVTSTRYSINKMGLRIQQKWRAWFDSKQVAGWVVTYEGGLTLATVRGAGHQVPILAPAQSLALFSHFLSNATLPSSRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ALE7</accession>
    <name>A0AA39ALE7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Signal recognition particle subunit SRP72</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_001262</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the signal recognition particle (SRP) complex, a ribonucleoprotein complex that mediates the cotranslational targeting of secretory and membrane proteins to the endoplasmic reticulum (ER).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the SRP72 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ALE7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005786">
        <property type="term" value="C:signal recognition particle, endoplasmic reticulum targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000648">
        <property type="entry name" value="Signal recognition particle subunit SRP72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001600">
        <property type="entry name" value="Signal recognition particle subunit SRP72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013699">
        <property type="entry name" value="Signal_recog_part_SRP72_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026270">
        <property type="entry name" value="SRP72"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031545">
        <property type="entry name" value="SRP72_TPR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14094">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14094:SF9">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE SUBUNIT SRP72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08492">
        <property type="entry name" value="SRP72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17004">
        <property type="entry name" value="SRP_TPR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038922">
        <property type="entry name" value="SRP72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7 9" id="KW-0733">Signal recognition particle</keyword>
    <feature type="domain" description="Signal recognition particle SRP72 subunit RNA-binding" evidence="11">
        <location>
            <begin position="547"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="553"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="563"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="589"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="631"/>
            <end position="657"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR038922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9709696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="673" mass="73845" checksum="2DFF2C9C028B5C22" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D3T4</accession>
    <name>A0AA39D3T4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Root meristem growth factor 9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_027077</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D3T4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049306">
        <property type="entry name" value="GLV1-2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21529">
        <property type="entry name" value="GLV1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041412043" description="Root meristem growth factor 9" evidence="2">
        <location>
            <begin position="23"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9670913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="81" mass="9024" checksum="FA7D8AB669B8C855" modified="2024-01-24" version="1">MAPCKCLILLALLLCFFFFTTANATARSFGVMESDQYESAEKGHDSKFKPSEDGLPSEPEEIGDLAEMDYTPPRKKPPIHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D6B1</accession>
    <name>A0AA39D6B1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_025365</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D6B1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010333">
        <property type="term" value="F:terpene synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016102">
        <property type="term" value="P:diterpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00684">
        <property type="entry name" value="Terpene_cyclase_plant_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.600.10:FF:000007">
        <property type="entry name" value="Isoprene synthase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.130">
        <property type="entry name" value="Terpene synthase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034741">
        <property type="entry name" value="Terpene_cyclase-like_1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044814">
        <property type="entry name" value="Terpene_cyclase_plant_C1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001906">
        <property type="entry name" value="Terpene_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036965">
        <property type="entry name" value="Terpene_synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050148">
        <property type="entry name" value="Terpene_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005630">
        <property type="entry name" value="Terpene_synthase_metal-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225:SF221">
        <property type="entry name" value="(-)-GERMACRENE D SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225">
        <property type="entry name" value="OS04G0344100 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01397">
        <property type="entry name" value="Terpene_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03936">
        <property type="entry name" value="Terpene_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01019">
        <property type="entry name" value="Terpene_Cyclase_Like_1_C_Termi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01604">
        <property type="entry name" value="Terpene_Cyclase_Like_1_C_Termi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Terpene synthase N-terminal" evidence="5">
        <location>
            <begin position="9"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="Terpene synthase metal-binding" evidence="6">
        <location>
            <begin position="161"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9671620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="457" mass="53049" checksum="D05C2E7209DEC981" modified="2024-01-24" version="1">MYQTYTLVDDNDHLTTVSLLFRLLRQEGYHIPSDVFKKFMDEGGNFKESLVGDLPGMLALYEAAHLMVHGEDILDEALGFTTPHLQSMATDSDNPLTKQVIRALKHPIRKGLPRVEARHYINIYQEDDSHNKSLLKLAKLDYNMLQSFHRKELSEITKWWKGLDFATKLPFARDRIVEGYFWILGVYCEPQYYLARRILMKVFGVLSILDDIYDAYGTFEELKLFTEAIERWDASSIDQLPDYMKVCYQALLDVYEEMEEEMTKQGKLYRVHYAKAALKRQVQAYLLEAKWLKQEYIPTMEEYMGNALVSSAYSMLTTTSFVGMGDIVTKEAFNWVFSDPKMIRASNVIGRLMDDIVSHEFEQKRGHVASAVECYMKQYGVSKEEAYDEFKKQVENAWKDINEEFLQPTAVPVPLLTRVLNFSRMIDVLYKDEDEYTLVGPLMKDLVAGMLLDPVPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D7Q2</accession>
    <name>A0AA39D7Q2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Sodium/hydrogen exchanger</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_025763</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>K(+)(in) + H(+)(out) = K(+)(out) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:29467"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>Na(+)(in) + H(+)(out) = Na(+)(out) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:29419"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D7Q2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015386">
        <property type="term" value="F:potassium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015385">
        <property type="term" value="F:sodium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098719">
        <property type="term" value="P:sodium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018422">
        <property type="entry name" value="Cation/H_exchanger_CPA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004709">
        <property type="entry name" value="NaH_exchanger"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00840">
        <property type="entry name" value="b_cpa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110">
        <property type="entry name" value="SODIUM/HYDROGEN EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110:SF179">
        <property type="entry name" value="SODIUM_HYDROGEN EXCHANGER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01084">
        <property type="entry name" value="NAHEXCHNGR"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0050">Antiport</keyword>
    <keyword evidence="8 13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0630">Potassium</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="10 13" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="4 13 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 13" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="49"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="78"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="107"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="169"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="212"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="267"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="298"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="337"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="381"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="413"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="15">
        <location>
            <begin position="28"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9672277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="521" mass="58160" checksum="36B6C8D37EB87F72" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D8B0</accession>
    <name>A0AA39D8B0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Trimethylguanosine synthase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Cap-specific guanine-N(2) methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_023757</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)-ribonucleoside in snRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(2),N(7)-trimethyl 5'-triphosphoguanosine)-ribonucleoside in snRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:78479"/>
            <dbReference type="Rhea" id="RHEA-COMP:19087"/>
            <dbReference type="Rhea" id="RHEA-COMP:19089"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:167623"/>
            <dbReference type="ChEBI" id="CHEBI:172880"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:78480"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)-ribonucleoside in snoRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(2),N(7)-trimethyl 5'-triphosphoguanosine)-ribonucleoside in snoRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:78507"/>
            <dbReference type="Rhea" id="RHEA-COMP:19088"/>
            <dbReference type="Rhea" id="RHEA-COMP:19090"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:167623"/>
            <dbReference type="ChEBI" id="CHEBI:172880"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="3">
            <dbReference type="Rhea" id="RHEA:78508"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in snRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)-ribonucleoside in snRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:78471"/>
            <dbReference type="Rhea" id="RHEA-COMP:19085"/>
            <dbReference type="Rhea" id="RHEA-COMP:19087"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:156461"/>
            <dbReference type="ChEBI" id="CHEBI:172880"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:78472"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in snoRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)-ribonucleoside in snoRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:78475"/>
            <dbReference type="Rhea" id="RHEA-COMP:19086"/>
            <dbReference type="Rhea" id="RHEA-COMP:19088"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:156461"/>
            <dbReference type="ChEBI" id="CHEBI:172880"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:78476"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyltransferase superfamily. Trimethylguanosine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D8B0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071164">
        <property type="term" value="F:RNA cap trimethylguanosine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000305">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferase superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019012">
        <property type="entry name" value="RNA_cap_Gua-N2-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14741">
        <property type="entry name" value="S-ADENOSYLMETHIONINE-DEPENDENT METHYLTRANSFERASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14741:SF32">
        <property type="entry name" value="TRIMETHYLGUANOSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09445">
        <property type="entry name" value="Methyltransf_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WW" evidence="9">
        <location>
            <begin position="315"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="31"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00025783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00047418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9674394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="780" mass="86594" checksum="B513B3662BB04CC6" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DB75</accession>
    <name>A0AA39DB75_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_022187</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DB75"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000010">
        <property type="entry name" value="Aspartyl protease family protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000016">
        <property type="entry name" value="Probable aspartic protease At2g35615"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032799">
        <property type="entry name" value="TAXi_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032861">
        <property type="entry name" value="TAXi_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13683">
        <property type="entry name" value="ASPARTYL PROTEASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13683:SF274">
        <property type="entry name" value="PROTEIN ASPARTIC PROTEASE IN GUARD CELL 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14541">
        <property type="entry name" value="TAXi_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14543">
        <property type="entry name" value="TAXi_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041360963" description="Peptidase A1 domain-containing protein" evidence="8">
        <location>
            <begin position="25"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="9">
        <location>
            <begin position="160"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="178"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9677065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="496" mass="52953" checksum="3978A5AD6AF82716" modified="2024-01-24" version="1">MADKFFIFCIFAAFALSLSPFVFSRELSLDTDSHSSVLDVSGSIRKTLDVLSLKSSVSKPSDQGDEKTTSFSPSSLASSFSLELHPRELLHGGSHKDYRALMLSRLARDSARVKAINTKLQFAVTGTDKSDLVPMDTEMLHPQDFSTPVTSGTSQGSGEYFLRVGIGRPSKMFYMVIDTGSDVNWLQCKPCDDCYQQVDPIFDPAASSSFSRLGCQTPQCRNLDAFACRNDSCLYQVSYGDGSYTVGDFATETVSFGNSGSVDNVAIGCGHDNEGLFVGAAGLIGLGGGPLSLTSQIKASSFSYCLVNRDSVDSSTLEFNSARPSDAVTAPIFKNSKVDTFYYVGITGMSVGGEKLAIPPSIFEVDGSGKGGIIVDCGTAVTRLQTQAYNALRDTFVNLTKDLPSTSGFALFDTCYNLSSRTSVRVPTVAFLFDGGKSLALPPSNYLIPVDSAGTFCLAFAPTTASLSIIGNVQQQGTRVTYDLANSQVSFSSHKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DCG1</accession>
    <name>A0AA39DCG1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Trehalose 6-phosphate phosphatase</fullName>
            <ecNumber evidence="8">3.1.3.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_021043</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Removes the phosphate from trehalose 6-phosphate to produce free trehalose. Trehalose accumulation in plant may improve abiotic stress tolerance.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>alpha,alpha-trehalose 6-phosphate + H2O = alpha,alpha-trehalose + phosphate</text>
            <dbReference type="Rhea" id="RHEA:23420"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16551"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58429"/>
            <dbReference type="EC" id="3.1.3.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 8">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 8">Glycan biosynthesis; trehalose biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 8">Belongs to the trehalose phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.12" evidence="8"/>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DCG1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004805">
        <property type="term" value="F:trehalose-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01627">
        <property type="entry name" value="HAD_TPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1020:FF:000004">
        <property type="entry name" value="Trehalose 6-phosphate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000073">
        <property type="entry name" value="Trehalose 6-phosphate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000099">
        <property type="entry name" value="Trehalose 6-phosphate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044651">
        <property type="entry name" value="OTSB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003337">
        <property type="entry name" value="Trehalose_PPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00685">
        <property type="entry name" value="T6PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43768">
        <property type="entry name" value="TREHALOSE 6-PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43768:SF27">
        <property type="entry name" value="TREHALOSE-PHOSPHATE PHOSPHATASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02358">
        <property type="entry name" value="Trehalose_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0346">Stress response</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9679001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="385" mass="43364" checksum="DCD146E7E0E85587" modified="2024-01-24" version="1">MDLKSNHSAPVLTDPPPVNKSRFGMRSNLLPYSPPGAAFSSSLLLTIPRKKNGVLDDVRSSSWLDAMKSSSPTHKKITKDFNTEPASTDADVTYRTWLLKYPSALKSFEQITNYAKGKRIALFLDYDGTLSPIVDNPDRAFMSNSMRAAVRNVAKFFPTAIISGRSRDKVYNFVGLTELYYAGSHGMDIMGPVRHSISNGHPNCIRLTDKQGKEVNLFQPASEFLPMIDEVFRSLVSCTKEIKGAEVENNKFCVSVHYRNVDEKYWKTIVQCVDDILKDYPRLRLTHGRKVLEVRPVIDWDKGKAVEFLLESLGLSNSDDVLPIYVGDDQTDEDAFKVLRDGKRGYGILVSSVPKESNAFYSLRDPLEVMEFLKLLVMWKKSSAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DCQ5</accession>
    <name>A0AA39DCQ5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Response regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_022450</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DCQ5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009736">
        <property type="term" value="P:cytokinin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045279">
        <property type="entry name" value="ARR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43874">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43874:SF1">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR-LIKE APRR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="10"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9677467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="137" mass="15336" checksum="DAA7595EB82DE8E6" modified="2024-01-24" version="1">MCLVNTSKPKNLLCDANVNNCREVATLLRGCSYQVTSVGSTPEVIYVLNCEGASIDIVLAEADLPMANCMKILKYITQDQELRRIPVIMLVARDNISMIAKCLMHGAVDYLMMPLGSNGLFNLWTHVWRTRRMVSIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DD72</accession>
    <name>A0AA39DD72_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein SIEVE ELEMENT OCCLUSION B-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_022744</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DD72"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010088">
        <property type="term" value="P:phloem development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027944">
        <property type="entry name" value="SEO_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027942">
        <property type="entry name" value="SEO_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039299">
        <property type="entry name" value="SEOA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33232">
        <property type="entry name" value="PROTEIN SIEVE ELEMENT OCCLUSION B-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33232:SF12">
        <property type="entry name" value="PROTEIN SIEVE ELEMENT OCCLUSION B-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14577">
        <property type="entry name" value="SEO_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14576">
        <property type="entry name" value="SEO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sieve element occlusion N-terminal" evidence="1">
        <location>
            <begin position="27"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="Sieve element occlusion C-terminal" evidence="2">
        <location>
            <begin position="472"/>
            <end position="703"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9677947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="704" mass="80265" checksum="8E1DCFBDFBAA0284" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DDH1</accession>
    <name>A0AA39DDH1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_021581</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DDH1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000001">
        <property type="entry name" value="Ethylene-responsive transcription factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050913">
        <property type="entry name" value="AP2/ERF_ERF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31194:SF202">
        <property type="entry name" value="ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR ERF070"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31194">
        <property type="entry name" value="SHN SHINE , DNA BINDING / TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00367">
        <property type="entry name" value="ETHRSPELEMNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="7">
        <location>
            <begin position="113"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="188"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="218"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="231"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9679705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="341" mass="38180" checksum="8A1632C2186BFAEF" modified="2024-01-24" version="1">MDQSLLYPIKYTEHRSITNKQTRQSRRPPEQNSGVARVVRISVTDPDATDSSSDEEDELFSRQRVKRYTNEINIATVSKSIAPVAGRKRGAGAGEMAPCRRPMKVAAPTNGRKFRGVRQRPWGKWAAEIRDPARRVRLWLGTYDTAEEAAMVYDNAAIKLRGPDALTNFVTPPPPPKPEINLTAVSGYESGDESRNLSSPTSVLHFRTHSSSTDEAELSNRSDPVHETESSEPVQNPETHQQQPVQTVREECQGETTNTGDSSVDYLPMDLPFIDDMFNFEAPGSIFEDTSILPESILNEDFSDMFLDSPLNFDISSSWRLEDDYFQDIGDLFTSDSLSVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DE06</accession>
    <name>A0AA39DE06_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Disease resistance protein RGA3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_019542</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DE06"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14798">
        <property type="entry name" value="RX-CC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038005">
        <property type="entry name" value="RX-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766:SF61">
        <property type="entry name" value="NB-ARC DOMAIN DISEASE RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766">
        <property type="entry name" value="PLANT BROAD-SPECTRUM MILDEW RESISTANCE PROTEIN RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="6">
        <location>
            <begin position="12"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="5">
        <location>
            <begin position="168"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="7">
        <location>
            <begin position="425"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="8">
        <location>
            <begin position="567"/>
            <end position="764"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9680260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="892" mass="101896" checksum="C54D972E06BD467B" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DER3</accession>
    <name>A0AA39DER3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DRBM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_018223</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DER3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19907">
        <property type="entry name" value="DSRM_AtDRB-like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19908">
        <property type="entry name" value="DSRM_AtDRB-like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000036">
        <property type="entry name" value="Double-stranded RNA-binding protein 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044450">
        <property type="entry name" value="AtDRB-like_DSRM_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044451">
        <property type="entry name" value="AtDRB-like_DSRM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46031">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46031:SF40">
        <property type="entry name" value="DRBM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00358">
        <property type="entry name" value="DSRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50137">
        <property type="entry name" value="DS_RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DRBM" evidence="5">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="5">
        <location>
            <begin position="87"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="190"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="334"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="460"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="517"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="341"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9682238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="543" mass="60426" checksum="0F9168B602513859" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DEY8</accession>
    <name>A0AA39DEY8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">LIM zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_020428</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Interacts with F-actin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9681551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DEY8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051017">
        <property type="term" value="P:actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd09440">
        <property type="entry name" value="LIM1_SF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09441">
        <property type="entry name" value="LIM2_SF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000002">
        <property type="entry name" value="LIM domain and actin-binding 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24206">
        <property type="entry name" value="OS06G0237300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5 8" id="KW-0440">LIM domain</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="10">
        <location>
            <begin position="8"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="10">
        <location>
            <begin position="105"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="180"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="188"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9681551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="234" mass="25495" checksum="2427A306C7528E4D" modified="2024-01-24" version="1">MAFTGTLDKCKACDKTVYVVDLLSADGASYHKTCFKCSHCKGTLVMSNYSSMDGVLYCKPHFEQLFKESGNFSKNFQTSAKPADKLNELSRAPSKLSSMFSGTQDKCSACRKTVYPLEKVTLEGESYHKSCFKCAHGGCPLTHSSYAALNGILYCKHHFSQLFMEKGNYSHVLEAATHKKQAASNAEAEAKAEAETKAEAEGEAETKAEAEGEGEAKAEAEPEHDQPDQTQGQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DFN1</accession>
    <name>A0AA39DFN1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein TRIGALACTOSYLDIACYLGLYCEROL 4, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_019688</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DFN1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009941">
        <property type="term" value="C:chloroplast envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070300">
        <property type="term" value="F:phosphatidic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034196">
        <property type="term" value="P:acylglycerol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990052">
        <property type="term" value="P:ER to chloroplast lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044160">
        <property type="entry name" value="TGD4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34954">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34954:SF3">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9680437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="464" mass="51549" checksum="E4B93B801E0A830A" modified="2024-01-24" version="1">MANLRTAMDAAFWDLDISSPQTLHGAARAVPGDPFPLEGARASRALRVQQLSFLGNGFPLGIIPSFSPTSQKDLGSFSLQSLFLRPSTSNWWLGLTGQFRPKKLMSSIKADLSAVDEWELSTFKEVAKHFIDKSLFSFGLCSQLSLTSASSLMVSTEQHGEKKGRRNRVMLFHQLPFHDITLEAAWPELFIDHKGRYWEVPESISLGLSSLVSESGLRYRFGIHKNGGHPQSVNAIYDEAPSALMPGLCAKAAFSYEKSRDLWRQREKQEDGIVKTERGLVWRPSYDIRLREPHAAISGIIGGTCEAWFGGSREHGDGSSADAKKRSPFGADLFASACCTFQHGQFRKRYGDLTRVDARLNICSASALAKRVSNLFSSSVNGAKDPLSSPRLNLIFQQQVAGPIVFRVDSKFLLDSSGGRAGPHLEDFTYSLNYSLRLLRSGKVVAWYSPKRKEGMIELRLFEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DGK4</accession>
    <name>A0AA39DGK4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">CRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_018727</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DGK4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000373">
        <property type="term" value="P:Group II intron splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.110.60:FF:000003">
        <property type="entry name" value="CRM-domain containing factor CFM3B, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.110.60:FF:000002">
        <property type="entry name" value="CRS2-associated factor 1, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.60">
        <property type="entry name" value="YhbY-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045278">
        <property type="entry name" value="CRS1/CFM2/CFM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001890">
        <property type="entry name" value="RNA-binding_CRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035920">
        <property type="entry name" value="YhbY-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31846:SF10">
        <property type="entry name" value="CHLOROPLASTIC GROUP IIA INTRON SPLICING FACILITATOR CRS1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31846">
        <property type="entry name" value="CRS1 / YHBY (CRM) DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01985">
        <property type="entry name" value="CRS1_YhbY"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01103">
        <property type="entry name" value="CRS1_YhbY"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75471">
        <property type="entry name" value="YhbY-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51295">
        <property type="entry name" value="CRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="3" id="KW-0934">Plastid</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="CRM" evidence="13">
        <location>
            <begin position="201"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="CRM" evidence="13">
        <location>
            <begin position="421"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="domain" description="CRM" evidence="13">
        <location>
            <begin position="634"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="8"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="99"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="575"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="8"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9682855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="829" mass="93828" checksum="9DDD3E8489BFE3DF" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DH81</accession>
    <name>A0AA39DH81_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_016485</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DH81"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9684011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="66" mass="7532" checksum="76443264F033BFB2" modified="2024-01-24" version="1">MNQGNTNPNAIPMCLTEATKGKIQVGDQEYSHLKTHINPVPRTDSRNEVYMTSFTQNPQKHQKYVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DM73</accession>
    <name>A0AA39DM73_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SPX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_014348</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DM73"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016036">
        <property type="term" value="P:cellular response to phosphate starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14481">
        <property type="entry name" value="SPX_AtSPX1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031142">
        <property type="entry name" value="SPX_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45978:SF5">
        <property type="entry name" value="SPX DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45978">
        <property type="entry name" value="SPX DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03105">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SPX" evidence="4">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="domain" description="SPX" evidence="4">
        <location>
            <begin position="114"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="36"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="198"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="36"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9688645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="293" mass="33483" checksum="8158331833121E21" modified="2024-01-24" version="1">MKFGKSLSNQIEETLPEWRDKFLSYKELKKQLKLIDPKAGDRPNKRLRLDAGNSSEAGEKEAGDMTKEEVDFIKLLEDELEKFNTFFVEKEEEYIIRLKELQDRVAEANGYNEEMIKIRKEIVDFHGEMVLLENYSALNYTGLAKILKKYDKRTGALIRLPFIQKVLQQPFFTTDLLYKLVKECEAMLDRLFPTNELPASTGATDGQEGCGDPTTTATPQSDALLRMPKELAEIEYMESLCMKSTIAALRALKEIRSKSSTVSVFSLPPLQISGLEDTWKKIPELPVLEQEAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DN50</accession>
    <name>A0AA39DN50_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_013157</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DN50"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.330:FF:000001">
        <property type="entry name" value="Protein SRG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050295">
        <property type="entry name" value="Plant_2OG-oxidoreductases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47991">
        <property type="entry name" value="OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="6">
        <location>
            <begin position="209"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9690863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="366" mass="41229" checksum="9DC955F140A04971" modified="2024-01-24" version="1">MDAQKLTSLGGSLPVPSVQELAKKTLTTVPPRYLRPEQDPPFLSDSSTTCSSIHVPIIDFHRLVSGDVLDSELDKLHNACKDWGFFQLINHGVSSALVEKVKVEIQEFFNLPMKEKKKFWQQPEDLEGFGQAFVVSEEQKLDWADMFYMITLPTYLRKPHLFPKLPISLRETLEVYAVQLRNLAMTILGFLAKALKMEASEMRELFEEGHQGMRMNYYPPCPQPDQVIGLTPHSDAVGLTILLQVNEMEGLQIRKEGMWIPIKPLPGAFIVNIGDILEIVTNGTYRSIEHRATVNSIKERLSIATFYSAKLNGDMGPAPSLVSPDSPALFKRIGVSDYFKGLFSRELNGKSYLEVLRSETGEVQNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DNA1</accession>
    <name>A0AA39DNA1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">WRKY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_013196</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DNA1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.80:FF:000003">
        <property type="entry name" value="WRKY transcription factor 57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.80">
        <property type="entry name" value="WRKY domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003657">
        <property type="entry name" value="WRKY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036576">
        <property type="entry name" value="WRKY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044810">
        <property type="entry name" value="WRKY_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31221:SF320">
        <property type="entry name" value="WRKY TRANSCRIPTION FACTOR 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31221">
        <property type="entry name" value="WRKY TRANSCRIPTION FACTOR PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03106">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00774">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118290">
        <property type="entry name" value="WRKY DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50811">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="WRKY" evidence="7">
        <location>
            <begin position="175"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="81"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="115"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="129"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="152"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9690926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="319" mass="34949" checksum="3BD8AD59EC282443" modified="2024-01-24" version="1">MSDEPRDLYYHDPFHDDRHGIIGNTGFSFSAINDSKADSSMTAAPSPPNLQGFDPPYMSFTDCLNGSLDYNSLTTAFGLSPSSSEAFSPVEGNHKPVANLGDLGASGEIAGTPNSSISSSSTEAGAEEDSSKSKKDRQAKVSELDGGDGSKKVNKPKKKAEKRQREPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCTTQKCTVKKRVERSYQDPSTVITTYEGQHNHQIPVTLRGNAGGMLPPSVLTPGQMGGPGFPQELFFQMASPMTNLSAAGSFYPQGLTPFQQLQFHDYGLLQDVVPSMIHKQEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DQ62</accession>
    <name>A0AA39DQ62_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_013763</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DQ62"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000869">
        <property type="entry name" value="Zinc finger CCCH domain-containing protein 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044715">
        <property type="entry name" value="WDR86-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44489">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44489:SF1">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 7" id="KW-0853">WD repeat</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="10">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="10">
        <location>
            <begin position="151"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="316"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="8">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="8">
        <location>
            <begin position="151"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="76"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="79"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="99"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="111"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="133"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9691675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="484" mass="53568" checksum="257A4B8E06D223B1" modified="2024-01-24" version="1">MFYLCIFCDIAMDIKPSRLVGHKNERVRRHVNNIVCSYWLRGRCNRNPCRFLHQELPQNTHYQVSNQFRKNCWQRNPDSDSMSASSLTSLDRSSGSTTPKRSSASNQSHRNNRERTLYSDSKGEWDSTNLRCSSSPTKAGSSSGNGITQKAIGERVCKYWLHGNCVEADKCQYLHSWFKGHGVFKLAELNGHIKVVGAAISGIVLPSGSEKLYTASRDGYIRVWDCHTGQCDGVVNLGGEIGSLISAGPWVFAGIKDVVKAWNIESCAELSLDGPVGQIYAMVVNNDMLFAGAQNGTIYAWKANKETNAFEPATTLEGHNGAVVSLTVGGGRLYSGSMDNTIRVWDLDTLQCIHTLKDHASVVMSLVCWGPYLISCSLDQTIKVWLATEAGSLEVTYTHNEEHGVLSLFGMNNLKGKPILLCSCNDNSVRLYELPSFTEKGRIFAKEEVREIQIGPRGLVFTGDGTGQVDVWKWQAEPTSLTFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DQS9</accession>
    <name>A0AA39DQS9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4283 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_009528</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9693608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DQS9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025558">
        <property type="entry name" value="DUF4283"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34427">
        <property type="entry name" value="DUF4283 DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34427:SF5">
        <property type="entry name" value="DUF4283 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14111">
        <property type="entry name" value="DUF4283"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4283" evidence="2">
        <location>
            <begin position="124"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="359"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9693608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="385" mass="43050" checksum="EC60BF398173CF67" modified="2024-01-24" version="1">MGPESLGFLREGLTHCIEDGGDGKWGREWKEKGRTYSLTRGANKAGWYLRLGVADSERRRFSIFIPKGKGDKGGWVSMEKELQKLEGAFGKKADHQETREGTNWRNTNSVRVKIKREEMKGNLQKLENCVVVSWNSRSEGEEDLENLGRIWANSWGLKGSLGLARMERNRALLEFEIPEEARRVVSYGSRWLGGFQEEVSSEIWVRIVGLPISLWTPTILKRVGEECGGLVAVDPLTECLGELQWARILVKARGDVLPSVLEAEIEEETYVLSLWWEFSPVLRKKSVGCSPENGRKSGEVRDDISSRVGMRVEEGLGGRIETLQMAADLMEEPEHGPGWQKGPSLGLKVVARLDGPASTNRLMGSNLIKRRTEGDEADEGPSARS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DRS1</accession>
    <name>A0AA39DRS1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GDSL esterase/lipase 1-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_011113</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9691837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DRS1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016298">
        <property type="term" value="F:lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044552">
        <property type="entry name" value="GLIP1-5/GLL25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008265">
        <property type="entry name" value="Lipase_GDSL_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45966">
        <property type="entry name" value="GDSL-LIKE LIPASE/ACYLHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45966:SF34">
        <property type="entry name" value="GDSL-LIKE LIPASE_ACYLHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01098">
        <property type="entry name" value="LIPASE_GDSL_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041384711" description="GDSL esterase/lipase 1-like" evidence="3">
        <location>
            <begin position="25"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9691837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="367" mass="40883" checksum="0F78F27467C458FC" modified="2024-01-24" version="1">MKSSGISIIHALVFCACLLIPSRGLSHPHQTQKHVAFFIFGDSLFDAGNNKYINTTDQRANFWPYGETFFGHPTGRFSDGRLIPDFIAEYAKLPFLPPYLQPGSNQLTYGANFAFAGAGALDETYQGKVINLNTQLTYFKNMEKLLRQKLGDEAAKKILLEAVYLISIGTNDYLSPYFTNSSVLQSYPQKLYRHMVIGNLTVVIKEIYEQGGRKLGVLSLGPLGCIPAMKAIKKPGTGECIEEASEQAKLHNKALSKVLQKLESKLKGFKYSMFDFYTTFEDRMNNPSKYGFKEGKTACCGSGPYRALVSCGGEGTMKEYELCGNVREYVFFDGGHPTDKANQEMAKLMWSGTHNITGPYNLKELFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DSC9</accession>
    <name>A0AA39DSC9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_011489</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DSC9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000348">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46225">
        <property type="entry name" value="C3H4 TYPE ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46225:SF19">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="230"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="260"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="4">
        <location>
            <begin position="361"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="35"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="169"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="409"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="35"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="65"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="169"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="410"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9692452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="446" mass="49502" checksum="DE51D1EE9F790935" modified="2024-01-24" version="1">MAIPSLEPHSRSQTYKYPLLMERTENHGDREHIIDITRNDDASSSSSHDDHHPVMNPPQHEERPSSSTQTPTNQTSSSSNRLNSRNSSFMRRSDGYSRRRRSPLNSGLWISVELVVTVSQIIASIVVLSLSRRENPRAPLFAWVVGYASGCFATLPILYWRYRTRNQSNEQESSQSRQVSSQNNPSSEPTSYTAISVNQASETARAATRRSRFVGIFHMRFSGMVDHFKMALDCFFAVWFVVGNVWIFGGHSSPSDAPKLYRLCIVFLTFSCIGYAMPFILCATICCCLPCIISILGFREDLSQTRGAAPESIDALPTYKFKLKKNGDVDDQEVNSGAGEGGVLAAGTEKERAISGEDAVCCICLAKYADNDELRELPCCHFFHVECVDKWLKINALCPLCKFEVGGTSGASPNATSTSGHQRERPTAGLQTFKWRSSCASTPFAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DV28</accession>
    <name>A0AA39DV28_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_007228</name>
        <name evidence="4" type="ORF">PVL29_007229</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DV28"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0195">Cyclin</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="2">
        <location>
            <begin position="15"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9698018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9698019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="122" mass="13725" checksum="2AAF822690BF8082" modified="2024-01-24" version="1">MVLPEGVRARQDAANGMLKVHSYHNFRPETAYLSVTHLDRFLCTYDLSGKEWSLQLLSVPCIALAVKMEGRSAPLPLDLQVMEPKFLFTAMTGQQMELLVMAVFKCFTVPAMFRISFSPPAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DVI8</accession>
    <name>A0AA39DVI8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Disease resistance protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_008017</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Confers resistance to late blight (Phytophthora infestans) races carrying the avirulence gene Avr1. Resistance proteins guard the plant against pathogens that contain an appropriate avirulence protein via an indirect interaction with this avirulence protein. That triggers a defense system including the hypersensitive response, which restricts the pathogen growth.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9699206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DVI8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009626">
        <property type="term" value="P:plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14798">
        <property type="entry name" value="RX-CC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038005">
        <property type="entry name" value="RX-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF1152">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0381">Hypersensitive response</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0611">Plant defense</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="9">
        <location>
            <begin position="6"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="8">
        <location>
            <begin position="184"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="10">
        <location>
            <begin position="437"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="11">
        <location>
            <begin position="553"/>
            <end position="898"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9699206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="936" mass="107085" checksum="6080863C798BA3ED" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DWG3</accession>
    <name>A0AA39DWG3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Gamma-tubulin complex component</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_008649</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the gamma-tubulin ring complex (gTuRC) which mediates microtubule nucleation.</text>
    </comment>
    <comment type="function">
        <text evidence="6">Gamma-tubulin complex is necessary for microtubule nucleation at the microtubule organizing centers (MTOCs).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cytoplasm</location>
            <location evidence="1 7">Cytoskeleton</location>
            <location evidence="1 7">Microtubule organizing center</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the TUBGCP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DWG3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000930">
        <property type="term" value="C:gamma-tubulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043015">
        <property type="term" value="F:gamma-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051011">
        <property type="term" value="F:microtubule minus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051225">
        <property type="term" value="P:spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1900:FF:000010">
        <property type="entry name" value="Gamma-tubulin complex component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1900">
        <property type="entry name" value="Gamma-tubulin complex, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007259">
        <property type="entry name" value="GCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040457">
        <property type="entry name" value="GCP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042241">
        <property type="entry name" value="GCP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041470">
        <property type="entry name" value="GCP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302">
        <property type="entry name" value="GAMMA TUBULIN COMPLEX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302:SF27">
        <property type="entry name" value="GAMMA-TUBULIN COMPLEX COMPONENT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04130">
        <property type="entry name" value="GCP_C_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17681">
        <property type="entry name" value="GCP_N_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4 7" id="KW-0493">Microtubule</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gamma tubulin complex component protein N-terminal" evidence="9">
        <location>
            <begin position="2"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="Gamma tubulin complex component C-terminal" evidence="8">
        <location>
            <begin position="399"/>
            <end position="723"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00053139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF17681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9696532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="743" mass="85868" checksum="7D86DB3E38ACB0FC" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DWJ3</accession>
    <name>A0AA39DWJ3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">sulfate adenylyltransferase</fullName>
            <ecNumber evidence="3">2.7.7.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_005991</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>sulfate + ATP + H(+) = adenosine 5'-phosphosulfate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:18133"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58243"/>
            <dbReference type="EC" id="2.7.7.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the sulfate adenylyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.4" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DWJ3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004020">
        <property type="term" value="F:adenylylsulfate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004781">
        <property type="term" value="F:sulfate adenylyltransferase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000103">
        <property type="term" value="P:sulfate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00517">
        <property type="entry name" value="ATPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.400.10:FF:000002">
        <property type="entry name" value="ATP sulfurylase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000006">
        <property type="entry name" value="bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.400.10">
        <property type="entry name" value="Sulfate adenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025980">
        <property type="entry name" value="ATP-Sase_PUA-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024951">
        <property type="entry name" value="Sulfurylase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002650">
        <property type="entry name" value="Sulphate_adenylyltransferase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00339">
        <property type="entry name" value="sopT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11055">
        <property type="entry name" value="BIFUNCTIONAL 3'-PHOSPHOADENOSINE 5'-PHOSPHOSULFATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11055:SF41">
        <property type="entry name" value="SULFATE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01747">
        <property type="entry name" value="ATP-sulfurylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14306">
        <property type="entry name" value="PUA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="5" id="KW-0934">Plastid</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="ATP-sulfurylase PUA-like" evidence="14">
        <location>
            <begin position="55"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="Sulphate adenylyltransferase catalytic" evidence="13">
        <location>
            <begin position="227"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF14306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9700466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="450" mass="50653" checksum="BFE1AB14F57899FF" modified="2024-01-24" version="1">MASISTLFTKTPNPSPSLCLPRTSKSHFTPAFRLPIPLHSKKRTHQKLRVSSALIEPDGGKLVELFVEESLRDVKKREALRMPRIKLSRIDLEWVHVLSEGWASPLRGFMRESEFLQTLHFNSLRLDDGSFVNMSVPIVLAIDDADKHQIGDSTKVALVDSKDNTIAILSSIEIYKHHKEERIARTWGTTAPGLPYVDQAIAKSGNWLIGGDLEVVEPVKYNDGLDRFRLSPAELREEFTRRNADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPVLLLHPLGGYTKADDVPLSWRMKQHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHPVEKRDLYDADHGKKVAAYDQTQNKMAFFDPSRAQDFLFISGTKMRTLAKNRENPPDGFMCPGGWQVLVEYYDSLVPSNNGKVPETVPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DXG8</accession>
    <name>A0AA39DXG8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">NB-ARC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_006784</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DXG8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF955">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="5">
        <location>
            <begin position="155"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="4">
        <location>
            <begin position="308"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="6">
        <location>
            <begin position="542"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="7">
        <location>
            <begin position="666"/>
            <end position="957"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9701561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="982" mass="112361" checksum="C7CAF510CBED5729" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DXJ1</accession>
    <name>A0AA39DXJ1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_005538</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9699714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DXJ1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14241:SF24">
        <property type="entry name" value="G DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14241">
        <property type="entry name" value="INTERFERON-INDUCED PROTEIN 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="356"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9699714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="390" mass="44124" checksum="308875D539F95E2F" modified="2024-01-24" version="1">MTGEFFSNEEDETQSSSSRSAFCWWRTAQEFQDHARLKLDLHTLSHLTPRRRLLRELERLALVADQGLDELRHKLLTYRSGDFWLPTGGISKADMDVPPVITILVLGLSGSGKSSLVNLMYSVLGRSGLIPFAQTSSNVGLHMGSLSLSLSMVSVFLHSGESSNYTTMFLEEHNVLRSVRSGFCVFDTRGLDLNRMREGLEEISSWMADGVRHYQPCSRPEDDATTPPMGCQLSSARFTPRPVNCAVLVVNLAEITKAFNNRDLNPVEATRDLFLCPAIRKSNEKPLLILTHGDQLKPEERIGGRLKLCEYLGVAEATCIRYTYDSVTSYALTEAVYRVLIQSDRTHLPKRKPRDWLLIFWSWIICSISAFFAFLAYFFSKLGRKNIQRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DXS6</accession>
    <name>A0AA39DXS6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GATA-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_007126</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9697852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DXS6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="5">
        <location>
            <begin position="146"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9697852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="179" mass="20033" checksum="86BB80CB4DD689B1" modified="2024-01-24" version="1">MDSRWPPHSSENKHLARETRDFDLNIAENEPLDMSLGFNATANGQSYSNSPQVPILTTQNHYQNADASYSQAKNNFPSSSLLIPGYYDCLNQCFIPSATAVSPPPVNNHSVFGNPLRGRQVEPGSLGQDYYEWQVMQVNNSSIPIRRCANCHTTDTPMWRRGPGGPKVNPLQHEYVLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E1Y1</accession>
    <name>A0AA39E1Y1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Transmembrane and coiled-coil domain-containing protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_005099</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMCO4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E1Y1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007941">
        <property type="entry name" value="DUF726"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17920:SF3">
        <property type="entry name" value="TRANSMEMBRANE AND COILED-COIL DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17920">
        <property type="entry name" value="TRANSMEMBRANE AND COILED-COIL DOMAIN-CONTAINING PROTEIN 4 TMCO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05277">
        <property type="entry name" value="DUF726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="303"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="351"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="469"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="30"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9703645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="662" mass="71894" checksum="3798A12D986DFD98" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E2J9</accession>
    <name>A0AA39E2J9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EamA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_004483</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the drug/metabolite transporter (DMT) superfamily. Plant drug/metabolite exporter (P-DME) (TC 2.A.7.4) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E2J9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23051:SF9">
        <property type="entry name" value="EAMA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23051">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35, MEMBER F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="147"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="172"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="199"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="237"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="273"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="326"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="365"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="4">
        <location>
            <begin position="139"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9702772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="397" mass="43372" checksum="E88E499C517AA301" modified="2024-01-24" version="1">MMGWKYKAGLGLIGTVVFIWVASAEITQRIFSEYKQPFALTYLGVSLMVVYLPIAVLKDWICSRLDINSFKNLCNGSTIAKSSAGLDVPLRINEICSSSETDTESSLNTDMDLSENEDGWLIITNMEDEVGLLEKNHELSSWEIAKCGFYLAPIWFITEYLSNSALANTSVASTTVLTSTSGLFTLFFGALLGQDTVNITKVVAVFISMAGVAMTTVGKTWAPDEMLSASESRKHSIIGDIFGLLSAVSYGLFTVLLKRSAGSEGDKVDVQKFFGYIGLFTLLGFWWLAWPLNAVGVEPQFELPHSTSIGEVVLLNGFVGSVLSDYFWALSVVWTTPLVATLGMSLTIPLAMIADMMIHGRRYSAVYIFGCIEVFAGFVIANLSDKFSPKAESSYSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E4F9</accession>
    <name>A0AA39E4F9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cytochrome P450 76AD1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_001946</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E4F9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24299">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24299:SF59">
        <property type="entry name" value="CYTOCHROME P450 SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041218856" description="Cytochrome P450 76AD1-like protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9706719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="206" mass="22689" checksum="89F58BB72BF852FD" modified="2024-01-24" version="1">MDSLRCLLLCLLIAWTSIYIMGSARRSSKSSASKLPPGPAPLPIIGNLLKIGNKPHESLANLAKTHGPIMSLKLGCVYTIVISSPAMAKEVLQKHDLSFCNRSIPDAGRASNHNQHSIAWLPVSTWWRILRRICNSNIFTTQKLNSNQHIRTQKVHELLHSVEQSCQVGAAVNIGQEAFRTALNLLSNTIFSIDLVDPSSQTAKEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E4L5</accession>
    <name>A0AA39E4L5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Extra-large guanine nucleotide-binding protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_003099</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the G-alpha family. XLG subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E4L5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031683">
        <property type="term" value="F:G-protein beta/gamma-subunit complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00066">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.400.10:FF:000005">
        <property type="entry name" value="Extra-large guanine nucleotide-binding protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000692">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.400.10">
        <property type="entry name" value="GI Alpha 1, domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001019">
        <property type="entry name" value="Gprotein_alpha_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011025">
        <property type="entry name" value="GproteinA_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053057">
        <property type="entry name" value="XLG_GTP-binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36486">
        <property type="entry name" value="OS01G0977800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36486:SF4">
        <property type="entry name" value="PH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00503">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00318">
        <property type="entry name" value="GPROTEINA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00275">
        <property type="entry name" value="G_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47895">
        <property type="entry name" value="Transducin (alpha subunit), insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51882">
        <property type="entry name" value="G_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="7 11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="104"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="168"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="566"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="176"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="191"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="514"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="684"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="789"/>
            <end position="792"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00060880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9704887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="903" mass="100739" checksum="31F9B2BFDF6E6FB2" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YI10</accession>
    <name>A0AA38YI10_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Cytochrome P450 CYP736A12-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_027019</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YI10"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11072">
        <property type="entry name" value="CYP71-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000011">
        <property type="entry name" value="Cytochrome P450 83B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47943:SF2">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47943">
        <property type="entry name" value="CYTOCHROME P450 93A3-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10 11" id="KW-0349">Heme</keyword>
    <keyword evidence="7 10 11" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 11" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="10">
        <location>
            <position position="438"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9670830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="498" mass="56265" checksum="6281C087D1986D3A" modified="2024-01-24" version="1">MDWSWIALSLIAVAYVVRAFLNISKNKHKRLPPGPIGIPILGNLHMLGELPHQDLHRLAKNYGPIMYMHFALVPTIVVSSPQAAEQFLKTNDLVFSGRPPHEGSRIVSYDRKGISFTDYGPYWRNMRKLCTLGLLSNLRISSFQPLRREELDLLIKSLKEAALARTAVDLSAKISSLSADMSCRMIFGKKYMDKDIDQRGFKAVIQEGMQLAAAPNIGDYIPFVAPLDLQGLARRMKAISKVFDAFFEKIIDEHIHEPKEEGQPKDLIDVMLGYMGSKENEFQIERSNIKAIVLDMLAGSMDTSATAIEWALAELLKNPRIMKKVQEELEKVVGMERKVEESDLESLEYLDMVVKETLRLHPVAPLLIPHESLEDCTINGFHIPQKSRVMVNVYAIGRDPNVWTDVEKFLPERFIGSSIDLRGRDFQLIPFGSGRRGCPGMQLGLTVVRLVLAQLVHCFDWELPNGMMSSELDMTEEFGLTVPRAKHLLAVPTYRLPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YIB6</accession>
    <name>A0AA38YIB6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL11m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_027104</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the universal ribosomal protein uL11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YIB6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00349">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1550.10:FF:000005">
        <property type="entry name" value="50S ribosomal protein L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.250:FF:000003">
        <property type="entry name" value="Mitochondrial ribosomal protein L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.250">
        <property type="entry name" value="Ribosomal protein L11, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1550.10">
        <property type="entry name" value="Ribosomal protein L11/L12, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00736">
        <property type="entry name" value="Ribosomal_uL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000911">
        <property type="entry name" value="Ribosomal_uL11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006519">
        <property type="entry name" value="Ribosomal_uL11_bac-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020783">
        <property type="entry name" value="Ribosomal_uL11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036769">
        <property type="entry name" value="Ribosomal_uL11_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020784">
        <property type="entry name" value="Ribosomal_uL11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036796">
        <property type="entry name" value="Ribosomal_uL11_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01632">
        <property type="entry name" value="L11_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL11M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00298">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03946">
        <property type="entry name" value="Ribosomal_L11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00649">
        <property type="entry name" value="RL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54747">
        <property type="entry name" value="Ribosomal L11/L12e N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46906">
        <property type="entry name" value="Ribosomal protein L11, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL11 N-terminal" evidence="7">
        <location>
            <begin position="17"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL11 C-terminal" evidence="6">
        <location>
            <begin position="80"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9670956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="155" mass="16941" checksum="23E9AFB6D6E6C030" modified="2024-01-24" version="1">MSSVKEILTRRPVSATIRLTVPAGAARPAPPVGPALGQYRLNLMAFCKDFNARTQKYKPDTPMAVTITAYKDNTFEFTVKSPSVTWYLKKAAGIELGSGRPGHVVASSVTLKHIYEIAKVKQSDPYCQYMSLESICKSIIGTANTMGIKVVKELD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YIJ4</accession>
    <name>A0AA38YIJ4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dirigent protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_027216</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant dirigent protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9671116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YIJ4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004265">
        <property type="entry name" value="Dirigent"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21495">
        <property type="entry name" value="NUCLEOPORIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03018">
        <property type="entry name" value="Dirigent"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0052">Apoplast</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0964">Secreted</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="76"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9671116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="98" mass="10835" checksum="34088EC411A6894E" modified="2024-01-24" version="1">MTSKSATVFCAEFMMDDPLTVGPEPSSKLVGRAQGIYASASQEEMGLLMYSQCFGPKHHLFKGAGDADRRRADGEICILGTICFLGMYWRIYGKPNFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YJF9</accession>
    <name>A0AA38YJF9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycosyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_025315</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YJF9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035251">
        <property type="term" value="F:UDP-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000037">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050481">
        <property type="entry name" value="UDP-glycosyltransf_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48049">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48049:SF91">
        <property type="entry name" value="UDP-GLYCOSYLTRANSFERASE 79B7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9671553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="389" mass="42884" checksum="086738FAD30E5ED8" modified="2024-01-24" version="1">MAMANSSKFHVVMFPWFATTSPSSILPKKAQPQLQHLNLHPTLISFHPLTIPHVDGLPPGAETASDVPIFLHHLLATAMDRITDQVEAALRALKPDFFPVCAAAFAYNFVPAQQIRLKLANSSWPPPGYPSSTVVLGPHEARLLQFLLFPFGEDITFHKRLTAAIKRGDIVAIGTCQEVKGPFCGYMERQFGKPMFVTGPVLLAPSPLAPEDRRAQWLGGFKPGSVIFCAFGSQNFPEKDRFQELLLGFERTGLPFPAALKPPLGAATIEEALPEGFQERVGGRGVVHGGWVPQPSILCHPSVGCFVSHCGFGSIWESLMSDPQIVLREENGWVSKESLCKAIKSVMDEEIEVGCLVKKNHAEWKETPMSQGFMCNYIDNFVRQILDLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YJH4</accession>
    <name>A0AA38YJH4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Dynamin-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_025361</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YJH4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08771">
        <property type="entry name" value="DLP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001030">
        <property type="entry name" value="Dynamin-related protein 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022812">
        <property type="entry name" value="Dynamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001401">
        <property type="entry name" value="Dynamin_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030381">
        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566">
        <property type="entry name" value="DYNAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566:SF169">
        <property type="entry name" value="DYNAMIN-LIKE PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00195">
        <property type="entry name" value="DYNAMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00053">
        <property type="entry name" value="DYNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51718">
        <property type="entry name" value="G_DYNAMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynamin-type G" evidence="2">
        <location>
            <begin position="54"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="417"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="635"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="417"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="636"/>
            <end position="654"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9671614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="838" mass="93012" checksum="8BEDB71691F2872D" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YKT2</accession>
    <name>A0AA38YKT2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">glycerophosphodiester phosphodiesterase</fullName>
            <ecNumber evidence="2">3.1.4.46</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_025765</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a sn-glycero-3-phosphodiester + H2O = an alcohol + sn-glycerol 3-phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12969"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:83408"/>
            <dbReference type="EC" id="3.1.4.46"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycerophosphoryl diester phosphodiesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.46" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YKT2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008889">
        <property type="term" value="F:glycerophosphodiester phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006071">
        <property type="term" value="P:glycerol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08603">
        <property type="entry name" value="GDPD_SHV3_repeat_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08604">
        <property type="entry name" value="GDPD_SHV3_repeat_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.190:FF:000011">
        <property type="entry name" value="Glycerophosphodiester phosphodiesterase GDPDL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.190:FF:000013">
        <property type="entry name" value="Glycerophosphodiester phosphodiesterase GDPDL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43620:SF7">
        <property type="entry name" value="GLYCEROPHOSPHODIESTER PHOSPHODIESTERASE GDPD5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43620">
        <property type="entry name" value="GLYCEROPHOSPHORYL DIESTER PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03009">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0319">Glycerol metabolism</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041365131" description="glycerophosphodiester phosphodiesterase" evidence="9">
        <location>
            <begin position="22"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="domain" description="GP-PDE" evidence="10">
        <location>
            <begin position="39"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="GP-PDE" evidence="10">
        <location>
            <begin position="355"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="705"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="711"/>
            <end position="725"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9672282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="750" mass="82028" checksum="210E603E527592FF" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YLU8</accession>
    <name>A0AA38YLU8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Charged multivesicular body protein 2a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_026149</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YLU8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10476">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03357">
        <property type="entry name" value="Snf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="10"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9672800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="110" mass="12699" checksum="A4F878D7D5D90AEB" modified="2024-01-24" version="1">MGQMNRQMNLPSLQKIMQEFERQNEKMEMTMEVMGDAIDDALEGDEEEEETEELVSQVLNEIGIDIKSEVLFSHLYMFFPCPPELSSTVTAPGVNNRNNEIEFQIKNSCY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YM52</accession>
    <name>A0AA38YM52_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_026307</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YM52"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9672981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="120" mass="13087" checksum="1DDE3C55DFB07892" modified="2024-01-24" version="1">MAISWVNRVTRPALTCLAPPRPGVALGALGLEDLLSRSSITRRCISERRHLSDHNATSALEFSEMIAVRFQNFRSPSNLGKESGCGRDEMMRLSLGSLTLTALAARMRKRAYSLVGGVAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YNI7</accession>
    <name>A0AA38YNI7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_023355</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YNI7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000285">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000338">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000730">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000983">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24015:SF739">
        <property type="entry name" value="OS03G0644200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24015">
        <property type="entry name" value="OS07G0578800 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="147"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="247"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="348"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="449"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="519"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="550"/>
            <end position="584"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9673743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="614" mass="68942" checksum="13A8CF34F5263181" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YQE2</accession>
    <name>A0AA38YQE2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Elongation of fatty acids protein 3-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_023896</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YQE2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009922">
        <property type="term" value="F:fatty acid elongase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034625">
        <property type="term" value="P:fatty acid elongation, monounsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034626">
        <property type="term" value="P:fatty acid elongation, polyunsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019367">
        <property type="term" value="P:fatty acid elongation, saturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002076">
        <property type="entry name" value="ELO_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157:SF123">
        <property type="entry name" value="F25A4.4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157">
        <property type="entry name" value="FATTY ACID ACYL TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01151">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="29"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="65"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="121"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="174"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="207"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="238"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="273"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9674637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="301" mass="34968" checksum="C4B086AA30E894FD" modified="2024-01-24" version="1">MQSIKYWLSEHPCIVRFRWSHSQSWGSTWSFLFTSIAAYIATAAFLHLFLLLIRRRRPVPLGPIPALHSLAMALISVLIFVGILFSAAAEIRDTRWFWRRSKTPLQWLFCFPLGTRPSGRVFFWSYVFYLSRFLHLFRTYLKVLRRRRLAFFQVFNQSILLCTSYLWLEFSQSFQVLAILSMTLVYSMVYGYRFWTAIGLPSACPPFVVNCQMILLGCNLVWHFGVLFLHFLKGGCNGIGAWVFNSVLNAFILLLFLNFYVKMHLSKREDAPLVQPDSSSARHRTGSEMMGDRLTVKAKNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YRB9</accession>
    <name>A0AA38YRB9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GDSL esterase/lipase EXL3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_024145</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YRB9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1110:FF:000003">
        <property type="entry name" value="GDSL esterase/lipase APG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050592">
        <property type="entry name" value="GDSL_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45642">
        <property type="entry name" value="GDSL ESTERASE/LIPASE EXL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45642:SF95">
        <property type="entry name" value="GDSL-LIKE LIPASE_ACYLHYDROLASE FAMILY PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9675074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="364" mass="39784" checksum="A2D5ED64BF80720B" modified="2024-01-24" version="1">MVSLTMELWSSSSITVSFLSVFIILCATEALVKLPRNETFPAVLVFGDSIVDPGNNNNLKTLIKCNFPPYGRDFVGGFPTGRFSNGKIPPDFIAEELGIKQLLPSYSSLSLQLGDLLTGVSFASGGSGFDPLTPKLVSVLSLPDQLGMFKEYIGKLKVMVGEERTNTILSKSLFLVVAGSDDIANTYFVIGVRKRQYDVPAYTDFMATSAASFLKELYGLGARRIGVASAPPLGCLPSQRSLAGGKQRECAEDHNEAAKLFNTKLSSQLDSLNANFPQAKFVYIDIYKPFLDLIQNPQKSGFEVVDKGCCGSGRIEVAVLCNPLSPFTCENASNYVFWDSYHPTEEAYKVIIEEIIQKCVDSFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YRE1</accession>
    <name>A0AA38YRE1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_024256</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YRE1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08284">
        <property type="entry name" value="RVP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9675230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="115" mass="12848" checksum="F13550ECBAC4A0C1" modified="2024-01-24" version="1">MKIKGTIRSKEVIILVDSGATRNFLSLELVQQPGICRRVCISMQGLIVVEDFLPLELGNTDVILGMPWLGILRDVKVNWKMLTMKIRMGKTMIVLKGDSSLSRTEVSLKAMARAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YSM1</accession>
    <name>A0AA38YSM1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">TIR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_024692</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the disease resistance TIR-NB-LRR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YSM1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050832">
        <property type="term" value="P:defense response to fungus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043068">
        <property type="term" value="P:positive regulation of programmed cell death"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10140:FF:000007">
        <property type="entry name" value="Disease resistance protein (TIR-NBS-LRR class)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045344">
        <property type="entry name" value="C-JID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011713">
        <property type="entry name" value="Leu-rich_rpt_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058192">
        <property type="entry name" value="WHD_ROQ1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017:SF292">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20160">
        <property type="entry name" value="C-JID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07725">
        <property type="entry name" value="LRR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23282">
        <property type="entry name" value="WHD_ROQ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0611">Plant defense</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="TIR" evidence="10">
        <location>
            <begin position="17"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9675859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="800" mass="90548" checksum="8D4AABFDB1F20ADA" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YTU4</accession>
    <name>A0AA38YTU4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DEK-C domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_021877</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9676576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YTU4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000779">
        <property type="term" value="P:regulation of double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000220">
        <property type="entry name" value="DEK domain-containing chromatin associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044198">
        <property type="entry name" value="DEK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014876">
        <property type="entry name" value="DEK_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13468">
        <property type="entry name" value="DEK PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13468:SF1">
        <property type="entry name" value="PROTEIN DEK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08766">
        <property type="entry name" value="DEK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109715">
        <property type="entry name" value="DEK C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51998">
        <property type="entry name" value="DEK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="DEK-C" evidence="8">
        <location>
            <begin position="476"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="264"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="531"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="7"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="51"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="65"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="76"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="108"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="337"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="379"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="419"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="454"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="534"/>
            <end position="570"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9676576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="570" mass="63468" checksum="FE8D95203922C8CB" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YU16</accession>
    <name>A0AA38YU16_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">UDP-arabinopyranose mutase</fullName>
            <ecNumber evidence="4 9">5.4.99.30</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_021906</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Probable UDP-L-arabinose mutase involved in the biosynthesis of cell wall non-cellulosic polysaccharides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>UDP-beta-L-arabinofuranose = UDP-beta-L-arabinopyranose</text>
            <dbReference type="Rhea" id="RHEA:28350"/>
            <dbReference type="ChEBI" id="CHEBI:61457"/>
            <dbReference type="ChEBI" id="CHEBI:61463"/>
            <dbReference type="EC" id="5.4.99.30"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the RGP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.30" evidence="4 9"/>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9676624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YU16"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009505">
        <property type="term" value="C:plant-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052691">
        <property type="term" value="F:UDP-arabinopyranose mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071669">
        <property type="term" value="P:plant-type cell wall organization or biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033356">
        <property type="term" value="P:UDP-L-arabinose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004901">
        <property type="entry name" value="RGP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037595">
        <property type="entry name" value="RGP_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31682:SF45">
        <property type="entry name" value="UDP-ARABINOPYRANOSE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31682">
        <property type="entry name" value="UDP-ARABINOSE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03214">
        <property type="entry name" value="RGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016429">
        <property type="entry name" value="UPTG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6 11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="349"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="360"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="site" description="Required for activity" evidence="10">
        <location>
            <position position="152"/>
        </location>
    </feature>
    <feature type="site" description="Required for activity" evidence="10">
        <location>
            <position position="159"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (Glc...) arginine" evidence="11">
        <location>
            <position position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR016429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR016429-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR016429-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9676624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="370" mass="41930" checksum="B1567E4DA00C0E27" modified="2024-01-24" version="1">MAQPATPVTPALKDDLDIVIPTIRNLDFLEMWRPFFQPYHLIIVQDGDPTKTIRVPEGFDYELYNRNDVNRILGPKASCISFKDSACRCFGFLVSKKKYIFTIDDDCFVAKDPSGKEINALAQHIQNLLTPSTPFFFNTLYDPFREGADFVRGYPFSLREGAPTAISHGLWLNIPDYDAPTQLVKPLERNTRYVDAVMTIPKGTLFPMCGMNLGFDRELIGPAMYFGLMGDGQPIGRYDDMWAGWCAKVICDHMSLGVKTGLPYIWHSKASNPFVNLKKEYKGIYWQEEIIPFFQSVAFPKECITVQQCYIELSNQVKEKLGHVDPYFQKLADSMVTWIEAWEELNPPTKAAPAAQSNGVKKDAPAPKPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YUA1</accession>
    <name>A0AA38YUA1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Uridine kinase</fullName>
            <ecNumber evidence="12">2.7.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_021943</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>cytidine + ATP = CMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17562"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.48"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>uridine + ATP = UMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16825"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16704"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.48"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Pyrimidine metabolism; CTP biosynthesis via salvage pathway; CTP from cytidine: step 1/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 12">Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uridine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the uridine kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the UPRTase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the uridine kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.48" evidence="12"/>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9676667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YUA1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004849">
        <property type="term" value="F:uridine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000904">
        <property type="term" value="P:regulation of starch metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044206">
        <property type="term" value="P:UMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02023">
        <property type="entry name" value="UMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000015">
        <property type="entry name" value="Uridine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000339">
        <property type="entry name" value="Uridine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006083">
        <property type="entry name" value="PRK/URK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000764">
        <property type="entry name" value="Uridine_kinase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001097">
        <property type="entry name" value="PRK00129.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004018">
        <property type="entry name" value="PRK05480.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00235">
        <property type="entry name" value="udk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10285">
        <property type="entry name" value="URIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00485">
        <property type="entry name" value="PRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14681">
        <property type="entry name" value="UPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00988">
        <property type="entry name" value="URIDINKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="8 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribulokinase/uridine kinase" evidence="13">
        <location>
            <begin position="51"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="14">
        <location>
            <begin position="267"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF14681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9676667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="476" mass="53385" checksum="1DFFAA36A08B322E" modified="2024-01-24" version="1">MDNAMETASGAHFYGLRPDGLLSCSASSPRSHSSSLSVPSGDHSVPKQPFVIGVTGGTASGKTTVCDMIIQQLHDHRVVLVNQDSFYRGLTAKELEYVHEYNFDHPDAFDTEQLLDCILKLKSGQSVHVPIYDFKTHQRRSDSFRQVNASDVIILEGILVFHDQCVRDLMNMKIFVDADADVRLARRISRDTVERGRDIQSVLQQYAKFVKPAFDNFILPSKKFADVIIPRGAENHVAIDLIVQHIRTKLGQHDLCKRYPNVNVIHSTFQIRGMHTLIRDREISKHDFVFYSDRLIRLVVEHGLGYLPFIEKQVVTPTGSVYAGVEFCKKLCGVSIIRSGESMENALRACCKGIKIGKILIHRDGDNGRQLIYEKLPKDISVRHVLLLDPVLATGNSANQAIELLIKKGVPESSIIFLNLISAPEGIHCVCNRFPSLKIVTSEIDAKLNEDFRVIPGLGEFGDRYFGTDDYFVPIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YUT5</accession>
    <name>A0AA38YUT5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Transcription factor CYCLOIDEA-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_022200</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YUT5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000032">
        <property type="term" value="P:regulation of secondary shoot formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017888">
        <property type="entry name" value="CYC/TB1_R_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017887">
        <property type="entry name" value="TF_TCP_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005333">
        <property type="entry name" value="Transcription_factor_TCP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31072:SF226">
        <property type="entry name" value="TRANSCRIPTION FACTOR TCP18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31072">
        <property type="entry name" value="TRANSCRIPTION FACTOR TCP4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03634">
        <property type="entry name" value="TCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51370">
        <property type="entry name" value="R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51369">
        <property type="entry name" value="TCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TCP" evidence="8">
        <location>
            <begin position="138"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="R" evidence="9">
        <location>
            <begin position="265"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="122"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="226"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="135"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="239"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="265"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9677085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="401" mass="45193" checksum="4C57AFA1A962CFC4" modified="2024-01-24" version="1">MEIGIHANSLACAPLGCPGAIFQPQMFLSNSNGNNPISYPDQTILHAPFITDVSYNSQQEDPPSSFFYFPSPFIHYEDDAFLQHNDLIPQLQALLPDDTVADTMVNMVNSNKNCNELIGDRKQSNSVTEPIMQKRSSKRDRHSKINTARGPRDRRMRLSLEIARKFFDLQDMLGFDKASKTVEWLLIKAKSAIKELSRGVPQMKHSCSISVKSASSTSDCEELSGIDTINGDQQGSISKGKRSKCLTKERKTRQSRKNAFQPLARESREKARARARERTREKMWSRRLDESKVCAEANNRDLNHLGSISSFETGEESGSQSHNLIPSLELLPEVEEPSSNEQHHSGITEAMLDNSLVIMSKQWSPSSIFNYLNFGISQEPQFPGFQFHGKPWEAHSNENLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YUY1</accession>
    <name>A0AA38YUY1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_022138</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9676993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YUY1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000162">
        <property type="entry name" value="Adenine nucleotide alpha hydrolase-like domain kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001543">
        <property type="entry name" value="Cysteine-rich receptor-like protein kinase 41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052059">
        <property type="entry name" value="CR_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47973">
        <property type="entry name" value="CYSTEINE-RICH RECEPTOR-LIKE PROTEIN KINASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="145"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9676993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="483" mass="53580" checksum="069C98849C35B1F4" modified="2024-01-24" version="1">MKENIKLKCSESGRKFRKAELTRNSHGELKPSQGCAVQVEIRGKGNHTAFHLKLSFHTVIFNTKLKVRRRCVSGSRYSRRFHQFFGLNLPIDASMGFLSLLCSCLERRLKGRGGAGEDEADAADDGDSFDLFFDLRALQVATNFFSEFNELGHGGFGPVFKGLMPNGTQIAVKKLSLDSRQGVREFTNEVKLLLKIQHKNLVTLLGCCVEGPEKMLVYEYLPNKSLDCFLFDKTKSASLDWATRFRIVMGVVRGLLYLHEEAPERIIHRDIKASNILLDEKLNPKISDFGLARLFPGDETHVNTFRISGTHGYMAPEYALHGYLSVKTDVFSFGVLVLEIVSGRKNHNSHLSAEMTDLLSYTWKLYQGGKALELVDPSLAKCNPDEAAMCIQLGLLCCQASVADRPDMNSVHLMLSSDSFNLPRPGKPGIHGRTGRWTTTTSAFTNTNATSTSTGVTKASAGSSFVEEYSRNSISISSLDEGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YVI9</accession>
    <name>A0AA38YVI9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Glucose/Sorbosone dehydrogenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_022345</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyrroloquinoline quinone</name>
            <dbReference type="ChEBI" id="CHEBI:58442"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the PQQ oxidoreductase GdhB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YVI9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000067">
        <property type="entry name" value="HHIP-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012938">
        <property type="entry name" value="Glc/Sorbosone_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011041">
        <property type="entry name" value="Quinoprot_gluc/sorb_DH_b-prop"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19328">
        <property type="entry name" value="HEDGEHOG-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19328:SF13">
        <property type="entry name" value="HIPL1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07995">
        <property type="entry name" value="GSDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50952">
        <property type="entry name" value="Soluble quinoprotein glucose dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0634">PQQ</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041462472" description="Glucose/Sorbosone dehydrogenase domain-containing protein" evidence="11">
        <location>
            <begin position="25"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose/Sorbosone dehydrogenase" evidence="12">
        <location>
            <begin position="220"/>
            <end position="573"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00061483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF07995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9677292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="695" mass="75459" checksum="D4049A70578D762E" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YXT1</accession>
    <name>A0AA38YXT1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">RecF/RecN/SMC N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_020777</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SMC family. SMC6 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YXT1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030915">
        <property type="term" value="C:Smc5-Smc6 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051276">
        <property type="term" value="P:chromosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036277">
        <property type="entry name" value="SMC_hinge_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19306">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES 5,6 SMC5, SMC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19306:SF6">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75553">
        <property type="entry name" value="Smc hinge domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RecF/RecN/SMC N-terminal" evidence="13">
        <location>
            <begin position="22"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="255"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="328"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="611"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="676"/>
            <end position="788"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9678693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1026" mass="118133" checksum="EFD53C82452EFF39" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YY57</accession>
    <name>A0AA38YY57_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_020865</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YY57"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000213">
        <property type="entry name" value="Tyrosine-sulfated glycopeptide receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046956">
        <property type="entry name" value="RLP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48061:SF12">
        <property type="entry name" value="DISEASE RESISTANCE LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48061">
        <property type="entry name" value="LEUCINE-RICH REPEAT RECEPTOR PROTEIN KINASE EMS1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="13">
        <location>
            <begin position="15"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="850"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="855"/>
            <end position="871"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9678799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="927" mass="102877" checksum="DE01644101D58C85" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z003</accession>
    <name>A0AA38Z003_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CAAX prenyl protease 2/Lysostaphin resistance protein A-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_021518</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z003"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004175">
        <property type="term" value="F:endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080120">
        <property type="term" value="P:CAAX-box protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003675">
        <property type="entry name" value="Rce1/LyrA-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43592">
        <property type="entry name" value="CAAX AMINO TERMINAL PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43592:SF18">
        <property type="entry name" value="CAAX AMINO TERMINAL PROTEASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02517">
        <property type="entry name" value="Rce1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="193"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="282"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="314"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="337"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="CAAX prenyl protease 2/Lysostaphin resistance protein A-like" evidence="2">
        <location>
            <begin position="284"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9679617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="378" mass="42721" checksum="6EF147AC999EB5A6" modified="2024-01-24" version="1">MQRARVFPSIAFLHLNPPSTDFGQSDAATMSAPCCRTLSHYPSLISPPISRIRISNLARTSLSLKQRFRIVDSFSRYSKNKWRVSCHREEESSAEIPEFETVEDKLPEELANPEVEQSDNLKKDWVSNIRKALKAADAFFGMKPWTVPWTAMTILQVMLLWIASFWLVGYWIIPFLAHKAGFRKESMTHRGQALYGLLGEVTEGLSGIAILHICLARFHPLPSDWFRLSLEGKWYFDVGLGCLMFPLVNRLSQINENLLPVAPSTPVTGSSVQQSIVARDPVAMALYAVFLSVCAPIWEEIFFRGFLLPSLTRYMPVWCSILVSSVAFASAHLNLQTMLPFIFLGMLIGAVFARSRNLLASMLLHSLWNAFVFVDLMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z077</accession>
    <name>A0AA38Z077_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Homeobox-leucine zipper protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">HD-ZIP protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Homeodomain transcription factor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_021754</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Probable transcription activator that may act as growth regulators in response to water deficit.</text>
    </comment>
    <comment type="function">
        <text evidence="11">Transcription factor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 11">Belongs to the HD-ZIP homeobox family. Class I subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z077"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009737">
        <property type="term" value="P:response to abscisic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009414">
        <property type="term" value="P:response to water deprivation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000293">
        <property type="entry name" value="Homeobox-leucine zipper protein ATHB-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045224">
        <property type="entry name" value="HDZip_class_I_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000047">
        <property type="entry name" value="HTH_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003106">
        <property type="entry name" value="Leu_zip_homeo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24326">
        <property type="entry name" value="HOMEOBOX-LEUCINE ZIPPER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24326:SF122">
        <property type="entry name" value="HOMEOBOX-LEUCINE ZIPPER PROTEIN HOX6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02183">
        <property type="entry name" value="HALZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00031">
        <property type="entry name" value="HTHREPRESSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 9 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 11" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="14">
        <location>
            <begin position="19"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="21"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="121"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="78"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00058361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU369038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9679953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="224" mass="24972" checksum="D51BF824B1ACCDCB" modified="2024-01-24" version="1">MESRGCSAEAGEEGEQLSRKKSRNKKRFSDEQVKYLESIFESDSKLEPRKKEELAMELGMQPRQVAIWFQNKRARWKSKQIEQDYKALRASYDALTSRFESLKEEKQSLLAQLQKLGDLLEKPGDGGGSGFGGNSSTDGGSDTGDEAKLSYLEGGLDHRLVKCSDDDKSRSGGYFGHQGGPELLDKDENADISLESTGKWFGFASGGFHDQSCTISQLWDFWSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z1L1</accession>
    <name>A0AA38Z1L1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Mannosyltransferase</fullName>
            <ecNumber evidence="12">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_019755</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Mannosyltransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. In the lumen of the endoplasmic reticulum, adds the eighth mannose residue in an alpha-1,6 linkage onto Man(7)GlcNAc(2)-PP-dolichol to produce Man(8)GlcNAc(2)-PP-dolichol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>an alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate = an alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29535"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="Rhea" id="RHEA-COMP:19518"/>
            <dbReference type="Rhea" id="RHEA-COMP:19519"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:132517"/>
            <dbReference type="ChEBI" id="CHEBI:132519"/>
            <dbReference type="EC" id="2.4.1.260"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:29536"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Endoplasmic reticulum membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the glycosyltransferase 22 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="12"/>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z1L1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052917">
        <property type="term" value="F:dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005599">
        <property type="entry name" value="GPI_mannosylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760:SF1">
        <property type="entry name" value="DOL-P-MAN:MAN(7)GLCNAC(2)-PP-DOL ALPHA-1,6-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03901">
        <property type="entry name" value="Glyco_transf_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="94"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="127"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="169"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="213"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="261"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="290"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="313"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="346"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9680522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="496" mass="56856" checksum="3E08595E5747069B" modified="2024-01-24" version="1">MAPQSSKFLQNYGFDLVLGSIAAFYVFMAPYTKVEESFNVQAMHDILYHQHHLENYDHLEFPGVVPRTFIGALIVSILASPVILAMNLLHLPKIYSLFAVRLVLGCIILVTLRFFRIQVRNKFGHQVEAFFVILTALQFHMLFYCTRPLPNILSLGLVNLAYGYSLKGSFYAALKCLIFATIVFRCDMLLLLGPLGLQLLLTKSISLWESLKCGISTALLCIGLTTLVDTIMWKKLVWPEFEVLWFNSVLNRSSEWGTHSFHWYFTSALPRALLAAYPLCMLGVLLDRRILSYILPVFLFVLLYSKLPHKELRFIISSVPIFNLSAAIAANRIYNNRKKSLWKLLYFFLLGLLLISLGCTIIIFMSSYNNYPSGYGLKYLHQIGHSTNNNDEQWVHIDPFSAMNGISRFCENASPWRYSKEEGILIEEFRWRNFTFLINEHSNIEGFKCLFAVNGFSKIHLQSGFPPISLVEEPKVFILGNIKSEYVINRNWPGCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z1X0</accession>
    <name>A0AA38Z1X0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_019996</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z1X0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001023">
        <property type="entry name" value="Os07g0541700 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044576">
        <property type="entry name" value="At4g25390-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46821:SF2">
        <property type="entry name" value="OS03G0251700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46821">
        <property type="entry name" value="OS07G0586332 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="23"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="76"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="320"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="378"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="497"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="339"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9680851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="711" mass="79514" checksum="4180D8A012FB2A49" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z6L3</accession>
    <name>A0AA38Z6L3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Molybdate transporter 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_018716</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z6L3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015098">
        <property type="term" value="F:molybdate ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031563">
        <property type="entry name" value="MOT1/MOT2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31970:SF9">
        <property type="entry name" value="MOLYBDATE TRANSPORTER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16983">
        <property type="entry name" value="MFS_MOT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="153"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="193"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9682844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="244" mass="27112" checksum="6F374872D1F6EE27" modified="2024-01-24" version="1">MADDTPSTITTTPLLRPSRWWFSSLRLKTTLSAELGGSVGDLGTYIPIVLTLTLVNHLDLSTTLIFMPLYNISTGFLFGIPMPVQPMKSIAVVAISDPLLSLPQITAAGLSIAATLFIFGTTSLMSLLYRFISLPRRPWRLFRWKIRPPRNWLGLDEFGFALFSILFLVLVTSSGDFGDEVIPLGVHEEENDFVNHSCRPALIVFILGLVLCFIRDPSIVKDLRFGPSRIHLREDGKLGSSSDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z8K6</accession>
    <name>A0AA38Z8K6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7851 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_016636</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z8K6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057173">
        <property type="entry name" value="DUF7851"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36375">
        <property type="entry name" value="OS05G0459300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36375:SF1">
        <property type="entry name" value="OS05G0459300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25236">
        <property type="entry name" value="DUF7851"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7851" evidence="2">
        <location>
            <begin position="25"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9684245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="224" mass="25428" checksum="4F68F1E2A9F0CE01" modified="2024-01-24" version="1">MEEKEKSKKKKQKHQHPNDQTTKPSSDFAFKLSSEVKGLRFGGQFIVKSFTIRRARPLELLKVLSFPPINHHKDKTKAKLPFPSTTAFLPTNFTILAHHAWHTLTLGLGTKKSKVLLFVFESESLKVAVDRVWPPEIPLGEVNKKLIRGLTGYEMARFKFRKGCITFYVYAVRRAGNLGFSCADDLRTILQSVVELKDFLDHTAMLAMPHQRSIAFAPPVAMAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZA10</accession>
    <name>A0AA38ZA10_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Disease resistance RPP13-like protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_016963</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14798">
        <property type="entry name" value="RX-CC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057135">
        <property type="entry name" value="At4g27190-like_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056789">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038005">
        <property type="entry name" value="RX-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766:SF51">
        <property type="entry name" value="DISEASE RESISTANCE RPP13-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766">
        <property type="entry name" value="PLANT BROAD-SPECTRUM MILDEW RESISTANCE PROTEIN RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25019">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23247">
        <property type="entry name" value="LRR_RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0611">Plant defense</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="7">
        <location>
            <begin position="8"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="6">
        <location>
            <begin position="187"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="9">
        <location>
            <begin position="438"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="domain" description="R13L1/DRL21-like LRR repeat region" evidence="10">
        <location>
            <begin position="702"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein At4g27190-like leucine-rich repeats" evidence="8">
        <location>
            <begin position="1142"/>
            <end position="1269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF25019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9684748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1418" mass="161206" checksum="02D1DF2AAACC1EF8" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZB03</accession>
    <name>A0AA38ZB03_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Disease resistance protein RGA3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_017651</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZB03"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14798">
        <property type="entry name" value="RX-CC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038005">
        <property type="entry name" value="RX-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766:SF40">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766">
        <property type="entry name" value="PLANT BROAD-SPECTRUM MILDEW RESISTANCE PROTEIN RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="6">
        <location>
            <begin position="11"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="5">
        <location>
            <begin position="163"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="7">
        <location>
            <begin position="409"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9685696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="613" mass="70902" checksum="49B45000ACD1B025" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZDX5</accession>
    <name>A0AA38ZDX5_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_015584</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZDX5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27006:SF606">
        <property type="entry name" value="INTERLEUKIN-1 RECEPTOR-ASSOCIATED KINASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27006">
        <property type="entry name" value="PROMASTIGOTE SURFACE ANTIGEN PROTEIN PSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9686802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="89" mass="9713" checksum="F2EA9D0E5016CD30" modified="2024-01-24" version="1">MDQTLSETCNTNEFWRCVNARLLCVQEDPSDRHTMAIAVHLLSSDSTTMPVPKEPAFVVKGDLSNTASSSSKAEASWKNELLASIGEGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZFX6</accession>
    <name>A0AA38ZFX6_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_016176</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZFX6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9687584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="57" mass="6259" checksum="ACCAD542DFB0249F" modified="2024-01-24" version="1">MVNQLSFLKVQTILHFARESSSPTYSEAMKIPKKDPTIAPCHPSGLPSFCKLQAGNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZG27</accession>
    <name>A0AA38ZG27_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">NB-ARC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_016230</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZG27"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057135">
        <property type="entry name" value="At4g27190-like_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050905">
        <property type="entry name" value="Plant_NBS-LRR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463:SF209">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RPS2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23247">
        <property type="entry name" value="LRR_RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NB-ARC" evidence="5">
        <location>
            <begin position="4"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein At4g27190-like leucine-rich repeats" evidence="6">
        <location>
            <begin position="620"/>
            <end position="744"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9687659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="762" mass="86767" checksum="85CFE043E275D3D1" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZJK5</accession>
    <name>A0AA38ZJK5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Calcium-transporting ATPase</fullName>
            <ecNumber evidence="9">7.2.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_012612</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the hydrolysis of ATP coupled with the transport of calcium.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>Ca(2+)(in) + ATP + H2O = Ca(2+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18105"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.10" evidence="9"/>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZJK5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005388">
        <property type="term" value="F:P-type calcium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000125">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000205">
        <property type="entry name" value="Ca2+-transporting ATPase-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006408">
        <property type="entry name" value="P-type_ATPase_IIB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01517">
        <property type="entry name" value="ATPase-IIB_Ca"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093:SF470">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE 12, PLASMA MEMBRANE-TYPE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093">
        <property type="entry name" value="CATION TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="7 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="231"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="296"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="690"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="714"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="826"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="857"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="10">
        <location>
            <begin position="114"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase C-terminal" evidence="11">
        <location>
            <begin position="710"/>
            <end position="879"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9690035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="893" mass="99066" checksum="852226ABAE8B18FE" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZK78</accession>
    <name>A0AA38ZK78_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CTLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_012821</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZK78"/>
    <dbReference type="SMR" id="A0AA38ZK78"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013144">
        <property type="entry name" value="CRA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024964">
        <property type="entry name" value="CTLH/CRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006595">
        <property type="entry name" value="CTLH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050618">
        <property type="entry name" value="Ubq-SigPath_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12864">
        <property type="entry name" value="RAN BINDING PROTEIN 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10607">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08513">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00757">
        <property type="entry name" value="CRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00668">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50897">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CTLH" evidence="1">
        <location>
            <begin position="77"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9690364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="242" mass="27566" checksum="CA29F5AB48CD1A95" modified="2024-01-24" version="1">MSLFCIVIRELEEIESMASSKKVITREEWERKLNDVKIRKEDMNKLVMNFLVTEGYVDAAEKFRMESGTEPDIDLATITDRMAVKKAVQCGNVEDAIEKVNDLNPEILDTNPQLFFHLQQQRLIELIRNGKVDEALEFAQEELAPRGEENQSFLEELEKTVALLAFEDFSNCPVGELLDISQRLKTASEVNAAILTSQSHEKDPKLPSLLKMLIWAQNQLDDKAAYPRINDLSTARLEDPAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZKN1</accession>
    <name>A0AA38ZKN1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Apoptosis inhibitor 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_012960</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the API5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZKN1"/>
    <dbReference type="SMR" id="A0AA38ZKN1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043067">
        <property type="term" value="P:regulation of programmed cell death"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008383">
        <property type="entry name" value="API5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12758:SF19">
        <property type="entry name" value="APOPTOSIS INHIBITOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12758">
        <property type="entry name" value="APOPTOSIS INHIBITOR 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05918">
        <property type="entry name" value="API5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="474"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="493"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="526"/>
            <end position="543"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9690575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="550" mass="61477" checksum="240156C645E8AB2B" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZMK5</accession>
    <name>A0AA38ZMK5_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_011127</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DRM1/ARP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9691863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZMK5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008406">
        <property type="entry name" value="DRM/ARP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33565">
        <property type="entry name" value="DORMANCY-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33565:SF20">
        <property type="entry name" value="DORMANCY-ASSOCIATED PROTEIN HOMOLOG 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05564">
        <property type="entry name" value="Auxin_repressed"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="60"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="60"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9691863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="135" mass="14796" checksum="A3A2DD7F498BF2E5" modified="2024-01-24" version="1">MGFLDKLWDETVAGPPPETGLGKLRKYKSLSAARSPPILNPDEVPVTRTITILKTNSSFRNFSPHSVSVPNSPAGSSAPESPITPGTPTGDYKTDARRKAALEAFERAEPRSPTVYDWFSLNIFFTTLSDPQLKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZNQ8</accession>
    <name>A0AA38ZNQ8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">holo-[acyl-carrier-protein] synthase</fullName>
            <ecNumber evidence="2">2.7.8.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_011189</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.7" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9691962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZNQ8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008897">
        <property type="term" value="F:holo-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.470.20:FF:000011">
        <property type="entry name" value="Os08g0243100 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.20">
        <property type="entry name" value="4'-phosphopantetheinyl transferase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008278">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037143">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050559">
        <property type="entry name" value="P-Pant_transferase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004568">
        <property type="entry name" value="Ppantetheine-prot_Trfase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00556">
        <property type="entry name" value="pantethn_trn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12215:SF15">
        <property type="entry name" value="4'-PHOSPHOPANTETHEINYL TRANSFERASE SUPERFAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12215">
        <property type="entry name" value="PHOSPHOPANTETHEINE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01648">
        <property type="entry name" value="ACPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56214">
        <property type="entry name" value="4'-phosphopantetheinyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="4'-phosphopantetheinyl transferase" evidence="6">
        <location>
            <begin position="9"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9691962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="180" mass="20023" checksum="ADEBAFDA0976D6C2" modified="2024-01-24" version="1">MLDSLFSLQIGIDVEEKQRTTKNNILSFARRYFSPHEVESLSVISDPEIQRQEFLKLWTLKEAYVKALGRGFSAAPFKNFTIRFRAATKGHLHLSGNSNSKASEIIVESSDDPKTLTSNWQFALLELAGSHYAAICKEKDDDTVEGKGSVPMKLTVRKTIPFVEDECISGKDALVLVGGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZNV5</accession>
    <name>A0AA38ZNV5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_011235</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZNV5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099402">
        <property type="term" value="P:plant organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000158">
        <property type="entry name" value="pentatricopeptide repeat-containing protein At2g33680"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF537">
        <property type="entry name" value="PENTACOTRIPEPTIDE-REPEAT REGION OF PRORP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9692037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="345" mass="38718" checksum="0E393DE0F165CEC3" modified="2024-01-24" version="1">MDHARFILDQTPSPTDFSWNSLIRAYTLHDSPQNSLAKPSNVTYPFVLKSCSNLSSVLEGEHIHTHVLRLGFGSDLFCFCLDSSRILWVDMGVGDEVSWNSIISGYLFEEMPMRRNAVCRTAMINVYGKERDFVEMLSLFSGCFNLGEKFICTLNGLELNVILATALVDMYAKCGKIDDACLVFVKTSEKDVVALWNAMILGLAYHGDGSDSLAPNDVAFIGGLSACNHSGLVEEGRVQFSSMADKHGLSPKLEHCACMVDLLGRAGHLKEAYELVQNMDPNFCILLSNFYVSAGRWKDVARVRRQVKAKRIEKDRKAFWLFVVEDTTHLKSQEIYSTYEILIDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZP84</accession>
    <name>A0AA38ZP84_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA/RNA-binding protein Alba-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_011616</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZP84"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.110.20:FF:000005">
        <property type="entry name" value="Uncharacterized protein At2g34160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.20">
        <property type="entry name" value="Alba-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036882">
        <property type="entry name" value="Alba-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002775">
        <property type="entry name" value="DNA/RNA-bd_Alba-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014560">
        <property type="entry name" value="UCP030333_Alba"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31947:SF43">
        <property type="entry name" value="ALBA DNA_RNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31947">
        <property type="entry name" value="DNA/RNA-BINDING PROTEIN ALBA 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01918">
        <property type="entry name" value="Alba"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF030333">
        <property type="entry name" value="UCP030333_Alba"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82704">
        <property type="entry name" value="AlbA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA/RNA-binding protein Alba-like" evidence="1">
        <location>
            <begin position="20"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9692627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="130" mass="14420" checksum="21F8CF069BBD056D" modified="2024-01-24" version="1">MEEITEAVNNINISDSHKKNRIQVSNTKKPLFFYVNLAKRYMQQHNEVELSALGMAIATVVTIAEILKNNGLAVEKKITTSTVDMKDESRGRPVQKAKIEILLGKTANFDELMAAAAEEREAGGDVEEQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZR06</accession>
    <name>A0AA38ZR06_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cyclin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_009578</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFIIB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9693690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZR06"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000126">
        <property type="term" value="C:transcription factor TFIIIB complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000995">
        <property type="term" value="F:RNA polymerase III general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001006">
        <property type="term" value="F:RNA polymerase III type 3 promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070897">
        <property type="term" value="P:transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20553">
        <property type="entry name" value="CYCLIN_TFIIIB90_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20554">
        <property type="entry name" value="CYCLIN_TFIIIB90_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.650:FF:000002">
        <property type="entry name" value="Cyclin/Brf1-like TBP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000007">
        <property type="entry name" value="Transcription factor IIIB 90 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000066">
        <property type="entry name" value="Transcription factor IIIB subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.650">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011665">
        <property type="entry name" value="BRF1_TBP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000812">
        <property type="entry name" value="TFIIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013150">
        <property type="entry name" value="TFIIB_cyclin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618:SF4">
        <property type="entry name" value="TRANSCRIPTION FACTOR IIIB 90 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07741">
        <property type="entry name" value="BRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00382">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00685">
        <property type="entry name" value="TIFACTORIIB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Cyclin-like" evidence="11">
        <location>
            <begin position="76"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="11">
        <location>
            <begin position="177"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="332"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="494"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="545"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="342"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="358"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="554"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="580"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="610"/>
            <end position="622"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9693690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="622" mass="68082" checksum="3B473A6F6CF9A6E6" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZRB4</accession>
    <name>A0AA38ZRB4_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_011888</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NRAMP (TC 2.A.55) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZRB4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015086">
        <property type="term" value="F:cadmium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034755">
        <property type="term" value="P:iron ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00221">
        <property type="entry name" value="NRAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001046">
        <property type="entry name" value="NRAMP_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01197">
        <property type="entry name" value="nramp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037982">
        <property type="entry name" value="Nramp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001923">
        <property type="entry name" value="PRK00701.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11706:SF54">
        <property type="entry name" value="METAL TRANSPORTER NRAMP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11706">
        <property type="entry name" value="SOLUTE CARRIER PROTEIN FAMILY 11 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01566">
        <property type="entry name" value="Nramp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00447">
        <property type="entry name" value="NATRESASSCMP"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="44"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="79"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="121"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="152"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="183"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="221"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="269"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="327"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="366"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="391"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="423"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="467"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9692969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="541" mass="58639" checksum="6D95654B0FDF919F" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZRX6</accession>
    <name>A0AA38ZRX6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_009929</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZRX6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000413">
        <property type="entry name" value="Inactive leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000077">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000267">
        <property type="entry name" value="probable LRR receptor-like serine/threonine-protein kinase IRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000295">
        <property type="entry name" value="probable LRR receptor-like serine/threonine-protein kinase IRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000402">
        <property type="entry name" value="Putative LRR receptor-like serine/threonine-protein kinase IRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050994">
        <property type="entry name" value="At_inactive_RLKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48010">
        <property type="entry name" value="OS05G0588300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48010:SF19">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="632"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="711"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="740"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9694185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="993" mass="107753" checksum="8309BF3D946BDAE6" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZS87</accession>
    <name>A0AA38ZS87_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase Ty1/copia-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_012255</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9693403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZS87"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439">
        <property type="entry name" value="GAG-POL-RELATED RETROTRANSPOSON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439:SF502">
        <property type="entry name" value="SECRETED RXLR EFFECTOR PROTEIN 161-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase Ty1/copia-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9693403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="315" mass="35121" checksum="5DC8DCE78C97A6CF" modified="2024-01-24" version="1">MLVTGSNVRLLAEFKMEMQDVFEMSNLGIMNYFLGMEIYQCSSGIFISQRKYAVDILKKFKLESCKEVATPLAQNEKISKNDGEKLEEPSTYRSLVGSLLYLTTTRPDLMFPAGLLSRFMSSPSNVHMGVAKRVLKYVRGTTNLGIWYLKTGGVKLDGYADSDWVGSVDDMKSTSGYVFTIGSGVICWNSRKQEVVAQSTAEAEYISLVAAANQGIWLRKLLADLGQEQSSPTELCCDNKSAIAIAQNPVQHGRTKHINVKFHSVREAEKNSLVKLHYCSTDVQLADIMTKALPKSRLEFLRLKLGMSKANLKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZVN3</accession>
    <name>A0AA38ZVN3_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_008350</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZVN3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9696047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="216" mass="24519" checksum="423D96E01C88596C" modified="2024-01-24" version="1">MEKHSCELPNNGESDNQESDLDQKCVSKDLKGSKLKQRIMLHEVEPLALQALHKLVALVHDSVKVADRTEPVHSTSGNDSDVKVKSEQKRIVASNLFRLEDDPLAKILWTLEPLTLQNVFLAMVHNFPRTLEALILHLLSPVGAEVLTRKFEEMDQHITEEERNEFYQLFYGVFDDQFAAMKAILNGKESFAFKAFKNVLDQYLGVNYDGPKPLVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZWX7</accession>
    <name>A0AA38ZWX7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PVL29_008658</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 20">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the RING-type zinc finger family. ATL subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZWX7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16461">
        <property type="entry name" value="RING-H2_EL5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000187">
        <property type="entry name" value="E3 ubiquitin-protein ligase ATL6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044600">
        <property type="entry name" value="ATL1/ATL16-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46913">
        <property type="entry name" value="RING-H2 FINGER PROTEIN ATL16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46913:SF1">
        <property type="entry name" value="RING-H2 FINGER PROTEIN ATL16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="24"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="17">
        <location>
            <begin position="109"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="174"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="193"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ9696544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="263" mass="28741" checksum="529096A816D699C8" modified="2024-01-24" version="1">MDDKTSGISKGCAPSSYALSGKVMLSSVVILFTVVAIIVFIHSYGRWLLRRDRTRRHLRRRGRIRFDSDRTTAVSTVHGVGDQGLDVSILKSLPTFVYSKATHGPILECAVCLSEFEDDEKGRVLPKCNHCFHNDCIDTWFHSHSNCPLCRALVPLHIPSPAETVVPVLEPAGTETISGASPRCPRDDDENETGPSSSSTPGTLTLLECPRKPLELVGITVEMPAMRAGGCRGLEEVELGTTENQSSKSPGSRILSLKRIWST</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZY56</accession>
    <name>A0AA38ZY56_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Retrotransposon Copia-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_009147</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZY56"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9697234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="75" mass="8370" checksum="450CE962ECD5E53D" modified="2024-01-24" version="1">MATKNPIFTSVISGSPTITLEKLIGSENYLSWSASAELWFMGQGYEDYLVTPEDVIPDVDKVQWKKIDAQLCSVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZY95</accession>
    <name>A0AA38ZY95_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_009297</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZY95"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000294">
        <property type="entry name" value="Serine/threonine-protein kinase OXI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000312">
        <property type="entry name" value="Serine/threonine-protein kinase OXI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45637">
        <property type="entry name" value="FLIPPASE KINASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="18"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="186"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="385"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="199"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="223"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="400"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9697412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="426" mass="48230" checksum="1F655D6541F3CA5E" modified="2024-01-24" version="1">MIDHHHHGDVPMLDLPCLKALAALGRGAKGAVFLVEAEAEAGEGRLALKAVSRKLVESKSKDNDKNGSNNDKESDYRRICFEQEALRSFRHPLLPRLHGIVCTDMIVGYAIDYCPGRDLNQLRKKQSEKMFSDDIIRFYAAELVLALEYLHGLGIVYRDLKPENVLIQENGHLMLVDFDLSTKLSPKSPENPPPILKKPAQKPAKKTRLSSFHRLRNSGISSEKSDPALESERNSVRTESDSSAKSNSFVGTEEYVAPEIILGSGHDFSVDWWSLGVMLYEMLYGTTPFKGSNRKETFYRILAKSPELVGEATPLRDLIAKLLEKDPKQRIILEDIKSHDFFRGIEWDMVMEIGRPPYIPENEHVEGRFQDLDIESLVHEAFKIGDGDDNKKNSNNNNNNKEESEKKGVWVEGLNHKSEINDFLIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZYV3</accession>
    <name>A0AA38ZYV3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">BED-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_009244</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZYV3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007021">
        <property type="entry name" value="DUF659"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008906">
        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003656">
        <property type="entry name" value="Znf_BED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32166:SF74">
        <property type="entry name" value="OS05G0256350 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32166">
        <property type="entry name" value="OSJNBA0013A04.12 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04937">
        <property type="entry name" value="DUF659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50808">
        <property type="entry name" value="ZF_BED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BED-type" evidence="9">
        <location>
            <begin position="9"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="106"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="673"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="126"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="673"/>
            <end position="688"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9697347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="752" mass="86871" checksum="937D5F66B6651B46" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZZ80</accession>
    <name>A0AA38ZZ80_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cyclin-D-binding Myb-like transcription factor 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_007244</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZZ80"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47430">
        <property type="entry name" value="GB|AAC33480.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47430:SF4">
        <property type="entry name" value="GB|AAC33480.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="4">
        <location>
            <begin position="477"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="5">
        <location>
            <begin position="482"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="4">
        <location>
            <begin position="527"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="4">
        <location>
            <begin position="597"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="98"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="142"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="366"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="13"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="51"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="142"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="159"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="212"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="238"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="256"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="375"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9698043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="690" mass="79905" checksum="07DB6E06F8ADBD9C" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A0L1</accession>
    <name>A0AA39A0L1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UspA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_007565</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A0L1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23659">
        <property type="entry name" value="USP_At3g01520-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000206">
        <property type="entry name" value="Universal stress protein family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46100">
        <property type="entry name" value="IMP2'P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46100:SF7">
        <property type="entry name" value="UNIVERSAL STRESS PROTEIN PHOS34-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="1">
        <location>
            <begin position="5"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9698554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="164" mass="18043" checksum="BB76CD2C6400981E" modified="2024-01-24" version="1">MAKDRKIGVAVDFSQGSNIALKWAIDNLLDKGDTLFFIHVKPSQGDESRNLLWSATGSPLIPLEEFRDLDVAQKYEINLDPEFLGMLATASSQKKVKIIAKIYWGDARDKLCDAVAELKLDSLVMGSRGLGTIQRTFLGSVTNYVMVHATCPVTIVKDPSSHGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A1X0</accession>
    <name>A0AA39A1X0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">MBD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_007483</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A1X0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01396">
        <property type="entry name" value="MeCP2_MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.890.10">
        <property type="entry name" value="Methyl-cpg-binding Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001739">
        <property type="entry name" value="Methyl_CpG_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47941">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01429">
        <property type="entry name" value="MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50982">
        <property type="entry name" value="MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="12"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="repeat" description="PPR" evidence="8">
        <location>
            <begin position="94"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="8">
        <location>
            <begin position="163"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="8">
        <location>
            <begin position="198"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="8">
        <location>
            <begin position="233"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="8">
        <location>
            <begin position="268"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="8">
        <location>
            <begin position="303"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="8">
        <location>
            <begin position="338"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="8">
        <location>
            <begin position="373"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="8">
        <location>
            <begin position="408"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="8">
        <location>
            <begin position="443"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="8">
        <location>
            <begin position="478"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="8">
        <location>
            <begin position="513"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="domain" description="MBD" evidence="10">
        <location>
            <begin position="725"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="624"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="679"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="782"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="782"/>
            <end position="799"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9698414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="860" mass="95533" checksum="AF89A8E969EBC2C1" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A1Z4</accession>
    <name>A0AA39A1Z4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">60S acidic ribosomal protein P2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_008171</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Plays an important role in the elongation step of protein synthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">P1 and P2 exist as dimers at the large ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic ribosomal protein P1/P2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9699453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A1Z4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002182">
        <property type="term" value="P:cytoplasmic translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05833">
        <property type="entry name" value="Ribosomal_P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1410:FF:000002">
        <property type="entry name" value="60S acidic ribosomal protein P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01478">
        <property type="entry name" value="Ribosomal_L12_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038716">
        <property type="entry name" value="P1/P2_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027534">
        <property type="entry name" value="Ribosomal_P1/P2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044076">
        <property type="entry name" value="Ribosomal_P2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21141">
        <property type="entry name" value="60S ACIDIC RIBOSOMAL PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21141:SF115">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN P2W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00428">
        <property type="entry name" value="Ribosomal_60s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="111"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="122"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9699453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="155" mass="16231" checksum="5BB5502B5018F129" modified="2024-01-24" version="1">MDEPPQLHFTAAPLLCKTLATQEPEGRRSHRKMKVIAAYLLAVLGGNTCPSAEDLKDILGSVGAEADDDRIELLLAEVKGKDITELIASGREKLASVPSGGGAAVAVAAPGGGGGAAPAAAEPKKEEKVEEKEESDDVRLLLLLFLNSNFLFLVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A228</accession>
    <name>A0AA39A228_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ferredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_008214</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
        <text evidence="11">Binds 1 [2Fe-2S] cluster.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Plastid</location>
            <location evidence="2 11">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the 2Fe2S plant-type ferredoxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9699513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A228"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022900">
        <property type="term" value="P:electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000014">
        <property type="entry name" value="Ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006058">
        <property type="entry name" value="2Fe2S_fd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010241">
        <property type="entry name" value="Fd_pln"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02008">
        <property type="entry name" value="fdx_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43112">
        <property type="entry name" value="FERREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43112:SF30">
        <property type="entry name" value="FERREDOXIN-3, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00111">
        <property type="entry name" value="Fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00197">
        <property type="entry name" value="2FE2S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="5 11" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="8 11" id="KW-0249">Electron transport</keyword>
    <keyword evidence="9 11" id="KW-0408">Iron</keyword>
    <keyword evidence="10 11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0934">Plastid</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0813">Transport</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="12">
        <location>
            <begin position="57"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU364001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9699513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="151" mass="16275" checksum="9BA25D431D006B32" modified="2024-01-24" version="1">MSTINLPTHCMFRSATQNRIASAFIRSPSSLGSVKSISKAFGLKSSPCFRATAMAVYKIKLIGPEGEEHEFDAPDDAYILDSAENAGVELPYSCRAGACSTCAGQLVSGSVDQSDGSFLDEKQMEKGYLLTCISYPTSDCVIHTHKESDLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A4T4</accession>
    <name>A0AA39A4T4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Galectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_005646</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyltransferase 31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9699884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A4T4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008378">
        <property type="term" value="F:galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901137">
        <property type="term" value="P:carbohydrate derivative biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00070">
        <property type="entry name" value="GLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.50:FF:000015">
        <property type="entry name" value="Beta-1,3-galactosyltransferase GALT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001079">
        <property type="entry name" value="Galectin_CRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002659">
        <property type="entry name" value="Glyco_trans_31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214:SF3">
        <property type="entry name" value="BETA-1,3-GALACTOSYLTRANSFERASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214">
        <property type="entry name" value="BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00337">
        <property type="entry name" value="Gal-bind_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01762">
        <property type="entry name" value="Galactosyl_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00908">
        <property type="entry name" value="Gal-bind_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51304">
        <property type="entry name" value="GALECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="13" id="KW-0464">Manganese</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Galectin" evidence="14">
        <location>
            <begin position="169"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9699884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="637" mass="71971" checksum="5800E1549CB7AC06" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A5L6</accession>
    <name>A0AA39A5L6_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_006499</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A5L6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030132">
        <property type="term" value="C:clathrin coat of coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030130">
        <property type="term" value="C:clathrin coat of trans-Golgi network vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016025">
        <property type="entry name" value="Clathrin_H-chain_N"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50989">
        <property type="entry name" value="Clathrin heavy-chain terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9701179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="211" mass="23960" checksum="5F01D6771B34C038" modified="2024-01-24" version="1">MKEAPTLPSLGVSRLRMSRWNLPLRRTITADSTLMNPNTRILALKAQLPGTTADLNPFLDQFGSGSTRLETSVAAFDVYFLIFRGDVFINRLYYVDVGGMEGSTTCAPNNQACCHTERRSWDHARACIAKEAPFSCKLRSEKWRTVATGSARSPNASLRVDDRRDWLWIRNPNQCLSRTRYAPQNSSSVRIFCLKLENFQTRKDATYKEIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A5V8</accession>
    <name>A0AA39A5V8_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_006791</name>
        <name evidence="2" type="ORF">PVL29_006797</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A5V8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9701568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9701574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="101" mass="11881" checksum="8316DF4483661A08" modified="2024-01-24" version="1">MEKLNVVLIQEPVVLVGVEEEVQWIQRKLMRLGVIHGYDFIEELMDVAYNFEDERCILLVRIHKKQEMIKSKISHPPARIAVPYSLEIPEYLEEMELFPSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2025-10-08" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A789</accession>
    <name>A0AA39A789_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_006504</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35746">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT (PPR) SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35746:SF1">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT (PPR) SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="C2H2-type" evidence="4">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="1302"/>
            <end position="1336"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="1337"/>
            <end position="1371"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="1372"/>
            <end position="1406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="59"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="253"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="725"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="776"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="899"/>
            <end position="987"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1047"/>
            <end position="1137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1164"/>
            <end position="1234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="82"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="725"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="917"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="956"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1107"/>
            <end position="1119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1193"/>
            <end position="1202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9701188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1635" mass="177328" checksum="A956D2DA733F81E1" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A8V5</accession>
    <name>A0AA39A8V5_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_004776</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A8V5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37891">
        <property type="entry name" value="OS06G0113900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37891:SF1">
        <property type="entry name" value="OS06G0113900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="63"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="168"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="193"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="238"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="272"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="327"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="355"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="389"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="421"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="453"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="487"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="519"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9703143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="540" mass="59062" checksum="376D2FF87AA0A413" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A914</accession>
    <name>A0AA39A914_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein pelota homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_004857</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the Pelota-HBS1L complex, a complex that recognizes stalled ribosomes and triggers the No-Go Decay (NGD) pathway. In the Pelota-HBS1L complex, pelo recognizes ribosomes stalled at the 3' end of an mRNA and engages stalled ribosomes by destabilizing mRNA in the mRNA channel. Following ribosome-binding, the Pelota-HBS1L complex promotes the disassembly of stalled ribosomes, followed by degradation of damaged mRNAs as part of the NGD pathway.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 9">Belongs to the eukaryotic release factor 1 family. Pelota subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A914"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070651">
        <property type="term" value="P:nonfunctional rRNA decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070966">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, no-go decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070481">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, non-stop decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032790">
        <property type="term" value="P:ribosome disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071025">
        <property type="term" value="P:RNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.870:FF:000002">
        <property type="entry name" value="Protein pelota homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000008">
        <property type="entry name" value="Protein pelota homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.60:FF:000002">
        <property type="entry name" value="Protein pelota homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.60">
        <property type="entry name" value="eRF1 domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.870">
        <property type="entry name" value="Pelota, domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042226">
        <property type="entry name" value="eFR1_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005141">
        <property type="entry name" value="eRF1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005142">
        <property type="entry name" value="eRF1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005140">
        <property type="entry name" value="eRF1_Pelota-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038069">
        <property type="entry name" value="Pelota/DOM34_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058547">
        <property type="entry name" value="Pelota_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004405">
        <property type="entry name" value="TF_pelota"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00111">
        <property type="entry name" value="pelota"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10853">
        <property type="entry name" value="PELOTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10853:SF0">
        <property type="entry name" value="PROTEIN PELOTA HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03464">
        <property type="entry name" value="eRF1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03465">
        <property type="entry name" value="eRF1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26356">
        <property type="entry name" value="Pelota_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01194">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159065">
        <property type="entry name" value="Dom34/Pelota N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="eRF1/Pelota-like N-terminal" evidence="10">
        <location>
            <begin position="43"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00054141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9703236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="420" mass="47065" checksum="392A695768B96955" modified="2024-01-24" version="1">MACKKQSPSPSRSWTEPIASHSPQVLYKDSEKKRREIEVARIMKIVRRDLVANGPGSVKMVPVDSDDLWYAYNLIAPGDTVLAVTVRKVLREVASGGRDAERVKLKLEIKVEVAEYDKVGSVLRIRGKNILENEYVKIGQFHTLEIELHRPFVLRKVVWDSLALDTLNQASDPAASADLAVVLMQEGLAHVILVGRSLTITRSRIEASIPRKHGPAIAGYEAALNRFFENVLQAFLKYVDFNVVRCAVIASPGFTKDQFHRHLLLEAERRQLRSIIENKSRIILVHTSSGYKHSLKEVLDAPNVMNMIKDTKAAQEVQALKDFFNMLSNDPARACYGPKHVEVAHERMAVQTLLITDDLFRSADIPTRQKYVNFVNSVKDSGGTAHIFSSMHVSGEQLAQLTGIAAILRFPLPDLEDIEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A9M9</accession>
    <name>A0AA39A9M9_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_004204</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A9M9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000324">
        <property type="entry name" value="Transcription factor MYB3R-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000010">
        <property type="entry name" value="Transcriptional activator Myb isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000016">
        <property type="entry name" value="Transcriptional activator Myb isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF266">
        <property type="entry name" value="TRANSCRIPTION FACTOR MYB3R-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="32"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="38"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="84"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="84"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="136"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="140"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="15"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="25"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9702359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="976" mass="107074" checksum="EE6075F7A4CDF21F" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AH39</accession>
    <name>A0AA39AH39_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">VAN3-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_002076</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AH39"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010305">
        <property type="term" value="P:leaf vascular tissue pattern formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010087">
        <property type="term" value="P:phloem or xylem histogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013666">
        <property type="entry name" value="PH_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040269">
        <property type="entry name" value="VAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008546">
        <property type="entry name" value="VAN3-bd-like_auxin_canal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31351">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31351:SF24">
        <property type="entry name" value="VAN3-BINDING PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05703">
        <property type="entry name" value="Auxin_canalis"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08458">
        <property type="entry name" value="PH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VAN3-binding protein-like auxin canalisation" evidence="1">
        <location>
            <begin position="38"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="VAN3-binding protein-like auxin canalisation" evidence="1">
        <location>
            <begin position="124"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="Pleckstrin-like plant" evidence="2">
        <location>
            <begin position="290"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9706932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="411" mass="45178" checksum="D1C536BC7FFCB034" modified="2024-01-24" version="1">MEGGQFGGQKMGNLHDLEHAEDGDELKLWSQLPSIPQPQTPKEPMEFLSRSWSLSALEISKALKEKQCVLDKNPSIIPEMVAAPQLVSYPLVTAALLSLLSVPPYLIYVYMDHIFLQTGKVMNWVNTRRTVSIGKWFHHKELGSSGVKKKDRMRVENARVHATMSVAGLAAALASAAAAENSSGSGSKMSAALASATELLASHCIEIAESAGADHDRLASVVRSAVDIQSPGDLTTLTAAAATALRGEAALKVRLQKETRKNASISPYEAPFVADFHSEMEEQDPPCTGELQQHTQKGVLRWKRVTIYINKKSQVIVKLKSKHVGGAFSKKIKCVVYGVCNETAAWPFRKERENIEAYFGLKTAQGFLEFKCKSKIHKQTWVDGIQNLLCRVSCIEATEHSLEILNINKNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AI62</accession>
    <name>A0AA39AI62_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_000240</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AI62"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35831">
        <property type="entry name" value="OS01G0642200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35831:SF2">
        <property type="entry name" value="OS01G0642200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="54"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9708081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="64" mass="6695" checksum="59EFC4449E7F3D61" modified="2024-01-24" version="1">MASLKAQKPLTTQPAGQVKKEPAKPSGNPPNPPTPVSKPGPKKAEHKPREPKKAPASKPAQAKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AJM1</accession>
    <name>A0AA39AJM1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_000642</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RRM CPSF6/7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AJM1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12372">
        <property type="entry name" value="RRM_CFIm68_CFIm59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034772">
        <property type="entry name" value="CPSF6/7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23204">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFIC FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="238"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="326"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="513"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="327"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="346"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="520"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="546"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="632"/>
            <end position="656"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9708721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="656" mass="70285" checksum="401FD3B1BF0A8AEB" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AJR3</accession>
    <name>A0AA39AJR3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">protein-disulfide reductase</fullName>
            <ecNumber evidence="1">1.8.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_000757</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>[protein]-dithiol + NAD(+) = [protein]-disulfide + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18749"/>
            <dbReference type="Rhea" id="RHEA-COMP:10593"/>
            <dbReference type="Rhea" id="RHEA-COMP:10594"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.8.1.8"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>[protein]-dithiol + NADP(+) = [protein]-disulfide + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18753"/>
            <dbReference type="Rhea" id="RHEA-COMP:10593"/>
            <dbReference type="Rhea" id="RHEA-COMP:10594"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.8.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the nucleoredoxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.8" evidence="1"/>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AJR3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004791">
        <property type="term" value="F:thioredoxin-disulfide reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03009">
        <property type="entry name" value="TryX_like_TryX_NRX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004146">
        <property type="entry name" value="DC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052259">
        <property type="entry name" value="Nucleoredoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012336">
        <property type="entry name" value="Thioredoxin-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045870">
        <property type="entry name" value="TryX_NRX_thioredoxin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13871">
        <property type="entry name" value="THIOREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13871:SF96">
        <property type="entry name" value="THIOREDOXIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03107">
        <property type="entry name" value="C1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13905">
        <property type="entry name" value="Thioredoxin_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Thioredoxin" evidence="8">
        <location>
            <begin position="13"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="8">
        <location>
            <begin position="322"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9708918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="573" mass="65255" checksum="C9EA01A487558294" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AKA8</accession>
    <name>A0AA39AKA8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosomal protein S21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_000670</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bS21 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AKA8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001911">
        <property type="entry name" value="Ribosomal_bS21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37228">
        <property type="entry name" value="RIBOSOMAL PROTEIN S21 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37228:SF1">
        <property type="entry name" value="RIBOSOMAL PROTEIN S21 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01165">
        <property type="entry name" value="Ribosomal_S21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9708769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="125" mass="14387" checksum="BDDF95D3CF112531" modified="2024-01-24" version="1">MSHIARHVSNICGRPSLAPNSLGCELQQLRGIRVKVLNGNLESALIVMQRKMQSSGIERLIKREQTHHIKNSEKRVLARKNLERRIQSQELARKLKAILIKKVSNTLFKWGLNDVFFTSISFRQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ALQ1</accession>
    <name>A0AA39ALQ1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">NAD(P)H-quinone oxidoreductase subunit L, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_000482</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a plastoquinone + NADH + (n+1) H(+)(in) = a plastoquinol + NAD(+) + n H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:42608"/>
            <dbReference type="Rhea" id="RHEA-COMP:9561"/>
            <dbReference type="Rhea" id="RHEA-COMP:9562"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17757"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:62192"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a plastoquinone + NADPH + (n+1) H(+)(in) = a plastoquinol + NADP(+) + n H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:42612"/>
            <dbReference type="Rhea" id="RHEA-COMP:9561"/>
            <dbReference type="Rhea" id="RHEA-COMP:9562"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17757"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:62192"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ALQ1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016655">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019654">
        <property type="entry name" value="NADH-quinone_OxRdatse_su_L"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36727">
        <property type="entry name" value="NAD(P)H-QUINONE OXIDOREDUCTASE SUBUNIT L, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36727:SF2">
        <property type="entry name" value="NAD(P)H-QUINONE OXIDOREDUCTASE SUBUNIT L, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10716">
        <property type="entry name" value="NdhL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0618">Plastoquinone</keyword>
    <keyword evidence="3" id="KW-0874">Quinone</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0793">Thylakoid</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <keyword evidence="2 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="89"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="127"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9708464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="188" mass="21997" checksum="6D7F1D1217B69787" modified="2024-01-24" version="1">MSCSFSIQIPKALPCLFHSQCKFPSLTISSIQKQSHHAKPIKTITSIINKSVDYISTNKHSLAIQVGALLATVEKPAFAVTGVNKEVDLIWVLIQLGIVLFWYFLIMPPIIMNWLRIRWYERNLLEMYLQFMFVFIFFPGVMLWAPFLNFRKFPRDPSMKYPWSTPQNPTQVKNAFLKYPFAKPEDYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AMR7</accession>
    <name>A0AA39AMR7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_001463</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AMR7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000583">
        <property type="entry name" value="Inactive leucine-rich repeat receptor-like protein kinase CORYNE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000669">
        <property type="entry name" value="Inactive leucine-rich repeat receptor-like protein kinase CORYNE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051824">
        <property type="entry name" value="LRR_Rcpt-Like_S/T_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48006">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN DDB_G0281931-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48006:SF100">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE_THREONINE-KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041233621" description="Protein kinase domain-containing protein" evidence="3">
        <location>
            <begin position="26"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="54"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="109"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9709999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="390" mass="43606" checksum="5002AA013A7573F8" modified="2024-01-24" version="1">MEKTSREAFSLLFLLIFIHFYNVKCQENIINRVSTDSSPSPSREAEFKSGLKKIILSIVFGVLTGLLSAALCAGIVHCVVRYMNRTPILKGPVVFSPKIAPKTVQSALDSENQLLGSSPNGKYYKTALDNGLIIAVKRLEPFETGSPETQSKSVKRRIQQELEMLANLRHRNLMSLRAYVRDSDRFSLVYDYVPTGSLEDAMNRVRENQLQLSWEVRLRIAVGVIKGLQYLHFNCVPRILHYNLKPTNVMLDAEFEPRLADCGLAKLMPNLDRAASGYSAPECFQNCRYTDKSDVFSFGVILGVLLTGRDPMDPFFSEAAGGGSLGRWLRHLQQAGEAREALDKSILGEEVEEDEMLMAVRIAVVCLSDLPADRPSSDELVPMLTQLHSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D353</accession>
    <name>A0AA39D353_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein SKIP34</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_026663</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D353"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="30"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9670253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="97" mass="11627" checksum="FC084CD20B3B573E" modified="2024-01-24" version="1">MCYDQLRSLSEDEIIPPRTTRSPDENAVVVEDLRSRLAETQARLERARAREAELSRRLQEMKRFVSVMEILETYLKRRYQEQQQHVERLLSVIPQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D6I8</accession>
    <name>A0AA39D6I8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HMA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_023366</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D6I8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22814">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN ATOX1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22814:SF336">
        <property type="entry name" value="HEAVY METAL-ASSOCIATED ISOPRENYLATED PLANT PROTEIN 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMA" evidence="2">
        <location>
            <begin position="25"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9673763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="149" mass="16446" checksum="3524DFC99FC852C6" modified="2024-01-24" version="1">MGVGATLEYFSDLLISKKGKKKKHLQTVDLKVRMDCEGCELKVKKALSSLKGVKSVDINLKQQKASVTGYADAKKVLKKARSTGKKAELWPYVPYNLVAHPYVAQVYDKKAPPGYVRGSENPAITGMSPVEEQYTTMFSDENPNACSIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D6M0</accession>
    <name>A0AA39D6M0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">S-protein homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_025992</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the plant self-incompatibility (S1) protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D6M0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060320">
        <property type="term" value="P:rejection of self pollen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010264">
        <property type="entry name" value="Self-incomp_S1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31232">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31232:SF149">
        <property type="entry name" value="S-PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05938">
        <property type="entry name" value="Self-incomp_S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 6" id="KW-0713">Self-incompatibility</keyword>
    <keyword evidence="5 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041482167" description="S-protein homolog" evidence="6">
        <location>
            <begin position="25"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9672594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="172" mass="20103" checksum="0D432BEBEBE76729" modified="2024-01-24" version="1">MFLPFSRYLFSFVLLLFLVRLCDGGPWVVEKKVDLRITNDLGSGLDLNLHCQSKEDDLGTHVLAPDQFFEFRFRPNFWGTTLYFCRFWWGGESHWFNIYVEKRDTSRCDSQCWWMVGPAGPCLLDDRIGLYDLCENWNDKQVEGSSKKGTGKDAARVYNDTKYLEGNEKRGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D7G6</accession>
    <name>A0AA39D7G6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_026245</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D7G6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000368">
        <property type="entry name" value="cold-responsive protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000225">
        <property type="entry name" value="cold-responsive protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052059">
        <property type="entry name" value="CR_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47973">
        <property type="entry name" value="CYSTEINE-RICH RECEPTOR-LIKE PROTEIN KINASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="46"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9672910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="385" mass="42774" checksum="76FA78EA2D66DACC" modified="2024-01-24" version="1">MTWFSCFGRRATSPAGGTDEVDEEVSGIHNVRLYTYKELKNATDDFSPANKIGEGGFGSVYKAKLKDGTIAAIKVLSAESKQGVREFLTEINVISNIEHEYLVKLYGCCVEANHRILVYNFLENNSLAQTLLGGGSSSMQFSWRTRSRICIGVARGLAFLHEEVRPYIVHRDIKASNILLDRNLNPKIADFGLAKLIPSNMTHVSTRVAGTIGYLAPEYAIRGQLTRKADIYSFGVLLVEIVCGRCNTNTRLPIGEQYLLERTWELYERKELVGLVDESLNGDFDAEEACRFLKIGLLCTQDTPKLRPSMSSVVKMLIGEMDVDSRAITKPGLISDFMDLKIRSEPKPVPDAKNVSDNLSSGWDNQDNSTMSSGITFTAVYDRSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D7R6</accession>
    <name>A0AA39D7R6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Kinesin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_024118</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D7R6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000141">
        <property type="entry name" value="Kinesin-like protein KIN-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24115:SF1008">
        <property type="entry name" value="KINESIN-LIKE PROTEIN SUBITO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24115">
        <property type="entry name" value="KINESIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0493">Microtubule</keyword>
    <keyword evidence="4 5" id="KW-0505">Motor protein</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="8">
        <location>
            <begin position="69"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="85"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="813"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="851"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="921"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="547"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="851"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="922"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="191"/>
            <end position="198"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9675023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1189" mass="131238" checksum="F541A730E6097495" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DA99</accession>
    <name>A0AA39DA99_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Potassium channel</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_025130</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Potassium channel.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">The potassium channel is composed of a homo- or heterotetrameric complex of pore-forming subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="14">The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids. The pore-forming region H5 is enclosed by the transmembrane segments S5 and S6 in the Shaker-type (1P/6TM) and contains the GYGD signature motif which seems to be involved in potassium selectivity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the potassium channel family. Plant (TC 1.A.1.4) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9676449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DA99"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000074">
        <property type="entry name" value="Potassium channel KAT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000123">
        <property type="entry name" value="Potassium channel KAT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.630">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003938">
        <property type="entry name" value="K_chnl_volt-dep_EAG/ELK/ERG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045319">
        <property type="entry name" value="KAT/AKT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45743">
        <property type="entry name" value="POTASSIUM CHANNEL AKT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45743:SF4">
        <property type="entry name" value="POTASSIUM CHANNEL KOR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01463">
        <property type="entry name" value="EAGCHANLFMLY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="12 14" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10 14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 14" id="KW-0630">Potassium</keyword>
    <keyword evidence="6 14" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4 14" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 14" id="KW-0813">Transport</keyword>
    <keyword evidence="7 14" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="63"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="97"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="245"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="277"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="15">
        <location>
            <begin position="377"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="13">
        <location>
            <begin position="554"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="13">
        <location>
            <begin position="587"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="13">
        <location>
            <begin position="651"/>
            <end position="683"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU369015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9676449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="720" mass="82247" checksum="88AD3C6BE39630D6" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DAV9</accession>
    <name>A0AA39DAV9_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_022998</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9678263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DAV9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9678263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="142" mass="15696" checksum="2CB7C01165E6AE0F" modified="2024-01-24" version="1">MLLCMWLLNKINVAYFHIDLDGGVAIAMTASDGQFLLDHLINGVNDFKQHGGSYVRGCLLFSQVFYVSCSHFPSMTVPSSLPRCAHWIDVLVRKRVKLDVKMLGALSHVQFHCALLNEAVENNGSMNDNNVATVIGVGNEFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DDQ0</accession>
    <name>A0AA39DDQ0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_021740</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DDQ0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12384">
        <property type="entry name" value="RRM_RBM24_RBM38_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11176">
        <property type="entry name" value="BOULE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11176:SF22">
        <property type="entry name" value="RNA-BINDING PROTEIN 38-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="50"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="127"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="10"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9679930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="295" mass="30579" checksum="A6547924D0D75F50" modified="2024-01-24" version="1">MSHPRQYQMVGGGGGGGGGGGSGGGGGGGGGGGGGGGGGNATQFGDTTYTKIFVGGLAWETHRETLKRYFEQFGEILEAVVITDKVSGKSKGYGFVTFKDPDSAMRACQDPSPVIDGRRANCNIASLGAHKTRPPPHHGMERLRPASGSMGKPSHHGSSTNFRQSNPHFSFPPYSVYGYSGYPQELYAMNYYNVYSGQQMSSYYSSPSGSPGVYYGYYPVYTQQGQSSQMQGFGIQYPQTTQYPYLPQPYPAVGILPLPASASASASASLGATATGAATAAGEVTRTAAASEQGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DF34</accession>
    <name>A0AA39DF34_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RIC1 C-terminal alpha solenoid region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_020302</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9681355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DF34"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034066">
        <property type="term" value="C:Ric1-Rgp1 guanyl-nucleotide exchange factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001743">
        <property type="entry name" value="Protein RIC1 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040096">
        <property type="entry name" value="Ric1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009771">
        <property type="entry name" value="RIC1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22746:SF10">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR SUBUNIT RIC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22746">
        <property type="entry name" value="RAB6A-GEF COMPLEX PARTNER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25440">
        <property type="entry name" value="Beta-prop_RIC1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07064">
        <property type="entry name" value="RIC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RIC1 C-terminal alpha solenoid region" evidence="3">
        <location>
            <begin position="763"/>
            <end position="938"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9681355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1126" mass="127468" checksum="008EFCCB3E231792" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DFI9</accession>
    <name>A0AA39DFI9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">L18 ribosomal protein Heart Stopper</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_018178</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DFI9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008097">
        <property type="term" value="F:5S rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00432">
        <property type="entry name" value="Ribosomal_L18_L5e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.100:FF:000004">
        <property type="entry name" value="50S ribosomal protein L18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057268">
        <property type="entry name" value="Ribosomal_L18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005484">
        <property type="entry name" value="Ribosomal_uL18_bac/plant/anim"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12899">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L18, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12899:SF5">
        <property type="entry name" value="RIBOSOMAL L18P_L5E FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00861">
        <property type="entry name" value="Ribosomal_L18p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9682166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="187" mass="21168" checksum="A47918F1BFDBD2C2" modified="2024-01-24" version="1">MLKHLSGKLWNKVVWANGRDQFRPCLHVPTSGFHNGKAHLAPRSFFGVEDFLDDDNSRPYTYQKEKKSKNPNKHISFKQRTGAYMEPFTLDVLISKRFVSASLTHRVTSKQVAVAGTNSKDIKAVLKSRSDIPACLAIGRILSERAREADVYTASYTPRDRDKFEGKIRAVVQSLIDNGIDIKVYLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DGD2</accession>
    <name>A0AA39DGD2_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_018836</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GLUTAMINE DUMPER 1 (TC 9.B.60) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DGD2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080143">
        <property type="term" value="P:regulation of amino acid export"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040359">
        <property type="entry name" value="GDU"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33228">
        <property type="entry name" value="PROTEIN GLUTAMINE DUMPER 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33228:SF76">
        <property type="entry name" value="PROTEIN GLUTAMINE DUMPER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="50"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9683006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="101" mass="10994" checksum="F92298A3D1020A5E" modified="2024-01-24" version="1">MRPANSSSSSNEIWRWNSPFPYLFGGLALILGLIVVALIILSCSHKKRTPSPDHDKDKPPKTICTEPDTVPRVVVIMAGDENTTYLATPVPTSTTASDEQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DGP9</accession>
    <name>A0AA39DGP9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="15">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PVL29_019138</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 15">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Membrane</location>
            <topology evidence="1 15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="15"/>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DGP9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140326">
        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02073">
        <property type="entry name" value="P-type_ATPase_APLT_Dnf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000042">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000014">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF165">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 14 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 13 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 15" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="82"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="106"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="284"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="336"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="907"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="939"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="986"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1024"/>
            <end position="1047"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1054"/>
            <end position="1073"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1093"/>
            <end position="1113"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="16">
        <location>
            <begin position="40"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="17">
        <location>
            <begin position="872"/>
            <end position="1122"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="12">
        <location>
            <position position="408"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="408"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="408"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="409"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="410"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="410"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="526"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="573"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="596"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="630"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="711"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="712"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
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        <location>
            <position position="713"/>
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        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="820"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="826"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="846"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="849"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="850"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
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    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="850"/>
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        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU362033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF16209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF16212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ9683426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1163" mass="132284" checksum="2F1E3A2C4F5826D2" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DH65</accession>
    <name>A0AA39DH65_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">NF-X1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_016366</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NFX1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9683829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DH65"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06008">
        <property type="entry name" value="NF-X1-zinc-finger"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="CDD" id="cd16697">
        <property type="entry name" value="RING-CH-C4HC3_NFXL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034078">
        <property type="entry name" value="NFX1_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000967">
        <property type="entry name" value="Znf_NFX1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12360:SF1">
        <property type="entry name" value="NF-X1-TYPE ZINC FINGER PROTEIN NFXL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12360">
        <property type="entry name" value="NUCLEAR TRANSCRIPTION FACTOR, X-BOX BINDING 1 NFX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01422">
        <property type="entry name" value="zf-NF-X1"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00438">
        <property type="entry name" value="ZnF_NFX"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="831"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="domain" description="NF-X1-type" evidence="8">
        <location>
            <begin position="189"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="NF-X1-type" evidence="8">
        <location>
            <begin position="241"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="NF-X1-type" evidence="8">
        <location>
            <begin position="294"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="NF-X1-type" evidence="8">
        <location>
            <begin position="348"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="NF-X1-type" evidence="8">
        <location>
            <begin position="401"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="domain" description="NF-X1-type" evidence="8">
        <location>
            <begin position="428"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="domain" description="NF-X1-type" evidence="8">
        <location>
            <begin position="491"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="domain" description="NF-X1-type" evidence="8">
        <location>
            <begin position="596"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="domain" description="NF-X1-type" evidence="8">
        <location>
            <begin position="639"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="domain" description="NF-X1-type" evidence="8">
        <location>
            <begin position="702"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="529"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9683829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="874" mass="97623" checksum="E3356410D961C878" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DHP8</accession>
    <name>A0AA39DHP8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_016701</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DHP8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000161">
        <property type="entry name" value="Wall-associated receptor kinase-like 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000446">
        <property type="entry name" value="Wall-associated receptor kinase-like 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46008">
        <property type="entry name" value="LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-LIKE 1.4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46008:SF25">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041319472" description="Protein kinase domain-containing protein" evidence="14">
        <location>
            <begin position="22"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="300"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="254"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="328"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9684354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="589" mass="64506" checksum="B22A92A7B40F2F5E" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DK70</accession>
    <name>A0AA39DK70_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_016005</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DK70"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9687341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="131" mass="16052" checksum="02551C6A38B13F8D" modified="2024-01-24" version="1">MKKGRTCLRRNRIHFFNFNTHDKDLSSFPVKRPTWAYIQDTEWDCRIHFVSHIQRLKYMESNSILFHEESTLTMYKLVIELMFDNENSEKVIILNKIHIHAKTNKQTNKRRKYRIQSRVDFFIYMMSKCSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DL99</accession>
    <name>A0AA39DL99_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_016260</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DL99"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057135">
        <property type="entry name" value="At4g27190-like_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050905">
        <property type="entry name" value="Plant_NBS-LRR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463:SF179">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23247">
        <property type="entry name" value="LRR_RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NB-ARC" evidence="8">
        <location>
            <begin position="99"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="10">
        <location>
            <begin position="476"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein At4g27190-like leucine-rich repeats" evidence="9">
        <location>
            <begin position="676"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="903"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="903"/>
            <end position="913"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9687700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="934" mass="107153" checksum="63B00EE9370A82C0" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DMU6</accession>
    <name>A0AA39DMU6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ENTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_014804</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Clathrin-coated vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <location evidence="3">Clathrin-coated pit</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9689305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DMU6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005545">
        <property type="term" value="F:1-phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032050">
        <property type="term" value="F:clathrin heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048268">
        <property type="term" value="P:clathrin coat assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006900">
        <property type="term" value="P:vesicle budding from membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03564">
        <property type="entry name" value="ANTH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000005">
        <property type="entry name" value="Clathrin assembly protein AP180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.150:FF:000003">
        <property type="entry name" value="Putative clathrin assembly protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.150">
        <property type="entry name" value="ANTH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011417">
        <property type="entry name" value="ANTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014712">
        <property type="entry name" value="ANTH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048050">
        <property type="entry name" value="ANTH_N_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045192">
        <property type="entry name" value="AP180-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951">
        <property type="entry name" value="CLATHRIN ASSEMBLY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951:SF5">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-BINDING CLATHRIN ASSEMBLY PROTEIN LAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07651">
        <property type="entry name" value="ANTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89009">
        <property type="entry name" value="GAT-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0168">Coated pit</keyword>
    <keyword evidence="8" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="4" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="10">
        <location>
            <begin position="24"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="311"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="319"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9689305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="586" mass="65759" checksum="E2BE204B5A199C1C" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DQ72</accession>
    <name>A0AA39DQ72_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">S-protein homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_013784</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the plant self-incompatibility (S1) protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DQ72"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060320">
        <property type="term" value="P:rejection of self pollen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010264">
        <property type="entry name" value="Self-incomp_S1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05938">
        <property type="entry name" value="Self-incomp_S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0713">Self-incompatibility</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9691705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="130" mass="15237" checksum="B1E6FFFBF3134F38" modified="2024-01-24" version="1">MDLTLLRKFQAFILIILNICLCASAVAYVHVHMTNDLGHDTIIYLHCLRDAKEMGHQQIPYKWTCQWKFEQTLSSTLLCDANLQGAKELRFLAFNESGESCRSSCGWNFTQHGVFQYDKGEWKFRYNWPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DQL0</accession>
    <name>A0AA39DQL0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_012250</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the PP2C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9693398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DQL0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000015">
        <property type="entry name" value="probable protein phosphatase 2C 60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832:SF840">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C 60-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0464">Manganese</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="11">
        <location>
            <begin position="23"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="335"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="348"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9693398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="361" mass="39538" checksum="D99F7B72A9AB00B5" modified="2024-01-24" version="1">MGIYLSSPRKEKSSEDGENARVRYGLSSMQGWRTTMEDAHAAYPDLDSSTSFFAVYDGHGGAGVAKFCAKYLHEQVLKNEACSAGDLGGSVRKAFLRMDEMMRGERGWRELAMFEDKVDRFSRTNFDSMCSPMSDEFNDQKDDWTEEGPNYDFRGPNSGSTACVAIIRGDQVLVANAGDSRCVLSRAGEAYDLSTDHKPELQEEKERILKAGGSIQHGRVNGVLNLARAIGDNEFKMNKALPAEKQMVTANPEINTASLCNDDDFMVLACDGIWDCMTSQELVEFVHEQLNSGCKLSAVCEKVLDKCLAPSSGGEGCDNMTMILVQFKKPIHSSASAGKQEPLPSDIPSGSNQTPTVTNSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DQN1</accession>
    <name>A0AA39DQN1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">NB-ARC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_011595</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DQN1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.430:FF:000003">
        <property type="entry name" value="Probable disease resistance protein At5g66910"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050905">
        <property type="entry name" value="Plant_NBS-LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463:SF220">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NB-ARC" evidence="7">
        <location>
            <begin position="137"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="8">
        <location>
            <begin position="383"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="9">
        <location>
            <begin position="511"/>
            <end position="686"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9692604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="839" mass="95133" checksum="E4ECEA354A840682" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DRI9</accession>
    <name>A0AA39DRI9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Nuclear factor related to kappa-B-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_010197</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DRI9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20401">
        <property type="entry name" value="Tudor_AtPTM-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024867">
        <property type="entry name" value="NFRKB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057748">
        <property type="entry name" value="NFRKB_WH_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047365">
        <property type="entry name" value="Tudor_AtPTM-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13052:SF0">
        <property type="entry name" value="DNA-BINDING PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13052">
        <property type="entry name" value="NFRKB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21743">
        <property type="entry name" value="PTM_DIR17_Tudor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25793">
        <property type="entry name" value="WHD_2nd_NFRKB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PTM/DIR17-like Tudor" evidence="2">
        <location>
            <begin position="72"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Nuclear factor related to kappa-B-binding protein second winged helix" evidence="3">
        <location>
            <begin position="701"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="246"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="732"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="838"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF21743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9694601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="866" mass="97135" checksum="E56F0CC911714F97" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DU49</accession>
    <name>A0AA39DU49_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PORR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_009960</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DU49"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021099">
        <property type="entry name" value="PORR_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045040">
        <property type="entry name" value="PORR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31476">
        <property type="entry name" value="PROTEIN WHAT'S THIS FACTOR 1 HOMOLOG, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31476:SF12">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11955">
        <property type="entry name" value="PORR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PORR" evidence="1">
        <location>
            <begin position="43"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF11955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9694237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="441" mass="51928" checksum="66FCD4AB8A42C268" modified="2024-01-24" version="1">MRALQMKAPTSQPFPFGPFNSAIQRRWQKPADTAQTRLENRTRDSQLDKLTANLKRLATVLQLQHLISKRRGPHVSVQIMSRWKNIVGLNVGMGELLRKYPHIFEVFTHPVKRNQCCRVSGRMVDLMLQEEAAVKECELEAVRRLKKLLMMSVSGTLRVHALWLIRRELGLPENFRDSIIAKYYDDFKLVDLETVELVRRDEDLGVAQVEIWREREYREKWLSEFETKYAFPINLPTGFKIESGFREKLKNWQRLQYLKPYERKEVVRVRTCGGIERFEKWAVGILHELLSLTIEKMVEVERLSHFRRDFCMEVNVRELLLKHPGIFYISTRGNTQMVFLREAYRRGCLIEPNPVYVVRRKMLDLLLLGWRHSRELHIESKEESDNVVGDEDVGGPRDGDWVIPILDNFDDNLCEISDSSVEELSDYSENENCRNEHKLEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DVK1</accession>
    <name>A0AA39DVK1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Copper transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_007567</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the copper transporter (Ctr) (TC 1.A.56) family. SLC31A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DVK1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005375">
        <property type="term" value="F:copper ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007274">
        <property type="entry name" value="Cop_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483:SF24">
        <property type="entry name" value="COPPER TRANSPORTER 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 31 COPPER TRANSPORTERS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04145">
        <property type="entry name" value="Ctr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0186">Copper</keyword>
    <keyword evidence="3 6" id="KW-0187">Copper transport</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="34"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="74"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9698558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="98" mass="10880" checksum="AC8227A9FDF4B66F" modified="2024-01-24" version="1">MKATAMPPMHHHMTLFWGKDTGILFSGWPRARSGMYAVGLVFVFVMAFIVEWLSHSRLIKPGSSTHVTPGLVHALRMGLAYMVMLALMSFNGGVFRGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DX32</accession>
    <name>A0AA39DX32_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein EARLY FLOWERING 4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_007643</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EARLY FLOWERING 4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DX32"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009649">
        <property type="term" value="P:entrainment of circadian clock"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042753">
        <property type="term" value="P:positive regulation of circadian rhythm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048511">
        <property type="term" value="P:rhythmic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040462">
        <property type="entry name" value="EARLY_FLOWERING_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009741">
        <property type="entry name" value="EARLY_FLOWERING_4_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33469:SF1">
        <property type="entry name" value="PROTEIN ELF4-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33469">
        <property type="entry name" value="PROTEIN ELF4-LIKE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07011">
        <property type="entry name" value="Elf4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein EARLY FLOWERING 4" evidence="6">
        <location>
            <begin position="26"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="103"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="16"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="104"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9698680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="125" mass="14364" checksum="477DC237B0984DEF" modified="2024-01-24" version="1">MGDGPGDNTHCRSLEDKDEEEEEEGCDAEAWETLSRSFDQVQSVLDQNRLLIQQVNENQQSRIHDNLVKNVSLIREINGNFCKVISIYSKLSADFVDIVRRRRAENDGDEKKKEESVDLNLPLSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DXF3</accession>
    <name>A0AA39DXF3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Argonaute 10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_005989</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the argonaute family. Ago subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DXF3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02846">
        <property type="entry name" value="PAZ_argonaute_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04657">
        <property type="entry name" value="Piwi_ago-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000110">
        <property type="entry name" value="Argonaute 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000013">
        <property type="entry name" value="protein argonaute 10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.260.10:FF:000001">
        <property type="entry name" value="Protein argonaute-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.260.10">
        <property type="entry name" value="paz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014811">
        <property type="entry name" value="ArgoL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032472">
        <property type="entry name" value="ArgoL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032473">
        <property type="entry name" value="Argonaute_Mid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032474">
        <property type="entry name" value="Argonaute_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003100">
        <property type="entry name" value="PAZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036085">
        <property type="entry name" value="PAZ_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003165">
        <property type="entry name" value="Piwi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045246">
        <property type="entry name" value="Piwi_ago-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22891">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08699">
        <property type="entry name" value="ArgoL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16488">
        <property type="entry name" value="ArgoL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16487">
        <property type="entry name" value="ArgoMid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16486">
        <property type="entry name" value="ArgoN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02170">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02171">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01163">
        <property type="entry name" value="DUF1785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00949">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00950">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101690">
        <property type="entry name" value="PAZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50821">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50822">
        <property type="entry name" value="PIWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="3" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="PAZ" evidence="7">
        <location>
            <begin position="341"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="Piwi" evidence="8">
        <location>
            <begin position="628"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="953"/>
            <end position="980"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="19"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="49"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="60"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9700463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="995" mass="111722" checksum="3DA6E687A3D92B8B" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DYT6</accession>
    <name>A0AA39DYT6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_003882</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9701858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DYT6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48017">
        <property type="entry name" value="OS05G0424000 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="70"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="191"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="284"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="325"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="385"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="411"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="450"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="8">
        <location>
            <begin position="39"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9701858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="480" mass="52609" checksum="4A43013DCE3D25F7" modified="2024-01-24" version="1">MMASEVDDNRGIQNEVCESGLGSHKVADADLDDDGRPRRTGSLWTACALVITAVIGAGVLSLAWSLAQLGWVGVSVLIIFGIITFYTSNLLAECYRCPVTGKRNYTYMQAVKANLGGKMYMACGLAQYSLLIGLAIGYTVTAAISMVAIQKSNCFHKRGHEAPCEVSHKPYMIGMGLFEIVVSQIPDIGEMWGLSVIATVTSFGYASIGAALAFSTVISGHGKRTSVTGVEVGPGITAAQKMWRMFRAIGDMLLCSSYSAILIEIQDILKSSGSEIQVMKKANMISVSTTTLFYLICACFGYAAFGNNAHGNMLTGFGFYEPFWLIDLANTFIVMHLVGAYQVVSQPVFGAVESQMRRWWPRSKFVIAEYPIRIGKKNFNLSINLLRLTWRSMFVVIITLLALALPYFNEVLALLGAISYWPLTVYFPVNMHIVQKKISRWTIRWFGLQSLNFVCLLVALAAACGSIEGFAEALHIFKHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DZC0</accession>
    <name>A0AA39DZC0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">hydroxyacylglutathione hydrolase</fullName>
            <ecNumber evidence="7">3.1.2.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Glyoxalase II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_006820</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>an S-(2-hydroxyacyl)glutathione + H2O = a 2-hydroxy carboxylate + glutathione + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21864"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58896"/>
            <dbReference type="ChEBI" id="CHEBI:71261"/>
            <dbReference type="EC" id="3.1.2.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.6" evidence="7"/>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DZC0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004416">
        <property type="term" value="F:hydroxyacylglutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019243">
        <property type="term" value="P:methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07723">
        <property type="entry name" value="hydroxyacylglutathione_hydrolase_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.15.10:FF:000019">
        <property type="entry name" value="Hydroxyacylglutathione hydrolase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01374">
        <property type="entry name" value="Glyoxalase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035680">
        <property type="entry name" value="Clx_II_MBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050110">
        <property type="entry name" value="Glyoxalase_II_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032282">
        <property type="entry name" value="HAGH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017782">
        <property type="entry name" value="Hydroxyacylglutathione_Hdrlase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03413">
        <property type="entry name" value="GSH_gloB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43705">
        <property type="entry name" value="HYDROXYACYLGLUTATHIONE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43705:SF1">
        <property type="entry name" value="HYDROXYACYLGLUTATHIONE HYDROLASE GLOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16123">
        <property type="entry name" value="HAGH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="12">
        <location>
            <begin position="86"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00006759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00011917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9701604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="329" mass="36015" checksum="336C9D8D8B689559" modified="2024-01-24" version="1">MQVISKAPSAMASFSCSSRGGSGLGVWPGMRQLCLRKSLLYGLMRLLSTPFKTLRTAGRTLKVAQFCSVSNMSSTLQIELVPCLKDNYAYLLHDVDTGTVGVVDPSEAVPVIDALSRKNRNLTYILNTHHHYDHTGGNAELKARYGAKVIGSGIDKDRIPGIDIVLKDGDKWMFAGHEVVVIETPGHTRGHISFYFPGSGAIFTGDTLFSLSCGKLFEGTPEQMHSSLSKIMSLPDETNIYCGHEYTLSNSKFALSIEPKNEVLQSYATHVAHLRSKGLPTIPTTLKMEKMCNPFLRTSSPEIRKSLKITATADDSEALGIIREAKDNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DZH5</accession>
    <name>A0AA39DZH5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_006473</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DZH5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF78">
        <property type="entry name" value="OS12G0587133 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="331"/>
            <end position="609"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9701140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1070" mass="123092" checksum="02AE575BF36C2239" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E3A6</accession>
    <name>A0AA39E3A6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Maspardin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_001982</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E3A6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026151">
        <property type="entry name" value="Maspardin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15913">
        <property type="entry name" value="ACID CLUSTER PROTEIN 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15913:SF0">
        <property type="entry name" value="MASPARDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="382"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="6">
        <location>
            <begin position="41"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="278"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="295"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9706778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="409" mass="46061" checksum="CC27D2EFB1B36375" modified="2024-01-24" version="1">MKGVFSAPGDYIHFKSQVPLHKIPIGTKQWRYYDFGPKVVPPLICLPGTAGTADVYYKQIMSLSIKGYRVISVDIPRVWNHHEWIQAFEKFLDAIDVHHIHLYGTSLGGFLAQLFAQHRPRRVRSLILSNSFLETRSFSSAMPWAPIVSWTPSFLLKRYVLTGIPDGPHEPFIADSVDFVVSQVETLSREDLASRLTLTVDAASIGPLLLSDSFITLMDTNDYCSIPQQLKDQLSERYPGARRAYLKTGGDFPFLSRSDEVNLHLQLHLRRVGVEPRPDLVKGISKDGSGGSSSEKNDEREDSDVPPKDNGGPESPETPSAETQLPEAPESSGSHSLDDQLLSNAKVCFTSPEHVRLLISHAFFEKQQDIVSTRFVQPAWEIFILCLLPLYVETMYITWIFCWKSRCLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E4W0</accession>
    <name>A0AA39E4W0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF6469 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_001198</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E4W0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045529">
        <property type="entry name" value="DUF6469"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20073">
        <property type="entry name" value="DUF6469"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6469" evidence="1">
        <location>
            <begin position="72"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9709601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="219" mass="25073" checksum="F37C50F51BB24F2B" modified="2024-01-24" version="1">MAVTPKARDLIDVVFSWSLEDICNQDFYKNKVGKIPGTFQSAEHYFASFVYPLLEETRAQLSLGLELISRAPFAELICIDKVETDEELLYNVRVDYWRNRSAGRCLDPYRTVPGDLVIFADAKLETFSDVQCLGRKTWAFALVTEVRENEIEDDGTTVCFKVRVSKETAEGDGKNKWTFMYFLINITTGERIWNVLHMSGNLNIIKQVLFTDSKVSITL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E582</accession>
    <name>A0AA39E582_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_000788</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E582"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015660">
        <property type="entry name" value="MASH1/Ascl1a-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13935">
        <property type="entry name" value="ACHAETE-SCUTE TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13935:SF118">
        <property type="entry name" value="BHLH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="6">
        <location>
            <begin position="8"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="48"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9708968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="176" mass="19762" checksum="1750A144D0E1950C" modified="2024-01-24" version="1">MPRRGRSSSRVDRNALERDRRQHTKELFSRLGFLLPIPLSKRSLPEMLDQATTHVKQLGQRVEMLKQKKQLLEGSSSTDDQTTGIRDHMMGGAWLPVLTVSDLGSMLEVCVKSGSNKKFVLHQVIQVLAEEAAQVVALSYSNVGHGIFYTINAQAVSPRIGIETSRVHERLKELIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E5P8</accession>
    <name>A0AA39E5P8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MPBQ/MBSQ family SAM-binding methyltransferase profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_001048</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class I-like SAM-binding methyltransferase superfamily. MPBQ/MBSQ MT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E5P8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051741">
        <property type="term" value="F:2-methyl-6-phytyl-1,4-benzoquinone methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022657">
        <property type="entry name" value="De-COase2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044649">
        <property type="entry name" value="MPBQ/MSBQ_MT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031164">
        <property type="entry name" value="SAM_MPBQ_MSBQ_MT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44516">
        <property type="entry name" value="2-METHYL-6-PHYTYL-1,4-HYDROQUINONE METHYLTRANSFERASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44516:SF4">
        <property type="entry name" value="2-METHYL-6-PHYTYL-1,4-HYDROQUINONE METHYLTRANSFERASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00879">
        <property type="entry name" value="ODR_DC_2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51734">
        <property type="entry name" value="SAM_MPBQ_MSBQ_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MPBQ/MBSQ family SAM-binding methyltransferase profile" evidence="5">
        <location>
            <begin position="17"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="SAM motif I" evidence="3">
        <location>
            <begin position="66"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="201"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9709388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="221" mass="25058" checksum="D73B666A677746FD" modified="2024-01-24" version="1">MTSKCSLSSSRPISQPGFIQHKQEAFWFYSFLSIVYDHIINLGHWTEDMRDEALELADFYGRNMIVVDVGGGIGFTIMDQSPRQLAKAKEKDPLKECRIIEGDIEDLSFQLIMQIDISLLEGYFHSVWKIIPTPHHITHHTSTMQPHLTTAHSWPFPSSYTSHMLTHNSPHLYHAATPHHSSLMVVSQLLCNPHAHPQLTTPPPCSHTSPQLTHGRFSASM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E7N7</accession>
    <name>A0AA39E7N7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fe-S metabolism associated domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_000676</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SufE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E7N7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003808">
        <property type="entry name" value="Fe-S_metab-assoc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43597:SF5">
        <property type="entry name" value="SUFE-LIKE PROTEIN 2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43597">
        <property type="entry name" value="SULFUR ACCEPTOR PROTEIN CSDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02657">
        <property type="entry name" value="SufE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82649">
        <property type="entry name" value="SufE/NifU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe-S metabolism associated" evidence="2">
        <location>
            <begin position="68"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9708775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="212" mass="23513" checksum="41687DFC6CE68FC3" modified="2024-01-24" version="1">MDSATLGTGYSSTLTAGFSKWRNPSPIPRSIQFVRHQQNKRSLLFKSLKCVLTSHPSLAAVRLRSLASEFTELTEPIDRVKRLLHYAELLPPFDESARVPANRVTGCTAEVWLDVSLDEFGGTRFAVDSDSEITKGFCSCLIRILDGAAPEEVLKVKAEDLMEMNVGVGMGVRAHSRVNAWHNILTSMQKRTEALVAERERRAEPQTQVVSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YGH4</accession>
    <name>A0AA38YGH4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">LOB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_026521</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LOB domain-containing protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YGH4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004883">
        <property type="entry name" value="LOB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31301:SF68">
        <property type="entry name" value="LOB DOMAIN-CONTAINING PROTEIN 32-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31301">
        <property type="entry name" value="LOB DOMAIN-CONTAINING PROTEIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03195">
        <property type="entry name" value="LOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50891">
        <property type="entry name" value="LOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LOB" evidence="2">
        <location>
            <begin position="6"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9670008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="246" mass="27258" checksum="7C8C35B95A617377" modified="2024-01-24" version="1">MSSSNSPCAACKLQRRKCTQECIYAPYFPPDQPHKFANVHKVFGASNVAKLLNEINVVHREDAVNSLAYEAEARLHDPVYGCVGLISVLQQTLKQVQSDLLAAKKELSAYIGPIAMQPNFQHPGYMPQQHMTSPSSSSVIPYNMSPMLGLPTGASHGGQLVIREPQPQQSQQYQHQRHNILEAQQMAAREQQEMLRTFEQQQRQQELIRFNSGFEEAGTVGAGGFSQTTAATMVPSLALGKFDNPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YHU4</accession>
    <name>A0AA38YHU4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_026947</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YHU4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045191">
        <property type="entry name" value="MBR1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22937:SF224">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MBR1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22937">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF165"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="100"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9670723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="153" mass="17497" checksum="2993D78366D68054" modified="2024-01-24" version="1">MGLQGSGGTIQYRSSHTRFPGYWRLLDVIYLPDENEDDEDDEDDDMHLDIDNMSYEELVVLGERIGNVSTGFSNEEIMARLKQRKYTCLATGPWVGEETCCVCQEYADKDDVGKLDCEHEYHVACIREWLAQNSCPICKKTALAPRESNPHQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YJ57</accession>
    <name>A0AA38YJ57_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_025220</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YJ57"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041340054" evidence="2">
        <location>
            <begin position="27"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9671412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="59" mass="6390" checksum="D6EA8B38AFAEE8AA" modified="2024-01-24" version="1">MAKSSSISLIFILVLVLCLKKGEVSSMEESFDLASLPKGPTPYSSSRDKLTSNIKSIQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YLX3</accession>
    <name>A0AA38YLX3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DYW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_026213</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. PCMP-H subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YLX3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099402">
        <property type="term" value="P:plant organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000158">
        <property type="entry name" value="pentatricopeptide repeat-containing protein At2g33680"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032867">
        <property type="entry name" value="DYW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF382">
        <property type="entry name" value="PENTACOTRIPEPTIDE-REPEAT REGION OF PRORP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14432">
        <property type="entry name" value="DYW_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="2"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="34"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="DYW" evidence="4">
        <location>
            <begin position="193"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9672867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="264" mass="29896" checksum="32A0092C0388755A" modified="2024-01-24" version="1">MDTVSWNSMLKAYAMHGQGKEALQLFSRMDAQPDGATFVALLSACSHAGMVEEGAKIFETMSNNHGIVPQLDHYACMVDILGRAGRISKAKELINKMPIEPDSVVWSALLGGCRKHGETKFAKLATVKLKELDPNNSLGYVLMSNIFCTDGRFNEARLIKMEMEGKTVRKEPGLSWIEVGNQVHEFASGGQYLALHDIEDEHKEEQLYYHSEKLALIMKNIQICVDCHNFMKLASELVDMEIVVRYSNRFHHFKVKVCSYNDYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YMB2</accession>
    <name>A0AA38YMB2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TTI1 N-terminal TPR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_026359</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9673048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YMB2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052587">
        <property type="entry name" value="TELO2-interacting_protein_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057566">
        <property type="entry name" value="TPR_TTI1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049362">
        <property type="entry name" value="TTI1_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18460">
        <property type="entry name" value="TEL2 INTERACTING PROTEIN 1 TTI1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18460:SF3">
        <property type="entry name" value="TELO2-INTERACTING PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24173">
        <property type="entry name" value="TPR_TTI1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21547">
        <property type="entry name" value="TTI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TTI1 N-terminal TPR" evidence="2">
        <location>
            <begin position="225"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9673048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="992" mass="109696" checksum="89C23D801545A246" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YNU5</accession>
    <name>A0AA38YNU5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Mitochondrial adenine nucleotide transporter BTL3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_023401</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Probable mitochondrial adenylate carrier that catalyzes the transport of ATP, ADP and AMP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YNU5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000098">
        <property type="entry name" value="Mitochondrial substrate carrier family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24089">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="147"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="240"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="354"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00054707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9673828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="443" mass="48388" checksum="176CC9EF7EA034D5" modified="2024-01-24" version="1">MPGLEVWFRDWISHEQSQILVSDPDSVLFANGGLFLEPTIPSSFVHLISLRSASTGVSSSFDCSSVHRRKQVVLGGFRRERGVPEAFGGGFLSVSLRSDGFEQESTGCLVRNGDKGSEEAARFQGGNVVVEEGEKQDEVRVGATGAMNTTKHLWAGAVAAMVSRTFVAPLERLKLEYIVRGEQKHLFELIKTIACSQGLRGFWKGNFVNILRTAPFKAVNFYAYDTYRKQLLKFSGNEETTNFERFIAGAAAGITATILCLPLDTIRTKMVAPGGEALGGVIGTFRHMIQTEGFFSLYKGLVPSIISMAPSGAVFYGVYDILKSAYLHSPEGRKRIQNMSQQGKELNALDQLELGPVRTLLYGAVAGACAEAATYPFEVVRRQLQLQVQATKLSALATCVKIVEHGGVPALYAGLIPSLLQVLPSASISYFVYEFMKIFLKVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YPV6</accession>
    <name>A0AA38YPV6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Alpha/beta hydrolase fold-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_023794</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDXG' lipolytic enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YPV6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050466">
        <property type="entry name" value="Carboxylest/Gibb_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23024">
        <property type="entry name" value="ARYLACETAMIDE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23024:SF577">
        <property type="entry name" value="CARBOXYLESTERASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="3">
        <location>
            <begin position="121"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9674460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="365" mass="40177" checksum="D51C1B3E83C8691A" modified="2024-01-24" version="1">MNSPLFTSCRNYSPIGYKLILNLQNAIGLLLYAVVPVAFFKSSQFFMDSSKPKVIHEVVPYLRVYEDGTIERLLGTEVTPAAFDPQTGVVSTDVVVVPETGVSARLYRPKLTPNNQKLPLMVYFHGGAFCISSAADPKYHHSLNTLVATANVIAVSVNYRLAPEHPLPAAYDDSWAALQWVASHSSGGEGSEAWIRDDVDFDRVFLVGDSAGANIAHHLALRIVGSRSAQRMKLVGIGLIHPYFWGEDPIGSEAKDPVRKAMVDKWWQLVCPSGRGNDDPLINPFVDGAPSFKDLGCDKVLVCVAERDILRDRGRLYYETLVKSGWGGTAEMVETEGEDHVFHIFQVDSDKARSLVKSVGSFINH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YPZ0</accession>
    <name>A0AA38YPZ0_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_023763</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YPZ0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042752">
        <property type="term" value="P:regulation of circadian rhythm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009409">
        <property type="term" value="P:response to cold"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044678">
        <property type="entry name" value="COR27/28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33676">
        <property type="entry name" value="COLD REGULATED PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33676:SF3">
        <property type="entry name" value="COLD-REGULATED PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9674413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="258" mass="29073" checksum="8A402F3C3DFD6368" modified="2024-01-24" version="1">MESFRTRQTSGLTGGDSTEFVDQEETSSLGSPVTESMSMEWTDEKHSLYLKSMEASFVDQLYNSFGLLGWHSQKENPSDSKPSLQMLCKTRNSSGQFKVLRGGFWQKINFERARKQMVKADGSHVLLANPWIQHFRSACRDETVVLPALQENGASTRKAVHSRGKKASPCGLATTSKQFPMYHCHLRQRDSLSTNTEVSDQNFLDDDVEGGRESSLVSAKRMKTIVVDTCVNDQVLCLTVSIILLYSVHHNIMWFSIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YQA4</accession>
    <name>A0AA38YQA4_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_023929</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YQA4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34461">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34461:SF2">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="495"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="694"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="508"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9674691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="908" mass="100082" checksum="7B662450CA3CAD4F" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YQG3</accession>
    <name>A0AA38YQG3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BFN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_023985</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Bifunctional nuclease with both RNase and DNase activities. Involved in basal defense response. Participates in abscisic acid-derived callose deposition following infection by a necrotrophic pathogen.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bifunctional nuclease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YQG3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030891">
        <property type="term" value="C:VCB complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.690.10">
        <property type="entry name" value="Bifunctional nuclease domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036104">
        <property type="entry name" value="BFN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003729">
        <property type="entry name" value="Bi_nuclease_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15160:SF3">
        <property type="entry name" value="BIFUNCTIONAL NUCLEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15160">
        <property type="entry name" value="VON HIPPEL-LINDAU PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02577">
        <property type="entry name" value="BFN_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103256">
        <property type="entry name" value="Hypothetical protein TM0160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51658">
        <property type="entry name" value="BFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BFN" evidence="4">
        <location>
            <begin position="122"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9674781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="327" mass="36489" checksum="0A1603B986764668" modified="2024-01-24" version="1">MGTLQGPVICPTVRAKQAGVYTLPVSLPLKKTKIFRSGFWGFRGNCGGRTKVGAVPHQLHTRKCKTLQCSFSSSSDGNGSMAENFNENDEDYVNSSVVEAVEVKSGADGFMIKMRDGRHLRCVHNNPQGGHMPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAVRNVQIARPTLYQVMKEMIDKMGYAVKLVRVTKRVHEAYFAQLYLTKVGNEKESVSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRVIESAKVSVQASSSDGLLFTELDRPSGQPCIETKEFDLVRNMLIAAVEERYRDAAQWRDKLTQFRSKRNWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YQV3</accession>
    <name>A0AA38YQV3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myb/SANT-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_024074</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YQV3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045026">
        <property type="entry name" value="LIMYB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024752">
        <property type="entry name" value="Myb/SANT-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47584">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47584:SF2">
        <property type="entry name" value="L10-INTERACTING MYB DOMAIN-CONTAINING PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12776">
        <property type="entry name" value="Myb_DNA-bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb/SANT-like" evidence="2">
        <location>
            <begin position="43"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="220"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9674944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="336" mass="37898" checksum="92B0DEBC0FCCB797" modified="2024-01-24" version="1">MLVTRYYSFHLYTVVCFSKSYDAKVEMDGEANHQPKQERLRTRWTASLDKIFADLVVKQIQLGNRPNNVFDKKTWNRIRDEFNVQTDLNFNNNQLRKHLDVLRTRYYNLKSAFDQNEYAMDDSCCIGFDLWEDIGPRPDTIKIKDCPIYDQLCMIFTESGADGKYAQSSHYEELDDKSVGIGTAIGTPGLTSCPESGKLSSKPLSSSKIVQENASLAEKLDKKTAERKRKRSSETGSFSGQNKGGKGINDVMAEAMLEMVAASKLRTVTSIQTDGKYTITNCIKALDEIEGIDQKIYFAALDLFEDPNLRETFISLGSNETRLTWLLGKCGNATSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YTE5</accession>
    <name>A0AA38YTE5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Sucrose-phosphate synthase</fullName>
            <ecNumber evidence="4 8">2.4.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_025058</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Plays a role in photosynthetic sucrose synthesis by catalyzing the rate-limiting step of sucrose biosynthesis from UDP-glucose and fructose- 6-phosphate. Involved in the regulation of carbon partitioning in the leaves of plants. May regulate the synthesis of sucrose and therefore play a major role as a limiting factor in the export of photoassimilates out of the leaf. Plays a role for sucrose availability that is essential for plant growth and fiber elongation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>beta-D-fructose 6-phosphate + UDP-alpha-D-glucose = sucrose 6(F)-phosphate + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22172"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:57723"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.4.1.14"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Glycan biosynthesis; sucrose biosynthesis; sucrose from D-fructose 6-phosphate and UDP-alpha-D-glucose: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 8">Homodimer or homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the glycosyltransferase 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.14" evidence="4 8"/>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9676351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YTE5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046524">
        <property type="term" value="F:sucrose-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005986">
        <property type="term" value="P:sucrose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03800">
        <property type="entry name" value="GT4_sucrose_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16419">
        <property type="entry name" value="HAD_SPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1070.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006380">
        <property type="entry name" value="SPP-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044161">
        <property type="entry name" value="SPS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035659">
        <property type="entry name" value="SPS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012819">
        <property type="entry name" value="SPS_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000368">
        <property type="entry name" value="Sucrose_synth_GT-B1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02468">
        <property type="entry name" value="sucrsPsyn_pln"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46039">
        <property type="entry name" value="SUCROSE-PHOSPHATE SYNTHASE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46039:SF1">
        <property type="entry name" value="SUCROSE-PHOSPHATE SYNTHASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00862">
        <property type="entry name" value="GT-B_Sucrose_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05116">
        <property type="entry name" value="S6PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sucrose synthase first GT-B" evidence="11">
        <location>
            <begin position="192"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="10">
        <location>
            <begin position="495"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="domain" description="Sucrose phosphatase-like" evidence="12">
        <location>
            <begin position="804"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="136"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="136"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="154"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU368006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF05116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9676351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1043" mass="118088" checksum="D7005D67194461AF" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YVE3</accession>
    <name>A0AA38YVE3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transcription termination factor MTERF15, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_022319</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the mTERF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YVE3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.70.10:FF:000030">
        <property type="entry name" value="Transcription termination factor MTERF15 mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.70.10">
        <property type="entry name" value="Transcription termination factor 3, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003690">
        <property type="entry name" value="MTERF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038538">
        <property type="entry name" value="MTERF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13068">
        <property type="entry name" value="CGI-12 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13068:SF23">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR MTERF15, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02536">
        <property type="entry name" value="mTERF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00733">
        <property type="entry name" value="Mterf"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0806">Transcription termination</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9677252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="439" mass="50575" checksum="791A3CA01B258EC7" modified="2024-01-24" version="1">MAIRVLARPAVHNLKSIVAFQTPKPQSLSTVNQIPVSPKISPQSPYRKQISLFNLFQRHGFPPSQLHGFLKKNQFFQNCNLLELEKSLGILFSFQIPQKFILSLISDCPRLLEFEFLKKWEMGIAKLGVSGVSPFELDPDDVSRCVKVLKGLGFSDGTVDRILEEFPRVIMSNESEIQRKIQFLLGIGFPESGIDGIFHSLPGILGLGIEDRLEPLLDEFGKLGFSEDVVRREISREPRMLGMELGEMSRCLELLGTLKCRVPIKEKIFREGALRAGFEVKLRVDCLCRYGLIRREAFEVLWKEPRVIIYEIEDIEEKIEFLVHRMRYNVGCLIEVPEYLGVNFDKQIVSRWNVIDYLRSKGGLGCKVGLKGLIKPSRLRFYNLYVKPYPECEKLFGRFSRDVEVRNRHPVGLWKLMKPQKHPESKDDVKNMKCFVESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YVF0</accession>
    <name>A0AA38YVF0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_022414</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YVF0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000120">
        <property type="entry name" value="Alpha/beta-Hydrolases superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47914">
        <property type="entry name" value="ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47914:SF1">
        <property type="entry name" value="ALPHA_BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9677416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="377" mass="41648" checksum="9660A4597B4D8476" modified="2024-01-24" version="1">MPSLMASSAILHFSPSSSLLSRKTCRPSVFTAAHFPRSLTIKASTSLDYSNVSVDDKLAAPSKTNNWKWKFDDNSINIYYEEYEKESSEPPKDILMIPTISDVSTVEEWRLVARDIVQRVGKVNWRATIIDWPGLGFSDRPKIDYNADVLEKFLVDFMSAPDCPISQTKNDLVVFGGGHAATITIRAAKKGLVKPAAIAAVAPTWAGPLPIVFGRDSDMETRYGLLRGTLRAPAVGWMMYNMLVSNEKAIQSQYKSHVYADPNNVTPSIVESRYALTKRKGARYVPAAFLTGLLDPVNSREEFLELFAALEGQIPVLVVSTKGSPKRSKAEMEALRGAKGVSKFVELPGALLPQEEYPAVVAEELYGFLQENFECEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YVZ9</accession>
    <name>A0AA38YVZ9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HhH-GPD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_022562</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YVZ9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47203">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47203:SF1">
        <property type="entry name" value="HYPOTHETICAL BASE EXCISION DNA REPAIR PROTEIN (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HhH-GPD" evidence="2">
        <location>
            <begin position="112"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9677644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="231" mass="25302" checksum="F3450975DA590F1D" modified="2024-01-24" version="1">MQRSRKRKQEESSSCSKESATKSARNDVVIDPYPSHPRPTPVECRAVRDDLLALHGFPQRFEKYRKLRLPPLPHTSSPGLDGGGGTPVKLDPSDGDDVNGSSQKESVLDGLVSIILSQNTTDVNSQRAFASLKSAFPTWQDVLAADSKSIENAIRCGGLAVTKASCIKKMLSCLLERKGKLCLEYLRDLTVDEIKTELSHFKGIGPKTVILGGNMEFHSVVYFLDGLEGEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YWG6</accession>
    <name>A0AA38YWG6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 10">Adenosylhomocysteinase</fullName>
            <ecNumber evidence="6 10">3.13.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_022757</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 10">
            <text>S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine</text>
            <dbReference type="Rhea" id="RHEA:21708"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16335"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
            <dbReference type="EC" id="3.13.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9 10">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
        <text evidence="9 10">Binds 1 NAD(+) per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the adenosylhomocysteinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.13.2.1" evidence="6 10"/>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YWG6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004013">
        <property type="term" value="F:adenosylhomocysteinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033353">
        <property type="term" value="P:S-adenosylmethionine cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00401">
        <property type="entry name" value="SAHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000004">
        <property type="entry name" value="Adenosylhomocysteinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1480">
        <property type="entry name" value="Adenosylhomocysteinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00563">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042172">
        <property type="entry name" value="Adenosylhomocyst_ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000043">
        <property type="entry name" value="Adenosylhomocysteinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015878">
        <property type="entry name" value="Ado_hCys_hydrolase_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020082">
        <property type="entry name" value="S-Ado-L-homoCys_hydrolase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00936">
        <property type="entry name" value="ahcY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004005">
        <property type="entry name" value="PRK05476.2-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23420">
        <property type="entry name" value="ADENOSYLHOMOCYSTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23420:SF29">
        <property type="entry name" value="ADENOSYLHOMOCYSTEINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05221">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00670">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001109">
        <property type="entry name" value="Ad_hcy_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00996">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00997">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00738">
        <property type="entry name" value="ADOHCYASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00739">
        <property type="entry name" value="ADOHCYASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 9 10" id="KW-0520">NAD</keyword>
    <keyword evidence="3 10" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S-adenosyl-L-homocysteine hydrolase NAD binding" evidence="12">
        <location>
            <begin position="240"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="206"/>
            <end position="208"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="271"/>
            <end position="276"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="348"/>
            <end position="350"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="397"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="404"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001109-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001109-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9677970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="485" mass="53164" checksum="791BC5D301E28C7A" modified="2024-01-24" version="1">MALVVEKTTSGREYKVKDMSQADFGRLEIELAEVEMPGLMACRTEFGPQQPFKGARITGSLHMTIQTAVLIESLTALGAEVRWCSCNIFSTQDHAAAAIARDSAAVFAWKGETLQEYWWCTERALDWGPGGGPDLIVDDGGDATLLIHEGVKAEEEYERSGKLPDPASTDNAEFQIVLGIIKEGLSVDPCKYRKMKERLVGVSEETTTGVKRLYQMQANGTLLFPAINVNDSVTKSKFDNLYGCRHSLPDGLMRATDIMIAGKVAVVCGYGDVGKGCAAALKQAGAQVIVTEIDPICALQALMEGIPVLTLEDVVSKADIFVTTTGNKDIVMVHHMKQMKNNAIVCNIGHFDNEIDMLGLETYPGVKRITIKPQTDRWVFPETNSGIIILAEGRLMNLGCATGHPSFVMSCSFTNQVIAQLELWKEKATCKYEKKVYVLPKHLDEKVAALHLGKLGAKLTKLSPEQAAYINVPVEGPYKPTHYRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YWX1</accession>
    <name>A0AA38YWX1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">dolichyl-diphosphooligosaccharide--protein glycotransferase</fullName>
            <ecNumber evidence="7">2.4.99.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">PVL29_022949</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="23">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a di-trans,poly-cis-dolichyl diphosphooligosaccharide + L-asparaginyl-[protein] = N(4)-(oligosaccharide-(1-&gt;4)-N-acetyl-beta-D-glucosaminyl-(1-&gt;4)-N-acetyl-beta-D-glucosaminyl)-L-asparaginyl-[protein] + a di-trans,poly-cis-dolichyl diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22980"/>
            <dbReference type="Rhea" id="RHEA-COMP:12804"/>
            <dbReference type="Rhea" id="RHEA-COMP:12805"/>
            <dbReference type="Rhea" id="RHEA-COMP:19506"/>
            <dbReference type="Rhea" id="RHEA-COMP:19509"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:50347"/>
            <dbReference type="ChEBI" id="CHEBI:57497"/>
            <dbReference type="ChEBI" id="CHEBI:57570"/>
            <dbReference type="ChEBI" id="CHEBI:132529"/>
            <dbReference type="EC" id="2.4.99.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the oligosaccharyltransferase (OST) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the STT3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.99.18" evidence="7"/>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9678210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YWX1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004579">
        <property type="term" value="F:dolichyl-diphosphooligosaccharide-protein glycotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12610:FF:000002">
        <property type="entry name" value="dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003674">
        <property type="entry name" value="Oligo_trans_STT3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048999">
        <property type="entry name" value="STT3-PglB_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048307">
        <property type="entry name" value="STT3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13872">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13872:SF1">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT STT3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02516">
        <property type="entry name" value="STT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21436">
        <property type="entry name" value="STT3-PglB_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="17" id="KW-0464">Manganese</keyword>
    <keyword evidence="15 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="10 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="170"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="200"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="231"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="266"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="297"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="327"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="390"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="415"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="domain" description="Oligosaccharyl transferase STT3 N-terminal" evidence="21">
        <location>
            <begin position="49"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="domain" description="STT3/PglB/AglB core" evidence="22">
        <location>
            <begin position="564"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF02516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF21436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAJ9678210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="741" mass="83365" checksum="CD68646A557FAB7D" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YX19</accession>
    <name>A0AA38YX19_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_022851</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9678088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YX19"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47553">
        <property type="entry name" value="MYOSIN-11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47553:SF1">
        <property type="entry name" value="RING_FYVE_PHD ZINC FINGER SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="59"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9678088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="99" mass="10809" checksum="27A943CF0ED00834" modified="2024-01-24" version="1">MVYADKKAHISEANLGQAFTSRKNKSSLQQEILAHKRKAASLKREGKLAEAYDPQPRFSPSDASISSSSVTPTGQKAQTSVNSAPKKKTLSSCERFKLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YZ26</accession>
    <name>A0AA38YZ26_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA mismatch repair protein MSH3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4 12">DNA mismatch repair protein MSH3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">MutS protein homolog 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_021318</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Component of the post-replicative DNA mismatch repair system (MMR).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA mismatch repair MutS family. MSH3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YZ26"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006312">
        <property type="term" value="P:mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03287">
        <property type="entry name" value="ABC_MSH3_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.110:FF:000010">
        <property type="entry name" value="DNA mismatch repair protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1170.10:FF:000004">
        <property type="entry name" value="DNA mismatch repair protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1420.10:FF:000004">
        <property type="entry name" value="DNA mismatch repair protein Msh3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002130">
        <property type="entry name" value="DNA mismatch repair protein MSH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1420.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.10">
        <property type="entry name" value="DNA repair protein MutS, domain I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.110">
        <property type="entry name" value="MutS, connector domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007695">
        <property type="entry name" value="DNA_mismatch_repair_MutS-lik_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017261">
        <property type="entry name" value="DNA_mismatch_repair_MutS/MSH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007861">
        <property type="entry name" value="DNA_mismatch_repair_MutS_clamp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007696">
        <property type="entry name" value="DNA_mismatch_repair_MutS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016151">
        <property type="entry name" value="DNA_mismatch_repair_MutS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036187">
        <property type="entry name" value="DNA_mismatch_repair_MutS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007860">
        <property type="entry name" value="DNA_mmatch_repair_MutS_con_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036678">
        <property type="entry name" value="MutS_con_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361:SF122">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MSH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MUTS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01624">
        <property type="entry name" value="MutS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05188">
        <property type="entry name" value="MutS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05192">
        <property type="entry name" value="MutS_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05190">
        <property type="entry name" value="MutS_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037677">
        <property type="entry name" value="DNA_mis_repair_Msh6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00533">
        <property type="entry name" value="MUTSd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55271">
        <property type="entry name" value="DNA repair protein MutS, domain I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53150">
        <property type="entry name" value="DNA repair protein MutS, domain II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48334">
        <property type="entry name" value="DNA repair protein MutS, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00486">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 13" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9 13" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair proteins mutS family" evidence="14">
        <location>
            <begin position="766"/>
            <end position="782"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00073774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS00486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9679350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="977" mass="107205" checksum="E555ED2CE274B127" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z0N6</accession>
    <name>A0AA38Z0N6_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_019496</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z0N6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053258">
        <property type="entry name" value="Ca-permeable_cation_channel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34115">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34115:SF5">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="120"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9680208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="174" mass="19364" checksum="D54BD473290254B7" modified="2024-01-24" version="1">MSTSNVNGLGINLHYQHHHNSAPSYPILALLTFILPVLLVLIYFKFQNESTSVFQAHPITIKVVVANLLAFALAFGIEFTFHPCCPSTTCAAFLRTTILFCGSLSVASVASILFPDAWRPLIYLIYALLSLANLHGLVREWCGWVHHGLMEKLQAVCTGIQQWYVRRSAPLLPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z100</accession>
    <name>A0AA38Z100_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glyceraldehyde-3-phosphate dehydrogenase (phosphorylating)</fullName>
            <ecNumber evidence="3">1.2.1.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_019577</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.12" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z100"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004365">
        <property type="term" value="F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020831">
        <property type="entry name" value="GlycerAld/Erythrose_P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020829">
        <property type="entry name" value="GlycerAld_3-P_DH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020828">
        <property type="entry name" value="GlycerAld_3-P_DH_NAD(P)-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10836">
        <property type="entry name" value="GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10836:SF112">
        <property type="entry name" value="GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE GAPC1, CYTOSOLIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02800">
        <property type="entry name" value="Gp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00078">
        <property type="entry name" value="G3PDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00846">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyceraldehyde 3-phosphate dehydrogenase NAD(P) binding" evidence="7">
        <location>
            <begin position="2"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9680299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="106" mass="11536" checksum="6100EDC27BF1390F" modified="2024-01-24" version="1">MSKLVWVIRIYKDKAAAHLKVGAEKAIISAPNKDAPIFVAGVNEKEYKPDIDIVSNASCITNCLVPLAKVINDRSGIVEGLLTTIHLITATQKTVDSLRDENAQHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z1B3</accession>
    <name>A0AA38Z1B3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">VASt domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_019774</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z1B3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043069">
        <property type="term" value="P:negative regulation of programmed cell death"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13220">
        <property type="entry name" value="PH-GRAM_GRAMDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000008">
        <property type="entry name" value="GRAM domain containing 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004182">
        <property type="entry name" value="GRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031968">
        <property type="entry name" value="VASt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47666">
        <property type="entry name" value="PROTEIN VASCULAR ASSOCIATED DEATH 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47666:SF1">
        <property type="entry name" value="PROTEIN VASCULAR ASSOCIATED DEATH 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02893">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16016">
        <property type="entry name" value="VASt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00568">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51778">
        <property type="entry name" value="VAST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="539"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="domain" description="VASt" evidence="8">
        <location>
            <begin position="280"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="199"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="474"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="598"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="7"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="22"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="211"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="474"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9680556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="639" mass="73035" checksum="EE941A03C4A36B0E" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z1G4</accession>
    <name>A0AA38Z1G4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Chalcone/stilbene synthase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_019833</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thiolase-like superfamily. Chalcone/stilbene synthases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z1G4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030639">
        <property type="term" value="P:polyketide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012328">
        <property type="entry name" value="Chalcone/stilbene_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011141">
        <property type="entry name" value="Polyketide_synthase_type-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11877">
        <property type="entry name" value="HYDROXYMETHYLGLUTARYL-COA SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11877:SF46">
        <property type="entry name" value="TYPE III POLYKETIDE SYNTHASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02797">
        <property type="entry name" value="Chal_sti_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Chalcone/stilbene synthase C-terminal" evidence="3">
        <location>
            <begin position="7"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9680631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="73" mass="8219" checksum="B20B9D22E6C09286" modified="2024-01-24" version="1">MLAAGRDTLKLRSEKLECSRRALMDYGNVSSNTIFYVMEYMREGLKRKGGEEWGLALAFGPGITFEGILIRSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z1G8</accession>
    <name>A0AA38Z1G8_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_019812</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z1G8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10747">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10719">
        <property type="entry name" value="DnaJ_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.20:FF:000068">
        <property type="entry name" value="Chaperone protein dnaJ 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000041">
        <property type="entry name" value="Chaperone protein DNAj, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.230.10:FF:000001">
        <property type="entry name" value="DnaJ subfamily A member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.20:FF:000003">
        <property type="entry name" value="DnaJ subfamily A member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.230.10">
        <property type="entry name" value="Heat shock protein DnaJ, cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.260.20">
        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01152">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012724">
        <property type="entry name" value="DnaJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044713">
        <property type="entry name" value="DNJA1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001305">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43888">
        <property type="entry name" value="DNAJ-LIKE-2, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00684">
        <property type="entry name" value="DnaJ_CXXCXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="DnaJ/Hsp40 cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="HSP40/DnaJ peptide-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51188">
        <property type="entry name" value="ZF_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="J" evidence="7">
        <location>
            <begin position="13"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="CR-type" evidence="8">
        <location>
            <begin position="138"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CR-type" evidence="5">
        <location>
            <begin position="138"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="369"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9680610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="422" mass="47101" checksum="E2A45312B3AD5AA6" modified="2024-01-24" version="1">MFGRPPRRSDNTKYYDVLGVSKNANQDELKKAYKKAAIKNHPDKGGDPEKFKELSQAYDVLSDPDKREIYDQYGEDGLKEGMGGGGGGGGFHNPVDIFESFFGGGAFGGGGSSRGRRQKRGEDVVHTLKVSLEDLYNGTSKKLSLSRNVLCPKCKGKGSKSGASGRCYGCQGSGMKITTRQIAPGMIQQMQHVCHECRGTGEVISERDRCPQCKGNKVSTEKKVLEVHVEKGMQHGERIVFHGEADQAPDTTTGDIVFVLQLKDHAKFKRKYDDLYVEHTLSLTEALCGFQFALTHLDGRQLLIKSNPGEIIKPDQYKAINDEGMPHHQRPFMKGKLYIHFDVEFPESGILSPDQCKALESILPQKRSKQTSAMEVDDAEETTLYDVNIEEEMRRKQQQQQHEAYDEDDEDFGAPRVQCAQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z1N7</accession>
    <name>A0AA38Z1N7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ripening-related protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_019607</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the kiwellin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z1N7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd22270">
        <property type="entry name" value="DPBB_kiwellin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039271">
        <property type="entry name" value="Kiwellin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33191:SF58">
        <property type="entry name" value="RIPENING-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33191">
        <property type="entry name" value="RIPENING-RELATED PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24300">
        <property type="entry name" value="KWL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041286177" description="Ripening-related protein 2" evidence="5">
        <location>
            <begin position="27"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9680339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="188" mass="20259" checksum="B1DD57911D220D27" modified="2024-01-24" version="1">MKTFGLKASLLLIAILVFTDCLNVEGQVCHPSGKIRGKKPPPGECNQGNDSDCCKEGKLYTTYKCSPTASSHTKAYLTLNGFEKDEDGGAPSECDNQYHSDDTPVVALSTGWYNKGSRCLNNITITANGRSVVAMVVDECDSTMGCDSDHDYQPPCANNIVDASRAVWKALGVPQDQWGGLDITWTNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z203</accession>
    <name>A0AA38Z203_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_020043</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z203"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055336">
        <property type="entry name" value="At4g00755-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39741">
        <property type="entry name" value="F-BOX DOMAIN CONTAINING PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39741:SF14">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9680916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="396" mass="45265" checksum="5220200CEFB15289" modified="2024-01-24" version="1">METREDFFNWLDLDVIMVILMCLGDPADFIRVSSLSRFWYNFVIANGLCKELCLRMFPQLSAVDSVVERGNRRRNPIDVGSCNSAEWESLEREHRAFAFLARQCKTLPATSCIADAVCASSTDNYPFESIVNTLERGDRVGPRASYWSSKGQKDHTVPETLVYKLDADFCLITEINVQPFQAFFQTGDPIYAAKSVRFRVGRPKSPSPVDISHQEAIQRNISTDTSEEFPMAQPVDILHQEAPEDRFIWTWTSQEFPMAQENRLQKFKLPKPILCFGGIVQIELLGRVQRQEMDGLFYICVSHVEVIGGRLFPGFDIEILEPSGKIVLKYFPNLSPIRIRHRRAQPFLIPRLPLLGGGLMQGPRRAFGRMPHIFRLIRQQDESDDPGSQQHEPEPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z2B4</accession>
    <name>A0AA38Z2B4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">S-acyltransferase</fullName>
            <ecNumber evidence="8">2.3.1.225</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Palmitoyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_020115</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="8">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="8"/>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9681061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z2B4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12246">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="54"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="212"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="9">
        <location>
            <begin position="129"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9681061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="308" mass="34854" checksum="0EF57D95FEC0A7C2" modified="2024-01-24" version="1">MDINLFKFCSSLKALGYLMILMVAAIIVVSYYAVVVVTWGPKLLVGGLDSVLSFAIIVVFHILLILLTWCYFMVVFRDPGSVPENWRPVSEEYNLEEGPMTSSDCVVPETLNSTWSSSDGQERRPAVGYCIQCQNGKPPRCHHCSVCQRCVLKMDHHCVWVVNCVGAYNYKFFLLFLLYTFLETTLDTLALLPSFINFFGEAKNHSASPGNLSIIFLAFVINLAFALSLLCFIVMHVSLLSSNTTSIEVYEKRRAVRWKYDLGRKTNFEQVFGKKKALWLFPLYSEDDFSSIPALHGLDFPTRSDVEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z2U9</accession>
    <name>A0AA38Z2U9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_020057</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z2U9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000270">
        <property type="entry name" value="Alpha/beta-Hydrolases superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45763">
        <property type="entry name" value="HYDROLASE, ALPHA/BETA FOLD FAMILY PROTEIN, EXPRESSED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45763:SF54">
        <property type="entry name" value="HYDROLASE, ALPHA_BETA FOLD FAMILY PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="54"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9680954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="327" mass="36770" checksum="5FFAF16DBF0254E1" modified="2024-01-24" version="1">MATFLGKVVQPQISNLCGSLGGPPVTALRIRLKDGRSLAYSETGVPRDKAEFKIILAHGFTGSRLDLLRASPEIIEEMGIYMVCYDRAGHGESDPNPRRWLGSEASDVEELADALELGKKFYVVGTSMGGYVAWACLKYIPDRLAGAALLAPVINYRWPGFPKDLSKEAYYQQAVGDQWLLRVAYYAPWLLNWWVNQSWLPSPTVIQGNTFLPNHLDSQFRDRAISSGIFLQRRNISTLQGEHESLHRDLMVMFGKWEFDPMDLPPPSFPVHLWQGCEDGIVPASLQKHISQRVGWIKYHEVPDGGHFLNAIPGFDDHLLKTLLLQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z3E9</accession>
    <name>A0AA38Z3E9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S ribosomal protein L36</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_020381</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL36 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9681482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z3E9"/>
    <dbReference type="SMR" id="A0AA38Z3E9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1760:FF:000001">
        <property type="entry name" value="60S ribosomal protein L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1760">
        <property type="entry name" value="60S ribosomal protein L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000509">
        <property type="entry name" value="Ribosomal_eL36"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038097">
        <property type="entry name" value="Ribosomal_eL36_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10114">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01158">
        <property type="entry name" value="Ribosomal_L36e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01190">
        <property type="entry name" value="RIBOSOMAL_L36E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="79"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="21"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="79"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9681482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="110" mass="12483" checksum="BA24B434B791C405" modified="2024-01-24" version="1">MAPKQPNTGLFVGLNKGHVVTKKELPPRPSDRKGKTSKRVHFVRNIIREVAGFAPYEKRITELLKVGKDKRALKVAKRKLGTHKRAKKKREEMSSVLRKMRSGGVGEKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z457</accession>
    <name>A0AA38Z457_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">IBH1-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_017895</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9681733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z457"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11444">
        <property type="entry name" value="bHLH_AtIBH1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044549">
        <property type="entry name" value="bHLH_AtIBH1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044660">
        <property type="entry name" value="IBH1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059002">
        <property type="entry name" value="IBH1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33124:SF81">
        <property type="entry name" value="TRANSCRIPTION FACTOR BHLH149-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33124">
        <property type="entry name" value="TRANSCRIPTION FACTOR IBH1-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26576">
        <property type="entry name" value="IBH1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="IBH1-like N-terminal" evidence="6">
        <location>
            <begin position="56"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="22"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF26576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9681733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="207" mass="23204" checksum="01A00A800DDD7EC5" modified="2024-01-24" version="1">MLWCLETTSSYSMEKSYMVTDSEGGYRSMESDRRKRKKMESGSQKVKRGGKWRTEAEQRIYSAKLVEAITKVRRSSAPAAPVHRGRAVREAADRVLAAAGKGRTRWSRAILTSRVGLRLRKRRKVTVTGNNRLRKPAVSKQEPPLKRRVRVLGRLVPGCRKLSFPNLLEEATDYIAALEMQVRAMTALAELLAGTGASSSNWQPPSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z4J5</accession>
    <name>A0AA38Z4J5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pre-rRNA-processing protein TSR2 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_018239</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TSR2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z4J5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019398">
        <property type="entry name" value="Pre-rRNA_process_TSR2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21250">
        <property type="entry name" value="PRE-RRNA-PROCESSING PROTEIN TSR2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10273">
        <property type="entry name" value="WGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="130"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="134"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9682266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="208" mass="23177" checksum="6C3E76D62A76D3C9" modified="2024-01-24" version="1">MDSGGVAPQLSEEATHHLQQGIVMLLSQWSALQMAVHNEWGGRQSSQLADQLALDIFSWFTHSKEPLYIDDLENILDEAMLSLNTMTEDGSIEEVAEKLMTMHEECLEGNYQSIEKLREASSRPVVHHIRQAVNEEDEDEDDDDDDDDENMGNDNSSNMIVDAPEAPPPNLNPADVPVEEPRASNPKAAEADDGWVVVASKRNRGKRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z4L5</accession>
    <name>A0AA38Z4L5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Acyl carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_018187</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 13">Carrier of the growing fatty acid chain in fatty acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the acyl carrier protein (ACP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z4L5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000036">
        <property type="term" value="F:acyl carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031177">
        <property type="term" value="F:phosphopantetheine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1200.10:FF:000017">
        <property type="entry name" value="Acyl carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01217">
        <property type="entry name" value="Acyl_carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003231">
        <property type="entry name" value="ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044813">
        <property type="entry name" value="ACP_chloroplastic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020806">
        <property type="entry name" value="PKS_PP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006162">
        <property type="entry name" value="Ppantetheine_attach_site"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00517">
        <property type="entry name" value="acyl_carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002148">
        <property type="entry name" value="PRK00982.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46153">
        <property type="entry name" value="ACYL CARRIER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46153:SF20">
        <property type="entry name" value="ACYL CARRIER PROTEIN 2, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00823">
        <property type="entry name" value="PKS_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00012">
        <property type="entry name" value="PHOSPHOPANTETHEINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="12 13" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5 13" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4 13" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0934">Plastid</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Carrier" evidence="14">
        <location>
            <begin position="59"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9682189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="139" mass="14828" checksum="C09DF807A396F543" modified="2024-01-24" version="1">MAAFAGAFVSVQPRPRLLRQSLATTSIPSLKSLSFSNRGGRLTSQHLPLRSRVSCAAKPETVEKVCAIVKKQLALPEDSAITGESKFAALGADSLDTVEIVMGLEEEFGINVEEESAQSIATVQDAADLIEKLIENKDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z7N0</accession>
    <name>A0AA38Z7N0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase</fullName>
            <ecNumber evidence="3">3.2.1.96</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_016430</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Endoglycosidase that releases N-glycans from glycoproteins by cleaving the beta-1,4-glycosidic bond in the N,N'-diacetylchitobiose core. Involved in the production of high-mannose type N-glycans during plant development and fruit maturation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>an N(4)-(oligosaccharide-(1-&gt;3)-[oligosaccharide-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc)-L-asparaginyl-[protein] + H2O = an oligosaccharide-(1-&gt;3)-[oligosaccharide-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-D-GlcNAc + N(4)-(N-acetyl-beta-D-glucosaminyl)-L-asparaginyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:73067"/>
            <dbReference type="Rhea" id="RHEA-COMP:12603"/>
            <dbReference type="Rhea" id="RHEA-COMP:18176"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:132248"/>
            <dbReference type="ChEBI" id="CHEBI:192714"/>
            <dbReference type="ChEBI" id="CHEBI:192715"/>
            <dbReference type="EC" id="3.2.1.96"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 85 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.96" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9683925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z7N0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033925">
        <property type="term" value="F:mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006491">
        <property type="term" value="P:N-glycan processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06547">
        <property type="entry name" value="GH85_ENGase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000043">
        <property type="entry name" value="cytosolic endo-beta-N-acetylglucosaminidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032979">
        <property type="entry name" value="ENGase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057882">
        <property type="entry name" value="ENGase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005201">
        <property type="entry name" value="TIM_ENGase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13246:SF1">
        <property type="entry name" value="CYTOSOLIC ENDO-BETA-N-ACETYLGLUCOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13246">
        <property type="entry name" value="ENDO BETA N-ACETYLGLUCOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03644">
        <property type="entry name" value="Glyco_hydro_85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25529">
        <property type="entry name" value="Ig_ENGASE1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytosolic endo-beta-N-acetylglucosaminidase TIM barrel" evidence="9">
        <location>
            <begin position="111"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="Cytosolic endo-beta-N-acetylglucosaminidase C-terminal" evidence="10">
        <location>
            <begin position="604"/>
            <end position="722"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00060018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF25529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9683925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="726" mass="81291" checksum="881E2CF1B0E0AD34" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z7N3</accession>
    <name>A0AA38Z7N3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Trichome birefringence-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_016433</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PC-esterase family. TBL subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9683931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z7N3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016413">
        <property type="term" value="F:O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029962">
        <property type="entry name" value="TBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026057">
        <property type="entry name" value="TBL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025846">
        <property type="entry name" value="TBL_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285:SF62">
        <property type="entry name" value="PROTEIN TRICHOME BIREFRINGENCE-LIKE 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285">
        <property type="entry name" value="PROTEIN TRICHOME BIREFRINGENCE-LIKE 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13839">
        <property type="entry name" value="PC-Esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14416">
        <property type="entry name" value="PMR5N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="Trichome birefringence-like N-terminal" evidence="9">
        <location>
            <begin position="108"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="Trichome birefringence-like C-terminal" evidence="8">
        <location>
            <begin position="162"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF14416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9683931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="453" mass="52540" checksum="9E715B37A905982A" modified="2024-01-24" version="1">MKPPFSSPTSSSALRKARFSPYLFTLLAFIVFVAVLYGEDFSCIVELGSESDGRPIGEFSSHNALTGAFFFFFPFFFFVLFQRLKYDFVVEKSREKLPFAIGEIEGGRCDVFSGMWVRDESNRPLYEESACPYIQPQLTCQEHGRPEKEYQFWRWQPHDCSLPNFNATLMLETLRGKRMMFVGDSLNRGQYVSMVCLLHSLIPEDAKSMETFDSLTIFTAKEYNATIEFYWAPFLLESNSDDAVIHRVSDRIVRKGSINKHGKYWKGVDILVFNTYLWWMTGLKMKILRGSFDDEVKDIVEITTEDAFRMAMKSMLKWVEKNMDPKKTRVFFTSMSPSHAKSIDWGGEAGKNCYNETTMIEDPTYWGSDCRKSVMEVIGEVFRKSKVPITFLNITQLSSYRRDAHTSIYKKQWSPLTPEQLANPVSYADCVHWCLPGLQDTWNELLFTKLFYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z9T1</accession>
    <name>A0AA38Z9T1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ARM repeat superfamily protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_016781</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z9T1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14873">
        <property type="entry name" value="OS06G0694100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14873:SF1">
        <property type="entry name" value="OS06G0694100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9684479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="446" mass="50262" checksum="5D32D1D43B043B2A" modified="2024-01-24" version="1">MAEALSALHDNLIQLSETIQESLSKAQYNPEEGTNVSVKSMLESLLPRKTSISNRTEGRRITEDQIQREIKDFSLCCAALASAQGSNHASLSWIPKGLSVTAVSAFEELSKAFSANLNAGKSTRIVELGVDCSLVSDDKKLVVELMPLVMPLLKASIKESSIDHLDDCDEVSAASARAPLAYAVVAAYQLRWFVTQVEQPHLGKLCALVIPCALTALDHWSPEVKGQGMISFIHLGKNVKAAEFSWYEDVILDTCCQNIASSDEIWHLVVEMSVLLVTCIHGSNPRSSWYERILNEMLNHLERQPKNKDRYTPWLQHIEPLFSSVGLVLLAHFRRIFPLFFQWMHSDNDQTLLLVLKRVYTVTKLTWIRNTPYVERLVDELAVTYKEAALRRAREEIRTHVIQILILLQQCKGLQFEAAWNKHRDDPNLTTLGPSFNTRNAAMVVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZB43</accession>
    <name>A0AA38ZB43_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">PGG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_017278</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZB43"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026961">
        <property type="entry name" value="PGG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24186:SF53">
        <property type="entry name" value="PGG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24186">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13962">
        <property type="entry name" value="PGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="447"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="487"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="521"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="553"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="107"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="155"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="PGG" evidence="10">
        <location>
            <begin position="441"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="417"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="424"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9685184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="598" mass="66230" checksum="524025CCE0BAC29C" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZCK8</accession>
    <name>A0AA38ZCK8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">WRKY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_015120</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZCK8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.80:FF:000004">
        <property type="entry name" value="WRKY transcription factor 65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.80">
        <property type="entry name" value="WRKY domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003657">
        <property type="entry name" value="WRKY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036576">
        <property type="entry name" value="WRKY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044810">
        <property type="entry name" value="WRKY_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32096:SF19">
        <property type="entry name" value="OS01G0750100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32096">
        <property type="entry name" value="WRKY TRANSCRIPTION FACTOR 30-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03106">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00774">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118290">
        <property type="entry name" value="WRKY DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50811">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="WRKY" evidence="7">
        <location>
            <begin position="75"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9686082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="240" mass="26080" checksum="C49B8976BF78BB0B" modified="2024-01-24" version="1">MDRRLHINPFINEQEEDPQGSPETAPDSPLSGEEAKLAATPSPKKSKRGVQKRVVSVPIGDVDGSRSKGEAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDPTVLIVTYACDHNHPLPPTKHHHSTAPAAVTATASTATPSPKFPPEEFAVFASQPDLELGGDSLLADGHFYWPPTSLAMLESPVFVGGYSAEDDVAVFAMGEEEESLFADLGELPECSLVFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZFG1</accession>
    <name>A0AA38ZFG1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">O-acyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_014041</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycerolipid metabolism; triacylglycerol biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Endoplasmic reticulum membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZFG1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009941">
        <property type="term" value="C:chloroplast envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004144">
        <property type="term" value="F:diacylglycerol O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027251">
        <property type="entry name" value="Diacylglycerol_acylTrfase1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004299">
        <property type="entry name" value="MBOAT_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014371">
        <property type="entry name" value="Oat_ACAT_DAG_ARE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10408:SF7">
        <property type="entry name" value="DIACYLGLYCEROL O-ACYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10408">
        <property type="entry name" value="STEROL O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03062">
        <property type="entry name" value="MBOAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000439">
        <property type="entry name" value="Oat_ACAT_DAG_ARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500231">
        <property type="entry name" value="Oat_dag"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="116"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="159"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="190"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="218"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="305"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="353"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="424"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="450"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="481"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR000439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000439-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9688095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="514" mass="58665" checksum="8F73971BADA4651D" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZGM7</accession>
    <name>A0AA38ZGM7_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_014370</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZGM7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9688681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="79" mass="8787" checksum="0CA97ADB8212D0A7" modified="2024-01-24" version="1">MMTLSSLRIPKLTRVPFMMSFPLGWKSYLVLQSVEIGVVDSPVVPMPKTGSPDSENPDESSYNVEHQIESHSGFPHAEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZJH2</accession>
    <name>A0AA38ZJH2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">HTH myb-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_012636</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MYB-CC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZJH2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000002">
        <property type="entry name" value="Myb family transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025756">
        <property type="entry name" value="Myb_CC_LHEQLE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006447">
        <property type="entry name" value="Myb_dom_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046955">
        <property type="entry name" value="PHR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01557">
        <property type="entry name" value="myb_SHAQKYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31499">
        <property type="entry name" value="MYB FAMILY TRANSCRIPTION FACTOR PHL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31499:SF83">
        <property type="entry name" value="MYB FAMILY TRANSCRIPTION FACTOR PHL7-LIKE ISOFORM X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14379">
        <property type="entry name" value="Myb_CC_LHEQLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="8">
        <location>
            <begin position="46"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="109"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="191"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="319"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="112"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="191"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9690077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="340" mass="37078" checksum="5223AA8B80E63E30" modified="2024-01-24" version="1">MYQPKAVPSPSLVHNNLLVHGQHSDCGANTMDPINGGNSLNNNPSLASKQRLRWTHELHERFVDAVAQLGGPDRATPKGVLRVMGVQGLTIYHVKSHLQKYRLAKYLPDSSSDGKKADKKESGDMLSSLDGPSGMQITEALKLQMEVQKRLHEQLEVQRQLQLRIEAQGKYLKKIIEEQQRLSGVITEVPGSGVSVPVSGDNCLESDNKTDPATPAPTSEGPLLDKAAKETAPAKSLSIDESFSSHHEPLTPDSGCHVNSSGESPKGERSVKKQRVGIGAAYAKQEMVLTHQILESSLSSSFHQPHSVFLNRDQFDPQAGISISNEDQLDQLEKVPSSNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZKD4</accession>
    <name>A0AA38ZKD4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Trichome birefringence-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_013030</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PC-esterase family. TBL subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZKD4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016413">
        <property type="term" value="F:O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029962">
        <property type="entry name" value="TBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026057">
        <property type="entry name" value="TBL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025846">
        <property type="entry name" value="TBL_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285:SF42">
        <property type="entry name" value="PROTEIN TRICHOME BIREFRINGENCE-LIKE 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285">
        <property type="entry name" value="PROTEIN TRICHOME BIREFRINGENCE-LIKE 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13839">
        <property type="entry name" value="PC-Esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14416">
        <property type="entry name" value="PMR5N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041276326" description="Trichome birefringence-like N-terminal domain-containing protein" evidence="7">
        <location>
            <begin position="28"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="Trichome birefringence-like N-terminal" evidence="9">
        <location>
            <begin position="45"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="Trichome birefringence-like C-terminal" evidence="8">
        <location>
            <begin position="98"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF14416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9690660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="367" mass="41843" checksum="EEF05B4105FF92F7" modified="2024-01-24" version="1">MGVSSVRCHLVVQGLLFLILLLQQARADHQVYNVSGLRSRKQVSSCNLFQGKWVFDASYPFYDASNCPFIDPEFDCQKYGRPDKQYLKYSWKPDSCDLPRFDGFDFLRRWRGKRIMFVGDSLSLNQWESLVCMIHQSASNAKTSFVKKDGLTTVTFDGYAVSVSLYRTPYLVDIVRESVGRVLKLDSIQAGDTWKQMDLLIFNSWHWWTHKGNSQGWDYMSEGTKLYKDMDRLTAFYKGLSTWASWVDLNIDPSKTKVFFQGISPTHYTGKEWNSQSKNCYGELEPLSGSTYPAGAPAAAAIVNQVLSKIRKPVYLLDITTLSQLRKDAHPSTYGEDHNGMDCSHWCLPGLPDTWNQLLYAALVMGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZLF4</accession>
    <name>A0AA38ZLF4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_013347</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZLF4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36617">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36617:SF15">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase zinc-binding" evidence="1">
        <location>
            <begin position="102"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9691127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="259" mass="30271" checksum="E29D610C1B7FC133" modified="2024-01-24" version="1">MLTRFVVGNGERIRFWEDLWWGDQPLGSQYPRLFRVVLDKNILISSILSSSRPFSWNFNFHHNLSDPEIEDLEGLMQSLDGLHLSPSVSDARFWPLSSSGLFSVKSFFLALSQFSGSPLVFPSKFVWKSQVPFKVKSFVWLGAHKKVNTNDMLQLRRPYKVLSPDICILCMKHRESIDHLFLHCSLTTGLWHRLFQLAKMNWVPPRIIFDMMSIKFNGFGVSKRGRVLWQAASIAIIWAMWWERNARIFEDKARNSEYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZLX6</accession>
    <name>A0AA38ZLX6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Germin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_013429</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May play a role in plant defense. Probably has no oxalate oxidase activity even if the active site is conserved.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Secreted</location>
            <location evidence="2 13">Extracellular space</location>
            <location evidence="2 13">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the germin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZLX6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02241">
        <property type="entry name" value="cupin_OxOx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000005">
        <property type="entry name" value="Germin-like protein subfamily 1 member 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006045">
        <property type="entry name" value="Cupin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001929">
        <property type="entry name" value="Germin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019780">
        <property type="entry name" value="Germin_Mn-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31238">
        <property type="entry name" value="GERMIN-LIKE PROTEIN SUBFAMILY 3 MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00190">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00325">
        <property type="entry name" value="GERMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00835">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00725">
        <property type="entry name" value="GERMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 13" id="KW-0052">Apoplast</keyword>
    <keyword evidence="7 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 10 13" id="KW-0464">Manganese</keyword>
    <keyword evidence="6 10 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0964">Secreted</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041489209" description="Germin-like protein" evidence="13">
        <location>
            <begin position="22"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="Cupin type-1" evidence="14">
        <location>
            <begin position="60"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="31"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU366015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9691250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="216" mass="22672" checksum="F983C231FA274827" modified="2024-01-24" version="1">MAIPLFVSGLLVLCFSIIAIASDPSPLQDFCVADANSTVLLNGLACKDPKLAQASDFSFSGLQIAGNTSNAVGSKVTPVTVAQLPGLNTLGISFARVDYAPWGINPPHTHPRASEILTVVKGCLEVGFVTSNPDNRLITKILQKGDVFVFPIGLIHFQRNVRPGSAVALAALSSQNPGVITIANAVFGSKPDISSDILAKAFQTDEAIVKDIQSKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZMN4</accession>
    <name>A0AA38ZMN4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ABC-type xenobiotic transporter</fullName>
            <ecNumber evidence="3">7.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_013352</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O + xenobioticSide 1 = ADP + phosphate + xenobioticSide 2.</text>
            <dbReference type="EC" id="7.6.2.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.2" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902417">
        <property type="term" value="F:(+)-abscisic acid D-glucopyranosyl ester transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008559">
        <property type="term" value="F:ABC-type xenobiotic transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18579">
        <property type="entry name" value="ABC_6TM_ABCC_D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18580">
        <property type="entry name" value="ABC_6TM_ABCC_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000013">
        <property type="entry name" value="ABC transporter C family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000024">
        <property type="entry name" value="ABC transporter C family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000450">
        <property type="entry name" value="ABC transporter C family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000163">
        <property type="entry name" value="Multidrug resistance-associated protein member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044746">
        <property type="entry name" value="ABCC_6TM_D1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044726">
        <property type="entry name" value="ABCC_6TM_D2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF375">
        <property type="entry name" value="ABC TRANSPORTER C FAMILY MEMBER 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="36"/>
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    <feature type="transmembrane region" description="Helical" evidence="14">
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            <begin position="111"/>
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    <feature type="transmembrane region" description="Helical" evidence="14">
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            <begin position="143"/>
            <end position="162"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="174"/>
            <end position="195"/>
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    <feature type="transmembrane region" description="Helical" evidence="14">
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            <begin position="338"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="428"/>
            <end position="456"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="528"/>
            <end position="548"/>
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    <feature type="transmembrane region" description="Helical" evidence="14">
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    <feature type="transmembrane region" description="Helical" evidence="14">
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            <end position="976"/>
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    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1046"/>
            <end position="1074"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1140"/>
            <end position="1160"/>
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        <location>
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            <end position="1194"/>
        </location>
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            <end position="583"/>
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        <source>
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        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50929"/>
        </source>
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        <source>
            <dbReference type="EMBL" id="KAJ9691138.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1624" mass="183001" checksum="75D584539D6D98FF" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZNZ5</accession>
    <name>A0AA38ZNZ5_VITRO</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_011404</name>
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    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
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            <dbReference type="EMBL" id="KAJ9692329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="230" mass="25446" checksum="E71A2A16C17D905F" modified="2024-01-24" version="1">MASSALHCNFALCSPIKKPLQTITTTSYTPIRCGPRDNRGPLMKGRVLSIEAIQAIQSLKRAHRGDPTKIDDLLSKTLSRLVKADLLATLNELLRQDQCDLALRVFSAVRSELWYKTELSLYADLVSALARKGMKEEIDRLICDLEGEGSVRCDDKGIVRLVKAVIAAERRESTVRIYGLMKRSGCGGGDEYVVRVLSRGLRRLGELGVADEVDLEFGRLSKGNLEKVEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
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    <name>A0AA38ZPI4_VITRO</name>
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        <recommendedName>
            <fullName evidence="6">AP2/ERF domain-containing protein</fullName>
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        <name evidence="7" type="ORF">PVL29_011805</name>
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    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
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            <taxon>Spermatophyta</taxon>
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            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
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    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
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                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692881.1"/>
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    <dbReference type="AlphaFoldDB" id="A0AA38ZPI4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ethylene-responsive transcription factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050913">
        <property type="entry name" value="AP2/ERF_ERF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31194:SF61">
        <property type="entry name" value="ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR ERF003"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31194">
        <property type="entry name" value="SHN SHINE , DNA BINDING / TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00367">
        <property type="entry name" value="ETHRSPELEMNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="6">
        <location>
            <begin position="7"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9692881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="177" mass="20053" checksum="77C56044620AB0FE" modified="2024-01-24" version="1">MAGQQQRYRGVRQRHWGSWVSEIRHPQLKTRIWLGTFETAEDAARAYDEAARLMCGPRARTNFTYNSDDSQSSSSKFLSATLTAKLQRCYMASPQGAIKPLPTDFQQPHYSPNFGTIDGMATRNCQVGVGSPAKRRRQVQGKWVGGEDEEFKVLEDDHIEQMIEELLHSGSIELSFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZQ99</accession>
    <name>A0AA38ZQ99_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_012162</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9693291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZQ99"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041206564" evidence="2">
        <location>
            <begin position="24"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9693291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="281" mass="29087" checksum="51C0D8D151C044CD" modified="2024-01-24" version="1">MARASSNFASGFFLLSIAALLHATPAFSDEFSSELCKGHGCHSKYPPPPPPPPPPPPPPPPIDPDCPPPPVDPPIVPPTPYHPHPTSPPPDPYGKVPPPPAPVPASPPDPNGKVPPQPAPPPYTDGKAPPPYPDGKAPPPYPDGKAPPPYNDGKAPPPYPDGKAPPPYTDGKAPPPYTGGKAPPPYPDGKAPPPYTDGKAPPPYTDGKAPPPYTDGKAPPPYPDGKAPPPYPYGNAPPPYTDGKAPPPYTDGKAPPPYPDGKAPPPPPCDELTYNSRKLKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZRA1</accession>
    <name>A0AA38ZRA1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Vacuolar cation/proton exchanger</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_012305</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Vacuolar cation/proton exchanger (CAX). Translocates Ca(2+) and other metal ions into vacuoles using the proton gradient formed by H(+)-ATPase and H(+)-pyrophosphatase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family. Cation/proton exchanger (CAX) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9693470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZRA1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009705">
        <property type="term" value="C:plant-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015369">
        <property type="term" value="F:calcium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.30:FF:000008">
        <property type="entry name" value="Vacuolar cation/proton exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.30:FF:000012">
        <property type="entry name" value="Vacuolar cation/proton exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="entry name" value="NCX, central ion-binding region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004713">
        <property type="entry name" value="CaH_exchang"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004798">
        <property type="entry name" value="CAX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00846">
        <property type="entry name" value="caca2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00378">
        <property type="entry name" value="cax"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31503">
        <property type="entry name" value="VACUOLAR CALCIUM ION TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31503:SF22">
        <property type="entry name" value="VACUOLAR CALCIUM ION TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12" id="KW-0050">Antiport</keyword>
    <keyword evidence="8 12" id="KW-0106">Calcium</keyword>
    <keyword evidence="6 12" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="10 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <keyword evidence="5 12" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="60"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="91"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="116"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="155"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="188"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="225"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="279"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="310"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="347"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="376"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="404"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="13">
        <location>
            <begin position="91"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="13">
        <location>
            <begin position="279"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU365028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9693470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="438" mass="48114" checksum="B414C7F9E609861C" modified="2024-01-24" version="1">MGSIEEKIDLESDEEIPFSTSQGTRKVHSFDFETSHIGSHAALSNSWIFKKMRTAPLRSVYIVLIKAKINMLLPFGPLAILLHYLTGKHGWVFFFSLLGITPLAERLGFATEQLAFYTGATVGGLLNATFGNATEMIISMYALKHGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIKNHTKVQEFNKAAALVNSGLLLMAVMGLMFPAVLHFTHTEVHFGKSELALSRFSSCIMLVAYASYLFFQLKSQPSLYSPIDEDTEKIEEDSDEDVPELTQREAIGWLAILTLWVSVLSGYLVDAIEGASESLNMPVAFISVILLPIVGNAAEHASAIMFAIKNKLDITIGVAIGSSTQISMFVIPFCVVVGWVMGQPMDLNFQLFETATLFIAVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFVHVDPTKDLYIVNDIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZRA6</accession>
    <name>A0AA38ZRA6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_009468</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9693529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZRA6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000258">
        <property type="entry name" value="Probable serine/threonine-protein kinase PBL8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000228">
        <property type="entry name" value="Serine/threonine-protein kinase BIK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050823">
        <property type="entry name" value="Plant_Ser_Thr_Prot_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45621">
        <property type="entry name" value="OS01G0588500 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0611">Plant defense</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="111"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="390"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="73"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="394"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9693529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="458" mass="50541" checksum="684C3EA79DE2EA17" modified="2024-01-24" version="1">MGNCGSTEDNPVVSHAQGPDLRPHFPLFFSHPLSPSLTLLLFAVQQLHILSSLSVKDAAPNCGKKHNRSVSDLSDHHSTPRNLDDSGKNSLLYTHVIDFTLFELETITKSFRSDYILGEGGFGTVYKGYIDENVRVGLKSLPVAVKVLNKEGFQGHREWLTEVNFLGQLRHPNLVKLIGYCCEDDHRLLVYEFMFRGSLENHLFRKATVPLSWATRMMIALGAAKGLAFLHNAERPVIYRDFKTSNILLDSDYTAKLSDFGLAKAGPQGDETHVSTRVMGTYGYAAPEYVMTGHLTARSDVYSFGVVLLELLTGRRSVDKTRPSKEQSLVDWARPKLNDKRKLLQIIDPRLENQYSVRAAQKACSLAYYCLSQNPKARPLMSDVVETLEPLQDSSGGTSEVSSSTKNLSVGSGGPFAKAGMSDYRMQHRFANKVGAGAVCRSPNPNCSPGGPAACRVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZRJ9</accession>
    <name>A0AA38ZRJ9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">pyruvate decarboxylase</fullName>
            <ecNumber evidence="6">4.1.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_009793</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2-oxocarboxylate + H(+) = an aldehyde + CO2</text>
            <dbReference type="Rhea" id="RHEA:11628"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="EC" id="4.1.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="12">Binds 1 Mg(2+) per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>a metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:25213"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the TPP enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.1" evidence="6"/>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9693993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZRJ9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004737">
        <property type="term" value="F:pyruvate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000949">
        <property type="term" value="P:aromatic amino acid family catabolic process to alcohol via Ehrlich pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036293">
        <property type="term" value="P:response to decreased oxygen levels"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02005">
        <property type="entry name" value="TPP_PDC_IPDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07038">
        <property type="entry name" value="TPP_PYR_PDC_IPDC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1220:FF:000009">
        <property type="entry name" value="Pyruvate decarboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000017">
        <property type="entry name" value="pyruvate decarboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000021">
        <property type="entry name" value="Pyruvate decarboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012110">
        <property type="entry name" value="PDC/IPDC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047214">
        <property type="entry name" value="TPP_PDC_IPDC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047213">
        <property type="entry name" value="TPP_PYR_PDC_IPDC-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43452">
        <property type="entry name" value="PYRUVATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43452:SF6">
        <property type="entry name" value="PYRUVATE DECARBOXYLASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036565">
        <property type="entry name" value="Pyruvt_ip_decrb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="11" id="KW-0456">Lyase</keyword>
    <keyword evidence="9 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 13" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme N-terminal TPP-binding" evidence="16">
        <location>
            <begin position="44"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme central" evidence="14">
        <location>
            <begin position="241"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="15">
        <location>
            <begin position="451"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="482"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="509"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="511"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR036565-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9693993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="605" mass="65049" checksum="8F004C6FB708C125" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZTQ0</accession>
    <name>A0AA38ZTQ0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic</fullName>
            <shortName evidence="9">RA</shortName>
            <shortName evidence="9">RuBisCO activase</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_010303</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 9">Activation of RuBisCO (ribulose-1,5-bisphosphate carboxylase/oxygenase; EC 4.1.1.39) involves the ATP-dependent carboxylation of the epsilon-amino group of lysine leading to a carbamate structure.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Plastid</location>
            <location evidence="1 9">Chloroplast stroma</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 9">Belongs to the RuBisCO activase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZTQ0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009570">
        <property type="term" value="C:chloroplast stroma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009579">
        <property type="term" value="C:thylakoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046863">
        <property type="term" value="F:ribulose-1,5-bisphosphate carboxylase/oxygenase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.1070:FF:000001">
        <property type="entry name" value="Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000258">
        <property type="entry name" value="Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.1070">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044960">
        <property type="entry name" value="RCA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048571">
        <property type="entry name" value="RuBisCO_activase_AAA_helical"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32429">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32429:SF44">
        <property type="entry name" value="RIBULOSE BISPHOSPHATE CARBOXYLASE_OXYGENASE ACTIVASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21228">
        <property type="entry name" value="RuBisCO_activase_AAA_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 9" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 9" id="KW-0934">Plastid</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="ATPase AAA-type core" evidence="11">
        <location>
            <begin position="171"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="Ribulose bisphosphate carboxylase/oxygenase activase AAA helical" evidence="12">
        <location>
            <begin position="317"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="457"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU369045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF21228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9694760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="482" mass="52948" checksum="50E7F932C069F0C8" modified="2024-01-24" version="1">MESAISFELCGLVANKKGTSWVQLNLNGSGTGASVPSSAFLGNSLKKVTSKIAHPRTSSGNFKVVAEVDEDKQTSKDKWKGLAFDTSDDQQDITRGKGMVDSLFQAPMESGTHYAVMSSYDYISTGLRQYNLDNTMDGFYIAPAFMDKLVVHITKNFMTLPNIKVPLILGIWGGKGQGKSFQCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIRKGKMCCLFINDLDAGAGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAPLIRDGRMEKFYWAPNREDRIGVCTGIFRSDDIPKEDIVKLVDTFPGQSIDFFGALRARVYDDEVRKWVVSVGVESIGKKLVNSKEGPPTFEKPKMTLEKLLEYGSMLVQEQENVKRVQLADKYLSEAALGDANEDAIKSGTFYGKAAQQVNLPVPEGCTDPSANNFDPTARSDDGSCLYQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZTV5</accession>
    <name>A0AA38ZTV5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transcription elongation factor spt6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_009988</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Transcription elongation factor that enhances transcription elongation by RNA polymerase II (RNAPII).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the SPT6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZTV5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008023">
        <property type="term" value="C:transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009793">
        <property type="term" value="P:embryo development ending in seed dormancy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034728">
        <property type="term" value="P:nucleosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140673">
        <property type="term" value="P:transcription elongation-coupled chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09928">
        <property type="entry name" value="SH2_Cterm_SPT6_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09918">
        <property type="entry name" value="SH2_Nterm_SPT6_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2740:FF:000002">
        <property type="entry name" value="Transcription elongation factor Spt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.850:FF:000001">
        <property type="entry name" value="Transcription elongation factor spt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3500.10:FF:000004">
        <property type="entry name" value="Transcription elongation factor spt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000256">
        <property type="entry name" value="Transcription elongation factor spt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.140:FF:000006">
        <property type="entry name" value="Transcription elongation factor spt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000047">
        <property type="entry name" value="Transcription elongation factor spt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000050">
        <property type="entry name" value="Transcription elongation factor spt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2740">
        <property type="entry name" value="Spt6, Death-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.850">
        <property type="entry name" value="Spt6, helix-hairpin-helix domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3500.10">
        <property type="entry name" value="Tex N-terminal region-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.140">
        <property type="entry name" value="YqgF/RNase H-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041692">
        <property type="entry name" value="HHH_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049540">
        <property type="entry name" value="Spt6-like_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042066">
        <property type="entry name" value="Spt6_death-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032706">
        <property type="entry name" value="Spt6_HHH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035420">
        <property type="entry name" value="Spt6_SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035018">
        <property type="entry name" value="Spt6_SH2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035019">
        <property type="entry name" value="Spt6_SH2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028231">
        <property type="entry name" value="Spt6_YqgF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023323">
        <property type="entry name" value="Tex-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017072">
        <property type="entry name" value="TF_Spt6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006641">
        <property type="entry name" value="YqgF/RNaseH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037027">
        <property type="entry name" value="YqgF/RNaseH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10145">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR SPT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10145:SF6">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR SPT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14635">
        <property type="entry name" value="HHH_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17674">
        <property type="entry name" value="HHH_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14633">
        <property type="entry name" value="SH2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21710">
        <property type="entry name" value="Spt6_S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14639">
        <property type="entry name" value="YqgF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036947">
        <property type="entry name" value="Spt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00732">
        <property type="entry name" value="YqgFc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="RuvA domain 2-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158832">
        <property type="entry name" value="Tex N-terminal region-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="S1 motif" evidence="8">
        <location>
            <begin position="603"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="683"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="932"/>
            <end position="1139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="692"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="937"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="976"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="1061"/>
            <end position="1110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR036947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9694273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1139" mass="126090" checksum="A3C83E01D5243E58" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZUP5</accession>
    <name>A0AA38ZUP5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Gamma-tubulin complex component</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_010847</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TUBGCP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZUP5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000930">
        <property type="term" value="C:gamma-tubulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043015">
        <property type="term" value="F:gamma-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051011">
        <property type="term" value="F:microtubule minus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051225">
        <property type="term" value="P:spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1900">
        <property type="entry name" value="Gamma-tubulin complex, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007259">
        <property type="entry name" value="GCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040457">
        <property type="entry name" value="GCP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042241">
        <property type="entry name" value="GCP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041470">
        <property type="entry name" value="GCP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302">
        <property type="entry name" value="GAMMA TUBULIN COMPLEX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302:SF14">
        <property type="entry name" value="GAMMA-TUBULIN COMPLEX COMPONENT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04130">
        <property type="entry name" value="GCP_C_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17681">
        <property type="entry name" value="GCP_N_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gamma tubulin complex component protein N-terminal" evidence="7">
        <location>
            <begin position="195"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="Gamma tubulin complex component C-terminal" evidence="6">
        <location>
            <begin position="504"/>
            <end position="847"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9695566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="854" mass="96968" checksum="DA74165BB99C527B" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZXL8</accession>
    <name>A0AA38ZXL8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_008418</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZXL8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22152">
        <property type="entry name" value="F-box_AtAFR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46344:SF21">
        <property type="entry name" value="F-BOX_KELCH-REPEAT PROTEIN SKIP30 ISOFORM X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46344">
        <property type="entry name" value="OS02G0202900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="F-box" evidence="3">
        <location>
            <begin position="8"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9696164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="345" mass="38435" checksum="AC9F462B7AEA57DD" modified="2024-01-24" version="1">MSGLIEGLPDAVALRCLAWVPFYLHPRLELVSRSWRDAIRGPELFKARQEVGSSEDLLCVCAFDPENLWQLYDPRKDLWISLPVLPSRIRHLAHFGAVSTAGKLFVLGGGSDAVDPLTGDQDGSFATNEVWSYDPIIRQWAPRAPMLVPRAMFACCVLDGKIVVAGGFTSCRKSISQAEIYDPEKDAWVSIPDLHRTHNSACSGVVIDGKVHVLHKGLTTVQILDRVGPGWRVEDYGWLQGPMAIVQGALYVVSHGIIFKQEREVRKMVVSASEFRRRIGFAMTGLRDEIYVIGGVIGPDRWNWDIKPLSDVDVLTIGSERPAWRQVSPMSRCRGTIFGCTQLRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZXZ6</accession>
    <name>A0AA38ZXZ6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Alpha/beta hydrolase fold-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_008525</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 'GDXG' lipolytic enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZXZ6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010331">
        <property type="term" value="F:gibberellin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048444">
        <property type="term" value="P:floral organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048530">
        <property type="term" value="P:fruit morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009740">
        <property type="term" value="P:gibberellic acid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009939">
        <property type="term" value="P:positive regulation of gibberellic acid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010325">
        <property type="term" value="P:raffinose family oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000087">
        <property type="entry name" value="Gibberellin receptor GID1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050466">
        <property type="entry name" value="Carboxylest/Gibb_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002168">
        <property type="entry name" value="Lipase_GDXG_HIS_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033140">
        <property type="entry name" value="Lipase_GDXG_put_SER_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23024">
        <property type="entry name" value="ARYLACETAMIDE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23024:SF98">
        <property type="entry name" value="GIBBERELLIN RECEPTOR GID1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01173">
        <property type="entry name" value="LIPASE_GDXG_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01174">
        <property type="entry name" value="LIPASE_GDXG_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0939">Gibberellin signaling pathway</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="8">
        <location>
            <begin position="108"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9696344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="344" mass="38891" checksum="2F03818E78EFDC8D" modified="2024-01-24" version="1">MAGSDEVNHNESKSVVPLNTWILISNFKLAYNLLRRPDGTFERELAEFLERKAPANSFPVDGVFSFDIVDKTTGLLNRVYQPAPENEAQWGIIELEKPLSTTEIVPVILFFHGGSFTHSSANSAIYDYFCRRLVGNCKAVVVSVNYRRSPEHRYPCAYDDGWAALKWVKSRSWLQSGKDSKVHVYLAGDSSGGNITHHVAVRAAESGIEVLGNILLHPMFGGQERTESEKRLDGKYFVTIQDRDWYWRAFLPEGEDRDHPACNPFGPRGKSLEGLNFPKSLVVVAGFDLVQDWQLAYVEGLKKAGQDVNLLFLEQATIGFYFLPNNDHFYCLMEEIKNFVNPNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A0J2</accession>
    <name>A0AA39A0J2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA-directed RNA polymerase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_007548</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">DNA-dependent RNA polymerase which catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A0J2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060213">
        <property type="term" value="P:positive regulation of nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045948">
        <property type="term" value="P:positive regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04329">
        <property type="entry name" value="RNAP_II_Rpb7_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04462">
        <property type="entry name" value="S1_RNAPII_Rpb7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000043">
        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.120:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.120">
        <property type="entry name" value="RNA polymerase Rpb7-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036898">
        <property type="entry name" value="RNA_pol_Rpb7-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045113">
        <property type="entry name" value="Rpb7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005576">
        <property type="entry name" value="Rpb7-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12709:SF4">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12709">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II, III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03876">
        <property type="entry name" value="SHS2_Rpb7-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88798">
        <property type="entry name" value="N-terminal, heterodimerisation domain of RBP7 (RpoE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="RNA polymerase Rpb7-like N-terminal" evidence="8">
        <location>
            <begin position="8"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="7">
        <location>
            <begin position="79"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU369086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9698524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="176" mass="19590" checksum="9A062CDDA27FEF6A" modified="2024-01-24" version="1">MFFHIVLERNMQLHPRHFGRHLRDNLVAKLVKDVEGTCSGRHGFVVAITGIENVGKGLIRDGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQSGDMPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEIFCIGTIKDDFLGVINDPVTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A349</accession>
    <name>A0AA39A349_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_008239</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9699552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A349"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000009">
        <property type="entry name" value="transcription factor MYB1R1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000154">
        <property type="entry name" value="Transcription factor SRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006447">
        <property type="entry name" value="Myb_dom_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01557">
        <property type="entry name" value="myb_SHAQKYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44042">
        <property type="entry name" value="DUPLICATED HOMEODOMAIN-LIKE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44042:SF67">
        <property type="entry name" value="MYB-LIKE PROTEIN I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Myb-like" evidence="7">
        <location>
            <begin position="16"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="115"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="7">
        <location>
            <begin position="115"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="8">
        <location>
            <begin position="123"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="92"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="14"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="111"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9699552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="225" mass="25521" checksum="BE2C0A2923991272" modified="2024-01-24" version="1">MFPNDSWMMMVNQSPSPSSSSSSWSRHQDKLFERALVVVPEETPDRWDKIAAQVPGKSSSEVRRHYDDLVHDVAEIDSGRVELPLYEDESCGSPWASDSRAGQVSFSPRPRQSESERKKGVPWTEEEHRLFLIGLQRYGKGDWRSISRNAVVSRTPTQVASHAQKYFMRLTSGKKDKKRSSIHDITTVDTTNSLPHTNTQTWVGESLASQIPYYEEGPPSFGFSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A3J3</accession>
    <name>A0AA39A3J3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein high chlorophyll fluorescent 107</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_005590</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9699792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A3J3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001624">
        <property type="entry name" value="PsbB mRNA maturation factor Mbb1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:002248">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044624">
        <property type="entry name" value="Mbb1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44917">
        <property type="entry name" value="PROTEIN HIGH CHLOROPHYLL FLUORESCENT 107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44917:SF1">
        <property type="entry name" value="PROTEIN HIGH CHLOROPHYLL FLUORESCENT 107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="282"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="54"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="555"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="92"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="568"/>
            <end position="585"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9699792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="649" mass="73298" checksum="DA1EBE98430A5333" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A490</accession>
    <name>A0AA39A490_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_006145</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the major facilitator superfamily. Phosphate:H(+) symporter (TC 2.A.1.9) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A490"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17364">
        <property type="entry name" value="MFS_PhT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000175">
        <property type="entry name" value="Inorganic phosphate transporter 1-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004738">
        <property type="entry name" value="Phos_permease"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00887">
        <property type="entry name" value="2A0109"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0769">Symport</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="20"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="163"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="213"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="349"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="373"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="404"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="443"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="484"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="11">
        <location>
            <begin position="24"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="510"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="526"/>
            <end position="538"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9700683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="538" mass="58980" checksum="B028E52759277712" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A5Y3</accession>
    <name>A0AA39A5Y3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Calcium-transporting ATPase</fullName>
            <ecNumber evidence="19">7.2.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PVL29_006009</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">Catalyzes the hydrolysis of ATP coupled with the transport of calcium.</text>
    </comment>
    <comment type="function">
        <text evidence="18">This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol out of the cell or into organelles.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17 19">
            <text>Ca(2+)(in) + ATP + H2O = Ca(2+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18105"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 19">Membrane</location>
            <topology evidence="1 19">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 19">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.10" evidence="19"/>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A5Y3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005388">
        <property type="term" value="F:P-type calcium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd02081">
        <property type="entry name" value="P-type_ATPase_Ca_PMCA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000039">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000006">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000013">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000018">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006408">
        <property type="entry name" value="P-type_ATPase_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01517">
        <property type="entry name" value="ATPase-IIB_Ca"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093:SF434">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE 13, PLASMA MEMBRANE-TYPE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093">
        <property type="entry name" value="CATION TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 19" id="KW-0106">Calcium</keyword>
    <keyword evidence="4 19" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="11" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="15 19" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="16 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 19" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="140"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="335"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="385"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="808"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="832"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="945"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="975"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="20">
        <location>
            <begin position="94"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00053300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU361146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAJ9700484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1011" mass="111292" checksum="00748E0A019E26B8" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A8Z0</accession>
    <name>A0AA39A8Z0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein GPR107</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_004732</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A8Z0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054103">
        <property type="entry name" value="CAND6-7_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053937">
        <property type="entry name" value="GOST_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009637">
        <property type="entry name" value="GPR107/GPR108-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21229">
        <property type="entry name" value="LUNG SEVEN TRANSMEMBRANE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21229:SF21">
        <property type="entry name" value="OS04G0508600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21904">
        <property type="entry name" value="CAND6-7_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06814">
        <property type="entry name" value="GOST_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="15"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="181"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="210"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="246"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="284"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="315"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="363"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="CAND6/7 N-terminal" evidence="8">
        <location>
            <begin position="36"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="GOST seven transmembrane" evidence="7">
        <location>
            <begin position="177"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF06814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF21904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9703081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="444" mass="51292" checksum="B52EDDCBAE06285E" modified="2024-01-24" version="1">MGNFNFHLNLPHRPILHLLNVLIIISLIPYSVSEIKNTRIIDDSRPMILFERFGIAQYGNVAVSVRDFSWKSTRQNAELEPSSMGFFLVRDVSFPSIFNESEYADNFCILWSRFVKIIFKFDNLAAYNGSMLIEEPDEYSLIFGNCQSETQVSMDVHTEMYNMLDGNKNFLPAGQTPLPRLYFIFFLIYSIFFSVWIFICIKQRPDIHKIHMIMGALLLFKALKMICASEDKMYVRKTGTPHGWDVAFYVFGFFKGIMLFTVIVLIGTGWSFLKPYLQEREKKVLMVVIPLQVMENIASMIIAETGPATKDWLTWNQIFLLLDVACCCAVFLPIVWSIRGLREASKTDGKAARNLEKLTLFKHFYIVVVGYLYFTRIVVPAVSSVISYRFEWVMTAADEGASLAFYLFIFHNFQPTQKNPYLVIDDEDEAEAAHMLEHDDSFEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AC59</accession>
    <name>A0AA39AC59_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Chalcone synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_002753</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>4-coumaroyl-CoA + 3 malonyl-CoA + 3 H(+) = trans-resveratrol + 4 CO2 + 4 CoA</text>
            <dbReference type="Rhea" id="RHEA:11936"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:45713"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57355"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="EC" id="2.3.1.95"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the thiolase-like superfamily. Chalcone/stilbene synthases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AC59"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050350">
        <property type="term" value="F:trihydroxystilbene synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030639">
        <property type="term" value="P:polyketide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00831">
        <property type="entry name" value="CHS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000014">
        <property type="entry name" value="Chalcone synthase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000025">
        <property type="entry name" value="Chalcone synthase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012328">
        <property type="entry name" value="Chalcone/stilbene_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001099">
        <property type="entry name" value="Chalcone/stilbene_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011141">
        <property type="entry name" value="Polyketide_synthase_type-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11877">
        <property type="entry name" value="HYDROXYMETHYLGLUTARYL-COA SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11877:SF10">
        <property type="entry name" value="TYPE III POLYKETIDE SYNTHASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02797">
        <property type="entry name" value="Chal_sti_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00195">
        <property type="entry name" value="Chal_sti_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Chalcone/stilbene synthase N-terminal" evidence="4">
        <location>
            <begin position="1"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="Chalcone/stilbene synthase C-terminal" evidence="5">
        <location>
            <begin position="167"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00048068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9704337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="319" mass="35081" checksum="158FF2743579C905" modified="2024-01-24" version="1">MSEEILRKYPELVIEGQPTVKQRLDICNKAVTQMAIDASKACIKNWGRSVSEITHLVYVSSSEARLPGGDLYLARGLGLSPETHRVQLYFMGCSGGVAGLRVAKDIAENNPESRVLLATSETTIIGFKPPSADRPYDLVGVALFGDGAGAMIIGSDPIPSTERPLFELHTAIQNFLPDTEKTIDGRLTEEGISFKLARELPQIIEDHIEAFCDKLIRNVGFSDKDYNKIFWAVHPGGPAILNRMEKRLDLLPEKLNASRRALADYGNASSNTIVYVLEYMLEESSKTKRQDQGDGDWGLILAFGPGITFEGILARNLTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AEI9</accession>
    <name>A0AA39AEI9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_001583</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AEI9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000030">
        <property type="entry name" value="F-box/kelch-repeat protein At3g61590"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000076">
        <property type="entry name" value="F-box/kelch-repeat protein At3g61590 family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056592">
        <property type="entry name" value="Beta-prop_At3g26010-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045048">
        <property type="entry name" value="FBXO31/39"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011043">
        <property type="entry name" value="Gal_Oxase/kelch_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011498">
        <property type="entry name" value="Kelch_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10706">
        <property type="entry name" value="F-BOX FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10706:SF130">
        <property type="entry name" value="F-BOX ONLY PROTEIN 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24750">
        <property type="entry name" value="b-prop_At3g26010-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07646">
        <property type="entry name" value="Kelch_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50965">
        <property type="entry name" value="Galactose oxidase, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="F-box" evidence="3">
        <location>
            <begin position="48"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9706082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="406" mass="46019" checksum="9A5076876D844885" modified="2024-01-24" version="1">MEGETSWIDYQFDDNRTQVLDLRVFDSYPELSDDDKSDSNLISIDSILPDDLLERILALLPIASIFKAAAVSRKWCEIVHSRRFLCNASRVLVPKPWYFMFTGSDEPTGYAYDPMDRKWYSFQLPHIETSSWFIASSYGLVCFMDNDSRSELYVCNLITKDHRKLVEPPGQKFSDYSALAISVDSPSHSYTVAVVKSKQIPNNFVQWNLSIHCYSSETGMWVSPVMELMTGWRGGDDSVICDGVLYFLVHSTTLTGNQHALATFNLSSRPSNGLSIDSFIPVPCSLTCGRLMNMNGKLVMVGGIGKHDRPGIIKGIGIWALKGRDWEEVSRMPHRFFQGFGEFDDVFASSGFNSLVYIQSYGAPALLVFDINLRQWKWSQKCPVTKRFPLQLFTGFCFEPRLEIAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AER4</accession>
    <name>A0AA39AER4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_003670</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AER4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000111">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046956">
        <property type="entry name" value="RLP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063">
        <property type="entry name" value="LRR RECEPTOR-LIKE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063:SF98">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE FLS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041204816" description="Leucine-rich repeat-containing N-terminal plant-type domain-containing protein" evidence="13">
        <location>
            <begin position="22"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="851"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="14">
        <location>
            <begin position="34"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="15">
        <location>
            <begin position="106"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="15">
        <location>
            <begin position="301"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9705702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="913" mass="102129" checksum="05AE28A1F5148612" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AG24</accession>
    <name>A0AA39AG24_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_001780</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AG24"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33472:SF28">
        <property type="entry name" value="BROMO AND FHA DOMAIN-CONTAINING PROTEIN DDB_G0267958"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33472">
        <property type="entry name" value="OS01G0106600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="159"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="175"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9706437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="190" mass="20940" checksum="0EF52D71374D57D7" modified="2024-01-24" version="1">MRCRSTPNFICPALQQNQPTHPSVFPTSPPLKTMLPEQMKPGEGEKQKQFDREIRDMISALTTRLSNLQQVQKQGSSQQDDEDDRGVRIITLAGSNTGATMRGELDEKPGPPGLSLGENEALSTYVNSNFQSVNNSIMMGGSYSTNDPGVHMDITDLVEHHGHKPVKHGKKGKKKDLVTAQDDHHSDHSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AGM2</accession>
    <name>A0AA39AGM2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CAAX prenyl protease 2/Lysostaphin resistance protein A-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_001569</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AGM2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004175">
        <property type="term" value="F:endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080120">
        <property type="term" value="P:CAAX-box protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003675">
        <property type="entry name" value="Rce1/LyrA-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43592">
        <property type="entry name" value="CAAX AMINO TERMINAL PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43592:SF24">
        <property type="entry name" value="CAAX AMINO TERMINAL PROTEASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02517">
        <property type="entry name" value="Rce1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="253"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="284"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="308"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="CAAX prenyl protease 2/Lysostaphin resistance protein A-like" evidence="2">
        <location>
            <begin position="255"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9706063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="347" mass="39049" checksum="A5B4408F0BAE2FBF" modified="2024-01-24" version="1">MWSSCSHALSRCPSLPPTISRLRLPNRPGTLYPLSSGFRISESHSKFPKSKWKISCFRHEDSSSETPKSESIEDILSGELVKPEFNKPSTAQKDWASSFREAADVILRVIGKPWVVPWTSETILQVMLLWIVSFWFVGSWMIPFTAHVVGFSKESLTYRGQALYSLLTDVAEGLAGIAILHRCLSRFYPLPSDWFKFSLRGNWLADVAIGCLMFPLVNRLSQFNLDLLPLLPSTPVTLSSVEQSIMARDPVAMGLYALVVSVCAPVWEEIVFRGFLLPSLTKYMPVWCSILVSSVAFALAHFNVQRMLPLIFLGVVMGVIFARSRNLLPSMLLHSLWNGFVFLDLMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AHA9</accession>
    <name>A0AA39AHA9_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_002559</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AHA9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036377">
        <property type="term" value="P:arbuscular mycorrhizal association"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040036">
        <property type="entry name" value="CYCLOPS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36890">
        <property type="entry name" value="PROTEIN CYCLOPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36890:SF1">
        <property type="entry name" value="PROTEIN CYCLOPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9707582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="161" mass="17684" checksum="19541FDEA11A7C13" modified="2024-01-24" version="1">MEAMNDASGHSVNKMKQEFTEPNSFSHAKGNARCKELGNQIEKEAAEDSSIGYYGASEVVLFNTVGQGQGNNIHESSGTFQEESPVQVMDHGYLQINVGSVDLNFRGFRYSSESNPIELSFSITFTIEHSFPITFTKQIFCCCIINLSQSSCYGPSNSGQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AIG9</accession>
    <name>A0AA39AIG9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_000345</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 15 16">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PP2C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AIG9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000013">
        <property type="entry name" value="probable protein phosphatase 2C 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="12">
        <location>
            <begin position="27"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9708240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="427" mass="46017" checksum="5435B35EE5ACB933" modified="2024-01-24" version="1">MASGEGRRHRNLVPLAALINREMRSEKMEKPTVRYGCAAQSRKGEDYFLMKTDCQRIPGNSSSTFSVFAILDGHNGNAAAIFTRENLLNHVLGAMPRGLGREEWLQALPRALVAGFVKTDKEFQSRGETSGTTATFVIVDGWTVTVASVGDSRCILDTQGGAVSTLTVDHRLEENVEERERVTASGGEVGRLSIVGGAEIGPLRCWPGGLCLSRSIGDMDVGEFIVPIPYVKQVKLSNAGGRLIIASDGIWDALCSEMAAKSCRGLPAELAARQVVKEALRTRGLKDDTTCIVVDIIPPDNSIQPSTPPKKQNKLRALLFRKRSRDAVNKLSKKLSAIGIVEELFEEGSAMLAERLGSEECPGQPTSGLFMCAVCQVDLAPSEGISVHAGSIFSTSSKPWQGPFLCADCRNKKDAMEGKRPSGVKVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AJ27</accession>
    <name>A0AA39AJ27_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_002244</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AJ27"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049198">
        <property type="entry name" value="DUF6865"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35282:SF11">
        <property type="entry name" value="EG5651"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35282">
        <property type="entry name" value="F5D14.24 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21737">
        <property type="entry name" value="DUF6865"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9707159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="78" mass="8421" checksum="A338D20FE944C422" modified="2024-01-24" version="1">MDNRSPGQEGSLELARELLIAISYCTPEKVLSLNCTPKDFNDANGVALTDGDGAEFFRSELISISYAQSPDSKVLPVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AJR9</accession>
    <name>A0AA39AJR9_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_000764</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AJR9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012442">
        <property type="entry name" value="DUF1645_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33095:SF23">
        <property type="entry name" value="DUF1645 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33095">
        <property type="entry name" value="OS07G0619500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07816">
        <property type="entry name" value="DUF1645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="264"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9708930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="337" mass="37276" checksum="E1E9590B8D2709F2" modified="2024-01-24" version="1">MQESMALNLSPSYNRHSSEDFAETAGNITQAFGAMDVGSFDFSQNLQVIVSEDNNGEALLEENSKDHGLVEISQPHDEAEEEGKEEEEEEEEEEEDNSDVEFSFVRADPDASPISADDIFFNGQIRPMFPLFNRDLLFADSHEGDSEASGKASPLRAPLRKLFVEKSEHPSSCSSSESDELEGVPPGTYCAWSEKAEEASPGACKKSNSTGFSKIWRFRDLVLRSNSDGKDAFVFLDPSAAKAKTVQTDEKVDRAKKIEKTEVSVEKRNSGDGKVAGSKVKGKGVKGEKRVSAHESLYIKNRALREGGRRKSYLPYRQDLVGFFTNVNGLSRNVHPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AK60</accession>
    <name>A0AA39AK60_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_000901</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AK60"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032872">
        <property type="entry name" value="WAK_assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025287">
        <property type="entry name" value="WAK_GUB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33355:SF10">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33355">
        <property type="entry name" value="WALL-ASSOCIATED RECEPTOR KINASE CARBOXY-TERMINAL PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13947">
        <property type="entry name" value="GUB_WAK_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14380">
        <property type="entry name" value="WAK_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041359453" description="non-specific serine/threonine protein kinase" evidence="8">
        <location>
            <begin position="25"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="281"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="Wall-associated receptor kinase galacturonan-binding" evidence="9">
        <location>
            <begin position="27"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="Wall-associated receptor kinase C-terminal" evidence="10">
        <location>
            <begin position="207"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF14380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9709158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="301" mass="33088" checksum="B30499CCA56D02FA" modified="2024-01-24" version="1">MKPKCLSITFLCISLILLPTLISSQSCQRTCGNQHLKYPFGSGPGCGDPRFQPYVTCSQEKLTLTTHTGCYTITNIDYNSQVLYITDPSMSTCSYAQPSKGFGLDWNAPFSFHDGNVFALLDCSITSSPIYKPDGLYGGGNSTLVPLCDREGASICSLLYSCRPISMLNTPISTCCVYTPVDLGPSFEMDLQKLKCTSYSGFYSYNGQDSNPQTWKYGVALKYKFSINNDYPGACADCERSNGVCGYTGDYNSFICNCPGGVNTTGNCFFSAYWNNGLTPLPWQTGVWLIYYLALILVWIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AM26</accession>
    <name>A0AA39AM26_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ribosomal protein/NADH dehydrogenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_001509</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complex I NDUFA2 subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9710071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AM26"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000179">
        <property type="entry name" value="NADH-ubiquinone oxidoreductase 10.5 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016464">
        <property type="entry name" value="NADH_Ub_cplx-1_asu_su-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007741">
        <property type="entry name" value="Ribosomal_mL43/mS25/NADH_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12878:SF0">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12878">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE B8 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05047">
        <property type="entry name" value="L51_S25_CI-B8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005822">
        <property type="entry name" value="NDUA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00916">
        <property type="entry name" value="L51_S25_CI-B8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Ribosomal protein/NADH dehydrogenase" evidence="11">
        <location>
            <begin position="20"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="10">
        <location>
            <begin position="19"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR005822-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9710071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="98" mass="11131" checksum="DCC8A8F150C23648" modified="2024-01-24" version="1">MAWRGQLSRNLKELRILFCQTSPASSSARAFVERNYKDLKTLNPKFPILIRECNGTRPQLWARYDMGVERGIHLEGLTEEQISKAVEDLVKLGAALKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39APD4</accession>
    <name>A0AA39APD4_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_001074</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39APD4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36617">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36617:SF15">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9709424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="122" mass="14159" checksum="E6E7C9AAC93257DB" modified="2024-01-24" version="1">MWKAINKVGQLVTPYFGFEVGDGKNVRFWKDKWCGTSPLSEAFPSLFALATSKEVWVNEVWTAGGDRRGSWTPTFNKPFNDWEMEEVGRLLCYLEGKKLREEEEDRVRWENSKDGVFSVKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D4M7</accession>
    <name>A0AA39D4M7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Cellulose synthase</fullName>
            <ecNumber evidence="23">2.4.1.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="ORF">PVL29_027095</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="26">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18 23">
            <text>[(1-&gt;4)-beta-D-glucosyl](n) + UDP-alpha-D-glucose = [(1-&gt;4)-beta-D-glucosyl](n+1) + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19929"/>
            <dbReference type="Rhea" id="RHEA-COMP:10033"/>
            <dbReference type="Rhea" id="RHEA-COMP:10034"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18246"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.4.1.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="23">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="23">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 23">Glycan metabolism; plant cellulose biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 23">Cell membrane</location>
            <topology evidence="2 23">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 23">Belongs to the glycosyltransferase 2 family. Plant cellulose synthase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="26">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.12" evidence="23"/>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D4M7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016760">
        <property type="term" value="F:cellulose synthase (UDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030244">
        <property type="term" value="P:cellulose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16617">
        <property type="entry name" value="mRING-HC-C4C4_CesA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000031">
        <property type="entry name" value="Cellulose synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000009">
        <property type="entry name" value="Cellulose synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005150">
        <property type="entry name" value="Cellulose_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027934">
        <property type="entry name" value="CES_Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13301">
        <property type="entry name" value="X-BOX TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03552">
        <property type="entry name" value="Cellulose_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14569">
        <property type="entry name" value="zf-UDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5 23" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17 23" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="12 23" id="KW-0135">Cellulose biosynthesis</keyword>
    <keyword evidence="6 23" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="16" id="KW-0464">Manganese</keyword>
    <keyword evidence="15 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 23" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 23" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 23" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11 23" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 22 23" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="290"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="317"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="876"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="908"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="946"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="989"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="1026"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="1058"/>
            <end position="1078"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="25">
        <location>
            <begin position="39"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="246"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="24">
        <location>
            <begin position="253"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="active site" evidence="19">
        <location>
            <position position="411"/>
        </location>
    </feature>
    <feature type="active site" evidence="19">
        <location>
            <position position="798"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="375"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="381"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="382"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="411"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="552"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="553"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="577"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR605150-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR605150-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR605150-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="RuleBase" id="RU361116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EMBL" id="KAJ9670946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1097" mass="124002" checksum="AE054C933E04465C" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D5K4</accession>
    <name>A0AA39D5K4_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_025546</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D5K4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010333">
        <property type="term" value="F:terpene synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016102">
        <property type="term" value="P:diterpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00684">
        <property type="entry name" value="Terpene_cyclase_plant_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.600.10:FF:000007">
        <property type="entry name" value="Isoprene synthase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.130:FF:000001">
        <property type="entry name" value="Isoprene synthase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.130">
        <property type="entry name" value="Terpene synthase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034741">
        <property type="entry name" value="Terpene_cyclase-like_1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044814">
        <property type="entry name" value="Terpene_cyclase_plant_C1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001906">
        <property type="entry name" value="Terpene_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036965">
        <property type="entry name" value="Terpene_synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050148">
        <property type="entry name" value="Terpene_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005630">
        <property type="entry name" value="Terpene_synthase_metal-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225:SF221">
        <property type="entry name" value="(-)-GERMACRENE D SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225">
        <property type="entry name" value="OS04G0344100 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01397">
        <property type="entry name" value="Terpene_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03936">
        <property type="entry name" value="Terpene_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01019">
        <property type="entry name" value="Terpene_Cyclase_Like_1_C_Termi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01604">
        <property type="entry name" value="Terpene_Cyclase_Like_1_C_Termi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Terpene synthase N-terminal" evidence="6">
        <location>
            <begin position="32"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="Terpene synthase metal-binding" evidence="7">
        <location>
            <begin position="262"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="47"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9671951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="557" mass="64180" checksum="592E0516823761F3" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D7G4</accession>
    <name>A0AA39D7G4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">PGG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_025598</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D7G4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000473">
        <property type="entry name" value="Ankyrin repeat family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026961">
        <property type="entry name" value="PGG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24186:SF46">
        <property type="entry name" value="PROTEIN ACCELERATED CELL DEATH 6-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24186">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13962">
        <property type="entry name" value="PGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="438"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="479"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="515"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="549"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="68"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="113"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="211"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="315"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="PGG" evidence="10">
        <location>
            <begin position="432"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="366"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="366"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9672022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="600" mass="66551" checksum="496CFB1965189635" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D7T6</accession>
    <name>A0AA39D7T6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_023416</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D7T6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080043">
        <property type="term" value="F:quercetin 3-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080044">
        <property type="term" value="F:quercetin 7-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000027">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000055">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058980">
        <property type="entry name" value="Glyco_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926">
        <property type="entry name" value="GLUCOSYL/GLUCURONOSYL TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926:SF774">
        <property type="entry name" value="UDP-GLYCOSYLTRANSFERASE 85A1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26168">
        <property type="entry name" value="Glyco_transf_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase N-terminal" evidence="5">
        <location>
            <begin position="23"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF26168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9673854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="495" mass="55097" checksum="5F00EA9AFEFD5831" modified="2024-01-24" version="1">MREGEEEGESSMAIVEKPRPHAVCVPFPAQGHINPMLKLAKLLHHKGFHITFVNTEFNHQRLLKSRGPNSLRGLPSFQFETIPDGLPPSDIDATQDVPSLCASTHNNCLAPFRDLLAKLNNTSSSKVPPVTCIVSDGIMSFTLKAAEELGIPEVFFWTTSACGFMGYVQYRHLIDRGFFPLKDESCLTNGHLDTVVDWIPAMKGVRLRDLPSFIRTTNPDDIVVNFAMGEVERTNDASAILLNTFDELEHEVLQALSTMFPPIYTIGPLQLLLNQMPDNDIKSIESNLWKEEPGCLEWLDAKEPESVVYVNFGSVTVMTPQQLIEFAWGLANTNLKFLWIIRPDLVAGDAAILPADFVAQTKERSLLASWCPQERVLTHPAIGGFLTHCGWNSTIESLCGGVPMICWPFFAEQLTNCRYCCTEWGVGMEIGNDVTRDEVESLVRGLMEGEKGKEMKKKAMEWKRMAEAATTTPAGSSYSNLDKMINQVLLSKSPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D9J4</accession>
    <name>A0AA39D9J4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PB1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_024235</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D9J4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053198">
        <property type="entry name" value="Gynoecium_Dev_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000270">
        <property type="entry name" value="PB1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31066:SF85">
        <property type="entry name" value="OS02G0809100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31066">
        <property type="entry name" value="OS05G0427100 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00564">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00666">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PB1" evidence="1">
        <location>
            <begin position="3"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9675205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="100" mass="11418" checksum="BD52CF0B9A909E6F" modified="2024-01-24" version="1">MRLPSSKLGYVGGNTHIISIDRGIGFLKLRSKISKLCPDIRSFFLKYRLPESDPIHGDTTNLVLITSDDDVRCMVDEYDKMDFYGQQTRLRIFVCSDNGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DA46</accession>
    <name>A0AA39DA46_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_025023</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9676299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DA46"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053211">
        <property type="entry name" value="DNA_repair-toleration"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48060">
        <property type="entry name" value="DNA DAMAGE-REPAIR/TOLERATION PROTEIN DRT100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48060:SF21">
        <property type="entry name" value="L DOMAIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041450930" description="Leucine-rich repeat-containing N-terminal plant-type domain-containing protein" evidence="8">
        <location>
            <begin position="27"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="9">
        <location>
            <begin position="31"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9676299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="479" mass="52559" checksum="474E867FA09EE0A0" modified="2024-01-24" version="1">MLALNSSLFFLLLSLLTLQCLTAASAACHVDDESGLLAFKSAITHDPSGMLQNWKSGTDCCKWPGITCRAGNRVTSLSLTGQPGKQNSFLSGTISPSLVKVQNLDGIYLQNLRKITGPFPALLFRLPKLLFVYIENNQLSGPLPRDIGNLTQLNALSFEGNRFSGPIPSSISQLTGLSQLKLGDNLLTGTVPAGVSRLKDLTFLSLERNGLSGSIPDFFSSFSNLRILQLSHNKFSGKIPNSISSLSPKLAYLELGHNSLVGQILDFLGNFTALDTLDLSWNQFSGTVPKTFAKLTKIFNLDLSHNFLVDPFPEMSVKGIESLDLSYNHFHLGTIPKWVTSSEIIYSLKLAKCGLKFKLDDWKPSETYFYDYIDLSENEITGSPIQLLNKTDYLVGFWASGNQLRFDLSKLRIVKTLKYLDVSRNMVFGKVPEGITGLEKLNVSHNHLCGRLPPSKFPASAFQGNDCLCGSPLSACKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DCH9</accession>
    <name>A0AA39DCH9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_020805</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DCH9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF78">
        <property type="entry name" value="OS12G0587133 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase zinc-binding" evidence="1">
        <location>
            <begin position="279"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9678729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="442" mass="51342" checksum="5217E4AA334A7841" modified="2024-01-24" version="1">MWFEAVSGLRINLEKSELIPVGRVENIDDLALDFGCRVGSLSSTYLGLPLGAPFKLVSVWDGVEERFCKRLTMWKRQYLSKGGRATLIRSTLSNLPIYFMSLLRLPSLVRRRLEQIQRDFLWGGGNLERKPHLVRWEMVCLSKKKGGLGVKCLSILNKALLSKWNWRYANEKEALWNQVIRGKYGENRGGWCSREVREVHGVGLWKGIRMDWELVDTQISFSVGNGRRVSFWRDRWCGDSPLCETFPSLFALAVEKEAWVAEVWDPLAEGGWGGLEPLCIWNGWVQPKISFFAWEAAWGKTLTLDLVQKRGWSLANRCFMCLENEETIDHLLLHCSKTRTLWELLFTLVGVSWVMPSSVRETLLSWHDSFVGKKRRKVWKTAPLHIFWTVWKARNRLTFKDNELSIQRLKYSFILSLWPEAKLFIVECSQSLVNFIDWLGSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DCY3</accession>
    <name>A0AA39DCY3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">alpha,alpha-trehalose-phosphate synthase (UDP-forming)</fullName>
            <ecNumber evidence="3">2.4.1.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_022587</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>D-glucose 6-phosphate + UDP-alpha-D-glucose = alpha,alpha-trehalose 6-phosphate + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18889"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58429"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="EC" id="2.4.1.15"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the trehalose phosphatase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the N-terminal section; belongs to the glycosyltransferase 20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.15" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DCY3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003825">
        <property type="term" value="F:alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004805">
        <property type="term" value="F:trehalose-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03788">
        <property type="entry name" value="GT20_TPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01627">
        <property type="entry name" value="HAD_TPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000010">
        <property type="entry name" value="Alpha,alpha-trehalose-phosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000052">
        <property type="entry name" value="Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000054">
        <property type="entry name" value="alpha,alpha-trehalose-phosphate synthase [UDP-forming] 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000017">
        <property type="entry name" value="alpha,alpha-trehalose-phosphate synthase [UDP-forming] 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001830">
        <property type="entry name" value="Glyco_trans_20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003337">
        <property type="entry name" value="Trehalose_PPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00685">
        <property type="entry name" value="T6PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788:SF55">
        <property type="entry name" value="ALPHA,ALPHA-TREHALOSE-PHOSPHATE SYNTHASE [UDP-FORMING] 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788">
        <property type="entry name" value="TREHALOSE-6-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00982">
        <property type="entry name" value="Glyco_transf_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02358">
        <property type="entry name" value="Trehalose_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9677692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="861" mass="97603" checksum="E8F608E0939C4EEE" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DD81</accession>
    <name>A0AA39DD81_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PVL29_021623</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DD81"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001023">
        <property type="entry name" value="Os07g0541700 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000178">
        <property type="entry name" value="serine/threonine-protein kinase PBS1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045874">
        <property type="entry name" value="LRK10/LRL21-25-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025287">
        <property type="entry name" value="WAK_GUB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27009">
        <property type="entry name" value="RUST RESISTANCE KINASE LR10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13947">
        <property type="entry name" value="GUB_WAK_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 16" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041415434" description="non-specific serine/threonine protein kinase" evidence="16">
        <location>
            <begin position="22"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="262"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ9679766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="549" mass="62196" checksum="6451A51D001FC3C3" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DEZ1</accession>
    <name>A0AA39DEZ1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF659 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_018405</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DEZ1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007021">
        <property type="entry name" value="DUF659"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32166:SF74">
        <property type="entry name" value="OS05G0256350 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32166">
        <property type="entry name" value="OSJNBA0013A04.12 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04937">
        <property type="entry name" value="DUF659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF659" evidence="1">
        <location>
            <begin position="3"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9682478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="85" mass="9703" checksum="1D4AECDACC6D0A6A" modified="2024-01-24" version="1">MEKKKNGCSIMSNGWTDEKERTLVNFLVNCSKGIMFMQSIDASSMIKTEEKILELLDKWVEQVGEKNVIQVITDNHSSYVMTGNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DH28</accession>
    <name>A0AA39DH28_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">GOLD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_019340</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the EMP24/GP25L family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DH28"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015720">
        <property type="entry name" value="Emp24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22811">
        <property type="entry name" value="TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01190">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041427225" description="GOLD domain-containing protein" evidence="9">
        <location>
            <begin position="21"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="178"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="10">
        <location>
            <begin position="30"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9683724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="210" mass="23766" checksum="BC74581FE2A7F977" modified="2024-01-24" version="1">MSRFKTLIVIVGLMCTAADSMRFDLTSGGTKCISEDIKTNAMSVGKYSVINPNEGFPLPDTHKITARVSSPYGNNYHTADHIESGNFAFTAAEDGDYTACFWALDHKPPLTVTIEFDWRTGVAAKDWSKVAKKGQVEVMELELKKLYDTVTSIHEEMFYLREREEEMQELNRATNSKMATFSFLSLLVCLSVAGLQLWHLKTFFERKKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DMZ4</accession>
    <name>A0AA39DMZ4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Subtilisin-like protease SBT1.8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PVL29_013917</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the peptidase S8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9687922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DMZ4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02120">
        <property type="entry name" value="PA_subtilisin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04852">
        <property type="entry name" value="Peptidases_S8_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.2310:FF:000001">
        <property type="entry name" value="Subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000006">
        <property type="entry name" value="Subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000005">
        <property type="entry name" value="subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.80:FF:000003">
        <property type="entry name" value="Subtilisin-like protease SBT1.9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034197">
        <property type="entry name" value="Peptidases_S8_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010259">
        <property type="entry name" value="S8pro/Inhibitor_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045051">
        <property type="entry name" value="SBT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041469">
        <property type="entry name" value="Subtilisin-like_FN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10795">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17766">
        <property type="entry name" value="fn3_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05922">
        <property type="entry name" value="Inhibitor_I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 11" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 11" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041423212" description="Subtilisin-like protease SBT1.8" evidence="13">
        <location>
            <begin position="22"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="domain" description="Inhibitor I9" evidence="16">
        <location>
            <begin position="24"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="14">
        <location>
            <begin position="130"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="15">
        <location>
            <begin position="372"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="Subtilisin-like protease fibronectin type-III" evidence="17">
        <location>
            <begin position="656"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="196"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="580"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="200"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="10 11">
        <location>
            <position position="138"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="10 11">
        <location>
            <position position="213"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="10 11">
        <location>
            <position position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF05922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF17766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ9687922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="20">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="762" mass="81061" checksum="7143B629C4047E85" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DNL0</accession>
    <name>A0AA39DNL0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF1771 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_012872</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DNL0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056254">
        <property type="entry name" value="At5g58720/SDE5-like_UBA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013899">
        <property type="entry name" value="DUF1771"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036063">
        <property type="entry name" value="Smr_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47872:SF3">
        <property type="entry name" value="NUCLEAR RNA EXPORT FACTOR SDE5 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47872">
        <property type="entry name" value="NUCLEAR RNA EXPORT FACTOR SDE5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08590">
        <property type="entry name" value="DUF1771"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24767">
        <property type="entry name" value="UBA_At5g58720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01162">
        <property type="entry name" value="DUF1771"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1771" evidence="2">
        <location>
            <begin position="339"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="223"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="224"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9690444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="500" mass="56192" checksum="68085D703B82EFD6" modified="2024-01-24" version="1">MEVSASSISEYDERKALEVLLDAFGSTFSLQEIAHAYCEAGRNADLAGEILYDMNGSISTSTTHASNQATATCNKEVKDDPSSECYSNISEHPCPADGNPKASKPKRRPVSVGTISGVIGKSYARCTASANESCVATKPLKLDMTNFPMFECQDKEDKFDSARDVHKDIEDFIFKMLGHGFQLDRGLIQEVLDNCGYDITKGMDKLLDMSAATLDETNSYLHDSSQKFTDDRPKSRSLMGQRKSEYMTGDRRILNTKEPELPGAKERRNLQKEVLAALFTTSERPEELPARTAKPRPVRKSRVAGNLVLEPFKDVEEYKIDDLFSKQIDEDADGEEDDSYQDLRQAVKEYRTTMKEYYKAAVNAFANGDRVKADKLLEQGHFFHNKAREADEESARKIFETRNVETEDEMSLDLHAHDAKEAILILKSHLSSLSGIPSIKFLKVIMETDEENISKGGARKRLIMKLLEKHSIKWTEGSNAGIILIRVDEINPQRLSFARK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DNM6</accession>
    <name>A0AA39DNM6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cytochrome P450 71D10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_013553</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DNM6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11072">
        <property type="entry name" value="CYP71-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000008">
        <property type="entry name" value="Cytochrome P450 71D8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47955:SF8">
        <property type="entry name" value="CYTOCHROME P450 71D11-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47955">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 71 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8 9" id="KW-0349">Heme</keyword>
    <keyword evidence="6 8 9" id="KW-0408">Iron</keyword>
    <keyword evidence="4 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041244929" description="Cytochrome P450 71D10" evidence="10">
        <location>
            <begin position="24"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="8">
        <location>
            <position position="442"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9691403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="505" mass="57153" checksum="A9684E4F4D5F8381" modified="2024-01-24" version="1">MEFSSSSLLFAFLLFLYMLYKIGKRSKANISTQKLPPGPWKLPLIGNVHQLVGSLPHRSLTLLAKKYGPLMRLQLGEVSTLIVSSPEMAKQVMKTHDTNFAQRPLLLATRILSYDCSGVAFSPYGDYWRQLRKICVVELLTAKRVKSFQSVREEEISNLITTVTSCSRLPINFTEEISSLAFSIIARAAFGKKSEDQDAFLSVMKEIVEMVSGFCVADMYPSVKWLDLISGMRYKLDKVFQRTDRMLQNVVGEHREKLKTQSGKLEGDEDLVDVLLKLQQNGDLQFPLTDNNIKAVILDIFGGAGESTSTSVEWAMSEMLKAPIVMEKAQAEVRSVFDGKGRVDETAIDELKFLKAVVNETLRLHPPFPLLLPRECREMCKINGYEIPEKTRIIVNAWAIGRDSDYWVEAERFYPERFLESSIDYKGTDFGYIPFGAGRRICPGILFAMPGIELPLANLLYHFDWKLPNGMKAEDLDMTEAFGLAVRRKQDLHLIPIPYNPSHAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DNU8</accession>
    <name>A0AA39DNU8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein ARV</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_012779</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Mediator of sterol homeostasis involved in sterol uptake, trafficking and distribution into membranes.</text>
    </comment>
    <comment type="function">
        <text evidence="10">Regulates also the sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Endoplasmic reticulum membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ARV1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DNU8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032366">
        <property type="term" value="P:intracellular sterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097036">
        <property type="term" value="P:regulation of plasma membrane sterol distribution"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006665">
        <property type="term" value="P:sphingolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016125">
        <property type="term" value="P:sterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007290">
        <property type="entry name" value="Arv1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14467">
        <property type="entry name" value="ARV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14467:SF0">
        <property type="entry name" value="PROTEIN ARV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04161">
        <property type="entry name" value="Arv1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 10" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="36"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU368065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9690295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="169" mass="19026" checksum="64567D93A13AA48F" modified="2024-01-24" version="1">MFIGEESHGSESTQLHYRVCLRSRQMPLVLAGLDCVLYKILMDVLFGNLAFLCIFLLATRNLLNTIGAVSRYKNLLLSILISSYFKIFFIAMMVWELSSSLIFIIDVFVLSSNAVALKVITGSGMSRCAETCFVAHAVKLFVSQLTGFHSSRFVQDLNIFRCEMPIYSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DQU0</accession>
    <name>A0AA39DQU0_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_009548</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9693638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DQU0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="98"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="147"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="180"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="213"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="245"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="302"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="333"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9693638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="391" mass="43845" checksum="45DAEA3E85ECB522" modified="2024-01-24" version="1">MCSFPQLSSPKDSSGLIPPTFFSQLPPPIFQYRIMDSNNEERLPSSEVEGKNALKGRIWEESKKTLRIAFPTMLFRVASFGMAVVTQLFVGHFGQIELVAYALIQTILVLFVSGILQGMSSATETLCGQAFGAKQYHMMGIYLQRTWIVNLIAAKIMLPLFIFATPIFRLLGQAEEISTVAGKISLWFIPYVYYLLFSRTIQRYLQAQLKNMVVGWLSAFSFVFHVLLSWIFVSKLKLGIPGAMSALNISSWLVVIAQFVYVLGGWCPETWKGFTTAAFANLLPVIKLSISSGVMLCLNINAWALMFFLGFLGAVCVRVSNELGKGNARAAKFAVKGMWIGMLCGVAMQLLVLFYLTWRTNWDEQVRKTAERLNKWSLKPPEEPNQNPPQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DR59</accession>
    <name>A0AA39DR59_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">PVL29_013399</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="22">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DR59"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033612">
        <property type="term" value="F:receptor serine/threonine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009791">
        <property type="term" value="P:post-embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000317">
        <property type="entry name" value="Inactive leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000233">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase TDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000041">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000358">
        <property type="entry name" value="Putative leucine-rich repeat receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000661">
        <property type="entry name" value="Serine-threonine protein kinase plant-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050647">
        <property type="entry name" value="Plant_LRR-RLKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056:SF73">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE EFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="15 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="142"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="746"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="803"/>
            <end position="1079"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAJ9691207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1083" mass="119773" checksum="D024517DE345C03F" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DTV9</accession>
    <name>A0AA39DTV9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Autophagy-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PVL29_008805</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Ubiquitin-like modifier involved in autophagosomes formation. May mediate the delivery of the autophagosomes to the vacuole via the microtubule cytoskeleton.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
            <location evidence="3">Autophagosome membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Vacuole membrane</location>
            <topology evidence="4">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 18">Belongs to the ATG8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DTV9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000421">
        <property type="term" value="C:autophagosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16108">
        <property type="entry name" value="Ubl_ATG8_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000235">
        <property type="entry name" value="Autophagy-related protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004241">
        <property type="entry name" value="Atg8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10969">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEINS 1A/1B LIGHT CHAIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02991">
        <property type="entry name" value="ATG8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 18" id="KW-0072">Autophagy</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="14" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="15 17" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0493">Microtubule</keyword>
    <keyword evidence="11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0926">Vacuole</keyword>
    <feature type="lipid moiety-binding region" description="Phosphatidylserine amidated glycine; alternate" evidence="17">
        <location>
            <position position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR604241-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU004384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ9696773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="129" mass="15017" checksum="F0E8592C58EB2133" modified="2024-01-24" version="1">MGKKSMSSFKDEFSYEERLEESRDIIAKYPDRVPVIAERYSKTDLPDMEKKKFLVPRDMSVGQFIHILSIRLHLTPGKALFVFVENTLPQTASLMDTVYESFKDEDGFLYMSYSSEKTFGYTDIQIHGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DVR1</accession>
    <name>A0AA39DVR1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_008158</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9699431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DVR1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33701:SF3">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR ATRX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33701">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="124"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="197"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="302"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="478"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="23"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="134"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="197"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="226"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="326"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="532"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9699431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="542" mass="59478" checksum="8F92613400D7ED4E" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DY48</accession>
    <name>A0AA39DY48_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">O-fucosyltransferase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_006490</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycan metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase GT106 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DY48"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006004">
        <property type="term" value="P:fucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11299">
        <property type="entry name" value="O-FucT_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024709">
        <property type="entry name" value="FucosylTrfase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019378">
        <property type="entry name" value="GDP-Fuc_O-FucTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31741:SF14">
        <property type="entry name" value="O-FUCOSYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31741">
        <property type="entry name" value="OS02G0726500 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10250">
        <property type="entry name" value="O-FucT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="11" id="KW-0294">Fucose metabolism</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="478"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="483"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9701168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="499" mass="56651" checksum="713A30A0C3231FD0" modified="2024-01-24" version="1">MRRYGHHRQPKHQSGGGLKAVLGRVSVAVAALLICTVWLLFSSSSVSNGNRSFRYGSKVTPPSESNGYLRVRCNGGLNQQRTAICNAVLAARIMNATLVLPELDANSFWHDSSGFQGIYDVEHFIAVLRFDVRIVESIPETRKNGKTKKIKAYQLRPPRDAPISWYTTDALKKMKEHGAIYLTPFSHRLAEEIDNPEYQRLRCRVNYHALRFKPHIMKLSNTIVNKLRAQGHFMSIHLRFEMDMLAFAGCLDIFTPEEQRTLTKYRKENFAAKRLVYHERRAIGKCPLTPEEVGLVLHALGFDNSTKIYLAAGELFGGDRFMKPFRALFPRLENHSSVEPADELLENSQGLVGSAVDYMVCLLSDIFMPTYDGPSNFANNLLGHRLYYGFRTTLRPDRKALAPIFIDRENGRLAGFEEAVRRVMLNTNFGGPHKRVPPESFYTNSWPECFCQMSPKNPADKCPPENVLEILNNRLESEMGNGFESTTTSNLTVPREEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E067</accession>
    <name>A0AA39E067_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">diphosphoinositol-polyphosphate diphosphatase</fullName>
            <ecNumber evidence="1">3.6.1.52</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_004881</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>1,5-bis(diphospho)-1D-myo-inositol 2,3,4,6-tetrakisphosphate + H2O = 1-diphospho-1D-myo-inositol 2,3,4,5,6-pentakisphosphate + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:79699"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:74946"/>
            <dbReference type="ChEBI" id="CHEBI:77983"/>
            <dbReference type="EC" id="3.6.1.52"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:79700"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>3,5-bis(diphospho)-1D-myo-inositol 1,2,4,6-tetrakisphosphate + H2O = 3-diphospho-1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:56312"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:140372"/>
            <dbReference type="ChEBI" id="CHEBI:140374"/>
            <dbReference type="EC" id="3.6.1.52"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:56313"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>5-diphospho-1D-myo-inositol 1,2,3,4,6-pentakisphosphate + H2O = 1D-myo-inositol hexakisphosphate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22384"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58130"/>
            <dbReference type="ChEBI" id="CHEBI:58628"/>
            <dbReference type="EC" id="3.6.1.52"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:22385"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>6-diphospho-1D-myo-inositol pentakisphosphate + H2O = 1D-myo-inositol hexakisphosphate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:79703"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58130"/>
            <dbReference type="ChEBI" id="CHEBI:230534"/>
            <dbReference type="EC" id="3.6.1.52"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:79704"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein-tyrosine phosphatase family. Atypical dual-specificity phosphatase Siw14-like subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.52" evidence="1"/>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E067"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052847">
        <property type="term" value="F:inositol-1,5-bisdiphosphate-2,3,4,6-tetrakisphosphate 5-diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052845">
        <property type="term" value="F:inositol-5-diphosphate-1,2,3,4,6-pentakisphosphate diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14528">
        <property type="entry name" value="PFA-DSP_Siw14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000024">
        <property type="entry name" value="probable tyrosine-protein phosphatase At1g05000"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020428">
        <property type="entry name" value="PFA-DSPs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004861">
        <property type="entry name" value="Siw14-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126:SF46">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE DSP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03162">
        <property type="entry name" value="Y_phosphatase2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01911">
        <property type="entry name" value="PFDSPHPHTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="8">
        <location>
            <begin position="20"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00044949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9703268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="202" mass="23290" checksum="3BF059D37E159E13" modified="2024-01-24" version="1">MGLMLENGVANDAVYVPPSNFSMVEENIFRSGLPSPINFPFLETLNLRSIIYLCPEPYPEENCKFLQSQNIRLFQFGIEGKKEPPVAMSTDTISEALKVLIDVRNHPILIHCKRGKHRTGCLVGCLRKLQNWCLSSVIEEYQRFAGAKSRINDMKFMETYDILSMRQSLYSIIYRYQGYGSNKRRLLYRGEENVHKARLASI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E153</accession>
    <name>A0AA39E153_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small auxin up regulated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_002731</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ARG7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E153"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009733">
        <property type="term" value="P:response to auxin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003676">
        <property type="entry name" value="SAUR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31929">
        <property type="entry name" value="SAUR-LIKE AUXIN-RESPONSIVE PROTEIN FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02519">
        <property type="entry name" value="Auxin_inducible"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9704303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="166" mass="18997" checksum="C75E3A83C290F67C" modified="2024-01-24" version="1">MAIRFHRIIPAKQILRRILPSPEPTNVPKGYVPVYVGETQKKRFVIPISYLKHPSFQNLLSQAEEEFGLKLMAIRFQRIIPAKQILRRILPSPEPTNVPKGYVPVYVGETQKKRFVIPISYLKHPSFQNLLSQAEEEFGFDHPLGGLTIPCREEAFVNLTCSLNCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E2F0</accession>
    <name>A0AA39E2F0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NADP-dependent glyceraldehyde-3-phosphate dehydrogenase</fullName>
            <ecNumber evidence="6">1.2.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Glyceraldehyde-3-phosphate dehydrogenase [NADP(+)]</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Non-phosphorylating glyceraldehyde 3-phosphate dehydrogenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Triosephosphate dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_004386</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>D-glyceraldehyde 3-phosphate + NADP(+) + H2O = (2R)-3-phosphoglycerate + NADPH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:14669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="1.2.1.9"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.9" evidence="6"/>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E2F0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008886">
        <property type="term" value="F:glyceraldehyde-3-phosphate dehydrogenase (NADP+) (non-phosphorylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008911">
        <property type="term" value="F:lactaldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07082">
        <property type="entry name" value="ALDH_F11_NP-GAPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000016">
        <property type="entry name" value="NADP-dependent glyceraldehyde-3-phosphate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000013">
        <property type="entry name" value="NADP-dependent glyceraldehyde-3-phosphate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051020">
        <property type="entry name" value="ALDH-related_metabolic_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42991">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42991:SF1">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="5 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="14">
        <location>
            <begin position="24"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00042470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00042646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00043052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9702622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="496" mass="53099" checksum="1DADBA5840630B64" modified="2024-01-24" version="1">MAGSGVFAEILDGDVFKYYTDGAWKTSSSGKSVPIINPTTRSTQYKVQACTQEEVNKVMETAKTAQKLWAKTPLWKRAELLHKAAAILKEQKAPIAECLVKEIAKPAKDAVTEVVRSGDLVSYCAEEGVRILGEGKFLVSDSFPGNERSKYCLTSKIPLGVILAIPPFNYPVNLAVSKIGPALIAGNSLVLKPPTQGAVAALHMVHCFHLAGFPKGLINCVTGRGSEIGDFLTMHPGVDCISFTGGDTGIAISKKAGMVPLQMELGGKDACIILEDADLDLVASSIVKGGFSYSGQRCTAIKVVLVMESVADALVEKVNAKLAKLTVGAPEDDCDITPVVTESSANFIEGLVTDAKQKEATFCQEYKREGNLIYPLLLDNVRPDMRIAWEEPFGPVLPVIRINSVEEGIHHCNASNFGLQGCVFTRDINKAILIGDAMETGTVQINSAPARGPDHFPFQGFKDSGIGSQGITNSINMMTKTKSMVINLPTPTYSMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E3Y8</accession>
    <name>A0AA39E3Y8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Methanol O-anthraniloyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_002539</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E3Y8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050898">
        <property type="entry name" value="Plant_acyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31147">
        <property type="entry name" value="ACYL TRANSFERASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31147:SF66">
        <property type="entry name" value="OS05G0315700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9707543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="443" mass="49748" checksum="A1509EE4C682E871" modified="2024-01-24" version="1">MASSSSPLVFSVKRCDPEFVRPTNPTPREVKQLSDIDDQEGLRFQLPVIMFYPNNPLMKGKDPVKVIREALGKALVYYYPFAGRLIEGNNRKLMVDCTGEGILFIEADADTTLENLGDAIQPMCPCFEELLYDVPGSGEFLVTRLRCGGFIFALRLNHTMSDALGLIQFLNAISEMAQGLSVPSLLPIWQRELLNARNPPQITRIHHEYEEVTNSKGTLMAMDENNLVHRSFFFGPKQIRALRNRLPASLGACSTFEVLTAYVWRCRTIAFAVDPDEVVRISCLINMRGKHGFYLPPGYYGNAFVYPASITKAGMLCKNPLEYAIRLLKKAKAEMSQEYIKSVADLMVIKGRPLFTQPGNYFVSDVTRAGFGEVNFGWGKPVYGGLARALSIISFCTRFRNSKGEEGNVIPICLPPPVMERFEQELKRMTKEAEPVRLITSML</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E493</accession>
    <name>A0AA39E493_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Serine/threonine-protein kinase ATR</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PVL29_002871</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PI3/PI4-kinase family. ATM subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00892">
        <property type="entry name" value="PIKKc_ATR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1070.11:FF:000024">
        <property type="entry name" value="Serine/threonine-protein kinase ATR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1010.10:FF:000036">
        <property type="entry name" value="Serine/threonine-protein kinase ATR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056802">
        <property type="entry name" value="ATR-like_M-HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050517">
        <property type="entry name" value="DDR_Repair_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003152">
        <property type="entry name" value="FATC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057564">
        <property type="entry name" value="HEAT_ATR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003151">
        <property type="entry name" value="PIK-rel_kinase_FAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014009">
        <property type="entry name" value="PIK_FAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012993">
        <property type="entry name" value="UME"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139">
        <property type="entry name" value="ATAXIA TELANGIECTASIA MUTATED ATM -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139:SF69">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE ATR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02259">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02260">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23593">
        <property type="entry name" value="HEAT_ATR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25030">
        <property type="entry name" value="M-HEAT_ATR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08064">
        <property type="entry name" value="UME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01343">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00802">
        <property type="entry name" value="UME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51189">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51190">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FAT" evidence="17">
        <location>
            <begin position="1664"/>
            <end position="2277"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="16">
        <location>
            <begin position="2387"/>
            <end position="2700"/>
        </location>
    </feature>
    <feature type="domain" description="FATC" evidence="18">
        <location>
            <begin position="2698"/>
            <end position="2730"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ9704512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="2730" mass="306220" checksum="B85505B5D041DC30" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E557</accession>
    <name>A0AA39E557_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_002125</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E557"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34189">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34189:SF13">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="109"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9707005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="136" mass="15059" checksum="3BA37679CD57B79B" modified="2024-01-24" version="1">MHRISSATRVSDEFFNSTTSPSATPDADQLPTYDPLSHVAKKERTRIRSAENAIHAIPLVLLLCVITLWFFSNPAVDIVNKGASIVPRQKGSTVNGNIKSDLTQNKLLKDLEQEDSDPTKELLHQKGPKSSENESY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E5W9</accession>
    <name>A0AA39E5W9_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PVL29_001554</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RBOH (TC 5.B.1.3) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E5W9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016174">
        <property type="term" value="F:NAD(P)H oxidase H2O2-forming activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06186">
        <property type="entry name" value="NOX_Duox_like_FAD_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000049">
        <property type="entry name" value="Respiratory burst oxidase homolog A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000019">
        <property type="entry name" value="Respiratory burst oxidase homolog A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.80:FF:000007">
        <property type="entry name" value="Respiratory burst oxidase protein A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000778">
        <property type="entry name" value="Cyt_b245_heavy_chain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013112">
        <property type="entry name" value="FAD-bd_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013130">
        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013121">
        <property type="entry name" value="Fe_red_NAD-bd_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013623">
        <property type="entry name" value="NADPH_Ox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050369">
        <property type="entry name" value="RBOH/FRE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11972">
        <property type="entry name" value="NADPH OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11972:SF153">
        <property type="entry name" value="SUPEROXIDE-GENERATING NADPH OXIDASE HEAVY CHAIN SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08022">
        <property type="entry name" value="FAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08030">
        <property type="entry name" value="NAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08414">
        <property type="entry name" value="NADPH_Ox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00466">
        <property type="entry name" value="GP91PHOX"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01169">
        <property type="entry name" value="NADPH_oxidase_subgroup_(NOX)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0274">FAD</keyword>
    <keyword evidence="5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="13 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0521">NADP</keyword>
    <keyword evidence="12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="383"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="522"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="563"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="742"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="16">
        <location>
            <begin position="259"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="17">
        <location>
            <begin position="616"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="62"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="778"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="23"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="65"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="75"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="778"/>
            <end position="796"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ9706042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="943" mass="106907" checksum="D00CC161FB7A5AAF" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YHV0</accession>
    <name>A0AA38YHV0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_026948</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YHV0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010228">
        <property type="term" value="P:vegetative to reproductive phase transition of meristem"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000309">
        <property type="entry name" value="E3 ubiquitin-protein ligase MBR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045191">
        <property type="entry name" value="MBR1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22937">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF165"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22937:SF209">
        <property type="entry name" value="RING-TYPE E3 UBIQUITIN TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="174"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9670724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="220" mass="24204" checksum="69C7D304328E6892" modified="2024-01-24" version="1">MVVYQLLRLLGQDVLVDSPSHQAGSNSAPAHQAASILELRTQPPTNLAPVSEAAASLAPATWLPPYLPALHAATSLAPANWLPAYLEPADQVENNFAPVSWDENDFDEEEEDDDYNNEDMRADVDNMSYEELLALEDHIGNVSTGLSEDAIKAKLKPKKYSSFTMGPPVEDEICCICQEEYEEGETMGILDCGHEFHSDCIKQWLVQKNLCPICKTAALV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YIW0</accession>
    <name>A0AA38YIW0_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_027305</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9671255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YIW0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.2700:FF:000001">
        <property type="entry name" value="Transmembrane protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056284">
        <property type="entry name" value="AIR9-like_A9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055474">
        <property type="entry name" value="DUF7046"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31149">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31149:SF10">
        <property type="entry name" value="OS05G0100900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23080">
        <property type="entry name" value="DUF7046"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23197">
        <property type="entry name" value="IG_AIR9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIR9-like A9" evidence="4">
        <location>
            <begin position="526"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7046" evidence="3">
        <location>
            <begin position="643"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="134"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="376"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="37"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="189"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="142"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="379"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="437"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="450"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="479"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="492"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9671255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="746" mass="82361" checksum="FBC396AB78978FA2" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YKH7</accession>
    <name>A0AA38YKH7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="8">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_025664</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="domain">
        <text evidence="8">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DEAD box helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="8"/>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YKH7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd17941">
        <property type="entry name" value="DEADc_DDX10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025313">
        <property type="entry name" value="SPB4-like_CTE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13959">
        <property type="entry name" value="CTE_SPB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01178">
        <property type="entry name" value="DUF4217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="12">
        <location>
            <begin position="70"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="101"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="298"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="643"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="6">
        <location>
            <begin position="70"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="643"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="666"/>
            <end position="678"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9672107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="746" mass="83855" checksum="D2CA48F284D69A4C" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YL91</accession>
    <name>A0AA38YL91_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_025913</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YL91"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000009">
        <property type="entry name" value="transcription factor MYB1R1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006447">
        <property type="entry name" value="Myb_dom_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01557">
        <property type="entry name" value="myb_SHAQKYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44042:SF15">
        <property type="entry name" value="DUPLICATED HOMEODOMAIN-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44042">
        <property type="entry name" value="DUPLICATED HOMEODOMAIN-LIKE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="66"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="7">
        <location>
            <begin position="66"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="8">
        <location>
            <begin position="69"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="117"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="117"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="142"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9672486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="151" mass="17581" checksum="CFAC7450CE770E40" modified="2024-01-24" version="1">MFAEETPDRWYKISNQIPGKSTIDVLEHYIRLIQDTDAIDFGAMDRYIPSMWNLEEVRAEEGSDSKEKKKGTSWTEEEHILFLQGLIKYGKGDWKNICRNYVTTKTPTQVASHAQKYFERQKRGNEEKKRKRSSIHDITINDIPSSHGSDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YLB4</accession>
    <name>A0AA38YLB4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">NB-ARC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_025940</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YLB4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF1205">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RPM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0611">Plant defense</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NB-ARC" evidence="3">
        <location>
            <begin position="11"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="4">
        <location>
            <begin position="164"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="5">
        <location>
            <begin position="282"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9672531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="515" mass="58562" checksum="6960E369AA27D666" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YLD2</accession>
    <name>A0AA38YLD2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_025967</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. PCMP-H subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YLD2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000690">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000348">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF529">
        <property type="entry name" value="TETRATRICOPEPTIDE-LIKE HELICAL DOMAIN SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="86"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="121"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="187"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="218"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="322"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9672561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="530" mass="58955" checksum="D03B61EB66D138FE" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YLR4</accession>
    <name>A0AA38YLR4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_026128</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YLR4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039301">
        <property type="entry name" value="Sip5/DA2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31315">
        <property type="entry name" value="PROTEIN SIP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31315:SF1">
        <property type="entry name" value="PROTEIN SIP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9672777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="340" mass="37399" checksum="FF2E3BE327CE76C1" modified="2024-01-24" version="1">MGNKLGRRRQVVEDKYTRPQGLYQHKDVDHKKLRKLILDSKLAPCYPGDEESTNDFEECPICFLFYPSLNRSRCCTKGICTECFLQMKNPNSTRPTQCPYCKTANYAVEYRGVKTKEEKGMEQIGFDLDLEDIMVMEAIWLSIQDNNGRHRNPLYGDTTTAEQYVTEEHYVVPAMAPQVESSSSPSGGLACAIAALAERQQMGGESSTNYNGNMPAFNMPPGSSRFSNRVEQYPENYPPIESSMNALPDGGLAVTKDDGEWGVDRVSEVAEAGTSYASSDATDEAGGVAALPPTDEAEGSFQNVGGPIVPESFEEQMMLAMAVSLAEARARTSTQGVSWQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2025-04-02" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YPZ4</accession>
    <name>A0AA38YPZ4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CST complex subunit CTC1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_023836</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CTC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990879">
        <property type="term" value="C:CST complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042162">
        <property type="term" value="F:telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045740">
        <property type="term" value="P:positive regulation of DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010833">
        <property type="term" value="P:telomere maintenance via telomere lengthening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042617">
        <property type="entry name" value="CTC1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028262">
        <property type="entry name" value="CTC1_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14865">
        <property type="entry name" value="CST COMPLEX SUBUNIT CTC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14865:SF2">
        <property type="entry name" value="CST COMPLEX SUBUNIT CTC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15491">
        <property type="entry name" value="CTC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0779">Telomere</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="23"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="23"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9674520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1365" mass="151376" checksum="DF92DDF3E7D3FE49" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YQ25</accession>
    <name>A0AA38YQ25_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cytochrome P450 82A3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_023855</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YQ25"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20654">
        <property type="entry name" value="CYP82"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000026">
        <property type="entry name" value="Cytochrome P450 82C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050651">
        <property type="entry name" value="Plant_Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47947:SF39">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47947">
        <property type="entry name" value="CYTOCHROME P450 82C3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7 8" id="KW-0349">Heme</keyword>
    <keyword evidence="5 7 8" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 8" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="229"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="7">
        <location>
            <position position="463"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9674565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="525" mass="58882" checksum="CF8FFF364D45ACCE" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YRY8</accession>
    <name>A0AA38YRY8_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_024479</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YRY8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008511">
        <property type="entry name" value="ROH1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47467">
        <property type="entry name" value="OS01G0867200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47467:SF1">
        <property type="entry name" value="WD40 REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05633">
        <property type="entry name" value="ROH1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="605"/>
            <end position="627"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9675571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="754" mass="84166" checksum="487BC1E22D74B688" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YS20</accession>
    <name>A0AA38YS20_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_024520</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GASA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YS20"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003854">
        <property type="entry name" value="GASA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23201">
        <property type="entry name" value="EXTENSIN, PROLINE-RICH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23201:SF12">
        <property type="entry name" value="OS05G0432200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02704">
        <property type="entry name" value="GASA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9675633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="80" mass="8858" checksum="0ACB4C48C6F51108" modified="2024-01-24" version="1">MKSISLTNILSHNFVLYELSDCKSKCAYRCSKAGWHKLCLRACNTCCQRCNCVPPGTAGNEDVCPCYAKMTTHGGRHKCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YSQ3</accession>
    <name>A0AA38YSQ3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DYW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_024788</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. PCMP-H subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YSQ3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000227">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At3g13880"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000776">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At3g13880"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001495">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At3g13880"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032867">
        <property type="entry name" value="DYW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046849">
        <property type="entry name" value="E2_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF381">
        <property type="entry name" value="DYW DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14432">
        <property type="entry name" value="DYW_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20430">
        <property type="entry name" value="Eplus_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041416054" description="DYW domain-containing protein" evidence="4">
        <location>
            <begin position="26"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="42"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="143"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="215"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="352"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="453"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="domain" description="DYW" evidence="5">
        <location>
            <begin position="684"/>
            <end position="760"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF14432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9675978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="760" mass="84793" checksum="23862BE09DB1CB87" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YSS2</accession>
    <name>A0AA38YSS2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein NRT1/ PTR FAMILY 5.10-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_024753</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YSS2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009705">
        <property type="term" value="C:plant-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071916">
        <property type="term" value="F:dipeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080054">
        <property type="term" value="F:low-affinity nitrate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042937">
        <property type="term" value="F:tripeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17417">
        <property type="entry name" value="MFS_NPF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000147">
        <property type="entry name" value="Protein NRT1/ PTR family 5.10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044739">
        <property type="entry name" value="NRT1/PTR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000109">
        <property type="entry name" value="POT_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018456">
        <property type="entry name" value="PTR2_symporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11654">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00854">
        <property type="entry name" value="PTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01022">
        <property type="entry name" value="PTR2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="104"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="147"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="222"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="329"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="368"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="411"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="463"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="494"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="539"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9675935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="570" mass="62233" checksum="9CE0BDAC5A8818A0" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YV65</accession>
    <name>A0AA38YV65_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_022219</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YV65"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9677112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="100" mass="11815" checksum="219F643BA529D2B4" modified="2024-01-24" version="1">MLFLSLFGVSWVFLFSVKETLLGWRGSFVGKKRKKVWQVGPLCLFWVIWKVRNRIAFEDSVLSIQRLKTSFVYLLWLETKLWIKDGPSTLLDFVNWVGSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YVK2</accession>
    <name>A0AA38YVK2_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_022307</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YVK2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00265">
        <property type="entry name" value="MADS_MEF2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050142">
        <property type="entry name" value="MADS-box/MEF2_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033896">
        <property type="entry name" value="MEF2-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002487">
        <property type="entry name" value="TF_Kbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48019">
        <property type="entry name" value="SERUM RESPONSE FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01486">
        <property type="entry name" value="K-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00404">
        <property type="entry name" value="MADSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51297">
        <property type="entry name" value="K_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00350">
        <property type="entry name" value="MADS_BOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="MADS-box" evidence="8">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="K-box" evidence="9">
        <location>
            <begin position="124"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="161"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9677234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="282" mass="32000" checksum="B02E2168BB6665E7" modified="2024-01-24" version="1">MGRGRVELKRIENKINRQVTFAKRRNGLLKKAYELSVLCDAEVALIIFSSRGKLYEFCSGPGYFLLFISLSFALDLLHLYALDLTLSVKPSSLVAVKDLMAKTLEKYQKCSYGALEASQPVNEPTQSYQEYLKLKTRVEVLQRSQRNLLGEDLDPLNTKELEQLEHQLEMSLKQVRSTKTQNMLDQLADLQNKEHMLIEANNALRRKLEESNGKYPLQQSWEAAGNNALYSRLPAQSEGFFQPLERNSTLEMGYNAAGSNEITLAAPSQNDNGFGPGWMLWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YWK1</accession>
    <name>A0AA38YWK1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pentatricopeptide repeat-containing protein At1g64310</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_022684</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the PPR family. PCMP-E subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YWK1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000529">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000343">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At3g58590"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000797">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001811">
        <property type="entry name" value="Putative pentatricopeptide repeat-containing protein At1g64310"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001184">
        <property type="entry name" value="Putative pentatricopeptide repeat-containing protein At3g18840"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF452">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="68"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="169"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="270"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="371"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="406"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00061659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9677852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="554" mass="61365" checksum="76903FECC3AF514F" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YX34</accession>
    <name>A0AA38YX34_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Late embryogenesis abundant protein LEA-2 subgroup domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_020459</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YX34"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004864">
        <property type="entry name" value="LEA_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044839">
        <property type="entry name" value="NDR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31234">
        <property type="entry name" value="LATE EMBRYOGENESIS ABUNDANT (LEA) HYDROXYPROLINE-RICH GLYCOPROTEIN FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31234:SF72">
        <property type="entry name" value="NDR1_HIN1-LIKE PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03168">
        <property type="entry name" value="LEA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="67"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="Late embryogenesis abundant protein LEA-2 subgroup" evidence="7">
        <location>
            <begin position="122"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9678303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="250" mass="28140" checksum="D1776596386B684C" modified="2024-01-24" version="1">MADHQKIHPVVDPEAPPTEPLVPRGSFRSEKGDPVQHPPDPPPLQRTSPVIQSKPPKRSCCCKCMCWTISLLVLLLVIVGAIAGVLYLVFRPKLPKYSVDSLRITDFRLNYDLTLYAKFDMKIRANNPNKKIGIYYEKGGKLSVWYTNNKLCQGSLPKFYQGHQNTTVLNVVLTGQTQYGSTLMTALQEQQQKGRIPLDLKVDVPVAIKLGKLKLKKVRFLVECLLLVDSLSANNLISIRASDCRFRLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YX43</accession>
    <name>A0AA38YX43_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF4283 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_022878</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9678117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YX43"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34427">
        <property type="entry name" value="DUF4283 DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34427:SF5">
        <property type="entry name" value="DUF4283 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="349"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="548"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="621"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="381"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9678117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="734" mass="81096" checksum="A0911B3AC97AE3EC" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YYI4</accession>
    <name>A0AA38YYI4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pectinesterase inhibitor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_021075</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the PMEI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YYI4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046910">
        <property type="term" value="F:pectinesterase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd15798">
        <property type="entry name" value="PMEI-like_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.40:FF:000005">
        <property type="entry name" value="Pectin methylesterase inhibitor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.40">
        <property type="entry name" value="Invertase/pectin methylesterase inhibitor family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035513">
        <property type="entry name" value="Invertase/methylesterase_inhib"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006501">
        <property type="entry name" value="Pectinesterase_inhib_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051955">
        <property type="entry name" value="PME_Inhibitor"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01614">
        <property type="entry name" value="PME_inhib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31080:SF161">
        <property type="entry name" value="OS10G0508700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31080">
        <property type="entry name" value="PECTINESTERASE INHIBITOR-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04043">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00856">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101148">
        <property type="entry name" value="Plant invertase/pectin methylesterase inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Pectinesterase inhibitor" evidence="3">
        <location>
            <begin position="47"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9679041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="215" mass="23177" checksum="024FD8D6E2BB9C64" modified="2024-01-24" version="1">MSTEMESFTCLKLSSSRGLAAIVALFFFYLSLTTCSAASPEPQSPTNTIEFIRTSCGVTMYPKLCFKTLSAYASTIQTSPMELASAALNVSLKGAQSSSNKVLNLSKGQGLSRREAAAITDCIENMQDSVDELQQSLVAMKDLQGPDFQMKMSDIVTWVSAALTDEDTCMDGFEGHAMKGELKSTIRSYIVSVAQLTSNALAIINKFLSTQGNQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z126</accession>
    <name>A0AA38Z126_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_019678</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the NDUFAF3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z126"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05125">
        <property type="entry name" value="Mth938_2P1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1230.10:FF:000003">
        <property type="entry name" value="NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1230.10">
        <property type="entry name" value="MTH938-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036748">
        <property type="entry name" value="MTH938-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034095">
        <property type="entry name" value="NDUF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007523">
        <property type="entry name" value="NDUFAF3/AAMDC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21192:SF2">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX ASSEMBLY FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21192">
        <property type="entry name" value="NUCLEAR PROTEIN E3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04430">
        <property type="entry name" value="DUF498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64076">
        <property type="entry name" value="MTH938-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00049984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9680421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="172" mass="19139" checksum="BBBE1909EF78AFE4" modified="2024-01-24" version="1">MVVRQRAVATLPTLMRALRKEPPKPTTQPLPSLRRAFSLYDQINLIDNVPEDQLRFQGYTDTGFTVSGVQYEGSLLCVGNVLLSWGPKKLSEITADSLSIFRIVRPIPEILILGCGRHIEQVDPELRRYIQSTGMKLEAVDSRNAASTYNILNEEGRIVAAALLPYGVSRDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z1D0</accession>
    <name>A0AA38Z1D0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SHSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_019663</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z1D0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd06472">
        <property type="entry name" value="ACD_ScHsp26_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.790:FF:000009">
        <property type="entry name" value="17.6 kDa class I heat shock protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031107">
        <property type="entry name" value="Small_HSP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11527">
        <property type="entry name" value="HEAT-SHOCK PROTEIN 20 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="6">
        <location>
            <begin position="46"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9680402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="160" mass="17948" checksum="F889D6F5D6775B1F" modified="2024-01-24" version="1">MSLIPSVLGGGRRSSIFDPFSLDIWDPFEGFPFSTTLSNIPSTVGETSAFANTRIDWKETPVAHVFKADLPGLKKEEVKVEVEEGRVLQISGERSKEQEEKNDKWHRVERSSGKFLRRFRLPEDAKTDEVKASMENGVLTVTVPKEEVKKAEVKAIEISG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z1L2</accession>
    <name>A0AA38Z1L2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="1">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_019682</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="1"/>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z1L2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047129">
        <property type="entry name" value="PPA2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45619">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE PP2A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="5">
        <location>
            <begin position="45"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9680428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="312" mass="35541" checksum="5D2A0E628163E392" modified="2024-01-24" version="1">MDPHHLDVENLDFGIDACNEFSPFEDDETPTFSSLLSEIFHVRCICHIINLILLDGLKLISPSLQAIHTAKHKTFVCSVIVNLYDEVCGLYCPDTNYLFMGDYVGRGYYSIETVTLLVALKVHYPQRITILGGNHESRQYGNANVWKIFTDLFDYFPLTALVESEIFCLPGGLSPSIDILDNIRNFDRVQEVPHEGPMCDLLWSDPDDRCGWGISPRGAGYTFGQDISEQFNHTNNLKLIARAHQLVMEGFNWDHEQKVVTIFSAPNYCYRCGNMASILEVDDCKGHTFIQFEPAPRRAEPDVTRRTPDYFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z1V1</accession>
    <name>A0AA38Z1V1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_019787</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z1V1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001305">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At1g14470"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000941">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At5g15300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000184">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF437">
        <property type="entry name" value="PENTACOTRIPEPTIDE-REPEAT REGION OF PRORP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12854">
        <property type="entry name" value="PPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="163"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="194"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="225"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="287"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="389"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="424"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9680577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="618" mass="69728" checksum="427681D7ADA087E8" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z1W6</accession>
    <name>A0AA38Z1W6_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_019932</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z1W6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039312">
        <property type="entry name" value="ZPR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33601:SF22">
        <property type="entry name" value="PROTEIN LITTLE ZIPPER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33601">
        <property type="entry name" value="PROTEIN LITTLE ZIPPER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9680764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="111" mass="13359" checksum="A047488FB1DBEDDC" modified="2024-01-24" version="1">MCMCPELLQSLPRCSTVFRGRRLKRFNVQVRRLNRMRSRRTRKESKDKAMMVVGLKSDMEIKNWKLYMLNQSIIQENEKLRKKALLLHQENQALLSQLENKFKTSPHNNNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z534</accession>
    <name>A0AA38Z534_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_018322</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z534"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000558">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At5g39710"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47938:SF7">
        <property type="entry name" value="PENTACOTRIPEPTIDE-REPEAT REGION OF PRORP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47938">
        <property type="entry name" value="RESPIRATORY COMPLEX I CHAPERONE (CIA84), PUTATIVE (AFU_ORTHOLOGUE AFUA_2G06020)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12854">
        <property type="entry name" value="PPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="13"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="98"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="133"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="168"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="203"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="238"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="273"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="308"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="343"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="378"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="413"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="448"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="483"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="518"/>
            <end position="552"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9682382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="582" mass="65779" checksum="40A0FD4B419E4826" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z7B4</accession>
    <name>A0AA38Z7B4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Single-stranded DNA binding protein Ssb-like OB fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_019285</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z7B4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048970">
        <property type="entry name" value="OB_Ssb-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31472:SF39">
        <property type="entry name" value="F5O8.30 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31472">
        <property type="entry name" value="OS05G0244600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21473">
        <property type="entry name" value="OB_Ssb-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Single-stranded DNA binding protein Ssb-like OB fold" evidence="2">
        <location>
            <begin position="19"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="153"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="174"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF21473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9683644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="205" mass="22480" checksum="65A022C4710C8706" modified="2024-01-24" version="1">MAESKSGLRKPVFTKIEQLRPGTSGHTLTVKAVSSKMVLQKGRPDGPQVRQMRIAGCLVGDETGMIIFTARNRIKKLSLACLLTEKAVCGNPSESTIKSDGAKEKMAKGNKSTLGRTKRKIVPAVLETMESQKDVDGEEAQTDENVMKVEKENRVPDSTIETSTNTKHVMKLKNSIEIEKENKPIGDGDKNTSQRKQQTRKSAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z873</accession>
    <name>A0AA38Z873_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dirigent protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_016551</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Secreted</location>
            <location evidence="4">Extracellular space</location>
            <location evidence="4">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the plant dirigent protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z873"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009699">
        <property type="term" value="P:phenylpropanoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.480.10">
        <property type="entry name" value="Allene oxide cyclase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044859">
        <property type="entry name" value="Allene_oxi_cyc_Dirigent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004265">
        <property type="entry name" value="Dirigent"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46215:SF5">
        <property type="entry name" value="DIRIGENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46215">
        <property type="entry name" value="DIRIGENT PROTEIN 24-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03018">
        <property type="entry name" value="Dirigent"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0052">Apoplast</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0964">Secreted</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9684109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="365" mass="36596" checksum="059A6372F1D1F189" modified="2024-01-24" version="1">MAVQKLVSFTAKAIAYLLLLTVTIAFSTSARVLEEQPELPISLSGASNSVQQPVAGVAPVATPAVAQPYPGVAAAATPAVAQPNPGVPAAATPAVAQANPGAAAQANPGAAAQANPGAVAQPNPGAVAATSAGMAHDDHPLSFFMHDILGGSNPSARAVTGIVTNPAVNGQVPFAKPNGALLPVNNGVPLNSGNSGLINNNNIPFLTGLSGITPTVIPNNGNNLFNGGNGLPVGNGGQYLAGSSLQKLMFGTMTVIDDELTDGHELGSGSVGKAQGFYVASSEDGTSQTMAFTAMFQSGSYADSLSFFGVHRTAVSESQLAIMGGTGKYVNAKGFATVKTIPPTHQQAATDGVETLLEFTVYVTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z881</accession>
    <name>A0AA38Z881_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WRKY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_016509</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z881"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.80">
        <property type="entry name" value="WRKY domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003657">
        <property type="entry name" value="WRKY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036576">
        <property type="entry name" value="WRKY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044810">
        <property type="entry name" value="WRKY_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31282">
        <property type="entry name" value="WRKY TRANSCRIPTION FACTOR 21-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03106">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00774">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118290">
        <property type="entry name" value="WRKY DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50811">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="WRKY" evidence="6">
        <location>
            <begin position="105"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9684050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="313" mass="34949" checksum="12047DB6C5EA9DB3" modified="2024-01-24" version="1">MGTSPPERLSTDQKRVVGELVHGQDLANQLQILLREPFSDLRSVSAEDLVVKILRSFTEALAVLRCYDQSGEAGGGSPAESGNCKVLKNRRGCYKRRKNSETWTAVSSTIEDGHAWRKYGQKEILNAKFPRSYYRCTRKHEQGCRATKQVQRMKENPILYHTTYIGHHTCRDILKAPQFIGGSYPGYDSNNMVGSESKIPEEVQEMKPELMKEETVVSDLTADNVSSFDSINLWSGLEALDSSVPAMVTPRGGSDTHGNQDQDVDSTMYSRNATTTTTATTASHNTDMDFVLGCLDFEGGDQFQFDESPFQFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z895</accession>
    <name>A0AA38Z895_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_016386</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small hydrophilic plant seed protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9683862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z895"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009737">
        <property type="term" value="P:response to abscisic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038956">
        <property type="entry name" value="LEA_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022377">
        <property type="entry name" value="Sm_Hydphi_plant_seed_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000389">
        <property type="entry name" value="Small_hydrophilic_seed_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34671">
        <property type="entry name" value="EM-LIKE PROTEIN GEA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34671:SF11">
        <property type="entry name" value="EM-LIKE PROTEIN GEA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00477">
        <property type="entry name" value="LEA_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00431">
        <property type="entry name" value="SMALL_HYDR_PLANT_SEED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="33"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9683862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="93" mass="9944" checksum="D2D0542213D08069" modified="2024-01-24" version="1">MASRQENRAELDAKARQGETVVPGGTGGGSLEAQEHLAEGRSRGGQARKEQIGREGYQEMGRKGGLSTVDQSGGERAEQEGIPIDESKFRTKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z8A9</accession>
    <name>A0AA38Z8A9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DC1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_016643</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z8A9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46477">
        <property type="entry name" value="CYSTEINE/HISTIDINE-RICH C1 DOMAIN FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46477:SF15">
        <property type="entry name" value="CYSTEINE_HISTIDINE-RICH C1 DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="230"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9684256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="257" mass="29691" checksum="20B8B9392C19DD19" modified="2024-01-24" version="1">MELYPRQKVINSHPHSPLILKPAQKPYHCGGCKEYEIDLRQCYQCEHKNVHQKCDFHLHVECDPSSKSIWLQFINCTFTFQQEAPRGKKMVCDGCGKDVKGWFYQCSSRGNPRYLHPCCAKLPFKRTDKGGMVLDLKEKTSSVCLICGNEGNSKDFRSWVYVSRCGKYCYHVACVKDKVERWNKEEASNQQQSYRAGTSSGALVRRAPNQEIKTGRREKVLDYLEMAFDIFTFVVLLIFGVSVPVPYGLVIKWLKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z8Y8</accession>
    <name>A0AA38Z8Y8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein ENHANCED DISEASE RESISTANCE 2-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_016872</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z8Y8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00821">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00177">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.530.20:FF:000068">
        <property type="entry name" value="Pleckstrin homology (PH) and lipid-binding START domains-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045096">
        <property type="entry name" value="EDR2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009769">
        <property type="entry name" value="EDR2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002913">
        <property type="entry name" value="START_lipid-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12136">
        <property type="entry name" value="ENHANCED DISEASE RESISTANCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12136:SF103">
        <property type="entry name" value="PROTEIN ENHANCED DISEASE RESISTANCE 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07059">
        <property type="entry name" value="EDR2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01852">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00234">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50848">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="3">
        <location>
            <begin position="7"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="START" evidence="4">
        <location>
            <begin position="152"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9684618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="703" mass="80261" checksum="55A1F48E471F04E5" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZAY1</accession>
    <name>A0AA38ZAY1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_017213</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
            <location evidence="1">Centromere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZAY1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042054">
        <property type="term" value="F:histone methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.280.10:FF:000003">
        <property type="entry name" value="Histone-lysine N-methyltransferase, H3 lysine-9 specific SUVH5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.280.10">
        <property type="entry name" value="SRA-YDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025794">
        <property type="entry name" value="H3-K9-MeTrfase_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051357">
        <property type="entry name" value="H3K9_HMTase_SUVAR3-9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003616">
        <property type="entry name" value="Post-SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007728">
        <property type="entry name" value="Pre-SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036987">
        <property type="entry name" value="SRA-YDG_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003105">
        <property type="entry name" value="SRA_YDG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45660">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SETMAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45660:SF73">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE, H3 LYSINE-9 SPECIFIC SUVH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05033">
        <property type="entry name" value="Pre-SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02182">
        <property type="entry name" value="SAD_SRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00468">
        <property type="entry name" value="PreSET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00466">
        <property type="entry name" value="SRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50868">
        <property type="entry name" value="POST_SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50867">
        <property type="entry name" value="PRE_SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51575">
        <property type="entry name" value="SAM_MT43_SUVAR39_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51015">
        <property type="entry name" value="YDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0137">Centromere</keyword>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="2" id="KW-0158">Chromosome</keyword>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="YDG" evidence="14">
        <location>
            <begin position="248"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="Pre-SET" evidence="12">
        <location>
            <begin position="471"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="domain" description="SET" evidence="11">
        <location>
            <begin position="535"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="domain" description="Post-SET" evidence="13">
        <location>
            <begin position="700"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="130"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9685094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="716" mass="79483" checksum="618960B0A2D7C005" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZFV7</accession>
    <name>A0AA38ZFV7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Dolichyl-diphosphooligosaccharide-protein glycosyltransferase subunit OST5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_014065</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the oligosaccharyltransferase (OST) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the OST5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZFV7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008250">
        <property type="term" value="C:oligosaccharyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007915">
        <property type="entry name" value="TMEM258/Ost5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13636">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 258"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05251">
        <property type="entry name" value="Ost5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="80"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="124"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9688142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="143" mass="15410" checksum="D2E5BB521F3A584B" modified="2024-01-24" version="1">MKFVSTHQTALVDLTSPVSTAQDSSTCEIWCSVEALKPRISGRKLRSRVFSQIETVVLFSSPQRAMAPAAKPITSPVPDAWYPTLAVLMLAVGLVITASFFIYEATSSRKSRSLAKELTTGAMASIFLGFGSLFLLLSSGVYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZHC5</accession>
    <name>A0AA38ZHC5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Receptor-like serine/threonine-protein kinase</fullName>
            <ecNumber evidence="19">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">PVL29_014357</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="24">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="19"/>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZHC5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00028">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01098">
        <property type="entry name" value="PAN_AP_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000248">
        <property type="entry name" value="S-receptor-like kinase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.90.10.10:FF:000008">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000178">
        <property type="entry name" value="serine/threonine-protein kinase PBS1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.10">
        <property type="entry name" value="Bulb-type lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.4.10">
        <property type="entry name" value="Hepatocyte Growth Factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051343">
        <property type="entry name" value="G-type_lectin_kinases/EP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024171">
        <property type="entry name" value="SRK-like_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47976">
        <property type="entry name" value="G-TYPE LECTIN S-RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD2-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47976:SF1">
        <property type="entry name" value="G-TYPE LECTIN S-RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE SD2-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000641">
        <property type="entry name" value="SRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 19 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 19" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0430">Lectin</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 19 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 19" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="5 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Bulb-type lectin" evidence="22">
        <location>
            <begin position="26"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="23">
        <location>
            <begin position="316"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="490"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="518"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRNR" id="PIRNR000641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KAJ9688662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="813" mass="90632" checksum="9615F9ADCBB7B588" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZJM3</accession>
    <name>A0AA38ZJM3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_012349</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9689608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZJM3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19446">
        <property type="entry name" value="REVERSE TRANSCRIPTASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="254"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9689608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="360" mass="41557" checksum="E8F6D5498B2BA663" modified="2024-01-24" version="1">MWLKEEGFKDKIKKWWGSLKFTGTSSYILDAKLRALKNILKIWNKEEFGLIETKKGDALRQVEFWDEKEKHAALNLEECEARNEARESYKSWVMREEISWRQKSRELWLKEGDNNTRYFHRMANAHGRRNWLSKLKVDGCWHLEENNLKNSVVGAFQKLYSEDEGWRPSIDGLSFSELGSSEAEGLEIPFSEEEVFAALSDLGKDKALGPDGFTMAFWLFCWDVVKVEIMGFVRDFYDRGRFVKSLNATFLVLVPKKGDAEDLKDFRPISLVGSLYKLLAKVLANRIKKVMGKVISEPQNAFVEGRQILDAVLIANEAVDSRLKSNQGGVMCKLDIEKAYDHVCWNFLLAVLKKMGFGER</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZJP7</accession>
    <name>A0AA38ZJP7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RIN4 pathogenic type III effector avirulence factor Avr cleavage site domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_012496</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9689862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZJP7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040387">
        <property type="entry name" value="RIN4/NOI4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008700">
        <property type="entry name" value="TypeIII_avirulence_cleave"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33159:SF49">
        <property type="entry name" value="RPM1-INTERACTING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33159">
        <property type="entry name" value="RPM1-INTERACTING PROTEIN 4 (RIN4) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05627">
        <property type="entry name" value="AvrRpt-cleavage"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RIN4 pathogenic type III effector avirulence factor Avr cleavage site" evidence="2">
        <location>
            <begin position="6"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="RIN4 pathogenic type III effector avirulence factor Avr cleavage site" evidence="2">
        <location>
            <begin position="164"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="129"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Cys residues" evidence="1">
        <location>
            <begin position="228"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9689862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="237" mass="26005" checksum="4C266943E4A75802" modified="2024-01-24" version="1">MALVAARSHVPKFGNWDNDNVPYTAYFDNARKEKTGPTGLRINPNDPEENPEAFIFGRSEATPPHHLNPQKSPPEENNQVEGRRRLNSHRRSPSDHQQRSGGTSRSINSESGSEKSNLDYSHLRKKSQLSDGGSSSLSPSVPGPSHTRLKSIGTPPSDDHAHRVASVPKFGAWDETDPTSGEGFTVIFNKVKQERQGAATTLPRVPPPQSAYSNSQKKHQNSSSRSKICCCFSPGRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZKP6</accession>
    <name>A0AA38ZKP6_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_012606</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZKP6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35493">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35493:SF1">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="126"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="385"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="169"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="132"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="394"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9690029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="404" mass="45091" checksum="AB577E1BCCCF797B" modified="2024-01-24" version="1">MAPIKSSSRYDSFDARSSASSQFSDPSSSVELNKNQRFVAPSKSHPPESSSRALALARTKSSDLAPAKVKNEQNLSAMVKKFMEKRSSKPKAASTNRNGLFVPADFIAEDLKKTARKGTSFSGLHRKLFTKGSGSSGSSGSSRGEKSEVKALTEVKANTRTLAMVLRSERELLSQNKEQEMEIAELKLVIEEKNREVEKLKDLCLKQREEIKSLKSAILFPDVMNSHLQDLLDKQGSELKQAKQLIPTLQKQVTSLTGQLQCLAEDLAEVKADKYSVRACFQGHCSSPRTPTYDQDEAANSLDFSSEDPATPGSPDDMFLKDLNPCLTPYYAKTKSKEFEVDYDSPPHERLYENNLQFDSRVRKMSRSSEYSQNSNVASIAIRAARRSDQSKHTYGKPMHHKLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZM13</accession>
    <name>A0AA38ZM13_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_013122</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZM13"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000109">
        <property type="entry name" value="POT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11654">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00854">
        <property type="entry name" value="PTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="115"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="157"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="307"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="349"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="390"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="442"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="475"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="522"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9690808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="568" mass="62819" checksum="E84F730C8F08C80A" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZNF1</accession>
    <name>A0AA38ZNF1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Inositol oxygenase</fullName>
            <ecNumber evidence="4 12">1.13.99.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Myo-inositol oxygenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_011312</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>myo-inositol + O2 = D-glucuronate + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23696"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
            <dbReference type="ChEBI" id="CHEBI:58720"/>
            <dbReference type="EC" id="1.13.99.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11 12">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="11 12">Binds 2 iron ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Polyol metabolism; myo-inositol degradation into D-glucuronate; D-glucuronate from myo-inositol: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the myo-inositol oxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.13.99.1" evidence="4 12"/>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZNF1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050113">
        <property type="term" value="F:inositol oxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019310">
        <property type="term" value="P:inositol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019853">
        <property type="term" value="P:L-ascorbic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007828">
        <property type="entry name" value="Inositol_oxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12588:SF12">
        <property type="entry name" value="INOSITOL OXYGENASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12588">
        <property type="entry name" value="MYOINOSITOL OXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05153">
        <property type="entry name" value="MIOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0060">Ascorbate biosynthesis</keyword>
    <keyword evidence="5 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 11 12" id="KW-0408">Iron</keyword>
    <keyword evidence="7 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="10">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="102"/>
            <end position="104"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="161"/>
            <end position="162"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="239"/>
            <end position="240"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR607828-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR607828-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU367039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9692177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="304" mass="35808" checksum="50BDFAF0EFD9488E" modified="2024-01-24" version="1">MTIIIDQPDFVEVVEKKVDAEQENELVLDGGFVVPESNSFGHTFRDYDAESDRKDGVENFYRINHINQTYDFVKKMREEYSRLNRVQMSIWECCELLNDVVDESDPDLDEPQIEHLLQTAEAIRKDYPNEDWMHLTALIHDLGKVLLLPSFGELPQWAVVGDTFPVGCAFDESIVHHKYFKENPDYYNPAYNTKYGVYSEGCGLENVMMSWGHDDYMYLVAKENKTTLPSAGLFVIRYHSFYALHRSDAYKYLMNEEDIENLKWLKIFNKYDLYSKSKVRIDVEKVKPYYLSLIEKYFPAKLNW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZNK7</accession>
    <name>A0AA38ZNK7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MAR-binding filament-like protein 1-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_011458</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZNK7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000793">
        <property type="term" value="C:condensed chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000796">
        <property type="term" value="C:condensin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43941:SF5">
        <property type="entry name" value="ELKS_RAB6-INTERACTING_CAST FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43941">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="653"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="129"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="653"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="677"/>
            <end position="690"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9692406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="690" mass="78145" checksum="1322608C1AC23920" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZPF3</accession>
    <name>A0AA38ZPF3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GBF-interacting protein 1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_011194</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9691978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZPF3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009719">
        <property type="entry name" value="GIP1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47070">
        <property type="entry name" value="HYDROXYPROLINE-RICH GLYCOPROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47070:SF2">
        <property type="entry name" value="OS06G0206100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06972">
        <property type="entry name" value="GIP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GBF-interacting protein 1 N-terminal" evidence="2">
        <location>
            <begin position="15"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="280"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="457"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="534"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="144"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="290"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="344"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="398"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="457"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="482"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="506"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="535"/>
            <end position="547"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9691978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="861" mass="92326" checksum="64285709631E4105" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZPN0</accession>
    <name>A0AA38ZPN0_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_011631</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZPN0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9692645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="114" mass="12387" checksum="E94CFB4F1D9AF7E0" modified="2024-01-24" version="1">MGSESIANAYKRRRRDEEGRSDTEEDINGGSESLTVSEEEDNEEGTDNESARGYASESIIEQSEDAETEDNDETSDTDQMHGLDVDDQSAVGASENTPQPAPLEGCRKASNTTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZPS4</accession>
    <name>A0AA38ZPS4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_011931</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9693016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZPS4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009791">
        <property type="term" value="P:post-embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000233">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase TDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000213">
        <property type="entry name" value="Tyrosine-sulfated glycopeptide receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051502">
        <property type="entry name" value="RLP_Defense_Trigger"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48062">
        <property type="entry name" value="RECEPTOR-LIKE PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48062:SF52">
        <property type="entry name" value="RECEPTOR-LIKE PROTEIN 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="11">
        <location>
            <begin position="3"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9693016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="731" mass="83201" checksum="1D2312A3A7DC507E" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZR23</accession>
    <name>A0AA38ZR23_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ELMO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_009533</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9693617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZR23"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006816">
        <property type="entry name" value="ELMO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050868">
        <property type="entry name" value="ELMO_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12771:SF56">
        <property type="entry name" value="CED-12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12771">
        <property type="entry name" value="ENGULFMENT AND CELL MOTILITY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04727">
        <property type="entry name" value="ELMO_CED12"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51335">
        <property type="entry name" value="ELMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ELMO" evidence="2">
        <location>
            <begin position="140"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9693617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="280" mass="32688" checksum="C7F51A51C3104458" modified="2024-01-24" version="1">MTSKTLRRRLHHGDVDGKRHEHLETSGLDGLNEPLLGDYEYDDKDSEGPVLKDLWDDERRKEHQHWMILFSHLIAEWAQWLANIVIGSGTLLKRLLPFDSTTQNGRSKKILPPSLSPLQEERLRNMQQRLEVPFDGSCVEHQDALRELWSLAYPGRELPCLKSELWKEMGWQGTDPSTDFRGGGFISLENLIFFAKKYPESQLLRQAVRFLQLLGEDEMAFDNLFCVAFQMMDAQWLAKRASYMEFNDVLKSTRTQLERELELEDVFSVRDLPAYNMLRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZRG9</accession>
    <name>A0AA38ZRG9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_011956</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9693044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZRG9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000383">
        <property type="entry name" value="Leucine-rich repeat receptor protein kinase EMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000041">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000111">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051502">
        <property type="entry name" value="RLP_Defense_Trigger"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48062">
        <property type="entry name" value="RECEPTOR-LIKE PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48062:SF52">
        <property type="entry name" value="RECEPTOR-LIKE PROTEIN 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 14" id="KW-0732">Signal</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041269141" description="Leucine-rich repeat-containing N-terminal plant-type domain-containing protein" evidence="14">
        <location>
            <begin position="29"/>
            <end position="1106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1047"/>
            <end position="1070"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="15">
        <location>
            <begin position="33"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9693044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1106" mass="125279" checksum="66A5585653275494" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZRN9</accession>
    <name>A0AA38ZRN9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_009456</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9693507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZRN9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039537">
        <property type="entry name" value="Retrotran_Ty1/copia-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057670">
        <property type="entry name" value="SH3_retrovirus"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648:SF31">
        <property type="entry name" value="RNA-DIRECTED DNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648">
        <property type="entry name" value="TRANSPOSASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25597">
        <property type="entry name" value="SH3_retrovirus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="2">
        <location>
            <begin position="35"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="301"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="305"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9693507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="459" mass="52051" checksum="E6F4775155856030" modified="2024-01-24" version="1">MFPHLFQGLDISEFHGETCELAKHTHVSFPISNKRSYHPFHLIHSDIWGPSTIPNISGACWFVSLIDDCTWVTWIFLLKQKSDVSIVIPNFHSMVQNQFGVKIKSFRTDNARDYFNQTLSPDFRSQGILHDSSCVNTPQQNGVAERKNGHLLNTTRALLFQENAPNSYWGEVVLTATYMINKIPSRVLDNKSPVEILKSFYPHFRTSNGLTPRVFGCIAFVHVHSQHKDKLDPQAIKCVFLGYSSTQKGYKCYNPSARKFYISADVTFTENKPFFPRSSLQGEISMMKYSPYESFESLNLPHVSTHGDEEPKSSEPESSDSITPESPKFTTEPVSSPIPASVTRNFPQVPKVYSREKVIPKQKQVQESNSDLGNEITVRLYPPLYTQPGETSTDSTDNLDLDLPIVVRKGTRECTNRPLYPLSHYVSFKHLSPAHKNFIVSLNTTIIPNTISEALTKRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZSU2</accession>
    <name>A0AA38ZSU2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DNA topoisomerase 6 subunit A</fullName>
            <ecNumber evidence="12">5.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">TOP6A</name>
        <name evidence="18" type="ORF">PVL29_010219</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Component of the DNA topoisomerase VI involved in chromatin organization and progression of endoreduplication cycles. Relaxes both positive and negative superturns and exhibits a strong decatenase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 20">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="12">Homodimer. Heterotetramer of two TOP6A and two TOP6B subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 12 13">Belongs to the TOP6A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="12"/>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZSU2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009330">
        <property type="term" value="C:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000228">
        <property type="term" value="C:nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042138">
        <property type="term" value="P:meiotic DNA double-strand break formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000706">
        <property type="term" value="P:meiotic DNA double-strand break processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00223">
        <property type="entry name" value="TOPRIM_TopoIIB_SPO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000387">
        <property type="entry name" value="DNA topoisomerase 6 subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1360.10:FF:000003">
        <property type="entry name" value="DNA topoisomerase 6 subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1360.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00132">
        <property type="entry name" value="Top6A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002815">
        <property type="entry name" value="Spo11/TopoVI_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013049">
        <property type="entry name" value="Spo11/TopoVI_A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036078">
        <property type="entry name" value="Spo11/TopoVI_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049333">
        <property type="entry name" value="Topo_VI_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004085">
        <property type="entry name" value="TopoVI_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034136">
        <property type="entry name" value="TOPRIM_Topo6A/Spo11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10848:SF4">
        <property type="entry name" value="DNA TOPOISOMERASE 6 SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10848">
        <property type="entry name" value="MEIOTIC RECOMBINATION PROTEIN SPO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21180">
        <property type="entry name" value="TOP6A-Spo11_Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20768">
        <property type="entry name" value="Topo_VI_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04406">
        <property type="entry name" value="TP6A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01550">
        <property type="entry name" value="TOP6AFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01552">
        <property type="entry name" value="TPISMRASE6A"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56726">
        <property type="entry name" value="DNA topoisomerase IV, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52041">
        <property type="entry name" value="TOPO_IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10 12 13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12 13" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="Spo11/DNA topoisomerase VI subunit A N-terminal" evidence="15">
        <location>
            <begin position="134"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Type II DNA topoisomerase VI subunit A all-beta" evidence="16">
        <location>
            <begin position="199"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="Topoisomerase 6 subunit A/Spo11 TOPRIM" evidence="17">
        <location>
            <begin position="244"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="12">
        <location>
            <position position="163"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-tyrosine intermediate" evidence="13">
        <location>
            <position position="163"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="301"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF04406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF20768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF21180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ9694641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="420" mass="46864" checksum="E6289D35816BD31D" modified="2024-01-24" version="1">MAEKKKRRRTDPSDSDQQHLPFKNSLKSDEIILQTLKDLCSSSSSDGAAGTLTLSDLALASTCREVADLSLSSVQSAIETLILRLTHSILSGQGFSFSVPSRSSANQLYVPELDRIVLKDKSSVRPFANVSTVRKSAVTTRILQLIHQLCIKNIHVTKRDLFYTDVKLFQDQTQSDSVLDDVSCMLGCTRSSLNVIASEKGVVVGRLIFSDNGDMIDCTKMGMGGKAIPPNIDRVGDMQSDALFILLVEKDAAYIRLAEDRFYNRFPCIIVTAKGQPDVATRLFLRKMKTELKLPVLALVDSDPYGLKILSVYGCGSKNMSYDSANLTTPDIKWLGIRPSDLDKYNIPEQCRLPMTEQDIKTGKDLLEEDFVKKNPGWVEELNLMVKTKQKAEIQALSSFGFQYLSEVYLPLKLQQQDWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZU15</accession>
    <name>A0AA38ZU15_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycosyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_010669</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="5"/>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZU15"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035251">
        <property type="term" value="F:UDP-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000047">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000071">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058980">
        <property type="entry name" value="Glyco_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48047">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48047:SF237">
        <property type="entry name" value="UDP-GLYCOSYLTRANSFERASE 73C3-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26168">
        <property type="entry name" value="Glyco_transf_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="3 4" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041301119" description="Glycosyltransferase" evidence="6">
        <location>
            <begin position="22"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase N-terminal" evidence="7">
        <location>
            <begin position="10"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF26168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9695295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="495" mass="55048" checksum="94F73ABEC9581150" modified="2024-01-24" version="1">MASQLLQLHFVLIPFMAPGHLIPMVDMARLLAQHGVIVTVVTTPLNAARFKSMIDRAVESGLQIRLLELQFPAVEAGLPEGCENVDLLPSRSLIRNFFVAASMLQQPLEQLFQELQPRPSCIISGKNLAWTADTARKFQIPRLYFDSMSCFAFSCSHNLEASKVHESISKLETFLVPGLPDQIEFTKAQLPESLNPGSSDLTGILNQMRASESIADGIVVNTYEELEPRYVKEYKRIKGDKVWCIGPVSACNKLNLDKAERGKKALVDENQCLRWLDSWEPNSVVYACLGSISGLTALQLIELGFGLEASNRPFIWVIRGGEKSNELERWILEEGFEERTEGRGLLIRGWAPQMLILSHPSIGVFLTHCGWNSTLEGVCTGVPILTCPLFAEQFINEKLVVQILGIGVSVGVESAVTWGMEEKFGVVMKREDVMKAIDEVMDKGEGGEKRRKRARELGEMAKKAIEEGGSSYLNMKILIHYILQQTIGNPSTQLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZWF3</accession>
    <name>A0AA38ZWF3_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_008363</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZWF3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9696072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="126" mass="13811" checksum="45747A9ED37161C3" modified="2024-01-24" version="1">MAAMDIKRDDNPNNSFLGKSLPKSFSWCLDVRMARNMTTAVTEKHRAMRDAIEKTLKAGWFSRVGTPSPSLTPPGTVADVANATVAMRAATTEHELEVLLSHSPPFSTRSAWLFVNISLAVLLLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZWK1</accession>
    <name>A0AA38ZWK1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Large ribosomal subunit protein uL15/eL18 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_008656</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZWK1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.100.10.10:FF:000001">
        <property type="entry name" value="60S ribosomal protein L18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.100.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000039">
        <property type="entry name" value="Ribosomal_eL18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021131">
        <property type="entry name" value="Ribosomal_uL15/eL18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036227">
        <property type="entry name" value="Ribosomal_uL15/eL18_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10934">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10934:SF25">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL18X-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17135">
        <property type="entry name" value="Ribosomal_L18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52080">
        <property type="entry name" value="Ribosomal proteins L15p and L18e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL15/eL18" evidence="5">
        <location>
            <begin position="2"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="152"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="160"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="177"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF17135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9696541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="187" mass="21029" checksum="91C52CCE3B047783" modified="2024-01-24" version="1">MGIDLVAGGKSKRTKRTAPKSDDIYLKLLVKLYRFLVRRTGSKFNAVILKRLFMSKVNKPPLSLSRLVRFMDGKEGKIAVVVGTVTDDLRVYEVPSLKVTALRFTERARARIEKAGGECLTFDQLALRAPLGQNTVLLRGPKNAREAVKHFGKAPGVPHSHTKPYVRSKGRKFERARGRRNSRGFRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZYH0</accession>
    <name>A0AA38ZYH0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_006984</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9697641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZYH0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.150.80:FF:000004">
        <property type="entry name" value="NAC transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.80">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003441">
        <property type="entry name" value="NAC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036093">
        <property type="entry name" value="NAC_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31719:SF216">
        <property type="entry name" value="NAC DOMAIN-CONTAINING PROTEIN 68-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31719">
        <property type="entry name" value="NAC TRANSCRIPTION FACTOR 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02365">
        <property type="entry name" value="NAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101941">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51005">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NAC" evidence="7">
        <location>
            <begin position="86"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="154"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="258"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="261"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9697641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="375" mass="42935" checksum="49EE8956F61E5326" modified="2024-01-24" version="1">MMDSTLDGRFALVKSVQLTAAKYLPQGTTPTHSSFAYHNPHQQRCPTSAPPPPNFSGSIIHYSTSARHTYNQAMMRERDAEAELNLPPGFRFHPTDEELVVHYLCRKASNQTLPVPIIVEIDLYKYDPWQLPEKALFGKKEWYFFTPRDRKYPNGSRPNRAAGHGYWKATGADKPIRPTGSTKTVGIKKALVFYSGKAPKGTKTDWIMHEYRLAQPNRSSDKKISSRLDDWVLCRIYNKKNNWRERMQHQMNQLLPHEPIDPFEEAEDDGSDSLRTPESDIDIETLSDNHDLHGAQGQGYSYQVLHDNNLQITDTDSGFPAQECFKEEDDYWFTNLNLEDLQNSLATFGTMPLPDFSYQDSFSMIHGLPYQPAAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZYQ5</accession>
    <name>A0AA38ZYQ5_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_009181</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZYQ5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9697272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="75" mass="8748" checksum="9AA7C3A6864677F6" modified="2024-01-24" version="1">MSFLQFLFPVLHSSRHLFDYQRNCKILLGLLPTSSISPRLHNCIVRTPKMTLIWMRRNDNIILLVGPFPLTLLKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A040</accession>
    <name>A0AA39A040_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Disease resistance RPP13-like protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_009357</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A040"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056789">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766:SF51">
        <property type="entry name" value="DISEASE RESISTANCE RPP13-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766">
        <property type="entry name" value="PLANT BROAD-SPECTRUM MILDEW RESISTANCE PROTEIN RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25019">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0611">Plant defense</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="7">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="6">
        <location>
            <begin position="143"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="8">
        <location>
            <begin position="399"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="domain" description="R13L1/DRL21-like LRR repeat region" evidence="9">
        <location>
            <begin position="656"/>
            <end position="783"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF25019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9697498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1257" mass="142913" checksum="484F2C632B4A6491" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A156</accession>
    <name>A0AA39A156_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_007826</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A156"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04623">
        <property type="entry name" value="CBS_pair_bac_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.580.10:FF:000017">
        <property type="entry name" value="CBS domain-containing protein CBSX3, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044725">
        <property type="entry name" value="CBSX3_CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051257">
        <property type="entry name" value="Diverse_CBS-Domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080:SF2">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0129">CBS domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="67"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="135"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9698942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="205" mass="22715" checksum="8C3F626439A82CBF" modified="2024-01-24" version="1">MQGAMQAIVSHGSILRNAVLHRIRLVNPIMPSVVFSRLQSVTSARMEEHGFESTTIADVLKAKGKSADGSWLWCTTDDSVYDAVKSMTQHNVGALVVVKPGEQKSIAGIITERDYLRKIIVQGRSSKSTKVGDIMTEENKLITVSPNTKVLRAMQLMTDNRIRHIPVINDKEMIGMVSIGDVVRAVVTEHREELDRLNAYIQGGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A2D9</accession>
    <name>A0AA39A2D9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Bet v I/Major latex protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_005520</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BetVI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9699690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A2D9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010427">
        <property type="term" value="F:abscisic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009738">
        <property type="term" value="P:abscisic acid-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07816">
        <property type="entry name" value="Bet_v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.530.20:FF:000007">
        <property type="entry name" value="Major pollen allergen Bet v 1-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000916">
        <property type="entry name" value="Bet_v_I/MLP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024949">
        <property type="entry name" value="Bet_v_I_allergen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050279">
        <property type="entry name" value="Plant_def-hormone_signal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31213">
        <property type="entry name" value="OS08G0374000 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31213:SF55">
        <property type="entry name" value="STRESS-INDUCED PROTEIN SAM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00407">
        <property type="entry name" value="Bet_v_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00634">
        <property type="entry name" value="BETALLERGEN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0568">Pathogenesis-related protein</keyword>
    <keyword evidence="2" id="KW-0611">Plant defense</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bet v I/Major latex protein" evidence="4">
        <location>
            <begin position="1"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9699690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="158" mass="17208" checksum="52591C2C4DE689FB" modified="2024-01-24" version="1">MGVFTYEQEITSSVPPAKMFKAAVLDADNLIPKVRPQAIKCVEIIQGEGGPGTIRKIHFGEGSKFKSMTHRVDAIDKEKFSFCYTVIDGDVLTGSIESISHELTVVPSPAGGSIYKNTTKYHTKGDLVCEEHVKGGKEDSLATFKAIEAYVQAHPDAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A3M0</accession>
    <name>A0AA39A3M0_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_005918</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A3M0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009543">
        <property type="term" value="C:chloroplast thylakoid lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009523">
        <property type="term" value="C:photosystem II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010207">
        <property type="term" value="P:photosystem II assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010206">
        <property type="term" value="P:photosystem II repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025585">
        <property type="entry name" value="PSII_Psb27"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34041:SF3">
        <property type="entry name" value="PHOTOSYSTEM II D1 PRECURSOR PROCESSING PROTEIN PSB27-H2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34041">
        <property type="entry name" value="PHOTOSYSTEM II REPAIR PROTEIN PSB27-H1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13326">
        <property type="entry name" value="PSII_Pbs27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9700356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="139" mass="15412" checksum="ED85493186CCB315" modified="2024-01-24" version="1">MRMAVFLAMKTCPPMIPKPIEKNAYINVNKLKSRCHIVLPSQESPSSRRHVLVGSGASLVAILTFNGGFSPLPVWAEDESNGQEDDEKKIKSGKVLPKAYLNSAREVVKTLRERTADAAKESIREYLSSWRGKQTVVGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A3Z8</accession>
    <name>A0AA39A3Z8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Receptor-like serine/threonine-protein kinase</fullName>
            <ecNumber evidence="18">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">PVL29_005905</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="24">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="18"/>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A3Z8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048544">
        <property type="term" value="P:recognition of pollen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000237">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000059">
        <property type="entry name" value="S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.90.10.10:FF:000006">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.10">
        <property type="entry name" value="Bulb-type lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051343">
        <property type="entry name" value="G-type_lectin_kinases/EP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000858">
        <property type="entry name" value="S_locus_glycoprot_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024171">
        <property type="entry name" value="SRK-like_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47976">
        <property type="entry name" value="G-TYPE LECTIN S-RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD2-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47976:SF64">
        <property type="entry name" value="RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00954">
        <property type="entry name" value="S_locus_glycop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000641">
        <property type="entry name" value="SRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 18 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0430">Lectin</keyword>
    <keyword evidence="12 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 18 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 18" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 21" id="KW-0732">Signal</keyword>
    <keyword evidence="4 18" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041305754" description="Receptor-like serine/threonine-protein kinase" evidence="21">
        <location>
            <begin position="22"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="429"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="23">
        <location>
            <begin position="26"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="488"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="520"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRNR" id="PIRNR000641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KAJ9700339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="779" mass="87442" checksum="A2C2CB3752A7B28C" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A447</accession>
    <name>A0AA39A447_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_008252</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9699568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A447"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043424">
        <property type="entry name" value="BLT-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31071">
        <property type="entry name" value="GB|AAF24581.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31071:SF16">
        <property type="entry name" value="MYB-LIKE PROTEIN Z ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="98"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="413"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="448"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="541"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="227"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="98"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="116"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="419"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="450"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="551"/>
            <end position="561"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9699568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="648" mass="73042" checksum="A60D88340660BA0B" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A4B7</accession>
    <name>A0AA39A4B7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_006172</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A4B7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="25"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="132"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9700725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="245" mass="27651" checksum="DD13ABDEEF9B36FA" modified="2024-01-24" version="1">MSGNGWRLPSQSYQVQQIEYFPFICCWCHHSFMDMEALIDHHEAHLMLLQNQVETYYPTTFFRPQRGGTFSMNPFRPLLPLPDTTAMIRAPTSNNSVGVHSSQFSLPCHQNVAGSSATQSHLPLLRGINRSRQLPMHSPPRVERQRGTNKGPLNATSRQHVMRPPQQMARQGLIEGRPSKAARLQHEIEPPPSVEELQGQTKGTSNVVTKPLINCFGRPEPVATDQHEEGNETLDLTLSLRTPYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A4U4</accession>
    <name>A0AA39A4U4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA-directed RNA polymerase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_006047</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">DNA-dependent RNA polymerase which catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A4U4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.120">
        <property type="entry name" value="RNA polymerase Rpb7-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036898">
        <property type="entry name" value="RNA_pol_Rpb7-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045113">
        <property type="entry name" value="Rpb7-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12709">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II, III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12709:SF3">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE V SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88798">
        <property type="entry name" value="N-terminal, heterodimerisation domain of RBP7 (RpoE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0804">Transcription</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU369086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9700550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="164" mass="18707" checksum="83F1FD1D2EE71F7D" modified="2024-01-24" version="1">MFLKVQLPWNVIIPAECLDAEGLMLQRSIIIRLLDAFSNKKATQELGYFLAVTTSENIGEGKVRQHSGDVLFAVVFSCVTFMLFRGEILDGVVHKVLKHGVILRCGPVENICLSCQKMPDYRYVPGENPVFLNEKLSKIEKDVVVRFIVWGQSRLRLNGNFRHW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A597</accession>
    <name>A0AA39A597_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_006163</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A597"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9700707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="56" mass="6241" checksum="30349B21AA9AC908" modified="2024-01-24" version="1">MLLLRLLVVVLTAMVLMLMLMLVLSALEVSISGTESMDKCLIFGRAQAWRRGVSPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A5Y5</accession>
    <name>A0AA39A5Y5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_006814</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A5Y5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.370:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 17.6-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000026">
        <property type="entry name" value="Transposon Ty3-G Gag-Pol polyprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005162">
        <property type="entry name" value="Retrotrans_gag_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041577">
        <property type="entry name" value="RT_RNaseH_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13975">
        <property type="entry name" value="gag-asp_proteas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03732">
        <property type="entry name" value="Retrotrans_gag"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17919">
        <property type="entry name" value="RT_RNaseH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0229">DNA integration</keyword>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="10">
        <location>
            <begin position="654"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="313"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="334"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9701595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1137" mass="129944" checksum="138F01C07C320FE3" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A677</accession>
    <name>A0AA39A677_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">chitinase</fullName>
            <ecNumber evidence="2">3.2.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_006851</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1-&gt;4)-beta-linkages in chitin and chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.14"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.14" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A677"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008843">
        <property type="term" value="F:endochitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016998">
        <property type="term" value="P:cell wall macromolecule catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00325">
        <property type="entry name" value="chitinase_GH19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00035">
        <property type="entry name" value="ChtBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.20.10:FF:000001">
        <property type="entry name" value="Endochitinase (Chitinase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.530.10:FF:000052">
        <property type="entry name" value="Endochitinase PR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.530.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.20.10">
        <property type="entry name" value="Endochitinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.10">
        <property type="entry name" value="Endochitinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001002">
        <property type="entry name" value="Chitin-bd_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018371">
        <property type="entry name" value="Chitin-binding_1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036861">
        <property type="entry name" value="Endochitinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016283">
        <property type="entry name" value="Glyco_hydro_19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000726">
        <property type="entry name" value="Glyco_hydro_19_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22595:SF194">
        <property type="entry name" value="CHITINASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22595">
        <property type="entry name" value="CHITINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00187">
        <property type="entry name" value="Chitin_bind_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00182">
        <property type="entry name" value="Glyco_hydro_19"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001060">
        <property type="entry name" value="Endochitinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00270">
        <property type="entry name" value="ChtBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="Lysozyme-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57016">
        <property type="entry name" value="Plant lectins/antimicrobial peptides"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00026">
        <property type="entry name" value="CHIT_BIND_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50941">
        <property type="entry name" value="CHIT_BIND_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00773">
        <property type="entry name" value="CHITINASE_19_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00774">
        <property type="entry name" value="CHITINASE_19_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7" id="KW-0146">Chitin degradation</keyword>
    <keyword evidence="3 14" id="KW-0147">Chitin-binding</keyword>
    <keyword evidence="8 13 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0611">Plant defense</keyword>
    <keyword evidence="11" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 15" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041278349" description="chitinase" evidence="15">
        <location>
            <begin position="25"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-1" evidence="16">
        <location>
            <begin position="16"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="12">
        <location>
            <position position="136"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13 14">
        <location>
            <begin position="29"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13 14">
        <location>
            <begin position="34"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="92"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="154"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="239"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR001060-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR001060-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9701657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="271" mass="29158" checksum="95B9327EF4125F70" modified="2024-01-24" version="1">MVAKLVAILIVGVLAGALPWPVLAQNYSCNAGLCCSQFGFCGTGVRYCGDGCQAGPCYSPPSGGGDGGCISVADVVTEDFFDGIINQVDASCAGKNFYTRRAFLDALNSYPEFGQGGYANDSKREIAAFFAHATHETGCFCYIEEIDGPSKNYCVEDTRYPCVPGKGYYGRGPLQITRNYNYGPAGESIGFNGLNNPEIVANDPVISFKTAFWFWMKTVHDIVNRGFSATIHAINGSECNGGNRDAVNARVEYYKDYCNQLGVFPGDNLPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ABY8</accession>
    <name>A0AA39ABY8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dirigent protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_002793</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Secreted</location>
            <location evidence="4">Extracellular space</location>
            <location evidence="4">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the plant dirigent protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ABY8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009699">
        <property type="term" value="P:phenylpropanoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.480.10">
        <property type="entry name" value="Allene oxide cyclase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044859">
        <property type="entry name" value="Allene_oxi_cyc_Dirigent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004265">
        <property type="entry name" value="Dirigent"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21495">
        <property type="entry name" value="NUCLEOPORIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03018">
        <property type="entry name" value="Dirigent"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0052">Apoplast</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0964">Secreted</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9704389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="166" mass="18634" checksum="F40EA766C9DE1E22" modified="2024-01-24" version="1">MAMAMPRVHSTIGDPEEADEWLHKIQHAKQKVTRLHFYFHDTVSGQNVSAMRVTQASITDKSPSFFGLINMIDDPLTEGPELTSKQVGRAQGLYGLSGLNDMSLLMVMNFAFTSGEYNGSTLSILGMKEHCNAANPRDARSWWEWGFPTGSWDCNSRDVFPQFHYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AF79</accession>
    <name>A0AA39AF79_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">TMPIT-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_001782</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM120 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AF79"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012926">
        <property type="entry name" value="TMEM120A/B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21433:SF0">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 120 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21433">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN INDUCED BY TUMOR NECROSIS FACTOR ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07851">
        <property type="entry name" value="TMEM120A-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="141"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="171"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="201"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="232"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="272"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="317"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9706442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="361" mass="41099" checksum="75A1137FE1E6A55B" modified="2024-01-24" version="1">MGDSATLEQSAGDIDEQVSGVVEQARELQDAASSHISRNSVEEQALRQRALSLDSNIHRLRSLIDSLHRNKILDPKLADKLDEDLQRARCILVDGDASSFLPGNAQGGFLRMFLGPINVRATRKDVQLKVKEEYNSYRDRTALLFLLFPSTLLILRSWIWDGCLPTFPVQLYQAWLLFLYTGLALRENILRVNGSDIRPWWIYHHYCAMIMALVSLTWEIKGQPNCTQKQRGVELFLQWAMMQGVAMLLQNRYQRQRLYTRIALGKAKRMDVVWGETAGVHGQLWLLCPILFILQGFEAYVGLLLLKTALVGVVSEWQVVFCGVLLVLMAVGNFANTVQTLMAKSRFKAKMKRSKSKQELN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AJ29</accession>
    <name>A0AA39AJ29_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">H15 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_000448</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AJ29"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031492">
        <property type="term" value="F:nucleosomal DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030261">
        <property type="term" value="P:chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045910">
        <property type="term" value="P:negative regulation of DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00073">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000493">
        <property type="entry name" value="HMG-Y-related protein A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017956">
        <property type="entry name" value="AT_hook_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005818">
        <property type="entry name" value="Histone_H1/H5_H15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11467:SF109">
        <property type="entry name" value="H15 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11467">
        <property type="entry name" value="HISTONE H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00538">
        <property type="entry name" value="Linker_histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00929">
        <property type="entry name" value="ATHOOK"/>
    </dbReference>
    <dbReference type="SMART" id="SM00526">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51504">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="H15" evidence="6">
        <location>
            <begin position="65"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="128"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="245"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="141"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="152"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="277"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9708406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="388" mass="44126" checksum="5F69B8098805A34F" modified="2024-01-24" version="1">MDSQPQNPNPHTTSPNHKQKRNMDKLKKAVLGAMTLDSATPLSEETKKFIEKRLLQLFPVIRTPDHPPYAWMILDAIKTLKEKRGSSEKSLSEFIKSNYEVPWAHSSYLSHHLCKLAQNGDIVVTSDDHYMIPTGNPNPNPKRKEKRKKRKCQEGRGRRGNYDKEAVVEKKNQLGEQEKVEIKELSQAQKHENYVIDGANEQDYQVNEGKNQSQGQQNEVRSELLQSSCLNDDNCATLMVIPVESSSPRVEDEEGESVEELPKQQNGRKSPFDSLPPVNTQHHFEKQQPQSQHQGIASIPEEDTDTGALLPIGPPQHTLSQHQGQGRPPKPQPDTITRDEALLPLQHESYQQHPPQNRGRGRPRKPKPDVEGFLTSQNLRRGRPRKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AJ84</accession>
    <name>A0AA39AJ84_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_000233</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AJ84"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9708072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="104" mass="12022" checksum="7613F62FCC226CC1" modified="2024-01-24" version="1">MKIAIAALDVLHSYPATPRMKNLVPYCRLSFYMEGDNSLAFSGCIDSIQYCHFQVISGIVSMATCTKRFCNKIMYYHVIRFCKGTKRFCTLETFPIMYYLPHVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AJJ7</accession>
    <name>A0AA39AJJ7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Ubiquinol oxidase</fullName>
            <ecNumber evidence="17">1.10.3.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PVL29_002580</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 17">
            <text>2 a ubiquinol + O2 = 2 a ubiquinone + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:30255"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="EC" id="1.10.3.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="17">Binds 2 iron ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer; disulfide-linked.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the alternative oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.10.3.11" evidence="17"/>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AJJ7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098803">
        <property type="term" value="C:respiratory chain complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009916">
        <property type="term" value="F:alternative oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106292">
        <property type="term" value="F:superoxide-generating NADPH oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102721">
        <property type="term" value="F:ubiquinol:oxygen oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010230">
        <property type="term" value="P:alternative respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01053">
        <property type="entry name" value="AOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1260.140:FF:000001">
        <property type="entry name" value="Ubiquinol oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.140">
        <property type="entry name" value="Alternative oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002680">
        <property type="entry name" value="AOX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038659">
        <property type="entry name" value="AOX_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31803">
        <property type="entry name" value="ALTERNATIVE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31803:SF3">
        <property type="entry name" value="ALTERNATIVE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01786">
        <property type="entry name" value="AOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 17" id="KW-0249">Electron transport</keyword>
    <keyword evidence="14 17" id="KW-0408">Iron</keyword>
    <keyword evidence="16 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13 17" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 17" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="7 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="41"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ9707608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="322" mass="36379" checksum="1B8CB0D48A86D1F6" modified="2024-01-24" version="1">MMMSRSAMKLLSTATVRTIHGGGEFRQGFFALGARNLSSTVSAVREKPQTEPGSGSGSAAGHRSFEGGDKSVVSYWGVAPVKLTKEDGTEWKWSCFKPSETYKADVSIDLGKHHVPGVWTDKVAYWGVKMLRLPTDIFFKRRFEMRAMMLETVAAVPGMVGGMLLHCKSLRRFEHSGGWIRALLEEAENERMHLMTFMEVAKPRWYERALVFAVQGIFWNLYFVAYVISPKVAHRVVGYLEEEAVHSYNEFIKEIDSGNIPNVPAPAIAIDYWRLPPDSTLRDVVMVVRADEAHHRDVNHYASDIHYQGLQLKEFPAPLGYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AK33</accession>
    <name>A0AA39AK33_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Germin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_000872</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Secreted</location>
            <location evidence="1 11">Extracellular space</location>
            <location evidence="1 11">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the germin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AK33"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02241">
        <property type="entry name" value="cupin_OxOx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006045">
        <property type="entry name" value="Cupin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001929">
        <property type="entry name" value="Germin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019780">
        <property type="entry name" value="Germin_Mn-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31238">
        <property type="entry name" value="GERMIN-LIKE PROTEIN SUBFAMILY 3 MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00190">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00325">
        <property type="entry name" value="GERMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00835">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00725">
        <property type="entry name" value="GERMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-0052">Apoplast</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 9 11" id="KW-0464">Manganese</keyword>
    <keyword evidence="5 9 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Cupin type-1" evidence="12">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU366015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9709113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="124" mass="13264" checksum="71E6EBF4FAFE375F" modified="2024-01-24" version="1">MARADIAANGLVPLHSHPRASEVSILLQGALLVGFIDTSNRLFTQLLRPGDSFVFPKGMIHFLYNLDSLAPALVLAGLNSQNPGTQLTSMAAFATNPLLPDEVLKKAFQISGQDVAKIRRKLGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AKA9</accession>
    <name>A0AA39AKA9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">aspartate-semialdehyde dehydrogenase</fullName>
            <ecNumber evidence="5">1.2.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_000537</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-aspartate 4-semialdehyde + phosphate + NADP(+) = 4-phospho-L-aspartate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24284"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57535"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="1.2.1.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 2/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the aspartate-semialdehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.11" evidence="5"/>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AKA9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004073">
        <property type="term" value="F:aspartate-semialdehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019877">
        <property type="term" value="P:diaminopimelate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18131">
        <property type="entry name" value="ASADH_C_bac_euk_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02316">
        <property type="entry name" value="VcASADH2_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02121">
        <property type="entry name" value="ASADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012080">
        <property type="entry name" value="Asp_semialdehyde_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005986">
        <property type="entry name" value="Asp_semialdehyde_DH_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000534">
        <property type="entry name" value="Semialdehyde_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012280">
        <property type="entry name" value="Semialdhyde_DH_dimer_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01296">
        <property type="entry name" value="asd_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011456">
        <property type="entry name" value="PRK14874.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46278:SF2">
        <property type="entry name" value="ASPARTATE-SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46278">
        <property type="entry name" value="DEHYDROGENASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01118">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02774">
        <property type="entry name" value="Semialdhyde_dhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000148">
        <property type="entry name" value="ASA_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00859">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0220">Diaminopimelate biosynthesis</keyword>
    <keyword evidence="11" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="12" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="8" id="KW-0521">NADP</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0791">Threonine biosynthesis</keyword>
    <feature type="domain" description="Semialdehyde dehydrogenase NAD-binding" evidence="15">
        <location>
            <begin position="44"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="14">
        <location>
            <position position="174"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000148-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9708545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="379" mass="41377" checksum="1BBB6F6BEF29A768" modified="2024-01-24" version="1">MKAPALAHHHHLLTATRIPRTNLRPRIATPTSTIRMSLQENAPSLAVVGVTGAVGQEFLSVLSDRGFPYRSIKMLASKRSAGKHLTFEDNDYVIEELTADSFDGIDIALFSAGGSISKQFGPIAVDRGSIVVDNSSAFRMDENVPLIIPEVNPDAMKHIKLGSGKGALIANPNCSTIICLMAATPLHRSAKVVRMVVSTYQAASGAGAAAMEELELQTREVLEGKQPTCKIFKQQYAFNLFSHNAPVLPNGYNEEEMKLVKETRKIWNDMNVKVTATCIRVPVMRAHAESVNLQFENPLDEDKARDILKNAPGVVVIDERPSNHFPTPLEVSNKDDVAVGRIRRDVSQDGNFGLDIFVCGDQIRKGAALNAIQIAEMLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ALP6</accession>
    <name>A0AA39ALP6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 22">Peroxidase</fullName>
            <ecNumber evidence="5 22">1.11.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">PVL29_001121</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="24">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 22">
            <text>2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:56136"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:139520"/>
            <dbReference type="ChEBI" id="CHEBI:139521"/>
            <dbReference type="EC" id="1.11.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="19 22">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="19 22">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="19 22">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="19 22">Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 22">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peroxidase family. Ascorbate peroxidase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="22">Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.7" evidence="5 22"/>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ALP6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140825">
        <property type="term" value="F:lactoperoxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00693">
        <property type="entry name" value="secretory_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.420.10:FF:000013">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.520.10:FF:000006">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.420.10">
        <property type="entry name" value="Peroxidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000823">
        <property type="entry name" value="Peroxidase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019793">
        <property type="entry name" value="Peroxidases_heam-ligand_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033905">
        <property type="entry name" value="Secretory_peroxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31235">
        <property type="entry name" value="PEROXIDASE 25-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00458">
        <property type="entry name" value="PEROXIDASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00461">
        <property type="entry name" value="PLPEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00435">
        <property type="entry name" value="PEROXIDASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50873">
        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 19 22" id="KW-0106">Calcium</keyword>
    <keyword evidence="14 21" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 22" id="KW-0349">Heme</keyword>
    <keyword evidence="16 22" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="13 19 22" id="KW-0408">Iron</keyword>
    <keyword evidence="9 19 22" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 22" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7 22" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 22" id="KW-0964">Secreted</keyword>
    <keyword evidence="10 22" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041481454" description="Peroxidase" evidence="22">
        <location>
            <begin position="28"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="Plant heme peroxidase family profile" evidence="23">
        <location>
            <begin position="47"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="17">
        <location>
            <position position="88"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="19">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="300"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="20">
        <location>
            <position position="84"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="57"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="90"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="145"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="224"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU362060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KAJ9709489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="375" mass="40983" checksum="38AF88776B305F31" modified="2024-01-24" version="1">MQSSTFRLAFLVVLISVLLSLRDPSKGEPQNHAIHGISPPKLDLQPSLEYDFYRNSCPKAESIVRSSMAQIFAAHSDAPPALLRLLFHDCFIQGCDASILLDDSNESMNRSAEKLAIPNQTLKGFDKVGKIKEELEKACPGVVSCADILVLATRDGIVLAGGPFYPVLTGRRDSNQSYFQEAMDDIPKPDGNITQTLGLFTLRGFNERETVSLLGGHSIGKISCEFIQGRLFNFSGTGQPDPSIASDFLDEMRRNCQDSGNSSHGMASPPMVSRAMSELTLGMTYYQGLSSSVSSGSAFDTHYYQSLLQGRGLLFSDQQLMAEEKTERLVRAYASDDGSTFQMDFARSMMKMSSLSVLTGSQGQVRLNCSKMVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D4G9</accession>
    <name>A0AA39D4G9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_025381</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D4G9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021319">
        <property type="entry name" value="DUF2921"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057425">
        <property type="entry name" value="DUF2921_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33389">
        <property type="entry name" value="FAMILY PROTEIN, PUTATIVE (DUF2921)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33389:SF18">
        <property type="entry name" value="OS01G0677900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11145">
        <property type="entry name" value="DUF2921"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25333">
        <property type="entry name" value="DUF2921_N"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="628"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="659"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="716"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="793"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2921" evidence="12">
        <location>
            <begin position="47"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2921" evidence="12">
        <location>
            <begin position="352"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2921" evidence="12">
        <location>
            <begin position="422"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="domain" description="SWEET-like" evidence="11">
        <location>
            <begin position="617"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="domain" description="SWEET-like" evidence="11">
        <location>
            <begin position="717"/>
            <end position="829"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF11145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF25333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9671648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="848" mass="94304" checksum="49568C630D5DE5EE" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D6P8</accession>
    <name>A0AA39D6P8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_027288</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9671227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D6P8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000257">
        <property type="entry name" value="Zinc-finger homeodomain protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006455">
        <property type="entry name" value="Homeodomain_ZF_HD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01565">
        <property type="entry name" value="homeo_ZF_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31948:SF174">
        <property type="entry name" value="HOMEOBOX DOMAIN, ZF-HD CLASS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31948">
        <property type="entry name" value="ZINC-FINGER HOMEODOMAIN PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="129"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="10">
        <location>
            <begin position="131"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9671227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="197" mass="22154" checksum="AD490E231962E9D1" modified="2024-01-24" version="1">MSISRQQVEVQVPKTSSTHTRGNGHDDESPSPSPPFKEGMHQAYKKAATEGEFSYTFGHLQPLNTEGKLILGHHNKPIMGPKSTEYPAGTLVSSRAAAPQHMIMGSIPSESDEQEEIGRGGAKPSSVQQVKKRFRTKFTQEQKEKMLSFAERAGWRIQKQEESLVQQLCQEIGIKRRVFKVWMHNNKNLARKNPSTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D8S4</accession>
    <name>A0AA39D8S4_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_024863</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9676073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D8S4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080030">
        <property type="term" value="F:methyl indole-3-acetate esterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080032">
        <property type="term" value="F:methyl jasmonate esterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080031">
        <property type="term" value="F:methyl salicylate esterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009694">
        <property type="term" value="P:jasmonic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009696">
        <property type="term" value="P:salicylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045889">
        <property type="entry name" value="MES/HNL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10992:SF785">
        <property type="entry name" value="METHYLESTERASE 14, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10992">
        <property type="entry name" value="METHYLESTERASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9676073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="170" mass="19137" checksum="40BFFCF9BB12ECD7" modified="2024-01-24" version="1">MGNKFICMTKKDGRENGSKSKRVGRSQRKMLAEEEFLHRQALSMAIQQHQLSQRFDGSMSRRIGSTSSRRHTLSDPFSNGKQEPDILENVKTKKFVLVHGEGFGAWSWYKTIALLEEVGLAPTALDLRGSGIDRTDTNSVTTLADYSKPLTDYLENLPEDEKVRGCVKSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D8Y3</accession>
    <name>A0AA39D8Y3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Valencene synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_024988</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9676253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D8Y3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010333">
        <property type="term" value="F:terpene synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016102">
        <property type="term" value="P:diterpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00684">
        <property type="entry name" value="Terpene_cyclase_plant_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034741">
        <property type="entry name" value="Terpene_cyclase-like_1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044814">
        <property type="entry name" value="Terpene_cyclase_plant_C1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001906">
        <property type="entry name" value="Terpene_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050148">
        <property type="entry name" value="Terpene_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005630">
        <property type="entry name" value="Terpene_synthase_metal-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225">
        <property type="entry name" value="OS04G0344100 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225:SF241">
        <property type="entry name" value="TERPENE SYNTHASE FAMILY, METAL-BINDING DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01397">
        <property type="entry name" value="Terpene_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03936">
        <property type="entry name" value="Terpene_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01019">
        <property type="entry name" value="Terpene_Cyclase_Like_1_C_Termi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Terpene synthase N-terminal" evidence="3">
        <location>
            <begin position="28"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="Terpene synthase metal-binding" evidence="4">
        <location>
            <begin position="221"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9676253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="473" mass="54743" checksum="38393DEC4A868C7F" modified="2024-01-24" version="1">MSIQVSACPLVQIPKPENRPRAEFHPSIWGDQCIAYTPDDEDTRACKEKQVEDLKEEVRRELMVAAGNPSQLLNFIDAMQRLGVAYHFEREIEESLQHIYDRFHDVDDTDDDLYNIALQFRLLRQQGYNISCGIFNKFKDEKGSFKEDLISNIQGMLGLYEAAHLRVHGEDILEEALAFTTTHLKATVESLGYPLAEQVAHALKHPIRKGLERLEARWWWKELDFATKLPFARDRFVEGYFWILGVYFEPQYSCARRILTKLFSMASIIDDIYDAYGTLEELQPFTEAIERWDINSIDHLPEYMKLFYVTLLDMYKEIDQELEKDGNQYRVYYAKEVLKSQVRAYFAEAKWSHEGYIPTIEEYMLVALITSGSCMLATWSFIGMGEIMTKEGFDWVISDPKIITASTVIFRLMDDITTHKECLKPTVVPMPLLTPVVNLSRVMDVIYKEGDGYTHVGKVMKDNIGSVLIDPIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D9V3</accession>
    <name>A0AA39D9V3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DYW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_025047</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. PCMP-H subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9676331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D9V3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:002156">
        <property type="entry name" value="Putative pentatricopeptide repeat-containing protein At1g56570"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:003648">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032867">
        <property type="entry name" value="DYW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF382">
        <property type="entry name" value="PENTACOTRIPEPTIDE-REPEAT REGION OF PRORP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14432">
        <property type="entry name" value="DYW_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="54"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="257"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="289"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="DYW" evidence="4">
        <location>
            <begin position="469"/>
            <end position="564"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9676331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="564" mass="62663" checksum="9CD3C61E13C14EF7" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DE79</accession>
    <name>A0AA39DE79_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FLZ-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_019720</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FLZ family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DE79"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044533">
        <property type="entry name" value="FLZ1/2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007650">
        <property type="entry name" value="Zf-FLZ_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46057">
        <property type="entry name" value="FCS-LIKE ZINC FINGER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46057:SF13">
        <property type="entry name" value="FLZ-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04570">
        <property type="entry name" value="zf-FLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51795">
        <property type="entry name" value="ZF_FLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FLZ-type" evidence="6">
        <location>
            <begin position="31"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="FLZ-type" evidence="4">
        <location>
            <begin position="31"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="82"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9680470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="105" mass="11736" checksum="3F22729E5F9B2924" modified="2024-01-24" version="1">MAASSSSSSSSSSRSGLLYYAGCEEYPHQPHFLEACFLCHKPLGNNTDIFMYRGNTPFCSKECRQEQMEFDEAKEKSWNMMSLRKSDSNKSSSKKTVRTGTVAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DEH0</accession>
    <name>A0AA39DEH0_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_020002</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DEH0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11073">
        <property type="entry name" value="CYP76-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000007">
        <property type="entry name" value="Cytochrome P450 76C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47950">
        <property type="entry name" value="CYTOCHROME P450, FAMILY 76, SUBFAMILY C, POLYPEPTIDE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47950:SF44">
        <property type="entry name" value="CYTOCHROME P450, FAMILY 76, SUBFAMILY C, POLYPEPTIDE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0349">Heme</keyword>
    <keyword evidence="4 5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="5">
        <location>
            <position position="442"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9680859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="499" mass="56283" checksum="58DEDBCBA9385114" modified="2024-01-24" version="1">MDYTPLVLLLLLPCFVWLCFHFLILGSTHWKSFQARLPPGPRPLPIIGNLLELGDKPHQSLTTLSRAYGPLMSLKLGSTTTIVISSSETAQEVLNKKDQAFASRTLLNAIQAQDHHKFSIVFLPASAHWRNLRKICSMQIFSPQRVEASQDLRRKVVQQLLEHARESCNSGRAVDVGRAAFTTTLNLLSNTFFSVDLAHYDSNLSQEFKDLIWSIMVEAGKPNLADFFPGLRLVDPQGIQKRMAVYFNKLADVFDRFINQRLPLKASSPDNDVLDALLNLNKQHDHELSCNDIRHLLLDLFSAGTDTTSSTVEWAMAELLNNPKAMAKAQDELSQVVGKDRIVEESDITKLPYLQAVVKETFRLHPPVPFLVPRKAEMDSEILGYAVPKNAQVLVNVWAIGRDSRTWSNPNSFVPERFLECEIDVKGRDFQLIPFGAGRRICPGLLLGHRMVHLVLASLLHSFDWKLEDGMKPEDMDMSEKFGFTLRKAQPLRAVPTKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DF74</accession>
    <name>A0AA39DF74_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Neprosin PEP catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_020374</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9681475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DF74"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1320.10">
        <property type="entry name" value="Outer-capsid protein sigma 3, large lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053168">
        <property type="entry name" value="Glutamic_endopeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004314">
        <property type="entry name" value="Neprosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025521">
        <property type="entry name" value="Neprosin_propep"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31589:SF221">
        <property type="entry name" value="LIGASE, PUTATIVE (DUF239)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31589">
        <property type="entry name" value="PROTEIN, PUTATIVE (DUF239)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03080">
        <property type="entry name" value="Neprosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14365">
        <property type="entry name" value="Neprosin_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52045">
        <property type="entry name" value="NEPROSIN_PEP_CD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041318481" description="Neprosin PEP catalytic domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="Neprosin PEP catalytic" evidence="2">
        <location>
            <begin position="142"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS52045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9681475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="390" mass="43056" checksum="7C4C77840D222DC8" modified="2024-01-24" version="1">MGIKEIIGCYSVLFLLIIVDADGVWSISKEEDMELEKQLKILNKPAVKTIRTDSDQIFDCVDIHKQPSLDHPLLKNHKVRTTFDDFSETMKDRPLKGKGLSGFGVRCPIGTVPIRRVEKEDLIRAKAFSKLHMKTYAKNSHPLADAPIHLSAKLAANDKSVYGVAGILNVYQPTLLSPDQVSGSIIYLSTGFGEPFKSVISVGWAVMPPVYRDNFTHLFAYWTADNGKTSGCYDILCPGFVIQSPEFALGRKFPGSTYRGSQVDFNVRIFKDTKSGDWYLWVNGVSVGYWPKEIFNGVFEFAKEVNWGGEIFSPRQPCPPMGSGHFDGERIGGACYISGMQYQIAPAGKFVDIDPALKAVVDSPQCYKASMPSNEGTGYTFLFGGPGGQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DFY7</accession>
    <name>A0AA39DFY7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_018443</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DFY7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057135">
        <property type="entry name" value="At4g27190-like_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050905">
        <property type="entry name" value="Plant_NBS-LRR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463:SF198">
        <property type="entry name" value="RPP4C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23247">
        <property type="entry name" value="LRR_RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0611">Plant defense</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="172"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9682524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="898" mass="101538" checksum="3F4CF973BA94D45B" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DHG4</accession>
    <name>A0AA39DHG4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Angiotensin-converting enzyme 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_016555</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DHG4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006476">
        <property type="entry name" value="CHP01589_pln"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01589">
        <property type="entry name" value="A_thal_3526"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31871">
        <property type="entry name" value="OS02G0137100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31871:SF61">
        <property type="entry name" value="OS06G0705300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09713">
        <property type="entry name" value="A_thal_3526"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9684114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="93" mass="10747" checksum="D88FB5163558F15A" modified="2024-01-24" version="1">MGDSSASYIHMVQHLIEKCLIFHMSKEECMEALSKHADINQVITSTVWNELEKENKEFFEAYHTQTQSGDDRMSEAETNEMIQKMIADSKDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DMK0</accession>
    <name>A0AA39DMK0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">SRP54-type proteins GTP-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_014585</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GTP-binding SRP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9689005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DMK0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005785">
        <property type="term" value="C:signal recognition particle receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005047">
        <property type="term" value="F:signal recognition particle binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14826">
        <property type="entry name" value="SR_alpha_SRX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17876">
        <property type="entry name" value="SRalpha_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.140:FF:000007">
        <property type="entry name" value="Signal recognition particle receptor subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000016">
        <property type="entry name" value="Signal recognition particle receptor subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000188">
        <property type="entry name" value="signal recognition particle receptor subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.140">
        <property type="entry name" value="Signal recognition particle SRP54, nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007222">
        <property type="entry name" value="Sig_recog_particle_rcpt_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013822">
        <property type="entry name" value="Signal_recog_particl_SRP54_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036225">
        <property type="entry name" value="SRP/SRP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042101">
        <property type="entry name" value="SRP54_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43134">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE RECEPTOR SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43134:SF1">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE RECEPTOR SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04086">
        <property type="entry name" value="SRP-alpha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02881">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00963">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47364">
        <property type="entry name" value="Domain of the SRP/SRP receptor G-proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00300">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SRP54-type proteins GTP-binding" evidence="9">
        <location>
            <begin position="589"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="126"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="218"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="279"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="137"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="160"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="290"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9689005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="616" mass="67712" checksum="FBAF1850435916A1" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DQA0</accession>
    <name>A0AA39DQA0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_011328</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DQA0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000394">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000146">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase IOS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024788">
        <property type="entry name" value="Malectin-like_Carb-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45631">
        <property type="entry name" value="OS07G0107800 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45631:SF206">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12819">
        <property type="entry name" value="Malectin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="502"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="530"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9692199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="810" mass="90778" checksum="F4EBBF07A9C5955B" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DRQ8</accession>
    <name>A0AA39DRQ8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein ALUMINUM SENSITIVE 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_010194</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0014 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DRQ8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010044">
        <property type="term" value="P:response to aluminum ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005226">
        <property type="entry name" value="UPF0014_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30028:SF0">
        <property type="entry name" value="PROTEIN ALUMINUM SENSITIVE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30028">
        <property type="entry name" value="UPF0014 INNER MEMBRANE PROTEIN YBBM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03649">
        <property type="entry name" value="UPF0014"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="69"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="98"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="127"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9694598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="264" mass="28662" checksum="3FE4EC93A2EDD383" modified="2024-01-24" version="1">MDFDWVMDFLRGMVKPVAALAVVLMAVALSYFQKLGLEGEMVYSIFRAFLQLSVIGFVLQFIFTQANSGWIVLAYFFMVSVAGYTAGQRAKHVPRGKYVAGASILAGTAVTMFLLLVLNVFPFTPRYIIPVAGMMVGNAMTVTGVTMKRLRDDIKIQLNLVETALALGATPRQATLQQVKRALVIALSPVLDNAKTVGLISLPGAMTGLIMGGASPIEAIQLQIVVMNMLVGASTVSSIMSTYLCWPAFFTKAHQLEIHVFRSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DS00</accession>
    <name>A0AA39DS00_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-CH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_010365</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DS00"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46214:SF36">
        <property type="entry name" value="RING-CH-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46214">
        <property type="entry name" value="ZINC FINGER, RING-CH-TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="212"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="RING-CH-type" evidence="6">
        <location>
            <begin position="97"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="167"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="178"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9694853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="236" mass="25355" checksum="2341395B7FB266F1" modified="2024-01-24" version="1">MSGVEMSHIDLEQGGHRRSFGGSNGSGDGSLCFSDADEGSCYSLFYSTAGGSYDEYSFACVTDAEVGGVSDSRRVSSMSDCSVEVETENGVPEIKVHLAKAERDCRICHLSLESSNYESGIPIELGCSCKDDLAAAHKHCAEAWFKIKGNKTCEICNSTARNVFSASEMEVTEHSNETSNNSTAPAPAPAPAAVSTTVSGAETRSFWHGHRFLNFLLACMVFAFVISWLFHFNVPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DT33</accession>
    <name>A0AA39DT33_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein OSB2, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_008315</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9695993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DT33"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006264">
        <property type="term" value="P:mitochondrial DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04496">
        <property type="entry name" value="SSB_OBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000314">
        <property type="entry name" value="Protein OSB2 chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000424">
        <property type="entry name" value="Primosome_PriB/ssb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011344">
        <property type="entry name" value="ssDNA-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00621">
        <property type="entry name" value="ssb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10302">
        <property type="entry name" value="SINGLE-STRANDED DNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10302:SF0">
        <property type="entry name" value="SINGLE-STRANDED DNA-BINDING PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00436">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50935">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9695993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="286" mass="32516" checksum="EEE2C99B510079A2" modified="2024-01-24" version="1">MLNQTVGRTSILDCVPKIHLFKTQTLSFSPCPNPFFFSLPQPKTQLRCSIICNYNNDHHNQAVSYPRPSEVPWKKELCNSVSLIGIVGAPVEIKHLSSGKVLAWTRLAVRKSASDTTWINLTFWDELAHVASQHVEKGQQIYVSGRLVSDTVENDEGKQQTYYKVVVQQLNFVERSFSSADESNSLAAGRKLGSPATNSTGSIEELWQAFFANPVDWWDNRKTKKNPKYPDFKHKDTGEALWVEGRYNPSWVKSQLAILDSRMESFRDQDARMHVNLMAGDDFMPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DV27</accession>
    <name>A0AA39DV27_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_009130</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DV27"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14798">
        <property type="entry name" value="RX-CC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038005">
        <property type="entry name" value="RX-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF1052">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RPM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NB-ARC" evidence="4">
        <location>
            <begin position="148"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="5">
        <location>
            <begin position="407"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="6">
        <location>
            <begin position="523"/>
            <end position="773"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9697209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="777" mass="88042" checksum="D5A23468498047A6" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DVR7</accession>
    <name>A0AA39DVR7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein FLC EXPRESSOR</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_007702</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FLX family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DVR7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009908">
        <property type="term" value="P:flower development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040353">
        <property type="entry name" value="FLX/FLX-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33405:SF17">
        <property type="entry name" value="PROTEIN FLC EXPRESSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33405">
        <property type="entry name" value="PROTEIN FLX-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0221">Differentiation</keyword>
    <keyword evidence="5" id="KW-0287">Flowering</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="77"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="220"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9698768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="314" mass="34455" checksum="0C9465CB62772DAE" modified="2024-01-24" version="1">MAGRNHLPPPNALKIREVSSTRAPYPSHHNHHALIEEDRYRHRSGPIPVSAGRYHPAVIDERIVVQHREIQTLLGDNQRLAATHVALKQELAAAQQEISHLSAAAASVKAERDAQVREVYERSLKMEAEVRSIDALNAELAQVRADVQKLSASRQELAAQLQTINTDLAASRSELKEVQAVKDGIENMRKELHRGRAAIEYEKKTHANNLEQSEAMGKNMMAMAHEVEKLRAELANAEKRARAAAAAAAAANPSPVVHAYAASYGNSDMGYGGSFYHNPYAMHQAHGDPDAGPQYGAGAVPLDPYNMQRAHAHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DVY5</accession>
    <name>A0AA39DVY5_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_007839</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DVY5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030132">
        <property type="term" value="C:clathrin coat of coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030130">
        <property type="term" value="C:clathrin coat of trans-Golgi network vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.110">
        <property type="entry name" value="Clathrin heavy-chain terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016025">
        <property type="entry name" value="Clathrin_H-chain_N"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50989">
        <property type="entry name" value="Clathrin heavy-chain terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9698963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="73" mass="7813" checksum="7B11D94E62D2781D" modified="2024-01-24" version="1">MNMSGSSMAATNTPITMKEAPTLPSLGVSRLCMSRWNLPLRRPITADATLMNPNTRILALKAQLSGTTADLNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E0A2</accession>
    <name>A0AA39E0A2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ammonium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_004316</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ammonia transporter channel (TC 1.A.11.2) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E0A2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008519">
        <property type="term" value="F:ammonium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3430.10:FF:000005">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3430.10">
        <property type="entry name" value="Ammonium transporter AmtB like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029020">
        <property type="entry name" value="Ammonium/urea_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001905">
        <property type="entry name" value="Ammonium_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018047">
        <property type="entry name" value="Ammonium_transpt_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024041">
        <property type="entry name" value="NH4_transpt_AmtB-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002229">
        <property type="entry name" value="RhesusRHD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00836">
        <property type="entry name" value="amt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43029:SF7">
        <property type="entry name" value="AMMONIUM TRANSPORTER 2 MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43029">
        <property type="entry name" value="AMMONIUM TRANSPORTER MEP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00909">
        <property type="entry name" value="Ammonium_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00342">
        <property type="entry name" value="RHESUSRHD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111352">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01219">
        <property type="entry name" value="AMMONIUM_TRANSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0924">Ammonia transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="26"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="55"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="118"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="145"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="183"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="213"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="276"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="300"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="333"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="362"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="393"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="Ammonium transporter AmtB-like" evidence="9">
        <location>
            <begin position="26"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9702519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="482" mass="52376" checksum="A827B4A077CF68E3" modified="2024-01-24" version="1">MADIPFPSNLLPDEASPEWMNKGDNAWQLTAATLVGLQTVPGLIILYGGAVKKKWAVNSAFMAFYAFACVLICWVGWGYRMSFGTKLVPFWGTANVALDQAYLLDQAFVGKFPEATMVFFQFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWCPSGFLAKAGIIDYSGGYVIHLSSGVAGFTAAYWVGPRLTKDRERFPPNNILLMMAGAGLLWMGWTGFNGGDPYVVSIDASLAVLNTHVCTATSLLTWLILDIAVFGKASIIGAVQGMITGLVCITPAAGVVQGWAAIIMGIFSGSIPWFTMMVVHKKSELLQKVDDTMAVLHTHAIAGCLGGIMTGLFASPKLNRLFYGYGVDVDSTYVGLFYGFHMGEVRKGFRQVGIQLLGIVFVLVFNVIATSLICLVVQLIVPLRMSDEDMEIGDEAAHGEEAYAIWGQGDKLENSRYGSSVYGDLEVPMPKPRGAGQVEMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E1K0</accession>
    <name>A0AA39E1K0_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_005198</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E1K0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33872">
        <property type="entry name" value="DNA POLYMERASE EPSILON CATALYTIC SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33872:SF7">
        <property type="entry name" value="OSJNBA0084K11.10-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9703824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="202" mass="23260" checksum="7AAF11CEEDBDCB1A" modified="2024-01-24" version="1">MDLPLHGFNFSVFLSSFCDPRLLLVANPTSDQVFEFKGKEKQMELKAELGPAKMKQVSPPPPTLPPPLPRFWVATKPMITESVTNQEIAKFWRQKRMEEEDHLLPALKAAARIRAQKLTEDEYQRYEESLEDIDNEDSSKKAENDKALRVGIKDWWTKSKYAYLNQPAIGSMDTPKRRSSSYVPNFCSYKLTPLQPTYLGVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E2I5</accession>
    <name>A0AA39E2I5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_002241</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E2I5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF78">
        <property type="entry name" value="OS12G0587133 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9707156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="614" mass="70655" checksum="AB9C4FE71957BAD3" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E2S8</accession>
    <name>A0AA39E2S8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_004663</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E2S8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36617">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36617:SF15">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase zinc-binding" evidence="1">
        <location>
            <begin position="203"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9702997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="350" mass="40670" checksum="8D85FC00D10014AD" modified="2024-01-24" version="1">MQRNFLWSGVGEGKRDHLVSWDVVCIPKAKGGLGLGRISLRNSALLGKWLWRFPREGSALWRHVILSIYGLHSNGWDANTVVRWSHRCPWKAIAQVSQEFFKFTRFVVGDGERIRFWEDLWWGDQPLGVRYPRLLSAVTDKNIPISSILGSTRPFSWNFNFRRNLSDSEIEDLESLMRSLDRLHLSPSGPDMRSWSLSPSRLFTVKSFFLTLSQIHGLPSDFPTRIVWNSQVPFKVKSFVWLVAHKKVNTNDMLQLRRPYKALSPDICMMCMKHGETVDHLFLHCPLTMGLWHKLFQLAKTDWVPPRSIFDMLSTNFNGFGSSKRGVVLWQAACIALIWVVWRERNVRIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E2T4</accession>
    <name>A0AA39E2T4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BZIP transcription factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_002891</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E2T4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006868">
        <property type="entry name" value="DUF630"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006867">
        <property type="entry name" value="DUF632"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21450">
        <property type="entry name" value="PROTEIN ALTERED PHOSPHATE STARVATION RESPONSE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21450:SF41">
        <property type="entry name" value="RNA POLYMERASE SUBUNIT BETA, PUTATIVE (DUF630 AND DUF632)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04783">
        <property type="entry name" value="DUF630"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04782">
        <property type="entry name" value="DUF632"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF630" evidence="3">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="DUF632" evidence="2">
        <location>
            <begin position="385"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="222"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="222"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="242"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9704545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="812" mass="91082" checksum="5C3DC6ACDCD8F6EA" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E3R9</accession>
    <name>A0AA39E3R9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Bet v I/Major latex protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_000460</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MLP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E3R9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07816">
        <property type="entry name" value="Bet_v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000916">
        <property type="entry name" value="Bet_v_I/MLP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052006">
        <property type="entry name" value="MLP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31338">
        <property type="entry name" value="POLYKETIDE CYCLASE/DEHYDRASE AND LIPID TRANSPORT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31338:SF16">
        <property type="entry name" value="POLYKETIDE CYCLASE_DEHYDRASE AND LIPID TRANSPORT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00407">
        <property type="entry name" value="Bet_v_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01037">
        <property type="entry name" value="Bet_v_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bet v I/Major latex protein" evidence="2">
        <location>
            <begin position="2"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Bet v I/Major latex protein" evidence="2">
        <location>
            <begin position="164"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9708431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="281" mass="31454" checksum="8CB62FE800589247" modified="2024-01-24" version="1">MAQICRLELQTEIKSSPDRIFDIYKNKTSLMPKISPDKLKSIEVLEGDGKSVGSVRLWTYVMGGAVIAKDKIVAIDEEKGSMTFDLIGGEVTNYYKSFKATIEATSEAHTNLVKWSLEYEKANETVPTPQSHLAFLLDVSKELLGRADPLYLMDNEKFDPCTGDTIGAKHTTFLISALIKHKVEVHEGDWETQGSVKHWSYTIGGNSVSRIKETTESIDEENRSITFRVLDGEVLKDYKSYKFTTQAILKGEGCLDGPDPHDYLELTVNVIKDIESHLLNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E530</accession>
    <name>A0AA39E530_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_000685</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E530"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044249">
        <property type="entry name" value="XERICO-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47258">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47258:SF3">
        <property type="entry name" value="F21J9.24-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="110"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9708788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="155" mass="16878" checksum="5B3128CE947F8F11" modified="2024-01-24" version="1">MGLSNFHSPAEGVLPVLVMNTVMSVALLKNMVRSVLQVMGANGNSPNLEEDPSNEEYSPMSENAKERRVSVTQFKSLCHSRSSVGRSENACSAGSSGTGTGSGWCSSMDCCVCLCRFEAEEEVSELSCKHFFHKGCLEKWFDNKHSSCPLCRSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E598</accession>
    <name>A0AA39E598_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Coiled-coil domain-containing protein SCD2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_001451</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E598"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000911">
        <property type="term" value="P:cytokinesis by cell plate formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040321">
        <property type="entry name" value="SCD2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31762">
        <property type="entry name" value="FAS-BINDING FACTOR-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31762:SF11">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="60"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="193"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="72"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="129"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="157"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="183"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9709976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="554" mass="61390" checksum="FDB8F0A2D5583342" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E6D9</accession>
    <name>A0AA39E6D9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HMA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_001765</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E6D9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22814:SF339">
        <property type="entry name" value="CHAPERONE, PUTATIVE, EXPRESSED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22814">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN ATOX1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMA" evidence="2">
        <location>
            <begin position="9"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9706417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="142" mass="16492" checksum="734D9F1334F0BFD0" modified="2024-01-24" version="1">MPKGRPLSLQTVELKVRMCCTGCERVVKNAIHKLRGVDKVEVNLEMEKVTVIGYVDRNKVLKAVRRSGKRAEFWPYPDPPLYFTSSNDYFKDLTNDYKESYNYWRHGYNLADRHGTIPPTHRGDDKVSNMFNDDNVNACCLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E8V5</accession>
    <name>A0AA39E8V5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein TSSC4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_001465</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9710004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E8V5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029338">
        <property type="entry name" value="TSSC4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13445:SF5">
        <property type="entry name" value="PROTEIN TSSC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13445">
        <property type="entry name" value="TUMOR SUPPRESSING SUBTRANSFERABLE CANDIDATE 4 TSSC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15264">
        <property type="entry name" value="TSSC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="27"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9710004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="469" mass="51984" checksum="B93FFFF177DDEA85" modified="2024-01-24" version="1">MASSQEPDDDFGVRVEKVFRSFKPVHSSDSTSLWSVSDTEVEKRDQNREGKKGTEKDEENDVFLGSEDREGLEAESGDGRREADGGFEDRGDGGVRGGVENAGAGDGEEWEIRSSIGMDRTLDDEEEEDLFDKVAVGRENAGDRIYMRDATDHGTGLNSHNVLTQSHDATKDPRIDHFAAQIGLKEDKLEAQNFNSPQSCCKTALDVKVPQHQGSAHGGVKSILKRKGDTITPKSQKRVKFDLSRNDYNEAPTRAKDFFSTDASLVEIEVSEDDSPLTRNTRGVPDYVLNSSKYTHYSLDSSSDVIEDSKSQACVELLEQMKRSKAEELGLELKDNSVDLPKSLTFIPKKKVCDAKVADSSSQKKNQDAGCKGSLHQTGFPIGIAAGEVHQTEAGIDEDPDTIPADKSPVQALPECHDSVYDSYLRSTFFFFQIISSFFQVFFHVHFCWSMLRFGPLITQVIILIRAFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39EDW9</accession>
    <name>A0AA39EDW9_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_001420</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ARG7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39EDW9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009733">
        <property type="term" value="P:response to auxin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003676">
        <property type="entry name" value="SAUR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31374">
        <property type="entry name" value="AUXIN-INDUCED PROTEIN-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31374:SF118">
        <property type="entry name" value="OS01G0924966 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02519">
        <property type="entry name" value="Auxin_inducible"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9709927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="158" mass="18070" checksum="1A59201BDAD21484" modified="2024-01-24" version="1">MDETKSRQKKGLMKKTWEKFKSFGHGRVLSRTHHSSMKSKSRPGHTASLEGVKKGRVAPEGCFSVYVGHGKQRFVVKTEYANHPLFRALLEEAELEYGYNNGVFAFWPLLLSCKMEIFLKVLLEMDSSDEVHQGCSFARNPSSYRLLSPSRMITMNHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YHA4</accession>
    <name>A0AA38YHA4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_026768</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YHA4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18919">
        <property type="entry name" value="bHLH_AtBPE_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000002">
        <property type="entry name" value="Basic helix-loop-helix transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024097">
        <property type="entry name" value="bHLH_ZIP_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12565">
        <property type="entry name" value="STEROL REGULATORY ELEMENT-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12565:SF458">
        <property type="entry name" value="TRANSCRIPTION FACTOR BHLH49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="5">
        <location>
            <begin position="253"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9670437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="416" mass="45741" checksum="A305E395F2672D73" modified="2024-01-24" version="1">MDTDGSGDSGFEHKDGSLLDCPSLGIFTSLSSLNGATSIFTGDYDPPVPLAHNADIIGPPPLTTHDQIDDLYAGNVLENQGMSRTIHLTQYSSDPRFVELVPNFPHFSTAGFSETVTSSCLPRCHNIGCPPDHPLEKNGTRKTSNLRNKTNNCAQMKEGCHIADDGTMELSPIEKKRKRLADDRSQFAHLKNMEAAQQKDECQGRQDERKQKAEQKIVANNCGKLIGEEVKISSQTGEAPKEDYIHVRAKRGQATNSHSLAERVRRERISERMKFLQDLVPGCNKITGKAVMLDEIINYVQSLQRQVEFLSMKLATVYPEMNVQIERILSSDIQHSKGGTAPILGFGPGMNSAYPIPRVTLQAISPAIESSTLQSSPMSPMPNVWDNELQGIKQMGFTSTAALENLETSGCTKVQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YHV7</accession>
    <name>A0AA38YHV7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">HSF-type DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_026966</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the HSF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YHV7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000037">
        <property type="entry name" value="Heat stress transcription factor B-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF329">
        <property type="entry name" value="HEAT STRESS TRANSCRIPTION FACTOR B-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00056">
        <property type="entry name" value="HSFDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00434">
        <property type="entry name" value="HSF_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0346">Stress response</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HSF-type DNA-binding" evidence="11">
        <location>
            <begin position="49"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="98"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="212"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="121"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="160"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="212"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="224"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9670756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="286" mass="31629" checksum="BE20E250410BA5F3" modified="2024-01-24" version="1">MAQRSVPAPFLTKTYQLVDDPSTDDVISWSESGNTFVVWKTADFAKDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDKWEFANEYFKRGQRELMSEIRRRKTTTSATGQAPPGGKSAGGGTSSPTNSGEDLGSTSTSSPDSKNPGSVETTTTAQFADLSDENEKLKKDNESLSTELAQTKRQCEELISFLTEYVKVAPDQINSIMKQGSCGSGHGGLVGEIGGSVDDDDDDEKDEEPNEESLKLFGVWLKGKKKRGREEKMGYGGPHQKEMKTVDFEAPWMTSKVCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YHY9</accession>
    <name>A0AA38YHY9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Patatin</fullName>
            <ecNumber evidence="7">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_026980</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Lipolytic acyl hydrolase (LAH).</text>
    </comment>
    <comment type="domain">
        <text evidence="7">The nitrogen atoms of the two glycine residues in the GGXR motif define the oxyanion hole, and stabilize the oxyanion that forms during the nucleophilic attack by the catalytic serine during substrate cleavage.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the patatin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="7"/>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YHY9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004620">
        <property type="term" value="F:phospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07214">
        <property type="entry name" value="Pat17_isozyme_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1090.10:FF:000005">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32176">
        <property type="entry name" value="XYLOSE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32176:SF92">
        <property type="entry name" value="XYLOSE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6 7" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="5 6 7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNPLA" evidence="8">
        <location>
            <begin position="17"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXGXXG" evidence="6">
        <location>
            <begin position="21"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="6">
        <location>
            <begin position="59"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DGA/G" evidence="6">
        <location>
            <begin position="211"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9670775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="405" mass="44983" checksum="8EB6E89BC1913FDE" modified="2024-01-24" version="1">MSLQIKPPAQGNLITVLSIDGGGIKGIIPGIMLDFLESELQKLDGEDARLADYFDVVSGTSTGGLIATMLTAPNENNRPFYAASEIKPFYLENSPKIFPQKSGILASVENLFKAFTGPKYDGKYLHSLLKNELRDTRLHQTLTNVVIPTFDIKELQPTIFSSYQLTTTPSLDAKLADICISTSAAPTFLPAYYFENPDEKGAKMREFNLTDGGVAANNPTLLAISEVMKQESNKNPDFSPFKPMEYDRLLVISLGTGSAKKEHKYNGKMASKWGFAEWLYNDGNTPLVECFAQASTDMVDFYISLAFQTHQYEENYLRIDDDTLTGTLASLDVATKENLNNLVQVGEKLLKKPVSRVNLETGLYEPVKNGGSNENALRKFAMKLSDERRLRESMAHPAQDPEYII</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YKI1</accession>
    <name>A0AA38YKI1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Purple acid phosphatase</fullName>
            <ecNumber evidence="9">3.1.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_025658</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a phosphate monoester + H2O = an alcohol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15017"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:67140"/>
            <dbReference type="EC" id="3.1.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4 9">Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.2" evidence="9"/>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YKI1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00839">
        <property type="entry name" value="MPP_PAPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.380">
        <property type="entry name" value="Purple acid phosphatase-like, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041792">
        <property type="entry name" value="MPP_PAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039331">
        <property type="entry name" value="PAPs-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025733">
        <property type="entry name" value="PAPs_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015914">
        <property type="entry name" value="PAPs_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008963">
        <property type="entry name" value="Purple_acid_Pase-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22953">
        <property type="entry name" value="ACID PHOSPHATASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22953:SF155">
        <property type="entry name" value="PURPLE ACID PHOSPHATASE 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14008">
        <property type="entry name" value="Metallophos_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16656">
        <property type="entry name" value="Pur_ac_phosph_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49363">
        <property type="entry name" value="Purple acid phosphatase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041488196" description="Purple acid phosphatase" evidence="9">
        <location>
            <begin position="20"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="domain" description="Purple acid phosphatase N-terminal" evidence="12">
        <location>
            <begin position="42"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="10">
        <location>
            <begin position="137"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="Purple acid phosphatase C-terminal" evidence="11">
        <location>
            <begin position="342"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF14008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF16656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9672095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="432" mass="48992" checksum="48F77D340CA563BF" modified="2024-01-24" version="1">MEPKLILILILVISVSVTADYVRPKPRKALHFPWQPKASSLPQQVHISLSSEKHMRITWITGDEYAPSIVQYGTSPGKYTSMTLGGSTSYSYLFYSSGKIHHTVIGPLEHDTIYYYRCGGQGPEFQLKTPPAQFPITFAVAADLGQTGWTKSTLDHIDGCNYDVHLLPGDLSYADYLQRRWDTFGELVQPLASARPWMVTEGNHEQENIPFFKDGFESYNSRWTMPYQESGSPSNLYYSFEVAGVHVVMLGSYAAYDLYSNQYSWLKTDLSRVDRKRTPWLLVLFHVPWYNSNKAHQGEGDRMMETLEPLLYAANVDLVLAGHVHAYERSKRVYNGKSDPCGPIHITIGDGGNREGLATRYNDPQPEWSVFREASFGHGELKIVNSTHAFWSWHRNDDDEPVRSDEVWITSLVGSGCNAGNRHGQRKILMEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YKV9</accession>
    <name>A0AA38YKV9_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_025805</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YKV9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9672334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="63" mass="6989" checksum="13AE3D39C1E65EAE" modified="2024-01-24" version="1">MPRHTRKLISFSVGLGEDKSQEKFPSTISIAVSELPFGSLSITSLGRSSRRRKDVILSRDTLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YL06</accession>
    <name>A0AA38YL06_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DEK-C domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_025848</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YL06"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000779">
        <property type="term" value="P:regulation of double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000220">
        <property type="entry name" value="DEK domain-containing chromatin associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044198">
        <property type="entry name" value="DEK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014876">
        <property type="entry name" value="DEK_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13468:SF22">
        <property type="entry name" value="DEK DOMAIN-CONTAINING CHROMATIN-ASSOCIATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13468">
        <property type="entry name" value="DEK PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08766">
        <property type="entry name" value="DEK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109715">
        <property type="entry name" value="DEK C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51998">
        <property type="entry name" value="DEK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="327"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="DEK-C" evidence="9">
        <location>
            <begin position="546"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="358"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="603"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="19"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="44"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="136"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="361"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="380"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="396"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="425"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="453"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="524"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="606"/>
            <end position="618"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9672387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="630" mass="71668" checksum="94739DCB5BAA2266" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YLS0</accession>
    <name>A0AA38YLS0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cytochrome P450 CYP72A219-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_026074</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YLS0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000029">
        <property type="entry name" value="Cytochrome P450 734A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050665">
        <property type="entry name" value="Cytochrome_P450_Monooxygen"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24282:SF255">
        <property type="entry name" value="CYTOCHROME P450 72A11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24282">
        <property type="entry name" value="CYTOCHROME P450 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11 12" id="KW-0349">Heme</keyword>
    <keyword evidence="8 11 12" id="KW-0408">Iron</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 12" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="11">
        <location>
            <position position="443"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9672719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="495" mass="56754" checksum="C8FAB97440596775" modified="2024-01-24" version="1">MKLSSVAISFAFITLLIYAWRLLNWVWLRPKKLERCLRQQGLTGNSYRLLHGDFREMLRMINEANSRPISLSDDIVQRVLPFHCHSIKKYADKGCFLEVQCLHKPAPHPLGKLLATGLVTLEGEQWAKRRKIINPAFHLEKLKHMVPAFQLSCSDLVNKWEKKLSKDGSCELDIWPDLENLAGDAISRTAFGSSYEEGRRIFQLQKEQAHLAVKVFRSVYIPGWRFVPTKTNKRMRQISNEVHALLKGIIERREKAMKVGKTVNDDLLSLLMESNFREMQVHDERKNVGMSIKDVIEECKLFYFAGQETTSDLLLWTMVLLSKHSNWQARAREEVLQVFGNKNPDGDGLNHLKIVTMIFHEVLRLYPPASTLIRTVLPDSQVGGWYLPDGVHIALPILLIHHDHEIWGEDAKEFNPERFSEGVSKATKGQFAFFPFGYGPRVCIGQNFAMMEAKMALAMILQRFSFELSPSYAHAPCNIITIQPQYGAHLILHGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YLX6</accession>
    <name>A0AA38YLX6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Translation initiation factor IF-2, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_026226</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YLX6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01887">
        <property type="entry name" value="IF2_eIF5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03702">
        <property type="entry name" value="IF2_mtIF2_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03692">
        <property type="entry name" value="mtIF2_IVc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000008">
        <property type="entry name" value="Translation initiation factor IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000054">
        <property type="entry name" value="Translation initiation factor IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10050:FF:000001">
        <property type="entry name" value="Translation initiation factor IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000019">
        <property type="entry name" value="Translation initiation factor IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10050">
        <property type="entry name" value="Translation initiation factor IF- 2, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00100_B">
        <property type="entry name" value="IF_2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053905">
        <property type="entry name" value="EF-G-like_DII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044145">
        <property type="entry name" value="IF2_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006847">
        <property type="entry name" value="IF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000178">
        <property type="entry name" value="TF_IF2_bacterial-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015760">
        <property type="entry name" value="TIF_IF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023115">
        <property type="entry name" value="TIF_IF2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036925">
        <property type="entry name" value="TIF_IF2_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00487">
        <property type="entry name" value="IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381:SF5">
        <property type="entry name" value="TR-TYPE G DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR IF-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22042">
        <property type="entry name" value="EF-G_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11987">
        <property type="entry name" value="IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04760">
        <property type="entry name" value="IF2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52156">
        <property type="entry name" value="Initiation factor IF2/eIF5b, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01176">
        <property type="entry name" value="IF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="10">
        <location>
            <begin position="486"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="147"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="214"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="314"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="745"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="147"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="160"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="216"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="231"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9672886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1015" mass="108320" checksum="A5EF720C69135F3A" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YME8</accession>
    <name>A0AA38YME8_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_026360</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9673049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YME8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF78">
        <property type="entry name" value="OS12G0587133 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9673049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="194" mass="22200" checksum="06FAD952DF54B349" modified="2024-01-24" version="1">MPLGAPFKSIGVWDGIEERFRKRLAMWKRQYISKGGRITLIRSTLSNLPIYYMSIFHLPRAVRLRLEKIQRDFLWGGGSLEQKPHLVRWSIVCEDKSKGGLGVKSLGLFNKALLGKWAWRFANEKKALWNQVIRRKYGEEIGGWRSCENREAFGVGMWKAINKVGQLVTPYFGFEVGDGKNVRFGKISGVVPAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YNZ0</accession>
    <name>A0AA38YNZ0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pesticidal crystal cry8Ba protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_023498</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YNZ0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31110">
        <property type="entry name" value="PESTICIDAL CRYSTAL CRY8BA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31110:SF2">
        <property type="entry name" value="PESTICIDAL CRYSTAL CRY8BA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="162"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="221"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="125"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="179"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="223"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="255"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9673981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1255" mass="139350" checksum="2F7ECD5C3CD35BFF" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YR56</accession>
    <name>A0AA38YR56_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Chlororespiratory reduction 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_024190</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. PCMP-H subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YR56"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000333">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000511">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF531">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="76"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="138"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="200"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="302"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="337"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9675141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="525" mass="59029" checksum="D433FEEE09158EE2" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YSY1</accession>
    <name>A0AA38YSY1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_024883</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NADH:flavin oxidoreductase/NADH oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9676098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YSY1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016629">
        <property type="term" value="F:12-oxophytodienoate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02933">
        <property type="entry name" value="OYE_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000073">
        <property type="entry name" value="12-oxophytodienoate reductase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001155">
        <property type="entry name" value="OxRdtase_FMN_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045247">
        <property type="entry name" value="Oye-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22893">
        <property type="entry name" value="NADH OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22893:SF91">
        <property type="entry name" value="NADPH DEHYDROGENASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00724">
        <property type="entry name" value="Oxidored_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0288">FMN</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH:flavin oxidoreductase/NADH oxidase N-terminal" evidence="7">
        <location>
            <begin position="19"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9676098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="379" mass="41923" checksum="9827EB8D69A24956" modified="2024-01-24" version="1">MDGKVQGINGVSAETPIPLITPYKLGMFQLSHRVVLAPLTRQRSWNNVPQPHAILYYSQRTSKGGLLIAEATGVSDTAQGYPDTPGIWTKEQVEAWKPIVDAVHAKGGILFCQIWHVGRVSNTDFQPNGQAPISCTDKPLTPQIRTNGIDVDQFSPPRRLTTDEIPQVVKDFRLAARNAIEAGFDGVEIHGAHGYLLDQFMKDQVNDRTDKYGGSLENRCRFPLEVVEAVVDEIGADKVGIRLSPFAAYGEAGDSNPKALGLYMAESLNKYGLLYCHMVEPRMKTLGEKCGSAHSLLPMRKAFNGTFLVAGGYDREDGNSAVAENRADLVVYGRWFLANPDLPKRFALNAPLNKYNRETFYTPDPVVGYTDYPFLEDTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YV83</accession>
    <name>A0AA38YV83_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cytochrome P450 CYP736A12-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_022179</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YV83"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11072">
        <property type="entry name" value="CYP71-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000011">
        <property type="entry name" value="Cytochrome P450 83B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47943:SF9">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47943">
        <property type="entry name" value="CYTOCHROME P450 93A3-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10 11" id="KW-0349">Heme</keyword>
    <keyword evidence="7 10 11" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 11" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041421266" description="Cytochrome P450 CYP736A12-like" evidence="12">
        <location>
            <begin position="22"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="10">
        <location>
            <position position="442"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9677054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="502" mass="56490" checksum="21420EAA8FD8E3CA" modified="2024-01-24" version="1">MALFTVAILLVILGAMWVTLSHQKLRAGYRKLPPGPWGLPIIGCLHMLGSLPHRNLSRLAKKYGPIMFMRLGCVPTIVVSSPEAAKLVMKTHDVVFASRPKLQAYEYLSYGAKGVAFTEYGPYWRHVRKLCALELFSSAKINSFASMRKEEVGLLVKSVKEMASAGEVVDISAKVAGVVEDMAYRMVFGHNKDRIIDLKTLIREATRLAGIFNLADYVPFLGPLDLQGLTQLLRTNSEATDEVLDKIIDNHVQDVSKDEVNHMNFIDVVLSLMNKSNNFGDELLYAIDRKNAKAIILDMLVGGIDSSITSIEWVLSELLRHPRVMGRLQEELKNVVDMCRMVEESDLENLGYLNMVVKETLRLHPIGPFLVPRESIEDIVINGHYIPKKSRILINTWTIGRDPNVWSNNVEEFFPERFAENNIDLQGHDFELTPFGSGRRGCPGIQLGLTTVKLVVSQLVHCFNWKLPNDTPPSELNMKEKFGLTMPRVDHLLAMPTYRLPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YV98</accession>
    <name>A0AA38YV98_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_022363</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YV98"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009500">
        <property type="entry name" value="DUF1118"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06549">
        <property type="entry name" value="DUF1118"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="160"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9677341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="193" mass="20484" checksum="BF19B20CF8ECC2D4" modified="2024-01-24" version="1">MAVTAASPAAFLRQLEHNLYPTKSRFRRDFPAKKPLRILAMASKKKVNRYDENWKKEWFGAGIFVEGSEEVEVDVFKNLEKKKVLSNVEKAGLLSKAEDLGFTLSSIEKLGVFSKAEDLGLLSLLEKAASFSPSALASAALPIFVAAIAAIVLIPDDSAALVAIQAVIAGALVVGATGLVVGSVVLGGLQEAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YWD7</accession>
    <name>A0AA38YWD7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cytochrome P450 71A1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_022731</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YWD7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11072">
        <property type="entry name" value="CYP71-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000043">
        <property type="entry name" value="Cytochrome P450 99A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47955:SF19">
        <property type="entry name" value="CYTOCHROME P450 71A9-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47955">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 71 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0349">Heme</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9677925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="495" mass="56731" checksum="C425F273383B1E8D" modified="2024-01-24" version="1">MALLQWLKEGFLPSFLFAGVILVVVLKFLQKGMLRKRKFDLPPSPRKLPIIGNLHQLGNMPHISLHRLAQKFGPIIFLQLGEVPTVVVSSARVAKEVMKTHDLALSSRPQIFSAKHLFYDCTDIVFSPYGAYWRHIRKICILELLSAKRVQSFSFVREEEVARMVHRIAESYPCPTNLTKILGLYANDVLCRVAFGRDFSAGGEYDRHGFQKMLEEYQVLLGGFSVGDFFPSMEFIHSLTGMKSRLQNTFRRFDNFFDQVVKEHLDPERKKEEHKDLVDVLLHVKEEGATEMPLTMDNVKAIILDMFAAGTDTTFITLDWGMTELIMNPKVMERAQAEVRSIVGERRVVTESDLPQLHYMKAVIKEIFRLHPPAPVLVPRESMEDVTIDGYNIPAKTRFFVNAWAIGRDPESWQNPESFEPQRFMGSTIDFKGQDFELIPFGQQLLSLLLLNFSTAFDWELPPGIQVQDLDMTEVFGITMHRIANLIVLAKPRFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YWN8</accession>
    <name>A0AA38YWN8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein DETOXIFICATION</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Multidrug and toxic compound extrusion protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_022699</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YWN8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13132">
        <property type="entry name" value="MATE_eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045069">
        <property type="entry name" value="MATE_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="52"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="127"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="157"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="190"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="219"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="263"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="343"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="380"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="416"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="446"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="479"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9677877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="543" mass="59912" checksum="54CD2BFD74E31C1C" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YX97</accession>
    <name>A0AA38YX97_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Methyltransferase type 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_020542</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the class I-like SAM-binding methyltransferase superfamily. gTMT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YX97"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025774">
        <property type="entry name" value="PiNMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591:SF81">
        <property type="entry name" value="MAGNESIUM PROTOPORPHYRIN IX METHYLTRANSFERASE, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51581">
        <property type="entry name" value="SAM_GTMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="5">
        <location>
            <begin position="128"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="SAM motif I" evidence="4">
        <location>
            <begin position="127"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="SAM motif II" evidence="4">
        <location>
            <begin position="190"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="SAM motif III" evidence="4">
        <location>
            <begin position="217"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9678393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="345" mass="38204" checksum="6FC497FEE578ECC0" modified="2024-01-24" version="1">MAILPASCTYHLSPLLQLPQRLASQPYSWPRPAVTAPTSRRFARLTAAALSTMSPEEASLKKGIAELYDQSSGLWEDIWGDHMHHGFYEPDSAASDADHRFAQIRMIEESLRFAGVSEEEEKRPKRVVDVGCGIGGSSRYLAKKYGASCQGITLSPLQAQRAQTLAASQGLADKVSFQVADALDQPFPDGQFDLVWSMESGEHMPDKKKFVGELARVAAPGGTIILVTWCHRDLSPSEESLKPEEKALLDKICSAYYLPDWCSTTDYVKLLESLSLQDIKAADWSDYVAPFWPAVIRSALTFKGFISLLRSGWKTIRGALVMPLMIQGYKKGLIKFAIITCRKPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YYV1</accession>
    <name>A0AA38YYV1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Hydroxyproline-rich glycoprotein family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_021150</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YYV1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37767">
        <property type="entry name" value="HYDROXYPROLINE-RICH GLYCOPROTEIN FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37767:SF1">
        <property type="entry name" value="HYDROXYPROLINE-RICH GLYCOPROTEIN FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="167"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="181"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9679130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="321" mass="35246" checksum="62D5BB66816557EF" modified="2024-01-24" version="1">MTEQQVSSYRKQTRQPPSVPFLWEEKPGIPKKDWKPEVTAVNPAPPPLPPPPPPPPPPPPPPLPIKLIASIPFTWEEKPGKPLPFFSGTPHDDSFLLFPPKKLVCCSSLSEADSKDYEDDGDDEHDGIFESDYEAFGFETDDSFSSAPSLLANRLMSTVAISTTVPVQKTSLNEDSNDQPESPSSPASETNSSTSSNATGTTSLVGSSFLHCLFPLFPPNSGFLAKVGCPEGSPPPPELQNKGLDRETNSSVIVRRAPTLGELIMMSRRRSYRRKAVQMRKHNLLVEFTKKRAFGCWIFGTGGRRIGGLLGKKRQPRLKLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YZ98</accession>
    <name>A0AA38YZ98_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Calmodulin-binding heat-shock protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_021400</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YZ98"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00519">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002921">
        <property type="entry name" value="Fungal_lipase-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005592">
        <property type="entry name" value="Mono/diacylglycerol_lipase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46398">
        <property type="entry name" value="ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46398:SF4">
        <property type="entry name" value="ALPHA_BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03893">
        <property type="entry name" value="Lipase3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01764">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mono-/di-acylglycerol lipase N-terminal" evidence="4">
        <location>
            <begin position="9"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="Fungal lipase-type" evidence="3">
        <location>
            <begin position="109"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="393"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="407"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9679456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="453" mass="51523" checksum="69BC93FFBAF4CAA9" modified="2024-01-24" version="1">MSIVCGIPLLECVYCLACARWAWKRCLHTAGHDSETWGLSTAEEFEPVPRLCRYILAVYEEDLRHPLWEPAGGYEINPDWLILKKTYEDTKGCAPPYILYLDHKHADIVLAIRGLNLAKESDYAVLLDNKLGQRKFDGGYVHNGLLKAAGWVLDAECDVLRELVEKYPNYTLTFTGHSLGSGVAALLTMVVVHNRDKLANIDRKRIRCYAIAPARCMSLNLAVRYADLINSVVLQDDFLPRTATPLEDIFKSLFCLPCLLCLRCMKDTCIPEEKMLRDPRRLYAPGRLYHIVERKPFRTGRFPPVVRTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAQRALDLMLEKDKIMEIPAKQKMERQETLKREHSEEYKAALQRAVSLAVPHAYSPSQYGTFSDHEEGENSSRSSEGSSYGSSKTSKKKESWDEVIERLFEKDESGLMVLKKPNDGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YZB2</accession>
    <name>A0AA38YZB2_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_021421</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0496 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YZB2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007749">
        <property type="entry name" value="DUF677"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31113:SF6">
        <property type="entry name" value="UPF0496 PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31113">
        <property type="entry name" value="UPF0496 PROTEIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05055">
        <property type="entry name" value="DUF677"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="205"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="301"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9679488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="365" mass="40648" checksum="0A1184699C4B14B3" modified="2024-01-24" version="1">MVGMKEKREQESRSSLTSPLMPLKPCTEEQVSGSNPPNLPNYADYREEYANAFRTESYIEFWTRVLTLTHGDSATSIPIESTTAARLSSYRLFAEYLLDPDQPTVTQLLALAQSRPKNHSILAEYFSETANASFLCSLLLKDINRIRVWYRSLKSTLESHGATELLPVNRFPVILARVQVIQTGCSGLLKRLELGRDKARAKLQLVNSIKCGSAILLVALTASVAVIATTHALAMLVAAPGLIAASLELASTRKLARLPAQLDAAAKGTYILNKDMDMISRLVARLNNELEHISGMVRFWLEHKEDNLQASGEVARQLKKNDSNFSQQLDELEEHLYLCFMTINRGRTLVVKEIMDQGLPNVSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YZY3</accession>
    <name>A0AA38YZY3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycosyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_021495</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="5"/>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YZY3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035251">
        <property type="term" value="F:UDP-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000020">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000095">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050481">
        <property type="entry name" value="UDP-glycosyltransf_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48048">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48048:SF30">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9679587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="462" mass="50547" checksum="2A8F640B4EBBB813" modified="2024-01-24" version="1">MKNVVLYPSPGMGHLISMVELGKLILKHHPSLCITILTITAPFDTGATGSYISTVSSTTPSINFHHLPITPLPQDPSSYPTFETISYELLTIHNPNVHQALRAISGNSNVLALVIDFFCTPALTVAEQLNIPAYYFFTSSSAGLALFLYIPTLHRNNAQSFKDIMNTFLQIPGLPPIPSADMPAPLMDRTSKEYESFLYYTTHAPKSAGIIVNTFESLEPKALKAVRDGLCVTDGPTPPVFSIGPLIATHGGDGGEHGKKCLKWLDSQSKRSVVFLCFGSMGFFSEEQLKEIAVGLERSGQRFLWVVRSPPSKDQSRRFSAPPDPDLGSLLPDGFLERTQERGLVVKSWAPQVAVLSHISVGGFVTHCGWNSVLEAVSSGVPMVGWPLYAEQRFNKVALESEGGLVTAIEVEKRVKELMETEKGFSIRSRITALKEEARAAISDGGSSLAELDKLIKFWRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z1K7</accession>
    <name>A0AA38Z1K7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transcription termination factor MTEF18, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_019803</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the mTERF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z1K7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.70.10:FF:000017">
        <property type="entry name" value="Transcription termination factor MTEF18, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.70.10">
        <property type="entry name" value="Transcription termination factor 3, mitochondrial"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003690">
        <property type="entry name" value="MTERF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038538">
        <property type="entry name" value="MTERF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13068">
        <property type="entry name" value="CGI-12 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13068:SF113">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR MTEF18, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02536">
        <property type="entry name" value="mTERF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00733">
        <property type="entry name" value="Mterf"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0806">Transcription termination</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9680599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="561" mass="64311" checksum="3055C22162684756" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z354</accession>
    <name>A0AA38Z354_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mechanosensitive ion channel MscS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_020307</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MscS (TC 1.A.23) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9681362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z354"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034220">
        <property type="term" value="P:monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023408">
        <property type="entry name" value="MscS_beta-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011014">
        <property type="entry name" value="MscS_channel_TM-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30566:SF5">
        <property type="entry name" value="MECHANOSENSITIVE ION CHANNEL PROTEIN 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30566">
        <property type="entry name" value="YNAI-RELATED MECHANOSENSITIVE ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82861">
        <property type="entry name" value="Mechanosensitive channel protein MscS (YggB), transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="199"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="259"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="284"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="335"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="Mechanosensitive ion channel MscS" evidence="9">
        <location>
            <begin position="373"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9681362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="540" mass="59320" checksum="345E38D0CD18C179" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z533</accession>
    <name>A0AA38Z533_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_018464</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z533"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9682546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="104" mass="11437" checksum="450FD021D53EFFAD" modified="2024-01-24" version="1">MWLVIGMLVYGFYGRRCSSPQDAIHVPAVDVDEIYGSFSNCSDDSSVVISALCINNSEFASCSGWYSQPECHISDHPWHEGTASSCTATKFNSVEDCRDFRGPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z7C7</accession>
    <name>A0AA38Z7C7_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_016324</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9683775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z7C7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9683775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="84" mass="9344" checksum="31A36B7AE476AD1C" modified="2024-01-24" version="1">MCPDISRQVEELLQKGGQRRKGCHVSVVEAGHMLGRVIGWRRGDAELDATTAREESWRTRGPNHGKGISEFEGVNAENESKQVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z827</accession>
    <name>A0AA38Z827_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transducin/WD40 repeat-like superfamily protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_016313</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9683757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z827"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057221">
        <property type="entry name" value="DUF7899"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31789">
        <property type="entry name" value="OS05G0482600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31789:SF1">
        <property type="entry name" value="OS05G0482600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25463">
        <property type="entry name" value="DUF7899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69322">
        <property type="entry name" value="Tricorn protease domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9683757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="482" mass="54990" checksum="CD6003C21790ACDE" modified="2024-01-24" version="1">MMEGKRIAVNPRPGGGRRVVAKKRPRNDGFVNSVKKLQRREICSKRDRSFSMSDAQERFRNIRLQEEYDTHDPKGHCTLVLPFLRKRSKIIEIVAARDIVFALAQSGVCAAFSRETNQRICFLNVSPDEVIRSLFYNKNNDSLITVSVYASDNFSSLKCRTTRIEYIRRGKPDAGFALFESESLKWPGFVEFDDVNGKVLTYSAQDSIYKVFDLKNYTMLYSISDKHVQEIKISPGIMLLIFTKASSHVPLKILSIEDGTVLKSFNHLLHRNKKVDFIEQFNEKLLVKQENENLQILDVRNSELKEVSRTEFMTPSAFIFLYENQLFLTFRNRTVAVWNFRGELVTSFEDHLLWHPDCNTNNIYITSDQDLIISYCKADSDDPLSEGNAGSINISNILTGKCLAKIKASNNFPMENECSCSSNFSSSSSSIGGSKRRAHASASRIRSTVAEALEDITALFYDEERNEIYTGNRHGLVHVWSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z8X8</accession>
    <name>A0AA38Z8X8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_016862</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z8X8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000132">
        <property type="entry name" value="caffeoylshikimate esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051044">
        <property type="entry name" value="MAG_DAG_Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11614">
        <property type="entry name" value="PHOSPHOLIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="1">
        <location>
            <begin position="58"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9684600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="329" mass="36116" checksum="13B60B3D75F22A75" modified="2024-01-24" version="1">MVHPVAEANEQSPFGSLSPSEFYARHSVTHSSEYITNSRGMKLFTQSWTPLPPTKIIGTLAVVHGFTGESSWFVQLTAVHFTKAGFATCAIDHQGHGFSDGLVAHIPDINPVVDDCIAFFDSFRARHAPSLPSFLYSESLGGAIALLITLRRGPSRPWDGLVLNGAMCGISPKFKPPWPLEHFLFLLAAVVPTWRVVPTRGALPQLSFKVEWKRNLALASPRRPVARPRAATALELLRVCREIQNRYDEVEVPFLVVHGGDDVVCDPACVEELYRRAPSKDKTLKIYPDMVHQLVGEPDENVELVFGDIVEWLRTRAESAAAGGGDGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z9P6</accession>
    <name>A0AA38Z9P6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 10">Uricase</fullName>
            <ecNumber evidence="7 10">1.7.3.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Urate oxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_017151</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 10">Catalyzes the oxidation of uric acid to 5-hydroxyisourate, which is further processed to form (S)-allantoin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 10">
            <text>urate + O2 + H2O = 5-hydroxyisourate + H2O2</text>
            <dbReference type="Rhea" id="RHEA:21368"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
            <dbReference type="ChEBI" id="CHEBI:18072"/>
            <dbReference type="EC" id="1.7.3.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Purine metabolism; urate degradation; (S)-allantoin from urate: step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7 10">Belongs to the uricase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.7.3.3" evidence="7 10"/>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z9P6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004846">
        <property type="term" value="F:urate oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006145">
        <property type="term" value="P:purine nucleobase catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019628">
        <property type="term" value="P:urate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00445">
        <property type="entry name" value="Uricase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.270.10:FF:000001">
        <property type="entry name" value="Uricase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.270.10">
        <property type="entry name" value="Urate Oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002042">
        <property type="entry name" value="Uricase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03383">
        <property type="entry name" value="urate_oxi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42874">
        <property type="entry name" value="URICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42874:SF1">
        <property type="entry name" value="URICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01014">
        <property type="entry name" value="Uricase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000241">
        <property type="entry name" value="Urate_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00093">
        <property type="entry name" value="URICASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55620">
        <property type="entry name" value="Tetrahydrobiopterin biosynthesis enzymes-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6 7" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="4 7 10" id="KW-0659">Purine metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Charge relay system" evidence="8">
        <location>
            <position position="17"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8">
        <location>
            <position position="266"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>5-hydroxyisourate</name>
            <dbReference type="ChEBI" id="CHEBI:18072"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>O2</name>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>urate</name>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>5-hydroxyisourate</name>
            <dbReference type="ChEBI" id="CHEBI:18072"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>urate</name>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>5-hydroxyisourate</name>
            <dbReference type="ChEBI" id="CHEBI:18072"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>urate</name>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>5-hydroxyisourate</name>
            <dbReference type="ChEBI" id="CHEBI:18072"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>urate</name>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>5-hydroxyisourate</name>
            <dbReference type="ChEBI" id="CHEBI:18072"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>urate</name>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>5-hydroxyisourate</name>
            <dbReference type="ChEBI" id="CHEBI:18072"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>urate</name>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>5-hydroxyisourate</name>
            <dbReference type="ChEBI" id="CHEBI:18072"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>O2</name>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>urate</name>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000241-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000241-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9685005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="309" mass="34877" checksum="92B2AF992FB63C7D" modified="2024-01-24" version="1">MASEKIDGFTLEQRHGKARVRLGRVWRSQSGRHIFVEWTVSVSLLSNCLAAYVRDDNSDIVATDSMKNTVYAKAKECAQQLSMEEFAIKLAKHFTSFYQQVTTAIVTIEEKPWERAYIDGQPHDHGFKRGSETHTTEVIVEKSGALRMTSGIQGLALLKTTQAGFEGFIRDKYTVLPDTRERIVATEVTASWRYPFESLSGIPLQPLYFTEKYLDVKKVLVETFFGPPRGGVYSPSVQSTLYQMAKTVLNRFPDISSIQLKMPNLHFLPVNISSKDNPAIVKFDDDVYLPTSEPHGSIEATLSRIRAKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZA02</accession>
    <name>A0AA38ZA02_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GPI inositol-deacylase</fullName>
            <ecNumber evidence="1">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_017270</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in inositol deacylation of GPI-anchored proteins which plays important roles in the quality control and ER-associated degradation of GPI-anchored proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GPI inositol-deacylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="1"/>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZA02"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012908">
        <property type="entry name" value="PGAP1-ab_dom-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47346:SF1">
        <property type="entry name" value="GPI INOSITOL-DEACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47346">
        <property type="entry name" value="HYDROLASES, ACTING ON ESTER BOND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07819">
        <property type="entry name" value="PGAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0653">Protein transport</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="679"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="728"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="762"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="807"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="831"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="940"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1029"/>
            <end position="1047"/>
        </location>
    </feature>
    <feature type="domain" description="GPI inositol-deacylase PGAP1-like alpha/beta" evidence="2">
        <location>
            <begin position="80"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU365011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9685171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1071" mass="118927" checksum="A50F24F48FB470AB" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZBL4</accession>
    <name>A0AA38ZBL4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_017807</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZBL4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050907">
        <property type="entry name" value="SRSF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23147">
        <property type="entry name" value="SERINE/ARGININE RICH SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="36"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="120"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="14"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="154"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="174"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9685879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="184" mass="21044" checksum="BC912948CB04CDA6" modified="2024-01-24" version="1">MRGRSYSPSPPRGYSRRGRSPSPRGRHGGRGRDLPTSLLVRNLRHDCRPEDLRRPFGQFGPLKDIYLPRDYYTGEPRGFGFVQYVDPADAAEAKYQMDGQVLLGRELTVVFAEENRKRPVDMRARERTRSVSPQDKRRYSGEQPYSASPPVKGSKSQSRSPSRSRSQTPVDYSRGLDRERSLSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZCJ6</accession>
    <name>A0AA38ZCJ6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_015428</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZCJ6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015149">
        <property type="term" value="F:hexose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17315">
        <property type="entry name" value="MFS_GLUT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045263">
        <property type="entry name" value="GLUT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503:SF103">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="131"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="188"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="221"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="253"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="333"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="368"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="398"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="430"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="465"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="497"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="95"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9686521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="539" mass="59063" checksum="1B7DEFF99F6A087A" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZDB2</accession>
    <name>A0AA38ZDB2_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_015676</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZDB2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9686916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="110" mass="12763" checksum="F67F6DA0547700B5" modified="2024-01-24" version="1">MEIHKFPHPSKLSLIMFAVTDLNKTSHIQGHLQKTNQKDDGWMNLQKGLELYRIIFMTKTMGSIPSMPLQFNQTASSMRKLKLTLKGFSKKKPYYLSSRRGWLLPPNVKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZG61</accession>
    <name>A0AA38ZG61_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Pectinesterase</fullName>
            <ecNumber evidence="4 12">3.1.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_013861</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Acts in the modification of cell walls via demethylesterification of cell wall pectin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 12">
            <text>[(1-&gt;4)-alpha-D-galacturonosyl methyl ester](n) + n H2O = [(1-&gt;4)-alpha-D-galacturonosyl](n) + n methanol + n H(+)</text>
            <dbReference type="Rhea" id="RHEA:22380"/>
            <dbReference type="Rhea" id="RHEA-COMP:14570"/>
            <dbReference type="Rhea" id="RHEA-COMP:14573"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17790"/>
            <dbReference type="ChEBI" id="CHEBI:140522"/>
            <dbReference type="ChEBI" id="CHEBI:140523"/>
            <dbReference type="EC" id="3.1.1.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 12">Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the pectinesterase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the PMEI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.11" evidence="4 12"/>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9687823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZG61"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004857">
        <property type="term" value="F:enzyme inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030599">
        <property type="term" value="F:pectinesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042545">
        <property type="term" value="P:cell wall modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045490">
        <property type="term" value="P:pectin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd15798">
        <property type="entry name" value="PMEI-like_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.40:FF:000001">
        <property type="entry name" value="Pectinesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000001">
        <property type="entry name" value="Pectinesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.40">
        <property type="entry name" value="Invertase/pectin methylesterase inhibitor family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035513">
        <property type="entry name" value="Invertase/methylesterase_inhib"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033131">
        <property type="entry name" value="Pectinesterase_Asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000070">
        <property type="entry name" value="Pectinesterase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006501">
        <property type="entry name" value="Pectinesterase_inhib_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01614">
        <property type="entry name" value="PME_inhib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31707">
        <property type="entry name" value="PECTINESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01095">
        <property type="entry name" value="Pectinesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04043">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00856">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101148">
        <property type="entry name" value="Plant invertase/pectin methylesterase inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00503">
        <property type="entry name" value="PECTINESTERASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0063">Aspartyl esterase</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="Pectinesterase inhibitor" evidence="15">
        <location>
            <begin position="79"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="51"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00057335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9687823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="599" mass="66019" checksum="35C50FC7D50F5555" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZG89</accession>
    <name>A0AA38ZG89_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_014265</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007032">
        <property type="term" value="P:endosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006898">
        <property type="term" value="P:receptor-mediated endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000641">
        <property type="term" value="P:regulation of early endosome to late endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000030">
        <property type="entry name" value="DnaJ homolog subfamily C GRV2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000180">
        <property type="entry name" value="DnaJ homolog subfamily C GRV2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000553">
        <property type="entry name" value="dnaJ homolog subfamily C GRV2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044978">
        <property type="entry name" value="GRV2/DNAJC13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045802">
        <property type="entry name" value="GRV2/DNAJC13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025640">
        <property type="entry name" value="GYF_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36983">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36983:SF2">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14237">
        <property type="entry name" value="GYF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19432">
        <property type="entry name" value="RME-8_N"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="1558"/>
            <end position="1640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="791"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2378"/>
            <end position="2404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2583"/>
            <end position="2609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="11"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="801"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2395"/>
            <end position="2404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2592"/>
            <end position="2601"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9688515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="2609" mass="284324" checksum="EFA9A9A28A6ACCE7" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZGN0</accession>
    <name>A0AA38ZGN0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">TBC1 domain family member 15</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">GTPase-activating protein RAB7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_014384</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Acts as a GTPase activating protein for RAB7A. Does not act on RAB4, RAB5 or RAB6.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Interacts with non-phosphorylated form of RAB8A; phosphorylation of RAB8A at 'Thr-72' disrupts this interaction. Interacts with ARMC12.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZGN0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000005">
        <property type="entry name" value="TBC1 domain family member 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000005">
        <property type="entry name" value="TBC1 domain family member 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021935">
        <property type="entry name" value="SGSM1/2_RBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957:SF502">
        <property type="entry name" value="SMALL G PROTEIN SIGNALING MODULATOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12068">
        <property type="entry name" value="PH_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="11">
        <location>
            <begin position="385"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="194"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="269"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="195"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="290"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00055283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00065268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00067480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00082539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9688710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="684" mass="78315" checksum="D19A1757224B94F8" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZJ14</accession>
    <name>A0AA38ZJ14_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Senescence regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_012438</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the senescence regulator S40 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9689750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZJ14"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010150">
        <property type="term" value="P:leaf senescence"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007608">
        <property type="entry name" value="Senescence_reg_S40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46525">
        <property type="entry name" value="EMB|CAB72159.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46525:SF18">
        <property type="entry name" value="SENESCENCE REGULATOR S40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04520">
        <property type="entry name" value="Senescence_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00034773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9689750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="209" mass="22996" checksum="3640A70D9F06EA29" modified="2024-01-24" version="1">MAKGRKLTTSRSERLLGNFAYGHGQGTGAESSELGEEDVWSMVERDDHVNGGGGGGGQSDWSPRAGGEPPMRNHHHRRRIPRDDRHQVGGLSLAFDDSGKTTSSRIVHQYRPQDGVTPSPRGHHMATSAPVNVPDWHKILRVDSVESLHDSDDDRELEMVPPHEYLAREYAASRKMAATSVLEGVGRTLKGRDLSRVRDAVWSQTGFDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZMF8</accession>
    <name>A0AA38ZMF8_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_013473</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZMF8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="191"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="209"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9691302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="241" mass="26574" checksum="1D00955A6FB1E666" modified="2024-01-24" version="1">MAALFYFEDKVHLKQLQRAKKYPLFFPRMLGTILEYYGFPTESDKEKKHHCREVYTVSRWQGSVTKTESTLTSASLTTANEHHYVPPPNLILEDFGDGEDTPPRPPAATTPGATSTPQNPDSAAHILASLSSTPNIGPSASDGYVHISAEAFNQLLARLDMIQENQANIQLTQRQLVQRVDELTMAVQQWNSSSEKPASFHDAPPLSVPPTQETNSTTKESTVPPTILTPSEITPSHPSHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZS39</accession>
    <name>A0AA38ZS39_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_009990</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZS39"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05476">
        <property type="entry name" value="pepsin_A_like_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034161">
        <property type="entry name" value="Pepsin-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051708">
        <property type="entry name" value="Plant_Aspart_Prot_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032799">
        <property type="entry name" value="TAXi_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032861">
        <property type="entry name" value="TAXi_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47967:SF14">
        <property type="entry name" value="EUKARYOTIC ASPARTYL PROTEASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47967">
        <property type="entry name" value="OS07G0603500 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14541">
        <property type="entry name" value="TAXi_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14543">
        <property type="entry name" value="TAXi_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041372063" description="Peptidase A1 domain-containing protein" evidence="6">
        <location>
            <begin position="24"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="7">
        <location>
            <begin position="93"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9694275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="441" mass="48350" checksum="23A4BEDC5163D21D" modified="2024-01-24" version="1">MASVNNLLLIICFTFIFSPCISAASDSKGFSTNLFHIHSPSSPYKNAKAESLAEDTALKSTLSRHAYIRARQQKALQPADFVPPPPIPDRDMFLANLSIGNPPTNVYVVLDTGSDLFWIQCEPCNVCYKQKDPIYNRTKSYSYTEMTCDDSLCLSLGREGQCSDSGSCRYECAYADGSRTSGLLSYDKVAFTSHYSTEDKTAQVGFGCGLQNLNIITGYRDGGVLGLGPGQVSLVSQLSSIGKVSKSFAYCFGNVSDPNAGGFLVFGDDTYLNGDMTPMVIAEFYYINLLGIGLGLGEPRLDINSSSFERKPDGSGGVIIDSGSTLSVFVPEVYEVVRNAVVDKLRKGYNISPLEQTPNCFIGKIGRDLEFFPTMVLYLESTGILNDEWSIFLQRYDDLFCLGFTRGYGLNIIGTLAQQSYKFGFNLEQNTLSIESKPNCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZU74</accession>
    <name>A0AA38ZU74_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Matrin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_010679</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SF3A2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZU74"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000245">
        <property type="term" value="P:spliceosomal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.2690:FF:000002">
        <property type="entry name" value="Splicing factor 3a subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2690">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000690">
        <property type="entry name" value="Matrin/U1-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052092">
        <property type="entry name" value="SF3A2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031781">
        <property type="entry name" value="SF3A2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23205">
        <property type="entry name" value="SPLICING FACTOR 3A SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23205:SF0">
        <property type="entry name" value="SPLICING FACTOR 3A SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16835">
        <property type="entry name" value="SF3A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01050">
        <property type="entry name" value="CactinC_cactus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50171">
        <property type="entry name" value="ZF_MATRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Matrin-type" evidence="11">
        <location>
            <begin position="52"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="213"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="222"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="233"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="266"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="330"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9695311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="357" mass="39371" checksum="579F29976D456083" modified="2024-01-24" version="1">MDRDWGSKPGSGGAATAQNEAIDRRERLRRLALETIDLAKDPYFMRNHLGSYECKLCLTLHNNEGNYLAHTQGKRHQTNLAKRAAREAKEAPAQPQPHKRKVSVRKTVKIGRPGYRVTKQFDPETKQRSLLFQIEYPEIEDLAKPRHRFMSSFEQRVQSFDKRYQYLMFAAEPYEIIAFKVPSTEIDKSTPKFFSHWDPDSKMFTLQLYFKTKPPEANKPQPASAANGTTAPGAPPRPLPPPPQAPPPPPPPPQGLPPGATIANPPRGPPPPMPGTLPPPPPPMGNGPRPMPPGGALPVPPPPVGSGTMANFTPGTQMARPPSMMPPQGFPGQQMQGQGIRPPMQPPNMGQQVPRPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZUJ1</accession>
    <name>A0AA38ZUJ1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Calcineurin-like phosphoesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_010784</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZUJ1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07383">
        <property type="entry name" value="MPP_Dcr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011230">
        <property type="entry name" value="PAP14/16/28/29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32440:SF11">
        <property type="entry name" value="METALLOPHOSPHOESTERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32440">
        <property type="entry name" value="PHOSPHATASE DCR2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF030250">
        <property type="entry name" value="Ptase_At2g46880"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041373054" description="Calcineurin-like phosphoesterase domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="2">
        <location>
            <begin position="42"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9695477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="375" mass="41834" checksum="53B239C24F224036" modified="2024-01-24" version="1">MSLTSLYLLYPLILTIGFGSSSEEHDALPLNYLQVRPGSSSFKIALFADLHFGESAWIDWGPLQDVNSTKVMSVVLDQETPDFVVYLGDVITANNIAIGNASLYWEEAISPTRARGIPWASVFGNHDDAPFEWPLEWFSATGIPHTHCTLPNSSGSGEQECSFRGTRRIELMKNEIKQNNLSYSRNGPKDLWPSISNFVLQVSSSQDPDSAVALLYFLDSGGGSYPEVISSSQAEWFNRKSQELNPSSRVPEMIFWHIPSKAYKKVAPKLGIHKPCVGSINREGVATQEAEMGIMKLLVKRPSVKAVFVGHNHGLDWCCPYEKLWLCFARHTGYGGYGNWARGTRILEITQQPFSLKSWIRMEDGQLHSEVVLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZUZ8</accession>
    <name>A0AA38ZUZ8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_010953</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZUZ8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033612">
        <property type="term" value="F:receptor serine/threonine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000516">
        <property type="entry name" value="Leucine-rich repeat family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000388">
        <property type="entry name" value="Leucine-rich repeat receptor-like tyrosine-protein kinase PXC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000652">
        <property type="entry name" value="probably inactive leucine-rich repeat receptor-like protein kinase At5g06940"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000534">
        <property type="entry name" value="Probably inactive leucine-rich repeat receptor-like protein kinase At5g06940"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050647">
        <property type="entry name" value="Plant_LRR-RLKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056:SF31">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041267174" description="Protein kinase domain-containing protein" evidence="14">
        <location>
            <begin position="24"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="539"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="16">
        <location>
            <begin position="24"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="Serine-threonine/tyrosine-protein kinase catalytic" evidence="15">
        <location>
            <begin position="600"/>
            <end position="862"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF07714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9695731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="19">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="891" mass="96929" checksum="AD27909557C2B29C" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZV62</accession>
    <name>A0AA38ZV62_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_010396</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZV62"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008004">
        <property type="entry name" value="OCTOPUS-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31659">
        <property type="entry name" value="PROTEIN: UPF0503-LIKE PROTEIN, PUTATIVE (DUF740)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31659:SF9">
        <property type="entry name" value="PROTEIN: UPF0503-LIKE PROTEIN, PUTATIVE (DUF740)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05340">
        <property type="entry name" value="DUF740"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="372"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="573"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="195"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="580"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="602"/>
            <end position="611"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9694903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="660" mass="74115" checksum="4F814CEF761B102F" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZWG4</accession>
    <name>A0AA38ZWG4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Exopolygalacturonase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_008665</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the glycosyl hydrolase 28 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZWG4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004650">
        <property type="term" value="F:polygalacturonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000004">
        <property type="entry name" value="Pectin lyase-like superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000743">
        <property type="entry name" value="Glyco_hydro_28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31375">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00295">
        <property type="entry name" value="Glyco_hydro_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00502">
        <property type="entry name" value="POLYGALACTURONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0134">Cell wall</keyword>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6 9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <feature type="active site" evidence="8">
        <location>
            <position position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9696558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="463" mass="50073" checksum="A18AD650146A8D7F" modified="2024-01-24" version="1">MRDDVIGTWKMANWANKTRDGNDGLWVRASNAFTATLGCSSNSTIKVTMAMAIRFVAKAVILLGLALISCNAQGDPLRDDLRRLLADTGATVFDVTKYGAVADGKTDNVEAFMKTWIAACHWRGTSTFLVPEGKFLVGQATFEGPCNSPNPIVFEAQGTVLAQPDLSEYPSPEWLMFEDVNQLIVKGGGTFDGQGVFAWKYNDCHQNADCQMLPTNLKFNKVNNSVVQDITSLDSKSFHFFVTNSHNFTAFNLTITAPGNSPNTDGMHLSRCDLVNLSNFTIKTGDDCISVGQGNTNINITDVYCGPGHGLSIGSLGKYPNEKDVVGIHVTNCTLNGTTNGARIKTWPGSPPSQASSIIFQDIVMNSVKNPIVIDQKYGAHTGKPSRVKVSNVLYKNLRGTTISKVAVSLVCSELVPCNGLQLEDINFTYNGPATFKRNLPFSSTCLHANVSYVGKQNPPTCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZZ31</accession>
    <name>A0AA38ZZ31_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cytochrome c-type biogenesis protein CcmE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_009332</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZZ31"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017004">
        <property type="term" value="P:cytochrome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017003">
        <property type="term" value="P:protein-heme linkage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01959">
        <property type="entry name" value="CcmE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004329">
        <property type="entry name" value="CcmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036127">
        <property type="entry name" value="CcmE-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34128">
        <property type="entry name" value="CYTOCHROME C-TYPE BIOGENESIS PROTEIN CCME HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34128:SF2">
        <property type="entry name" value="CYTOCHROME C-TYPE BIOGENESIS PROTEIN CCME HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03100">
        <property type="entry name" value="CcmE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82093">
        <property type="entry name" value="Heme chaperone CcmE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0201">Cytochrome c-type biogenesis</keyword>
    <keyword evidence="2" id="KW-0349">Heme</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9697467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="258" mass="28764" checksum="16D2BBEFBD9BC119" modified="2024-01-24" version="1">MAARLGLHLRSHLLRSAVLFQSSLRRQPPVFAVIESPASTLSNARVIDYPFTASGLRFFSTGGRRRPVRSKNIDIGARARQIQNRRLWTYSLSIGSASALIVLVLYQFQDQMVFYVTPTDALEDYAKNPSKNKFRLGGLVLEGSVKQPVSSPDMEFVVTDLITDILVRYDGALPDLFREGHSVVVEGFVKPFTDEIRKDESARSVAGKARSMDCYFSAIEVLAKHDEKYMPQEVAAAIERNKKKLAEMAEEGETATSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A094</accession>
    <name>A0AA39A094_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">VOC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_007450</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A094"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07264">
        <property type="entry name" value="VOC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="entry name" value="2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050383">
        <property type="entry name" value="GlyoxalaseI/FosfomycinResist"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC_core"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21366">
        <property type="entry name" value="GLYOXALASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21366:SF22">
        <property type="entry name" value="VOC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="8"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9698374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="144" mass="16134" checksum="4337C5E55A03B69F" modified="2024-01-24" version="1">MASNLSPVFAYVVLYVKDVAKSTAFYAKAFGCNVRRLDESHRWGELESGKTTIAFTPVHQRETDRITGAVQDPWSGRERPPVELCFAYVDVDAAYKRAVENGAVAVSEPEEKEWGQKVGYVRDIDGIVVRMGSHVNPPKQHHQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A0J4</accession>
    <name>A0AA39A0J4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">anthranilate synthase</fullName>
            <ecNumber evidence="4">4.1.3.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_007619</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterotetramer consisting of two non-identical subunits: a beta subunit and a large alpha subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the anthranilate synthase component I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.27" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A0J4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004049">
        <property type="term" value="F:anthranilate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.120.10:FF:000003">
        <property type="entry name" value="Anthranilate synthase component 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.120.10">
        <property type="entry name" value="Anthranilate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005801">
        <property type="entry name" value="ADC_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019999">
        <property type="entry name" value="Anth_synth_I-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006805">
        <property type="entry name" value="Anth_synth_I_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005256">
        <property type="entry name" value="Anth_synth_I_PabB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015890">
        <property type="entry name" value="Chorismate_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00564">
        <property type="entry name" value="trpE_most"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11236">
        <property type="entry name" value="AMINOBENZOATE/ANTHRANILATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11236:SF9">
        <property type="entry name" value="ANTHRANILATE SYNTHASE COMPONENT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04715">
        <property type="entry name" value="Anth_synt_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00425">
        <property type="entry name" value="Chorismate_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00095">
        <property type="entry name" value="ANTSNTHASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56322">
        <property type="entry name" value="ADC synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Anthranilate synthase component I N-terminal" evidence="10">
        <location>
            <begin position="104"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="Chorismate-utilising enzyme C-terminal" evidence="9">
        <location>
            <begin position="320"/>
            <end position="590"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9698641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="608" mass="67608" checksum="1D86540F73CB26B5" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A0N6</accession>
    <name>A0AA39A0N6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">6,7-dimethyl-8-ribityllumazine synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_007739</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A0N6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025322">
        <property type="entry name" value="PADRE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33052">
        <property type="entry name" value="DUF4228 DOMAIN PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14009">
        <property type="entry name" value="PADRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041408887" description="6,7-dimethyl-8-ribityllumazine synthase" evidence="2">
        <location>
            <begin position="18"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9698808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="148" mass="16030" checksum="BE78387B9485F0DE" modified="2024-01-24" version="1">MGNSLRCCLACVLPCGALDLIRIVHLNGHMEEIAGPVTAGEILQAHPNHVLSKPCSQGVVRKILILSPESELKRGSIYFLIPATSLPEKKRSGSGSNPKKSSKKSSRKCNSDVGSTEMISEKKSSGRDRRSSGRVGVWRPHLESILED</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A0R7</accession>
    <name>A0AA39A0R7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NOT2/NOT3/NOT5 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_007407</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CNOT2/3/5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A0R7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030015">
        <property type="term" value="C:CCR4-NOT core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.1020:FF:000004">
        <property type="entry name" value="probable NOT transcription complex subunit VIP2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1020">
        <property type="entry name" value="CCR4-NOT complex subunit 2/3/5, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038635">
        <property type="entry name" value="CCR4-NOT_su2/3/5_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040168">
        <property type="entry name" value="Not2/3/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007282">
        <property type="entry name" value="NOT2/3/5_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23326">
        <property type="entry name" value="CCR4 NOT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04153">
        <property type="entry name" value="NOT2_3_5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NOT2/NOT3/NOT5 C-terminal" evidence="5">
        <location>
            <begin position="534"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="59"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="265"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="368"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="417"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="65"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="265"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="287"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="377"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="417"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9698307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="666" mass="70840" checksum="7714BE561A96915F" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A0Z4</accession>
    <name>A0AA39A0Z4_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_007699</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Tim17/Tim22/Tim23 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A0Z4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042721">
        <property type="term" value="C:TIM22 mitochondrial import inner membrane insertion complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030943">
        <property type="term" value="F:mitochondrion targeting sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039175">
        <property type="entry name" value="TIM22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14110">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14110:SF0">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02466">
        <property type="entry name" value="Tim17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9698764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="170" mass="17960" checksum="4E65DD7D805F2030" modified="2024-01-24" version="1">MSSNSGSEFTNDSSSSTEAEKPQIEPIRMPTVEEIRGQDIWNNCAVRSVASGVMGGGLGLLMGLFLGALDNPIMQEEMTGRQQFIYTAKQMGRRSWGSAKTFAVMGLIFSAAECVVEKARAKHDTTNTVVAGCVTGGAISAKGGPKAACVGCAGFATFSVLIEKFLDRHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A288</accession>
    <name>A0AA39A288_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_005470</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9699615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A288"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044550">
        <property type="term" value="P:secondary metabolite biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19124">
        <property type="entry name" value="AKR_AKR4A_4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000014">
        <property type="entry name" value="NAD(P)-linked oxidoreductase superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044497">
        <property type="entry name" value="AKR4A/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11732">
        <property type="entry name" value="ALDO/KETO REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00798">
        <property type="entry name" value="ALDOKETO_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00063">
        <property type="entry name" value="ALDOKETO_REDUCTASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="5">
        <location>
            <begin position="17"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="53"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="4">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9699615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="318" mass="35725" checksum="39EAEA45C4BC57DA" modified="2024-01-24" version="1">MEIPEVLLSSGQKMPLLGMGTATFPLPPPHQVTSILVDAIEVGYRHFDSAAVYASEEPLGRAVTEAIERGLIKSRSELFITSKLWCTDNHPDLVLPALKNTLQKLGMEYVDLYLVHWPVRLRKEAPIFEFPGEDVIPWDMKGTWAAMEECCRLGLARSIGVSNFSCKKLSQLLQHATIPPAVNQVEMNPAWHQVKLREFCEEKGIHVTAWSPLGANGAFWGSLAVVDNPILKEISTAKGKSLAQVALRWLHQQGVSIVVKSFNKERMQENLKIFDWELTDNELAKIKQILQHRGCPGQSFVHHEGPYKSLEDLWDDDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A3F5</accession>
    <name>A0AA39A3F5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RNA methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_005876</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class IV-like SAM-binding methyltransferase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A3F5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18086">
        <property type="entry name" value="HsC9orf114-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003750">
        <property type="entry name" value="Put_MeTrfase-C9orf114-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12150">
        <property type="entry name" value="CLASS IV SAM-BINDING METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12150:SF13">
        <property type="entry name" value="METHYLTRANSFERASE C9ORF114-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02598">
        <property type="entry name" value="Methyltrn_RNA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="32"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9700266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="362" mass="40395" checksum="A3BE29A639573E65" modified="2024-01-24" version="1">MGKKKRRSDFEAETENNTAENETELINGDSRSKKKKNKTHKDKYQATDIPTLTIAVPGSIIHNAQSLELATRLASQIARAATIFRIDEVVVFDCKSTSGDDSTGATPDASDENETGAAFLIRILRYLETPQYLRKILFPKHNSLKFVGMLPPLDAPHHLRKHEWGPYREGVTLKERAPSSVGTLVDVGLNKNVVIDQVLEPGIRVTVAMGTNRNLDADFVHQVVSSSKPREEVGTYWGYKVRYASNISSVFKECPFKGGYDHLIGTSEHGLIVKSSELTIPSFRHLLIAFGGLAGLEESVEEDHSLKGKNVREIFDSYLNTCPNQGSRTIRTEEAILISLQYFQEPINRALQRFEYQSQGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A5B9</accession>
    <name>A0AA39A5B9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Kinesin-like protein KIN-10A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_006181</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. KIN-10 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A5B9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000068">
        <property type="entry name" value="p-loop containing nucleoside triphosphate hydrolase superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24115:SF416">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIN-10A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24115">
        <property type="entry name" value="KINESIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0493">Microtubule</keyword>
    <keyword evidence="5 8" id="KW-0505">Motor protein</keyword>
    <keyword evidence="2 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="11">
        <location>
            <begin position="53"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="737"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="429"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="742"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="134"/>
            <end position="141"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00073419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9700737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="992" mass="110349" checksum="95039714BD0A1373" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A7E2</accession>
    <name>A0AA39A7E2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mitogen-activated protein kinase kinase kinase 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_004162</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A7E2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039903">
        <property type="entry name" value="Zswim2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21540:SF0">
        <property type="entry name" value="PHD FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21540">
        <property type="entry name" value="RING FINGER AND SWIM DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04434">
        <property type="entry name" value="SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="3">
        <location>
            <begin position="57"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="2">
        <location>
            <begin position="152"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9702287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="233" mass="26111" checksum="60F54E3D2DB13A59" modified="2024-01-24" version="1">MESVASNSSPSEHRLHPRFKPTQPVADRIFRAIRHRLRLLHRSESNFFVLGATGNVYTVTLSTNPSCTCPDRTTPCKHILFVFIKVLGVSLDDSCLRRRTLRPCLLSHLLSKPTSPEAVAGAGVRERFHELFLQAREGSSRPAIEIEDGTMCPVCLDEMGRGEKVVACGTCRNPIHEECLLTWKRSRGRRSASCVICRARWSNRTDQERYLNLAAYVSDDDEVAEGDGGLWGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AAG2</accession>
    <name>A0AA39AAG2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_004502</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AAG2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05472">
        <property type="entry name" value="cnd41_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000013">
        <property type="entry name" value="Aspartyl protease AED1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000021">
        <property type="entry name" value="Aspartyl protease AED1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033873">
        <property type="entry name" value="CND41-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032799">
        <property type="entry name" value="TAXi_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032861">
        <property type="entry name" value="TAXi_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13683:SF750">
        <property type="entry name" value="ASPARTYL PROTEASE AED1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13683">
        <property type="entry name" value="ASPARTYL PROTEASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14541">
        <property type="entry name" value="TAXi_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14543">
        <property type="entry name" value="TAXi_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041323017" description="Peptidase A1 domain-containing protein" evidence="8">
        <location>
            <begin position="26"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="9">
        <location>
            <begin position="139"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="157"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9702794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="482" mass="51148" checksum="8A6B6C68666B731E" modified="2024-01-24" version="1">MATAISTIFLLKFLLYAALLSSKRGLAFQGRKTALSTSSTLHNVQVTCLMPSSVCSPSSPKGDDKRASLEVIHKHGPCSKLSQDKASSLFHTQMLDQDESRVNSIRSRLAKNPADGGKLKGSKATLPSKSGRTIGTGNYVVTVGLGTPKRDLTLVFDTGSDLIWTQCEPCARYCYHQQEPIFNPSKSTSYTNISCSSPTCNELQSGTGNSPSCSASTCVYGIQYGDQSYSVGFFAQDKLTLTPTDVFNNFLFGCGQNNRGLFVGAAGLLGLGRNALSLVSQTAEKYGKLFSYCLPSTSSSTGYLTFGSGGGTSKAVKFTPSLVNSQGPSFYFLNLIAISVGGRKLSTSASVFSTAGTIIDSGTVISRLPPTAYSDLRAAFQQQMSKYPKAAPASILDTCYNFSQYDTVDVPKINLYFSDGAEMDLDPSGIFYVLSISQVCLAFAGNSDATDIAILGNVQQKTFNVVYDVAGGRIGFGPGGCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AAX5</accession>
    <name>A0AA39AAX5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_005418</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9704116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AAX5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47936:SF1">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN GUN1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47936">
        <property type="entry name" value="PPR_LONG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="116"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="221"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="301"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="336"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="407"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="442"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9704116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="525" mass="60801" checksum="BA200EECFF85BDA8" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ABD3</accession>
    <name>A0AA39ABD3_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_004876</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ABD3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007877">
        <property type="entry name" value="DUF707"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31210:SF74">
        <property type="entry name" value="LYSINE KETOGLUTARATE REDUCTASE TRANS-SPLICING-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31210">
        <property type="entry name" value="OS06G0731900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05212">
        <property type="entry name" value="DUF707"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="328"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9703259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="413" mass="47117" checksum="C147D21811923D94" modified="2024-01-24" version="1">MKLPNCVSQLADSKSRRSCVCSIFPTASVLCLMFFIGSVLIGQDYSEKLSRWGMSTGMLNSVSNKCENQCRANGSEALPKGIVVTSSDLDMRPLWGFPKKRKDPKRNLLTVAVGVKQKDLVNKMVEKFLSDGFVVMLFHYDGVVDEWKDFKWCDRVLHVAAINQTKWWFAKRFLHPEIVAEYNYIFLWDEDLGVTDFNPKRYVATVEREGLEISQPALDGSKSEVHHQITLRGRRSDVHRRIFKSGGSGKICDKNSTAPPCTGWIEVMAPVFSREAWRCVWYMIQNDLIHAWGLDMQLGYCAQGDRTKNVGVVDSDYIVHYGLPTLGANDPDKTTSPVQDDDSEPEKITTTTTAEPPIPKLPASSTSPINFRVEVRRQSYIEYNIFKKRWRRAVVEDKCWKDPYQQLGEKNTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ACN1</accession>
    <name>A0AA39ACN1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Strictosidine synthase conserved region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_005239</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the strictosidine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ACN1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009753">
        <property type="term" value="P:response to jasmonic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000073">
        <property type="entry name" value="Protein STRICTOSIDINE SYNTHASE-LIKE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018119">
        <property type="entry name" value="Strictosidine_synth_cons-reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10426:SF88">
        <property type="entry name" value="ADIPOCYTE PLASMA MEMBRANE-ASSOCIATED PROTEIN HEMOMUCIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10426">
        <property type="entry name" value="STRICTOSIDINE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20067">
        <property type="entry name" value="SSL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03088">
        <property type="entry name" value="Str_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63829">
        <property type="entry name" value="Calcium-dependent phosphotriesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0926">Vacuole</keyword>
    <feature type="domain" description="Strictosidine synthase conserved region" evidence="7">
        <location>
            <begin position="171"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9703874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="383" mass="42696" checksum="BCE60CCE349E27E4" modified="2024-01-24" version="1">MAKSKPSDTNITRKSSSWSLGLLISILTPVAAAIFLYQLDSFDPASLPTHVFSQEPMSVPRINSRLLQESEMIGLGKLLGPEDIAYDTNSHLIYTGCADGWIKKVTLNDSVVNSVVHDWAFTGGRPLGVALGRAGEVLVADADKGLLEISEDGVVKLLTDEAEGIRFKLTDAVDVAVDGMIYFTDASYKYSLKDFIWDVLEGRPHGRLLSFDPSTQETKVLVRDLYFANGVVVSPDQTFLIFCETFMKRCSKYYIEGERKGFVDKFIDNLPGMPDNILYDGEGYYWIGLATGYNYLWDLALKYPSIRKVMAILERIIGRPEIEKNGGVLAIDLEGKPVAHYYDPTLSLVSSGIKIGKYLYCGFVVRPYMIRLDLDHHDARTTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ADY8</accession>
    <name>A0AA39ADY8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Trichome birefringence-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_003459</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ADY8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016413">
        <property type="term" value="F:O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029962">
        <property type="entry name" value="TBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025846">
        <property type="entry name" value="TBL_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285:SF12">
        <property type="entry name" value="PROTEIN TRICHOME BIREFRINGENCE-LIKE 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285">
        <property type="entry name" value="PROTEIN TRICHOME BIREFRINGENCE-LIKE 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14416">
        <property type="entry name" value="PMR5N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="Trichome birefringence-like N-terminal" evidence="2">
        <location>
            <begin position="73"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9705424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="342" mass="38788" checksum="1FC9C3590F0C189C" modified="2024-01-24" version="1">MPIRGHCFHHVKKNASHSSSCSTSLLFISLLCLASLFLVDSLFKSYVSPRNHQNLQFKRSDSSGTPILVEPSDYSDGMWIYDPSVRSAPYDHTCKEIFKGWNCIAGNKSNGLDIIKWRWKPKGCDLPRFDSVRLLERFRNTNVGWALIDPSLSLSLSLSYTHVKSLFLSLFMLLTCLSQEWSADANGGTLESLGYKEGFRVDVDIPDGTWAEAPSFPDILIFNTGHCMNLAWWDRNNMLGVLPKLDLIFNDTQYFFKISCINIDIFISNPIMHFDSNDLSTKITPHIYSLFTKVNDKSYYLVCVVFINTLKGLSFHPITNSIISNVGSRSKWPQDGQTLKIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AGS0</accession>
    <name>A0AA39AGS0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_002370</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AGS0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11073">
        <property type="entry name" value="CYP76-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000007">
        <property type="entry name" value="Cytochrome P450 76C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47950">
        <property type="entry name" value="CYTOCHROME P450, FAMILY 76, SUBFAMILY C, POLYPEPTIDE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47950:SF44">
        <property type="entry name" value="CYTOCHROME P450, FAMILY 76, SUBFAMILY C, POLYPEPTIDE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0349">Heme</keyword>
    <keyword evidence="4 5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="2 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041376580" description="Cytochrome P450" evidence="7">
        <location>
            <begin position="29"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="5">
        <location>
            <position position="436"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9707343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="495" mass="55807" checksum="B7188A9DC60AB24D" modified="2024-01-24" version="1">MELNTFLLLCMPLILYFFLQFLRPSSHATKLPPGPTGLPILGSLLKIGNLPHRSLARLAKIHGPLITLRLGSITTVVATSPQTAKLILQTHAQNFLDRPVPEAIDSPQGTIGWIPGDHVWRSRRRVVINHLFTSQSLDSLQHLRYKKVEQLLQHIRKHCVSGTPVDIGLLTSAISLNVLSNAIFSVDLVDPGFESAQDFRDQVWGIMEGAGKFNISDYFPMFRRFDLLGVKRDTFSSYKRLYEIVGDLIKSRIKCRASNPMSRNDDFLDVILDQCQEDGSYFNSDNIQVLIVELFYAGSDTSTITTEWAMTELLRNPRVMQKVRQELSEVIGAGQMVRESDMDRLPYFQAVVKETLRLHPAGPLLLPFKAKNDVELCGFTIPSNSHVLVNMWAIARDPSYWEDPSSFLPERFLGSKIDYRGQDYEYIPFGAGRRICPGIPLAIRMVQLVLASIIHSFNWKLPEGTTPLTIDMQEKCGATLKKAIPLSAIPFIEEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AGX6</accession>
    <name>A0AA39AGX6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NmrA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_002453</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NmrA-type oxidoreductase family. Isoflavone reductase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AGX6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010283">
        <property type="term" value="F:pinoresinol reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009807">
        <property type="term" value="P:lignan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05259">
        <property type="entry name" value="PCBER_SDR_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008030">
        <property type="entry name" value="NmrA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050608">
        <property type="entry name" value="NmrA-type/Isoflavone_red_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045312">
        <property type="entry name" value="PCBER-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43349:SF4">
        <property type="entry name" value="PINORESINOL REDUCTASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43349">
        <property type="entry name" value="PINORESINOL REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05368">
        <property type="entry name" value="NmrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NmrA-like" evidence="4">
        <location>
            <begin position="3"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9707436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="312" mass="34991" checksum="628D065489C3FAEE" modified="2024-01-24" version="1">MDKSKVLVVGGTGYIGRRMVEASLAQGHPTFVLQRPEIGMDIEKLQMLLSFKAKGATLVEGSFADHKSLVEAVKKVDVVICTMSGVHFRSHNLLIQLKLVEAIKEAGNIKRFLPSEFGMDPARMGDALEPGRVTFDEKMVVRKAIEEANIPHTYVSSNCFAAYFVPNCSQLGTLTPPKEKVSIYGDGNIKAIFVDEDDVAAYTIKAIDDPRTLNKTVYIRPPENILSQRQIIEMWEKLTGKKLDKSSISAEDFLASMKGLDYASQVGVGHFYHIYYEGCLTNFEIGEEGEEASKLYPEVRYIRMDEYLKRYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AHE0</accession>
    <name>A0AA39AHE0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UBX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_001704</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AHE0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01767">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001012">
        <property type="entry name" value="UBX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050730">
        <property type="entry name" value="UBX_domain-protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23322:SF1">
        <property type="entry name" value="FAS-ASSOCIATED FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23322">
        <property type="entry name" value="FAS-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00789">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00166">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50033">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBX" evidence="2">
        <location>
            <begin position="15"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9706304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="96" mass="10723" checksum="208A8561133E4F8A" modified="2024-01-24" version="1">MRQEKALSLGSEPEKGPNVTQVLVRFPSGERKDRRFHSTATIQSLYDYVDSLGCLDAESYSLVSNFPRVVYGPEKLSLSLKEAGLHPQASLFVELH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AI75</accession>
    <name>A0AA39AI75_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">4-coumarate--CoA ligase</fullName>
            <ecNumber evidence="2">6.2.1.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_002528</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(E)-4-coumarate + ATP + CoA = (E)-4-coumaroyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19641"/>
            <dbReference type="ChEBI" id="CHEBI:12876"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:85008"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.12"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:19642"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.12" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AI75"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016207">
        <property type="term" value="F:4-coumarate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05904">
        <property type="entry name" value="4CL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000007">
        <property type="entry name" value="4-coumarate--CoA ligase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000003">
        <property type="entry name" value="Long-chain-fatty-acid--CoA ligase FadD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096:SF149">
        <property type="entry name" value="AMP-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="108"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="268"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="9">
        <location>
            <begin position="68"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="10">
        <location>
            <begin position="482"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="8"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9707532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="571" mass="61702" checksum="B1BC2E103BE6DA14" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AK56</accession>
    <name>A0AA39AK56_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_000888</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AK56"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="105"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9709140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="230" mass="26562" checksum="DF364F9F605871BD" modified="2024-01-24" version="1">MLERMPCFTDPEPPPTKMSDFFPALKRLSTNIGGDPPIFVITQLPFGTLESAIARTEPLQDCTTREIAEVVDGVRNLIRRRDILLERLKVAKSMRAFISHRMSTTEELRARLEQVESELAATQKAADYGAKALKTAEVKKEATQRLRREREAMEGKCWEVEPENSRLKKEMEELRSGFATQKKDLEVEYQRQVDEMYFVGYRCYMKKNDITHNTPSFPFNDESEAPDDFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AKT0</accession>
    <name>A0AA39AKT0_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_000203</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AKT0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34197:SF3">
        <property type="entry name" value="DUF740 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34197">
        <property type="entry name" value="OS04G0591300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9708029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="382" mass="41938" checksum="CEB69636442FFE3C" modified="2024-01-24" version="1">MKERGKAVETYGGSCFADYYSTSDLPCRKHPSSSSVGICAYCLKDRLIKLVCSDCGEQRLSSCSCSEISSIPNSCTVEVGSVGRISFLIENERGEVSHSNSKHKSEEKSEQVVLLKRSSSSSVEIKKSRFWGIGRLFRKKRDKGFDDKSEGWMFDYMGVSRSRSLCSFRGGGYNESEDGDLAHSHARTSGVNGALAFDSDKKSGFSEEEARRSGFSCPERKDHDALDSKTGLDFRAVKKGSSLLELETRLKGAKRSGFPLKESDFSGLDDSGFIDLKLDFSSESKPDIFSLKMGALSESGSCFSSMRGGGHDQCGGCSFGKLIGDDDGAFSNGGTSCRITVNERGIKKGRKSLKVWRWILRNHPSWVSTTARKKNEDQILKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D3S5</accession>
    <name>A0AA39D3S5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_026569</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[DNA-directed RNA polymerase] + ATP = phospho-[DNA-directed RNA polymerase] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10216"/>
            <dbReference type="Rhea" id="RHEA-COMP:11321"/>
            <dbReference type="Rhea" id="RHEA-COMP:11322"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43176"/>
            <dbReference type="ChEBI" id="CHEBI:68546"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.23"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Interacts with CYCT1-3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D3S5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008353">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009615">
        <property type="term" value="P:response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07840">
        <property type="entry name" value="STKc_CDK9_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000273">
        <property type="entry name" value="Cyclin-dependent kinase C-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000272">
        <property type="entry name" value="Cyclin-dependent kinase C-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF560">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE C-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="25"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="335"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="335"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="425"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="454"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="504"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00065301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9670091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="513" mass="57475" checksum="1F418631772BF3D9" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D4I4</accession>
    <name>A0AA39D4I4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">NADPH-dependent diflavin oxidoreductase 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_025419</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D4I4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.80:FF:000032">
        <property type="entry name" value="NADPH-dependent diflavin oxidoreductase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003097">
        <property type="entry name" value="CysJ-like_FAD-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384:SF10">
        <property type="entry name" value="NADPH-DEPENDENT DIFLAVIN OXIDOREDUCTASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384">
        <property type="entry name" value="NITRIC OXIDE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00667">
        <property type="entry name" value="FAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sulfite reductase [NADPH] flavoprotein alpha-component-like FAD-binding" evidence="7">
        <location>
            <begin position="163"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="Oxidoreductase FAD/NAD(P)-binding" evidence="6">
        <location>
            <begin position="232"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9671693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="386" mass="42534" checksum="0C5643EF3226B3CA" modified="2024-01-24" version="1">MPYPSLCVHVNSEQGADMKGKQKQKLLVLDASQTRNALVAAERMPCSTSLHRLLQCCQGDTPDSMKASWDSVANIKKLEEPRCQCLGLQFVADKLDKRLLDLGAVAIVERGLGDDQHPSGGDVCLSWRLRFLVVLPFSLGGVGIRGFPLSANAIRVAVLADSYSISSSPLAHPNQVHLTVNVTPWTTLFKRKRMGLCSKWLTGLDPQQEVIIPAWFVKGSLPAPPPSLPLILIGRGTGCAPFRGFVEERAVQSTSCSTGPVLFFFGCWNEDDDFLYRDFWLSHSRNGGFGVLSEEKGGGLYVAFSREQQQKVYVQHKMREKSQRIWNLLCEGASIYVAGSSTKMPSDVFSSFEEIVSKENGVPRESAVRWLRAMVRAGRYHVGAGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D599</accession>
    <name>A0AA39D599_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">polynucleotide adenylyltransferase</fullName>
            <ecNumber evidence="3">2.7.7.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_025384</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA polymerase type-B-like family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.19" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D599"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031499">
        <property type="term" value="C:TRAMP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990817">
        <property type="term" value="F:poly(A) RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043634">
        <property type="term" value="P:polyadenylation-dependent ncRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031123">
        <property type="term" value="P:RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05402">
        <property type="entry name" value="NT_PAP_TUTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.460.10:FF:000006">
        <property type="entry name" value="non-canonical poly(A) RNA polymerase PAPD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1410.10:FF:000009">
        <property type="entry name" value="Poly(A) RNA polymerase cid14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054708">
        <property type="entry name" value="MTPAP-like_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002058">
        <property type="entry name" value="PAP_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045862">
        <property type="entry name" value="Trf4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23092:SF15">
        <property type="entry name" value="INACTIVE NON-CANONICAL POLY(A) RNA POLYMERASE PROTEIN TRF4-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23092">
        <property type="entry name" value="POLY(A) RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22600">
        <property type="entry name" value="MTPAP-like_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03828">
        <property type="entry name" value="PAP_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81631">
        <property type="entry name" value="PAP/OAS1 substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Poly(A) RNA polymerase mitochondrial-like central palm" evidence="9">
        <location>
            <begin position="105"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="PAP-associated" evidence="8">
        <location>
            <begin position="293"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="441"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="14"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="470"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="488"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF22600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9671651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="497" mass="55109" checksum="B2039E597C4248F2" modified="2024-01-24" version="1">MGTASYFYETLSPLSPPPSDRSPPPSDESQPYYVYRNQISLSSLSCPSPETAAPDYFSLDARADVEEPSPARFRTPPPASEEEAPAVESGWFRGNSGLRSPMLKLHKEILDFCDFLSPTPEEQSARNAAIESVFNVIRYIWPNCKVEVFGSFKTGLYLPTSDIDVVILGSDIKAPQIGLYALSRALSQKGIAKKIQVIAKARVPIIKFVEKRSSVAFDISFDVENGPKAAEYIQDAISKWPPLRPLCLILKVFLQQRELNEVYSGGIGSYALLAMLIAMLQNLQEWNASVEHNLGVLLVNFFDFYGRKLNTVDIGVTCNGPGTFFLKSTKGFVNKGQKFLISIEDPQLPGNDIGKNSFNYFQIRSAFSMAFSTLTNARTILGLDPNRSILGTIIRPDPVLLERKGGSDGTMTFDHLLPGAGEPLSPQTGGQELLCNWQVEDTEEEPLPRSNPIAGDGSAHSSGKKRKASKERLRNKVKEHADMEKDKSKKKRRWKHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D5J1</accession>
    <name>A0AA39D5J1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">SOSEKI DIX-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_026539</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="8">Homodimer. Forms long polymer filaments with other SOKs proteins polymers (e.g. SOK1, SOK2, SOK3 and SOK4) crucial for polar localization and biological activity. Binds to ANGUSTIFOLIA (AN).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the SOSEKI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D5J1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051258">
        <property type="term" value="P:protein polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051302">
        <property type="term" value="P:regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000067">
        <property type="term" value="P:regulation of root morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090708">
        <property type="term" value="P:specification of plant organ axis polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010369">
        <property type="entry name" value="SOK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048351">
        <property type="entry name" value="SOK_DIX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021182">
        <property type="entry name" value="SOK_magnoliopsida"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31083:SF18">
        <property type="entry name" value="PROTEIN SOSEKI 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31083">
        <property type="entry name" value="UPSTREAM OF FLC PROTEIN (DUF966)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06136">
        <property type="entry name" value="SOK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF031043">
        <property type="entry name" value="UCP031043"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SOSEKI DIX-like" evidence="10">
        <location>
            <begin position="31"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="139"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="359"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="144"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="157"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="170"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="185"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="224"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF06136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9670042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="409" mass="46308" checksum="3771B003C05318C4" modified="2024-01-24" version="1">MDIRARRSSRETSPDRAKVCLQPRVKPFRKVQVVYYLSRNGQLEHPHYMEVTHLANQQLRLKDVMERLTVLRGKGMPSLYSWSCKRSYKNGYVWNDLAENDIIYPAEGAEYVLKGSELIEGCTDKFQQLHVSNRVQHIPEPNFHPKRIPLPRRSRHREPVEVENMRDEEHDYQEEEEEEDEEKTSYTSSNTSRSRCSRGVSTDEIEATQKNSNPTELTLEDGSPPSTSSTVSDKANESNSNSKRFEDGDPVDPVFAEPVLSRNSVLLQLIACGSMVSGKPKNGTSLKRSSPNIPVKNTNLHKGVLCKTAAKVAEEDMINYISENPRFGNLQSEEKEYFSGSIVESMTEDRVSIQPVLKKSSSYNEERSSKAGLGEAVEEVEEKKEKAVKGKCIPRKKFSGTSSSKQSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D8A1</accession>
    <name>A0AA39D8A1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_023390</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Spinster (TC 2.A.1.49) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D8A1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17328">
        <property type="entry name" value="MFS_spinster_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000520">
        <property type="entry name" value="Major facilitator superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044770">
        <property type="entry name" value="MFS_spinster-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23505:SF72">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23505">
        <property type="entry name" value="SPINSTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="102"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="131"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="166"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="229"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="263"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="295"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="324"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="417"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="7"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9673810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="522" mass="56220" checksum="E27C3C2812756F06" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D9P0</accession>
    <name>A0AA39D9P0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Polysaccharide biosynthesis domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_024915</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9676151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D9P0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045492">
        <property type="term" value="P:xylan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006514">
        <property type="entry name" value="IRX15/GXM/AGM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01627">
        <property type="entry name" value="A_thal_3515"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31444">
        <property type="entry name" value="OS11G0490100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21729">
        <property type="entry name" value="IRX15_IRX15L_GXM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="45"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9676151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="303" mass="33958" checksum="69EF43338A255C66" modified="2024-01-24" version="1">MRPSKIQTSINLKLILICFLCLLLFLLVFKSNFTPFQENHPSPILETHVSNSTSTPAAEEQPPSRDCPSLPLTPTCTKTPPSLAQALVHYATLNITPQQTFSEISVSLRVLQKKAPCNFLVFGLGHDSLMWTALNHGGRTIFLEEDKSWIEQIKQKLPSLESYHVVYDSKVHQADELMEVGMGEECKVVVDPRFSKCKLSLKGFPNEVYDVEWDLIMVDAPTGYHDEAPGRMSAIYTAGLMARNREDGETDVFVHDVDRVVEDNFPRHFCVRATLGNKKAGLGVLPFQAIGLAQEDPFFAHEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DAR3</accession>
    <name>A0AA39DAR3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">polynucleotide adenylyltransferase</fullName>
            <ecNumber evidence="5">2.7.7.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PVL29_022089</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>RNA(n) + ATP = RNA(n)-3'-adenine ribonucleotide + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11332"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17347"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="ChEBI" id="CHEBI:173115"/>
            <dbReference type="EC" id="2.7.7.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the poly(A) polymerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.19" evidence="5"/>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9676914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DAR3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990817">
        <property type="term" value="F:poly(A) RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031123">
        <property type="term" value="P:RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05402">
        <property type="entry name" value="NT_PAP_TUTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.590:FF:000002">
        <property type="entry name" value="Nuclear poly(A) polymerase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.460.10:FF:000002">
        <property type="entry name" value="Poly(A) polymerase alpha, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1410.10:FF:000001">
        <property type="entry name" value="Putative poly(A) polymerase gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.590">
        <property type="entry name" value="Poly(A) polymerase predicted RNA binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011068">
        <property type="entry name" value="NuclTrfase_I-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007012">
        <property type="entry name" value="PolA_pol_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048840">
        <property type="entry name" value="PolA_pol_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007010">
        <property type="entry name" value="PolA_pol_RNA-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10682">
        <property type="entry name" value="POLY A POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10682:SF10">
        <property type="entry name" value="POLYNUCLEOTIDE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04928">
        <property type="entry name" value="PAP_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20750">
        <property type="entry name" value="PAP_NTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04926">
        <property type="entry name" value="PAP_RNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55003">
        <property type="entry name" value="PAP/Archaeal CCA-adding enzyme, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81631">
        <property type="entry name" value="PAP/OAS1 substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Poly(A) polymerase nucleotidyltransferase" evidence="18">
        <location>
            <begin position="17"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="Poly(A) polymerase central" evidence="17">
        <location>
            <begin position="216"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="Poly(A) polymerase RNA-binding" evidence="16">
        <location>
            <begin position="364"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="518"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="674"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="522"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="676"/>
            <end position="689"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF04926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF20750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ9676914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="757" mass="84915" checksum="1A3EB1F087C6E085" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DAW0</accession>
    <name>A0AA39DAW0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">F-box/kelch-repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_022390</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DAW0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000007">
        <property type="entry name" value="F-box/kelch-repeat protein SKIP11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052439">
        <property type="entry name" value="F-box/Kelch-repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46122:SF9">
        <property type="entry name" value="F-BOX_KELCH-REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46122">
        <property type="entry name" value="GALACTOSE OXIDASE/KELCH REPEAT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="39"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9677381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="443" mass="49048" checksum="135E6950E57FB442" modified="2024-01-24" version="1">MLEGPSYLISRDLPNACEQESKWIYNTFLVIELSNSKRPLEDGEDVGLRKLSKPSDGHETEETMQGFHDLSLSQANQSNTHHDTGDQSDLSSLISQIGRDNSINCLLQCSRSDYGSIASLNRSFRSLIRGGELYRLRRQLGIVEHWVYFSCNLLQWEAFDPIRRRWMHLPRMPSYECFMCSDKESLAVGTELLVFGKEVTSHVVYKYSILTNSWSSGMNMNSPRCLFGSASLGEIAILAGGCDPRGNILSSAELYNSDTGTWVTLPSMNKPRKMCSGIFMDRKFYVIGGIGVGNSNSLTCGEVYDLEMRTWREIPNMFPGRNGSAGVAEAAPAAAEAPPLVAVVNNELYAADYAEKEVRKYDKARNLWVTVGRLPEQAVSMNGWGLAFRACGDRLIVIGGPRVLGGGIIELNSWSPGEGPPQWDLLARKQSGSFVYNCAVMGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DBY7</accession>
    <name>A0AA39DBY7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_021345</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DBY7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000722">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046956">
        <property type="entry name" value="RLP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063">
        <property type="entry name" value="LRR RECEPTOR-LIKE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063:SF98">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE FLS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 14" id="KW-0732">Signal</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041263715" description="Leucine-rich repeat-containing N-terminal plant-type domain-containing protein" evidence="14">
        <location>
            <begin position="26"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="843"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="15">
        <location>
            <begin position="31"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="16">
        <location>
            <begin position="266"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9679388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="19">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="938" mass="104656" checksum="13118AFE3B9AA899" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DCQ8</accession>
    <name>A0AA39DCQ8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_020999</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DCQ8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895">
        <property type="entry name" value="CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895:SF28">
        <property type="entry name" value="CBL-INTERACTING SERINE_THREONINE-PROTEIN KINASE 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="25"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9678954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="144" mass="16752" checksum="5C5C633A9E73AE3C" modified="2024-01-24" version="1">MVQYMRDIFLFLADLSKLFSSEGVASPREHSWKGGLDKKYRNLKTGTSVAIKIIDKEKILKVGMVDQIKREIVMRLVRHPNVVELYEVMASKTKIYFVMEYAKGRELFNKVAKGNLKEDVARKHFQQLISAVDFCHSRGVYHRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DDN0</accession>
    <name>A0AA39DDN0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase M20 dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_021710</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="5">The Mn(2+) ion enhances activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DDN0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009850">
        <property type="term" value="P:auxin metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08017">
        <property type="entry name" value="M20_IAA_Hyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.360:FF:000001">
        <property type="entry name" value="N-acetyldiaminopimelate deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017439">
        <property type="entry name" value="Amidohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044757">
        <property type="entry name" value="ILR1-like_Hyd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01891">
        <property type="entry name" value="amidohydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11014:SF140">
        <property type="entry name" value="IAA-AMINO ACID HYDROLASE ILR1-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11014">
        <property type="entry name" value="PEPTIDASE M20 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005962">
        <property type="entry name" value="Pept_M20D_amidohydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 5" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041332490" description="Peptidase M20 dimerisation domain-containing protein" evidence="6">
        <location>
            <begin position="22"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="7">
        <location>
            <begin position="210"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="390"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR005962-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9679885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="425" mass="46156" checksum="C4A5F667AAF3EA73" modified="2024-01-24" version="1">MGCDSIAHVVLLLLLLGPCLCSREESSATQILSSAKKDREWLVSVRRKIHENPELRFEEYNTSALIRGELDKLGISYIHPLAKTGIVAEIGTGSGPVVALRADMDALPLQELVEWEHKSKIDGKMHGCGHDAHTTMLLGAAKLLSQRKHKLKGTVRLLFQPAEEGGLGAREMIKVGALGDAEVIFGMHIDHETPTGSIASRSGPLLAAVCSFEARIEGKGGDAAEPHTNADPILAASFSILALQQLISRELDPLDSQVLSVTSVKGGTTLNLTPSHVVLRGSLRSLTTEGLKQLQKRVKEVIEGQAAVHRCNAYFYRTEEDYLLPAVVNDEVMHQLVLRVGKLVLGPQNILVANKVMASEDFAFYQEVIPGVMFSIGIRNELVGSVHSPHSPHFFLDEDVLPIGAALHTALAEIYLDEHQNSTLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DE72</accession>
    <name>A0AA39DE72_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SHSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_021012</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DE72"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06464">
        <property type="entry name" value="ACD_sHsps-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044656">
        <property type="entry name" value="HSP14.7/HSP23.5/HSP23.6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46991">
        <property type="entry name" value="23.5 KDA HEAT SHOCK PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46991:SF11">
        <property type="entry name" value="SMALL HEAT SHOCK PROTEIN HSPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0346">Stress response</keyword>
    <keyword evidence="1" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="SHSP" evidence="6">
        <location>
            <begin position="98"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="141"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9678967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="208" mass="23730" checksum="F9C9B1A26AEC3292" modified="2024-01-24" version="1">MASSLALRSLLSKSLHPNFRALATAPSVSRSFNTNAIRDYDDDERRLDVDRLSDRSFSRRGDFAPSSFSDVFDPFSPTRSLSQVLNLMDHFMDNPFLSTSRGMGTGIRRSWDVKETDDALHLRVDMPGLSKEDVKVNVEQNTLTIQGEEKNETEDEESRRRYSSRIDLPEKLYKTGEIKAEMNNGVLKIVVPKLKEEERTDVINVKVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DEW4</accession>
    <name>A0AA39DEW4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_020176</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SINA (Seven in absentia) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9681160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DEW4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000041">
        <property type="entry name" value="E3 ubiquitin-protein ligase SINAT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.210.10:FF:000027">
        <property type="entry name" value="Pectinesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.210.10">
        <property type="entry name" value="Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018121">
        <property type="entry name" value="7-in-absentia-prot_TRAF-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052088">
        <property type="entry name" value="E3_ubiquitin-ligase_SINA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008974">
        <property type="entry name" value="TRAF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013010">
        <property type="entry name" value="Znf_SIAH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10315">
        <property type="entry name" value="E3 UBIQUITIN PROTEIN LIGASE SIAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10315:SF80">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SINAT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03145">
        <property type="entry name" value="Sina_TRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21361">
        <property type="entry name" value="Sina_ZnF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51081">
        <property type="entry name" value="ZF_SIAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SIAH-type" evidence="11">
        <location>
            <begin position="55"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9681160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="238" mass="26722" checksum="3B3C13080C813F98" modified="2024-01-24" version="1">MYLKVPTCSVISACASATMDTLCSSCKARVLNKCPSCRQQLGDIRCLALEKRAESLQLHCKYEEFGCPEIIPYYTKLMHEDSCNFRPYSCPCSGWECSVVGDIPLLVSHLTDYHKAVMFNSCDFKLGFLTEDLYKHPGCRWIVIIINCYGKHFCVHTEAFFTALTPIYVVFLSLIGNQAEVCNYSYSLQIGGNGRKLTFEGIPRSIRESERRSLESADSLIVLGSMVHSLGGGKKMKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DF27</accession>
    <name>A0AA39DF27_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DYW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_020407</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. PCMP-H subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9681519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DF27"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000344">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000900">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000348">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000305">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032867">
        <property type="entry name" value="DYW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046849">
        <property type="entry name" value="E2_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF445">
        <property type="entry name" value="DYW DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14432">
        <property type="entry name" value="DYW_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20430">
        <property type="entry name" value="Eplus_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="110"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="180"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="285"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="316"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="351"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="461"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="565"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="domain" description="DYW" evidence="4">
        <location>
            <begin position="780"/>
            <end position="872"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9681519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="872" mass="96966" checksum="1E7C868CCB21D251" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DGU4</accession>
    <name>A0AA39DGU4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription initiation factor TFIID subunit 8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_018911</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DGU4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08049">
        <property type="entry name" value="TAF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006565">
        <property type="entry name" value="BTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037818">
        <property type="entry name" value="TAF8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019473">
        <property type="entry name" value="TFIID_su8_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46338">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46338:SF19">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07524">
        <property type="entry name" value="Bromo_TP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10406">
        <property type="entry name" value="TAF8_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00576">
        <property type="entry name" value="BTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Bromodomain associated" evidence="8">
        <location>
            <begin position="23"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="8"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9683110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="377" mass="41319" checksum="AACAED796B488B85" modified="2024-01-24" version="1">MSDGGGESGREFDRTTKRKSSDRDFPQAIAKIAVAQICESAGFQGFQQSALETLSEVVVRYIRELGKTAHTYANSACRTECNIFDIIQGLEDLASLQGFSGASDSDHCLAGSGTVREIVQYVSEAEEIPLAHSVPHFPVSRDRKQTPSFLQIGEEPPGDHIPAWLPAFPDPQTYVHSPVLNERGADPCAGNIEQARQHKKAEWSLLNLQQQLACNGLEGPSMIDPGDAAKARRAAETNPFLSAPLHFGEKGVSPVFLPAKLSNEAVVENQAGENHAVANHVSVLETFAPAIELMKSRSCESEEGRKRVLSNQRPAVQFKIEIGKKSTGTALDLSFQNKDVEKITSWFGKDNEKDDKKRRAEKILKESMKNPQELAQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DGV0</accession>
    <name>A0AA39DGV0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pectinesterase inhibitor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_017873</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the PMEI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9681697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DGV0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046910">
        <property type="term" value="F:pectinesterase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15797">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.40">
        <property type="entry name" value="Invertase/pectin methylesterase inhibitor family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035513">
        <property type="entry name" value="Invertase/methylesterase_inhib"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052421">
        <property type="entry name" value="PCW_Enzyme_Inhibitor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006501">
        <property type="entry name" value="Pectinesterase_inhib_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034086">
        <property type="entry name" value="PMEI_plant"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01614">
        <property type="entry name" value="PME_inhib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36710:SF1">
        <property type="entry name" value="F14J9.2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36710">
        <property type="entry name" value="PECTINESTERASE INHIBITOR-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04043">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00856">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101148">
        <property type="entry name" value="Plant invertase/pectin methylesterase inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041466888" description="Pectinesterase inhibitor domain-containing protein" evidence="4">
        <location>
            <begin position="17"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="Pectinesterase inhibitor" evidence="5">
        <location>
            <begin position="20"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9681697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="171" mass="19208" checksum="F6242B43451EAA04" modified="2024-01-24" version="1">MVALLLSSSFFLPGSSVHPETERLIQDICQRVDNFQFCNTTMNANLRAYDEGIEDLAHLAILVALDSATRTKGYAEELTRQATDKDLKFCLKICSNSYAAVNDSFTAAKKQFDGRDFDAVDESEKKAAQPIKDCDFMFLRTSTRENPLLERNRQMSDLTQIALSALQNLLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DHF7</accession>
    <name>A0AA39DHF7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tubulin/FtsZ GTPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_017156</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the tubulin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DHF7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0493">Microtubule</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="5">
        <location>
            <begin position="28"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9685013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="93" mass="10495" checksum="1FF4F4B5D91BB4FA" modified="2024-01-24" version="1">MEIDLECEEDDGHWCQVGSIDSAVEGIERLFVDYGKKSKLGLTIYPSSQVSTFAIEPYNNVLSTHSLREHIDVVMLLDNEAIYDICRASLDIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DHS5</accession>
    <name>A0AA39DHS5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_016897</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DHS5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009570">
        <property type="term" value="C:chloroplast stroma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02947">
        <property type="entry name" value="TRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000245">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044253">
        <property type="entry name" value="WCRKC1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47192">
        <property type="entry name" value="THIOREDOXIN-LIKE 3-2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47192:SF4">
        <property type="entry name" value="THIOREDOXIN-LIKE 3-2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0249">Electron transport</keyword>
    <keyword evidence="3" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Thioredoxin" evidence="4">
        <location>
            <begin position="70"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9684656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="195" mass="22093" checksum="B48CFDDB503B3B8A" modified="2024-01-24" version="1">MSDTFRFYPSLTPLPPTTPRGSSRIPQQFSIPSLSISDFPRKSLNFPQKIGVSGGKVWALAAWNQEGPLQDLDDSPVSVELKPISSDTQFDRILGEAQQLEESVAIVWMATWCRKCIYLKPKLEKLAAEYYPRLRFYCVDVNTVPHKLVARAAVTKMPTIQLWKDSKKQAEVIGGHKAYFVVNEVREMIENECAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DI89</accession>
    <name>A0AA39DI89_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_016841</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DI89"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF78">
        <property type="entry name" value="OS12G0587133 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9684565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="216" mass="25379" checksum="14FD95CDB3CC6A18" modified="2024-01-24" version="1">MPLGAPFKSEMVWDGVEERFRKRLAMWKRQYISKGGRLTLIRSTLSSLPVYFMSLFHMPRKVRMRLEKIQRDFLWGGGALEQRPHLVRWNLVCLERKKGGLGVRNLALMNKALFGKWNWRFATERETLWKQVISQKYGVEDGGWCTRVASGRHGVGLWKAIRREWMGMYSNLAFRVGSGRRVRFWKDKWCGDEPLCESFPSLFSISLTKDVWVSDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DJX7</accession>
    <name>A0AA39DJX7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_015262</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 15">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PP2C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DJX7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000010">
        <property type="entry name" value="Probable protein phosphatase 2C 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="12">
        <location>
            <begin position="35"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9686275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="283" mass="31105" checksum="A3DDB647528CB769" modified="2024-01-24" version="1">MTGREILQKISVKVGLGSSTADTGKGKSKMLKHISHGFHLVKGKSNHEMEDCLVSEFKQVEDHELGLFGIFDGHLGHDVSNYLKTHLFDNILKEHTFWTETENAIKRAYRKTDIEILDKSLYLGRGGSTAVTAILINGERLVVANVGDSRAVICKNGEAKQLSVDHEPSKERTMIERRGGFVSNLPGDVPRVDGQLAVARAFGDKSLKLHLSSEPDVAVEPITNGTECIILASDGLWKVMSNQEAVDCIKHIKDAQSAAKRLTDEALSKKSKDDISCIVVKFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DNY5</accession>
    <name>A0AA39DNY5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_013809</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DNY5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF78">
        <property type="entry name" value="OS12G0587133 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="169"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9691733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="737" mass="84857" checksum="C160E888DB11E407" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DS95</accession>
    <name>A0AA39DS95_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Probable cytosolic iron-sulfur protein assembly protein CIAO1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_010552</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Essential component of the cytosolic iron-sulfur (Fe/S) protein assembly machinery. Required for the maturation of extramitochondrial Fe/S proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat CIA1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DS95"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000136">
        <property type="entry name" value="Probable cytosolic iron-sulfur protein assembly protein CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03037">
        <property type="entry name" value="ciao1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028608">
        <property type="entry name" value="CIAO1/Cia1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="16"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="63"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="107"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="152"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="202"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9695108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="349" mass="39026" checksum="56CFF44305723B76" modified="2024-01-24" version="1">MDFSEEGLELKEIQKLEGHNDRVWSLAWNPTSSLLASCSGDKTVRIWQRSPSTSSWHCKAVLEETHTRTVRSCAWSPSGNLLATASFDATTAIWELIGDDFECVSTLEGHENEVKSVSWNASGSLLATCSRDKSVWIWEVQPGNEFECVSVLQGHTQDVKMVQWHPIMDVLFSCSYDNTVKIWAEDGDSDDWHCVQTLGESNNGHTSTVWALSFNPEGDKMVTCSDDLTVKIWDTDSITMQAGEGYAPWKHLCTLSGYHDRTIFSVHWSREGIIATGAADDAIRFFVESKDGLVLTSILPLIHHIPHSHHQSAASLFPYCSCLFSIRYSVTCLFCLPTIPIHLVKLNLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DUY5</accession>
    <name>A0AA39DUY5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">U6 snRNA-associated Sm-like protein LSm4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LSM4</name>
        <name evidence="12" type="ORF">PVL29_008623</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Binds specifically to the 3'-terminal U-tract of U6 snRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">LSm subunits form a heteromer with a doughnut shape.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DUY5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120115">
        <property type="term" value="C:Lsm2-8 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01723">
        <property type="entry name" value="LSm4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000005">
        <property type="entry name" value="U6 snRNA-associated Sm-like protein LSm4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034101">
        <property type="entry name" value="Lsm4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027141">
        <property type="entry name" value="LSm4/Sm_D1/D3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23338">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 9" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="11">
        <location>
            <begin position="2"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="80"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="80"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="123"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9696490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="150" mass="16156" checksum="08C053FF1842E92F" modified="2024-01-24" version="1">MLPLSLLKTAQGHPMLVELKNGETYNGHLVNCDTWMNIHLREVICTSKDGDRFWRMPECYIRGNTIKYLRVPDEVIDKVQEETKNRADRKPPGVGRGRGRGRDESSSGRPAKGIGRGLDDGGARGMGGGRGRGGPGGKTGGSRGGGRGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DVD5</accession>
    <name>A0AA39DVD5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycosyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_009376</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="5"/>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DVD5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080043">
        <property type="term" value="F:quercetin 3-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080044">
        <property type="term" value="F:quercetin 7-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000057">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000756">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926">
        <property type="entry name" value="GLUCOSYL/GLUCURONOSYL TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926:SF1560">
        <property type="entry name" value="UDP-GLYCOSYLTRANSFERASE 74E1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9697524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="456" mass="50576" checksum="2C8B1917BE3947EE" modified="2024-01-24" version="1">MEKEKRTHKSHCIVLPYPSQGHINPMLQFSKRLVHNGAKVTLAVTCFISKSLLGDSGPITIKTISDGYDEGGFAQAESGGAYMERFRVVGSETLGSLIEKLKSSGCPVDCVVYDAFLPWALDVAKKLGLVGAVFFTQSCTVNNIYYHVHQGMLKLPLSEPEVVVPGLFPLQACDLPSLVYLYGSYPDFFNMLVNQFSNIEKVDWVFCNTFYKLEEKVVDWMAKICPLRTIGPTLPSAYLDKRLGDDKDYGLNMLKPVTGVCMEWLDSKPNGSVVYASYGSFAKLEPEQMEEVAWGLRRSNAYFLMVVRESEQAKLPQKFKEETAEKGLVVSWCPQLEVLAHRAIGCFLTHGGWNSTLEALSLGVPMVVAPLWVDQPTNAKFVEDVWGIGLRARANDKGIVRREVLEDCIGKVMGSDGLKEIKNNALKWKNLAREAVDEGGSSDKCIDEFVAKLTAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DW17</accession>
    <name>A0AA39DW17_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">General transcription factor IIH subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_007323</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the GTF2H2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DW17"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000439">
        <property type="term" value="C:transcription factor TFIIH core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005675">
        <property type="term" value="C:transcription factor TFIIH holo complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01453">
        <property type="entry name" value="vWA_transcription_factor_IIH_type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000449">
        <property type="entry name" value="General transcription factor IIH subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000015">
        <property type="entry name" value="General transcription factor IIH subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007198">
        <property type="entry name" value="Ssl1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004595">
        <property type="entry name" value="TFIIH_C1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012170">
        <property type="entry name" value="TFIIH_SSL1/p44"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00622">
        <property type="entry name" value="ssl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12695">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIH SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12695:SF2">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIH SUBUNIT 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07975">
        <property type="entry name" value="C1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04056">
        <property type="entry name" value="Ssl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015919">
        <property type="entry name" value="TFIIH_SSL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01047">
        <property type="entry name" value="C1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="VWFA" evidence="14">
        <location>
            <begin position="96"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="12">
        <location>
            <begin position="315"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="19"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR015919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR015919-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9698184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="433" mass="48359" checksum="A4C9F506F2D55423" modified="2024-01-24" version="1">MNDMEHRKTNNGEGRRLDGEEEEEEEDEDEGNGRGLDAWERAYADERSWESLQEDESGLLRPIDNKTIYHAQYRRRIRSLYSTTTTARIQKGLIRYLYIVVDLSRAAAEMDFKPSRMAVVAKHIEAFIREFFDQNPLSQIGLVTIKDGLAQCLTDLGGSPDSHVKALMGKLECSGDSSLQNALDLVHGYLNQIPSYGHREVLILYSALSTCDPGDIMETIQECKKSKIRCSVIGLSAEIFICRHLCQETGGSYSVALDESHFKELLLEHAPPPPAIAEFAIANLIKMGFPQRAAEGVISICSCHKEAKVGGGYTCPRCKARVCELPTECRICGLTLVSSPHLARSYHHLFPIPPFDEVSLSLLNNPHQRSSRACFGCQESLLIPGNKPNLCVACPKCKQHFCLDCDIYIHESLHNCPGCESFRHSKIVSVTEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DWG8</accession>
    <name>A0AA39DWG8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 17">Pectinesterase</fullName>
            <ecNumber evidence="6 17">3.1.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PVL29_008660</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Acts in the modification of cell walls via demethylesterification of cell wall pectin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 17">
            <text>[(1-&gt;4)-alpha-D-galacturonosyl methyl ester](n) + n H2O = [(1-&gt;4)-alpha-D-galacturonosyl](n) + n methanol + n H(+)</text>
            <dbReference type="Rhea" id="RHEA:22380"/>
            <dbReference type="Rhea" id="RHEA-COMP:14570"/>
            <dbReference type="Rhea" id="RHEA-COMP:14573"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17790"/>
            <dbReference type="ChEBI" id="CHEBI:140522"/>
            <dbReference type="ChEBI" id="CHEBI:140523"/>
            <dbReference type="EC" id="3.1.1.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 17">Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell envelope</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">In the C-terminal section; belongs to the pectinesterase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the PMEI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.11" evidence="6 17"/>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DWG8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004857">
        <property type="term" value="F:enzyme inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030599">
        <property type="term" value="F:pectinesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042545">
        <property type="term" value="P:cell wall modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045490">
        <property type="term" value="P:pectin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd15798">
        <property type="entry name" value="PMEI-like_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.40:FF:000004">
        <property type="entry name" value="Pectinesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000001">
        <property type="entry name" value="Pectinesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.40">
        <property type="entry name" value="Invertase/pectin methylesterase inhibitor family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035513">
        <property type="entry name" value="Invertase/methylesterase_inhib"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033131">
        <property type="entry name" value="Pectinesterase_Asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000070">
        <property type="entry name" value="Pectinesterase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006501">
        <property type="entry name" value="Pectinesterase_inhib_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31707">
        <property type="entry name" value="PECTINESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01095">
        <property type="entry name" value="Pectinesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04043">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00856">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101148">
        <property type="entry name" value="Plant invertase/pectin methylesterase inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00503">
        <property type="entry name" value="PECTINESTERASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 17" id="KW-0063">Aspartyl esterase</keyword>
    <keyword evidence="13" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="18" id="KW-0472">Membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="65"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="Pectinesterase inhibitor" evidence="19">
        <location>
            <begin position="83"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="active site" evidence="16">
        <location>
            <position position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00057335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAJ9696547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="613" mass="67057" checksum="67FD683541D510C9" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DXC6</accession>
    <name>A0AA39DXC6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_008206</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9699504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DXC6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000270">
        <property type="entry name" value="Alpha/beta-Hydrolases superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45763:SF28">
        <property type="entry name" value="ALPHA_BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45763">
        <property type="entry name" value="HYDROLASE, ALPHA/BETA FOLD FAMILY PROTEIN, EXPRESSED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="285"/>
            <end position="543"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9699504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="562" mass="63881" checksum="C8736E01A3A83E45" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DXU4</accession>
    <name>A0AA39DXU4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_005735</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DXU4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04051">
        <property type="entry name" value="C2_SRC2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044750">
        <property type="entry name" value="C2_SRC2/BAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32246:SF173">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32246">
        <property type="entry name" value="INGRESSION PROTEIN FIC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="250"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="2">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9700044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="290" mass="30615" checksum="34EA0E5409A0AF19" modified="2024-01-24" version="1">MEYRTLEINVISAKDLKDVNLISKMDVYVVVSIDGDSRSKQKTPVDRDGGTNPTWNFPMKFTVDESAAQQNRLTLSFKLRCDRTLGDKDIGEVNVPIKEFLDPAGESKPTQFVSYQVRKPSGKPKGELNLSYKFSAKSISQSATKAQEPVTAYPPAASVVPKADEPVTAYPAAVAASSSAYPPVGAYPPPPAGYGYPPQQPPPPGYGYPPPPAAAGYGYPPPQAGYGYPPQQGYGYQPAVQQPAKKKSKFGLGLGAGLLGGVVGGLLIGDLVSDAAAYDGGFDDGGFGDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DYS5</accession>
    <name>A0AA39DYS5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GDSL esterase/lipase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_004759</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DYS5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1110:FF:000003">
        <property type="entry name" value="GDSL esterase/lipase APG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051058">
        <property type="entry name" value="GDSL_Est/Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45648:SF167">
        <property type="entry name" value="GDSL ESTERASE_LIPASE LTL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45648">
        <property type="entry name" value="GDSL LIPASE/ACYLHYDROLASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_4G14700)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041301120" description="GDSL esterase/lipase" evidence="4">
        <location>
            <begin position="31"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9703121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="368" mass="40502" checksum="422E70DBF5186050" modified="2024-01-24" version="1">MDKVISMANSSVLVMVLIALLGTLAPLTEARAFFVFGDSLVDSGNNNYLVTSARADSPPYGIDYPTHRATGRFSNGLNIPDIISQKIKSESTLPYLSPQLTGKKLLVGANFASAGIGILNDTGVQFLNIIRIYTQLEYFQQYQQRLTALVGAQKARGIVNQALVLITLGGNDFVNNYYLVPNSARSRQFALPNYVRYLISEYQKILMRLYKLGARRVLVTGTGPMGCVPAERAMRSRNGECAAELQQASALFNPQLVQMLQGLNKKFHADVFIAANTHEMHMDFITDPQAFGFTTSKIACCGQGPYNGLGLCTVLSNLCSNRGQYAFWDAFHPSEKANRLIVQQIMTGSTMYMNPMNLSTIMALDSRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E1J3</accession>
    <name>A0AA39E1J3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_006901</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Golgi apparatus</location>
            <location evidence="8">trans-Golgi network membrane</location>
            <topology evidence="8">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the syntaxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E1J3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048193">
        <property type="term" value="P:Golgi vesicle transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21445">
        <property type="entry name" value="SNARE_NTD_AtSYP61-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15841">
        <property type="entry name" value="SNARE_Qc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.90:FF:000004">
        <property type="entry name" value="Syntaxin 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000034">
        <property type="entry name" value="syntaxin-61 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015260">
        <property type="entry name" value="Syntaxin-6/10/61_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12791">
        <property type="entry name" value="GOLGI SNARE BET1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05739">
        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09177">
        <property type="entry name" value="STX6_10_61_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="227"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="10">
        <location>
            <begin position="155"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9701722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="247" mass="28011" checksum="B711395D1B8CCF1F" modified="2024-01-24" version="1">MSSAQDPFYIVKEEIQESIDKLLSTFHQWERIPVETGEQVHLTKELLASCESIEWQVDELDKTISVAAKDPAWYGIDEVELDKRRRWTSTAHTQVSNVKKSVVAGKESNGTGTTNANGMRREMMRMSNPHQADRSNQYASQDNDDFISSESDRQLLLIRRQDEELDVLSASVERIGGVGLTIHEELLAQEKIIDELGSEMDSTSNRLDFVQKKVAMVMKKASAKGQIMMILFLVVLFIVLFVLVFLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E2G6</accession>
    <name>A0AA39E2G6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NmrA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_003017</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NmrA-type oxidoreductase family. Isoflavone reductase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E2G6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05259">
        <property type="entry name" value="PCBER_SDR_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000299">
        <property type="entry name" value="Bifunctional pinoresinol-lariciresinol reductase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008030">
        <property type="entry name" value="NmrA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050608">
        <property type="entry name" value="NmrA-type/Isoflavone_red_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045312">
        <property type="entry name" value="PCBER-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43349:SF35">
        <property type="entry name" value="PHENYLCOUMARAN BENZYLIC ETHER REDUCTASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43349">
        <property type="entry name" value="PINORESINOL REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05368">
        <property type="entry name" value="NmrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NmrA-like" evidence="4">
        <location>
            <begin position="3"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9704769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="308" mass="33973" checksum="5C19CC47F573B380" modified="2024-01-24" version="1">MSEKSKILIIGGTGYIGKFIVSASAKSGHSTFALVRESAVSNPSKSEIIESFKSSGVTLVYGNLYDHESLVKAIKQVDVVISTVGHAQLSDQVKIIAAIKEAGNVKRFFPSEFGNDVDRVHAVEPAKTAFEIKAQIRRTIEAEGIPYTYVSTNTFAGFFLPTFSQPGATAPPRDKVIILGDGNPKAVSNKEDDIGTYTIKAVDDPRTLNKVLYIRPPQNTYSFNDIVSLWEKKIGKTLEKIYVPEEQVLKNIQEASVPLNVILSICHSVFIKGDHTNFEIEQSFGVEATELYPDVKYTTVDEYLNQFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E338</accession>
    <name>A0AA39E338_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">MADS-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_003421</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E338"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00265">
        <property type="entry name" value="MADS_MEF2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1810.10:FF:000006">
        <property type="entry name" value="Agamous-like MADS-box protein AGL62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033896">
        <property type="entry name" value="MEF2-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11945:SF776">
        <property type="entry name" value="AGAMOUS-LIKE 50-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11945">
        <property type="entry name" value="MADS BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00404">
        <property type="entry name" value="MADSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="6">
        <location>
            <begin position="8"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9705369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="214" mass="24220" checksum="F3A41DFC782B5432" modified="2024-01-24" version="1">MATVRKSKGRQKVEMTKMTKESNLQVTFSKRRSGLFKKASELCTLCGAETAIIVFSPGKKVYSFGHPCVESILDRFLMRNPLPNSGSLQLFEAHRTTNVRKLNVQLTQVVNQLEGEKKRGEALTQMWKACKPQCWWAAPIEELSLEQLELLKVSLEDLRRKVARQADELMIEGKNPPAFFPGNSVGTIVPYAEWMAGFDPYSHKYNFGYGGPFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E4J4</accession>
    <name>A0AA39E4J4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Haloacid dehalogenase-like hydrolase domain-containing protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_000971</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E4J4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd16415">
        <property type="entry name" value="HAD_dREG-2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.720">
        <property type="entry name" value="Haloacid dehalogenase-like hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011949">
        <property type="entry name" value="HAD-SF_hydro_IA_REG-2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044924">
        <property type="entry name" value="HAD-SF_hydro_IA_REG-2-like_cap"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02252">
        <property type="entry name" value="DREG-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46649">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46649:SF5">
        <property type="entry name" value="F14L17.7 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00413">
        <property type="entry name" value="HADHALOGNASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9709271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="258" mass="28504" checksum="A37B9631474E5C22" modified="2024-01-24" version="1">MEACVARCSDGVFFRCLNRFNFRRSFMPMASIHAASDGGRCFNRAYDGLLLDAGGTLLQLAKPVESTYADIGRKYGLTASSAEIKQGFRRAFAAPWPEKLRYQGDGRPFWKLVVSEATGCANNDYFEEVYEYYANGDAWHLPTGASETMFLLKDAGVKLAVVSNFDTRLRKLLKDLNVLDLFDAVIISSEVGYEKPDAKIFKAALDQIGVEAGKAVHVGDDQEADKVGASAVGIDCWLWGTDVKTFSDIQKQILISEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E6Q6</accession>
    <name>A0AA39E6Q6_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_001876</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E6Q6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000010">
        <property type="entry name" value="Transcriptional activator Myb isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000016">
        <property type="entry name" value="Transcriptional activator Myb isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF252">
        <property type="entry name" value="TRANSCRIPTION FACTOR MYB3R-2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="48"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="48"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="100"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="100"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="152"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="156"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="20"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="20"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9706627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="593" mass="65343" checksum="94D70B801FBDEB99" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E7N5</accession>
    <name>A0AA39E7N5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GTP cyclohydrolase 1</fullName>
            <ecNumber evidence="5">3.5.4.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">GTP cyclohydrolase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_001010</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">GTP cyclohydrolase 1 is the first enzyme in the biosynthetic pathway leading to folic acid.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>GTP + H2O = 7,8-dihydroneopterin 3'-triphosphate + formate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17473"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58462"/>
            <dbReference type="EC" id="3.5.4.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; 7,8-dihydroneopterin triphosphate biosynthesis; 7,8-dihydroneopterin triphosphate from GTP: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GTP cyclohydrolase I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.16" evidence="5"/>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E7N5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003934">
        <property type="term" value="F:GTP cyclohydrolase I activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046656">
        <property type="term" value="P:folic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006729">
        <property type="term" value="P:tetrahydrobiopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1130.10:FF:000007">
        <property type="entry name" value="GTP cyclohydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1130.10:FF:000008">
        <property type="entry name" value="GTP cyclohydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.286.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1130.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043133">
        <property type="entry name" value="GTP-CH-I_C/QueF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043134">
        <property type="entry name" value="GTP-CH-I_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001474">
        <property type="entry name" value="GTP_CycHdrlase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020602">
        <property type="entry name" value="GTP_CycHdrlase_I_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11109:SF7">
        <property type="entry name" value="GTP CYCLOHYDROLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11109">
        <property type="entry name" value="GTP CYCLOHYDROLASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01227">
        <property type="entry name" value="GTP_cyclohydroI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55620">
        <property type="entry name" value="Tetrahydrobiopterin biosynthesis enzymes-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0783">Tetrahydrobiopterin biosynthesis</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="GTP cyclohydrolase I" evidence="15">
        <location>
            <begin position="20"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="GTP cyclohydrolase I" evidence="15">
        <location>
            <begin position="258"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00055676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9709329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="448" mass="49069" checksum="E1A1928BBE98896A" modified="2024-01-24" version="1">MGVLDEGHVEVGFEQEALAIEEAVKVLLQGLGEDINREGLSKTPARVAKALREGTRGYRQKAKDIVQGALFPEAGLNNGIGHAGGVGGLVIVRDLDLFSYCESCLLPFQVKCHVGYVPSGQCVVGLSKLSRVADVFAKRLQDPQRLADDVCSALNHGIKPAGVAVVLQCLHIHFPNLELGSLDSTHQEWVKVSVCSGKGLFENAKATIWSDFLSLLKFRGINVEKTCTRDSTGPCWCPSQSFSALIPCKIETVHQMVTAVTSILRSLGEDPLRKGLVGTPNRYVRWLLNFEKTNMELKLDGYVCGKMDPFQPNCNEEEMHSELNLAFWSQCEHHLLPFHGVVHIGYFCTKGTNPIAKSILQSIVHFYGFKLQVQERITRQVAETVSSLLGGDVIVVVEASHSCMVSRGIEKLGSNTATMALLGRFSTDDAAKTMFLQRIQNTSTSRGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E8U3</accession>
    <name>A0AA39E8U3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">PVL29_001126</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="23">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E8U3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048638">
        <property type="term" value="P:regulation of developmental growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000021">
        <property type="entry name" value="Putative LRR receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000015">
        <property type="entry name" value="Somatic embryogenesis receptor kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000016">
        <property type="entry name" value="Somatic embryogenesis receptor-like kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47988">
        <property type="entry name" value="SOMATIC EMBRYOGENESIS RECEPTOR KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="14 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8 21" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041464527" description="non-specific serine/threonine protein kinase" evidence="21">
        <location>
            <begin position="25"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="241"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="305"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="333"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAJ9709495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="625" mass="69341" checksum="27D48DFBFB525E38" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YGA2</accession>
    <name>A0AA38YGA2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Acyl-[acyl-carrier-protein] desaturase</fullName>
            <ecNumber evidence="16">1.14.19.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_026456</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Introduction of a cis double bond between carbons of the acyl chain.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="15">Binds 2 iron ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="16">Binds 2 Fe(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; fatty acid metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 16">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the fatty acid desaturase type 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.19.-" evidence="16"/>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9669897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YGA2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009570">
        <property type="term" value="C:chloroplast stroma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045300">
        <property type="term" value="F:stearoyl-[ACP] desaturase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004768">
        <property type="term" value="F:stearoyl-CoA 9-desaturase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01050">
        <property type="entry name" value="Acyl_ACP_Desat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.620.20:FF:000002">
        <property type="entry name" value="Stearoyl-[acyl-carrier-protein] 9-desaturase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.620.20">
        <property type="entry name" value="Ribonucleotide Reductase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005803">
        <property type="entry name" value="FADS-2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005067">
        <property type="entry name" value="Fatty_acid_desaturase-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012348">
        <property type="entry name" value="RNR-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31155">
        <property type="entry name" value="ACYL- ACYL-CARRIER-PROTEIN DESATURASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31155:SF9">
        <property type="entry name" value="STEAROYL-[ACYL-CARRIER-PROTEIN] 9-DESATURASE 7, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03405">
        <property type="entry name" value="FA_desaturase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000346">
        <property type="entry name" value="Dlt9_acylACP_des"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00574">
        <property type="entry name" value="FATTY_ACID_DESATUR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 16" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="14 16" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="12 15" id="KW-0408">Iron</keyword>
    <keyword evidence="5 16" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0934">Plastid</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <feature type="binding site" evidence="15">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000346-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9669897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="393" mass="45020" checksum="D7EC5DAF9CCDFAAC" modified="2024-01-24" version="1">MALKLNSAFVCPYHNPRPQSRRPRSHRVFMASTLRPTSIEVENPKKPFSPPREVHVQVTHSLPPQKIEVFKSMEGWAEKNILVHLKPVEKCWQPQDFLPDPTSEGFYEQVEELRERAKELPDDYFVVLVGDMITEEALPTYQTMLNTLDGVRDETGASLTPWAVWTRAWTAEENRHGDLLNKYLYLSGRVDMKQIEKTIQYLIGSGMDPRTENSPYLGFIYTSFQERATFISHGNTARLAKEHGDMKLAQICGTIAADEKRHETAYTKIVEKLFEIDPDGTVLAFADMMRKKISMPAHLMYDGQDDNLFEHFSAVAQRLGVYTAKDYADILEFLVGRWNLEKLTGLSGEGRKAQDYVCGLAPRIRKLEERAQGRVKQATAVPFSWIFGRKMMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YGY4</accession>
    <name>A0AA38YGY4_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_026658</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YGY4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040262">
        <property type="entry name" value="At4g38062-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45287">
        <property type="entry name" value="OS03G0691500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45287:SF4">
        <property type="entry name" value="OS03G0691500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1107"/>
            <end position="1134"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="172"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="239"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="313"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="418"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="561"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="871"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="973"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1123"/>
            <end position="1134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9670247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1156" mass="135478" checksum="0ABE4427E65FA0B1" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YHI0</accession>
    <name>A0AA38YHI0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">procollagen-proline 4-dioxygenase</fullName>
            <ecNumber evidence="4">1.14.11.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_026826</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-prolyl-[collagen] + 2-oxoglutarate + O2 = trans-4-hydroxy-L-prolyl-[collagen] + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:18945"/>
            <dbReference type="Rhea" id="RHEA-COMP:11676"/>
            <dbReference type="Rhea" id="RHEA-COMP:11680"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:50342"/>
            <dbReference type="ChEBI" id="CHEBI:61965"/>
            <dbReference type="EC" id="1.14.11.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the P4HA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.2" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YHI0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004656">
        <property type="term" value="F:procollagen-proline 4-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.620:FF:000002">
        <property type="entry name" value="Prolyl 4-hydroxylase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045054">
        <property type="entry name" value="P4HA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044862">
        <property type="entry name" value="Pro_4_hyd_alph_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869:SF246">
        <property type="entry name" value="TRANSMEMBRANE PROLYL 4-HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13640">
        <property type="entry name" value="2OG-FeII_Oxy_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00702">
        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0408">Iron</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="16">
        <location>
            <begin position="160"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9670532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="285" mass="32208" checksum="9A07C2A81769053D" modified="2024-01-24" version="1">MKSKAKGKWRFGSKLGLPVVFFFCLFFFLAGLFGSGLLPQEFSSSEPRRLIREERDYDPLAHGESGEDSVTSIPFQVLSWRPCALYFPNFATSEQCQSIINMAKSNLTPSTVALRVGEIRENTEGIRTSSGVFISASEDKTGILDLIEQKIARVIMIPRTHGEAFNVLRYEIGQRYNSHYDAFDPAEYGPQKSHRIATFLVYLSDVEEGGETMFPFENGLNMNKDYDFQRCIGLKVKPHQGDGLLFYSMFPNGTIDPTSLHGSCPVIKGEKWVATKWIRDQEQDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YI08</accession>
    <name>A0AA38YI08_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_027020</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YI08"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47943:SF2">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47943">
        <property type="entry name" value="CYTOCHROME P450 93A3-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10 11" id="KW-0349">Heme</keyword>
    <keyword evidence="7 10 11" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 11" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041247231" evidence="12">
        <location>
            <begin position="18"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="10">
        <location>
            <position position="378"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9670831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="439" mass="49749" checksum="3161682AF405AF59" modified="2024-01-24" version="1">MTVWTWTALALTAIIFSNIMKNKHKRLPPGPKAIPILGNMHMLGNLPHRALQALAKKYGPIMYMRLGFVPAIIVSSPQAVEQFLKTHDLAFSNKPPYDMRKLCTLELLTNHKINSFKPMRREEVGLLIKSFKEAGRAGVVVDVNAKVALLTTDMSCRMVFGKKYMDKDLDERGFKVVFQEAMQLAATSNIGDYIPCLLGLDFQGLTRWIKATSKVFDDFFEKIIDEHIHNPKEKGQTKDLVDVMLGLMGSEGTEYNIERASIKAISFDMMAGSMDTSSSSIDWAVAELIRHPRVMKKVQSELEKVVGMERMVEESDLESLEYLNMVYRIMVNAWTIGQDPDSWTNADEFLLERFIEGDIDFRGKHFQYIPFGSGRRGCPGMQLGLTVVRFVVAQLVHCFDWELPDGMMLPSELDMTEELGLAIPRAKHLVAIPTYRICQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YIG6</accession>
    <name>A0AA38YIG6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_027174</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9671053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YIG6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005162">
        <property type="entry name" value="Retrotrans_gag_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03732">
        <property type="entry name" value="Retrotrans_gag"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Retrotransposon gag" evidence="2">
        <location>
            <begin position="91"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9671053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="190" mass="22265" checksum="B7DC33B7E1826DF8" modified="2024-01-24" version="1">MPRARNRLTTRLRQTTRSSPLSHGNVTEGRATIYLVAGSEYQNVSLIRELKALTPPIFRGGPNFLEAQNWMKEIKKIPDAIAVPEEMRVSLAFFMLRDEADNWWDMIKTTQDVTQMVWMQFEELLLSNYFPEAVRRQKRVEFIHLVQRNMIVLEYATKFTQLSKFASNVVPDELMRAEQFQEGLRLNIRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YJ11</accession>
    <name>A0AA38YJ11_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">alpha,alpha-trehalose-phosphate synthase (UDP-forming)</fullName>
            <ecNumber evidence="3">2.4.1.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_025161</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the trehalose phosphatase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the N-terminal section; belongs to the glycosyltransferase 20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.15" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YJ11"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003825">
        <property type="term" value="F:alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004805">
        <property type="term" value="F:trehalose-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03788">
        <property type="entry name" value="GT20_TPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01627">
        <property type="entry name" value="HAD_TPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000100">
        <property type="entry name" value="Alpha,alpha-trehalose-phosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000046">
        <property type="entry name" value="alpha,alpha-trehalose-phosphate synthase [UDP-forming] 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000039">
        <property type="entry name" value="alpha,alpha-trehalose-phosphate synthase [UDP-forming] 1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001830">
        <property type="entry name" value="Glyco_trans_20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012766">
        <property type="entry name" value="Trehalose_OtsA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003337">
        <property type="entry name" value="Trehalose_PPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011071">
        <property type="entry name" value="PRK14501.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02400">
        <property type="entry name" value="trehalose_OtsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788:SF130">
        <property type="entry name" value="ALPHA,ALPHA-TREHALOSE-PHOSPHATE SYNTHASE [UDP-FORMING] 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788">
        <property type="entry name" value="TREHALOSE-6-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00982">
        <property type="entry name" value="Glyco_transf_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02358">
        <property type="entry name" value="Trehalose_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="831"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="16"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="32"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="834"/>
            <end position="845"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9671330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="938" mass="105294" checksum="6F3BC67939B708AE" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YJQ3</accession>
    <name>A0AA38YJQ3_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_025403</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YJQ3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9671676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="68" mass="7596" checksum="8DAD5BE4E4E2CE07" modified="2024-01-24" version="1">MNVVKRVITWISISLSLWLILVGARRVNSSYKEPVVMERGPVPPSASSSCTYIGGDNRDQYRRCPPST</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YKM0</accession>
    <name>A0AA38YKM0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">TIR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_025709</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YKM0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TIR" evidence="1">
        <location>
            <begin position="20"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9672183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="48" mass="5412" checksum="F89445102EAE149D" modified="2024-01-24" version="1">MASPSIQRPSSSSSTSHSRWSYDVFLSFRGEDTRISFTDHLYSALISL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YLB6</accession>
    <name>A0AA38YLB6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">THO complex subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_025918</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the THOC3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YLB6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000445">
        <property type="term" value="C:THO complex part of transcription export complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000300">
        <property type="entry name" value="THO complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000390">
        <property type="entry name" value="THO complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040132">
        <property type="entry name" value="Tex1/THOC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22839:SF0">
        <property type="entry name" value="THO COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22839">
        <property type="entry name" value="THO COMPLEX SUBUNIT 3 THO3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25174">
        <property type="entry name" value="Beta-prop_THOC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="15"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="61"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="186"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00046343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9672494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="313" mass="35166" checksum="228E83650B47AEBE" modified="2024-01-24" version="1">MEETIPFKNLHSREYQGHKKKVHSVAWNCTGTKLASGSVDQTARIWLIEQHGHGKVKDIELKGHTDSVDQLCWDPKHADLIATASGDKTVRLWDARSGKCTQQAELSGENINITYKPDGTHIAVGNRDDELTILDVRKFKPIHRRKFSYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPALRPLDTLMAHTAGCYCIAIDPIGRYFAVGSADSLVSLWDISEMLCVRTFTKLEWPVRTISFNHTGEYIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQADEGVFRIFGFESA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YNX3</accession>
    <name>A0AA38YNX3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_023478</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YNX3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000760">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At5g27460"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:002288">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At5g27460"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001916">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45717:SF13">
        <property type="entry name" value="OS02G0796400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45717">
        <property type="entry name" value="OS12G0527900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13812">
        <property type="entry name" value="PPR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="349"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9673951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="502" mass="57663" checksum="2C0AF46229C75053" modified="2024-01-24" version="1">MAFRCLFFTLRRFAYSSRRSISSKTTPMDEAACTFKRDDLTSRMLKLVFPRRSATAVLQNWIDQGHKVTASDLRNVSRQLLKSKRHKHALEILAWMEAQNRFQMSAADHAIRLELIIKIQSLAEAEEYFKHLPNSSSRKAACLPLLHAYVKERAIEKAEALMLKLNDLGLTVSPHPFNEMMKLYMATSQFERVPTVILQMKQNKIPLNVLSYNLWMSACSEVSGVASAEMVYKEMVDDKNMEVGWSTLSTLANIYLKSGLIKKANLALKNAEKKLSAHNRLGYFFLITMYASLSNKEEVLRLWETSKKVGGRITSTNYMCILLCLVKLGDIAEAERIFREWESKCWKYDIRVSNVLLGAYMRTGSMDKAESLHLHTLERGGCPNYKTWEILMEGWMKSQNMDKAINAMKKGFSMLKHCEWRPNPGLVMAIAGYLEEHGNVNDLNQYVKVIHQLGIANLPLYKSLLRMHLLFQRPVFDILKMMEKDEIEMDDETSALVQACKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YPX6</accession>
    <name>A0AA38YPX6_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_023818</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YPX6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9674493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="110" mass="11576" checksum="47FB6834B0A06E6F" modified="2024-01-24" version="1">MAKVKLIMSIYVFILALVLIYGGLLSEGRKLDVEKNSKCEMGVSIDEKIGVLGNLHRSSKANAQPNAPVRQSPGADRLITDNGVDVQSTTPGHSPGVGHSVGPSSNEPNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YQ39</accession>
    <name>A0AA38YQ39_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Coenzyme Q-binding protein COQ10 START domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_023764</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Required for the function of coenzyme Q in the respiratory chain. May serve as a chaperone or may be involved in the transport of Q6 from its site of synthesis to the catalytic sites of the respiratory complexes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Interacts with coenzyme Q.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the COQ10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YQ39"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048039">
        <property type="term" value="F:ubiquinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045333">
        <property type="term" value="P:cellular respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07813">
        <property type="entry name" value="COQ10p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044996">
        <property type="entry name" value="COQ10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005031">
        <property type="entry name" value="COQ10_START"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12901:SF10">
        <property type="entry name" value="COENZYME Q-BINDING PROTEIN COQ10, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12901">
        <property type="entry name" value="SPERM PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03364">
        <property type="entry name" value="Polyketide_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Coenzyme Q-binding protein COQ10 START" evidence="4">
        <location>
            <begin position="96"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9674414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="246" mass="27717" checksum="9814EFF788F244FD" modified="2024-01-24" version="1">MPPFFTTARAVGSFVCRSNCVRSAKNGGQSRMLFNQTRCLSGIAPVHESLLANKDFNTLTGRTCCSSAVQRRSFLGCVDGEEGGILSKVTEERRIIGYSPEQLFDVVAAVDLYHGFVPWCQQSQIIQRYPDGSFDAELEIGFKFLVENYVSHVELNRPKCIKTTVSESSLFDHLINTWEFNPGPSPGTCNLYFLVDFKFQSPLYRQVASMFFKEVVSRLVGSFSERCRLIYGPGVPILENSYERRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YRP4</accession>
    <name>A0AA38YRP4_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_024287</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YRP4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9675269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="48" mass="5733" checksum="BF0D9D6B62DC4CAC" modified="2024-01-24" version="1">MHCRDLFSGHQHQYPSSVNRHFGRRVTKLAIEHHWNLRIPMNPRTRGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YT38</accession>
    <name>A0AA38YT38_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_024847</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9676052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YT38"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9676052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="56" mass="6399" checksum="DBEE49BBEA7708DE" modified="2024-01-24" version="1">MKANCMNSTVLAEALFAQEMKKSQVEMFKSSKQDQQDRYLIKGLSNKIPPDDIAYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YVD6</accession>
    <name>A0AA38YVD6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ketoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_022356</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YVD6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005559">
        <property type="entry name" value="PRK07231.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42820">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42820:SF21">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE REDUCTASE 3B-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00822">
        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ketoreductase" evidence="2">
        <location>
            <begin position="14"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9677329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="262" mass="27667" checksum="F9571E24B753FF94" modified="2024-01-24" version="1">MADRSPSQNNLEGKVAIITGGASGIGEATARHFANHGAGAIVIADIQAEKGQLVAESIGLHRCRYIPCDVTDEQQVKAVVESTVQVYGQLDVMFCNAGIMSVGMQDVLDFDLSAYDTLFAINVRGVAASVKHAARAMVEGKVKGSIICTASVSASTGSDKFIDYVMSKMAVLGLVKSASRQLGAYGIRVNSVSPGAVATPLLCDKFQMSVTEVENNFEQYMSLKGVGPMKEKDVADAVLFLASDNSKFVTGHNLIVDGGYPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YW50</accession>
    <name>A0AA38YW50_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Replication protein A subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PVL29_022540</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12 14">Component of the replication protein A complex (RPA) required for DNA recombination, repair and replication. The activity of RPA is mediated by single-stranded DNA binding and protein interactions. Probably involved in repair of double-strand DNA breaks (DSBs) induced by genotoxic stresses.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13 14">Heterotrimer of RPA1, RPA2 and RPA3 (canonical replication protein A complex).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the replication factor A protein 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YW50"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04474">
        <property type="entry name" value="RPA1_DBD_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04475">
        <property type="entry name" value="RPA1_DBD_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04476">
        <property type="entry name" value="RPA1_DBD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000041">
        <property type="entry name" value="Replication protein A subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000064">
        <property type="entry name" value="Replication protein A subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000090">
        <property type="entry name" value="Replication protein A subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000257">
        <property type="entry name" value="Replication protein A subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047192">
        <property type="entry name" value="Euk_RPA1_DBD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013955">
        <property type="entry name" value="Rep_factor-A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007199">
        <property type="entry name" value="Rep_factor-A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031657">
        <property type="entry name" value="REPA_OB_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004591">
        <property type="entry name" value="Rfa1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003871">
        <property type="entry name" value="RFA1B/D_OB_1st"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00617">
        <property type="entry name" value="rpa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47165">
        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47165:SF4">
        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02721">
        <property type="entry name" value="DUF223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04057">
        <property type="entry name" value="Rep-A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08646">
        <property type="entry name" value="Rep_fac-A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16900">
        <property type="entry name" value="REPA_OB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 14" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Replication factor-A protein 1 N-terminal" evidence="17">
        <location>
            <begin position="5"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="Replication protein A 70 kDa DNA-binding subunit B/D first OB fold" evidence="16">
        <location>
            <begin position="179"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Replication protein A OB" evidence="19">
        <location>
            <begin position="309"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="Replication factor A C-terminal" evidence="18">
        <location>
            <begin position="468"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="12"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00058063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00065674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU364130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF08646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF16900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAJ9677607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="625" mass="69860" checksum="16BBE19D20D3E711" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YWS1</accession>
    <name>A0AA38YWS1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_022887</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9678126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YWS1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003906">
        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008311">
        <property type="term" value="F:double-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004808">
        <property type="entry name" value="AP_endonuc_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748">
        <property type="entry name" value="AP ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748:SF11">
        <property type="entry name" value="OS07G0184032 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9678126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="171" mass="19748" checksum="CC88CDD3A41EBEC0" modified="2024-01-24" version="1">MKIISWNTRGLGSRKKRRVVKDFLRLENPDVVMFQETKREICDRRFVGSVWSVRNKEWATFPACGASGEILIMIIWDSKKLSSEEVVIGSFFVSIKFLLDGCKPLWLFAVYGPNSPLLRKDFWVDLLDIFGLSFPLWCVDGDFNVIRRSSEKLGGSSFTSSMRDFDGYKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YX14</accession>
    <name>A0AA38YX14_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Glutamate decarboxylase</fullName>
            <ecNumber evidence="3 10">4.1.1.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_022835</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-glutamate + H(+) = 4-aminobutanoate + CO2</text>
            <dbReference type="Rhea" id="RHEA:17785"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:59888"/>
            <dbReference type="EC" id="4.1.1.15"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8 9">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the group II decarboxylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.15" evidence="3 10"/>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9678072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YX14"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004351">
        <property type="term" value="F:glutamate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006538">
        <property type="term" value="P:L-glutamate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06450">
        <property type="entry name" value="DOPA_deC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000022">
        <property type="entry name" value="Glutamate decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.160:FF:000001">
        <property type="entry name" value="Glutamate decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.50:FF:000002">
        <property type="entry name" value="Glutamate decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010107">
        <property type="entry name" value="Glutamate_decarboxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01788">
        <property type="entry name" value="Glu-decarb-GAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43321">
        <property type="entry name" value="GLUTAMATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43321:SF22">
        <property type="entry name" value="GLUTAMATE DECARBOXYLASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="4 10" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="7 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="6 8 9" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="8">
        <location>
            <position position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR602129-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9678072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="501" mass="56568" checksum="A7A72AC68AA42206" modified="2024-01-24" version="1">MAISTTISDSSEHLHSTFASRYVRDPLPKFKMPERSIPKEAAFQIISDELMLDGNPRLNLASFVTTWMEEECDKLIMASVNKNYVDMDEYPVTTELQNRCVNMIAHLFNAPVGENETAIGVGTVGSSEAIMLAGLAFKRKWQQKRKSEGKPYDKPNIVTGANVQVCWEKFARYFEVELKEVKLREGYYVMDPVKAVEMVDENTICVAAILGSTLTGEFEDVKLLNELLTEKNKETGWGTPIHVDAASGGFIAPFLYPDLKWDFRLPLVKSINVSGHKYGLVYAGVGWVVWRTKDDLPEELIFHINYLGSDQPTFTLNFSKGSSQIIAQYYQFIRLGFEGYKNVMRNCVDNANVLRQGLVETGRFEIVSKDIGVPLVAFSLKDTSNYTVFEISANLRRFGWIIPAYTMPPDAQHIAVLRVVVREDFSRSLAERLVSDIEKVMREMDAVQSRAATRATHVIATVDETREGKGEGSEKLNKKTGEEIAMYWKRVVDRKKTNGVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YXD3</accession>
    <name>A0AA38YXD3_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_020577</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YXD3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9678435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="54" mass="6008" checksum="79CF097045776F82" modified="2024-01-24" version="1">MKELCGMWLIPGATLWVYFSQSSSSSNGCTSGSSHHYRFALEDELLYLPSCNTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z0I9</accession>
    <name>A0AA38Z0I9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_021785</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9680015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z0I9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10827:SF85">
        <property type="entry name" value="CALCIUM-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10827">
        <property type="entry name" value="RETICULOCALBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13202">
        <property type="entry name" value="EF-hand_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041378620" description="EF-hand domain-containing protein" evidence="2">
        <location>
            <begin position="26"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="168"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9680015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="195" mass="22761" checksum="1482203538AD4696" modified="2024-01-24" version="1">MSKAVVSSLLTIAFVLFVVFTPTDQKGHNNFYLARRLGYNQVAFDPLAEEIEEMAREASDHHFLFNLPNIFLNDVQDKYEFVSQVKNLNITMRLMVLFPLLDVEPRDGFISLKELEHWNMQQAIHRLNYRTHKELVLFDQNEDGAITFSEYLPKISYQSIENNGMTHGEAGWWEDQFKNADFDNNGALGFEEFKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z1M2</accession>
    <name>A0AA38Z1M2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glycoprotease 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">GCP1</name>
        <name evidence="11" type="ORF">PVL29_019897</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in mitochondrial tRNAs that read codons beginning with adenine. Probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. Involved in mitochondrial genome maintenance.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>L-threonylcarbamoyladenylate + adenosine(37) in tRNA = N(6)-L-threonylcarbamoyladenosine(37) in tRNA + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:37059"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10163"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74418"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.3.1.234"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="9">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the KAE1 / TsaD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z1M2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061711">
        <property type="term" value="F:tRNA N(6)-L-threonylcarbamoyladenine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002949">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd24134">
        <property type="entry name" value="ASKHA_NBD_OSGEPL1_QRI7_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000083">
        <property type="entry name" value="Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000133">
        <property type="entry name" value="Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01445">
        <property type="entry name" value="TsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000905">
        <property type="entry name" value="Gcp-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017861">
        <property type="entry name" value="KAE1/TsaD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022450">
        <property type="entry name" value="TsaD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00329">
        <property type="entry name" value="gcp_kae1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03723">
        <property type="entry name" value="T6A_TsaD_YgjD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735">
        <property type="entry name" value="TRNA N6-ADENOSINE THREONYLCARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735:SF6">
        <property type="entry name" value="TRNA N6-ADENOSINE THREONYLCARBAMOYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00814">
        <property type="entry name" value="TsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00789">
        <property type="entry name" value="OSIALOPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="3 9" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Gcp-like" evidence="10">
        <location>
            <begin position="98"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9680718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="468" mass="50934" checksum="B69096238D271EA9" modified="2024-01-24" version="1">MVSLATSTFSRNLCPRPSLPYAVRALGTTLLRLHPKPTSWGPLSSSLKSCSAHFSASNFKFSKSPNETLGSRDDLVVLGIETSCDDTAAAIVRSNGDILSQVVSSQADLLARYGGVAPKMAEGAHMQVIDQVVQDALENANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSHNLPIVGVHHMEAHALVARLIEKDLQFPFMALLISGGHNLLILARDLGHYIQLGTTIDDAIGEAYDKTAKWLGLDMRRSGGPAIEELAREGDAKAVKFSTPMKQHKDCNFSYAGLKTQVRLAIESRNINAEIPISSASSEDRSSRADIAASFQRVAVLHLEERCERAIEWALKIEPSIKHLVVSGGVASNQYVRAQLDQVVKKKSLQLVCPPPSLCTDNGVMVAWTGLEHFCMGRYDPPPPANEPEDYVYDLRPRWPLGEEYSEGRSEARSLRTARIHPSLTSLIQASMQQQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z1V0</accession>
    <name>A0AA38Z1V0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_019852</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z1V0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF78">
        <property type="entry name" value="OS12G0587133 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9680657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="577" mass="66122" checksum="E8751E45340732DB" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z253</accession>
    <name>A0AA38Z253_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_020008</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z253"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11073">
        <property type="entry name" value="CYP76-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000007">
        <property type="entry name" value="Cytochrome P450 76C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47950">
        <property type="entry name" value="CYTOCHROME P450, FAMILY 76, SUBFAMILY C, POLYPEPTIDE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47950:SF44">
        <property type="entry name" value="CYTOCHROME P450, FAMILY 76, SUBFAMILY C, POLYPEPTIDE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0349">Heme</keyword>
    <keyword evidence="4 5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="5">
        <location>
            <position position="439"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9680870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="496" mass="55515" checksum="7AB427693CEBB0E6" modified="2024-01-24" version="1">MDYTTLLLLLSFVWSCIIVITIGFTNRKSGVARLPPGPRPFPIIGNLLQLGEKPHQSLAILSKTYGPLMSLKLGTTTTIVVSSSEAAQEVLNKNDQAFSSRTVLNAIQVADHHHFSIVFLPASAHWRNLRKICSMQMFSSHRVDAGQAMRQNIVQQLLGHAQESCSSGRAVDIGRATFTTTLNLLSNTIFSVNLAHYNSNFSQEFKDLIWSIMEEAGKPNLADFFPVLRLVDPQGIQKRMTVCFNKLFEVFDGFIEQRLPLKASSANNDVLDGLLNLDKQHDHELSSNDVRHLFVDLFSAGTDTTSSTVEWAMAELLNNPKSMARARSELGKVVGKEKIVEESDISKLPYLQAVVKETFRLHPPVPFLVPRKTEMKSEILGYAVPKNAHVLVNVWAIGRDSTIWSNPNSFVPERYLECEIDVKGRDFQLIPFGAGRRICPGLLLGHRMVHLMLASLVHSFDWKLEDGLKPEDMDMTEKFGFTLRKAQPLQAVPIKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z3J1</accession>
    <name>A0AA38Z3J1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ribosomal RNA small subunit methyltransferase G</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_020347</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9681432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z3J1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000323">
        <property type="term" value="C:lytic vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035493">
        <property type="term" value="P:SNARE complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15157:SF25">
        <property type="entry name" value="OS07G0418000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15157">
        <property type="entry name" value="UV RADIATION RESISTANCE-ASSOCIATED GENE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9681432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="268" mass="30438" checksum="7891AAA76320C403" modified="2024-01-24" version="1">MGDLYALDFDGILCDSCGESSLSAVKAAKVRWPGLFDGVDSKLEDWIVDQMFIIRPVVETGYENLLLVRLLLEMRLPSIRKSSVAEGLTIEGILENWSKLKPVIMEEWDEKREPLVDLFGKVRDEWMEDDLATWIDANRFYPGVADALKFASSKVYIVTTKQGRFAEALLRELAGVTIPSERIYGLGTGPKVEVLKQLQKQSEHQGLTLHFVEDRLATLKNVIKEPELDGWNLYLGKWGYNTQKEREEAAGISRVQLLELSDFSKKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z4I0</accession>
    <name>A0AA38Z4I0_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_018121</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z4I0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="136"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9682082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="186" mass="20347" checksum="26D343604B282308" modified="2024-01-24" version="1">MDQRHNHLESLGICYRLFNFISKTLESQALKLMILCRPLSHGLAICHRLFNFTMQTLAAQAVKPVILGQPGHQPLGQVPISDASAPIATITSPRSVLNKGYKELPIGPGTGLKKSVSINDTVEEIPNTSKSRKKKMAPEKLASLEREKGEEPKPLKSILKVGSNLTGKSVSFTIPPIDAKMDEAQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z4U5</accession>
    <name>A0AA38Z4U5_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_018263</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z4U5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9682295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="75" mass="8435" checksum="EBF6001CB244348F" modified="2024-01-24" version="1">MALFGFDSISFRDFDGTIRNMIPYVVEGIMFFVCYSISIIFFNIGSSLATTRDRRGLSHRGGNILFNTELGSARN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z6W0</accession>
    <name>A0AA38Z6W0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Peptidase metallopeptidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_019192</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="11">Can bind about 5 Ca(2+) ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M10A family. Matrix metalloproteinases (MMPs) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z6W0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030574">
        <property type="term" value="P:collagen catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04278">
        <property type="entry name" value="ZnMc_MMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000018">
        <property type="entry name" value="Metalloendoproteinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033739">
        <property type="entry name" value="M10A_MMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001818">
        <property type="entry name" value="Pept_M10_metallopeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021190">
        <property type="entry name" value="Pept_M10A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002477">
        <property type="entry name" value="Peptidoglycan-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036365">
        <property type="entry name" value="PGBD-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201">
        <property type="entry name" value="MATRIX METALLOPROTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201:SF304">
        <property type="entry name" value="PEPTIDASE METALLOPEPTIDASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00413">
        <property type="entry name" value="Peptidase_M10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01471">
        <property type="entry name" value="PG_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00138">
        <property type="entry name" value="MATRIXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47090">
        <property type="entry name" value="PGBD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0732">Signal</keyword>
    <keyword evidence="6 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041243942" description="Peptidase metallopeptidase domain-containing protein" evidence="13">
        <location>
            <begin position="26"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase metallopeptidase" evidence="14">
        <location>
            <begin position="148"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Cysteine switch" evidence="12">
        <location>
            <begin position="115"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="260"/>
        </location>
    </feature>
    <feature type="binding site" description="in inhibited form" evidence="11">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR621190-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR621190-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR621190-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9683511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="303" mass="32779" checksum="3C6F0977BD783322" modified="2024-01-24" version="1">METKVSSMLSVFVSSLLLLPFLSQATSPDLSPFGFFKDLQGSKKGDKVEGIHKVRKYLQRFGYMSSTHSKTETQVDRDDHFDDALESAIKAFQTYYHLKPTGILDAPTATLMSSPRCGVPDNPSVTNNINSHGHSHLNIGTHYIFFPGKPRWPAGQTHLLYSLDSGSHPEAADAVAKAFGTWAAVSHFTFERISDPTVANIKISFQIRDHGDGMPFDGPGGTLAHAAPPTEGIFHFDGDETWAIGAVANSIDLQTVATHEIGHLLGLGHSSVEAAIMYPSISRGATKGLNQDDIDGIRALYTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z7M5</accession>
    <name>A0AA38Z7M5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">WRKY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_019348</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z7M5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.80:FF:000006">
        <property type="entry name" value="WRKY transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.80:FF:000001">
        <property type="entry name" value="WRKY transcription factor 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.80">
        <property type="entry name" value="WRKY domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003657">
        <property type="entry name" value="WRKY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036576">
        <property type="entry name" value="WRKY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044810">
        <property type="entry name" value="WRKY_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31221:SF141">
        <property type="entry name" value="WRKY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31221">
        <property type="entry name" value="WRKY TRANSCRIPTION FACTOR PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03106">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00774">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118290">
        <property type="entry name" value="WRKY DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50811">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="WRKY" evidence="8">
        <location>
            <begin position="227"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="WRKY" evidence="8">
        <location>
            <begin position="409"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="74"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="192"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="267"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="286"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="306"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="341"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9683732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="529" mass="57809" checksum="C36A5AF33C2721C0" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z8U9</accession>
    <name>A0AA38Z8U9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Cucumisin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_016739</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the peptidase S8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z8U9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02120">
        <property type="entry name" value="PA_subtilisin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04852">
        <property type="entry name" value="Peptidases_S8_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.2310:FF:000001">
        <property type="entry name" value="Subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000006">
        <property type="entry name" value="Subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034197">
        <property type="entry name" value="Peptidases_S8_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010259">
        <property type="entry name" value="S8pro/Inhibitor_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045051">
        <property type="entry name" value="SBT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041469">
        <property type="entry name" value="Subtilisin-like_FN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10795">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17766">
        <property type="entry name" value="fn3_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05922">
        <property type="entry name" value="Inhibitor_I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 7" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041244728" description="Cucumisin" evidence="8">
        <location>
            <begin position="23"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="domain" description="Inhibitor I9" evidence="10">
        <location>
            <begin position="32"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="9">
        <location>
            <begin position="131"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="domain" description="Subtilisin-like protease fibronectin type-III" evidence="11">
        <location>
            <begin position="634"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6 7">
        <location>
            <position position="139"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6 7">
        <location>
            <position position="204"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6 7">
        <location>
            <position position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9684424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="733" mass="78559" checksum="2F9DF6FC9CA32232" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z9G1</accession>
    <name>A0AA38Z9G1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_016924</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z9G1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9684695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="95" mass="11004" checksum="52ADE89FE8872F27" modified="2024-01-24" version="1">MFAVTDLNKTSHIQGHLQKTNQKDDGWMNLQKGLELYRIIFMTKTMGSIPSMPLQFNQTASSMRKLKLTLKGFSKKKPYYLSSRRGWLLPPNVKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZB44</accession>
    <name>A0AA38ZB44_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">60S ribosomal protein L31</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_017688</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZB44"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00463">
        <property type="entry name" value="Ribosomal_L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.440.10:FF:000001">
        <property type="entry name" value="60S ribosomal protein L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000054">
        <property type="entry name" value="Ribosomal_eL31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020052">
        <property type="entry name" value="Ribosomal_eL31_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023621">
        <property type="entry name" value="Ribosomal_eL31_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002258">
        <property type="entry name" value="PRK01192.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10956">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10956:SF52">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL31X-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01198">
        <property type="entry name" value="Ribosomal_L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01380">
        <property type="entry name" value="Ribosomal_L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54575">
        <property type="entry name" value="Ribosomal protein L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01144">
        <property type="entry name" value="RIBOSOMAL_L31E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9685746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="121" mass="14063" checksum="2E8577D61AF46CDE" modified="2024-01-24" version="1">MVEKSNKARKEEIVTREYTINLHKRLHGCTFKKKAPKAIKEIRKFAQKAMGTTDVRVDVKLNKHIWSRGIRSVPRRIRVRIARKRNDEEDAKEELFSLVTVAEVPPEGLKGLETKVIDDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZBR9</accession>
    <name>A0AA38ZBR9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">FAD-binding PCMH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_015128</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oxygen-dependent FAD-linked oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZBR9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.43.10:FF:000004">
        <property type="entry name" value="Berberine bridge enzyme-like 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.462.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="entry name" value="Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012951">
        <property type="entry name" value="BBE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32448">
        <property type="entry name" value="OS08G0158400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08031">
        <property type="entry name" value="BBE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041365479" description="FAD-binding PCMH-type domain-containing protein" evidence="8">
        <location>
            <begin position="25"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="9">
        <location>
            <begin position="76"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9686091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="545" mass="60713" checksum="38B9A604BB931B21" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZCZ0</accession>
    <name>A0AA38ZCZ0_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_015470</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O-methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZCZ0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009717">
        <property type="term" value="P:isoflavonoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000213">
        <property type="entry name" value="Coniferyl alcohol 9-O-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000294">
        <property type="entry name" value="O-methyltransferase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016461">
        <property type="entry name" value="COMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001077">
        <property type="entry name" value="COMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012967">
        <property type="entry name" value="COMT_dimerisation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11746">
        <property type="entry name" value="O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08100">
        <property type="entry name" value="Dimerisation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00891">
        <property type="entry name" value="Methyltransf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005739">
        <property type="entry name" value="O-mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51683">
        <property type="entry name" value="SAM_OMT_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="O-methyltransferase dimerisation" evidence="7">
        <location>
            <begin position="31"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="O-methyltransferase C-terminal" evidence="6">
        <location>
            <begin position="143"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR005739-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9686612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="368" mass="40476" checksum="E9A9B35303BC14DF" modified="2024-01-24" version="1">MVGTSETGDVLKVSSEADEAELMLQGQANIWRHMFAFADSMALKCAVELRIADIIHSHARPITLSQIATCIDSPSPDITCLARIMRFLVRAKIFTAAPPPPQSDGGETLYGLTPSSKWLLHDAELSLAPMVFMENHPSLMAPWHCFGTCVKEGGIAFEKAHGRQIWDFASENPEFNKLFNDGMACTAKISIKAVIAAYKDGFGCIGALVDVGGGTGGAVAELVKAYPHIKGINFDLPHVVATAPAYKGVSHVGGDMFESIPYADAIFMKWILHDWNDEDCVKILKNCRKSIPEKTGKVIIVDGVIREDGDEPFDETRLVFDLVMMAHASHGQERTEVEWKKLLEEGGFPRYRILKIPTVQMIIEAYLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZD09</accession>
    <name>A0AA38ZD09_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">BTB/POZ domain-containing protein At3g22104-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_015560</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NPH3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZD09"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043454">
        <property type="entry name" value="NPH3/RPT2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027356">
        <property type="entry name" value="NPH3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32370">
        <property type="entry name" value="OS12G0117600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03000">
        <property type="entry name" value="NPH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51649">
        <property type="entry name" value="NPH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="BTB" evidence="6">
        <location>
            <begin position="5"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="NPH3" evidence="7">
        <location>
            <begin position="193"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="453"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="494"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="454"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9686766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="544" mass="60745" checksum="4F8CF62B33F9571A" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZEN6</accession>
    <name>A0AA38ZEN6_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_016208</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZEN6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9687631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="220" mass="24454" checksum="CCB9736577BF8B17" modified="2024-01-24" version="1">MSDEIAPITLTTLYEMMTDLTRRIERIELILSEYPSSSRGVLGVVRPSSPHLASPTSITLGASHPRPRPCSVLQQHSSYATLATSTRSPSQLRDPPVITVPQERLHPRRRYQRDFSDLGMPLSRVFETFQDMGFLAPLAPRALPDPMPPQFRVDLYCTYHQSAGHHTDRCTALRHAIQDIVDSGTFGHPQSDMLYIPTSARAMHADTASPAVPDLIDLGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZGY5</accession>
    <name>A0AA38ZGY5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 19">Peroxidase</fullName>
            <ecNumber evidence="4 19">1.11.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">PVL29_014344</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="22">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 19">
            <text>2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:56136"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:139520"/>
            <dbReference type="ChEBI" id="CHEBI:139521"/>
            <dbReference type="EC" id="1.11.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16 19">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="16 19">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16 19">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="16 19">Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="19">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peroxidase family. Ascorbate peroxidase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.7" evidence="4 19"/>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZGY5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140825">
        <property type="term" value="F:lactoperoxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00693">
        <property type="entry name" value="secretory_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.420.10:FF:000010">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.520.10:FF:000008">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.420.10">
        <property type="entry name" value="Peroxidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000823">
        <property type="entry name" value="Peroxidase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019793">
        <property type="entry name" value="Peroxidases_heam-ligand_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033905">
        <property type="entry name" value="Secretory_peroxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31235">
        <property type="entry name" value="PEROXIDASE 25-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00458">
        <property type="entry name" value="PEROXIDASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00461">
        <property type="entry name" value="PLPEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00435">
        <property type="entry name" value="PEROXIDASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50873">
        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 16 19" id="KW-0106">Calcium</keyword>
    <keyword evidence="13 18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 19" id="KW-0349">Heme</keyword>
    <keyword evidence="14 19" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="12 16 19" id="KW-0408">Iron</keyword>
    <keyword evidence="8 16 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 19" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6 19" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 19" id="KW-0964">Secreted</keyword>
    <keyword evidence="9 19" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041490067" description="Peroxidase" evidence="19">
        <location>
            <begin position="25"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="Plant heme peroxidase family profile" evidence="21">
        <location>
            <begin position="27"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="142"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="16">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="17">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="37"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="70"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="119"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="198"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU362060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAJ9688640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="328" mass="35194" checksum="58D103AF091E8F96" modified="2024-01-24" version="1">MVQMEPKWLVLLAVILGLSWFAETQQGLKPGFYSSSCPKAEAIVRSTVETHFKEDPTIAAGVLRLHFQDCFVQGCDASILITEGSGERDALPNAGLRGFDVIDDAKTQLEALCPGVVSCADILALAARDAVALSGGANWSVPTGRRDGTNVSSSPDVSNLPAPNDSIPVLRQKFADKGLNTNDLVTLVGAHTIGQANCSSFQYRLYNFTTRGNADPTINPAFLAQLQALCPEGGNGSTRVALDTNSQTKFDVSFFKNVRDGNGVLESDQRLFGDSDTQKIVKNYAGNGRGLLGLRFYFEFPKAMIKMSSIGVKTGTQGEIRKTCSKSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZHQ6</accession>
    <name>A0AA38ZHQ6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Spermidine hydroxycinnamoyl transferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_014607</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9689045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZHQ6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050317">
        <property type="entry name" value="Plant_Fungal_Acyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31642:SF324">
        <property type="entry name" value="SPERMIDINE HYDROXYCINNAMOYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31642">
        <property type="entry name" value="TRICHOTHECENE 3-O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="221"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9689045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="457" mass="51050" checksum="AB1566D5F90535A4" modified="2024-01-24" version="1">MVKLMATYTVRPAKETPGGYRCLSDCDQVKPVNHIPIICFYPPVNMSLESATEILRHSLSEALVIFYPLAGRLHWIGGGRLVLECNALGALLIAVESDAKIDDFGDFRPTQEIQALTPSVDYNKPIHELPLLLVQVTKFRCGGISLGLGTSRIMTDGLSAFPFIQEWAKIARGEQPNSPPFLDRSILLAPEHLTAPVFDHPEYSTQPLLIGKQDNIEERKRESATLALKLSKDQVEKLKDKANKDRSACNNKRPYTRYEAVAGHIWRTACKARQHESQQPTTLSIIVDARNRMEPPLPPRYFGNATFRVTAESTSGELLSKPLGYASGKARQAIEKAKHEYLQSSLVYVKCQEDVTQLRNFHTVGCTEGAFYGNPNLDITSWARLPINGADFGWGQEIYMGPGAIGYDGKVFVFPSRGQDGSFLVVLRLQVRHIDAFQKFFYEDICLQKPLLINPSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZHZ9</accession>
    <name>A0AA38ZHZ9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Prefoldin subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_014718</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the prefoldin subunit beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9689195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZHZ9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009409">
        <property type="term" value="P:response to cold"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23164">
        <property type="entry name" value="Prefoldin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002777">
        <property type="entry name" value="PFD_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20903:SF0">
        <property type="entry name" value="PREFOLDIN SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20903">
        <property type="entry name" value="PREFOLDIN SUBUNIT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01920">
        <property type="entry name" value="Prefoldin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="17"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="75"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9689195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="130" mass="14968" checksum="0549CE4D76418F42" modified="2024-01-24" version="1">MADEANRTAFMEIQGHMIETTGKLKQVQNQMRNKEGEKKRAFLTLEELRQLSDDTNTYKSIGRTRFVLEPKSALMSEQEQKLKDSEVAIAGLQTSKEYLEKQMAEVENNLRELLQQDPGLARQIMSMTVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZJE5</accession>
    <name>A0AA38ZJE5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Xyloglucan endotransglucosylase/hydrolase</fullName>
            <ecNumber evidence="13">2.4.1.207</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_014933</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Secreted</location>
            <location evidence="13">Cell wall</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Secreted</location>
            <location evidence="13">Extracellular space</location>
            <location evidence="13">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="13">Contains at least one intrachain disulfide bond essential for its enzymatic activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the glycosyl hydrolase 16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.207" evidence="13"/>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9689488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZJE5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016762">
        <property type="term" value="F:xyloglucan:xyloglucosyl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042546">
        <property type="term" value="P:cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010411">
        <property type="term" value="P:xyloglucan metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02176">
        <property type="entry name" value="GH16_XET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000025">
        <property type="entry name" value="Xyloglucan endotransglucosylase/hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044791">
        <property type="entry name" value="Beta-glucanase/XTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008264">
        <property type="entry name" value="Beta_glucanase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008263">
        <property type="entry name" value="GH16_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010713">
        <property type="entry name" value="XET_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016455">
        <property type="entry name" value="XTH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31062">
        <property type="entry name" value="XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASE PROTEIN 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00722">
        <property type="entry name" value="Glyco_hydro_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06955">
        <property type="entry name" value="XET_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005604">
        <property type="entry name" value="XET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00737">
        <property type="entry name" value="GLHYDRLASE16"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01034">
        <property type="entry name" value="GH16_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 13" id="KW-0052">Apoplast</keyword>
    <keyword evidence="1 13" id="KW-0134">Cell wall</keyword>
    <keyword evidence="13" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 13" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <keyword evidence="4 13" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041489700" description="Xyloglucan endotransglucosylase/hydrolase" evidence="13">
        <location>
            <begin position="26"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="14">
        <location>
            <begin position="1"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="10">
        <location>
            <position position="102"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="10">
        <location>
            <position position="106"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="12">
        <location>
            <position position="106"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="11">
        <location>
            <position position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR005604-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR005604-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR608264-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9689488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="293" mass="32776" checksum="DDBD94837EEB061D" modified="2024-01-24" version="1">MASFSSTVLLFLISLMAASFIAVSAGTLYEAIDIKWGDERAKILNNGQLLTLSLDRTCGSGFQSKNEYLFGKIEMQLKLVPGNSAGTVTAFYLSSPGPTRDEIDFEFLGNLSGDPYTVHTNVYTQGKGEREQQFHLWFDPTADFHTYSFTWNPKHIIFSVDDTPIREFKNLESIGVQYPKNQAMSLYSSLWDAEDWATRGGLVKTDWSQAPFTASYRNFNANGCIWSAGKSSCSSVSSSLILADNSWLAPELDARSQEKLMWVQRNYMIYNYCTDTKRFPGGFPAECQATSNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZKK7</accession>
    <name>A0AA38ZKK7_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_013126</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZKK7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048316">
        <property type="term" value="P:seed development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00265">
        <property type="entry name" value="MADS_MEF2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1810.10:FF:000009">
        <property type="entry name" value="agamous-like MADS-box protein AGL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050142">
        <property type="entry name" value="MADS-box/MEF2_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033896">
        <property type="entry name" value="MEF2-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002487">
        <property type="entry name" value="TF_Kbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48019">
        <property type="entry name" value="SERUM RESPONSE FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01486">
        <property type="entry name" value="K-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00404">
        <property type="entry name" value="MADSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51297">
        <property type="entry name" value="K_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00350">
        <property type="entry name" value="MADS_BOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="7">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="K-box" evidence="8">
        <location>
            <begin position="88"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="88"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9690813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="227" mass="26285" checksum="A2D1541449962BEA" modified="2024-01-24" version="1">MGRGKIEIKRIENTTNRQVTFCKRRNGLLKKAYELSVLCDAEVALIVFSSRGRLYEYANNSSVKSTIERYKKASADSSNTGSVSEANAQFYQQEASKLHQQIRNLQNSNRHMLGESLGSLNFKDLKSLEIRLEKGISRIRSKKNELLFAEIEYMQKREIDLHNDNQYLRARIAENERNQQQMTLMPGGANYEFMPSQQFDSRNYFQLNGLQPNQSYSRQDQPALQLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZLG8</accession>
    <name>A0AA38ZLG8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transposase-associated domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_013452</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZLG8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029480">
        <property type="entry name" value="Transpos_assoc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13963">
        <property type="entry name" value="Transpos_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase-associated" evidence="1">
        <location>
            <begin position="5"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9691278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="159" mass="18994" checksum="B2E7E871B2936EF2" modified="2024-01-24" version="1">MLIDKSWMQKSRVSSEYHKGVLEFLNFAFSNEPGKEMLSCPCIRWNNCLMQKREIIYDHLLDNGIVRNYFCEPTNTNTNEFDMHDEMQEMLNYAFGMSMPNEESEKSPHVHEEFEKPNEDANFFYNLLREVKHKLYPGCKKFTKLSFIIRLFHMKCLNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZLT8</accession>
    <name>A0AA38ZLT8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Glutamate receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PVL29_012968</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Glutamate-gated receptor that probably acts as non-selective cation channel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZLT8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015276">
        <property type="term" value="F:ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13686">
        <property type="entry name" value="GluR_Plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19990">
        <property type="entry name" value="PBP1_GABAb_receptor_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000172">
        <property type="entry name" value="Glutamate receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000054">
        <property type="entry name" value="Glutamate receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000515">
        <property type="entry name" value="Glutamate receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000188">
        <property type="entry name" value="Glutamate receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044440">
        <property type="entry name" value="GABAb_receptor_plant_PBP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019594">
        <property type="entry name" value="Glu/Gly-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015683">
        <property type="entry name" value="Ionotropic_Glu_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001320">
        <property type="entry name" value="Iontro_rcpt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017103">
        <property type="entry name" value="Iontropic_Glu_rcpt_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18966">
        <property type="entry name" value="IONOTROPIC GLUTAMATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00060">
        <property type="entry name" value="Lig_chan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10613">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037090">
        <property type="entry name" value="Iontro_Glu-like_rcpt_pln"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00079">
        <property type="entry name" value="PBPe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 13" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 13" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="8 13 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 16" id="KW-0732">Signal</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 13" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041230618" description="Glutamate receptor" evidence="16">
        <location>
            <begin position="29"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="576"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="637"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="812"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor C-terminal" evidence="17">
        <location>
            <begin position="454"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="896"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="906"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="923"/>
            <end position="936"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR037090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ9690584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="936" mass="105087" checksum="BD9F414A92CD60C9" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZNM4</accession>
    <name>A0AA38ZNM4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_011474</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZNM4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.430:FF:000003">
        <property type="entry name" value="Probable disease resistance protein At5g66910"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050905">
        <property type="entry name" value="Plant_NBS-LRR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463:SF220">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0611">Plant defense</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="168"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="25"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9692433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="878" mass="101370" checksum="A663A5D0FFBD40CA" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZPV0</accession>
    <name>A0AA38ZPV0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Carbohydrate kinase FGGY C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_011987</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FGGY kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9693075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZPV0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004370">
        <property type="term" value="F:glycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006071">
        <property type="term" value="P:glycerol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046167">
        <property type="term" value="P:glycerol-3-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006641">
        <property type="term" value="P:triglyceride metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10196:SF69">
        <property type="entry name" value="GLYCEROL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10196">
        <property type="entry name" value="SUGAR KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9693075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="135" mass="14918" checksum="9B4CBD374BCCA1C2" modified="2024-01-24" version="1">MLGQACRKGEAQSTYGIEEIIESKHGPLTTLAFKLEGSIAIVGAAYQWLKDSLGIISTASEIKELVAKVDSSGGVYFVPAFNKLSAPWWHDDAHGKRESLLRVDGNATIKNLLMQIQVRVFCYSLIQYLLHCCNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZPX9</accession>
    <name>A0AA38ZPX9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Disease resistance protein RGA3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_011742</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZPX9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14798">
        <property type="entry name" value="RX-CC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038005">
        <property type="entry name" value="RX-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766:SF45">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766">
        <property type="entry name" value="PLANT BROAD-SPECTRUM MILDEW RESISTANCE PROTEIN RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="6">
        <location>
            <begin position="6"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="5">
        <location>
            <begin position="177"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="7">
        <location>
            <begin position="431"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="8">
        <location>
            <begin position="577"/>
            <end position="862"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9692795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="914" mass="103243" checksum="93DC1165490133C4" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZQ29</accession>
    <name>A0AA38ZQ29_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_012067</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9693166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZQ29"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050317">
        <property type="entry name" value="Plant_Fungal_Acyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31642:SF266">
        <property type="entry name" value="HXXXD-TYPE ACYL-TRANSFERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31642">
        <property type="entry name" value="TRICHOTHECENE 3-O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9693166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="456" mass="50175" checksum="935CD11F5D36C2C5" modified="2024-01-24" version="1">MGGGGDFTVTLTKKKVVATMLPMQEHWLSLSNLDLLLPPLDVSVFFCNKNPHDQSVNGGDDIIMAQALVSYYAFSGEVVGNSVGEPELLCNNRVVDFIEAHADVRLEDLNLYNPDVSIEGKLVPKKKNGVLSVQVTELKCGGVVVACTFDHRIADAYSANMFLVSWAEMAQSEPLSVTPSFRRSFLGTRRPGSYVPSFDDMYIPISALTPPKAPQPGADPLMSRLYCVTAEQLSLLQMLATSKSSSCKRTKLESLSAFLWKMVAKSADVDYDNKKICKMGIVVDLRRRLSGGDEDKAAAMANYFGNVLSIPFGEKIIDELKEKPLSWVGDAVHECLEGAVTREHFLGLIDWVEAHRPEPAVAKIYYGGSNDGLAFVVSSGQRFPASKVNFGWGRPALGSYHFPWGGEAGYVMPMPSPVREGDWVVYVHLLKGQIELIEREAAHVFRPFTPEYLKLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZSZ3</accession>
    <name>A0AA38ZSZ3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_010270</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZSZ3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056404">
        <property type="entry name" value="HTH_RNase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001900">
        <property type="entry name" value="RNase_II/R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056403">
        <property type="entry name" value="RNase_II_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050180">
        <property type="entry name" value="RNR_Ribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057324">
        <property type="entry name" value="WH_RNase_II"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23355">
        <property type="entry name" value="RIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23355:SF42">
        <property type="entry name" value="RIBONUCLEASE II, CHLOROPLASTIC_MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23163">
        <property type="entry name" value="CSD_RNase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23161">
        <property type="entry name" value="HTH_RNase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00773">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25255">
        <property type="entry name" value="WHD_RNase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00955">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNB" evidence="1">
        <location>
            <begin position="389"/>
            <end position="685"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9694716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="792" mass="88913" checksum="FD683E168C53188E" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZTY0</accession>
    <name>A0AA38ZTY0_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_010413</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZTY0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000109">
        <property type="entry name" value="POT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11654">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00854">
        <property type="entry name" value="PTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="56"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="164"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="188"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="315"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="348"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="388"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="434"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="476"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="509"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="576"/>
            <end position="595"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9694924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="595" mass="67522" checksum="7A56706E4C1CB1B2" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZVL1</accession>
    <name>A0AA38ZVL1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_008331</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZVL1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006502">
        <property type="entry name" value="PDDEXK-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01615">
        <property type="entry name" value="A_thal_3542"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31579">
        <property type="entry name" value="OS03G0796600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31579:SF14">
        <property type="entry name" value="RNA POLYMERASE SUBUNIT BETA-BETA PROTEIN, PUTATIVE (DUF506)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04720">
        <property type="entry name" value="PDDEXK_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="300"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="342"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="300"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9696017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="400" mass="44677" checksum="C5D2CDC7F37D8EEC" modified="2024-01-24" version="1">MIVMPFPMKIQPIDSYTHEESLRCETVKPVVKSRLKRLLERQFPSVLRINAVEKTGVEESHFSKDGISTEFEPSSVCLAKMVQNFIEESNEKQFPAVRCGRNRCNCFNGNCTDSSEDEFDFYGGGFGDSTLTSSGEACEILKSLVPCASIRERNLLADTAKIVEKNKICKRKDDFCRKLVTDGLLAIGYDASVCKSRWEKSSSYPAGEYEYIDVIVEGERLLIDIDFRSEFEIARSTKSYKAILQTLPQIFVGKPDRLQKIISIVSEASRQSLKKKGMPFPPWRKAEYVKSKWLSPCTRTTPTSILTPTNNSETHPKSDKNQALIPTMSSSGELEIIFRENNSDPDDSMFALSESSGEEEKTASTAVAKQWNPPEIKPKKSLQIAGNIVTGFASVIGDGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A0E5</accession>
    <name>A0AA39A0E5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF3741 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_006991</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9697648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A0E5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032795">
        <property type="entry name" value="DUF3741-assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37897">
        <property type="entry name" value="DNAK FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37897:SF1">
        <property type="entry name" value="DUF3741 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14383">
        <property type="entry name" value="VARLMGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF3741" evidence="2">
        <location>
            <begin position="175"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9697648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="274" mass="30881" checksum="8D1446C819CC42DF" modified="2024-01-24" version="1">MKDLSFFLLKNSLGSKMRRGIRSFCNGDASTSTLNQHKTTPDHGDSSLISSTTLVESPPTLEEMILQLELEEEIARKAKLQEYGEMQRRMSCVNNSDILRSARNALNQYPRFSLDGKDAMYRSSFRNLAPGRKSICCNRGLVRGRCYTDEFDSKLEKKTSSCLPSTLAGESVIWCKPGVVAKLMGLEVMPVPVSCNRSTEKLNSIVNRQNLRRRAQRHEMERRRFVMDMNGSGATQRQGTMASCSKTGRYCVMRPLAVEPANIDGVGWPNRHFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A159</accession>
    <name>A0AA39A159_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_007621</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A159"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9698645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="108" mass="11716" checksum="2FD3DED401157E53" modified="2024-01-24" version="1">MEHQAVIPTTTREKGAILTITPPSYSREISPPSIGNLHSYGASISKKNLILRSVSLINPLLFHLRYSTSAMIITSKGQIPINLTSCSDMQADDSLTSRFPVGLGYVPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A3Z6</accession>
    <name>A0AA39A3Z6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Beta-amylase</fullName>
            <ecNumber evidence="4">3.2.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_005784</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>Hydrolysis of (1-&gt;4)-alpha-D-glucosidic linkages in polysaccharides so as to remove successive maltose units from the non-reducing ends of the chains.</text>
            <dbReference type="EC" id="3.2.1.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.2" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A3Z6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016161">
        <property type="term" value="F:beta-amylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001554">
        <property type="entry name" value="Glyco_hydro_14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31352">
        <property type="entry name" value="BETA-AMYLASE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31352:SF3">
        <property type="entry name" value="INACTIVE BETA-AMYLASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01373">
        <property type="entry name" value="Glyco_hydro_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00750">
        <property type="entry name" value="BETAAMYLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 4" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="516"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="528"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9700115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="542" mass="59931" checksum="B6A0E41C0A6D6BF9" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A5S0</accession>
    <name>A0AA39A5S0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">FAR1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_006737</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A5S0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004330">
        <property type="entry name" value="FAR1_DNA_bnd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46328">
        <property type="entry name" value="FAR-RED IMPAIRED RESPONSIVE (FAR1) FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46328:SF8">
        <property type="entry name" value="PROTEIN FAR1-RELATED SEQUENCE 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03101">
        <property type="entry name" value="FAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAR1" evidence="3">
        <location>
            <begin position="46"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="72"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="141"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="87"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9701508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="208" mass="24051" checksum="D1358E406374B881" modified="2024-01-24" version="1">MQSPTDNETGRMETSAGGELISFEGDTVQEPYEGMLFESEGAAKAFYDEYARRVGFVTRVLSSRKSERDGSIISRGLGCRGGSDNKKTRHLQTQKRDRRREGCTAMILVKREKPGQWIVRKFVRDHNHPLVVQLQKSRPTLDEKDKKIQELTAELRVKKRLTSAYREQLLMFVKDVEDHNEHLSKKVQVVVDNLKDLEAKRQELLLHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A6B0</accession>
    <name>A0AA39A6B0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_006573</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A6B0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000438">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039537">
        <property type="entry name" value="Retrotran_Ty1/copia-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057670">
        <property type="entry name" value="SH3_retrovirus"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648">
        <property type="entry name" value="TRANSPOSASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648:SF28">
        <property type="entry name" value="TRANSPOSON-ENCODED PROTEIN WITH RIBONUCLEASE H-LIKE AND RETROVIRUS ZINC FINGER-LIKE DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25597">
        <property type="entry name" value="SH3_retrovirus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="3">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9701285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="469" mass="52569" checksum="7599C9753E86FFB1" modified="2024-01-24" version="1">MKNRAELFSIFQKFYFEIQTQFNISIRVLHSDNVREYFSAPFTSFMSQHGIIHQSSCAHTPQQNGVAERKNCHLVETARTLLLHSHVPFRFWGDAILIACYLINRMPSSVLHDQIPHSLLFPDQPLYFLPPRVFGCTCFVHILTPGQDKLSARATKCIFLGYSRLQKGYRCYSSETHRNFLSADVTFFEDSPFFSTSESLPISEVLPLPIISPPNAVPSRPLQVYHRRHRVAVPPSLAAVPTDSPPIPSASPAPALPPSADLPIALRKGNRSIHNPHPIYNFLSYHRLSSPYFAFVSAISSVSLPKSTPEALSHPGWRQAMVDEMTALHSNGTWDLVVLPSGKSTVGCRWVYTVKVGPDGQVDRLKARLVAKGYTQVYGSDYGDTFSPVAKIASVRLLLSMTAMRSWPLHQLDIKNAFLHGDLAEEVYMEQPPGFVAQGEFGLVCRLRRSLYGLKQSPRAWFSLFSSVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A6U3</accession>
    <name>A0AA39A6U3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SHSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_004000</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A6U3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.790:FF:000010">
        <property type="entry name" value="17.3 kDa class II heat shock protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031107">
        <property type="entry name" value="Small_HSP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11527">
        <property type="entry name" value="HEAT-SHOCK PROTEIN 20 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="6">
        <location>
            <begin position="40"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9702051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="156" mass="17368" checksum="DDE46100BB0B8412" modified="2024-01-24" version="1">MDLRMIGLDSPLFSALQHMLDAADDSDKSVSAPTRTYVRDAKAMAATPADVKEYPNSYAFIIDMPGLKSGDIKVQVEDDNVLVISGERKREEEKEGAKYVRMERRVGKFMRKFVLPENANTDKISAICQDGVLTVTVEKLPPPEPKKPKTIEVKIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A7F5</accession>
    <name>A0AA39A7F5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Sulfite exporter TauE/SafE family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_004207</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 4-toluene sulfonate uptake permease (TSUP) (TC 2.A.102) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A7F5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031464">
        <property type="term" value="C:Cul4A-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002781">
        <property type="entry name" value="TM_pro_TauE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14255">
        <property type="entry name" value="CEREBLON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14255:SF1">
        <property type="entry name" value="SULFITE EXPORTER TAUE_SAFE FAMILY PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01925">
        <property type="entry name" value="TauE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041330118" description="Sulfite exporter TauE/SafE family protein" evidence="7">
        <location>
            <begin position="23"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="78"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="158"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="259"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="292"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="335"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="376"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="403"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="434"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9702363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="478" mass="52260" checksum="E921DAB01F90F277" modified="2024-01-24" version="1">MAGFGTKWLMLMILCSFMLASAFVSGERSFKHEASTFNVTKEAGFNSNYLSKVVNFLWQSDRSGYQHVWPEMEFGWQIVVGSIIGFFGAAFGSVGGVGGGGIFVPMLSLVIGFDPKSATALSKCMIMGAAGSTVYYNLKLRHPTLDMPIIDYDLALLFQPMLMMGISIGVAFNVVFADWMVTVLLIVLFLGTSTKAFLKGVETWKKETIMKREAAKRLGTNGNGMEEVEYKPLPGGPSNGTQNATNKSKELEVSIIKNVYWKELGLLVFVWVAFLALQIAKNNTATCSMAYWVLNFMQIPVSVGVSLYEAVSLYKGRRIIASKGDAGTNFRVHQLILYCFCGVLAGIVGGLLGLGGGFILGPLFLELGVPPQVSSATATFAMTFSSSMSVVEYYLLKRFPVPYAVYLVFVATIAAFIGQHVVRRLISILGRASLIIFILAFTIFISAISLGGVGISNMIRKIELHEYMGFENLCRYEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A8I4</accession>
    <name>A0AA39A8I4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PB1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_004635</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A8I4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10017">
        <property type="entry name" value="B3_DNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000047">
        <property type="entry name" value="Auxin response factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.330.10">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044835">
        <property type="entry name" value="ARF_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003340">
        <property type="entry name" value="B3_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015300">
        <property type="entry name" value="DNA-bd_pseudobarrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31384">
        <property type="entry name" value="AUXIN RESPONSE FACTOR 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31384:SF3">
        <property type="entry name" value="AUXIN RESPONSE FACTOR 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01019">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101936">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="PB1" evidence="7">
        <location>
            <begin position="668"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="430"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="468"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="758"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="430"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="468"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="488"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="511"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="538"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="765"/>
            <end position="777"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9702963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="795" mass="88351" checksum="0AE5CFE1104F61DE" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A8P1</accession>
    <name>A0AA39A8P1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase Ty1/copia-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_004472</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A8P1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439">
        <property type="entry name" value="GAG-POL-RELATED RETROTRANSPOSON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439:SF502">
        <property type="entry name" value="SECRETED RXLR EFFECTOR PROTEIN 161-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase Ty1/copia-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9702754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="316" mass="36144" checksum="B17CCB48D7CB660D" modified="2024-01-24" version="1">MLVTRSNVRLLVEFKMKMQDIFEMSDLGIMNYFIGMEIYQCRSGIFISQRKYVVDILKKFKLESCKEVATLLAQNEKSSKNDGEKLEEPSAYRSLVGNLLYLTATRPDLMFLAGLLSRFMSSPSNVHMRVAKRVLKYVRGTTNLDIWYLKTREVKLDGYADSDWAGSVYDMKNTFGYVFTIGSGVIYWNSRKQEVVAQSTAEVEYISLVVAANQGIWLRKLLADLGQEQNSPTELYCDNKSAIAIAQNPVQHGRTKHINVKFHSIREVEKNSLVKLHYCSTDVQLADIMTKVLPKSRLEFLRLKLGMSKANLKEEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A9A2</accession>
    <name>A0AA39A9A2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">E3 ubiquitin-protein ligase RMA</fullName>
            <ecNumber evidence="11">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Protein RING membrane-anchor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">RING-type E3 ubiquitin transferase RMA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_004948</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">E3 ubiquitin-protein ligase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 16">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 11">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Endoplasmic reticulum membrane</location>
            <topology evidence="11">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">The RING-type zinc finger domain is responsible for E3 ligase activity.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="11"/>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A9A2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16745">
        <property type="entry name" value="RING-HC_AtRMA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000463">
        <property type="entry name" value="E3 ubiquitin-protein ligase RNF185 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045103">
        <property type="entry name" value="RNF5/RNF185-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12313">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 11" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="13">
        <location>
            <begin position="31"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="85"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU369090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9703371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="226" mass="24694" checksum="E40F66894E626A5F" modified="2024-01-24" version="1">MASGFGESESMSPASAAYGGSGSNDAGDFECNICFELAQDPIVTLCGHLYCWPCLYEWLHHHSQSQECPVCKALIQEEKLVPLYGRGKTSSDPRSKSIPNDSIPSRPAGQRPETAPPVANPFPHLGVGLMGGFAPVRTARFGNFALSAAFGHLSPSLFNIHVHGFPNPRVYGATPGYPFWFSNAMHGGLGHMHRFPHPISQRQRINYHLKNLLFLTGILVILALIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A9E0</accession>
    <name>A0AA39A9E0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_004754</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A9E0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041438523" description="Secreted protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9703114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="71" mass="7793" checksum="4B300EC4BD472483" modified="2024-01-24" version="1">MDLNKRLYYLFLNLTLRFVVMSSSSSSSQTMGGTTDSTPISSSVLVVDRSTLFFSSVLTAEEDEDGEEVEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AA10</accession>
    <name>A0AA39AA10_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_005154</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AA10"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9703746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="102" mass="11099" checksum="61E72579B76E801F" modified="2024-01-24" version="1">MASDLPGEKGRIPTYPSGYNYGKENDINATVELSGEKFKTQSGTASVPPEVTQIHLLNPGPVKKISYRTKFSSTGSSLSLQSNAAGCQVAWKAIHFKEKRLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AFJ5</accession>
    <name>A0AA39AFJ5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Staygreen protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_001923</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the staygreen family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AFJ5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015996">
        <property type="term" value="P:chlorophyll catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024438">
        <property type="entry name" value="Staygreen"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31750:SF4">
        <property type="entry name" value="LP06106P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31750">
        <property type="entry name" value="PROTEIN STAY-GREEN 1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12638">
        <property type="entry name" value="Staygreen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="4" id="KW-0934">Plastid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Staygreen protein" evidence="7">
        <location>
            <begin position="2"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="124"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="128"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9706686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="175" mass="19560" checksum="61E9AB6F9759E8C1" modified="2024-01-24" version="1">MTSKLTLAISQTINNSQLQGWYNRLQRDEVVAEWKKVEDQMSLHVHCHVSGGHFLLDLCAELRYFIFCKALPVVLKAFVHGDSKLFNNYPELQEALVWVYFHSNLPEFNRVECWGQLNNAAAPPPAAAGGGGGGGGVEGRPNMKQVEPSRPEEPSIENMLLPINEPHPMVTRSRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AFM4</accession>
    <name>A0AA39AFM4_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_003426</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AFM4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062064">
        <property type="term" value="F:box C/D methylation guide snoRNP complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000492">
        <property type="term" value="P:box C/D snoRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027921">
        <property type="entry name" value="NOPCHAP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28674:SF1">
        <property type="entry name" value="NOP PROTEIN CHAPERONE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28674">
        <property type="entry name" value="SIMILAR TO DNA SEGMENT, CHR 10, WAYNE STATE UNIVERSITY 102,-EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15370">
        <property type="entry name" value="NOPCHAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9705374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="208" mass="22343" checksum="264087CCA4B08303" modified="2024-01-24" version="1">MGHTSNDLLHLEHKASSSSPLESTLLVCKNGSESKLQKPNHPKGKPITTPLPSSQVLGKVKDFLGVISEANKKLELDAKDNSGKDYDIEVLTGNESEYIEMDLMLGIADLQTPEAVAAAESSIAGFQPVAPLAACSSGTESEDSSDYDDSDNDDDEDSDDDKNDDKNNNKACSVVKHKRSKSAHGDSSMEVPQKNEGSKKRPKIVELS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AG28</accession>
    <name>A0AA39AG28_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Plasma membrane ATPase</fullName>
            <ecNumber evidence="14">7.1.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_002104</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>ATP + H2O + H(+)(in) = ADP + phosphate + 2 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:20852"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Cell membrane</location>
            <topology evidence="14">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIIA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.1" evidence="14"/>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AG28"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008553">
        <property type="term" value="F:P-type proton-exporting transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120029">
        <property type="term" value="P:proton export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000004">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000004">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000211">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006534">
        <property type="entry name" value="P-type_ATPase_IIIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01647">
        <property type="entry name" value="ATPase-IIIA_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42861">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 14" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="11 14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="14" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="62"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="97"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="597"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="625"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="662"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="714"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="749"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="782"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="15">
        <location>
            <begin position="17"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU362083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9706971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="830" mass="91414" checksum="643BE868F7700AFE" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AGK2</accession>
    <name>A0AA39AGK2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_002297</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AGK2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000349">
        <property type="entry name" value="Uncharacterized protein At1g32220, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051207">
        <property type="entry name" value="ComplexI_NDUFA9_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12126">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 39 KDA SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12126:SF5">
        <property type="entry name" value="OS04G0403500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13460">
        <property type="entry name" value="NAD_binding_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-binding" evidence="1">
        <location>
            <begin position="67"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9707241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="294" mass="31664" checksum="E4138885397C377D" modified="2024-01-24" version="1">MLSLSSISPLKISPCRTHFPLPSHHRYSFCRFGVRCSYAEAGVKGDSKSATIDVEADVKSERVVVLGGNGFVGSAICKAAVSKGIEVTSLSRSGRPSQSSSWVDQVNWVTGDVFYVNWDEVLVGATAVVSTLGGFGSEEQMKRINGEANVLAVGAAKDYGVPKFILISVHDYNLPQFLLESGYFTGKRKAESEVLAKYPNSGVVLRPGFIYGKRRVNGFEIPLDLIGEPLERILRATENFTRPLSSLPASDLILAPPVSVDDVALAAVNAITDDDFFGIFTIEQIKEAAAKVSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AHE5</accession>
    <name>A0AA39AHE5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_002306</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AHE5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34222:SF83">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34222">
        <property type="entry name" value="GAG_PRE-INTEGRS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9707254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="300" mass="34116" checksum="9F076F15A9E01DFF" modified="2024-01-24" version="1">MDSAPMCVKFTSTNYSTWVFRFELFLKGKDLWILMARMLKNHPLLTNLRMLGLLLHGLCLMHTSCLSFLPHCSTQSMWAYLKKVYHQDNDARRFQLEHAIAMFQHGSLSIQDYDLAFLTLWHEYADLVTADTIHATTQRDQFLMKLRLKYESVRSSLLNRSHIPSLDICFGELLRKEQRLSTQAILEQSHGNSGTATIAYATQGCGPPMHSKNLQCFCCKEYGHIVATYPKKFCSYCKKKDHIIKKRRINPQNRQAQAFQTSVIVPPVATSAAHDSPSATCSVPAPPAQITAPQKWCNGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AHS4</accession>
    <name>A0AA39AHS4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_002325</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AHS4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14702">
        <property type="entry name" value="bZIP_plant_GBF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045314">
        <property type="entry name" value="bZIP_plant_GBF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044827">
        <property type="entry name" value="GBF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012900">
        <property type="entry name" value="MFMR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45967:SF2">
        <property type="entry name" value="BZIP TRANSCRIPTION FACTOR 68"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45967">
        <property type="entry name" value="G-BOX-BINDING FACTOR 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07777">
        <property type="entry name" value="MFMR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16596">
        <property type="entry name" value="MFMR_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="8">
        <location>
            <begin position="307"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="111"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="307"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="367"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="17"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="126"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="145"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="174"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="187"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="207"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="316"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="369"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="394"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9707292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="414" mass="43788" checksum="BF80DF90B58E84E4" modified="2024-01-24" version="1">MGSSEMDKAGKEKESKTPPTTTQEQSSTTSAGTVNPDWSGFQAYSPIPPHGFLASSPQAHPYMWGVQHLMPPYGTPPHPYVAMYPPGGLYAHPSMPPGSYPFSPFAMPSPNGIAETSGTTPGSMEADGKSSEGKEKLPIKRSKGSLGSLNMLTGKNTELGKTSGASANGVYSKSAESASEGSSEGSDANSQSDSQLKSGCRQDSLEAETSQNGSTCHAPQNGGPNTPHAMVNQTMAIMPISAPGAPGGVPGPTTNLNIGMDYWGAPTSSTIPAMRGKVPSAPVTGGIVTAGSRDSVQSQLWLQDERELKRQRRKQSNRESARRSRLRKQAECDELAQRAEALKEENASLRSEVSRIKSEYEQLLSENASLKERLGEIPGQDDHRSGGRNEQHLGNDTTKQTGQTGQAEHGQGGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AI80</accession>
    <name>A0AA39AI80_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CLAVATA3/ESR (CLE)-related protein 13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_000254</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CLV3/ESR signal peptide family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AI80"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039618">
        <property type="entry name" value="CLE9-13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34359">
        <property type="entry name" value="CLAVATA3/ESR (CLE)-RELATED PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34359:SF28">
        <property type="entry name" value="CLAVATA3_ESR (CLE)-RELATED PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0221">Differentiation</keyword>
    <keyword evidence="4" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="64"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="65"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="97"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9708105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="117" mass="13747" checksum="0D3447C2FAC48C9C" modified="2024-01-24" version="1">MALKRISHLLFVLLWLSILLLFLHEYYNVKPRSISYYSSSSSVSRQPPISRKVLSSKFDFTPFQKQRHRHRHSHHHSHRHGRSPNMQVQPEPAGTEIDPRYGVEKRLVPTGPNPLHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AIT9</accession>
    <name>A0AA39AIT9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_000449</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the Nudix hydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AIT9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034432">
        <property type="term" value="F:bis(5'-adenosyl)-pentaphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008893">
        <property type="term" value="F:guanosine-3',5'-bis(diphosphate) 3'-diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006753">
        <property type="term" value="P:nucleoside phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019693">
        <property type="term" value="P:ribose phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03671">
        <property type="entry name" value="NUDIX_Ap4A_hydrolase_plant_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000032">
        <property type="entry name" value="Nudix hydrolase 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00298">
        <property type="entry name" value="Nudix_RppH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020476">
        <property type="entry name" value="Nudix_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022927">
        <property type="entry name" value="RppH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001936">
        <property type="entry name" value="PRK00714.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001938">
        <property type="entry name" value="PRK00714.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11839:SF30">
        <property type="entry name" value="NUDIX HYDROLASE 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11839">
        <property type="entry name" value="UDP/ADP-SUGAR PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00502">
        <property type="entry name" value="NUDIXFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0464">Manganese</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="8">
        <location>
            <begin position="7"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9708408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="173" mass="19628" checksum="9DE0435B0A7FC4D3" modified="2024-01-24" version="1">MEGLPSGYRPNVGVCLINSDDQVFVASRLNVPGAWQMPQGGIEDGEEPKSAAMRELREETGIVSAEIIAEVPNWLTYDFPPAVKAKVNRLWRGEWHGQAQKWFLMRLTKDDGEVNLASGEAKPEFSEWKWSSPEEVIEQAVDYKRPTYEEVMKTFRPYFSGNANSAKCRSAKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AK96</accession>
    <name>A0AA39AK96_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Homeobox-leucine zipper protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">HD-ZIP protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Homeodomain transcription factor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_000920</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Transcription factor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 10">Belongs to the HD-ZIP homeobox family. Class I subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AK96"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000144">
        <property type="entry name" value="homeobox-leucine zipper protein ATHB-6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045224">
        <property type="entry name" value="HDZip_class_I_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000047">
        <property type="entry name" value="HTH_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003106">
        <property type="entry name" value="Leu_zip_homeo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24326">
        <property type="entry name" value="HOMEOBOX-LEUCINE ZIPPER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24326:SF610">
        <property type="entry name" value="HOMEOBOX-LEUCINE ZIPPER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02183">
        <property type="entry name" value="HALZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00031">
        <property type="entry name" value="HTHREPRESSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 8 9" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="13">
        <location>
            <begin position="81"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="8">
        <location>
            <begin position="83"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="189"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="140"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU369038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9709191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="321" mass="36417" checksum="01CB6DF580680CD0" modified="2024-01-24" version="1">MDGRKLYGGGSNMAVLLRKETLPSPSELLESFWIPDSSTTFHGSKSVVNFEGVGGDDTKDRPFFQTLHEEENGDEDFEGCFHRPEKKRRLTASQVQFLERNFDVENKLEPERKNQLAKDLGLQPRQVAIWFQNRRARFKTKQLEKDYDSLKASYVSLKADYDCILKEKEKLKTELLLLTDKALIGEKEGEKTEALENISPSHAESQKAIPNRVSEHVPPNAPILFCKQEDASSAKSDVFDSDSPLYTDGNHSSLLEPADSSQIFEPDQSEFSQDEEDNLSKSLIPPLCFPKLEDCSYPDPPANACNLGLPAEEQPFWFWSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AKK7</accession>
    <name>A0AA39AKK7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_001041</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 19">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AKK7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13999">
        <property type="entry name" value="STKc_MAP3K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000355">
        <property type="entry name" value="Integrin-linked protein kinase family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000180">
        <property type="entry name" value="serine/threonine-protein kinase STY46-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000312">
        <property type="entry name" value="serine/threonine-protein kinase STY8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF289">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE VIK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="8 13 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 13 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 14" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="67"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="159"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="18"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9709380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="435" mass="49112" checksum="DAB840AF689C4E45" modified="2024-01-24" version="1">MSSRDDNSGGHSSTSAAGKDKASDKQKEKARVSRTSLILWHAHQNDAAAVRKLLEEDQSLVHARDYDSRTPLHVASLHGWIDVAKCLIEFGADVNAQDRWKNTPLADAEGAKKHSMIELLKSYGGLSYGQNGSHFEPKPVPPPLPNKCDWEIDPSELDFSTSSIIGKGSFGEILKACWRGTPVAVKRILPSLSDDRLVIQDFRHEVNLLVKLRHPNIVQFLGAVTDKKPLMLITEYLRGGDLHQYLKEKGSLSPSTAITFAMDIARGMAYLHNEPNVIIHRDLKPRNVLLVNSGADHLKVGDFGLSKLIKVQNSHDVYKMTGETGSYRYMAPEVFKHRKYDKKVDVFSFAMILYEMLEGDPPLSNYEPYEAAKYVAEGQRPMFRAKGYITELRELTEQCWAADMNHRPSFLEILKRLEKIKETLPPDHHWNIFNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AMZ5</accession>
    <name>A0AA39AMZ5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nucleotide exchange factor Fes1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_001512</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9710074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AMZ5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000774">
        <property type="term" value="F:adenyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000431">
        <property type="entry name" value="ARM repeat superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050693">
        <property type="entry name" value="Hsp70_NEF-Inhibitors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013918">
        <property type="entry name" value="Nucleotide_exch_fac_Fes1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19316:SF32">
        <property type="entry name" value="ARM REPEAT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19316">
        <property type="entry name" value="PROTEIN FOLDING REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08609">
        <property type="entry name" value="Fes1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041461691" description="Nucleotide exchange factor Fes1 domain-containing protein" evidence="1">
        <location>
            <begin position="25"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleotide exchange factor Fes1" evidence="2">
        <location>
            <begin position="96"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9710074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="377" mass="42183" checksum="9AAE935B725CF5EC" modified="2024-01-24" version="1">MMGRSASGLALWLLLLASAGAVMAQADRVNKSSSGGLFWSTAKEEGDLMRKSEAQESTVGVDDNADLDGGFSSLDGMLQWAIGRWCFLFFFQLLELMEKLKMPSDAQLMQIAIADLNNSSLPMEDHHRALQELLILVEPIDNANDLNKLGGLTVVIRELDNPDPEIRKISAWILGKASQNNPVVQEQVLELGALQKLMKMVNSSFVEEATKALYAVSALIRKNLAAQELFYAKAGDLMLQDILSNPSIDIRLRRKTVFLVGDLAECQLESTHKAELPFFSNRHFLKSVVDLIASSDLDLQEKALVAVKNLLQLRTTEALVFKDYCDLDGALERMRKQLQQLMEDEYKRDYVMDLESLRREVGLVFHQKLGKVTHVPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D5X6</accession>
    <name>A0AA39D5X6_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_025777</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D5X6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="76"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9672296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="212" mass="24615" checksum="71CDF1F27F1E72AD" modified="2024-01-24" version="1">MELRLEKKCTEELDDALHRAVLGHARFVEHYADLQEKHNELVEKHRNIMEGIAEVKRAAAKAGAKGNGSRFHKYLAAELSTLRREKEREREHLIKENKSLKLQLRDTAEAVHAAGELLVRLREAEGAASVSEDNYNMVQQENERLKKQMEKLKRKHKMEMVTMKQYLAESRLPQSAILNREDSDIAENNMIPTPDDDQAWRAEFGAIYQEHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D9B5</accession>
    <name>A0AA39D9B5_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_021934</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9676658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D9B5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9676658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="133" mass="15312" checksum="05C9EE7637F2B6A2" modified="2024-01-24" version="1">MEDLDSGHLRCEGYGCKKICQRVAQELSHDEPILHEQGSLQLAPHHSKALKIQKQDPTNSRFFRLNRRAGGAHNQECDSNRNQWPLNYVGNNIKIHKGVVVYLLPKTAHQTTRQYSHLAHTTRQYSHLAHTTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D9B8</accession>
    <name>A0AA39D9B8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Probable purine permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_023470</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the purine permeases (TC 2.A.7.14) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D9B8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005345">
        <property type="term" value="F:purine nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015211">
        <property type="term" value="F:purine nucleoside transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030182">
        <property type="entry name" value="PUP_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31376">
        <property type="entry name" value="OS09G0467300 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31376:SF1">
        <property type="entry name" value="PURINE PERMEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16913">
        <property type="entry name" value="PUNUT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="16"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="45"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="112"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="175"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="208"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="254"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="282"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="309"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="332"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU368015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9673942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="351" mass="38941" checksum="80FBDE8F0445DC31" modified="2024-01-24" version="1">MEVEVQLSSNMRKALLVLNCVILSIGNCGGPLVMRLYFVRGGKRIWFSSWLETAGWPLILVPLIITYMHRRTKQGSHAKLFFMKPPLFVASAVIGVLTGLDDYFYAYGVAKLPVSTSALIIASQLAFTAAFAFLLVKQKFTSYSVNAIFLLSIGAGVLALHSSSDRPANESNKEYYLGFFMTLAAAALYGFILPLVELTYKKAKQAITYSLVMEIQMVMCFFATVFCTVGMLVNNDFQAIPKEAKEYELGEAKYYLVVVWNGIIMQCFFLGAIGVIFCASSLVAGIVITVLLPMTEILAVIFFDEKFQAEKGVSLALSLWGFVSYFYGEMKDSKKKKNPTSEKELPQTVNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D9J8</accession>
    <name>A0AA39D9J8_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_024830</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9676031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D9J8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9676031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="59" mass="7069" checksum="2120C8FE8684ACAD" modified="2024-01-24" version="1">MKKLSQPVEEFFYQCEPVDKCSKLVEEFSQSVDGFLTSGYRLRNLFNRLRNVLNRLTFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DA51</accession>
    <name>A0AA39DA51_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_025037</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9676314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DA51"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9676314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="153" mass="16468" checksum="215228C67CDD4F5A" modified="2024-01-24" version="1">MPCLEVLSLNSLSCQLPSLSGLSLLRKLQLDQCNLTPGVIKSDNCLNGLKELSLRNCNLNGGGAFQRILHLSSLEVLNLSGCSPEEGGTLSDIIVGIRHLSNLRALNLSHCKKLPQIPWLPSSLRLLDIHSSIGTSLPPMYSLVNCLKSASQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DAB3</accession>
    <name>A0AA39DAB3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">AIG1-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_024864</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. IAN subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9676075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DAB3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01852">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000840">
        <property type="entry name" value="Immune-associated nucleotide-binding protein 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006703">
        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045058">
        <property type="entry name" value="GIMA/IAN/Toc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903:SF184">
        <property type="entry name" value="GTP-BINDING PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903">
        <property type="entry name" value="GTPASE, IMAP FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04548">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51720">
        <property type="entry name" value="G_AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIG1-type G" evidence="5">
        <location>
            <begin position="17"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="288"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9676075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="351" mass="39573" checksum="46AB7EC9A0B4C73E" modified="2024-01-24" version="1">MGGSSIDDEWEFTAPSSGVRTLVLVGRTGNGKSATGNSILGRKSFKSRASSSGVTSTCELQRTILRDGQIVNVIDTPGLFDLSAESDFVGKEIVKCIDLAKDGVHAVLVVFSVRTRFSKEEEAALHSLQTLFGSKIIDYMIVVFTGGDELEDNDETLEDYLGRECPEPLKEDPLFRLSCTPQTLLLCQNRLVLFDNKTKDEAKKYEQVQKLLSLVNSVILQNGGQPYTDELFMELKKGAQKLRDQTEEVDSLEGYSKREILVLKEQMHKSYEDQLKRITEMVEVKLRETTMKLERQLAEEQAARLKAEEIAQRAQKASNDEIRKLRENLERAQRETEELRKRAESGKCAIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DDU8</accession>
    <name>A0AA39DDU8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Dynamin-related protein 1E</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_019680</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="11">Forms homodimer and may homooligomerize and heterooligomerize to form the phragmoplastin complex. Binds to PHIP1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
            <location evidence="10">Cytoskeleton</location>
            <location evidence="10">Phragmoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DDU8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009524">
        <property type="term" value="C:phragmoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08771">
        <property type="entry name" value="DLP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1240:FF:000009">
        <property type="entry name" value="Dynamin-related protein 1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000228">
        <property type="entry name" value="dynamin-related protein 1E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1240">
        <property type="entry name" value="Dynamin, middle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022812">
        <property type="entry name" value="Dynamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001401">
        <property type="entry name" value="Dynamin_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019762">
        <property type="entry name" value="Dynamin_GTPase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000375">
        <property type="entry name" value="Dynamin_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030381">
        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003130">
        <property type="entry name" value="GED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020850">
        <property type="entry name" value="GED_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566">
        <property type="entry name" value="DYNAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566:SF151">
        <property type="entry name" value="PHRAGMOPLASTIN DRP1E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01031">
        <property type="entry name" value="Dynamin_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02212">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00195">
        <property type="entry name" value="DYNAMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00053">
        <property type="entry name" value="DYNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00302">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00410">
        <property type="entry name" value="G_DYNAMIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51718">
        <property type="entry name" value="G_DYNAMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51388">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6 12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7" id="KW-0505">Motor protein</keyword>
    <keyword evidence="4 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynamin-type G" evidence="14">
        <location>
            <begin position="35"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="GED" evidence="13">
        <location>
            <begin position="527"/>
            <end position="619"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00060413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00065858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9680426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="619" mass="69272" checksum="1720790457632F21" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DEX1</accession>
    <name>A0AA39DEX1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein DETOXIFICATION</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Multidrug and toxic compound extrusion protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_020294</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9681339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DEX1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13132">
        <property type="entry name" value="MATE_eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045069">
        <property type="entry name" value="MATE_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="134"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="200"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="232"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="274"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="348"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="392"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="421"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="453"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9681339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="501" mass="54907" checksum="286D6B8F9FB71989" modified="2024-01-24" version="1">MDSPLFDEDSKLETVDLHHAPTTRLDSDEGDYMPVNNFYEAKAVCCKESAKLWAIAGPIAFNILCNYGTNSFTNIFVGHIGDVELSAVAISLSVIANFSFGFLLGMGSALETLCGQAFGAGQVHLLGVYMQRSWIVLFVACFFILPLYIFATPVLKLLGQEEEIAELAGEFTIQVIPQMFSLAINFPTQKFLQAQSKVGVLAWIGFVALIIHIGVLFLFINVFRWGTAGAAAAYDISAWGVALAQVAYVVGWCKDGWKGLSWLAFRDIWSFVRLSVASAVMLCLEIWYFMTIIILTGHLDEPVVAVGSLSICMNLNGWEGMLFIGVNAAISVRISNELGSGHPRAAKYSVIVTVVESLLIGIFFMAVVMATKNHFAVIFTDTKEMQQAVGKLAYLLGITMVLNSVQPVISGVAVGGGWQALVAYINLFCYYIVGLPLGFLLGYKAKIGVEGIWIGMICGTFLQTLILLFVVWKTNWNKEVEGALERMKKWSGEDEKSIMMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DGJ9</accession>
    <name>A0AA39DGJ9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_018972</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DGJ9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.80">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003441">
        <property type="entry name" value="NAC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036093">
        <property type="entry name" value="NAC_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31719:SF130">
        <property type="entry name" value="NAC DOMAIN-CONTAINING PROTEIN 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31719">
        <property type="entry name" value="NAC TRANSCRIPTION FACTOR 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02365">
        <property type="entry name" value="NAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101941">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51005">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NAC" evidence="5">
        <location>
            <begin position="15"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9683199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="159" mass="17726" checksum="DF93EB2EA1AA1C6F" modified="2024-01-24" version="1">MEKPSFVRSGGMVKLPIGFRFHPTDEELVVHYLKRKALSFPLPASVIPEFNVFLTNPWDLPGDVKERRYFFGSGKGFGNKCRRVTGCGYWKAIGKDIQIVASGSSRAVGMKRTLVFYEGKLGAGTRTQWVMHEYRLVGLGTAPSSTQVQGGIVLNLELW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DKK6</accession>
    <name>A0AA39DKK6_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_015720</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DKK6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9686980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="109" mass="12751" checksum="4E0948F49DB19F3C" modified="2024-01-24" version="1">MDHFFTPNVEMVVQNRKSGKMNQTTINDAYKKEARERVCMLITRWMYEVAIPFNAVTYPSFQPMIEAIGQHSVSMKGPTLHEVRVTNLKKELTLTKDLMKDRMVEWGKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DLF1</accession>
    <name>A0AA39DLF1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FAS1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_014652</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the fasciclin-like AGP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9689108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DLF1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.180.10">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036378">
        <property type="entry name" value="FAS1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000782">
        <property type="entry name" value="FAS1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052806">
        <property type="entry name" value="Fasciclin-like_AGP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33985:SF29">
        <property type="entry name" value="FAS1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33985">
        <property type="entry name" value="OS02G0491300 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02469">
        <property type="entry name" value="Fasciclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00554">
        <property type="entry name" value="FAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82153">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50213">
        <property type="entry name" value="FAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041281016" description="FAS1 domain-containing protein" evidence="3">
        <location>
            <begin position="24"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="213"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="4">
        <location>
            <begin position="44"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9689108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="249" mass="28178" checksum="7E077870596634D5" modified="2024-01-24" version="1">MASKSIILFFILFHFHFLHPTVSEFPTSRATEPETSHEIEVFNIEAISNALSDKGYLSMALSLQLALKSNLIPSEVSDDNTRVTIFCPPNKAFSELKYPQPPLTLLQYHISPSELNREALESSLGPDAKVRTLLAGHSLVITTEPGSRETSINEVKITEWDVYNDGIVIVHAVEDFFDPAFQTLRYPWYDDDNDKVIRNDESGPRQKEDLMGMLLYVVIGALFLGYPQAGILLIIMWFFMPLRLCTCHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DME8</accession>
    <name>A0AA39DME8_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_014783</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9689274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DME8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.40">
        <property type="entry name" value="RutC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035959">
        <property type="entry name" value="RutC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006175">
        <property type="entry name" value="YjgF/YER057c/UK114"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01042">
        <property type="entry name" value="Ribonuc_L-PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55298">
        <property type="entry name" value="YjgF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9689274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="82" mass="9165" checksum="4171E5E5DDAFFE81" modified="2024-01-24" version="1">MGHHKIMFLHSLSSNNAIKANNLVFVSGVLGLIPETGKFISDNVEDQTEQLSLLAIADCYWEDCLRSYKLSFACFVKTHFGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DMF4</accession>
    <name>A0AA39DMF4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_014491</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DMF4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF78">
        <property type="entry name" value="OS12G0587133 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="141"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9688870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="873" mass="100792" checksum="DE28843565EADBBD" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DND6</accession>
    <name>A0AA39DND6_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_012749</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DND6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000001">
        <property type="entry name" value="MYB-related transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051953">
        <property type="entry name" value="Plant_SW-associated_TFs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47997">
        <property type="entry name" value="MYB DOMAIN PROTEIN 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47997:SF28">
        <property type="entry name" value="TRANSCRIPTION FACTOR MYB15-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="14"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="14"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="63"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="63"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="115"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="194"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="251"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="124"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="194"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="251"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9690249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="322" mass="35746" checksum="34754DE51D9A238D" modified="2024-01-24" version="1">MVRTPYYSDVRAQKKGSWSPEEDQKLKAYIKRYGIWNWTEMPKAAGLSRSGKSCRLRWVNYLKPGIKRGNFSCKEEETILEMHKMLGNRWSAIAARLPGRTDNEIKNYWHTKLRKLKDNPAPRTKSQAPKTSTVEAKPIGSPKDASSMGSEHPWSSQLSIDDSSSSIASLAVGADEIQLEGNSGDPMSTQPFAYDSSSSVTSPAVGTEHPWSSQLSIDDSSSSITSLVVGADEIQLEGNSGDPMSTQPFAYDSSSSVTSPAVGTDKNQTMDGSTESERTFGDFQAFPVEDLFMLGDYFWETYPDLEFAFPICEEWVQEPLWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DNR6</accession>
    <name>A0AA39DNR6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase</fullName>
            <ecNumber evidence="3">3.2.2.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_013596</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>NAD(+) + H2O = ADP-D-ribose + nicotinamide + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16301"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57967"/>
            <dbReference type="EC" id="3.2.2.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:16302"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the disease resistance TIR-NB-LRR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.6" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DNR6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061809">
        <property type="term" value="F:NAD+ nucleosidase activity, cyclic ADP-ribose generating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050832">
        <property type="term" value="P:defense response to fungus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043068">
        <property type="term" value="P:positive regulation of programmed cell death"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.430:FF:000002">
        <property type="entry name" value="Disease resistance protein (TIR-NBS-LRR class)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10140:FF:000007">
        <property type="entry name" value="Disease resistance protein (TIR-NBS-LRR class)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045344">
        <property type="entry name" value="C-JID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058192">
        <property type="entry name" value="WHD_ROQ1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017:SF555">
        <property type="entry name" value="TIR-NBS-LRR RCT1-LIKE RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20160">
        <property type="entry name" value="C-JID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23282">
        <property type="entry name" value="WHD_ROQ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0611">Plant defense</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="TIR" evidence="13">
        <location>
            <begin position="11"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00061488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9691466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1006" mass="114374" checksum="81E88DCF39B817DE" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DQP6</accession>
    <name>A0AA39DQP6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DYW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_013070</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. PCMP-H subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DQP6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:002166">
        <property type="entry name" value="Pentatricopeptide (PPR) repeat-containing protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001332">
        <property type="entry name" value="pentatricopeptide repeat-containing protein At5g55740, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000073">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000515">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032867">
        <property type="entry name" value="DYW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF362">
        <property type="entry name" value="DYW DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14432">
        <property type="entry name" value="DYW_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="75"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="176"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="276"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="DYW" evidence="4">
        <location>
            <begin position="491"/>
            <end position="583"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9690727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="583" mass="65071" checksum="03AC5023E0DF9620" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DS25</accession>
    <name>A0AA39DS25_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CRAL/TRIO N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_009854</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DS25"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044834">
        <property type="entry name" value="PATL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45932">
        <property type="entry name" value="PATELLIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45932:SF4">
        <property type="entry name" value="PATELLIN-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03765">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01100">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="CRAL/TRIO N-terminal" evidence="4">
        <location>
            <begin position="13"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9694074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="167" mass="19571" checksum="2F3148FB9F4A9F90" modified="2024-01-24" version="1">MWGIPLLGGDERADVILLKFLRARDFRVADSFHMLEKCLAWRKEFGADEVAEEDLGFKELEGVVAYMHGYDREAHPVCYNAYGVFRDKDMYERIFGDEEKLKKFLRWRVQVLERGIKLLHFKPGGVNSIIQVTDLKDMPKRELRVASNQILSLFQDNYPEMVARKVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DS48</accession>
    <name>A0AA39DS48_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Expansin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_010676</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Causes loosening and extension of plant cell walls by disrupting non-covalent bonding between cellulose microfibrils and matrix glucans. No enzymatic activity has been found.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Secreted</location>
            <location evidence="7">Cell wall</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Membrane</location>
            <topology evidence="7">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the expansin family. Expansin A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DS48"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009664">
        <property type="term" value="P:plant-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22274">
        <property type="entry name" value="DPBB_EXPA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.10:FF:000001">
        <property type="entry name" value="Expansin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.760">
        <property type="entry name" value="Expansin, cellulose-binding-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007118">
        <property type="entry name" value="Expan_Lol_pI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002963">
        <property type="entry name" value="Expansin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007112">
        <property type="entry name" value="Expansin/allergen_DPBB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007117">
        <property type="entry name" value="Expansin_CBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036749">
        <property type="entry name" value="Expansin_CBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009009">
        <property type="entry name" value="RlpA-like_DPBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31867">
        <property type="entry name" value="EXPANSIN-A15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03330">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01357">
        <property type="entry name" value="Expansin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01226">
        <property type="entry name" value="EXPANSIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01225">
        <property type="entry name" value="EXPANSNFAMLY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00837">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49590">
        <property type="entry name" value="PHL pollen allergen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50843">
        <property type="entry name" value="EXPANSIN_CBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50842">
        <property type="entry name" value="EXPANSIN_EG45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0134">Cell wall</keyword>
    <keyword evidence="6 7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041482084" description="Expansin" evidence="7">
        <location>
            <begin position="27"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like EG45" evidence="8">
        <location>
            <begin position="69"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like CBD" evidence="9">
        <location>
            <begin position="188"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9695306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="272" mass="30058" checksum="F727A4BCD8838DE9" modified="2024-01-24" version="1">MALPLRALLSSLLPLLLLLLSHDVHANGWTGNARHVLLSKHHRPKFKPGPWHNAHATFYGGADASQTMGGACGYGDLHKQGYGVQTTALSTVLFNGGQACGACFEIKCVDDPQWCKRGQASLTVTATNLCPPSSNGGWCNPPREHFDLSQPAFLQIAEYKAGIVPVQYRRVPCKKKGGIRFTISGNPYFNLVFIWNVGGAGDITSVQVKGDRKLKWTKLKRNWGQNWETDAMLVGESLTFRVRASDGRYSTSWHIAPKNWQFGQTFEGKNFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DS54</accession>
    <name>A0AA39DS54_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ZF-HD dimerization-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_009565</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9693670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DS54"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006456">
        <property type="entry name" value="ZF_HD_homeobox_Cys/His_dimer"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01566">
        <property type="entry name" value="ZF_HD_prot_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31948:SF171">
        <property type="entry name" value="HOMEOBOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31948">
        <property type="entry name" value="ZINC-FINGER HOMEODOMAIN PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04770">
        <property type="entry name" value="ZF-HD_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51523">
        <property type="entry name" value="ZF_HD_DIMER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZF-HD dimerization-type" evidence="5">
        <location>
            <begin position="70"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9693670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="136" mass="15275" checksum="70801563274AC614" modified="2024-01-24" version="1">MRNSFATTRHYSPSNRRSGMSSPSMFVEEQDQKTLQLSHDLKDKKHKEEHKNAAVFPDKALSSKEKNVIYKECRKNHAASIGGYAVDGCREFMAAGEEGTSASFKCAACSCHRNFHRKEVENDCFCDCSSISTTLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DVZ5</accession>
    <name>A0AA39DVZ5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PRISE-like Rossmann-fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_005553</name>
        <name evidence="3" type="ORF">PVL29_005555</name>
        <name evidence="4" type="ORF">PVL29_005557</name>
        <name evidence="5" type="ORF">PVL29_005559</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9699740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9699742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9699744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9699746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DVZ5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd08948">
        <property type="entry name" value="5beta-POR_like_SDR_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055222">
        <property type="entry name" value="PRISE-like_Rossmann-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32487">
        <property type="entry name" value="3-OXO-DELTA(4,5)-STEROID 5-BETA-REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32487:SF13">
        <property type="entry name" value="LOW QUALITY PROTEIN: IRIDOID SYNTHASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22917">
        <property type="entry name" value="PRISE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRISE-like Rossmann-fold" evidence="1">
        <location>
            <begin position="74"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9699740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9699742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9699744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9699746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="376" mass="42361" checksum="E45AD03BB6AD0681" modified="2024-01-24" version="1">MELHQIPHASVALIVGVTGMAGLSLAEALKKPTALGGPWKVYGAARRPQPTWFPISIVDHYITFDAVSSDDTRAKLSPISSEVTHVFWVAIQVRETEELNITVNAAMLSNVLDVFKSAPSSRLQHVTLQTGTKHYMGPIFDPEKSKQLTAPETPFREDFPRLPFPNFYYALEDLIASYSPSLSYSIHRSSIILGASSRSAYNALLTLAAYAAICKHESLPFRYPGTRYTWEHFCDMSDARLLAEQQIWAGVSEKAKNQAFNCVNGDVFTWKSMWKVVCEVFDVEFVEFDESQEFDFVGLMSGKGKVWESIVKKHGLYESKLEEITCFAALNAVLHFEFQHVCSMNKSRSFGWFGHVDTLQSIGIWVERLRVMNIIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DWM8</accession>
    <name>A0AA39DWM8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Acyl-CoA dehydrogenase IBR3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_005494</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9699653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DWM8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05154">
        <property type="entry name" value="ACAD10_11_N-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000002">
        <property type="entry name" value="Acyl-CoA dehydrogenase fadE12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000018">
        <property type="entry name" value="Acyl-CoA dehydrogenase family member 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041726">
        <property type="entry name" value="ACAD10_11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050741">
        <property type="entry name" value="Acyl-CoA_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002575">
        <property type="entry name" value="Aminoglycoside_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48083:SF13">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48083">
        <property type="entry name" value="MEDIUM-CHAIN SPECIFIC ACYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01636">
        <property type="entry name" value="APH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoglycoside phosphotransferase" evidence="12">
        <location>
            <begin position="42"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="13">
        <location>
            <begin position="552"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="11">
        <location>
            <begin position="664"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="373"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9699653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="820" mass="90924" checksum="B70F7D2FAE2DA8E4" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DXT2</accession>
    <name>A0AA39DXT2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Sec20 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_006241</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the SEC20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DXT2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005606">
        <property type="entry name" value="Sec20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056173">
        <property type="entry name" value="Sec20_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12825">
        <property type="entry name" value="BNIP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12825:SF0">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN SEC20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03908">
        <property type="entry name" value="Sec20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="211"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="Sec20 C-terminal" evidence="12">
        <location>
            <begin position="141"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="76"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF03908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9700823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="289" mass="32315" checksum="D79AA897305F6BFE" modified="2024-01-24" version="1">MDKVTEAVEKAKKDWEETYGKTLDHIKAIEDYGKSSDQKNSLPRLNGLAQDGLALLSSLQFNLDLLAPQLPTDEDIQSANTTLQSWKNQIQSLRSSLRNANLQAKANMRKAAKEERELLLGAGGESTIRRRNLQTKAGMTSAAENITESLRRTRQLMVQEVERNTTMLLTTEESTGVLKKAESEYKGHRSLLMRTRNLLTTMQRQDVLDRIILIVGFVLFSCAVLYVVSKRIGLLKLQRTVTAAIKAGMVRQANIGRDAAEDGINLAPVHDNAIPRVEAPLEQHMHDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DZZ8</accession>
    <name>A0AA39DZZ8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Proteasome subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_004125</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Component of the proteasome, a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="function">
        <text evidence="2">The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of peptide bonds with very broad specificity.</text>
            <dbReference type="EC" id="3.4.25.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the 20S core complex of the 26S proteasome. The 26S proteasome is composed of a core protease (CP), known as the 20S proteasome, capped at one or both ends by the 19S regulatory particle (RP/PA700). The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the proteasome complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the peptidase T1B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DZZ8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03762">
        <property type="entry name" value="proteasome_beta_type_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000035">
        <property type="entry name" value="Proteasome subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000243">
        <property type="entry name" value="Pept_T1A_subB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF0">
        <property type="entry name" value="ATP-DEPENDENT PROTEASE SUBUNIT HSLV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00141">
        <property type="entry name" value="PROTEASOME"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="8 12" id="KW-0647">Proteasome</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0888">Threonine protease</keyword>
    <keyword evidence="9" id="KW-0865">Zymogen</keyword>
    <feature type="active site" description="Nucleophile" evidence="11">
        <location>
            <position position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR600243-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9702234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="231" mass="24919" checksum="FCB488436EBE8084" modified="2024-01-24" version="1">MDLNAPHTMGTTIIGVTYNGGVVLGADSRTSTGMYVANRASDKITQLTDNVYICRSGSAADSQIVSDYVRYFLHQHTIQLGQPATVKVAANLVRLVSYNNKNMLETGLIVGGWDKYEGGKIYAIPLGGTIIEQPFAVGGSGSTYLYGFFDQAWKEGMTKDEAEQLVVKAVSLAIARDGASGGVIRTVIINSEGVTRNFYPGDSLPLWHEELPPQNSLLEVLHSSSPEPMNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E120</accession>
    <name>A0AA39E120_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Eukaryotic translation initiation factor 5A</fullName>
            <shortName evidence="5">eIF-5A</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_004453</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Translation factor that promotes translation elongation and termination, particularly upon ribosome stalling at specific amino acid sequence contexts. Binds between the exit (E) and peptidyl (P) site of the ribosome and promotes rescue of stalled ribosome: specifically required for efficient translation of polyproline-containing peptides as well as other motifs that stall the ribosome. Acts as ribosome quality control (RQC) cofactor by joining the RQC complex to facilitate peptidyl transfer during CAT tailing step.</text>
    </comment>
    <comment type="PTM">
        <text evidence="5">eIF-5A seems to be the only eukaryotic protein to have a hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the eIF-5A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E120"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045901">
        <property type="term" value="P:positive regulation of translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045905">
        <property type="term" value="P:positive regulation of translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04468">
        <property type="entry name" value="S1_eIF5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000012">
        <property type="entry name" value="Eukaryotic translation initiation factor 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000034">
        <property type="entry name" value="Eukaryotic translation initiation factor 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001884">
        <property type="entry name" value="IF5A-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048670">
        <property type="entry name" value="IF5A-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020189">
        <property type="entry name" value="IF5A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019769">
        <property type="entry name" value="Trans_elong_IF5A_hypusine_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00037">
        <property type="entry name" value="eIF_5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11673">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR 5A FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01287">
        <property type="entry name" value="eIF-5a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21485">
        <property type="entry name" value="IF5A-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003025">
        <property type="entry name" value="eIF5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01376">
        <property type="entry name" value="eIF-5a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00302">
        <property type="entry name" value="IF5A_HYPUSINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0385">Hypusine</keyword>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Translation initiation factor 5A C-terminal" evidence="7">
        <location>
            <begin position="85"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9702730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="160" mass="17427" checksum="63054EFF3A2BFE59" modified="2024-01-24" version="1">MSDEEHHFESKADAGASKTYPQQAGAIRKNGYIVIKARPCKVVEVSTSKTGKHGHAKCHFVGIDIFNGKKLEDIVPSSHNCDVPHVTRTDYQLIDISEDGFVSLLTDSGGTKDDLRLPTDDNLLTQIKEGFAEGKDLVVTVMTAMGEEQICALKDIGPKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E1N7</accession>
    <name>A0AA39E1N7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BTB/POZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_001701</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May act as a substrate-specific adapter of an E3 ubiquitin-protein ligase complex (CUL3-RBX1-BTB) which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E1N7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038920">
        <property type="entry name" value="At3g05675-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058039">
        <property type="entry name" value="At3g05675-like_ankyrin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31060:SF7">
        <property type="entry name" value="OS06G0129200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31060">
        <property type="entry name" value="OSJNBA0011J08.25 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25553">
        <property type="entry name" value="BTB-POZ_ANK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="BTB" evidence="4">
        <location>
            <begin position="16"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="At3g05675-like ankyrin-like" evidence="5">
        <location>
            <begin position="211"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9706301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="431" mass="48252" checksum="7B68BBBC9A76B74B" modified="2024-01-24" version="1">MDKAGAAKSCALGDRSTSDITVRLTNREGRPEWFCSHSSILINKSKFFADQLSHLDSTACIEIHCSDFDYDHHVSLLRLLYLPEDSVVDSFDSVKTAIGILQVAVALHCEEITQSCIQYLEAIPWEDKEEEEILKAVSKLGPVAMPIVARLQPVDLSTTRNVFVSAIRFATSIGAPCLPFGDELKTSAQEQVEYMLGEDEDTSLITADDEVKSVVKMGLSQICSSFEKELSSLLLESDLTSEAVEDRVLRSLSDLEWMCNILPKMDLMKEFVSNWTEISGHILTVVEDRKLDSLMWGLKVKLIEVTGKVLEAVGYGNVIFPPPCRVQLLKTWLPYIRKMKPLLDSKGNEDLGFPHKMDEDLCESIEGAIVSLILALPSNDQADFLADWMKTEQVRYPDLSEAFEVWCYRTKSAKRRLVEGLDKVSNATVSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E2V1</accession>
    <name>A0AA39E2V1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Sialate O-acetylesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_003262</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E2V1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052940">
        <property type="entry name" value="Carb_Esterase_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005181">
        <property type="entry name" value="SASA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31988:SF19">
        <property type="entry name" value="9-O-ACETYL-N-ACETYLNEURAMINIC ACID DEACETYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31988">
        <property type="entry name" value="ESTERASE, PUTATIVE (DUF303)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03629">
        <property type="entry name" value="SASA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sialate O-acetylesterase" evidence="2">
        <location>
            <begin position="16"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9705129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="242" mass="26312" checksum="B55ECB86076C13EC" modified="2024-01-24" version="1">MGIASSNQSTENRPSKQIFILSGQSNMAGRGGVNGHHKWDGVVPPECSPDSSILRLNAQLHWESAREPLHADIDTKKACGVGPGMSFANAVRKRVGGTAIKEWARGQPLYENMVNRAKESVKSGGEIKALLWYQGESDTSSYNDAKSYKDNMESLIQNVRQDLGSPSLPIIQVAIASGDSKYMERVREAQKEIDIPNVVCVDAKGLPLKEDHLHLTTEAQVRLGQMLADAYLANFAPPPASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E9V3</accession>
    <name>A0AA39E9V3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_000385</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the DExH box helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E9V3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd17917">
        <property type="entry name" value="DEXHc_RHA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000059">
        <property type="entry name" value="DExH-box ATP-dependent RNA helicase DExH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000480">
        <property type="entry name" value="DExH-box ATP-dependent RNA helicase DExH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000526">
        <property type="entry name" value="DExH-box ATP-dependent RNA helicase DExH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000002">
        <property type="entry name" value="Putative ATP-dependent RNA helicase DHX36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059023">
        <property type="entry name" value="RNA_hel_CTD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF146">
        <property type="entry name" value="DEXH-BOX ATP-DEPENDENT RNA HELICASE DEXH5, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26026">
        <property type="entry name" value="RNA_hel_CTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00358">
        <property type="entry name" value="DSRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50137">
        <property type="entry name" value="DS_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="12">
        <location>
            <begin position="308"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="13">
        <location>
            <begin position="562"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="11">
        <location>
            <begin position="1106"/>
            <end position="1171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="50"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="50"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="77"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00060772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9708307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1195" mass="134837" checksum="C5631F7AE07ABF51" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39EDH3</accession>
    <name>A0AA39EDH3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_001303</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39EDH3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd17917">
        <property type="entry name" value="DEXHc_RHA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000145">
        <property type="entry name" value="probable ATP-dependent RNA helicase DHX40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001388">
        <property type="entry name" value="Probable pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF234">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR ATP-DEPENDENT RNA HELICASE DEAH4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="2" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="14"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="200"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="462"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="650"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="657"/>
            <end position="694"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9709777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="700" mass="78563" checksum="5DEFA9D35F06B87B" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YGK2</accession>
    <name>A0AA38YGK2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_026547</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the AP2/ERF transcription factor family. ERF subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YGK2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009873">
        <property type="term" value="P:ethylene-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000001">
        <property type="entry name" value="Ethylene-responsive transcription factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051758">
        <property type="entry name" value="ERF/AP2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31657">
        <property type="entry name" value="ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR ERF061"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31657:SF73">
        <property type="entry name" value="OS02G0752800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00367">
        <property type="entry name" value="ETHRSPELEMNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0936">Ethylene signaling pathway</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="10">
        <location>
            <begin position="143"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="284"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="284"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9670054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="340" mass="37638" checksum="002956CDA6B30C05" modified="2024-01-24" version="1">MAAAIDIYSSSRAVFSEREELMKALEPFMKASSQPNMYLDFCSTSTTHMFSQGFSSYDQMGLEQTGSIGLNHITPAQILQIQAQIQFQQQQQQRMAAVATASSIQNQRLSQWQQQQQQHTLNFLGPKAIPMKQVGTPPKPAKLYRGVRQRHWGKWVAEIRLPKNRTRLWLGTFDTAEEAALAYDKAAFKLRGEFARLNFPNLRHQGSLVAGEFGDYKPLHSSVDAKLQAICQNLAISQKQGNSGKPGLVSDAKIESSTHQAEMVLDNSSDPESGFLGLEDCKVEISSSSSSPSPSDESSAGSSSPESDISFLDFTDSQWNDSECLTLEKFPSVEIDWASI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YH72</accession>
    <name>A0AA38YH72_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="ORF">PVL29_026739</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="26">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RLP family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="26">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YH72"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905393">
        <property type="term" value="P:plant organ formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009791">
        <property type="term" value="P:post-embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048608">
        <property type="term" value="P:reproductive structure development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048367">
        <property type="term" value="P:shoot system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000453">
        <property type="entry name" value="Leucine-rich receptor-like protein kinase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000275">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000632">
        <property type="entry name" value="leucine-rich repeat receptor-like protein kinase TDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000530">
        <property type="entry name" value="receptor protein-tyrosine kinase CEPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000215">
        <property type="entry name" value="Receptor-like protein kinase HSL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051716">
        <property type="entry name" value="Plant_RL_S/T_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48053">
        <property type="entry name" value="LEUCINE RICH REPEAT FAMILY PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48053:SF159">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16 23" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="20" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="18 24" id="KW-0472">Membrane</keyword>
    <keyword evidence="14 23" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-0675">Receptor</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <keyword evidence="11 24" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="17 24" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="636"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="25">
        <location>
            <begin position="694"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="723"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EMBL" id="KAJ9670384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="991" mass="109822" checksum="BF4ACD401E04DCBA" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YHI8</accession>
    <name>A0AA38YHI8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Lysine-rich arabinogalactan protein 18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_026839</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YHI8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044981">
        <property type="entry name" value="AGP9/17/18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37209:SF3">
        <property type="entry name" value="LYSINE-RICH ARABINOGALACTAN PROTEIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37209">
        <property type="entry name" value="LYSINE-RICH ARABINOGALACTAN PROTEIN 17-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041244726" description="Lysine-rich arabinogalactan protein 18" evidence="2">
        <location>
            <begin position="22"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="23"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="167"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="179"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9670547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="241" mass="22848" checksum="745945688127EC58" modified="2024-01-24" version="1">MDRSSAFGLAFICIIVAGVGGQSPAASPTATPVTAHSPATPAVATPPASSPAAAAPTKSKTPAPVTAPTSSPPTAVSSPPASSPVSSPPTPAVATPALSPAASSPVAVTPVSSPPAPVPVSSPPLPSPANSPPVPAPANPPAASPPAPVSSPPAEAPAAEVPSPAPSKKKTKKKLKSPAPAPAPEALSPPAPPTEAPGPGLDATSPSPSLPDESGAEKITCVMQKVVGCLALGWAALGLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YJH2</accession>
    <name>A0AA38YJH2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Signaling peptide TAXIMIN 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_025332</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YJH2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055283">
        <property type="entry name" value="TAXIMIN_1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33834">
        <property type="entry name" value="SIGNALING PEPTIDE TAXIMIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33834:SF4">
        <property type="entry name" value="SIGNALING PEPTIDE TAXIMIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9671570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="73" mass="7885" checksum="8B5D9F4A4091C3A2" modified="2024-01-24" version="1">MEDCRPLGFLIGLPFAFLALILSVVGAIVWILGTVLSCLCPCCICCAGLANLAVSLVKMPIKVMRWFIDQIPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YQX7</accession>
    <name>A0AA38YQX7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myb/SANT-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_024073</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YQX7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045026">
        <property type="entry name" value="LIMYB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024752">
        <property type="entry name" value="Myb/SANT-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47584">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47584:SF9">
        <property type="entry name" value="L10-INTERACTING MYB DOMAIN-CONTAINING PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12776">
        <property type="entry name" value="Myb_DNA-bind_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb/SANT-like" evidence="2">
        <location>
            <begin position="18"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="Myb/SANT-like" evidence="2">
        <location>
            <begin position="166"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="Myb/SANT-like" evidence="2">
        <location>
            <begin position="323"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="490"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9674942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="621" mass="72274" checksum="AE0A3A084218935C" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2025-06-18" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YR11</accession>
    <name>A0AA38YR11_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">ABC transporter B family member 13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_024124</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015421">
        <property type="term" value="F:ABC-type oligopeptide transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090374">
        <property type="term" value="P:oligopeptide export from mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18577">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18578">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03249">
        <property type="entry name" value="ABC_MTABC3_MDL1_MDL2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000205">
        <property type="entry name" value="ABC transporter B family member 4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394:SF11">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="47"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="95"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="192"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="274"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="310"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="684"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="727"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="809"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="913"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="947"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="50"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="373"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="686"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="1008"/>
            <end position="1244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="617"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="623"/>
            <end position="642"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9675035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1300" mass="141765" checksum="3E5DAAD505BC5CFC" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YRI2</accession>
    <name>A0AA38YRI2_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_024326</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YRI2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9675330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="179" mass="19396" checksum="5BDBF4F7F6920286" modified="2024-01-24" version="1">MSFKARNEIGNEVLVPKCISALLLILDTLLQSRSKFSSETTEGNAVGFVLDSTGEHASLSIPPNVENKLASNAHEKELDSALEKILGKSTGYLTIKESRRVLLVACELLKQQVPAVVMQAGLQLCARLTKTHSLALEFLENGDTSNNYGVGDTTNSKWKPTCWPCAPSSLFNINGNGYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YSG1</accession>
    <name>A0AA38YSG1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Plastocyanin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_024616</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the multicopper oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YSG1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033138">
        <property type="entry name" value="Cu_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002355">
        <property type="entry name" value="Cu_oxidase_Cu_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF487">
        <property type="entry name" value="LACCASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00079">
        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00080">
        <property type="entry name" value="MULTICOPPER_OXIDASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0186">Copper</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Plastocyanin-like" evidence="8">
        <location>
            <begin position="37"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9675769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="175" mass="19468" checksum="1DB41DAB1BB80CF9" modified="2024-01-24" version="1">MASLRSLADAKHPCNVPLSMSTKLIYTLSINSYPCINNSCSGANGTRAGTEVRVLEYNSTVEIVFQGTNLVQGVTHPIHLHGHSFYVVGWGFGNFDENRDPLHYNLVDPPHQSTISVPQNGWTTIRFEASNPGVWFMHCHVERHQTWGMETAFIVKNGKHPEAQMLPPPSDMPPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YSV2</accession>
    <name>A0AA38YSV2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">MICOS complex subunit Mic10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_024750</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MICOS complex subunit Mic10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YSV2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061617">
        <property type="term" value="C:MICOS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007512">
        <property type="entry name" value="Mic10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21304">
        <property type="entry name" value="MICOS COMPLEX SUBUNIT MIC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21304:SF0">
        <property type="entry name" value="MICOS COMPLEX SUBUNIT MIC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04418">
        <property type="entry name" value="DUF543"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9675928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="101" mass="10908" checksum="8D58B0141444C2A7" modified="2024-01-24" version="1">MAENKQIPPPYDLDAKWDACLDLTVRRFVYSSFAGAFGGLLLFRSPVTRWASVAFGAGLGLGSAYTECSQKFGGYPARFLPPKISDAPASQWIGLCSKGQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YVH2</accession>
    <name>A0AA38YVH2_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_022277</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YVH2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035251">
        <property type="term" value="F:UDP-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000064">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48047">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48047:SF118">
        <property type="entry name" value="HEXOSYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9677189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="459" mass="50483" checksum="F9887E7AEB7AC912" modified="2024-01-24" version="1">MSTTAAAHVLLYPFTSSGHIIPILDLATKLLSRDLDVTVLVSPSNLPLLDSLLSKYPSSFQSLVLPLPELAVVTPTNLVFNLRAMSGLSDDIIQWFHSHPNPPVAIVSDFFLGWTHKIACQLGVSHIVFSPSGVLALSVSYTVWRDRPKNDEPENQDFMVSFPSIPNSPSYPWWQISHLYRNREDGDPDKEYLRNCMLDNIGSWGLVVNTFTELERVYIEAMKKLMGHNRVWAVGPLLPAPEDDDAKRGGSSAVPSHKVLSWLDQCQNDSVVYICFGSRTSLPNQQMVVLAAALEASGVNFIWCVRQQGKGAVASESGVIPEGFEDRVGNRGFVIRGWAPQVQILRHRAVGAFLTHCGWNSTLEGLAAGVVMLTWPMGADQYTNANLLVDEVGVGIRVAEETRRVPDSTELARILSEAVDGSRPEKVRAMELRDAALSAANGGSSDRDLDDLVERLKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YW11</accession>
    <name>A0AA38YW11_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_022457</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YW11"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33133">
        <property type="entry name" value="OS08G0107100 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33133:SF3">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="131"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="169"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="223"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="265"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9677474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="326" mass="36141" checksum="1C8D79306194718B" modified="2024-01-24" version="1">MERTGRIMRRSVYTFLQQYQFFTSTAAPLVLPFSASILLSQAFLPFSLPFLPEIHDRLQFLFKAAGFPPSSQFFTILNLKLSQTISSSIFTLPFTISFLLIGKACIIQALNHNKPGFPPSFSSFRSLYCPLLITYIWNSLLILSANATAFSVLFIGFNCLEGLGYSSPNFVLFLSAAAAILYSIILANSLIICNLALVLSGMERCGGPIAILKACVMIRGRTSTALSLALPINLALAAVEALFQYRVVRAYHFSGKPRSSIALEGLFIAYMYSFLVVLDTIVSCIFFKRCKRGCWTDQDGTHSYRIEIAEEGGKAFENTRISEELP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YWF9</accession>
    <name>A0AA38YWF9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_022649</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">The B regulatory subunit may modulate substrate selectivity and catalytic activity, and may also direct the localization of the catalytic enzyme to a particular subcellular compartment.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the phosphatase 2A regulatory subunit B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YWF9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000609">
        <property type="entry name" value="Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000009">
        <property type="entry name" value="PP2A_PR55"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11871">
        <property type="entry name" value="PROTEIN PHOSPHATASE PP2A REGULATORY SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037309">
        <property type="entry name" value="PP2A_PR55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00600">
        <property type="entry name" value="PP2APR55"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5 6" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="122"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU331113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9677792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="507" mass="57611" checksum="E8755FD55DEE3B92" modified="2024-01-24" version="1">MALCTDSSSSLQWKFSQAFGERAPGEDVQDIDMISAMEFEKSGDYLAVGDRGGRVVVFERKDEKDSLNQWHSRNELEQLDFTTMQHPIFHYKTEFQSHEPEFDYLRSLEIEEKINKVKWCATPNGSLFILSTNDKTIKLWKVKDHKPKKVKELNSNPFVHSENVLLAESSFVSGQDKPSVANGYALEWTEKIDNMSSSQDRHAKRANIGETVSARCRKVYAHAHDYNINSISNNSDGETFVSADDLRINLWNLEISDQCFNIIDMKPSNMEDLTEVITAAEFHPIHCNLLAYSSSRGFIRLVDLRQSAICDHQARILQHGESSGLKSFFTEIVSSISDIKFASDGRHLLSRDYMNLKLWDIRMETSPVAIFKIHEHLRPKLCDLYNNDAIFDKFECCLSGYGLHFATGSYSNLLRIFSYGVGSEEGITMEASRNPNRKSHLQAAPRARRSSLSNLTRGFSRQANENWSSGSNELSCNLSSKLLHLAWHPTTNFIACAAGNSLYMYYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YWV1</accession>
    <name>A0AA38YWV1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_022782</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9678002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YWV1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005162">
        <property type="entry name" value="Retrotrans_gag_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33223:SF10">
        <property type="entry name" value="AMINOTRANSFERASE-LIKE PLANT MOBILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33223">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03732">
        <property type="entry name" value="Retrotrans_gag"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Retrotransposon gag" evidence="2">
        <location>
            <begin position="254"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="380"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="388"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9678002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="437" mass="49460" checksum="05505BC295063C17" modified="2024-01-24" version="1">MSTPSRSLSSARGEEENYFEWRRAMERRQLESERQMQTLLQETAKLKEENAVLRIQASSTGPPCGQHSRGQEANSRPNPESIYPRTAGAIPETYNVRPQEPCTPAHQAPQEESSDSTRLSSKRQRDKRPQLLGAMRARLGPQEPGKTKPPAATTWGAHPDPLVTPMVPNAFPHQAIRQVGRNHPNEPPTGSISKRLDDMLPTPFCSHIIHYEPPRGFLVPKFSTYDGSSDPFDHIMHYRQLMTLDIGNDALLCKVFPASLQGQALSWFHCLPPNSVDNLRDLSEAFVGQYLCSARHKQNISTLQNIKMQESESLREFVKRFGQVVLQVEACSMDAVLQIFKRSICPGTPFFESLTKKPPTTMDDLFRRASKYSSLKMTYEQPPSKSWLPDRHPGVVRKEMPNFRTDQGRSVEGKKNRAARKGRPSRPSPYPMRSFSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YYF2</accession>
    <name>A0AA38YYF2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_020994</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YYF2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009791">
        <property type="term" value="P:post-embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000233">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase TDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000111">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001347">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001488">
        <property type="entry name" value="Receptor-like protein EIX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046956">
        <property type="entry name" value="RLP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063">
        <property type="entry name" value="LRR RECEPTOR-LIKE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063:SF101">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE FLS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041459291" description="Leucine-rich repeat-containing N-terminal plant-type domain-containing protein" evidence="13">
        <location>
            <begin position="26"/>
            <end position="1140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1081"/>
            <end position="1104"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="14">
        <location>
            <begin position="36"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="15">
        <location>
            <begin position="259"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9678949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1140" mass="125613" checksum="A3CEDACD5FBCCF94" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YZS6</accession>
    <name>A0AA38YZS6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_021365</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YZS6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.60.10:FF:000091">
        <property type="entry name" value="Zinc finger CCHC-type-containing 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025836">
        <property type="entry name" value="Zn_knuckle_CX2CX4HX4C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47798:SF2">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47798">
        <property type="entry name" value="OS04G0555800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14392">
        <property type="entry name" value="zf-CCHC_4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="7">
        <location>
            <begin position="130"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="7">
        <location>
            <begin position="157"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="245"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="12"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="34"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="45"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="245"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="262"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9679409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="279" mass="30975" checksum="336EDAE23CDB5EDB" modified="2024-01-24" version="1">MVSQRQRLARKIYKEAHPELFPKSEPAAPQDPTKKKKKKSKLRPKRAEMKGPKSTEKSDKSKLRKHPLRVPGMKPGESCFICKAKDHIAKHCPEKAQWERHKICLLCRQRGHSLKNCPDKGEEKLDKKLCYNCGETGHSLANCPQPLQEGGTKFASCFICNERGHLSKNCPKNSHGIYPKGGCCKICGGVTHLAKDCPNKGCNNGPVAAIAGGIEHEERPKVTKFVSGDDLDDDFMVADTYTGYKEKSSEAKVHPTPESKNSHVKPKKKQGPKVVNFVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z233</accession>
    <name>A0AA38Z233_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_020062</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z233"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000010">
        <property type="entry name" value="Transcriptional activator Myb isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000016">
        <property type="entry name" value="Transcriptional activator Myb isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF232">
        <property type="entry name" value="TRANSCRIPTION FACTOR MYB3R-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="43"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="48"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="95"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="95"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="147"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="151"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="416"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="482"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="527"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="28"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="433"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="482"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="527"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="540"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9680961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="599" mass="66569" checksum="6638089B7127DEA7" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z2K3</accession>
    <name>A0AA38Z2K3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">3-ketoacyl-CoA synthase</fullName>
            <ecNumber evidence="10">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_020111</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a very-long-chain acyl-CoA + malonyl-CoA + H(+) = a very-long-chain 3-oxoacyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:32727"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:90725"/>
            <dbReference type="ChEBI" id="CHEBI:90736"/>
            <dbReference type="EC" id="2.3.1.199"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the thiolase-like superfamily. Chalcone/stilbene synthases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="10"/>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9681047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z2K3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009922">
        <property type="term" value="F:fatty acid elongase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00831">
        <property type="entry name" value="CHS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000028">
        <property type="entry name" value="3-ketoacyl-CoA synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012392">
        <property type="entry name" value="3-ktacl-CoA_syn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013747">
        <property type="entry name" value="ACP_syn_III_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013601">
        <property type="entry name" value="FAE1_typ3_polyketide_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31561">
        <property type="entry name" value="3-KETOACYL-COA SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08541">
        <property type="entry name" value="ACP_syn_III_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08392">
        <property type="entry name" value="FAE1_CUT1_RppA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036417">
        <property type="entry name" value="3-ktacl-CoA_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="56"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="90"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="FAE" evidence="13">
        <location>
            <begin position="112"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-ketoacyl-[acyl-carrier-protein] synthase III C-terminal" evidence="14">
        <location>
            <begin position="417"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="255"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="334"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="418"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="422"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="451"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR036417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR036417-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9681047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="530" mass="59018" checksum="FF70EDEDA6155C94" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z4F7</accession>
    <name>A0AA38Z4F7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_018005</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9681912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z4F7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF78">
        <property type="entry name" value="OS12G0587133 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="207"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9681912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="939" mass="107893" checksum="8B93160A4A5B5BC5" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z566</accession>
    <name>A0AA38Z566_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_018500</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z566"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47871">
        <property type="entry name" value="NAC DOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47871:SF2">
        <property type="entry name" value="OS03G0221300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9682593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="708" mass="78992" checksum="180D41233FCEC0D1" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z5H3</accession>
    <name>A0AA38Z5H3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase Ty1/copia-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_018369</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z5H3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439">
        <property type="entry name" value="GAG-POL-RELATED RETROTRANSPOSON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439:SF486">
        <property type="entry name" value="RLK (RECEPTOR-LIKE KINASE) PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase Ty1/copia-type" evidence="2">
        <location>
            <begin position="179"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9682438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="544" mass="63251" checksum="648DBC6C4D4A6A71" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z850</accession>
    <name>A0AA38Z850_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box associated beta-propeller type 3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_019293</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z850"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013187">
        <property type="entry name" value="F-box-assoc_dom_typ3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017451">
        <property type="entry name" value="F-box-assoc_interact_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01640">
        <property type="entry name" value="F_box_assoc_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31111">
        <property type="entry name" value="BNAA05G37150D PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31111:SF136">
        <property type="entry name" value="F-BOX ASSOCIATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08268">
        <property type="entry name" value="FBA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box associated beta-propeller type 3" evidence="1">
        <location>
            <begin position="48"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9683654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="144" mass="16905" checksum="085ED3D7A0A4A60E" modified="2024-01-24" version="1">MVNNNILPDDLIVEILLRLPAWYAVIRDPVFMAKYLDHQANFSDDEYSFAMLSNETLDNVSTRIHISIFRDSHYRFPLVYGPCNGILCPYSPKGLTLWNPATREIKSLPRTTFRHSEFWECAFTFTTFGHDPKTDDYKIFRFLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z8N6</accession>
    <name>A0AA38Z8N6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SHSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_016751</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z8N6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd06472">
        <property type="entry name" value="ACD_ScHsp26_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.790:FF:000009">
        <property type="entry name" value="17.6 kDa class I heat shock protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031107">
        <property type="entry name" value="Small_HSP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11527">
        <property type="entry name" value="HEAT-SHOCK PROTEIN 20 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="6">
        <location>
            <begin position="37"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9684441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="151" mass="17051" checksum="DE39B0CC41B02093" modified="2024-01-24" version="1">MSLIPSFFGGRRNNMFDLWDPFQDFPFTGGALSVPGETASFASTRIDWKETPEAHVFKADLPGVKKEEVKVEVEEGRILQISGDRSVEKEEKNDKWHRVERSSGKFMRRFRLPENVKVDEVKAAMENGVLTVTVPKAEVQKPDVKAIDISG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZA23</accession>
    <name>A0AA38ZA23_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mediator complex subunit 15 KIX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_017284</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZA23"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.246.20:FF:000003">
        <property type="entry name" value="Mediator of RNA polymerase II transcription subunit 15a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.20">
        <property type="entry name" value="Coactivator CBP, KIX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036529">
        <property type="entry name" value="KIX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036546">
        <property type="entry name" value="MED15_KIX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044661">
        <property type="entry name" value="MED15a/b/c-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33137">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 15A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33137:SF4">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 15A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16987">
        <property type="entry name" value="KIX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47040">
        <property type="entry name" value="Kix domain of CBP (creb binding protein)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mediator complex subunit 15 KIX" evidence="3">
        <location>
            <begin position="36"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9685191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="147" mass="16518" checksum="0B673A203C3524AD" modified="2024-01-24" version="1">MAFPIGKAGVSLRLSSHSTCSQFQRPAQAELVMDGSDWETHLSRDSRLRIVNRIMATLRRHLPVSGPEVLHELWKIADRFEEKIYSAATSESDYLRKISLKMLAIETNCFNAATNSLPSNSAAYLKKSSYGKKSPAMKESHRSSKFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZAZ3</accession>
    <name>A0AA38ZAZ3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_017638</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZAZ3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19446">
        <property type="entry name" value="REVERSE TRANSCRIPTASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="254"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9685680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="363" mass="42191" checksum="5AFC8EE2E068F040" modified="2024-01-24" version="1">MWLEEKGFMDQMKRWWGSLSFTGSFSFVLDAKLRALKDILKSWNKEVFGLIESKKREALGQVEHWDEVEKHSPLSLEDCEARKEAQEAYKSWVLREEISWRQKSRELWLKEGDNNTRFFHRMANFHFRRNWPSKLKVNGCWHTEENELKSSVVGAFKALYTEEGGWRPSVEGLSLRRLASYEAEGLETPFLEGEVYAALSNLGKDKAPGPDGFTMAFWMFCWDVVKMEMMGFFMEFHERGRFVKSLNATFLVLVPKKGGAEDLKDFRPISLVGSLYKLLAKVLANRIKKVMGKVISESQNAFVEGRQILDAVLIANEVVDSRLKDEEGGVLCKLDIEKTYDHVCWKFLLAVLKKMGFRERWLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZB75</accession>
    <name>A0AA38ZB75_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Disease resistance RPP13-like protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_017308</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056789">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766:SF40">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766">
        <property type="entry name" value="PLANT BROAD-SPECTRUM MILDEW RESISTANCE PROTEIN RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25019">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0611">Plant defense</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="7">
        <location>
            <begin position="6"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="6">
        <location>
            <begin position="185"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="8">
        <location>
            <begin position="445"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="domain" description="R13L1/DRL21-like LRR repeat region" evidence="9">
        <location>
            <begin position="710"/>
            <end position="839"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF25019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9685229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1669" mass="188395" checksum="C71FEECAAEE2104F" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZE78</accession>
    <name>A0AA38ZE78_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_015949</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZE78"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9687272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="29" mass="3353" checksum="12A82558811EAB8F" modified="2024-01-24" version="1">MADTNSTDQQELQAQLFFHLISKDDKKVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZEC1</accession>
    <name>A0AA38ZEC1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_015611</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZEC1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051504">
        <property type="entry name" value="Plant_metabolite_acyltrans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31625">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9686834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="469" mass="52288" checksum="C4776DD36BED525D" modified="2024-01-24" version="1">MDVNDSTLSCHMASTEMVTVLEEFRVSPPSSTAVDEKYLPLTFFDIPWIHYHLVQSVFFYEYPHPKTHFIETTIPALKHSLSLTLKYFYPFAGNLLFPPNLGKPQIHYADGDSVSLTFVESSDDFSYLTANHQRDVARFHPLIPQMPPVSVSNDTLVAPLVAIQVTLFPNSGISFGFAANHVVADGNTFIRFVRLWASINKLGEETMTLEGAILPFYDRTMVKDSNGVGSIFWQHIGKKKFVGYQPQLPTNNVLGTFLLSQAHLQRLKRWVMVQCPKIPHVSSFTVACGYTWACVAKARARSSDKVGENELQHFTFPVDCRARLNPPLPENYFGNCLAPCLVAIKSTQLIDDEDGFVAAVNAMGEALGKTLQNEEGVLRGAEKWISDLESRNKERNLGAAASPKFAVYNIDFGFGRLQKREMISIDVTNSISLHEGKTNMGDIEVGVTLPKMTMDVFASIFANGLKVYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZEF4</accession>
    <name>A0AA38ZEF4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_016115</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZEF4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052751">
        <property type="entry name" value="Plant_MAPKKK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48011">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTIONAL COMPLEX SUBUNIT CAF120-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48011:SF18">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 19-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9687501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="113" mass="12512" checksum="D9F85FA92510EC62" modified="2024-01-24" version="1">MEVDGSLVYNLILEYAPGEALCYVHERGVVHCDLKPDNVRVFPGGDGGKIADVGLATRVGEQEKCMGSGSRARRLTCRQSFWLCMSMGAPIDMWSLGCTVVEMVSGQWIWNWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZF56</accession>
    <name>A0AA38ZF56_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Flavonoid 3'-monooxygenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_013923</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9687933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZF56"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20618">
        <property type="entry name" value="CYP71_clan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000038">
        <property type="entry name" value="Cytochrome P450 84A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47944:SF5">
        <property type="entry name" value="CYTOCHROME P450 71A1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47944">
        <property type="entry name" value="CYTOCHROME P450 98A9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8 9" id="KW-0349">Heme</keyword>
    <keyword evidence="6 8 9" id="KW-0408">Iron</keyword>
    <keyword evidence="4 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041438815" description="Flavonoid 3'-monooxygenase" evidence="10">
        <location>
            <begin position="26"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="8">
        <location>
            <position position="446"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9687933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="509" mass="57976" checksum="54BC5C9D4189A2AE" modified="2024-01-24" version="1">MEAPSCVVLALVCLAAAALLSKLFSFRPPHKQNLPPGPKPWRIIGNLNLIGHLPHLSLHKLSQKYGQIMQLQFGSFPVVVASSPEMAKQFLKTNDHLFASRPQTAAGKYTAYNHSNITWAPYGPYWRQGRKIYHTELFSWKRLESYEYIRVEETRASVSRLYALSGNPVMLKDHLSRVTLSVISRIVLGEKYFNESESGRSIVTVEEFQEMLDELFLLNGVLNIGDWIPWIAFLDLQGYVKRMKALRDKFDRFFDHVIEKHRARREAEDFVAKDMVDMLLRLADDPDLEVKLGTDGVKGLTLDLLAGGTDTSATTMEWAMSEILRQPSIAKKATEELDRVIGRDRWVEEKDIPQLPYIDAIVKETMRLHPVAVLLAPHVALHDCNVAGYDIRRGTQVLVNTWSIGRDPNIWDAPEEFRPERFLGKAIDVKGQNFELLPFGSGRRMCAGYRLGLKMIRSSLANMLHGFHWTLPGDMKTEELNMEEVFGLTTPRKVPLVAVMEPRLPSHLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZFE0</accession>
    <name>A0AA38ZFE0_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_016187</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZFE0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9687597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="47" mass="5273" checksum="BF32DC02C9451323" modified="2024-01-24" version="1">MLKGRSCLFSRALPTIDIEKLQVEDANGKCLPDIDAQIFLLILYAHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZGV1</accession>
    <name>A0AA38ZGV1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nuclear pore complex protein NUP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_014360</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZGV1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071763">
        <property type="term" value="P:nuclear membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33416">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33416:SF18">
        <property type="entry name" value="NUCLEOPORIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="442"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="548"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="442"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9688667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="785" mass="85278" checksum="E16E8070C6429B9E" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZIN8</accession>
    <name>A0AA38ZIN8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FPL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_014827</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9689337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZIN8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901096">
        <property type="term" value="P:regulation of autophagosome maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039272">
        <property type="entry name" value="CLEC16A/TT9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019155">
        <property type="entry name" value="CLEC16A/TT9_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21481">
        <property type="entry name" value="PROTEIN CLEC16A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21481:SF4">
        <property type="entry name" value="PROTEIN TRANSPARENT TESTA 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09758">
        <property type="entry name" value="FPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FPL" evidence="2">
        <location>
            <begin position="42"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="841"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="852"/>
            <end position="862"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9689337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="862" mass="97507" checksum="3D3991B4585814F0" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZJD4</accession>
    <name>A0AA38ZJD4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Alpha-farnesene synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_012680</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZJD4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010333">
        <property type="term" value="F:terpene synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016102">
        <property type="term" value="P:diterpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00684">
        <property type="entry name" value="Terpene_cyclase_plant_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.600.10:FF:000007">
        <property type="entry name" value="Isoprene synthase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.130:FF:000001">
        <property type="entry name" value="Isoprene synthase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.130">
        <property type="entry name" value="Terpene synthase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034741">
        <property type="entry name" value="Terpene_cyclase-like_1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044814">
        <property type="entry name" value="Terpene_cyclase_plant_C1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001906">
        <property type="entry name" value="Terpene_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036965">
        <property type="entry name" value="Terpene_synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050148">
        <property type="entry name" value="Terpene_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005630">
        <property type="entry name" value="Terpene_synthase_metal-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225:SF94">
        <property type="entry name" value="ALPHA-FARNESENE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225">
        <property type="entry name" value="OS04G0344100 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01397">
        <property type="entry name" value="Terpene_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03936">
        <property type="entry name" value="Terpene_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01019">
        <property type="entry name" value="Terpene_Cyclase_Like_1_C_Termi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Terpene synthase N-terminal" evidence="5">
        <location>
            <begin position="38"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="Terpene synthase metal-binding" evidence="6">
        <location>
            <begin position="268"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9690150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="566" mass="64586" checksum="0643D06EF4A65031" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZKM9</accession>
    <name>A0AA38ZKM9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Subtilisin-like protease SBT5.6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_013143</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the peptidase S8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZKM9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02120">
        <property type="entry name" value="PA_subtilisin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04852">
        <property type="entry name" value="Peptidases_S8_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000006">
        <property type="entry name" value="Subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000005">
        <property type="entry name" value="subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.80:FF:000002">
        <property type="entry name" value="Subtilisin-like protease SBT5.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034197">
        <property type="entry name" value="Peptidases_S8_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010259">
        <property type="entry name" value="S8pro/Inhibitor_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045051">
        <property type="entry name" value="SBT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041469">
        <property type="entry name" value="Subtilisin-like_FN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10795">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17766">
        <property type="entry name" value="fn3_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05922">
        <property type="entry name" value="Inhibitor_I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 8" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041299856" description="Subtilisin-like protease SBT5.6" evidence="10">
        <location>
            <begin position="20"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="domain" description="Inhibitor I9" evidence="13">
        <location>
            <begin position="23"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="11">
        <location>
            <begin position="140"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="12">
        <location>
            <begin position="398"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="domain" description="Subtilisin-like protease fibronectin type-III" evidence="14">
        <location>
            <begin position="674"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="206"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="214"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7 8">
        <location>
            <position position="149"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7 8">
        <location>
            <position position="222"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7 8">
        <location>
            <position position="567"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF05922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF17766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9690843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="778" mass="83963" checksum="16C9E663793E5D36" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZKT1</accession>
    <name>A0AA38ZKT1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GDSL esterase/lipase At5g45910-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_013024</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZKT1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22835:SF659">
        <property type="entry name" value="GDSL LIPASE_ACYLHYDROLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G00510)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22835">
        <property type="entry name" value="ZINC FINGER FYVE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041221301" description="GDSL esterase/lipase At5g45910-like" evidence="5">
        <location>
            <begin position="22"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9690650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="364" mass="40164" checksum="F3EA38E85D603D25" modified="2024-01-24" version="1">MRFSFLIPFLFILCRFSTVSTCDKRYESIFSFGDSLADTGNFLLSGALAFPVIRELPYGETFFRHATGRCSDGRLIVDFIAKAFGIPYLPPYLSLGKGKSFRHGVNFAVAGATALDPEFFYHQKLGRILWTNSSLSVQLGWFKKLKPSICTTKKDCDNFFRKSIFLVGEIGGNDYNYPFFVGGSIKQVQALVPLVVEAITKAASMLIEEGAVTLMVPGNFPIGCSAVYLTIFRSPNKADYDENNGCLKAFNAFAQYHNTHLKLALDKLGLKYPHAKIIYADYYNAAMPLFQAPRSFGFYNGALRACCGGGGPYNFNNSARCGHIGSRACNDPSSYANWDGIHLTEGAYKIIATFLISNSFSSPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZLN3</accession>
    <name>A0AA38ZLN3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Subtilisin-like protease SBT2.2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_013524</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9 10">Belongs to the peptidase S8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZLN3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02120">
        <property type="entry name" value="PA_subtilisin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04852">
        <property type="entry name" value="Peptidases_S8_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000006">
        <property type="entry name" value="Subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034197">
        <property type="entry name" value="Peptidases_S8_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010259">
        <property type="entry name" value="S8pro/Inhibitor_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045051">
        <property type="entry name" value="SBT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041469">
        <property type="entry name" value="Subtilisin-like_FN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10795">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17766">
        <property type="entry name" value="fn3_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05922">
        <property type="entry name" value="Inhibitor_I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 9 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 9 10" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9 10" id="KW-0720">Serine protease</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041383048" description="Subtilisin-like protease SBT2.2" evidence="11">
        <location>
            <begin position="25"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="domain" description="Inhibitor I9" evidence="13">
        <location>
            <begin position="33"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="12">
        <location>
            <begin position="171"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="domain" description="Subtilisin-like protease fibronectin type-III" evidence="14">
        <location>
            <begin position="738"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8 9">
        <location>
            <position position="180"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8 9">
        <location>
            <position position="255"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8 9">
        <location>
            <position position="619"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF05922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF17766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9691368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="842" mass="90321" checksum="B57E495412DB45E7" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZLN8</accession>
    <name>A0AA38ZLN8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_013437</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZLN8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099402">
        <property type="term" value="P:plant organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000158">
        <property type="entry name" value="pentatricopeptide repeat-containing protein At2g33680"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000348">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF537">
        <property type="entry name" value="PENTACOTRIPEPTIDE-REPEAT REGION OF PRORP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="29"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="191"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="292"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9691262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="474" mass="52606" checksum="A8621F71EFDFA53A" modified="2024-01-24" version="1">MLKACWRMGSLSKGKEVHAHVTKTGLDSDVYVGNALLHLYGSTGQVTDARRLFDGMPHRDLASWNTLLGAYNDNAVEVLVLFKRMMYEGIGGDHISMVIVFSACGKIGGTEFGKEVHGYVIKVGIRPALSLSNALLWVYTRCGEMDAAQSLFVEMAAMRDVVSHTILFNGYVDMGSIDLARGIFDQMSVKDLVSWNSMIHAYVKAKHPKKALELFRKMENEMVEPDETTMVSVLAACASLADLQNGRLVHRFILQNNLRQDLFVGTALIDMYAKCGSLEEAMVTFYKMDSRDVFTWTTAIEGLANHGHGDKALSLFTKMEKQGIKPNQATFVSILMACSRSGLVKEGCLLFKRMVEAYQIQPKIEHLGCLLDILSRAGLLHQAEEFIKLMPPEEKIIANKTLLSACMNHLEYDLGEKIANGLTELSSQSHATHILLSNFYALAGQWAEVAKMRRAVKETDIRKVPGISSVDIKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZM44</accession>
    <name>A0AA38ZM44_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Lysine-specific demethylase JMJ25-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_013701</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the JARID1 histone demethylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZM44"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032454">
        <property type="term" value="F:histone H3K9 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02208">
        <property type="entry name" value="cupin_RmlC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045109">
        <property type="entry name" value="LSDs-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018866">
        <property type="entry name" value="Znf-4CXXC_R1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12549">
        <property type="entry name" value="JMJC DOMAIN-CONTAINING HISTONE DEMETHYLATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12549:SF11">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE JMJ25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10497">
        <property type="entry name" value="zf-4CXXC_R1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="133"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="JmjC" evidence="10">
        <location>
            <begin position="580"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="321"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="656"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="880"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="7"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="22"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="74"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="322"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="681"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="695"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="880"/>
            <end position="891"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9691590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="900" mass="102225" checksum="AAF9ED57637624CE" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZP38</accession>
    <name>A0AA38ZP38_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ARID domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_011104</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9691828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZP38"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16100">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15615">
        <property type="entry name" value="PHD_ARID4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.60">
        <property type="entry name" value="ARID DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042293">
        <property type="entry name" value="ARID4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001606">
        <property type="entry name" value="ARID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036431">
        <property type="entry name" value="ARID_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46694">
        <property type="entry name" value="AT-RICH INTERACTIVE DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46694:SF1">
        <property type="entry name" value="AT-RICH INTERACTIVE DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01388">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01014">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46774">
        <property type="entry name" value="ARID-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51011">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ARID" evidence="5">
        <location>
            <begin position="596"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="442"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9691828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="774" mass="85149" checksum="71D810872DD14EF7" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZPR7</accession>
    <name>A0AA38ZPR7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Molybdate transporter 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_011535</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZPR7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015098">
        <property type="term" value="F:molybdate ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031563">
        <property type="entry name" value="MOT1/MOT2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31970:SF9">
        <property type="entry name" value="MOLYBDATE TRANSPORTER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16983">
        <property type="entry name" value="MFS_MOT1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="172"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="227"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="354"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="379"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="409"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9692532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="469" mass="50255" checksum="EAED68B9FD859583" modified="2024-01-24" version="1">MANDTPSTTTTPLLRPSRRWFSSLRLKTTLSAELGGSVGDLGTYIPIVLALTLVNHLDLSTTLIFTALYNISTGFLFGIPMPVQPMKSIAAVAISDPLLTLPQIAAAGLSTAATLFILGATGLMSLLYRFIPLPVVRGVQLSQGLAFAFSAIKYIRNNQDFSAGKSGPPRNWLGLDGIVLALFAILFLVLVTGSGDFGDEAIPLGVHEEENNSVNRSRRRVRRRLRIMSSIPAALIVFILGLVLCFIRDPSIVKDLRFGPSRIHLLRITWEDWKIGFVRGAIPQIPLSILNSVIAVCKLSADLFPDREVSVTSVSVSVGVMNLVGCWFGAMPVCHGAGGLAGQYRFGGRSGASVVFLGLGKLLIGLVFGNSFVRILGQFPIGILGVLLLFAGIELAMASRDMNTKEESFVMLVCAAVSMTGSSAALGFGCGILLYGLLKLRQMECSCFGNFNFDPKRPVAVDDETSVNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZQL6</accession>
    <name>A0AA38ZQL6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Peptide deformylase</fullName>
            <ecNumber evidence="2 7">3.5.1.88</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_011612</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">Removes the formyl group from the N-terminal Met of newly synthesized proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>N-terminal N-formyl-L-methionyl-[peptide] + H2O = N-terminal L-methionyl-[peptide] + formate</text>
            <dbReference type="Rhea" id="RHEA:24420"/>
            <dbReference type="Rhea" id="RHEA-COMP:10639"/>
            <dbReference type="Rhea" id="RHEA-COMP:10640"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:49298"/>
            <dbReference type="ChEBI" id="CHEBI:64731"/>
            <dbReference type="EC" id="3.5.1.88"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Plastid</location>
            <location evidence="7">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the polypeptide deformylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.88" evidence="2 7"/>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZQL6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042586">
        <property type="term" value="F:peptide deformylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00487">
        <property type="entry name" value="Pep_deformylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.45.10:FF:000003">
        <property type="entry name" value="Peptide deformylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.45.10">
        <property type="entry name" value="Peptide deformylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00163">
        <property type="entry name" value="Pep_deformylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023635">
        <property type="entry name" value="Peptide_deformylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036821">
        <property type="entry name" value="Peptide_deformylase_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00079">
        <property type="entry name" value="pept_deformyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001159">
        <property type="entry name" value="PRK00150.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10458">
        <property type="entry name" value="PEPTIDE DEFORMYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10458:SF2">
        <property type="entry name" value="PEPTIDE DEFORMYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01327">
        <property type="entry name" value="Pep_deformylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01576">
        <property type="entry name" value="PDEFORMYLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56420">
        <property type="entry name" value="Peptide deformylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0934">Plastid</keyword>
    <keyword evidence="5 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9692623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="277" mass="30794" checksum="69EAEB4EC897C2C8" modified="2024-01-24" version="1">MESIGRFSQRLIPTCLAENCLRNQITSPFRLIPSLRFHPPLIPTSKPGPSDPNRIFTTRITYRPSSSSSTLIASAGWILGLGDKKPTLPEIVKAGDPVLHESAQEVEPGEIGSDRIQKIIDDMIKVMRTAPGVGLAAPQIGIPLRIIVLEDTKEYISYDRKDVIKAQERRPFDLLVILNPKLKKKGNRTAFFFEGCLSIDGFRAVVERHLQVEVTGLSRDGKPIKVDASGWKARILQHECDHLDGTLYVDKMVPRTFRTVQNIDLPLAVGCPKLGAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZRI3</accession>
    <name>A0AA38ZRI3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Retrovirus-related Pol polyprotein from transposon 17.6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_009744</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9693916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZRI3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.370:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 17.6-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041373">
        <property type="entry name" value="RT_RNaseH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17917">
        <property type="entry name" value="RT_RNaseH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="7">
        <location>
            <begin position="66"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase RNase H-like" evidence="8">
        <location>
            <begin position="239"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase zinc-binding" evidence="9">
        <location>
            <begin position="441"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF17921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9693916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="494" mass="57346" checksum="677DE0EF5B1957C6" modified="2024-01-24" version="1">MEEEAKPIRQPQRRLNPHLQEVVRAEVPKLLQAGIIYPISDSPWVSPTQVVPKKSRITVVQNEKGEEIATRLTSGWRVCIDYRKLNAVTRKNHFPLPFIDQVLERVSGHPFYYFLDGYSGYFQIEIDVEDQEKTTFTCPFGTYAYRRMPFGLCNAPATFQRCMLSIFSDMVERIMEVFMDDITVYGGTFEECLVNLEAVLNRCIEKDLVLNWEKCHFMSFDQLKQFLTTAPIVRPPNWQLPFEVMCDASDFAIGAVLGQREEGKPYVIYYASKTLNEAQRNYTTTEKELLAVVFTLDKFRAYLVGSFIIVFTDHSALKYLLTKQDAKARLIRWILLLQEFDLQIRDKKGVENVVANHLSRLAIAHNSHVLPINDDFPEESLMLLEKVPWYAHIANYLVTGEVPSEWKAQDKRHFFAKIHAYYWEEPFLFKYCANQIIRKCVPEQEQQGILSHCHENACGGHFASQKTAMKVLQSGFTWPSLFKDSHIMCRSCER</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZRQ6</accession>
    <name>A0AA38ZRQ6_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_012065</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9693164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZRQ6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050317">
        <property type="entry name" value="Plant_Fungal_Acyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31642:SF266">
        <property type="entry name" value="HXXXD-TYPE ACYL-TRANSFERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31642">
        <property type="entry name" value="TRICHOTHECENE 3-O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9693164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="466" mass="51834" checksum="6CEC39C32ADD2052" modified="2024-01-24" version="1">MGNGGDFTVTVTRKDVVAAVMPILEYWLPLSNLDLLLPPLDVSVFLCYKKPHESVNGCENFTFGSMVRVLKEAMAQALVPYYAFGGEVWSNSLGEPELLCNNHGVDFLEAYADVKLKDLDLYNPDESIEGKLVPKKKHGVLSVQVTELKCGGVVVACTFDHRIADAYSTNMFLVSWAEIAQCKPLSVIPSFRRSLLNPRHPGSYDPSIDNMYIPYSALPPPRAQQRQADPLISRLFYVMAEQLILLQRLATSKGSSYRRTKLESLTAFLWKMVAKSAAVVGNSNKQICKMGIVVDGRQRLSIGDEDKEAAMASYFGNVLSIAFGEKTIHELKEKPLSWVADAVHDYLEGAATKEHFLGLIDWVEAHRPEPALTKIYCSSSSDEPAFVMSSGQRFPVSKVDFGWGRPAVGSYHFPWGGETGYVMPMPSPARDGDWVVYMHLLKGQIELIEKEAAHIFRPLASEYLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZVR8</accession>
    <name>A0AA38ZVR8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Insulin-degrading enzyme-like 1, peroxisomal</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_011015</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the peptidase M16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZVR8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000003">
        <property type="entry name" value="Insulin-degrading enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000028">
        <property type="entry name" value="Insulin-degrading enzyme-like 1 peroxisomal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000005">
        <property type="entry name" value="nardilysin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000012">
        <property type="entry name" value="Protease 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001431">
        <property type="entry name" value="Pept_M16_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050626">
        <property type="entry name" value="Peptidase_M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032632">
        <property type="entry name" value="Peptidase_M16_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054734">
        <property type="entry name" value="PqqF-like_C_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43690:SF18">
        <property type="entry name" value="INSULIN-DEGRADING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43690">
        <property type="entry name" value="NARDILYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16187">
        <property type="entry name" value="Peptidase_M16_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22456">
        <property type="entry name" value="PqqF-like_C_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00143">
        <property type="entry name" value="INSULINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="9">
        <location>
            <begin position="29"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="10">
        <location>
            <begin position="176"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 middle/third" evidence="11">
        <location>
            <begin position="335"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="domain" description="Coenzyme PQQ synthesis protein F-like C-terminal lobe" evidence="12">
        <location>
            <begin position="727"/>
            <end position="825"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF22456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9695825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="924" mass="106170" checksum="911AA001D7F4CC54" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZW62</accession>
    <name>A0AA38ZW62_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Heat stress transcription factor A-6b</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_008352</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZW62"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF322">
        <property type="entry name" value="HEAT STRESS TRANSCRIPTION FACTOR A-7A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="127"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="133"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9696050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="192" mass="21864" checksum="05C4DD1D2E48F011" modified="2024-01-24" version="1">MMELVKLRQQQQNTRITLQAMEQRLQGTEIKQQQMMNFLARAMQNPAFIQQLVQQKEKRKELEEAISKKRRRPIDQGPSGAGGTESSQGNGLRNPIKIEPLHFTEGYGFEVSELEALAFEMQGLGRPRKELEDHEELDSPEGEDKELDEGFWEELLNERFGCPSSLPGAEGEEEEDVNVLADRFGYLGSSPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZW78</accession>
    <name>A0AA38ZW78_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_008586</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZW78"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9696435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="105" mass="11826" checksum="2C88E13D35D81A1C" modified="2024-01-24" version="1">MMTQMIRLIFRTEFDPDCTSSGSAANKEATFAERTSKALSLSIDTSEPEFGDVPNDFMAMRPPYSWQTQLYQTSLSRDVTDVQMSLSYGLEANKEAEFPGCTRTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZWK7</accession>
    <name>A0AA38ZWK7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TORTIFOLIA1/SINE1-2 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_010911</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZWK7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033337">
        <property type="entry name" value="TORTIFOLIA1/SINE1-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057600">
        <property type="entry name" value="TORTIFOLIA1/SINE1-2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31355">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEIN TORTIFOLIA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31355:SF4">
        <property type="entry name" value="TOG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24714">
        <property type="entry name" value="TOR1L1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="576"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="domain" description="TORTIFOLIA1/SINE1-2 N-terminal" evidence="3">
        <location>
            <begin position="18"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="470"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="495"/>
            <end position="506"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9695669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="606" mass="66814" checksum="4C5F49FBEB1229B3" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZYJ0</accession>
    <name>A0AA38ZYJ0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">YTH domain-containing family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_007004</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, and regulates mRNA stability. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in mRNA stability and processing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the YTHDF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9697671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZYJ0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990247">
        <property type="term" value="F:N6-methyladenosine-containing RNA reader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061157">
        <property type="term" value="P:mRNA destabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21134">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.590.10:FF:000001">
        <property type="entry name" value="YTH domain family 1, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.590.10">
        <property type="entry name" value="ph1033 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007275">
        <property type="entry name" value="YTH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045168">
        <property type="entry name" value="YTH_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12357:SF99">
        <property type="entry name" value="YTH DOMAIN-CONTAINING PROTEIN ECT2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12357">
        <property type="entry name" value="YTH YT521-B HOMOLOGY DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04146">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50882">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="YTH" evidence="6">
        <location>
            <begin position="451"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="22"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="140"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="198"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="390"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="162"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="226"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="413"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="424"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU369095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9697671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="705" mass="76903" checksum="0A0908E9B09B1046" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A3G6</accession>
    <name>A0AA39A3G6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Leucine-rich repeat receptor-like serine/threonine-protein kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_005883</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A3G6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000400">
        <property type="entry name" value="Nuclear pore complex protein NUP107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.430">
        <property type="entry name" value="Galactose-binding lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024788">
        <property type="entry name" value="Malectin-like_Carb-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45631:SF3">
        <property type="entry name" value="OS05G0393100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45631">
        <property type="entry name" value="OS07G0107800 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12819">
        <property type="entry name" value="Malectin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Malectin-like" evidence="9">
        <location>
            <begin position="63"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="8">
        <location>
            <begin position="392"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9700281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="549" mass="59929" checksum="406A09AD0758AE59" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A3M8</accession>
    <name>A0AA39A3M8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein DMP2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_005810</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the plant DMP1 protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A3M8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010256">
        <property type="term" value="P:endomembrane system organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007770">
        <property type="entry name" value="DMP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31621:SF66">
        <property type="entry name" value="PROTEIN DMP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31621">
        <property type="entry name" value="PROTEIN DMP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05078">
        <property type="entry name" value="DUF679"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="120"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9700159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="192" mass="20646" checksum="56F6E1BFAA5F050A" modified="2024-01-24" version="1">MGGTSKSSGSGKSTQPSITDRTFTGVGNLIKLLPTGTVFLFQFLNPVLTNNGHCHTINKYLTGILLGVCGFSCFFSSFTDSFFGSDGMTHYGVATKNGLWPSSASESVNLSAYKLRVEDFVHAFLSLTVFAVVALLDSNTVDCFYPSFESTEKLLLMVLPPVIGAISSTVFMVFPNKRHGIGYPASKTTHES</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A4J2</accession>
    <name>A0AA39A4J2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Trichome birefringence-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_005547</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PC-esterase family. TBL subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9699734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A4J2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016413">
        <property type="term" value="F:O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029962">
        <property type="entry name" value="TBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026057">
        <property type="entry name" value="TBL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025846">
        <property type="entry name" value="TBL_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285:SF241">
        <property type="entry name" value="PROTEIN TRICHOME BIREFRINGENCE-LIKE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285">
        <property type="entry name" value="PROTEIN TRICHOME BIREFRINGENCE-LIKE 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13839">
        <property type="entry name" value="PC-Esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14416">
        <property type="entry name" value="PMR5N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Trichome birefringence-like N-terminal" evidence="9">
        <location>
            <begin position="116"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="Trichome birefringence-like C-terminal" evidence="8">
        <location>
            <begin position="169"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="75"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="75"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="98"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF14416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9699734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="465" mass="53364" checksum="9CB7EE14225E7291" modified="2024-01-24" version="1">MASLKNLFSTLLHAPRTLSFPLSPPRTHFFVGLLRILSLFLFFSATFADTSPAVAAETLVSRLLSTSNRASPTTSCAHLVSNSHGTRTEGTSLSTVKETQKSPRNSESDAVGDLTSCDIFDGNWVLDDPDPVYPPRSCPYIDDAFNCLKNGRPDSDYLRFRWKPHGCQIPRFDGRKMLKMLRGKRLVFVGDSLNRNMWESLVCALRESLVDKSRVFEVSGKREFRTQGFYSFRFLDYKCSIDFVRSPFLVQETISRTLRATLRLDVIQDSSSKYRDADIIVFNTGHWWTHQKTYRGQNYFREGKHVYKRLEVTEAYTKALRTWAQWVDANIDSNRTRVFFRGFSASHFRGGQWNSGGNCDGETRPITNETQLAPYPWMMRTLESVLAEMITPVLYLNITKMTDYRKDGHPSIFRQPEASRSNEMVQDCSHWCLPGVPDSWNELLYATLLTSGYDSRSNRWISVKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A622</accession>
    <name>A0AA39A622_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_006745</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A622"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12347">
        <property type="entry name" value="RRM_PPIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000557">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034168">
        <property type="entry name" value="PPIE_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48037">
        <property type="entry name" value="ATPASE E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48037:SF1">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="8"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="126"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="133"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="155"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9701521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="173" mass="19559" checksum="600EF797ED68F404" modified="2024-01-24" version="1">MNQPVQKNTLYVGGLAEEVNESILHSAFIPFGDIKDVKTPLDQATQKHRSFGFVTFLEKEDAAAAMDNMDGAELYGRVLTVNYALPERIKGGEQGWAAQPIWADADTWFERQQQEEEMQRIQAENRAAMQAAEELHRKKMAQEREGEKEEEVESKDDPMARAEAEVLNQSNSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A6X6</accession>
    <name>A0AA39A6X6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">NB-ARC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_006800</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A6X6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF955">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0611">Plant defense</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NB-ARC" evidence="4">
        <location>
            <begin position="353"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="5">
        <location>
            <begin position="584"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="6">
        <location>
            <begin position="745"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="321"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="334"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9701577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1056" mass="122368" checksum="B7627ADCD8172549" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A737</accession>
    <name>A0AA39A737_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_006415</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the AP2/ERF transcription factor family. ERF subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A737"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009873">
        <property type="term" value="P:ethylene-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000001">
        <property type="entry name" value="Ethylene-responsive transcription factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051758">
        <property type="entry name" value="ERF/AP2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31657">
        <property type="entry name" value="ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR ERF061"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31657:SF40">
        <property type="entry name" value="ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR ERF062"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00367">
        <property type="entry name" value="ETHRSPELEMNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0936">Ethylene signaling pathway</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="10">
        <location>
            <begin position="227"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9701054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="404" mass="44817" checksum="6D71445948D892AF" modified="2024-01-24" version="1">MEDQFPKMQTFLRKELPSYFQGPMGSRFFGDATIWGGLAETASCNHVTGSNVKLSSSSPDSLFSSSESTSSVDEASASNLVKNMPEFTREDPLQKQHGVPFFSGSSSVNLSGASSTSDVKQRFIPEPSTVVIDPSIQIANSLGRNEKCEPMSSCPLFPTPQLSQSQHLPGFEWLKINQSLANHPSKGLSDYWLSTTKTQPMKYTGRRMLNHQQKTSLSSSASSPGKLFRGVRQRHWGKWVAEIRLPRNRTRVWLGTFDTAEEAAFAYDTAAYILRGDYAHLNFPELKHRLKANSTNGTTAALLEAKLQAISKGISAHKEPIAPPPPPSPKQHYLPHDNSKVAKAVSQNPTRKEWQLELEYKIAHEIAENKKIQEVVLEMDGVQLSRMPSLDMDAIWDAILVSDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A7C3</accession>
    <name>A0AA39A7C3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_004182</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A7C3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013666">
        <property type="entry name" value="PH_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040269">
        <property type="entry name" value="VAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008546">
        <property type="entry name" value="VAN3-bd-like_auxin_canal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31351">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31351:SF2">
        <property type="entry name" value="PHOSPHOINOSITIDE BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05703">
        <property type="entry name" value="Auxin_canalis"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08458">
        <property type="entry name" value="PH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="1">
        <location>
            <begin position="311"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9702318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="424" mass="46081" checksum="995BE500B9A455D8" modified="2024-01-24" version="1">MKSELAWKVSVGSCGGFKCPSRRLENIEEDGPASWLPETCAPPETPIESMEFLARSWSLSAMELAKALAADNLDKYSAFCSAGNEAEIHVASSVAPEEAIIQQLPGGGSPPISPRDSDDMKELFLLHQALNPDFLSNQQLLRNGLYKSIMRGKTFGRWLKDQKERKKQEIRTHNAQLHAAVSVAGVAAAVAALAASNAASPEVLATQHKKPSKTSAAIASAAALVASHCIEIAEDMGADHDHILMAVNSAVNARTNGDIMTLTAGAATALRGAATLRARLQKGCGTATLVLSEEQIEEGRESNILIALNFVSKGGELLKRTRKGALHWKQVSFNINSCWQVAVKMKSKHMAGTFTKKKKCVVSGVYCDIPAWPGREREESSEQRAYFGIKTDDRIIEFECRNNGDKQMWAEGIQYMLHCRASMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A7S2</accession>
    <name>A0AA39A7S2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_004025</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A7S2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47941">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12854">
        <property type="entry name" value="PPR_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="162"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="198"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="230"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="266"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="301"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="336"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="371"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="406"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="441"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="477"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="570"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="576"/>
            <end position="593"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9702080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="593" mass="66058" checksum="3665FDF0206B5785" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A7Y9</accession>
    <name>A0AA39A7Y9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Alpha/beta hydrolase fold-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_004351</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDXG' lipolytic enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A7Y9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050466">
        <property type="entry name" value="Carboxylest/Gibb_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033140">
        <property type="entry name" value="Lipase_GDXG_put_SER_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23024">
        <property type="entry name" value="ARYLACETAMIDE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23024:SF635">
        <property type="entry name" value="OS07G0162700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01174">
        <property type="entry name" value="LIPASE_GDXG_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="4">
        <location>
            <begin position="70"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="18"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="27"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9702572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="309" mass="34364" checksum="09B04BED90DDBC63" modified="2024-01-24" version="1">MSIIAEEPGFIQIFSDGSVKRPERETSPASEDSSSTGYKSKDVVIDSTKPISGRIFVPDTPASSSLLPVLVYFHGGGFCIGTTTWLGYHTFLGDFAVAAQSIVLSVDYRLAPEHRLPTAYDDCYCSLEWLSKQVCSEPWLQRADLSRVFLSGDSAGGNIAHNIAIRAIQKGCDEVKIKGVLPIHPYFGSEERIDKEKASESAKGVGLTDLLWKLSLPEGSNRDYFGCNFEKAELSRDEWGRFPAVVVYVAGLDFFKERGVMYAGFLEKRGVEVKLVEAEGEQHVYHMFHPKSEATRLLQKQMSEFIHSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A823</accession>
    <name>A0AA39A823_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_004113</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A823"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05121">
        <property type="entry name" value="ABC1_ADCK3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="entry name" value="DD-peptidase/beta-lactamase superfamily"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004147">
        <property type="entry name" value="ABC1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001466">
        <property type="entry name" value="Beta-lactam-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051130">
        <property type="entry name" value="Mito_struct-func_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43173">
        <property type="entry name" value="ABC1 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43173:SF3">
        <property type="entry name" value="ABC1 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03109">
        <property type="entry name" value="ABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00144">
        <property type="entry name" value="Beta-lactamase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="beta-lactamase/transpeptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC1 atypical kinase-like" evidence="3">
        <location>
            <begin position="89"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-lactamase-related" evidence="2">
        <location>
            <begin position="479"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="752"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="797"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="831"/>
            <end position="843"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9702215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="964" mass="107398" checksum="1813013785DE7997" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A8F1</accession>
    <name>A0AA39A8F1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">TFIID subunit TAF5 NTD2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_004384</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat TAF5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A8F1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08044">
        <property type="entry name" value="TAF5_NTD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.500">
        <property type="entry name" value="TFIID subunit TAF5, NTD2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007582">
        <property type="entry name" value="TFIID_NTD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037264">
        <property type="entry name" value="TFIID_NTD2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879:SF1">
        <property type="entry name" value="CANNONBALL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04494">
        <property type="entry name" value="TFIID_NTD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160897">
        <property type="entry name" value="Taf5 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3 8" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="TFIID subunit TAF5 NTD2" evidence="10">
        <location>
            <begin position="66"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="351"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="421"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="463"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="505"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="547"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="589"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="247"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="34"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="252"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9702619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="676" mass="75150" checksum="8C9CEE297B0C3462" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A8L4</accession>
    <name>A0AA39A8L4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">4-coumarate--CoA ligase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_004605</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A8L4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12118">
        <property type="entry name" value="ttLC_FACS_AEE21_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000008">
        <property type="entry name" value="2,3-dihydroxybenzoate-AMP ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000003">
        <property type="entry name" value="Long-chain-fatty-acid--CoA ligase FadD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006020">
        <property type="entry name" value="PRK08162.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43859">
        <property type="entry name" value="ACYL-ACTIVATING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43859:SF57">
        <property type="entry name" value="ACYL-ACTIVATING ENZYME 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="3">
        <location>
            <begin position="21"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="4">
        <location>
            <begin position="452"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9702932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="550" mass="60512" checksum="486C8949DB3C39A6" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A9B9</accession>
    <name>A0AA39A9B9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">LOW protein: ammonium transporter 1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_004735</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A9B9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36802">
        <property type="entry name" value="OS02G0815400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36802:SF1">
        <property type="entry name" value="OS02G0815400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="280"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9703084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="311" mass="33519" checksum="C5E539F0E4B5D741" modified="2024-01-24" version="1">MASLLHLRHPHSVFNLSSASPTLLFHHSPHHIPLKPSHSTSITLKPLHFRPHTAPSALTDSDSPKSLSPNPETPQTLLQELADSFDLPSDYFAQLPRDLRLDLNDAAFDLSSGPVIDECGQELGETLLNLSRAWEQADTSSSSTLVSKLPSLEGSLTDNAKTAFGKRLVSAGRRFQSMGQYGQGELQRIAKAMTMTGKLLCASSVSAETNVQPKEETRMLKFGELQVSLTSDKANIGAAIGFVFGILSWQLSQGVQSIPESSLQYANDNALLLAKSLRGALLAICYSSTALSALATVGLILLARELKSEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A9U2</accession>
    <name>A0AA39A9U2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 19">Peroxidase</fullName>
            <ecNumber evidence="4 19">1.11.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PVL29_005066</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 19">
            <text>2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:56136"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:139520"/>
            <dbReference type="ChEBI" id="CHEBI:139521"/>
            <dbReference type="EC" id="1.11.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16 19">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="16 19">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16 19">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="16 19">Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="19">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peroxidase family. Ascorbate peroxidase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.7" evidence="4 19"/>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A9U2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140825">
        <property type="term" value="F:lactoperoxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00693">
        <property type="entry name" value="secretory_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.420.10:FF:000001">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.520.10:FF:000001">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.420.10">
        <property type="entry name" value="Peroxidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000823">
        <property type="entry name" value="Peroxidase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019794">
        <property type="entry name" value="Peroxidases_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019793">
        <property type="entry name" value="Peroxidases_heam-ligand_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033905">
        <property type="entry name" value="Secretory_peroxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31388:SF3">
        <property type="entry name" value="PEROXIDASE 72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31388">
        <property type="entry name" value="PEROXIDASE 72-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00458">
        <property type="entry name" value="PEROXIDASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00461">
        <property type="entry name" value="PLPEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00435">
        <property type="entry name" value="PEROXIDASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00436">
        <property type="entry name" value="PEROXIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50873">
        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 16 19" id="KW-0106">Calcium</keyword>
    <keyword evidence="11 18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 19" id="KW-0349">Heme</keyword>
    <keyword evidence="13 19" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="10 16 19" id="KW-0408">Iron</keyword>
    <keyword evidence="7 16 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 19" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5 19" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="19" id="KW-0964">Secreted</keyword>
    <keyword evidence="19" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041485281" description="Peroxidase" evidence="19">
        <location>
            <begin position="23"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="Plant heme peroxidase family profile" evidence="20">
        <location>
            <begin position="29"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="70"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="16">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="17">
        <location>
            <position position="66"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="39"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="72"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="125"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="204"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU362060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAJ9703577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="331" mass="36396" checksum="24CDA90A6E2AA417" modified="2024-01-24" version="1">MARFMSLFLTLSLLGFVPLSLSHKTNGGYLYPQFYDHSCPKAQQIVKSVMAKAVAREVRMAASIMRLHFHDCFVKGCDGSILLDSSGSIISEKNSVPNRNSARGFDVVDEIKSAVEKECPHTVSCADILAIAARDSSVLTGGPSWEVPLGRRDSRGASLSGSNNNIPAPNNTFQTILTKFKLKGLNIVDLVALSGSHTIGDSRCTSFRQRLYNQSGNGRPDYSLDQSYAAKLRTRCPRSGGDQNLFFLDFVSPTKFDNSYFKNILASKGLLSSDQVLYTKNEASMDLVKQYAANNKLFFEQFAQSMIKMANISPLTGSRGEIRKNCRKVNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AA17</accession>
    <name>A0AA39AA17_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_004391</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AA17"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF78">
        <property type="entry name" value="OS12G0587133 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9702628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="373" mass="42588" checksum="9EA635D6DE9AD5DA" modified="2024-01-24" version="1">MRGCLSSVSFAILVNGNAKGWVKASRGVRQGDPLSPFLFTLVADVLSKMLLRAEERNALEGFKVGRNRTRVSHLQFADDTIFFSSAREEGMLTLKSVLLVFGHIFGLKVNLDKSNIYGINLGQDHLYRLVELLNCKVSGGPILYLGLPLGGNPKACGFWDPVIERISSRLDGWQKTYLSFRGRITLIQSCLTHMPCYFLSLFKIPTSMTARIERMQRDFLWSGVGEGKKDHLVSWDVVCIPKAKRGLSLGRISLRNSALLGKWLWRFPRKGSALWHQVILSIYGSHSNGWDANTFVRWSHRCPWKAIAQVSQEFSKFTRFVVGYGERIRFWEDLWWRDQPLGVRYPRLLRVVTDKNIPISSILGSTRPFSWNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AAE6</accession>
    <name>A0AA39AAE6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Glutamate receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PVL29_004537</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Glutamate-gated receptor that probably acts as a non-selective cation channel. May be involved in light-signal transduction and calcium homeostasis via the regulation of calcium influx into cells.</text>
    </comment>
    <comment type="function">
        <text evidence="15">Glutamate-gated receptor that probably acts as non-selective cation channel.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">May form heteromers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AAE6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015276">
        <property type="term" value="F:ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13686">
        <property type="entry name" value="GluR_Plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19990">
        <property type="entry name" value="PBP1_GABAb_receptor_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000037">
        <property type="entry name" value="Glutamate receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000103">
        <property type="entry name" value="Glutamate receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000169">
        <property type="entry name" value="Glutamate receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000758">
        <property type="entry name" value="Glutamate receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044440">
        <property type="entry name" value="GABAb_receptor_plant_PBP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015683">
        <property type="entry name" value="Ionotropic_Glu_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001320">
        <property type="entry name" value="Iontro_rcpt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017103">
        <property type="entry name" value="Iontropic_Glu_rcpt_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34836">
        <property type="entry name" value="OS06G0188250 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34836:SF1">
        <property type="entry name" value="OS09G0428600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00060">
        <property type="entry name" value="Lig_chan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037090">
        <property type="entry name" value="Iontro_Glu-like_rcpt_pln"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00079">
        <property type="entry name" value="PBPe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13 15" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 15" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="9 15 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 15" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 18" id="KW-0732">Signal</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 15" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041471035" description="Glutamate receptor" evidence="18">
        <location>
            <begin position="29"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="584"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="644"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="822"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor C-terminal" evidence="19">
        <location>
            <begin position="456"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="749"/>
            <end position="805"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR037090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR037090-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAJ9702838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="957" mass="107134" checksum="CA5141EEF24F4B77" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ACT4</accession>
    <name>A0AA39ACT4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transcription termination factor MTERF6, chloroplastic/mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_002981</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the mTERF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ACT4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.70.10:FF:000012">
        <property type="entry name" value="transcription termination factor MTERF6, chloroplastic/mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.70.10">
        <property type="entry name" value="Transcription termination factor 3, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003690">
        <property type="entry name" value="MTERF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038538">
        <property type="entry name" value="MTERF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13068">
        <property type="entry name" value="CGI-12 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13068:SF112">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02536">
        <property type="entry name" value="mTERF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00733">
        <property type="entry name" value="Mterf"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0806">Transcription termination</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9704697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="354" mass="40194" checksum="B9D986685ACB4CCD" modified="2024-01-24" version="1">MASFLTSLKPFPLSLPDFLGKGDMEISSSQNGSIMWFFRDKGFDDKSIHEMFKKCKRLEGVHRDRASENWAYLRTIGIQDRKIPSIVTKCPKILALGLNEKIVPMVQCLATLGTKPGEVASAIAKFPHILSHSVEEKLCPLLAFFQALGVPEQQLGKVILVNPRLISYSIESKLTQIVDFLASLGLTREGMIGKVLLKHPFIMGYSVDKRLRPTSEFLKLVGLTGQDLQKVAMNFPEVFCRDANKILSPNVAYLKRRGFEDGQIAALVTGYPPILIKSIKNSLEPRIRFLVEVMRRDMNDVVNYPDFFRCGLKKTLELRQKLLEQRKIECSLSEMLDCNQKKFLLKFNLIERPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ADI4</accession>
    <name>A0AA39ADI4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ubiquinone biosynthesis monooxygenase COQ6, mitochondrial</fullName>
            <ecNumber evidence="11">1.14.15.45</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">2-methoxy-6-polyprenolphenol 4-hydroxylase</fullName>
            <ecNumber evidence="11">1.14.15.46</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">COQ6</name>
        <name evidence="13" type="ORF">PVL29_003602</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">FAD-dependent monooxygenase required for two non-consecutive steps during ubiquinone biosynthesis. Required for the C5-ring hydroxylation during ubiquinone biosynthesis by catalyzing the hydroxylation of 4-hydroxy-3-(all-trans-polyprenyl)benzoic acid to 3,4-dihydroxy-5-(all-trans-polyprenyl)benzoic acid. Also acts downstream of coq4, for the C1-hydroxylation during ubiquinone biosynthesis by catalyzing the hydroxylation of 2-methoxy-6-(all-trans-polyprenyl)phenol to 2-methoxy-6-(all-trans-polyprenyl)benzene-1,4-diol. The electrons required for the hydroxylation reaction are funneled indirectly to coq6 from NADPH via a ferredoxin/ferredoxin reductase system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 2-methoxy-6-(all-trans-polyprenyl)phenol + 2 reduced [2Fe-2S]-[ferredoxin] + O2 + 2 H(+) = a 2-methoxy-6-(all-trans-polyprenyl)benzene-1,4-diol + 2 oxidized [2Fe-2S]-[ferredoxin] + H2O</text>
            <dbReference type="Rhea" id="RHEA:81183"/>
            <dbReference type="Rhea" id="RHEA-COMP:9551"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:10858"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:62731"/>
            <dbReference type="ChEBI" id="CHEBI:84166"/>
            <dbReference type="EC" id="1.14.15.46"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 4-hydroxy-3-(all-trans-polyprenyl)benzoate + 2 reduced [2Fe-2S]-[ferredoxin] + O2 + 2 H(+) = a 3,4-dihydroxy-5-(all-trans-polyprenyl)benzoate + 2 oxidized [2Fe-2S]-[ferredoxin] + H2O</text>
            <dbReference type="Rhea" id="RHEA:81195"/>
            <dbReference type="Rhea" id="RHEA-COMP:9514"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:10930"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:64694"/>
            <dbReference type="ChEBI" id="CHEBI:78396"/>
            <dbReference type="EC" id="1.14.15.45"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="11">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of a multi-subunit COQ enzyme complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Mitochondrion inner membrane</location>
            <topology evidence="11">Peripheral membrane protein</topology>
            <orientation evidence="11">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the UbiH/COQ6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.15.45" evidence="11"/>
    <dbReference type="EC" id="1.14.15.46" evidence="11"/>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ADI4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031314">
        <property type="term" value="C:extrinsic component of mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120538">
        <property type="term" value="F:2-methoxy-6-polyprenolphenol 4-hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106364">
        <property type="term" value="F:4-hydroxy-3-all-trans-polyprenylbenzoate oxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016712">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016120">
        <property type="term" value="P:carotene biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016123">
        <property type="term" value="P:xanthophyll biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000021">
        <property type="entry name" value="Ubiquinone biosynthesis monooxygenase COQ6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.9.10:FF:000111">
        <property type="entry name" value="Ubiquinone biosynthesis monooxygenase COQ6, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000205">
        <property type="entry name" value="Ubiquinone biosynthesis monooxygenase COQ6, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03193">
        <property type="entry name" value="COQ6_monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018168">
        <property type="entry name" value="Ubi_Hdrlase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010971">
        <property type="entry name" value="UbiH/COQ6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051205">
        <property type="entry name" value="UbiH/COQ6_monooxygenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000689">
        <property type="entry name" value="UbQ_mOase_COQ6"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01989">
        <property type="entry name" value="COQ6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01988">
        <property type="entry name" value="Ubi-OHases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43876">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS MONOOXYGENASE COQ6, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43876:SF7">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS MONOOXYGENASE COQ6, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01304">
        <property type="entry name" value="UBIH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0274">FAD</keyword>
    <keyword evidence="3 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 11" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="8 11" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="domain" description="FAD-binding" evidence="12">
        <location>
            <begin position="90"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding" evidence="12">
        <location>
            <begin position="408"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9705615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="542" mass="59894" checksum="8F36EED37DA41186" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AEK3</accession>
    <name>A0AA39AEK3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Wall-associated receptor kinase galacturonan-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_003575</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AEK3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013695">
        <property type="entry name" value="WAK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025287">
        <property type="entry name" value="WAK_GUB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33491">
        <property type="entry name" value="OSJNBA0016N04.9 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13947">
        <property type="entry name" value="GUB_WAK_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08488">
        <property type="entry name" value="WAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041355201" description="Wall-associated receptor kinase galacturonan-binding domain-containing protein" evidence="7">
        <location>
            <begin position="43"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="Wall-associated receptor kinase galacturonan-binding" evidence="9">
        <location>
            <begin position="50"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="Wall-associated receptor kinase" evidence="8">
        <location>
            <begin position="184"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9705575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="317" mass="34941" checksum="5658FA5DBBEB4D25" modified="2024-01-24" version="1">MLFLRILYFAAYESQFNNSRDENCQWLLQISLLLWLSIGAAAATQGMPGCQERCGDVDIPYPFGIGFACYFDEWFEVTCNKSTDLPKPFLNIINMEVLSISLDRGTIQVNNPVLFDCSGEPSNVTGAPNFTVRWEGGPFSFSENYTRFTAVGCGVLAYIEQSDAIAGGCMSSCKPNETAEKKGSCYGLGCCQTEVPPALQSFTAYLDSFHSHYHGRLPCKSAFMVDQEWFISNGQDPDKVTGMDYVPAVLDWRIYNAKCNTSTSTRDTSTITVNCGNNTVCSSTNQDSSVTCDCLHGYEGNPYLPQGRQGTQSTCYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AEM2</accession>
    <name>A0AA39AEM2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_001608</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AEM2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000778">
        <property type="entry name" value="Kinase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010632">
        <property type="entry name" value="DUF1221"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050167">
        <property type="entry name" value="Ser_Thr_protein_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23257:SF969">
        <property type="entry name" value="INTEGRIN-LINKED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23257">
        <property type="entry name" value="SERINE-THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06760">
        <property type="entry name" value="DUF1221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="228"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="562"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="604"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="571"/>
            <end position="581"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9706127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="668" mass="75962" checksum="C935EEE70E8D67AA" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AFD6</accession>
    <name>A0AA39AFD6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Scarecrow-like protein 28</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_001859</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the GRAS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AFD6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005202">
        <property type="entry name" value="TF_GRAS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31636">
        <property type="entry name" value="OSJNBA0084A10.13 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03514">
        <property type="entry name" value="GRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50985">
        <property type="entry name" value="GRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="121"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="263"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="VHIID" evidence="3">
        <location>
            <begin position="374"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Leucine repeat II (LRII)" evidence="3">
        <location>
            <begin position="449"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="PFYRE" evidence="3">
        <location>
            <begin position="490"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="SAW" evidence="3">
        <location>
            <begin position="584"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="VHIID" evidence="3">
        <location>
            <begin position="405"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="272"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9706596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="676" mass="75244" checksum="B6418842EC36E186" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AIF4</accession>
    <name>A0AA39AIF4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Coiled-coil domain-containing protein 130</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_000255</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CWC16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AIF4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005684">
        <property type="term" value="C:U2-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007590">
        <property type="entry name" value="Saf4/Yju2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12111">
        <property type="entry name" value="SPLICING FACTOR YJU2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12111:SF2">
        <property type="entry name" value="SPLICING FACTOR YJU2B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04502">
        <property type="entry name" value="Saf4_Yju2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="279"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="291"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9708106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="313" mass="35251" checksum="CD4BC42A76FF15C2" modified="2024-01-24" version="1">MSSLAAARADNFYYPPEWTPNQGSLNKFHGQHALRERARKLDQGILIIRFEMPFNIWCGGCNSMIAKGVRFNAEKKQVGNYYSTKIWSFTMKSACCKHEIVIQTDPKNCEYVIISGAQRKTEEFDIEDAETFALPADEERGKLADPFYRLEHEGEDLQKKKEAEPVLVRLQRVSDARHSDDYSLNKALRAQLRNQKKRVAEEELVSKKLGLGIRLLPATEEDAATAARMKFSSKFERNRKEKRALINAASIFPGSSGSSMSDKKRLELGSKRRKIRAGTATELLTGGFKPSSWLKSSVSSSQSRGVSAASRHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AR86</accession>
    <name>A0AA39AR86_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_001433</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AR86"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000024">
        <property type="entry name" value="Somatic embryogenesis receptor kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47988">
        <property type="entry name" value="SOMATIC EMBRYOGENESIS RECEPTOR KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041372202" description="Leucine-rich repeat-containing N-terminal plant-type domain-containing protein" evidence="4">
        <location>
            <begin position="22"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="5">
        <location>
            <begin position="27"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="6">
        <location>
            <begin position="74"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9709948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="219" mass="24496" checksum="A7612B2DAF2F71AB" modified="2024-01-24" version="1">MAIWAQYHHLLALFLLTWASAVPTNANMEGDALYALRRAVKDPEHVLQSWDPTLVDPCTWFHVTCDSDNRVTRLDLGNAKLSGNLVPELGKLERLQYLELYMNNLVGPIPEQLGGLKNLVSLDLFHNNLTGSIPPSLSKLSNLRFLRLNRNRLSGTIPRELTKLGSLKILDVSNNDLCGTIPTTGSFSKFSEESFMNNPRLEGPELMGFVRYDTRGGCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D798</accession>
    <name>A0AA39D798_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_023097</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HMGB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D798"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22005">
        <property type="entry name" value="HMG-box_AtHMGB1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031061">
        <property type="entry name" value="HMGB_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46261:SF22">
        <property type="entry name" value="HIGH MOBILITY GROUP B PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46261">
        <property type="entry name" value="HIGH MOBILITY GROUP B PROTEIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="7">
        <location>
            <begin position="44"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="5">
        <location>
            <begin position="44"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="149"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="171"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9673299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="187" mass="21277" checksum="8778CF18EEF31E1D" modified="2024-01-24" version="1">MKGGRSKSDTKKADAKLSVKKGAAATKAGKKTKKEKPVKDPNKPKRPASAFFVFMEEFRKSYKEKHPSNKSVSVVGKAGGDKWKSMSDAEKAPYVAKAEKRKVEYEKNMKAYNKKQTFWDFYHCPLFWSVFSLQNSLERLLMTSHFDGNQAEGTKVVEEEDESEKSLSEVNDQEEDEDGSEEEEDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D7J9</accession>
    <name>A0AA39D7J9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PVL29_025675</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D7J9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000015">
        <property type="entry name" value="Calcium-dependent protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000056">
        <property type="entry name" value="calcium-dependent protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000004">
        <property type="entry name" value="Calcium-dependent protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0519">Myristate</keyword>
    <keyword evidence="9 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="88"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="20">
        <location>
            <begin position="389"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="20">
        <location>
            <begin position="425"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="20">
        <location>
            <begin position="461"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="20">
        <location>
            <begin position="497"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="34"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="58"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAJ9672127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="536" mass="59730" checksum="FC3012C4A91A2374" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D7V5</accession>
    <name>A0AA39D7V5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Thylakoid lumenal 29 kDa protein, chloroplastic</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 10">p29</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_023445</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast thylakoid lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 11">Belongs to the peroxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D7V5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009543">
        <property type="term" value="C:chloroplast thylakoid lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000302">
        <property type="term" value="P:response to reactive oxygen species"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00314">
        <property type="entry name" value="plant_peroxidase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.520.10:FF:000013">
        <property type="entry name" value="thylakoid lumenal 29 kDa protein, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1620:FF:000001">
        <property type="entry name" value="Thylakoid lumenal 29 kDa protein, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044831">
        <property type="entry name" value="Ccp1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002207">
        <property type="entry name" value="Peroxidase_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31356:SF34">
        <property type="entry name" value="THYLAKOID LUMENAL 29 KDA PROTEIN, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31356">
        <property type="entry name" value="THYLAKOID LUMENAL 29 KDA PROTEIN, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00459">
        <property type="entry name" value="ASPEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0934">Plastid</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0793">Thylakoid</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Plant heme peroxidase family profile" evidence="12">
        <location>
            <begin position="94"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00060884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00071440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00080823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00081383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9673899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="344" mass="37586" checksum="0A05564647213123" modified="2024-01-24" version="1">MGVSFLSTAPSLIPMAPQPSSTPRHSLHAGRIRCNRIECNGTDEDGFRRRDVLKCVGATLGMELLASSGPFVEVAAAADLIQLRQRSEFRSNVKGTLFTAIKGNPDVIPFLLTLALNDAMTYDKATKSGGPNGSIRFSSEISRPENKGLSAALNLLEESKKEIDSYSKGGPISFADLIQYAAQSAVKSTFIASAVRKCGGNEEKGNLLYTAYGSNGQWGLFDKQFGRADTQEPDPEGRVPQWEKASVQEMKDKFKAVGFGPRQLAVMSAFLGPDQLASEAFLATDPDVAPWVQKYQRSRETVSQTDYEVDLITTLTKLSCLGQQINYEAYTYPVQKVDFSKLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D811</accession>
    <name>A0AA39D811_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_023558</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D811"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33168">
        <property type="entry name" value="STRESS INDUCED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9674079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="102" mass="11792" checksum="BCD5120A65E3FFA6" modified="2024-01-24" version="1">MDPKKPGDEEEGGLLSCWGNLKLKFPWNKGRRNTQTRHRRVGWKIMEAFKPKRPKPFSSFRYDPLSYAQNFDDGCWDEDCEGSSYRGFSARFAVPSSKPLGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D9U7</accession>
    <name>A0AA39D9U7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Sugar phosphate transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_024445</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D9U7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004853">
        <property type="entry name" value="Sugar_P_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050186">
        <property type="entry name" value="TPT_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03151">
        <property type="entry name" value="TPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="43"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="78"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="133"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="156"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="186"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="219"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="250"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="279"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="Sugar phosphate transporter" evidence="7">
        <location>
            <begin position="23"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="306"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9675520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="334" mass="36611" checksum="0522D10433529AB7" modified="2024-01-24" version="1">MAPASKADKKAALDAAAWMFNVVTSVGIILVNKALMATYGFSFATTLTGLHFATTTLMTAILRWLGYIQPSHLPLPELLKFVLFANFSIVGMNVSLMWNSVGFYQIAKLSMIPVSCLLEVVLDKIRYSRDTKLSISVVLLGVAVCTVTDVSVNARGFIAAFIAVWSTALQQYYVHFLQRKYSLSSFNLLGHTAPAQAGSLLLLGPFLDYWLTNKRVDMYQFSTASLIFIILSCTIAVGTNLSQFICIGRFTAVSFQVLGHMKTILVLIMGFFFFGKEGLNLQVVLGMIIAVVGMMWYGNASSKPGGKERFHSLPTSKQQKHGGLSESSEHDGKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DA60</accession>
    <name>A0AA39DA60_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase</fullName>
            <ecNumber evidence="1">3.2.2.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_024726</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>NAD(+) + H2O = ADP-D-ribose + nicotinamide + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16301"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57967"/>
            <dbReference type="EC" id="3.2.2.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:16302"/>
        </physiologicalReaction>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.6" evidence="1"/>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DA60"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061809">
        <property type="term" value="F:NAD+ nucleosidase activity, cyclic ADP-ribose generating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045344">
        <property type="entry name" value="C-JID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058192">
        <property type="entry name" value="WHD_ROQ1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017:SF479">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN (TIR-NBS-LRR CLASS) FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20160">
        <property type="entry name" value="C-JID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23282">
        <property type="entry name" value="WHD_ROQ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="TIR" evidence="8">
        <location>
            <begin position="20"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9675895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1075" mass="121840" checksum="253ACBD3A6703B9C" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DB61</accession>
    <name>A0AA39DB61_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_022580</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DB61"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9677681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="53" mass="6029" checksum="68E2C4F4EB6F9DC3" modified="2024-01-24" version="1">MSMVEPNLEGFLNQKKQVKNHYFPLVGVLAAGIISFRQGNSHFQMLMRAHVDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DB87</accession>
    <name>A0AA39DB87_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PWWP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_022629</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DB87"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05162">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000313">
        <property type="entry name" value="PWWP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053063">
        <property type="entry name" value="PWWP_domain_containing_PDP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42851">
        <property type="entry name" value="ALDOLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42851:SF8">
        <property type="entry name" value="PWWP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00855">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00293">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50812">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PWWP" evidence="2">
        <location>
            <begin position="221"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="410"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="595"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="739"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="822"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="415"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="432"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="490"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="508"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="529"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9677756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1223" mass="131730" checksum="77D76381BF84A02C" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2025-04-02" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DBG4</accession>
    <name>A0AA39DBG4_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_022574</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00183">
        <property type="entry name" value="TFIIS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.10">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001025">
        <property type="entry name" value="BAH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043151">
        <property type="entry name" value="BAH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003617">
        <property type="entry name" value="TFIIS/CRSP70_N_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035441">
        <property type="entry name" value="TFIIS/LEDGF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017923">
        <property type="entry name" value="TFIIS_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46548">
        <property type="entry name" value="BAH AND TFIIS DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46548:SF1">
        <property type="entry name" value="BAH AND TFIIS DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01426">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08711">
        <property type="entry name" value="Med26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00439">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00509">
        <property type="entry name" value="TFS2N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47676">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51038">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51319">
        <property type="entry name" value="TFIIS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BAH" evidence="5">
        <location>
            <begin position="43"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="TFIIS N-terminal" evidence="6">
        <location>
            <begin position="327"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="183"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="421"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="480"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="635"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="795"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="901"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="969"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1120"/>
            <end position="1151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1289"/>
            <end position="1315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1558"/>
            <end position="1590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1625"/>
            <end position="1646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="23"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="198"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="230"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="480"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="527"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="557"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="591"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="659"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="671"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="689"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="724"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="824"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="906"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="916"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1136"/>
            <end position="1151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1291"/>
            <end position="1303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1625"/>
            <end position="1639"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9677664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1646" mass="176136" checksum="705CD62BCD567305" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DCR4</accession>
    <name>A0AA39DCR4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Flavin-containing monooxygenase</fullName>
            <ecNumber evidence="8">1.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_022473</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the conversion of methylthioalkyl glucosinolates of any chain length into methylsulfinylalkyl glucosinolates.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the FMO family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.-.-.-" evidence="8"/>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DCR4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004499">
        <property type="term" value="F:N,N-dimethylaniline monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000147">
        <property type="entry name" value="Flavin-containing monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000960">
        <property type="entry name" value="Flavin_mOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020946">
        <property type="entry name" value="Flavin_mOase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050346">
        <property type="entry name" value="FMO-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23023">
        <property type="entry name" value="DIMETHYLANILINE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00743">
        <property type="entry name" value="FMO-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000332">
        <property type="entry name" value="FMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00370">
        <property type="entry name" value="FMOXYGENASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0274">FAD</keyword>
    <keyword evidence="3 8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="6 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00058243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9677497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="448" mass="50929" checksum="2A7CBA94A29304F1" modified="2024-01-24" version="1">MISERKQSKYVCVIGAGPSGLVTTRELRKEGHCVVMMEQNHDVGGQWLYDPNVEGEDPLGRSKFSEVHSSIYASLRLASPREIVGFSDFPFVVKKGRDTRRFPGHRELLWYLEDFCECFGLRETIRFNTRVEYVGMLDSDEVGEGLKWVVRSRDVESEKVVEEVFDAVVVATGQYSHPRLPSIKGMEAWKRKQMHSHIYRVPHPFHNEVVVVVGNAASGQDICIELVGVAKEIHLSAKSLDISEGLSKIISKHHNLHLHPQIESLHEDGRVVFVDGSWVVADTIIYCTGYSYSFPFLDTKGIVAVDDDRVGPLYEHTFPPSLAPSLSFVGIPRKIIAFPFFESQAMWIAQLLSGRKTLPSFHDMVRSIEDFYQSRDAAGIPKHQTHDIADFEYCDKYGDNVGFPHTEEWRKKLLLSTVGNAQVNLETYRDSWYDHELLQVVLQSPLFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DCU7</accession>
    <name>A0AA39DCU7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_021338</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DCU7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47447">
        <property type="entry name" value="OS03G0856100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47447:SF28">
        <property type="entry name" value="PENTACOTRIPEPTIDE-REPEAT REGION OF PRORP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13812">
        <property type="entry name" value="PPR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="192"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="297"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="332"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="367"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="402"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="437"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="472"/>
            <end position="506"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9679376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="518" mass="59192" checksum="F935E9694F17F112" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DED3</accession>
    <name>A0AA39DED3_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_021159</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DED3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00113">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010417">
        <property type="entry name" value="Embryo-specific_ATS3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036392">
        <property type="entry name" value="PLAT/LH2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31718:SF31">
        <property type="entry name" value="OS01G0172800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31718">
        <property type="entry name" value="PLAT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06232">
        <property type="entry name" value="ATS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49723">
        <property type="entry name" value="Lipase/lipooxygenase domain (PLAT/LH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041227498" evidence="1">
        <location>
            <begin position="20"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9679147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="167" mass="18871" checksum="C9018D86EC9B1395" modified="2024-01-24" version="1">MKGVYFLLHLASIFIISQAQSIVSHPHALKSQNFNSIQKVGRTCSFIVIIKTSCSSIQFTRDQISLAFGDAYGNKVYAPRIDDPTSRRFERCVSDTFQISGVCTYQICHVAFYRSGSDGWKPESVTIYGFNSRPVTFYYKRFIPRGVWFGFNHCSKASGFIITSTSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DF37</accession>
    <name>A0AA39DF37_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Alkaline/neutral invertase</fullName>
            <ecNumber evidence="6">3.2.1.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_017884</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Invertase that cleaves sucrose into glucose and fructose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.</text>
            <dbReference type="EC" id="3.2.1.26"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the glycosyl hydrolase 100 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.26" evidence="6"/>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9681716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DF37"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033926">
        <property type="term" value="F:endo-alpha-N-acetylgalactosaminidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004575">
        <property type="term" value="F:sucrose alpha-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005987">
        <property type="term" value="P:sucrose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000001">
        <property type="entry name" value="probable alkaline/neutral invertase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024746">
        <property type="entry name" value="Glyco_hydro_100"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31916">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31916:SF46">
        <property type="entry name" value="ALKALINE_NEUTRAL INVERTASE A, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12899">
        <property type="entry name" value="Glyco_hydro_100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5 6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9681716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="673" mass="76567" checksum="0402AD8CCB8C1984" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DJ52</accession>
    <name>A0AA39DJ52_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">S-locus receptor kinase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_015582</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DJ52"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27006:SF606">
        <property type="entry name" value="INTERLEUKIN-1 RECEPTOR-ASSOCIATED KINASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27006">
        <property type="entry name" value="PROMASTIGOTE SURFACE ANTIGEN PROTEIN PSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9686798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="89" mass="9698" checksum="FF0DA7F94DA16CCA" modified="2024-01-24" version="1">MDQTLSETCNTKEFLRCVNVGLLCVQEDPSDRPTVAVAVVMLSSDIATMPVPKQPAFVLKRDLSRTASSSSKPEASWNSEILATIEEGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DJE4</accession>
    <name>A0AA39DJE4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Cellulose synthase-like protein H1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_015395</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DJE4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016760">
        <property type="term" value="F:cellulose synthase (UDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030244">
        <property type="term" value="P:cellulose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000145">
        <property type="entry name" value="Cellulose synthase-like protein H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005150">
        <property type="entry name" value="Cellulose_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13301">
        <property type="entry name" value="X-BOX TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03552">
        <property type="entry name" value="Cellulose_synt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="46"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="578"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="617"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="671"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="135"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="468"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR605150-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR605150-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR605150-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9686471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="755" mass="84478" checksum="A40382F2EA9040DD" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DN06</accession>
    <name>A0AA39DN06_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Receptor-like protein 51</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_013071</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DN06"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001678">
        <property type="entry name" value="Calmodulin-binding receptor kinase CaMRLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053038">
        <property type="entry name" value="RLP_Defense"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48064">
        <property type="entry name" value="OS01G0750400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48064:SF1">
        <property type="entry name" value="RECEPTOR-LIKE PROTEIN 51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041379936" description="Receptor-like protein 51" evidence="3">
        <location>
            <begin position="28"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="414"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="373"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="373"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="387"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="400"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9690728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="440" mass="47445" checksum="0F22813693750BC3" modified="2024-01-24" version="1">MDPHPPPFSYFVTLLYLLLFSVTLTSSLSPPISPTKSPSAPSPLPSASSSSPSSSSTLDPKQLRALQSLNIPTSKDPCAQPSIHNATLCDSSKPFRHLLSLRLVNCSDDVALSSTALKSLSTLQDLEFINCPVAAVHFPSDLLSSLRSLTCIRSLRRLTGVWLSRFQNLTDLTVSNVHVNASGPFVILNSMKKLRSVTISHTNLTGYLPKHWHLNITHLDLSGNELIGKIPSAVTLLENLQLLNLSSNSLTGEIPSTLGDLLSLQNMSLASNSLSGPIPESMAAMPSLVHIDLSFNQLNGTIPDYLTKMKGLKYLNLENNNFNGVMPFNVSFIKKLVVFKVGGNSNLCYNHSLISSKMKLGIAPCDKHGLPISPAPAKSDSSADESGSSDDSDYNDSEDDSSHKEEHHHGPNKVVLGVAIGLSSIVFLIVFLILLSKWCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DQ26</accession>
    <name>A0AA39DQ26_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_013682</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DQ26"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="249"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="252"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9691570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="343" mass="39521" checksum="DC57FA93EC501729" modified="2024-01-24" version="1">MAPKRAPAEVSSTANKGKRLEVAKMTGSKRKRLDKAQRNANKGKRVVIDENVEARKKATFNRATFITLELAQRFNLHFHNRTIIPGRNIDFSKLSYFQFDRLFMRMGWLPIVSLKEFLYPRRLCGYKKFGRPTSHSLTVLSRILQYMISYIFIPKRGHRDDVSFLEVFLVDSILTERKINMGYIIFRHMKASSLSEDSVLPYGMFITKIIKYFNVNLRNKTDRKKLKSFDTCDWVSLRCMHFSYVPPSEVDVSSDDESSQDEEYENQDVEGRGFENANVTTIPSNDGVGAIVVADHPSQIGRNDAEASDNLNAQIYSLGTHLEEMVLANERRFTCLEDRIDGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DQN3</accession>
    <name>A0AA39DQN3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">PVL29_011308</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="25">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the leguminous lectin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="25">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002229">
        <property type="term" value="P:defense response to oomycetes"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000240">
        <property type="entry name" value="Lectin-domain containing receptor kinase A4.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000129">
        <property type="entry name" value="Leucine-rich repeat receptor-like kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000146">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase IOS1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024788">
        <property type="entry name" value="Malectin-like_Carb-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45631">
        <property type="entry name" value="OS07G0107800 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45631:SF206">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12819">
        <property type="entry name" value="Malectin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 22" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="13 22" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Protein kinase" evidence="24">
        <location>
            <begin position="521"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="24">
        <location>
            <begin position="1180"/>
            <end position="1454"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="24">
        <location>
            <begin position="1556"/>
            <end position="1836"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="822"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="1464"/>
            <end position="1514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="824"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="1464"/>
            <end position="1473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="1492"/>
            <end position="1509"/>
        </location>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="1209"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="KAJ9692170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1845" mass="205122" checksum="04E18E49E2C7598A" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DRK3</accession>
    <name>A0AA39DRK3_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_013744</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DRK3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9691642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="134" mass="14969" checksum="C6966BBA986D008C" modified="2024-01-24" version="1">MPTGFSDKSLSTTTCIRRLTAVLLGEFVLYNKSSGNGEIEFVETLACCVFNTEETFVRIVMSNGTCTAVLMGHSYLVKEVTRDPIGSFIASQIDDIIVFIKTMLHLIYSSRNFAFLERRLFGFIMSGQLTETDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DTF7</accession>
    <name>A0AA39DTF7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Histidine-containing phosphotransfer protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_008521</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Functions as a two-component phosphorelay mediators between cytokinin sensor histidine kinases and response regulators (B-type ARRs). Plays an important role in propagating cytokinin signal transduction.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
            <location evidence="7">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">Histidine-containing phosphotransfer domain (HPt) contains an active histidine that mediates the phosphotransfer.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DTF7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009927">
        <property type="term" value="F:histidine phosphotransfer kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043424">
        <property type="term" value="F:protein histidine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009736">
        <property type="term" value="P:cytokinin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00088">
        <property type="entry name" value="HPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.160:FF:000001">
        <property type="entry name" value="Histidine-containing phosphotransfer protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.160">
        <property type="entry name" value="HPT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045871">
        <property type="entry name" value="AHP1-5/YPD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036641">
        <property type="entry name" value="HPT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008207">
        <property type="entry name" value="Sig_transdc_His_kin_Hpt_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28242:SF13">
        <property type="entry name" value="HISTIDINE-CONTAINING PHOSPHOTRANSFER PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28242">
        <property type="entry name" value="PHOSPHORELAY INTERMEDIATE PROTEIN YPD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01627">
        <property type="entry name" value="Hpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47226">
        <property type="entry name" value="Histidine-containing phosphotransfer domain, HPT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50894">
        <property type="entry name" value="HPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2 7" id="KW-0932">Cytokinin signaling pathway</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="HPt" evidence="8">
        <location>
            <begin position="38"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphohistidine" evidence="6">
        <location>
            <position position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU369004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9696338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="151" mass="17429" checksum="2B396BBC4E68680D" modified="2024-01-24" version="1">MDVGQLQRQFTDYTASLFREGFLDDQFTQLQKLQDENNPDFVFEVVSLFFDDSEKLVNNMAKALEQQTVEFKQVDAHVHQFKGSSSSVGAHRVKNVCVTFRNFCEQQNIEGCKRCLQQLRHEYSLLKSKLETLFRLEQQIIAAGGSIPMVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DUR8</accession>
    <name>A0AA39DUR8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PVL29_010364</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DUR8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009742">
        <property type="term" value="P:brassinosteroid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000016">
        <property type="entry name" value="probable serine/threonine-protein kinase At4g35230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000154">
        <property type="entry name" value="probable serine/threonine-protein kinase At4g35230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000069">
        <property type="entry name" value="probable serine/threonine-protein kinase At5g41260"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045845">
        <property type="entry name" value="BSK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025749">
        <property type="entry name" value="Sphingomyelin_synth-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058209">
        <property type="entry name" value="TPR_BSK1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45863">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE BSK5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45863:SF6">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE BSK6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14360">
        <property type="entry name" value="PAP2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25575">
        <property type="entry name" value="TPR_BSK1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1070">Brassinosteroid signaling pathway</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0519">Myristate</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="109"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="169"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="225"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="388"/>
            <end position="642"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ9694852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="820" mass="92775" checksum="5D79BA2A3303F481" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DYL1</accession>
    <name>A0AA39DYL1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_004565</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclic nucleotide-gated cation channel (TC 1.A.1.5) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DYL1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005216">
        <property type="term" value="F:monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.630:FF:000003">
        <property type="entry name" value="Cyclic nucleotide-gated ion channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000024">
        <property type="entry name" value="Cyclic nucleotide-gated ion channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.630">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45651:SF9">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-GATED ION CHANNEL 14-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45651">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-GATED ION CHANNEL 15-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0407">Ion channel</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="99"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="133"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="191"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="216"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="260"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="388"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="12">
        <location>
            <begin position="492"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="660"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="660"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="713"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="726"/>
            <end position="735"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9702881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="735" mass="85163" checksum="4EA45548C969D133" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DYP9</accession>
    <name>A0AA39DYP9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_006918</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DYP9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080043">
        <property type="term" value="F:quercetin 3-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080044">
        <property type="term" value="F:quercetin 7-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000019">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000429">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058980">
        <property type="entry name" value="Glyco_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926">
        <property type="entry name" value="GLUCOSYL/GLUCURONOSYL TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926:SF1560">
        <property type="entry name" value="UDP-GLYCOSYLTRANSFERASE 74E1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26168">
        <property type="entry name" value="Glyco_transf_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase N-terminal" evidence="5">
        <location>
            <begin position="11"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF26168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9701753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="450" mass="51325" checksum="CF2C633BB6195C9F" modified="2024-01-24" version="1">MKRGERVSETHIMVLPYPSQGHINPMLQFSRRLASKGLKVTLVIPRASIYNAQASSINIEIICEGLEERKEEESIEDYAERFRVVVSQSLAELIKKHSRSSHPAKFLVYDSMMPWAQDVAEPLGLDGVPFFTQSCAVSTIYYHFNQGKLKTPLEGYTVSIPSMPLLCINDLPSFINEKTILSFLLKQFSNFQKVKWILFNTFDKLEDEVMKWMASLRPIKTIGPTVPSMYLDKRLEEDKDYCLSLFKQNVDAYIAWLDTKGIGSVVYVSFGSMASLGEEQMEEIAWGLKRSNTHFMWVVRELEEKKLPCKFLEETCEKGLVVSWCSQLEVLSHKAVGCFMSHCGWNSTLEALSLGVPMIAMPHFSDQTTNAKFIEDVWGIGVRVKPDEKGLVKREEIEMCIREMMEGERGNEMRRNAEMWKELAKEAVSEGGTSDKNIDEFIAEILCSLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DZF8</accession>
    <name>A0AA39DZF8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Bicarbonate transporter-like transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_004306</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the anion exchanger (TC 2.A.31.3) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DZF8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005452">
        <property type="term" value="F:solute:inorganic anion antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006820">
        <property type="term" value="P:monoatomic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050801">
        <property type="term" value="P:monoatomic ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.570">
        <property type="entry name" value="Helical hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011531">
        <property type="entry name" value="HCO3_transpt-like_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003020">
        <property type="entry name" value="HCO3_transpt_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453">
        <property type="entry name" value="ANION EXCHANGE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453:SF40">
        <property type="entry name" value="BORON TRANSPORTER 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00955">
        <property type="entry name" value="HCO3_cotransp"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="121"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="154"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="196"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="229"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="291"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="331"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="467"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="546"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="domain" description="Bicarbonate transporter-like transmembrane" evidence="7">
        <location>
            <begin position="7"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="Bicarbonate transporter-like transmembrane" evidence="7">
        <location>
            <begin position="201"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="Bicarbonate transporter-like transmembrane" evidence="7">
        <location>
            <begin position="453"/>
            <end position="595"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9702503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="660" mass="73967" checksum="3122A3098DF5A83A" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E089</accession>
    <name>A0AA39E089_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF1764 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_003933</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9701935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E089"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013885">
        <property type="entry name" value="DUF1764_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34066:SF1">
        <property type="entry name" value="DUF1764 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34066">
        <property type="entry name" value="GROWTH FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08576">
        <property type="entry name" value="DUF1764"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9701935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="156" mass="17326" checksum="8404D88B3DEC9569" modified="2024-01-24" version="1">MPKKSSAKKPKSKQASPDVEQKKPTPIVKEPGNEIDEIFSGKKRKTPEKESPDVEEEKPTPAVKKSTNEIDEIFAGKKKKKPKQEQPKKGIEGVHSKLKNKKKSKRDEEGDFSDPPSRARKKTEDGLILYTEEELGIGKSDAGGTPLCPFDCSCCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E1E5</accession>
    <name>A0AA39E1E5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">H15 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_005111</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the histone H1/H5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E1E5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031492">
        <property type="term" value="F:nucleosomal DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030261">
        <property type="term" value="P:chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045910">
        <property type="term" value="P:negative regulation of DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00073">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005819">
        <property type="entry name" value="H1/H5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005818">
        <property type="entry name" value="Histone_H1/H5_H15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11467:SF36">
        <property type="entry name" value="HISTONE 24-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11467">
        <property type="entry name" value="HISTONE H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00538">
        <property type="entry name" value="Linker_histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00624">
        <property type="entry name" value="HISTONEH5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00526">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51504">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="4 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="H15" evidence="8">
        <location>
            <begin position="48"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="118"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="33"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="127"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="151"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9703674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="187" mass="20330" checksum="F9D8250FFEB830E5" modified="2024-01-24" version="1">MSTEGVAVEQPAQEVAVTEQAVAPEMPAPEKKPKARKEKKPKQARASSHPPYFQMIKEALLALDDKSGSSPYAIAKHMEEEHKAVLPANFRKILSLQLKNSVAKGNLIKIKASYKLSGVGKGTAKSDGAKRAKATSDPKKKQVRKSETPKKAPKKVAAKKTRKTVPSKLKQPRSIRSSAKKAKKVTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E1G4</accession>
    <name>A0AA39E1G4_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_002961</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E1G4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990547">
        <property type="term" value="P:mitochondrial phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000070">
        <property type="entry name" value="Mitochondrial phosphate carrier protein 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044677">
        <property type="entry name" value="SLC25A3/Pic2/Mir1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45671:SF4">
        <property type="entry name" value="MITOCHONDRIAL PHOSPHATE CARRIER PROTEIN 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45671">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25 (MITOCHONDRIAL CARRIER PHOSPHATE CARRIER), MEMBER 3, LIKE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="15"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="108"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="209"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9704663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="308" mass="33920" checksum="4796BA9864282E3F" modified="2024-01-24" version="1">MMRVEGRAYQELSPGYYGICTVGGMLSAGTTHLAITPLDVLKVNMQVNPIKYNSIPSGLNTLWKEQGPSALWRGWSGKLFGYGIQGGCKFGLYEYFKRIYSDVLVDQNRSVIFFASSASAQVFADVALCPFEAVKVRVQTQPNFAKGLADGFPKLYATEGLSGFYKGLFPLWGRNLPFSMTMFSTFEHSIDFIYNNIIQKKKEDCSVAQQLGVTCLAGYVAGAFGTIISNPADNIVSSLYNKKAENVLQAVKNIGFVNLFTRSLPLRITFVGPVVTLQWFFYDTIKVLSGLPTSGGPVRHIEEADRSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E1U4</accession>
    <name>A0AA39E1U4_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_005046</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E1U4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9703540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="95" mass="10853" checksum="BBD68BE866641A1E" modified="2024-01-24" version="1">MGKLILVRCTCTFRNIKLVMIKQPSPVECWSSTLSHGNSDIYELSRLSGHAISNNPLVPAEHYLYYSITEVAYKQWLRHCSDQLGHIVPTIFMSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E4X0</accession>
    <name>A0AA39E4X0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_003309</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E4X0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000361">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="77"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9705187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="159" mass="17525" checksum="BE6DA7EEFF2D8BB0" modified="2024-01-24" version="1">MLRNGTLPDNYTFPFVLKACARLSLLRKGREIHSSTLKLWVHLDVFLQNALISAFSSCGAVEAACAVFDMLSALVRDVVSWNCMISGYLESHRYELALKVFWELLGDGSLSPDEVTLVSALSVCGRLGLLDLGKKIHGLFMGNGFVLDVFLGSSYQYVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YGF9</accession>
    <name>A0AA38YGF9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">mitogen-activated protein kinase kinase kinase</fullName>
            <ecNumber evidence="2">2.7.11.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_026514</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.25" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9669991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YGF9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004709">
        <property type="term" value="F:MAP kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000357">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050538">
        <property type="entry name" value="MAP_kinase_kinase_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008808">
        <property type="entry name" value="Powdery_mildew-R_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016">
        <property type="entry name" value="MAP KINASE KINASE KINASE SSK2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016:SF5">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05659">
        <property type="entry name" value="RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="287"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="203"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="203"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="316"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9669991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="567" mass="64026" checksum="01D00E977E60E5C6" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YI69</accession>
    <name>A0AA38YI69_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WRKY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_027059</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YI69"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.80:FF:000003">
        <property type="entry name" value="WRKY transcription factor 57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.80">
        <property type="entry name" value="WRKY domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003657">
        <property type="entry name" value="WRKY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036576">
        <property type="entry name" value="WRKY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044810">
        <property type="entry name" value="WRKY_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31221:SF283">
        <property type="entry name" value="WRKY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31221">
        <property type="entry name" value="WRKY TRANSCRIPTION FACTOR PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03106">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00774">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118290">
        <property type="entry name" value="WRKY DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50811">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="WRKY" evidence="6">
        <location>
            <begin position="100"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9670889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="193" mass="21342" checksum="E1439CE80DC76352" modified="2024-01-24" version="1">MPQPFHSTIPPPAMDFPPPLNPNHIFINSSFAPLSSEFEPSDYLLLDDASDDVAAPVAVTGGSSIGATPMMNTTTNMECKDGVKRKKTDLGFRVAFKTKSDLEIMDDGFKWRKYGKKSVKNSPNPRNYYKCASGGCNVKKRVERDREDSSYVITTYEGVHNHESPCVVYYNQVPLMVSSNAWTLQASSQSSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YIK6</accession>
    <name>A0AA38YIK6_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_027213</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9671113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YIK6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9671113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="94" mass="10470" checksum="81DFCE4A5346FD2B" modified="2024-01-24" version="1">MPLPETVEPVARNKWELVMVPFQLANRGLVRGLLGALVDLQHGALLMGKGLKNSPLIHHVGNHRHRLWRLRLLMVHACELCFVAAPVPAQCIIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YJE9</accession>
    <name>A0AA38YJE9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA exonuclease 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_025305</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Exoribonuclease involved in ribosome biosynthesis. Involved in the processing of ITS1, the internal transcribed spacer localized between the 18S and 5.8S rRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the REXO4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YJE9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06144">
        <property type="entry name" value="REX4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000007">
        <property type="entry name" value="Interferon-stimulated exonuclease gene 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037431">
        <property type="entry name" value="REX4_DEDDh_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047021">
        <property type="entry name" value="REXO1/3/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Ribonucl_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801:SF45">
        <property type="entry name" value="RNA EXONUCLEASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801">
        <property type="entry name" value="RNA EXONUCLEASE REXO1 / RECO3 FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Exonuclease" evidence="11">
        <location>
            <begin position="81"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="243"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="63"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="249"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9671538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="277" mass="31746" checksum="B01FEA67B4BF4AE1" modified="2024-01-24" version="1">MGTQQRNPKPNSHHQLNPNWTQLQQKLKLHGSRPSQKSSDGAEPQTPKTILGKRKERPDTEPDASQRNVLIPTNTDSSLTDAVAMDCEMVGVGSEGNKSALGRVTLVNKWGNVIYDEYVHPVEWVVDFRTEISGIRPRDLKKAKDFQTVQRQVAELIKGRLLVGHALRNDLKALLLSHPKVDMRDTSECELFLKEERRRVALRHLAAEFLGVKIQNGEHCPVEDARAAMLLYQRNRKQWEKNIKDQIRLRQKQKKRKQKKKPKRRDASDVNHAARAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YMK2</accession>
    <name>A0AA38YMK2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PhoD-like phosphatase metallophosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_023036</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YMK2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07389">
        <property type="entry name" value="MPP_PhoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.70">
        <property type="entry name" value="PhoD-like phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018946">
        <property type="entry name" value="PhoD-like_MPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038607">
        <property type="entry name" value="PhoD-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33987">
        <property type="entry name" value="CALCINEURIN-LIKE METALLO-PHOSPHOESTERASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33987:SF1">
        <property type="entry name" value="CALCINEURIN-LIKE METALLO-PHOSPHOESTERASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09423">
        <property type="entry name" value="PhoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041231996" description="PhoD-like phosphatase metallophosphatase domain-containing protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="425"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="PhoD-like phosphatase metallophosphatase" evidence="3">
        <location>
            <begin position="43"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9673190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="461" mass="51872" checksum="763D232FAC1EAFDF" modified="2024-01-24" version="1">MGRSVLELWLLWSVVMRVMIALPIAYSASSTSSQEALLVSRIAFGSCANQSAPQPIWNAIIDFDPQIFIWLGDNIYGDVRHPFKLFGKERTIGPWKNVPRFIPSSEQEMQSRYKKTKTNPGYSRLLEKTKVIGTWDDHDYGLNDAGKEFNGKITSQRLLLDFLDEPQDSPRRKQAGVYTSYTFGPVGRQIKVILLDTRYHRDPLFSDGSILGSSQWTWLESELKGPASALTIIGSSIQVISNLSAITGPIFYLECWGRFPKERERLFKLIADSKRDGVFFISGDVHFGEITRYDCATGYPLFDITASGITQAVEKAVPPPLNFIVRFVAWLAPTTMRVMGQHCRHKSCTYGQPNFGAIEIDWDATPVTVKMEVRDVNGFPVLSVNTSLLELKARSVGALYSIKPGQYRKHCSLEVTLPWIVRHRLAILFFCALAMLLLTLAGLTFAAISVIRRCLCKDKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YPL3</accession>
    <name>A0AA38YPL3_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_023705</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YPL3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000123">
        <property type="term" value="C:histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990841">
        <property type="term" value="F:promoter-specific chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048586">
        <property type="term" value="P:regulation of long-day photoperiodism, flowering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18983">
        <property type="entry name" value="CBD_MSL3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.274.30:FF:000005">
        <property type="entry name" value="Chromatin modification-related protein EAF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000102">
        <property type="entry name" value="Protein MRG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.274.30">
        <property type="entry name" value="MRG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008676">
        <property type="entry name" value="MRG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038217">
        <property type="entry name" value="MRG_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026541">
        <property type="entry name" value="MRG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053820">
        <property type="entry name" value="MSL3_chromo-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10880">
        <property type="entry name" value="MORTALITY FACTOR 4-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10880:SF44">
        <property type="entry name" value="PROTEIN MRG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05712">
        <property type="entry name" value="MRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22732">
        <property type="entry name" value="MSL3_chromo-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51640">
        <property type="entry name" value="MRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="291"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="MSL3 chromodomain-like" evidence="9">
        <location>
            <begin position="41"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="MRG" evidence="8">
        <location>
            <begin position="136"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF05712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF22732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9674311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="321" mass="37295" checksum="229801CEC9FC760F" modified="2024-01-24" version="1">MGSSVDASATETDGDTTETDIDVDADSDADDYPPLSDSCPFSEGEKVLAYHNLRIYPAKVRRIEFQMNEWKYFVHYLGWSKNWDEWVGMDRLLKFSEENVQKQKALGKKQGIDKNTKPVRASQIKPKNFARGKKWKNDSVAKKEAIPVEKLVNIQIPPTLKKQLVDDCEFITHLGQLVRLPRAPTVDKILKKYLDYRIKRDGMISDSAGEILKGLRCYFDKALPVMLLYERERQQYQEAIANNVSPSTIYGAEHLLRLFVKLPELLFHANIEKETSKELQLELLDFLKYKFFHSFTLIYFLLVMFLTAIVLAIKWDYHENM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YQ57</accession>
    <name>A0AA38YQ57_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_023834</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thioredoxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YQ57"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43601:SF11">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43601">
        <property type="entry name" value="THIOREDOXIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="3">
        <location>
            <begin position="99"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9674517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="217" mass="24568" checksum="CBFDD3145FEDBFB0" modified="2024-01-24" version="1">MGSTPKQPLFCLKWPWDVHQNSKNPHGCSFETPWVLKSLQNLGSVAFNLYSSVSRSSYPWIRTFKPFQLDVGVSQKTISKMGKKILTPEEQGEAEQRALASALASRKVATVIEFYSPNCRLCTSLLGFVLEVESKNSDWLTIVMADAENDKWLPELLHYDIRYVPCFVLLDKYGRALAKTGIPNSRLHVIAGVSHLLKMKRPQKIEHQFSTSDPDNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YT51</accession>
    <name>A0AA38YT51_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF4216 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_024948</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9676200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YT51"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025312">
        <property type="entry name" value="DUF4216"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48258">
        <property type="entry name" value="DUF4218 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48258:SF3">
        <property type="entry name" value="FK506-BINDING PROTEIN 4-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13952">
        <property type="entry name" value="DUF4216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4216" evidence="1">
        <location>
            <begin position="2"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9676200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="126" mass="14467" checksum="963F0184E8508795" modified="2024-01-24" version="1">MFRIPVFKCDWVDNRNGIKVDDLGFTLVDFNKTAHKSDPFILASQAKQVFYVQDQHDPRWSVVLSTPQKDFLDQERGDDLVDNFVEHHPFIGALPQVEAFDAMDDSDAICMRGDCEGIWIENKSSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YV14</accession>
    <name>A0AA38YV14_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sulfotransferase</fullName>
            <ecNumber evidence="3">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_022298</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YV14"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11783">
        <property type="entry name" value="SULFOTRANSFERASE SULT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="5">
        <location>
            <begin position="82"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9677221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="350" mass="40738" checksum="E2FF9A960D434BD9" modified="2024-01-24" version="1">MTTVPSSQKLLMPGTSGGEEQEQQNHERERSFERYREIISTLPKEKGWVTEHLYQYQGCWYRTPSAIQGVIWMQEHFKPRPADVLLVTPPKSGTTWFKSLLFAIMNRTQFDTSTHPLLTTSPHELVPFMEMFLHMNIPFPDPDPLSPPRLFHTHTPFTSLPQSVIDSQCRIVYVSRNPKDVFVSFYCFLQGDNNSAIRLSFEEAFEQYCKGFSPYGPFWDHILGYWKASSKWPERVLLLKYEDMKRDSSFHLKRTAEFIGQPFSSEEEKQGLVHEIIKLCSFESLSNMKVNKTGTLRVGYLTVDKNSFFRKGEVGDWKNHLTAEMAERLDRIVERNLDGCDFSFDDSKDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YWZ2</accession>
    <name>A0AA38YWZ2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BRISC and BRCA1-A complex member 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_022813</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9678042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YWZ2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070552">
        <property type="term" value="C:BRISC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045739">
        <property type="term" value="P:positive regulation of DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21502">
        <property type="entry name" value="vWA_BABAM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026126">
        <property type="entry name" value="BABAM1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15660">
        <property type="entry name" value="BRISC AND BRCA1-A COMPLEX MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15660:SF1">
        <property type="entry name" value="BRISC AND BRCA1-A COMPLEX MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9678042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="225" mass="25520" checksum="29D79A3A3D998361" modified="2024-01-24" version="1">MEGMEAQSSSPFAYALNPSHLFNKDILLRIDVDVESMVEMKVTGSKGRPITSMYSIKQAIFLFVHARLAINSDHRFAFAALGKTTSWLQREFSSEFDSAIAALRSLLVDGSCGNANLTQLFRHQWPVNLKLFTLDTVYLHNKPGLENCPQKVYDALVNTLEHVSEHEGHTRESRQGLTRVLFHYMCVLSSYPQQRCQPDDVNIPKPLTKEAVIFTTWVVIMHHPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z004</accession>
    <name>A0AA38Z004_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_021683</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z004"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000590">
        <property type="entry name" value="PR5-like receptor kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045874">
        <property type="entry name" value="LRK10/LRL21-25-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27009">
        <property type="entry name" value="RUST RESISTANCE KINASE LR10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="145"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="209"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9679848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="501" mass="57116" checksum="26E0E2647366EC97" modified="2024-01-24" version="1">MNCTRPISAHNYIQITPCDSNNGSSFSSETYAYALVGDSLKVRDNIQYSCTIGLTVIAPIWKGVSEPRNRSMSNLQENLLMGLELSYSPVNCSAALHVEKIEENLWSFFDRRVDIILRNIFPDRYYLTNYGSYESYPHVLVKGEWILHIIVLIIGGRYVLGILCLLAYLIYKFHRRHLSLDDSIEEFLQKHKNLQPIRYSYSEIKKMTNNFKTPLGKGGFGFVYKGKLQSGSIVAVKVLATSKANGEDFINEVATIGRIHHMNVYIFLERENSVYLSWEMLYKIALGIARGIEYLHRGCDMQILHFDIKPHNILLEDFTPKVSDFGLAKLYSTDESIVSLTAARGTLGYIASELFYKNIGGISYKADVYSFGMLLMEMVGRRKNVKALPENSNQIYFPSWIHGRYSRGENMEMGDATEDEKKLVKKMVLVALWCIQLKPTNRPSMGKALEMLEGEVELLQMPPKPTLYYEEMSIEKQINSPIRVQPISSCNAMDTISLDGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z1T1</accession>
    <name>A0AA38Z1T1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Chalcone/stilbene synthase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_019829</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thiolase-like superfamily. Chalcone/stilbene synthases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z1T1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030639">
        <property type="term" value="P:polyketide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012328">
        <property type="entry name" value="Chalcone/stilbene_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011141">
        <property type="entry name" value="Polyketide_synthase_type-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11877">
        <property type="entry name" value="HYDROXYMETHYLGLUTARYL-COA SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11877:SF46">
        <property type="entry name" value="TYPE III POLYKETIDE SYNTHASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02797">
        <property type="entry name" value="Chal_sti_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Chalcone/stilbene synthase C-terminal" evidence="3">
        <location>
            <begin position="7"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9680627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="106" mass="12182" checksum="22CF97E3FB979642" modified="2024-01-24" version="1">MLAAGRDTLKLRSEKLECRRRALMDYGNVSSNTIFYVMEYMREGLKRKGGEEWGLALAFGPGITFQRNSDSKPVTTDNLHAYMLHFHLLSSMDLYVMECLGLWTLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z368</accession>
    <name>A0AA38Z368_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_020240</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9681267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z368"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009640">
        <property type="term" value="P:photomorphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009585">
        <property type="term" value="P:red, far-red light phototransduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000090">
        <property type="entry name" value="E3 ubiquitin-protein ligase RFWD2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044630">
        <property type="entry name" value="SPA1/2/3/4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44218">
        <property type="entry name" value="PROTEIN SPA1-RELATED 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44218:SF6">
        <property type="entry name" value="PROTEIN SUPPRESSOR OF PHYA-105 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0607">Phytochrome signaling pathway</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="2 9" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="166"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="854"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="940"/>
            <end position="973"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00084091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9681267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1079" mass="119736" checksum="15DFE4B6324CE31B" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z3J9</accession>
    <name>A0AA38Z3J9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">Nicotianamine synthase</fullName>
            <ecNumber evidence="2 6">2.5.1.43</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_017909</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Synthesizes nicotianamine, a polyamine which serves as a sensor for the physiological iron status within the plant, and/or might be involved in the transport of iron.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>3 S-adenosyl-L-methionine = nicotianamine + 3 S-methyl-5'-thioadenosine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:16481"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:58249"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.5.1.43"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the nicotianamine synthase (NAS)-like family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.43" evidence="2 6"/>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9681756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z3J9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030410">
        <property type="term" value="F:nicotianamine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030418">
        <property type="term" value="P:nicotianamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004298">
        <property type="entry name" value="Nicotian_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32266">
        <property type="entry name" value="NICOTIANAMINE SYNTHASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32266:SF12">
        <property type="entry name" value="NICOTIANAMINE SYNTHASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03059">
        <property type="entry name" value="NAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51142">
        <property type="entry name" value="NAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9681756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="308" mass="34545" checksum="EBAED45F448E188A" modified="2024-01-24" version="1">MGCQDELLVEKVCEMYEKISRLESLKPSKDVNMLFTQLVLTCMPPSPIDVTKLCKRVQEMRSKLIRLCGEAEGLLEGHFSTILGSYENPLDHLNLFPYYSNYLKLSLLEFTLLSQNCTHVPGKIAFVGSGPLPLTSIVLASYHLTTTSFHNYDIDPAANSMAAHLVSSDPDFSKRMLFHTTDIMNVTNELKEYDVVYLAALVGMDKEEKIQVMDHLAKYMAPGALLMLRSAHGARAFLYPVIDPCDLRGFEVLSIFHPTDEVINSVVVARKFPMHVHSLDQGLGSMILPSKCSEIQVFNPLEELTIEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z4V2</accession>
    <name>A0AA38Z4V2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AT-hook motif nuclear-localized protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_018357</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Transcription factor that specifically binds AT-rich DNA sequences related to the nuclear matrix attachment regions (MARs).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z4V2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003680">
        <property type="term" value="F:minor groove of adenine-thymine-rich DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11378">
        <property type="entry name" value="DUF296"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.80:FF:000001">
        <property type="entry name" value="AT-hook motif nuclear-localized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.80">
        <property type="entry name" value="Hypothetical protein, similar to alpha- acetolactate decarboxylase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014476">
        <property type="entry name" value="AHL15-29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005175">
        <property type="entry name" value="PPC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31100">
        <property type="entry name" value="AT-HOOK MOTIF NUCLEAR-LOCALIZED PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31100:SF3">
        <property type="entry name" value="AT-HOOK MOTIF NUCLEAR-LOCALIZED PROTEIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03479">
        <property type="entry name" value="PCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016021">
        <property type="entry name" value="ESCAROLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117856">
        <property type="entry name" value="AF0104/ALDC/Ptd012-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51742">
        <property type="entry name" value="PPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="PPC" evidence="8">
        <location>
            <begin position="91"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="218"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="232"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR016021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9682425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="290" mass="29645" checksum="AD7DCB19BFD1DF10" modified="2024-01-24" version="1">MANRWWAGQVGLQGVDTSSASPAMKKPDLGISMNENGGSGSGGGGEEEEEKENSDEPREGAIEVATRRPRGRPPGSKNKPKPPIFVTRDSPNALRSHVMEVANGSDITESIAQFARRRQRGVCVLSASGTVMNVTLRQPSAPGGAVMALHGRFEILSLTGAFLPGPAPPGSTGLTIYLAGGQAQVVGGSVVGSLIAAGPVMVIAATFSNATYERLPLEDEEEAGSAAQEQLAGGGGGGSPPGIGGSGGQQQAGMADPSSMPVYNLPPNLLPNGGQLNHDAYGWAHGRQPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z6D8</accession>
    <name>A0AA38Z6D8_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_019011</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z6D8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009791">
        <property type="term" value="P:post-embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000129">
        <property type="entry name" value="Leucine-rich repeat receptor-like kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000233">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase TDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000041">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046956">
        <property type="entry name" value="RLP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063">
        <property type="entry name" value="LRR RECEPTOR-LIKE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063:SF16">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 14" id="KW-0732">Signal</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041461689" evidence="14">
        <location>
            <begin position="20"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="920"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="15">
        <location>
            <begin position="43"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="16">
        <location>
            <begin position="291"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="886"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="902"/>
            <end position="913"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9683251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="985" mass="108907" checksum="634E400B12658AE8" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z757</accession>
    <name>A0AA38Z757_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_019303</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z757"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057075">
        <property type="entry name" value="bHLH_IRO3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47075">
        <property type="entry name" value="TRANSCRIPTION FACTOR BHLH47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47075:SF9">
        <property type="entry name" value="TRANSCRIPTION FACTOR BHLH47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23177">
        <property type="entry name" value="bHLH_IRO3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="7">
        <location>
            <begin position="33"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="133"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="193"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="23"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="141"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="210"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="232"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9683670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="244" mass="26821" checksum="9C0D67487EB598A6" modified="2024-01-24" version="1">MVSEVSSGNDGCVAGERVVGRPSPRKKNPGKVPKKIHKAEREKLKRDHLNVLFLELGNTLESVQQNNGKACVLTDATRLLRDLLTQVDCLKRDNAALLSESHYVTMEKNELREDNSALEAQIKKLQSELEERIRSKPAWNSDPSQLDHNSTTPHLPDDHLMLPVTDHASQTAPVVGPVFVVPLHQDLQTFPQPNAAASNVSRPHARYPSPSDSWPSQILASQLKTPEQHELGSGTSSGTSSRGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z7X5</accession>
    <name>A0AA38Z7X5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_019292</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z7X5"/>
    <dbReference type="SMR" id="A0AA38Z7X5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040008">
        <property type="term" value="P:regulation of growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11442">
        <property type="entry name" value="bHLH_AtPRE_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000082">
        <property type="entry name" value="Transcription factor ILI6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044293">
        <property type="entry name" value="PRE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46446">
        <property type="entry name" value="TRANSCRIPTION FACTOR PRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46446:SF28">
        <property type="entry name" value="TRANSCRIPTION FACTOR PRE5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23174">
        <property type="entry name" value="bHLH_ILI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0341">Growth regulation</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="6">
        <location>
            <begin position="3"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9683653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="90" mass="10240" checksum="59CD9137799B61E5" modified="2024-01-24" version="1">MSSRRSRQSGSSRISDDQIIELVSKLQQLLPEIRNRRSDKVSASKVLQETCNYIRSLHREVDDLSERLSRLLATVDADSPEAAIIRSLIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z801</accession>
    <name>A0AA38Z801_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_016547</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z801"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000036">
        <property type="entry name" value="Alpha/beta-Hydrolases superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051044">
        <property type="entry name" value="MAG_DAG_Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11614">
        <property type="entry name" value="PHOSPHOLIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="1">
        <location>
            <begin position="34"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9684105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="322" mass="36147" checksum="DC964AAB1C364DCF" modified="2024-01-24" version="1">MAGEMRNHVKYEEEFILNSRGLKLFTCRWFPANQDPKALIFILHGYAMECSIFMNDTGTRLAKAGYAVYGIDFEGHGKSSGLGGLISCFDDVVSDCSNYFSTICEHKDNIGKMRYLYGESMGGAIALNLDRQTPDYWDGAVLVAPMCKIADDMRPNPVVITVLTMLCKVIPTWKMIPVEDVVEMAFKEPEKRAEIRSNPYCYKGRIRLKTGQELLRVSLDLEKNLHKIQMPFLVVHGGDDKVTDPSTSKQLHETAASSDKTFKLYPGMWHGLTSGEPLENIDTVFSDIINWLDARSAIGNSRLEKERKSGNDGFISSKNMVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z955</accession>
    <name>A0AA38Z955_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">PVL29_016680</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="25">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20 27">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19 27">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the leguminous lectin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="25">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z955"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002229">
        <property type="term" value="P:defense response to oomycetes"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06899">
        <property type="entry name" value="lectin_legume_LecRK_Arcelin_ConA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000423">
        <property type="entry name" value="L-type lectin-domain containing receptor kinase S.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000108">
        <property type="entry name" value="L-type lectin-domain containing receptor kinase S.4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000086">
        <property type="entry name" value="L-type lectin-domain containing receptor kinase S.4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050528">
        <property type="entry name" value="L-type_Lectin-RKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001220">
        <property type="entry name" value="Legume_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27007">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00139">
        <property type="entry name" value="Lectin_legB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0430">Lectin</keyword>
    <keyword evidence="16 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10 23" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 22" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="23">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041416478" description="non-specific serine/threonine protein kinase" evidence="23">
        <location>
            <begin position="22"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="286"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="24">
        <location>
            <begin position="342"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="371"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="KAJ9684313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="675" mass="75153" checksum="DA69CFAEAFC6F268" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZA67</accession>
    <name>A0AA38ZA67_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_017241</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the mTERF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZA67"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.70.10:FF:000001">
        <property type="entry name" value="Mitochondrial transcription termination factor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.70.10">
        <property type="entry name" value="Transcription termination factor 3, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003690">
        <property type="entry name" value="MTERF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038538">
        <property type="entry name" value="MTERF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13068">
        <property type="entry name" value="CGI-12 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13068:SF236">
        <property type="entry name" value="OS02G0749800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02536">
        <property type="entry name" value="mTERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00733">
        <property type="entry name" value="Mterf"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0806">Transcription termination</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9685129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="369" mass="41799" checksum="23B597BA398CA4D4" modified="2024-01-24" version="1">MFRSLLNSNSNLLRIFSFRPLSSATATATATSTVDYLTNTLCFPRESAIAVAQKLKLKTTTRPDSVVQLFKSYGFAPTHIATIVSKLPSLLLANPVQTLAPKLQFLSNNGVSGSSLVHIVSTNPVILRRSLQKQIIPCISFLKKVLPTDHKIASLLTAKRGTWVVYKFSEQMVPNIETLRSHGVPESNILRMLILRPRTLSFNADEFKAILKRVKEMGFDEKGMMFIHGMCALCGMKKAKWESKVSVFRSFGWGEEEFIALFVKQPQFMSNSETRIRKCLDFLINELDWMPEDIFKYPMVLLLSLEKRVVPRSRVLQLLIGKGLVSRRSIGRALIISEDQFMKVFMSSYEKKIPELLEVYQSNKVGLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZB65</accession>
    <name>A0AA38ZB65_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_017404</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZB65"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF78">
        <property type="entry name" value="OS12G0587133 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="331"/>
            <end position="609"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9685362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1070" mass="123040" checksum="80DFE78FDE5A1FD9" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZBV9</accession>
    <name>A0AA38ZBV9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Senescence-specific cysteine protease SAG39-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_015182</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZBV9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02248">
        <property type="entry name" value="Peptidase_C1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000306">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000515">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025661">
        <property type="entry name" value="Pept_asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025660">
        <property type="entry name" value="Pept_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039417">
        <property type="entry name" value="Peptidase_C1A_papain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00705">
        <property type="entry name" value="PAPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00640">
        <property type="entry name" value="THIOL_PROTEASE_ASN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00639">
        <property type="entry name" value="THIOL_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041384434" description="Senescence-specific cysteine protease SAG39-like" evidence="6">
        <location>
            <begin position="28"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="8">
        <location>
            <begin position="39"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="7">
        <location>
            <begin position="123"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9686163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="341" mass="37379" checksum="5DE7FC044FC81452" modified="2024-01-24" version="1">MASVNQYQYICLALLFVLAAWASQATARNLHEASMYERHEDWMVQYGREYKDADEKSKRYKIFKDNVALIESFNKAVDKSYKLSINEFADLTNEEFRASRNRFKAHICSTEATSFKYENVTAVPSTVDWRKKGAVTPIKDQGQCGSCWAFSAVAAMEGITQLSTGQLISLSEQELVDCDTSGEDQGCSGGLMDDAFKFIEQNHGLTTEASYPYAGTDGTCNSKKAAHPAAKINGYEDVPANNEKALQKAVAHQPIAVAIDAGGAEFQFYSSGVFTGQCGTELDHGVAAVGYGTSDDGMKYWLVKNSWGTAWGEEGYIRMQRDVTAKEGLCGIAMQASYPTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZCP9</accession>
    <name>A0AA38ZCP9_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_015401</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZCP9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9686477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="95" mass="9150" checksum="6D29B6914BC7DD1B" modified="2024-01-24" version="1">MKGGTICFCIPAGRKDKHEDESSSGKSSGRKSHKKAKKRGNAHVGGDDEEGHADSGHGGAANSSAADAGMAVAAISVAQMSSGDGSGHGGGGDGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZCT6</accession>
    <name>A0AA38ZCT6_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_015499</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZCT6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35292">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35292:SF3">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9686655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="100" mass="11044" checksum="A3B835E54575D83B" modified="2024-01-24" version="1">MASRVAAAARQAAILTRLSSAKPSPQSANLIQRRGLAGAADHHGPPRVNVWEDPMSPSKWKEEHFVIVSLSGWGLLFFGSYKFFTRGKKDKEEKLVEASH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZDR7</accession>
    <name>A0AA38ZDR7_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_015520</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HD-ZIP homeobox family. Class IV subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZDR7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08875">
        <property type="entry name" value="START_ArGLABRA2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.530.20:FF:000026">
        <property type="entry name" value="Homeobox-leucine zipper protein GLABRA 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000229">
        <property type="entry name" value="Homeobox-leucine zipper protein HDG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042160">
        <property type="entry name" value="HD-Zip_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057993">
        <property type="entry name" value="HD-Zip_IV_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002913">
        <property type="entry name" value="START_lipid-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45654:SF93">
        <property type="entry name" value="HOMEOBOX-LEUCINE ZIPPER PROTEIN HDG2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45654">
        <property type="entry name" value="HOMEOBOX-LEUCINE ZIPPER PROTEIN MERISTEM L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25797">
        <property type="entry name" value="PDF2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01852">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00234">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50848">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 9 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="8 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="87"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="START" evidence="13">
        <location>
            <begin position="270"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="89"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="64"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="64"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="87"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9686712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="758" mass="82802" checksum="A8E10F6B40E0170D" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZE86</accession>
    <name>A0AA38ZE86_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_016041</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZE86"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9687391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="128" mass="14510" checksum="7DFF00919DE9D4CB" modified="2024-01-24" version="1">MPFMSTAHFTISHHPSYLFADSPCTSFLTFMLPMSILSPKQLSYPDKPIIIPTAHALKPFYHPSNSLLSLSSYPLPNSFTTFPHLFCHAILAHLFSITHKPKFPCKPPTHPPSFIPVFPHHHIHHPSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZEE3</accession>
    <name>A0AA38ZEE3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">PVL29_016111</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="23">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18 25">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17 25">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZEE3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000445">
        <property type="entry name" value="MDIS1-interacting receptor like kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001158">
        <property type="entry name" value="Os01g0296000 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000851">
        <property type="entry name" value="Probable leucine-rich repeat receptor-like protein kinase At1g35710"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001375">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:002048">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051716">
        <property type="entry name" value="Plant_RL_S/T_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48053">
        <property type="entry name" value="LEUCINE RICH REPEAT FAMILY PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48053:SF168">
        <property type="entry name" value="LRR RECEPTOR-LIKE KINASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="14 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="797"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1007"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="1007"/>
            <end position="1021"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="826"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAJ9687495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1104" mass="121435" checksum="F5A4CBFEEB4CD5BA" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZEZ9</accession>
    <name>A0AA38ZEZ9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Anaphase-promoting complex subunit 13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_015835</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the APC13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZEZ9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070979">
        <property type="term" value="P:protein K11-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008401">
        <property type="entry name" value="Apc13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28672">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28672:SF1">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="36"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9687134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="63" mass="7031" checksum="A05F237824F372DB" modified="2024-01-24" version="1">MAELSLGVLIDIVDEEWMRDTLPVDDLPLPPVLVARTEDTEDSNQENQQVDGDTWHDLALGAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZF51</accession>
    <name>A0AA38ZF51_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein BYPASS-related</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_015887</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ROH1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZF51"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008511">
        <property type="entry name" value="ROH1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31509">
        <property type="entry name" value="BPS1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05633">
        <property type="entry name" value="ROH1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="242"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="182"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9687209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="392" mass="44779" checksum="A021CAA85486C78A" modified="2024-01-24" version="1">MPSTENQGSSSPFMSFGRSILSIRREQVHSMEANHDPNTQESEIELFLQLISKRFHDLSVVSADELLSLAWMRKLLDAFICCHEEFRVILLNNKVQVCKPPLERLVAEFFDRCLKALDICNATRDGIEKIRQWEKQLEIVLSAMDSRQRTMGEGQFRRARKALMDLAIAMLDEKETGSIFSHRNRSFGRHQSSKDQRHHLPGHSRSLSWSVSRSWSAAKQLQSIANNLVPPRGNDISATNGLAVPVFAMSYVLMFVLWALVAAIPCQDRGLQIHCSVPRHFSWGAPFILLQERIMEESKKRERRNSSGLLKEINQMERCASHMTDLVDSIHFPLTDELKTEAGQQVQEVTLLCEALKNGLDPLERQVREVFRKIMNGRTEGLEFLGSVNKPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZFL2</accession>
    <name>A0AA38ZFL2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_016129</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AAA ATPase family. BCS1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZFL2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19510">
        <property type="entry name" value="RecA-like_BCS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001122">
        <property type="entry name" value="AAA-ATPase ASD, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025753">
        <property type="entry name" value="AAA_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058017">
        <property type="entry name" value="At3g28540-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050747">
        <property type="entry name" value="Mitochondrial_chaperone_BCS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23070">
        <property type="entry name" value="BCS1 AAA-TYPE ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14363">
        <property type="entry name" value="AAA_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25568">
        <property type="entry name" value="AAA_lid_At3g28540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="12">
        <location>
            <begin position="230"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="302"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="434"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="316"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9687523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="473" mass="54569" checksum="B0B3ECB856031329" modified="2024-01-24" version="1">MGEMLGNLGSVMAALMFIWAMFQQYFPHDLRRHIEKYSHRLMKFFYPYIQITVPEYGRDHFMRNEVYTAIETYLSSNTAVQAKRLKADTAKNNQSLVLTIDDHEEVEDEFEGVRLWWASSTIIARNQTFPFYGQPDEKRYYRLTFHKKHRDLITKEYLSHVLREGKAINVRTRQRKLYTNNGSMWSHVVFDHPATFHTLAMEAEKKREMIEDLVSFSKAEDFYARIGKAWKRGYLLYGPPGTGKSTMIAAMANLLEYDVYDLELTAVKDNTELRKLLIQTSSKSIIVIEDIDCSLDLTGQRKKKKENEAAEEEEKDPIKKQAKVGDSDQGKTSKVTLSGLLNFIDGLWSACKGERLIVFTTNYMEKLDPALIRRGRMDKHIELSYCSFESFKVLARNYLELDSHHLFDTIEGLLGESRVTPADVAEHLMPKTSVADAETSLKSLVQALEMAKEEAMLKAKEEAKEESSARKED</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZFV8</accession>
    <name>A0AA38ZFV8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Probable magnesium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_016158</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 8">Acts as a Mg(2+) transporter. Can also transport other divalent cations such as Fe(2+), Sr(2+), Ba(2+), Mn(2+) and Co(2+) but to a much less extent than Mg(2+).</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Early endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the NIPA (TC 2.A.7) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZFV8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008521">
        <property type="entry name" value="Mg_trans_NIPA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12570:SF89">
        <property type="entry name" value="MAGNESIUM TRANSPORTER NIPA3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05653">
        <property type="entry name" value="Mg_trans_NIPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 8" id="KW-0967">Endosome</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="86"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="119"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="186"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="227"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="251"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="287"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="317"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9687554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="350" mass="38114" checksum="46C829CBBB55658B" modified="2024-01-24" version="1">MAAAGEEGAYSYKGMSADNIKGLVLALSSSFFIGASFIVKKKGLKKAGASGIRAGVGGYSYLYEPLWWVGMITMIVGEIANFAAYAFAPAILVTPLGALSIIISAVLAHIILREKLHIFGILGCVLCVVGSTTIVLHAPQEREIQSVIEVWDLATEPAFLFYAALVITAVFVLIIHFIPQYGQTHIMVYIGVCSLVGSLSVMSVKAIGIALKLTLSGMNQLTYPQTWAFTIVVITCVITQMNYLNKALDTFNTAVVSPIYYVMFTSLTILASVIMFKDWDRQNPTQIVTEMCGFVTILSGTFLLHKTKDLSDGMLSTLIVIFILLMLLLGHSPGTCCSWILYFIFVKKEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZHV7</accession>
    <name>A0AA38ZHV7_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_014726</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9689207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZHV7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9689207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="260" mass="28519" checksum="EB6FABB3334B6E49" modified="2024-01-24" version="1">MEFIVGANLHWFTLDDTAFADSLSLMNSLTKTKGPNLIAKLIGLEESPSIPFQTTSHKYLEGGTTMNQKKPLFGIDILKNAQTPQHNWLKAQFNFWTKLSKSMKTRSSSRNLIVKRASKQFSVTFYAVSVDSSTSNTLSSAPSALSGEHASVVINNEGSSKRNLETPLLAPQELNDQNSQKGGNESLVRPSLNISLNSSLGPPTFLLHFPGHAVEGIEWSKSAFTFQRGQGHPQDYFGDGYRATAPRLAYGQPQPIPGYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZIQ9</accession>
    <name>A0AA38ZIQ9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PGG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_012473</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9689820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZIQ9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026961">
        <property type="entry name" value="PGG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24177">
        <property type="entry name" value="CASKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24177:SF314">
        <property type="entry name" value="PROTEIN ACCELERATED CELL DEATH 6-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13962">
        <property type="entry name" value="PGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="364"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="404"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="450"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="484"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="domain" description="PGG" evidence="3">
        <location>
            <begin position="360"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9689820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="573" mass="65269" checksum="7C88445CC66AE131" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZJ92</accession>
    <name>A0AA38ZJ92_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Gnk2-homologous domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_012643</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZJ92"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23509">
        <property type="entry name" value="Gnk2-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.430.20:FF:000002">
        <property type="entry name" value="Cysteine-rich receptor-like protein kinase 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.430.20">
        <property type="entry name" value="Gnk2 domain, C-X8-C-X2-C motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002902">
        <property type="entry name" value="GNK2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038408">
        <property type="entry name" value="GNK2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32099:SF51">
        <property type="entry name" value="CYSTEINE-RICH RECEPTOR-LIKE PROTEIN KINASE 25 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32099">
        <property type="entry name" value="CYSTEINE-RICH REPEAT SECRETORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01657">
        <property type="entry name" value="Stress-antifung"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51473">
        <property type="entry name" value="GNK2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041424068" description="Gnk2-homologous domain-containing protein" evidence="4">
        <location>
            <begin position="24"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="5">
        <location>
            <begin position="26"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="5">
        <location>
            <begin position="130"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="243"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9690090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="282" mass="31116" checksum="B7A4F4E66814490E" modified="2024-01-24" version="1">MGCLGLVVFISSIQMHLLTIALGQNFLRQFCMRERGNYTNNSAYQANLDSLLSAFCNTTVDYGFYNSSAGEVKAIALCRGDLTPNTCRKCIGTSSHELRGLCPNYKEAYIYYDNCMLGYSNRSIFSINKLSPIYISAKLENFSDVNQTNQVLPKLLGNLKDEAASGSPLRKYAVGEATVGFETIYALVQCTPDLQKQQCTNCLDDLNVLIPKNFSEGKNGLTAIGPICNFRYEVYRFYQSRPDAPSSFPSPTGDAPSPAASNDTTGNGMQLNLHACFEYCRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZKZ7</accession>
    <name>A0AA38ZKZ7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SBP-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_012910</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZKZ7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1100.10">
        <property type="entry name" value="Transcription factor, SBP-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044817">
        <property type="entry name" value="SBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004333">
        <property type="entry name" value="SBP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036893">
        <property type="entry name" value="SBP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31251:SF197">
        <property type="entry name" value="SQUAMOSA PROMOTER-BINDING-LIKE PROTEIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31251">
        <property type="entry name" value="SQUAMOSA PROMOTER-BINDING-LIKE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03110">
        <property type="entry name" value="SBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103612">
        <property type="entry name" value="SBT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51141">
        <property type="entry name" value="ZF_SBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SBP-type" evidence="6">
        <location>
            <begin position="53"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="107"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="107"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="118"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9690492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="128" mass="14233" checksum="490E41D263F5B72C" modified="2024-01-24" version="1">MEMGKAQGKRTLMEEEEEDDEDTVGLGFGEDEKKKKAVTLTNKKGSGGAGSTPPCCQVDNCTVDMSEAKRYHKRHRVCEHHAKAPVILIAGIQQRFCQQCSRSCRRRLAGHNERRRKSSSESHGEGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZLC5</accession>
    <name>A0AA38ZLC5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">alpha-1,2-Mannosidase</fullName>
            <ecNumber evidence="13">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_013237</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N(4)-(alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 9A1,2,3B1,2,3) + 4 H2O = N(4)-(alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2) + 4 beta-D-mannose</text>
            <dbReference type="Rhea" id="RHEA:56008"/>
            <dbReference type="Rhea" id="RHEA-COMP:14356"/>
            <dbReference type="Rhea" id="RHEA-COMP:14367"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28563"/>
            <dbReference type="ChEBI" id="CHEBI:59087"/>
            <dbReference type="ChEBI" id="CHEBI:139493"/>
            <dbReference type="EC" id="3.2.1.113"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>N(4)-(alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 8A1,2,3B1,3) + 3 H2O = N(4)-(alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2) + 3 beta-D-mannose</text>
            <dbReference type="Rhea" id="RHEA:56028"/>
            <dbReference type="Rhea" id="RHEA-COMP:14358"/>
            <dbReference type="Rhea" id="RHEA-COMP:14367"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28563"/>
            <dbReference type="ChEBI" id="CHEBI:59087"/>
            <dbReference type="ChEBI" id="CHEBI:60628"/>
            <dbReference type="EC" id="3.2.1.113"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the glycosyl hydrolase 47 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="13"/>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZLC5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004571">
        <property type="term" value="F:mannosyl-oligosaccharide 1,2-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000025">
        <property type="entry name" value="alpha-1,2-Mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001382">
        <property type="entry name" value="Glyco_hydro_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050749">
        <property type="entry name" value="Glycosyl_Hydrolase_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036026">
        <property type="entry name" value="Seven-hairpin_glycosidases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11742:SF55">
        <property type="entry name" value="ENDOPLASMIC RETICULUM MANNOSYL-OLIGOSACCHARIDE 1,2-ALPHA-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11742">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE ALPHA-1,2-MANNOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01532">
        <property type="entry name" value="Glyco_hydro_47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00747">
        <property type="entry name" value="GLYHDRLASE47"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48225">
        <property type="entry name" value="Seven-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0106">Calcium</keyword>
    <keyword evidence="7 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="226"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="370"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="498"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="539"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="626"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="441"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9690979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="640" mass="71657" checksum="DA5FC36AB2FCD2FB" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZP40</accession>
    <name>A0AA38ZP40_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phytocyanin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_011663</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZP40"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13920">
        <property type="entry name" value="Stellacyanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000034">
        <property type="entry name" value="Cupredoxin superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028871">
        <property type="entry name" value="BlueCu_1_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039391">
        <property type="entry name" value="Phytocyanin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003245">
        <property type="entry name" value="Phytocyanin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33021">
        <property type="entry name" value="BLUE COPPER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33021:SF189">
        <property type="entry name" value="CUCUMBER PEELING CUPREDOXIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02298">
        <property type="entry name" value="Cu_bind_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00196">
        <property type="entry name" value="COPPER_BLUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51485">
        <property type="entry name" value="PHYTOCYANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0186">Copper</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041384143" description="Phytocyanin domain-containing protein" evidence="6">
        <location>
            <begin position="26"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="Phytocyanin" evidence="7">
        <location>
            <begin position="27"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="131"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="135"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="149"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="185"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="199"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="231"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="266"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9692685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="299" mass="30140" checksum="5B9F59D017DBC47E" modified="2024-01-24" version="1">MARFMCMVGVMIVVAATLMVNFAAAQTVHVVGDSLGWTVPPNGAAAYTSWASNKQFMVGDILVFNFATNEHDVVELSKESFDACNYSNPIGSIITTGPANITLNATGNHYYICTIGRHCAAGQKLAITVSATPSSNPPSASPASPPPTTIATPSPTATPDDCAPTPSPSLMTDAPPAATPTQTPATPPPTATKPPTGTPSPTATPDDCAPTPSPSLMTDAPPVATPTQTPTAPPPTATKPPPGTPSPTGNPDDCAPTPSPNPMTGSPAPATPSLDDSSSSAVFASALAFLVSVAMSFFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZP64</accession>
    <name>A0AA38ZP64_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">RecA family profile 1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_011335</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZP64"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000725">
        <property type="term" value="P:recombinational repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01121">
        <property type="entry name" value="RadA_SMS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000050">
        <property type="entry name" value="DNA repair protein RadA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000053">
        <property type="entry name" value="DNA repair protein radA isogeny"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004504">
        <property type="entry name" value="DNA_repair_RadA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00416">
        <property type="entry name" value="sms"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32472">
        <property type="entry name" value="DNA REPAIR PROTEIN RADA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32472:SF10">
        <property type="entry name" value="DNA REPAIR PROTEIN RADA-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13481">
        <property type="entry name" value="AAA_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13541">
        <property type="entry name" value="ChlI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01874">
        <property type="entry name" value="DNAREPAIRADA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="RecA family profile 1" evidence="10">
        <location>
            <begin position="233"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="54"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="65"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="88"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="99"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9692217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="629" mass="68003" checksum="FB39F68A3A3ABE58" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZQ16</accession>
    <name>A0AA38ZQ16_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NB-ARC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_012072</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9693171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZQ16"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.430:FF:000003">
        <property type="entry name" value="Probable disease resistance protein At5g66910"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050905">
        <property type="entry name" value="Plant_NBS-LRR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463:SF220">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0611">Plant defense</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NB-ARC" evidence="5">
        <location>
            <begin position="156"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="25"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9693171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="377" mass="43034" checksum="55DAA1E5FB66357C" modified="2024-01-24" version="1">MEFLSSILGLVPCLYDQTSKHTVYIRDLKKNLQALRKEMVGLNNLYEDVKERVEGAEQRQMRRRKEVGGWICEVEDMLKEVNEILRRGDQEIQKRCLGCCPRNCWSSYRIGKAVSEKLVALSDQISKGYFDAVADMPPRPPVDELPMKEIVGSELTYNRICGFLKDPQVGIMGLYGMGGVGKTTLLKKINNDFLNTSSHFDVVIWVVVSKPSNIEKIQEVIWNKLQIPRDIWEIRNSKEEKAAEILRVLKTKKFVLLLDDIWERLDLLEMGVPHPDAQNKSKIVFTTRSQDVCHQMKAQKSIEVACLSSEATWTLFQKEVGEETLKSHPHIPRLAKIVAEECKGLPLALITLGRAMAGEKDPTNWDKVLQDLRQISS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZQT2</accession>
    <name>A0AA38ZQT2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glycerol kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_011984</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FGGY kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9693072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZQT2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004370">
        <property type="term" value="F:glycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006071">
        <property type="term" value="P:glycerol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046167">
        <property type="term" value="P:glycerol-3-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006641">
        <property type="term" value="P:triglyceride metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10196:SF69">
        <property type="entry name" value="GLYCEROL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10196">
        <property type="entry name" value="SUGAR KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9693072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="121" mass="13178" checksum="6ED4884D35BDBCE2" modified="2024-01-24" version="1">MIGGLHVGVHVTDVSNASGTMLMNLKTLDWDKPTSDTNYVLEGSIVIASEIEELVAKVNDVLDSMHKDAGEKGEVKNDKVEFLLRVDGGATINNLLMQMQANLLGNPVFRPTTIEPLMQLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZR75</accession>
    <name>A0AA38ZR75_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Shikimate O-hydroxycinnamoyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_011845</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZR75"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050317">
        <property type="entry name" value="Plant_Fungal_Acyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31642:SF266">
        <property type="entry name" value="HXXXD-TYPE ACYL-TRANSFERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31642">
        <property type="entry name" value="TRICHOTHECENE 3-O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9692923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="467" mass="51192" checksum="591E447C4B9AAB43" modified="2024-01-24" version="1">MGGGGDFTVTLTKKEVVEAMLPMQEHWLPLSNLDLLLPPVDVSVFFCYKNPHDQSVNGGHVFSFGSMVGALKIAMAQALVSYYAFAGEVVSNSAGEPELLCNNRGVDFMEAHADVRLENLNLYNPDASIEGKLVPKKENGVLSVQVTELKCGGVVVACTFDHRIADAYSANMFLVSWAEMAQSKPLSVTPSFQRSLLSTRRPGSYDPSFDDMYVPISALPPPKAPQPSADPLMSRLYYVTAEQLSLLQMLATSKSSSCKRTKLESLSAFLWKMVAKSADVDDDNKKICKMGIVVDGRRRLSSGDEDKAAAMANYFGNVLSIPFGEKIIDELKEKPLSWVGDAVHEYLEGAVTREHFLGLIDWVEAHRPEPAVAKIYCSGSNDGPAFVVSSGQRFPASKVNFGWGRPALGSYHFPWGGEAGYVMPMPSPVRDGDWVVYVHLLRGQIELIETEAAHVFRPLTPEYLKLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZT74</accession>
    <name>A0AA38ZT74_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">3-oxoacyl-[acyl-carrier-protein] reductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_010039</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZT74"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44375">
        <property type="entry name" value="BETA-KETOACYL-ACP REDUCTASE-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44375:SF2">
        <property type="entry name" value="BETA-KETOACYL-ACP REDUCTASE-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9694347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="286" mass="30340" checksum="8E3B7EE92AC1490E" modified="2024-01-24" version="1">MACKVENHLQPWQDLEGKVVMVTGASSGLGSEFCLNLAKAGCKIVAAARRVDRLKSLCDEINNLTHSNLPPNADPPLRAVAVELDITSDGSSIGASVQKAWEAFGRIDALVNNAGIRGNMNSLLDISEEEWNNTLKTNLTGSWLVSKYVGMRMRDAKNGGSIINISSIAGLNRGQLPGGVAYVSSKSGLGAMTKVMALELGAYNIRANAIAPGIFKSEITAGLMQKDWLNTVVQKTCPLRTYGTSDPALTSLVRYLIHDSSGYISGNIFIVDAGATLPGVPIFSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZUA1</accession>
    <name>A0AA38ZUA1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">FAS1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_010123</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">May be a cell surface adhesion protein.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the fasciclin-like AGP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZUA1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009834">
        <property type="term" value="P:plant-type secondary cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.180.10:FF:000006">
        <property type="entry name" value="Fasciclin-like arabinogalactan protein 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.180.10">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036378">
        <property type="entry name" value="FAS1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000782">
        <property type="entry name" value="FAS1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045003">
        <property type="entry name" value="FLA_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32077">
        <property type="entry name" value="FASCICLIN-LIKE ARABINOGALACTAN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32077:SF65">
        <property type="entry name" value="FASCICLIN-LIKE ARABINOGALACTAN PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02469">
        <property type="entry name" value="Fasciclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00554">
        <property type="entry name" value="FAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82153">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50213">
        <property type="entry name" value="FAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="4" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0654">Proteoglycan</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041332182" description="FAS1 domain-containing protein" evidence="11">
        <location>
            <begin position="25"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="12">
        <location>
            <begin position="83"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="26"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="235"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="257"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9694483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="294" mass="29861" checksum="4B0F6780B4C5CCB7" modified="2024-01-24" version="1">MMKQLLSPTLLLLIFFLLCTTTSGQSSSPAAAPSGPTTPSGQSSPPAAAPSGPTTTSGQSSPPALAPSVPLVPSGPSGTPSGSLDITAILRKARKFTTFIGLLKSTQMDAEINTRLKKSDGITVFAPADSAFSNLKTGTLNSFTDRQKTALARFHIVLSFHTIPQFQTVSNPVHTAADGDTVAFPLNVIGDGKQVNMTTGLVNTTVDSTVYSDGQLAVYEIGQVLLSDGVLGGQAPAPAPLPPKPKKASPPTSHAPTRSTTVSVDSSGATGPPHGATIVVSIGVAVLAALPLCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZUE5</accession>
    <name>A0AA38ZUE5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Inositol polyphosphate-related phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_010161</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the inositol polyphosphate 5-phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZUE5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004445">
        <property type="term" value="F:inositol-polyphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034485">
        <property type="term" value="F:phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004439">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045849">
        <property type="entry name" value="IP5P_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000300">
        <property type="entry name" value="IPPc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45666:SF15">
        <property type="entry name" value="TYPE I INOSITOL POLYPHOSPHATE 5-PHOSPHATASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45666">
        <property type="entry name" value="TYPE IV INOSITOL POLYPHOSPHATE 5-PHOSPHATASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22669">
        <property type="entry name" value="Exo_endo_phos2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00128">
        <property type="entry name" value="IPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inositol polyphosphate-related phosphatase" evidence="3">
        <location>
            <begin position="91"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9694543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="524" mass="59357" checksum="93A557A87617503F" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZVP8</accession>
    <name>A0AA38ZVP8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">WD repeat-containing protein 4 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_008419</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Required for the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA. In the complex, it is required to stabilize and induce conformational changes of the catalytic subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">tRNA modification; N(7)-methylguanine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Forms a heterodimer with the catalytic subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the WD repeat TRM82 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZVP8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043527">
        <property type="term" value="C:tRNA methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106004">
        <property type="term" value="P:tRNA (guanine-N7)-methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001042">
        <property type="entry name" value="tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001350">
        <property type="entry name" value="tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03056">
        <property type="entry name" value="TRM82"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028884">
        <property type="entry name" value="Trm82"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16288:SF0">
        <property type="entry name" value="TRNA (GUANINE-N(7)-)-METHYLTRANSFERASE NON-CATALYTIC SUBUNIT WDR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16288">
        <property type="entry name" value="WD40 REPEAT PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 6" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="2 6 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="61"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="198"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9696165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="433" mass="46968" checksum="4EFE2D3904D2BEA8" modified="2024-01-24" version="1">MEEPAIEEGDNHRDVEVAPALISVHPDHKFIVVSIGSDLRVFDLQGGCSVTLVDDSSGPLHKDSIRAIRFGAEGKLFVSAGDDKLVKIWNANSWHCISTVCSEKRVSAVAISKDGLFVCFADKFGVVWALNLDGFDENQVLVEKKAAPVLAHYCSIITSLEFSLDGRFIISADRDFKIRVSVFPKKPLDGAHEIQSFCLGHTEFVSCLAFVCAPDYPEGFLVSGSGDSTVRLWDITSGSLLDTCEVGSQAGFLESNGREEEHYSAVIDLCTTPDGTLVAVAIQSLQGILLLNCDLSAKSLSIAKVVSITGGSFIPTSLGTASCAEFLWMVTGVSTLHGFDSHSLARVRVISGFRIGSPDSVEHEPTMLEDDEIPGGEKLLEKLQGSVSIENKVFSAAAEAVKMAMCNLLIKKQYSADKREFRKRTRNDKKIKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZW84</accession>
    <name>A0AA38ZW84_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Patatin</fullName>
            <ecNumber evidence="6">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_008444</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Lipolytic acyl hydrolase (LAH).</text>
    </comment>
    <comment type="domain">
        <text evidence="6">The nitrogen atoms of the two glycine residues in the GGXR motif define the oxyanion hole, and stabilize the oxyanion that forms during the nucleophilic attack by the catalytic serine during substrate cleavage.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the patatin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="6"/>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZW84"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07199">
        <property type="entry name" value="Pat17_PNPLA8_PNPLA9_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32241:SF13">
        <property type="entry name" value="INACTIVE PATATIN-LIKE PROTEIN 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32241">
        <property type="entry name" value="PATATIN-LIKE PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 6" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="4 6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNPLA" evidence="7">
        <location>
            <begin position="31"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DGA/G" evidence="5">
        <location>
            <begin position="219"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9696199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="337" mass="37257" checksum="81E77DCF669A7B7F" modified="2024-01-24" version="1">MELSKVTLEIFSKLEQKWLSHCEGSKKTRVLSIDGGGTTAVVSAAALVHLEDQIQLKTGDSHSRIVDFFDVVVGTGIGAVFAAMLTADDGSGRPLFSAKEAVRFLTEKQCEMFKIKHVGVFRRRRRFSGRSMDKVLKEALRREDGKVLTLKDTCKPLLVPCFDLNSSAPFVFSRADASESPSFDFELWKVCRATTATPSMFKPFALTSVDGKTSCSAVDGGLVMNNPTAAAVTHVLHNKRDFPSVNGVEDLLVLSLGNGSQTDPSRRRLRHGGECSTSTVVDIVLDGVSETVDQMLGNAFCWNRTDYVRIQVYFISNQICVCFLRKCRKIRTKKKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZWF8</accession>
    <name>A0AA38ZWF8_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_008504</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZWF8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9696304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="163" mass="18324" checksum="0D2C3E096F711E10" modified="2024-01-24" version="1">MQKEEYDSKPLGICHRLFNLVLSRLIGFRPPVSTDVPIEYKNRGDKKRGSPQAQLQKGPETVPVKDQRNELPAVRDIAISNGKTSADQLQDKGPPMVMPNPKKGGKASGTAGKVRLPIEEGNMKERPWPSVTLVTNINEMSDDFIRQRKEAMQRTNTMEPKKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZY22</accession>
    <name>A0AA38ZY22_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_008988</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZY22"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31284:SF57">
        <property type="entry name" value="ACID PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31284">
        <property type="entry name" value="ACID PHOSPHATASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="162"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="190"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9697025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="247" mass="29757" checksum="5519C3E887902327" modified="2024-01-24" version="1">MGKNLGFVLASQASSVSSFEEHFEAWKSSLVRREEGPENYCEVGGSMLIFERAIEESVLYLSKGCCSLKGDGNRRWIFVIDDTLCPLLPYYKKHHFWKLNLTSLEEWMRKHGHKPLGEDRRFFNESGEEASSFKIFLNLPEGNATLSLLHLSKVGYHGWTSIFFLSLFFYYYFFFFFFYFYYIIFFFLFFFYLFSFFYFLFICFLLYFLFFYFYYRIWGIVGDHWSSFEGPPRKRTFKLPIPCICFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZZ01</accession>
    <name>A0AA38ZZ01_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_008870</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZZ01"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052422">
        <property type="entry name" value="Auxin_Ser/Thr_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47986:SF1">
        <property type="entry name" value="OS04G0685900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47986">
        <property type="entry name" value="OSJNBA0070M12.3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9696869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="202" mass="22862" checksum="DE83519DAA15B19C" modified="2024-01-24" version="1">MKETSQRVDSNLWPHVFCSGDRVTQIQVQGLGLKGPLPQNFNQLSMLYNLGFQRNHFNGKLPSFRGLSELQFAFLDYNKFDTIPADFFDGLTSIRILALNDNPFNATTGWSIPDKFVRTTARAEADMPSSSHPARLPTFHASAQEPWHALIAYDACVRLCLHAWAGYMDAPMFLESECALLRNAFGLQQVLLQSEENYPLKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A0T2</accession>
    <name>A0AA39A0T2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DC1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_007120</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9697843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A0T2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004146">
        <property type="entry name" value="DC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46288:SF17">
        <property type="entry name" value="CYSTEINE_HISTIDINE-RICH C1 DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46288">
        <property type="entry name" value="PHORBOL-ESTER/DAG-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03107">
        <property type="entry name" value="C1_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="DC1" evidence="2">
        <location>
            <begin position="19"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="DC1" evidence="2">
        <location>
            <begin position="132"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9697843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="196" mass="22206" checksum="036ED32434788F26" modified="2024-01-24" version="1">MSRHNNEEQEEERLLLRHFSHEHPLELACDDSSRPEADRVTCVGCGIHLLPRKAYYTCRTCDFSLHRPCYNMPRKVHHPTDPGHDLVLHLSTSFACKGCGNPGSGFSYHCGICLQSYHILCSVLPLSISHYSHPHVLKLEFSPPNYDGLEGFCCDICKNPGSHHWLYRCGTCEFDVHLHCAISNGQGHQSHTQETN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A135</accession>
    <name>A0AA39A135_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">26S proteasome regulatory subunit 6B homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_007803</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A135"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19502">
        <property type="entry name" value="RecA-like_PAN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000018">
        <property type="entry name" value="26S protease regulatory subunit 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000046">
        <property type="entry name" value="26S protease regulatory subunit 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000033">
        <property type="entry name" value="26S protease regulatory subunit 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050221">
        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032501">
        <property type="entry name" value="Prot_ATP_ID_OB_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23073">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16450">
        <property type="entry name" value="Prot_ATP_ID_OB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0647">Proteasome</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="14">
        <location>
            <begin position="198"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="58"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="18"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00068703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9698912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="418" mass="47087" checksum="61878A02594E9AD9" modified="2024-01-24" version="1">MAMVLEPKPSSEPPPSFPSTRSDIQGFSDQSSSFSDEDDLYGRLKSLQRQLEFIDIQEEYVKDEQKNLKRELLRAQEEVKRIQSVPLVIGQFMEMVDQNNGIVGSTTGSNYYVRILSTINRELLKPSASVALHRHSNALVDVLPPEADSSISLLSQSEKPDVTYNDIGGCDIQKQEIREAVELPLTHHELYKQIGIDPPRGVLLYGPPGTGKTMLAKAVANHTTAAFIRVVGSEFVQKYLGEGPRMVRDVFRLAKENAPAIIFIDEVDAIATARFDAQTGADREVQRILMELLNQMDGFDQTVNVKVIMATNRADTLDPALLRPGRLDRKIEFPLPDRRQKRLVFQVCTAKMNLSDEVDLEDYVSRPDKISAAEIAAICQEAGMHAVRKNRYVILPKDFEKGYRTNVKKPDTDFEFYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A2C6</accession>
    <name>A0AA39A2C6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Histone deacetylase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_008194</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histone deacetylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9699484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A2C6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004407">
        <property type="term" value="F:histone deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09996">
        <property type="entry name" value="HDAC_classII_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF17">
        <property type="entry name" value="HISTONE DEACETYLASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="8">
        <location>
            <begin position="43"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9699484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="379" mass="40867" checksum="B260F71555FDBB6F" modified="2024-01-24" version="1">MAGLSPSGGINVFWHEGMLKHDTGNGVFDTGMDPGFLDVLEKHPENSERLRNMVSILKRGPISPYISWHSGTPALISHLLSFHTPEYINELVQADRDGGKELCSGTFLNPGSWDAALLAAGTALSAMKHILDGHGKIAYALVRPPGHHAQPSQADGYCFLNNAGLAVQLALNAGCAKVAVIDIDVHYGNGTAEGFYHSDKVLTISLHMNHGSWGPSHPQSGSVDEIGEGEGFGYNLNIPLPNGTGDRGYAYAMNQLVVPAIQKYEPEMMVLVVGQDSSAFDPNGRQCLTTEGYRDIGQIVRKLADKYSDGRLLIVQEGGYQVTYSAYCLHATLEGVLNLPSPLLSDPIAYYPEDELMTTKVIEAIKKFQNSTAPFLKGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A3R6</accession>
    <name>A0AA39A3R6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein GFS12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_005661</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9699912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06071">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1540.10">
        <property type="entry name" value="BEACH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000409">
        <property type="entry name" value="BEACH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036372">
        <property type="entry name" value="BEACH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46866">
        <property type="entry name" value="GH12955P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46866:SF1">
        <property type="entry name" value="GH12955P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02138">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01026">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81837">
        <property type="entry name" value="BEACH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50197">
        <property type="entry name" value="BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="119"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="domain" description="BEACH" evidence="6">
        <location>
            <begin position="355"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1549"/>
            <end position="1584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="667"/>
            <end position="690"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9699912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1677" mass="186031" checksum="DB8D9A3902E35533" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A3Y2</accession>
    <name>A0AA39A3Y2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_008177</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9699463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A3Y2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd19101">
        <property type="entry name" value="AKR_unchar"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43147:SF2">
        <property type="entry name" value="NADP-DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43147">
        <property type="entry name" value="PROTEIN TAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="1">
        <location>
            <begin position="133"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9699463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="457" mass="51147" checksum="D9809991BA95ECDF" modified="2024-01-24" version="1">MAVTVHHVCSHLSSFHPVRTRLTTRPVSRKFGANSVRCDGSIETQRVTVKNGNDSLDICRVLNGMWQTSGGWGRIDRNDAVEAMLRYADAGLSTFDMADHLRKLSSRPFQCVLTEDNRTVLVKNGKDSLDICRIVNGMWQTSGGWGRIVPDDAVDAMLRYADAGFTTFDMADIYGPAEDLYGIFINRVRRERPPEFLDKVRGLTKWVPPPVKMTSKFVGESINVSRKRMDVAALDMLQFHWWDYSNPGYLDALKHLTDLKEEGKIKTVALTNFDTERLQIILENEIPVVSNQVQHSIVDMRPQQKMAELCQLTGVKLITYGTVMGGLLSEKFLDTNIAIPFSAPPLNTPSLQKYKRMVDAWGGWSLFQTLLRTLKTVATKHGVSIPTVAVKYILDQPAVAGSMVGVRLGLSEHINDSNAVFSLVLDEDDVNSIQEVLKKGKDLLRVIGDCGDEYRRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A7X0</accession>
    <name>A0AA39A7X0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transcription factor HY5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_004334</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A7X0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009585">
        <property type="term" value="P:red, far-red light phototransduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010099">
        <property type="term" value="P:regulation of photomorphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010218">
        <property type="term" value="P:response to far red light"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010114">
        <property type="term" value="P:response to red light"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14704">
        <property type="entry name" value="bZIP_HY5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.490:FF:000004">
        <property type="entry name" value="Transcription factor HY5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.490">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044280">
        <property type="entry name" value="Hac1/HY5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46714">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR HAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46714:SF6">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR HAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-0607">Phytochrome signaling pathway</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="BZIP" evidence="12">
        <location>
            <begin position="88"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="29"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="47"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00070194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00084091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9702542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="168" mass="18342" checksum="212682B12B1EB445" modified="2024-01-24" version="1">MQEQATSSLAASSLPSSSERSSSSALQAEIKEGMESDEEIRRVPEIGSGDPAGPSASGREAALVAGPDRVQASGDGQRKRGRSPADKENKRLKRLLRNRVSAQQARERKKAYLNELEVRVKDLERKNSELEERLSTLQNENQMLRHILKNTTASRRGGSSNSNADGSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A8I8</accession>
    <name>A0AA39A8I8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PVL29_003887</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9701863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A8I8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033612">
        <property type="term" value="F:receptor serine/threonine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:002903">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000512">
        <property type="entry name" value="Receptor-like protein kinase HSL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000548">
        <property type="entry name" value="Receptor-like protein kinase HSL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001541">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001675">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050647">
        <property type="entry name" value="Plant_LRR-RLKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056:SF29">
        <property type="entry name" value="RECEPTOR-LIKE PROTEIN KINASE HSL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="16" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041228364" description="Protein kinase domain-containing protein" evidence="16">
        <location>
            <begin position="31"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="635"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="692"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="977"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="721"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ9701863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="997" mass="111085" checksum="E13F3D58BC5DCED5" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ABI8</accession>
    <name>A0AA39ABI8_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_002916</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ARG7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ABI8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009733">
        <property type="term" value="P:response to auxin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003676">
        <property type="entry name" value="SAUR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31175">
        <property type="entry name" value="AUXIN-RESPONSIVE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31175:SF111">
        <property type="entry name" value="AUXIN-RESPONSIVE PROTEIN SAUR68-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02519">
        <property type="entry name" value="Auxin_inducible"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9704583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="148" mass="16940" checksum="39A86DC5BA2CD7F5" modified="2024-01-24" version="1">MISPKKLIRMARKWQKMAAFGRKRISLQRINEGVDEESCSTSSVADKGHFVVYSSDRRRFVIPLVYLDSEIMRELFQMSEEEFGIQSTSPIILPCDSVFLDYVISFIQRGVAKELERALIMSIAPSNCSSSSYFHKEQTNEQLLLCAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ACA2</accession>
    <name>A0AA39ACA2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_003165</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Heterogeneous nuclear ribonucleoprotein (hnRNP)-protein binding the poly(A) tail of mRNA and probably involved in some steps of pre-mRNA maturation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with the poly(A) tail of mRNA in nucleus.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic granule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the polyadenylate-binding RBP47 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ACA2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12344">
        <property type="entry name" value="RRM1_SECp43_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12345">
        <property type="entry name" value="RRM2_SECp43_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12346">
        <property type="entry name" value="RRM3_NGR1_NAM8_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000395">
        <property type="entry name" value="Polyadenylate-binding protein RBP47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000737">
        <property type="entry name" value="polyadenylate-binding protein RBP47 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000144">
        <property type="entry name" value="Polyadenylate-binding protein RBP47B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050825">
        <property type="entry name" value="RBM42_RBP45_47-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47640:SF9">
        <property type="entry name" value="POLYADENYLATE-BINDING PROTEIN RBP47B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47640">
        <property type="entry name" value="TRNA SELENOCYSTEINE 1-ASSOCIATED PROTEIN 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 10" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="81"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="174"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="293"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00057395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00063471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9704988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="414" mass="45953" checksum="786BB5C11A1572FC" modified="2024-01-24" version="1">MQSSNGGDMSQDHQQWMQQQQQQQQQQQQWMALQYPAAAMVMQHQMMYPQHYMPYHHPQPQQQYQNHPPQHQMHGSTDEIKTLWVGDLHQWMDDNYLRSCFGHTGEVSSIKIIRNKQTGQSEGYGFVEFFSRATAEKILHSYNGTLMPNTEQPFRLNWATFSTGDRRTDAGSDLSIFVGDLASDVTDAMLQETFATRYPSVKGAKVVTDSNTGRSKGYGFVRFGDENERSRAMNEMNGIYCSSRPMRIGVATPKKASGYQQQYSSQALVLAGGNASNGAVAQGSQANGDSTNTTIFVGGLDSEVTDEDLRQSFSQFGEVVSVKIPVGKGCGFVQFANRNSAEDALQRLNGTVIGKQTVRLSWGRNPASKQWRNDSNNQWNGAYYGGQGYGGGYGYAMPQNDPNMYAAAATYGAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AEX0</accession>
    <name>A0AA39AEX0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_001721</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily. AB hydrolase 4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AEX0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034338">
        <property type="term" value="F:short-chain carboxylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000140">
        <property type="entry name" value="Esterase/lipase/thioesterase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000952">
        <property type="entry name" value="AB_hydrolase_4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050960">
        <property type="entry name" value="AB_hydrolase_4_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10794">
        <property type="entry name" value="ABHYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10794:SF84">
        <property type="entry name" value="ESTERASE_LIPASE_THIOESTERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01133">
        <property type="entry name" value="UPF0017"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="141"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9706341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="435" mass="47684" checksum="7406A5D191E87A46" modified="2024-01-24" version="1">MIRGPQKYKQAMARAAWGSVTPPLTIFSPRSGLYTEPVRTQKIHFSVAAMLDGGQRRYDPSLEVLGGGLDSYLPAFKTLNRPYDAFPVVGWNRHVETISASFFRSVPGVRLRRECLRTKDDGAVALDWVSGDDRQLGADSPVLILLPGLTGGSEDSYVRHMLVRARSNGMRVVVFNSRGCADSPVTTAQFYSASFTEDLRQVVAHVANRYPNANLYAVGWSLGANILVRYLGQESHACPLSGAVSLCNPFNLVIADEDFRKGFNIVYDITLANALRRIFKKHTLLFEDIGGEFNIPLAANAKSVREFDDGLTRVSFGFKSVDDYYSNSSSSDSIKHVCTPLLCIQAANDPIAPSRGIPREDIKENPNCLLIVTPKGGHLGWVAGPEAPRGAPWTDPVVMDFLEHLERGRSKVIASSSESDTVQFCTEGLSHLKCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AF09</accession>
    <name>A0AA39AF09_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_003784</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AF09"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045894">
        <property type="entry name" value="At5g08430-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035445">
        <property type="entry name" value="GYF-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46851">
        <property type="entry name" value="OS01G0884500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46851:SF22">
        <property type="entry name" value="ZINC ION BINDING _ DNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55277">
        <property type="entry name" value="GYF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9705852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="99" mass="11229" checksum="69E8E23861C140C3" modified="2024-01-24" version="1">MGMMNVQTINFEDDGYDEKSLKVASKKQRSSKIKNEVWHCLGPLGERPGSFSLPLLRRWSEINANASKHKVWKVGQSKEHAIPLNDVISLILPIKSEKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AFJ6</accession>
    <name>A0AA39AFJ6_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_003482</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AFJ6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9705463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="108" mass="11838" checksum="BC5535AC89282549" modified="2024-01-24" version="1">MPLHETVGPLARNEWLLVMLPFQLASGRLVRGCLVVPFYQLTAAWLVPSALVDIQHGAVCLGKGLKNSPLIHCGENHRQKMKRLKLLMVHSCGVCFVVALVPASCIIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AFM1</accession>
    <name>A0AA39AFM1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 10">UDP-glucose 4-epimerase</fullName>
            <ecNumber evidence="10">5.1.3.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_001648</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>UDP-alpha-D-glucose = UDP-alpha-D-galactose</text>
            <dbReference type="Rhea" id="RHEA:22168"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:66914"/>
            <dbReference type="EC" id="5.1.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 10">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 10">Carbohydrate metabolism; galactose metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the NAD(P)-dependent epimerase/dehydratase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.-" evidence="10"/>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AFM1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003978">
        <property type="term" value="F:UDP-glucose 4-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006012">
        <property type="term" value="P:galactose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05247">
        <property type="entry name" value="UDP_G4E_1_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000040">
        <property type="entry name" value="UDP-glucose 4-epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.25.10:FF:000060">
        <property type="entry name" value="UDP-glucose 4-epimerase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005886">
        <property type="entry name" value="UDP_G4E"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01179">
        <property type="entry name" value="galE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007956">
        <property type="entry name" value="PRK10675.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43725">
        <property type="entry name" value="UDP-GLUCOSE 4-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43725:SF47">
        <property type="entry name" value="UDP-GLUCOSE 4-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16363">
        <property type="entry name" value="GDP_Man_Dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7" id="KW-0299">Galactose metabolism</keyword>
    <keyword evidence="8 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6 10" id="KW-0520">NAD</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-binding" evidence="11">
        <location>
            <begin position="6"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU366046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9706197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="349" mass="38218" checksum="DCC30B9B04BF2D1F" modified="2024-01-24" version="1">MAQSILVTGGAGYIGSHTVLQLLLGGFRAVVVDNLDNSSEIAIHRVKELAAEFGDNLVFHKLDLRDKPALEQLFASTNFDAVIHFAGLKAVGESVQKPLLYFDNNLIGTITLLEVMAAHGCKKLVFSSSATVYGWPKEVPCTEEFPLCATNPYGRTKLFIEDICRDIHRSDSEWKIVLLRYFNPVGAHPSGYIGEDPRGIPNNLMPFVQQVAVGRRPALTVFGSDYSTKDGTGVRDYIHVVDLADGHIAALHKLLNSEMAGCEVYNLGTGKGTSVLEMVAAFEKASGKKIPLVMAGRRPGDAEIVYASTTKAEKELNWKAKYGISEMCRDLWNWASKNPYGYESSPTTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AFZ6</accession>
    <name>A0AA39AFZ6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glucuronosyltransferase PGSIP8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_002060</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AFZ6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02537">
        <property type="entry name" value="GT8_Glycogenin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050587">
        <property type="entry name" value="GNT1/Glycosyltrans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11183">
        <property type="entry name" value="GLYCOGENIN SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="2" id="KW-0464">Manganese</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041296616" description="Glucuronosyltransferase PGSIP8" evidence="4">
        <location>
            <begin position="24"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="299"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="340"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="368"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="396"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="422"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9706905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="473" mass="53957" checksum="41AA6625ABFAB204" modified="2024-01-24" version="1">MGRREKSVLGILKVLLLVSSAFGSEDGYRRSSQSQTHRNAYAAMMYMGTPRDYEYYVALRVMMRSLMKLKVEADLVVIASTDVPLRWVQALEQEDGVKVVRVKNMNNPYKNQDHFDMRFKLTLNKLYAWSLVDYDRVIMLDSDNLFLQKTDELFQCGQFCAVFINPCIFHTGLFVLQPSMEVFRSMLHELEIGRENRDGADQGFLASYFPDLLDQPMFHPPPNATKLDGNYRLPLGYQMDASYYYLKLRWTIPCGPNSVITFPSAPWLKPWYWWSWPVLPLGLSWHQQRLQTLGYNAEMPVVLMQWIVYLGVIAMAHLARSNLSKLCYRRVEKSSSSIQTGLKLLTMWSIVAAYVLPFFLIPRTVHPLLGWTLYLLGSFALSSIAMNAFLLPTLAVLTPGLGILGALLVMAFPWYSDGVVRALVVFAYAFCCAPIAWTSLVKIMTCLQVSLEKEVFFPKSGDSQPSPESIKLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AGN5</accession>
    <name>A0AA39AGN5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cucumisin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_001915</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the peptidase S8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AGN5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02120">
        <property type="entry name" value="PA_subtilisin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04852">
        <property type="entry name" value="Peptidases_S8_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000006">
        <property type="entry name" value="Subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034197">
        <property type="entry name" value="Peptidases_S8_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045051">
        <property type="entry name" value="SBT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041469">
        <property type="entry name" value="Subtilisin-like_FN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10795">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17766">
        <property type="entry name" value="fn3_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 7" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Peptidase S8/S53" evidence="8">
        <location>
            <begin position="38"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="Subtilisin-like protease fibronectin type-III" evidence="9">
        <location>
            <begin position="518"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6 7">
        <location>
            <position position="45"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6 7">
        <location>
            <position position="108"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6 7">
        <location>
            <position position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF17766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9706670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="652" mass="69906" checksum="00E32E938475E1A8" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AHB3</accession>
    <name>A0AA39AHB3_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_002609</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AHB3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03801">
        <property type="entry name" value="GT4_PimA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46686">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46686:SF5">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase subfamily 4-like N-terminal" evidence="3">
        <location>
            <begin position="95"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="2">
        <location>
            <begin position="312"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9707646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="493" mass="55213" checksum="9C0E3E39C07A6929" modified="2024-01-24" version="1">MAPNTKPKKGFLPPQTSLTTTLFFIVLFTVPALLLLHAPSTSISNTFSSHVSPWTGDLRVAEFAWNRLELGGSRAPPVVLKIAVFSRKWPIGTAPGGMERHAHTLHTALSRRGHRVHIFTSPPGDQRGAAALQEMNMQPNGSPTSSSPRIHCHEGEPGRWRYNKAWEQFVEENRQEPFDVVHSESVALPHWLAKTLPNLAVSWHGIALESLSSDIYQDLALRPPGEPISPGFNRSVQGVIPKVLNEIRFFHNYAHHVAISDSCGEMLRDVYQIPTKRVHVILNGVDEEDFRQDLQLGHQFRSRIGIPQNASLVLGVAGRLVKDKGHPILHEAFSRFIKRHPDAYLIVAGSGPWENRYKDLGRQVLVLGSMNPSQLRAFYNSIDVFVNPTLRPQGLDLTLMEAMMSGKAVLASRFPSIKGTIVVDDEYGFMFSPNVESLLEAMEQVVKEGRKRLAQRGKACRRYATSMFTATKMALAFERLFLCIKNETFCMYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AM65</accession>
    <name>A0AA39AM65_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_000704</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AM65"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09917">
        <property type="entry name" value="F-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053301">
        <property type="entry name" value="F-box_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006597">
        <property type="entry name" value="Sel1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45088:SF1">
        <property type="entry name" value="OS04G0476000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45088">
        <property type="entry name" value="OSJNBA0022H21.17 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08238">
        <property type="entry name" value="Sel1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00671">
        <property type="entry name" value="SEL1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="50"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9708824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="335" mass="37751" checksum="FF2EE47FCABEBC8F" modified="2024-01-24" version="1">MKQRTWPVKSEGSRFTALRLSSGSGKKEETHLRSKRYSSITIPSRSSREETDFSKLPDDILQKITATFTLPDLQTASLVCRSWRDSLRPLREAMLLLKWGKRFKHGHGGVRPNIQKALDSFLKGAARGSTLAMVDAGLIYWEMGKKEESIALYRKAAELGDPTAQCNLGISYLHSERPKREEAAKWLYLSSNAGYVRAQYQLALCLHRGRGMDCNLPEAARWYLKAAEGGYVRAMYNVSLCYSYGEGLVHSHRQARRWMKRAADRGHSKAQFEHGLGLFSEGEMMKAVVYLELATRAGETAAAHVKNVILQQLSSTSRDRAMLLADNWRALPTSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D5T7</accession>
    <name>A0AA39D5T7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF4408 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_025716</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D5T7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008480">
        <property type="entry name" value="DUF761_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36887">
        <property type="entry name" value="OS01G0532300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36887:SF1">
        <property type="entry name" value="OS01G0532300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05553">
        <property type="entry name" value="DUF761"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9672191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="199" mass="21708" checksum="91DE177BD566D454" modified="2024-01-24" version="1">MDKAKKSQMLKLFLIAAVLFITPLLSSSLGTTYLYFIIINLLIIALGAEAGLLSFSKPPQDKKYAVPVSAKPLTHPDQAVPDKEASSNSTNTGVLLTTPEGTEKKAKVVEKSASEKIVGAAKLSKVRKCPSTPSLFFIGGGEAEAEEAVGEEDQEEVGGLSGQELFTKAETFIGNFHKQLKMQREESWKRLHEFYQKAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D818</accession>
    <name>A0AA39D818_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_023253</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D818"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.150.80:FF:000004">
        <property type="entry name" value="NAC transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.80">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003441">
        <property type="entry name" value="NAC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036093">
        <property type="entry name" value="NAC_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31719:SF183">
        <property type="entry name" value="NAC DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31719">
        <property type="entry name" value="NAC TRANSCRIPTION FACTOR 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02365">
        <property type="entry name" value="NAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101941">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51005">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NAC" evidence="7">
        <location>
            <begin position="7"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="172"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="172"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9673570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="299" mass="34138" checksum="0AA4B7697239C4B4" modified="2024-01-24" version="1">MTAELQLPPGFRFHPTDEELVMHYLCRKCASQSIAVPIIAEIDLYKYDPWQLPEMALYGEKEWYFFSPRDRKYPNGSRPNRAAGTGYWKATGADKPIGHPKPVGIKKALVFYAGKAPRGEKTNWIMHEYRLADVDRSARKKNNSLRLDDWVLCRIYNKKGIVEKQHTAARKSDCSDIEDQKPGPLALSRKAGVMPPPPPPSSSTAPTATAALDDLVYFDSSDSVPRLHTDSSCSEHVVSPEFTCEREVQSEPKWKEWENPMDFSYNYMDATVDNSFLSQFPNNQMSPLQDMFMYLQKPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DB90</accession>
    <name>A0AA39DB90_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Germin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_020728</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May play a role in plant defense. Probably has no oxalate oxidase activity even if the active site is conserved.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Secreted</location>
            <location evidence="2 13">Extracellular space</location>
            <location evidence="2 13">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the germin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DB90"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02241">
        <property type="entry name" value="cupin_OxOx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000005">
        <property type="entry name" value="Germin-like protein subfamily 1 member 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006045">
        <property type="entry name" value="Cupin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001929">
        <property type="entry name" value="Germin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019780">
        <property type="entry name" value="Germin_Mn-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31238">
        <property type="entry name" value="GERMIN-LIKE PROTEIN SUBFAMILY 3 MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00190">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00325">
        <property type="entry name" value="GERMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00835">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00725">
        <property type="entry name" value="GERMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 13" id="KW-0052">Apoplast</keyword>
    <keyword evidence="7 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 10 13" id="KW-0464">Manganese</keyword>
    <keyword evidence="6 10 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0964">Secreted</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041480119" description="Germin-like protein" evidence="13">
        <location>
            <begin position="22"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="Cupin type-1" evidence="14">
        <location>
            <begin position="62"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="31"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU366015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9678638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="218" mass="23003" checksum="3B3473A08FB6850D" modified="2024-01-24" version="1">MVVNTLAYIALLAVAFSLASASDPSPLQDFCVAVNDTNAAVFVNGQFCQDPKLATPNDFSFSGLRLPGNTSNQLGSMVTPATVAQIPGLNTLGISLARIDYAPYGLVPPHTHPRATEILTVLEGTLYVGFVTSDPDNRLISMVLYKGDVFVFPQGLIHFQLNVGTTNAVSISSLSSQNPGVITIASAVFGSKPAISVDVLTKAFQLDKDVVANLQSKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DBJ5</accession>
    <name>A0AA39DBJ5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Terpene synthase metal-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_024987</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9676252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DBJ5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010333">
        <property type="term" value="F:terpene synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005630">
        <property type="entry name" value="Terpene_synthase_metal-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03936">
        <property type="entry name" value="Terpene_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Terpene synthase metal-binding" evidence="1">
        <location>
            <begin position="2"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9676252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="50" mass="5693" checksum="77B0084FE5D13889" modified="2024-01-24" version="1">MITKEAFDWVYNDPFTVASCLIARLMNDMTTHTQMKANSSFFHMSACNGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DC74</accession>
    <name>A0AA39DC74_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Oleosin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_020807</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May have a structural role to stabilize the lipid body during desiccation of the seed by preventing coalescence of the oil. Probably interacts with both lipid and phospholipid moieties of lipid bodies. May also provide recognition signals for specific lipase anchorage in lipolysis during seedling growth.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Lipid droplet</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the oleosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DC74"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012511">
        <property type="term" value="C:monolayer-surrounded lipid storage body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019915">
        <property type="term" value="P:lipid storage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009791">
        <property type="term" value="P:post-embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048608">
        <property type="term" value="P:reproductive structure development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000136">
        <property type="entry name" value="Oleosin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33203">
        <property type="entry name" value="OLEOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33203:SF25">
        <property type="entry name" value="OLEOSIN 18.5 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01277">
        <property type="entry name" value="Oleosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00811">
        <property type="entry name" value="OLEOSINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="42"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="74"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="129"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="137"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9678731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="148" mass="15725" checksum="48EBD87973F6B18C" modified="2024-01-24" version="1">MAETQPPLHILHPHQHQHQPQAQQPRYQAVKAATAATAGGSLLVLSGLTLAGTVIALTIATPLLVIFSPVLVPAAIAVFFLVLGFLASGGFGVAAVTVLSWIYRYVTGRHPPGADQLESARMKLATKAREMKDRAEQFGQQHTGQQGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DDF9</accession>
    <name>A0AA39DDF9_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_021554</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DDF9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9679660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="80" mass="9029" checksum="B04B5944C855DC8E" modified="2024-01-24" version="1">MGSVAGFIRPMITLIRLISRNSLRWHGMGTNMYPSSSLLENIKGQMHALTNPLQLSPLLMVLLEWHRTLIVELQAGIRTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DDP5</accession>
    <name>A0AA39DDP5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Late embryogenesis abundant protein LEA-2 subgroup domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_021728</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DDP5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009506">
        <property type="term" value="C:plasmodesma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004864">
        <property type="entry name" value="LEA_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044839">
        <property type="entry name" value="NDR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31415:SF20">
        <property type="entry name" value="NDR1_HIN1-LIKE PROTEIN 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31415">
        <property type="entry name" value="OS05G0367900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03168">
        <property type="entry name" value="LEA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="26"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="Late embryogenesis abundant protein LEA-2 subgroup" evidence="6">
        <location>
            <begin position="80"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9679914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="210" mass="23119" checksum="553E7A675A5CE687" modified="2024-01-24" version="1">MSKLDSKSPKHCADKGLNIDKFYKKLYWSGFAFLFSVLSLILLVWLILHPTKPEFSLKEADIYQLSLSGPHLLNSSIQLTLLSKNPNTKVGIYYDMIQVYAAYKGQQITVDTSLPPFYQGYEESNLLTASLVGTGLPVAPSFGYEVGRDQTAGKLVLSLKLDGRVRWKVGTWVSGRYRLNVNCVAVMAFGPAIPSGPLSSKEGTQCSTSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DED6</accession>
    <name>A0AA39DED6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DYW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_020054</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. PCMP-H subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DED6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000452">
        <property type="entry name" value="pentatricopeptide repeat-containing protein At2g03880, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001093">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At2g34400"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000353">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At4g39530"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000073">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001355">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032867">
        <property type="entry name" value="DYW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF517">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14432">
        <property type="entry name" value="DYW_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="71"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="102"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="204"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="305"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="406"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="507"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="domain" description="DYW" evidence="4">
        <location>
            <begin position="722"/>
            <end position="814"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9680951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="814" mass="91582" checksum="049A6DD2AC211EC8" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DEH1</accession>
    <name>A0AA39DEH1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Axial regulator YABBY 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_019913</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the YABBY family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DEH1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010158">
        <property type="term" value="P:abaxial cell fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010154">
        <property type="term" value="P:fruit development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009944">
        <property type="term" value="P:polarity specification of adaxial/abaxial axis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000024">
        <property type="term" value="P:regulation of leaf development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902183">
        <property type="term" value="P:regulation of shoot apical meristem development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00084">
        <property type="entry name" value="HMG-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.10:FF:000047">
        <property type="entry name" value="Axial regulator YABBY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006780">
        <property type="entry name" value="YABBY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056775">
        <property type="entry name" value="YABBY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056776">
        <property type="entry name" value="YABBY_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31675:SF0">
        <property type="entry name" value="AXIAL REGULATOR YABBY 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31675">
        <property type="entry name" value="PROTEIN YABBY 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04690">
        <property type="entry name" value="YABBY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24868">
        <property type="entry name" value="YABBY_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="YABBY N-terminal" evidence="8">
        <location>
            <begin position="15"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="YABBY protein C-terminal" evidence="7">
        <location>
            <begin position="112"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF24868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9680740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="210" mass="23200" checksum="A5FAC55BC3D3DC6E" modified="2024-01-24" version="1">MSSSSAFSPDHLSPTSDQLCYVHCNFCDTVLAVSVPCTSLFKTVTVRCGHCTNLLSVNMRGLLLPAANQLHLGHSLFSPHNLLEEIRSPPSSMLINQPNPNEAVMPVRGVDEIPKPPVVNRPPEKRQRVPSAYNRFIKDEIQRIKAGNPGISHREAFSAAAKNWAHFPHIHFGLMPDQPVKKANVRQQEGEDMLMKDGFFAPANVGVSPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DES8</accession>
    <name>A0AA39DES8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">S-adenosyl-L-methionine-dependent methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_020196</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9681204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DES8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009902">
        <property type="entry name" value="DUF1442"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33593">
        <property type="entry name" value="DUF1442 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33593:SF1">
        <property type="entry name" value="DUF1442 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07279">
        <property type="entry name" value="DUF1442"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="184"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="201"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9681204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="238" mass="26407" checksum="6004C9F74C285531" modified="2024-01-24" version="1">MEWSVSSATNAYLDTLKLCNDEKRRQNSWKTPELESNEFISALAAGMKAKLIVEVTSGVSPSTIALAAAARQTGGRLVCILPERVLDESTKVIKDSGLRDMVEFKTGDPYELLPRYEKIDFSFVDCKTENYSRLVNVLDVNPRRSVVVANNLVGGKKGLGGHVKGLKDKVEVRSIGKGMEVTMIRRSDDTNQKSDCSGRGGEGRGHSQGERRRAKGKWIVKVDEESGEEHFFRMPRSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DET3</accession>
    <name>A0AA39DET3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_020208</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SDE2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9681219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DET3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17039">
        <property type="entry name" value="Ubl_ubiquitin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051421">
        <property type="entry name" value="RNA_Proc_DNA_Dmg_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053822">
        <property type="entry name" value="SDE2-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12786">
        <property type="entry name" value="SPLICING FACTOR SF3A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12786:SF1">
        <property type="entry name" value="SPLICING REGULATOR SDE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22782">
        <property type="entry name" value="SDE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="11">
        <location>
            <begin position="4"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="218"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="304"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="115"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="231"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="263"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="311"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="331"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="360"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="370"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9681219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="381" mass="41042" checksum="B1C79330FB90D4A7" modified="2024-01-24" version="1">MEIYHLFAKLLDGKTLTIRLTSPTISGDVIKGHLHRITSVPVHLQRLVSQSRQISDETLITADKGSGIFPTVHLLLPLPGGKGGFGSLLRGAATKAGQKKTNNFDACRDMSGRRLRHVNAEKKLEEWRAEAAERELERMAEDYIKKAAKKGKSGNGSAAAVAAEKYVEKYREESAKCVAEVEASVREAMGGFGKRKGAEVATSASDAKRLKIWMGKKKLEESDSDEDDAENERSIVLDHGSESDSNKETDGSFDSVSGGKLNGECSGGSCESGSEEEKETFGQVKPEVQEEMVVQATSVSCLDAVGSEAVEPEKGDSDEPKSGNVEVIGLPQSNSGSENGSDIEKVAIDSEDNGVSESKPGVHEKEKKAWQTQTPRMQESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DEU9</accession>
    <name>A0AA39DEU9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">LRAT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_020148</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9681115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DEU9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1720.10">
        <property type="entry name" value="endopeptidase domain like (from Nostoc punctiforme)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007053">
        <property type="entry name" value="LRAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46137">
        <property type="entry name" value="OS05G0310600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46137:SF2">
        <property type="entry name" value="OS09G0526800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04970">
        <property type="entry name" value="LRAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51934">
        <property type="entry name" value="LRAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LRAT" evidence="1">
        <location>
            <begin position="20"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9681115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="278" mass="29967" checksum="2B07C286C2CB373D" modified="2024-01-24" version="1">MGLLTNRVERSEIRPGDHIYTWRAVFTYSHHGIFVGGSKVVHFTPERNRDSNNGTPSDSYDLVSSIPSSCSTFPDCGFRQPNSGVVLSCLDCFLGKGSLYSFEYGVTPSVFLAKVRGGTCTTATSDPPDAVIHRAMYLLQNGFGNYDVFQNNCEDFALYCKTGLLIVDKSGVGRSGQASSVIGAPLAAILSSPLKLLMPSPVGVATVTAGMYCMSRYATDIGVRTDVIKVAVEDLAVNLGWGDVTEEVAEKMMLLIDRLSDHEEVAGNNEASNKQVAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DH29</accession>
    <name>A0AA39DH29_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Alkane hydroxylase MAH1-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_019092</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DH29"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11064">
        <property type="entry name" value="CYP86A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24296">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8 9" id="KW-0349">Heme</keyword>
    <keyword evidence="6 8 9" id="KW-0408">Iron</keyword>
    <keyword evidence="4 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="8">
        <location>
            <position position="454"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9683365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="508" mass="58559" checksum="19C725C21045C0E0" modified="2024-01-24" version="1">MTIGYLESFLAFLCFLLLWHWRQSRNLPMMKWPLLGMLPGLFCNVHRIHHYTTQLLKLCPTFEFKGPWFTSANFLITSDPANVRYMLSKNFSNFQKGSNFKKIFGEVLGDGIFTSESDSWATQRRLIHSVLKNGRFQQLFEKTTRTKVEKGLIPILEHVSMLGLEVDMQDLFSRFTFDNTCILILGLDPGILSIELREESYAKAFNEIEEALLYRHVLPESCWKLQKWLQIGKEKKMSGAVKVLEDFVFQCISLKQETLRGKRTRIKEDEEGSFDFLTAFMEKDGELTGGLEISDKFLKDTAINLLVAGQDTLSAAFSWFFWLIATHPLVENKIWEEIKANLREKDEETWWAFSAQEVSKLVYLHGALCETLRLFPPVPFEHKAPVKADILPSGHKIDPDTEVLFSLYAMGRMEDIWGPDCLEFKPERWISERGEIVHVPSHKFIAFNAGPRSCLGKEMTFTQMKIVAIAVICNYHVQLVEGHPVSPSISVVLHMRHGLKVRVSKRRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DH89</accession>
    <name>A0AA39DH89_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Response regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_016503</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Functions as a response regulator involved in His-to-Asp phosphorelay signal transduction system. Phosphorylation of the Asp residue in the receiver domain activates the ability of the protein to promote the transcription of target genes. Type-A response regulators seem to act as negative regulators of the cytokinin signaling.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ARR family. Type-A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DH89"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009736">
        <property type="term" value="P:cytokinin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17581">
        <property type="entry name" value="REC_typeA_ARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000159">
        <property type="entry name" value="Two-component response regulator ORR5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045279">
        <property type="entry name" value="ARR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43874">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43874:SF99">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR ARR16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0932">Cytokinin signaling pathway</keyword>
    <keyword evidence="1 8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="Response regulatory" evidence="9">
        <location>
            <begin position="22"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="8">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00043855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9684041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="153" mass="17200" checksum="ABCFDF0324665E99" modified="2024-01-24" version="1">MDAASSSTANDMMVMDYGDEPHVLAVDDNLIDRKLIEKLLKNFSCRVTTAENGLRALEYLGLGDDQQTTHKASVSKVNLIITDYCMPGMTGYELLKKVKQSSILKEVPVVIMSSENIPTRINKCLEEGAQMFMLKPLKQSDVKKLRCYLKHRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DHZ1</accession>
    <name>A0AA39DHZ1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">FMN-binding split barrel</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_016651</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DHZ1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.180.10">
        <property type="entry name" value="PNP-oxidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037119">
        <property type="entry name" value="Haem_oxidase_HugZ-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37375:SF1">
        <property type="entry name" value="DUF2470 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37375">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9684265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="309" mass="34330" checksum="652D6BC272E369EE" modified="2024-01-24" version="1">MKGNKASILTAAERCKNILASNWQGRLNTIKADAKGSKADIYSSKVNYMVKRGKPYIWVPENDLHNVNTIIDERGSFSVASPYPGPLANLLRSMKKFPARVALTGDVVPLKDEKVRFAEESLREMILSEQKAISEFSYSVSGILSSSGPSCPLRSENLQELLDGGEQHVIYKFNLSSCMFIDGNGGTHEVNLENIEASKADILSPFSANLIDGINQSEVRRRALLLFCIVYKNANAKDAYVLSIDRKGFEVLGKVPSPPMKDGFGEYQWKEFRFTFREEARSVEAFCSQLVEMEEEALKNVSSYSGLGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DHZ8</accession>
    <name>A0AA39DHZ8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Organelle RRM domain-containing protein 1, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_017065</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DHZ8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900871">
        <property type="term" value="P:chloroplast mRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016554">
        <property type="term" value="P:cytidine to uridine editing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080156">
        <property type="term" value="P:mitochondrial mRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000474">
        <property type="entry name" value="Organelle RRM domain-containing protein 1, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.80:FF:000007">
        <property type="entry name" value="Organelle RRM domain-containing protein 1, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039206">
        <property type="entry name" value="MORF/ORRM1/DAG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054059">
        <property type="entry name" value="MORF/ORRM1/DAG-like_MORF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31346">
        <property type="entry name" value="MULTIPLE ORGANELLAR RNA EDITING FACTOR 2, CHLOROPLASTIC-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31346:SF11">
        <property type="entry name" value="ORGANELLE RRM DOMAIN-CONTAINING PROTEIN 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21864">
        <property type="entry name" value="MORF_dom"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0934">Plastid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="317"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00069928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9684896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="409" mass="44812" checksum="4B968E8E9285FCCD" modified="2024-01-24" version="1">METLCPSATTFNITRKFSHKIQSPASALFHTALAFPSPFKNNYNNNLLNLSYSSSCSISCVSSSSSTATSALSASTTSTSLAFSASAGERQRHWMVQMEAPPPGLRSKAEIIDYYVRTLETVLGSEKDAQMCIYDASWDGPFGFCCDIDAETSCELSGLQGVLSVKPDPDFNSLKKDYTFSNIQLGSVSNSDIGSPQLFPAGNSKHWLVQMDRPTVGVVTKAQMVDFYAQILTKVLGNEKDAQMCIYHISWQSDFGFCCELDEECARELAGVPGVLSVRPDENFESNNKDYGGGSLQNSTDSLDSMEANQPTNIKTKKLFVTGLSFYTSEKTLRGAFEGFGELVEVKIIMDKISKRSKGYAFIEYTTEEAASAALKEMNGKIINGWMIVVDVAKPTPQKYSRSRPRPAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DL52</accession>
    <name>A0AA39DL52_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_013939</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NADH:flavin oxidoreductase/NADH oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9687954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DL52"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016629">
        <property type="term" value="F:12-oxophytodienoate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009695">
        <property type="term" value="P:jasmonic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031408">
        <property type="term" value="P:oxylipin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02933">
        <property type="entry name" value="OYE_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000073">
        <property type="entry name" value="12-oxophytodienoate reductase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001155">
        <property type="entry name" value="OxRdtase_FMN_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045247">
        <property type="entry name" value="Oye-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22893:SF112">
        <property type="entry name" value="12-OXOPHYTODIENOATE REDUCTASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22893">
        <property type="entry name" value="NADH OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00724">
        <property type="entry name" value="Oxidored_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0288">FMN</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH:flavin oxidoreductase/NADH oxidase N-terminal" evidence="7">
        <location>
            <begin position="12"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9687954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="398" mass="43759" checksum="EA7C4C9CDC89A33A" modified="2024-01-24" version="1">MAESSAEGTTTLFSPYKMGRFQLSHRVALAPMTRCRAMNGIPQPALAEYYSQRSTNGGFLITEGTLISPTAAGFPHVPGIYTGEQVEAWKKVVEAVHAKGAIIFCQLWHVGRASHQVYQPGGTGAPISSTSKPISGRWRILMPDGSHGKYPAPRALKTSEIPEVVEHYRQAALNAIAAGFDGIEIHGAHGYLIDQFLKDGINDRTDEYGGSISNRCKFLVQVVQAVAAAIGPDRVGVRVSPAIDHLEATDSNPLNLGLAVIERLNKLQVDWGSKLTYLHVTQPRYTAYGQTESGRHGSEEEEARLMRTWRRAYQGTFMCSGGFTRKLGMEAVALGDADLVSYGRLFISNPDLVLRLKVNAPLNRYIRAYFYTQDPVKGYTDYPFLNKGSESHEPLSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DN63</accession>
    <name>A0AA39DN63_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">cytokinin dehydrogenase</fullName>
            <ecNumber evidence="3">1.5.99.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_014059</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>N(6)-dimethylallyladenine + A + H2O = 3-methyl-2-butenal + adenine + AH2</text>
            <dbReference type="Rhea" id="RHEA:13625"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15825"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:17660"/>
            <dbReference type="EC" id="1.5.99.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oxygen-dependent FAD-linked oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.5.99.12" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DN63"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019139">
        <property type="term" value="F:cytokinin dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009690">
        <property type="term" value="P:cytokinin metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.462.10">
        <property type="entry name" value="FAD-linked oxidases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="entry name" value="Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016170">
        <property type="entry name" value="Cytok_DH_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015345">
        <property type="entry name" value="Cytokinin_DH_FAD/cytokin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016164">
        <property type="entry name" value="FAD-linked_Oxase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050432">
        <property type="entry name" value="FAD-linked_Oxidoreductases_BP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006093">
        <property type="entry name" value="Oxy_OxRdtase_FAD_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13878:SF127">
        <property type="entry name" value="CYTOKININ DEHYDROGENASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13878">
        <property type="entry name" value="GULONOLACTONE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09265">
        <property type="entry name" value="Cytokin-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55103">
        <property type="entry name" value="FAD-linked oxidases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00862">
        <property type="entry name" value="OX2_COVAL_FAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="9">
        <location>
            <begin position="63"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9688132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="521" mass="58293" checksum="D484E6E97756C609" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DPI6</accession>
    <name>A0AA39DPI6_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_013582</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ARG7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DPI6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009733">
        <property type="term" value="P:response to auxin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003676">
        <property type="entry name" value="SAUR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02519">
        <property type="entry name" value="Auxin_inducible"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9691449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="102" mass="12054" checksum="CE491FAA494C1DDE" modified="2024-01-24" version="1">MAFSVKRVKKIRQIMCLRQVMQRQKTTSVSAGHRRFVIPTWLLNLLIFVALLDKAEEEFGLQIERRPGFCFPVEWVLFFYRSTQVFLEKNEKKYGGPKAILC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2025-06-18" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DPN8</accession>
    <name>A0AA39DPN8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_011530</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03233">
        <property type="entry name" value="ABCG_PDR_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03232">
        <property type="entry name" value="ABCG_PDR_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000179">
        <property type="entry name" value="ABC transporter G family member 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000059">
        <property type="entry name" value="ABC transporter G family member 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029481">
        <property type="entry name" value="ABC_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034001">
        <property type="entry name" value="ABCG_PDR_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034003">
        <property type="entry name" value="ABCG_PDR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013581">
        <property type="entry name" value="PDR_assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48040:SF20">
        <property type="entry name" value="PLEIOTROPIC DRUG RESISTANCE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48040">
        <property type="entry name" value="PLEIOTROPIC DRUG RESISTANCE PROTEIN 1-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14510">
        <property type="entry name" value="ABC_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08370">
        <property type="entry name" value="PDR_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="521"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="554"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="605"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="639"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="664"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="749"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1202"/>
            <end position="1220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1291"/>
            <end position="1311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1338"/>
            <end position="1361"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="146"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="856"/>
            <end position="1108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9692516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1369" mass="154537" checksum="B3621AE36961AEC5" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DQA1</accession>
    <name>A0AA39DQA1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_012010</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9693098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DQA1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9693098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="206" mass="24180" checksum="4FC3F53E8852497D" modified="2024-01-24" version="1">MVFGRVPEKRSYSSESWRIGDRRRIEFVRVPENKRKKASYPGDYRRRDSIRASIGEYQTDKESNSCEYRRIQDRNDGIRSKIDEELNPCEYRRIKERKHPIRVTTEEEILSGRVSENTKQIGNRICVSTRESKTEMMVSDRVPEKRSFSSDYRSVTDRQGINPFDSCKIQDIKNGIQASTREEIVFGRAPGNRRHTKNRILASIGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DRQ3</accession>
    <name>A0AA39DRQ3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DC1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_010328</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DRQ3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004146">
        <property type="entry name" value="DC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47841:SF7">
        <property type="entry name" value="CYSTEINE_HISTIDINE-RICH C1 DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47841">
        <property type="entry name" value="DIACYLGLYCEROL KINASE THETA-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03107">
        <property type="entry name" value="C1_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="DC1" evidence="3">
        <location>
            <begin position="12"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="DC1" evidence="3">
        <location>
            <begin position="63"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="DC1" evidence="3">
        <location>
            <begin position="120"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="264"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="268"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9694796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="310" mass="34216" checksum="8EA57707A1522ABE" modified="2024-01-24" version="1">MKPNIIEHYTNPGHSLTEVYGSEEFHCDGCKTAGNGPRYRCHTCDSTLHEYCGVCPSTLSTFMHPQHQLERVIRTQGRRVNERLCNLCRDDVEGLFYRCTHCDFDIHPLCTKIPEYVPHPLHPTHQLKLQTSSSGWCSVCKRECTSWRYGCRICSFDIHLECILAPCETPKKPRSVGAPATPAPPPYGPYGIAPPLHPWGVYGGLPPYSAYGPWYPYYSSTPHLYNWPSPTPFSAYAHACAYPPPPPSSFLVPLPSPGFYAHGGTTDFGPNNTNNQAQGTGKPSKGRLSKIIHALSMGVLCNLIAGGITG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DSE7</accession>
    <name>A0AA39DSE7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_010082</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C85 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DSE7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22751">
        <property type="entry name" value="OTU_plant_OTU9-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.80:FF:000001">
        <property type="entry name" value="OTU domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003323">
        <property type="entry name" value="OTU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050704">
        <property type="entry name" value="Peptidase_C85-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12419">
        <property type="entry name" value="OTU DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12419:SF112">
        <property type="entry name" value="OTU DOMAIN-CONTAINING PROTEIN DDB_G0284757-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02338">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50802">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="OTU" evidence="6">
        <location>
            <begin position="193"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9694419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="338" mass="38672" checksum="40BD296B25E7090C" modified="2024-01-24" version="1">MVIHDQDLDVVQWGLSLFGGDPDSGYCCNPAEHDTNIYGQYFRDHYETECSVENDERIAHALQEEFSQLAITEASESSHAGEEHVQAPIYPQDWLALPAGNYCSAHQDDREEADHIVPSGSCSSPEERLCDGEAYSYSLEITDESALDGEVGKRLNQIIPAPHVPRINGEIPSVDEATSDHQRLLDRLQHFGLFEFKVEGDGNCQFRALSDQFYRTPEHHKFVRRQVVNQLKSHPEIYEGYVPMAYDDYLKKMSRSGEWGDHVTLQAAADSYGVKIIIFTSYKDSSNIEILPKAPKSNRVIYLSFWAEVHYNSIYPQEESLSYESKKKKRWWIFGNKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DSM0</accession>
    <name>A0AA39DSM0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Agamous-like MADS-box protein AGL15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_011045</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Probable transcription factor involved in flower development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DSM0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00265">
        <property type="entry name" value="MADS_MEF2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1810.10:FF:000003">
        <property type="entry name" value="MADS-box transcription factor MADS-MC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050142">
        <property type="entry name" value="MADS-box/MEF2_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033896">
        <property type="entry name" value="MEF2-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002487">
        <property type="entry name" value="TF_Kbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48019">
        <property type="entry name" value="SERUM RESPONSE FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01486">
        <property type="entry name" value="K-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00404">
        <property type="entry name" value="MADSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51297">
        <property type="entry name" value="K_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00350">
        <property type="entry name" value="MADS_BOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="8">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="K-box" evidence="9">
        <location>
            <begin position="122"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="206"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="231"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00054254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9695876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="286" mass="32735" checksum="9BCF39579856A184" modified="2024-01-24" version="1">MGRGKIEIKKIENINSRQVTFSKRRAGLLKKAKELSVLCDAEVGVIIFSSTGKLYEFSSSSMEHTLTRYGRGLDSELPSLHRSAEVRYSIVPQVISRVLEIVYKNIIFMYVKFTILVVFTFEQESQPEIDSLKEEVAKLQKGYLRMMGKELDGLSFKELQHLEHLLSEGILSVKDKKEQVLLEQLKKSRMHEQRAMMENETLRKQVEELRRSSRPQPPSFIEYHPPERRQSLQISSKTPCSCSTVDERGGSDTSLHLGLPSDAYLKRKGPKIEPIFIDSESQVASE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DTI9</accession>
    <name>A0AA39DTI9_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_008568</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DTI9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9696413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="102" mass="11240" checksum="F04F79F611D3B845" modified="2024-01-24" version="1">MSVLLFQGLCSSNVPCSVPSDMETAARPWIFPDNSSNAECLVCNMLSLLLRDPCKGNSPLPQSNALVWIGCFYLIRNIRSCLVTVFENCFLIFCKTNVSLGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DVH4</accession>
    <name>A0AA39DVH4_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_007986</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SHI protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9699161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DVH4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009851">
        <property type="term" value="P:auxin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007818">
        <property type="entry name" value="SHI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006511">
        <property type="entry name" value="SHI_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006510">
        <property type="entry name" value="Znf_LRP1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01624">
        <property type="entry name" value="LRP1_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01623">
        <property type="entry name" value="put_zinc_LRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31604">
        <property type="entry name" value="PROTEIN LATERAL ROOT PRIMORDIUM 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31604:SF30">
        <property type="entry name" value="PROTEIN LATERAL ROOT PRIMORDIUM 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05142">
        <property type="entry name" value="DUF702"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="6" id="KW-0073">Auxin biosynthesis</keyword>
    <keyword evidence="10" id="KW-0927">Auxin signaling pathway</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="184"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="298"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="184"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="205"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="221"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="301"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9699161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="347" mass="35985" checksum="8E05C53860B871AC" modified="2024-01-24" version="1">MRWAWWVFATSSSSLRPPPSTTTTSTTPSCPILTPSMARMQPPLLVLGSASVSFPLLTAAPCLAPASMGGVEEADMLSHRSKGGGIQLWQNQQTQHANYLKKPMILDHNSSSNLLSCGVGGVGASGPTTAGGTTCQDCGNQAKKDCSHRRCRTCCKSRGFDCATHVKSTWVPAARRRERQLMVATTPGAGSSGSTSGAKKPRLITSQTTTTSHTSTSNTTPPRSFDTSSSHQDASFKEALPGQVRAPAVFKCVRVTAVEDGEDEYAYQAVVKIGGHVFKGFLYDQGVETRDGFPNISELHLGGGSGSGGGGGGRNGGTSSPILDPPDVYAASGGGLLGGSSYGNAIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DW87</accession>
    <name>A0AA39DW87_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_007478</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DW87"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31437:SF1">
        <property type="entry name" value="PROTEIN SREK1IP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31437">
        <property type="entry name" value="SREK1IP1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13917">
        <property type="entry name" value="zf-CCHC_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="6">
        <location>
            <begin position="87"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="33"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="122"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="46"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="130"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="164"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="175"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="200"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="227"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="256"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="293"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="311"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9698409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="329" mass="37076" checksum="7DE46178E47BCDA6" modified="2024-01-24" version="1">MPATAGRVRMPANNRVHSSAALQTHGIWQSAIGYDPYAPNKDDAKNSSQPKSSNSEPEAENAYASFQGLLALARITGSNADETRGACKKCGRVGHLTFQCRNFLSIKDDKEKDPDAIQASVLSGLDKLKGNVGKKVNGKGTVEDESESEEEDESESSDSDADSEIERIIAERNGKKGGSKGRSSRKKEVSDDDGSDSRERKKRGRSKRRSGKRVSGDLDNDDDESRRKRRKEKRRKRNDSSDEEKSSDEDKEERRQRRRKSRKEKRRRRSHRYSDDSDSSGDSDRHKRKSRRAASLSDSDISGSDDSRVGRGTKRSEKRNRKRHHKHDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E0B3</accession>
    <name>A0AA39E0B3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">1-phosphatidylinositol 4-kinase</fullName>
            <ecNumber evidence="3">2.7.1.67</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_003061</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PI3/PI4-kinase family. Type III PI4K subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.67" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004430">
        <property type="term" value="F:1-phosphatidylinositol 4-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05167">
        <property type="entry name" value="PI4Kc_III_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1070.11:FF:000012">
        <property type="entry name" value="Phosphatidylinositol 4-kinase alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1010.10:FF:000012">
        <property type="entry name" value="Phosphatidylinositol 4-kinase alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.70">
        <property type="entry name" value="Phosphatidylinositol 3-kinase, accessory domain (PIK)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015433">
        <property type="entry name" value="PI3/4_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001263">
        <property type="entry name" value="PI3K_accessory_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042236">
        <property type="entry name" value="PI3K_accessory_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045495">
        <property type="entry name" value="PI4K_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048:SF15">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-KINASE ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00613">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19274">
        <property type="entry name" value="PI4K_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00145">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00915">
        <property type="entry name" value="PI3_4_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51545">
        <property type="entry name" value="PIK_HELICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PIK helical" evidence="9">
        <location>
            <begin position="1329"/>
            <end position="1526"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="8">
        <location>
            <begin position="1597"/>
            <end position="1875"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="189"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="189"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="207"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="230"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9704831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1891" mass="208460" checksum="3E67DD8FDCA22A79" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E3V1</accession>
    <name>A0AA39E3V1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_002444</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E3V1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9707423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="75" mass="8471" checksum="C9C6F36B88E24F1F" modified="2024-01-24" version="1">MGRVEEDVTIAMVVVGASDGDDIPLDNEQWSGYKRFKKGEERLKKGSEKNLKELEREPEMISTGGGFTLVRGYYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E3X7</accession>
    <name>A0AA39E3X7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein OBERON 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_000577</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E3X7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010078">
        <property type="term" value="P:maintenance of root meristem identity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010492">
        <property type="term" value="P:maintenance of shoot apical meristem identity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010071">
        <property type="term" value="P:root meristem specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15612">
        <property type="entry name" value="PHD_OBE1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047578">
        <property type="entry name" value="OBE1-like_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004082">
        <property type="entry name" value="OBERON"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032881">
        <property type="entry name" value="Oberon-like_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032535">
        <property type="entry name" value="Oberon_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21736:SF38">
        <property type="entry name" value="PROTEIN OBERON 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21736">
        <property type="entry name" value="VERNALIZATION-INSENSITIVE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16312">
        <property type="entry name" value="Oberon_cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07227">
        <property type="entry name" value="PHD_Oberon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01544">
        <property type="entry name" value="ARATH130DUF"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Oberon-like PHD finger" evidence="9">
        <location>
            <begin position="457"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="domain" description="Oberon coiled-coil region" evidence="10">
        <location>
            <begin position="680"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="133"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="171"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="306"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="649"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="722"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="14"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="134"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="188"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="326"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9708611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="820" mass="92438" checksum="3F7A733134CD0C8B" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E4X9</accession>
    <name>A0AA39E4X9_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_001992</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E4X9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47951:SF3">
        <property type="entry name" value="CYTOCHROME P450, FAMILY 706, SUBFAMILY A, POLYPEPTIDE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47951">
        <property type="entry name" value="OS08G0547900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9706795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="391" mass="43931" checksum="F0ADE4D13B6F8371" modified="2024-01-24" version="1">MDEFVRLTLTLAIVILASSWFKWMFYERPRPPLPPGPRCLPVVGHLPFRDLEFHSYFARLARIHGPIFHLRNELGVVVTSPSLARQGLREGHYLGQQRRAGGGLGHVVRPLLLTMLRRVCVSQMLGSATLNAFYDLRRREVHRMLSYLYSQASPVNVGEQMFLTIMNVMTSMLWRGTVKGGERESLAAEFKQVVAEMTELLGKPNISDFFPGLAPFDLQGIKKWFKALATKLDGVFDPIIAQRVKMDKEGGSIEGKAGKDFLQFLLNLKDEGDSKTPLKMAHVKALLIDMVVGGTDSTSNTLKFAIADMMNKPEVMKKAQQELDVVVGKDSIEEESHIYRLPYLSAVMKEAMRLHPMLPLMVPHCPSETCVVGGYTIPKGVRIFVNVWAIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E8A9</accession>
    <name>A0AA39E8A9_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_001258</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E8A9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31623">
        <property type="entry name" value="F21J9.9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31623:SF79">
        <property type="entry name" value="SALUTARIDINOL 7-O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9709689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="433" mass="47889" checksum="5985F1FB87F3AD31" modified="2024-01-24" version="1">MEMKIDILSTQPIKPSSPTPDHLRSFKISLLDQLAPPFYVPVILFFSADDFDCEAVDHVTICDLLKRSLSRTLSRFYPLAGKLKGNDSVDCNDDGAVFMEARANVELSEILRDPEMDLLQKLLPCEPYSVGSESSDRAITAIQATIFECGGIGIGVCMSHKVADGATLATFLTAWSATAMGTDDGITPFLDSASLFPPRDINMVVPSGVISHDKILTRRFIFDAASLARLQSKASNSTRVLAVTSLIWKSAMAVAREKSGKDTISSIVTHVVNLRGKTEPPLSDRSLGNLWQSAVAPITEEEGKVELDDLVGRLRRAIKKVDKEYVKELQGEEGLSKAFEAMKEVRKMIMSNGEMELYRFSSWSRFPFYEIDFGFGRPIWVCTTNVPIKNVIILMGTKSGGGIEAWVTMAEEDMTKFQRHYELLEFVSSTQSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39EE11</accession>
    <name>A0AA39EE11_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_001458</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39EE11"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051358">
        <property type="entry name" value="TF_AMS/ICE1/BHLH6-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31945:SF143">
        <property type="entry name" value="HELIX-LOOP-HELIX DNA-BINDING DOMAIN CONTAINING PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31945">
        <property type="entry name" value="TRANSCRIPTION FACTOR SCREAM2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="6">
        <location>
            <begin position="326"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="299"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="303"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9709987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="363" mass="38939" checksum="86EA5006FF37B2A9" modified="2024-01-24" version="1">MLPRSNDIVWTRSDAVSWSKNSNDNRVGDGGGDGERMSSVSALKAIVEEEWLVSGVEMKGNLVSQSQSGAVNSLLHPMDCSSSCSPASGFGLDPAFQGKSCLSSMVCGYPFDGSFDLVCDPGFLEPIPEIQVPSSSSLMGFNDLASQPLMGASNLGSNLQFPATHLAGNGGSTATGGFNPSGFEGLVGSSLFVDRCKVLKPLENFPSVGSQPTLFQKRAILRRNSTERAGNYGVSGQEGSAIPVRVAGEDKGKRPVVEEEMDKMRKDKSDDEDDMDEASIARSGLIYDSDDAIENHKVEETANDGGDNSNLNGSSIGGNRKGKKKGLPAKNLMAERRRRKKLNDRLYMLRSVVPKISKVRIFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YH00</accession>
    <name>A0AA38YH00_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_026680</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NRAMP (TC 2.A.55) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YH00"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015086">
        <property type="term" value="F:cadmium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034755">
        <property type="term" value="P:iron ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00221">
        <property type="entry name" value="NRAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001046">
        <property type="entry name" value="NRAMP_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01197">
        <property type="entry name" value="nramp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037982">
        <property type="entry name" value="Nramp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11706:SF99">
        <property type="entry name" value="METAL TRANSPORTER NRAMP5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11706">
        <property type="entry name" value="SOLUTE CARRIER PROTEIN FAMILY 11 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01566">
        <property type="entry name" value="Nramp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00447">
        <property type="entry name" value="NATRESASSCMP"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="119"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="152"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="242"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="337"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="362"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="396"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="438"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="467"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="475"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9670278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="510" mass="54883" checksum="4EDE4092D08EC611" modified="2024-01-24" version="1">MALVQIQDTPLWKKLLSYVGPGFLVSVAYLDPGNLETDLQAGANHKYQLLWIVLVGLIFAFIIQSRSANLGVATGKHLSEHCKMEYPKPVNYCLWMLAEVAVIAADIPEVIGTAFAINILFHVPIWGGVLLAGLSTLLLLGLQRYGIRKLEVAIGVLVLVVGGCFCSVMAHARPSVKDIATGLFVPKLNGDGATRDAIALLGALNMPHNLFLHSALVTSRKIPHSFDAIRSASKYFIIESGLALFIALLINVAVVSVSGSVCSNPHASSQNKDHCEDITLESASFLLNNALGNWSSKLYAVSLLASGQSSTVTGTYAGQYIMQGFLDLRMTLWLRNLVTRCIAIAPSLMVCIIGGSSGAAKLIIIASMILSFELPFALIPLLRFTSSKAKMGDHKSSIMVTLLSWLLGFCSIGINIYFLSSSLIGWVTNYDTPKTGSILAGVVIFPVMILYAAMLFYLTIKRESSDSSTRGSEQLTNNTPTTDTTGLEGGTKFSSTQRAHEIEVVDIAYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YHX2</accession>
    <name>A0AA38YHX2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fungal lipase-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_026958</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YHX2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00519">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002921">
        <property type="entry name" value="Fungal_lipase-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044819">
        <property type="entry name" value="OBL-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46086">
        <property type="entry name" value="ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46086:SF17">
        <property type="entry name" value="ALPHA_BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01764">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fungal lipase-type" evidence="2">
        <location>
            <begin position="230"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9670743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="499" mass="57410" checksum="66A0541D5440DC3F" modified="2024-01-24" version="1">MWGWGKEKFYLMSLPTFFFPFCNNLAMATKECNNEFSSERVLLTPEEVSFFQLFRILFPGDKGKENFVDSQKGVESTFKRRWIIFISILVQKFLQSVAKPLSWFGSKFETGLNLSSSNGSFRMLLLNCLRGKIQWPDKTSSTFLSFNGHLDKRVELDKSIKPGDSRYYAALSVMSAKASYENEAYIKTTVEDHWKMEFLGFFDFWNDYQEKATTQAFILRDKNGGSDTIIVAFRGTETFDADAWCTDFDLSWYEIPGVGNIHGGFMKALGLKKNLGWPKEIKQDDSRPQVAYYAIREMLRERLKASDQTKFLVTGHSLGAALAILFPAVLVLHEEGWMLDRLLGVYTFGQPRVGDHKFGEFMTEQLKKHSIPYFRFVYSNDLVPRLPYDDTALMFKHFGTCLYFDRSYEGKIVAEEPNKNYFSPLMAMPKTLNAMRELIRSFTIARSKGKEYAEGWFLRFFRVLGLIVPGVSAHGPQDYVNSTRLGSPTLFLPHHDPTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YII8</accession>
    <name>A0AA38YII8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RCC1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_027180</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9671061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YII8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058923">
        <property type="entry name" value="RCC1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051210">
        <property type="entry name" value="Ub_ligase/GEF_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22870">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22870:SF360">
        <property type="entry name" value="ULTRAVIOLET-B RECEPTOR UVR8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00415">
        <property type="entry name" value="RCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25390">
        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="35"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="88"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="RCC1-like" evidence="4">
        <location>
            <begin position="117"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="140"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="192"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="246"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="298"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="350"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="414"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="417"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="438"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9671061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="447" mass="47980" checksum="DFEE95E96F581146" modified="2024-01-24" version="1">MADVGDTMSEVSAPVRRVLLISAGASHSVALLSGNVVCSWGRGEDGQLGHGDAEDRLSPTYLSALDGHEVVSVTCGADHTTAYSESFAQVYSWGWGDFGRLGHGNSSDLFTPQPIKALHGLRIKQIACGDSHCLAVTMDGEVQSWGRNQNGQLGLGTTEDSLVPQKIQAFQGVSVKMVAAGAEHTAAVTEDGELYGWGWGRYGNLGLGDRNDRLVPEKVSTVEVQGVKMVKVACGWRHTISVSSSGGLYTYGWSKYGQLGHGDFEDHLTPHKLEALQENLISEISGGWRHTMALTSDGKLYGWGWNKFGQVGVGDNVDHCSPVQVKFPHEQKVVHISCGWRHTLAVTERQNVFSWGRGTNGQLGHGESIDRNIPKMIEVLSADGSGGQQIEASKVDPSTGRIWVSPAERYAVVPDETGSTQTSVSVKGNGNDASVPESDVKRMRLAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YKB3</accession>
    <name>A0AA38YKB3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">COBRA C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_025619</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the COBRA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YKB3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010215">
        <property type="term" value="P:cellulose microfibril organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056900">
        <property type="entry name" value="COB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006918">
        <property type="entry name" value="COBRA_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31052:SF2">
        <property type="entry name" value="COBRA-LIKE PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31052">
        <property type="entry name" value="COBRA-LIKE PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25079">
        <property type="entry name" value="COB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04833">
        <property type="entry name" value="COBRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041204392" description="COBRA C-terminal domain-containing protein" evidence="9">
        <location>
            <begin position="30"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="domain" description="COBRA C-terminal" evidence="10">
        <location>
            <begin position="425"/>
            <end position="634"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF25079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9672049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="660" mass="73822" checksum="70503307359A68F5" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YKF0</accession>
    <name>A0AA38YKF0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Beta-amyrin 28-monooxygenase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Beta-amyrin 28-oxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_025653</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>beta-amyrin + 3 reduced [NADPH--hemoprotein reductase] + 3 O2 = oleanolate + 3 oxidized [NADPH--hemoprotein reductase] + 4 H2O + 4 H(+)</text>
            <dbReference type="Rhea" id="RHEA:43068"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:10352"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:82828"/>
            <dbReference type="EC" id="1.14.14.126"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YKF0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102373">
        <property type="term" value="F:beta-amyrin 28-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016709">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016125">
        <property type="term" value="P:sterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11043">
        <property type="entry name" value="CYP90-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000022">
        <property type="entry name" value="Taxadiene 5-alpha hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286:SF190">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286">
        <property type="entry name" value="CYTOCHROME P450 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 13" id="KW-0349">Heme</keyword>
    <keyword evidence="8 12 13" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 12 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041310921" description="Beta-amyrin 28-monooxygenase" evidence="14">
        <location>
            <begin position="22"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="12">
        <location>
            <position position="419"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00052804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00073578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00083450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9672088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="473" mass="53686" checksum="13C38721EE2C565C" modified="2024-01-24" version="1">MSAFFTIFLFLLPIFLLLVRRRSLPKGVPPGSLGLPLIGQSIGLLRAMRANTAEKWLEERIKKYGPISKLSLFGQPAVFIYGQAANKLVFASDGSTISNQQAKSNQMILGDRNLLELSGEDHKRVRGAIVSFLKPESLKQYVGKMDAEVRKHLEMHWQGKQRVTVMPLMKTLTFNIICGLLFGVERGIRREKFVARFQEMIEGIWSVPVNLPFTRYNRSLQASTKIQNMIKELMKEKDVELEKGASPHQDLITCLLSIRGKNNEVITEKEIVDNVLLVMVAGHDTSAVLITFLVRLLANDPDVYAAVLKEHEEIAKGKPSGEFLTWEDLAKMKYTWRVALETLRMFPPVFAGFRTVLKDIEFEGYLIPEGWKIFWATSMTHMNNSIFPEPTKFDPTRFENQASIPPYCFIPFGGGPRICPGIEFARIETLVTIHHLVTRFKWKLCHTDNFFSRNPTPAPTGGLPIEITSMNLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YKF3</accession>
    <name>A0AA38YKF3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AT hook motif-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_025666</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YKF3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045881">
        <property type="entry name" value="MNM1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34682">
        <property type="entry name" value="AT HOOK MOTIF-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34682:SF1">
        <property type="entry name" value="PROTEIN METABOLIC NETWORK MODULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="380"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="401"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9672109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="418" mass="45229" checksum="A0F70C63E6661793" modified="2024-01-24" version="1">MNEANQGSNPDFPGNFPVKRKRGRPRKDQSKKRGENARIPPGFEGVDANLPLQVEPINVVNDAMVGQVVSGVVDASFDAGYLLTVRVGNSDTILKGVVFKPGRYVPISSENDVAPHVQMIRRNEIHLPAGNQSQEQGYNPQARERNEQHGNFQGNDTAHPWNGSPPANHVPRVVPRAANVAVSKGKQVQTVVTQTVAHPVGERGTLVPVVLPPASQSNEAPPVNLVPPPDIQAANPAASKGKQVLAAALQGPHSSNGSTSNSQISAVAKVMKNEDTFHQTPAEVVYDAECRSMRMPNMPFETLLGEVVKRIQVPSQLEEGRIQDIQDSGRNSEEGHNMNQALIVKPLQAVQPNHHDHTASAPQHLDNNMTSRMTELLQALPDYVRESQVPGVQDAATSSGHDFDEPRNQEEATGDSLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YL69</accession>
    <name>A0AA38YL69_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">K Homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_025917</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YL69"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048024">
        <property type="term" value="P:regulation of mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045071">
        <property type="entry name" value="BBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055256">
        <property type="entry name" value="KH_1_KHDC4/BBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032377">
        <property type="entry name" value="STAR_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11208:SF104">
        <property type="entry name" value="OS02G0722700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11208">
        <property type="entry name" value="RNA-BINDING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22675">
        <property type="entry name" value="KH-I_KHDC4-BBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16544">
        <property type="entry name" value="STAR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="3">
        <location>
            <begin position="136"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="264"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9672493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="287" mass="31538" checksum="D64114CEF469FDA6" modified="2024-01-24" version="1">MSSGRYMAYSPSPSTAPHSPHIAGLRSATSALVEQEKYLSELLAERHKLSPFMPVLPHSYRLLNQEILRVTTLLGNASILDQSGLEHASPLASGGIFSNGGANVNGWASPFQSEMSGLSAAQNWLGSQGSSSGLIVKRTIRVDIPVEKFPSYNFVGRLLGPRGNSLKRMEASTECRVLIRGRGSIKDPAREEMMRGKPGYEHLNEPLHILVEAELPVEIVDARLMQAREILEDLLKPVDESHDFFKKQQLRELAMLNGTLREEGSHMSGSVSPFHNSLGMKRAKTRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YM09</accession>
    <name>A0AA38YM09_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_026186</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YM09"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9672839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="70" mass="8227" checksum="543BF30DD38DDFAE" modified="2024-01-24" version="1">MRKFRMIMRIGTETHFSHFQSYEYSPVFGMSSPFFGMNLQTGDGPLKLFFFSCFMHSIHLESPSKHKSKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YM71</accession>
    <name>A0AA38YM71_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Disease resistance protein RPS5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_026333</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9673011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YM71"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.430:FF:000003">
        <property type="entry name" value="Probable disease resistance protein At5g66910"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050905">
        <property type="entry name" value="Plant_NBS-LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463:SF220">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NB-ARC" evidence="8">
        <location>
            <begin position="154"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="9">
        <location>
            <begin position="409"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="10">
        <location>
            <begin position="556"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="32"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9673011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="892" mass="103734" checksum="9D778A2BB898A5BA" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YN43</accession>
    <name>A0AA38YN43_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">PISTILLATA-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_023225</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YN43"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00265">
        <property type="entry name" value="MADS_MEF2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050142">
        <property type="entry name" value="MADS-box/MEF2_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033896">
        <property type="entry name" value="MEF2-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002487">
        <property type="entry name" value="TF_Kbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48019">
        <property type="entry name" value="SERUM RESPONSE FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01486">
        <property type="entry name" value="K-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00404">
        <property type="entry name" value="MADSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51297">
        <property type="entry name" value="K_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00350">
        <property type="entry name" value="MADS_BOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="7">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="K-box" evidence="8">
        <location>
            <begin position="84"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="77"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9673534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="212" mass="24836" checksum="0E8DFD7D8374E56D" modified="2024-01-24" version="1">MGRGKIEIKRIENSSNRQVTYSKRRNGIMKKAKEITVLCDAHVSLVIFASSGKMHEYCSPSTTLIDILDRYHKQSGKRLWDAKHENLSNELDRIKKENDSMQIELRHLKGEDISSLHHKELMAIEDALEIGLASVRNKQMEFYKMVKKNQRILEEENKHLNYIVHHQGMPMEAGNVREVESGYHQRTVRDYNPQMPFAFRVQPIQPNLQERI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YN59</accession>
    <name>A0AA38YN59_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_023184</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YN59"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.80">
        <property type="entry name" value="Ribosomal protein S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001971">
        <property type="entry name" value="Ribosomal_uS11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036967">
        <property type="entry name" value="Ribosomal_uS11_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11759">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S14/30S RIBOSOMAL PROTEIN S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00411">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="11"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9673472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="95" mass="10217" checksum="33CF98328EE3A717" modified="2024-01-24" version="1">MSSEEAPAETVLERRVREPKEENATFGPTRDLVRTTGGMKEKTDGDESSPYAVMPATQDVSQGCNELDVNVLHVKLRAAGGNKTKTLGPDTQSAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YPP8</accession>
    <name>A0AA38YPP8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Subtilisin-like protease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_023744</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the peptidase S8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YPP8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02120">
        <property type="entry name" value="PA_subtilisin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04852">
        <property type="entry name" value="Peptidases_S8_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000006">
        <property type="entry name" value="Subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.80:FF:000003">
        <property type="entry name" value="Subtilisin-like protease SBT1.9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000039">
        <property type="entry name" value="Subtilisin-like protease SBT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.2310:FF:000003">
        <property type="entry name" value="Subtilisin-like protease SBT4.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034197">
        <property type="entry name" value="Peptidases_S8_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010259">
        <property type="entry name" value="S8pro/Inhibitor_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045051">
        <property type="entry name" value="SBT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041469">
        <property type="entry name" value="Subtilisin-like_FN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10795">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17766">
        <property type="entry name" value="fn3_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05922">
        <property type="entry name" value="Inhibitor_I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 12" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 12" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041344477" description="Subtilisin-like protease" evidence="13">
        <location>
            <begin position="33"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="domain" description="Inhibitor I9" evidence="15">
        <location>
            <begin position="39"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="14">
        <location>
            <begin position="142"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="domain" description="Subtilisin-like protease fibronectin type-III" evidence="16">
        <location>
            <begin position="662"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="11 12">
        <location>
            <position position="151"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="11 12">
        <location>
            <position position="224"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="11 12">
        <location>
            <position position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF05922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF17766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9674376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="19">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="766" mass="82866" checksum="3E253FFEAFD35094" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YSS6</accession>
    <name>A0AA38YSS6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein NRT1/ PTR FAMILY 5.10-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_024763</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YSS6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009705">
        <property type="term" value="C:plant-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071916">
        <property type="term" value="F:dipeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080054">
        <property type="term" value="F:low-affinity nitrate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042937">
        <property type="term" value="F:tripeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17417">
        <property type="entry name" value="MFS_NPF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000147">
        <property type="entry name" value="Protein NRT1/ PTR family 5.10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044739">
        <property type="entry name" value="NRT1/PTR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000109">
        <property type="entry name" value="POT_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018456">
        <property type="entry name" value="PTR2_symporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11654">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00854">
        <property type="entry name" value="PTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01022">
        <property type="entry name" value="PTR2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="106"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="149"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="199"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="331"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="370"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="413"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="496"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="541"/>
            <end position="561"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9675949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="572" mass="62562" checksum="A9EFA17EC4FCA4D9" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YT44</accession>
    <name>A0AA38YT44_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_024831</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C48 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9676032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YT44"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.395.10">
        <property type="entry name" value="Adenoviral Proteinase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="4">
        <location>
            <begin position="193"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9676032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="309" mass="35352" checksum="9F4EE8C3DD4819AD" modified="2024-01-24" version="1">MAKSKESSKQYSLQKGAVPKVDGGNLMEMDKTFEQLKTHLIDMAYGSSSSSCDQILANFEENYTTVKGLFLRSSGKPLKMHEEGTKNDILSCERSLQDTKNMNVNEHYLHTLSNEKVCTEDLNEKNEDACATSIEVHMIPDENENIFPLPIKRSRRDYGKEPLRALCGYPDHVMKTRTSRERKPSKFKVSPFVHNLRKMVKREASEVEFCQTFFKLSDIGKDVFQFSIDWAPSIPTQENGWDCGVHVIRHMQRFKNGDSMTSSDFCNSVKIRREIACDLVLHEGNREKHTIVAIVCTKTSTRAMKKLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YUV0</accession>
    <name>A0AA38YUV0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PVL29_022225</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YUV0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000084">
        <property type="entry name" value="Wall-associated receptor kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000043">
        <property type="entry name" value="Wall-associated receptor kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013695">
        <property type="entry name" value="WAK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045274">
        <property type="entry name" value="WAK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025287">
        <property type="entry name" value="WAK_GUB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27005:SF515">
        <property type="entry name" value="WALL-ASSOCIATED RECEPTOR KINASE-LIKE 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27005">
        <property type="entry name" value="WALL-ASSOCIATED RECEPTOR KINASE-LIKE 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13947">
        <property type="entry name" value="GUB_WAK_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08488">
        <property type="entry name" value="WAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 16" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041266730" description="Protein kinase domain-containing protein" evidence="16">
        <location>
            <begin position="21"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="325"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="399"/>
            <end position="674"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ9677118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="728" mass="81738" checksum="44E2DB6DAE125192" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YX84</accession>
    <name>A0AA38YX84_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 13">Phenylalanine ammonia-lyase</fullName>
            <ecNumber evidence="6 13">4.3.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_020515</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This is a key enzyme of plant metabolism catalyzing the first reaction in the biosynthesis from L-phenylalanine of a wide variety of natural products based on the phenylpropane skeleton.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 13">
            <text>L-phenylalanine = (E)-cinnamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:21384"/>
            <dbReference type="ChEBI" id="CHEBI:15669"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="EC" id="4.3.1.24"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 13">Phenylpropanoid metabolism; trans-cinnamate biosynthesis; trans-cinnamate from L-phenylalanine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the PAL/histidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.1.24" evidence="6 13"/>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YX84"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045548">
        <property type="term" value="F:phenylalanine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006559">
        <property type="term" value="P:L-phenylalanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009698">
        <property type="term" value="P:phenylpropanoid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00332">
        <property type="entry name" value="PAL-HAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.274.20:FF:000001">
        <property type="entry name" value="Phenylalanine ammonia-lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.275.10:FF:000009">
        <property type="entry name" value="Phenylalanine ammonia-lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.200.10:FF:000009">
        <property type="entry name" value="Phenylalanine ammonia-lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.200.10">
        <property type="entry name" value="Fumarase/aspartase (Central domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="entry name" value="Fumarase/aspartase (N-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.274.20">
        <property type="entry name" value="Phenylalanine ammonia-lyase 1, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001106">
        <property type="entry name" value="Aromatic_Lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022313">
        <property type="entry name" value="Phe/His_NH3-lyase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005922">
        <property type="entry name" value="Phe_NH3-lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023144">
        <property type="entry name" value="Phe_NH3-lyase_shielding_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01226">
        <property type="entry name" value="phe_am_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10362">
        <property type="entry name" value="HISTIDINE AMMONIA-LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00221">
        <property type="entry name" value="Lyase_aromatic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="L-aspartase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00488">
        <property type="entry name" value="PAL_HISTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-0585">Phenylalanine catabolism</keyword>
    <keyword evidence="8 13" id="KW-0587">Phenylpropanoid metabolism</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9678366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="710" mass="77228" checksum="D3EA7233931F4ED6" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YXW8</accession>
    <name>A0AA38YXW8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Serine/threonine-protein phosphatase 4 regulatory subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_020645</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPP4R2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YXW8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030289">
        <property type="term" value="C:protein phosphatase 4 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015267">
        <property type="entry name" value="PPP4R2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16487">
        <property type="entry name" value="PPP4R2-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16487:SF0">
        <property type="entry name" value="PROTEIN PHOSPHATASE 4 REGULATORY SUBUNIT 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09184">
        <property type="entry name" value="PPP4R2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="162"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="169"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="205"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="231"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9678522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="242" mass="26978" checksum="FACF8028BD97C983" modified="2024-01-24" version="1">MEISSENLQDSTISSNDADQNHDSVVPVLLNHGSTESEQEIPEEEGRGILEVMASTGKFWHDWDKLKNLLSFQLKQVLSEYPEAKMTTDEQNSSLGETYLDLVKRLDEVLLSFIEGPPFTLQRLCEILLDAQSIYPYLSKLALALEKNLLVTSTLTICTDPYPPTLVQKPDEPPEKGSEEPVQHHDSVQNGVEAEPTQGDKDEIMTEVEEAEIEEDMTIDMEALEEIVVGSSETNSTPPTNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YY26</accession>
    <name>A0AA38YY26_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_020708</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YY26"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31951">
        <property type="entry name" value="BIFUNCTIONAL INHIBITOR/LIPID-TRANSFER PROTEIN/SEED STORAGE 2S ALBUMIN SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31951:SF22">
        <property type="entry name" value="ECA1 GAMETOGENESIS RELATED FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041205362" evidence="1">
        <location>
            <begin position="28"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9678612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="141" mass="15430" checksum="73C3F3B8B1CDD19A" modified="2024-01-24" version="1">MASFCKVSIIAFFLLASGAFLSSPALAFQANEQDFSLASEPSSSSDPGIIPPALRHYLFQCLDKLGKESGPLFFARIFIGEKNEIPTTSLQHLVQMGYKCHSAITNAVISRPEFSDNATVFRTRSDELWRLAETISPLPSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YZB6</accession>
    <name>A0AA38YZB6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Alpha/beta hydrolase fold-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_021191</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDXG' lipolytic enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YZB6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050466">
        <property type="entry name" value="Carboxylest/Gibb_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002168">
        <property type="entry name" value="Lipase_GDXG_HIS_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23024">
        <property type="entry name" value="ARYLACETAMIDE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23024:SF546">
        <property type="entry name" value="CARBOXYLESTERASE 120-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01173">
        <property type="entry name" value="LIPASE_GDXG_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="4">
        <location>
            <begin position="70"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9679184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="300" mass="32691" checksum="74883BE1D92D81A2" modified="2024-01-24" version="1">MVNNPDGSVTRLVTLPSTAPSPDHTTHIPVLSKDITVNPDKNLWVRVFLPREARDSTPPAAGAAKKLPLIVYFHGGGFVICSAATTIFHDLCALMAAEIGAVVVSVEYRLAPEHRLPAAYEDGVETLKWIKSSGEAWVSEYADVSRCYLMGSSAGGNLAYFAGLHVADSVADLEPLKIRGMILHHPFFGGIHRSGSEVRLENDGVLPLCSTDLMWELALPEGVDRDHEYSNPMAKNASEHCSKIRRVGCKFLVAGCEGDLLHDRQVEFVEMLKGNGVEVEAVFERGDCHALFGHVKKFMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YZN2</accession>
    <name>A0AA38YZN2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">chitinase</fullName>
            <ecNumber evidence="2">3.2.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_021562</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1-&gt;4)-beta-linkages in chitin and chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.14"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the glycosyl hydrolase 18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.14" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YZN2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008843">
        <property type="term" value="F:endochitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02877">
        <property type="entry name" value="GH18_hevamine_XipI_class_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000015">
        <property type="entry name" value="Acidic endochitinase SE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045321">
        <property type="entry name" value="Cts1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001579">
        <property type="entry name" value="Glyco_hydro_18_chit_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050542">
        <property type="entry name" value="Glycosyl_Hydrlase18_Chitinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45708:SF21">
        <property type="entry name" value="ACIDIC ENDOCHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45708">
        <property type="entry name" value="ENDOCHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01095">
        <property type="entry name" value="GH18_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0146">Chitin degradation</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041387468" description="chitinase" evidence="11">
        <location>
            <begin position="26"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="GH18" evidence="12">
        <location>
            <begin position="26"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9679668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="297" mass="31760" checksum="FAE5A920DAA923B9" modified="2024-01-24" version="1">MARTPQSTPLLISLSVLALLQTSYAGGIAIYWGQNGNEGTLTETCNTGKYSYVNIAFLNKFGNGQTPEINLAGHCNPASNGCASVSTGIRNCQNRGIKVMLSIGGGSGSYSLSSSTDAQNVANYLWNNFLGGQSSSRPLGGAVLDGIDFDIELGSTQHWDDLARALSGFSKRGRKVYLTAAPQCPFPDKFLGTALNTGLFDYVWVQFYNNAPCQYSSGNINNLLNSWNRWTSSINSRIFMGLPASSAAAGSGFIPANVLTSQILPVIKRSPKYGGVMLWSKYYDDQSGYSSAIKSSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YZS1</accession>
    <name>A0AA38YZS1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_021585</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YZS1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007021">
        <property type="entry name" value="DUF659"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008906">
        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32166:SF67">
        <property type="entry name" value="HAT TRANSPOSON SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32166">
        <property type="entry name" value="OSJNBA0013A04.12 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04937">
        <property type="entry name" value="DUF659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF659" evidence="2">
        <location>
            <begin position="70"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="HAT C-terminal dimerisation" evidence="3">
        <location>
            <begin position="441"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9679711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="580" mass="65657" checksum="8491739D5A8A4D53" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z070</accession>
    <name>A0AA38Z070_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Receptor-like serine/threonine-protein kinase</fullName>
            <ecNumber evidence="18">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">PVL29_021519</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="24">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="18"/>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z070"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048544">
        <property type="term" value="P:recognition of pollen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00028">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000384">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000059">
        <property type="entry name" value="S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.90.10.10:FF:000008">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.10">
        <property type="entry name" value="Bulb-type lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000858">
        <property type="entry name" value="S_locus_glycoprot_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024171">
        <property type="entry name" value="SRK-like_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47974">
        <property type="entry name" value="OS07G0415500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47974:SF29">
        <property type="entry name" value="RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00954">
        <property type="entry name" value="S_locus_glycop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000641">
        <property type="entry name" value="SRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 18 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0430">Lectin</keyword>
    <keyword evidence="12 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 18 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 18" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 21" id="KW-0732">Signal</keyword>
    <keyword evidence="4 18" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041301118" description="Receptor-like serine/threonine-protein kinase" evidence="21">
        <location>
            <begin position="26"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="467"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="23">
        <location>
            <begin position="26"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="523"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="552"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRNR" id="PIRNR000641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KAJ9679619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="857" mass="94149" checksum="53B8B95BE107E329" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z0C6</accession>
    <name>A0AA38Z0C6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Helicase MAGATAMA 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_021574</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z0C6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000326">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045055">
        <property type="entry name" value="DNA2/NAM7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045529">
        <property type="entry name" value="DUF6469"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887:SF515">
        <property type="entry name" value="P-LOOP CONTAINING NUCLEOSIDE TRIPHOSPHATE HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20073">
        <property type="entry name" value="DUF6469"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6469" evidence="8">
        <location>
            <begin position="83"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase helicase" evidence="6">
        <location>
            <begin position="250"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase-like C-terminal" evidence="7">
        <location>
            <begin position="682"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="448"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9679694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1096" mass="124151" checksum="3B3EF1D32FE32EC5" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z1C4</accession>
    <name>A0AA38Z1C4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF3475 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_019571</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z1C4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045927">
        <property type="term" value="P:positive regulation of growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021864">
        <property type="entry name" value="DUF3475"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31371">
        <property type="entry name" value="BNAC09G50660D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31371:SF4">
        <property type="entry name" value="DUF668 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11961">
        <property type="entry name" value="DUF3475"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF3475" evidence="1">
        <location>
            <begin position="92"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF11961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9680293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="176" mass="20390" checksum="95D7712A41F624D1" modified="2024-01-24" version="1">MDSILFSGVQTHLFLFYPSDCSSFIIPVPLSYPNKPIPQTTSHTHYPFSIPFTLNTPRFIPYKLSIFTYLFLTHACRDYIPMKLIMPIFFTHVVNLWNGLSDRELDRVTEEILNLLGIRKLVSDDDGYLMGFALAKIIEDLEFVMRSAAKLGKRCTSLRFQHFEYYFGDSIQNDVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z1G5</accession>
    <name>A0AA38Z1G5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">U-box domain-containing protein</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">RING-type E3 ubiquitin transferase PUB</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_019631</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Functions as an E3 ubiquitin ligase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 5">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z1G5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16664">
        <property type="entry name" value="RING-Ubox_PUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058678">
        <property type="entry name" value="ARM_PUB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045185">
        <property type="entry name" value="PUB22/23/24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045210">
        <property type="entry name" value="RING-Ubox_PUB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22849:SF23">
        <property type="entry name" value="U-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22849">
        <property type="entry name" value="WDSAM1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25598">
        <property type="entry name" value="ARM_PUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04564">
        <property type="entry name" value="U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51698">
        <property type="entry name" value="U_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="U-box" evidence="6">
        <location>
            <begin position="2"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU369093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9680367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="411" mass="46486" checksum="A6BD41339787F8CE" modified="2024-01-24" version="1">MDFPVDFRCPISMELMQDPVTISTGVTYERKNIEKWLFSYNKKTCPTTMQRINSFDITPNHTLKRLILAWQNEEAARSCDSRPVRSLKHDELVSLLATMESTPFKVNSLKKLRAIVGLGHEVKDDFMRCGGVEVLVQIIAQILVESSDFVAFTACEEALGVLHQYLSLSEGDEKTIQLLSKPESIKSLAIMLQRGSSEARFYAVKIFQKLVQADYNWKKFVIQDQGFEFFRSLLELVSDDQICTKASASALELLIEILGSSKKIRLKAIEAGAVCILIELLPDSNRSKCEKMLLLLKLICECAEGRLALVEHGLGIAAISKKLLHVSSAATKITVKILWLICSYHPSERVLEEMLIFGSVKKLLALLHIGNRSSTKEKVVKIFKLHRNSWRQYPCLPWELKDYLGLTNQSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z1L8</accession>
    <name>A0AA38Z1L8_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_019585</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z1L8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9680309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="75" mass="7942" checksum="DEF54278DF8B1A2F" modified="2024-01-24" version="1">MLLPQRHDDTLDVSPSSKSVVSILVIDSIDGIILDRPDSCNLFSVNCLYGHGIVQSTTLLNASGSETISANANLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z377</accession>
    <name>A0AA38Z377_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Methenyltetrahydrofolate cyclohydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_020430</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(6R)-5,10-methenyltetrahydrofolate + H2O = (6R)-10-formyltetrahydrofolate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
            <dbReference type="EC" id="3.5.4.9"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NADP(+) = (6R)-5,10-methenyltetrahydrofolate + NADPH</text>
            <dbReference type="Rhea" id="RHEA:22812"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.5.1.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9681558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z377"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004477">
        <property type="term" value="F:methenyltetrahydrofolate cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004488">
        <property type="term" value="F:methylenetetrahydrofolate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009853">
        <property type="term" value="P:photorespiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01080">
        <property type="entry name" value="NAD_bind_m-THF_DH_Cyclohyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10860:FF:000001">
        <property type="entry name" value="Bifunctional protein FolD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000006">
        <property type="entry name" value="Bifunctional protein FolD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01576">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000672">
        <property type="entry name" value="THF_DH/CycHdrlase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020630">
        <property type="entry name" value="THF_DH/CycHdrlase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020867">
        <property type="entry name" value="THF_DH/CycHdrlase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020631">
        <property type="entry name" value="THF_DH/CycHdrlase_NAD-bd_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010783">
        <property type="entry name" value="PRK14186.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48099:SF5">
        <property type="entry name" value="C-1-TETRAHYDROFOLATE SYNTHASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48099">
        <property type="entry name" value="C-1-TETRAHYDROFOLATE SYNTHASE, CYTOPLASMIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00763">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02882">
        <property type="entry name" value="THF_DHG_CYH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00085">
        <property type="entry name" value="THFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00767">
        <property type="entry name" value="THF_DHG_CYH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0601">Photorespiration</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tetrahydrofolate dehydrogenase/cyclohydrolase catalytic" evidence="13">
        <location>
            <begin position="101"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="Tetrahydrofolate dehydrogenase/cyclohydrolase NAD(P)-binding" evidence="14">
        <location>
            <begin position="237"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00036357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00052194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00058319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00061364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9681558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="389" mass="42252" checksum="8593F5B9F5AED6BF" modified="2024-01-24" version="1">MASYMAFTDCSTSAPTRTFLFSRFRPRRGLPSIPPHLLQGLPVAGFRLPSLRITNSPSSFPKGNAFLRNKQGFRHFRPFTVLASVSASEAAISTEASAMVIDGKSIAKEIRHEITTEISRMKDEIGVIPGLAVILVGDRKDSATYVRNKKKACESVGVKSYEVCLPEDSTEEEVLKFISGFNDDPEVHGILVQLPLPSHMNEQNILNAVSIEKDVDGFHPLNIGRLAMRGREPLFVPCTPKGCIELLHRCDIDIKGKQAVVIGRSNIVGMPAALLLLREDATVSIVHSRTKNPEEITSQADIIISAVGQPNMVRGSWIKPGAVIIDVGINPVEDATSPRGYRLVGDVCYEEACKIASAVTPVPGGVGPMTIAMLLSNTLSSAKRMHNFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z6K2</accession>
    <name>A0AA38Z6K2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GRAM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_018966</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GEM family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z6K2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd13222">
        <property type="entry name" value="PH-GRAM_GEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037848">
        <property type="entry name" value="GEM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004182">
        <property type="entry name" value="GRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31969">
        <property type="entry name" value="GEM-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02893">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00568">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GRAM" evidence="3">
        <location>
            <begin position="174"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="26"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9683192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="312" mass="34049" checksum="DF552F6AB142AF90" modified="2024-01-24" version="1">MDSTTKGSQPLYPSLTPTTPEETAHQPLTSLPSSSPQQVNPQEAQPHHQAGPSSSAPPLPPCQEDAEKWGTHIMGPPAVPTCHPDNKKAALWGAAHDQQNNHYHHPYLQYNPVEKPSSSPMESVLQMFNSWGNKAESTANNIWHNLKTGPSVSKAAWGKVNLTAKAITGGGFESLYKQTFATQPSEKLKKSFACYLSTSTGPVAGTLYLSNIHVAFCSDRPLSFTAPSGQETWSYYKVMVPLSKIGTINPVIMRENPSEKENPSERYIQTVTVDGHDFWFMGFVNYDKASQHLSESMANFVATGIAVQPPVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z6N9</accession>
    <name>A0AA38Z6N9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Polygalacturonase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_019133</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the glycosyl hydrolase 28 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z6N9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004650">
        <property type="term" value="F:polygalacturonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000019">
        <property type="entry name" value="polygalacturonase At1g48100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000743">
        <property type="entry name" value="Glyco_hydro_28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31375">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00295">
        <property type="entry name" value="Glyco_hydro_28"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0134">Cell wall</keyword>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6 8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041457401" description="Polygalacturonase" evidence="9">
        <location>
            <begin position="28"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9683418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="479" mass="51215" checksum="CD7E7842E3E291EE" modified="2024-01-24" version="1">MSGFKRLALTVLIALLACSSSFQACSARKAKYTRQLKTVGEGYDGYNYRDPKLMDEGATPLKNAGSSTFNVLHFGAKGDGKADDTKAFGAAWAAACQVRGSTMMVPSGSVFLVKPIIFSGPNCEPNIVFQLDGKIIAPRSSSAWGSGIYQWLDFSKLQGITIRGKGTIDGQGSIWWNDWTDESESETPTAGDSLYSSKQDSISSELSAKMPDTKPTALRFFGSSLVTVTGITIQNSPLIHLKFDDCTGVQVSGVTVSSPGDSPNTDGIHLQNTQNVVIYSTNLACGDDCVSIQTGCSNVFVHNVHCGPGHGISIGGLGRDNTKACVKNVTVRDVTMQDTLTGVRIKTWQGGSGSVSGVMFSNIQVSNVKTPIMIDQFYCDKYRCQNETKAVAVSNINYVNIKGTYTGKPVHFACSDSLPCSGVTLTTIQLRPAQEGALSYAPFCWETYGQLKTNTIPPIDCLQTGKPSKNQLHANGDSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z7I6</accession>
    <name>A0AA38Z7I6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DUF2428 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_019305</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the THADA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z7I6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056842">
        <property type="entry name" value="THADA-like_TPR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019442">
        <property type="entry name" value="THADA/TRM732_DUF2428"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051954">
        <property type="entry name" value="tRNA_methyltransferase_THADA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14387:SF0">
        <property type="entry name" value="DUF2428 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14387">
        <property type="entry name" value="THADA/DEATH RECEPTOR INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10350">
        <property type="entry name" value="DUF2428"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25151">
        <property type="entry name" value="TPR_Trm732_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="DUF2428" evidence="3">
        <location>
            <begin position="13"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA (32-2'-O)-methyltransferase regulator THADA-like C-terminal TPR repeats region" evidence="4">
        <location>
            <begin position="307"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9683672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1141" mass="128021" checksum="BD72E5DCF8017044" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z7Q0</accession>
    <name>A0AA38Z7Q0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HMA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_016449</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9683958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z7Q0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22814">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN ATOX1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22814:SF304">
        <property type="entry name" value="HEAVY METAL TRANSPORT_DETOXIFICATION SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMA" evidence="2">
        <location>
            <begin position="2"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9683958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="71" mass="8066" checksum="CE9AA3857028F06A" modified="2024-01-24" version="1">MANVVELKVGLHCEECIKKILKAIKKIEADIETYNIDTQLNKVIVTGNVTEEEVIRVLQKIGKRASNWQSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z927</accession>
    <name>A0AA38Z927_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aldehyde dehydrogenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_016805</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z927"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11699">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="1">
        <location>
            <begin position="36"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9684512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="85" mass="9067" checksum="EF9EF2273DC287C8" modified="2024-01-24" version="1">MRSQSSRFSTAVATDKGQITPPVQIVYTQLLINGRFVDAASGKTFPTLDPRTGEVIANVAEGEAADINRAVSAARKAFDDGSWPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZBA3</accession>
    <name>A0AA38ZBA3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Disease resistance N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_017781</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZBA3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="4">
        <location>
            <begin position="77"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9685845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="145" mass="16004" checksum="106078A57C36BE32" modified="2024-01-24" version="1">MITLALITLTPLYPKSRGYLTADITLLPDTIKAPLRPKGKNLEVISPLVDKASTDALLSASLQVLFDRLASPDLINFIGKLLVVHKALNDAEMKQFSDPLVKDWLVQVKDVVYHAEDLLVEIATETLRCQIEAAEHPQTGGIYQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZCE2</accession>
    <name>A0AA38ZCE2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycine-rich cell wall structural protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_015377</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZCE2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01228">
        <property type="entry name" value="EGGSHELL"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041214349" description="Glycine-rich cell wall structural protein 1" evidence="1">
        <location>
            <begin position="27"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9686448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="328" mass="24495" checksum="8D62145CD926C5C8" modified="2024-01-24" version="1">MGASPKWLCVILFLSVVFHMSAIALGDDKVDKTRFRDDGCRFGGQRGCGGGRGGGGGGGFGGGGGGGVGGGSGKGGGFGAGGGVGGGAGGGVGGGGGFGGGGGGGVGGGSGHGGGFGAGGGVGGGAGGGVGGGGGGGGGGGGGAGGGSGHGGGFGAGGGVGGGAGGGVGGGGGGGGGGGGGGGGAGGGSGHGGGFGAGGGVGVGGGAGGAGGGGGGGGGGGGGAGGGSGHGGGFGAGGGVGSGAGGGVGGGGGVGGGSGGGVGGGSGHGGGFGAGGGAGGGAGGGVGGGHGVGGGGGFGIGIGIGVGVGTGAGRGSGSGSGSGGGGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZDP0</accession>
    <name>A0AA38ZDP0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7815 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_015398</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZDP0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056717">
        <property type="entry name" value="DUF7815"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36308">
        <property type="entry name" value="DENTIN SIALOPHOSPHOPROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36308:SF1">
        <property type="entry name" value="DENTIN SIALOPHOSPHOPROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25122">
        <property type="entry name" value="DUF7815"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7815" evidence="2">
        <location>
            <begin position="53"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="375"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="429"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="377"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="429"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="455"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9686474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="514" mass="56284" checksum="DA08F9F9348856E0" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZDX0</accession>
    <name>A0AA38ZDX0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">beta-ketoacyl-[acyl-carrier-protein] synthase I</fullName>
            <ecNumber evidence="1">2.3.1.41</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_015460</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.41" evidence="1"/>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZDX0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00834">
        <property type="entry name" value="KAS_I_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000024">
        <property type="entry name" value="3-oxoacyl-[acyl-carrier-protein] synthase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000794">
        <property type="entry name" value="Beta-ketoacyl_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018201">
        <property type="entry name" value="Ketoacyl_synth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11712:SF297">
        <property type="entry name" value="3-OXOACYL-[ACYL-CARRIER-PROTEIN] SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11712">
        <property type="entry name" value="POLYKETIDE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00825">
        <property type="entry name" value="PKS_KS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00606">
        <property type="entry name" value="KS3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52004">
        <property type="entry name" value="KS3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ketosynthase family 3 (KS3)" evidence="3">
        <location>
            <begin position="28"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS52004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9686594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="339" mass="36621" checksum="34BEE254DAE5FC65" modified="2024-01-24" version="1">MAKLCSRMFFTARLQFNRFISSSTTAFPRRVVVTGLGMVTPLGCGVETTWKRLIEGKCAIRAISPEDLKMNGFDRETQLRTYDQLTSKVAAIVPCGTNPGDFNEELWLNSRDSRSIARFIGYALCAADEALSDAQWAPSEQEQKEMTGVSIGGGTGSISDILDAAQMICEKRLRRLSPFFIPRILINMASGHVSMRYGFQGPNHAAVTACATGAHSIGDAVRMIQFGDSDVMVAGGTESSIDALSIAGFCRSRALTTKYNSCPEEASRPFDCGRDGFVIGEGSGILVLEELVHAKKRGAKIYAEVQGYGMSGCFSTCVNINIFMAMLRLLIGSAVIGIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZEB8</accession>
    <name>A0AA38ZEB8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="ORF">PVL29_015678</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="26">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="26">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZEB8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000015">
        <property type="entry name" value="Somatic embryogenesis receptor kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000024">
        <property type="entry name" value="Somatic embryogenesis receptor kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000016">
        <property type="entry name" value="Somatic embryogenesis receptor-like kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47988">
        <property type="entry name" value="SOMATIC EMBRYOGENESIS RECEPTOR KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="16 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-0675">Receptor</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10 24" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 23" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="24">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041377930" description="non-specific serine/threonine protein kinase" evidence="24">
        <location>
            <begin position="26"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="225"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="25">
        <location>
            <begin position="288"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="591"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="316"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EMBL" id="KAJ9686923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="610" mass="67071" checksum="105E323218CCB068" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZFH0</accession>
    <name>A0AA38ZFH0_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_016215</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZFH0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057135">
        <property type="entry name" value="At4g27190-like_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050905">
        <property type="entry name" value="Plant_NBS-LRR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463:SF179">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23247">
        <property type="entry name" value="LRR_RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="6">
        <location>
            <begin position="571"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein At4g27190-like leucine-rich repeats" evidence="5">
        <location>
            <begin position="788"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9687642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1012" mass="116702" checksum="1AAC4DFB07709B37" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZHT0</accession>
    <name>A0AA38ZHT0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">PVL29_014619</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="24">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9689059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZHT0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099402">
        <property type="term" value="P:plant organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000309">
        <property type="entry name" value="Leucine-rich repeat receptor protein kinase MSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000221">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase PXL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000445">
        <property type="entry name" value="MDIS1-interacting receptor like kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000400">
        <property type="entry name" value="Nuclear pore complex protein NUP107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001390">
        <property type="entry name" value="Probable leucine-rich repeat receptor-like protein kinase At1g35710"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001375">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051716">
        <property type="entry name" value="Plant_RL_S/T_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48053">
        <property type="entry name" value="LEUCINE RICH REPEAT FAMILY PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48053:SF168">
        <property type="entry name" value="LRR RECEPTOR-LIKE KINASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="15 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9 22" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041372478" description="non-specific serine/threonine protein kinase" evidence="22">
        <location>
            <begin position="22"/>
            <end position="1208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="854"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="919"/>
            <end position="1186"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="947"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KAJ9689059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1208" mass="132283" checksum="5C9612C2418EB2F9" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZIQ8</accession>
    <name>A0AA38ZIQ8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 20">Peroxidase</fullName>
            <ecNumber evidence="4 20">1.11.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">PVL29_012344</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="22">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 20">
            <text>2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:56136"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:139520"/>
            <dbReference type="ChEBI" id="CHEBI:139521"/>
            <dbReference type="EC" id="1.11.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17 20">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="17 20">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17 20">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="17 20">Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="20">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peroxidase family. Ascorbate peroxidase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="20">Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.7" evidence="4 20"/>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9689600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZIQ8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140825">
        <property type="term" value="F:lactoperoxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00693">
        <property type="entry name" value="secretory_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.420.10:FF:000008">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.520.10:FF:000001">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.420.10">
        <property type="entry name" value="Peroxidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000823">
        <property type="entry name" value="Peroxidase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019794">
        <property type="entry name" value="Peroxidases_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019793">
        <property type="entry name" value="Peroxidases_heam-ligand_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033905">
        <property type="entry name" value="Secretory_peroxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31235">
        <property type="entry name" value="PEROXIDASE 25-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00458">
        <property type="entry name" value="PEROXIDASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00461">
        <property type="entry name" value="PLPEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00435">
        <property type="entry name" value="PEROXIDASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00436">
        <property type="entry name" value="PEROXIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50873">
        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 17 20" id="KW-0106">Calcium</keyword>
    <keyword evidence="13 19" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 20" id="KW-0349">Heme</keyword>
    <keyword evidence="15 20" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="12 17 20" id="KW-0408">Iron</keyword>
    <keyword evidence="8 17 20" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 20" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6 20" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 20" id="KW-0964">Secreted</keyword>
    <keyword evidence="9 20" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041488565" description="Peroxidase" evidence="20">
        <location>
            <begin position="27"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="Plant heme peroxidase family profile" evidence="21">
        <location>
            <begin position="28"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="16">
        <location>
            <position position="69"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="17">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="18">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="38"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="71"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="122"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="201"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU362060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAJ9689600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="326" mass="35371" checksum="1AE9E4B936188F96" modified="2024-01-24" version="1">MVASKGFYCLFLQLLLLSFVFDVANSQGLKVGFYGKTCPNAEAIVKKVVDQTMSVAPSLSGPLLRMHFHDCFVRGCEGSVLLNSSTEQAEKNAFPNLSLRGYQVIDRVKSALEKECPGVVSCSDILALVARDVVVVMEGPSWEVETGRRDGRVSNITEALTNLIPPTANITQLKSGFQQKGLSVKDLVVLSGGHTLGTSHCSSFSSRLYNFTGKGDTDPDLDPNYIAKLKNKCKQGDANSLVEMDPGSFKTFDESYYTLVGKRRGLFVSDAALLDDSETKAYVKLQATTHGSTFFEDFGVSMIKMGRIGVLTGTSGEIRKECALVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZKT2</accession>
    <name>A0AA38ZKT2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PVL29_012722</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZKT2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048544">
        <property type="term" value="P:recognition of pollen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00028">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01098">
        <property type="entry name" value="PAN_AP_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.90.10.10:FF:000029">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000195">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000467">
        <property type="entry name" value="Liguleless narrow1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.10">
        <property type="entry name" value="Bulb-type lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000858">
        <property type="entry name" value="S_locus_glycoprot_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002:SF1095">
        <property type="entry name" value="G-TYPE LECTIN S-RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE RKS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002">
        <property type="entry name" value="RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD1-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08276">
        <property type="entry name" value="PAN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00954">
        <property type="entry name" value="S_locus_glycop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00473">
        <property type="entry name" value="PAN_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="591"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="17">
        <location>
            <begin position="177"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="18">
        <location>
            <begin position="492"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="671"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="699"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ9690208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="985" mass="110467" checksum="3590F5AD7511D7B2" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZL18</accession>
    <name>A0AA38ZL18_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Terpene cyclase/mutase family member</fullName>
            <ecNumber evidence="4">5.4.99.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_013271</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the terpene cyclase/mutase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.-" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZL18"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042300">
        <property type="term" value="F:beta-amyrin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016104">
        <property type="term" value="P:triterpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02892">
        <property type="entry name" value="SQCY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.20:FF:000002">
        <property type="entry name" value="Terpene cyclase/mutase family member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.20:FF:000022">
        <property type="entry name" value="Terpene cyclase/mutase family member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032696">
        <property type="entry name" value="SQ_cyclase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032697">
        <property type="entry name" value="SQ_cyclase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018333">
        <property type="entry name" value="Squalene_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002365">
        <property type="entry name" value="Terpene_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01787">
        <property type="entry name" value="squalene_cyclas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11764:SF58">
        <property type="entry name" value="BETA-AMYRIN SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11764">
        <property type="entry name" value="TERPENE CYCLASE/MUTASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13243">
        <property type="entry name" value="SQHop_cyclase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13249">
        <property type="entry name" value="SQHop_cyclase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01016">
        <property type="entry name" value="Prenyltransferase_Like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01074">
        <property type="entry name" value="TERPENE_SYNTHASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Squalene cyclase N-terminal" evidence="6">
        <location>
            <begin position="99"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="Squalene cyclase C-terminal" evidence="5">
        <location>
            <begin position="412"/>
            <end position="750"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9691020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="759" mass="87308" checksum="DCB40C5D72E76871" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZM34</accession>
    <name>A0AA38ZM34_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_013141</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZM34"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF78">
        <property type="entry name" value="OS12G0587133 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041328725" evidence="1">
        <location>
            <begin position="17"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9690838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="117" mass="13645" checksum="1AE01E4CCDECBBAB" modified="2024-01-24" version="1">MTFLCWLLMWFEAIFGLRVILDKSEPIPIGRVENVEELVSELGCKVGRLSFTYLGMSLGASFKVVATWDRIEERFHKRLAMWKQQYISKGWENHLDSKHFVQPTYLFHVYSSIAKGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZN56</accession>
    <name>A0AA38ZN56_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PVL29_011301</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000129">
        <property type="entry name" value="Leucine-rich repeat receptor-like kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000394">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000146">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase IOS1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000039">
        <property type="entry name" value="receptor-like protein kinase FERONIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024788">
        <property type="entry name" value="Malectin-like_Carb-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45631">
        <property type="entry name" value="OS07G0107800 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45631:SF206">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12819">
        <property type="entry name" value="Malectin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="14 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="443"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1028"/>
            <end position="1050"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
            <begin position="495"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
            <begin position="694"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
            <begin position="1080"/>
            <end position="1359"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="722"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="1108"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAJ9692161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1359" mass="150546" checksum="0F4AF5CF3AD0F0AE" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZNN5</accession>
    <name>A0AA38ZNN5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">VHS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_011393</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Clathrin-coated vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">trans-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZNN5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032588">
        <property type="term" value="C:trans-Golgi network membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03572">
        <property type="entry name" value="ENTH_like_Tepsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035802">
        <property type="entry name" value="ENTH/VHS_tepsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039273">
        <property type="entry name" value="TEPSIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002014">
        <property type="entry name" value="VHS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21514">
        <property type="entry name" value="AP-4 COMPLEX ACCESSORY SUBUNIT TEPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21514:SF0">
        <property type="entry name" value="AP-4 COMPLEX ACCESSORY SUBUNIT TEPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01417">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00288">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50179">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VHS" evidence="6">
        <location>
            <begin position="20"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="137"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="186"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="195"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="223"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="250"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9692312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="709" mass="77347" checksum="FC119AC21492FF47" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZSH7</accession>
    <name>A0AA38ZSH7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycine-rich protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_010088</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZSH7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36040">
        <property type="entry name" value="OS04G0188500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36040:SF5">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041309389" description="Glycine-rich protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9694426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="118" mass="12642" checksum="F7EDE01A8663812D" modified="2024-01-24" version="1">MKIIAVLFILMLCCRLAASGGGGRDEMDGEEQGKHVDEKESRKVGGYPESNHVDNHHGIPRQYYHDWGSSPVGGDGGSNVGGGGGGSDNAQEVMGNQLLLIWLSFESNEEFWCFGAAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZTG1</accession>
    <name>A0AA38ZTG1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_010450</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HMGB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZTG1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031061">
        <property type="entry name" value="HMGB_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46261:SF1">
        <property type="entry name" value="HIGH MOBILITY GROUP B PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46261">
        <property type="entry name" value="HIGH MOBILITY GROUP B PROTEIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="7">
        <location>
            <begin position="56"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="5">
        <location>
            <begin position="56"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="123"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="10"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="33"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9694968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="178" mass="20012" checksum="C67D34BE28F640CE" modified="2024-01-24" version="1">MKAARGKGAARKDTKEALKPAEDRKVGKRKAAVKAEKSSKRATTKDKKAKKDPNKPKRPPSAFFVFLEEFRKVFKKENPNVKAVSAVGKAGGEKWKSLSEAEKAPYEAKAAKKKAEYEKIMNAYNKKQESTADDGDEESDRSKSEVNDQDDEESAEVMKLIIWVELTIGFFMLLHMLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZTG5</accession>
    <name>A0AA38ZTG5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Expansin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_010269</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Causes loosening and extension of plant cell walls by disrupting non-covalent bonding between cellulose microfibrils and matrix glucans. No enzymatic activity has been found.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Secreted</location>
            <location evidence="7">Cell wall</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Membrane</location>
            <topology evidence="7">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the expansin family. Expansin A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZTG5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009664">
        <property type="term" value="P:plant-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22274">
        <property type="entry name" value="DPBB_EXPA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.10:FF:000001">
        <property type="entry name" value="Expansin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.760:FF:000001">
        <property type="entry name" value="Expansin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.760">
        <property type="entry name" value="Expansin, cellulose-binding-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007118">
        <property type="entry name" value="Expan_Lol_pI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002963">
        <property type="entry name" value="Expansin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007112">
        <property type="entry name" value="Expansin/allergen_DPBB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007117">
        <property type="entry name" value="Expansin_CBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036749">
        <property type="entry name" value="Expansin_CBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009009">
        <property type="entry name" value="RlpA-like_DPBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31867">
        <property type="entry name" value="EXPANSIN-A15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03330">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01357">
        <property type="entry name" value="Expansin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01226">
        <property type="entry name" value="EXPANSIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01225">
        <property type="entry name" value="EXPANSNFAMLY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00837">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49590">
        <property type="entry name" value="PHL pollen allergen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50843">
        <property type="entry name" value="EXPANSIN_CBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50842">
        <property type="entry name" value="EXPANSIN_EG45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0134">Cell wall</keyword>
    <keyword evidence="6 7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041483237" description="Expansin" evidence="7">
        <location>
            <begin position="22"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like EG45" evidence="8">
        <location>
            <begin position="50"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like CBD" evidence="9">
        <location>
            <begin position="174"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9694714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="258" mass="27649" checksum="F4D483B29FD8128F" modified="2024-01-24" version="1">MALLTLLSLASLLSFLTLVHARIPGVFTGGPWESAHATFYGGSDASGTMGGACGYGNLYSQGYGVNTAALSTALFNSGLSCGACFEIKCANDPKWCHSGSPSIFVTGTNFCPPNFALPSDNGGWCNPPRPHFDLAMPMFLKIAEYRAGIVPVAFRRVPCRKSGGIRFTVNGFRYFNLVLVTNVAGAGDIVRVSVKGSKTGWMSMSRNWGQNWQSNAVLVGQSLSFRVTGSDRRTSTSLNVAPANWQFGQTFSGRNFRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZV18</accession>
    <name>A0AA38ZV18_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_010973</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the AP2/ERF transcription factor family. ERF subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZV18"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31241">
        <property type="entry name" value="DEHYDRATION-RESPONSIVE ELEMENT-BINDING PROTEIN 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31241:SF2">
        <property type="entry name" value="DEHYDRATION-RESPONSIVE ELEMENT-BINDING PROTEIN 2F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="8">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="7"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9695761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="241" mass="27191" checksum="DCC9CCE66F5A64BD" modified="2024-01-24" version="1">MENNRKSPLKQWKKGPTRGKGGPQNATCEYCGNRTRLWLGSLTTAEKATMACDEAARILYGPDAYLNLPHLQCNFNPPIKSQNLKWFLSKNFVSMFPSSGLFNINAQTSVHLIHQRLEDLKKNGVIDPLLLVLLPVIQKLIHGTNEKDMESSPEQIPGEHEEKPRIDLNEFLQQLGMPRDECQSEVSNMTESLAAPESSLKDDNELVGFVEEKDHQGADASNFQGYGMYEEPAYPPYIWNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZW76</accession>
    <name>A0AA38ZW76_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_008536</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZW76"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35726">
        <property type="entry name" value="GLUTAMIC ACID-RICH PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35726:SF4">
        <property type="entry name" value="GLUTAMIC ACID-RICH PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9696361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="140" mass="15365" checksum="8BD076D36F9FDF1F" modified="2024-01-24" version="1">MESRKLMDVSSFLLFEASGDSEQLESDPGMAVSEHGIEVDEDDAESCSCDLSESVVQVEEFDGFDAEGYDDSCDVEEEEEEEEEEDGNDQTWTSKPVGPQRLAVGLTPGDRKSSVCADSTEGKSKETEKSRLFWEACLAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZYN0</accession>
    <name>A0AA38ZYN0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ADP-ribosylation factor-related protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_009237</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZYN0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034067">
        <property type="term" value="P:protein localization to Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04160">
        <property type="entry name" value="Arfrp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000914">
        <property type="entry name" value="ADP-ribosylation factor-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45909">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45909:SF1">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="6 7 9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0519">Myristate</keyword>
    <keyword evidence="3 7 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="24"/>
            <end position="31"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="133"/>
            <end position="136"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9697340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="205" mass="23121" checksum="E4553632D6548BAB" modified="2024-01-24" version="1">MFSLFHGLWKYLFSKAEFHVLILGIDKAGKTTLLEKLKGLYTNLEGLPPDRIVPTVGLNIGRVELSNTKLVFWDLGGQPSLRSIWEKYYEEAHAVVFVIDASCPSRFEDSKSALEKVIRHEDLQGAPLLILANKQDLDNAASSDELARYLDLKNLDERIYMFEAVSTLDGMGIKESMNWLVEVMERSKRTEMLRVRAGVTSPASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZYR2</accession>
    <name>A0AA38ZYR2_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_009190</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZYR2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098554">
        <property type="term" value="C:cytoplasmic side of endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030660">
        <property type="term" value="C:Golgi-associated vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098553">
        <property type="term" value="C:lumenal side of endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042500">
        <property type="term" value="F:aspartic endopeptidase activity, intramembrane cleaving"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033619">
        <property type="term" value="P:membrane protein proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007369">
        <property type="entry name" value="Peptidase_A22B_SPP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12174">
        <property type="entry name" value="SIGNAL PEPTIDE PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12174:SF22">
        <property type="entry name" value="SIGNAL PEPTIDE PEPTIDASE-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04258">
        <property type="entry name" value="Peptidase_A22B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="28"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="121"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="163"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9697287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="209" mass="23313" checksum="1E20B3866B83A9AA" modified="2024-01-24" version="1">MHLKLQLGLLDPFMSHCYSKHWILNNLLGISICIAFVSHVCLWNIKICAMLLVLFSKRFFRANVMVLVVVQQASNPVHTIANSLSLLKLQLITKKLELLVKIVFSRNLFGVVPSGNSADFMMLDLSDMAIPAMLLALVLCFNHRKSRDSISPLDIPLAKGYKYIWYAILGYAIGLVTALATSVLTHHQQNLPLCTLGPHSCMTTFGMQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZZC5</accession>
    <name>A0AA38ZZC5_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="22">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">PVL29_009434</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="22">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZZC5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23509">
        <property type="entry name" value="Gnk2-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000129">
        <property type="entry name" value="cysteine-rich receptor-like protein kinase 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000142">
        <property type="entry name" value="Cysteine-rich receptor-like protein kinase 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.430.20">
        <property type="entry name" value="Gnk2 domain, C-X8-C-X2-C motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002902">
        <property type="entry name" value="GNK2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038408">
        <property type="entry name" value="GNK2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002:SF1104">
        <property type="entry name" value="CYSTEINE-RICH RECEPTOR-LIKE PROTEIN KINASE 27-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002">
        <property type="entry name" value="RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD1-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01657">
        <property type="entry name" value="Stress-antifung"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51473">
        <property type="entry name" value="GNK2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 19" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041235704" evidence="19">
        <location>
            <begin position="26"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="261"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="21">
        <location>
            <begin position="29"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="21">
        <location>
            <begin position="133"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
            <begin position="319"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="230"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="232"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="347"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAJ9697602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="648" mass="71982" checksum="9EDA2D6A58B5F958" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A5F1</accession>
    <name>A0AA39A5F1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">CDC20/Fizzy WD40 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_006430</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin-protein ligase complex that controls progression through mitosis and the G1 phase of the cell cycle.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat CDC20/Fizzy family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A5F1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010997">
        <property type="term" value="F:anaphase-promoting complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990757">
        <property type="term" value="F:ubiquitin ligase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905786">
        <property type="term" value="P:positive regulation of anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033010">
        <property type="entry name" value="Cdc20/Fizzy"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056150">
        <property type="entry name" value="WD40_CDC20-Fz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918">
        <property type="entry name" value="CELL DIVISION CYCLE 20 CDC20 FIZZY -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918:SF8">
        <property type="entry name" value="FI02843P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24807">
        <property type="entry name" value="WD40_CDC20-Fz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 8" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="CDC20/Fizzy WD40" evidence="10">
        <location>
            <begin position="130"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="176"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="260"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="396"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="436"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF24807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9701074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="455" mass="50820" checksum="31331128AB5A858C" modified="2024-01-24" version="1">MADPLSVILLARLSRGSLNSCPQVQFLQRWENLDRFIPNRSAMDFDFAHYMLTQRGKGKENQSAVRSQSKEAYLKLLAETFNMNRSRILTFKNKPPTPVKLIPDEFYSSVHQSKPSKPLRRIPQTPERTLDAPDIIDDFCLNLMDWGSSNVLALALQNTVYLWDASNGSASELVTVDDENGPVTSVSWAADGQYIAIGLNSSDVQLWDSTANRLLRTLRGGHQSRVGSLDWKNHILTTGGMDGQIINNDVRVHSHIVATFRGHRQEVCGLKWSTSGQQLASGGNDNLLYIWDRSMASMHSRSQWLHRLEDHTAAVKALAWCPFQRNLLASGGGGSDCCIKFWNTHTGACLNSVDTGSQVCALLWNKNERELLSSHGFMQNQMTLWMYPSMVKIADLTGHTSRVLFMAQSPDGRTVATAAGDETLKFWNAFGTPQVKKAAPKAEHPGPFPHIRRIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A5K6</accession>
    <name>A0AA39A5K6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">PGG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_006649</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A5K6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026961">
        <property type="entry name" value="PGG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24186:SF53">
        <property type="entry name" value="PGG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24186">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13962">
        <property type="entry name" value="PGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="485"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="523"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="558"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="590"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="125"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="207"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="275"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="311"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="PGG" evidence="10">
        <location>
            <begin position="476"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="21"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9701391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="615" mass="68073" checksum="D1738DDF53B0B652" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A7Y1</accession>
    <name>A0AA39A7Y1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Golgi SNAP receptor complex member 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_004399</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport. It belongs to a super-family of proteins called t-SNAREs or soluble NSF (N-ethylmaleimide-sensitive factor) attachment protein receptor.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of several multiprotein Golgi SNARE complexes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the GOSR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A7Y1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005801">
        <property type="term" value="C:cis-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005797">
        <property type="term" value="C:Golgi medial cisterna"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048219">
        <property type="term" value="P:inter-Golgi cisterna vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023601">
        <property type="entry name" value="Golgi_SNAP_su1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21094:SF0">
        <property type="entry name" value="GOLGI SNAP RECEPTOR COMPLEX MEMBER 1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21094">
        <property type="entry name" value="GOS-28 SNARE- RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12352">
        <property type="entry name" value="V-SNARE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF027109">
        <property type="entry name" value="Golgi_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="7 9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="199"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR027109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9702645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="220" mass="24927" checksum="2E0C153F7C49D84F" modified="2024-01-24" version="1">MDPPSSWDALRKQARKLEAQLDEQMHLYRKLVSMKVDGDKEKELDSGIDQLLKQLQQVNSHMQAWVSSGGSEIFSHTLTRHQEILQDLTQEFYRLRSSFRAKKEHASLLEDFREFDRSRLDLEEGGGSEQALLKEHASISRSTGQMDTVISQAQATLGALVFQRSTFGGINSKLSNVSSRLPTVNNILSAIKRKKSMDTIILSLVASVCTFLILIYWLTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AAW2</accession>
    <name>A0AA39AAW2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">DEAD-box ATP-dependent RNA helicase 21</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PVL29_002665</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the DEAD box helicase family. DDX23/PRP28 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AAW2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17945">
        <property type="entry name" value="DEADc_DDX23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000322">
        <property type="entry name" value="probable ATP-dependent RNA helicase DDX23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000520">
        <property type="entry name" value="probable ATP-dependent RNA helicase DDX23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 15" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="4 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="19">
        <location>
            <begin position="27"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="17">
        <location>
            <begin position="58"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="18">
        <location>
            <begin position="280"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="415"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="14">
        <location>
            <begin position="27"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="436"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00068856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAJ9704196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="447" mass="50079" checksum="E91FEB4656C8ABB4" modified="2024-01-24" version="1">MTERDWRIFREDFNISYKGSKIPRPMRSWVESKLSTELLKAVERAGYKTPSPIQMAAIPLGLQQRDVIGIAETGSGKTAAFVLPMLTYISRLPPMSEENEAEGPYAVVMAPTRELAQQIEDETVKFAHYLGIKVVSIVGGQSIEEQGFRIRQGCEVVIATPGRLIDCLERRYAVLNQCNYVVLDEADRMIDMGFEPQVVGVLDAMPSSNLKPENEDEELDEKKIYRTTYMFSATMPPAVERLARKYLRNPVVVTIGTAGKATDLITQHVIMMKESEKMFKLQKLLDELGDKTAIVFINTKKSADNLAKGLDKAGYRVTTLHGGKSQEQREISLEGFRTKRYNVLVATDVAGRGIDIPDVAHVINYDMPGNIEMYTHRIGRTGRAGKTGVATTFLTLHDSDVFYDLKQMLIQNNSPVPPELARHEASKFKPGAIPDRPPRRNDTVFAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AC67</accession>
    <name>A0AA39AC67_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_003053</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AC67"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024489">
        <property type="entry name" value="Organ_specific_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33731:SF2">
        <property type="entry name" value="ORGAN-SPECIFIC PROTEIN S2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33731">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10950">
        <property type="entry name" value="Organ_specific"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041293810" evidence="2">
        <location>
            <begin position="22"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9704821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="116" mass="13270" checksum="A9AAB2BB7EE2FA85" modified="2024-01-24" version="1">MAYSLAFFTIFSLLLVGNATGGRKEPGVYWRDVMKDEPMPKKPNCQAIPGARNGGDIVKGFEPKKEKVFWVYDDEDAKLTEEKSFVKDLEPRTNKRKEKPFVGHFEPRPNVSAYNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AD25</accession>
    <name>A0AA39AD25_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serine carboxypeptidase-like 18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_003372</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AD25"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019748">
        <property type="term" value="P:secondary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF224">
        <property type="entry name" value="SERINE CARBOXYPEPTIDASE-LIKE 7 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9705286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="335" mass="39230" checksum="FC68F090F4E937AE" modified="2024-01-24" version="1">MIFLDAPVGTGFSYARTVEAYNISDTLLVAQIYTFLRKWLLNHPQFHPNQLYISGDSYSGIIVPLLFRKYLMVSNEAGMEPKMNLKGYTLGNPLTDKLNDINSRVPFAHRVSLLSDELYECIEKIYLPDVLEPKCATLCPRPNASILWDTSSQEENFFYLPYPPPQQDEPWCRDYHYLLSYIWANDRNVQNALHIREMQFESYTYDVFNSIDYHQNLSKKGLRALIYCGDHDMVVPYIGTQEWIASLDLNISDAWKPWFVDGQVAGFRIEYSHNKYQMTYATVKGGGHTAPEYKPKECFAMVYRWFAYYFLYLKSLFNNSPNLYGFCRVKSHYFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AEJ3</accession>
    <name>A0AA39AEJ3_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_003560</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AEJ3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9705560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="116" mass="13323" checksum="5D91751FEF7E862B" modified="2024-01-24" version="1">MAARYRDHRNKCHKHFKKYPTIALAKQNPYKHERMLELQKQPVSEGGQALKEAEICVQVLGSRSGYIKGLGHGPRPPSSSSKSTHKSHREIELENELKATRELLQSQETRIQHQQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AFH7</accession>
    <name>A0AA39AFH7_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_001838</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AFH7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9706561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="30" mass="3544" checksum="2C0304BA96A54FF9" modified="2024-01-24" version="1">MIHVPRKLKAQNLEIVWSGDAWICTKQLQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AFZ9</accession>
    <name>A0AA39AFZ9_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_003686</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AFZ9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044659">
        <property type="entry name" value="PELPK1_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33088:SF105">
        <property type="entry name" value="ESX-1 SECRETION-ASSOCIATED PROTEIN ESPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33088">
        <property type="entry name" value="MUCIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041251508" evidence="2">
        <location>
            <begin position="26"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="57"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="91"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9705718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="156" mass="16293" checksum="0BF8E5A5A2716641" modified="2024-01-24" version="1">MASCNCFILSLFVLLSFSSIDVSLAARRLLDTAPPSVPMTPVLPTLPKPTLPPLPSSLPTLPTLPSLPTLPNPTMPPLPSLPTMPQPTLPSLPTTQPSLPKATLPPLPSMPTMPSIPNLTLPPLPTTLPTIPSIPTIPTTMPSIPFLSPPPTTTSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AG78</accession>
    <name>A0AA39AG78_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phospholipase-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_001814</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AG78"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007942">
        <property type="entry name" value="PLipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35358:SF7">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35358">
        <property type="entry name" value="OS06G0711100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05278">
        <property type="entry name" value="PEARLI-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="165"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9706512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="230" mass="26084" checksum="55E58861EB992142" modified="2024-01-24" version="1">MGNDHLVSPCGHEDWCSGSPSQQQSLIMDVPFLKKVYPSDDITHMGGSNCANDTKTVKVGSYFVNPSLSMTLQAIINKHGDIAKDCQLESDYMRTPVLEGICKVVQDLQNIQFSELKQHHLKSFYSAVKDAERVNLNVKWLHQRLDELVQAVNSMNLKDSKRKSMQRVESIKKALELKKVEMEEIQSKIQELEGQLASETLEEEILNITVTDITSKYEFLEHKCLTDGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AIP7</accession>
    <name>A0AA39AIP7_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_000346</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AIP7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015215">
        <property type="term" value="F:nucleotide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000075">
        <property type="entry name" value="Nicotinamide adenine dinucleotide transporter 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044712">
        <property type="entry name" value="SLC25A32-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45683">
        <property type="entry name" value="MITOCHONDRIAL NICOTINAMIDE ADENINE DINUCLEOTIDE TRANSPORTER 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="81"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="119"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="8">
        <location>
            <begin position="14"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="8">
        <location>
            <begin position="117"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="8">
        <location>
            <begin position="217"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="315"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="327"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="340"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9708241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="373" mass="40401" checksum="930D3DF53058C0FA" modified="2024-01-24" version="1">MTGPHQSSGAHSIRDTICYAASGAAAGAIAATFVCPLDVIKTRLQVHGLPEVRHSGVRGSVIITSLENIIRTEGLKGMYRGLSPTILALLPNWAVYFAVYEKLKGVLHSHVDSSSQLTIGANVIAASGAGAATAITTNPLWVVKTRLQTQAMRPNVVPYKGIFSALKRIAQEEGIRGLYSGLLPSLAGITHVAIQFPAYEQMKSYLAKMGDTKVDELGPGKVAIASSFSKVVASVMTYPHEVIRSRLQEQGQARNSEKHYSGVIDCIEKVYRKEGLPGFYRGCATNLLRTTPSAVITFTSFEMIHRFLQRVLHQDAKHSPANPNSDGHIKPQRENGRTVVEQNDSSVRQSQAQSTSNRTPIPIGNKEQLPASH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AIZ0</accession>
    <name>A0AA39AIZ0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein PLANT CADMIUM RESISTANCE 2-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_000503</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AIZ0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006461">
        <property type="entry name" value="PLAC_motif_containing"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01571">
        <property type="entry name" value="A_thal_Cys_rich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15907">
        <property type="entry name" value="DUF614 FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04749">
        <property type="entry name" value="PLAC8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="182"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="33"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9708495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="201" mass="22015" checksum="7923F63BB9E917C9" modified="2024-01-24" version="1">MYSSDDYRRFMTTPPPEPAPPSFNGAGTATGIPVSSPEPSFSEKPSQAPLQPKVKAPRVPWSSGLCDCFSDPRNCCITCWCPCITFGQIAEIVDKGSSACGVNGALYTLIACVTGCACCYSCFYRAKMRQQYLLKPSPCGDCLVHCCCEYCSLCQEYRELKNRGFDMTIGWHGNVERQNRGVEMSSMSSQTAPTVEEGMSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AJ71</accession>
    <name>A0AA39AJ71_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Aspergillus nuclease S1</fullName>
            <ecNumber evidence="4">3.1.30.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_000319</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage to 5'-phosphomononucleotide and 5'-phosphooligonucleotide end-products.</text>
            <dbReference type="EC" id="3.1.30.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nuclease type I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.30.1" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AJ71"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000014">
        <property type="term" value="F:single-stranded DNA endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006308">
        <property type="term" value="P:DNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11010">
        <property type="entry name" value="S1-P1_nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.575.10:FF:000002">
        <property type="entry name" value="Endonuclease 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.575.10">
        <property type="entry name" value="P1 Nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008947">
        <property type="entry name" value="PLipase_C/P1_nuclease_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003154">
        <property type="entry name" value="S1/P1nuclease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33146:SF27">
        <property type="entry name" value="ENDONUCLEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33146">
        <property type="entry name" value="ENDONUCLEASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02265">
        <property type="entry name" value="S1-P1_nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48537">
        <property type="entry name" value="Phospholipase C/P1 nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9708199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="274" mass="30543" checksum="C1F67E88EEFED54A" modified="2024-01-24" version="1">MAGVQTGISPFAGLLRFCSVSLPHLQSRLSDAAEDAVKELLPASANDDLASLCSWADRVKFRYHWSSPLHFIDTPDDLCTYQYTRDCKDEDGVKGRCVAGAINNYTSQLLTYGSSQADYNLTEALLFLSHLMGDVHQPLHVGFTTDKGGNTIDVKWYNTKTVLHHVWDTNIIETAEEQFYDSNIGDLIDAIEKNITTEWADQVPKWEICSGNKTACPDIYASEGIKAACNWSYKGVREDSVLEDDYFLSRLPIITFRLAQGGVRLAATLNRIFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39APP1</accession>
    <name>A0AA39APP1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_001225</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39APP1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11393">
        <property type="entry name" value="bHLH_AtbHLH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045239">
        <property type="entry name" value="bHLH95_bHLH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045843">
        <property type="entry name" value="IND-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16223:SF383">
        <property type="entry name" value="TRANSCRIPTION FACTOR BHLH111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16223">
        <property type="entry name" value="TRANSCRIPTION FACTOR BHLH83-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="7">
        <location>
            <begin position="389"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="346"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="347"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9709633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="508" mass="55797" checksum="868D42122D37D483" modified="2024-01-24" version="1">MAEECTDSSVATSSSAPNWWDLHGSSTLSSCYNSTNPWSQPNPNSNSSCEEEVSISTSFTNASNHSGLTVESSRRLIEPASSTNELIGEPAASDSHLWSHVLLNVGSNGDLHGSQDVGENLLDALSSKSLSTGIFEPACDYLKKMDNSWEFTNSTSFSNFDKHFNGFSENFIGSERLTKLSDLVSNWSIAPPDPEVNHQFDPQICNMSLSSPMDIQYSQPDLCHMKLTFNESASCGMGASRNSGLLSCYRHDQKVENDHHEIGSAPGPLLRRPCQSNGIGYQFGLNGSIVGDNGKYYYGIPNTTCSNPRNFADVISFSSRLCKPLVDSRESKPCLKPLSLSDCKKQGLQTSSQARNNARGQGISNEGKKKRSDQDTSEAQTVLKKPKQESSTVSSVKMQAPKVKLGERITALQQIVSPFGKTDTASVLYEAIGYIKFLQEQVQLLSNPYMKTNKDPWGGLDRKDKGDLKLDLKSRGLCLVPISCTPQVYRENTGSDYWPTTYRGCLYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D3L7</accession>
    <name>A0AA39D3L7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Metallophosphoesterase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_026465</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9669911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D3L7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056229">
        <property type="entry name" value="Ig_TMM62"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056230">
        <property type="entry name" value="TMEM62_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14795">
        <property type="entry name" value="HELICASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14795:SF0">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24384">
        <property type="entry name" value="Ig_TMM62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24394">
        <property type="entry name" value="TMEM62_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041275632" description="Metallophosphoesterase" evidence="2">
        <location>
            <begin position="25"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="493"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="537"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="606"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="671"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="703"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="3">
        <location>
            <begin position="50"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="TMEM62 Ig-like" evidence="4">
        <location>
            <begin position="345"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="domain" description="TMEM62 C-terminal" evidence="5">
        <location>
            <begin position="495"/>
            <end position="707"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF24384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF24394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9669911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="728" mass="82706" checksum="025D12DBCA1E7376" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D5W0</accession>
    <name>A0AA39D5W0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycine-rich protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_025746</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D5W0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010800">
        <property type="entry name" value="GRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37389:SF16">
        <property type="entry name" value="GLYCINE-RICH CELL WALL STRUCTURAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37389">
        <property type="entry name" value="NODULIN-24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07172">
        <property type="entry name" value="GRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041371808" description="Glycine-rich protein" evidence="2">
        <location>
            <begin position="27"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9672251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="108" mass="11289" checksum="13B19935F9EAF943" modified="2024-01-24" version="1">MAYSKTFLLVSLLFAVVLILSSEVSARELAETSQTQVSVEDAKYGGYGRGYGHEKGHGEEKGHGYGKGKGKGKGKPGHGKPGHPPVHGGAGEDVEEGTERTRLGVISP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D655</accession>
    <name>A0AA39D655_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_025677</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D655"/>
    <dbReference type="SMR" id="A0AA39D655"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01791">
        <property type="entry name" value="Ubl_UBL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000054">
        <property type="entry name" value="Ubiquitin-like protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039732">
        <property type="entry name" value="Hub1/Ubl5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13042">
        <property type="entry name" value="UBIQUITIN-LIKE PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="2">
        <location>
            <begin position="2"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9672129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="73" mass="8560" checksum="5480CE10291133F6" modified="2024-01-24" version="1">MIEVVLNDRLGKKVRVKCNDDDTIGDLKKLVAAQTGTRSDKIRIQKWYTIYKDHITLKDYEIHDGMGLELYYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D674</accession>
    <name>A0AA39D674_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">caffeoyl-CoA O-methyltransferase</fullName>
            <ecNumber evidence="3">2.1.1.104</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_026941</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Methylates caffeoyl-CoA to feruloyl-CoA and 5-hydroxyferuloyl-CoA to sinapoyl-CoA. Plays a role in the synthesis of feruloylated polysaccharides. Involved in the reinforcement of the plant cell wall. Also involved in the responding to wounding or pathogen challenge by the increased formation of cell wall-bound ferulic acid polymers.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(E)-caffeoyl-CoA + S-adenosyl-L-methionine = (E)-feruloyl-CoA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16925"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:87136"/>
            <dbReference type="ChEBI" id="CHEBI:87305"/>
            <dbReference type="EC" id="2.1.1.104"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Aromatic compound metabolism; phenylpropanoid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-dependent O-methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.104" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D674"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042409">
        <property type="term" value="F:caffeoyl-CoA O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009809">
        <property type="term" value="P:lignin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000147">
        <property type="entry name" value="Caffeoyl-CoA O-methyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050362">
        <property type="entry name" value="Cation-dep_OMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002935">
        <property type="entry name" value="SAM_O-MeTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10509:SF74">
        <property type="entry name" value="CAFFEOYL-COA O-METHYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10509">
        <property type="entry name" value="O-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01596">
        <property type="entry name" value="Methyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51682">
        <property type="entry name" value="SAM_OMT_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0438">Lignin biosynthesis</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9670717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="247" mass="27823" checksum="11935E99E482C1EE" modified="2024-01-24" version="1">MAGNQEGQQNQAGRHQEVGHKSLLQSDALYQYILETSVYPREPKPMKELRELTAKHPWNIMTTSADEGQFLNMLLKLINAKNTMEIGVYTGYSLLATALALPDDGKILAMDINRENYELGLPIIEKAGLAHKIDFREGPALPVLDQMVEDGKYHGTFDFIFVDADKDNYLNYHKRLIDLVRVGGVIGYDNTLWNGSVVAPPDAPLRKYVRYYKDFVLELNKALAADPRIEICQLPVGDGITLCRRIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D6V2</accession>
    <name>A0AA39D6V2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Retrovirus-related Pol polyprotein from transposon TNT 1-94</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_025205</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D6V2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025724">
        <property type="entry name" value="GAG-pre-integrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054722">
        <property type="entry name" value="PolX-like_BBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039537">
        <property type="entry name" value="Retrotran_Ty1/copia-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057670">
        <property type="entry name" value="SH3_retrovirus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648">
        <property type="entry name" value="TRANSPOSASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648:SF28">
        <property type="entry name" value="TRANSPOSON-ENCODED PROTEIN WITH RIBONUCLEASE H-LIKE AND RETROVIRUS ZINC FINGER-LIKE DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13976">
        <property type="entry name" value="gag_pre-integrs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22936">
        <property type="entry name" value="Pol_BBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25597">
        <property type="entry name" value="SH3_retrovirus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="7">
        <location>
            <begin position="34"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase catalytic" evidence="8">
        <location>
            <begin position="271"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9671392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1155" mass="131449" checksum="926A311494227BF3" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D820</accession>
    <name>A0AA39D820_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sulfotransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_023816</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D820"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052796">
        <property type="entry name" value="Nod_factor_sulfotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32175">
        <property type="entry name" value="PROTEIN, PUTATIVE, EXPRESSED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32175:SF21">
        <property type="entry name" value="SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="23"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9674486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="344" mass="39510" checksum="D3E84965F21FB9DF" modified="2024-01-24" version="1">MVDYVCFFNKDTLVIKTPKKSPLLLTMAVLVFAMVCGVYICAICLKQTSNNTKAKFLKFQVINQARPSFGIEPWEIPYVHYPKPKTHNREECVCNGVRYFAILSMQRSGSGWFETLLNSHINVSSNGEIFSIGNRRNNISSILSTMDKVYNLDWFSSASKNECLAAVGFKWMLNQGLMEHHKDIVKYFKNKGVSAIFLFRRNLLRRMISVLANSYDRDAKLLNGTHKSHVHSTEEAQILAKYKPTVNATLLLPDLKEAEEIITKALEYFKSTRHIVLYYEDLINNQTKLKDVQEFLRLPYRDLSSRQVKIHGGNLSKQVGNWDDVQKALKGTSYEGMLHADYQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D908</accession>
    <name>A0AA39D908_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">General transcription and DNA repair factor IIH subunit TFB4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">RNA polymerase II transcription factor B subunit 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_023288</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the 7-subunit TFIIH core complex composed of XPB, XPD, TFB1/GTF2H1, GTF2H2/P44, TFB4/GTF2H3, TFB2/GTF2H4 and TFB5/GTF2H5, which is active in NER. The core complex associates with the 3-subunit CDK-activating kinase (CAK) module composed of CYCH1/cyclin H1, CDKD and MAT1/At4g30820 to form the 10-subunit holoenzyme (holo-TFIIH) active in transcription.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the TFB4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D908"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000439">
        <property type="term" value="C:transcription factor TFIIH core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005675">
        <property type="term" value="C:transcription factor TFIIH holo complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000064">
        <property type="entry name" value="RNA polymerase II transcription factor B subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004600">
        <property type="entry name" value="TFIIH_Tfb4/GTF2H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12831:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIH SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12831">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIH TFIIH , POLYPEPTIDE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03850">
        <property type="entry name" value="Tfb4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9 11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9673642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="291" mass="31806" checksum="11AEE092A9E02F25" modified="2024-01-24" version="1">MAIGRSAVMAPVPSKLYSDDVSLLMVLLDTNPFFWSTASIPFSKFLSHVLAFLNSILLINQFNQVVVIATGCNSCNFIFDSSSVPANPNLENGRMPALCSNLLQKLEEFVTGDEKLSKEVLAAGIGSSLLSGSLSMALCYIQRVFRTGPLHPQPRILCLQGSPDGPEQYVAVMNAIFSAQRSMVPIDSCVLGAQHSAFLQQASYITGGVYLKPQQLDGLFQYLSTVFATDLHSRRFLQLPKPAGVDFRASCFCHKNTIDMGYICSVCLSIFCKHHKKCSTCGRKRQSSLEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D941</accession>
    <name>A0AA39D941_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_025115</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9676433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D941"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053288">
        <property type="entry name" value="TGD_Bridge_Protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34201">
        <property type="entry name" value="GLYCINE-RICH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34201:SF1">
        <property type="entry name" value="GLYCINE-RICH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9676433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="138" mass="14386" checksum="92AA028AF138B408" modified="2024-01-24" version="1">MLGGDDKEGDGEKGLLWKLPVLKSKQVGKLGPGFGFGAGCGIGLGVGLLGGIGFGPGIPGLQLGVGFGAGCGVGLGFGYGVGRGVAHDEYRRYSNVGKLFQGRDLPTQDDIGVLVDEVVRNTKKLIKATEREIEKWRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D9Z8</accession>
    <name>A0AA39D9Z8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">Pectate lyase</fullName>
            <ecNumber evidence="4 10">4.2.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_022370</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>Eliminative cleavage of (1-&gt;4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.</text>
            <dbReference type="EC" id="4.2.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="10">Binds 1 Ca(2+) ion. Required for its activity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 2/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the polysaccharide lyase 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.2.2" evidence="4 10"/>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D9Z8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030570">
        <property type="term" value="F:pectate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018082">
        <property type="entry name" value="AmbAllergen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002022">
        <property type="entry name" value="Pec_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007524">
        <property type="entry name" value="Pec_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045032">
        <property type="entry name" value="PEL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31683">
        <property type="entry name" value="PECTATE LYASE 18-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31683:SF69">
        <property type="entry name" value="PECTATE LYASE 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04431">
        <property type="entry name" value="Pec_lyase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00544">
        <property type="entry name" value="Pectate_lyase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00807">
        <property type="entry name" value="AMBALLERGEN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00656">
        <property type="entry name" value="Amb_all"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 10" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041482469" description="Pectate lyase" evidence="10">
        <location>
            <begin position="24"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="domain" description="Pectate lyase" evidence="11">
        <location>
            <begin position="158"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9677348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="431" mass="48120" checksum="0305B4912D6A1B26" modified="2024-01-24" version="1">MEAVNMRLLVLIPFLVIVPAVTANIGDFDEVWQTRAEEARKAALQAYNPHPEKVTDNFNKKVHKSTNHTRRNLSKFSGPCMATNPIDRCWRCRSDWASNRMKLADCVLGFGQQTTGGKGGKIYVVTDLSDNDMVNPKPGTLRHAVIQEEPLWIIFAHSMAIRLNEELIMTSNKTIDARGANVHIANGAGLTLQFVHNIIIHGLHIHDIKVGNGGLIRDSASHYGFRTRSDGDGISIFGATNIWIDHISMSNCADGLIDAIMASTAITISNCHFTHHNEVMLFGASDSFSGDAIMQITVTFNHFGQGLTQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTILSQGNRFIAPPDINCKEVTKRDYSPESVWKSWTWRSQGDLMMNGAFFVKSGDPNFDFSNKYVIRAKPGTFVTRLTRFSGALSCREGMPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DAR2</accession>
    <name>A0AA39DAR2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Morc S5 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PVL29_021872</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MORC ATPase protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9676570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DAR2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031349">
        <property type="term" value="P:positive regulation of defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000075">
        <property type="entry name" value="MORC family CW-type zinc finger protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045261">
        <property type="entry name" value="MORC_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041006">
        <property type="entry name" value="Morc_S5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23336:SF58">
        <property type="entry name" value="PROTEIN MICRORCHIDIA 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23336">
        <property type="entry name" value="ZINC FINGER CW-TYPE COILED-COIL DOMAIN PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13589">
        <property type="entry name" value="HATPase_c_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17942">
        <property type="entry name" value="Morc6_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="14" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="13" id="KW-0943">RNA-mediated gene silencing</keyword>
    <feature type="domain" description="Morc S5" evidence="17">
        <location>
            <begin position="403"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="18"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="568"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="44"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="622"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="641"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="686"/>
            <end position="703"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF17942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ9676570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="830" mass="93335" checksum="E48D732A07C8DEEA" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DAX9</accession>
    <name>A0AA39DAX9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Chlorophyll a-b binding protein, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PVL29_022434</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">The light-harvesting complex (LHC) functions as a light receptor, it captures and delivers excitation energy to photosystems with which it is closely associated.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast thylakoid membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the light-harvesting chlorophyll a/b-binding (LHC) protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DAX9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009522">
        <property type="term" value="C:photosystem I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009523">
        <property type="term" value="C:photosystem II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016168">
        <property type="term" value="F:chlorophyll binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009768">
        <property type="term" value="P:photosynthesis, light harvesting in photosystem I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3460.10:FF:000002">
        <property type="entry name" value="Chlorophyll a-b binding protein, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3460.10">
        <property type="entry name" value="Chlorophyll a/b binding protein domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001344">
        <property type="entry name" value="Chloro_AB-bd_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022796">
        <property type="entry name" value="Chloroa_b-bind"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21649">
        <property type="entry name" value="CHLOROPHYLL A/B BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00504">
        <property type="entry name" value="Chloroa_b-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103511">
        <property type="entry name" value="Chlorophyll a-b binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 16 17" id="KW-0148">Chlorophyll</keyword>
    <keyword evidence="4 17" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="13 17" id="KW-0157">Chromophore</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 17" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="9 17" id="KW-0603">Photosystem I</keyword>
    <keyword evidence="17" id="KW-0604">Photosystem II</keyword>
    <keyword evidence="6 17" id="KW-0934">Plastid</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14 17" id="KW-0793">Thylakoid</keyword>
    <keyword evidence="11" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="binding site" evidence="16">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="16">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>chlorophyll b</name>
            <dbReference type="ChEBI" id="CHEBI:61721"/>
            <label>1</label>
        </ligand>
        <ligandPart>
            <name>Mg</name>
            <dbReference type="ChEBI" id="CHEBI:25107"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="16">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>chlorophyll b</name>
            <dbReference type="ChEBI" id="CHEBI:61721"/>
            <label>1</label>
        </ligand>
        <ligandPart>
            <name>Mg</name>
            <dbReference type="ChEBI" id="CHEBI:25107"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR601344-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU363080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ9677441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="252" mass="27925" checksum="ACF82100FBC31C01" modified="2024-01-24" version="1">MATVTTQASAAVFRPCASKSRFLTGSSGKLGREVSVKPMTSSSYRSFKVEAKKGEWLPGLSSPSYLDGSLPGDNGFDPLGLAEDPENLKWYIQAELVNSRWAMLGVAGMLLPEVFTKIGIINVPQWYDAGKAEYFASSSTLFVIEFILFHYVEIRRWQDIKNPGSVNQDPIFKNYSLPPNEVGYPGGIFNPLNFAPTEEAKEKELANGRLAMLAFLGFIVQHNVTGKGPFDNLLQHLSDPWHNTIIQTLRGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DDB4</accession>
    <name>A0AA39DDB4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ZF-HD dimerization-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_022833</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9678067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DDB4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000257">
        <property type="entry name" value="Zinc-finger homeodomain protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006455">
        <property type="entry name" value="Homeodomain_ZF_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006456">
        <property type="entry name" value="ZF_HD_homeobox_Cys/His_dimer"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01565">
        <property type="entry name" value="homeo_ZF_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01566">
        <property type="entry name" value="ZF_HD_prot_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31948:SF16">
        <property type="entry name" value="ZINC-FINGER HOMEODOMAIN PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31948">
        <property type="entry name" value="ZINC-FINGER HOMEODOMAIN PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04770">
        <property type="entry name" value="ZF-HD_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51523">
        <property type="entry name" value="ZF_HD_DIMER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0371">Homeobox</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZF-HD dimerization-type" evidence="11">
        <location>
            <begin position="6"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="60"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9678067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="155" mass="17319" checksum="42F6FAD66B680CE5" modified="2024-01-24" version="1">MEGELYRECLRNHAASLGSYATDGCGEFTLDATSPGGLLCAACGCHRNFHRKLISSTPFAEGRQQQSAAAVESPESERSGGHKKRLRTKFTADQKEKMLAFAEKIRWKMQRKEEEDEIERFCRGVGVSRQVFKVWMHNHKNSSSSSSSASTTLTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DDX9</accession>
    <name>A0AA39DDX9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_020781</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DDX9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34189">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34189:SF4">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="56"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="127"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9678697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="148" mass="16570" checksum="143F5A9D906B27F8" modified="2024-01-24" version="1">MYRSLSSTRVAEDYNINVGSPSSKSPGLRVSVSNEELPVYDPVSEMAKKERSRIKFAENAVHVIPFVLLLCAIILWVFSNPDVDVGIKNDSIAARIKGLSLEGDEIDEITTNNLLPTLEFGELEHSRKQAKDHKAIRRTKAKSKFKSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DDY1</accession>
    <name>A0AA39DDY1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">PGG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_019749</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DDY1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026961">
        <property type="entry name" value="PGG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24186:SF56">
        <property type="entry name" value="PGG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24186">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13962">
        <property type="entry name" value="PGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="31"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="133"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="PGG" evidence="8">
        <location>
            <begin position="27"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9680516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="167" mass="18662" checksum="1037F28B7E5D3271" modified="2024-01-24" version="1">MAERPKNNRKSWFRYFQYDEGRESPGDARNVLLIVAGLIAAVTFQAGVNPPGGVWQDDENGHHAGRAIYSYQTREFYIFLISNTVALSTSILILISLTCRFPFQFEIILATASMVVTYASAIFAVTPDDLVKFRYILAAAAAPFAIRCLLQMWNGMKVKVTDGEVDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DE43</accession>
    <name>A0AA39DE43_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_019690</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DE43"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056562">
        <property type="entry name" value="LysM2_CERK1_LYK3_4_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056563">
        <property type="entry name" value="LysM3_LYK4_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036779">
        <property type="entry name" value="LysM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056561">
        <property type="entry name" value="NFP_LYK_LysM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052611">
        <property type="entry name" value="Plant_RLK_LysM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45927">
        <property type="entry name" value="LYSM-DOMAIN RECEPTOR-LIKE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45927:SF6">
        <property type="entry name" value="PROTEIN LYK5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23446">
        <property type="entry name" value="LysM1_NFP_LYK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23472">
        <property type="entry name" value="LysM2_CERK1_LYK3_4_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23473">
        <property type="entry name" value="LysM3_LYK4_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54106">
        <property type="entry name" value="LysM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="NFP/LYK4/5 first LysM" evidence="2">
        <location>
            <begin position="66"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="LYK3/4/5 second LysM" evidence="3">
        <location>
            <begin position="126"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="LYK4/5 third LysM" evidence="4">
        <location>
            <begin position="197"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9680439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="272" mass="30559" checksum="77D28DD66C590482" modified="2024-01-24" version="1">MHMEMEKSFNSPLYFVFGWWWCRLVLLFWVWISAAQAQQSYISGGQFNCENHFKETDGFLCNGPRSCLSYLTFRSAPPYDSPRSISSLLNSDPAQIASINKVSVDYKIPLNTPLIVPVNCSCSGDFYQYNASYTANASDTYFSIANNIYQGLTTCQAVKAQNPSHNSSGYLSAEMDLLIPLRCACPVDVLISSGYNYLLSYIIDEGENISFIAQTFHVEIWTIYAANELPYSQDYLGVSTALLVPLKDPPTAFQTRRTSNCIPNDALVYEIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DF01</accession>
    <name>A0AA39DF01_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_017819</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DEAD box helicase family. DDX21/DDX50 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9681596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DF01"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00268">
        <property type="entry name" value="DEADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12938">
        <property type="entry name" value="GUCT_Hera"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001060">
        <property type="entry name" value="ATP-dependent RNA helicase RhlE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000911">
        <property type="entry name" value="Nucleolar RNA helicase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059027">
        <property type="entry name" value="DD_DDX21-DDX50"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044742">
        <property type="entry name" value="DEAD/DEAH_RhlB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012562">
        <property type="entry name" value="GUCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF1">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26142">
        <property type="entry name" value="DD_DDX21-DDX50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08152">
        <property type="entry name" value="GUCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="16">
        <location>
            <begin position="116"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
        <location>
            <begin position="147"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="15">
        <location>
            <begin position="354"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="13">
        <location>
            <begin position="745"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="630"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="11">
        <location>
            <begin position="116"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="659"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="702"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="723"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="754"/>
            <end position="764"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9681596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="764" mass="83450" checksum="29BAA1D755E6CD97" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DGN3</accession>
    <name>A0AA39DGN3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">acyl-CoA hydrolase</fullName>
            <ecNumber evidence="8">3.1.2.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_020382</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a fatty acyl-CoA + H2O = a fatty acid + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16781"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:77636"/>
            <dbReference type="EC" id="3.1.2.20"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; fatty acid metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the C/M/P thioester hydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.20" evidence="8"/>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9681487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DGN3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047617">
        <property type="term" value="F:fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006637">
        <property type="term" value="P:acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009062">
        <property type="term" value="P:fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03444">
        <property type="entry name" value="Thioesterase_II_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03445">
        <property type="entry name" value="Thioesterase_II_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.210:FF:000003">
        <property type="entry name" value="Acyl-CoA thioesterase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000109">
        <property type="entry name" value="Acyl-CoA thioesterase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.210">
        <property type="entry name" value="Acyl-CoA thioesterase, double hotdog domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042171">
        <property type="entry name" value="Acyl-CoA_hotdog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003703">
        <property type="entry name" value="Acyl_CoA_thio"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049449">
        <property type="entry name" value="TesB_ACOT8-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025652">
        <property type="entry name" value="TesB_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00189">
        <property type="entry name" value="tesB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11066">
        <property type="entry name" value="ACYL-COA THIOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11066:SF34">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13622">
        <property type="entry name" value="4HBT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02551">
        <property type="entry name" value="Acyl_CoA_thio"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="9">
        <location>
            <begin position="15"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9681487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="450" mass="51345" checksum="1A2E0822462D03FA" modified="2024-01-24" version="1">MDTESVIEFLGCVPLLQRLPSSSLRKIAEVVRTKHYDRGEFVVHEGEMGDGIYFIWEGEAEVAGSVTAEQESRLEFQLKRYDYFGHGIGASVQQANVIALSKLICLVLPHEHCTLLQPISIWNADKALEACSLVELILHLEPIEVNIFKGITLPDAPKFGKVFGGQLLGQALAAASKTVDCLKVVHSLHAYFLLVGDFNMPIIYEVRRLRDGKSFATRRVDAIQKGNVIFTLLASFQKEEEGFDHQVATMPSVPAPDTLLSLEELRDRRLTDPRLPRTYRNKVAAKEFIPWPIEIRFCEPNNSTNQTKSPPSLRYWFRAKGKLSDDQALHRCVAAYASDLIFLSVSLNPHRKKDLKTTSVSLDHSMWFHRPFRADDWLLFVIASPAAYNARGFCAGQMFNRKGEVNNSYPYVWFFKNFKRKIKNRFKINKFSHVPSSLFIFSLSYFFFHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DGX8</accession>
    <name>A0AA39DGX8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S ribosomal protein L7a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_018984</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DGX8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000003">
        <property type="entry name" value="60S ribosomal protein L7a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050257">
        <property type="entry name" value="eL8/uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004037">
        <property type="entry name" value="Ribosomal_eL8-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018492">
        <property type="entry name" value="Ribosomal_eL8/Nhp2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001921">
        <property type="entry name" value="Ribosomal_eL8_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23105">
        <property type="entry name" value="RIBOSOMAL PROTEIN L7AE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00881">
        <property type="entry name" value="L7ARS6FAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00882">
        <property type="entry name" value="RIBOSOMALL7A"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01082">
        <property type="entry name" value="RIBOSOMAL_L7AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="5">
        <location>
            <begin position="116"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9683215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="259" mass="29412" checksum="611A1345D49EC5F7" modified="2024-01-24" version="1">MAPKKGVKAPAALAKKKQEKVVNPLFEKRPKQFGIGGALPPKRDLSRFVKFPKHVQIQRKRRILRQRLKVPPALNQFTKTLDKNLATSLFKMLLKYRPEDKAAKKERLLKRAQAEAEGKTAETKKPIVVKYGLNHVTYLIEQNKAQLVVIAHDVDPIELVVWLPALCRKMEIPYCIVKGKSRLGSIVHKKTASVLCLTTVKNEDKLEFSKILEAIKANFNDKFDENRKKWGGGIMGSKSQARTKAKERLLAKEAAQRMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DIH2</accession>
    <name>A0AA39DIH2_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_017026</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DIH2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004252">
        <property type="entry name" value="Probable_transposase_24"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33144:SF50">
        <property type="entry name" value="OS03G0714750 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33144">
        <property type="entry name" value="OS10G0409366 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03004">
        <property type="entry name" value="Transposase_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="284"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9684835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="358" mass="41320" checksum="3ACC07F4D1D733BA" modified="2024-01-24" version="1">MSHRRGRVQIVSPEDELDNLQLLDIQPAATTTLSSSDPSDASDPSVVGMIKKIVFNFTYDKCSSSSKKRTRGPTRNLDLLSMKPEEKKSTRFNTRGQVVYDGKGERLSIQDWNHVSEDVKEKIWALVLEKYELEKTCKSYILQCCGNLFRIYRNKMKAKYYNPYNTDEERLCHRPPHLSDDDWRWLIHFWGTLEAKHTTGSKSYAQIRYEQQLLSQPEGTSSSTSASSGSSTSVASTYVDEIYTQVMGPERHGHVRGYGFGLTPTSIFGSTSRRRLGAILSTQLENAQEMLIVAEQKFTTATEELSHVKETFEERLIEVQRKTLEEVKIEFEEKMMEMQRKMQAQIQEQMMQMMQQFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DIZ3</accession>
    <name>A0AA39DIZ3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transducin/WD40 repeat-like superfamily protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_017678</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DIZ3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000127">
        <property type="term" value="C:transcription factor TFIIIC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006383">
        <property type="term" value="P:transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000777">
        <property type="entry name" value="Transducin/WD40 repeat-like superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017956">
        <property type="entry name" value="AT_hook_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052416">
        <property type="entry name" value="GTF3C_component"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000637">
        <property type="entry name" value="HMGI/Y_DNA-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15052:SF2">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR 3C POLYPEPTIDE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15052">
        <property type="entry name" value="RNA POLYMERASE III TRANSCRIPTION INITIATION FACTOR COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00929">
        <property type="entry name" value="ATHOOK"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00354">
        <property type="entry name" value="HMGI_Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="204"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="361"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="785"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="217"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="228"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="361"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="376"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="391"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="793"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="810"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="840"/>
            <end position="857"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9685734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="935" mass="103595" checksum="66F67F4EC985C207" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DKM8</accession>
    <name>A0AA39DKM8_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_017549</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DKM8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9685552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="131" mass="14800" checksum="407327CCC09F5289" modified="2024-01-24" version="1">MTTIYHSFSSNSTHSEKLDSISPKLHVAVTKHGFESNLPVMNSILDMYCRCSLFSKLCLLAIFTARNLLTGLLENIGRLSRLIHLDFHQNSDYAGISSIPASIKGCCSLVEFYMGITCCLCYQQRLRHFLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DMP6</accession>
    <name>A0AA39DMP6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_014688</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C48 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9689155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DMP6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016929">
        <property type="term" value="F:deSUMOylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016926">
        <property type="term" value="P:protein desumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.395.10">
        <property type="entry name" value="Adenoviral Proteinase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12606:SF157">
        <property type="entry name" value="OS06G0122600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12606">
        <property type="entry name" value="SENTRIN/SUMO-SPECIFIC PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="5">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9689155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="180" mass="21077" checksum="A17EAD62F0866B8D" modified="2024-01-24" version="1">MIFCMSLQPFFDRKTSILSKYISKLHDCKKLFIPMHDNCPGHWYLCIIDFKNSHIQILDSLRSRSRDEFRFKSVKIVVEFCQTFFKLYDIGKDVFQFSVDWAPSILTQDNGWDCGVHVIKHMQTFQNGDCIKASNLRNSIKIHREIASDLVLHKGNREKQTILVIVCTNTSTRVMRSLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DRI6</accession>
    <name>A0AA39DRI6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histidine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Histidyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_010060</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>tRNA(His) + L-histidine + ATP = L-histidyl-tRNA(His) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17313"/>
            <dbReference type="Rhea" id="RHEA-COMP:9665"/>
            <dbReference type="Rhea" id="RHEA-COMP:9689"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78527"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.21"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.21" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DRI6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004821">
        <property type="term" value="F:histidine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006427">
        <property type="term" value="P:histidyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00773">
        <property type="entry name" value="HisRS-like_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000054">
        <property type="entry name" value="Histidine--tRNA ligase chloroplastic/mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.800:FF:000017">
        <property type="entry name" value="Histidine--tRNA ligase chloroplastic/mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00127">
        <property type="entry name" value="His_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015807">
        <property type="entry name" value="His-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041715">
        <property type="entry name" value="HisRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004516">
        <property type="entry name" value="HisRS/HisZ"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00442">
        <property type="entry name" value="hisS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43707:SF1">
        <property type="entry name" value="HISTIDINE--TRNA LIGASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43707">
        <property type="entry name" value="HISTIDYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13393">
        <property type="entry name" value="tRNA-synt_His"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001549">
        <property type="entry name" value="His-tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041267736" description="histidine--tRNA ligase" evidence="12">
        <location>
            <begin position="16"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="13">
        <location>
            <begin position="92"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="53"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="53"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="163"/>
            <end position="165"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="351"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="355"/>
            <end position="356"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001549-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9694388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="505" mass="56371" checksum="0B4035B575B6302F" modified="2024-01-24" version="1">MPVHASLLILKPSLATTFLRSSLSSALVPFPLIRLRPFPNPRNLSFLSSAASSPTHSLVSSSGDNVGGGRSGAISPPPIEDSVQKIDVNPPRGTRDFPPEDMRLRNWLFNHFREVSRSFGFEEVDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKSVSLPLKWFAVGQCWRYERMTRGRRREHYQWNMDIIGVPEVMAEAELISSIVTFFKRVGITASDVGFKVSSRKVLQEVLRCYSIPENLFGKVCVIIDKMEKIPIDEITKELKSLGMSEEAIKDLLQVLSIKSLTKLEEILGAAGEAVADLKQLFSLAEKFGYSEWIQFDASVVRGLAYYTGIVFEGFDREGKLRAICGGGRYDHLLSTFGGDDVPACGFGFGDAVIIELLKEKGLLPELSLQVENIVCALDHYLQGAAAAVATRLREKGQSVDLVLESKPLKWVFKRAARTNAQRLILVGNSEWQRGMVSVKILSSGEQYEIKLDELE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DUD2</accession>
    <name>A0AA39DUD2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_009198</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DUD2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039537">
        <property type="entry name" value="Retrotran_Ty1/copia-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057670">
        <property type="entry name" value="SH3_retrovirus"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648">
        <property type="entry name" value="TRANSPOSASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648:SF28">
        <property type="entry name" value="TRANSPOSON-ENCODED PROTEIN WITH RIBONUCLEASE H-LIKE AND RETROVIRUS ZINC FINGER-LIKE DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25597">
        <property type="entry name" value="SH3_retrovirus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="3">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9697298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="536" mass="60327" checksum="D6890A67D5A1DE3B" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E1I2</accession>
    <name>A0AA39E1I2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Mitochondrial adenine nucleotide transporter ADNT1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_001613</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E1I2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000116">
        <property type="entry name" value="Mitochondrial substrate carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24089">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="38"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="141"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="244"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9706136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="354" mass="38277" checksum="E6552EA17FDED4AD" modified="2024-01-24" version="1">MASEDVKTSEAAVSTIVNLAEEAKLAREGVKAPSHALLSVCKSLVAGGVAGGVSRTAVAPLERLKILLQVQNPHTIKYNGTIQGLKYIWKSEGFRGLFKGNGTNCARIVPNSAVKFYSYEQASKGILWLYRQQPGNEDAELTPLLRLGAGACAGIIAMSATYPMDMVRGRLTVQTEKSPRQYRGIYHALSTVLREEGARAVYKGWLPSVIGVIPYVGLNFAVYESLKDWLMKAKPFGIVEDSELGVTTRLACGAAAGTVGQTVAYPLDVIRRRMQMVGWKDAASVVTGDGRGKAPLEYTGMVDAFRKTVRHEGFGALYKGLVPNSVKVVPSIAIAFVTYEMVKDILGVEMRISD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E1U5</accession>
    <name>A0AA39E1U5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_005411</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9704109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E1U5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33710">
        <property type="entry name" value="BNAC02G09200D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33710:SF64">
        <property type="entry name" value="ENDONUCLEASE_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="1">
        <location>
            <begin position="6"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9704109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="357" mass="42058" checksum="2DB0B3B26B229EEC" modified="2024-01-24" version="1">MNLKILSWNVRGVNDSSKRKLIKSIVRKQKVDLLCIQETKMQVMSEGVVRSLGSGIFLDWRVLNAIRSAGGILICWDKRSMEILEWEEGQFSISCKFKNVDDGVVWVFTGVYGPFSREERECLWEEIRAIRGLWEEPWCLGGDFNTILYHSERNDLRLVDFPLQGGSYTWSGGLNNQSWARLDRFLATPSWCDQYSRVSQRRLPRPTSDHFPVLLEGGGLRRGPSPFRFENMWLKVEGFKDLIRCWWQGMVVRGSPSYRLSVKLKELKQNLKVWNREVFGNLERNKAETLQQVEFWDSVEGERGLTEEELIRKKEAKEGYAKWVLLEETHWRQLSREIWLREGDRNMGYFHRMTNVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E285</accession>
    <name>A0AA39E285_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Chitin-binding type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_002902</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 19 family. Chitinase class I subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E285"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004568">
        <property type="term" value="F:chitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016998">
        <property type="term" value="P:cell wall macromolecule catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050832">
        <property type="term" value="P:defense response to fungus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00325">
        <property type="entry name" value="chitinase_GH19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06921">
        <property type="entry name" value="ChtBD1_GH19_hevein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.10:FF:000001">
        <property type="entry name" value="Basic endochitinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.20.10:FF:000001">
        <property type="entry name" value="Endochitinase (Chitinase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.530.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.20.10">
        <property type="entry name" value="Endochitinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.10">
        <property type="entry name" value="Endochitinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001002">
        <property type="entry name" value="Chitin-bd_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018371">
        <property type="entry name" value="Chitin-binding_1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036861">
        <property type="entry name" value="Endochitinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016283">
        <property type="entry name" value="Glyco_hydro_19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000726">
        <property type="entry name" value="Glyco_hydro_19_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22595:SF79">
        <property type="entry name" value="CHITINASE 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22595">
        <property type="entry name" value="CHITINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00187">
        <property type="entry name" value="Chitin_bind_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00182">
        <property type="entry name" value="Glyco_hydro_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001060">
        <property type="entry name" value="Endochitinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00451">
        <property type="entry name" value="CHITINBINDNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00270">
        <property type="entry name" value="ChtBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="Lysozyme-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57016">
        <property type="entry name" value="Plant lectins/antimicrobial peptides"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00026">
        <property type="entry name" value="CHIT_BIND_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50941">
        <property type="entry name" value="CHIT_BIND_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00773">
        <property type="entry name" value="CHITINASE_19_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00774">
        <property type="entry name" value="CHITINASE_19_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-0147">Chitin-binding</keyword>
    <keyword evidence="5 7 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0611">Plant defense</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041205129" description="Chitin-binding type-1 domain-containing protein" evidence="9">
        <location>
            <begin position="21"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-1" evidence="10">
        <location>
            <begin position="20"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="130"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7 8">
        <location>
            <begin position="23"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7 8">
        <location>
            <begin position="32"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7 8">
        <location>
            <begin position="37"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7 8">
        <location>
            <begin position="55"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="86"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="160"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001060-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001060-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9704559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="304" mass="32533" checksum="30B8C17733AE651F" modified="2024-01-24" version="1">MGLWALVAFCLLSLVLVGSAEQCGRQAGGRVCPGGACCSKFGWCGNTADYCGNGCQSQCSSTGDIGQLITRSMFNDMLKHRNEGSCPGKGFYTYDAFIAAAKAFPGFGTTGDTTTRKREIAAFLAQTSHETTGGWPSAPDGPYAWGYCYLREQGSPGAYCVPSAQWPCAAGRKYYGRGPIQISYNYNYGQAGKAIGVDLINNPDLVATDAVISFKTAFWFWMTPQSPKPSCHNVITGGWTPSAADRSAGRLPGYGVITNIINGGVQDRIGFYKRYCNILGVSYGDNLDCNNQRPFGSGLLLDTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E2C9</accession>
    <name>A0AA39E2C9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Sugar phosphate transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_004342</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E2C9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004853">
        <property type="entry name" value="Sugar_P_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050186">
        <property type="entry name" value="TPT_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03151">
        <property type="entry name" value="TPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="48"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="85"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="109"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="202"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="263"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="290"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="Sugar phosphate transporter" evidence="7">
        <location>
            <begin position="21"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="316"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9702562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="350" mass="38631" checksum="B77C64BD74774E42" modified="2024-01-24" version="1">MAKDGGSISDGLLRKILLSYAYVGLWIFLSFTVIVYNKYILDPKMYNWPFPISLTLIHMAFCSSIAFFLIRILKAVEPVSMSRQLYISSVVPIGALYSLSLWLSNSAYIYLSVSFIQMLKALMPVAVYTIGVLFKKEAFKSDTMCNMISISLGVAVAAYGEARFDSWGVCLQLGAVAFEATRLVLIQILLASKGISLNPITSLYYVAPCCLVFLLVPWIFVELPLLKNNSSFQFDFVIFGTNSLCAFALNLAVFLLVGKTSALTMNVAGVVKDWLLIAFSWSVIKDTVTPINLFGYGLAFLGVGYYNHSKLQALKSKEAQKKTTQTDEEAGRLLEDREGEAAGKKSDSEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E2E6</accession>
    <name>A0AA39E2E6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Disease resistance RPP13-like protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_003722</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E2E6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF1076">
        <property type="entry name" value="LEUCINE-RICH REPEAT (LRR) FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0611">Plant defense</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="4">
        <location>
            <begin position="172"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="5">
        <location>
            <begin position="364"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="260"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="653"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="662"/>
            <end position="674"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9705758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="674" mass="76261" checksum="24A2350F92D59DB8" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E2V4</accession>
    <name>A0AA39E2V4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycosyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_003275</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="5"/>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E2V4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080043">
        <property type="term" value="F:quercetin 3-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080044">
        <property type="term" value="F:quercetin 7-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000019">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000101">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058980">
        <property type="entry name" value="Glyco_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926">
        <property type="entry name" value="GLUCOSYL/GLUCURONOSYL TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926:SF986">
        <property type="entry name" value="UDP-GLYCOSYLTRANSFERASE 84A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26168">
        <property type="entry name" value="Glyco_transf_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase N-terminal" evidence="6">
        <location>
            <begin position="8"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF26168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9705144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="478" mass="53781" checksum="F62D0FE99AD7D09F" modified="2024-01-24" version="1">MGSESKLVHVFLVSFPGQGHVNPLIRLGKRLASKGLLVTFSTPESIGKQMRKASNITDQPTPVGEGLIRFEFFEDEWDENEPKRQDLDLYLPQLELVGKKVLPQMIKKHAEQDRPVSCLINNPFIPWVSDVAADLGIPSAMLWVQSCACFSTYYHYYHGLVPFPSEAEPEIDVQLPCMPLLKYDEVASFLYPTTPYPFLRRAILGQYRNLDKPFCILMDTLEELEPEVIEYMSKICPIKPVGPLYKNPKVPNAAVRGDFMKADNCIEWLDSKPPSSVVYVSFGSVVYLKQDQVDEIAYGLLNSGVQFLWVMKPPHKDAGLELLVLPEGFLEKVGDKGKVVQWSPQEQVLAHPSVACFVTHCGWNSSMEALSSSMPVVAFPQWGDQVTDAKYLVDEFKIGVRMCRGEAENKLITRDEVEKCLIKATTGPKAAEMKQNAMKWKKAAEQAVAEGGSSERNLQGFVDDVRRRSIEIIYKTKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E3V2</accession>
    <name>A0AA39E3V2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_005397</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9704092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E3V2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009941">
        <property type="term" value="C:chloroplast envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03225">
        <property type="entry name" value="ABC_cobalt_CbiO_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001360">
        <property type="entry name" value="ABC transporter I family member 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015856">
        <property type="entry name" value="ABC_transpr_CbiO/EcfA_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050334">
        <property type="entry name" value="Molybdenum_import_ModC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43514">
        <property type="entry name" value="ABC TRANSPORTER I FAMILY MEMBER 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43514:SF4">
        <property type="entry name" value="ABC TRANSPORTER I FAMILY MEMBER 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="41"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9704092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="271" mass="29557" checksum="1697A8420647387F" modified="2024-01-24" version="1">MNYSALSRSSALHLPNLHSTNPTRSVSLSIRSCIVTQNNAIEGQNLNYSVTTRLGQLTPILKDCSIRIPSGQLWMLLGPNGCGKSTLLKILAGLLNPTSGTLYVKKPKSFVFQNPDHQVVMPTVEADVAFGLGKFDLTLEEVRSRVSRALDAVGMSDYMQRPVQTLSGGQKQRVAIAGALAEACKVLLLDELTTFLDENDQVGVIKAVKNSVDSSGEVTALWVTHRMEELEYADGAVYMEDGRVVMHGNAASIMDFVKARQSSYLNQLKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E5U6</accession>
    <name>A0AA39E5U6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">PVL29_000200</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="25">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="21">Serine/threonine-protein kinase involved in the spatial and temporal control system organizing cortical activities in mitotic and postmitotic cells. Required for the normal functioning of the plasma membrane in developing pollen. Involved in the regulation of cell expansion and embryo development.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="25">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06627">
        <property type="entry name" value="STKc_Cdc7_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000372">
        <property type="entry name" value="MAP3K epsilon protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000278">
        <property type="entry name" value="MAP3K epsilon protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000304">
        <property type="entry name" value="MAP3K epsilon protein kinase 1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052441">
        <property type="entry name" value="Armadillo-Ser/Thr_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46618">
        <property type="entry name" value="ARMADILLO REPEAT-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46618:SF1">
        <property type="entry name" value="ARMADILLO REPEAT-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="14 22" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="18" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="9" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 22" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="24">
        <location>
            <begin position="20"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="296"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="385"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="846"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="925"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="846"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00054419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="KAJ9708024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1426" mass="157554" checksum="A0C5A370C277B296" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E9W4</accession>
    <name>A0AA39E9W4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein DETOXIFICATION</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Multidrug and toxic compound extrusion protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_000392</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E9W4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13132">
        <property type="entry name" value="MATE_eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045069">
        <property type="entry name" value="MATE_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="47"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="84"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="129"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="162"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="191"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="226"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="266"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="305"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="344"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="420"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="446"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9708322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="511" mass="55482" checksum="E19CB80E080B2B19" modified="2024-01-24" version="1">MAMEGGETTCLQSPLIGHQGDSHEFDGGTSGQGCCRREIIEEAKKQLWLAGPLVAVSMLQYCLQVISIMFVGHLGELPLSGASMATSFASVTGFSLLLGIGSALETLCGQAYGAREYHMVGVHTQRAMLTLLVLSIPLAFIWFFTAPILISLGQDRSISTEAGIFNRWMIPSLFAYGLLQCLNRFLQTQNIVFPIMISSGITALSHILVCWLLVFKSGLGSKGAALANTISNWVNVFLLAVYVKFSPVCSKTWTGLSKQALHDIPNFLKLAVPSATMICFEYWTFEMIVLLSGLLPNPKLEASVLSISLNTCWMVYTISVGLGGAISTRVSNELGAKHPQHARLAVWVVIIISISEGVVVGIATILIRHVWGKLYSNEAEVIKYVAKMTPLLALSNFLDGFQCVLSGAARGYGWQNICTIINLGAYYVVGIPCSVLFAFICNFGGMGLWMGIICGLCIQVTALVTMNLCTNWDEGVGASLNAWVFHPSLHDKPMTRDEENEEAIVLDYGHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YGL2</accession>
    <name>A0AA38YGL2_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_026556</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YGL2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041344321" evidence="2">
        <location>
            <begin position="30"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="100"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="145"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9670069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="227" mass="25351" checksum="EAEED0580E1B2324" modified="2024-01-24" version="1">MAPLRYFSFRQVLVLYLILFLFASTLVCACSESQPRRKQFLGRRRVFEIEKEEQPKKKTPSTSLSTKIQTKLVKEVQKEDQPKKKLDGTSSSKNQTKLVKPSTYSTKTTPTILNKTQLKKLNSTAKSSNSTKMTPTISAKKQLKKLNSTSKASNSTKSSSGTTKKSSDLLKPSTPNDKKSKPVIIKQTKTQTSEKFVGGPPPTAQDQLEGSRDLRHLFSHDLPVFQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YJG1</accession>
    <name>A0AA38YJG1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_025314</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YJG1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035251">
        <property type="term" value="F:UDP-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000037">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000087">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050481">
        <property type="entry name" value="UDP-glycosyltransf_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48049">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48049:SF91">
        <property type="entry name" value="UDP-GLYCOSYLTRANSFERASE 79B7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9671552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="463" mass="50711" checksum="E6C57A31683CD050" modified="2024-01-24" version="1">MAMAMPKSPKFHVAMFPWFAFGHMTAFLHLSNKLAQRGHKITFMLPTKAQSQLQTLNFHPTLISFHPLSIPHVDGLPPGAETASDIPIFLTHLLATAMDRATDQLEAALRASNPDFLFYDCSHLAPVLASRLGIKAICYNVVCAASIAIALVPARGVSKDRPVTEAELALPPSGYPSSTAVFRRHEGRALQFISAPFGDGISFYERITTAMKESDAISIRTCREIEGNLCDYIGTQYGKPVFLTGPVLPEPSPTPLEDRWAQWLGGFKPGSIIFCAFGSQYILEKDQFQELVLGLELTGLPFLVALKPPTGATTIEEALPEGFKERVGGRGAVHGGWVQQPSILSHPSVGCFVSHCGFGSMWESLMNDCQIVLIPHLGDQILNTRVLAGELQVAVEVEREENGWFSKESLCKAIKSVMDEESEEGGSVRKNHAKWKETFASPGFMSNYVDKFVGQLQGLLDHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YJQ0</accession>
    <name>A0AA38YJQ0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_025414</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YJQ0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041221027" description="Secreted protein" evidence="2">
        <location>
            <begin position="23"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9671687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="81" mass="8219" checksum="0EA08CE54103F8AF" modified="2024-01-24" version="1">MTWISVSFSLWLILITPALVGGSRGHSSSKEAALVGGSRVYSSSKVAAVTARGAVPPSTSSSCTHIGLGKGDHRPCPPSTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YM11</accession>
    <name>A0AA38YM11_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_026264</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YM11"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9672933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="115" mass="12853" checksum="A2F1E6178B3ED3FD" modified="2024-01-24" version="1">MSVIGNNRSGVVGENGFPPFTNCYLPIPTPSLAASSCTKSSIAMTYKSAGLRFPPTLVTILSAYLSSLYFMGGLVMRFRYGVFRLELRIYYKKDLSKLWVAMHGLKWPCMRVTDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YMA1</accession>
    <name>A0AA38YMA1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">DEAD-box ATP-dependent RNA helicase 36</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_026387</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the DEAD box helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9673081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YMA1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17955">
        <property type="entry name" value="DEADc_DDX49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF24">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 6" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="10">
        <location>
            <begin position="51"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="8">
        <location>
            <begin position="82"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="9">
        <location>
            <begin position="282"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="469"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="5">
        <location>
            <begin position="51"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="41"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="472"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9673081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="489" mass="54758" checksum="27B14080BB10C4DE" modified="2024-01-24" version="1">MDEETLVDENFPLFSKRKPTPKPSKNSTTTAQNPNPEQRLTRTTNPTSPSSTFADLGLAEWAAQTCKELGMRRPTPVQHHCIPRILAGDDVLGLAQTGSGKTAAFALPILHRLAEDPFGVFALVVTPTRELAYQLAEQFRALGSCLHLRCAVVVGGMDMINQAQTLMQRPHVVIATPGRVKVLLEQNPDIPAVFARTKFLVLDEADRVLDVGFEEELRVVFQCLPKNRQTLLFSATMTSDLQTLLELSANKAYFYEAYEGFKTVESLKQQYIFVPKNVKDVYLLHILSKMEEMGIRSAIIFVSTCRSCHLLSLLLEELDLEVAALHSFKSQSLRLAAVHRFKSGQVPILLATDVASRGLDIPTVDLVVNYDIPRYPRDYVHRVGRTARAGRGGLSLSIVTQNDVDLIHEIEAVLGKQLDKFDCKEKEVLDNITKVYKARRVSTMKMMDDGFEEKAKARKAQKLKTLAEKGLLKKKTRKRKREKASKIGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YMR3</accession>
    <name>A0AA38YMR3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_023091</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YMR3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16664">
        <property type="entry name" value="RING-Ubox_PUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045210">
        <property type="entry name" value="RING-Ubox_PUB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052608">
        <property type="entry name" value="U-box_domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45958">
        <property type="entry name" value="RING-TYPE E3 UBIQUITIN TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45958:SF6">
        <property type="entry name" value="U-BOX DOMAIN-CONTAINING PROTEIN 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04564">
        <property type="entry name" value="U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51698">
        <property type="entry name" value="U_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="U-box" evidence="7">
        <location>
            <begin position="28"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9673287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="813" mass="89801" checksum="F1336E0B8FCF0699" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YPE8</accession>
    <name>A0AA38YPE8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Inositol-tetrakisphosphate 1-kinase</fullName>
            <ecNumber evidence="9">2.7.1.134</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_023589</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Kinase that can phosphorylate various inositol polyphosphate such as Ins(3,4,5,6)P4 or Ins(1,3,4)P3.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>1D-myo-inositol 3,4,5,6-tetrakisphosphate + ATP = 1D-myo-inositol 1,3,4,5,6-pentakisphosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12452"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57539"/>
            <dbReference type="ChEBI" id="CHEBI:57733"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.134"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9 11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="9 11">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 9">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the ITPK1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.134" evidence="9"/>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YPE8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052726">
        <property type="term" value="F:inositol-1,3,4-trisphosphate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052725">
        <property type="term" value="F:inositol-1,3,4-trisphosphate 6-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047325">
        <property type="term" value="F:inositol-3,4,5,6-tetrakisphosphate 1-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032957">
        <property type="term" value="P:inositol trisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008656">
        <property type="entry name" value="Inositol_tetrakis-P_1-kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040464">
        <property type="entry name" value="InsP(3)kin_ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041429">
        <property type="entry name" value="ITPK1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14217">
        <property type="entry name" value="INOSITOL-TETRAKISPHOSPHATE 1-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14217:SF39">
        <property type="entry name" value="INOSITOL-TETRAKISPHOSPHATE 1-KINASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05770">
        <property type="entry name" value="Ins134_P3_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17927">
        <property type="entry name" value="Ins134_P3_kin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038186">
        <property type="entry name" value="ITPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 9 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 9 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ATP-grasp" evidence="12">
        <location>
            <begin position="133"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>1D-myo-inositol 1,3,4-trisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58414"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>1D-myo-inositol 1,3,4-trisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58414"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>1D-myo-inositol 1,3,4-trisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58414"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="204"/>
            <end position="215"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>1D-myo-inositol 1,3,4-trisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58414"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="310"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="310"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="312"/>
        </location>
        <ligand>
            <name>1D-myo-inositol 1,3,4-trisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58414"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="312"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="316"/>
        </location>
        <ligand>
            <name>1D-myo-inositol 1,3,4-trisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58414"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR038186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR038186-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR038186-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9674127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="347" mass="39230" checksum="D16516CCCDDF4B5C" modified="2024-01-24" version="1">MRLNMNEEGREEEEEMIQGRFSIGEVGGFQKPMKLVVVGYALTSKKTKSFLQPKLEGLARNKGILFVAIDQNRSLSEQGPFDIVLHKLSGKEWRQILEDYRQTHPEVTVLDPPDAIQHVHNRQFMLQDVADLNLSNSYGKVGVPKQLVVKRDASSIPDAVTKAGLKLPLVAKPLVVDGSAKSHELSLAYDQYSLQKLEPPLVLQEFVNHGGVLFKVYIVGEAIKVVRRFSLPDVTKRELSKNAGVFRFPRVSCAAASADDADLDPCVAELPPWPLLERLARELRRRLGLRLFNLDIIREHGTRDRFYVIDINYFPGYGKMPEYEHIFTDFLLSLAESNYKRLSGSNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YQK0</accession>
    <name>A0AA38YQK0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Proteasome subunit alpha type</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_023964</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the 20S core complex of the 26S proteasome. The 26S proteasome is composed of a core protease (CP), known as the 20S proteasome, capped at one or both ends by the 19S regulatory particle (RP/PA700). The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the peptidase T1A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YQK0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03749">
        <property type="entry name" value="proteasome_alpha_type_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000016">
        <property type="entry name" value="Proteasome subunit alpha type-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035144">
        <property type="entry name" value="Proteasome_alpha1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3 5 6" id="KW-0647">Proteasome</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="8">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="242"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9674747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="284" mass="30738" checksum="955C976C780603E7" modified="2024-01-24" version="1">MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSKTHVVLACVNKANSELSSHQKKIFKVDDHIGVAIAGLTADGRVLSRYMRSECINYSFTYESPLPIGRLVVQLADKAQVCTQRSWKRPYGVGLLVAGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFETFQNSSREDLIKDALFAIRETLQGEKLKSSICTVTVLGVGEPFHILDQETVQGLINAFEMAGEEAPAADQGAAGEEGAAGEEGAAAEEGAATEEGAATEEGAAPMEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YRG1</accession>
    <name>A0AA38YRG1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_024304</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the plant DMP1 protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YRG1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010256">
        <property type="term" value="P:endomembrane system organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007770">
        <property type="entry name" value="DMP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31621:SF37">
        <property type="entry name" value="OS01G0882400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31621">
        <property type="entry name" value="PROTEIN DMP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05078">
        <property type="entry name" value="DUF679"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="78"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="135"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="176"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9675286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="213" mass="24095" checksum="0BA4BAAE65CA919B" modified="2024-01-24" version="1">MDNSLQQDLIESYLPDNGSYYCLGDDGTDESHFIYVLNAILSGTARLNVLLPTATILAFTIFAPLLTNDGKCHTLNRWLMGFFLAISAASCIFFLFTDSFRTARGRLYYGVATRNGIWTFNGGRRKPCTPSEYRLRWIDLFHALLSLIAFLTFAGLHNDVLECYHLEIPRKVTNSVPLVVGFVISVLFVVFPSKRRGIGYPFLLQKDALYTRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YUM2</accession>
    <name>A0AA38YUM2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Core-2/I-branching beta-1,6-N-acetylglucosaminyltransferase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_022130</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9676983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YUM2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044174">
        <property type="entry name" value="BC10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003406">
        <property type="entry name" value="Glyco_trans_14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31042">
        <property type="entry name" value="CORE-2/I-BRANCHING BETA-1,6-N-ACETYLGLUCOSAMINYLTRANSFERASE FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31042:SF20">
        <property type="entry name" value="CORE-2_I-BRANCHING BETA-1,6-N-ACETYLGLUCOSAMINYLTRANSFERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02485">
        <property type="entry name" value="Branch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="68"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="74"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9676983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="415" mass="47185" checksum="F429EFD3FC59673D" modified="2024-01-24" version="1">MGNEHQRLPMVSLFFNSNLVLRHVFHLLYFVIGLSLGIMVGFYVKNSSFDLQAIISFTAPPPAAAPPTLLVTLPPTPSPSPSPLPQPPSASASASPSPSPSPYPLPDGTLASQDKPTLMHSMDDDELFWRASMVPRIQQFPYEHVPKVAFMFLTKGPIPLGPLWDLFFKGHEGFYSIYVHPHPSYNDSVPEDSVFHGRRIPSKAVKWGRATMIDAERRLLANALLDFSNERFVLLSETCIPLFNFTTIYSYIINSNQSFIASYDDPRKVGRGRYNPQMSPTINISEWRKGSQWFEVHRELAIEIVSDKKYYPVFREHCHIPCYLDEHYIPTLINILAPEKNSNRTITWVDWSKSGPHPGRFIRQDVTLEFLNQTRFGNNCTYGGNTTSMCFLFARKFVPNTLQPLLQLLSLDFSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YVE7</accession>
    <name>A0AA38YVE7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_022404</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YVE7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47447">
        <property type="entry name" value="OS03G0856100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47447:SF22">
        <property type="entry name" value="TETRATRICOPEPTIDE-LIKE HELICAL DOMAIN SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12854">
        <property type="entry name" value="PPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="17"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="75"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="110"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="145"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="180"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="215"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="284"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="319"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="354"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="389"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="424"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="459"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="494"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="529"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="564"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="600"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="635"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="670"/>
            <end position="704"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9677406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="764" mass="86226" checksum="9989B5A6BCDBF10F" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YW93</accession>
    <name>A0AA38YW93_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PB1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_022695</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YW93"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06410">
        <property type="entry name" value="PB1_UP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053198">
        <property type="entry name" value="Gynoecium_Dev_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000270">
        <property type="entry name" value="PB1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31066">
        <property type="entry name" value="OS05G0427100 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31066:SF66">
        <property type="entry name" value="PB1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00564">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00666">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PB1" evidence="2">
        <location>
            <begin position="27"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="119"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="160"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9677868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="204" mass="22431" checksum="D4AD9E310AE15D86" modified="2024-01-24" version="1">MGGDLDSANNRIIKFLYSYGGKILPRRIDGKLRYVGGHTRVLAVDRSISYAELMVKLGELCGSSVTLRCQLPKEDLDVLVTVTSDEELANVIDEYHRASSSCSKIRAVLSPPKSLKTISPVSSTTSSVDSSDSRSITSVRAHRSASPPMLAYRYPGPRSVSPTTTANRCSTRISPPIEFPLYVRWDTAKLYRNPCHVGGSPKYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YWP3</accession>
    <name>A0AA38YWP3_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_022854</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9678091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YWP3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.90">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9678091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="181" mass="20240" checksum="90B453FAA46ACF66" modified="2024-01-24" version="1">MFTQGSGDLIASAVQSKSAVDQMFNNSSLSSSPKSVLPEKVPVDQVSTWNVKENNGETTDELLEPLLLCCESLQSCGSGILGMHSLEDCLQAWEILPKVNYAMSGYELEMLHIRGFQLSREQEKCIGKYFLGKFTPYSLFSPFSLCILRAREVEFIFNTLISLFVCELGVRLSISHFYCLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YY64</accession>
    <name>A0AA38YY64_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_020933</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9678885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YY64"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:002340">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318:SF124">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318">
        <property type="entry name" value="PARTNER OF PAIRED, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9678885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="485" mass="54420" checksum="846CC04EA8864A46" modified="2024-01-24" version="1">MSYSYSPEKQNPYLPLKKSPSSWSNLWFKSPNKALNHVVFTMHLKSLTKRQLQPHKALIFDRTLLLSDELLLRVFQKLPESQRKPSSLVCKRWLSLQGRLVRSLKLLEWDFLESGRLVSRFPNLTRVDLVHACIVWPRSCGVLLNHRVVSVPVDSEVSQNGFLENGKVLASNVVDRGLRFLASGYPNLRKLAVVGASELGLLSLAEECSTLQELELHKCSDATLRAISGFGNLQILKLIGNAEGLYKSLVSDVGLTILAQGCARLVKLELSGCEGSYDGIKAIGQCCQMLEELTLSDHRLDGGWLSALYYCENLKTLRFQSCRRIDVYPGLDEYLGSCPTLERLHLHKCQLRDKLSMRALYMVCGAVRDFVVQDCWGLDNDILGLATTCRRVKLLSLEGCSLLTTEGLESAVLSWKDLQKLRVVSCKNIKDSEVSPAVSTLFSILKELQWRPDTRSLLSSSLVESGMGKRGSKFFKKTWRPEVPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YZ24</accession>
    <name>A0AA38YZ24_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Stilbene synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_021297</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>4-coumaroyl-CoA + 3 malonyl-CoA + 3 H(+) = trans-resveratrol + 4 CO2 + 4 CoA</text>
            <dbReference type="Rhea" id="RHEA:11936"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:45713"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57355"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="EC" id="2.3.1.95"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the thiolase-like superfamily. Chalcone/stilbene synthases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YZ24"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050350">
        <property type="term" value="F:trihydroxystilbene synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030639">
        <property type="term" value="P:polyketide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00831">
        <property type="entry name" value="CHS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000014">
        <property type="entry name" value="Chalcone synthase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000025">
        <property type="entry name" value="Chalcone synthase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012328">
        <property type="entry name" value="Chalcone/stilbene_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001099">
        <property type="entry name" value="Chalcone/stilbene_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018088">
        <property type="entry name" value="Chalcone/stilbene_synthase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011141">
        <property type="entry name" value="Polyketide_synthase_type-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11877:SF14">
        <property type="entry name" value="CHALCONE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11877">
        <property type="entry name" value="HYDROXYMETHYLGLUTARYL-COA SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02797">
        <property type="entry name" value="Chal_sti_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00195">
        <property type="entry name" value="Chal_sti_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000451">
        <property type="entry name" value="PKS_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00441">
        <property type="entry name" value="CHALCONE_SYNTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Chalcone/stilbene synthase N-terminal" evidence="7">
        <location>
            <begin position="5"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Chalcone/stilbene synthase C-terminal" evidence="8">
        <location>
            <begin position="238"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="5">
        <location>
            <position position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000451-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9679326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="392" mass="42904" checksum="50243A85F3E3E0EA" modified="2024-01-24" version="1">MASVEEFRNAQRAKGPATILAIGTATPDHCVYQSDYADYYFRVTKSEHMTELKKKFNRICDKSMIKKRYIHLTEEMLEEHPNIGAYMAPSLNIRQEIITAEVPKLGKEAALKALKEWGQPKSKITHLVFCTTSGVEMPGADYKLANLLGLETSVRRVMLYHQGCYAGGTVLRTAKDLAENNAGARVLVVCSEITVVTFRGPSEEALDSLVGQALFGDGSAAVIVGSDPDISIERPLFQLVSAAQTFIPNSAGAIAGNLREVGLTFHLWPNVPTLISENIEKCLTQAFDPLGISDWNSLFWIAHPGGPAILDAFEAKLNLEKKKLEATRHVLSEYGNMSSACVLFIMDEMRKRSLKGEKATTGDGLDWGVLFGFGPGLTIETVVLHSIPTVTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YZC2</accession>
    <name>A0AA38YZC2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_021255</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant LTP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YZC2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01960">
        <property type="entry name" value="nsLTP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.110.10">
        <property type="entry name" value="Plant lipid-transfer and hydrophobic proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036312">
        <property type="entry name" value="Bifun_inhib/LTP/seed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016140">
        <property type="entry name" value="Bifunc_inhib/LTP/seed_store"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000528">
        <property type="entry name" value="Plant_nsLTP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33076">
        <property type="entry name" value="NON-SPECIFIC LIPID-TRANSFER PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00234">
        <property type="entry name" value="Tryp_alpha_amyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00382">
        <property type="entry name" value="LIPIDTRNSFER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47699">
        <property type="entry name" value="Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00597">
        <property type="entry name" value="PLANT_LTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041260890" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein" evidence="2">
        <location>
            <begin position="34"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical" evidence="3">
        <location>
            <begin position="37"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9679272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="133" mass="13692" checksum="23715240B47D2F68" modified="2024-01-24" version="1">MVRMVISHGMGGQKAVKVMGILMVLSWAQSTMAAIDCSTVTALVSACSTFITYGAPDPIPGSPCCDAVVGLNSIADSIDNRRSVCRCLMGLIATYNPNATAIATLPGFCGISLGFNLDPNTDCNLVDGAASNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z0B9</accession>
    <name>A0AA38Z0B9_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_021792</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9680028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z0B9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33868">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33868:SF2">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="316"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="141"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="24"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9680028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="346" mass="39512" checksum="737364EF20FBB610" modified="2024-01-24" version="1">MAAAESRAGWKRNTNRCFLHENASRAPNLSSFPSSSSSSKRQSDGRPGDAAHWSDHPSPDCMHQNCNPLEDPAPDSKWWLYPQPNFGHQKGFEYEQLNTLENEFDILSYEFINQTAIEGLGAQTETKKNADFFPDRSRKVSATRMKEDQFARMSEPKTGPHGNPQDIGKDKDIEELWYTDEDLDPVNSLVSEQSKKLSSDLESHWMGAKNTEPWWRKADKDTLASMVAQKSVEHIENCDLPKPQIKHFRRGLSASLEWSDQDRMVTPSLDQMAELGFSNLTDCTWKSHTSASIDEKQSSLGAVEYSPNRSDTMFRYFLLQTFLLGILGFVPCLKLCFQEYYKYIGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z1E8</accession>
    <name>A0AA38Z1E8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_019731</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025724">
        <property type="entry name" value="GAG-pre-integrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054722">
        <property type="entry name" value="PolX-like_BBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039537">
        <property type="entry name" value="Retrotran_Ty1/copia-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057670">
        <property type="entry name" value="SH3_retrovirus"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648">
        <property type="entry name" value="TRANSPOSASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648:SF28">
        <property type="entry name" value="TRANSPOSON-ENCODED PROTEIN WITH RIBONUCLEASE H-LIKE AND RETROVIRUS ZINC FINGER-LIKE DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13976">
        <property type="entry name" value="gag_pre-integrs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22936">
        <property type="entry name" value="Pol_BBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25597">
        <property type="entry name" value="SH3_retrovirus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="6">
        <location>
            <begin position="464"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="178"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="205"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9680495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1332" mass="151345" checksum="2BEB185B8035EF60" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z205</accession>
    <name>A0AA38Z205_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NAD-dependent epimerase/dehydratase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_019955</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z205"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08958">
        <property type="entry name" value="FR_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000984">
        <property type="entry name" value="Dihydroflavonol 4-reductase family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF563">
        <property type="entry name" value="CINNAMOYL-COA REDUCTASE 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="3">
        <location>
            <begin position="8"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9680795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="335" mass="36975" checksum="79FBCB1C4DC6E01D" modified="2024-01-24" version="1">MEGEKGRVCVTGGTGFIASWLVMKLLQQGYSVNATIRSHPQSKKDISYLTNLPGASERLQIFKADLSDPNSFDAAIEGCIGVFHVAHPVDFEEREPQETVTKRSVAGTLGILKGCLKSKTVKRVVYTSSAAAVVYNNKDEDIMDESSWSDIDVVKSLKPLGWSYAISKTITERAALEFAEQHGLDLVTLIPSLVVGPFICPGFPGSVHMTLALIFGNQNHYQYLKNTSMVHVDDVASAFIFLLEYPNAKGRHICSSDIITLNEMSELLSAKYPQLPIPTIESLKDIQGFRMPGVSSKKLLDTGFKYKYGVDEMFDEAIQCCKEKGFLYFSKDTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z423</accession>
    <name>A0AA38Z423_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PVL29_020419</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">ATP-dependent RNA helicase involved in mRNA turnover, and more specifically in mRNA decapping.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">P-body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DEAD box helicase family. DDX6/DHH1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9681539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z423"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd17940">
        <property type="entry name" value="DEADc_DDX6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000114">
        <property type="entry name" value="ATP-dependent RNA helicase DDX6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000364">
        <property type="entry name" value="ATP-dependent RNA helicase DDX6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47960">
        <property type="entry name" value="DEAD-BOX ATP-DEPENDENT RNA HELICASE 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 13" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="17">
        <location>
            <begin position="117"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="15">
        <location>
            <begin position="148"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="16">
        <location>
            <begin position="328"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="82"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="12">
        <location>
            <begin position="117"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="11"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="82"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ9681539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="491" mass="55933" checksum="2734B4C77C792810" modified="2024-01-24" version="1">MHARGRYPPGIHGGGRGGGMGSGNPSGPNPNFQYRNPNQQQYVQRNVGQSHQQFQQQNQQQWLRRNQAGPDSAVDEVEKTVQSEAVDSSSQDWKARLKIPPPDTRYRTEDVTATKGNEFEDYFLKRELLMGIYEKGFERPSPIQEESIPIALTGSDILARAKNGTGKTAAFCIPALEKIDQDNNVIQVVILVPTRELALQTSQVCKELGKHLKIQVMVTTGGTSLKDDIMRLYQPVHLLVGTPGRILDLSKKGVCILKDCSMLVMDEADKLLSPEFQPSIEQLIHFMPLNRQILLFSATFPVTVKDFKDRYLQKPYIINLMDELTLKGITQYYAFVEERQKVHCLNTLFSKLQINQSIIFCNSVNRVELLAKKITELGYSCFYIHAKMLQDHRNRVFHDFRNGACRNLVCTDLFTRGIDIQAVNVVINFDFPKNSETYLHRVGRSGRFGHLGLAVNLITYEDRFNLYRIEQELGTEIKQIPPHIDQAIYCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z4X6</accession>
    <name>A0AA38Z4X6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_018388</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057135">
        <property type="entry name" value="At4g27190-like_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050905">
        <property type="entry name" value="Plant_NBS-LRR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463:SF198">
        <property type="entry name" value="RPP4C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23247">
        <property type="entry name" value="LRR_RPS2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NB-ARC" evidence="6">
        <location>
            <begin position="158"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein At4g27190-like leucine-rich repeats" evidence="7">
        <location>
            <begin position="800"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein At4g27190-like leucine-rich repeats" evidence="7">
        <location>
            <begin position="927"/>
            <end position="1018"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein At4g27190-like leucine-rich repeats" evidence="7">
        <location>
            <begin position="1084"/>
            <end position="1213"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein At4g27190-like leucine-rich repeats" evidence="7">
        <location>
            <begin position="1362"/>
            <end position="1490"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein At4g27190-like leucine-rich repeats" evidence="7">
        <location>
            <begin position="1522"/>
            <end position="1654"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein At4g27190-like leucine-rich repeats" evidence="7">
        <location>
            <begin position="1655"/>
            <end position="1769"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="27"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9682458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1810" mass="204153" checksum="B162209BE1E13F52" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z632</accession>
    <name>A0AA38Z632_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_018792</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z632"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000449">
        <property type="entry name" value="MYB-related transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052844">
        <property type="entry name" value="Leaf_Dev_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47214">
        <property type="entry name" value="PROTEIN ROUGH SHEATH 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47214:SF1">
        <property type="entry name" value="PROTEIN ROUGH SHEATH 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="10">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="9">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="9">
        <location>
            <begin position="54"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="10">
        <location>
            <begin position="58"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="112"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="211"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="281"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="124"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="216"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9682940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="384" mass="43502" checksum="F51829954852F58E" modified="2024-01-24" version="1">MKERQRWQPEEDALLRAYVKQYGAKEWNLISHRMGKSLERDPKSCLERWKNYLKPGIKKGSLTLEEQNLVISLQAKYGNKWKKIASEVPGRTAKRLGKWWEVFKEKQLKQLHKTTHLRHDHSDSPSSNAVATASAPSPDHGKYDHILETFAEKYVQPKLLAFHPLPLPIMPNLSLSDPPPVLSLGSVGISDAGVPVGSTTPSPVLPPWMNATNMGSTTSSISSSSSTPSPSVSLSLSPSEPAVLDPVHPEASRLMPVQQMGTLIQYCKELEEGRQNWVQHKKEATWRLSRLEQQLESEKSRKRREKMEEIEGKIRCLREEEMAHLGRIESDYREQLIAVQRDAESKEAKLMETWGSKHVKLAKLVEKIGIHTPTQAFTLPKDLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z6A5</accession>
    <name>A0AA38Z6A5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF4219 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_018879</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z6A5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35317">
        <property type="entry name" value="OS04G0629600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35317:SF28">
        <property type="entry name" value="ZINC FINGER, CCHC-TYPE, RIBONUCLEASE H-LIKE DOMAIN, GAG-PRE-INTEGRASE DOMAIN PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9683074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="114" mass="13527" checksum="18D7B5F9D117514F" modified="2024-01-24" version="1">MVVNNNSIHSQLPKFTGKNFDNWCIQMRVLFRSQDLWDLINIDYNKVIDSKEFKALSREQKGSLKDFRKKDKKTLYAIFQVVDESIFEKILEAETTKESWVTLQKLYKGDKCVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z7R7</accession>
    <name>A0AA38Z7R7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Plasma membrane ATPase</fullName>
            <ecNumber evidence="16">7.1.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PVL29_019242</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15 16">
            <text>ATP + H2O + H(+)(in) = ADP + phosphate + 2 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:20852"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Cell membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIIA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.1" evidence="16"/>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z7R7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008553">
        <property type="term" value="F:P-type proton-exporting transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120029">
        <property type="term" value="P:proton export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02076">
        <property type="entry name" value="P-type_ATPase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000045">
        <property type="entry name" value="ATPase 4 plasma membrane-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000004">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000004">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000211">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006534">
        <property type="entry name" value="P-type_ATPase_IIIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01647">
        <property type="entry name" value="ATPase-IIIA_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42861">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 16" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="13 16" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 16" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 16" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 16" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="65"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="100"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="246"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="281"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="649"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="714"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="792"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="825"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="17">
        <location>
            <begin position="17"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU362083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ9683592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="955" mass="104835" checksum="57523F100E222939" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z811</accession>
    <name>A0AA38Z811_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_016561</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z811"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007750">
        <property type="entry name" value="DUF674"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33103">
        <property type="entry name" value="OS01G0153900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33103:SF19">
        <property type="entry name" value="OS09G0544700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05056">
        <property type="entry name" value="DUF674"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9684126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="147" mass="16554" checksum="9CD4364190AE6D48" modified="2024-01-24" version="1">MPKKVYMCGSYHRFIADDPRAICPQCKYTLSTEVPYVGPPPATSESYQRDGGYVKGIVTYMVNDDLVVKHMSTISGIALLRNFNVKEVGVLEEKVVEVTLSEGLKLLKASLQTKEVLTSVFLSKKIIYLIAPHFKIFILVFVIRDHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z949</accession>
    <name>A0AA38Z949_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Carboxypeptidase</fullName>
            <ecNumber evidence="10">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_016846</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the peptidase S10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="10"/>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z949"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11320:FF:000002">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12670:FF:000002">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000013">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033124">
        <property type="entry name" value="Ser_caboxypep_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF306">
        <property type="entry name" value="SERINE CARBOXYPEPTIDASE-LIKE 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00560">
        <property type="entry name" value="CARBOXYPEPT_SER_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 10" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041483139" description="Carboxypeptidase" evidence="10">
        <location>
            <begin position="32"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9684574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="470" mass="52798" checksum="4B8226D4EEF1A641" modified="2024-01-24" version="1">MGGRECHPSFSSLFCCVLGFFILLVSSGATAGNREDQVRDRIVKLPGEPPNVGFSQYSGYITVDPRAGRALFYWLIEAPARRGPSRPLILWLNGGPGCSSVAYGASEEVGPFRVRPDGKTLHLNRYAWNAEANLLFLDSPAGVGFSYSNTSSDIPHVGDKRTANDAYKFLINWLQRFPQYKHRPFYIAGESYAGHYIPELSQIIVQRNKGMKNPAINFKGFLLGNPLIDDFYDYKGTHEFWWNHGLISDSTYEALKEACANDSFLFPKDKCNKTLTGAYKEIGDIDLYNIYSGPCRQVATLGSNSKLPLPLNFRGNDECIVRYTKKYMNRGEVQKAFHANVTHLPYSWAACSSIVRRNWSDSPKSMLPIFKQLISAGIRIWLYSGDTDAVLPLTATRYSINALKLKTIRNWYAWYDKKEVGGWSQVYNGLTFATVRGAGHEVPLSQPRRALILLEHFLNNKPMPAAPTLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z9I8</accession>
    <name>A0AA38Z9I8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-synthesizing GTPase</fullName>
            <ecNumber evidence="3">3.6.5.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_016975</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.3"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.3" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z9I8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005850">
        <property type="term" value="C:eukaryotic translation initiation factor 2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01888">
        <property type="entry name" value="eIF2_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03688">
        <property type="entry name" value="eIF2_gamma_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15490">
        <property type="entry name" value="eIF2_gamma_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000009">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000011">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000065">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050543">
        <property type="entry name" value="eIF2G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015256">
        <property type="entry name" value="eIF2g_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044127">
        <property type="entry name" value="eIF2g_dom_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044128">
        <property type="entry name" value="eIF2g_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003077">
        <property type="entry name" value="PRK04000.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42854">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42854:SF3">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09173">
        <property type="entry name" value="eIF2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="10">
        <location>
            <begin position="32"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="Initiation factor eIF2 gamma C-terminal" evidence="11">
        <location>
            <begin position="347"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF09173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9684767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="449" mass="49330" checksum="43B92B33EC714DD1" modified="2024-01-24" version="1">MSRRGLMEQDLSKLDVTTLHPLSPEVISRQATINIGTIGHVAHGKSTVVKAISGVQTVRFKNELERNITIKLGYANAKLYKCEDERCPRPMCYKAYGSGKEDGPLCDVPGFENCRMKLLRHVSFVDCPGHDILMATMLNGAAIMDGALLLIAANESCPQPQTAEHLAAVEIMRLQHIIILQNKVDLIQENVAINQHEAIQKFIQGTVADGAPVVPISAQLKYNIDVVCEYIVKKIPIPERDFTSPPNMIVIRSFDVNKPGFEGVLKVNQFIEVRPGIVVKDENGNIKCTPIYSRIVSLYAEQNELQFAVPGGLIGVGTTMDPTLTRADRLVGQVLGEVGSLPDVFVELEVNFFLLRRLIGVRTKGTEKQGKVSKLSKGEILMLNIGSMSTGARVLAVRNDLAKLQLTSPVCTSRGEKIALSRRVEKHWRLIGWGQIQAGTTLDIPANPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZCB6</accession>
    <name>A0AA38ZCB6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_015017</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Probable RNA-binding protein that plays a role in meiosis and vegetative growth.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9685932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZCB6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045927">
        <property type="term" value="P:positive regulation of growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045836">
        <property type="term" value="P:positive regulation of meiotic nuclear division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12524">
        <property type="entry name" value="RRM1_MEI2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12529">
        <property type="entry name" value="RRM2_MEI2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12531">
        <property type="entry name" value="RRM3_MEI2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000063">
        <property type="entry name" value="MEI2-like protein 5 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000101">
        <property type="entry name" value="Protein MEI2-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034453">
        <property type="entry name" value="MEI2-like_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034454">
        <property type="entry name" value="MEI2-like_RRM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007201">
        <property type="entry name" value="Mei2-like_Rrm_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23189">
        <property type="entry name" value="RNA RECOGNITION MOTIF-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04059">
        <property type="entry name" value="RRM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0469">Meiosis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="188"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="273"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="66"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="467"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="83"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="479"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="511"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="536"/>
            <end position="548"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00058438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9685932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="843" mass="92094" checksum="C6C6FED5AF49C0B5" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZCP8</accession>
    <name>A0AA38ZCP8_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_015042</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the plant Proton pump-interactor protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9685964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZCP8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055282">
        <property type="entry name" value="PPI1-4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32219:SF16">
        <property type="entry name" value="CORE-2_I-BRANCHING BETA-1,6-N-ACETYLGLUCOSAMINYLTRANSFERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32219">
        <property type="entry name" value="RNA-BINDING PROTEIN YLMH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="27"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="178"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9685964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="288" mass="33549" checksum="3E368FDF1BF33857" modified="2024-01-24" version="1">MVAQVNFMDSSLDELPASEQIPQSYEELNVKARIEEAEKMIQKWNQDGQQIAEKLRSKMLDWNSTTFRLEAVKCPDQWLKIRQVLKRKDLEPLQLALDRFCFANKACRGRTNIGSCSSAEGLNPFSFHYKMLHKAKNLGEEKQLLRKIDGSQRRDVDSCISVENLNYRIKMLYNKMEWERNSSEKKQLLRNIKELEVERDKALSNSNSHLLGSKEDIQNHIKIISNDLDQIRKEHLPIKAKIKHVEKEMNVIEKDVGSLQKKLAAINRKKDKAYNSVLEWKKLLGEAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZH35</accession>
    <name>A0AA38ZH35_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">glycerophosphodiester phosphodiesterase</fullName>
            <ecNumber evidence="1">3.1.4.46</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_014546</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a sn-glycero-3-phosphodiester + H2O = an alcohol + sn-glycerol 3-phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12969"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:83408"/>
            <dbReference type="EC" id="3.1.4.46"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.46" evidence="1"/>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZH35"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008889">
        <property type="term" value="F:glycerophosphodiester phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006071">
        <property type="term" value="P:glycerol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08603">
        <property type="entry name" value="GDPD_SHV3_repeat_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08604">
        <property type="entry name" value="GDPD_SHV3_repeat_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.190:FF:000011">
        <property type="entry name" value="Glycerophosphodiester phosphodiesterase GDPDL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.190:FF:000013">
        <property type="entry name" value="Glycerophosphodiester phosphodiesterase GDPDL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43620:SF44">
        <property type="entry name" value="GLYCEROPHOSPHODIESTER PHOSPHODIESTERASE GDPDL6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43620">
        <property type="entry name" value="GLYCEROPHOSPHORYL DIESTER PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03009">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0319">Glycerol metabolism</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041395478" description="glycerophosphodiester phosphodiesterase" evidence="8">
        <location>
            <begin position="18"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="domain" description="GP-PDE" evidence="9">
        <location>
            <begin position="33"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="GP-PDE" evidence="9">
        <location>
            <begin position="349"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="683"/>
            <end position="724"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9688941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="749" mass="80414" checksum="2FBC2907FDB619E2" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZHA3</accession>
    <name>A0AA38ZHA3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Pectinesterase</fullName>
            <ecNumber evidence="3 8">3.1.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_014144</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6 8">
            <text>[(1-&gt;4)-alpha-D-galacturonosyl methyl ester](n) + n H2O = [(1-&gt;4)-alpha-D-galacturonosyl](n) + n methanol + n H(+)</text>
            <dbReference type="Rhea" id="RHEA:22380"/>
            <dbReference type="Rhea" id="RHEA-COMP:14570"/>
            <dbReference type="Rhea" id="RHEA-COMP:14573"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17790"/>
            <dbReference type="ChEBI" id="CHEBI:140522"/>
            <dbReference type="ChEBI" id="CHEBI:140523"/>
            <dbReference type="EC" id="3.1.1.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the pectinesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.11" evidence="3 8"/>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZHA3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030599">
        <property type="term" value="F:pectinesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042545">
        <property type="term" value="P:cell wall modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045490">
        <property type="term" value="P:pectin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000018">
        <property type="entry name" value="Pectinesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033131">
        <property type="entry name" value="Pectinesterase_Asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000070">
        <property type="entry name" value="Pectinesterase_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31321">
        <property type="entry name" value="ACYL-COA THIOESTER HYDROLASE YBHC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31321:SF57">
        <property type="entry name" value="PECTINESTERASE 53-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01095">
        <property type="entry name" value="Pectinesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00503">
        <property type="entry name" value="PECTINESTERASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0063">Aspartyl esterase</keyword>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041481762" description="Pectinesterase" evidence="8">
        <location>
            <begin position="25"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="Pectinesterase catalytic" evidence="9">
        <location>
            <begin position="88"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9688304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="380" mass="41840" checksum="2B3F4BEF4A0E57EF" modified="2024-01-24" version="1">MSKFQCVSCVVILLFLLNSTPTHCHTKGIRPKNSAGKRLSTNMTQTQFSEQQFMKWVRFVGSLRHSLFKEAKNKLFPSYTITVNKNSMAGDFRSIQDAIDSLPFVNQERVVIKVQAGVYTEKVNIPALKSFITIEGAGADKTVVQWGDTAQTIGGKGQPIGTYNSATFAVNSPYFIAKNITFKNTTPVPAPGAVGKQAVALRISGDTAAFLGCKFLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNSLSLFEGCHVHAIAQTTGALTAQGRSSLLEDTGFSFVNCKVTGSGALYLGRAWGPFSRVVFAYTYMDDIIIPKGWYNWGDPSREMTVFYGQYKCTGPGAAFAGRVSWSRELTDQEAKPFISLSYIDGSEWITLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZI25</accession>
    <name>A0AA38ZI25_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nicotianamine synthase</fullName>
            <ecNumber evidence="2">2.5.1.43</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_014927</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Synthesizes nicotianamine, a polyamine which serves as a sensor for the physiological iron status within the plant, and/or might be involved in the transport of iron.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>3 S-adenosyl-L-methionine = nicotianamine + 3 S-methyl-5'-thioadenosine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:16481"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:58249"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.5.1.43"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the nicotianamine synthase (NAS)-like family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.43" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9689481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZI25"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030410">
        <property type="term" value="F:nicotianamine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030418">
        <property type="term" value="P:nicotianamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004298">
        <property type="entry name" value="Nicotian_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32266:SF11">
        <property type="entry name" value="NICOTIANAMINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32266">
        <property type="entry name" value="NICOTIANAMINE SYNTHASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03059">
        <property type="entry name" value="NAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51142">
        <property type="entry name" value="NAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU368095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9689481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="284" mass="31694" checksum="0B0DBBD884125DEB" modified="2024-01-24" version="1">MASLQQSSLKTNVSTELLIARIVQIHANICKLESLRPSKQVNSLFTHLVKLCTPPSSIDITALPEEIQLIRQSLITLCGRAEGLLELEFSTFLTNVPQALDNLNLFPYYGNYVKLANLEYRILSDNGVVQPKKVAFIGSGPLPLTTLIMATHHMKSTCFDNFDMDESANAVALQLVASDAELQRRVKFETRDIMEVRDKLGEYDCIFLAALVGMSKEEKVKILGHVRKYMKEGGTLLVRSAKGARAFLYPVVEEEDLLGFEVLTIFHPTNEVINSVVLARKPID</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZIA0</accession>
    <name>A0AA38ZIA0_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_014985</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GLUTAMINE DUMPER 1 (TC 9.B.60) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9689580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZIA0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080143">
        <property type="term" value="P:regulation of amino acid export"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040359">
        <property type="entry name" value="GDU"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33228:SF77">
        <property type="entry name" value="PROTEIN GLUTAMINE DUMPER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33228">
        <property type="entry name" value="PROTEIN GLUTAMINE DUMPER 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="109"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="120"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="139"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9689580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="156" mass="16388" checksum="3AD40911F3A7F08A" modified="2024-01-24" version="1">MTRTGGTLNATAATPAASEQQHSPWHSPVPYLFGGLAAMLGLIAFALLILACSYWKLSGYLEGDDDASARDAEAGDGAGGDAVKPPMEFEEKILVIMAGDVKPTYLATPMSSRASSFGERSSDNSEMTEKSEAVAESPKQGSENQVQTGNPESRET</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZJR6</accession>
    <name>A0AA38ZJR6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Lysine ketoglutarate reductase trans-splicing-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_012809</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZJR6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007877">
        <property type="entry name" value="DUF707"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31210">
        <property type="entry name" value="OS06G0731900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31210:SF47">
        <property type="entry name" value="OS07G0564800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05212">
        <property type="entry name" value="DUF707"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9690345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="380" mass="44660" checksum="45DDEB1788911DDF" modified="2024-01-24" version="1">MKAFKSWRWLFRKRNAVFDGGVQGKPGFNKMKQLPFMGIVCIVMLVIVYRTTNYQYQQTEMETKFQPFGTVEGLASRTLVNLPRGIIQARSDLELRPLWSTSSSRSKVDDSSKHNLLAIPVGIKQKYNVDAIVEKFLPENFTIILFHYDGHVDGWWDLDWSSKAIHIVAQNQTKWWFAKRFLHPDVVSIYDYIFLWDEDLGVENFHPGRYLDIMKSEGLEISQPALDPNSTDIHHRITIRKRMKKFHRRVWDLRGSVKCSNISEGPPCTGFVEGMAPVFSRSAWRCTWHLIQNDLVHGWGMDMKLGYCAQVSKDAFLEETVKRQGAPAVDTRSEIRRQSTWELHVFRDRWDKAVKEDRKWVDPFKKIQRRRIPKRKSHQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZN00</accession>
    <name>A0AA38ZN00_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Chaperonin CPN60-like 2, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_011250</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the chaperonin (HSP60) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZN00"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03344">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000001">
        <property type="entry name" value="60 kDa chaperonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00600">
        <property type="entry name" value="CH60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018370">
        <property type="entry name" value="Chaperonin_Cpn60_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001844">
        <property type="entry name" value="Cpn60/GroEL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02348">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000592">
        <property type="entry name" value="PRK00013.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009487">
        <property type="entry name" value="PRK12849.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009488">
        <property type="entry name" value="PRK12850.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009489">
        <property type="entry name" value="PRK12851.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45633">
        <property type="entry name" value="60 KDA HEAT SHOCK PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00298">
        <property type="entry name" value="CHAPERONIN60"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00296">
        <property type="entry name" value="CHAPERONINS_CPN60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="554"/>
            <end position="574"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9692070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="574" mass="60948" checksum="47FFFCB708E32666" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZNI5</accession>
    <name>A0AA38ZNI5_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_011239</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZNI5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34364">
        <property type="entry name" value="WAS/WASL-INTERACTING FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34364:SF1">
        <property type="entry name" value="WAS_WASL-INTERACTING FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="17"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9692045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="181" mass="20177" checksum="6856F408A8B53C33" modified="2024-01-24" version="1">MSEGPKLYTNKPKKAQVKQFLQQQQMKGTGLSASSSPMGAQSSPPPPPPPKESFVRRYKFMWPLLLTVNLAVGAYIFMRTRKKDTGIEEEVAAAVVTTPVSTTAASTAPAPVIEKPLPSPPIMAPVKLPDPIPENQQREIFKWLLEEKRKVKPNTPEEKKRIDEEKAILKQFIRATSIPTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZNP4</accession>
    <name>A0AA38ZNP4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box/kelch-repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_011500</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZNP4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080037">
        <property type="term" value="P:negative regulation of cytokinin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000762">
        <property type="term" value="P:regulation of phenylpropanoid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044595">
        <property type="entry name" value="KMD1-4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46407">
        <property type="entry name" value="OS02G0208700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46407:SF3">
        <property type="entry name" value="OS02G0208700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9692466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="140" mass="16222" checksum="6142DC51F1104B56" modified="2024-01-24" version="1">MARERDEYKGVFHRGKFHRSAEAFDFANWEWDKAEEDFLEDRTCPRTCVDGGDMGMYMCHAGEVVALQDSRWQTVDKLPAEIRHTAYMTTWEGKLLVIGCRSLGEAHVAYMLDLKSRRWRKLVAPEEFCGHVQSGCCLEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2025-06-18" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZPC0</accession>
    <name>A0AA38ZPC0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_011532</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03233">
        <property type="entry name" value="ABCG_PDR_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03232">
        <property type="entry name" value="ABCG_PDR_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000179">
        <property type="entry name" value="ABC transporter G family member 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000059">
        <property type="entry name" value="ABC transporter G family member 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029481">
        <property type="entry name" value="ABC_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034001">
        <property type="entry name" value="ABCG_PDR_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034003">
        <property type="entry name" value="ABCG_PDR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013581">
        <property type="entry name" value="PDR_assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48040:SF20">
        <property type="entry name" value="PLEIOTROPIC DRUG RESISTANCE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48040">
        <property type="entry name" value="PLEIOTROPIC DRUG RESISTANCE PROTEIN 1-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14510">
        <property type="entry name" value="ABC_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08370">
        <property type="entry name" value="PDR_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="521"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="554"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="609"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="640"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="664"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="748"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1202"/>
            <end position="1220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1232"/>
            <end position="1249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1288"/>
            <end position="1308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1315"/>
            <end position="1339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1345"/>
            <end position="1364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1376"/>
            <end position="1395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1423"/>
            <end position="1446"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="146"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="856"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="795"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="803"/>
            <end position="812"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9692524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1454" mass="163924" checksum="9B642D5DFF570A87" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZTI4</accession>
    <name>A0AA38ZTI4_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_009871</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZTI4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05326">
        <property type="entry name" value="secoisolariciresinol-DH_like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045309">
        <property type="entry name" value="ABA2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43180">
        <property type="entry name" value="3-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE (AFU_ORTHOLOGUE AFUA_6G11210)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43180:SF41">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE REDUCTASE 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9694093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="303" mass="32473" checksum="4A279234B3B2E780" modified="2024-01-24" version="1">MPVPVMPEKPFQGLHVLGRENSSYPFYNRLEGKIAVVTGGARGIGEATVRLFARHGAKVVIADVEDTLGAALASSLAPSVTFVHCDVSLEEDIENVINSTVSRYGRLDILFNNAGVLGNQSKHKSIIDFDIDEFDQVMRVNVRGMALGIKHAARVMVPRGMGCIISTASVAGVMGGLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRSSDEEEEDCMNFGIPCEREVEKMEEFVSGLANLKGQILRAKDIAEAALYLASDESKYVSGHNLVVDGGITTSRNCVGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZUG0</accession>
    <name>A0AA38ZUG0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 14">Pyrroline-5-carboxylate reductase</fullName>
            <ecNumber evidence="4 14">1.5.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_010747</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-proline + NAD(+) = (S)-1-pyrroline-5-carboxylate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:14105"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17388"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="EC" id="1.5.1.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 14">
            <text>L-proline + NADP(+) = (S)-1-pyrroline-5-carboxylate + NADPH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:14109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17388"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="EC" id="1.5.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Amino-acid biosynthesis; L-proline biosynthesis; L-proline from L-glutamate 5-semialdehyde: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the pyrroline-5-carboxylate reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.2" evidence="4 14"/>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZUG0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004735">
        <property type="term" value="F:pyrroline-5-carboxylate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3730.10:FF:000001">
        <property type="entry name" value="Pyrroline-5-carboxylate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000190">
        <property type="entry name" value="Pyrroline-5-carboxylate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3730.10">
        <property type="entry name" value="ProC C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01925">
        <property type="entry name" value="P5C_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028939">
        <property type="entry name" value="P5C_Rdtase_cat_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053790">
        <property type="entry name" value="P5CR-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029036">
        <property type="entry name" value="P5CR_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000304">
        <property type="entry name" value="Pyrroline-COOH_reductase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00112">
        <property type="entry name" value="proC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11645">
        <property type="entry name" value="PYRROLINE-5-CARBOXYLATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11645:SF0">
        <property type="entry name" value="PYRROLINE-5-CARBOXYLATE REDUCTASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03807">
        <property type="entry name" value="F420_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14748">
        <property type="entry name" value="P5CR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000193">
        <property type="entry name" value="Pyrrol-5-carb_rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00521">
        <property type="entry name" value="P5CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 13 14" id="KW-0521">NADP</keyword>
    <keyword evidence="10 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8 14" id="KW-0641">Proline biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyrroline-5-carboxylate reductase catalytic N-terminal" evidence="15">
        <location>
            <begin position="13"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="Pyrroline-5-carboxylate reductase dimerisation" evidence="16">
        <location>
            <begin position="169"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="17"/>
            <end position="22"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>NADPH</name>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00050547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00052690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000193-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF03807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF14748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9695432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="276" mass="28817" checksum="94F0826B59C3E875" modified="2024-01-24" version="1">MAGVAPIPMDTFKLGFIGAGKMAESIAKGVVHSGLMPASRISTAPRSSGRQVAFESFGVKIFETNQRVVEDSDVIVFSVKPQVVKDVVLELKPLLTKKKLLVSVVAGVKLKHLQEWACHSRFIRVVPNTPAAVGKAATVMSLGKSATEEDGGLIANLFGAIGKIWRADEKYFDAVTGLSGSGPAYIYIAIEALADGGVAAGLPRELAWNLASQTVLGAADMAISTGKHPGQLKDDVASPGGTTIAGIYELEKGGFRGILMNAVVAAAKRSLDLSES</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZWE6</accession>
    <name>A0AA38ZWE6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Sodium/calcium exchanger membrane region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_008647</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZWE6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030001">
        <property type="term" value="P:metal ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="entry name" value="NCX, central ion-binding region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051171">
        <property type="entry name" value="CaCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11878:SF65">
        <property type="entry name" value="NA_CA-EXCHANGE PROTEIN, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11878">
        <property type="entry name" value="SODIUM/CALCIUM EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="94"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="138"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="168"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="195"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="339"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="368"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="401"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="437"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="479"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="513"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="8">
        <location>
            <begin position="45"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="8">
        <location>
            <begin position="370"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9696528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="541" mass="60272" checksum="84B482F6F5084696" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZWN4</accession>
    <name>A0AA38ZWN4_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_008439</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZWN4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010333">
        <property type="term" value="F:terpene synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009686">
        <property type="term" value="P:gibberellin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.160:FF:000001">
        <property type="entry name" value="Ent-copalyl diphosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.600.10:FF:000035">
        <property type="entry name" value="Ent-copalyl diphosphate synthase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.130:FF:000002">
        <property type="entry name" value="Ent-copalyl diphosphate synthase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.130">
        <property type="entry name" value="Terpene synthase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001906">
        <property type="entry name" value="Terpene_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036965">
        <property type="entry name" value="Terpene_synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050148">
        <property type="entry name" value="Terpene_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005630">
        <property type="entry name" value="Terpene_synthase_metal-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31739">
        <property type="entry name" value="ENT-COPALYL DIPHOSPHATE SYNTHASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31739:SF4">
        <property type="entry name" value="ENT-COPALYL DIPHOSPHATE SYNTHASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01397">
        <property type="entry name" value="Terpene_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03936">
        <property type="entry name" value="Terpene_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01014">
        <property type="entry name" value="Terpene_Cyclase_Like_1_N-term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01605">
        <property type="entry name" value="Terpene_Cyclase_Like_1_N-term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Terpene synthase N-terminal" evidence="4">
        <location>
            <begin position="280"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="domain" description="Terpene synthase metal-binding" evidence="5">
        <location>
            <begin position="561"/>
            <end position="694"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9696192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1061" mass="121358" checksum="BDB4EBB4186784A9" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZX11</accession>
    <name>A0AA38ZX11_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Germin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_008819</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Secreted</location>
            <location evidence="1 13">Extracellular space</location>
            <location evidence="1 13">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the germin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZX11"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009506">
        <property type="term" value="C:plasmodesma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010497">
        <property type="term" value="P:plasmodesmata-mediated intercellular transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000280">
        <property type="term" value="P:regulation of root development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02241">
        <property type="entry name" value="cupin_OxOx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000025">
        <property type="entry name" value="germin-like protein subfamily 2 member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006045">
        <property type="entry name" value="Cupin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001929">
        <property type="entry name" value="Germin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019780">
        <property type="entry name" value="Germin_Mn-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31238">
        <property type="entry name" value="GERMIN-LIKE PROTEIN SUBFAMILY 3 MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00190">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00325">
        <property type="entry name" value="GERMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00835">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00725">
        <property type="entry name" value="GERMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 13" id="KW-0052">Apoplast</keyword>
    <keyword evidence="7 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 10 13" id="KW-0464">Manganese</keyword>
    <keyword evidence="5 10 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041483706" description="Germin-like protein" evidence="13">
        <location>
            <begin position="20"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="Cupin type-1" evidence="14">
        <location>
            <begin position="58"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="29"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU366015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9696796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="217" mass="22650" checksum="C1E89D11BC44ADD6" modified="2024-01-24" version="1">METLFAYLLIFAAISGALASDPDLLQDVCVADFASGTKLNGYACKDAANISAADFFFDGLAKAGVPNNLMGSVVTAANVQKISGLNTLGVSLSRVDIAPGGLNPPHTHPRATELAFVLAGVLDVGFITASNVLVSKTIKEGEVFVFPRGLIHFQKNNGDKPAAILGAFNSQLPGTQSIAATLFTATPEVQDNVLTKAFQIGTKEVEKMKSKLAPKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZY03</accession>
    <name>A0AA38ZY03_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_008967</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the mTERF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZY03"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.70.10:FF:000001">
        <property type="entry name" value="Mitochondrial transcription termination factor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.70.10">
        <property type="entry name" value="Transcription termination factor 3, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003690">
        <property type="entry name" value="MTERF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038538">
        <property type="entry name" value="MTERF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13068">
        <property type="entry name" value="CGI-12 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13068:SF133">
        <property type="entry name" value="MITOCHONDRIAL TRANSCRIPTION TERMINATION FACTOR FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02536">
        <property type="entry name" value="mTERF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00733">
        <property type="entry name" value="Mterf"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0806">Transcription termination</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9696995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="387" mass="43519" checksum="3FAF86DF81194EF8" modified="2024-01-24" version="1">MCIKFISKRIGLIPKMWQAASITQLHFLRNSTPCIFRSFSASNQHSFTVSYLVNSCGLSPETAISASGKIRFENPKNPDSVLALLRNSGCTNTHITKIVTKLPSLLLVNPEKTLLPKLEFFRSMDLSGADLASILSSRPSILRKSLKKVLIPKYNFLKSLNISNEDAVKVLKRSSWSSSGNLERTIAANIAVLREIGVPISHISFLVARYHSIGQRSDKFSENVKAVVEMGFNPLKFTFLNALQSFCQMTESTRQQKMEMYRRWGWSEDEILLAFRTCPQCMQLSESKVTKVLDFLVNKMGWQPAVVARAPIALCLNFEKRVVPRCSVVKVLLLKGLVKKDLKLGHFLSVTEGDFVDKYVIKNLDDIPQLLDLYQGKVSFMGLEFGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A4W9</accession>
    <name>A0AA39A4W9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">alpha-1,2-Mannosidase</fullName>
            <ecNumber evidence="7">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_006034</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the glycosyl hydrolase 47 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="7"/>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A4W9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044322">
        <property type="term" value="C:endoplasmic reticulum quality control compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004571">
        <property type="term" value="F:mannosyl-oligosaccharide 1,2-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904380">
        <property type="term" value="P:endoplasmic reticulum mannose trimming"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000015">
        <property type="entry name" value="alpha-1,2-Mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044674">
        <property type="entry name" value="EDEM1/2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001382">
        <property type="entry name" value="Glyco_hydro_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036026">
        <property type="entry name" value="Seven-hairpin_glycosidases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45679">
        <property type="entry name" value="ER DEGRADATION-ENHANCING ALPHA-MANNOSIDASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45679:SF6">
        <property type="entry name" value="ER DEGRADATION-ENHANCING ALPHA-MANNOSIDASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01532">
        <property type="entry name" value="Glyco_hydro_47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00747">
        <property type="entry name" value="GLYHDRLASE47"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48225">
        <property type="entry name" value="Seven-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041305328" description="alpha-1,2-Mannosidase" evidence="9">
        <location>
            <begin position="28"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="505"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="611"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="510"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="620"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="632"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="120"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="260"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="353"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="374"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="464"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9700527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="649" mass="72742" checksum="29A154AA7C4E7336" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A4Z1</accession>
    <name>A0AA39A4Z1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">CRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_006050</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9700557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A4Z1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000373">
        <property type="term" value="P:Group II intron splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.110.60:FF:000003">
        <property type="entry name" value="CRM-domain containing factor CFM3B, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.110.60:FF:000002">
        <property type="entry name" value="CRS2-associated factor 1, chloroplastic"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.60">
        <property type="entry name" value="YhbY-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045278">
        <property type="entry name" value="CRS1/CFM2/CFM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001890">
        <property type="entry name" value="RNA-binding_CRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035920">
        <property type="entry name" value="YhbY-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31846:SF19">
        <property type="entry name" value="CRM-DOMAIN CONTAINING FACTOR CFM3A, CHLOROPLASTIC_MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31846">
        <property type="entry name" value="CRS1 / YHBY (CRM) DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01985">
        <property type="entry name" value="CRS1_YhbY"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01103">
        <property type="entry name" value="CRS1_YhbY"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75471">
        <property type="entry name" value="YhbY-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51295">
        <property type="entry name" value="CRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="3" id="KW-0934">Plastid</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="CRM" evidence="13">
        <location>
            <begin position="218"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="CRM" evidence="13">
        <location>
            <begin position="430"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="CRM" evidence="13">
        <location>
            <begin position="643"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="87"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="854"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="598"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="763"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="854"/>
            <end position="872"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9700557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="902" mass="101750" checksum="D5CBFD6E1EE9BDA0" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A576</accession>
    <name>A0AA39A576_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_006517</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A576"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080043">
        <property type="term" value="F:quercetin 3-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080044">
        <property type="term" value="F:quercetin 7-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926">
        <property type="entry name" value="GLUCOSYL/GLUCURONOSYL TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926:SF1542">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="27"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="27"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9701208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="241" mass="27297" checksum="C4908ED650010A2D" modified="2024-01-24" version="1">MESVQLEHENVTELVLVTLRDGVEEGRRFSNKEKDPKTSKELNNEEKDSMTSKELNEEPYEVKISSTGVRMLNKHYLFRAPPNANADNFVLRAFQKHLNLLHHDTNPTALINTFDALEPEALRAVTEFNSIGVGPLFPTAFLGGKDPSDTSFGGDLFRRSKDYIEWLNTNPESSVIYVSFGSLEVLSKQQSEEIARGLLDSGRPFLWVIRAKEKGEEEKEEDKLSCLEELERQGMMVPSCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A5M3</accession>
    <name>A0AA39A5M3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_006581</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A5M3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF349">
        <property type="entry name" value="AMINO ACID TRANSPORTER TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="101"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="125"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="157"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="204"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="237"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="280"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="331"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="357"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="391"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="8">
        <location>
            <begin position="20"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9701296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="425" mass="45995" checksum="19DCCE9F16EA5F4F" modified="2024-01-24" version="1">MEAHGKSAETPLLGTSHRGTASSIQTLGNILVSIVGTGVLGLPFAFRVAGWLAGTVGVIITGLSTCYCMLILVQCRKRLVCEEEKTYGDLGYECLGKPGRYLTEFLIFISYCGGSVAYLKFIGQTLASVFSGMTFTSFIFCLVPIEIMLSWIRTLSALSPFSLFADVCNIAAIAMVVKEDVQVLCGSGSDIGERRALSPTISGLPFGAGVAVFCFEGFGMTLALEGSMRERGAFTRVLIQAFCGLTSVYVLFGLIGYLAYGDQTLDIVTLNLPQGWPSMVVQLGLCMGLVFTLPIMLHPLHEIIEVKLKESRWFQRYCYDGGDYLTRAGKLVMYIMRAVVVMELALVASYVPAFGVFTSLVGSTVCSLISFVLPTIFHLKISGSSLPTWQKALDVCILSGGLLFACYGTYNTIFGVPRDLGYIHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A717</accession>
    <name>A0AA39A717_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF4283 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_006426</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A717"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="372"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="492"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="643"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="394"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="645"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="678"/>
            <end position="692"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9701068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="780" mass="86415" checksum="EB4CB64B20E8A61F" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A808</accession>
    <name>A0AA39A808_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Hydroxyproline O-arabinosyltransferase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_004421</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A808"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056508">
        <property type="entry name" value="HPAT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044845">
        <property type="entry name" value="HPAT/SRGT1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31485:SF3">
        <property type="entry name" value="HYDROXYPROLINE O-ARABINOSYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31485">
        <property type="entry name" value="PEPTIDYL SERINE ALPHA-GALACTOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23452">
        <property type="entry name" value="HPAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="domain" description="Hydroxyproline O-arabinosyltransferase-like" evidence="8">
        <location>
            <begin position="70"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9702681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="364" mass="41176" checksum="B8E974930CB4DDC9" modified="2024-01-24" version="1">MGCGNFFYVLLITFSVALITYNILISANAPLKQGFPGNPSSSSTPFSVDPIIKMPIDRSNPTSTKGKRLFHTAVTASDSIYNTWQCRVMYYWFKKFKDGPNSEMGGFTRILHSGKPDKFMHEIPTFVAQPLPAGMDQGYIVLNRPWAFVQWLQQADIKEDYILMAEPDHVIVKPIPNLSRDGLGAAFPFFYIQPKKYESTLRKFYPEEKGPITNVDPIGNSPVIVGKESLKKIAPTWMNVSLAMKKDPEADKTFGWVLEMYAYAVASALHDVGNILYKDFMIQPPWDTEIGKKFIIHYTYGCDYDMQGKLTYGKIGEWRFDKRSFDSKWPPRNLPLPPPGVPESVVTLVKMVNEATANIPNWGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A8K1</accession>
    <name>A0AA39A8K1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Glycosyltransferase 2-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_004562</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the glycosyltransferase 2 family. Plant cellulose synthase-like C subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A8K1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099402">
        <property type="term" value="P:plant organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048868">
        <property type="term" value="P:pollen tube development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000007">
        <property type="entry name" value="probable xyloglucan glycosyltransferase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32044">
        <property type="entry name" value="GLUCOMANNAN 4-BETA-MANNOSYLTRANSFERASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32044:SF101">
        <property type="entry name" value="GLYCOSYLTRANSFERASE 2-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13632">
        <property type="entry name" value="Glyco_trans_2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="64"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="107"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="488"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="670"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="11">
        <location>
            <begin position="322"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF13632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9702871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="694" mass="79472" checksum="F75AC03104281DAF" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ABH5</accession>
    <name>A0AA39ABH5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WAT1-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_005302</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the drug/metabolite transporter (DMT) superfamily. Plant drug/metabolite exporter (P-DME) (TC 2.A.7.4) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ABH5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030184">
        <property type="entry name" value="WAT1-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31218">
        <property type="entry name" value="WAT1-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="29"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="84"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="170"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="201"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="239"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="264"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="291"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="2"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="171"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9703962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="374" mass="40261" checksum="3B868521EFC18C64" modified="2024-01-24" version="1">MVIVQIIFGGLNIMYKLARNDGMSMKILVAYRNIFATAIMVPLALIFEGKSRPKLTWMIFLQGSLCGLFGGSLGQNLYAESLNLTSATFVAAMTNLVPAMTFVMAVFLRMERLAIGTIAGKAKVLGTIMSLGGAMILTFYKGVEIKLWSTNINLLHHGAAALQESSPNQVLGSLLAVAGCVCIAVWLIVQTKMSMVYPSYSGTALMCVCASIQSVVYAMCTERDWSAWKLGWNIRLLTVVYSGVLASGLMVTLMTWVSRMRGPLFVSSFFPLMLVTVAILGSLLLHEQLHIGSIIAAVLIIVGLYIVLWGKGKEMKQTAQIHAAQSFSEQDLRHIVIENSSNSECKPKASGIIGPIEPADLGSGLPSAAAVVSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ADR4</accession>
    <name>A0AA39ADR4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_003625</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ADR4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33710">
        <property type="entry name" value="BNAC02G09200D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33710:SF71">
        <property type="entry name" value="ENDONUCLEASE_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="403"/>
            <end position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9705647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="532" mass="61899" checksum="B8A20011DD31AFB2" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AEK6</accession>
    <name>A0AA39AEK6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phytocyanin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_003153</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AEK6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003245">
        <property type="entry name" value="Phytocyanin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34052">
        <property type="entry name" value="GLYCINE-RICH PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34052:SF1">
        <property type="entry name" value="OS06G0216700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02298">
        <property type="entry name" value="Cu_bind_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51485">
        <property type="entry name" value="PHYTOCYANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041437576" description="Phytocyanin domain-containing protein" evidence="1">
        <location>
            <begin position="26"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="Phytocyanin" evidence="2">
        <location>
            <begin position="66"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9704968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="185" mass="20920" checksum="21563EAF0A523F50" modified="2024-01-24" version="1">MGFTSARGVLTLLVLTASMLAVGLASRPIAGRPANYWNFGFNHSFWGPRNGHLFHPNNDNTTRPPKKFIVGGSEHWRYGFNYTDWALNNGPFYVNDTLVFKYDPPSKTTFPHSVYLLPNPWSFLTCDLSKAEQVATVAQGSGEGFEFVLKNWWPYYFACGEHNGLHCKEGMMKFFVMPLLGPYHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AEQ9</accession>
    <name>A0AA39AEQ9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_003204</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AEQ9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14707">
        <property type="entry name" value="bZIP_plant_BZIP46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000036">
        <property type="entry name" value="ABSCISIC ACID-INSENSITIVE 5-like protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043452">
        <property type="entry name" value="BZIP46-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22952:SF463">
        <property type="entry name" value="ABSCISIC ACID-INSENSITIVE 5-LIKE PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22952">
        <property type="entry name" value="CAMP-RESPONSE ELEMENT BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BZIP" evidence="6">
        <location>
            <begin position="348"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="197"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="369"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9705043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="438" mass="47229" checksum="CB8295B27F0DD0D5" modified="2024-01-24" version="1">MNFKNFSDMPPEGNGGKPMGNFSLTRQPSIYSLTIDEFQNSLGGVGKDFGSMNMDELLKNIWTAEETQNMTSSAGGEGSVPGGNLQRQGSLTLPRTISQKTVDEVWKDLLKENSALKEGSVGGPPNLQQRQPTLGEMTLEEFLVRVGVVREDAQPTVRPNNSGFYGLSSQPNHAGLALGFQQSGRNNGALTNRITESNYLVPNQPPSLALNMSGARSSQQQQQDHHQQQQQQQQPLFPKQATVAFSSPMHLTNTTQLASSGVRGAVVGIANPTVNNGLVPNGGLQSGGMAMIGLGTGAVLGASGSPANHISSDVIGKSNVNTSSLSPAPYVFNGSQRGRKPGALEKVVERRQRRMIKNRESAARSRARKQAYTLELEMEVAKLKEANEELQKKQADMEVQKNQVQALFSCAVASVLSLHWQTPILIEDNVYIVPLKAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AFF1</accession>
    <name>A0AA39AFF1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Thaumatin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_001887</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thaumatin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AFF1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.110.10:FF:000003">
        <property type="entry name" value="Thaumatin I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.110.10">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037176">
        <property type="entry name" value="Osmotin/thaumatin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001938">
        <property type="entry name" value="Thaumatin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31048">
        <property type="entry name" value="OS03G0233200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00314">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002703">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00347">
        <property type="entry name" value="THAUMATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00205">
        <property type="entry name" value="THN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49870">
        <property type="entry name" value="Osmotin, thaumatin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51367">
        <property type="entry name" value="THAUMATIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041339642" description="Thaumatin-like protein" evidence="4">
        <location>
            <begin position="29"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="37"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="77"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="92"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="143"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="148"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="156"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="170"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="180"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR002703-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9706638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="227" mass="24520" checksum="2EF97C81BBEDE3F2" modified="2024-01-24" version="1">MSFFCNCSNLSTSFCLLILPFFFTFIHAATLEIRNSCPYTVWAAAVPGGGWQLDQGQTWNLSVSSNTKGRIWGRTKCSFDKAGRGSCESGGCGGLLQCEDSGSPPNTLAEYALNQINNTDFFDISLVDGFNIPMEFSPTSDGCRGANCKGDINGPCPNELRDPGGCNNPCTVFNNNQYCCTSGSCGPTTYSEFFKDRCPNAYSYPKDDATSTFTCSGGSNYRVVFCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AGY9</accession>
    <name>A0AA39AGY9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Fungal lipase-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_002121</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AGY9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047714">
        <property type="term" value="F:galactolipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008970">
        <property type="term" value="F:phospholipase A1 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00519">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002921">
        <property type="entry name" value="Fungal_lipase-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31403">
        <property type="entry name" value="PHOSPHOLIPASE A1-IBETA2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31403:SF2">
        <property type="entry name" value="PHOSPHOLIPASE A1-IBETA2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01764">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4" id="KW-0934">Plastid</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Fungal lipase-type" evidence="10">
        <location>
            <begin position="255"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="64"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9706999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="528" mass="58909" checksum="AB5FDD5BDF3806D0" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AHD6</accession>
    <name>A0AA39AHD6_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_002296</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AHD6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35297">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35297:SF2">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="61"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9707239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="147" mass="16533" checksum="73983C771956D04E" modified="2024-01-24" version="1">MQRQSLGSPSSKLHIHGGGGGGAKEEILKAEKDLNQSQNDDEADHKSLKPLRSSSSPPDKLIHLIPLLMLFCFLLLYLFSHDPSHSELAQFDGFKKRSSKLTDSNEIVDLERFVVKSDVFSIRSLRNLQEIGTYAPKSRLHRKMGDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AJ32</accession>
    <name>A0AA39AJ32_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_000513</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AJ32"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000234">
        <property type="entry name" value="Probable inactive receptor kinase RLK902"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000095">
        <property type="entry name" value="protein STRUBBELIG-RECEPTOR FAMILY 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050994">
        <property type="entry name" value="At_inactive_RLKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48010:SF76">
        <property type="entry name" value="INACTIVE RECEPTOR KINASE RLK902-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48010">
        <property type="entry name" value="OS05G0588300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041208721" description="Protein kinase domain-containing protein" evidence="11">
        <location>
            <begin position="21"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="229"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="320"/>
            <end position="590"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9708512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="615" mass="67503" checksum="8AA7A65B1A46CE7F" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AJR0</accession>
    <name>A0AA39AJR0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">PVL29_000728</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="24">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PC-esterase family. TBL subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AJR0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048638">
        <property type="term" value="P:regulation of developmental growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000021">
        <property type="entry name" value="Putative LRR receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000015">
        <property type="entry name" value="Somatic embryogenesis receptor kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000016">
        <property type="entry name" value="Somatic embryogenesis receptor-like kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026057">
        <property type="entry name" value="TBL_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47988">
        <property type="entry name" value="SOMATIC EMBRYOGENESIS RECEPTOR KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13839">
        <property type="entry name" value="PC-Esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="15 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9 22" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041232353" description="non-specific serine/threonine protein kinase" evidence="22">
        <location>
            <begin position="25"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="239"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="303"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="331"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KAJ9708872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="920" mass="103771" checksum="47D6A31861B28FDD" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AL93</accession>
    <name>A0AA39AL93_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_000356</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AL93"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01745">
        <property type="entry name" value="GATase1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011697">
        <property type="entry name" value="Peptidase_C26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044668">
        <property type="entry name" value="PuuD-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43235">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE PB2B2.05-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43235:SF1">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE PB2B2.05-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07722">
        <property type="entry name" value="Peptidase_C26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9708254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="425" mass="48564" checksum="9F78F527F43795E4" modified="2024-01-24" version="1">MASDISTIFPRVLIVSRRTVRKNKFVDFVGEYHLDLIVNYGAVPVIVPRVSGVHMLLESFEPIHGVLLCEGEDIDPSLYDAELSGFSPEEIEEIRRLHASDTAIDREKDSIELLLAKLCLERSIPFLGICRGSQVLNVACGGSLYQDVERELSKKCPEGQRVVHMNYENYDGHRHIVKVVENTPLHHWFKDSLEANKMDIWVNSYHHQGVKRLAQRFVPLAFAPDGLVEGFYDPHVYNPEEGNFIMGLQFHPERMRQPNSDDFDYPGCPSAYQEFVKAVKAYQKKLNGSAFVAKTLKLDEDVEKKRKVIARSFSIARDMYSTRRGMIGEEESELEVGAKFLEANTALSLQQEKRLKQMGATVRNASVYMERLKMNEEKEKLARTMMGKMSIEQLSEIVSFYHMMGRICSEVLERKLHNLTNEIGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AML9</accession>
    <name>A0AA39AML9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">LITAF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_001437</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CDIP1/LITAF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AML9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006629">
        <property type="entry name" value="LITAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037519">
        <property type="entry name" value="LITAF_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23292:SF6">
        <property type="entry name" value="FI16602P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23292">
        <property type="entry name" value="LIPOPOLYSACCHARIDE-INDUCED TUMOR NECROSIS FACTOR-ALPHA FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10601">
        <property type="entry name" value="zf-LITAF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00714">
        <property type="entry name" value="LITAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51837">
        <property type="entry name" value="LITAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="LITAF" evidence="7">
        <location>
            <begin position="38"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9709954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="141" mass="15558" checksum="D13917FC821171E4" modified="2024-01-24" version="1">MAKKEEEPVMGIPYPAVYHPNQNPYQAGVIPPNAFVGDPKGVPIQQTIFRDTPAPFNCVYCGSSGVTTVKSKLSLAAVVGCMMPMMLGICFLCPSMDCLWHKYHYCPSCKEKVADFEKSDPCVVMDPPQWTQQSFALLASY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D495</accession>
    <name>A0AA39D495_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Isoflavone 2'-hydroxylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_026854</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D495"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20653">
        <property type="entry name" value="CYP81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000023">
        <property type="entry name" value="Cytochrome P450 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050651">
        <property type="entry name" value="Plant_Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47947">
        <property type="entry name" value="CYTOCHROME P450 82C3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47947:SF62">
        <property type="entry name" value="CYTOCHROME P450, FAMILY 81, SUBFAMILY D, POLYPEPTIDE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11 12" id="KW-0349">Heme</keyword>
    <keyword evidence="8 11 12" id="KW-0408">Iron</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 12" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="11">
        <location>
            <position position="441"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9670571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="502" mass="56854" checksum="4175AAB528DE1DAF" modified="2024-01-24" version="1">MEVSSLHTHLCLLFVFLLLAFGIFFPRRRRYGNLPPSPPAVPIIGHLHLLKHPVHRSLQRLSQKYGPIFSLRFGSQLAVIVSSPSAVEECFTKNDVVLADRPRLASGKYVGFNYTNITAASYGEHWRNLRRISALEIFSSNRLNMFLGIRRDEVKRLLLRLARDSREGFAKVEMRPMLTELNFNIITRMVAGKRYYGEDLEYAEAKRFRDIISEIFELLGALANPADFLPILRWIGLGYHEKKLKKIMRETKAILQGLIDEHRSGNDKGSVDNNSMIDHLLSLQKTEPEYYTDDIIKGLVLVLILGGTDTSAATMEWAMALLLNHPDVLEKAKVELDMHVGKDRLIEESDLPKLRYLQSIISETMRAFPVGPLLVPHMSSDDCQIGGFDIPRGTILLVNAWALHRDPQVWEDPTSFKPERFENGEREDYKLVPFGIGRRACPGAGLAQRVVGLALGSLIQCYDWKKISNTAIDTTEGKGLSMPKLEPLEAMCKAREIINQVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D6Y0</accession>
    <name>A0AA39D6Y0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">4-coumarate--CoA ligase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_023135</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D6Y0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016405">
        <property type="term" value="F:CoA-ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05904">
        <property type="entry name" value="4CL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000003">
        <property type="entry name" value="Long-chain-fatty-acid--CoA ligase FadD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096:SF413">
        <property type="entry name" value="PEROXISOMAL OPC-8:0-COA LIGASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="114"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="271"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="4">
        <location>
            <begin position="73"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="5">
        <location>
            <begin position="485"/>
            <end position="538"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9673376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="554" mass="59735" checksum="E1401C78E11BBC11" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D8P8</accession>
    <name>A0AA39D8P8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">TIR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_024805</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the disease resistance TIR-NB-LRR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D8P8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050832">
        <property type="term" value="P:defense response to fungus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043068">
        <property type="term" value="P:positive regulation of programmed cell death"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10140:FF:000007">
        <property type="entry name" value="Disease resistance protein (TIR-NBS-LRR class)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011713">
        <property type="entry name" value="Leu-rich_rpt_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058192">
        <property type="entry name" value="WHD_ROQ1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017:SF479">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN (TIR-NBS-LRR CLASS) FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07725">
        <property type="entry name" value="LRR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23282">
        <property type="entry name" value="WHD_ROQ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0611">Plant defense</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="TIR" evidence="11">
        <location>
            <begin position="12"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="994"/>
            <end position="1015"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9675998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1026" mass="115831" checksum="76D958CEE719E6B4" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D8S9</accession>
    <name>A0AA39D8S9_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_024873</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9676088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D8S9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9676088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="110" mass="12250" checksum="55BF1ACC8C4FC070" modified="2024-01-24" version="1">MPLPETVGPVARNKWELVMVPFQLANGGLVRGCLLVQFPQLMCGALLMGKGLKNSPLIHCAGNIMHPHPHRHLQWTLRLLMVCACELCFVAAPMPAQCIINRIREATNWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D9M7</accession>
    <name>A0AA39D9M7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">Laccase</fullName>
            <ecNumber evidence="4 13">1.10.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Benzenediol:oxygen oxidoreductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Diphenol oxidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Urishiol oxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_024312</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Lignin degradation and detoxification of lignin-derived products.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>4 hydroquinone + O2 = 4 benzosemiquinone + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:11276"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17594"/>
            <dbReference type="ChEBI" id="CHEBI:17977"/>
            <dbReference type="EC" id="1.10.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="13">Binds 4 Cu cations per monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Secreted</location>
            <location evidence="2 13">Extracellular space</location>
            <location evidence="2 13">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the multicopper oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.10.3.2" evidence="4 13"/>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D9M7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052716">
        <property type="term" value="F:hydroquinone:oxygen oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046274">
        <property type="term" value="P:lignin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13849">
        <property type="entry name" value="CuRO_1_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13875">
        <property type="entry name" value="CuRO_2_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13897">
        <property type="entry name" value="CuRO_3_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002355">
        <property type="entry name" value="Cu_oxidase_Cu_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034288">
        <property type="entry name" value="CuRO_1_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034285">
        <property type="entry name" value="CuRO_2_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034289">
        <property type="entry name" value="CuRO_3_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017761">
        <property type="entry name" value="Laccase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03389">
        <property type="entry name" value="laccase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF410">
        <property type="entry name" value="LACCASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00080">
        <property type="entry name" value="MULTICOPPER_OXIDASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0052">Apoplast</keyword>
    <keyword evidence="10 13" id="KW-0186">Copper</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 13" id="KW-0439">Lignin degradation</keyword>
    <keyword evidence="7 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 13" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Plastocyanin-like" evidence="16">
        <location>
            <begin position="39"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="14">
        <location>
            <begin position="165"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="15">
        <location>
            <begin position="417"/>
            <end position="551"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9675310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="568" mass="63745" checksum="2C372F2F61954CA3" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D9P5</accession>
    <name>A0AA39D9P5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase</fullName>
            <ecNumber evidence="1">3.2.2.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_024683</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>NAD(+) + H2O = ADP-D-ribose + nicotinamide + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16301"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57967"/>
            <dbReference type="EC" id="3.2.2.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:16302"/>
        </physiologicalReaction>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.6" evidence="1"/>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D9P5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061809">
        <property type="term" value="F:NAD+ nucleosidase activity, cyclic ADP-ribose generating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045344">
        <property type="entry name" value="C-JID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017:SF570">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN (TIR-NBS CLASS)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20160">
        <property type="entry name" value="C-JID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="68"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9675849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="740" mass="85467" checksum="D4D11F0017073ACE" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D9W6</accession>
    <name>A0AA39D9W6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_024854</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9676059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D9W6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04051">
        <property type="entry name" value="C2_SRC2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044750">
        <property type="entry name" value="C2_SRC2/BAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32246:SF22">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32246">
        <property type="entry name" value="INGRESSION PROTEIN FIC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="1">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9676059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="194" mass="21400" checksum="5201FDFC987468A4" modified="2024-01-24" version="1">MEWRMFEITLISARNLEVRETQKTNVYAKISIAGHPITEKRTPVDKKGGANPAWNFTTTCMIGKQAVEHDVVKIEIKLYRSRALGDKYIGKVCVSFKELFNLERTLWGRLRTLFSCPKGAGVGKTVKYSVKKNGGSDSSGVLKFSYRFGEVVIVNQPSGQGSTSPLVAELVVLFIKVVIAAALSIDIPFSWAGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D9Y3</accession>
    <name>A0AA39D9Y3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Threonylcarbamoyladenosine tRNA methylthiotransferase</fullName>
            <ecNumber evidence="4">2.8.4.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">tRNA-t(6)A37 methylthiotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PVL29_024526</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the methylthiolation of N6-threonylcarbamoyladenosine (t(6)A), leading to the formation of 2-methylthio-N6-threonylcarbamoyladenosine (ms(2)t(6)A) at position 37 in tRNAs that read codons beginning with adenine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-SH + AH2 + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-H + 5'-deoxyadenosine + L-methionine + A + S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:37075"/>
            <dbReference type="Rhea" id="RHEA-COMP:10163"/>
            <dbReference type="Rhea" id="RHEA-COMP:11092"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="ChEBI" id="CHEBI:74418"/>
            <dbReference type="ChEBI" id="CHEBI:74420"/>
            <dbReference type="EC" id="2.8.4.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the methylthiotransferase family. CDKAL1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.8.4.5" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D9Y3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035598">
        <property type="term" value="F:tRNA (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-methylthiotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12160:FF:000009">
        <property type="entry name" value="threonylcarbamoyladenosine tRNA methylthiotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.30.20:FF:000002">
        <property type="entry name" value="threonylcarbamoyladenosine tRNA methylthiotransferase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12160">
        <property type="entry name" value="Methylthiotransferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="entry name" value="tm_1862 like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005839">
        <property type="entry name" value="Methylthiotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020612">
        <property type="entry name" value="Methylthiotransferase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013848">
        <property type="entry name" value="Methylthiotransferase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038135">
        <property type="entry name" value="Methylthiotransferase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006466">
        <property type="entry name" value="MiaB-like_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01578">
        <property type="entry name" value="MiaB-like-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00089">
        <property type="entry name" value="MiaB/RimO family radical SAM methylthiotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11918">
        <property type="entry name" value="RADICAL SAM PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11918:SF45">
        <property type="entry name" value="THREONYLCARBAMOYLADENOSINE TRNA METHYLTHIOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00919">
        <property type="entry name" value="UPF0004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01082">
        <property type="entry name" value="B12-binding_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51449">
        <property type="entry name" value="MTTASE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01278">
        <property type="entry name" value="MTTASE_RADICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0819">tRNA processing</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="583"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="domain" description="MTTase N-terminal" evidence="17">
        <location>
            <begin position="42"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="18">
        <location>
            <begin position="171"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="TRAM" evidence="16">
        <location>
            <begin position="404"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00051661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ9675640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="605" mass="66656" checksum="9003A9DC616D7B06" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DC63</accession>
    <name>A0AA39DC63_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_022932</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9678190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DC63"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9678190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="151" mass="17860" checksum="CE4924BD4F75D78D" modified="2024-01-24" version="1">MEVQAFYTTGILKLTSPSMKCIHFKYMDHKFMYCKCKHLKCMHLVFLLVFNLDYCLKAKENLLFYQCRKLVDNLHVQDNPPIKAPSNWVEQTSVGRRYYYNKRIRQSKNLHNLFGSLDRSLIKEVKVKEMEENEDKPTLAMITDANVSCNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DEM2</accession>
    <name>A0AA39DEM2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cytochrome P450 704C1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_020022</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DEM2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11064">
        <property type="entry name" value="CYP86A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24296">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0349">Heme</keyword>
    <keyword evidence="6 8" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="8">
        <location>
            <position position="467"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9680890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="530" mass="60919" checksum="F716B8CC0606CFBD" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DET6</accession>
    <name>A0AA39DET6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_021502</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DET6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000590">
        <property type="entry name" value="PR5-like receptor kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000178">
        <property type="entry name" value="serine/threonine-protein kinase PBS1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045874">
        <property type="entry name" value="LRK10/LRL21-25-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025287">
        <property type="entry name" value="WAK_GUB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27009">
        <property type="entry name" value="RUST RESISTANCE KINASE LR10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13947">
        <property type="entry name" value="GUB_WAK_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041220519" description="Protein kinase domain-containing protein" evidence="14">
        <location>
            <begin position="28"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="304"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="363"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="391"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9679597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="682" mass="77141" checksum="750B3C20931BE198" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DEV6</accession>
    <name>A0AA39DEV6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Copper ion binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_020257</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9681294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DEV6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039145">
        <property type="entry name" value="Ribosomal_mL40_metazoa/plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13359">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L40, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13359:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9681294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="244" mass="27377" checksum="DD5A4C5E27F0DFEC" modified="2024-01-24" version="1">MIRISGIAKSKSCLAHQFIQKCFVSRTAKGKGKMKDGQPLKRSKITKKKGPVDAAAADRGPRNEAKVKMEQLENACLTAPTPIRYLTPKQKKREEEREKMGLTSNARRVELEMLKKNKSKKSELDSPMIIGTPGLDLISLGLVDAESIPKYELTVEDGRRLAKEYSRVLMRRHRARQAAESTILRLKKEAIEALPENLKAAALVPDLTPFPANRFMATLTPPIEGYIEKVMEAAKRSITKEKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DG90</accession>
    <name>A0AA39DG90_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_018740</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DG90"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001023">
        <property type="entry name" value="Os07g0541700 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052059">
        <property type="entry name" value="CR_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47973">
        <property type="entry name" value="CYSTEINE-RICH RECEPTOR-LIKE PROTEIN KINASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9682869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="85" mass="9877" checksum="31503C9F55B60B66" modified="2024-01-24" version="1">MYMFVDKSKSALLDWPKRYNIIIGVARGLLYLHEDSQLRIIHRDIKASNILLDELMNAKISDFGLAKLINDEQTHHRTQRIVGTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DGA7</accession>
    <name>A0AA39DGA7_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_018775</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DGA7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9682916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="157" mass="16901" checksum="3AA2AFD365993FDA" modified="2024-01-24" version="1">MGWPPKSLRLRDRPRGDNKRTGDPADGPISSAATFSLPLEPALIDPGPDPLKEGPCKLSSLAFFKPSGSTARPEEDGPTERRSTGVGSSTRLETGPLSSPCPQHPPALASHQLFVTAPRAQLLPFLPRADRRVPHHHPEPLLWTSQLAADFLPCPIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DGF3</accession>
    <name>A0AA39DGF3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_018616</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DGF3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.430:FF:000003">
        <property type="entry name" value="Probable disease resistance protein At5g66910"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057135">
        <property type="entry name" value="At4g27190-like_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050905">
        <property type="entry name" value="Plant_NBS-LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463:SF204">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23247">
        <property type="entry name" value="LRR_RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="167"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="415"/>
            <end position="551"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9682720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1167" mass="133013" checksum="2AC5B13F899E15F7" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DGY0</accession>
    <name>A0AA39DGY0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_017928</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9681787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DGY0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051222">
        <property type="entry name" value="PPR/CCM1_RNA-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47942:SF16">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT DOMAIN CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47942">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT (TPR)-LIKE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12854">
        <property type="entry name" value="PPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13812">
        <property type="entry name" value="PPR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="219"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="254"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="289"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="324"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="359"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="428"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="463"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9681787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="565" mass="63150" checksum="5292F0B311683A8B" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DIB2</accession>
    <name>A0AA39DIB2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">(-)-alpha-terpineol synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_017330</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DIB2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010333">
        <property type="term" value="F:terpene synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016102">
        <property type="term" value="P:diterpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00684">
        <property type="entry name" value="Terpene_cyclase_plant_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.600.10:FF:000007">
        <property type="entry name" value="Isoprene synthase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.130:FF:000001">
        <property type="entry name" value="Isoprene synthase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.130">
        <property type="entry name" value="Terpene synthase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034741">
        <property type="entry name" value="Terpene_cyclase-like_1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044814">
        <property type="entry name" value="Terpene_cyclase_plant_C1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001906">
        <property type="entry name" value="Terpene_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036965">
        <property type="entry name" value="Terpene_synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050148">
        <property type="entry name" value="Terpene_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005630">
        <property type="entry name" value="Terpene_synthase_metal-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225">
        <property type="entry name" value="OS04G0344100 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225:SF9">
        <property type="entry name" value="TERPENE SYNTHASE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01397">
        <property type="entry name" value="Terpene_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03936">
        <property type="entry name" value="Terpene_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01019">
        <property type="entry name" value="Terpene_Cyclase_Like_1_C_Termi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01604">
        <property type="entry name" value="Terpene_Cyclase_Like_1_C_Termi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01014">
        <property type="entry name" value="Terpene_Cyclase_Like_1_N-term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Terpene synthase N-terminal" evidence="5">
        <location>
            <begin position="58"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="Terpene synthase metal-binding" evidence="6">
        <location>
            <begin position="292"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9685256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="590" mass="68943" checksum="796FE392914681B3" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DJM3</accession>
    <name>A0AA39DJM3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Lachrymatory-factor synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_016788</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DJM3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07821">
        <property type="entry name" value="PYR_PYL_RCAR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053249">
        <property type="entry name" value="LFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019587">
        <property type="entry name" value="Polyketide_cyclase/dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33789:SF5">
        <property type="entry name" value="BET V I_MAJOR LATEX PROTEIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33789">
        <property type="entry name" value="LACHRYMATORY-FACTOR SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10604">
        <property type="entry name" value="Polyketide_cyc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9684487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="188" mass="20683" checksum="4409B42F2DE0431C" modified="2024-01-24" version="1">MSSILCKLILRVSKLSRGPPHSLGISLAQALAVGNGKWRGTVGSIVDAPIDKVWAMVSQTKRLPEWMPMVQRCSDLDGDEGVPGYIRLVSGFMFPQQDGERSWVKEKLVFMNPSSHSYAYKMEASNVGLDGSVNSLKLVDYGDDSTLVDWSFEINPLEGACEDSIIDYLGFLYKSCINRIKGAIEASD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DK38</accession>
    <name>A0AA39DK38_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Cellulose synthase-like protein H1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_015394</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DK38"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016760">
        <property type="term" value="F:cellulose synthase (UDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030244">
        <property type="term" value="P:cellulose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000145">
        <property type="entry name" value="Cellulose synthase-like protein H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005150">
        <property type="entry name" value="Cellulose_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13301">
        <property type="entry name" value="X-BOX TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03552">
        <property type="entry name" value="Cellulose_synt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="587"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="641"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="676"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="700"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="136"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="464"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="271"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR605150-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR605150-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR605150-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9686470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="725" mass="81770" checksum="3145CD7114ABC7F0" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DK63</accession>
    <name>A0AA39DK63_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">glucan endo-1,3-beta-D-glucosidase</fullName>
            <ecNumber evidence="4">3.2.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PVL29_015435</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;3)-beta-D-glucosidic linkages in (1-&gt;3)-beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.39"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the glycosyl hydrolase 17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.39" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DK63"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009506">
        <property type="term" value="C:plasmodesma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000002">
        <property type="entry name" value="Glucan endo-1,3-beta-glucosidase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1040:FF:000001">
        <property type="entry name" value="Glucan endo-1,3-beta-glucosidase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000490">
        <property type="entry name" value="Glyco_hydro_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044965">
        <property type="entry name" value="Glyco_hydro_17_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012946">
        <property type="entry name" value="X8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32227">
        <property type="entry name" value="GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00332">
        <property type="entry name" value="Glyco_hydro_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07983">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00768">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00587">
        <property type="entry name" value="GLYCOSYL_HYDROL_F17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14 16" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0611">Plant defense</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 17" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041212125" description="glucan endo-1,3-beta-D-glucosidase" evidence="17">
        <location>
            <begin position="22"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="X8" evidence="18">
        <location>
            <begin position="382"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU004336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ9686545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="471" mass="51192" checksum="06E6F48EC7E2BDB2" modified="2024-01-24" version="1">MERRAFTFFLLVISIFAFADAGSIGVNYGRIANNLPSAVKVVQLLKSHGIERVKVFDTDPAVLKALGGSGIKVTVDLPNELLISAAKRQSFANAWVQKNVADYYPATKIEAIAVGNEVFVDPHNTTLSLVPALKNIHEALVKYNLHSHIKVSSPIALSALQSSYPSSAGSFRQELIEPVFKPMLEFLRQTGSYLMVNAYPFFAYEANSDVISLDYALFRENPGVVDAGNGLRYFNLFDAQIDAVFAAMSTLKYNDIKMVVTETGWPSKGDENEIGASVANAAAYNGNLVRRILTGGGTPLRPKADLDVFLFALFNENKKPGPSSERNYGLFYPNEEKVYDIPFTVEGLKNYRDVQSPVTGGVRVSTPVTRGGSVSPSSSGQTWCVANGEIGKEKLQAALDYACGEGQADCHPIQPGATCYDPNTLVAHASFAFNSYYQKKGRVIGTCDFQGAAYVVTQAPRFGKCEFPTGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DL85</accession>
    <name>A0AA39DL85_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Myb/SANT-like DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_015162</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DL85"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000104">
        <property type="entry name" value="trihelix transcription factor ASIL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044823">
        <property type="entry name" value="ASIL1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044822">
        <property type="entry name" value="Myb_DNA-bind_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31307:SF16">
        <property type="entry name" value="OS05G0560600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31307">
        <property type="entry name" value="TRIHELIX TRANSCRIPTION FACTOR ASIL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13837">
        <property type="entry name" value="Myb_DNA-bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Myb/SANT-like DNA-binding" evidence="7">
        <location>
            <begin position="26"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9686137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="304" mass="34244" checksum="C6A64F9F6CEA46D8" modified="2024-01-24" version="1">MNDGTGTPSPATAPPRQLQPLACREDCWTEDATHTLIEAWGDRYLELNRGNLRQKHWQEVADAVNALHGHLKKARRTDVQCKNRIDTLKKKYKIEKSRVSDSNGALTSQWPFYERLDALIGSNMPAKKPSPPVYRKTPPMLPPVPVAPRSVMHKRPAPVTADESFRRNFSVVAAAAAAVEEVEEAESARSESDGDGGREGVKELAQAIVRFGEIYEKVEESKQKQMIDLEVKRMQFARDLEIQRMKLFMDTQVQLEKIKQAKRSSGNELRIMKGLLIGGVGDEKKDHLIHLDKVSDQMEDGFSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DM83</accession>
    <name>A0AA39DM83_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">serine O-acetyltransferase</fullName>
            <ecNumber evidence="3">2.3.1.30</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_012555</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.30" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9689948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DM83"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009001">
        <property type="term" value="F:serine O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03354">
        <property type="entry name" value="LbH_SAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.10.10:FF:000002">
        <property type="entry name" value="Serine acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3130.10">
        <property type="entry name" value="serine acetyltransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018357">
        <property type="entry name" value="Hexapep_transf_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045304">
        <property type="entry name" value="LbH_SAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010493">
        <property type="entry name" value="Ser_AcTrfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042122">
        <property type="entry name" value="Ser_AcTrfase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005881">
        <property type="entry name" value="Ser_O-AcTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053376">
        <property type="entry name" value="Serine_acetyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01172">
        <property type="entry name" value="cysE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041874">
        <property type="entry name" value="EPS_EpsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42811">
        <property type="entry name" value="SERINE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06426">
        <property type="entry name" value="SATase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00971">
        <property type="entry name" value="SATase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00101">
        <property type="entry name" value="HEXAPEP_TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Serine acetyltransferase N-terminal" evidence="7">
        <location>
            <begin position="68"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9689948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="307" mass="33234" checksum="CA6298DA2236DEF0" modified="2024-01-24" version="1">MAACIHDSRTSTEAAACPFSLDSADHQCRIRKSPCRFAKKCKPSFQNLVSCDPIKWVRLQADKEDDVLWLKIREQALCDMEQEPILSKYYCSSILLHDSLESALSNQLAAKLSNTDLPRSALMEVFARVLAEDEEVRRAIRADLKAVKERDPACISYAHCFLNFKGFLACQAHRVAHKLWSQGRIVMALLIQSRVSEVFAVDIHPGANLGHGVVLDHATGIVIGETAVVGDNVTILHNVTLGGTGKVNGDRHPKVGDGVLIGAGTKVLGSIRVGDRAKIGAGSVVLKEVPPETTSVGNPARLVGLKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DML7</accession>
    <name>A0AA39DML7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Receptor-like serine/threonine-protein kinase</fullName>
            <ecNumber evidence="16">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">PVL29_012687</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="25">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="25">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="16"/>
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    <keyword evidence="7 16 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="5 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 19" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
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        <location>
            <begin position="19"/>
            <end position="824"/>
        </location>
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        <location>
            <begin position="431"/>
            <end position="455"/>
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        <location>
            <begin position="18"/>
            <end position="138"/>
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            <begin position="274"/>
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        <location>
            <begin position="332"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="511"/>
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        </location>
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        <location>
            <position position="539"/>
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        <ligand>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
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        </source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAJ9690161.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="824" mass="91825" checksum="3E3F67B9247F7C22" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DMX2</accession>
    <name>A0AA39DMX2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Chromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_013000</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAJ9690623.1"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="entry name" value="Chromo_domain"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
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    </dbReference>
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        <property type="entry name" value="CHROMO_2"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="483"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="318"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="456"/>
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        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
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            <end position="87"/>
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    </feature>
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        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="318"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="353"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="460"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9690623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="546" mass="62501" checksum="7C788D95FF4BFB5A" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DN11</accession>
    <name>A0AA39DN11_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_013080</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DN11"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000344">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000377">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.430">
        <property type="entry name" value="Galactose-binding lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013857">
        <property type="entry name" value="NADH-UbQ_OxRdtase-assoc_prot30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15020">
        <property type="entry name" value="FLAVIN REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15020:SF50">
        <property type="entry name" value="UPF0659 PROTEIN YMR090W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08547">
        <property type="entry name" value="CIA30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13460">
        <property type="entry name" value="NAD_binding_10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-binding" evidence="3">
        <location>
            <begin position="138"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="NADH:ubiquinone oxidoreductase intermediate-associated protein 30" evidence="2">
        <location>
            <begin position="275"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="NAD(P)-binding" evidence="3">
        <location>
            <begin position="450"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="11"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9690743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="605" mass="66472" checksum="D042B03CE7EB96A1" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DNB7</accession>
    <name>A0AA39DNB7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_014142</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071561">
        <property type="term" value="C:nucleus-vacuole junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034271">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034272">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045324">
        <property type="term" value="P:late endosome to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13980">
        <property type="entry name" value="STKc_Vps15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:003562">
        <property type="entry name" value="Os02g0796700 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001722">
        <property type="entry name" value="Phosphoinositide 3-kinase regulatory subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000370">
        <property type="entry name" value="phosphoinositide 3-kinase regulatory subunit 4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000722">
        <property type="entry name" value="Protein kinase family protein / WD-40 repeat family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000209">
        <property type="entry name" value="Protein kinase family protein / WD-40 repeat family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055231">
        <property type="entry name" value="2AA_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045162">
        <property type="entry name" value="Vps15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17583">
        <property type="entry name" value="PHOSPHOINOSITIDE 3-KINASE REGULATORY SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17583:SF0">
        <property type="entry name" value="PHOSPHOINOSITIDE 3-KINASE REGULATORY SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22956">
        <property type="entry name" value="VPS15-like_hel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 10" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="27"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="9">
        <location>
            <begin position="535"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="1126"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="1512"/>
            <end position="1545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="413"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="422"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9688297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1545" mass="172271" checksum="454E1CD5CF02D804" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DNW2</accession>
    <name>A0AA39DNW2_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_013709</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DNW2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42861">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9691601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="69" mass="7772" checksum="6869A501E51FBA68" modified="2024-01-24" version="1">MHRVSKGAPEQILNLAHNKSEIERRVHAVIDKFADRGLQSLAVAYQEVYGNLLATCLSSTHLDMIVQRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DQ68</accession>
    <name>A0AA39DQ68_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">MADS-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_013772</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DQ68"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00265">
        <property type="entry name" value="MADS_MEF2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1810.10:FF:000006">
        <property type="entry name" value="Agamous-like MADS-box protein AGL62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033896">
        <property type="entry name" value="MEF2-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11945">
        <property type="entry name" value="MADS BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11945:SF775">
        <property type="entry name" value="MADS-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00404">
        <property type="entry name" value="MADSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="6">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9691690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="197" mass="21865" checksum="135B2CD846EF37BD" modified="2024-01-24" version="1">MGRKKIEIRKIEKKSSLKVTFSKRRTGLFKKANELCVLCSAEATVIVFSPGGRAFVFGHPTADAVIDRFLGRDTDTSSRVVVPAEQVVHGQVQRQYLEAVGRAEVREEGGFWWDAPIENMGLNELEQSREMTLMMMMVMESESGAGPSSTTMVEYAAPPQEYNSSAVLHARDLAAHSHSQTAPQGFDFGFGFDEPRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DQH5</accession>
    <name>A0AA39DQH5_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_012188</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9693323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DQH5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000129">
        <property type="entry name" value="Leucine-rich repeat receptor-like kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000041">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000111">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001347">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001646">
        <property type="entry name" value="Putative inactive leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046956">
        <property type="entry name" value="RLP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063">
        <property type="entry name" value="LRR RECEPTOR-LIKE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063:SF16">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041459588" evidence="13">
        <location>
            <begin position="23"/>
            <end position="1125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1069"/>
            <end position="1091"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="14">
        <location>
            <begin position="35"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="15">
        <location>
            <begin position="103"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="15">
        <location>
            <begin position="399"/>
            <end position="603"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9693323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1125" mass="125093" checksum="1E1C8408964BD258" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DS53</accession>
    <name>A0AA39DS53_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_011343</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DS53"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.430:FF:000003">
        <property type="entry name" value="Probable disease resistance protein At5g66910"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057135">
        <property type="entry name" value="At4g27190-like_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050905">
        <property type="entry name" value="Plant_NBS-LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463:SF220">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23247">
        <property type="entry name" value="LRR_RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="169"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9692227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="884" mass="101865" checksum="AA975D19C15C0CBD" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DSG8</accession>
    <name>A0AA39DSG8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">FAS1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_010119</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">May be a cell surface adhesion protein.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the fasciclin-like AGP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DSG8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009834">
        <property type="term" value="P:plant-type secondary cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.180.10">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036378">
        <property type="entry name" value="FAS1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000782">
        <property type="entry name" value="FAS1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045003">
        <property type="entry name" value="FLA_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32077">
        <property type="entry name" value="FASCICLIN-LIKE ARABINOGALACTAN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32077:SF65">
        <property type="entry name" value="FASCICLIN-LIKE ARABINOGALACTAN PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02469">
        <property type="entry name" value="Fasciclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00554">
        <property type="entry name" value="FAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82153">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="4" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041468552" description="FAS1 domain-containing protein" evidence="9">
        <location>
            <begin position="25"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="10">
        <location>
            <begin position="87"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="27"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="27"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="40"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9694479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="253" mass="26251" checksum="1ED5C57B5781CD0E" modified="2024-01-24" version="1">MMKQLLSPTLLLLIFFLLCATTSGQSSAPATAPSGPTTVSGQSSPPAPATSGPSGPPDIISVLQKASNSQVDVLINTQLKKSNQGFTVFAPNDSAFSDLKTGTLNSFTDQQKAALTKYHAGENTVEFPLNVVGNGTHANMTTGLVNTTVDSTVYSDGQLAVYEIPQVLLSQGILSPQAPAPAPLAPKPKKAAPLNALAPTRSTTVSVDSSDATGLPHYAPTVVSIGVAVLAQCPYPYELSVRLMDDDVLIFRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DX45</accession>
    <name>A0AA39DX45_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Potassium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_007670</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Potassium transporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the HAK/KUP transporter (TC 2.A.72.3) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DX45"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015079">
        <property type="term" value="F:potassium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003855">
        <property type="entry name" value="K+_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053952">
        <property type="entry name" value="K_trans_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053951">
        <property type="entry name" value="K_trans_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00794">
        <property type="entry name" value="kup"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30540">
        <property type="entry name" value="OSMOTIC STRESS POTASSIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30540:SF14">
        <property type="entry name" value="POTASSIUM TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02705">
        <property type="entry name" value="K_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22776">
        <property type="entry name" value="K_trans_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0630">Potassium</keyword>
    <keyword evidence="4 10" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="77"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="168"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="209"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="232"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="282"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="313"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="353"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="401"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="435"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="465"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="490"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="529"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="domain" description="K+ potassium transporter integral membrane" evidence="11">
        <location>
            <begin position="40"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="domain" description="K+ potassium transporter C-terminal" evidence="12">
        <location>
            <begin position="542"/>
            <end position="770"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU321113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF22776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9698725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="772" mass="85863" checksum="90F909C650F5FE89" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DYJ7</accession>
    <name>A0AA39DYJ7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_004522</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. ADCK protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DYJ7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05121">
        <property type="entry name" value="ABC1_ADCK3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004147">
        <property type="entry name" value="ABC1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050154">
        <property type="entry name" value="UbiB_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10566">
        <property type="entry name" value="CHAPERONE-ACTIVITY OF BC1 COMPLEX CABC1 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10566:SF124">
        <property type="entry name" value="PROTEIN KINASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03109">
        <property type="entry name" value="ABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="223"/>
            <end position="570"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9702821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="758" mass="85643" checksum="057349F392333F88" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DYM9</accession>
    <name>A0AA39DYM9_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_004614</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DYM9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9702941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="122" mass="13814" checksum="97E63CE2E7A65448" modified="2024-01-24" version="1">MTVVLTIIVYSCSQAHQNLRRSPGGPNPIHLDIEDPMKSEAYGIYEESPKGSNAIHSKMEDQIGKSTYKPYKETLDGPNPIHNKMKGQGRMNTYEIYRTTPKGPNLIHNNEGIFENNAYDIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E2R8</accession>
    <name>A0AA39E2R8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_004639</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E2R8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005162">
        <property type="entry name" value="Retrotrans_gag_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33223">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33223:SF8">
        <property type="entry name" value="OS04G0172440 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03732">
        <property type="entry name" value="Retrotrans_gag"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Retrotransposon gag" evidence="2">
        <location>
            <begin position="193"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="302"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="356"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="314"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9702967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="481" mass="53689" checksum="30A92121710A0D2B" modified="2024-01-24" version="1">MATLHGSTPSLLRRAEGCVLPKSSDSSVEMSDELPSTLASIQEFMAGVSRRLDQIESSSQDPHPVGIVTDETIPHASQIAQALPPRASPSIPFHLADHYETIPLPTITVPPPIVATTDDTRLAEQEARVERLESRMRQIKLQDGGLTWDDRDGIPTASLPAKFHMPNMESYTGIGCLKIHLRLYSTVMRAHGIDGAHLVAFSPFEPSRLRTWEDIAHAFLTQFASSADIDVSRRELEATKQRPNESISSFVSRWRAKDQIDMVLRNLRPRFARRLMGVPFQDLKSLVHAAFSVEEAIARGLWTDTTPSPDSKGKKPYQRPAHHPSYRPLTVRAHFSHPQYQYQLVYVQEPYIAQTTMQPQPPHPRATTHPLPKPYAQRPARQFTLLGMTLTRAFEKLRDAGLIVPLAPCPLPHPIPPHFRSHEHCLYHQIQGHDTERCSALHHAIQDLIDSGLVNLAGPSVTTNPLPVHSTHAVPPPPSLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E434</accession>
    <name>A0AA39E434_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Eukaryotic translation initiation factor 4B2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_000677</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E434"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010433">
        <property type="entry name" value="EIF-4B_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32091">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32091:SF20">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06273">
        <property type="entry name" value="eIF-4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="451"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="231"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="280"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="307"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="348"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="470"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="508"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9708776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="523" mass="57739" checksum="86611BFB30C8B2E9" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E5I4</accession>
    <name>A0AA39E5I4_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_002377</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E5I4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9707350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="321" mass="35774" checksum="D0737C7D00CC04DF" modified="2024-01-24" version="1">MNSIPRLSRILQYSPLYDLVFGTRHDTILSVLGSLLLGQIRFPVSIVLSGTRLDTIPFVLDSPILPIFDSPSVSYSPLLIEYDLFSGTHKDTILSILDSRLLGRIRFPVSIVFSDTRPDTILSVLDSPKLGQIRFPVCLVFSSTQSDTISSPVLARYYSFGLRFAGTRTNSIPCLYRILRYSPGYDPFYLGFSDTRTNSIPRLYRILQYSAGYDLFSSTRQDTILSVLDSPVLGRIGFPVSIVFSSTRKDTILSILDSLVLGQIRFPVSIVFSGNRPDTILSVLDSSILGKFDSLSISYSPVLGRIRSYLPYSLGYHSFGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E824</accession>
    <name>A0AA39E824_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_000829</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E824"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF78">
        <property type="entry name" value="OS12G0587133 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9709030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="613" mass="70226" checksum="8844C567F080D591" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YGA8</accession>
    <name>A0AA38YGA8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ycf20-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_026466</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ycf20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9669912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YGA8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007572">
        <property type="entry name" value="Uncharacterised_Ycf20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33787">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33787:SF4">
        <property type="entry name" value="YCF20-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04483">
        <property type="entry name" value="DUF565"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="128"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="153"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9669912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="209" mass="22420" checksum="24A7A664D655E475" modified="2024-01-24" version="1">MACRMGGIMLQSTLSVPESESSDCSYITFTTCCTIQNWNNEPSISGQISKLGMKSAPFPRKSFQTRRHGWKTAFALDTGGIPDNGGQENLNGNGPDLGQTRLGRIVSAGGRQLLAKLNSARKNFPMKVFLLLLGFYTANALATILGQTGDWDVLVAGVVVAVIEGIGMLVYKKSSSQSTGRLQSLVVMINYWKAGVCLGLFVDAFKLGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YHP4</accession>
    <name>A0AA38YHP4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Glycosyltransferases</fullName>
            <ecNumber evidence="12">2.4.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_026910</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Involved in the synthesis of glucuronoxylan hemicellulose in secondary cell walls.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Golgi apparatus membrane</location>
            <topology evidence="1 12">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the glycosyltransferase 43 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.-.-" evidence="12"/>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YHP4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015018">
        <property type="term" value="F:galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042285">
        <property type="term" value="F:xylosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010417">
        <property type="term" value="P:glucuronoxylan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009834">
        <property type="term" value="P:plant-type secondary cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000096">
        <property type="entry name" value="Glycosyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005027">
        <property type="entry name" value="Glyco_trans_43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10896:SF17">
        <property type="entry name" value="BETA-1,4-XYLOSYLTRANSFERASE IRX14H-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10896">
        <property type="entry name" value="GALACTOSYLGALACTOSYLXYLOSYLPROTEIN 3-BETA-GLUCURONOSYLTRANSFERASE BETA-1,3-GLUCURONYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03360">
        <property type="entry name" value="Glyco_transf_43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 12" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="451"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="477"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9670648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="513" mass="57307" checksum="733849C2D97484CC" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YIL9</accession>
    <name>A0AA38YIL9_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_027214</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9671114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YIL9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9671114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="204" mass="22004" checksum="5384BF4C5C2A242E" modified="2024-01-24" version="1">MHVPLVASTSTAGDDAIEVIEACISETSETMLRLALSLVWDVGALRCRHGGSEGNSSVPCPSAEHHAEDQPMHPTLVRQPSAKEIELEDSLVPTPYSPIGMGPSPVPTYSEPLVPQSQAEASFPAEVTIEEPQECEGAGGSGSQALKPSSMKRYQRTAKRIVKRPPACKSPFVAQCVKQFPKIPHIDRVVADYALTEMVDPRYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YJT4</accession>
    <name>A0AA38YJT4_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_025437</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YJT4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9671729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="99" mass="10772" checksum="16DD513739F5F3A4" modified="2024-01-24" version="1">MGEYPYNCWNMPASEASPSVGGIGVGVGDGYSCNWRCNNKAREGKDSRLRLQCVKGKEGAILGKTTSFQCLTHQNDVILRKFTNHSVHENPADSANSPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YKE1</accession>
    <name>A0AA38YKE1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_025607</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YKE1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050231">
        <property type="entry name" value="Iron_ascorbate_oxido_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47990">
        <property type="entry name" value="2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0408">Iron</keyword>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="4">
        <location>
            <begin position="176"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9672035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="322" mass="36607" checksum="A5F702FA7EDA088B" modified="2024-01-24" version="1">MDEIVEPPFKEFCKHLSGNCVDEVESGTAFQKFFAVDELDLPLVGLNLVNLGPLESEECKRQICDAASNWGFLQLVNQGISLDIVSRLRSEQVKLFRQPFNKKANEKLLNLPSGSYRWEHKLQALSCNFHGLKPFTYPLSTIDEFAVKVFEMGQQIAQILAENLSGKSTFCSENCLPSSCFLRMNRYPPCLVSSNVFGIVPHIDSDFLTILHQDEVGRLQIIKDGKWIGVKLNPEALIINIGNLFQAWSNGIYNSVEHLVLNHQKVERFSIAYFFCSLADAVIQSYREPSIYRSFSFREYQKQVQEDAKSTGNKVGLSRSYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YL03</accession>
    <name>A0AA38YL03_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_025853</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YL03"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="132"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="204"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="158"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9672394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="244" mass="23066" checksum="B8168286D1EA7F9D" modified="2024-01-24" version="1">MTPVRYTGSEPESSLTGPSAVSGDKLAGVEIGGLLDGNIPGDSAGVAELEGMLPGASGVVAVAGTSAGDSAVGVDASGGVAEGVDASGDVAGVAAGVADGDADGGVVDSGDGTGEDTCVVGLTAGVALGGVTGEAVGGEEDGDSDGDWTGLAAGGDAGDLTGDADSGDLAGDADSGDLAGDANSGDLAGGFNAGDGEVGDLGDGAGALAPCTTPAPDTHKTNRATTTRRNIFVWKKFRLQKRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YMX7</accession>
    <name>A0AA38YMX7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DYW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_023157</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. PCMP-H subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YMX7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000475">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At5g40410, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000407">
        <property type="entry name" value="Putative pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032867">
        <property type="entry name" value="DYW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046849">
        <property type="entry name" value="E2_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF533">
        <property type="entry name" value="DYW DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14432">
        <property type="entry name" value="DYW_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20430">
        <property type="entry name" value="Eplus_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="229"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="330"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="DYW" evidence="4">
        <location>
            <begin position="535"/>
            <end position="627"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9673425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="627" mass="70371" checksum="DDCA0D539EA8B65A" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YRU7</accession>
    <name>A0AA38YRU7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SHSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_024398</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YRU7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031107">
        <property type="entry name" value="Small_HSP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11527">
        <property type="entry name" value="HEAT-SHOCK PROTEIN 20 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="4">
        <location>
            <begin position="44"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9675454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="134" mass="15237" checksum="F9503EE212652967" modified="2024-01-24" version="1">MSSLGVWRGGEYNDWTPQSTSNVWDPFDFGFGVEKSWGPGLDDDVSALAHAHINWRETNNAHVFHTDLPGVRREELKVQVEDNNILKISGEKTRKKRKQRGSFFRRFRLPENAITDRISSALKDGVLTITVPKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YSI5</accession>
    <name>A0AA38YSI5_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_024543</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YSI5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34222:SF40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34222">
        <property type="entry name" value="GAG_PRE-INTEGRS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9675674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="122" mass="14017" checksum="6055C4C5D5033D49" modified="2024-01-24" version="1">MEDNRIFKFLVGLNVEFDEVRGRIIGRQPLPSIGEVFSEVRREESRRNVMLGKKGPRVAIEGLALVTTDGRYNKAAAFQRKSDERLRVWCDFCNKPCHTRDNCWKIHGKLANWKGKTGDKPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YUB2</accession>
    <name>A0AA38YUB2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">EamA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_022026</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the drug/metabolite transporter (DMT) superfamily. Plant drug/metabolite exporter (P-DME) (TC 2.A.7.4) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9676821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YUB2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051258">
        <property type="entry name" value="Diverse_Substrate_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42920:SF10">
        <property type="entry name" value="EAMA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42920">
        <property type="entry name" value="OS03G0707200 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="110"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="139"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="247"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="275"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="322"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="356"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="380"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="8">
        <location>
            <begin position="109"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="8">
        <location>
            <begin position="245"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9676821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="451" mass="49412" checksum="F1A1E8D38A44B666" modified="2024-01-24" version="1">MAATYPRWKTIATTSSSDSSTLPCCFFFSTSPSPRTKRGRFATLSSLLLNPHEYHLGPAKKKKSVSIIHDECISKAKVKAKFDWKKSIQKGAKSRPLWRRVLFAPKKMKSIILLNLVTVVYASNIAIVKEVETIMDPAAFSAVRFALSAIPFLPFVFRARGDVQTRNAGLELGLWISLGYLVEALGLLTSEAGRASFFSFITVIVVPLLDSMLGAIVPARTWFGVLMSVLGVAMLECSGSPPNVGDLLNFLSAIFFGIHILRTEQLSRSTKKENFLPLLGYELCVVALLSTFWYVTGGWFDGIQDSDQASWTWAVLWDSMVAFPWIPALYTGVFSTGLCLWLEIGAMRDVSATETAIIYGLEPLWGAGFAWFLLGERWGTTGWIGAALLLGGSLTVQMFGSSSSSESIGVEDGDKEVDILVSEKQKLQTGLSASPVVIISSRKDVIDMLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YUS5</accession>
    <name>A0AA38YUS5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Partial AB-hydrolase lipase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_022191</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YUS5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000091">
        <property type="entry name" value="Gastric triacylglycerol lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006693">
        <property type="entry name" value="AB_hydrolase_lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11005">
        <property type="entry name" value="LYSOSOMAL ACID LIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04083">
        <property type="entry name" value="Abhydro_lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="134"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="Partial AB-hydrolase lipase" evidence="3">
        <location>
            <begin position="292"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9677070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="696" mass="78164" checksum="3C24758D10971767" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YUU1</accession>
    <name>A0AA38YUU1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">K Homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_022215</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PNO1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YUU1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22391">
        <property type="entry name" value="KH-I_PNO1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22392">
        <property type="entry name" value="KH-I_PNO1_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000009">
        <property type="entry name" value="RNA-binding protein PNO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055212">
        <property type="entry name" value="KH-I_PNO1_first"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055211">
        <property type="entry name" value="KH_PNO1_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12826">
        <property type="entry name" value="RIBONUCLEASE Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12826:SF13">
        <property type="entry name" value="RNA-BINDING PROTEIN PNO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22891">
        <property type="entry name" value="KH_PNO1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="6">
        <location>
            <begin position="129"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9677106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="224" mass="25249" checksum="0CCD5C0D4A2D829E" modified="2024-01-24" version="1">MQSREATTSMEVENVLSEPNPSSGTFPSKPNFEPLKAHEISEGRVQFRKVSVPPHRYTPLKKVWMEIYTPIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVVDAIALLRLDELYVESFEIKDVKTLRGEHLSRAIGRLSGKGGKTKFAIENATRTRIVIADTKIHILGSFSNIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YWV3</accession>
    <name>A0AA38YWV3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF4408 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_022925</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9678183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YWV3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008480">
        <property type="entry name" value="DUF761_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33098">
        <property type="entry name" value="COTTON FIBER (DUF761)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33098:SF75">
        <property type="entry name" value="DUF4408 DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05553">
        <property type="entry name" value="DUF761"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="66"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="181"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="196"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9678183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="269" mass="30306" checksum="31E1DC591B49B9E7" modified="2024-01-24" version="1">MLRKFTCNIISRFHMAIWAVKQVLLSVGIISTVLALNPTPKISYTFSLCYGIPSFWVWLKTWLSPPYAYIIINFILIIIIIIASSSSSSSSSSSLVEGFTALDYSTVQDVLSEVSGDEEAHVKTSMVMDDSRRKCITGETAVKLESTAVAPGQNESLEATWEAIVGEHGIPATRHLRKSETWGVPPCVDGSSSLGPREREREMRKSNTSEEARSSFRETPIPFRRKDKSLSHDELNKKIEEFIQKCRNEMRLERVESDQRLMDILNRGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YX04</accession>
    <name>A0AA38YX04_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Methyl-CpG-binding domain protein 4-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_022827</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9678057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YX04"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.30:FF:000007">
        <property type="entry name" value="Methyl-CpG-binding domain protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045138">
        <property type="entry name" value="MeCP2/MBD4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15074:SF0">
        <property type="entry name" value="METHYL-CPG-BINDING DOMAIN PROTEIN 4-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15074">
        <property type="entry name" value="METHYL-CPG-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="91"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="149"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="253"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="360"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="93"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="166"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="257"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="282"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="299"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="310"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="371"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9678057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="597" mass="69115" checksum="A71B32C50912148E" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YXH7</accession>
    <name>A0AA38YXH7_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_022989</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9678254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YXH7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9678254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="91" mass="10666" checksum="56B74C38D81757A7" modified="2024-01-24" version="1">MVKVLYDKQPERINDMDWKDLEAKVAATIRLCLIISDLKRIDVKFEDEDKALMLLNSLLVFSTYENLVTTLMWGKETLDLEEITIVLLEFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YYH6</accession>
    <name>A0AA38YYH6_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_021084</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YYH6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36008">
        <property type="entry name" value="OS09G0478400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36008:SF1">
        <property type="entry name" value="OS09G0478400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9679051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="172" mass="19839" checksum="2CA4DCD65FD955F2" modified="2024-01-24" version="1">MSGVARRVFVFQRQLNTLSHDGPTDTLKRKIAGMEKIRKRRDPKKDQFYVEVPESQKFLDTATWPMTVAAVGVAVIAKLFMMYDDSKSEEMVERKIKNAPAGQGTVRMLTREEWDAIQEVRPRTPFESKFSRHNARIRTGEPLHMEDVKDWTLDVLTDALTRVEESVKRGSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YZ22</accession>
    <name>A0AA38YZ22_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">fructokinase</fullName>
            <ecNumber evidence="9">2.7.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_021182</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">May play an important role in maintaining the flux of carbon towards starch formation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>D-fructose + ATP = D-fructose 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16125"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37721"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Glycan biosynthesis; starch biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the carbohydrate kinase PfkB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.4" evidence="9"/>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YZ22"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008865">
        <property type="term" value="F:fructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006000">
        <property type="term" value="P:fructose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01167">
        <property type="entry name" value="bac_FRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1190.20:FF:000005">
        <property type="entry name" value="Probable fructokinase-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002173">
        <property type="entry name" value="Carboh/pur_kinase_PfkB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050306">
        <property type="entry name" value="PfkB_Carbo_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002139">
        <property type="entry name" value="Ribo/fructo_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43085:SF7">
        <property type="entry name" value="FRUCTOKINASE-7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43085">
        <property type="entry name" value="HEXOKINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00990">
        <property type="entry name" value="RIBOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00583">
        <property type="entry name" value="PFKB_KINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00584">
        <property type="entry name" value="PFKB_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase PfkB" evidence="12">
        <location>
            <begin position="26"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9679172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="346" mass="37480" checksum="6CC1863B7351AB4E" modified="2024-01-24" version="1">MAKNPTSGNYEDLPLNTGGADNKSSLVVCFGEMLIDFVPTVSGVSLAEAPAFKKAPGGAPANVAVGISRLGGSSAFIGKVGDDEFGYLLADILKKNNVNNSGMRFDRSARTALAFVSLRADGEREFLFFRNPSADMLFRESELDLKLLEQAKIFHYGSISLIEEPSRSTHLAAMTIAQKAGSILSYDPNLRLKLWPSAEAARKGIMSIWDKADIIKISEDEVTFLTGGDDPNDDNVVLKKLFHPNLKLLLVTEGSEGCRYYTKKFRGRVAGIKIKAVDTTGAGDAFVSGILSNIASDINLYQDEKRLREALVFANVCGALTVRERGAIPALPTKEAVLEMLQNIPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YZ39</accession>
    <name>A0AA38YZ39_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cytochrome P450 704C1-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_021163</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YZ39"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11064">
        <property type="entry name" value="CYP86A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24296">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0349">Heme</keyword>
    <keyword evidence="6 8" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="8">
        <location>
            <position position="450"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9679152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="504" mass="58190" checksum="407E2B9DD775FBFD" modified="2024-01-24" version="1">MILILLGFYVACSFMLALFGIPSFFLMIFVAKFLFVYIGEVMSKDQRPPVAGPVFNQLVHFRKLFDYQASLAQKYITYRMILPSHSEIYTADPVNVEYILKTNFTNYGKGEYHCDIMKDLFGDGIFAVDGEKWRHQRKLASYEFSTKVLRDFSTSVFQDNAAKLVLKVSMVAAAKQMMDLQDMLMKSTLDSIFKVGFGVDLNSLSGSDVFGNQFIKAFDDSNVIIKRFLNLGLEAALKHNIKVIDSFIFELIRCKREQMKNKELDRGKEDILSRFLLESKKDPKKMNDQYLRDIILSFLIAGKDTSADTLTWFFYMLCKHPLVQEKVVLEVREATKAQINISADEFAKKITEKALDNMQYLHAALTETLRLYPAVPMDGKHSEEDDILPDGFKVKKGDGMCYMAYAMGRMRCIWGEDAGEFRPERWLENGVFQPESPFKFTAFQAGPRICLGKEFAYRQMKILAAVLVNFFKFKLVDERKEARYRTMFTLHMDQGLHLYAFPRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YZD7</accession>
    <name>A0AA38YZD7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_021219</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YZD7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09917">
        <property type="entry name" value="F-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000869">
        <property type="entry name" value="Zinc finger CCCH domain-containing protein 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001235">
        <property type="entry name" value="Zinc finger CCCH domain-containing protein 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005174">
        <property type="entry name" value="KIB1-4_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044715">
        <property type="entry name" value="WDR86-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041367">
        <property type="entry name" value="Znf-CCCH_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44489">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44489:SF1">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03478">
        <property type="entry name" value="Beta-prop_KIB1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18044">
        <property type="entry name" value="zf-CCCH_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 7" id="KW-0853">WD repeat</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="10">
        <location>
            <begin position="487"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="10">
        <location>
            <begin position="580"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="618"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="741"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="8">
        <location>
            <begin position="487"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="8">
        <location>
            <begin position="580"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="512"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="521"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9679214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="907" mass="101610" checksum="2D521A5A31B4B485" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YZH7</accession>
    <name>A0AA38YZH7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_021475</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YZH7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044646">
        <property type="entry name" value="EMB1417-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46782">
        <property type="entry name" value="OS01G0757700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46782:SF1">
        <property type="entry name" value="OS01G0757700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="122"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="158"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="241"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="244"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="263"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="279"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9679566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="296" mass="34253" checksum="A450C5F7E5A90F2D" modified="2024-01-24" version="1">MLCLRYSPATITRRLDSVEIPKDPRSIVVCGAKGPRPRYPRVWKTRQRIGTISKSAKLVDCIKGLSNVKEEVYGALDSFIAWELEFPLITVKKALKTLEDQKEWKRIIQVTKWMLSKGQGRTMGSYFTLLNALAEDGRLDEAEELWTKLFSENLESMPRVFYDKMISIYYRRDMHEKMFEIFADMEELGIRPNTSIVKMVGDVFQKLGMLDKYEKLQKKYPPPKWEYRYIKGKRVRIRAKLTGESDDPGEAESDDPGEAVNEIDDKTDMNSNEMHEEADSSVDDADEPIYEAHNSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z0J2</accession>
    <name>A0AA38Z0J2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PGG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_019424</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z0J2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026961">
        <property type="entry name" value="PGG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24177">
        <property type="entry name" value="CASKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24177:SF103">
        <property type="entry name" value="PGG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13962">
        <property type="entry name" value="PGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="173"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="214"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="250"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="PGG" evidence="2">
        <location>
            <begin position="126"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9680131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="289" mass="32819" checksum="7DCFC9B2725DA185" modified="2024-01-24" version="1">MVERILDAFPMAILDRDSDGKNIVLLAVENRQTKLYEQLVQNILFNESAFRAVDNKGNSALHLAARIGDFQPYPFAALQMQWEIKWFKYVKYSVPRDFFMNLNNEDMTPKEVFRTSHKDLVKEGTKWLTDTSNSCSLVATLVTTVAFATTATVPGGMKEGSWSPNLGRHPGFIVFAISSLIALSFSATSVIAFLSILTSRHHQKDFQSDLPKKLLLALTSLFISLAAMMFCFCAGHFFLVKDEFEHTAYLVIYAIACLPIAYFAMMQFPFYFALVLQTFKRVPQRTPFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z0K5</accession>
    <name>A0AA38Z0K5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DUF659 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_019454</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z0K5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007021">
        <property type="entry name" value="DUF659"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008906">
        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32166:SF122">
        <property type="entry name" value="OS09G0499600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32166">
        <property type="entry name" value="OSJNBA0013A04.12 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04937">
        <property type="entry name" value="DUF659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF659" evidence="2">
        <location>
            <begin position="41"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="HAT C-terminal dimerisation" evidence="3">
        <location>
            <begin position="391"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="535"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="535"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="549"/>
            <end position="576"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9680166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="603" mass="69146" checksum="F23B0A0FF99624BB" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z0M3</accession>
    <name>A0AA38Z0M3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_019484</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the peptidase M41 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z0M3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045037">
        <property type="term" value="P:protein import into chloroplast stroma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19501">
        <property type="entry name" value="RecA-like_FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000083">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000352">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FTSH 7, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.760:FF:000011">
        <property type="entry name" value="Probable inactive ATP-dependent zinc metalloprotease FTSHI 1, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.760">
        <property type="entry name" value="Peptidase M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000642">
        <property type="entry name" value="Peptidase_M41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037219">
        <property type="entry name" value="Peptidase_M41-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23076:SF111">
        <property type="entry name" value="INACTIVE ATP-DEPENDENT ZINC METALLOPROTEASE FTSHI 1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23076">
        <property type="entry name" value="METALLOPROTEASE M41 FTSH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01434">
        <property type="entry name" value="Peptidase_M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140990">
        <property type="entry name" value="FtsH protease domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="254"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="12">
        <location>
            <begin position="427"/>
            <end position="574"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9680196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="907" mass="102228" checksum="54059C1FC93C2354" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z2H2</accession>
    <name>A0AA38Z2H2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Importin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_020168</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the XPO2/CSE1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9681151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z2H2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005049">
        <property type="term" value="F:nuclear export signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006611">
        <property type="term" value="P:protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000057">
        <property type="entry name" value="Exportin-2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005043">
        <property type="entry name" value="XPO2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013713">
        <property type="entry name" value="XPO2_central"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997:SF8">
        <property type="entry name" value="EXPORTIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997">
        <property type="entry name" value="IMPORTIN-7, 8, 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03378">
        <property type="entry name" value="CAS_CSE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08506">
        <property type="entry name" value="Cse1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="8">
        <location>
            <begin position="29"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9681151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="979" mass="109729" checksum="93A0A47827D456C0" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z6N1</accession>
    <name>A0AA38Z6N1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Disease resistance N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_019114</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9683391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z6N1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14798">
        <property type="entry name" value="RX-CC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038005">
        <property type="entry name" value="RX-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="4">
        <location>
            <begin position="5"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9683391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="319" mass="35778" checksum="57039A036698EFE9" modified="2024-01-24" version="1">MAASVVTLFQWRLNSMLLQENEILLGVKDQIESLINKLDAVGSFTGDVDRILGREICHLTWASELRGMLRKAEDFVDDFIINAYGQTEKDRENSIAEFGSELQNINSRVSEILNRRPRLEASLMLEEIQRFLRSNFVFDKDKETALASCMLNYINLPYDLKLSLLFCCALPGDYRKTKDALIPVLVATGLIQEKPGELMEDKAQENIERLVDLGMLTEDTDLSLTVMNAEDFLISTANSDSIIPPTARHVSIPDAENTITNTNSLLHSLFVSAKCGLSKPSADCLETVLCNAKLLRVLDLENTQLKVFVCEAFQHNRAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z7D7</accession>
    <name>A0AA38Z7D7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_016339</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9683793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z7D7"/>
    <dbReference type="SMR" id="A0AA38Z7D7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd13276">
        <property type="entry name" value="PH_AtPH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000286">
        <property type="entry name" value="PH-protein kinase domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051707">
        <property type="entry name" value="PI-Interact_SigTrans_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14336:SF20">
        <property type="entry name" value="PLECKSTRIN-LIKE (PH) DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14336">
        <property type="entry name" value="TANDEM PH DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="1">
        <location>
            <begin position="26"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9683793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="143" mass="16671" checksum="68835AEE649B8B37" modified="2024-01-24" version="1">MWSLWRAATGTADDSDDYGGVEFWSNPERTGWLTKQGEYIKTWRRRWFVLKQGKLFWFKESTITRASRPRGVIPVASCLTVKGAEDVLNKQFAFELSTRTETMYFIADSEKEKEDWINSIGRSIVQHSRSVTDSEIVDYDSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZB01</accession>
    <name>A0AA38ZB01_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_017342</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZB01"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="75"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9685272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="142" mass="16238" checksum="EED0A547D94D9B7D" modified="2024-01-24" version="1">MVMEEKVEEEARASELESSVGLHPHGKLLSYLGLRFSIFLALLPSSSSISQLSTLHLKLLQAEQELCHLKSGRKEDSKANEIASLRAKVVELERSEAELKARVEKLTREPDKIFPHFSLLISSYFPHYFLRKIIGKILQYFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZB72</accession>
    <name>A0AA38ZB72_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_017694</name>
        <name evidence="9" type="ORF">PVL29_017731</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endomembrane system</location>
            <topology evidence="7">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZB72"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01868">
        <property type="entry name" value="Rab11_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000274">
        <property type="entry name" value="ras-related protein RABA5a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0636">Prenylation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9685752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9685791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="226" mass="25185" checksum="0A0D06EC6691B60C" modified="2024-01-24" version="1">MSNFYKGDYSQKIDYVFKVVLIGDSAVGKSQLLARFARNEFNLDSKATIGVEFQTKTLVIDHKTVKAQIWDTAGQERYRAVTSAYYRGAVGAMLVYDITKRQSFDHVARWLQELRGHADKNIVIMLVGNKSDLGTLQAVPTEDAKEFAERENLFFMETSALEATNVETAFLSVLTEIYRIISKKTLVANEEVESGGNSSLLKGTKIIVPGKEPESEGSKYNCCLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZDV9</accession>
    <name>A0AA38ZDV9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF4219 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_015894</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZDV9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35317:SF11">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35317">
        <property type="entry name" value="OS04G0629600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9687216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="93" mass="10390" checksum="7DFEEB2C1A80DA39" modified="2024-01-24" version="1">MESSLTTAPSILDGDNCETWAVKMIVHLQALDVWEAVKENYEVPPLEANLTMTQMKLHKERKTRKAKAKACLFAAVSPSIFIKIMKIDSAAEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZE74</accession>
    <name>A0AA38ZE74_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_016033</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZE74"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000022">
        <property type="entry name" value="E3 ubiquitin-protein ligase RING1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15710">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE PRAJA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15710:SF18">
        <property type="entry name" value="RING-TYPE E3 UBIQUITIN TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="186"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="146"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="146"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9687381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="261" mass="29951" checksum="34C9721C0B774781" modified="2024-01-24" version="1">MRTYHYYWCQHCQRTMRIASGILSEILLCPFCSCRLQYEFHAPRQRLVPTFTGLGVLAAELLEDMDVIMLPRRRQNTDLNGVLWGSEMRIPSINVLSRVGRHLRRPPRPGRPVAAPENVAAPRVGGRNFTMRDVLNEAMEELTHNEVTGENTQNDRPGPPPAAPSAIEALPSVRLTPTHLRNDPCCPVCKEEYQPGEEAREMPCKHMYHSDCIVPWLRIHNSCPVCRHELQASPNPHDLHNTRAENFDVEEVMNRLVWPWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZE92</accession>
    <name>A0AA38ZE92_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycosyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_015951</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="5"/>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZE92"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035251">
        <property type="term" value="F:UDP-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000060">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000080">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050481">
        <property type="entry name" value="UDP-glycosyltransf_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48048">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48048:SF45">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9687274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="475" mass="52917" checksum="05D29716744F675F" modified="2024-01-24" version="1">MEQTEMVFIPFPVIGHLASALEIAKLITKRDPRFSITIFIMKFPFGSTDAMDTDSDSIRFVTLPPVEVSSETTPSGHFFSEFLKVHIPLVRDAVHELTRSNSVRLSGFVIDMFCTHMIDVADEFGVPSYLFFSSGAAVLGFLLHVQFLHDYEGLDINEFKDSDAELDVPTFVNSVPGKVFPAWMFDKESGGAEMLLYHTRRFREVKGILVNTFIELESHAIQSLSGSTVPEVYPVGPILNTRMGSGGAQQDASAIMSWLDDQPPSSVIFLCFGSMGSFGADQIKEIAYGLEHSGHRFLWSLRQPPQKGKMEFPGGYENIEEVLPEGFLHRTARIGKVIGWAPQIAVLAHSAVGGFVSHCGWNSLLESIWYGVPVATWPIYAEQQINAFQMVKGLGLAVEIKIDYNEDSDYVVSAHEIENGLRNLMNINSEVRKKRKEMEKISRKVMIDGGSSHFSLGHFIEDMMDSKVMKGKDAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZF29</accession>
    <name>A0AA38ZF29_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein TIFY</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Jasmonate ZIM domain-containing protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_013897</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Repressor of jasmonate responses.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="2">The jas domain is required for interaction with COI1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the TIFY/JAZ family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9687891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZF29"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031347">
        <property type="term" value="P:regulation of defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000022">
        <property type="term" value="P:regulation of jasmonic acid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009611">
        <property type="term" value="P:response to wounding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018467">
        <property type="entry name" value="CCT_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040390">
        <property type="entry name" value="TIFY/JAZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010399">
        <property type="entry name" value="Tify_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33077:SF140">
        <property type="entry name" value="PROTEIN TIFY 10B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33077">
        <property type="entry name" value="PROTEIN TIFY 4A-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09425">
        <property type="entry name" value="Jas_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06200">
        <property type="entry name" value="tify"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00979">
        <property type="entry name" value="TIFY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51320">
        <property type="entry name" value="TIFY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1184">Jasmonic acid signaling pathway</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tify" evidence="4">
        <location>
            <begin position="131"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="238"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU369065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9687891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="268" mass="28571" checksum="9CFA462F9F5BB9CE" modified="2024-01-24" version="1">MSSSSDFADSGRFTGQRAPARGPEKSSFSQTCSLLSQYIKEKGTFGDLSLGMTCSLEGNGTPESLRQTATTTTMNLFPMTERSAGISGIPARNMNLKSMNLFPQQAGFGSSVSKDDAPKIVNSSVNKSGNVEPQTAQMTIFYGGQVIVFNDFPADKAKEVMRLAGMGSSPVPSTPVKNPIDAGGMAPSTSNVVPNFANSLIQERIQRPAQPVACELPIARKASLHRFLEKRKDRITARAPYNISNSPAGPHKPAESKSWLGLAAQSPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZFG2</accession>
    <name>A0AA38ZFG2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Sucrose synthase</fullName>
            <ecNumber evidence="3 7">2.4.1.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_013876</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">Sucrose-cleaving enzyme that provides UDP-glucose and fructose for various metabolic pathways.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>an NDP-alpha-D-glucose + D-fructose = a ribonucleoside 5'-diphosphate + sucrose + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16241"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17992"/>
            <dbReference type="ChEBI" id="CHEBI:37721"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:76533"/>
            <dbReference type="EC" id="2.4.1.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the glycosyltransferase 1 family. Plant sucrose synthase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.13" evidence="3 7"/>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9687860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZFG2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016157">
        <property type="term" value="F:sucrose synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005985">
        <property type="term" value="P:sucrose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1230:FF:000001">
        <property type="entry name" value="Sucrose synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.330:FF:000001">
        <property type="entry name" value="Sucrose synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000004">
        <property type="entry name" value="Sucrose synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000006">
        <property type="entry name" value="Sucrose synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000368">
        <property type="entry name" value="Sucrose_synth_GT-B1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012820">
        <property type="entry name" value="Sucrose_synthase_pln/cyn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056736">
        <property type="entry name" value="SUS_EPBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056735">
        <property type="entry name" value="SUS_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02470">
        <property type="entry name" value="sucr_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45839">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45839:SF7">
        <property type="entry name" value="SUCROSE SYNTHASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00862">
        <property type="entry name" value="GT-B_Sucrose_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24862">
        <property type="entry name" value="SUS_EPBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24861">
        <property type="entry name" value="SUS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sucrose synthase N-terminal" evidence="10">
        <location>
            <begin position="9"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Sucrose synthase EPBD" evidence="11">
        <location>
            <begin position="155"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="Sucrose synthase first GT-B" evidence="9">
        <location>
            <begin position="265"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="8">
        <location>
            <begin position="566"/>
            <end position="728"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU280817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF24861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF24862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9687860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="806" mass="92751" checksum="E32C0B0E1C553583" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZFM8</accession>
    <name>A0AA38ZFM8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">PGG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_013981</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZFM8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026961">
        <property type="entry name" value="PGG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24186:SF37">
        <property type="entry name" value="PGG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24186">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13962">
        <property type="entry name" value="PGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="76"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="117"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="PGG" evidence="8">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9688022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="155" mass="17464" checksum="E1251004D8D5AC1C" modified="2024-01-24" version="1">MAYQAGLSPPGGVCQDEKPNSKGEIICYAGTSIMAFNYPVGYFKFMAYNTVSFVASLSMIFLLISGLPMKRRIFMWLLMVTMWVTITCMTLTYLISMQAVSPDLGNGAIIRVVTVSIWVWLGLIGIVMLIHTIRFLIWCVRKVRKLRKKKPSSLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZGF1</accession>
    <name>A0AA38ZGF1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_014075</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZGF1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070390">
        <property type="term" value="C:transcription export complex 2"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000973">
        <property type="term" value="P:post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000333">
        <property type="entry name" value="enhanced ethylene response protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045114">
        <property type="entry name" value="Csn12-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12732:SF0">
        <property type="entry name" value="PCI DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12732">
        <property type="entry name" value="UNCHARACTERIZED PROTEASOME COMPONENT REGION PCI-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00753">
        <property type="entry name" value="PAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="1">
        <location>
            <begin position="216"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9688167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="413" mass="47669" checksum="CB208D4B85788271" modified="2024-01-24" version="1">MAYLSMGEAHRRITEYLNRFSDAVLTQDGSSLKQLLSISSNSPLLLSLADALNLFHDSNRLLKQSDRFSQISEIVSPLFRSIQNFRLGNLLDSYNAFEKAANAFIQEFRNWESAWALEALYVIAYEIRVLAERADRELASVGKTPEKLKGAGSFLMKVFGVLAGKGPKRVGALYVTCQLFKIYFKLGTVHLCRSVIRSIETARIFDFEEFPIRDKVTYMYYTGRLEVFNENFPAADQKLSYALMHCNPHREANIRMILKYLIPVKLSIGILPKNWLLEKYNLIEYSNVVQALKRGDLRLLRHALQEHEDRFLRAGVYLVLEKLELQVYQRLVKKIYIIQKQKDPSKAHQLKLEVIVKALKWLEMDMDVDEVECIMSILIYKNLMKGYFAHKSKVVVLSKQDPFPKLNGKPVNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZJ75</accession>
    <name>A0AA38ZJ75_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PGG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_012485</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9689840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZJ75"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000679">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026961">
        <property type="entry name" value="PGG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24177">
        <property type="entry name" value="CASKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24177:SF314">
        <property type="entry name" value="PROTEIN ACCELERATED CELL DEATH 6-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13962">
        <property type="entry name" value="PGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="481"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="519"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="564"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="593"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="632"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="domain" description="PGG" evidence="3">
        <location>
            <begin position="471"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="294"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="294"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="307"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9689840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="696" mass="78405" checksum="6EB2149A5471BA1F" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZJC9</accession>
    <name>A0AA38ZJC9_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_012683</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NPH3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZJC9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043454">
        <property type="entry name" value="NPH3/RPT2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027356">
        <property type="entry name" value="NPH3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32370">
        <property type="entry name" value="OS12G0117600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03000">
        <property type="entry name" value="NPH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51649">
        <property type="entry name" value="NPH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="BTB" evidence="5">
        <location>
            <begin position="5"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="NPH3" evidence="6">
        <location>
            <begin position="189"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="167"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9690156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="533" mass="59364" checksum="651582ECFB0B8778" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZJS8</accession>
    <name>A0AA38ZJS8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peroxisomal membrane protein PMP22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_012408</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the peroxisomal membrane protein PXMP2/4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9689698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZJS8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007248">
        <property type="entry name" value="Mpv17_PMP22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266:SF86">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PMP22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04117">
        <property type="entry name" value="Mpv17_PMP22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="53"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="155"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9689698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="188" mass="21421" checksum="6205180B4D5385AB" modified="2024-01-24" version="1">MGSLAKKGLQQYLLQLQHHPLRTKAITAAVLSAVSDIVSQKLSGIQKLQLKRLLLKVLLGFVYLGPFGHFLHILLDKLFKGKKDSKTVAKKVVLEQLTASPWNNFVFMVYYGLVIEGRNWTQVKTKIKKDYPAVQYTSWTFWPVVGWVNHQYVPLQLRVIFHGVIACAWGIFLTLQARSISPKKAYVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZKZ1</accession>
    <name>A0AA38ZKZ1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_013263</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZKZ1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030132">
        <property type="term" value="C:clathrin coat of coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030130">
        <property type="term" value="C:clathrin coat of trans-Golgi network vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016025">
        <property type="entry name" value="Clathrin_H-chain_N"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50989">
        <property type="entry name" value="Clathrin heavy-chain terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9691011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="123" mass="14064" checksum="5557C9190D38A03C" modified="2024-01-24" version="1">MTSTKDSSRTVEFWVKEDEYERFFHGCRKHPYHHEGGSDVAERPITADATLMNPNTRILALKAQLSGTTADLKSIKSSSRNFEALQYPRRSSVSILIRAFRLTSPQCNFTSYGCCWLEERKGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZLC6</accession>
    <name>A0AA38ZLC6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">cysteine dioxygenase</fullName>
            <ecNumber evidence="3">1.13.11.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_013057</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-cysteine + O2 = 3-sulfino-L-alanine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20441"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:61085"/>
            <dbReference type="EC" id="1.13.11.20"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:20442"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cysteine dioxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.20" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZLC6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017172">
        <property type="term" value="F:cysteine dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070483">
        <property type="term" value="P:detection of hypoxia"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20289">
        <property type="entry name" value="cupin_ADO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012864">
        <property type="entry name" value="PCO/ADO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22966">
        <property type="entry name" value="2-AMINOETHANETHIOL DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22966:SF72">
        <property type="entry name" value="CYSTEINE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07847">
        <property type="entry name" value="PCO_ADO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9690702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="244" mass="27010" checksum="0B6B323B9C181753" modified="2024-01-24" version="1">MPVVQKLYNACKESFSVDGPLSEEALGKVRSILDDMKPSNVGLEQEAQLARGWKGSMHGANGKKVRNGSHQYPPPIKYLHLHECDRFSIGIFCMPPSSIIPLHNHPGMTVLSKLLYGTLHVKSYDWLDLPGTADLTQARPAKLVRDCEMSAPCGTTILYPTSGGNIHCFKAITPCALFDILSPPYSSEDGRHCSYFRKSPRKDLPGIDQLCGIKPSEVVWLEEIQPPENVVVLRGQYKGPIIRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZLS9</accession>
    <name>A0AA38ZLS9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_013209</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZLS9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037546">
        <property type="entry name" value="SAC51-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36066:SF11">
        <property type="entry name" value="TRANSCRIPTION FACTOR BHLH144"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36066">
        <property type="entry name" value="TRANSCRIPTION FACTOR BHLH145"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23173">
        <property type="entry name" value="bHLH_SAC51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="5">
        <location>
            <begin position="179"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9690942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="244" mass="27154" checksum="2E445EE994FD227B" modified="2024-01-24" version="1">MQRDQQFCPEKVMLPLANEVGNNYMYNTPVESAFGAVFPPGAKDLGPFHGVEFQPSDVCPKNFIIFDQTDHRSQIMFHPAIAQKFNCPSLNLCATYIHNNLEKREINIDEGEASSALKEDSEDIDALLSLEEEDQEEYDEEEVSTARTHGNYGSNCEDTCSSYGSKPRKIKLSSSILKSSSSGSSCNSERKRQKMKKMVKALRGIVPGSSQMNTVAVLDEAVRYLKSLKVEVQKLGVSNLKNIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZNW6</accession>
    <name>A0AA38ZNW6_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_011557</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZNW6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9692565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="144" mass="14901" checksum="C36682727E0874BD" modified="2024-01-24" version="1">MASNAQQKTDQAIANGRESSEVVKEKAQGAEDKTAETAQHAKDNTADTAQQAEGRTQESKDQSGCYLSQKAGAAKQKALEATEATKKKASEMIESARETAQAGKGKTGGILQTTGGKVKGMAQGAAGAVKQTFGTAGKEDRPKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZNX4</accession>
    <name>A0AA38ZNX4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_011384</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the peptidase M41 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZNX4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045037">
        <property type="term" value="P:protein import into chloroplast stroma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19501">
        <property type="entry name" value="RecA-like_FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000982">
        <property type="entry name" value="Inactive ATP-dependent zinc metalloprotease FTSHI 2 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000072">
        <property type="entry name" value="probable inactive ATP-dependent zinc metalloprotease FTSHI 2, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.760:FF:000012">
        <property type="entry name" value="probable inactive ATP-dependent zinc metalloprotease FTSHI 2, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.760">
        <property type="entry name" value="Peptidase M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000642">
        <property type="entry name" value="Peptidase_M41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037219">
        <property type="entry name" value="Peptidase_M41-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23076:SF56">
        <property type="entry name" value="INACTIVE ATP-DEPENDENT ZINC METALLOPROTEASE FTSHI 2, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23076">
        <property type="entry name" value="METALLOPROTEASE M41 FTSH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01434">
        <property type="entry name" value="Peptidase_M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00830">
        <property type="entry name" value="ENDOLAPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140990">
        <property type="entry name" value="FtsH protease domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="306"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="9">
        <location>
            <begin position="454"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="237"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="333"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9692299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="888" mass="100764" checksum="8474D5397C9EA94A" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZRR8</accession>
    <name>A0AA38ZRR8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Terpene cyclase/mutase family member</fullName>
            <ecNumber evidence="4">5.4.99.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_012079</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the terpene cyclase/mutase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.-" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9693179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZRR8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042300">
        <property type="term" value="F:beta-amyrin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016104">
        <property type="term" value="P:triterpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02892">
        <property type="entry name" value="SQCY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.20:FF:000011">
        <property type="entry name" value="Terpene cyclase/mutase family member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.20:FF:000064">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032696">
        <property type="entry name" value="SQ_cyclase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032697">
        <property type="entry name" value="SQ_cyclase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018333">
        <property type="entry name" value="Squalene_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002365">
        <property type="entry name" value="Terpene_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01787">
        <property type="entry name" value="squalene_cyclas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11764:SF58">
        <property type="entry name" value="BETA-AMYRIN SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11764">
        <property type="entry name" value="TERPENE CYCLASE/MUTASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13243">
        <property type="entry name" value="SQHop_cyclase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13249">
        <property type="entry name" value="SQHop_cyclase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01016">
        <property type="entry name" value="Prenyltransferase_Like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01074">
        <property type="entry name" value="TERPENE_SYNTHASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Squalene cyclase N-terminal" evidence="6">
        <location>
            <begin position="102"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="Squalene cyclase C-terminal" evidence="5">
        <location>
            <begin position="416"/>
            <end position="750"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9693179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="769" mass="88390" checksum="CD4F5455978874CD" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZSG6</accession>
    <name>A0AA38ZSG6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_010076</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZSG6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd17039">
        <property type="entry name" value="Ubl_ubiquitin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="1">
        <location>
            <begin position="66"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9694411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="148" mass="17129" checksum="05E08EF7B8D04D59" modified="2024-01-24" version="1">MMIVNFEIKGGEVIREIEMSTTSTILELKRSIECEMLKDVETIAHYGFHSAVGVHLMIMPKPEKEFFVVLKSLRPVANVKVKETNTVFDLKMKIQKLWGIETKNITLFRLFKEMEDDILLCAYYVYEGLEVEVEIAINKLLEIILLTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZTP5</accession>
    <name>A0AA38ZTP5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF7356 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_009928</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZTP5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055780">
        <property type="entry name" value="DUF7356"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34200">
        <property type="entry name" value="DENTIN SIALOPHOSPHOPROTEIN-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34200:SF2">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24053">
        <property type="entry name" value="DUF7356"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041386143" description="DUF7356 domain-containing protein" evidence="3">
        <location>
            <begin position="23"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="220"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7356" evidence="4">
        <location>
            <begin position="105"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="289"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="297"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF24053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9694183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="320" mass="35061" checksum="DA1E6C1A2BDF88A5" modified="2024-01-24" version="1">MDRNEFLPVIFLLFLVVNGSGASVADKFRKLANAEPKENSTATKIDPPPSPVSVGNQSEPNPIDPGKLGKEKSKDARVPTTNSSKADSNGKQEEKNDNKASSELGTYGTCDLLEKCTDPKKKMIACIQSFENGSKALIVQNEGENTLKVNLTVSDSVKDALGDLVIIPKHQTRSMNILSTGSSKLVLNAGNKDCVFENHLISEGNFFQWLPHSELVTPIYGVYFLFLTALVVGAMWTCCKFRKRRHQVGVPYQELEMGLPESSPAVNVETAAGWDEGWDDDWDEDKAVKSPGGQYVGSISSNGLTTRSSNREGWENDWDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZTP9</accession>
    <name>A0AA38ZTP9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Complex III subunit 9</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Complex III subunit X</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_010551</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UQCR10/QCR9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZTP9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045275">
        <property type="term" value="C:respiratory chain complex III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006122">
        <property type="term" value="P:mitochondrial electron transport, ubiquinol to cytochrome c"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.260:FF:000002">
        <property type="entry name" value="cytochrome b-c1 complex subunit 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.260">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008027">
        <property type="entry name" value="QCR9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036656">
        <property type="entry name" value="QCR9_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12980:SF0">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12980">
        <property type="entry name" value="UBIQUINOL-CYTOCHROME C REDUCTASE COMPLEX, SUBUNIT X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05365">
        <property type="entry name" value="UCR_UQCRX_QCR9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81514">
        <property type="entry name" value="Subunit X (non-heme 7 kDa protein) of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="72"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00076299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9695106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="95" mass="10645" checksum="AAAF392FD527C782" modified="2024-01-24" version="1">MDSTARRSGGGLLEGLYRVIMRRTSVYATFVILGAFAGERAVDYGVHKLWERNNVGVLQPTLLFCFLNVDQYLFLCTCQYLVLLGLLISFSCVLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZU20</accession>
    <name>A0AA38ZU20_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Homeobox-leucine zipper protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">HD-ZIP protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Homeodomain transcription factor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_010629</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Transcription factor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 10">Belongs to the HD-ZIP homeobox family. Class I subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZU20"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000385">
        <property type="entry name" value="Putative homeobox-leucine zipper protein ATHB-51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045224">
        <property type="entry name" value="HDZip_class_I_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000047">
        <property type="entry name" value="HTH_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24326">
        <property type="entry name" value="HOMEOBOX-LEUCINE ZIPPER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24326:SF591">
        <property type="entry name" value="HOMEOBOX-LEUCINE ZIPPER PROTEIN ATHB-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00031">
        <property type="entry name" value="HTHREPRESSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 8 9" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="55"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="8">
        <location>
            <begin position="57"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="107"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU369038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9695236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="220" mass="25853" checksum="5A698EBB96CDE8E0" modified="2024-01-24" version="1">MDWNGNLRPFVSRPESSFTFLYNYNYDPYPGMEVKHPVSAETAHAMVRPMDKSSYGNQEKKKRLTNEQLESLERSFQEEIKLEPDRKMKLARELGLQPRQIAVWFQNRRARWKTKELERLYDVLKQEYDLMSKEKQKLQEEVSKLKGILWEQATRKQVSMGYTEVSGEETVESTSITIRSSNKPRGLSHQIADCNYVYTAEEYNPVSPPYWGVLPPYPST</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZUU3</accession>
    <name>A0AA38ZUU3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Mur ligase central domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_010947</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZUU3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004326">
        <property type="term" value="F:tetrahydrofolylpolyglutamate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008764">
        <property type="term" value="F:UDP-N-acetylmuramoylalanine-D-glutamate ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="entry name" value="Mur ligase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="entry name" value="Mur-like, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00639">
        <property type="entry name" value="MurD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018109">
        <property type="entry name" value="Folylpolyglutamate_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004101">
        <property type="entry name" value="Mur_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005762">
        <property type="entry name" value="MurD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01087">
        <property type="entry name" value="murD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43692">
        <property type="entry name" value="UDP-N-ACETYLMURAMOYLALANINE--D-GLUTAMATE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43692:SF1">
        <property type="entry name" value="UDP-N-ACETYLMURAMOYLALANINE--D-GLUTAMATE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02875">
        <property type="entry name" value="Mur_ligase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51984">
        <property type="entry name" value="MurCD N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="MurD-like peptide ligases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="MurD-like peptide ligases, peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01011">
        <property type="entry name" value="FOLYLPOLYGLU_SYNT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mur ligase central" evidence="10">
        <location>
            <begin position="184"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="Mur ligase C-terminal" evidence="9">
        <location>
            <begin position="395"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9695721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="541" mass="58880" checksum="CCF86FEEF7D18175" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZV02</accession>
    <name>A0AA38ZV02_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_010349</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZV02"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005504">
        <property type="term" value="F:fatty acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009627">
        <property type="term" value="P:systemic acquired resistance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00010">
        <property type="entry name" value="AAI_LTSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.110.10">
        <property type="entry name" value="Plant lipid-transfer and hydrophobic proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036312">
        <property type="entry name" value="Bifun_inhib/LTP/seed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016140">
        <property type="entry name" value="Bifunc_inhib/LTP/seed_store"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039265">
        <property type="entry name" value="DIR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33122">
        <property type="entry name" value="LIPID BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33122:SF4">
        <property type="entry name" value="OS04G0415800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14368">
        <property type="entry name" value="LTP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47699">
        <property type="entry name" value="Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041297499" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical" evidence="2">
        <location>
            <begin position="25"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9694828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="118" mass="12263" checksum="B0AC0640991904C9" modified="2024-01-24" version="1">MEYPKSFIFLVGLLLVVSLASLEKVDGAGACGKSSPDNQALKLVPCATAASDKNAAVSSSCCLQVKKMIQNPSCLCAVVLSNTAKFAGVNPEIAITIPKRCNIADRPVGFKCGAYTLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZX16</accession>
    <name>A0AA38ZX16_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4283 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_008886</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZX16"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025558">
        <property type="entry name" value="DUF4283"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34427">
        <property type="entry name" value="DUF4283 DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34427:SF5">
        <property type="entry name" value="DUF4283 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14111">
        <property type="entry name" value="DUF4283"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4283" evidence="2">
        <location>
            <begin position="61"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="13"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="335"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="371"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="376"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9696885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="618" mass="67656" checksum="6C05550E6FA15987" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZY82</accession>
    <name>A0AA38ZY82_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_008984</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZY82"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9697017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="116" mass="13516" checksum="537C5E4B5328C044" modified="2024-01-24" version="1">MKMAIAALDVLHSYPATPRMKNLVPYCRLSLYMEGNNSLAFSGWIDSIQYCHFQVISGIVSMATCTKRFCNKITFFLHMHLFPYCFTIIFIFIFNSLHSFCEGGNWKVCHWELDKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A0X0</accession>
    <name>A0AA39A0X0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ENTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_007467</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Clathrin-coated vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A0X0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030125">
        <property type="term" value="C:clathrin vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03571">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276:SF116">
        <property type="entry name" value="ENTH_VHS FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276">
        <property type="entry name" value="EPSIN/ENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01417">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="6">
        <location>
            <begin position="38"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="208"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9698397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="337" mass="37825" checksum="B6743C43B3953308" modified="2024-01-24" version="1">MSSLGSNSGGRNMGAPFFHEFKKQASFFFKEKIKTARLALTDVTPAQLLTEEVTGGNPWSPDTRTMGLISRAAFEVDDYGRIVEILHNRLLRFDRKDWRASYNSLILLEHLLTHGPESVAGEFQSEKGVIEEMGSFQYIDEKGFNWGLTVRKKSERVSKLLQKGPLLKEERDRARKLTRGIQGFGSFTLKSSSAQGILRESSFHERCTSRLSDDENQLTSDEGSTRRGVGKSQQTQEDNAILAVGEKAENSNTWGSFSNGDVLKKCENQICFKENMVPVVRDLHEWSCTGESKPLLDGNRNEPRTAVSVDEDHPFNHTENQTTASLLSTRNEILQGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A1Y9</accession>
    <name>A0AA39A1Y9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_007915</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9699065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A1Y9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044653">
        <property type="entry name" value="AZF1/2/3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45988">
        <property type="entry name" value="C2H2 TYPE ZINC FINGER TRANSCRIPTION FACTOR FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45988:SF18">
        <property type="entry name" value="C2H2-TYPE ZINC FINGER FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="32"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9699065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="106" mass="11723" checksum="36437E51CBC925F8" modified="2024-01-24" version="1">MENKYVSSSHTSASKGEGLALGTSKQVVQKAHKCRTCNKTFPTGQALGGHQTTHRPKPAQFATPKHEALMLSSEEASQSTGPRVLDFDLNELPSMEEERMTIRIYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A318</accession>
    <name>A0AA39A318_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Alkaline/neutral invertase</fullName>
            <ecNumber evidence="6">3.2.1.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_007852</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Invertase that cleaves sucrose into glucose and fructose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.</text>
            <dbReference type="EC" id="3.2.1.26"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the glycosyl hydrolase 100 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.26" evidence="6"/>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A318"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033926">
        <property type="term" value="F:endo-alpha-N-acetylgalactosaminidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004575">
        <property type="term" value="F:sucrose alpha-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005987">
        <property type="term" value="P:sucrose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000001">
        <property type="entry name" value="probable alkaline/neutral invertase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024746">
        <property type="entry name" value="Glyco_hydro_100"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31916">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31916:SF15">
        <property type="entry name" value="ALKALINE_NEUTRAL INVERTASE D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12899">
        <property type="entry name" value="Glyco_hydro_100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5 6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9698984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="522" mass="59149" checksum="1EB8CDC7BAB6B131" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A430</accession>
    <name>A0AA39A430_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Germin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_008230</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May play a role in plant defense. Probably has no oxalate oxidase activity even if the active site is conserved.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Oligomer (believed to be a pentamer but probably hexamer).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Secreted</location>
            <location evidence="2 13">Extracellular space</location>
            <location evidence="2 13">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the germin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9699538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A430"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02241">
        <property type="entry name" value="cupin_OxOx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000098">
        <property type="entry name" value="Germin-like protein 9-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006045">
        <property type="entry name" value="Cupin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001929">
        <property type="entry name" value="Germin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31238">
        <property type="entry name" value="GERMIN-LIKE PROTEIN SUBFAMILY 3 MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00190">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00325">
        <property type="entry name" value="GERMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00835">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0052">Apoplast</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 11 13" id="KW-0464">Manganese</keyword>
    <keyword evidence="7 11 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Cupin type-1" evidence="14">
        <location>
            <begin position="32"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU366015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9699538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="185" mass="19524" checksum="236BCC183ED2498B" modified="2024-01-24" version="1">MAGDPNILSDFILPANQSTVDGSYFTYTGMRVLVGAPEPTSFKVLKASLAEFPALNGQSVSTAVLLFPADAVNPPHTHPRSAELLFLVYGSLQVGFVDTTNNLYTQTLQAGDMFVFPKGLVHFQYNVDSKYSAAAVSAFGSANAGTVSVPNSVFTTGIDDNILAKSFKTDVATIQAIKAGLTPKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A7V6</accession>
    <name>A0AA39A7V6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SHSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_004018</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A7V6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06464">
        <property type="entry name" value="ACD_sHsps-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.790:FF:000010">
        <property type="entry name" value="17.3 kDa class II heat shock protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031107">
        <property type="entry name" value="Small_HSP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11527">
        <property type="entry name" value="HEAT-SHOCK PROTEIN 20 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="6">
        <location>
            <begin position="38"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9702072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="154" mass="17836" checksum="F431D804F2B4EB76" modified="2024-01-24" version="1">MDLNNIGFDSSMLMALQDMLDMYEEPVRHATSRSYLRDGKAMAATQADLKEYPNAYVFLVDMPGLKAYKIKVHIEDENMLVVYGERKQDKKEMVKYLKIERRFGKFLKRFVLAENADMDAISAIYQDGVLTVTVEKKYPPETKPRKTIEVRVGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A8C1</accession>
    <name>A0AA39A8C1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_004353</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A8C1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9702574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="118" mass="13056" checksum="8E41684A874FD36F" modified="2024-01-24" version="1">MAVSLPRQSPTTASSSLSIEVPENQSQPFSCVVTSTADPKKSLLDLPFFIAASRTESSPIMESPVSHGVDTPVVSLGMMKMERRGLRVGFQWKERMMKEKKVLWVGRSRTSTNVGRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A9P3</accession>
    <name>A0AA39A9P3_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_005075</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A9P3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23700">
        <property type="entry name" value="At3g51010"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36767">
        <property type="entry name" value="OS05G0126200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36767:SF1">
        <property type="entry name" value="OS05G0126200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9703593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="182" mass="21097" checksum="CC2EE6FDDFD03127" modified="2024-01-24" version="1">MGLGALRSIIRPISRTLVTRTSTLYTTSFGASSPSPRPDFRFVSGSLHWLPRWVPVANHFHSLTDTRLPKRRPSDKPRRKRASLRPPGPYAWVQYVPGERILPNRPNEGSVKGRKEKKRMRLRRAFILSEAKKRKAQLQEANRKKNIKRVERKMAAVARERAWAQRLAELQQLEEEKKKSVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ABU0</accession>
    <name>A0AA39ABU0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_002959</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ABU0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052788">
        <property type="entry name" value="RING-type_E3_ligase_ATL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45798">
        <property type="entry name" value="RING-H2 FINGER PROTEIN ATL61-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45798:SF101">
        <property type="entry name" value="RING-H2 FINGER PROTEIN ATL8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="29"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="103"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9704657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="154" mass="16621" checksum="F1B62D3B82B72A48" modified="2024-01-24" version="1">MPLLDKLLAGGNSIAAEPPTPAKMEGYNIFISVAFVSALICAVGLLVVGHCVCLRYFYRHGGGNTAASSSTEQSLPNGGPKEERILSSVPRVRYTGDGKVGECAICLTEFMKGDEMSVMPQCGHGFHVKCIERWMRRSSSCPSCRQILVEPSRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ACY2</accession>
    <name>A0AA39ACY2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Serine carboxypeptidase-like 18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_003383</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ACY2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019748">
        <property type="term" value="P:secondary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12670:FF:000002">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000072">
        <property type="entry name" value="Serine carboxypeptidase-like 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12670">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF329">
        <property type="entry name" value="SERINE CARBOXYPEPTIDASE-LIKE 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041318480" description="Serine carboxypeptidase-like 18" evidence="2">
        <location>
            <begin position="28"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9705318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="471" mass="53774" checksum="04192FA4A1409ECB" modified="2024-01-24" version="1">MLFSPNSSCSGCVFLSLLLLLLLSATAVFCGSIVRYLPGFDGELPFKLETGYVRVDEIEIFYYFIESERNPRMDPIMLWYSGGPGCSAQNGLFYEIGPLAFNITEYYGGLPSLEYYPYSWTKTASILFADVPVGTGFSYTTDPSSWSTSDTTTAQQTYIFLKKWLIDHPQFLQVQLFVGADSYSGITAPMVVQHILDGNNDGDTPHLNLKGYIVGCPRTDAGINENSKIKFLHRMGFISDELYESAKESCGGQYYEADFYTPQCTEDVKLIEKGTHDLDKNNILDPKCTWASPQTDGDTKQRSLEENPRNFLLSPPIIPDFWCHNFNYALSYTWANDVRVQKALQVREGTIEDWKRCNKSIPYKMDQKSVIGFHHNLSRYGLQVLVYNGDHDLTIPNVGTQEWIELLNITVVNDWRPWLVDGEIAGYTIKYSEHGYRLTYATIKGAGHSPQEYKRRECFEMFHRFISYYPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AD17</accession>
    <name>A0AA39AD17_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">glucan endo-1,3-beta-D-glucosidase</fullName>
            <ecNumber evidence="4">3.2.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_003043</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;3)-beta-D-glucosidic linkages in (1-&gt;3)-beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.39"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the glycosyl hydrolase 17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.39" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9704810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AD17"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000005">
        <property type="entry name" value="Glucan endo-1,3-beta-glucosidase 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1040:FF:000003">
        <property type="entry name" value="glucan endo-1,3-beta-glucosidase 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000490">
        <property type="entry name" value="Glyco_hydro_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044965">
        <property type="entry name" value="Glyco_hydro_17_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012946">
        <property type="entry name" value="X8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32227">
        <property type="entry name" value="GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00332">
        <property type="entry name" value="Glyco_hydro_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07983">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00768">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041324677" description="glucan endo-1,3-beta-D-glucosidase" evidence="12">
        <location>
            <begin position="27"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="domain" description="X8" evidence="13">
        <location>
            <begin position="403"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="377"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="385"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9704810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="490" mass="52448" checksum="A7CBAE510FBB1980" modified="2024-01-24" version="1">MPTLSHASIFFFFFSLLFPSLHFAIGFQWSGGTRVHEWVFGSIMGQKLERRGLDSQSFIGINYGQVANNLPPPEATAKLLKSTSIEKVRLYGADPGIIKALANTGIGIVIGAANGDIPALASDPNFAAQWVNSNVIAYYPASKIILITVGNEVMSSTDKNLMSQLLPAMQNVQNVLNAASLGGKIKVSTVHSMAVLTQSDPPSSGAFDPATSDTMRGLLEFQRNTGSPFAINPYPFFAYQSDPRPETLAYCLFQPNSGRVDSGNNIKYMNMFDAQVDAVRSALNAMGFKEVEIVVAETGWPYSGDANEVGPSIENAKAYNGNLIAHLRSMVGTPLMPGKSVDTYIFALYDENLKPGPGSERAFGLFKPDLSMTYDVSLSKSSPTPSTPETPVNPSPNPTASSGWCVPKAGISDAQLQTNLDYACGQGIDCTPIQPGGACFEPNTVASHAAYAMNLLYQSSGKNPWNCDFTQTAMLTSNNPSYNACTFPGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ADW9</accession>
    <name>A0AA39ADW9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclin-dependent kinase inhibitor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PVL29_003703</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CDI family. ICK/KRP subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ADW9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004861">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003175">
        <property type="entry name" value="CDI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044275">
        <property type="entry name" value="KRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46776">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE INHIBITOR 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02234">
        <property type="entry name" value="CDI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017811">
        <property type="entry name" value="CDK_inhib_pln"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0649">Protein kinase inhibitor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-dependent kinase inhibitor" evidence="4">
        <location>
            <begin position="163"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR017811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ9705737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="202" mass="22977" checksum="0EC134EF20FE55DC" modified="2024-01-24" version="1">MEKYMNECETIIGIAMMKVGHGSVRVRPVEASTTDASKRKRIAFGELQMSPSSNLELRSNCCYVNLPPQSENYFKQVLSDLCTSSCVDHAPEPLCSSNELGEVVRSSLRSVDLEAKGFDTENPTYSDGRFSRETTPMSELCVDSAEMESPAKTIAMSSIVKIKVFYFAAEKYQQWRFTEKYNYDIVKNAPMECRYQWVHLEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AF89</accession>
    <name>A0AA39AF89_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_003862</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AF89"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000400">
        <property type="entry name" value="Nuclear pore complex protein NUP107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053213">
        <property type="entry name" value="RLP29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48009">
        <property type="entry name" value="LEUCINE-RICH REPEAT (LRR) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48009:SF1">
        <property type="entry name" value="LEUCINE-RICH REPEAT (LRR) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041335386" description="Leucine-rich repeat-containing N-terminal plant-type domain-containing protein" evidence="6">
        <location>
            <begin position="25"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="7">
        <location>
            <begin position="36"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9705950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="403" mass="43005" checksum="6D1E3F28E7F20303" modified="2024-01-24" version="1">MAPSPSSSPSFLLIFSIFFVLFHAAPSFQLQLNSLDISALLAIKNTLTDVPGTHFFSSWDFASPRPCSTFAGVICSLDNSSPNPTLRVTSLTLGTGLSDSPGLAGSLSASLSNLTQLTQLVLYAGLVTGPIPPQLGNLINLRVLSLTNNRLTGPIPASLSSLHNLHTLDLSFNQLSGSIPPGIAHLDQLKVLILASNILSGELPKFPAIQELFHIDVKRNKLWGRLPSSMPVSLRYLSLVYLDLSMNQFSGPIPKALFHSRLNSMLLQRNNFSGGVPTAASSTSYAQGSIVDLSHNLLTGELSPILAGVETLFLNNNRLIGRVPDEYVNSISRGDTKTLYLQHNYISDFPMQSGLALPDSVSLCLSYNCMVPPVGLAACPASAGGQLSRPAYQCSVYNNGTAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AGM0</accession>
    <name>A0AA39AGM0_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_002313</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AGM0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045344">
        <property type="entry name" value="C-JID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058546">
        <property type="entry name" value="RPS4B/Roq1-like_LRR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47186">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47186:SF61">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 57-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20160">
        <property type="entry name" value="C-JID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23286">
        <property type="entry name" value="LRR_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance protein RPS4B/Roq1-like leucine-rich repeats" evidence="6">
        <location>
            <begin position="123"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="C-JID" evidence="5">
        <location>
            <begin position="328"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="490"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="498"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF20160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9707271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="513" mass="57732" checksum="DC65724B4C404446" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AIP1</accession>
    <name>A0AA39AIP1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_002220</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AIP1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36617">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36617:SF15">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9707128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="126" mass="14486" checksum="45B960E8F7FFD97A" modified="2024-01-24" version="1">MGHLVTPSFGFVVGDGKKVGFWKDKWCGTIPLCEAFPSLYVLASYKEAWVNEVWTAEGERGGSWNLCFNRPFNDWELEEVERLFCCLEGKKVRVDEEDMVRWMDSKDGVFSVKSLYKTMQPVSLVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AJP8</accession>
    <name>A0AA39AJP8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein-tyrosine-phosphatase MKP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_000668</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AJP8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057528">
        <property type="entry name" value="MPK1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007122">
        <property type="entry name" value="Villin/Gelsolin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46381:SF2">
        <property type="entry name" value="MAP KINASE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46381">
        <property type="entry name" value="MKPA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25466">
        <property type="entry name" value="MPK1_gelsolin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00262">
        <property type="entry name" value="GEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="4">
        <location>
            <begin position="116"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="5">
        <location>
            <begin position="175"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="66"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="542"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="577"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="545"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="577"/>
            <end position="591"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9708766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="876" mass="97014" checksum="45F2DAB72851C5C4" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AK50</accession>
    <name>A0AA39AK50_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_000520</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Nudix hydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AK50"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016462">
        <property type="term" value="F:pyrophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04666">
        <property type="entry name" value="NUDIX_DIPP2_like_Nudt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000022">
        <property type="entry name" value="Nudix hydrolase 17, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047198">
        <property type="entry name" value="DDP-like_NUDIX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12629">
        <property type="entry name" value="DIPHOSPHOINOSITOL POLYPHOSPHATE PHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12629:SF62">
        <property type="entry name" value="NUDIX HYDROLASE 17, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0464">Manganese</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="25"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="Nudix hydrolase" evidence="9">
        <location>
            <begin position="57"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9708522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="198" mass="23251" checksum="0CBB2A4FCDBF8C83" modified="2024-01-24" version="1">MSVWTSVSFEMGRCLIEKRGWPPYAFLWSFSFYIPFVDFLNLNILFPVIFKFCRCIPYRYKTGSDGSIKDELEVLVISSKKGQGMMFPKGGWETDESVEEAASRESLEEAGVLGKVGCELGQWSFMSKRYGTFYEGYMFPLLVKEQLDLWPEKNERQRIWMDVAEAREVCQHWWMKEALDVLVRRLTTLPRHPQEDVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AKV5</accession>
    <name>A0AA39AKV5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PGG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_001151</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AKV5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026961">
        <property type="entry name" value="PGG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24177:SF292">
        <property type="entry name" value="ANKYRIN REPEAT FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="5"/>
    </dbReference>
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        </source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
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        <name type="common">Muscadine grape</name>
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        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
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                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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            <end position="28"/>
        </location>
    </feature>
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            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAJ9708373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="89" mass="10341" checksum="8EFE4289974387B3" modified="2024-01-24" version="1">MMMAYIFNAMTHLAVDVTLMAFTFFNLVKNQFYLKDKGKLKNALARLVSCLTLLPCNTRKVVSSFEENLARKRVLHVFEPDLPKDPERF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
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    <name>A0AA39APK6_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ORF">PVL29_001215</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
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                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9709618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="140" mass="15831" checksum="8CE3311642CBDB7F" modified="2024-01-24" version="1">MILNGDSLVSKKEVLSYGILITKICQMVGVEFPTNSTFLKPMGPINTLSWNRSKGQIEGALFPRKKVCRPRDKNTIGEAQLSARFDALEVKISTQSTLFSKQFSQLQSHMEKGFFLIDGQMLYNMDHLQALEDLVHKHLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
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    <name>A0AA39D5J8_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
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    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
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                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22249">
        <property type="entry name" value="UDM1_RNF168_RNF169-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051195">
        <property type="entry name" value="Fungal_stress_NST1"/>
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    <dbReference type="PANTHER" id="PTHR31780:SF10">
        <property type="entry name" value="LD36051P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31780">
        <property type="entry name" value="STRESS RESPONSE PROTEIN NST1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
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            <end position="142"/>
        </location>
    </feature>
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        </location>
    </feature>
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            <end position="443"/>
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        <location>
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            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
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            <end position="774"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="802"/>
            <end position="829"/>
        </location>
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            <end position="1096"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
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            <end position="1665"/>
        </location>
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            <end position="1746"/>
        </location>
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        <location>
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            <end position="2144"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2398"/>
            <end position="2490"/>
        </location>
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    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="594"/>
            <end position="711"/>
        </location>
    </feature>
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        <location>
            <begin position="86"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="118"/>
            <end position="128"/>
        </location>
    </feature>
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        </location>
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        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
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            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
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        </location>
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        <location>
            <begin position="754"/>
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        </location>
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        </location>
    </feature>
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            <end position="1017"/>
        </location>
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            <end position="1038"/>
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            <begin position="1204"/>
            <end position="1217"/>
        </location>
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        <location>
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        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="2481"/>
            <end position="2490"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9670765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="2490" mass="269386" checksum="83ED753A2E99D6FC" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D7D0</accession>
    <name>A0AA39D7D0_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_026126</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
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            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D7D0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9672775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="124" mass="13246" checksum="4D3C0A9BEE8FD1E1" modified="2024-01-24" version="1">MDTPALLYVEKIGRGVSECMLSGRLFPRYGQLTSCSVPSCFPKVLPALSTSAGVPDGNEAKFKYAPHDSHIISSISTFIQPFRAQANELATLGFPNQFDNATDHSLRGCGIGCHSEAGGMLLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DA73</accession>
    <name>A0AA39DA73_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_022516</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">RNA helicase that plays an essential role in pre-mRNA splicing as component of the U5 snRNP and U4/U6-U5 tri-snRNP complexes. Involved in spliceosome assembly, activation and disassembly.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9677573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18020">
        <property type="entry name" value="DEXHc_ASCC3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18022">
        <property type="entry name" value="DEXHc_ASCC3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000198">
        <property type="entry name" value="Activating signal cointegrator 1 complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3380.10:FF:000002">
        <property type="entry name" value="Activating signal cointegrator 1 complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000231">
        <property type="entry name" value="Activating signal cointegrator 1 complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000207">
        <property type="entry name" value="DExH-box ATP-dependent RNA helicase DExH14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000004">
        <property type="entry name" value="RNA helicase, activating signal cointegrator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000102">
        <property type="entry name" value="RNA helicase, activating signal cointegrator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000012">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000024">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000004">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3380.10:FF:000001">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000062">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.10">
        <property type="entry name" value="Sec63 N-terminal domain-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056379">
        <property type="entry name" value="DExH14_plug"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050474">
        <property type="entry name" value="Hel308_SKI2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004179">
        <property type="entry name" value="Sec63-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057842">
        <property type="entry name" value="WH_MER3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961:SF13">
        <property type="entry name" value="ACTIVATING SIGNAL COINTEGRATOR 1 COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961">
        <property type="entry name" value="DNA POLYMERASE THETA, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G05260)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24557">
        <property type="entry name" value="DExH14_plug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02889">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23445">
        <property type="entry name" value="WHD_SNRNP200"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039073">
        <property type="entry name" value="BRR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00973">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158702">
        <property type="entry name" value="Sec63 N-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="450"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="12">
        <location>
            <begin position="667"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="1298"/>
            <end position="1473"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="12">
        <location>
            <begin position="1504"/>
            <end position="1713"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="309"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="309"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="326"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00055371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9677573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="2092" mass="237201" checksum="47809BC79F93C1F5" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DA77</accession>
    <name>A0AA39DA77_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CMP/dCMP-type deaminase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_025087</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytidine and deoxycytidylate deaminase family. ADAT3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9676389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DA77"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052717">
        <property type="term" value="F:tRNA-specific adenosine-34 deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002100">
        <property type="term" value="P:tRNA wobble adenosine to inosine editing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11079">
        <property type="entry name" value="CYTOSINE DEAMINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11079:SF156">
        <property type="entry name" value="INACTIVE TRNA-SPECIFIC ADENOSINE DEAMINASE-LIKE PROTEIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="3">
        <location>
            <begin position="262"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9676389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="425" mass="47553" checksum="A018F9A382AB0F28" modified="2024-01-24" version="1">MERAKWEITHIPEKPPVSPTHQPTVDVLASVVDPKLANTLVRRLNQIAPLENFCHVKRIQKKQMQGGKTQLAIILCLASENENPLDGIPNDVLELINSYQLSAFITKVCKYAALSKEEWEEQCKLWPTSYHPPTYNIEGITGFSEEDSQSVFSFMKSAVKLAKSDDGQVVNAAVIVDPSVKQIIVSACDEICSWHTPMDKTSVEACCLEQPDIATSQGVVNGVVSHETLLSKGPPDEPKQLYTGVSCLYPWRWAEHQSHASSYWHPLRHAAIVAIEYSAARDRRLFPGLGQIGNQSNQLDHTLSASISSQAKRQKTNLKKVEDVEKLDAHYNGFHSDAARPYLCTGYDIYLVWEPCAMCAMALVHQRIRRIFYAFPNPNAGALGSVHRLQGEKSLNHHYAVFRVSLPEEILDGGDHHFDIHINLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DAF0</accession>
    <name>A0AA39DAF0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein DETOXIFICATION</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Multidrug and toxic compound extrusion protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_022073</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9676886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DAF0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13132">
        <property type="entry name" value="MATE_eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045069">
        <property type="entry name" value="MATE_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="44"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="81"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="124"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="223"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="263"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="301"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="341"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="384"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="414"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="444"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9676886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="508" mass="55076" checksum="96C224392D13AE7D" modified="2024-01-24" version="1">MDTGDEKPTLTSPLIQSCGEDGGERSKGKLFQRDKVVEEVKKQLWLAGPLISVSLLQYCLQLISVMFVGHLGELALSGASMATSFASVTGSSLLMGMASALDTLCGQSYGAKQYHMLSIHMQRAMLILLIVSVPLATIWAYTGTILKAVGQDPEISQEAELYARFMIPSLFAYGLLQCLVRFLQTQNIVFPMMLSSGITTLLHVLVCWFLVFKSGLGTKGAALANSISCWINVLLLALYVKFSSSCSKTRTGFSKEALHNIPSFLRLAIPSAVMVCLEMWSFELMVLLSGLLPNPKLETSVLSICLNTAATVWMIPFGLSGAVSTRVSNELGAGHPQAARLAAFVVLVMAITEGTLIGLLLILIRNIWGYAYSNEIEVVKYVAAMMPILALSNFLDGMQCVLSGTARGCGWQKIGAFVNLGSYYIVGIPCAILLAFVFHIGGRGLWLGIICALIVQVLSLFIVTLRTNWEAEVKHSFLLFISVLESPFVSPPPTEVSPPFCLGKEGYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DCT9</accession>
    <name>A0AA39DCT9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Acetolactate synthase</fullName>
            <ecNumber evidence="4 14">2.2.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PVL29_021078</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>2 pyruvate + H(+) = (2S)-2-acetolactate + CO2</text>
            <dbReference type="Rhea" id="RHEA:25249"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58476"/>
            <dbReference type="EC" id="2.2.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="14">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
        <text evidence="14">Binds 1 thiamine pyrophosphate per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 14">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the TPP enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.6" evidence="4 14"/>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DCT9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005948">
        <property type="term" value="C:acetolactate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003984">
        <property type="term" value="F:acetolactate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009635">
        <property type="term" value="P:response to herbicide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02015">
        <property type="entry name" value="TPP_AHAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07035">
        <property type="entry name" value="TPP_PYR_POX_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1220:FF:000008">
        <property type="entry name" value="Acetolactate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000007">
        <property type="entry name" value="Acetolactate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012846">
        <property type="entry name" value="Acetolactate_synth_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039368">
        <property type="entry name" value="AHAS_TPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000399">
        <property type="entry name" value="TPP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045229">
        <property type="entry name" value="TPP_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00118">
        <property type="entry name" value="acolac_lg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968:SF13">
        <property type="entry name" value="ACETOLACTATE SYNTHASE CATALYTIC SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968">
        <property type="entry name" value="THIAMINE PYROPHOSPHATE ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00187">
        <property type="entry name" value="TPP_ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 14" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="12 14" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0274">FAD</keyword>
    <keyword evidence="9" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0359">Herbicide resistance</keyword>
    <keyword evidence="10 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 14" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="7 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme N-terminal TPP-binding" evidence="17">
        <location>
            <begin position="76"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme central" evidence="15">
        <location>
            <begin position="267"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="16">
        <location>
            <begin position="475"/>
            <end position="630"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ9679044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="656" mass="71671" checksum="92F525638AE5840D" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DDI7</accession>
    <name>A0AA39DDI7_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_023012</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9678277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DDI7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9678277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="73" mass="8591" checksum="11820E24E0CFCE2B" modified="2024-01-24" version="1">MKISIFLCFRENFKMGNQKRKSIEFEEEFMEISDENGDDTGLELPPRFSLSEPPIFEPPTQRYITRTSLDKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DDX0</accession>
    <name>A0AA39DDX0_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_019443</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DDX0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9680155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="96" mass="10538" checksum="E4C49084AA4B527C" modified="2024-01-24" version="1">MKDLADLFTKLMSKAALSNFQTKLCLQPRHSLRKGIGTTQHLGVNCGTQRSDKVKHNYESDKVENSHKSCYNSGETLKDEGVSMATTSTRNGSSTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DE25</accession>
    <name>A0AA39DE25_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_019643</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the RING-type zinc finger family. ATL subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DE25"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16461">
        <property type="entry name" value="RING-H2_EL5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044600">
        <property type="entry name" value="ATL1/ATL16-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46913">
        <property type="entry name" value="RING-H2 FINGER PROTEIN ATL16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46913:SF1">
        <property type="entry name" value="RING-H2 FINGER PROTEIN ATL16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="63"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9680379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="153" mass="17373" checksum="8E3C00783EFA407A" modified="2024-01-24" version="1">MGDRLELAGDTVVWTVASAVTTHPTVVIAITITMAFGYYRHKVLRRDHVFCYKPNRTHQQTYCVVCLYDAVEGERLRRLPDCKHCFHVGCIDAWFQAHSTCPLCRSQVSLPHRRQQTLFSHFVSVLKLIADKTGNPLCLEIPLALCDSLGYIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DFE7</accession>
    <name>A0AA39DFE7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AUGMIN subunit 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_019485</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DFE7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070652">
        <property type="term" value="C:HAUS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051011">
        <property type="term" value="F:microtubule minus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031023">
        <property type="term" value="P:microtubule organizing center organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051225">
        <property type="term" value="P:spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029711">
        <property type="entry name" value="Haus7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010604">
        <property type="entry name" value="Plant_AUG7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14352">
        <property type="entry name" value="HAUS AUGMIN-LIKE COMPLEX SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14352:SF2">
        <property type="entry name" value="HAUS AUGMIN-LIKE COMPLEX SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06694">
        <property type="entry name" value="Plant_NMP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028655">
        <property type="entry name" value="Plant_NMP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="171"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9680197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="332" mass="37642" checksum="91A6562DBB28A600" modified="2024-01-24" version="1">MAAKQMEEIQKKLGMLNYPRANAPAQSLLFAGMERYALLEWLFFRLLGDKSPFSQQNLQGENGMDRDEETARIQYLAEIAKFLGITTTVDTEAIQGRGTYEDRTEMLRLIVDLVEASIYADNPEWSVDEQVAKDIQLIDSIAERQAQIFSEECKLFPADVQIQSKYLLPDISELETKLSEQSKLLLNLQQKVDDLASKHAYNPDEEYEEVESQLRAHLESFLETARSFNVIYTKEIRPWTHMMEVPQLHGFGPAANRLLEAYKMLLKFLGNLRNLRDSHAAVAVGSSETIAGQPSPVTRIISECESALTFLNRDLGILSASIAREQGEEFTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DG78</accession>
    <name>A0AA39DG78_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4283 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_018713</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DG78"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025558">
        <property type="entry name" value="DUF4283"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34427">
        <property type="entry name" value="DUF4283 DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34427:SF5">
        <property type="entry name" value="DUF4283 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14111">
        <property type="entry name" value="DUF4283"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4283" evidence="2">
        <location>
            <begin position="62"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="258"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="292"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="318"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9682841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="601" mass="67315" checksum="1E5A26E8C10CB698" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DGS5</accession>
    <name>A0AA39DGS5_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_016686</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DGS5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.50:FF:000006">
        <property type="entry name" value="Fringe-related protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006740">
        <property type="entry name" value="DUF604"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10811">
        <property type="entry name" value="FRINGE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04646">
        <property type="entry name" value="DUF604"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9684323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="492" mass="55843" checksum="4E6813CA0373242D" modified="2024-01-24" version="1">MSQARETSYGPFKACNFVVFSIEKPSENLVRVIRCAILIFLVTSFSLVLYSAVTSQTRWFPFPEGADQYSGGPYGTTDYTSGDNLGETNISHVQFGIAGSVATWKNRRHYSELWWKPNVSRGYVWLDGKPDAAVPWPKSSPPYRVSEDWSRFKYSSSQSAVRIARIVSESFRVGLPNVRWFVMGDDDTVFFTENLVSVLAKYDHRQVYYIGANSESVEQDVMHSYGMAFGGGGFAVSYGLAAKLATMLDGCLDRYYRFYGSDQRIWACVSEIGVSLTMERGFHQIDIRGDPYGLLAAHPVAPLVSLHHIDAVRPMFPNHTHLDSLKSLFRAYQVDPARILQQSFCYDRRRNWSISVSWGYTAQLYPWLVPAHILDTPLQTFQTWRSRSQGPFTFNTRKVTSDPCEQPVIYFLEEVQEVAKGETMSSYGRAVVQPGKACQRPDYAPVMAVQRIKVSTLKMSPQDWKKAPRRQCSEIVKLKDSTLQVKIRSCKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DGU5</accession>
    <name>A0AA39DGU5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_017864</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9681682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DGU5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005689">
        <property type="term" value="C:U12-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001849">
        <property type="entry name" value="Zinc finger CCCH domain-containing protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013085">
        <property type="entry name" value="U1-CZ_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16465">
        <property type="entry name" value="NUCLEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16465:SF0">
        <property type="entry name" value="ZINC FINGER MATRIN-TYPE PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06220">
        <property type="entry name" value="zf-U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="52"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="52"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="125"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="134"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9681682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="152" mass="16836" checksum="927C64160ED72D76" modified="2024-01-24" version="1">MPLGKYHCDYCDKQFQDTPSARKRHLQGLQHQRAKALWYDSFRDPNQIQAEGYGKGVCNRFVRTGFCQYGDSCKYIHPKQSLQNTNTQSVPVTGFPDNIQSLSNPGNQLVGGGPLPGNVVKDSMGNSWGNLPPSLNPPPEGGYPPLPFLDWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DHK8</accession>
    <name>A0AA39DHK8_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_018392</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DHK8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9682462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="87" mass="10152" checksum="1C3C15B51EE02667" modified="2024-01-24" version="1">MELSIANHGAKNDLIIDYDKNSIKPIQESMMINTTPIKISARDKKKEEKNAKPTQENERCWFSLKELEEKKYHFPDSDVPSMLEDLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DL01</accession>
    <name>A0AA39DL01_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Glutamate carboxypeptidase LAMP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PVL29_014143</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M28 family. M28B subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DL01"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08022">
        <property type="entry name" value="M28_PSMA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02121">
        <property type="entry name" value="PA_GCPII_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000008">
        <property type="entry name" value="Glutamate carboxypeptidase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.930.40:FF:000001">
        <property type="entry name" value="N-acetylated-alpha-linked acidic dipeptidase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000009">
        <property type="entry name" value="N-acetylated-alpha-linked acidic dipeptidase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.40">
        <property type="entry name" value="Transferrin receptor-like, dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039373">
        <property type="entry name" value="Peptidase_M28B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007365">
        <property type="entry name" value="TFR-like_dimer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036757">
        <property type="entry name" value="TFR-like_dimer_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10404">
        <property type="entry name" value="N-ACETYLATED-ALPHA-LINKED ACIDIC DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10404:SF46">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04253">
        <property type="entry name" value="TFR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47672">
        <property type="entry name" value="Transferrin receptor-like dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041223054" description="Glutamate carboxypeptidase LAMP1" evidence="15">
        <location>
            <begin position="27"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="16">
        <location>
            <begin position="141"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M28" evidence="18">
        <location>
            <begin position="325"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="domain" description="Transferrin receptor-like dimerisation" evidence="17">
        <location>
            <begin position="572"/>
            <end position="699"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ9688301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="21">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="704" mass="77521" checksum="509B739A256AB8E0" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DLF2</accession>
    <name>A0AA39DLF2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Inositol oxygenase</fullName>
            <ecNumber evidence="4 12">1.13.99.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Myo-inositol oxygenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PVL29_014124</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>myo-inositol + O2 = D-glucuronate + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23696"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
            <dbReference type="ChEBI" id="CHEBI:58720"/>
            <dbReference type="EC" id="1.13.99.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11 12">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="11 12">Binds 2 iron ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Polyol metabolism; myo-inositol degradation into D-glucuronate; D-glucuronate from myo-inositol: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the myo-inositol oxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.13.99.1" evidence="4 12"/>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DLF2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050113">
        <property type="term" value="F:inositol oxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019310">
        <property type="term" value="P:inositol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019853">
        <property type="term" value="P:L-ascorbic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007828">
        <property type="entry name" value="Inositol_oxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12588:SF14">
        <property type="entry name" value="INOSITOL OXYGENASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12588">
        <property type="entry name" value="MYOINOSITOL OXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05153">
        <property type="entry name" value="MIOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0060">Ascorbate biosynthesis</keyword>
    <keyword evidence="5 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 11 12" id="KW-0408">Iron</keyword>
    <keyword evidence="7 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="10">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="107"/>
            <end position="109"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="166"/>
            <end position="167"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="244"/>
            <end position="245"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR607828-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR607828-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU367039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ9688254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="309" mass="35911" checksum="15EBBFFFDED53CFE" modified="2024-01-24" version="1">MTIIIDQPELGSQPEKLSSETNELVLDGGFPVPKDGFMAPEINSFGNSFRDYDAKSERQKTVEEFYRINHINQTYDFVKQMREAYGKLDRVEMSIWECCELLNDVIDDSDPDLDEPQIEHLLQTAEAIRKDYPNEDWLHLTGLIHDLGKVLLHPSFGELPQWAVVGDTFPLGCAFDEANVHHKYFKENPDYNNPDYSTKNGIYSEGCGLDNVMISWGHDDYMYLVAKENKTTLPPAALFVIRYHSFYPLHRHGAYKHLMNEEDVENLKWLHIFNKYDLYSKSKVRIDVEKVKPYYLSLIEKVKPPLLIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DN46</accession>
    <name>A0AA39DN46_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Bromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_013144</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DN46"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd05506">
        <property type="entry name" value="Bromo_plant1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052442">
        <property type="entry name" value="Env_Response_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037377">
        <property type="entry name" value="GTE_bromo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027353">
        <property type="entry name" value="NET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038336">
        <property type="entry name" value="NET_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46136:SF19">
        <property type="entry name" value="TRANSCRIPTION FACTOR GTE12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46136">
        <property type="entry name" value="TRANSCRIPTION FACTOR GTE8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17035">
        <property type="entry name" value="BET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="3 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Bromo" evidence="10">
        <location>
            <begin position="67"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="167"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="279"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="434"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="179"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="283"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="308"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9690848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="572" mass="64045" checksum="FAA561D4B31C1936" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DSZ3</accession>
    <name>A0AA39DSZ3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Thioredoxin reductase</fullName>
            <ecNumber evidence="8">1.8.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_011042</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>[thioredoxin]-dithiol + NADP(+) = [thioredoxin]-disulfide + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20345"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.8.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="9">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.9" evidence="8"/>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9695873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DSZ3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004791">
        <property type="term" value="F:thioredoxin-disulfide reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019430">
        <property type="term" value="P:removal of superoxide radicals"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02949">
        <property type="entry name" value="TRX_NTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000064">
        <property type="entry name" value="Thioredoxin reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050097">
        <property type="entry name" value="Ferredoxin-NADP_redctase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008255">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_2_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005982">
        <property type="entry name" value="Thioredox_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01292">
        <property type="entry name" value="TRX_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48105">
        <property type="entry name" value="THIOREDOXIN REDUCTASE 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00469">
        <property type="entry name" value="PNDRDTASEII"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00573">
        <property type="entry name" value="PYRIDINE_REDOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 8" id="KW-0274">FAD</keyword>
    <keyword evidence="2 8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4 9" id="KW-0521">NADP</keyword>
    <keyword evidence="5 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7 8" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="11">
        <location>
            <begin position="407"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="71"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9695873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="533" mass="58357" checksum="052C593E93FACB65" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DUL3</accession>
    <name>A0AA39DUL3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CST complex subunit STN1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Suppressor of cdc thirteen homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_008404</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DUL3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040260">
        <property type="entry name" value="RFA2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13989:SF33">
        <property type="entry name" value="CST COMPLEX SUBUNIT STN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13989">
        <property type="entry name" value="REPLICATION PROTEIN A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0779">Telomere</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9696145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="138" mass="15288" checksum="2151354612B4EA12" modified="2024-01-24" version="1">MSNQLYNTHVKLLAFDLLSLTQTPSPSSSDPISFSRRGTPLSRAETLGTVTSRELKPHKFLKFTVDDGTGCVPCVLWLNHLSSPYFSRRNPADVRLIADLAEYQASEVKLGVLARVRGRITAYRECLVMEACSKANSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DYE6</accession>
    <name>A0AA39DYE6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Aminotransferase-like plant mobile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_006694</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DYE6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34835">
        <property type="entry name" value="OS07G0283600 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34835:SF34">
        <property type="entry name" value="OS08G0555500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="244"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="306"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="319"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9701453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="474" mass="53744" checksum="3A2BE801620B8918" modified="2024-01-24" version="1">MTNSMQSTRLYTRCSPSRFLKLCNRLPAEKLDVVRDLQFGGLLHLNCKEIRHNICIWLIAHFNVGFRRIDITSHKRYDLTAADVGLVFGLPTTGRILHIATTPSDHPFGTLNTCEERLLNLPIGEEFRRCFIYYTCATLLGPTSRIDGCRNLWHTIHEDGFRNDVNWGQFVVDQLVEGIRRYKQGNSVWVHGCILFLQLHYVIKFKIPSVHVPMTAPLLSAWSDELIKERLSAEISEFGSFGHGEGFDESSPARTHVEDDSGPTSSHEILDKYYAAERAINSYQKGIQQQLGIMRGLIHRLDARREKSVHSPTATHSAHAAEDFPEAEHDSPYARYDMPFHGTDEVPDTPIRHPPIIADDEVVVCDPLPLRSMTMARSHRSGRQRRVRRMPPNLLSPFIAQAQTRDSAIKMDLKEAAATVFDGDLDPSEELVSMHDTSLTRGNLASFQGDYWIGNDVNIPDCPLFCIILIFNML</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DZ81</accession>
    <name>A0AA39DZ81_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Isoflavone 2'-hydroxylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_006707</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DZ81"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20653">
        <property type="entry name" value="CYP81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000023">
        <property type="entry name" value="Cytochrome P450 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050651">
        <property type="entry name" value="Plant_Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47947">
        <property type="entry name" value="CYTOCHROME P450 82C3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47947:SF62">
        <property type="entry name" value="CYTOCHROME P450, FAMILY 81, SUBFAMILY D, POLYPEPTIDE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11 12" id="KW-0349">Heme</keyword>
    <keyword evidence="8 11 12" id="KW-0408">Iron</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 12" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="11">
        <location>
            <position position="440"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9701469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="508" mass="57286" checksum="B6343CE72A203F99" modified="2024-01-24" version="1">MEVSSLHSSLSLLFVFLLLVIGILFPRRRYGNVPPSPPAVPIIGHLHLLKQPAHRSLQRLSLKYGPIFSLRFGSQLVVIVSSPSAVEECFTKNDVVLANRPRWAPGKYVGFNYTSMAFAPYGEHWRNLRRLSALEIFSSNRLNMFLGIRRDEVKLLLLRLARDSREGFAKVELRPMLTELTFNIIMRMVAGKRYYGEGVEFEEAKRFRKIISEVFKLSGASSNPTDFLPILRWIGFGDHEKKLKQTMREIKVILQGLIDEHRSGNDRGSVDNNSMIDHLLSLQKTEPEYYTDDIIKGLVLVLILAGTDTSAATMEWAMTLLLNHPDVLKKAKAELDIHVGKDRLIEESDLPKLRYLQSIISETLRVFPVAPLLVPHMSSDDCQVGGFDIPGGTLLLINAWAIHRDPQVWEDPTSFIPERFENGERENYKLIPFGTGRRACPGVGLAHRVVGLALGSLIQCYDWKKISKTSIDTTEGKGLTMPKLEPLEAMCKACEIIKTGSLELENNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E2B0</accession>
    <name>A0AA39E2B0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">1-deoxy-D-xylulose-5-phosphate synthase</fullName>
            <ecNumber evidence="6">2.2.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_004305</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>D-glyceraldehyde 3-phosphate + pyruvate + H(+) = 1-deoxy-D-xylulose 5-phosphate + CO2</text>
            <dbReference type="Rhea" id="RHEA:12605"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57792"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="2.2.1.7"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:12606"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the transketolase family. DXPS subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.7" evidence="6"/>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E2B0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008661">
        <property type="term" value="F:1-deoxy-D-xylulose-5-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019288">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02007">
        <property type="entry name" value="TPP_DXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07033">
        <property type="entry name" value="TPP_PYR_DXS_TK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.920:FF:000002">
        <property type="entry name" value="1-deoxy-D-xylulose-5-phosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000005">
        <property type="entry name" value="1-deoxy-D-xylulose-5-phosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00315">
        <property type="entry name" value="DXP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005477">
        <property type="entry name" value="Dxylulose-5-P_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00204">
        <property type="entry name" value="dxs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003933">
        <property type="entry name" value="PRK05444.2-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43322">
        <property type="entry name" value="1-D-DEOXYXYLULOSE 5-PHOSPHATE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43322:SF3">
        <property type="entry name" value="1-DEOXY-D-XYLULOSE-5-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13292">
        <property type="entry name" value="DXP_synthase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="11" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="14">
        <location>
            <begin position="365"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00050872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9702502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="685" mass="75036" checksum="15AE71BDE4047E26" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E450</accession>
    <name>A0AA39E450_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">NAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_001767</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E450"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.150.80:FF:000002">
        <property type="entry name" value="Nac domain-containing protein 86"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.80">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003441">
        <property type="entry name" value="NAC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036093">
        <property type="entry name" value="NAC_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31744:SF216">
        <property type="entry name" value="NAC TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31744">
        <property type="entry name" value="PROTEIN CUP-SHAPED COTYLEDON 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02365">
        <property type="entry name" value="NAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101941">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51005">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="600"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="domain" description="NAC" evidence="13">
        <location>
            <begin position="9"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="164"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="354"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="519"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="177"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="369"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="532"/>
            <end position="553"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9706419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="631" mass="70389" checksum="30CD7D0675BED3DE" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E5H2</accession>
    <name>A0AA39E5H2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Increased DNA methylation 1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_002351</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E5H2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056511">
        <property type="entry name" value="IDM1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042163">
        <property type="entry name" value="PHF12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46309:SF5">
        <property type="entry name" value="GNAT FAMILY ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46309">
        <property type="entry name" value="PHD FINGER PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23209">
        <property type="entry name" value="IDM1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Increased DNA methylation 1 C-terminal" evidence="1">
        <location>
            <begin position="5"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF23209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9707320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="96" mass="10937" checksum="E383B146312813D4" modified="2024-01-24" version="1">MEGCFEPVIHTQINVVRSVVYNYGSNSPRISFEGFYKTILERQNEIISVAFVRIHGSNLAKCHFFATRPSYKCLGMCHKLLVAIESVSSLHINIFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E616</accession>
    <name>A0AA39E616_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_001619</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E616"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33978">
        <property type="entry name" value="SERINE/THREONINE-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33978:SF4">
        <property type="entry name" value="SERINE_THREONINE-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9706147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="153" mass="17147" checksum="5BE223C8110E1431" modified="2024-01-24" version="1">MEKPKEQKRDKSEEEAKSRIWDCDSSLYDSFELKSFKRQLDSAIVSSSRTFSMPHLADRGPPPPPQAAPSKKPSKFSRSFNKLLRSVFRSKPNPTSLFRVQEPFFFYDKSDALSTIPEASEIDYAGVSPDVNSLVKRSASDRFSASSLGISCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E6M3</accession>
    <name>A0AA39E6M3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Purple acid phosphatase</fullName>
            <ecNumber evidence="10">3.1.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_000585</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>a phosphate monoester + H2O = an alcohol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15017"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:67140"/>
            <dbReference type="EC" id="3.1.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="11">Binds 2 iron ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.2" evidence="10"/>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E6M3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07378">
        <property type="entry name" value="MPP_ACP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000027">
        <property type="entry name" value="Purple acid phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024927">
        <property type="entry name" value="Acid_PPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051558">
        <property type="entry name" value="Metallophosphoesterase_PAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10161:SF34">
        <property type="entry name" value="PURPLE ACID PHOSPHATASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10161">
        <property type="entry name" value="TARTRATE-RESISTANT ACID PHOSPHATASE TYPE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000898">
        <property type="entry name" value="Acid_Ptase_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 11" id="KW-0408">Iron</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 12" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041393738" description="Purple acid phosphatase" evidence="12">
        <location>
            <begin position="25"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="13">
        <location>
            <begin position="44"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="250"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR000898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000898-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9708624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="332" mass="37272" checksum="DECB638320F4EF77" modified="2024-01-24" version="1">MALLLSKAMAFYLVFTALVGLCSVSSVAELLGLEQPAKADGSLSFLVVGDWGRRGSFNQSQVALQMGRVGEELDIDFVVSTGDNFYDNGLSGVHDTAFEKSFSKVYTAPSLQKQWYNVLGNHDYRGKVEAQLSPILTKMDSRWLCLRSFILNAEIVEFFFVDTTPFVDKYFTDPEDHDYDWKGVLPRQAYLSNLLQDVDTALRDSTATWKIVVGHHTIRSAGHHGDTVELADQLLPILQANSVDLYINGHDHCLEHISSPDSPIQFLTSGGGSKAWRGDVQWWNPEELKFYYDGQGFMSVQITASQVDVAFYDVFGEVLHKWSKSKELYLPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E8A3</accession>
    <name>A0AA39E8A3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">glutathione transferase</fullName>
            <ecNumber evidence="1">2.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_000905</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the GST superfamily. Tau family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="1"/>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E8A3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009407">
        <property type="term" value="P:toxin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03185">
        <property type="entry name" value="GST_C_Tau"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03058">
        <property type="entry name" value="GST_N_Tau"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000044">
        <property type="entry name" value="Glutathione S-transferase GSTU6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000016">
        <property type="entry name" value="Glutathione S-transferase U9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045074">
        <property type="entry name" value="GST_C_Tau"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045073">
        <property type="entry name" value="Omega/Tau-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11260:SF615">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE U17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11260">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE, GST, SUPERFAMILY, GST DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01152">
        <property type="entry name" value="Main.3:_Omega-_and_Tau-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0216">Detoxification</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="6">
        <location>
            <begin position="4"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="7">
        <location>
            <begin position="89"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9709165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="233" mass="25882" checksum="568ECDE0BA738188" modified="2024-01-24" version="1">MATSDVKLLGAWPSPFALRVHIALNIKSVDYECIEENLRAKSQLLLQSNPVHKKVPVLIHHDKPICESLAIVQYIDEAWSSAPSILPSDPYDRAVARFWAAYIDDKWFPWLNGVANAQGAEAKKAALDQVREGLVVLEEAFGKCSEGKDFFSGDRIGYVDIALGCFLGWVYVAEKRNDVKLLLEDKMPGLAAWANKFCEDGAVKDVMPDKDELLEFANMVLDARAKLNASSNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E8L3</accession>
    <name>A0AA39E8L3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">EXS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_001030</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E8L3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000822">
        <property type="term" value="F:inositol hexakisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016036">
        <property type="term" value="P:cellular response to phosphate starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004342">
        <property type="entry name" value="EXS_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10783:SF104">
        <property type="entry name" value="PHOSPHATE TRANSPORTER PHO1 HOMOLOG 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10783">
        <property type="entry name" value="XENOTROPIC AND POLYTROPIC RETROVIRUS RECEPTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03124">
        <property type="entry name" value="EXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51380">
        <property type="entry name" value="EXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="EXS" evidence="6">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9709360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="104" mass="12048" checksum="9C300225B8B6217B" modified="2024-01-24" version="1">MQSKNYYLRDKLLVSHKSVYFAAMVLNILLRFAWLQSVLEFNACSLPKMTFSTMVSCLEILRRGIWSFFRYFALPEGTEVVALFLFHDHLKSQPVASLCDMTTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39E8Y0</accession>
    <name>A0AA39E8Y0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_001167</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39E8Y0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48017">
        <property type="entry name" value="OS05G0424000 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="185"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="272"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="311"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="8">
        <location>
            <begin position="83"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9709555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="337" mass="38049" checksum="D674EC973F220BA7" modified="2024-01-24" version="1">MPERGPLMIGFRSLSFRNAKWWYSAFHNVTAMVGAGDGRARMIVCFMSLNVTEYTKLSFHDDIFVSCDILGVLEWLFLFSHGSSRCTLWQMVEMHEMVPAKRFDRYHEVGQHAFREKLGLWIVVPQQVIVEVGVDIAYMITGGKSLQKFHNKVCPNCKPMRTSYFIMIFASCHFVLSHLPNFNSITGVLFAAATMSLTYSTIAWTALVHKGVQPDVQYTYTATTTTGRVFNFFSALGDVAFAYTGHNVVLEIQATIPSTPEKPSKEPMWKGVIFAYIVVALCYFPVALIGYWMFGNSVADNILVTLEKPRWLIAAANLFVAIHVIGSYQVQLQIFSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YHG1</accession>
    <name>A0AA38YHG1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">glucan endo-1,3-beta-D-glucosidase</fullName>
            <ecNumber evidence="4">3.2.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_026809</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;3)-beta-D-glucosidic linkages in (1-&gt;3)-beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.39"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the glycosyl hydrolase 17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.39" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YHG1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009506">
        <property type="term" value="C:plasmodesma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1040:FF:000001">
        <property type="entry name" value="Glucan endo-1,3-beta-glucosidase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000244">
        <property type="entry name" value="O-Glycosyl hydrolases family 17 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000490">
        <property type="entry name" value="Glyco_hydro_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012946">
        <property type="entry name" value="X8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044788">
        <property type="entry name" value="X8_dom_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31044">
        <property type="entry name" value="BETA-1,3 GLUCANASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31044:SF140">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00332">
        <property type="entry name" value="Glyco_hydro_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07983">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00768">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 15" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041380647" description="glucan endo-1,3-beta-D-glucosidase" evidence="15">
        <location>
            <begin position="26"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="X8" evidence="16">
        <location>
            <begin position="325"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9670502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="413" mass="44642" checksum="30177F0B54EE9BCB" modified="2024-01-24" version="1">MLKMGSLFHIIIFFLTFLVLTGSSSESVELLTLYDSSPVVLQALSHTGVPIAVSVREDDLNEVSGSVLMAESWIRTHVLAHYPSTKITTIVVGNTVLCNKDKEDKLRLVLPSLRNVYYSLTRWGLEKDIKVSAAFSSNCLNPDPVLDRDDMAEKVIKPLLSFLELTNSTYSVNPPPNFSPLSDESAGLVSSHSESMKKLGTSKLRVHVIVQSAKEGKPQSRKLSFMHSKFVEPYPARPTPLPELSPSPIHSSIGFTAPANVAKNPLPPLPPLEGTASPPPMIFPFAPEMPPIVIPASPPYSFSLPPCSPFEAGAPAPGTGVKQGLWCVAKPSVPADTLQEAIDYACGGGGADCDEIQPHGNCYYPDTVLAHASYAFNSYWQKHKKNGGTCSFGGTAMLINADPSFLHCRFLHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YHT0</accession>
    <name>A0AA38YHT0_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_026938</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-lysyl(27)-[histone H3] + 3 S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl(27)-[histone H3] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:60292"/>
            <dbReference type="Rhea" id="RHEA-COMP:15535"/>
            <dbReference type="Rhea" id="RHEA-COMP:15548"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="2.1.1.356"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YHT0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031519">
        <property type="term" value="C:PcG protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140951">
        <property type="term" value="F:histone H3K27 trimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd10519">
        <property type="entry name" value="SET_EZH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.270.10:FF:000001">
        <property type="entry name" value="Putative histone-lysine N-methyltransferase EZH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026489">
        <property type="entry name" value="CXC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045318">
        <property type="entry name" value="EZH1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025778">
        <property type="entry name" value="Hist-Lys_N-MeTrfase_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058609">
        <property type="entry name" value="HTH_CLF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041355">
        <property type="entry name" value="Pre-SET_CXC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033467">
        <property type="entry name" value="Tesmin/TSO1-like_CXC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45747">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE E(Z)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45747:SF4">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE E(Z)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25996">
        <property type="entry name" value="HTH_CLF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18264">
        <property type="entry name" value="preSET_CXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01114">
        <property type="entry name" value="CXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51633">
        <property type="entry name" value="CXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51576">
        <property type="entry name" value="SAM_MT43_EZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CXC" evidence="9">
        <location>
            <begin position="671"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="domain" description="SET" evidence="8">
        <location>
            <begin position="785"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="365"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="479"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="910"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="391"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="418"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="479"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="910"/>
            <end position="923"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9670710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="934" mass="105084" checksum="13DD81D5542FA750" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YHT5</accession>
    <name>A0AA38YHT5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HXXXD-type acyl-transferase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_026930</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9670697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YHT5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051283">
        <property type="entry name" value="Sec_Metabolite_Acyltrans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31896">
        <property type="entry name" value="FAMILY REGULATORY PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G14730)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31896:SF43">
        <property type="entry name" value="PROTEIN ENHANCED PSEUDOMONAS SUSCEPTIBILITY 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9670697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="451" mass="49731" checksum="06ADE9116CAD8A33" modified="2024-01-24" version="1">MEGIISFISTTAVRPASPEESTKRIELTPWDLQLLLLGYIKKGLLFLKPTPGPEENGLETMTAIVDHLKTSLSRALDFFYPLAGRLGTTVNDDDDTTCFFIDCNGAGAQFIHAAAGGVTVADILEPVYVPEIVSSFFPLDGVQNYHGVTQPLLAVQVTELVDGIFVGCTLNHVVGDGKSFWHFFNSWSEISRSPNKISVSPVFKRWFGDDTHYPIRIPQSAMVFDRPITSSPVQEMVFRSSKEKIAGLKARANAEMGTDRISSLQALLAHLWRCTIRTRRLPEDQETSYHLSIDMRTRRRPQMPQQYFGVAVQRGTLTMKVGELLGMGLGHVAWQMNKMISTFTEVEATNFLESWAKDPKPISYSTVPMSNALIMASSPRFNVYGTDFGWGRPVAVRSGGGIKFDGKTTISQGAEEGSIDIEVCSSPETLQGMLEDADFMGAVTASSNFFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YIP4</accession>
    <name>A0AA38YIP4_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_027261</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000020">
        <property type="protein sequence ID" value="KAJ9671191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YIP4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35118">
        <property type="entry name" value="KINASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35118:SF2">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9671191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="752" mass="84248" checksum="437B0F86E7B0AA68" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YJZ9</accession>
    <name>A0AA38YJZ9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PVL29_025501</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 20">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 20">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YJZ9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048544">
        <property type="term" value="P:recognition of pollen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01098">
        <property type="entry name" value="PAN_AP_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000195">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.4.10">
        <property type="entry name" value="Hepatocyte Growth Factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000858">
        <property type="entry name" value="S_locus_glycoprot_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32444">
        <property type="entry name" value="BULB-TYPE LECTIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32444:SF247">
        <property type="entry name" value="OS01G0958200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08276">
        <property type="entry name" value="PAN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00954">
        <property type="entry name" value="S_locus_glycop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00473">
        <property type="entry name" value="PAN_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4 14" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041377500" description="non-specific serine/threonine protein kinase" evidence="14">
        <location>
            <begin position="17"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="224"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="68"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="17">
        <location>
            <begin position="124"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="294"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ9671825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="427" mass="48489" checksum="AD0D2E7561A27232" modified="2024-01-24" version="1">MKIDSVVVLFLWRTLFKPTFVANKEEAYHAFEAMDSAIYSGIVILESGLVQHFSWIGDLQWAVLYGIQKDHCDAYNLCGPFGVCYIINQSPKCECMMGFTPNSPKDWEVFNTFGGCVWISPLECQRGNGFVKYPGMKLPDSSNLWVNVSMGTKECRAECLKNCSCTGYAEMDTHGKGKGCLMWFGPLIDIRQSTSFGQDLYVRMSASELESIADSQKRRRRLKLLLVLLAFFAVLISDLLLWYIAWKKKRAHGRDDKTIDNNERYLTDESLEDEEEGKFPLFDLTTIAAATKNFAFANKIGEGGFGPVYKAVLPTGEEIAVKKLSHTSRQGLKELKNEVNLISRIQHRNLVRLLGCCIEVEERMLIYEYMANRSLDAFIFGLAPQHFVHSPNLLFGCRGGKEKKIWSLITTIQTICCSFPRLIFSWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YK44</accession>
    <name>A0AA38YK44_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Beta-glucosidase 41</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_025541</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9671944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YK44"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000020">
        <property type="entry name" value="Beta-glucosidase 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033132">
        <property type="entry name" value="GH_1_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001360">
        <property type="entry name" value="Glyco_hydro_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353">
        <property type="entry name" value="GLYCOSYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353:SF36">
        <property type="entry name" value="LP05116P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00232">
        <property type="entry name" value="Glyco_hydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00131">
        <property type="entry name" value="GLHYDRLASE1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00653">
        <property type="entry name" value="GLYCOSYL_HYDROL_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041216435" description="Beta-glucosidase 41" evidence="5">
        <location>
            <begin position="20"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9671944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="504" mass="57942" checksum="D4C5C5A58B1AC279" modified="2024-01-24" version="1">MAVLLVLFLMSCLFMNSESISRVDFPDGFIFGTASSAYQFEGAVDEGNKGVSIWDTFTRQPGRILDFSNADMAVDQYHRFKTDIDLMKDLGMDAYRFSISWSRIFPNGTGEPNLEGIEYYNCLIDALLEKGIQPYVTLYHWDLPQMLEDRYEGWLSKQIVKDFEHYASTCFQAFGDRVKNWITFNEPHGFALQGYDTGLQAPGRCSILGHLFCKTGESSTEPYIVAHNILLSHAAVYHNYQLHFKECQGGLIGMALDAKWYEPISDSDEDKDAARRAMDFGIRWFLDPLFFGEYPQSMQRLVGKRLPEISPKTAKFLSGSLDFVGINHYTTLYVRNDRTQIRKFILRDASSDAAVITTSFRGGEAIGERAASRWLHIVPWGIRKLARYVKDNYGNPPVIITENGMDDPNRGSIPLEKALQDDKRINFHRDYLTNLSAAIRQDNCDVRGYFVWSLLDNWEWNLGYSVRFGLYFVDYKNNLTRIPKTSAQWFRRILRSSSGLRNQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YLB7</accession>
    <name>A0AA38YLB7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PRA1 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_025976</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">May be involved in both secretory and endocytic intracellular trafficking in the endosomal/prevacuolar compartments.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the PRA1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YLB7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004895">
        <property type="entry name" value="Prenylated_rab_accept_PRA1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19317:SF53">
        <property type="entry name" value="PRA1 FAMILY PROTEIN G1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19317">
        <property type="entry name" value="PRENYLATED RAB ACCEPTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03208">
        <property type="entry name" value="PRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="67"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="119"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="145"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9672571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="180" mass="19826" checksum="A53FAEBFE7FD43F9" modified="2024-01-24" version="1">MSTPTATPATYTSIPISGADVISRSFHNLSAAVSSRRPWPEFVAADVFHRPDSLIVAGERVRKNASYFRINYGILILGCAMISLVGTPISLMVWVLTLGMWLIFYFFREDPLFVGGRQVSDRLILLCLVLASVMAVWFTGVLQNLVVGVGIGLLVCCVHGVFRNPLEDDEARLRESALTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YLE5</accession>
    <name>A0AA38YLE5_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_025978</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000019">
        <property type="protein sequence ID" value="KAJ9672575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YLE5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040276">
        <property type="entry name" value="At4g26450-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36056">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36056:SF1">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="127"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="346"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="434"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="250"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="346"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9672575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="729" mass="79279" checksum="CE9E14C9A8DA72B5" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YMM8</accession>
    <name>A0AA38YMM8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">UVR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_023029</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YMM8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38394">
        <property type="entry name" value="NEUROFILAMENT LIGHT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38394:SF1">
        <property type="entry name" value="NEUROFILAMENT LIGHT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="22"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="69"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="120"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="239"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="404"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="530"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="689"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="42"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="73"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="127"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9673169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="753" mass="84232" checksum="3A3834DA1CBB445A" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YNM9</accession>
    <name>A0AA38YNM9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GrpE protein homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_023343</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the GrpE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9673727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YNM9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000774">
        <property type="term" value="F:adenyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00446">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.20.20:FF:000006">
        <property type="entry name" value="GrpE protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.22.10:FF:000001">
        <property type="entry name" value="Protein GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.22.10">
        <property type="entry name" value="Head domain of nucleotide exchange factor GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01151">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000740">
        <property type="entry name" value="GrpE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013805">
        <property type="entry name" value="GrpE_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009012">
        <property type="entry name" value="GrpE_head"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010738">
        <property type="entry name" value="PRK14140.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010741">
        <property type="entry name" value="PRK14143.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21237:SF40">
        <property type="entry name" value="CELL CYCLE AND APOPTOSIS REGULATOR PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21237">
        <property type="entry name" value="GRPE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01025">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00773">
        <property type="entry name" value="GRPEPROTEIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58014">
        <property type="entry name" value="Coiled-coil domain of nucleotide exchange factor GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51064">
        <property type="entry name" value="Head domain of nucleotide exchange factor GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01071">
        <property type="entry name" value="GRPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0346">Stress response</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="268"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="104"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9673727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="298" mass="33469" checksum="6237C1D1DEC1A721" modified="2024-01-24" version="1">MAFSLSYHLLFTPKPSKTLALHPPHPSKPILGFRLSTSSRILRRSFKPFLALQDSTPYTNNEEEGSRYDVKTSKSEADQTPLSVLKSLIKAYKDAVLDGDEKAVSEIEAMIDTIESEKTELAQKVSALSAEITSGKEKYIRLQADFDNFRRRSEKERLTVRTNAQGEVVESLFPMIDNFERAKQQIKPETEKEKKIDTSYQGIYKQFVEIMRSFHVAAVATVGKPFDPALHEAIAREESQEFKEGIIIQEFRRGFLLGDRLLRPAMVKVSTGPGRKKTSAVADKSKGQPATAAGVDER</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YPT4</accession>
    <name>A0AA38YPT4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_023777</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YPT4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="28"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9674433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="105" mass="12143" checksum="97B8044D03E40058" modified="2024-01-24" version="1">MPRHWNPFPSPVEDKTIPTVEGKKSVPYTKQQLHEFFKEHDKNGDKFLSKEELTKAFNDLQSRNPGFRAWRAMGKADADGDQHISQQEMEALLDYAVGIKYHIKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YSC5</accession>
    <name>A0AA38YSC5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="4">3.4.19.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Deubiquitinating enzyme MINDY-3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_024486</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Hydrolase that can remove 'Lys-48'-linked conjugated ubiquitin from proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MINDY deubiquitinase family. FAM188 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YSC5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990380">
        <property type="term" value="F:K48-linked deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071108">
        <property type="term" value="P:protein K48-linked deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025257">
        <property type="entry name" value="MINDY-3/4_CD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039785">
        <property type="entry name" value="MINY3/4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12473">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE MINDY-4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12473:SF8">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE MINDY-4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13898">
        <property type="entry name" value="MINDY-3_4_CD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01174">
        <property type="entry name" value="DUF4205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50330">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Deubiquitinating enzyme MINDY-3/4 conserved" evidence="8">
        <location>
            <begin position="115"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="68"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00033208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9675584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="616" mass="66983" checksum="8C384273F35FC8A2" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YSM8</accession>
    <name>A0AA38YSM8_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_024589</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9675734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YSM8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9675734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="70" mass="7844" checksum="882AA1DBF71BBC0D" modified="2024-01-24" version="1">MDRVGKQGEDELKVEVELLSRFGSPYLLTLLGSWANLTLGPPPMSLYAKFMKRGLNLGGPVWFFSDILKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YST6</accession>
    <name>A0AA38YST6_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_024825</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9676026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YST6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9676026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="79" mass="9067" checksum="F0A8C34A10C621D5" modified="2024-01-24" version="1">MKNLITCSKALSLSINTSESEFGDVPNRFIAMRPPYTWRTQLYQTSLSKDVTDSQMPLSCGLRANKEIDFPDCTRTFWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YTV2</accession>
    <name>A0AA38YTV2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S ribosomal protein L7a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_021878</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9676578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YTV2"/>
    <dbReference type="SMR" id="A0AA38YTV2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000003">
        <property type="entry name" value="60S ribosomal protein L7a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050257">
        <property type="entry name" value="eL8/uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004037">
        <property type="entry name" value="Ribosomal_eL8-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018492">
        <property type="entry name" value="Ribosomal_eL8/Nhp2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001921">
        <property type="entry name" value="Ribosomal_eL8_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23105">
        <property type="entry name" value="RIBOSOMAL PROTEIN L7AE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00881">
        <property type="entry name" value="L7ARS6FAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00882">
        <property type="entry name" value="RIBOSOMALL7A"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01082">
        <property type="entry name" value="RIBOSOMAL_L7AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="5">
        <location>
            <begin position="117"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9676578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="258" mass="29387" checksum="3DF7BC1BA0D89786" modified="2024-01-24" version="1">MAPKKGGKVLAQTKKKPEKVVNPLFEKRPKQFGIGGALPPKRDLHRFVKWPHVVRIQRKRRILKQRLKVPPALNQFTKTLDKNLATSLFKMLLKYRPEDKAAKKERLLKRAQSEAEGKTIEVKKPIVVKYGLNHVTYLIEQNKAQLVVIAHDVDPIELVVWLPALCRKMEIPYCIVKGKSRLGAIVHKKTAAVLCLTTVKNEDKLEFSKILEAIKANFNDKYDENRKKWGGGIMGSKSQAKTKAKEKLLAKEAAQRMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YTW6</accession>
    <name>A0AA38YTW6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pyruvate dehydrogenase E1 component subunit beta</fullName>
            <ecNumber evidence="5">1.2.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PVL29_021857</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 5">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[protein] + pyruvate + H(+) = N(6)-[(R)-S(8)-acetyldihydrolipoyl]-L-lysyl-[protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:19189"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10478"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83111"/>
            <dbReference type="EC" id="1.2.4.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.4.1" evidence="5"/>
    <dbReference type="EMBL" id="JARBHA010000017">
        <property type="protein sequence ID" value="KAJ9676549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YTW6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004739">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006086">
        <property type="term" value="P:pyruvate decarboxylation to acetyl-CoA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07036">
        <property type="entry name" value="TPP_PYR_E1-PDHc-beta_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.920:FF:000001">
        <property type="entry name" value="Pyruvate dehydrogenase E1 beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027110">
        <property type="entry name" value="PDHB_mito-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11624">
        <property type="entry name" value="DEHYDROGENASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11624:SF112">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT SUBUNIT BETA-1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4 5" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="6">
        <location>
            <begin position="70"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ9676549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="334" mass="36160" checksum="A57A7C90B0DECA11" modified="2024-01-24" version="1">MFKSILIDGNGTTSFHAGHLSRCLGFRIIFRDPCVPLLHDDTHYQPDQALNRIRPVVYASRSYASGPKQMTVREALNTAIDEEMSADPKVFLMGEEVGEYQGAYKISKGLLDKYGPGRVIDTPITEAGFAGIGVGAAYHGLKPIVEFMTFNFSLQAIDHIINSAAKSNYMSAGQISVPIVFRGPNGAAAGVGAQHSQIEREGKDVTIVTYARMVDYSLQAAEILAKEGISAEVINLRSIRPLDRSAINASVRKTSRLVTVEEGFPQHGVGAEICMSVIEESFDSLDAPVERIAGADIPMPYAANLERMALPQIDDILRAAKRTCYRSAPKAAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YZK4</accession>
    <name>A0AA38YZK4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">CNNM transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_021461</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YZK4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030026">
        <property type="term" value="P:intracellular manganese ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010960">
        <property type="term" value="P:magnesium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04590">
        <property type="entry name" value="CBS_pair_CorC_HlyC_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.580.10:FF:000015">
        <property type="entry name" value="DUF21 domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045095">
        <property type="entry name" value="ACDP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002550">
        <property type="entry name" value="CNNM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044751">
        <property type="entry name" value="Ion_transp-like_CBS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12064:SF36">
        <property type="entry name" value="DOMAIN-CONTAINING PROTEIN, PUTATIVE, EXPRESSED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12064">
        <property type="entry name" value="METAL TRANSPORTER CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01595">
        <property type="entry name" value="CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51846">
        <property type="entry name" value="CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8" id="KW-0129">CBS domain</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="13"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="98"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="CNNM transmembrane" evidence="13">
        <location>
            <begin position="9"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="12">
        <location>
            <begin position="211"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="374"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="501"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="530"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9679549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="540" mass="59215" checksum="9D48C46B42F8DABA" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38YZK9</accession>
    <name>A0AA38YZK9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ACT domain-containing protein ACR</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Protein ACT DOMAIN REPEATS</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_021350</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Binds amino acids.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38YZK9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040217">
        <property type="entry name" value="ACR1-12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31096">
        <property type="entry name" value="ACT DOMAIN-CONTAINING PROTEIN ACR4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31096:SF55">
        <property type="entry name" value="ACT DOMAIN-CONTAINING PROTEIN ACR6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01842">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ACT" evidence="3">
        <location>
            <begin position="75"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU369043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9679393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="302" mass="33819" checksum="ED09489821BCB6E2" modified="2024-01-24" version="1">MSSANPPIVVIDNDASEEATVIQVESVNKHGILLEVVQVLTDMNLVITKTLESDASFVPSMRGTVGVMPSEDHTSIELSGTDRPGLLSKVCAVLVDLHWNVVNAEIWTHNARVAAVHVTDDSTGCAIEDPNQLSKIKELLCNVLKGNNDLKTAKMTFTPWIMFADRDYQRVERAELGKDKSSRPRVTVLDCIEKDYTVITMRSKDRPKLLFDTICTLTDMQYVVFHGVVHTGRMEAYQVRHVDGLPMSSDAERERVIQCLEAAIERRASEGLELELCTEDRFGRLSDITRVFRENSLCIERA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z0F8</accession>
    <name>A0AA38Z0F8_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_019385</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z0F8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052751">
        <property type="entry name" value="Plant_MAPKKK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48011">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTIONAL COMPLEX SUBUNIT CAF120-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48011:SF7">
        <property type="entry name" value="F10K1.14 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9680085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="144" mass="16565" checksum="1D7D905504FC6ACB" modified="2024-01-24" version="1">MFFDELPKFPAQLSDSGCNFLEKCLRREPTERWSCDQLLQHPFISSSSPNYITEASSRSVLDCFSKADLKSAEKRRSCHVEKSRVELEELEGIGAKSTNAVWFQREKRLNCYCLNKYKAHNSDSLIRSMGESALTMEILVVNKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z1G7</accession>
    <name>A0AA38Z1G7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phytocyanin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_019763</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z1G7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04216">
        <property type="entry name" value="Phytocyanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000003">
        <property type="entry name" value="Blue copper"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039391">
        <property type="entry name" value="Phytocyanin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003245">
        <property type="entry name" value="Phytocyanin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33021">
        <property type="entry name" value="BLUE COPPER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33021:SF350">
        <property type="entry name" value="UCLACYANIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02298">
        <property type="entry name" value="Cu_bind_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51485">
        <property type="entry name" value="PHYTOCYANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0186">Copper</keyword>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041376287" description="Phytocyanin domain-containing protein" evidence="6">
        <location>
            <begin position="20"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="156"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="Phytocyanin" evidence="7">
        <location>
            <begin position="20"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="117"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="118"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9680539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="175" mass="17928" checksum="380F7B0F504F342F" modified="2024-01-24" version="1">MAMAAALLLLLLAAPVIYATNHTVGGSSGWDTGVNYTAWASGQTFTVGDYLVFTYGSTHSVDQVTQTNYDNCGTSNPTKSYSGGSNTIALTTSGSMYFLCPTIGHCSQGMKLAISVVSNSGSTSPPSNSSSPSPPPPPRNSSSSSPSGATSKFCSMSHLMVGLVLGFGMMFALVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z282</accession>
    <name>A0AA38Z282_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_020101</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9681033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z282"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050667">
        <property type="entry name" value="PPR-containing_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47939">
        <property type="entry name" value="MEMBRANE-ASSOCIATED SALT-INDUCIBLE PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47939:SF13">
        <property type="entry name" value="OS03G0201400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12854">
        <property type="entry name" value="PPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="44"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="79"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="150"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="224"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="295"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="330"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="402"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="437"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9681033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="503" mass="56314" checksum="0181096575F94A3F" modified="2024-01-24" version="1">MSVKWPRFLIPTQLSQMIRTQKNPLTALKIFNEAKSKFPNYRHNGPVYATMINVLGSSGRIAEMREVIDQMKQDSCECKDSVFSSVLKTYARAGMVDEAVSLFKTLPQFNCVNWTGSFNTLLRILVKESKLETACRLFLEHSCGWEVKSRIGSLNLLMDALCQINRSDLALHVFQEMRYQCCSPDKESYRILMRGLCADGRLNEATHLLYSMFWRISQKGSGEDIAVYRTLLDALCDNGHVEEALEILGKVLKKGLKAPKRCRGHLDLSYCRNGEDIERTKGLINEALIRGGVPSMASYSAMAIDLYSERKIGEANQVLDEMRDRGFKPSLSIYEAKIAALCRCGGVEEAVKVIEEEMVEDSCVPTVRLYNILLKGLCQEGKSAFAVGYLEKMARQVGCVADKETYSILVDGLCCDGRFVEASGILERMLIRSSSPGVETYNTLIRGLCLMGRQYDAVIWLEEMVVQGKLPAVSVWDSLVASVCSNMTEAVVCSETLEKLWSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z3F0</accession>
    <name>A0AA38Z3F0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">RNA polymerase II subunit B1 CTD phosphatase RPAP2 homolog</fullName>
            <ecNumber evidence="12">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PVL29_017862</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Putative RNA polymerase II subunit B1 C-terminal domain (CTD) phosphatase involved in RNA polymerase II transcription regulation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 12">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 12">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11 12">Belongs to the RPAP2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="12"/>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9681676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z3F0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043175">
        <property type="term" value="F:RNA polymerase core enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008420">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.820:FF:000006">
        <property type="entry name" value="Putative RNA polymerase II subunit B1 CTD phosphatase RPAP2 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039693">
        <property type="entry name" value="Rtr1/RPAP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007308">
        <property type="entry name" value="Rtr1/RPAP2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038534">
        <property type="entry name" value="Rtr1/RPAP2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14732">
        <property type="entry name" value="RNA POLYMERASE II SUBUNIT B1 CTD PHOSPHATASE RPAP2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14732:SF0">
        <property type="entry name" value="RNA POLYMERASE II SUBUNIT B1 CTD PHOSPHATASE RPAP2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04181">
        <property type="entry name" value="RPAP2_Rtr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51479">
        <property type="entry name" value="ZF_RTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7 12" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RTR1-type" evidence="14">
        <location>
            <begin position="32"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="179"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="231"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="236"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="275"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="326"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU367080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ9681676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="659" mass="72484" checksum="131F522B96FF4A28" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z412</accession>
    <name>A0AA38Z412_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glycosyl transferase CAP10 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_020412</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9681524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z412"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006598">
        <property type="entry name" value="CAP10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051091">
        <property type="entry name" value="O-Glucosyltr/Glycosyltrsf_90"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12203:SF80">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12203">
        <property type="entry name" value="KDEL LYS-ASP-GLU-LEU CONTAINING - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05686">
        <property type="entry name" value="Glyco_transf_90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00672">
        <property type="entry name" value="CAP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase CAP10" evidence="2">
        <location>
            <begin position="187"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9681524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="516" mass="59985" checksum="B0FF4CF3DB3D6CD3" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z5X3</accession>
    <name>A0AA38Z5X3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PVL29_018549</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z5X3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000307">
        <property type="entry name" value="pollen receptor-like kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000585">
        <property type="entry name" value="Probable inactive receptor kinase At1g48480"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000234">
        <property type="entry name" value="Probable inactive receptor kinase RLK902"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050994">
        <property type="entry name" value="At_inactive_RLKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48010">
        <property type="entry name" value="OS05G0588300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48010:SF32">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 15" id="KW-0732">Signal</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041437089" description="Protein kinase domain-containing protein" evidence="15">
        <location>
            <begin position="24"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="233"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="334"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="263"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="271"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="365"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ9682647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="639" mass="69181" checksum="A17FED9C25A33C70" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z8J5</accession>
    <name>A0AA38Z8J5_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_016486</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z8J5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044550">
        <property type="term" value="P:secondary metabolite biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47944">
        <property type="entry name" value="CYTOCHROME P450 98A9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47944:SF4">
        <property type="entry name" value="OS09G0441700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0349">Heme</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9684012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="269" mass="30743" checksum="72CE4444992C4C37" modified="2024-01-24" version="1">MWLLQKRIEEADNLVRFIYNQCKSSTTTDNFMDSSVVNVRNAVRQYTGNIVTKMMFSRSYFGEGRKGGGHGLEEEEHVNSLFTSLAYLHAFSPSDYLSCLRVFDLDGHQKMVKEALSIINKHHDPIVDERMIQWRNGEKREVEDILEVFITIRDSKGEPLLSELMIEIVDNPAQAAEWAMAEMINQPEIMQKAVEEIDRVVGKERLVQESDIMQEALRFHPMAPFNVPHLSMADAVVASYFIPKGSHVLLSRVGLGRNPIKVQTRAAYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z9C0</accession>
    <name>A0AA38Z9C0_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_016902</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z9C0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9684662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="74" mass="8153" checksum="0FF6E2AFED6378AA" modified="2024-01-24" version="1">MHGGLEVSDENGAISDLDIVMALVGNKANLHENREVPVQRTNGMFFIETSAKTVDNINQLFEEIAKRLPRPSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38Z9Y5</accession>
    <name>A0AA38Z9Y5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Replication protein A 14 kDa subunit B-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_017104</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the replication factor A protein 3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38Z9Y5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031981">
        <property type="term" value="C:nuclear lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04479">
        <property type="entry name" value="RPA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013970">
        <property type="entry name" value="Rfa2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47058">
        <property type="entry name" value="REPLICATION PROTEIN A 14 KDA SUBUNIT A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47058:SF3">
        <property type="entry name" value="REPLICATION PROTEIN A 14 KDA SUBUNIT A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08661">
        <property type="entry name" value="Rep_fac-A_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9684950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="107" mass="11654" checksum="D7E9E370C7C33D6A" modified="2024-01-24" version="1">MDTSSPAVFVNAELLRTYVGRKVRAVVQVIGFDGGVVTGKSVDDQQLIIKGSPPSVPVMNYIEVIGIADSNQSIRAELWTNFGSTFDTHSYNQLCLLANGEFKSLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZAI9</accession>
    <name>A0AA38ZAI9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_017149</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZAI9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052343">
        <property type="entry name" value="Retrotransposon-Effector_Assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46890">
        <property type="entry name" value="NON-LTR RETROLELEMENT REVERSE TRANSCRIPTASE-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46890:SF48">
        <property type="entry name" value="RNA-DIRECTED DNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9685003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="75" mass="8228" checksum="900992703AF56FE6" modified="2024-01-24" version="1">MGFGAKWMDWMWSCSSTAKFSVLVNGEPAGFFSSTKGLRQGDPLSPYLFVMGMEVLSVLIRRAVEGGFISGCKIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZAL2</accession>
    <name>A0AA38ZAL2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein YIP</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_017079</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Golgi apparatus membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the YIP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZAL2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048280">
        <property type="term" value="P:vesicle fusion with Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045231">
        <property type="entry name" value="Yip1/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006977">
        <property type="entry name" value="Yip1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21236">
        <property type="entry name" value="GOLGI MEMBRANE PROTEIN YIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21236:SF2">
        <property type="entry name" value="PROTEIN YIPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04893">
        <property type="entry name" value="Yip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="137"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="167"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="196"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="230"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="Yip1" evidence="7">
        <location>
            <begin position="109"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9684914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="248" mass="26991" checksum="E1D7EE77503C7B1C" modified="2024-01-24" version="1">MSKEFNVPPVVFPAGGNPGPAAVAQRRLPTAPFQPPRPANPSIPFMSFDVGSAAAATSFPAPQFGSNTIGIGSNFDDEPPLLEELGINTKQIWNKTISILNPFRVNPNLHEDADLSGPFLFLMSFGLFQLLAGKIHFGIILGWVIVAALFLYVVFNMLAGRNGNLDLYRCLSLIGYCMLPLVILSAFSLFVPQGGLVIFGMAAVFVLWSTRVCTRLLVELASCGDEHRGLITYACFLIYMLFSLLVIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZAL7</accession>
    <name>A0AA38ZAL7_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_017387</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZAL7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34271">
        <property type="entry name" value="NUCLEOLAR HISTONE METHYLTRANSFERASE-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34271:SF1">
        <property type="entry name" value="NUCLEOLAR HISTONE METHYLTRANSFERASE-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9685337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="178" mass="19946" checksum="1E5B5153E603B752" modified="2024-01-24" version="1">MPPRAFAVKEISCNLAVIFLILDHISALNLIAFGVKLVQQEGMRLLALNLDGTYNISDDNDTSSAAMPSTGVEDATSHTNEIGNEDCNPLAEEEITNERDISLDQNPFHSLPHLISSSTLIDSLLLMQRHMRFCGWINDNEPNDICTKQLIPTTVMQLEKVIRETDTHSKSRWDEKPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZEQ2</accession>
    <name>A0AA38ZEQ2_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_015914</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZEQ2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9687237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="47" mass="4817" checksum="CB73E9194A8BB189" modified="2024-01-24" version="1">MGRVEEDVTIAMAVVGASAGGEILPGQCTAKRVVACGGRQKQGMRKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZFJ6</accession>
    <name>A0AA38ZFJ6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Disease resistance protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_016248</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZFJ6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057135">
        <property type="entry name" value="At4g27190-like_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050905">
        <property type="entry name" value="Plant_NBS-LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463:SF209">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RPS2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23247">
        <property type="entry name" value="LRR_RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NB-ARC" evidence="8">
        <location>
            <begin position="136"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="10">
        <location>
            <begin position="393"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="11">
        <location>
            <begin position="499"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein At4g27190-like leucine-rich repeats" evidence="9">
        <location>
            <begin position="795"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="30"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9687687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="958" mass="109467" checksum="A75516F8586AF94C" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZGH5</accession>
    <name>A0AA38ZGH5_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_014345</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9688641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZGH5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34569:SF18">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34569">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9688641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="227" mass="25222" checksum="2C0C0F486B2DD5AD" modified="2024-01-24" version="1">MASLTPVSQSQSPLNKPQFKSPTLQASFLPVSLMGPSFRFRVLRRHGSVPARRGGALEMATSIDGYLRFHVDSKLVYDTLEGIVQKAAFDSRESLICKCSLHLIICFRALEFIENYDWFCIHNLTSLKDLTPSSPTISLAPCTIQSAYQIPISNLLVKQAAWAYLQPMPVLASSNKHFFPSLPPPTRACVVFFTTITRALNRVLGAIRGCEFAGTWENNHRYAVGVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZGH6</accession>
    <name>A0AA38ZGH6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PVL29_013959</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the RING-type zinc finger family. ATL subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9687988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZGH6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16461">
        <property type="entry name" value="RING-H2_EL5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000609">
        <property type="entry name" value="RING-H2 finger protein ATL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044600">
        <property type="entry name" value="ATL1/ATL16-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46913">
        <property type="entry name" value="RING-H2 FINGER PROTEIN ATL16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46913:SF1">
        <property type="entry name" value="RING-H2 FINGER PROTEIN ATL16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="26"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="17">
        <location>
            <begin position="103"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="156"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="158"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ9687988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="187" mass="21217" checksum="6CF5463410C0C98B" modified="2024-01-24" version="1">MFEIPSPFLPPSPPPLPPPVKHQLPMFYYGLIVVGTAAFVLALYNLIVIRWCSNNRRRSQRREHPFFDPRTSQSFENMNLGLVSSFKYRKEGVAQEQAAEYECAVCLSVFEEGEDVRKLPKCKHSFHAPCIDMWLYSHSDCPLCRARVEPAVVHRQDQAQAQAEAETEAVTQPENPRGGLRDSGESV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZI57</accession>
    <name>A0AA38ZI57_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Spermidine hydroxycinnamoyl transferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_014765</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9689254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZI57"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000008">
        <property type="entry name" value="Tryptamine hydroxycinnamoyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050317">
        <property type="entry name" value="Plant_Fungal_Acyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31642:SF324">
        <property type="entry name" value="SPERMIDINE HYDROXYCINNAMOYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31642">
        <property type="entry name" value="TRICHOTHECENE 3-O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9689254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="456" mass="50291" checksum="ED580945FDD40429" modified="2024-01-24" version="1">MVVVSLKASYTVKPSEPTFDGRLSLSEWDQIGTITHVPTIYFYRPSQDWILRDGDILATLRDSLSRVLVHFYPLAGRLCWIQGSRLELECNAMGVQLIEAESQAKLDDFGDFAPSPDLEYLVPCVNYNSPIHELPLMLAQLTKFSCGGISLATSISHAVADGQSALHFIDEWARLARGEPLGTPPFLDRKLLRAGDSPIAPPCFDHAEFNHPPLLMGASSTVEERKKGTTVSLLKLTKTQVERLKKMANDSRPTECGRPYSRYEAIAAHIWRCACNARGHKPEQPTAIGICIDSRSRMRPPLPQGYFGNATLDVIATGRSGELMSKPLGYASSRIRAAIEKVTDEYVWSAIDFLKNQPDLSLFQDLHALGSDEGPFYGNPNLGVISWLTLPMYGVDFGWGKEVYMGPGPHDFDGDSLILPGHDGDGSLVVALCLQVAHMDAFKNFFYEAIAESSIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZJE7</accession>
    <name>A0AA38ZJE7_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_012691</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZJE7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010333">
        <property type="term" value="F:terpene synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.130">
        <property type="entry name" value="Terpene synthase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001906">
        <property type="entry name" value="Terpene_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036965">
        <property type="entry name" value="Terpene_synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050148">
        <property type="entry name" value="Terpene_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005630">
        <property type="entry name" value="Terpene_synthase_metal-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225">
        <property type="entry name" value="OS04G0344100 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225:SF234">
        <property type="entry name" value="TERPENE SYNTHASE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01397">
        <property type="entry name" value="Terpene_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03936">
        <property type="entry name" value="Terpene_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="114"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="Terpene synthase N-terminal" evidence="4">
        <location>
            <begin position="140"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="Terpene synthase metal-binding" evidence="5">
        <location>
            <begin position="265"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9690165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="530" mass="61340" checksum="7541F3FB567A7457" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZLA2</accession>
    <name>A0AA38ZLA2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Zinc beta-ribbon domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_013380</name>
        <name evidence="4" type="ORF">PVL29_013384</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZLA2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056988">
        <property type="entry name" value="Zn_ribbon_pln"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23551">
        <property type="entry name" value="Zn_ribbon_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zinc beta-ribbon" evidence="2">
        <location>
            <begin position="62"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9691182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9691186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="91" mass="10080" checksum="842B6563C1A5A42D" modified="2024-01-24" version="1">MDAKGSQQKAPSQNGVPSASASASGVHNFTSGVASNARTHSNANRPSPTSVPSPSHRRTDTFWTVCNRCKTQYEYHRIYLNHTQAHHVQFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZLI4</accession>
    <name>A0AA38ZLI4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PVL29_013113</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17 23">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 23">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9690792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZLI4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000044">
        <property type="entry name" value="Putative LRR receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.430">
        <property type="entry name" value="Galactose-binding lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051824">
        <property type="entry name" value="LRR_Rcpt-Like_S/T_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021720">
        <property type="entry name" value="Malectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48006">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN DDB_G0281931-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48006:SF66">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11721">
        <property type="entry name" value="Malectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="13 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7 19" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041337235" description="non-specific serine/threonine protein kinase" evidence="19">
        <location>
            <begin position="32"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="431"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="502"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
            <begin position="498"/>
            <end position="811"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAJ9690792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="848" mass="96476" checksum="F96CFAF3E54B2B09" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZPX0</accession>
    <name>A0AA38ZPX0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_012017</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9693105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZPX0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005162">
        <property type="entry name" value="Retrotrans_gag_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33223">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33223:SF11">
        <property type="entry name" value="ELEMENT PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03732">
        <property type="entry name" value="Retrotrans_gag"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Retrotransposon gag" evidence="2">
        <location>
            <begin position="4"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="347"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="362"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="398"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="436"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9693105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="467" mass="53521" checksum="A5ED8301FE6697D4" modified="2024-01-24" version="1">MRLKLFPFTLKDKAKIWLNSLRPRSIRTWTNLQDEFLKKIFPTHRTNGLKRQISNFSAKENEKFYECWERYMEAINACPHHGFDTWLLVSYFYDGMSSSMKQLLETMCGGDFMSKNPEEAMDFLSYVAEVSRGWDEPNRGEVGKMKSQPNASNAKAGMYTLNEDGDMKAKFAAMTRRLEELELKKMHEVQAVAETPMQVKPCSICQSYEHLVEECPTIPVAREMFGDQANVIGQFKPNSNASYGNTYNSSWRNYPNFSWKPRAPQYQQPAQPSQQASNFEQAIVNLSKVVGDFVGDQKSINAQLSQRIENVESTLNKKMDGMQNDLSQKIDNLQYSISRLTNLNTVQEKGRFPSQPHQNPKGIHELETHEGESSQVRDVKALITLRSGKKVESPTPKPHAEEKEEEETKKREEIKGKKKDISKGKEDPDSTMNANLEKELVKEELMKKHISPPFPQALHGKKGIRNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZQH5</accession>
    <name>A0AA38ZQH5_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_012248</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9693396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZQH5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000383">
        <property type="entry name" value="Leucine-rich repeat receptor protein kinase EMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000129">
        <property type="entry name" value="Leucine-rich repeat receptor-like kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000111">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001347">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001646">
        <property type="entry name" value="Putative inactive leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27000">
        <property type="entry name" value="LEUCINE-RICH REPEAT RECEPTOR-LIKE PROTEIN KINASE FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27000:SF809">
        <property type="entry name" value="OS05G0170300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041269726" evidence="13">
        <location>
            <begin position="23"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="960"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="14">
        <location>
            <begin position="37"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="15">
        <location>
            <begin position="108"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="15">
        <location>
            <begin position="313"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9693396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1011" mass="112191" checksum="F73597C40E3A1357" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZS21</accession>
    <name>A0AA38ZS21_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PVL29_009718</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">May play a role in signal transduction pathways that involve calcium as a second messenger.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 22">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 22">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9693890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZS21"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000015">
        <property type="entry name" value="Calcium-dependent protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000004">
        <property type="entry name" value="Calcium-dependent protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001864">
        <property type="entry name" value="Calcium-dependent protein kinase SK5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001294">
        <property type="entry name" value="CDPK-related kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="120"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="19">
        <location>
            <begin position="421"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="19">
        <location>
            <begin position="457"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="19">
        <location>
            <begin position="493"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="19">
        <location>
            <begin position="531"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="37"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00058232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAJ9693890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="584" mass="65767" checksum="16C32C192B9FD5EA" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZS79</accession>
    <name>A0AA38ZS79_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PVL29_009991</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZS79"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05476">
        <property type="entry name" value="pepsin_A_like_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034161">
        <property type="entry name" value="Pepsin-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051708">
        <property type="entry name" value="Plant_Aspart_Prot_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032799">
        <property type="entry name" value="TAXi_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032861">
        <property type="entry name" value="TAXi_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47967:SF14">
        <property type="entry name" value="EUKARYOTIC ASPARTYL PROTEASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47967">
        <property type="entry name" value="OS07G0603500 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14541">
        <property type="entry name" value="TAXi_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14543">
        <property type="entry name" value="TAXi_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041225399" description="Peptidase A1 domain-containing protein" evidence="6">
        <location>
            <begin position="24"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="7">
        <location>
            <begin position="93"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ9694276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="443" mass="48722" checksum="8BEBDCAAF8E0A16F" modified="2024-01-24" version="1">MASVNNLLLIIYLTFIFSPCISAASDSKGFSTNLIHIHSPSSPYKNVKAESLGEDTALKSTLSRHAYIRARQQKALQPEDFVPPPLIPQAIMFLANLSIGNPPTNVYVILDTGSDLFWIQCEPCDVCYKQKDPIYDRTKSDSYTEMACDAPLCLSLGSDAQCSDPAQRFCAYKYSYEDGARTSGLFSYDNVAFTSHYSTEDKTAQVGFGCGLQNLNTVTGYRDGGVLGLGPGQVSLVSQLPAVGKVSKSFAYCFGNVSDPNAGGFLVFGDDTYLNGDMTPMVIDEFYHINLLGIGLGIGEPRLDINSSSFERKPDGSGGVVIDSGSTLSIFVPEVYKVVRNAVVDKLRKGYNIRELRKYPDCFRGKIGRDLEFFPTMVLYLESTGILNDKWSIFRQRYRNAFCLGFTSGDGLNIIGNLAQQSYKFGFNLVQNTLSIESKPNCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZSG1</accession>
    <name>A0AA38ZSG1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_009912</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZSG1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36768">
        <property type="entry name" value="ATP-DEPENDENT HELICASE/DEOXYRIBONUCLEASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36768:SF1">
        <property type="entry name" value="ATP-DEPENDENT HELICASE_DEOXYRIBONUCLEASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041215224" evidence="2">
        <location>
            <begin position="32"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="179"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9694159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="227" mass="25663" checksum="C0BD6F66552510D7" modified="2024-01-24" version="1">MARNCGIAMNGRTMESILVLLFLSLPLFSSAYRPGDIVPMSKMGQYHSSRTVWHDVVGKHCPIFAVNREVLIPIQKPTGYTGADPYKISFQVGREKYLIPWLLLINRKSSEVPMIDVHLRYSGNDLHGVVAKVVDMPHHYVEIHPNIHTQFWNPEHWPKHVLVRYTWEEQSEIDVTSGFYVLFGSGLVMSVVLSIYVLQSSRDKLARFVRETVAEGSVSGAGVAKVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZSJ2</accession>
    <name>A0AA38ZSJ2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_010089</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZSJ2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056715">
        <property type="entry name" value="DUF7813"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36353">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36353:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25105">
        <property type="entry name" value="DUF7813"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="340"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="412"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9694427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="461" mass="52323" checksum="CDFDC14F84057338" modified="2024-01-24" version="1">MTAPPPPPSRLDLRTTTQLLKRTASSFSSTPFTFLLLSLLIFSFRTVVEDGTLILTSFIDRDPSLRALLSRLDIAGKNIPATLSSPPTVNPRHRRRPFLHLTRVGTLDDDFFSGDEYDDRTLFGGGRRTQINGSSLSLSRFDPKLGFSGSVEDNGIRASEIVRSGFSFKGEDLLFTDGNDDDGDEGNQTVVLENKEERGEFPFFIKGMDLGSRDSAALFFLVCFLSAAYGWVILGFLVTYSWVLGIVFISVVNDHLRRTTSFIGTVWDGSRLGLRRLSGFILMRWAVRDGMTQLLGLWFFGEIEDQYSFFKLFVRLKLMPFSVMLPWIRGFEKEISGFLFIWFLWDTLVSFIFAVDAWVAIVDTRRSGRDIVREGVYLLSTMLNQAIQIKCLEAIFCGSFVRWALARFCGKLFAAVFQSVVEVYFMVAWLIFYFAARCRVASTEGRMFGRRELEGFIEGLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZSV4</accession>
    <name>A0AA38ZSV4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box/LRR-repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_009620</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9693749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZSV4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38926:SF5">
        <property type="entry name" value="F-BOX AND LEUCINE-RICH REPEAT PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38926">
        <property type="entry name" value="F-BOX DOMAIN CONTAINING PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9693749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="227" mass="26302" checksum="3EE97BA620F73147" modified="2024-01-24" version="1">MESLLLDVPFVCKLWYKASLDPKCWERLIFPKYIKPDDIWDKSPLGERLMMEYQESFCVTAFIKSPSLKALRLHDDLLFKKSTIIPKLISKWKNLVMLSLGSTHNMEEILALISLHCNNFIMLFAPRIYVGKDEATSIVTSLPNLKYLVLKGSTIERENLVTVLQGCKKLLRLDVRKWIGFEENDAEILALASYIPTFMCKGSILYDYDDEPLTYKDADFAPDYHYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZT89</accession>
    <name>A0AA38ZT89_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Zinc/iron-chelating domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_010190</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZT89"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005358">
        <property type="entry name" value="Puta_zinc/iron-chelating_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36791">
        <property type="entry name" value="OS03G0363400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36791:SF2">
        <property type="entry name" value="OS03G0363400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03692">
        <property type="entry name" value="CxxCxxCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9694594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="188" mass="20677" checksum="808E81DA77A7A94A" modified="2024-01-24" version="1">MALCWCSTMTMMSGPYTQPQKGKGMGMGMGMGMGMGKGKGKGKGKAQTKRRQSRGSDGIGFGGKRNDPTWQCIQGCGACCKLDKGPAFATPEEIFDDPSDVELYRSMVGPDGWCTHYEKSTRTCSIYSDRPYFCRVEPNVFQTLYGIGKKRFNKEACSFCEDTIKAIYGSHSQELDNFVHAVRSTDSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZXY9</accession>
    <name>A0AA38ZXY9_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_009061</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZXY9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.20">
        <property type="entry name" value="Apolipoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58113">
        <property type="entry name" value="Apolipoprotein A-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9697129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="145" mass="15545" checksum="161E71430D98DB58" modified="2024-01-24" version="1">MASLAITITLPKVRQAGSVVVRNRWNSRGFVHCTPRYCQASARDEGSDAADKAVDAVKQGVDATVRGGKEMKDKAASTAEDMTQKTKEVAGKVSETVQDITEKAKQTVQGAWGSVKETVAGKTEEAGECVKDNAETVKRNMNTKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA38ZYM0</accession>
    <name>A0AA38ZYM0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Small ubiquitin-related modifier</fullName>
            <shortName evidence="9">SUMO</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_009225</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="8">Interacts with SAE2, SCE1, SIZ1 and MMS21. Interacts with HSFA2. Covalently attached to ABI5, FLD, GTE3, HSFA2 and ICE1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the ubiquitin family. SUMO subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9697325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA38ZYM0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd16116">
        <property type="entry name" value="Ubl_Smt3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000171">
        <property type="entry name" value="Small ubiquitin-related modifier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022617">
        <property type="entry name" value="Rad60/SUMO-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10562">
        <property type="entry name" value="SMALL UBIQUITIN-RELATED MODIFIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11976">
        <property type="entry name" value="Rad60-SLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="11">
        <location>
            <begin position="26"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00063908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9697325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="112" mass="12279" checksum="7893493D828FA901" modified="2024-01-24" version="1">MSATGGVAGAGTGGQEEDKKPMDQGAHINLKVKGQDGNEVFFRIKRSTQLRKLMSAYCDRQSVELNSIAFLFDGRRLRGEQTPDELEMEDGDEIDAMLHQTGGVAWMCLTAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A0T7</accession>
    <name>A0AA39A0T7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_007787</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A0T7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF78">
        <property type="entry name" value="OS12G0587133 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9698883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="311" mass="35212" checksum="A2F1A20A2B5304A5" modified="2024-01-24" version="1">MRGCLSSVSFVVLVNGNTKGWVKASRGLRQGDPLSPFLFTLVADVLSRMLLRAEERNVLEGFRVGRNRTRVSHLQFADDTIFFSSTQEEDMLTLKSVLLVFGHISGLKVNLDKSNIYGINLGQDHFYRLAELLDCKASGWPILYLGLPLGENPRACGFWDPVIERILSRLDGWQKTYLSFGGRITLIQSCLTHMPCYFLSLFKIPASVTVRIERMQGDFLWSRVGEGKRDHLVSWDVVCIPKAKGGLGLGRISLRNSALLGKWLWRFPREGSALWHQVILSIYGSHSNGWDANTFVRWSHRCPWKVIAQVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A127</accession>
    <name>A0AA39A127_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA helicase</fullName>
            <ecNumber evidence="3">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PVL29_007796</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">ATP-dependent RNA helicase required for 60S ribosomal subunit synthesis. Involved in efficient pre-rRNA processing, predominantly at site A3, which is necessary for the normal formation of 25S and 5.8S rRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="3"/>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A127"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00268">
        <property type="entry name" value="DEADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044742">
        <property type="entry name" value="DEAD/DEAH_RhlB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 14" id="KW-0347">Helicase</keyword>
    <keyword evidence="7 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="18">
        <location>
            <begin position="123"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="16">
        <location>
            <begin position="154"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="17">
        <location>
            <begin position="354"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="13">
        <location>
            <begin position="123"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="35"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ9698897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="568" mass="61765" checksum="3EAAA20CAE2C3F04" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A2C2</accession>
    <name>A0AA39A2C2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phospholipase</fullName>
            <ecNumber evidence="6">3.1.4.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_005502</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1,2-diacyl-sn-glycero-3-phosphate + choline + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14445"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="3.1.4.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the phospholipase D family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.4" evidence="6"/>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9699663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A2C2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004630">
        <property type="term" value="F:phospholipase D activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009395">
        <property type="term" value="P:phospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01254">
        <property type="entry name" value="PH_PLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09138">
        <property type="entry name" value="PLDc_vPLD1_2_yPLD_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09141">
        <property type="entry name" value="PLDc_vPLD1_2_yPLD_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.870.10:FF:000011">
        <property type="entry name" value="Phospholipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025202">
        <property type="entry name" value="PLD-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016555">
        <property type="entry name" value="PLipase_D_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015679">
        <property type="entry name" value="PLipase_D_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18896:SF76">
        <property type="entry name" value="PHOSPHOLIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18896">
        <property type="entry name" value="PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00614">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13091">
        <property type="entry name" value="PLDc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009376">
        <property type="entry name" value="Phospholipase_D_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00155">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PH" evidence="8">
        <location>
            <begin position="250"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="9">
        <location>
            <begin position="476"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="9">
        <location>
            <begin position="911"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="646"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="652"/>
            <end position="668"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR009376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9699663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1115" mass="127100" checksum="630470C51890FB16" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A535</accession>
    <name>A0AA39A535_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_006467</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000005">
        <property type="protein sequence ID" value="KAJ9701130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A535"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9701130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="54" mass="6109" checksum="2C81D7175A65AB83" modified="2024-01-24" version="1">MKDEANESVSAGLSYSIEIKDAYIPPWIISSICATMGSEWRSFEARYDKFCHCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A7S0</accession>
    <name>A0AA39A7S0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RCC1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_004164</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A7S0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058923">
        <property type="entry name" value="RCC1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051210">
        <property type="entry name" value="Ub_ligase/GEF_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22870:SF155">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HERC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22870">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25390">
        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="RCC1-like" evidence="3">
        <location>
            <begin position="35"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="61"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="123"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="186"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="239"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9702289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="453" mass="49601" checksum="FCD2726CB01D315E" modified="2024-01-24" version="1">MDGQLGFSDGNSLVPHLLELFLELGSPGSLTDESDTKNKTPLKVNSVKAGGMMSLAIDNLGGLWMWGNVPENSPNKGEFSLVSSFTPIPVWDFHGHTVVKVACGNEHVVALVSHGETYKGGDLVCYSWGNNVHGQLGLGDTENRLRPEVVKTFNHESLWEVFEVACGAFHTALLTHRKRPSDTLESMCWTFGQGDNGQLGHGTAKGLSLPEPVKDLPEHIFLVSVDCGLFHTSVVSLAGEVWSWGMEKGLGLCPDASFTGTDPGDAPSPLLYGSKFRDPVQVACGAAHTVLVADDGYKLWSWGRGRSGVLGNGKTVDCFAPSTVLWPPLTEDFKELESGTTSKEKKIENKDPEEEMKLLHSKLSTMERYASILHGSIFGKPFEEQDIPISLQNSGFFDIAKEWENMLESADRGKLVRLEKFYRNMLAGVKDKLMKRRIQEIIKECLPPPATGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A8G5</accession>
    <name>A0AA39A8G5_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_004203</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A8G5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000324">
        <property type="entry name" value="Transcription factor MYB3R-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000010">
        <property type="entry name" value="Transcriptional activator Myb isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000016">
        <property type="entry name" value="Transcriptional activator Myb isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF266">
        <property type="entry name" value="TRANSCRIPTION FACTOR MYB3R-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="32"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="32"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="84"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="84"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="136"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="140"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="212"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="526"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="663"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="738"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="810"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="870"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1019"/>
            <end position="1053"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="29"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="214"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="751"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="816"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="873"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1033"/>
            <end position="1046"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9702357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="1053" mass="115749" checksum="37C365AFB615F695" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A8Y8</accession>
    <name>A0AA39A8Y8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NB-ARC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_004752</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A8Y8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766:SF40">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766">
        <property type="entry name" value="PLANT BROAD-SPECTRUM MILDEW RESISTANCE PROTEIN RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0611">Plant defense</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NB-ARC" evidence="2">
        <location>
            <begin position="2"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9703112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="144" mass="16265" checksum="C14EA77E78D1F738" modified="2024-01-24" version="1">MIARVGLGGLGETTLAQLLYNDEMIKSYFDMKAWVCVSEEFDPLRITKSILEAINAPYLSCISDIKDLNLLQVELKECLNGKKFLLILDDVWNESYNNWDMLQTPLKFGTSGSKFIVTMCTANVALTMHAHHTHLLGQLCFEDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A913</accession>
    <name>A0AA39A913_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_004607</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A913"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48434">
        <property type="entry name" value="(RAPE) HYPOTHETICAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48434:SF1">
        <property type="entry name" value="(RAPE) HYPOTHETICAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="191"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9702934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="243" mass="29110" checksum="36AA3698421E3F74" modified="2024-01-24" version="1">MNITDWNQKPFTSKTFSQPYHPMSYNYIDYMDAWYNMFYLQSYQHSWFIQFSRNCNTQFLAWFKKWWTFFGLLDSIFPPDIQEKFEFYKKNTSSQPFSQPKLLMFCSRLRIQWILTGNIIKKQEQPQPFPLSLSREFNIKWWDKFNHDFVCQQKVLEIISARLGSSNSSQGPTQEEFLTLKSKCQALLAAATDLEQYRQELLQTLKQFEDQKENEENEEIVISDDSYVDPEFIIYGGPMGQAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A9L8</accession>
    <name>A0AA39A9L8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="1">2.3.2.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_004196</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="1"/>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9702344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A9L8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23812">
        <property type="entry name" value="UBCc_ScPEX4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000044">
        <property type="entry name" value="protein PEROXIN-4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0962">Peroxisome biogenesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 8" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="9">
        <location>
            <begin position="8"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="7">
        <location>
            <position position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9702344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="162" mass="18256" checksum="B1EAE018F6B007C9" modified="2024-01-24" version="1">MQPPIPQASRARLFKEYKEVQREKAADPDIQLVCDDSNIFKWTALIKGPSETPFEGGVFQLAFAVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDIRGYQSMARMYTRLAAMPKKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39A9X2</accession>
    <name>A0AA39A9X2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Response regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_004785</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39A9X2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052048">
        <property type="entry name" value="ST_Response_Regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43228">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43228:SF1">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR ARR22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="12"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9703152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="130" mass="14254" checksum="47700FCC228EFEB1" modified="2024-01-24" version="1">MRGKGSSSSKISTLIVDDDPIIRRIHKVLLTKVGLETQVVENGKKAVDLYHSGASFDLILMDLEMAIMDGPKATKELRAMGVQSMIVGVTARDLEVEKQMFMASGLNDCYVKPLTTDTVTALVKKLKKDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ABE5</accession>
    <name>A0AA39ABE5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_004887</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000004">
        <property type="protein sequence ID" value="KAJ9703274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ABE5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039537">
        <property type="entry name" value="Retrotran_Ty1/copia-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057670">
        <property type="entry name" value="SH3_retrovirus"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648:SF31">
        <property type="entry name" value="RNA-DIRECTED DNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648">
        <property type="entry name" value="TRANSPOSASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25597">
        <property type="entry name" value="SH3_retrovirus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="2">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="204"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="212"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="260"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9703274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="270" mass="30775" checksum="81517B7E9C78447A" modified="2024-01-24" version="1">MVQNQFGVKIKSFRTDNARDYFNQTLSPYFQSQGILHDSSCVNTPQQNGVIERKNEHLLNTTRVLLFQGNVPKSYWGEAILTATYMINKIPSRVSENKSPIEILKSFYPHFRTSNGFTPRVFGCTAFVHVHSQHRDKLDPRAIKFVFLGYSSTKKGYKCYNSSTRKFYISADVTFTENERFFPKSSLQGEISMMEDSPYKSFEPLDLSHVSTHGDEEPKSSEPESSESITPESPNFTTEPVSSPVPASVTRNFPQVPKVYSREKAIPEQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39ADE1</accession>
    <name>A0AA39ADE1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_003474</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39ADE1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36381">
        <property type="entry name" value="ETHYLENE-REGULATED TRANSCRIPT 2 (ERT2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36381:SF1">
        <property type="entry name" value="ETHYLENE-REGULATED TRANSCRIPT 2 (ERT2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9705452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="60" mass="6515" checksum="C478C90CC52C1987" modified="2024-01-24" version="1">MSVVVILALGTKKLAVGITMSAFLLLFLEYAAKWVFGLLTLCSDAQIAIKSLIPKVFPFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AE59</accession>
    <name>A0AA39AE59_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_003559</name>
        <name evidence="11" type="ORF">PVL29_003565</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JARBHA010000003">
        <property type="protein sequence ID" value="KAJ9705565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AE59"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013695">
        <property type="entry name" value="WAK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025287">
        <property type="entry name" value="WAK_GUB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33491">
        <property type="entry name" value="OSJNBA0016N04.9 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13947">
        <property type="entry name" value="GUB_WAK_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08488">
        <property type="entry name" value="WAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041630218" evidence="7">
        <location>
            <begin position="22"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="Wall-associated receptor kinase galacturonan-binding" evidence="9">
        <location>
            <begin position="29"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="Wall-associated receptor kinase" evidence="8">
        <location>
            <begin position="158"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9705559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9705565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="268" mass="29292" checksum="4F50437A1640EC2F" modified="2024-01-24" version="1">MSTVQWLLQISLLLWLSKGLAATHGKPGCQERCGDVDNPYPFGIGGVGCYFDEWFEVTCNNSTNPPKPFLKGINLEVLNVSLDGTIRVNNPVLLSQNCSGKPSNDTQWWEGGPFSFSDTYTRFTAVGCSALAYFMQDDTVIGGCMTFCEKDATAAKKASCYGLECCQTQVPPGLLSFTADLDTFSDGSPDEQEPCKYAFMVDQEWFISNVPDPHTVKDMEYVPAFLCNNTLCSTDTQTSSLTCSCLPGHEGNPYLPQGCQVTQSTEDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AEG2</accession>
    <name>A0AA39AEG2_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_001606</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AEG2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009424">
        <property type="entry name" value="AGP16/20/22/41"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33374">
        <property type="entry name" value="ARABINOGALACTAN PROTEIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06376">
        <property type="entry name" value="AGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041348615" evidence="2">
        <location>
            <begin position="26"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9706125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="61" mass="6331" checksum="0EBD7DEE1DD6492B" modified="2024-01-24" version="1">MAVSKVVLMALAVLVFVVALPAVRAQSIAPAPAPTSDGITIDQGIAYVLMLLALVLTYLIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AGP7</accession>
    <name>A0AA39AGP7_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_002347</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AGP7"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052160">
        <property type="entry name" value="Gypsy_RT_Integrase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47266">
        <property type="entry name" value="ENDONUCLEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="39"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9707316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="349" mass="40553" checksum="0E2415B456DFCF7D" modified="2024-01-24" version="1">MKVIQSGFWWPSLFKDAHSMCKGCDRCQRLGKLTRRNMMPLNPILIVDVFDVWGIDFMGPFPMSFGHSYILVGVDYVSKWVEAIPCRSNDHKVVLKFLKENIFSRFGVPKAIISDGGTHFCNKPFETLLAKYGVKHKVATPYHPQTSGQVELANREVKNILMKVVNVNRKDWSIKLLDSLWAYRTAYKTILGMSPYRLVYGKACHLPVEIEYKAWWAIKKLNMDLTRAGLKRCLDLNELEEMRNDAYLNSKISKERLKKWHDQLVNQKNFAKGQRVLLYDSKLHLFPGKLKSRWTGPFIIHDVQPNGVVELLNFNSTRTFKVNGHRLKPYMESFSRDKEEFILLDPPPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AH15</accession>
    <name>A0AA39AH15_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Retrotransposon Copia-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PVL29_002495</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9707490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AH15"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ9707490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="90" mass="10058" checksum="66680D70671CABC0" modified="2024-01-24" version="1">MVDWFSSSSTNPVVLTIGNNLPSQPNTLISINVVTQMPFKLNKMNYASWRAQFTNLLFGYDLLGFLDDTTPYPPKTILQSSSTMPISIPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AHW2</accession>
    <name>A0AA39AHW2_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">allantoate deiminase</fullName>
            <ecNumber evidence="12">3.5.3.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_000114</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>allantoate + H2O + 2 H(+) = (S)-2-ureidoglycine + NH4(+) + CO2</text>
            <dbReference type="Rhea" id="RHEA:27485"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17536"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:59947"/>
            <dbReference type="EC" id="3.5.3.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M20A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.3.9" evidence="12"/>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9707882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AHW2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047652">
        <property type="term" value="F:allantoate deiminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006144">
        <property type="term" value="P:purine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03884">
        <property type="entry name" value="M20_bAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.360:FF:000019">
        <property type="entry name" value="Allantoate deiminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010158">
        <property type="entry name" value="Amidase_Cbmase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001261">
        <property type="entry name" value="ArgE/DapE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01879">
        <property type="entry name" value="hydantase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32494">
        <property type="entry name" value="ALLANTOATE DEIMINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32494:SF19">
        <property type="entry name" value="ALLANTOATE DEIMINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00758">
        <property type="entry name" value="ARGE_DAPE_CPG2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0464">Manganese</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0659">Purine metabolism</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="13">
        <location>
            <begin position="270"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00053003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00066382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9707882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="492" mass="53022" checksum="B733DCD931C8A04D" modified="2024-01-24" version="1">MAHSSVRRISMPQMMIVVVTSSSSIFSLCFALSLLSFFASAHILTDTEGKNSDLFPEVLREEAVARLYELGKVSDADGYLERTFLSPASIRAGNLIREWMEDAGLRTWMDQMGNVHGRVEGMNPSADALLIGSHLDTVVDAGIFDGSLGIISAISALKVLNITAKLRKLRRPIEVIAFSDEEGVRFQSTFLGSAAVAGVLPASALQISDKSGVTVQNALMDNSIEATEETLSQLKYDPKTVWGYVEVHIEQGPVLEGIGLPLAVVKGIAGQTRLKVTVRGSQGHAGTVPMSMRQDPMAAAAELIVLLESLCKRPQDFLSYDGQCNGFAVESLSGSLVCTVGEISSWPSASNVIPGQVTFTVDLRAIDDMGREAVLYELSDRMYQICEKRSISCTIERKHDANAVICDNELSTQLKSATYSALKRMAGEIQGDVPVLMSGAGHDAMAMSHLTKVGMLFVRCRGGISHSPAEHVLDEDVWAAGLAILAFLETHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AIA1</accession>
    <name>A0AA39AIA1_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_002045</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000002">
        <property type="protein sequence ID" value="KAJ9706888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AIA1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35307:SF3">
        <property type="entry name" value="DUF4220 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35307">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="155"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="226"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="272"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="371"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9706888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="749" mass="84970" checksum="26F9B5326B9BC9EF" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AIC3</accession>
    <name>A0AA39AIC3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_000300</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the AP2/ERF transcription factor family. ERF subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9708171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AIC3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000005">
        <property type="entry name" value="ethylene-responsive transcription factor RAP2-11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050913">
        <property type="entry name" value="AP2/ERF_ERF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31194:SF62">
        <property type="entry name" value="ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR ERF118"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31194">
        <property type="entry name" value="SHN SHINE , DNA BINDING / TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00367">
        <property type="entry name" value="ETHRSPELEMNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="8">
        <location>
            <begin position="102"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="59"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="158"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="158"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9708171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="316" mass="35195" checksum="8C5257A5885AAC8C" modified="2024-01-24" version="1">MPEPRRQPMIQDMPKEICSDFKMRKIRVICYDPEATDSSSSEDEGGICKREKRGKRIVREINLPVAGSPQSNGIETESSCQDSNNGGKNPKKRVLRKPPSSKYRGVRQRKWGKWAAEIRDPFRGVRVWLGTYNTPEEAAKAYESKRLEFEAMAASANKSQNVSSSVVVSQSQSHNPAVSDDSDSLLSHTSPSSVLELETSTSHYNGGGHGTDATKEGVGTNLIHANGAEQQKPISDFAEEPLMAPPIGQELNMEAELESFFDDNNWGRILDDFNCLDDLRIFGFDNEEPSDLPDFDFDLGNDEFAWMDEPLNISCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39AKI6</accession>
    <name>A0AA39AKI6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Uncoupling protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PVL29_001021</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000001">
        <property type="protein sequence ID" value="KAJ9709350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39AKI6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000062">
        <property type="entry name" value="mitochondrial uncoupling protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050391">
        <property type="entry name" value="Mito_Metabolite_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45618">
        <property type="entry name" value="MITOCHONDRIAL DICARBOXYLATE CARRIER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="10"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="102"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="203"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ9709350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="299" mass="33121" checksum="DCFDFA60DFD133B5" modified="2024-01-24" version="1">MKPRNDETYTKIALTSLSAMVAETSTFPIDLTKTRLQLHGESLSSTRSTTAFRVAAEIVRRDGPLGLYKGLSPAILRHLFYTPIRIVGYEHLRNAVDGHDSVSLTGKALVGGISGVIAQVVASPADLVKVRMQADGRMVSQGLQSRYSGTFDALNKIIRTEGFRGLWKGVFPNVQRAFLVNMGELACYDHAKYFVIQNQICGDNIYSHTLASIMSGLAATALSCPADVVKTRMMNQAVSKEGKSMYNNSYDCLVKTVRVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRELAGLSSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D7G0</accession>
    <name>A0AA39D7G0_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_023939</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D7G0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="40"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9674708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="94" mass="10387" checksum="DA29A11493902D3D" modified="2024-01-24" version="1">MTTNLRTNFKERQRKCLFEPIAVAREEPATDIPPMPMPPADATGSSSAQAATSLTKNKRLEVIEAMRAFITQRIGGSEELRAKLEWVESNLAAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39D7U5</accession>
    <name>A0AA39D7U5_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ACB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PVL29_023672</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ACBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000018">
        <property type="protein sequence ID" value="KAJ9674261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39D7U5"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000062">
        <property type="term" value="F:fatty-acyl-CoA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000582">
        <property type="entry name" value="Acyl-CoA-binding_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035984">
        <property type="entry name" value="Acyl-CoA-binding_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23310:SF122">
        <property type="entry name" value="ACYL-COA-BINDING DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23310">
        <property type="entry name" value="ACYL-COA-BINDING PROTEIN, ACBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00887">
        <property type="entry name" value="ACBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00689">
        <property type="entry name" value="ACOABINDINGP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47027">
        <property type="entry name" value="Acyl-CoA binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51228">
        <property type="entry name" value="ACB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041467824" description="ACB domain-containing protein" evidence="4">
        <location>
            <begin position="31"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="ACB" evidence="5">
        <location>
            <begin position="139"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="226"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="231"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="248"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="279"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ9674261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="294" mass="32166" checksum="CEF7DB0334697979" modified="2024-01-24" version="1">MELVQELLLTVSLALLFSFLVAKLVSLAMAADMKSAQNVDDHIVANEVRFVEIETRVESVDGGSGLVEETCEITRGGEVPVLPSEEEVSEIVELPENSPRVRSGHEILVSHDVAVVEEREERELESEGDDWEGVERSELERVFAAAANFVGSGNKGDGWSSDAQMELYGLHKIAVEGPCHEPQPMPLKLSARAKWNAWQRMGNMSREVAMEQYIALLSDRVPGWMEASSAGDDKPESSEGGVHDILDPNQSSLLHDQISSTNETKPELKSGTVGIDLTEGSNSVRTCDEQNSSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DDT9</accession>
    <name>A0AA39DDT9_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyanobacterial aminoacyl-tRNA synthetase CAAD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PVL29_020256</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9681293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DDT9"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025564">
        <property type="entry name" value="CAAD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033344">
        <property type="entry name" value="CURT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33222">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33222:SF4">
        <property type="entry name" value="PROTEIN CURVATURE THYLAKOID 1A, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14159">
        <property type="entry name" value="CAAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="99"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="129"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="Cyanobacterial aminoacyl-tRNA synthetase CAAD" evidence="3">
        <location>
            <begin position="86"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ9681293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="170" mass="18163" checksum="4EAEE541C5ADF7EB" modified="2024-01-24" version="1">MAMPYVAVASSSMAATPVLMPRLPATATATAAANAAARCSALPYLPPRLSASSNHFSGSRRFSSLQVRASSSEETSLNTEELFTDLKGKWDALENKSTVLLYGGGAIVAVWLSSVVVGAINSVPLVPKIMELVGLGYTAWFVYRYLLFKSSRKELATDIEELKKKVTGAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DDU1</accession>
    <name>A0AA39DDU1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein uL3c</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_019381</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the universal ribosomal protein uL3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000015">
        <property type="protein sequence ID" value="KAJ9680079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DDU1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009941">
        <property type="term" value="C:chloroplast envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.810:FF:000001">
        <property type="entry name" value="50S ribosomal protein L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000065">
        <property type="entry name" value="50S ribosomal protein L3, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.810">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01325_B">
        <property type="entry name" value="Ribosomal_uL3_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000597">
        <property type="entry name" value="Ribosomal_uL3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019927">
        <property type="entry name" value="Ribosomal_uL3_bac/org-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019926">
        <property type="entry name" value="Ribosomal_uL3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03625">
        <property type="entry name" value="L3_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11229">
        <property type="entry name" value="50S RIBOSOMAL PROTEIN L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11229:SF16">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00297">
        <property type="entry name" value="Ribosomal_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00474">
        <property type="entry name" value="RIBOSOMAL_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="204"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9680079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="281" mass="30762" checksum="1975584E59AF9BB2" modified="2024-01-24" version="1">MAVTASLSCIRLPLSPTTQHFPSHSLSLSSSFTLNPLQTHLQLRLQPFRSAKSTLPIIRMSMEAGVGVMATKLGMMSYFEPEGKVVPVTVVGFREGNIVTQVKTEATDGYSAVQVGYRRVRDRKLTKPEMGHLEKAGTIPMRHLQEFRLVSVDGFEPNQKLVFEELFKEGDLVDVSGITIGKGFQGGIKRHNFRRGLMTHGSKSHRALGSIGAGTTPGHVYKGKKMPGRMGGTKTKIRKLKIVKIDNDLRVVMIKGAVPGKPGNLLRITPAKIVGKNIPKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DE88</accession>
    <name>A0AA39DE88_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pectinesterase</fullName>
            <ecNumber evidence="7">3.1.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_021047</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Acts in the modification of cell walls via demethylesterification of cell wall pectin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>[(1-&gt;4)-alpha-D-galacturonosyl methyl ester](n) + n H2O = [(1-&gt;4)-alpha-D-galacturonosyl](n) + n methanol + n H(+)</text>
            <dbReference type="Rhea" id="RHEA:22380"/>
            <dbReference type="Rhea" id="RHEA-COMP:14570"/>
            <dbReference type="Rhea" id="RHEA-COMP:14573"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17790"/>
            <dbReference type="ChEBI" id="CHEBI:140522"/>
            <dbReference type="ChEBI" id="CHEBI:140523"/>
            <dbReference type="EC" id="3.1.1.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Secreted</location>
            <location evidence="7">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the pectinesterase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the PMEI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.11" evidence="7"/>
    <dbReference type="EMBL" id="JARBHA010000016">
        <property type="protein sequence ID" value="KAJ9679012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DE88"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004857">
        <property type="term" value="F:enzyme inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030599">
        <property type="term" value="F:pectinesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042545">
        <property type="term" value="P:cell wall modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045490">
        <property type="term" value="P:pectin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd15798">
        <property type="entry name" value="PMEI-like_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.40:FF:000013">
        <property type="entry name" value="Pectinesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000001">
        <property type="entry name" value="Pectinesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.40">
        <property type="entry name" value="Invertase/pectin methylesterase inhibitor family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035513">
        <property type="entry name" value="Invertase/methylesterase_inhib"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033131">
        <property type="entry name" value="Pectinesterase_Asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000070">
        <property type="entry name" value="Pectinesterase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006501">
        <property type="entry name" value="Pectinesterase_inhib_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018040">
        <property type="entry name" value="Pectinesterase_Tyr_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01614">
        <property type="entry name" value="PME_inhib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31707">
        <property type="entry name" value="PECTINESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01095">
        <property type="entry name" value="Pectinesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04043">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00856">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101148">
        <property type="entry name" value="Plant invertase/pectin methylesterase inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00800">
        <property type="entry name" value="PECTINESTERASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00503">
        <property type="entry name" value="PECTINESTERASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0063">Aspartyl esterase</keyword>
    <keyword evidence="7" id="KW-0134">Cell wall</keyword>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041488688" description="Pectinesterase" evidence="7">
        <location>
            <begin position="24"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="domain" description="Pectinesterase inhibitor" evidence="8">
        <location>
            <begin position="25"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ9679012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="520" mass="57814" checksum="719DEEE2AD10FD09" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DFD6</accession>
    <name>A0AA39DFD6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_018739</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="JARBHA010000014">
        <property type="protein sequence ID" value="KAJ9682868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DFD6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001023">
        <property type="entry name" value="Os07g0541700 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052059">
        <property type="entry name" value="CR_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47973">
        <property type="entry name" value="CYSTEINE-RICH RECEPTOR-LIKE PROTEIN KINASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9682868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="85" mass="9953" checksum="13E764717923DDD4" modified="2024-01-24" version="1">MYFNVDKSKSSLLDWPKRYNIILGVARGLFYLHEDSQYRIIHRDIKASIILLDKEMNPKISDFGLAKLFEDEQSHHQTRCIAGTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DHP0</accession>
    <name>A0AA39DHP0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 18">Peroxidase</fullName>
            <ecNumber evidence="4 18">1.11.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PVL29_016464</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:56136"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:139520"/>
            <dbReference type="ChEBI" id="CHEBI:139521"/>
            <dbReference type="EC" id="1.11.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 18">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="15 18">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 18">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="15 18">Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="18">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peroxidase family. Ascorbate peroxidase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.7" evidence="4 18"/>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9683980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DHP0"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140825">
        <property type="term" value="F:lactoperoxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00693">
        <property type="entry name" value="secretory_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.420.10:FF:000006">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.420.10">
        <property type="entry name" value="Peroxidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000823">
        <property type="entry name" value="Peroxidase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019794">
        <property type="entry name" value="Peroxidases_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019793">
        <property type="entry name" value="Peroxidases_heam-ligand_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033905">
        <property type="entry name" value="Secretory_peroxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31388:SF24">
        <property type="entry name" value="PEROXIDASE 52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31388">
        <property type="entry name" value="PEROXIDASE 72-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00458">
        <property type="entry name" value="PEROXIDASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00461">
        <property type="entry name" value="PLPEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00435">
        <property type="entry name" value="PEROXIDASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00436">
        <property type="entry name" value="PEROXIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50873">
        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15 18" id="KW-0106">Calcium</keyword>
    <keyword evidence="11 17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 18" id="KW-0349">Heme</keyword>
    <keyword evidence="18" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="10 15 18" id="KW-0408">Iron</keyword>
    <keyword evidence="7 15 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 18" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5 18" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="18" id="KW-0964">Secreted</keyword>
    <keyword evidence="18" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041481532" description="Peroxidase" evidence="18">
        <location>
            <begin position="26"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="Plant heme peroxidase family profile" evidence="19">
        <location>
            <begin position="26"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="67"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="15">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="16">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="36"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="69"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="122"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="232"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU362060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAJ9683980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="352" mass="37905" checksum="DE0E79B92AF6F2F7" modified="2024-01-24" version="1">MASPSSYMAIVTTALLILFLGSSTAQLSINYYSKSCPKLFPTVKSAVKSAIAKEARMGASLLRLFFHDCFVNGCDGSVLLDDTSSFIGEKNAAPNANSARGFNVIDDIKSKVEKACPGVVSCADVLAITARDSVVIVSLALLIISIIFQNQAYFPLNMCFSCNGLLQLGGPSWNVKLGRRDARTASQAAANNSIPPPTSNLNQLISRFQALGLSTRDLVALAGSHTIGQARCTSFRARIYNETNIDKSFAKTRQSNCPRASGSGDNNLAPLDLQTPTAFENNYYKNLIKKKGLLHSDQQLFNGGSTDSIVRKYSNSRSNFNADFVAGMIKMGDISPLTGSNGEIRKNCRRVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2025-10-08" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DHW0</accession>
    <name>A0AA39DHW0_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">ABC transporter B family member 20</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_016590</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>(indol-3-yl)acetate(in) + ATP + H2O = (indol-3-yl)acetate(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:84235"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:30854"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:84236"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9684175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18577">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18578">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000028">
        <property type="entry name" value="ABC transporter B family member 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000240">
        <property type="entry name" value="ABC transporter B family member 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001683">
        <property type="entry name" value="ABC transporter B family member 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000021">
        <property type="entry name" value="ABC transporter B family member 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000049">
        <property type="entry name" value="ABC transporter B family member 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24222">
        <property type="entry name" value="ABC TRANSPORTER B FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24222:SF52">
        <property type="entry name" value="ABC TRANSPORTER B FAMILY MEMBER 20-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="77"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="125"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="202"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="227"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="301"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="817"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="880"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="966"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1056"/>
            <end position="1083"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1103"/>
            <end position="1124"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="15">
        <location>
            <begin position="81"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="14">
        <location>
            <begin position="402"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="15">
        <location>
            <begin position="829"/>
            <end position="1129"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="14">
        <location>
            <begin position="1160"/>
            <end position="1397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="665"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="694"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="757"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="791"/>
            <end position="804"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00093214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9684175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1410" mass="156218" checksum="2F800EF84D8EF709" modified="2024-01-24" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DIS3</accession>
    <name>A0AA39DIS3_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_017668</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000013">
        <property type="protein sequence ID" value="KAJ9685721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DIS3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36373">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36373:SF1">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="92"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="281"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="169"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="110"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9685721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="353" mass="40392" checksum="53339B5123D13B11" modified="2024-01-24" version="1">MEPAKIDWKTLDSRFVEDELYEHIHAPQWVDFNAPQEFVDDEAWFCRPDCKHPKTVEDFLISTPNSKVLSSADKSETLPLGDWNRRDAKLKRRGFAHQSSMPEFTEDGENQNPNFSTPPHHQPKSMKTKSSSEKKKQIKPGAQKDETPRLKSTLSARNLFAGRDILNQISEFCHELKKLATRAREEENEEKVNLKKSPVGTKKPVVKEVTSEGLNDKERERKPLLEVGKQKSQPIEKNKIKQKLRKKREDEAENIPISLDLKNVKPKEESLLQIRTCPPTPQCFSAARGPNKSTPPKAARSRRMERGLLQEIEQNNKEGDKGNNGTTVSIIAEREAKTTVDMFWFLKPCTLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DJL1</accession>
    <name>A0AA39DJL1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PVL29_015334</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the AP2/ERF transcription factor family. ERF subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9686379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DJL1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009873">
        <property type="term" value="P:ethylene-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000001">
        <property type="entry name" value="Ethylene-responsive transcription factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051758">
        <property type="entry name" value="ERF/AP2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31657:SF19">
        <property type="entry name" value="ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR ERF053"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31657">
        <property type="entry name" value="ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR ERF061"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00367">
        <property type="entry name" value="ETHRSPELEMNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0936">Ethylene signaling pathway</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="10">
        <location>
            <begin position="220"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="283"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="423"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="13"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="66"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="307"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="362"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ9686379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="465" mass="51431" checksum="FBE4468E08F54269" modified="2024-01-24" version="1">MDAAKHGGKSKKSVGETRDMVGRERSKAGDFDLSLERRQWKPVFGEASMSNRPLKKIRSPERRDSSLSSSSSSLAYQPPSPSLPSTVSASSPTSLNLPPPSSRIVFPFAFDGSQQMEIPQQFRTAPLAMFPPPPMQQTSQNQQQMISFAPQHHHSMAGYPAFLAGDLGLQQQQQQQQLLQYWSDALNLSPRGRMMMMNRLSQDGRNFFRPQAPPIATTKLYRGVRQRHWGKWVAEIRLPRNRTRLWLGTFDTAEDAALAYDREAFKLRGENARLNFPELFLNKDKAGSNAPSSSSSSPPTPHENSLRGRSQNQPQQAPEGLNLQDSEVGMLPPPAPSPGYDSNNELGLGLGSSEAAARNEVQKSSTAGESVSESPPELVWGEMAEAWFNAIPAGWGPGSAVWDDLDTSNNLLLQSNLHFGNAHQQQQEAFNDPDSGRQQDNLDSASSSSSSCPMRPFFWRDQDHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DKG8</accession>
    <name>A0AA39DKG8_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_015941</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000012">
        <property type="protein sequence ID" value="KAJ9687264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DKG8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9687264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="113" mass="12209" checksum="988DA4A0B30FFC67" modified="2024-01-24" version="1">MLYNSVGFNWLACPAATELEMARGVGGSSRVVGISPARTTIVGGKKEKLNRTVRREPFGSSDSPVGPPVYPVQFRSDHSPVQLTRPNRNPDRPTVRPVGPAGPVRFLKPCLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DLI1</accession>
    <name>A0AA39DLI1_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Beta-amyrin 28-monooxygenase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Beta-amyrin 28-oxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PVL29_014721</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>beta-amyrin + 3 reduced [NADPH--hemoprotein reductase] + 3 O2 = oleanolate + 3 oxidized [NADPH--hemoprotein reductase] + 4 H2O + 4 H(+)</text>
            <dbReference type="Rhea" id="RHEA:43068"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:10352"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:82828"/>
            <dbReference type="EC" id="1.14.14.126"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9689198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DLI1"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102373">
        <property type="term" value="F:beta-amyrin 28-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016709">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016125">
        <property type="term" value="P:sterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11043">
        <property type="entry name" value="CYP90-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000022">
        <property type="entry name" value="Taxadiene 5-alpha hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286">
        <property type="entry name" value="CYTOCHROME P450 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286:SF384">
        <property type="entry name" value="P450, PUTATIVE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 14 15" id="KW-0349">Heme</keyword>
    <keyword evidence="9 14 15" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 14 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 15" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="8 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="binding site" description="axial binding residue" evidence="14">
        <location>
            <position position="416"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00052804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00073578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00083450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ9689198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="467" mass="52991" checksum="920661A549E4043E" modified="2024-01-24" version="1">MALSLSFWTRFFVHQLEKSSGINLPPGKMGFPFIGESLEFLRMGRKGTPERFIQDRMAKYSTQIFKTCLLGEPTAVVCGAAGNKLLFSNENKLVTSWSPRSVERIFLSSRQTSTKEESMKTRKLLPGFLKPEALQKYVGIMDSIAKWHLDNHWDLNETVTVFPLAKQYTFMVACRLFLSIDDPKHIAKFANPFHILAAGVMSIPINFPGTPFNRAIKAANTVRKELRAIIKQRKINVLAGKSSSSKHDILSHMLTTTDENGQFLNEMDIADKILGLLIGGHDTASAVITFIIKYLAELPQVYNEVLKEQIEVAAGKQSGEMLDWEDIQKMKYSWNVANEVMRLAPPLQGSFREAVTDFTYAGFSIPKGWKLYWSTNATHKNPDYFPDPEKFDPSRFEGNGPIPYTYVPFGGGPRMCPGKEYARLEILVFIHNVVRRFSWYKLHPNEDVIVDPMPMPAKGLPIRLRHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DMD3</accession>
    <name>A0AA39DMD3_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mitochondrial pyruvate carrier</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_012507</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Mediates the uptake of pyruvate into mitochondria.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion inner membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the mitochondrial pyruvate carrier (MPC) (TC 2.A.105) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9689876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DMD3"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006850">
        <property type="term" value="P:pyruvate import into mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005336">
        <property type="entry name" value="MPC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14154">
        <property type="entry name" value="UPF0041 BRAIN PROTEIN 44-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03650">
        <property type="entry name" value="MPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="39"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9689876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="193" mass="22250" checksum="353A5CD2B0316791" modified="2024-01-24" version="1">MSCEKSSFILFYIFKTAHGPKPNRIMKIVCFTDSKSSTSLRICLVDSIFVFIFFYIVLIILRRTIYIDCRTNWWYYSERALGLLELLDTSLRQPIIVLISTPQIHFWAPTFKWGISIANVADFAKPPEKLSYPQQIAVTCTGVIWSRYSTVITPKNWNLFSVNVAMAGTGIYQLSRKIKHDYFPEPEAAVAEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DNI4</accession>
    <name>A0AA39DNI4_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myb/SANT-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_013443</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000010">
        <property type="protein sequence ID" value="KAJ9691268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DNI4"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024752">
        <property type="entry name" value="Myb/SANT-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46250">
        <property type="entry name" value="MYB/SANT-LIKE DNA-BINDING DOMAIN PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46250:SF15">
        <property type="entry name" value="OS01G0523800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12776">
        <property type="entry name" value="Myb_DNA-bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb/SANT-like" evidence="2">
        <location>
            <begin position="19"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="182"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="182"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9691268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="291" mass="32807" checksum="7CA5873770B82B69" modified="2024-01-24" version="1">MDSTSSGQIRGRGRNKRVWTPGEDEKLVECLVELCVSRKVKCDNGFKPGAFLQVEKMLEEKLPNCGLKASPHIESRVKTLKKQFNAIMDMLTHGSGFSWDNAKKMVLCDQDVFEGWVKGHKDANGLRLKPFPHFDDLSLVFGKDRANRKGAMSAADILKELDQGEANNDIGVDDLEVEADGSHTNTVASTPSRMECSSQSRRKRAKNEDTTLINVMTRTCNALESLVGNFNQQMERENRVVGELQKLPNLNRLDILKLSQIIMNDSMKVKLLFNLDEDLKVEWVKQLLQTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DPG8</accession>
    <name>A0AA39DPG8_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Hexosyltransferase</fullName>
            <ecNumber evidence="13">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PVL29_014918</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Golgi apparatus membrane</location>
            <topology evidence="2 13">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the glycosyltransferase 31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="13"/>
    <dbReference type="EMBL" id="JARBHA010000011">
        <property type="protein sequence ID" value="KAJ9689467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DPG8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.50:FF:000027">
        <property type="entry name" value="Hexosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002659">
        <property type="entry name" value="Glyco_trans_31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214:SF351">
        <property type="entry name" value="BETA-1,3-GALACTOSYLTRANSFERASE PVG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214">
        <property type="entry name" value="BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01762">
        <property type="entry name" value="Galactosyl_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10 13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12 13" id="KW-0464">Manganese</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ9689467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="323" mass="37692" checksum="C5B226B139F89A34" modified="2024-01-24" version="1">MMNPNIQLVMMKPTKPERRRFIISSFFFVLFLCAVASINEVRFGNLLKFGSCTFFSHNSSSSASFPPLNSTSDDIRILIGILTLPDQYHRRHFLRMIYGTQSPAGAKVDVKFVFCNLTKEDQKVLVALEIMRYDDIIILNCTENMNQGKTYTYFSSLPEMLNSTEGPSPPYHYVMKADDDIYLRLDKLVESLRPLPREDLYYGYVIPCGSMDPFHHYMSGMGYLVSWDIVEWVSVSEVSRNHLEGSEDRVFGDWMRRGHRGRKRHTVKWAMYNYPDRPTACTHELWPDTIGVHLLKKQEKWIETLKYFNVTQNLKPSKLYHIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DRF8</accession>
    <name>A0AA39DRF8_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_011898</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000009">
        <property type="protein sequence ID" value="KAJ9692980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DRF8"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9692980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="210" mass="23344" checksum="1367B662B878498F" modified="2024-01-24" version="1">MYLKSQLGLPNPSVLLLLLCFGIVAAWLVSKHWISNNLLGISICIAFVSYANVMVSVAAQQASNLVHMVAELFFRTNLFGGVVRGGNFANFMMFGLVLCFNHRKSKDSVSPLDIPSAKGHKYIWYAILGYAIRLVTALAADVLTHHQQNLPLCTPGPHSCMATFGMQSQISHPFYSSSSHHKYDFHNSKTMEFVGLIHAKSIKLWWGPYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DS30</accession>
    <name>A0AA39DS30_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Transcription factor DIVARICATA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PVL29_010403</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000008">
        <property type="protein sequence ID" value="KAJ9694913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DS30"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000009">
        <property type="entry name" value="transcription factor MYB1R1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000154">
        <property type="entry name" value="Transcription factor SRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006447">
        <property type="entry name" value="Myb_dom_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01557">
        <property type="entry name" value="myb_SHAQKYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44042:SF58">
        <property type="entry name" value="DUPLICATED HOMEODOMAIN-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44042">
        <property type="entry name" value="DUPLICATED HOMEODOMAIN-LIKE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23082">
        <property type="entry name" value="Myb_DNA-binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Myb-like" evidence="7">
        <location>
            <begin position="21"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="8">
        <location>
            <begin position="24"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="128"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="7">
        <location>
            <begin position="128"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="8">
        <location>
            <begin position="136"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="118"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="184"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="126"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="186"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ9694913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="312" mass="35325" checksum="38BE504A4ECDFBC6" modified="2024-01-24" version="1">MYRGIEFLSPASYLQNSNWLFSESNGTKWTHEENKLFENALALYDKDTPDRWLKVAAMIPGKTVEDVLKQYKDLEDDISDIEAGLIPIPGYSTSSFTLEWNNNQGFDGLKQFYFPGGKRGSATRSTDQERKKGVPWTEEEHRQFLMGLKKYGKGDWRNISRNFVTTRTPTQVASHAQKYFIRQHSVGKDKRRSSIHDITTVNLTDSKSPSPDNDRPSSPDCSTTVMQLPQYPNMVGTAKPMLDQRPPNQGAAMVFNPSNGNPFLAPPCGISSNELKLHEQFLHRGTLNGPQFVPHSSIFQMPSTQQQQRLHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DUW6</accession>
    <name>A0AA39DUW6_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PVL29_008581</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000007">
        <property type="protein sequence ID" value="KAJ9696430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DUW6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ9696430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="107" mass="13047" checksum="BB8DF16AAE6499A2" modified="2024-01-24" version="1">MEERFFFRRVDADDLYVFRWVVRYRYTDVRFEEEPFERLLVERLKEFIREEMMMTPTLTHSNGDMVSGELQDGLIDGENESEESKQIDEERRKKNVDKDIETINRAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DVC2</accession>
    <name>A0AA39DVC2_VITRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PVL29_007860</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DVC2"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="51"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ9698996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001168098"/>
        </source>
    </evidence>
    <sequence length="137" mass="16172" checksum="27B0E198CE2B2B7F" modified="2024-01-24" version="1">MKKAMMATWSESEESSEEENEKEVANMCFMAIDDLDEVNSNFSDEDMHSIFEELYEDFEKLSLKNNSLKKKIQELEKELEEVKEKFSIDEISKTHLEKENEILRNENEILKKKNEWLTSSLSIFSRGQKSFEMILAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-01-24" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AA39DWI6</accession>
    <name>A0AA39DWI6_VITRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PVL29_007654</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Vitis rotundifolia</name>
        <name type="common">Muscadine grape</name>
        <dbReference type="NCBI Taxonomy" id="103349"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>rosids</taxon>
            <taxon>Vitales</taxon>
            <taxon>Vitaceae</taxon>
            <taxon>Viteae</taxon>
            <taxon>Vitis</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="BMC Biotechnol." volume="0" first="0" last="0">
            <title>Vitis rotundifolia cv Carlos genome sequencing.</title>
            <authorList>
                <person name="Huff M."/>
                <person name="Hulse-Kemp A."/>
                <person name="Scheffler B."/>
                <person name="Youngblood R."/>
                <person name="Simpson S."/>
                <person name="Babiker E."/>
                <person name="Staton M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JARBHA010000006">
        <property type="protein sequence ID" value="KAJ9698694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AA39DWI6"/>
    <dbReference type="Proteomes" id="UP001168098">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        